LOCUS       DAJVRP010000001        84548 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVRP010000001 DAJVRP010000000
VERSION     DAJVRP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.001"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            301..1734
                     /gene="cscA"
                     /locus_tag="OQN12_000001"
     CDS             301..1734
                     /gene="cscA"
                     /locus_tag="OQN12_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCS1652495.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1742..2800)
                     /locus_tag="OQN12_000002"
     CDS             complement(1742..2800)
                     /locus_tag="OQN12_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1652496.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2968..3186
                     /locus_tag="OQN12_000003"
                     /pseudo
     CDS             2968..3186
                     /locus_tag="OQN12_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3204..4532
                     /gene="dsdA"
                     /locus_tag="OQN12_000004"
     CDS             3204..4532
                     /gene="dsdA"
                     /locus_tag="OQN12_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCS1652497.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4640..6178)
                     /gene="emrY"
                     /locus_tag="OQN12_000005"
     CDS             complement(4640..6178)
                     /gene="emrY"
                     /locus_tag="OQN12_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1652498.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6178..7341)
                     /gene="emrK"
                     /locus_tag="OQN12_000006"
     CDS             complement(6178..7341)
                     /gene="emrK"
                     /locus_tag="OQN12_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1652499.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7757..8371
                     /gene="evgA"
                     /locus_tag="OQN12_000007"
     CDS             7757..8371
                     /gene="evgA"
                     /locus_tag="OQN12_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1652500.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8376..11969
                     /gene="evgS"
                     /locus_tag="OQN12_000008"
     CDS             8376..11969
                     /gene="evgS"
                     /locus_tag="OQN12_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1652501.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(12025..13170)
                     /gene="yfdE"
                     /locus_tag="OQN12_000009"
     CDS             complement(12025..13170)
                     /gene="yfdE"
                     /locus_tag="OQN12_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1652502.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13244..14188)
                     /gene="yfdV"
                     /locus_tag="OQN12_000010"
     CDS             complement(13244..14188)
                     /gene="yfdV"
                     /locus_tag="OQN12_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1652503.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14258..15952)
                     /gene="oxc"
                     /locus_tag="OQN12_000011"
     CDS             complement(14258..15952)
                     /gene="oxc"
                     /locus_tag="OQN12_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1652504.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(16006..17256)
                     /gene="frc"
                     /locus_tag="OQN12_000012"
     CDS             complement(16006..17256)
                     /gene="frc"
                     /locus_tag="OQN12_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1652505.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17769..18392)
                     /locus_tag="OQN12_000013"
     CDS             complement(17769..18392)
                     /locus_tag="OQN12_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1652506.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18689..18964
                     /locus_tag="OQN12_000014"
     CDS             18689..18964
                     /locus_tag="OQN12_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652507.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(19041..19283)
                     /locus_tag="OQN12_000015"
     CDS             complement(19041..19283)
                     /locus_tag="OQN12_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1652508.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19636..20556
                     /gene="lpxP"
                     /locus_tag="OQN12_000016"
     CDS             19636..20556
                     /gene="lpxP"
                     /locus_tag="OQN12_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1652509.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20911..20982
                     /gene="ypdK"
                     /locus_tag="OQN12_000017"
     CDS             20911..20982
                     /gene="ypdK"
                     /locus_tag="OQN12_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1652510.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(21047..22285)
                     /gene="alaC"
                     /locus_tag="OQN12_000018"
     CDS             complement(21047..22285)
                     /gene="alaC"
                     /locus_tag="OQN12_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1652511.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22662..24148
                     /locus_tag="OQN12_000019"
                     /pseudo
     CDS             22662..24148
                     /locus_tag="OQN12_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24163..24897
                     /gene="ypdB"
                     /locus_tag="OQN12_000020"
     CDS             24163..24897
                     /gene="ypdB"
                     /locus_tag="OQN12_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS1652512.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24910..25767
                     /locus_tag="OQN12_000021"
     CDS             24910..25767
                     /locus_tag="OQN12_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1652513.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25770..28265)
                     /gene="ptsP"
                     /locus_tag="OQN12_000022"
     CDS             complement(25770..28265)
                     /gene="ptsP"
                     /locus_tag="OQN12_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1652514.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28290..29327)
                     /gene="ypdE"
                     /locus_tag="OQN12_000023"
     CDS             complement(28290..29327)
                     /gene="ypdE"
                     /locus_tag="OQN12_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1652515.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29327..30410)
                     /gene="ypdF"
                     /locus_tag="OQN12_000024"
                     /pseudo
     CDS             complement(29327..30410)
                     /gene="ypdF"
                     /locus_tag="OQN12_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30425..31672)
                     /locus_tag="OQN12_000025"
     CDS             complement(30425..31672)
                     /locus_tag="OQN12_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1652516.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31694..32020)
                     /locus_tag="OQN12_000026"
     CDS             complement(31694..32020)
                     /locus_tag="OQN12_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1652517.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32239..33204)
                     /gene="glk"
                     /locus_tag="OQN12_000027"
     CDS             complement(32239..33204)
                     /gene="glk"
                     /locus_tag="OQN12_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1652518.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33408..34664
                     /locus_tag="OQN12_000028"
     CDS             33408..34664
                     /locus_tag="OQN12_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1652519.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34779..35105
                     /locus_tag="OQN12_000029"
     CDS             34779..35105
                     /locus_tag="OQN12_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1652520.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35246..36484)
                     /locus_tag="OQN12_000030"
     CDS             complement(35246..36484)
                     /locus_tag="OQN12_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1652521.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36820..38022
                     /gene="nupC"
                     /locus_tag="OQN12_000031"
     CDS             36820..38022
                     /gene="nupC"
                     /locus_tag="OQN12_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1652522.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38072..40184)
                     /locus_tag="OQN12_000032"
                     /pseudo
     CDS             complement(38072..40184)
                     /locus_tag="OQN12_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40392..40467)
                     /locus_tag="OQN12_000033"
     tRNA            complement(40392..40467)
                     /locus_tag="OQN12_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40432..40434),aa:Ala,seq:ggc)
     gene            complement(40507..40582)
                     /locus_tag="OQN12_000034"
     tRNA            complement(40507..40582)
                     /locus_tag="OQN12_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40547..40549),aa:Ala,seq:ggc)
     gene            40803..41162
                     /locus_tag="OQN12_000035"
     CDS             40803..41162
                     /locus_tag="OQN12_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1652523.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41164..41556
                     /locus_tag="OQN12_000036"
     CDS             41164..41556
                     /locus_tag="OQN12_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1652524.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41608..43023)
                     /gene="gltX"
                     /locus_tag="OQN12_000037"
     CDS             complement(41608..43023)
                     /gene="gltX"
                     /locus_tag="OQN12_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1652525.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43282..43357
                     /locus_tag="OQN12_000038"
     tRNA            43282..43357
                     /locus_tag="OQN12_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43315..43317,aa:Val,seq:tac)
     gene            43402..43477
                     /locus_tag="OQN12_000039"
     tRNA            43402..43477
                     /locus_tag="OQN12_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43435..43437,aa:Val,seq:tac)
     gene            43482..43557
                     /locus_tag="OQN12_000040"
     tRNA            43482..43557
                     /locus_tag="OQN12_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43515..43517,aa:Lys,seq:ttt)
     gene            43588..43713
                     /locus_tag="OQN12_000041"
     ncRNA           43588..43713
                     /ncRNA_class="other"
                     /locus_tag="OQN12_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43989..44330
                     /locus_tag="OQN12_000042"
     CDS             43989..44330
                     /locus_tag="OQN12_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1652526.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44321..45247)
                     /locus_tag="OQN12_000043"
     CDS             complement(44321..45247)
                     /locus_tag="OQN12_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1652527.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45337..46335
                     /locus_tag="OQN12_000044"
     CDS             45337..46335
                     /locus_tag="OQN12_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1652528.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46332..46550)
                     /locus_tag="OQN12_000045"
     CDS             complement(46332..46550)
                     /locus_tag="OQN12_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1652529.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46552..48567)
                     /gene="ligA"
                     /locus_tag="OQN12_000046"
     CDS             complement(46552..48567)
                     /gene="ligA"
                     /locus_tag="OQN12_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1652530.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48638..49624)
                     /gene="zipA"
                     /locus_tag="OQN12_000047"
     CDS             complement(48638..49624)
                     /gene="zipA"
                     /locus_tag="OQN12_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1652531.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49854..50615
                     /gene="cysZ"
                     /locus_tag="OQN12_000048"
     CDS             49854..50615
                     /gene="cysZ"
                     /locus_tag="OQN12_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1652532.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50800..51771
                     /gene="cysK"
                     /locus_tag="OQN12_000049"
     CDS             50800..51771
                     /gene="cysK"
                     /locus_tag="OQN12_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1652533.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52155..52412
                     /gene="ptsH"
                     /locus_tag="OQN12_000050"
     CDS             52155..52412
                     /gene="ptsH"
                     /locus_tag="OQN12_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1652534.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52457..54184
                     /gene="ptsI"
                     /locus_tag="OQN12_000051"
     CDS             52457..54184
                     /gene="ptsI"
                     /locus_tag="OQN12_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1652535.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54225..54734
                     /gene="crr"
                     /locus_tag="OQN12_000052"
     CDS             54225..54734
                     /gene="crr"
                     /locus_tag="OQN12_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1652536.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54776..55627)
                     /gene="pdxK"
                     /locus_tag="OQN12_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54776..55627)
                     /gene="pdxK"
                     /locus_tag="OQN12_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1652537.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55732..56106
                     /locus_tag="OQN12_000054"
     CDS             55732..56106
                     /locus_tag="OQN12_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1652538.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56139..56862
                     /locus_tag="OQN12_000055"
                     /pseudo
     CDS             56139..56862
                     /locus_tag="OQN12_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(57066..57977)
                     /gene="cysM"
                     /locus_tag="OQN12_000056"
     CDS             complement(57066..57977)
                     /gene="cysM"
                     /locus_tag="OQN12_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS1652539.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(58111..59208)
                     /gene="cysA"
                     /locus_tag="OQN12_000057"
     CDS             complement(58111..59208)
                     /gene="cysA"
                     /locus_tag="OQN12_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1652540.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59198..60073)
                     /gene="cysW"
                     /locus_tag="OQN12_000058"
     CDS             complement(59198..60073)
                     /gene="cysW"
                     /locus_tag="OQN12_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1652541.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(60073..60906)
                     /gene="cysT"
                     /locus_tag="OQN12_000059"
     CDS             complement(60073..60906)
                     /gene="cysT"
                     /locus_tag="OQN12_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1652542.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60906..61922)
                     /gene="cysP"
                     /locus_tag="OQN12_000060"
     CDS             complement(60906..61922)
                     /gene="cysP"
                     /locus_tag="OQN12_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1652543.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62226..63017)
                     /gene="ucpA"
                     /locus_tag="OQN12_000061"
     CDS             complement(62226..63017)
                     /gene="ucpA"
                     /locus_tag="OQN12_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1652544.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63204..64004)
                     /gene="murR"
                     /locus_tag="OQN12_000062"
     CDS             complement(63204..64004)
                     /gene="murR"
                     /locus_tag="OQN12_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1652545.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            64168..65064
                     /gene="murQ"
                     /locus_tag="OQN12_000063"
     CDS             64168..65064
                     /gene="murQ"
                     /locus_tag="OQN12_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1652546.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            65068..66492
                     /gene="murP"
                     /locus_tag="OQN12_000064"
     CDS             65068..66492
                     /gene="murP"
                     /locus_tag="OQN12_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCS1652547.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66672..67571)
                     /gene="yfeX"
                     /locus_tag="OQN12_000065"
     CDS             complement(66672..67571)
                     /gene="yfeX"
                     /locus_tag="OQN12_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1652548.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67667..68242)
                     /locus_tag="OQN12_000066"
     CDS             complement(67667..68242)
                     /locus_tag="OQN12_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1652549.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68303..68752)
                     /locus_tag="OQN12_000067"
     CDS             complement(68303..68752)
                     /locus_tag="OQN12_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1652550.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68739..69164)
                     /locus_tag="OQN12_000068"
     CDS             complement(68739..69164)
                     /locus_tag="OQN12_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1652551.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69378..70247
                     /gene="amiA"
                     /locus_tag="OQN12_000069"
     CDS             69378..70247
                     /gene="amiA"
                     /locus_tag="OQN12_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1652552.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70251..71150
                     /gene="hemF"
                     /locus_tag="OQN12_000070"
     CDS             70251..71150
                     /gene="hemF"
                     /locus_tag="OQN12_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1652553.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71156..72208)
                     /gene="eutR"
                     /locus_tag="OQN12_000071"
                     /pseudo
     CDS             complement(71156..72208)
                     /gene="eutR"
                     /locus_tag="OQN12_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72254..72754)
                     /gene="eutK"
                     /locus_tag="OQN12_000072"
     CDS             complement(72254..72754)
                     /gene="eutK"
                     /locus_tag="OQN12_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1652554.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72767..73426)
                     /gene="eutL"
                     /locus_tag="OQN12_000073"
     CDS             complement(72767..73426)
                     /gene="eutL"
                     /locus_tag="OQN12_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1652555.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74608..75399
                     /locus_tag="OQN12_000074"
     CDS             74608..75399
                     /locus_tag="OQN12_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652556.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            76119..76514
                     /locus_tag="OQN12_000075"
     CDS             76119..76514
                     /locus_tag="OQN12_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1652557.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76532..76795
                     /locus_tag="OQN12_000076"
     CDS             76532..76795
                     /locus_tag="OQN12_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652558.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76818..77360
                     /locus_tag="OQN12_000077"
     CDS             76818..77360
                     /locus_tag="OQN12_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652559.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77499..77786
                     /locus_tag="OQN12_000078"
     CDS             77499..77786
                     /locus_tag="OQN12_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652560.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77825..78031
                     /locus_tag="OQN12_000079"
     CDS             77825..78031
                     /locus_tag="OQN12_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652561.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78343..78828
                     /locus_tag="OQN12_000080"
     CDS             78343..78828
                     /locus_tag="OQN12_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1652562.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78809..79087
                     /locus_tag="OQN12_000081"
     CDS             78809..79087
                     /locus_tag="OQN12_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652563.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            79080..80540
                     /locus_tag="OQN12_000082"
     CDS             79080..80540
                     /locus_tag="OQN12_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1652564.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80574..81383
                     /gene="murI"
                     /locus_tag="OQN12_000083"
     CDS             80574..81383
                     /gene="murI"
                     /locus_tag="OQN12_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1652565.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81502..82196)
                     /locus_tag="OQN12_000084"
     CDS             complement(join(81502..81950,81950..82196))
                     /locus_tag="OQN12_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1652566.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82715..84088)
                     /gene="eutA"
                     /locus_tag="OQN12_000085"
     CDS             complement(82715..84088)
                     /gene="eutA"
                     /locus_tag="OQN12_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCS1652567.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(84063..84542)
                     /gene="eutP"
                     /locus_tag="OQN12_000086"
     CDS             complement(84063..84542)
                     /gene="eutP"
                     /locus_tag="OQN12_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS1652568.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
BASE COUNT        21959 a        21406 c        20062 g        21121 t
ORIGIN      
        1 caagtgagcg tttccggatt cttacacagc cactggatcg gtcaactgat ccttaactga
       61 tcggcattaa tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt
      121 gataaacctc tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca
      181 aaacgccata gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact
      241 atatttttat aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa
      301 atgacgcaat ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg
      361 ggtaacactt tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca
      421 aacggcctga tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc
      481 gaacactggg gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag
      541 catgagccta ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt
      601 agtgctgtcg atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat
      661 ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt
      721 attcatttcg agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc
      781 gatcctaaag tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca
      841 ggcaacacgg ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat
      901 cgcgtactgg cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc
      961 agccttggcg atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac
     1021 agttaccgaa atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt
     1081 tttgcacaat ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa
     1141 agctttttag cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg
     1201 ccaatgccct caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca
     1261 gagagcaatg gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag
     1321 catcaatctg tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa
     1381 gcagttgaga ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta
     1441 cagctcggca ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg
     1501 cggtattacc cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac
     1561 acgctcgccc taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg
     1621 gaagcggtga tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat
     1681 gcctcccacg gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat
     1741 atcaggtgga acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac
     1801 gaccttcaat aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg
     1861 tggtcagcgg cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag
     1921 cgacatcctg cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg
     1981 cgcgatcgtt accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat
     2041 tgagtaaact tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca
     2101 cctcagccag atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc
     2161 ctgttgccaa cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga
     2221 gcaaatgctg tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg
     2281 ctgggtcatc cgccacacag ttcgccaata caatattttc accatacaga gactcaggca
     2341 gcgtgatatg tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca
     2401 gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc
     2461 agccgaactc gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg
     2521 tagccgtgtc ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct
     2581 tacgggcgga aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac
     2641 gcgttgcagg acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca
     2701 ctcccgccag gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac
     2761 gggcactgta gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag
     2821 caatacaata aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct
     2881 cgccaacgag taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg
     2941 gagaagaaaa tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa
     3001 attattgcga ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt
     3061 ttctggctgg tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg
     3121 acagcgacat ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc
     3181 atctaagcgc aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt
     3241 atccgttggt aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga
     3301 cctcattggc tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc
     3361 atgcgcgctt atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca
     3421 ctggggggat tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa
     3481 aggaatatca acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca
     3541 tttccggctc tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac
     3601 tggctctgga agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg
     3661 agtttaaaca gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt
     3721 tatcaatcgg cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg
     3781 atgcccgggc atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg
     3841 agcaagatta tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact
     3901 gtttctttat tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc
     3961 agcgtcttaa agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt
     4021 ttgtctatct gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac
     4081 tggcgtttgg cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt
     4141 tgttaggcgt ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca
     4201 accttaccgc agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa
     4261 tggagcgtct gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg
     4321 gctggctggc gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg
     4381 gacctcagcg cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac
     4441 aattgcgtaa tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag
     4501 agatgaatca atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct
     4561 ttccctgggt gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta
     4621 taaccccctc cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc
     4681 aaaccaaaac cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag
     4741 aaatagaaag tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg
     4801 aaccatagta tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga
     4861 tggttgctgt caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca
     4921 acgacgtacc aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg
     4981 caaatttatt atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg
     5041 caacggcgaa tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca
     5101 taaatgtcac agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta
     5161 acacccgcat gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca
     5221 tgatgccgat gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct
     5281 ggagtaactg cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac
     5341 tcacaatacc aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct
     5401 ctgaggtcga ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata
     5461 ttattattgt actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca
     5521 agccaccaac accgagcact aacagggtca gtcttggtag attcattttt accggtgaag
     5581 tttcagtttc tcttccttta agtaaggtta agcataatgt caggacgata atccccatag
     5641 ggacattgat taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata
     5701 tcggcccaca tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc
     5761 ttttttctgg tggataattc cttaataata aactctgtga cagtggaatt aacggccccg
     5821 ccattaatcc ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac
     5881 acattaagga agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta
     5941 ttctttgtgc cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg
     6001 tgataaccca ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg
     6061 cgacgttaga aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag
     6121 taacgcacca taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta
     6181 aagttgtcca ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat
     6241 aactaaagcc ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat
     6301 ctcagcaatg tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa
     6361 ggggtgttcc atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat
     6421 tttgatccag ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc
     6481 catattgatc cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat
     6541 aatattgact gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt
     6601 aacccacatt tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc
     6661 gccgacctga acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg
     6721 tttaagcacc aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg
     6781 gtttaatggt gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt
     6841 cagtgccgct ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat
     6901 aaccccctgc ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg
     6961 aatacgtgct gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt
     7021 ttgccgaaca atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt
     7081 tttatccagt gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac
     7141 tacagtgaca ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc
     7201 gtcatctgta ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa
     7261 cgcaataaat aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt
     7321 atttgaatta atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg
     7381 aatgaaaaga gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag
     7441 gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg
     7501 cttttgcaat acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg
     7561 aaactataaa tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact
     7621 atttatatgg tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt
     7681 gagaaaatga gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt
     7741 gcaaagggaa taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc
     7801 aattcgtaat ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg
     7861 aagcgccgtt cagcgggtgg aaacacttaa gcctgatata gtcatcattg atgtcgatat
     7921 ccccggagtt aacggtatcc aagtgttaga aacgctgagg aagcgccaat atagcggaat
     7981 tattattatc gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg
     8041 cgctaatggt ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc
     8101 tgcaaaaaat ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac
     8161 gtccgaccag caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat
     8221 tcttgatggc aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt
     8281 cagcacgtat aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta
     8341 cacattcgcg caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc
     8401 ttctctgttg tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc
     8461 gtggcatcag tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat
     8521 tacgttggtt agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta
     8581 ccttgttgca taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc
     8641 ttttaaaaag agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag
     8701 caatggacgc gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc
     8761 cgcctcttaa taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg
     8821 taaccaccct tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc
     8881 gggtagcaaa ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta
     8941 tctcttttac aaatttatat caggcattag catccgtctc agctgggcac aatgattact
     9001 ttattggtag taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa
     9061 atgtagtgaa atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat
     9121 ctgtcattct taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct
     9181 atgaagtatc acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag
     9241 aactcactga acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa
     9301 atccttactc gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg
     9361 gggacattct taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt
     9421 cacacaatat ccatgctgga acacaactta gccccggagg atgggatata atacctggcg
     9481 ctatttatag tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc
     9541 cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga
     9601 aagttgccat tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg
     9661 ttgaatggat acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac
     9721 ttgatgctct ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta
     9781 atgaacttta tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc
     9841 ctcgcggaga accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa
     9901 gcgaagttct gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat
     9961 gggacctata tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca
    10021 gtagcctttt atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg
    10081 gtgatttaga aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa
    10141 cttatgttgt aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt
    10201 tcactgcgga ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta
    10261 aagatgtttt ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat
    10321 acacacaggt atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata
    10381 cattatgcaa tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta
    10441 ctgaaacgcg tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta
    10501 ccgtagcaaa aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct
    10561 ctattatggg cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg
    10621 aggcgatttc acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc
    10681 ttgatgtcga caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc
    10741 ctactttagt ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg
    10801 cattaagttg cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt
    10861 ttaagcaggt cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag
    10921 taaaaattac gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga
    10981 ttatggattc tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca
    11041 gccaaacaag tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag
    11101 aattaattaa aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa
    11161 catttacgat cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag
    11221 cagaacaacc catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga
    11281 ccaacaggct attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca
    11341 ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg
    11401 acgttaatat gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt
    11461 cttccttacc catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt
    11521 taagttgcgg catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac
    11581 atttaagtca gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg
    11641 aagccctgaa aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt
    11701 tccagcatga aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata
    11761 acagaacttt ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc
    11821 aaaagttgat taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc
    11881 ctgaaattct tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga
    11941 ttgctgtttt ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga
    12001 gctatttacc agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg
    12061 ctggtcgagc gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat
    12121 cggattaccc ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg
    12181 tggcaaatta atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg
    12241 tattttggct aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt
    12301 aagaatagcc tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag
    12361 ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat
    12421 cgtaatcggc ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt
    12481 tcccaggcgt tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa
    12541 actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg
    12601 gccataaagt gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag
    12661 agaggtacca acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact
    12721 cattgcctga atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa
    12781 acctgacgat gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa
    12841 ccccagtttt tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag
    12901 catatttata aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt
    12961 gccatgatta ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg
    13021 ggtatcatca ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag
    13081 aagttgagtt ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc
    13141 aaacggccct ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg
    13201 aaaagacact tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac
    13261 tgacataaat ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg
    13321 ctgtaccagt acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg
    13381 gcggtaatgc gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca
    13441 taccaacgag aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa
    13501 tttcggcact gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta
    13561 cccctgagtt agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta
    13621 ccccaaccag caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg
    13681 cagaaattaa tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca
    13741 gcaaatacag accaatagga atagtaattg cgttaacaat aatagaaata attgctacca
    13801 ctaaacctgt tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc
    13861 caatggtagg tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa
    13921 aaaatttgta gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata
    13981 caagggtcag acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa
    14041 atagagccgc aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt
    14101 ctgaaaaagt ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca
    14161 ataaatcgcc aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat
    14221 cgcataatga gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg
    14281 ggttaagttt ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa
    14341 taatggtcgg tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg
    14401 tggtgacgtt atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt
    14461 gcaacagatc cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt
    14521 agatgccgcc attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt
    14581 caatttccat cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac
    14641 cagaagtcac gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac
    14701 cacaatccag acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg
    14761 tatttgcacc ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag
    14821 cacttaatgc attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt
    14881 tttgtgcatt ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa
    14941 atgtgttttg tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga
    15001 ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa
    15061 actgtgtatc tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac
    15121 caacaagcat gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa
    15181 gtggatgcgt atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag
    15241 cactttcaat aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa
    15301 ggataattaa tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg
    15361 ggcatggcaa taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt
    15421 ctttttccat cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc
    15481 gacccgatac agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat
    15541 taacgcgaaa tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat
    15601 aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg
    15661 gaaaaccatt taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag
    15721 aaactgtcag gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc
    15781 ctgccgactg ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg
    15841 ccatatccgt cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta
    15901 atgcttcaac gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata
    15961 acctcgaaat ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt
    16021 ttgcttcatt gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt
    16081 atgttcacct aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg
    16141 acaaccaacg gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact
    16201 ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat
    16261 atcaaactga gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc
    16321 gatttcagca aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg
    16381 atcggtaatc cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg
    16441 aatagtgaaa taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca
    16501 gccaggctga ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc
    16561 attcggatac tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg
    16621 taatttcacg cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc
    16681 acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc
    16741 ggtgttgctg tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt
    16801 agtggatgcc gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata
    16861 aggcgaacac tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg
    16921 aatatgctcc caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa
    16981 gatatcagct tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt
    17041 taactcaata gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg
    17101 aatatctcgc agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac
    17161 gtcagcgcca aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa
    17221 atcgagaact ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa
    17281 tatgtgttaa tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata
    17341 tcttcgtagc ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat
    17401 cgccacttcc aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa
    17461 atattgattc gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt
    17521 aagaagcgcc ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa
    17581 gcataacata ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa
    17641 taaaatgcgt gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta
    17701 ttgtggtcat aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc
    17761 tgtcagcatt agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct
    17821 ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca
    17881 agtgcattac gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg
    17941 gccaggcgaa gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca
    18001 atttttatcg cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat
    18061 gcattaatga caatatattg gtcatcattg agattggtct ttttacccgg cttagcgaac
    18121 tttgcccatt cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt
    18181 gccttttccg gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca
    18241 tataaacctt gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc
    18301 tgttgtgccg ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct
    18361 gtgaccatcg tagccataat taaacgtttc atacttacct cattctttaa cagttttaga
    18421 aaacgccgcc atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt
    18481 gaaagggtta tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt
    18541 aggaatatgc ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat
    18601 tatattgatg taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct
    18661 cagtgaacca ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt
    18721 tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag
    18781 tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa
    18841 acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct
    18901 cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg
    18961 atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc
    19021 aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag
    19081 ccacaagccg gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac
    19141 cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc
    19201 gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg
    19261 agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt
    19321 cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga
    19381 aaacagcacg ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa
    19441 taatcagtag ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct
    19501 cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg
    19561 cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat
    19621 ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc
    19681 tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct
    19741 gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt
    19801 gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc
    19861 gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg
    19921 gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac
    19981 cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg
    20041 ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca
    20101 cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg
    20161 atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta
    20221 tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg
    20281 gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc
    20341 atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc
    20401 ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt
    20461 atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa
    20521 acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc
    20581 ctcaaatcgg ccatgataac ctcatgattt ttatagcttt tttcttaagt tttttccttc
    20641 gctgaatatc taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa
    20701 gttactaaaa gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag
    20761 cgccgtctcg agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc
    20821 ttttaaggtc gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg
    20881 cgtaacagta attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt
    20941 atggtcatcg tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga
    21001 aaaacaggag ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt
    21061 cgtgaatatg tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc
    21121 ctctgatggc ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat
    21181 caccatagtc gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct
    21241 tcttggcaaa ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat
    21301 acatcgaagc cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata
    21361 catcacgacg gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga
    21421 gtgccgcaat cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga
    21481 ttcgcgccag ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg
    21541 ccatgttgta gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca
    21601 cctgcataat tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat
    21661 ggaccaccag cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt
    21721 ccacgcattg cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat
    21781 aactttcacg aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca
    21841 ctgagcgtac ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat
    21901 tcggcaccag caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct
    21961 ctttcgaacc aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat
    22021 cctgatacca gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac
    22081 catgcgtgtc cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg
    22141 caccgtccgg gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca
    22201 ttttcagttc agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc
    22261 gacgttcagg gcgagtgtca gccataattt tctcagatta acgttagcgc ccggaccgtc
    22321 cgagcgacgc tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc
    22381 cagcgtttta gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg
    22441 aaatttttct acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata
    22501 aatttaccag ctagaagtgc attatcaaga gatacattgc taatacacca tttatctttc
    22561 aatccccttt taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag
    22621 gttacctatc atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca
    22681 tgctgctggc ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc
    22741 gtatccgcct gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg
    22801 ccgttaccgc cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag
    22861 aaggctcgct ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc
    22921 cgtgggtagg catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg
    22981 gcggcgtgac ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct
    23041 ggataaacct gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct
    23101 accgctgttt cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga
    23161 tgacattcac gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg
    23221 cgtgggtgaa cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc
    23281 gatgaattac ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat
    23341 tcattccatg ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat
    23401 ttactactgc cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc
    23461 gcaaatcatc tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa
    23521 taaggcagag cttcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct
    23581 gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa
    23641 tctgtcgcgt tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa
    23701 aaaagagctg tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa
    23761 gctgacggtt atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat
    23821 ccagccgttg gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt
    23881 tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg
    23941 gcacggcatc gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa
    24001 aattggcctg ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat
    24061 ccgccgcctg gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt
    24121 cgcctcacag gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt
    24181 gaagacgaat tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg
    24241 gagattgtcg gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc
    24301 gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa
    24361 aacatcagcc agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat
    24421 gcggtagaag cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca
    24481 cgtattaccg ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt
    24541 acgcctgccg cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga
    24601 ataatcgtca cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt
    24661 gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa
    24721 ctgccgccgt cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata
    24781 cgcgaaatcg aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt
    24841 gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg
    24901 ggaactttca tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc
    24961 agcggcctag tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc
    25021 tcgttaccgg tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc
    25081 gaatacggca acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt
    25141 attccggcac gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg
    25201 gtgttcgcac cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg
    25261 catcctgctc gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt
    25321 accgccctca cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg
    25381 ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg
    25441 cgcggcgatt ttctctatca cagcatttgt aactgggttc aggataatta tgcccagccg
    25501 ctcacccgcg agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg
    25561 tttgctcagc atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag
    25621 gcgaggatga ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt
    25681 tttccggata gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga
    25741 gagtacagcg cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc
    25801 aggatacttt gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt
    25861 ttacgcgcca gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc
    25921 agcatgatca ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc
    25981 cgggcaatgc tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg
    26041 ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca
    26101 aacggatgtt cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc
    26161 tctttattgc taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg
    26221 tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca
    26281 catgcctgac gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc
    26341 accgccggaa tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc
    26401 ggcagataac ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga
    26461 tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat
    26521 agcggcgata cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg
    26581 gagccgatac tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac
    26641 ggcacttcca ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta
    26701 agctcaacga tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga
    26761 accatcggga tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt
    26821 tgagtgcgga acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac
    26881 gggttctctt cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc
    26941 gtgcggaaga taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat
    27001 tgctcctgct cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc
    27061 ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc
    27121 gccgcaatat caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc
    27181 tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg
    27241 tcattcgggt taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca
    27301 taacgggcaa tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc
    27361 agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc
    27421 aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt
    27481 tccagcacca gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg
    27541 ctgatatcgc gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt
    27601 ttggcgcaaa cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct
    27661 gtcatcaggc gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg
    27721 ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca
    27781 agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga
    27841 taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc
    27901 agcacattgc cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc
    27961 gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg
    28021 tactcttcca gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa
    28081 ctgtcattaa ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt
    28141 gcgttctggc gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta
    28201 agttcccacg ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt
    28261 aacataaccg ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc
    28321 tcacgcgtaa gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg
    28381 caatcggcaa tcgatgccgc gcaatgtccg tggcgggtgg caggccctat taccaatgtg
    28441 ggtacgccag taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata
    28501 tccgcctgca acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt
    28561 ggcggcgcaa tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga
    28621 tggttagccg cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg
    28681 acgtccggcg acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg
    28741 gaacttgcca ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac
    28801 gtcaccagca gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga
    28861 gggaggacct gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc
    28921 atcacttcgt cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc
    28981 tgccggtcgc cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc
    29041 ggctgcagct ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct
    29101 tcgcgggaga tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc
    29161 ttcggacctg tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact
    29221 tctttttgca ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag
    29281 gcgatcgcat ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg
    29341 tgagcaacac tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca
    29401 caacatcttc gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca
    29461 gcaacatgcc tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga
    29521 cttcaatgcc gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg
    29581 taatgacccg gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag
    29641 agattgccgc gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt
    29701 cggcgctcac cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga
    29761 tacagcgcac caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct
    29821 ttgccgtgcg gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt
    29881 tctgcgccct gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc
    29941 cccgcctgaa taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg
    30001 cggattatct ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta
    30061 accagtttcg cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg
    30121 ccttcaaaac cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta
    30181 agcgtgttcg tcgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca
    30241 acgaaatagc gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct
    30301 gatccggtgg agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca
    30361 tccagttgtt gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc
    30421 cttcttacag gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca
    30481 ccaccatcag cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaggt
    30541 tagtgaccag cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc
    30601 agacagctgc ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg
    30661 ggctttccag cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg
    30721 cggtcttacc ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt
    30781 caataatggt cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg
    30841 tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga
    30901 tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc
    30961 ccttctcacc tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg
    31021 gagtgatgac gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa
    31081 atgtggtttt gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca
    31141 caaggtaacc ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg
    31201 aagaggtcgc ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag
    31261 acatcaaccc gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca
    31321 caaatgcagc aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca
    31381 gccaggcaaa tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat
    31441 ccatgatgcc gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag
    31501 cgaggatcac accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat
    31561 gctgcgggag ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg
    31621 ccgcaccgga tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta
    31681 tccctgtcga atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc
    31741 ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac
    31801 gttcgttatc ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat
    31861 cctgcgccgt caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat
    31921 aaccggcttc taccgccgct tcttccagca cctgagcggc cataaaagtg tgagctaggc
    31981 ccattgggca ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg
    32041 atagttgacg agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg
    32101 catttactgg agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg
    32161 gacacaaaga aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc
    32221 cgatataaca ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga
    32281 accgagaaga cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc
    32341 tttaaagcgc cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag
    32401 gaagcgcggc acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc
    32461 cagattgccg ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc
    32521 ggtgcagctg tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag
    32581 gcggttgtca gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac
    32641 gcgctccgcc gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc
    32701 ttcactattc ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt
    32761 atcgacatgg accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg
    32821 cttaccttcg accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg
    32881 gatcgccatc gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt
    32941 ctttttcatt tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc
    33001 ggtaattggg caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag
    33061 ataaacgcga atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg
    33121 cgagatttca ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc
    33181 accgactaat gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt
    33241 ctcacactgt aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa
    33301 tatcgatctg ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt
    33361 tgcaagctgg gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc
    33421 gagccagaac catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc
    33481 ttattctgat tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac
    33541 tgccgggaac tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc
    33601 taacgggtat tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc
    33661 ccgcctgtga accgctgatc ggcgcaccgg ttccgccctc tgcgctacct ggacttatcg
    33721 tagcattaat tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga
    33781 ccgtcaatat cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa
    33841 tggagtggac tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg
    33901 cggcggcgtt gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc
    33961 gtctttttgc gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc
    34021 atccccattt ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca
    34081 gcggtgcaat tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac
    34141 cacggttgca cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg
    34201 gatttattct cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg
    34261 atgagatgca gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg
    34321 cggtaattaa acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa
    34381 tcttcccggc agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg
    34441 ccgttccagc ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac
    34501 gcgatggctg gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac
    34561 cgctgctctg tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga
    34621 tggtcaatcg tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct
    34681 ttatgattta agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa
    34741 caggccgctg acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg
    34801 cggccgccct gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc
    34861 catcgatcaa attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc
    34921 actggcgaga tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc
    34981 gtcatgataa tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc
    35041 gtgaaggctg gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc
    35101 attaaacgcg tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg
    35161 cctacaaacc ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat
    35221 agtgccagat gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga
    35281 tattcagcgc cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt
    35341 tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca
    35401 gggcgatacc aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc
    35461 ccatcagaat gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga
    35521 tatggaagcg aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg
    35581 aggacagtcc ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca
    35641 gcggttgcag cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt
    35701 ggaacgccgc cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg
    35761 cgatagccac atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat
    35821 gctgagtgag cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc
    35881 ctgctgccag gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca
    35941 gctgcgccag gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca
    36001 acagtaaccc gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa
    36061 tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga
    36121 gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt
    36181 ctgcctgaac ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg
    36241 ccagattttt accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca
    36301 tcaggttggc ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa
    36361 tattggtcgc aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca
    36421 ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct actctcaacg cgatagttcg
    36481 tcatcttgtg cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg
    36541 attatcaaat tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta
    36601 cgaaaagatt gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact
    36661 ttcagcttcc atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt
    36721 cacaggacgt cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat
    36781 gtgcacggaa atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt
    36841 gtactggcac ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa
    36901 attcgtatcc gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc
    36961 ctgaactccg acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg
    37021 ctcggatttg ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg
    37081 gcattcttct tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt
    37141 ctccagcaca ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa
    37201 gtcaacggta tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag
    37261 tcagaaaact ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac
    37321 accatggctg ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc
    37381 atgctggaac cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc
    37441 gtgctgtcgc tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc
    37501 aacctgcacg aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc
    37561 aaagttgcca ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg
    37621 aacgcactgt ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc
    37681 ctgggctaca tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg
    37741 caagtgggca gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat
    37801 ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta
    37861 gtttccttcg ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat
    37921 gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg
    37981 gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc
    38041 cggggatcat tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa
    38101 tcaaataccc ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg
    38161 tctcgacaaa ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc
    38221 tcacaatcat cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt
    38281 tgatgatatc agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt
    38341 catcaatcgc aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt
    38401 ctgcgttaga aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt
    38461 gataacgctt aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg
    38521 gcattaaatt gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact
    38581 tcaacaagga ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa
    38641 ggggcagaaa cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct
    38701 catcataacc ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct
    38761 gatctaacgc cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa
    38821 ccatctcctc tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca
    38881 gccagcttaa ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc
    38941 aggcagcacc atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca
    39001 ggatgttaac catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca
    39061 gttcactacc cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac
    39121 ggcaaattga gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact
    39181 caagattatc aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt
    39241 ccaacgcacg aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc
    39301 ggcgattaag ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa
    39361 aggaaatcag caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa
    39421 agttctggtt gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg
    39481 gatagcgaag attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac
    39541 cggcaataaa gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg
    39601 ccagccagct taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact
    39661 gcggtgaaaa ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag
    39721 gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg
    39781 ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat
    39841 ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca
    39901 aacgtctggc tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga
    39961 ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag
    40021 attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg
    40081 gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt
    40141 gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca
    40201 ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg
    40261 gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac
    40321 gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag
    40381 ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa
    40441 gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc
    40501 aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct
    40561 ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata
    40621 tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa
    40681 tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt
    40741 tcctgtaaag tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg
    40801 ttatgttcaa ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc
    40861 agaccattaa taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc
    40921 agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg
    40981 ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag
    41041 tattgctggt gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct
    41101 tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat
    41161 aatatgaaaa gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg
    41221 gctaaacaaa ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc
    41281 ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt
    41341 cagaacacgc cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct
    41401 gaatacgctc ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat
    41461 atgacagaca ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat
    41521 ttcctcgccc gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct
    41581 cctgccgttt atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc
    41641 cagcgctttg ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc
    41701 cagtgctgga gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc
    41761 catacccact tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt
    41821 ccagtcagta atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg
    41881 acgcagatgt tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg
    41941 gcagctctgt gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc
    42001 agccagctgc gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg
    42061 agtagcaaca tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt
    42121 gtcggtgttg aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat
    42181 ctcttcacga gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt
    42241 cagcagtgct tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg
    42301 acgtttggac agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg
    42361 caccggcgct ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc
    42421 ttcgccacgg ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt
    42481 ataggttggg gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa
    42541 ctcgatcgga ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa
    42601 acgtacaaca cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc
    42661 ataacgcggc ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt
    42721 agagcaatag catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta
    42781 gcgatcaaaa cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt
    42841 catgccatcc ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc
    42901 aatacgcagc acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt
    42961 acgcgcgccg ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt
    43021 catgaaatgg ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag
    43081 tgggcgatat tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg
    43141 cattattgtt ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa
    43201 atttacgacg aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta
    43261 taatgcgact ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag
    43321 gagggggtcg gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg
    43381 tgtagcaaga aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag
    43441 gagggggtcg gcggttcgat accgtcatca cccaccactt cgggtcgtta gctcagttgg
    43501 tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg
    43561 taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc
    43621 agcaggtttg agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg
    43681 cgagcgaagc gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct aaaggcgctt
    43741 ttttgctatc tgcgatactc aaagattcga acctgcagca ggtttgagtt gagcgcagcg
    43801 aaacaacgga gccgctcgcg gcgacggccc gaaaggcgag cgaagcgagt catcctgcac
    43861 gacccaccaa tgcaaaaaag cgccctaaag gcgctttttt gctatctgcg atttgtaaga
    43921 ttgatttttc ttctcaagtt ccttgttctt ctaccgatat tacctcgtac agtgacgagg
    43981 actgaacaat ggccactatc tcttcaactt caattccttc catacaaaca cagagtagta
    44041 acagggcatc acaaggtagc gatgttgctt cacaaatcgc gcggataagt cagcaaatca
    44101 tcaaactcac tcaacaaatt aaagagattg tagacactag cggcagtgct gaagacaagc
    44161 aaaaacaggc cgaattgatt caacagcaaa tcaccctact ggaaacacaa ctggcgcagt
    44221 tgcagaaaca gcaggcagaa aaggcgcagg agaaagagca gcgtttgtct ctgaatgtat
    44281 cccttctaaa tcctgttgaa aaaacgactc atatcgatat ctatatctga tacaacggat
    44341 ccccttcccg accagccatt agcgcattgc cctggtctcg taccacgtcc cataacgctt
    44401 cagcggcggt ggaaagtgaa cggttcttcc tgcgcaccag catcaactgg cgttcaacca
    44461 ccggcgtaat gcgcttaacc accagtgggc taccttcagg caatggtagc gccagtgcag
    44521 gaagaatact gatgccgatg cctgcggcta ccatcgggaa cagcgtcgcc ggatgtccaa
    44581 tctcctgcac aatattcgcc tgaataccat tgcgcgccag cgctgcgtca atcagcggtc
    44641 ggctgcctga cgcgtaatcc tgcaacacca atttcgctcc ctgtaatgcc tgccagggta
    44701 cataatcttc cacggccaaa gcgctatcgc gatggcaaag cagaaagaaa ggctcggaaa
    44761 gaatcgcttc acattgcaga tcgcccactg ggcctggatc gatgacgatg ccaaagtcga
    44821 catctccctg gcgaatactt tccatcaccc actgctgcgg acgatcatgc aggacaaact
    44881 gaatatctgg atagcggcga tggctttcag caatgcattg cggaataaga tgcgcggaaa
    44941 tggtctggct ggcagcgacc cgaacttttc cgctcagttg ttgccccata cgcccggtat
    45001 cgcgcaacgt gctgttaagt tcatccagca gtcgctcaag acgcaaagcc aactgctgcc
    45061 ctgcatctgt aagcaccact tcacgcgtgg ttctgtccag caggcgaaca ccagtatgat
    45121 tttccagctc cttcacactg tgacttactg ccgactggct caagccgata cgctctcctg
    45181 cacgactaaa acttttctcc tgcgctactg tgacgaaaac ctttaattgt tttaaagaat
    45241 aattcatctg ttttcttcat gaatggatgc aataaatcaa ttttatttct caaactggaa
    45301 gaagcacaat agaaccatcg atcatctgga gtctttatga aactttttcg tatcctcgat
    45361 cctttcacct taaccctgat cacggtggtg ttgctggcct ctttctttcc ggccagaggc
    45421 gatttcgtcc ccttctttga aaatctgacc accgcagcta ttgccctgct gttctttatg
    45481 cacggcgcga agttgtcgcg tgaggcgatt attgctggcg gtggtcactg gcgactgcat
    45541 ttgtgggtaa tgtgcagcac cttcgtgctg tttccgattc tgggtgtact gtttgcctgg
    45601 tggaaaccgg taaatgtcga cccgatgctc tactccggtt ttctctactt gtgcattctc
    45661 ccggctaccg tgcagtctgc aatcgccttc acgtcaatgg cgggcggtaa cgtcgcggcg
    45721 gcggtttgtt ctgcgtcagc atccagcctg ctggggattt tcctttcacc attgctggtt
    45781 ggtctggtga tgaatgttca cggtgcaagg ggcagccttg agcaggtcgg taaaattatg
    45841 ctgcaactgc tgctgccgtt tgtgttgggg catctttccc ggccgtggat tggtgactgg
    45901 gtgtcgcgca ataaaaaatg gattgcgaaa actgaccaga cgtccattct gttggtggtt
    45961 tatacagcgt tcagcgaagc cgtcgttaat ggtatctggc ataaagttgg ctggggatca
    46021 ttgctgttta tcgtggtggt cagctgcgtt cttctggcta tcgtgattgt agttaacgtc
    46081 tttatggcac gccgactgag cttcaataag gcagatgaaa ttactatcgt cttttgtggt
    46141 tcgaaaaaga gtctggcaaa tggcatcccg atggcaaaca ttctgttccc cacatcggtg
    46201 atcggtatga tggtgctgcc cctgatgatt ttccatcaga tccaattgat ggtctgtgcg
    46261 gtgctggcgc gtcgatacaa acgccagacc gaacagttac aggcgcagca ggaaagcagc
    46321 gccgataaag cttaaagcgg acgcttcagg ggctggacca gttgcgtcag cccctcggtt
    46381 ttaatcagca gcgtgatttg cattagctca ccgagcttcc ctgccgggaa ctcatctttg
    46441 cgggcaaacc acagcagata ctcctccggc aggtcaatta agcgacgccc tttgtatttg
    46501 ccaaacggca ttatcgtatt ggctatttca atcagctgct ctttttccat ctcagctacc
    46561 cagcaaacgc agcatttccg cttcgtcgat gacttcaatg cccagttcct gcgccttcgc
    46621 cagtttagat cctgcagctt cacccgctat caccagatcg gttttcttcg acacgctgcc
    46681 cgcgactttc gcgcccagtt cgaccagtcg agctttagcg tcatcacgcg acatctggct
    46741 taagctgccc gtaagcacca cggttttacc agcaaacggg ctgtcaatct cttccgcgtt
    46801 gataacgatc ggcgcaggcc agtgaacacc ttccgccaac agctcgctga tgacattgcg
    46861 gttgctttct tcggcaaaga agttgtgaac gtgggatgca acgacaatgc caacatcagg
    46921 caccttttgc agctcttcaa tcgaagcggc ttccagcgct tccagcgtgc cgaaatatgc
    46981 cgccagacct gctgcggtgg cctcgccgac ttcacggatg ccaagtgcat agaggaagcg
    47041 agcaaaggtg gtttctttcg ctttttccag cgcgttaacc acgttttgtg ccaattttgg
    47101 ccccatacgc tccagtccgg tcagtttgcc tgcggtgagt ttgaacagat ctgccggagt
    47161 atggacatat tctttttcaa ccagctgatc gatgattttg tcgcccattc cgtcaacatc
    47221 catcgcacgg cgggaaacaa agtgtttcag cgactcttta cgctgcgcac cgcaaatcag
    47281 gccaccggta cagcgggcaa ccgcttcacc ttccacacgc tcaacgtcag aaccacatac
    47341 cggacaatgc gtcgggaata caacctcacg ggtatcttcc gggcgttcag aaagcacgac
    47401 gttaaccacc tgcgggatca cgtcgccagc gcggcgaatc accactttat cgccaatgcg
    47461 taaaccaaga cgttcgattt catccgcatt gtgtaaggtt gcgttactca ccagcacgcc
    47521 agcaacatgg acaggttcca gacgcgcaac aggcgtaatc gcgccagtac gcccaacctg
    47581 aaactcgacg tcacgcacaa aggtcatctg ctcctgcgcc gggaatttaa acgcaaccgc
    47641 ccagcgcggg gcacgcgcga caaagccaag ctgctcctgc tgttccagtg agttgacctt
    47701 aatcaccacg ccgtcgatat caaagcccag cgtcgggcgg tcttcttcca ctttgtgata
    47761 gaacgccagc acttcttccg ccgattcaca aagcgttacc cgatcgctga ccggcacccc
    47821 ccacttttta aattgcagta aacggccaag atgagtatcc ggcagctcgc caccttccag
    47881 aacaccaacg ccatagcaga aaaaagtgag cggtcgcttc gctgtaatac gcggatcaag
    47941 ctgacgcagt gaaccagctg ccgcattacg tgggttagca aaaactttcc cgcccgtgcg
    48001 tcgcgcatct tcgttaatct tttcgaaccc cgcctgcggc aggaacactt caccacgcac
    48061 ttccagacgc gccgggatat tctctccgtg cagcttcagc ggaatggcgc gaatagtacg
    48121 cacattagac gtgatatctt ccccggtggt gccatcgcca cgggtcgcgg cactgactaa
    48181 aacgccattt tcatacagaa tactgacggc aaggccatcc agcttcagct cacagcacca
    48241 ggtgactttc tcgttgtttt tcagacggtc ctgcacacgt ttgttgaaag caagaaagct
    48301 ttcttcatca aaaacgttat ccagtgacag cattggtact tcatggcgta tctggctgaa
    48361 agctgccagc ggcgcagcac ctacacgttg ggtaggcgaa tcaggcgtaa tcagttctgg
    48421 atgtttggtt tccagctcgc gcagttcgcg catcagcctg tcgtattcag cgtcgggaat
    48481 ttccggcgca tccatcacat gataaagata ttcatgatgg caaagcgtcg ttcgcagttc
    48541 tgtcagttgt tgttcgattg attccatatc gcaccatcaa tgctaaaaac ccccgacaag
    48601 cgggggttcg aagaggagtt aatttgcctt aagtgtatca ggcgttggcg tctttgactt
    48661 cgcggatgat gtcctggtac tcgcgcaatt tctgcggagt catcatacgg cgctgatcgt
    48721 caagcacgac accgcccact tcatcggcaa tatgctgcgc agattgcagc atcagcttga
    48781 agttctgcag ctcgtcaccg taagacggta cctgcataaa gatagtgaca cccggagtag
    48841 tgaaatcctt catttcagga tcaaaggttc ccggtttcac catattcgcc aggctgaata
    48901 acgccgggcc gctgccatcc gggctaagat gacgatggta aatattcata tcgccaaaaa
    48961 tgaagcccgc ttgttgaatg ctgttaagaa gcagttcacc gtttagctcg ctaccgtgat
    49021 gcgccgcgac gttcatgata atcaccgctt ctttgcgctt cggtttatcc ataactggag
    49081 ccggttccgc tacaggctca ggctgtggtg ccgctacggg ttctgcaggc tggaaagcct
    49141 gttgtgccgg ttgcggtgct gaatgcacag gctgcggcgc tggcgcgacc tgtggcgaaa
    49201 ctggctgctg caacggctgt tcaggctgcg gctgataggc aggctgctgc accggctgcg
    49261 ctggacgcgg agcatgttgc ggcggtacct gcgcttcagg cggctgctgg accggttgac
    49321 gcggctgcgc agacgcataa ggcggtaggt actggtgttg cggcgacgga cgagcagcct
    49381 catgctcctg agcgttagcc ggggcatgat tcacgcggtg aacacgaacc tcaccaacgc
    49441 cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac ttcattcgtt
    49501 ttaatggccg atcgcggaac atagaagatc gttctttacg gctggtccag aaaccatgta
    49561 ccagtaaagc gattatggcg atcgcgccaa caatgattaa tatcagacgc aaatcctgca
    49621 tcattatatt ctctgttgtt ctaacacctt gccaccacgg caaacattta ctcactaaga
    49681 gtatttgccg attacctcaa gtgcaagtgc actattaact ttcacagcac aaagatagat
    49741 gaaatcgtgc tttttgctgt tttttcgaac atatcctaac tgtccattgc gcaattaccc
    49801 ggtaaaatac gcagaatttt cctgggattg gtcaaaagga gctcatcctg attatggttt
    49861 catcattcac atctgcccca cgcagcggtt tttactattt tgcgcaaggc tggaagctcg
    49921 tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct gctggtcaat attttgttga
    49981 tggggggcgc attctggtgg ctctttacac agctcgatgt ctggatcccg actttcatga
    50041 gttacgttcc ggactggcta caatggctga gttatctgtt gtggcctctg gctgtcatct
    50101 ctgtgctgtt agtgtttggc tatttcttct ccacgattgc taactggatt gccgctccgt
    50161 ttaacggttt attggctgaa caactggaag cacgattgac tggcgctaca ccgccagata
    50221 ccgggatttt cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa tggcaaaaat
    50281 ttgtctggta tctgccgcgc gcaattgtat tactaattct ttacttcatc cctggtattg
    50341 ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta gccatccagt
    50401 attgcgatta ccccttcgat aaccacaaag tgccgtttaa agagatgcgc accgccctgc
    50461 gcacacgcaa aatcaccaat atgcagtttg gtgctttaac cagcctgttt acgatgatcc
    50521 cgctgcttaa tctgttcatc atgcccgttg ccgtttgtgg cgcgacggcg atgtgggtcg
    50581 attgctatcg cgataaacac gcgatgtggc ggtaacaatc taccggttat tttgtaaacc
    50641 gtttgtgtga aacaggggtg gcttatgccg ccccttattc catcttgcat gtcattattt
    50701 cccttctgta tatagatatg ctaaatcctt acttccgcat attctctgag cgggtatgct
    50761 acctgttgta tcccaatttc atacagttaa ggacaggcca tgagtaagat ttttgaagat
    50821 aactcgctga ctatcggtca cacgccgctg gttcgcctga atcgcatcgg taacggacgc
    50881 attctggcga aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg tatcggtgcc
    50941 aacatgattt gggatgccga aaagcgcggc gtgctgaaac caggcgttga actggttgaa
    51001 ccgaccagcg gtaataccgg gattgcactg gcctatgtag ctgccgctcg cggttacaaa
    51061 ctcaccctga ccatgccaga aaccatgagt attgaacgcc gcaagctgct gaaagcgtta
    51121 ggtgcaaacc tggtgctgac ggaaggtgct aaaggcatga aaggcgcaat ccaaaaagca
    51181 gaagaaattg tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt cagcaatccg
    51241 gcaaaccctg aaattcacga aaaaaccacc ggtccggaaa tctgggaaga taccgacggt
    51301 caggttgatg tatttattgc tggcgttggg actggcggta cgctgactgg cgtcagccgc
    51361 tacattaaag gcaccaaagg caagaccgat cttatctctg tcgccgttga gccaaccgat
    51421 tctccagtta tcgcccaggt gctggcaggt gaagagatta aacctggccc gcataaaatt
    51481 cagggtattg gcgctggttt tatcccggct aacctcgatc tcaagctggt cgataaagtc
    51541 attggcatca ccaatgaaga agccatttct accgcccgtc gtctaatgga agaagaaggt
    51601 attcttgcag gtatctcttc tggagcagct gttgccgcag cgttgaaact acaagaagat
    51661 gaaagcttta ccaacaagaa tattgtggtt attctaccat catcgggtga gcgttattta
    51721 agcaccgcat tgtttgccga tctcttcact gagaaagaat tgcaacagta atgccagctt
    51781 gttaaaaatg cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc ttcaaacttt
    51841 cgcccctcct ggcattgatt cagcctgtcg gaactggtat ttaaccagac taattatttt
    51901 gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga atcgatttta tgatttggtt
    51961 caattcttcc tttagcggca taatgcttaa tgacgtacga aacgtcagcg gtcaacaccc
    52021 gccagcaatg gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac tggcgctaac
    52081 aatacaggct aaagtcgaac cgccaggcta gactttagtt ccacaacact aaacctataa
    52141 gttggggaaa tacaatgttc cagcaagaag ttaccattac cgctccgaac ggtctgcaca
    52201 cccgccctgc tgcccagttt gtaaaagaag ctaagggctt cacttctgaa attactgtga
    52261 cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact ctgggcctga
    52321 ctcaaggtac cgttgtgact atctccgcag aaggcgaaga cgagcagaaa gcggttgaac
    52381 atctggttaa actgatggcg gaactcgagt aatttcccgg gttcttttaa aaatcagtca
    52441 caagtaaggt agggttatga tttcaggcat tttagcatcc ccgggtatcg ctttcggtaa
    52501 agctctgctt ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt ctgccgacca
    52561 ggttgatcag gaagttgaac gttttctgag cggtcgtgcc aaggcatcag cccagctgga
    52621 aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca tctttgaagg
    52681 gcatattatg ctgctcgaag atgaggagct ggagcaggaa atcatagccc tgattaaaga
    52741 taaacacatg acagctgacg cagctgctca tgaagttatc gaaggtcagg cttctgccct
    52801 ggaagagctg gatgatgaat acctgaaaga acgtgcggct gacgtacgtg atatcggtaa
    52861 gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca ttcaggatga
    52921 agtcattctg gttgccgctg acctgacgcc gtccgaaacc gcacagctga acctgaagaa
    52981 ggtgctgggt ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta tcatggcgcg
    53041 ttctctggaa ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg tgaaaaatga
    53101 cgactatctg attctggatg ccgtaaataa tcaggtttac gtcaatccaa ccaacgaagt
    53161 tattgataaa atgcgcgctg ttcaggagca agtggcttct gaaaaagcag agcttgctaa
    53221 actgaaagat ctgccagcta ttacgctgga cggtcaccag gtagaagtat gcgctaacat
    53281 tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg ttggtctgta
    53341 tcgtactgag ttcctgttca tggaccgcga cgcgctgccc actgaagaag aacagtttgc
    53401 tgcttacaaa gcagtggctg aagcgtgtgg ctcgcaggcg gttatcgttc gtaccatgga
    53461 catcggcggc gacaaagagc tgccatacat gaacttcccg aaagaagaga acccgttcct
    53521 cggctggcgc gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg atcagctccg
    53581 cgctatcctg cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga tgatcatctc
    53641 tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg aactgcgcga
    53701 cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta atggtggaaa caccggctgc
    53761 cgcaacaatt gcacgtcatt tagccaaaga agttgatttc tttagtatcg gcaccaatga
    53821 tttaacgcag tacactctgg cagttgaccg tggtaatgat atgatttcac acctttacca
    53881 gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt attgatgctt ctcatgctga
    53941 aggcaaatgg actggcatgt gtggtgagct tgctggcgat gaacgtgcta cacttctgtt
    54001 gctggggatg ggtctggacg aattctctat gagcgccatt tctatcccgc gcattaagaa
    54061 gattatccgt aacacgaact tcgaagatgc gaaggtgtta gcagagcagg ctcttgctca
    54121 accgacaacg gacgagttaa tgacgctggt taacaagttc attgaagaaa aaacaatctg
    54181 ctaatccacg agatgcggcc caatttactg cttaggagaa gatcatgggt ttgttcgata
    54241 aactgaaatc tctggtttcc gacgacaaaa aggataccgg aactattgag atcattgctc
    54301 cgctctctgg cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt gcggaaaaaa
    54361 tcgttggtga tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg cctgtagacg
    54421 gtaccattgg taaaatcttt gaaaccaatc acgcgttctc tatcgaatct gatagcggcg
    54481 ttgaactgtt cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa ggcttcaagc
    54541 gtattgctga agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa tttgatctgc
    54601 cgctgctgga agagaaagcc aagtctaccc tgactccggt tgttatctcc aacatggacg
    54661 aaatcaaaga actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc ccggttatcc
    54721 gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc catcggcgcc attttttatg
    54781 cttccgccag cggcggcaaa atcaattcat cgctctcatg ctgctgggtg tagcgcatca
    54841 cttccagtac gcgcaacccc gcacggtgca ccgcatcgtt taacgccttc cctttcagca
    54901 aaccactgat aagctgagca caaaacaggt cgccagtccc tttcaggtcg gtttttaccc
    54961 gtgaatggga aatgacattc acgctgtcgg cactgaccac caccacctgc atctcctgat
    55021 tttcttcatt accggaggcg ctggtaatca ccacccattt taatgtgtct gaaagcagac
    55081 tttttgcggc agcaatggca ctgtcgagat cgcggcaatt tttaccggtc aggatttcca
    55141 gctcaaagat attgggggta attccctgcg ccagcggcag taaatattgt cgatacgcct
    55201 cgggaaggtc aggtttgaca taaattccgc tatcaatatc gccaatcacc ggatcgacca
    55261 tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag ccactcggca aggattttga
    55321 tttgcgatgc cgttcccata tagcccgtgg ttacagcacg aagttggcgc agcgcatcac
    55381 gctcctgaag cgcacgcaaa tagccgctaa accattcgtc cggaatcgca ccaccgtaga
    55441 aagtgtcata atgcggcgta ttgctcagca ataccgtcgg cacggcaaag acattcaagc
    55501 cgttctgttt tatagcaggc accgcaatgc tgttgcctac gcttccgtac actacctgcg
    55561 actgcacggc gacgatatcc gcctgcagcg ccctactctt atcgttaaac aacaacaaac
    55621 tactcattta attttttctc cttgccgatg atcctcatcg tattccaacc gaaactttac
    55681 ctgattctgg cagtcaaatc ggctattaca aaacaaagat aaggtaattc agtgaagaaa
    55741 atcatttgtc tggccattac gctgctaatg acactccccg cttacgcgaa gttaactgtt
    55801 catgaagaag cccgcatcaa cgccatgctg gagggattag cacagaaaaa ggatttgata
    55861 tttgtgcgca acggtgatga acatacctgc gatgaagcgg tttcccacct gcgtctgaag
    55921 ctcggcaata cccgtaaccg cattgacact gccgagcagt ttattgataa ggtagcttcg
    55981 tcgtcatcga ttaccgggaa gccgtatatc gtgaagatcc ccggtaagag cgatgagaac
    56041 gcacagcctt ttttacatgc gttaattgcg cagacggata aaacggtgcc tgcggaagga
    56101 aattaatccg ctttggtaag gcatttacag gaggtaacat gaaaaaatgc tttatttatc
    56161 acgatgaaaa atcgaataaa ttttggtgga tagattacga aggggatagt ttagctgtca
    56221 actatggcaa ggtaggtagt attggtaaat tccatacaaa agaattcgat aatgaagaac
    56281 agtgtctgaa agaagccagt aaattgattg ccgcaaaaat gaaaaaagga tatcaagaaa
    56341 ctccagactt taacttcatg gatctctact attttgatga tgaagagatt gggttacatc
    56401 ttaaaacgtc acacccaaac ttccagtgcc attttactga tccactttat atgtgttgct
    56461 gggatgaaga atctcctttt ggtagcgatg aaggtgctga tgctctaaac gaacttgaaa
    56521 atagcctccg ttctggactg tgctgatttc cctcaaatgt taattgaaac tatgtggggt
    56581 atgaaataca tcgctatgga cagtattctt gaagaggatg tgcgtgcgca attactagcc
    56641 gatgaaatga gctctatcca gagcaatatg attacctacg cgactgcatt cggtcagatc
    56701 aaagtcatgg gtaaaatctc ccataaactt aaaaagatgg gactcaatgc actagcgcgt
    56761 catcagctta ccgcaaaaat tcttcaatgg ggtgacggtc aggactcacc aatacttcaa
    56821 aaaatgattg atgaccttac tgcttttcca cacgaaaatt aaaaactgca tttgtcggca
    56881 gcaacaactg ccgacaaatc agattattga caaagtgcac tttgtttatg ccggatgcgg
    56941 cgtaaacgcc tgatccggca taaaaatatt gctaattcaa tatattgcag aaacattgta
    57001 ggcctgataa gcggagcgca tcaggcagtt ttgcgtttgt catcagtctc caatgctatt
    57061 aatccttaaa tccctgcccc ctgactaaaa tgctcttccc caaacactcc ggtggagagg
    57121 tagcgatcgc cacgatcgca gatgatcgct accaccaccg caccagggtt agcttttgcc
    57181 acccgcagtg ctccagcaac cgcgccgcct gagctgacgc cacagaatat tccttcccgc
    57241 accgccagtt cgcgcatggt gttttccgca tcgcgctgat gaatatccag cacctcatcc
    57301 accagcgcag cgttgaaaat ccccggcaga tattccgcag gccagcggcg aatgcccgga
    57361 atactgctgc cttcttccgg ttgcaggcca acaatggtca ccggtttgga ttgttcgcgc
    57421 ataaagcatg aaacgccggt gatagtgccg gtcgtcccca tgctggagac aaaatgagtg
    57481 atgcgcccgc cggtttgctg ccagatttcc ggcccggtgg tggtgtaatg cgcataaggg
    57541 ttatcgggat tattgaactg atcgagcagc tttccttcgc cacgattcgc catctccagc
    57601 gccagatcgc gcgcaccttc catgccctgc tctttggtga caagaatcag ttccgcacca
    57661 taagcacgca tcgccgcacg gcgttcctgg ctcatgttgt cgggcatcag caatttcatg
    57721 cgatagcctt tcagcgcggc aatcattgcc agcgcaatgc cggtgttacc actggtggct
    57781 tcgattaaga catcacccgg tttaatttcc ccgcgctttt ccgcctcgac gatcatcgaa
    57841 agtgccgcac gatctttcac cgaacctgcc gggttattgc cttccagttt taaccacact
    57901 tcactgccgt tatccggccc cattcgctgc aacttcacca gaggcgtatt gcctattgtt
    57961 tgttctaatg tactcacaat ctctatccat acgtggtgtt gcctgatgcg acgcttgcgc
    58021 gtcttatcag gcctacaggt tacaaacctt gccattaaaa agcccggatc gcgggaagcg
    58081 cctccgggcg tttaacattc actcaaccta tcaggcgctt tgtgcgagag caagttcctc
    58141 atcgcgggtt tcaatacgct cgtcgccgtt atacagccgc gcatgttgca gaccaacgaa
    58201 taaacgctcg ccatgctgcg gggcatcgtc gccatgcatc acgaccgtca gcggttcgtt
    58261 gtaccacccc agcggctgca ccactaattg ggtgtagtga cctttcgggc tggcttccag
    58321 tacctgtacc ggcagcggcg aatcgagact ggtacggcgg ctgatatcca cttcccacgg
    58381 gcgcaggaag agatccaccg gcccctgata cgcaggtgtg tagcccagcg gccagcgatg
    58441 cgcgccaaca tggaactgcc cgccgcgaat ggttccctgc aggcggttca cttcgcccat
    58501 aaattcgagt acaaaacggg tcgctggttc gcgccatacc tgattcggcg catcagcctg
    58561 ttcaatattc ccctgactca tcaccactac acgatcggct acttcggtcg cctcttcctg
    58621 gtcgtgggtc acaaacacgc tggtgaattt cagctcttca tgcagttgac gcagccagcg
    58681 acgcagctct ttacgcacct gcgcatccag cgcgccgaac ggttcatcaa gcagcagaat
    58741 ttgcggttct acagccagcg cgcgcgccag cgccacacgc tgtttctggc cgccggaaag
    58801 ctgcgccgga taacgatccg ccagatgcgc aagctggacc atttccagca attttgtcac
    58861 tttcgctttg atggctgcgg cattcgggcg ctcgcgacgc ggcagcaccg tcaggccaaa
    58921 agtgatattg tcgaataccg tcatatggcg gaacagcgcg taatgctgga acacgaaacc
    58981 gactttacga tcgcgcgcgt gcaggcgact cacgtcggtg ccgtggaagc gaatatgccc
    59041 gctggtttga tgctccagcc cggcgataat ccgcagcagc gtggttttcc cggaaccgga
    59101 cggccccagc aacgcgacca tctgaccaga aggaatatcc agtgagatat cgttcagcac
    59161 ctgggtgcga ccaaaagact tcttgatatt ggcaatctca atgctcatga tgctcctcct
    59221 gctgcgcgcg tttttcctga ttctccaggc gccactgcaa catactcttt aaaaacaggg
    59281 tgataatcgc catcaacgtc aacagcgcgg cggcggtaaa ggagccgacg gtgttgtagt
    59341 cctgctccaa caattcaatc tgtaacggca gcgaaagtgt ttcgccgcga atcgagccgg
    59401 aaaccaccga cactgcacca aactcgccaa tcgcgcgggc gttggtcagc accacgccgt
    59461 aaagcagcgc ccagcgaata ttcggcaatg tgacgcgacg gaacatctgc cagccggacg
    59521 cgccaagcaa aatcgccgct tcgtcttcct ggctgccctg gcttaacatc accggcacca
    59581 gctcgcgcac cacaaacgga cacgtcacaa agatggtgac cagcaccatt ccgggccagg
    59641 agaacataat ttgcagatta tgttcatcca gccagccgcc gagcgggccg ttggagccgt
    59701 agaacagcag atacaccaga ccggcaacca ccggcgatac agcaaacgga atgtccagta
    59761 gcgtcagcag taactggcgt cccgggaagt taaagcgcgt caccagccag gccagcagaa
    59821 tgccgaacac caggtttacc ggtacggcaa tcagcgcgat catcaccgtc agccagatgg
    59881 cgtgcagcat gtccggatcg gccagattct gtaaaaccgg catcagcccc ttgctgaatg
    59941 cctgcacgaa gatgtaaatc atcggcacca gcaggatgaa cgccgaaacc agcatcccga
    60001 tgccaatcag aaaccatttg ccccagttaa tcgggcgcgc gtcataacgc ttcaattggg
    60061 taacttccgc cattaatgac ctaccacacg ccgaccaaag cgactttgca gagtgttaat
    60121 tgagaacagc agcagcagag atgccgcgag gatcaccgaa gcaatcgcgc tcgctgccgg
    60181 gtaatcaaac tcctgtaagc gcacaaaaat catcagcgac gtcacttccg tcttccacgc
    60241 gatatttccg gcgataaaaa tcaccgcgcc aaattcacca agactacggg taaacgacag
    60301 cgccacgccc gccaccagcg ccggagaaag ctccggcagc accactttgc agaaactctg
    60361 ccagcgcgtt gcaccaagcg tttccgccgc ttcttcatat tccgggccta actcttccag
    60421 caccggctgc acggtacgca ccacaaacgg aatgctggta aaggccatcg ccaccgcaat
    60481 accaagccag gtataggtga ctttgatatc aaacttcgcc agccattcac cgtaaaaacc
    60541 gtttacggaa aagagcgagg ccagcgttaa accggcgact gccgttggca gcgcaaaggg
    60601 taaatccatc agcgcatcaa gcagcgtgcg gcccggaaag cgataacgtg ttaaaatcca
    60661 cgccatcagc agaccgaaaa cgccgttaaa aatcgatgcc acaaacgccg acagcagcgt
    60721 cactttgtag gccgcgacca cctgcgggtt ggtgatcacc tcccagtact gcgcccagct
    60781 catctcagaa agctgcatca ccagcgcaga gagcggcagc agcaatatca ggcacacaaa
    60841 cagcagactg gtgccaaggc ttaaagtaaa gcccggcagc acgcgtcttg aagaaactgc
    60901 aaacattact tacgccccgc cgctaacagc ttgtctaact cgccgccgct ggtgaagtgg
    60961 gttttcatca cttccggcca tgagccaaat ttgtcttcca cgcggaacag ttcggtctgc
    61021 gggaatttat ctttcagttt gtccatgact tccgggttat tcacacggta gtaatagtcg
    61081 gtgatgatgg tctgcgcctg tgggctgtag agccagttca ggtaggcttt tgcggctttt
    61141 tccgtaccgt tggcctgcac atttttatcg acccacgcca ccgggaattc cgccagaata
    61201 ttggttttcg ggatcaccac ttcaaagccc tgtgcttcat actgtttacg gatgttgttc
    61261 acttccgatt caaagctaat cagcacatcg cccaagccac gctcggcaaa agtggtggtt
    61321 gcgccacgac cgccggtatc gaacacttca acgtttttca ggaactgggt cataaactgt
    61381 tcggttttgg ctttatcgcc accgtcagct ttatccgctg cgccccatgc cgccagatag
    61441 gtataacgcg cgttacccga cgttttcggg ttcgggaaaa tcagcttcac gtcggagcgc
    61501 accaggtcgt tccagtcgtg gatattcttc gggttgccct tacgcaccag gaagcccatg
    61561 gtggagtaga acggcgagct attattcggc aggcgcgact gccagtcagc cgggatcagc
    61621 ttgcctttgt cgtgcaggat ttgtacgtcg gtcacctggt tataagtgac aacgtctgct
    61681 tttaagccct gcaaaatcgc cagcgcctgt tttgatgacc cggcatgaga ttgttttatc
    61741 gtcagtttgt cgccgccgtt atcttttgcc cattgctgtt caaacggcgg attcagggcg
    61801 gcaaacagct cgcgggagac gtcataagaa ctgttcagca gttccgttgc ctgtacatgg
    61861 cccgccagca gcagagaagc gaccagcgcg agtgagttct ttttcagtaa gttaacggcc
    61921 attgcgcacc cttataaatt taatgacttt ctaatagcca tcatatttat aacggacgtg
    61981 aaaggagtaa cggttttata taccgtttgg tgatttggaa gttaaaaagg gaataagaac
    62041 ttggttcaac aggcgtaatt gttgcaggca gtttggacac ggacagcgcg gagaaaccgg
    62101 agcgtacaca tagtacgtga ggatttcgag cactgcccgg ggccaaaatg acaaatgaaa
    62161 tagcctggtg aacttagttc aagacgaaat cctccccaca catgcaggga ggaaacagag
    62221 gtgaatcaga taccgacgct aaccgtctcc ggcagtgtgc tgccgccatc aatcacattc
    62281 tgtgtaccgg ttaaatagct ggattcatcc gatgcgagga aggccgccag ttcgccgact
    62341 tccagcggat cggcgaggcg acacagcggg attgcttttg ccatttcagt cagcaccgat
    62401 tctggatctt ccgggttcga ctggcgggca atgctttccg ccattggcgt gcggacgtat
    62461 cccgggcaga tggcgttaac gcgaataccg gattgcgcgt actccaccgc cagcgattta
    62521 gtcaggccaa caatcgccgc tttcgttaag gcatacgccg tttcgccagg atcggccacc
    62581 atatcaccag tgactgaaga catcatcaca atgcgaccat ctttgcgcgc aatcatctcc
    62641 ggcagcaccg ccttcgtgac gttccatacg cctttaatat tgatatcaat atggaaatcg
    62701 cgatcttcat cgctcatatc gaggaaactg cccagacgac aaacgcctgc gttattcacc
    62761 aggatatcaa tgcgcccttc tttttccttc gcgcgtttga tagctgcggc taccgacgcc
    62821 gggtcacgca catcggcgac aaccgccgta cagcgatgac cacgaccaca cagttcgtcc
    62881 gctagctttt cgatctcagg ggagatatcc agcaagatta ggttcgcgcc atgacgtgca
    62941 aaagttctgg caattccttc gccaattccc tgcaatgcgc ccgtaatcag tgctgtcttg
    63001 cccgtgagtt tacccatttt aatgctctcc ttgttaatac agagcgttac actccattcc
    63061 ctctgaaaat acaggataaa caaaatattg ctgcggatca gctcactaat tcaggaaaat
    63121 ctcaatccag tcgctgcttt atgcatcatt tcaggcgttg tgttagctca ctgctgcgct
    63181 gaatcatttt caatgacgcc acatcattga tgttgcacaa gaccaacaaa cagtaaatcg
    63241 gtaacagaat tttgcgccgt gcgggtagac atcgacgagc tacgccattc ggtttcaccg
    63301 gaaacggtat cgagcgtgaa atgcgccagc cgccgcagcg gagagtcagc cagagaagta
    63361 atggcaatca ccgttgcccc ctgctttcgt gccgcttctg cgcacaagac aatctctttt
    63421 ttactgccgc tgtaagagat agcgatttgt acatcgcctt ttttcaacgc ctgggaaacg
    63481 gtggcttgta cgtgcgtgtc ggcttcgcag gccacgcgat agccaatttt catcagtttg
    63541 aacgacagat cgcgccccac cagcgccgat ccgcccaggc ccgttatctg gataaacggt
    63601 gctttactga tgacctcaat gattttttgc agccgcgcgt agtcgaacaa cgcacaggtt
    63661 tgctccagcg ccagctcttt ttcgcgattc agtttgcggg caatcacttc cagcgaatca
    63721 tcactggtaa tcgaactgtg caggtgcagc gccgtggcat tcgttttctc ccggctggca
    63781 ctgtattcgc caattaacgc catacgcaat tcagtaaatc cttgcgcgcc gagcttctgg
    63841 gcaaatttca caatgctcga ctggctgata ccgagctgtt tcgccatctg ccgtgaagag
    63901 acggatttca gctcgctcac tctggcccgc agaaagtcag caattttttg ctcgttcccg
    63961 gtaaattcac tttccgcatt gcgtattttc gtcaggtaca acatcttcca ctctcctggg
    64021 ctggcagcaa aaattccgct taagttctta tgacgctctt cacactctgc gagtgtagac
    64081 tcaatgattc ctttaagtct gtcaattcgg gatatatgat tccatacatc tgcaattatt
    64141 tgatcgtaaa tagtaaggtc accaccgatg caactagaaa agatgattac tgaaggctcg
    64201 aacaccgcct cggctgaaat tgaccgcgta tcgacgctgg aaatgtgccg gattatcaac
    64261 gatgaagata aaaccgtacc gcctgccgtt gatcgcgtcc tgccggatat cgccgcggcg
    64321 atcgatgtta tccacgccca ggtcagcggc ggcggtcgtc tgatttatct cggtgcggga
    64381 acatccggtc gtctggggat tctggatgcc agcgaatgtc cgcccaccta cggcgtgaaa
    64441 ccgggtctgg tggttggttt gattgctgga ggcgaatatg ccattcagca cgcggtggaa
    64501 ggagctgaag atagccggga aggtggtgtt aacgatctga aaaatattaa tttaacggca
    64561 caggatgtgg tggttggcat tgctgccagc ggtcgcacgc cgtatgtgat tgccggactg
    64621 gaatacgcac gccagctcgg ctgccgcaca gtgggaattt cctgtaatcc ggggagcgcc
    64681 gtttcaacca ccgctgagtt tgccattaca ccgattgtag gtgccgaagt tgttaccggt
    64741 tcttcgcgga tgaaagcagg tacagcgcag aaactggtgc tcaatatgct ttccaccggg
    64801 ctgatgatta aatccggcaa agtgttcggc aacctgatgg tcgatgtggt cgccaccaac
    64861 gaaaaactgc atgtgcgcca ggtcaatatc gttaaaaacg ccaccggatg taatgccgaa
    64921 caagcggaag cggcgttaat tgcctgcgag cgcaactgta aaacggccat tgtgatggtg
    64981 ctgaaaaatc tcgatgccgc agaagctaaa aaacgcctgg atcaacacgg cggctttatt
    65041 cgtcaggttt tagacaagga ataacccatg gccaaagaga tcagcagtga acttctgaac
    65101 accattctta cccgtgtcgg cggaccggga aatatcgcca gttgtggtaa ctgtatgacg
    65161 cgcctgcgtc tgggtgtaca tgacagttca ctggttgatc ccaatatcaa aacgctggaa
    65221 ggcgtgaagg gcgtcatttt gaccagcgac caggtgcagg tcgtttttgg gcctggtaaa
    65281 gcgcatcgtg ccgcaaaagc gatgagcgag ctgctgggcg atgcaccggt acaggatgcc
    65341 gcagaaatcg ccgcccagaa caaacgtcag ttaaaagcca aacaaacctc cggcgtgcaa
    65401 caatttctcg ccaaattcgc caccatcttc acgccgctga tccccggttt tattgccgcc
    65461 ggtctgctgc tggggatagc gacgttaatt gccacggtga tgcacgttcc ggcagatgct
    65521 cagggcacgc tacccgacgc gctgaatttt atgaaggtgt tcagcaaagg tttgttcacc
    65581 ttcctagtga ttctggtggg ctataacgcc gcccaggcat ttggcggcac gggcgtcaat
    65641 ggcgcaatta ttgccgcact ctttttgctc ggttataacc ccgctgcaac caccggttac
    65701 tacgccggtt ttcacgattt ctttggtctg cccatcgatc cgcgcggcaa tattatcggc
    65761 gtgctgattg ccgcgtgggc ctgcgcccgc attgaaggca tggtgcgccg ctttatgccg
    65821 gacgatctcg acatgctgct gacctcgtta atcaccctgc tgatcaccgc tacgcttgcg
    65881 tacctgatca ttatgccgct gggtggctgg ttattcgaag gtatgtcatg gctgtttatg
    65941 cacctgaaca gtaatccgtt cggttgtgcg gttttagccg ggctgttcct gatcgccgtg
    66001 gtatttggcg tgcatcaggg ctttattcct gtttacctcg cgttaatgga cagccaggga
    66061 ttcaacagct tatttcccat cctttcaatg gcaggcgcgg gccaggtggg cgcggcgctg
    66121 gcactctact ggcgggcgca accgcacagt gcgctacgca gtcaggtacg cggggcgatt
    66181 attcccggcc tgctgggcgt tggtgaaccg ctgatttacg gtgtcaccct gccccgcatg
    66241 aagccgtttg ttaccgcctg tttaggcggc gcggcgggcg gtttgtttat cggattgata
    66301 gcctggtggg gtctgccgat gggcttaaac agcgcctttg ggccgtctgg tctggtggcg
    66361 ctgccgctga tgacttccgc acaaggcatc cttccggcaa tggcggttta tgctggcggg
    66421 attctggtgg catgggtttg cgggtttatt ttcaccacgc tctttggctg ccgtaacgtc
    66481 aatctggact gatattatga aacggacaat gctctatctt tctctgctgg ctgttagttg
    66541 tagcgtcagc gccgcaaaat accctgttct gacagaaggt agttgatcaa gtatatgcgg
    66601 cgttaaagca gtaataaaaa ggccagtcag taatgttact gcctggcctt taatcaatga
    66661 atcagaaacg cttacagcgc catcaacttg tccagcgacg gtgcgaaata atagccgccg
    66721 gttaccggtt tggtgaaacg caacatcgca tcacgtttgc catcggtatc gccaaacatg
    66781 ctcagcagtt gctgctcaat gttatgcaga cgcgcgcagt aggcgcagaa gtacagaccg
    66841 tgagtgccac tggcggtgcc gtacggcagg ctctggcgaa caatcttcag ccctttgcca
    66901 tcttctttca gatcaacgcg ggtgaggtga gaggtttccg gacgttcgtc gccgtcaatc
    66961 tcttcgttgg cctctttggt gcgcccgatc atcatctcct gatcgtgaac gctcatccgg
    67021 ttgagctgct tcaggttgtg ctcccaacgc tgcacaaaca catagctgcc gccggaatcc
    67081 acgccgtctt tgataaccgc cacttcgcgg cgcgtctctt cacccgccgg gttttctgta
    67141 ccgtcaacaa agccgctcag gtcacgctct tccacccaac ggaagccgtg gatctcttct
    67201 ttcacttcaa tgcagtcacc aaaggcttcc atcgccgcct gagcgacaga gaagtttacg
    67261 tcgtgacgca gagaaagaat gtggatcaac acatcgaact gggtcgtcgg cgcaaggcct
    67321 ttaccgtagc ccggaaaatc tttcagctct tctgccccaa cgccgccgct cagagcgcgc
    67381 caggtgttgt taccaaaggc aaccaccgca ccaagatgcg cgtccgggaa tttcgcttca
    67441 aaagttgcca gtttgtcggc aaatgtttta ctggccgcac gcagggcgtc aacttcccct
    67501 ttcacgttgg cttcgatcca aatcgccgcg cggcaatgtt ctggcaaaat gccactctga
    67561 acctgagaca ttgttcctcc tgaaaataag aatgccacgc aatccgtggc attgttgagc
    67621 gctattttac gtgttttttc gcgacggcgg tttgttcaga cgcaaattaa cgccgccaga
    67681 taattttact gactttccag tttttcaggg tatcgtccga aggcattaac ccttcaggac
    67741 cacgccattc cccgctgaac tggtagctaa tatgttgact gccttcggct ttacattcga
    67801 cggcacgatt atcatcacca tcagcttttt ggcaattgcc aaatgctttg ctgtaaaggt
    67861 cgctaaacgg tgtaccgatt ttaacaccgg tgtcagcagg aatatcgcta tccagcacgt
    67921 caatgcgact gatcgtgcct tgatcgccgt taatcaccat cgccacgttg tcgcctttca
    67981 tcacttcaaa aaagcgcacc acgttgccgt tcgcggtttt cattccgctg cgcaggcgat
    68041 aatcgccatc aagcgcatca gcaatggctt gttcttgcag tggtgtggac gccgttaatt
    68101 cacccacgcc ctgctcgctc actttggtgg acgatccaaa ccagttccac gggttagcgg
    68161 cagaccagtt aactgacgac atcgtggaac agccggtcag catcaacggc atagcgcata
    68221 acattaaacg cagcgatttc atgtcacttc ctttggttat tcaataacgt tgcttggagt
    68281 gcaaattcac caaaaagtgc cgttattctt tttcttcatt aaaacaagcg cgtaaacgtc
    68341 gattggtcag cagccagatt aacgccacaa tatccgccac taccagcgcc agaccgatac
    68401 cgctaacgga ttcaccgttc agccacagcc acggttgcca gcaaagtaaa accacctgcg
    68461 ccaacagcaa cagaaaatag agcacatgcc aggtgcgagg aaacgaagcc cgccgaccgc
    68521 tcaacaaaaa cgccagcacc gccggaatac cgggaatcag ccccagccag aaattatcgt
    68581 gatcgggata aaacagattt agcagcgcag taccctgctc acgcgacgca ccggcaatga
    68641 caaacagcac ccaggttcgc gcctgaagca atagcacaag ccagaagagc aagggtaaac
    68701 gcaggcgtcc gtgcgcatca taatggacag gatggaactc agtactcttc atcttcaatc
    68761 aaacgcttac ccagactcag cacgtcggcg tgttcatatc ccaggcgttc atacattccg
    68821 agcaccatgt cgttatcttc cggcacgttg atctgaattt tcgggcagcc gcgagcaatc
    68881 agctttttct ccagccggtt aagcaacgca ttggcaattc cacgcccacg aaactctgga
    68941 tgcacgccaa gataataagc agacccgcga tgcccgtcat aaccgcccat caccgttccg
    69001 accacttcac cgtttacctc agcgaccaaa aacaaactga cgtcatggtt catcttacgc
    69061 tcgatgtcca tttccggatc gttccacgga cgcagcaagt cgcaacgctc ccaaagggtg
    69121 atgacctctt cgaaatcttc ctggcgaaat acgcgtatct ccatggtatt cgttaccttt
    69181 ttgcgggtta aaaggctgat tatggcgtga acggtcgaat tagccaatat ctgacgaaaa
    69241 tcggttgaaa aagtggcata atggggagtt gtcaactatt gaaatgaaaa gtaaaacaat
    69301 tctcaacagc aaaccgtcgt aacggattac gcgatacgat ataacatctg gaactttatt
    69361 attacaactc aggccgtatg agcactttta aaccactaaa aacactcact tcgcgccgcc
    69421 aggtgctgaa agccggttta gctgccctga cgttgtcagg aatgtcgcaa gccattgcca
    69481 aagacgaacc tttaaaaacc agcaacggac acagcaagcc gaaagccaaa aaatctggcg
    69541 gcaaacgtgt cgttgttctc gatccaggcc acggcggaat tgataccgga gcgatcggac
    69601 gcaacggttc gaaagaaaaa catgtggtgc tggcgattgc taaaaacgtc cgttccattt
    69661 tgcgtaatca tgggattgat gcgcgtttaa cgcgttctgg cgatacgttt atcccacttt
    69721 acgatcgcgt tgaaatcgcc cataaacatg gcgcagatct gtttatgtca attcatgccg
    69781 atggctttac caacccgaaa gctgccggtg cttcggtatt tgccctctct aaccgtgggg
    69841 caagtagcgc aatggcgaaa tacctgtctg aacgcgaaaa ccgcgccgat gaagttgccg
    69901 gtaaaaaggc gactgacaag gatcacctat tgcagcaagt actgtttgat ctggtgcaaa
    69961 ccgatactat taaaaacagc ctgacgcttg gctcgcatat tctgaaaaag attaagccgg
    70021 tgcataaact gcacagccgc aacaccgaac aagccgcgtt tgtggtgttg aaatcgccgt
    70081 cggttccttc ggtgctggtg gaaacctcgt ttatcaccaa cccggaagaa gagcggctgt
    70141 taggcacggc ggcgtttcgt cagaaaatcg ccacagcgat tgctgaaggc gtgatcagtt
    70201 atttccactg gttcgacaac cagaaagcac attcgaaaaa gcgataagtt atgaaacccg
    70261 acgcacacca ggttaaacag tttctgctca accttcagga tacgatttgt cagcagctga
    70321 ccgccgtcga tggcgcagaa tttgtcgaag atagttggca gcgcgaagct ggcggcggcg
    70381 ggcgcagtcg ggtgttgcgt aatggtggtg ttttcgaaca ggcgggcgtc aacttttctc
    70441 acgtccacgg tgaggcgatg cctgcttccg ccaccgctca tcgcccggaa cttgccgggc
    70501 gcagtttcga ggcgatgggc gtttcactgg tagtgcatcc gcataacccg tatgttccca
    70561 ccagccacgc gaatgtgcgt ttttttattg ccgaaaaacc gggtgcagag cccgtttggt
    70621 ggtttggcgg cggcttcgat ttaacccctt tctatggttt tgaagaagac gccattcact
    70681 ggcaccgcac cgcccgtgac ctgtgccagc catttggtga agacgtttat ccccgttaca
    70741 aaaagtggtg cgacgattac ttctacctca aacatcgcaa cgaacagcgc ggcattggcg
    70801 ggctgttctt tgatgatctg aacacgccag atttcgacca ctgttttgcc tttatgcagg
    70861 cggtaggcaa aggctacacc gacgcttatt taccaattgt agagcgacgt aaagcgatgg
    70921 cctacggcga gcgcgagcgc aattttcagc tctaccgtcg cggtcgttat gtcgagttca
    70981 atctggtctg ggatcgcggc acgctgtttg gcctgcaaac tggcgggcgc accgagtcga
    71041 tcctgatgtc aatgccgcca ctggtacgct gggagtatga ttatcagcca aaagatggca
    71101 gcccagaagc ggcgttaagt gagtttatta aggtcaggga ttgggtgtaa ctccctcacc
    71161 cccattcccg catccgctga tgcaacgtca acgactgctt ctcggcaaac agctgctggt
    71221 aatccgtggc aaattgcccc agatgccaga atccccactg catggcggcg tcttttaccg
    71281 ttgtgctttg cgaccacggg cttatcagtt cgcggcgtac ggcgttcagg cgaatgcgtt
    71341 tcagccacgc gtttgggcca atgcctaaaa tagcgtgaaa cgcgttttgt agcgtgcggc
    71401 gactgacatg cagttgatta cacaaatcca gcaccgtcac cggttcggac atgttttcca
    71461 gcacatattc acgggcgcgg gaaagcaacc gacggtaact ctgatgactg atgctttccg
    71521 ccgtcaccat tggttgcgct tcttccagca tcgcccccat cgccattagc aaattatccc
    71581 ccagcacttt tcgcactgca ggctgatgga gattttccgg gttctcgcaa aacgtcgcca
    71641 gcgcctgttg gacaaagccc cacagcgcgg ctttatgctg ctctttcact tccagcgccg
    71701 actggctacg cagcatatgt aatacccgat ccggattgtg caaaaagtta gcctgacagg
    71761 tgatgacatc ttcagacagc actacgccca ggatcgtata atcgtccggc gtacttaatt
    71821 caaactcggt tccaccgggg cgcgtcgcta tttctgcgct tcccagacat tgcgaaccga
    71881 taaatccctg ctcaccgcgc gtcgccggaa tgccaaacca gaacgagttc ggccagacca
    71941 ggcacgactg acgcagcgcc agaccggtgt attcacgaaa aacctgaata tcatcgagta
    72001 gaatttccgt aaattcacca tgaaacttgc ccggatgcag ctgatcgtaa atctgctgct
    72061 aggcggtaat cgtcaaagca tgttcataga catccgttgt ctgtcgttga tgaacattat
    72121 ccacttcgac cttcggcgtg agcttcaggt tttcgggtaa gggttcatga taaagatggt
    72181 gcaaattggc tgtacgggtc tttttcatga tgttaatgcc gggtgttgta ggacacccgg
    72241 cacctccgac aggttaatgg ggcttgagac gataacgact actgcgttta cgtaacgtcc
    72301 cggcagaaaa gagctgttcg agcgcatttc tggctttttc cagcggccag ccaaagtggg
    72361 cggccacttc tcctgccgtc attccctgac atactgatgt taacagcgcc aacaactcgt
    72421 cagcagattc cgcgacgata actggcgcgt cgggttcctt tacgggttgc ttcggctggc
    72481 ggttaaagcc agtaaccagc cactgggtgt catcgtctgg ccgaccgatc tccttacggc
    72541 tgatgacacg cccggtacgc atcgcggcag cacaacctgc gtccagcgcc gcacgacacg
    72601 ccgccagatc gccttccacc accagcgtta agcgaccagg gtcaagcact tcgtgactga
    72661 gcagacgaac gttagctgct ttgagcatgg catccgctgc atctatcgcg gcgaccatgc
    72721 cgtccacttc cagcaatccc agtgcattga tcatcggcaa cctccgttac gcacgctgga
    72781 ttggattacg cgcgatttcc agcactgcat cggtaaaggc gttacaggct gctttacacg
    72841 cggcctggct accggttaaa aatgctgccg agtagttggt ttcagacggt ggcgggacgt
    72901 aggtcaccag ctgtacgtcg gcagatttca acgcggcatc aatgccgtag gtcgcttcca
    72961 gcggcggtgc caccagatac gccatcggat cgccaagcgt gatcccggcg gttgatgaaa
    73021 gataagaacc ggtacgcgaa accacatgcg ccaggaaggc ggtgttttct gcgtcattgg
    73081 cccactggaa agctgcgcca ttttcaatat gtgcaaccat cgcatccaga ccggcacgca
    73141 cttccgccgg gtttggccca ccaagcataa tcagcacttc accggcagtc ggtgacgggc
    73201 cgtgtgccgc tcccgcatac agcgagcgac catataccac ttcgaccatc gcttgtttgg
    73261 tcgcttcgtc agccgcaatg taggttacgt cgtcagaatc ggcagaaatc agcccgaggc
    73321 tacgaatatg cggcggcaat tttagttcac gcgcaaattc agcgttaacg gaggcaatca
    73381 cccgcatggc ggttaccgac ggtcgaatca aatctaaagc tggcatgatg cctccttatc
    73441 gggtcatgtt gatgccggat gctttctgct ccagcatacg tttcgccaaa tccacaatga
    73501 cggcacagca gcacgaaaga gaagcaaaag tcagctacct ggtacactag gcagcgggtc
    73561 agcaaagggg ggcaaatgcc ccttttttca tgtcagataa tcagatcatt acctcaataa
    73621 actaataatc acatcgcggt tacaaccagt ttgatagtga tagctcaaac ccagacgtgg
    73681 cgctggctgc aagcaaaata agccgcaaaa atgcgacccc atgtttacta gccgcaaaaa
    73741 tgcggtccta tttgccagaa ccttttatta ttcaattggt tagtgcaaga aaaagcgtga
    73801 ttactgttca agaagtatcc agaaaggtca tgattttgac gctgaaaaaa aataggtggg
    73861 catttttgcc cactcactag ccgcaaattt gcccactcac tagccgcatt tttgcggccc
    73921 aaagtaataa atacagagaa ataataaata cagatcagcg ccaagatttt tcgtcttttc
    73981 ggcggaatca tgagactagt gcttacgtcc tgataacctc cagagcagac cttaacttct
    74041 gtgcagcagg actacgctca ttgccgctcg gctaggtcaa atcctcacga taaagctgtt
    74101 cggattgtga ccataatgtt aaaggactct ttaaatcatt ccttgacgaa ttaactagct
    74161 ggcttaagcg gaatagcatg caggataaaa acaaaaccag cttagcagtg gggagagctt
    74221 cgcttcgctc gtctcctggg gccaatgctc aatgaaacga cgtaaggcac ggagcgctca
    74281 ccataggtag acacataaac atctgagagc gcaccttcta tcgcgctacg cgctcaataa
    74341 aaaccaacga caccgccaca ctgcaagacc aatggttttt atcgcgtata cagaccagac
    74401 aggaaacagc agcaaagcag cgtatagcca ggtgcagaca gcacactcac acgacgcaca
    74461 gcaggcttaa gtgatcactc aggaaacttt atccccgccg ccgatcaccg cgaactatca
    74521 tgaccgcgcc aaattcgaaa ggaatggccc atagaacgcg atgaacacac ataagcgcta
    74581 cacttactta acgtcgccac agagcgattg agagcgctta aaatcgatgc gatttcatta
    74641 aatttttctc actcgcctga atggtggcga tcaggactga tcgcaacatt gccccgcctc
    74701 gatattgagc gcataataga tacatacatt aagcggaaaa gagataagtt tattgtgaaa
    74761 atcagggggt tacatatcat gcgcatatca gaacaacaac ggcagatcct cgactatctg
    74821 gcaaaaaacg aatcaatcac cgcacgacaa gccgcagatc tcgtttatgg tggcaatgtt
    74881 acccgcgtcc aggttgacgc cgcccgccgc tcactcatga cgatggttag taatgggcta
    74941 atcaggaaac aaggccgcgc tttcgtagct gaaagacata tcaatcacgc ttatagccag
    75001 gacgttgaag aattgcgccg ccagatcctt tgggtattca gcaaacaggg tcaggagtat
    75061 ttcctttctg aacacccaaa tttcgccgcc aaagagttaa cagtaagcgg attgtcgtgc
    75121 gcactggatt atcttgtcgg tgaaggttgc gttacttaca agcagctatg gcacgccctg
    75181 aatacgcttg tagaagatgg tttactcatt gccacgcgtg agaacatcga atactggata
    75241 atgccgcgtt caggtcgccg caatcatgca cggtatattt caggcaacag atacagaacg
    75301 ccggacttta atccgacaca ggaagatcgg gaccgtgccg aaatgatgtg cagggaacag
    75361 gagaaaagaa ttatggataa gttattttcg atgcagtgaa cacaagcggg cgcaatgccc
    75421 gtttttattg ccttattcca cgcctgacga atcaatttga tcacacctga gaaaatgcat
    75481 agccagaagt taaggccgtt atattgcgat tgggagcgtt ttaaacggta tcaatttaat
    75541 taattctgat gtggtgattt gcaggcaaaa aaaacccgcc ggagaagcgg gaaatagaga
    75601 catatcgttg tagttattaa cagatcactt tgaggtcgag ggcggctaac cctcgctaat
    75661 attatcccgc tattaccttg cgggtgcaaa taatctttta ttgctttgat gatttaataa
    75721 atagcacttt ttgctaacaa aaactgctgt caatatatgg ccaaaaatgt caacagaaaa
    75781 aataaggttt ataatattaa gtatcacatg ggtaatgggt tcagggaatc ggaatccacc
    75841 gcctcacgga aattcaaaat ttgcttcttc tttcctccgt gggcggcttc tttttcgtat
    75901 cgaggtattt atgaaagcaa taacattaga acagttcgca gcaatggcga atgtatcgac
    75961 cagaacgctg aggcgctggc taaaaaaacc atcaaccgga gtccctttgc catttacccc
    76021 acgcggtact cgccaattat tctgggagca cgaatgcaaa gaatggttgt acagaacaga
    76081 acgataataa tcacgcatca cattttgagg taaacatcat gaccaatatc gaaagattag
    76141 aacgctgtgc cgatattatg cggcgcagat ggatttatga ccctgaagcg ggtttattgt
    76201 ttagccgtga aacaaaagac gttatcaaag gcagcatgac caataaaggg catttggttg
    76261 taacggtaca tgaaggtaaa ttcaaagtga cactcaccta tcagaaagcc tgctatgtat
    76321 acacatacgg cgcttatgac gagacattat atgaagtgca ccacgttaat ttgaacaaac
    76381 aggataaccg catcaagaat attcgcctga tgccgaaaga aaagcaccgc cggatacata
    76441 aaaacgtccg caaacttatt cggctgggcc acacacaatg gcttcaaccg caattaattg
    76501 aacaatcact ataaacacag gagtaaatac catgcattca ttagacagtt attttcagcg
    76561 cacgacagcc cctaaatcag ccgcccagga acgccgtgag gaattccatg agaaagtgat
    76621 gcgttctgct gattatattg cggacaagtt cgtggaaacg gttcgtccac ttgttgatga
    76681 agtagctgat aaacttcagt ctgaaatgcc ggaagatatg gaaggcactg caaagcggcg
    76741 actcatttgc gaattaagtc gtcgttttgg cgtgtcaata agcgctttta aataatcgaa
    76801 aaaagggggg aaagataatg gacgcatttg aggtactggc aaataaaggc gctgaactgg
    76861 tagcacagcg tgataaaacc gccaacgaag gggaacgcgc actgctaaat aaacagatta
    76921 aagcgatccg tatggcccag tttaaattaa tcagtaatga agttatcgaa acaattaaac
    76981 cgcagatccc acaaatcgtt gctgacgcta ttaaagccgc cggactggag aaacgcattg
    77041 ccgggctaaa aacgaacgca gagaaaatgg agatgcacaa acagagcggt gctccgaatc
    77101 ccgatgagta cttctcaccg gatgttgagt ttatggcgca ggttgaggaa cgtctgggta
    77161 gctgtctcac cgaggaacag cgtcgctatt ttgatggagt ggatagttcg gcaggtattg
    77221 acctgaactc gtatttcggt cgtgaaattg agcattttga tgccgccccg tctatgccgg
    77281 agccagaacc agaaatggcc accgatcctg aagctgagca acggaaacgc gtattcaatg
    77341 cgatagggat tcggtactga tcggataaaa gaaagccggg aattaccccg gctgacttaa
    77401 caatgtgacc aatgaacaaa tcggacaaga caaacaggat aagcagtctc gaaacaatgt
    77461 tcgggaacta tattagcaca agtccggaga aaatcatcat gttggcacta cgcatcatgc
    77521 agggcatcgc taagaccctc gcagaacatg tcttagacct gaaacattcg ccactgagca
    77581 aacaggccat gaagcgccaa acgcttcgtc tgtgggctga atattcgctt ggaactatca
    77641 ataaaatcat cgacatgaaa tcggggccga gcaaccagag cgcggaggaa atggagttca
    77701 tcaggcgttt aattctcatt cgcagagaca ttcatagcca gttacacagc gtgggaatcg
    77761 atattaatga tgggacgggc gattaatttt cggacaagta ctatgcgcgc gcgaggagct
    77821 gaacatgaag caagatattg cagacaggct ggagatactc gaaggccagc gggcagaagc
    77881 gaaacaacta cgtaagcagg ccagaagagc gcacaggaac aacgaagcag agttactaac
    77941 caaatacatc agtttcacca attactgcat ctacgagtgt tacaaggaag atgcagagga
    78001 ctggctggat tcattgccag aacagtactg agcagaataa caccacgtca tgttgttctg
    78061 aggtgagcat atcacctctg ttcaggtggc caaatttagt gtgccacttc agcatcaccg
    78121 ttgaaatcac aataacgatt tttttgtaaa tatgctctca catatgtagg aatagaccag
    78181 gttggcattg aaacacatct accatgacca taataaagta attttttgat atgtttatgg
    78241 ttttatatta attaaaatat atctaaaatt gatagtttta aaattgcaaa atccagattg
    78301 tgtttgtaat gtttaatctg attgtattga gtggcgttta ttatgaaaaa taaatcaggt
    78361 aattcttgtt atagagaagc agtcgatatt catagctcac ttgagctttc atttatattg
    78421 cataaatatc atttgcgtgt agatgagaag aaatctcgaa atgtattcag cgccatacgt
    78481 gatgagctca tccttgatgg ggattcacga caaaacttag ctactttctg tcaaacatgg
    78541 cttgatgatg aaatccgtga gttgcttaat ttagctattg ataaaaacat gatttataaa
    78601 gatgaatatc cacaaatggc ggaacctgaa ggtcgctgtg tgcgtatgct ttccgattcg
    78661 tggagtttcc ccgattcgcg acacacctta ggttgctcaa ccattggttc atctgaagct
    78721 gctatgctcg gccgcttggc gttaaaatgg cagtggtgta aaaaacgcga agtacaaggt
    78781 aaatcaaccg aacctgattt gcgatcctgt gcaaatatgc tggcataaat tctgccgtta
    78841 ctgtgatgtg aaattgtgtg aaattccact tgatgatcat cacctgataa tgaatgctaa
    78901 agaggttttg aagcatgtcg atgaaaacac tattggtgtc gttacgactt taggtattac
    78961 ttatttttgt aaagcagatt gcgttagtgt ttttaagata agtgttaaag ttaatattag
    79021 tttttatatt tacatcgtaa ttatatgttt ctattttaaa aatgttcgag agtataaaaa
    79081 tgtctaatgc taatatcgca cccaaaagta agaaaatgac agtggggaca ctcgctatca
    79141 tgaatataac tgctgtagta agtctacgat ttataccttc tgaagcagaa tacggcttag
    79201 gtgccatttt ttactatacc tttgctgcaa ttatgttttt agtacctgtt gcctttgttg
    79261 cggctgagct agctacaaca tatccacaaa aagggggggc atttcgatgg ataggagaag
    79321 catttggggc tcgctggggt tttgtaggaa tgcttatgac ctggctacaa gtcatcccct
    79381 actttccaac agtactgaca tttggtgcag tctcggttgc atttattgat gttgatatgg
    79441 gggtggcaga atcaattgcc agtaataaat ggtatatttt atttttcgta ctatttgttt
    79501 actggggagc tatttgtgtt gcattacgtg gcgttggtat tttctcgcag gtatctaagt
    79561 ggtgcggtat tatagggaca attattccag ctattatcct tgttattctt ggtttctcct
    79621 atctgtggtt cagcggtaaa ccagcggtga tcccattagg ttggggtgat cttttcccta
    79681 atatagctaa cttccagaat attgttcttg ccgccagcat atttcttgct tatgcaggga
    79741 tggaaatgaa tgcggtccat gttaacgatt tagataatcc aactaaaaaa tacccaattg
    79801 caattaccat cgcttctata ggtactattg caatattcct gctcagtact ttaggggtcg
    79861 cctttattat tcctacagat aaaattagtc taactcaaag tttgttggtt gcctacgata
    79921 tgctgttcga atgggctgga gtgccatggc ttggctctgt gatggcattt atgctagcaa
    79981 ttggcgtact gggtggggtg gttacttgga ttgcagggcc taatacaggt gtattagcga
    80041 tcgcgaaagc tggttattta cctaagtttt tccaaaaaac caatcgtcat ggaatggggc
    80101 accatctaat gtttgtacaa ggtataattg tctcagttct ttctgttact tttgtgataa
    80161 tgccttcagt tcaagcagct tttcagattt tatcacagtt aacagttatg ctctatttag
    80221 ttatgtatat gctcatgttc gctagtgcta tctatctacg acattctcaa cctgatacaa
    80281 aacgcccata tcgagcccct gctttatttt tctgggcaac acttggattc ttagggtctt
    80341 ttctggcttt tgtattaagt tttattccgc ccgagcaaat cccaactggt agtccaacca
    80401 gctatgttct ttatttgtta gtattagttg ccctattttt attaattcca atattaattt
    80461 acgccttccg aaaatctgac tggaaagatc caaactctga tttcgaacca ttttcttggg
    80521 aaaaaagaac tagtaattaa attttccata agctacaaaa gatgaggtta aatatgttta
    80581 gtgctgaatc tccaattggt gtaatagact caggtgtagg tggattgaca tcggtaaagg
    80641 aaataattaa tttgcttcct ggtgaagata ttatctattg tggagataat atgaatgcac
    80701 cttatggcaa ccgttctgcc gatgatatca tcagtttgac taaaaaaatg ctgacttttt
    80761 tacagtcaag gaatgtcaaa ctagttgctg tcgcatgtaa tactatatcc tcaactttgg
    80821 aatccgaaga atacagcgga tatgctaaga gctttccgtt tcctatctta tccatcattg
    80881 aacctgcagt agaagatgtg attcgccagc aatataaaaa tgttggtatt atcgctactg
    80941 agtttactat taaaacgggg tgtcacaaag agctaataaa aaaattgaat tcaacaataa
    81001 atgtatttgg agaaccaagc aaaaatcttg caatgttaat tgaggaaggt aatcttaatg
    81061 ctcctgcaat cttgaatgac atcaaaaaac atgttaacca tttaatttcc ctccatccag
    81121 taaatgaaat tattctcggc tgtactcact atccaattgt tcagaatttt tttgaagcag
    81181 ttgcaccgga tataaaattt attaatcctg cacatgatca ggctatctct attaaaaatc
    81241 acttgggaca gctaaattta ttaaactcct caaatatagg aactttgcaa ataaatacta
    81301 gtggaagtat ggagatctac aaaacggtac taggggaatt gagcattacg aaacctcata
    81361 cattcagtat caggcagttt taaatttatc cggtgcaccg taaaatcccg tccttctgaa
    81421 ctgcacccca aaagttggac accaactgag taaggtgcat tttatggaca aacccagtta
    81481 catactgggt agtgcatcca attagtagaa catgtgtttt tcgataaacg ctccgatcac
    81541 tttttcgtgg atctcaactg agcgtgagaa gcagattgtt ttacgaccca accgcttaat
    81601 gcgggtgcgt agcgtcagat tattacgctc aatgcgttgg gtgaatattt tgccggtcag
    81661 atgcttattc ttcggcacct cccggccata gctgccccag tcatcgctgg tcagcatgcc
    81721 gatgttgaag ggtgtaagca gtgccagtag ctcccggcac gtttgatcgg ttcggggacc
    81781 aaaagtgtag gccagtacac cgcctgtttt ggtgttgtac gcgtacccga gccaatgttg
    81841 ccgggcttta ctgccaacgt agctccattg ctcatcaagc tcgcagataa acgccacatc
    81901 agcatgggca acaggcgaag aagttattcg ctttggcgcg agttttttaa agtccggatg
    81961 acggtgttac taccaatttt cagtgtcctg gcggtatcgc gaaccccggc accattaaag
    82021 gccatttcag ttatcagctc tttcataccc ggcttacgtg cttgataagt ataagtgaac
    82081 tgaaacacac ggtggcagtc acggcagcga agtctgtcac ggcctttagg gttctgacca
    82141 tggcggtaaa cctgagctga ctgacaacgg ggacaatgaa tattaacgct ggtcatgaga
    82201 taacctcaaa agcccgtatt atacatcaga ttcaactaat tagaggcatc acccatccag
    82261 cattataaaa catactaaca ataactaaat tataataatc acaacttaca atcaaataat
    82321 aaatgcataa gattaacaat gattttttcg ggttatattt atctaaaaaa gcagaatatt
    82381 atgtaactta ttattttcta agcgaaaatt tattgtgcgg tcagactaac aaacttgcgt
    82441 accacccgaa gaacatgcga agatttaagt attctttctt ttttggtttt tatttttcat
    82501 tttctctttt cttctctttc cggttctgtt ctggttttgg ttttttcctt tttattttta
    82561 ttctttcttc agggttatgc taaaacacca aaacattttt caccacaaaa gcatccaatc
    82621 atcttgccag accaatcacc ttaaccggat acactgccgg atataatccc actaacaccc
    82681 agacaccaag caccatacct gataactacc gtattcagga aggaaatgcg agtgatttca
    82741 ccgtcaccgg cacaaccgat ccgccaaaaa gaggcgtacc aatgtcgata tagtcccccg
    82801 cgcggacaat cacttcgtca atgactgcca acgggagttg ttgtagctgt gggcgcaaca
    82861 acatgcccag agctttacca aagtcctgcc cggccaccac cagcagggga tgcggattcg
    82921 gaaaacgcgc gacgaaatcg accagcgcgt tgatgaccgt cagaaccgcg gcgtaacgca
    82981 caggcagcga ggcgggaagc gccagcacgt acacgtcagt tttgggatca agatccagct
    83041 gaagcagcgc ctgttgccag gcactcacca gatccgtttc atcaatcggg atcgccaccg
    83101 gcaaattgcg cagcggcagt tgtacgccct ccagccagat tgtgctgcca gagagcgaaa
    83161 gggtatgtgc acccgcgcca atcaccgtgg agcgtacggt ttgcgccgga aactgcacat
    83221 tcatctcacg caggcgcgga tggtcatgca gcgccgttgc cagaagtggg ccaatatcgg
    83281 caaaacagaa cgggttggcg ggctggtggc gataacattc gcccacgcct ccagaaagcg
    83341 taatgatttc gggcgtaaca cctgcgggca gcaaaccggt ttgcatcaat gcctgcgcga
    83401 gcggcgaaag cgttccgtca atcacttcga caatcagcgc cgccatccgc cgggtaacct
    83461 gcaccagctg cgcgccggtc agcgaacggg cgtcagtgcc tgcaccgaag cactcatcca
    83521 caatcatctg ccccggttta tgagcgtaaa ccacgcgccc ctggctgtcg gtttccagca
    83581 ggcgaccacc gacgttgaga caggcagtgc cgctgatttt tccggcatcg aacagggcgt
    83641 agttcgcggt gccaccgccg atatcgatat tcagtacccg acacagccgt tgttcagaaa
    83701 gggtttgcgc cccggctccg tgaccggcga tcacggattc gaggtgcggc ccggcgctgg
    83761 cgacgacaaa atcgcccagc gactgttttt ccttcatcac tttttccatc agctgtgcga
    83821 ccaggctttc ggtaaactgg ccttccggca gctgggcaat gatggtttcg cggatgcgct
    83881 ggctttcggt tttgtcggct ggcacgctgg cgggcacgga cgccgttacc gggatcgatt
    83941 catcacattc ggtgatggta aaacccagta gatccgccac ttcacgggct tccggggtaa
    84001 taatgctggc gcgcagaacc acggacattg cctgttcgcc gcgcgcgtgt gcttcacgaa
    84061 tatcattcgc tgtgatgagt tttttcacct gcttcctcct gtttggtgag agatgccaga
    84121 taatccacca gttgctgcac gctttgcggg tcgtggctgt ttagctcgaa aatcggctct
    84181 tcgaaacccg tctcaagcaa taactttcgc gttgcggcga cgtcagcatc tggcatgtcc
    84241 gttttactga tgacggcgat ttgccgtttg ctgacgccaa tatccagcaa cccggcaggc
    84301 aaccgacttt ccgggtcatt tgcgccatgg acatagatca gcatgtctac atcctgtagc
    84361 gtggtaatta aggcgtgata ccagcgggga tggctaaaat attcacccgg cgtatcaata
    84421 tcgcccttat cattaaattc cacggccagt gtttttctgg cgagggtata attcccctgc
    84481 aacgcattaa ataacgttgt ttttcctgcc ccgacgctac ccacaaacgc aattcgtttc
    84541 atgaatgc
//
LOCUS       DAJVRP010000002        78784 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVRP010000002 DAJVRP010000000
VERSION     DAJVRP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.002"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..228
                     /locus_tag="OQN12_000087"
     CDS             <1..228
                     /locus_tag="OQN12_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCS1652569.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            648..1263
                     /locus_tag="OQN12_000088"
                     /pseudo
     CDS             648..1263
                     /locus_tag="OQN12_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1366..1689
                     /locus_tag="OQN12_000089"
     CDS             1366..1689
                     /locus_tag="OQN12_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCS1652570.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OQN12_000090"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OQN12_000090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1652571.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OQN12_000091"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OQN12_000091"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1652572.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OQN12_000092"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OQN12_000092"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1652573.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OQN12_000093"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OQN12_000093"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1652574.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OQN12_000094"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OQN12_000094"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1652575.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OQN12_000095"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OQN12_000095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1652576.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OQN12_000096"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OQN12_000096"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1652577.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OQN12_000097"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OQN12_000097"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1652578.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OQN12_000098"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OQN12_000098"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1652579.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OQN12_000099"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OQN12_000099"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1652580.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OQN12_000100"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OQN12_000100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1652581.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OQN12_000101"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OQN12_000101"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1652582.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OQN12_000102"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OQN12_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1652583.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OQN12_000103"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OQN12_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1652584.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19269..20486
                     /gene="alaA"
                     /locus_tag="OQN12_000104"
     CDS             19269..20486
                     /gene="alaA"
                     /locus_tag="OQN12_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1652585.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20570..21169
                     /gene="yfbR"
                     /locus_tag="OQN12_000105"
     CDS             20570..21169
                     /gene="yfbR"
                     /locus_tag="OQN12_000105"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1652586.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21228..23060)
                     /locus_tag="OQN12_000106"
     CDS             complement(21228..23060)
                     /locus_tag="OQN12_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1652587.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OQN12_000107"
     CDS             complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="OQN12_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCS1652588.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23808..24302)
                     /locus_tag="OQN12_000108"
     CDS             complement(23808..24302)
                     /locus_tag="OQN12_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1652589.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24385..24840)
                     /locus_tag="OQN12_000109"
     CDS             complement(24385..24840)
                     /locus_tag="OQN12_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1652590.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25178..26380
                     /gene="ackA"
                     /locus_tag="OQN12_000110"
     CDS             25178..26380
                     /gene="ackA"
                     /locus_tag="OQN12_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1652591.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26455..28599
                     /gene="pta"
                     /locus_tag="OQN12_000111"
     CDS             26455..28599
                     /gene="pta"
                     /locus_tag="OQN12_000111"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1652592.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28789..30309
                     /gene="yfcC"
                     /locus_tag="OQN12_000112"
     CDS             28789..30309
                     /gene="yfcC"
                     /locus_tag="OQN12_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1652593.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OQN12_000113"
     CDS             complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="OQN12_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1652594.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OQN12_000114"
     CDS             complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="OQN12_000114"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1652595.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OQN12_000115"
     CDS             complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="OQN12_000115"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1652596.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32329..32976
                     /gene="yfcG"
                     /locus_tag="OQN12_000116"
     CDS             32329..32976
                     /gene="yfcG"
                     /locus_tag="OQN12_000116"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1652597.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33033..33395
                     /gene="folX"
                     /locus_tag="OQN12_000117"
     CDS             33033..33395
                     /gene="folX"
                     /locus_tag="OQN12_000117"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1652598.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33416..34309
                     /locus_tag="OQN12_000118"
     CDS             33416..34309
                     /locus_tag="OQN12_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1652599.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OQN12_000119"
     CDS             complement(34357..35247)
                     /gene="rpnB"
                     /locus_tag="OQN12_000119"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1652600.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OQN12_000120"
     CDS             complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="OQN12_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS1652601.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36225..36941)
                     /locus_tag="OQN12_000121"
     CDS             complement(36225..36941)
                     /locus_tag="OQN12_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1652602.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OQN12_000122"
     CDS             complement(36938..37624)
                     /gene="hisQ"
                     /locus_tag="OQN12_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1652603.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OQN12_000123"
     CDS             complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="OQN12_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1652604.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OQN12_000124"
     CDS             complement(38717..39499)
                     /gene="argT"
                     /locus_tag="OQN12_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS1652605.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OQN12_000125"
     CDS             complement(39765..40334)
                     /gene="ubiX"
                     /locus_tag="OQN12_000125"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1652606.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OQN12_000126"
     CDS             complement(40429..41946)
                     /gene="purF"
                     /locus_tag="OQN12_000126"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1652607.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OQN12_000127"
     CDS             complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="OQN12_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1652608.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OQN12_000128"
     CDS             complement(42730..43392)
                     /gene="dedD"
                     /locus_tag="OQN12_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1652609.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OQN12_000129"
     CDS             complement(43382..44650)
                     /gene="folC"
                     /locus_tag="OQN12_000129"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1652610.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OQN12_000130"
     CDS             complement(44720..45634)
                     /gene="accD"
                     /locus_tag="OQN12_000130"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1652611.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45790..46449)
                     /locus_tag="OQN12_000131"
     CDS             complement(45790..46449)
                     /locus_tag="OQN12_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1652612.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OQN12_000132"
     CDS             complement(46532..47344)
                     /gene="truA"
                     /locus_tag="OQN12_000132"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1652613.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47344..48357)
                     /locus_tag="OQN12_000133"
     CDS             complement(47344..48357)
                     /locus_tag="OQN12_000133"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1652614.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OQN12_000134"
     CDS             complement(48423..49559)
                     /gene="pdxB"
                     /locus_tag="OQN12_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1652615.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49658..50653
                     /gene="flk"
                     /locus_tag="OQN12_000135"
     CDS             49658..50653
                     /gene="flk"
                     /locus_tag="OQN12_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1652616.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50650..51828)
                     /locus_tag="OQN12_000136"
     CDS             complement(50650..51828)
                     /locus_tag="OQN12_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1652617.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OQN12_000137"
     CDS             complement(52083..53324)
                     /gene="fabB"
                     /locus_tag="OQN12_000137"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1652618.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53483..55489
                     /gene="mnmC"
                     /locus_tag="OQN12_000138"
     CDS             53483..55489
                     /gene="mnmC"
                     /locus_tag="OQN12_000138"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1652619.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55610..55888)
                     /locus_tag="OQN12_000139"
     CDS             complement(55610..55888)
                     /locus_tag="OQN12_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1652620.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OQN12_000140"
     CDS             complement(55922..56470)
                     /gene="epmC"
                     /locus_tag="OQN12_000140"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1652621.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56470..57279)
                     /locus_tag="OQN12_000141"
     CDS             complement(56470..57279)
                     /locus_tag="OQN12_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1652622.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OQN12_000142"
     CDS             complement(57279..58103)
                     /gene="mepA"
                     /locus_tag="OQN12_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1652623.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OQN12_000143"
     CDS             complement(58107..59192)
                     /gene="aroC"
                     /locus_tag="OQN12_000143"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1652624.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OQN12_000144"
     CDS             complement(59227..60159)
                     /gene="prmB"
                     /locus_tag="OQN12_000144"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1652625.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60325..60876
                     /gene="smrB"
                     /locus_tag="OQN12_000145"
     CDS             60325..60876
                     /gene="smrB"
                     /locus_tag="OQN12_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1652626.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60980..61870)
                     /locus_tag="OQN12_000146"
     CDS             complement(60980..61870)
                     /locus_tag="OQN12_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1652627.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61857..62381)
                     /locus_tag="OQN12_000147"
     CDS             complement(61857..62381)
                     /locus_tag="OQN12_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1652628.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62378..62848)
                     /locus_tag="OQN12_000148"
     CDS             complement(62378..62848)
                     /locus_tag="OQN12_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1652629.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62845..63393)
                     /locus_tag="OQN12_000149"
     CDS             complement(62845..63393)
                     /locus_tag="OQN12_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1652630.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63398..64120)
                     /locus_tag="OQN12_000150"
                     /pseudo
     CDS             complement(63398..64120)
                     /locus_tag="OQN12_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64140..64862)
                     /locus_tag="OQN12_000151"
     CDS             complement(64140..64862)
                     /locus_tag="OQN12_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1652631.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64922..66154)
                     /locus_tag="OQN12_000152"
     CDS             complement(64922..66154)
                     /locus_tag="OQN12_000152"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1652632.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(66203..66781)
                     /locus_tag="OQN12_000153"
                     /pseudo
     CDS             complement(66203..66781)
                     /locus_tag="OQN12_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66863..67426)
                     /locus_tag="OQN12_000154"
     CDS             complement(66863..67426)
                     /locus_tag="OQN12_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1652633.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68110..68595)
                     /gene="sixA"
                     /locus_tag="OQN12_000155"
     CDS             complement(68110..68595)
                     /gene="sixA"
                     /locus_tag="OQN12_000155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1652634.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68798..70942)
                     /gene="fadJ"
                     /locus_tag="OQN12_000156"
                     /pseudo
     CDS             complement(68798..70942)
                     /gene="fadJ"
                     /locus_tag="OQN12_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70942..72252)
                     /gene="fadI"
                     /locus_tag="OQN12_000157"
     CDS             complement(70942..72252)
                     /gene="fadI"
                     /locus_tag="OQN12_000157"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1652635.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72432..72716)
                     /locus_tag="OQN12_000158"
     CDS             complement(72432..72716)
                     /locus_tag="OQN12_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1652636.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73088..74428
                     /gene="fadL"
                     /locus_tag="OQN12_000159"
     CDS             73088..74428
                     /gene="fadL"
                     /locus_tag="OQN12_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1652637.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75179..75853
                     /locus_tag="OQN12_000160"
     CDS             75179..75853
                     /locus_tag="OQN12_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652638.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(76035..76790)
                     /gene="mlaA"
                     /locus_tag="OQN12_000161"
     CDS             complement(76035..76790)
                     /gene="mlaA"
                     /locus_tag="OQN12_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS1652639.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            77084..78016
                     /locus_tag="OQN12_000162"
     CDS             77084..78016
                     /locus_tag="OQN12_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS1652640.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            78090..78164
                     /locus_tag="OQN12_000163"
     tRNA            78090..78164
                     /locus_tag="OQN12_000163"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78123..78125,aa:Arg,seq:cct)
     gene            78326..>78784
                     /gene="intS"
                     /locus_tag="OQN12_000164"
     CDS             78326..>78784
                     /gene="intS"
                     /locus_tag="OQN12_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCS1652641.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT        19967 a        21039 c        19326 g        18452 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctataaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa aaaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac
    19141 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
    19201 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
    19261 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
    19321 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
    19381 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
    19441 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
    19501 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
    19561 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
    19621 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
    19681 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
    19741 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa
    19801 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
    19861 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
    19921 ctgagcatca ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac
    19981 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
    20041 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
    20101 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
    20161 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
    20221 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
    20281 tcgacgccaa acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc
    20341 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
    20401 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
    20461 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
    20521 tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
    20581 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
    20641 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
    20701 ggcagctatc aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact
    20761 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
    20821 cttcaatgcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact
    20881 ggtcgatatg gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc
    20941 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
    21001 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
    21061 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
    21121 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
    21181 ctgcgtcgca tcagtcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
    21241 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
    21301 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa
    21361 gacaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa
    21421 ggcgacactc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
    21481 gcggtattag agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc
    21541 atatgtggcc cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct
    21601 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
    21661 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
    21721 caggcgatga tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt
    21781 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
    21841 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
    21901 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
    21961 ccttccagcg ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga
    22021 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
    22081 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
    22141 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
    22201 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
    22261 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
    22321 tccagccgtt gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
    22381 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
    22441 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
    22501 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
    22561 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
    22621 ctaatcagcc cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc
    22681 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac
    22741 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
    22801 gccactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
    22861 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
    22921 tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
    22981 tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc
    23041 caaatcaatt caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc
    23101 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
    23161 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
    23221 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg
    23281 ccaggccaga aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa
    23341 gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc
    23401 gctcagcggt tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg
    23461 ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat
    23521 tgagtaaggc gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt
    23581 gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc
    23641 gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc
    23701 catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg
    23761 ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata
    23821 atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta
    23881 agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg
    23941 tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt
    24001 gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg
    24061 tcctgcaaat tagaccagga aacatacaac gcatgataca tctccataat gtcgatgata
    24121 gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat
    24181 ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg
    24241 agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc
    24301 attgttgact cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct
    24361 tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt
    24421 gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc
    24481 caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc
    24541 agggggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact
    24601 taaggcgaac agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca
    24661 gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat
    24721 aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta
    24781 atgctgtccg cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat
    24841 gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg
    24901 gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga
    24961 aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg
    25021 aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc
    25081 agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg
    25141 acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt
    25201 tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga
    25261 gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat
    25321 ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa
    25381 ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat
    25441 cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc
    25501 tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct
    25561 gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt
    25621 atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta
    25681 caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta
    25741 tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac
    25801 ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac
    25861 ctctatgggt ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga
    25921 tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa
    25981 actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta
    26041 tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca
    26101 ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt
    26161 tgcattcact ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa
    26221 actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa
    26281 atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga
    26341 actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc
    26401 tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc
    26461 cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc
    26521 gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag
    26581 ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc
    26641 accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat
    26701 cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct
    26761 gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg
    26821 aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact
    26881 gacaccccgg aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc
    26941 aaaaacacca acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag
    27001 ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac
    27061 aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg
    27121 tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc
    27181 atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc
    27241 agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac
    27301 cgtcctgacg tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc
    27361 ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct
    27421 ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc
    27481 ctgcagagct tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag
    27541 gaatacgttg ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag
    27601 cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa
    27661 gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct
    27721 atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac
    27781 cgtgttgcag cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa
    27841 gtggttcgcg aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc
    27901 gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa
    27961 caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt
    28021 ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc
    28081 atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc
    28141 gctgaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc
    28201 gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta
    28261 gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac
    28321 ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac
    28381 tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc
    28441 acctacaaag cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt
    28501 atgcgcaaac cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc
    28561 atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt
    28621 tgcgctgcgg atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt
    28681 tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct
    28741 cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc
    28801 actgaatcca aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt
    28861 gttgctattt taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag
    28921 gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca
    28981 tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg
    29041 acgggcgatg aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg
    29101 aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc
    29161 attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc
    29221 gggaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta
    29281 tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg
    29341 ctgggctatg acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt
    29401 gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg
    29461 gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg
    29521 atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat
    29581 gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt
    29641 ggtgactggc tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg
    29701 atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg
    29761 attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca
    29821 tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa
    29881 gggattttgc tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc
    29941 ctcaacagca ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg
    30001 ttgctcttcc aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta
    30061 actatgccgt tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg
    30121 ctggcgttcc agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta
    30181 atggcaacgc tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc
    30241 ctgcttggac tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc
    30301 taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc
    30361 tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct
    30421 ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca aactccactg
    30481 acttcatctt cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag
    30541 acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca
    30601 atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga
    30661 actacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga
    30721 cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc
    30781 agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg
    30841 acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct
    30901 ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc
    30961 tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg
    31021 ccataacccg ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa
    31081 atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc
    31141 tggtttaaag caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt
    31201 tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga
    31261 tccacttcgc tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc
    31321 cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc
    31381 aacacgtcgc caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca
    31441 cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac
    31501 tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg
    31561 cttcgccgaa agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata
    31621 atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc
    31681 cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa
    31741 atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc
    31801 ttttttcgcc cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc
    31861 gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata
    31921 aatacgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga
    31981 ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc
    32041 ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga
    32101 caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc
    32161 atctgaccaa agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg
    32221 ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg
    32281 cgctttctat acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc
    32341 tatttcgccc cgacacccaa tggtcacaaa attacgctgt ttctcgaaga agcagagctt
    32401 gattatcgct tgattaaggt agacctgggg aaaggcggtc agtttcgtcc ggaatttttg
    32461 cgcatttcgc ccaacaacaa aattccggca attgttgatc attctcctgc cgatggcggc
    32521 gaaccgttaa gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga
    32581 ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag
    32641 gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agccccccaa
    32701 accattcctt acgctattga acgttatcag gttgaaactc agcgtcttta ccatgtacta
    32761 aacaagcggc tggaaaactc gccctggctg ggaggcgaga actacagcat tgcggatatt
    32821 gcctgctggc cgtgggttaa tgcctggact cgcctgcgaa ttgacctcgc aatgtatccg
    32881 gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca ggcactgcta
    32941 aaagcacaac tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat
    33001 ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta
    33061 taaagaacct tcgtttgcgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc
    33121 aggatattgt tatcaatgtg acgatccact accccgccga taaagcgcgc actagcgaag
    33181 atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa cattattcag catgtagaga
    33241 ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac
    33301 atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg
    33361 attcggtatc catgacctta agctggcagc gttaatcgcc atatcgggag gctgcatgaa
    33421 catagtgatc accggaggga cgggattaat tggtcgccat ttgattccgc gtttgctgga
    33481 gctgggccat caaattacgg tagtgacgcg taacccgcag aaagccagtt ccgttctcgg
    33541 ccctcgggtg acactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc
    33601 ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcaca agcaaaaaga
    33661 gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga ttaatgctag
    33721 cgacacgccg ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg
    33781 tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc
    33841 ccgctgggaa gagattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg
    33901 aaccggcgtg gtactggcac cggacggcgg tattctcggt aaaatgctgc caccgtttcg
    33961 tcttggcctg ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga
    34021 tgatatggtc aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat
    34081 ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgcgctgca
    34141 tcgcccggct attttgcgcg tccccacaac cgccattcgg ctgttaatgg gcgaatcttc
    34201 agtactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc
    34261 gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttaca
    34321 gcaaacatcc gccagttaac tccctgtgtt acaggattag tggctttgcg cgataagatc
    34381 gtctggtgaa agtcgggtca ccatcataac taactctctg tctaaacctc tatccagcat
    34441 ctcctgagca atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa
    34501 tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc
    34561 aataaatgca cggaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt
    34621 tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc
    34681 caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg
    34741 ttgcataatc tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata
    34801 tattttgcgg gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata
    34861 aggacttctg caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc
    34921 cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa
    34981 ttcttccggc ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt
    35041 tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt
    35101 tggttctagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat
    35161 attaataaaa ttccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg
    35221 cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg
    35281 cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat
    35341 tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt
    35401 ccccttgaaa atactccggc agcgttagcc accggagagg aaattatttc agcgatccct
    35461 taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct
    35521 cttcttctat tttcccctga tggaggaaaa tgacgtgagt agaaacatgg cgagcaaagc
    35581 ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac
    35641 gtaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa
    35701 cttctggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa
    35761 gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gactttcgcc agatacttca
    35821 ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga
    35881 cgttttccaa caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc
    35941 gtgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc
    36001 gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcactcggt ttttcgagga
    36061 agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta
    36121 catctccggc attcgcttgc agtgagaccc ctttcagcac ttcgtgttcg ccatagcgtt
    36181 tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa
    36241 ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa
    36301 gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg
    36361 gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcgca
    36421 gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca
    36481 gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc tctggcggct
    36541 tcaatttccc catgcggaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg
    36601 caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta
    36661 aggaattcgg ttcccttaac aatctcaagc gtgtacatgc cggaatagaa caccagcaac
    36721 tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg
    36781 tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact
    36841 accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc
    36901 cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac
    36961 ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg gtgaaaacca
    37021 ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca
    37081 gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga
    37141 ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca
    37201 tgatccgccg aaacacttgc ccgcgagtaa aaccgaacgc cgtcgccgcc tctatatgcc
    37261 ctttcggcac tgccataaac gcgccacgga acgtttcggt aaaataagca ccgtagataa
    37321 aaccgagagt gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg
    37381 cctccgtcac catgtttagc gcaatctgca aaccatagaa aatcagcagc atcaacacca
    37441 agtccggcac gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat
    37501 tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc
    37561 tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccataca
    37621 acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc
    37681 cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat caaaatcgaa
    37741 gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt
    37801 cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa
    37861 cagtttttca tctttaacag acggaccacc gaatttgtaa tctttaccga cgggttgttt
    37921 gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt
    37981 caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc
    38041 ccagtgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc
    38101 tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca gacgagaatc
    38161 ggcagcgtac agtttgtcgg tgaaggcaat ttcttgctga cgtttttccg taatggaaag
    38221 cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg
    38281 gttttcgaca aacgtacatt gcgtattgat gcgcttgcac aactccttcg ccagctcgat
    38341 atcgaaacca accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc
    38401 ggtaccgatg cggatgtttt gcggaattgc agcaagtgcc gcagttgcgc tggagaaggc
    38461 cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt
    38521 tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca
    38581 aaatatcccg ccccgttaga cgtattattt ttcattaaaa atgcttaagt atgtatttca
    38641 atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc atcaggtaca
    38701 gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct
    38761 tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt
    38821 cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta
    38881 ctgatgagcc agcaaaagcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg
    38941 cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca
    39001 aatcctggtt ggcataggcc accacatcca cgcctttact acgccaggtc tcgttagcgt
    39061 aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg
    39121 ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt
    39181 cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatagcgt
    39241 cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acccaggtac
    39301 atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat
    39361 cacctttagc atctttcgac gagaacagcg cataggtggt atcggttccg atacgtaccg
    39421 tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaat aaagacagag
    39481 cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat
    39541 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
    39601 acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
    39661 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
    39721 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
    39781 cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc
    39841 tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac gggaggcata
    39901 atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt
    39961 gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg
    40021 gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt
    40081 aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc
    40141 gcatcgagcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg
    40201 gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg
    40261 cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca
    40321 atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg
    40381 gcgggagttg caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt
    40441 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg
    40501 taacgtatcg aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt
    40561 gaacaccgag cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag
    40621 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac
    40681 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
    40741 gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
    40801 catctcgata atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac
    40861 gtttttatcg cggaactcgg cgcggttggc attcagttta cggcgcacgg acttacgacg
    40921 cagctgctgg cccggcatga taaaggtgcg gccaacatag cggtttttaa cgaagccctg
    40981 gcggtacggt ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttctgg
    41041 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag
    41101 tttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg
    41161 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
    41221 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
    41281 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
    41341 acggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg
    41401 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa
    41461 gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa
    41521 gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat
    41581 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
    41641 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
    41701 gctggagctg ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
    41761 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
    41821 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
    41881 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
    41941 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa
    42001 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga attgcgggat
    42061 cagctgtgat ttgctccagt cttcactttt cgacaccccg gtaaaggagt cgagaaagaa
    42121 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
    42181 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
    42241 accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc
    42301 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
    42361 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgtgga tcaggctaac
    42421 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc
    42481 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
    42541 cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
    42601 cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta
    42661 tccgacctac ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc
    42721 aagactgtat taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt
    42781 caactcaccc agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg
    42841 ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg
    42901 cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac
    42961 ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg
    43021 cgcaggtggt gcttcaacct ttggtttagg cggctccacc ggtttcggct tcggtggggc
    43081 gaccggtgca ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag
    43141 cgacggtgct gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt
    43201 cggcaacgcc tgggtggcgg ctggcatcat atcaggctca tcacgatcgc ccgctttcgg
    43261 caccagcggg atagccgcga actcatcctg ataatgtttt ttctgcccgt ccagcagccc
    43321 tggaagtaca atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa
    43381 cttacttgcc accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga
    43441 aagaaccaca caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg
    43501 cctgcgcaac gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg
    43561 tggcaccgcg cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc
    43621 aggccagagt tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc
    43681 cgtttttcgg tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg
    43741 cgacatcaaa aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg
    43801 cgctggcaat cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg
    43861 ccgccagcgc cgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg
    43921 tgccgtgagc atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc
    43981 cccgacgttg taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt
    44041 ccggctcacc gacaattgcc ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa
    44101 tactttcgcg atctggaccc agccagtcgg tatgatccag cgcaatactg gttactaccg
    44161 cgacatcggc atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa
    44221 tcaccacgtc aagttgtgcc tgcttgaaca gccacaacgc cgacagcgta ccgtactcga
    44281 aataggtcag ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg
    44341 ccgattccgg caattcctgg ccctgcacac gtacgcgctc ggtataacgc accagatgag
    44401 gcgaactgta gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc
    44461 aggtggtgcc tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagga
    44521 cgccaagacg cgccgcgacc tggctcacgc gctcaaggcc gagatcgata gttttactgt
    44581 gcaggttttc cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt
    44641 tgataatcat ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg
    44701 gccctgccct tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta
    44761 cgccttcacg cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc
    44821 tcgccagttt caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga
    44881 tcaggaattc actgcgctgg aatctaggcg gcagtttttc gcgaacggtc tgttcgataa
    44941 cacgcggacc ggcaaagccg attaacgctt taggttcagc gatgttgaga tcgcccagca
    45001 tggcgaagct tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca
    45061 agccgcgctc ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca
    45121 tcagtgcttc ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat
    45181 cttcctgcgc ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac
    45241 cgcccataaa ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt
    45301 tcatcaccac cagcgcatct ttttcgccgg tttctttctg cgcagatgcc agacggtctt
    45361 tatacttctt ggagtcacga aacttcagct catctttcgg ctcaagctcg ctacccagct
    45421 ccacaaggct tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt
    45481 gatggtcaca cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct
    45541 gaccgcagct atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag
    45601 taatgttgct tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt
    45661 tcgatgccag ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga
    45721 atgttgcaca ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg
    45781 agttactttt tattttgcgg cgcgtgccgc agcgcgtttg tgacggatta tttcgatgac
    45841 gcccggcaaa atagaaacca caataatccc gacgatcagc agcttaaggt tatcctgaac
    45901 catcgggatt gtaccgaaga aatagcctgc gtaggtaaaa agcagtaccc acaacagtgc
    45961 gccgatcacg ttataagcag cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa
    46021 cggggcgaac gttctgacga tcggcacaaa acgggcgaga ataatcgttt tgccgccatg
    46081 tttctcataa aactgatggg ttttgtcgag ataactgcga cggaaaattt tcgaattggg
    46141 attactgaat aacttttcac cgaacaaccg cccgatagtg taattgaccg cgtcacccac
    46201 aatcgcggcg atcagcatca gcactaccat catatggacg ttgagatcgt tggtttccag
    46261 tgatgccaac gctccggcaa caaacagcaa tgaatcaccc ggtaaaaacg gcattaccac
    46321 cagaccggtt tcacagaaca aaattaagaa caaaatggca taaacccaga cgccgtactc
    46381 cgcgactagc tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat
    46441 caggtccata tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct
    46501 acagcctgaa atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg
    46561 gcggttttgg aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg
    46621 cttttgccgt tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc
    46681 tctccggctg gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat
    46741 gatgtacaaa ggcattcgct ttgatatcaa ccaccacata aggaccgtga cgcgtgacat
    46801 taatatgcat aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg
    46861 tgaaatcatt ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg
    46921 gttcgtaaaa atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga
    46981 tgatgtagcg ataacggcga gccgtggcgc taaatcgtgc atgaaaatca tcaggtacag
    47041 ctttaaccca acgcacggcg atgtcaccag gtaaattcgc atttacgccc aaggtccacg
    47101 ccgcgtcttt gcgctgcgcg gttgtttcga aatgcaccac ttgcccggta ccgtgtaccc
    47161 ctgcgtcagt acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg
    47221 ccttttccag cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact
    47281 tactgccgtc gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg
    47341 acattagtac agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc
    47401 gccaaagcga acgttatcgg ccaccgacca gaactggact tgctccggca taccgtagtc
    47461 attacgcacg cagccaacag aaagatgcgg cgtacccgaa gcatccccta cctgagtcgg
    47521 gaattcgttc tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc
    47581 ttctgccgcc agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac
    47641 cggtgcctgg acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac
    47701 ttcgtcaacg atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag
    47761 catattgaac gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag
    47821 caatttcgca ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga
    47881 aatcaggctg gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc
    47941 cgccagcagc tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc
    48001 ggtcagtaca aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa
    48061 cagtccgctg ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc
    48121 ggtagcttct ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc
    48181 ggcatcctgc acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt
    48241 acgtgccagt gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag
    48301 ggcttcgccc atagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt
    48361 ggtttactcc agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga
    48421 gattaacgtg ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca
    48481 ctggcgtcat cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg
    48541 cgcagtttat cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg
    48601 cgcacatcat acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cggaccatgc
    48661 agcgtaatgc ggccaaactc aggcgcgggc agcaacgtat ccagcgcaac gtgttgttca
    48721 tgcccaataa acttgctata ggcttcaaac acttgcgtcg tgccacgcgc tttaccctcc
    48781 agggtatagc ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ttccacatta
    48841 agttccggct cgccttccca gacatccagc accacgctta acttctggcc ttcattcagg
    48901 caggtcagca gcgcggtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct
    48961 ccgggcttca ggctacggat cagtttttca tccgccagat gtagcgtttt gtacggacca
    49021 tctttaaaga gtggcgtatg gaaagtcaga atatccgcgc gctggactaa ctcatccagc
    49081 gagcggaaat caccctcatc cccacggtcg gcgcgaggcg gatcgcaaag taaggtttta
    49141 atccctaacg cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc
    49201 cccaccgtac ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag cagggaggag
    49261 aaaacatatt ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc
    49321 gcctgcttca accatgcttc atcgacatgg tcggtccccg cagtggcagt tccaacaaat
    49381 ttaatgggtt ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc
    49441 gcatccgcgt ctgccagttg agcgacgggg attggacgcc cgggaaccgc gatcacctca
    49501 cccaaacggc taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg
    49561 tttgtgttac ctgtatgaga cgagagttaa ccggacaagt atgccataat ctcgcggcca
    49621 ggcatacttg cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc
    49681 tcctcctggg cagccaccag gtcagggaga taacctgccg tctggcgcgg gcaatcagcc
    49741 tttatccagt cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac
    49801 gcaacaacaa agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga
    49861 ttcaccgctg ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct
    49921 ggccgcgcaa aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg
    49981 tctggggaat catcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc
    50041 gctgaatcag ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa
    50101 gatggttatt ccgcaaccac agcagcgcga ggcgaccgac cgtcctttat taccggcgga
    50161 gcacaatgcg ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa
    50221 acagatctgg caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc
    50281 gaaactgttt aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa
    50341 tacgccgacg ctggaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact
    50401 ggcggcgtta tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc
    50461 ttctgcccag gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga
    50521 tccgcgtgat atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa
    50581 tatggcaacg cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg
    50641 gctggttcgt taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga
    50701 gatcgccccg gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag
    50761 cggcccggag acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc
    50821 gcgaacttgt gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga
    50881 acatccggct ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca
    50941 gagcagcaac aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc
    51001 aaaacggtcc ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc
    51061 ggtaagagta aagcccgcca tcgcccatcc tttgctggca aagtagagcg aaacgaaagt
    51121 cccgataacc gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag
    51181 cccgacaacg ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt
    51241 gccgttacag gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa
    51301 accgtaatgg ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat
    51361 cgccattccg ttccatgaca tcactttgcc agagtgtttt ggccctacga tgcctaaccc
    51421 ccaggtcaga gcgcctgtca gtaactggct ttcaccaaac ccaagaatca aacgcccgat
    51481 gaccaacagg gcaaatttga acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc
    51541 gccgccagcc agaccacaag ctaacatccc ctgaagcgcc gaacgttttg cgccatattg
    51601 atcggccagt cgcccggcgt aaccacgcgt cagcaccgta gccagaaact gaatcccgac
    51661 ggcaatgccg accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac
    51721 cggcaacggc aaccctacgg tcatgtaggt gagaaaaacc gcaaaagaga tgcggaagag
    51781 cgaaaaactg gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag
    51841 aatgcagcgc aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacggtgc
    51901 gttggcagga tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc
    51961 ctagaactgg tggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg
    52021 ttagtgccgg aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg
    52081 acctactccg gcctactgcg aattaatctt ttcagcttgc gcattaccag cgtggcgttg
    52141 gtgccgccga agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg
    52201 gtcacgatgt tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg
    52261 ataaagccgt gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc
    52321 agtgagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact
    52381 tcacggatag ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc
    52441 aggtaatcga ttggggtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct
    52501 tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca
    52561 gcatagatgt aagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg
    52621 ccgccgccag cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc
    52681 ggggtgtcgt tgtatttagt agatagcgca cccatcgcgt cgaattcgca ggccatttcc
    52741 cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc
    52801 tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta
    52861 acgccatgaa ttttaaacgg cgtggcgaga caggcagaaa cgccggatgc catcgcttta
    52921 gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg
    52981 aacacctgga aacgcgggga accgccgccg gaacctgcaa tcaggccaac gcgcgggtta
    53041 ttctggtaag cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat
    53101 gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta
    53161 tccagtttta tgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag
    53221 aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg
    53281 atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag
    53341 tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg
    53401 atcagccatt taatagagat atttgcgcag ccttacacac atcgctaaga tcgagccacc
    53461 gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt
    53521 taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa
    53581 cgggctggaa gagacgcgtt atgtttttct gggaggtaac caattagagg tacgctttcc
    53641 tgagcatcca catcctctgt ttgtgatagc agagagcggc ttcggcaccg gattaaactt
    53701 cctgacgcta tggaaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca
    53761 acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc
    53821 gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat
    53881 gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg
    53941 gtttggcgat attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga
    54001 tgcctggttt ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct
    54061 gtttaacgcc atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc
    54121 cggttttgtc cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt
    54181 tgggcgcaaa cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc
    54241 cacgccgtgg tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg
    54301 tattgccagc gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta
    54361 ttgcgcggat gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc
    54421 gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc
    54481 tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca
    54541 gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc
    54601 cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac
    54661 aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg
    54721 taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca
    54781 gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac
    54841 acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac
    54901 tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc
    54961 agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca
    55021 acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga
    55081 cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa
    55141 agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca
    55201 tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt
    55261 ggcggagcaa aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc
    55321 ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca
    55381 gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg
    55441 gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat ctgctctcgc
    55501 agtgtttgcc ggatgcggcg tgaacgcctt atccaaccta cgtgtgacgt gtaggcctga
    55561 taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tacagctgtg
    55621 acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca
    55681 attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag
    55741 cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat
    55801 acccactggc aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca
    55861 gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctgac
    55921 ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat
    55981 aattttgtag tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat
    56041 ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg aaatcccctt
    56101 ccagattgtc gcaactgacg ttaaacggat atccagccgc cacgcagaat aaccagtcga
    56161 acgcctgcgg cttcacttca acatcttcaa actggctttg cgtctgggca tcgcgtccgt
    56221 ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc
    56281 agtgcgaaat ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat
    56341 acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg
    56401 tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctcg tagtggtgtg
    56461 tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag
    56521 taatttggca ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc
    56581 tttgctcaac accaaccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac
    56641 aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt
    56701 tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag
    56761 cataacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa
    56821 accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc
    56881 ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg
    56941 caaaatctgc cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga
    57001 gccgacaaag gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg
    57061 aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag
    57121 cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc
    57181 accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa
    57241 cagcggagaa accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt
    57301 agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc
    57361 ggttttggag gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt
    57421 aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc
    57481 gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca
    57541 aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg
    57601 gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta
    57661 ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga
    57721 gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg
    57781 ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc
    57841 agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa
    57901 tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca
    57961 gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt
    58021 tgggttattt tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc
    58081 agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca
    58141 ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg
    58201 atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact
    58261 tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc
    58321 gccatatgag caatgatttg ctgcccgctg ctgataccgc cgaaaatgcc gcccgcatgg
    58381 ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact
    58441 acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg
    58501 gcgatgtcgg catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg
    58561 gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt
    58621 tcatctaacg cgtcgatttt gtcggggtcc gggcagaaga acggattttg ttcaaccagc
    58681 gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca
    58741 ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg
    58801 gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg
    58861 taggtgtaat cagcatggcc tggacggaag acgtctttaa ttgcgctgta atcctgagaa
    58921 cgctggtcgg tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa
    58981 acaccggaga gtattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc
    59041 ccagggcgac gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc
    59101 ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg
    59161 cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt
    59221 gtttacttaa tctttataaa tcgcgaaatg ttctcgtgcg gcaataagct gctctttagt
    59281 gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatcggg
    59341 atattgttcc ataagatgta ccatgctgtt gcccacttca caaatcaaca cgccatcatc
    59401 agcaaggtaa tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc
    59461 agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc
    59521 cgcgtcgaca tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg
    59581 gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg
    59641 ttcggcaacc gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa
    59701 ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg
    59761 cggttgcttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg
    59821 cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt
    59881 caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg
    59941 tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta
    60001 gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta
    60061 ccagatattt gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg
    60121 cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc
    60181 gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg
    60241 tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg
    60301 aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag
    60361 gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac
    60421 cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct
    60481 gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg
    60541 aaatatgttc gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat
    60601 tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg
    60661 ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg
    60721 cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg
    60781 atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt
    60841 gaagtggaag aatggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca
    60901 gcgtaaacgc cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc
    60961 gtaggcctga taagacgcat cagagcaata ctacgaactt cagttcattc aatacatact
    61021 gaaaacgaca cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat
    61081 cgtgtagtac tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat
    61141 cacaaccgta agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc
    61201 catcattatc agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg
    61261 aaagcgtggt ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga
    61321 cataattttc actccaggag atatcgtatg aagcgaccgg tatcgttggg catattgtgt
    61381 tatgcactgt aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg
    61441 atgcgatcca agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg
    61501 cacaacgaaa aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt
    61561 cagtcagtac caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat
    61621 cgcctttata cgcaaaagaa tcatatttcc catatgcaac ctcctttggc gttatcaccg
    61681 taacattgaa aacactgctc gctacagcac cggttacctt gtccatggca taataaactc
    61741 tcacactgtc aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga
    61801 caacactcag aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag
    61861 aaatactcaa aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg
    61921 ctctttgcct gtaaccaggt attaaaattt aggctgttat ttccctgatt cagcgggagc
    61981 gtgacgcctg ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc
    62041 ccagcgccgg atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct
    62101 tctgttcctg tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat
    62161 tgaaacacca atggcactcg ctctgactgc ccaaggttca ttaaatcact ggcggcaatg
    62221 gcaggaaaat gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg
    62281 attaaattgc cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac
    62341 atcaggctac taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg
    62401 tggcccaggc ctcaaagtcc gcctctggca gaatgacacc actctttttt actggcacag
    62461 ccgttaacac cggtaaatcg gtttcattca ccacaagtgg cgtattgatc gtaaaactct
    62521 gccctgcctg tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat
    62581 caaaatcgct tggtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg
    62641 ccgtatcgca ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat
    62701 caataagcac atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag
    62761 gcggctgcac taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc
    62821 acagcaaaat aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt
    62881 aaattcagct gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg
    62941 aactcccgag gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg
    63001 atcactactt ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc
    63061 accgttaatc acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt
    63121 gttacaaaca agcgagtagt caatgggacg tttataatca accctattga tcttattaat
    63181 gaatacatta ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac
    63241 aagcacccgt aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac
    63301 acaacatatc agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct
    63361 cccggcctca gcctttgcgg ctctgctctt catcgaccta gcaactgtta ccgttgcaag
    63421 tgaaaaagag cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac
    63481 ccatcttcac attcagcggt gcagaggttt tcggcgacat cactattagc gagaaaccgg
    63541 ctgccggatt gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacgacgtcg
    63601 ggttattgac ggtaaattcc tgaccgttgc gggttaaagt aatcttatat tgctaggggt
    63661 ttttcatgct gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta
    63721 gtgctatttg cagcgtgttg gctttgtttg attttggcgg gatctcgcgc acgttgaagt
    63781 agaacaggct ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta
    63841 cctgcccgtt catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac
    63901 tgatcttgtt acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat
    63961 cgttattgcg caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac
    64021 gatctggcgt tacagaagcc tgggcagaca aactggcagc gctaagcagt aacgctagcg
    64081 tcgtagcacc caatttggca gagcaaagta aatcagacat ggatcattcc ttaatcacgt
    64141 tattggttag ctgaaacaga aacaggggga ttggtgggtg tcacctgttg cgtctgctcc
    64201 tgaatgacag gctttatgtc atcaggaaca accggcgcga cgttgccttt atgctggcat
    64261 ggcagtaaca agccgttaaa cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa
    64321 tgcgcaacac cactccagaa cacactcatg tgttcgccag gtttcacccc agccagataa
    64381 acattgccgt catcatcgac aaggcccact gtctgctcgt tatcattttt tacttctgcg
    64441 ccaaacggag gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg
    64501 atgacggcaa atttgcggta gccaatcgcc ccttcagtta gcgtggcttg taccaccgac
    64561 tgggttgctt cggcgttttc cggcagtttg ttgaggtcga tatacgcctg attgcggtaa
    64621 tagttattga cgtcagaaac gacggcttta ccaaacatat tggtataaac agcagcaccg
    64681 ttaccttcta ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc
    64741 atattctggg tacggtgaag tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac
    64801 gacaagcccg cagaggtgta ttgcccttca tggtagttcg cactgaggtc aacctgcgcc
    64861 agcgaaccat catggctgta atagccgtca acgctggtgt gtttgtcact ggtgccgacg
    64921 ttcaactgat agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg actgctgtcc
    64981 gtcccactgc catagtaccg ttatagctaa cggtactgtt gtcgccccac ggcatactga
    65041 gcgaaatgta catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg
    65101 agatgctgac attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt
    65161 ttgtctgctc ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac
    65221 ccgcatcgcg gaagttctgg ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc
    65281 gtaccattcc gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg
    65341 agaagcgata tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac
    65401 tcacacggaa ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt
    65461 tggtgtgcga gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga
    65521 caccaagcgc cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc
    65581 cgttagcgat cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc
    65641 cccactcttg cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt
    65701 atggcatcga ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc
    65761 gaatatgcaa cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg
    65821 gaacctgcgt ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt
    65881 gtgccacgcc ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt
    65941 cagtactgac actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat
    66001 cctcgcccag cgccagtttg gcttttaatg atggtaacgc ccgcctggca tagtagcgac
    66061 tccactccca ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag
    66121 tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccctagg ttaaccccaa
    66181 ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt
    66241 gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg
    66301 gatcccagtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg
    66361 actggcttaa atcagcctta atttccatgc cttccagttg accggatttc aggcatttag
    66421 catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca
    66481 gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa
    66541 tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg
    66601 gctcaacata tccctagctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt
    66661 ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg
    66721 acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca
    66781 tacacgtttc tatccgtttt tattcttgtt cggtcaaaat acatttaaat cccccatagc
    66841 agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcaa
    66901 aattacccag atctggcgca tcagctgcgc cgaccagcca ggcgttaaag ttcagtgtct
    66961 gcttcgcttt gcctttgttg gtagaagtgt ctttcactat tttctggtca atacccatgc
    67021 cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg
    67081 caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac
    67141 cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct
    67201 ggaaactttt cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca
    67261 tatcaacttc cagtttgtga tcgtctggaa cgatagaaca cactgagtct tcaatggtgc
    67321 cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc
    67381 ccatcaccat tgccgcagca atagctgttt taacaaactt actcattatt tgtccttaaa
    67441 ctataaataa ttaaaaatat aaacattgca atacattgat tcagtcaata gccaatattt
    67501 atacccagcc attgaatacg gcatgcgacc caaaaagaac aggtcgtaga aaattgtatt
    67561 cattggtgtg gggccgcata gtgatgtttc cacacccctg atccaattta attttactaa
    67621 agagtaaatg tagatttaat taatatattg acagaagaaa ggttatctta aggacatcct
    67681 taaaaaatac ggaataagta tttagaaaaa atagaaagta tatcaataat gaatatcaac
    67741 agcttaaaaa acaaacaatg aacatataat gcgattggca tttatccact tttgacaata
    67801 taatatataa acactatagc cagaatttaa tcaagtttca attacatcct gcctgattat
    67861 tcaactctat tttctctatt atctgtatta ataaatgaca atgagtgtca aacagatatt
    67921 attcatttca acatgtaact ttgggaaaaa tcaagatacg cgcagaagaa taatttccga
    67981 atgaatgtag aaagtgtttt atttatagat aaggagagtc ttcgatagta gtgaggttga
    68041 cagatatccg ctgcctaatg agcaattatg cccaatgaat ctacctcatt gggcataatt
    68101 tggaacacat cagatagctt ttgccatctt cagattacac gggctcatct gccagttgaa
    68161 tgtaccgttt ccgctttcat caagcgttac gctggcaatg gccgaggtgg taaacatagg
    68221 cggcgtttcg cccggacata actcggcaac cagatacccg actaacggta agtgagagat
    68281 caccagtacc gaagcaacac cttcattggt caacgcctgc aaataggcac tgaccagacc
    68341 aacatcgccg cagggcgtta actccggcag aacttctgca ctggaaggca ggttcagaca
    68401 atcccctact tcttccagtg tttgctcggc tcgcaggaac gggctcacca gaacgcgttc
    68461 gatttccact ttttgacctt tcagccagtt cgccatcagg cgagattcgt cacaaccatt
    68521 agtggtcaga ggacgaaccg aatcactggc ggaatcgagg gctgcgtcgc cgtgacgcat
    68581 gataaaaact tgcatattgc accgcttttg ttaaccagat tcgcccgttt tctttcatct
    68641 gaaaccagag aaagaaaacg gtggccggca ttgtgcctta tccattcacc gaatgaaacg
    68701 ctgtttttta cctcaatggc gtaagtatag tcaatatgcg tttacatttt gaccaacact
    68761 ccgccattca gcgcggattc atatagcttt gaccttctta ttgcaggtca gttgcagttg
    68821 ttttccaaaa actttcccca cgcgcgccca tctcgaccaa acgctcgcaa ggggtaaaac
    68881 gggaaccata ctgcgtggcc agtcgttgca ttattgcaac cacttcgccc gcgccgagag
    68941 aatcgatata gcggaacggt ccaccgagaa atggcggaaa accaatgcca aataccgcac
    69001 caatatcccc gtcacgcacg ctacggataa cctgctcatc aacacaacgt actgcttcat
    69061 tcagcatcaa catcacacac cgttcagcaa cctgcggtgc ggagagtcac ccctgccctt
    69121 gtgcgccaat gagcgggtaa atggcaggat cgacctgttt tttgctttta cgccctttct
    69181 gaccataaag atagaaaccc cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg
    69241 aagaaacaac atttgcaggc gcgctaaaac gttctccata agcggcttcc agtacaggaa
    69301 taattttagt cccggtgtcg attcctacct catccaaaag ttggattggg cctaccggaa
    69361 aaccaaattt cactagcgcg gcatcaatgt gctctacccg ttcaccttcg gtcagcatgc
    69421 ggatagcttc attaatgtaa ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac
    69481 gcacgacaat gggcgttttg ccctgttttt tcgccagttt taccgtagtg gcgatggttt
    69541 gcgccgatgt acccgcatga ggaataatct ctaccagcgg cattttttcc accggactga
    69601 agaaatgcag gccaataact tgctcaggtc gcgcggcgtg agcggcgata tcaccaatcg
    69661 gtaaagatga cgtgttcgaa gcaaaaatgg tatgagcggc gcaattttgc tccacttccg
    69721 ccaccatctg ttgttttaat tcgagatttt caaacaccgc ttcaataatc agatcgcgat
    69781 gggcaaagcc gcgatagtcc gtcgttccgg agattaatgc cagctgtttg tcacgttcgc
    69841 tggctttgag atgacgacgg cgaactttgc cctccagctg atcccaactg tacttcagcg
    69901 catgatttat gccctgcggg ttgatatctt taattctgac cggaagcccc gctttacaag
    69961 cagtgacata agcaataccg ccgcccatca agccaccacc taaaattccc acgctgttta
    70021 atgccgcagg cggtgcatca ctaccgggat ctttcttcac gtccgtactg gcaaaaaaga
    70081 tactacgcaa agcttgcgat tgcggcgtca tcgccagttc gccaaaggcg cgagcttcag
    70141 cgtcatagcc gctgctggtg ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt
    70201 ctgtcgccgg ataattgcct tgagttttgt gttctgtttt cttgccgacc attttgaaca
    70261 gcagcgcacg acctaacggc cccgccagaa tacgctcgcg tacaggtaga gggcgggaag
    70321 atgggcgatc cttcttcgcc agctcaacag cggcttccag caaaatggag tgcggaacaa
    70381 cgtcatccac cagccccagc tttaatgcct gtttcgcccg aagttgtttt ccggtgagaa
    70441 tcatctctaa tgctgtactg acgcctatca gacgcggtaa acgctgggtg ccgcctgaac
    70501 cgggtaacaa tccaagttgt acttcagaca aaccgagcac cgttttagga tcgtcagtac
    70561 aaacgcgacc gtggcacgcc agcgccagct ccagcccacc gcccaggcaa gcgccatgaa
    70621 tagccgcgat aaccggaatg ggcaaagcat gaatctccgc catcaactgt tggccctgcc
    70681 gcgccagtgc ttccgcttct tgcgccgttt tgcagttgcc aatcatgttg atgtctgctc
    70741 cggcgataaa attgtccggt ttcgcggaga taaacaccac gccgcgcaac tctttgtttt
    70801 cacggagttg cttaataatg gcgcgcacct gcgaggcaaa ttccgccttc agggtattca
    70861 ttttctcatc cggtacgtcg atggtgatga tagcaatgtt gtccagacga acattaagag
    70921 taaacgctga tgccatttcc attattccgc ctccagaacc attgccgcgc caagcccacc
    70981 ggcagcacag gcggtaacta aaccaaatcc accgccgcga cggcgaagtt catgcaatgt
    71041 ctgggtaatc atccgcgcgc cggtcgccgc gaagggatgt ccgtaagcaa tcgaaccgcc
    71101 aagcacgtta aatttgctat cgtccacttc gccagtggca tgtgcacgcc ccagtacttc
    71161 acgagcaaaa cgttcactac ccagcaactg aatattcgcc agtgtctgag ctgcaaaggc
    71221 ttcgtgcata tcgatcaatg tcagttcgct catcgtcaaa ccggcacgtt ccagcgccag
    71281 cggtgttgac caggctggac cgagcaacat gtcctgccag acatcaatcg cagtaaatgc
    71341 gtagctgcgc agatacccca gcggcaccag ccctaattct ttcgcccggg attcagtcat
    71401 caggatcacc gctgccgcgc catcggtcag cggcgtactg tttgccgccg ttaccgttcc
    71461 gtgtttgcga tcaaacgccg ggcgcagctt tgcgtaatcg gcaagcgagg aattaccgcg
    71521 aatattgttg tcttcgacaa gcggttgttt ataaggaggg ataaaggcag tcatcacctc
    71581 ttctttgagt tttccgtctg accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa
    71641 tgcatcttgc tgttctcggg tgatgccgta ggttttcgcc atttgttcgg cggtgtcgcc
    71701 catccgcaag ccagtagaat attctgctac cgcaggcggt acgggcatta agtcgcgcaa
    71761 acgcaggcga gagaagagtt tcaggcgctg gctcatggtt cgagctttgt tgacatcaac
    71821 cagcacgtgc gccagttttt tactgacgcc aattggcaat acggaagagg aatctgcccc
    71881 accggcaatc cccgctcgga tagttcccgc catcaggctt tctgcgacgt ttgcaaccgc
    71941 ctggaaactg gtagcgcaag cgcggctgac gctgtaagca tcggtatgca cattcattcc
    72001 ggtaccgaga acaatttcac gcgcaatgtt gggggcttca ggcatttgta cgacctgacc
    72061 aaagaccagc tgttcaatca cttcagcggg gatctcgctg cgtgccagca gttcgcctac
    72121 caccatcttc cctaaatcaa ccgtgggaat gccatgaaaa gccgtcgcct ggcgggcaaa
    72181 aggcgtacgt aaaccgctaa caatggcgat acgatcgccc tggcgggtaa ccagtggtaa
    72241 aacctgaccc ataacactcc cctgtaaaaa aataaataaa gtggtctgac ctgatcatag
    72301 tcttaaccat ttttttacat ttagccaagc ggagaaaaag gaaagtggga gctatgacac
    72361 agtgaagaca gaagaaagaa aagaaaacgc ccctgccagc aactgacagg ggcgtacgta
    72421 cccgagagga attaacgcag acccagctgg aagatcagca tttcagcttc gcaggcaaaa
    72481 gtgaaatcga tatccaggcg cacaccgtca gactcttcag tgaaagtcgg ggagattttg
    72541 cacggttcgg attccacgct acgggatttt tcagtcagcg ccgccagcgt ttgttctgct
    72601 tctgcgcggt ttgcaaacac gcggctgtaa gacgcggtgc agtcggagtt gtccataatg
    72661 gtgccaacat ccatacagca gcaaaccggg gtttcatcag cactacattt actcatcgtt
    72721 gatttcctct gtatgtgcac ccaaggtgcc agataaacgt tgtggatatt ttacgcttcc
    72781 ggaaagtgct gctccagttg ttaattctgc aaaatcggat aagtgaccga aatcacactt
    72841 aaaaatgatc taaaacaaaa ttcacccgaa tccgcgagtg cgcaacctcc aaattttgcc
    72901 agctggatcg cgtttcttag atcatatttg aaaaaagatg gaaacatact tgcaacattc
    72961 tagctggtcc gacctatact ctcgccactg gtctgatttc taagatgtac ctcagaccct
    73021 acacttcgcg ctcctgttac agcacgtaac atagtttgta taaaaataaa tcattgaggt
    73081 tatggtcatg agccagaaaa ccctgtttac aaagtctgct ctcgcagtcg cagtggcact
    73141 tatctccacc caggcctggt cggcaggctt tcagttaaac gaattttctt cctctggcct
    73201 gggccgggct tattcagggg aaggcgcaat tgccgatgat gcaggtaacg ttagccgtaa
    73261 ccccgcattg attaccatgt ttgaccgccc gacattttct gcgggtgcgg tttatattga
    73321 cccggatgtg aatatcagcg gaacgtctcc gtctggtcgt agcctgaaag ccgataacat
    73381 cgcgcctacg gcatgggttc cgaacatgca ctttgttgca ccgattaacg accaatttgg
    73441 ttggggcgct tctattacct ctaactatgg tctggcaaca gagtttaatg atacttatgc
    73501 aggcggctct gtcgggggta caactgacct tgaaaccatg aacctgaact taagcggtgc
    73561 gtatcgctta aataatgcat ggagctttgg tcttggtttc aacgccatct acgctcgcgc
    73621 gaaaattgaa cgtttcgcag gcgatctggg gcagttggtt gctggtcaga ttatgcaatc
    73681 tcctgccggg caaactcctc aagggcaagc attggcagct accgccaacg gtatcgacag
    73741 taataccaaa atcgctcatc tgaacggtaa ccagtggggc tttggctgga acgccggaat
    73801 cctgtatgaa ctggataaaa ataaccgcta tgcactgacc taccgttctg aagtgaaaat
    73861 tgacttcaaa ggtaactaca gcagcgatct taatcgtgcg tttaataact acggtttgcc
    73921 aattcctacc gcgacaggtg gcgcaacgca atcgggttat ctgacgctga acctgcctga
    73981 aatgtgggaa gtgtcaggtt ataaccgtgt tgatccacag tgggcgattc actatagcct
    74041 ggcttacacc agctggagtc agttccagca gctgaaagcg acctcaacca gtggcgacac
    74101 gctgttccag aaacatgaag gctttaaaga tgcttaccgc atcgcgttgg gtaccactta
    74161 ttactacgat gataactgga ccttccgtac cggtatcgcc tttgatgaca gcccagttcc
    74221 ggctcagaat cgttctatct ccattccgga ccaggaccgt ttctggctga gtgcaggtac
    74281 gacttacgca tttaataaag atgcttcagt cgacgttggt gtttcttata tgcacggtca
    74341 gagcgtgaaa attaacgaag gcccatacca gttcgagtct gaaggtaaag cctggctgtt
    74401 cggtactaac tttaactacg cgttctgata acgcgttcgc ctagataaag tcacctgcta
    74461 tgcaggtgac tttaactccc ccacttcacc gaaagtagtt tcctcattgc ttgcctcctg
    74521 agttttgcaa acagcctgat ggcagcttgc cctttcaaat cagtaagtag tatccacaga
    74581 caaacctgaa gggaaatgca tattttcaag cgttctgctc gtccttctca aagagtttac
    74641 ttttctgcat ttccaggata ctcaaccccc ctggctattg tgcgcttata cactcaaatt
    74701 aaagataggt tctaaataaa tgagcgtttt ttgatagtct atttcattag gtaatatata
    74761 tttgtaacaa atcaatcaac atggaataaa atcatgctac catctatttc aatcaacaat
    74821 accagcgcag cttacccaga atccatcaat gaaaataaca atgatgaaat taatggatta
    74881 gtacaagagt tcaaaaacca tttttaatgg taaggaagga ataagcacct gtattaaaca
    74941 tctacttgag cttataaaaa acgccatacg agtaaacgac gatccttata gatctaatat
    75001 taataattcc tcagttactt atattgatat tggctccaat gatacagacc atattactat
    75061 tggtatcgac aaccaagaac caatagaatt acctgcgaac tataaagaca aagaactcgt
    75121 ccgtactatc attaatgaca acatagttga gaagactcat gatatcaata acaaggaaat
    75181 gatcttcagc gcattaaaag aaatatatga tggagatcct ggttttatct tcgataagat
    75241 atcacacaaa ctcagacata cggtaacgga atttgatgag agcgggaaaa gcgaaccaac
    75301 ggacttattt acctggtacg gtaaagataa aaaaggcgac tctctcgcta ttgtaattaa
    75361 aaataaaaac ggaaatgatt acttatttct cggttactac gatcaggacg actaccacat
    75421 tcaaagagga attcgtatta atggtgatag tctcacccaa tattgtagtg aaaacgccag
    75481 gagtgcttca gcgtggtttg aaagcagtaa agctatcatg gcagaatcat ttgcaactgg
    75541 ttccgatcat caggttgtaa acgagctcaa cggggaaaga ctgagagaac ctaacgaagt
    75601 ttttaaacgt ttaggtcgag caataagata taattttcaa gtggacgatg caaaatttag
    75661 acgcgataat gtaaaagaaa taatttctac tttattcgct aacaaagttg acgttgacca
    75721 tcctgaaaat aaatacaaag actttaagaa tctcgaagat aaagttgaaa aaagacttca
    75781 aaatcgccag acgaaatatc aaaatgaaat taatcaacta tctgcactag gtgttaattt
    75841 tgatgatatt taaaattatt tttccagttt tttgattttt caacgcctga atatcggaaa
    75901 tagtcagtga caaacatcag tatttttaca tcattacgtc cgggtcattg tcggtttatc
    75961 tccttttact tgtggtaatt gagggataac aacaaaaaag gtgagttttg cgactcacct
    76021 tttttatttg tttcttattc agaatcgata tcttttaaat catcctgaat cgcttgcgcg
    76081 ttcggatttt cctgcggttt gagttcgccg ccattagcga tgaaatcatg acgctggaag
    76141 tacgcttcgc gcaccataat ataaggatcg gacgactgac gcagcagacc atcggaatcc
    76201 agcagctgag cgcgggtttc gatcccttca agcgtccatt tacccacaga catcggccag
    76261 gtcagccagg aaagaaccgg gtaaagagca tccgccatat caccaccgtc atcacgcagc
    76321 gtgaagctac cgtagaacgg taactgaacg taaggcccat aacccacgcc ataatgacca
    76381 agcgtactac cgaagcggtg aggttcagtc cgttgcagtt tcgggttcgc catccctgca
    76441 acatcaataa agccgcccat ccccaaaatg gtgttcagga aaaagcgggt aaagtggacc
    76501 atcccctgat aagggtcgcc ctgcaagaag tagttaacca tcaccgcagg ttcttcaagg
    76561 ttgccagtaa agttgctcaa accattacgc gccggttgcg gaacataatc acgccaggcg
    76621 acagcgaccg gtcgaacaat atacgggtct aatacgttga agttgaagtt gtacatggtg
    76681 cggttgaacc cttctaacgg gtcagaacgc ccttgctgat ctgtaccgga actcgcacac
    76741 cccaccagaa gcgtagttcc cagagcaagc gccgacaggc gaagcttcat aaatgtctcc
    76801 ctgttttttt atggcttatg cagtttgcca tccatgacgg aacgataccg tatccgcctg
    76861 tttaggtgtg ggcgattgta acagcacgtc aactgatgtc cagacgccct gatttgctga
    76921 tttgatcata gcctggtaat cgccgccctg taggctactt gattctatag aaacagaaaa
    76981 aggcaaacgc agccttttct acattttcag agtaactccc gccagttgcg agcaaaaaag
    77041 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
    77101 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
    77161 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
    77221 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
    77281 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgccg aactggaagg
    77341 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
    77401 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
    77461 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
    77521 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
    77581 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
    77641 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
    77701 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
    77761 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
    77821 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
    77881 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
    77941 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
    78001 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
    78061 agtccattag cgggtatact cgccgcattg tcctcttagt taaatggata taacgagccc
    78121 ctcctaaggg ctaattgcag gttcgattcc tgcaggggac accatttatc agttcgctcc
    78181 catccgtacc agtccgcaaa atcccctgaa tatcaagcct tccgtatatt cacagttcgt
    78241 catggttcgc gtcagatcgt tgacagccgc actccatgac gggtaaaaag tggataaaat
    78301 aattttaccc accggatttt tacccatgct caccgttaag cagattgaag cagcaaagcc
    78361 gaaagaaaaa ccataccgcc ttctcgatgg taatggcctg tacctttatg tccctgtatc
    78421 cgggaaaaag gtatggcagc ttcgctacaa gattgacggt aaggagaaaa tcctgaccgt
    78481 cggaaaatat ccgcttatga ctttgcagga ggcaagggat aaagcatgga ctgcgaggaa
    78541 agacatctcg gctggcatcg atccggtaaa ggcgaaaaag gcttcgtcta acaacaattc
    78601 atttagtgcg atttacaagg aatggtacga acacaagaag caagtctggt cagtagggta
    78661 tgcgactgaa cttgcaaaaa tgtttgatga cgacatttta cccatcatcg gcggccttga
    78721 gattcaggat attgagccga tgcaactgct ggaagtaatc cgcagatttg aagatcgcgg
    78781 tgca
//
LOCUS       DAJVRP010000003        72896 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVRP010000003 DAJVRP010000000
VERSION     DAJVRP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.003"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQN12_000165"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQN12_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1652642.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQN12_000166"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQN12_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1652643.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQN12_000167"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQN12_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1652644.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQN12_000168"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQN12_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1652645.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQN12_000169"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQN12_000169"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1652646.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQN12_000170"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQN12_000170"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1652647.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQN12_000171"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQN12_000171"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1652648.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OQN12_000172"
     CDS             complement(4598..5320)
                     /locus_tag="OQN12_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1652649.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQN12_000173"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQN12_000173"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1652650.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OQN12_000174"
     CDS             6520..6738
                     /locus_tag="OQN12_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1652651.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQN12_000175"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQN12_000175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1652652.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OQN12_000176"
     CDS             complement(7472..7672)
                     /locus_tag="OQN12_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1652653.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OQN12_000177"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OQN12_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OQN12_000178"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OQN12_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1652654.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OQN12_000179"
     CDS             10101..12014
                     /locus_tag="OQN12_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1652655.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OQN12_000180"
     CDS             12014..13036
                     /locus_tag="OQN12_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1652656.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OQN12_000181"
     CDS             13030..13248
                     /locus_tag="OQN12_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1652657.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OQN12_000182"
     CDS             13302..14171
                     /locus_tag="OQN12_000182"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1652658.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OQN12_000183"
     CDS             complement(14226..14630)
                     /locus_tag="OQN12_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS1652659.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OQN12_000184"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OQN12_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1652660.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OQN12_000185"
     CDS             15615..17705
                     /locus_tag="OQN12_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1652661.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OQN12_000186"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OQN12_000186"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1652662.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OQN12_000187"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OQN12_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1652663.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OQN12_000188"
     CDS             complement(19673..20275)
                     /locus_tag="OQN12_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1652664.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OQN12_000189"
     CDS             complement(20265..20432)
                     /locus_tag="OQN12_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1652665.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OQN12_000190"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OQN12_000190"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1652666.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OQN12_000191"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OQN12_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1652667.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OQN12_000192"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OQN12_000192"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1652668.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OQN12_000193"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OQN12_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1652669.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OQN12_000194"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OQN12_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1652670.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OQN12_000195"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OQN12_000195"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1652671.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OQN12_000196"
     CDS             28269..28436
                     /locus_tag="OQN12_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS1652672.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OQN12_000197"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OQN12_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OQN12_000198"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OQN12_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1652673.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OQN12_000199"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OQN12_000199"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCS1652674.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OQN12_000200"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OQN12_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS1652675.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OQN12_000201"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OQN12_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1652676.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OQN12_000202"
     CDS             complement(33764..34849)
                     /locus_tag="OQN12_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1652677.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OQN12_000203"
     CDS             complement(34861..36165)
                     /locus_tag="OQN12_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1652678.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OQN12_000204"
     CDS             complement(36177..36530)
                     /locus_tag="OQN12_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS1652679.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OQN12_000205"
     CDS             complement(36541..37419)
                     /locus_tag="OQN12_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1652680.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OQN12_000206"
     CDS             complement(37416..38642)
                     /locus_tag="OQN12_000206"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1652681.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OQN12_000207"
     CDS             complement(38643..39806)
                     /locus_tag="OQN12_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCS1652682.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OQN12_000208"
     CDS             complement(39890..40252)
                     /locus_tag="OQN12_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1652683.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OQN12_000209"
     CDS             complement(40269..41174)
                     /locus_tag="OQN12_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652684.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OQN12_000210"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OQN12_000210"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1652685.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OQN12_000211"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OQN12_000211"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1652686.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OQN12_000212"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OQN12_000212"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1652687.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OQN12_000213"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OQN12_000213"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1652688.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46116)
                     /gene="damX"
                     /locus_tag="OQN12_000214"
     CDS             complement(44830..46116)
                     /gene="damX"
                     /locus_tag="OQN12_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1652689.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46208..47296)
                     /gene="aroB"
                     /locus_tag="OQN12_000215"
     CDS             complement(46208..47296)
                     /gene="aroB"
                     /locus_tag="OQN12_000215"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1652690.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47353..47874)
                     /gene="aroK"
                     /locus_tag="OQN12_000216"
     CDS             complement(47353..47874)
                     /gene="aroK"
                     /locus_tag="OQN12_000216"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1652691.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48275..49513)
                     /gene="hofQ"
                     /locus_tag="OQN12_000217"
     CDS             complement(48275..49513)
                     /gene="hofQ"
                     /locus_tag="OQN12_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1652692.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49425..49829)
                     /gene="hofP"
                     /locus_tag="OQN12_000218"
     CDS             complement(49425..49829)
                     /gene="hofP"
                     /locus_tag="OQN12_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS1652693.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49819..50259)
                     /gene="hofO"
                     /locus_tag="OQN12_000219"
     CDS             complement(49819..50259)
                     /gene="hofO"
                     /locus_tag="OQN12_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1652694.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50243..50782)
                     /gene="hofN"
                     /locus_tag="OQN12_000220"
     CDS             complement(50243..50782)
                     /gene="hofN"
                     /locus_tag="OQN12_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCS1652695.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50782..51561)
                     /gene="hofM"
                     /locus_tag="OQN12_000221"
     CDS             complement(50782..51561)
                     /gene="hofM"
                     /locus_tag="OQN12_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1652696.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51681..54233
                     /gene="mrcA"
                     /locus_tag="OQN12_000222"
     CDS             51681..54233
                     /gene="mrcA"
                     /locus_tag="OQN12_000222"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1652697.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54398..54958)
                     /gene="nudE"
                     /locus_tag="OQN12_000223"
     CDS             complement(54398..54958)
                     /gene="nudE"
                     /locus_tag="OQN12_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1652698.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55278..57413
                     /gene="igaA"
                     /locus_tag="OQN12_000224"
     CDS             55278..57413
                     /gene="igaA"
                     /locus_tag="OQN12_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1652699.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57478..58146
                     /gene="yrfG"
                     /locus_tag="OQN12_000225"
     CDS             57478..58146
                     /gene="yrfG"
                     /locus_tag="OQN12_000225"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1652700.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58157..58558
                     /gene="hslR"
                     /locus_tag="OQN12_000226"
     CDS             58157..58558
                     /gene="hslR"
                     /locus_tag="OQN12_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1652701.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58583..59452
                     /gene="hslO"
                     /locus_tag="OQN12_000227"
     CDS             58583..59452
                     /gene="hslO"
                     /locus_tag="OQN12_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1652702.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59596..61320)
                     /locus_tag="OQN12_000228"
     CDS             complement(59596..61320)
                     /locus_tag="OQN12_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1652703.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61699..63321
                     /gene="pckA"
                     /locus_tag="OQN12_000229"
     CDS             61699..63321
                     /gene="pckA"
                     /locus_tag="OQN12_000229"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1652704.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63397..64749)
                     /gene="envZ"
                     /locus_tag="OQN12_000230"
     CDS             complement(63397..64749)
                     /gene="envZ"
                     /locus_tag="OQN12_000230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1652705.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64746..65465)
                     /gene="ompR"
                     /locus_tag="OQN12_000231"
     CDS             complement(64746..65465)
                     /gene="ompR"
                     /locus_tag="OQN12_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1652706.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65693..66169
                     /gene="greB"
                     /locus_tag="OQN12_000232"
     CDS             65693..66169
                     /gene="greB"
                     /locus_tag="OQN12_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1652707.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66266..68587
                     /locus_tag="OQN12_000233"
     CDS             66266..68587
                     /locus_tag="OQN12_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1652708.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69025..69252
                     /gene="feoA"
                     /locus_tag="OQN12_000234"
     CDS             69025..69252
                     /gene="feoA"
                     /locus_tag="OQN12_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1652709.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69269..71590
                     /gene="feoB"
                     /locus_tag="OQN12_000235"
     CDS             69269..71590
                     /gene="feoB"
                     /locus_tag="OQN12_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1652710.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71590..71826
                     /gene="feoC"
                     /locus_tag="OQN12_000236"
     CDS             71590..71826
                     /gene="feoC"
                     /locus_tag="OQN12_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1652711.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72029..>72896
                     /gene="rpnA"
                     /locus_tag="OQN12_000237"
     CDS             72029..>72896
                     /gene="rpnA"
                     /locus_tag="OQN12_000237"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCS1652712.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAA"
BASE COUNT        17566 a        19751 c        18441 g        17138 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag attgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggtaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattgcacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct ctcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacggatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcccgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 gtcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct agcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acaagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacattacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tgactgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aagaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gtcatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggcgcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact gtacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgcccccc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccttgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaacgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag tacgcttcgg cgtctgtgct accggcttcg gctccgtttt
    45301 cacggttgct tgcggttttt tcggttcaat caccgcctgc tgacgaactg ggcgcgtagt
    45361 ggacggacgt tcagcggttt gcgttttcgc cgagtcacgc gatgcattgc cattgcgaac
    45421 aggcgcaacc gttgcgggtt cagtcggcaa cgtggaattg accgccacat tgttcagctg
    45481 ttgctgattt tgtggctggg tcagcgcatt gttcaggtca ccctgcactt caacacgttg
    45541 ttgaccatcc gttgccaccg gggtttgccc ttgagtcggc gtagaagaga tcggtggcag
    45601 cgaaacatcc tgctgagtat tttccgcaga cgtggttccc ggcgctggct gcacgccatt
    45661 cgcctgatcg gtcgcattgc cagcaagatc aatactcttc tcgccagacg cggtttgatc
    45721 gctggaagtg gtcgaggggg cttttagcgc agaaccgata ccgatgatca acagcagtag
    45781 aaccagaatg ccgacgccca tcatcatata ctgacgagaa gcgggtttac tggctgcttt
    45841 tttgcgctta cgcggacgac gctctacgcg ctcttcatcc acggtttcat cttcggattc
    45901 gtcaatttct tcttcgattt ccggttcctc attgcgctct ttttgcgcac gagtcggacg
    45961 gcgatcgtca gtgtcatcaa gttcaatatc atcaaaattg atctgcggtt cgccacgttc
    46021 agtacgctca gaacgttcag aagattgacg agaacgacca gtacgacgat cgctgggatc
    46081 gggtttcagc tcgtcttctg gtttgaattc atccatttaa caccccacta aaaggttaat
    46141 gcttaccacg ttgcaattaa cctgaagcta atagaccgct tgataagcgg cctgaccttt
    46201 cttgttgtta cgctgattga caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc
    46261 cgctgcgaac ttcactctta ccaattgcca acggaagaat taagcgcatc tctcccgcaa
    46321 ggactttctt gtcacgcagc atatgcggta aatacgcctg cgcggacatt tcgcgcggcc
    46381 cattgaccgg taacccagcc cgcgtgagca gggttataat acgctgcgtt tcggcagaac
    46441 taaactgccc gagacgttcc gacgtccgcg ccgccatcac catacccgca gcgaccgctt
    46501 caccatgtaa ccaattgcca taccccattt cagcttcaat ggcatgacca aaggtgtgtc
    46561 ccagattcag taaagcacgt aacccggttt cgcgctcgtc ggcggcgaca acttctgcct
    46621 tcagttcaca acaacggcga atacagtacg ccattgccgg accgtccaga cgcaacaacg
    46681 catccagatt ctcttccagc cagttaaaaa acgcaccgtc aagaataatg ccgtatttga
    46741 tgacttctgc cagccccgac gctaactcac gcgggggaag cgttttcaga cagtcgagat
    46801 ccaccaccac tgaagcaggc tggtagaacg cgccaatcat gtttttaccg aggggatggt
    46861 tgaccgcagt tttgccgcca acggaggaat cgacctgcga cagtaacgtc gtcgggactt
    46921 gaatgaaacg aacaccgcgc tgataactcg ccgccgcgaa gccggtcaga tcgcccacta
    46981 cgccgccgcc aagcgccacc agcgtagtat cgcgaccatg cggcttttgt aacaacgccg
    47041 taaagacggt atcgagtaca gctaggcttt tatactgctc gccgtcaggg aggataacgc
    47101 tatcgacgtt aacacccgcc tgttcaagta cgccgcggac cttatcgaga tacagaggag
    47161 ccagggtttc gctggtgacc aacatgacct gctcgcccga tttcagcggt aagaatgaag
    47221 ctggttcatt aaacaaacca gatgcgatgg taattgggta actacgttcc ccgagagtaa
    47281 cgacaatcct ctccataacg cgacatccac cttaattact gtacccgcag acgagtgtat
    47341 ataaagccag aattagttgc tttccagcat gtgaataatc tggtttgcaa ccactttagc
    47401 gctttgatca tcagtacgaa tggtcacgtc ggcaatctct tcatacagcg gattgcgttc
    47461 attggccaac gcttccagaa cttcacgcgg cggtgtttca acgtgcagca acgggcgttt
    47521 tttatcacgc tgcgtgcgtg caagttgctt ttcgatggtc gtttcaagat aaacgacaac
    47581 gccacgagcg gaaagacggt tacgcgtttc acgggatttc acagagccgc cgccagtagc
    47641 cagcacaata ccctgtttct cggtcaactc attgatgacc ttttcttcgc gatcgcggaa
    47701 gccttcttcg ccttctaaat cgaaaaccca gcccacatca gctccggttc gtttctcaat
    47761 ctcttgatcg gaatcgtaaa attccatatt gagctgttga gctaactggc gcccaatagt
    47821 gctttttccg gcacccatag gcccaaccag aaagatattg cgtttctctg ccattttttc
    47881 ggtactacta agactattcg ttaatgataa acccgcttcg ctcagagagc gccgcaggac
    47941 atgaactgaa acctcataag atattgcgag agtcagactg aaaattatct caatactcca
    48001 gcgggtttgg caactgaata aatcacccat ccctctgcat atctggtcgc tgcaagcgcg
    48061 ctgccttgct accaccgctc tggcgataaa tcaccgggta agattagcgt aaaaaagaca
    48121 gcaaaatgcc gcctgaatga taaatcatca tcatggaacg caggcagcga agagcaccaa
    48181 ctctcaaatc ggtactcctt gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg
    48241 ttcagcataa aaacattacc gcttttacgg ctgtttactc actggaaacc agtcgtggcg
    48301 tgataaacac cactaactcg cgtcgttcat cttcttttcc gtcatgacga aataattgcc
    48361 cgaaccaggg aatgtcgcca agcaacggta cgctatcctg acccgattta tttttacggg
    48421 taaaaatgcc gcccagcgcc aacgtttctc cgctttttac ctcgacctgc gtttcgatct
    48481 cctgcttatc aatcgccagc acttcgccat cggcctgctg tagcacctgc cccggaacgt
    48541 tctggctgat gtgtaatttc agccggatgc gacctttttg taacaccgtg ggcgtgacct
    48601 ccatccccag gacggcctct ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa
    48661 cctgatatgg aatttcgctc tcctgtttaa tgctggcagg ctgaagatgt gaggccagca
    48721 gacgcggact ggcgataata tccagctgct gtttttgttc gagcgcggaa agctcaagat
    48781 ccagcaagcg tccgttgatg cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta
    48841 cggagaggtc gctaccaagc gtggtgactt gcccaacgcc accagcgtgt tgcgcatcgg
    48901 ccagcgtcca tttcacgcct aactcacgca aacttttttc attaatggtg acaatatgcg
    48961 ccgacagctc aacctgcccg accggcagat ccatttgcgc tacccactgt tcaagcgcgc
    49021 ttaacgccgt tttgttatct cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac
    49081 tccctttggc actcagtagc ttctcccccg ctttcgccag ttctcccgcg tcggcgtatt
    49141 gcagggttat actgcgattt tccagcggca gatttgcctg cgcccgcgcc tgctccgcct
    49201 cctggcgggc gatattgtca ttctgccagg caatggaatg cactgagaga atgttgcctt
    49261 cctgccgcgt tatcagtccg gcgcttttca ctacagtttg tagtgcctgc ttccagggaa
    49321 catctgttag atgtaacgac accgtaccgc tgacgtctgg cgacacgacc aggttcaact
    49381 tctcctgttc agccagcgcc tgcaacacct gagctaccgg aacgtcatcc accatcagcg
    49441 tcactttttg cggctttgcc gcctgtacgc cgggtatcag catcaacagt agtgcggcta
    49501 tccattgctt catttgtatc tccttgccgt tgccacaacc attgtggcgg ttcgcagttt
    49561 gtcccggtac ccagcgttag tacgtctggc gtcagctgta aaattgtcca gccgttttcc
    49621 agcacatcgt tttgctgcac ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg
    49681 atgcgctcgc ctcgccctac catcccctga tagcgccact ggctaagttc gctaatccgg
    49741 catagatctt ccggcgattt aaaagggtca cgcataccgg ttaaaaggca caatgcaata
    49801 cctgccaaca accagcgttt aaccctcatg cggcatctcc agttgtagcg tgaacaaaag
    49861 atcatcacct tccacgctta acgaaaaacg gctcacgctg acgttgcgct ctgccagccg
    49921 tgtaaatgcc gacggcactg cttcccacag cgttttcaac gccaactcgc ctccctgcgc
    49981 ggatggatgc caggaaacca gttgcgcgcc ggataactga aaatccagtg gcgaaaaggg
    50041 cagcgttttt tcctcgctga agggagtggt gtctaccagg cgatacagtg cggcccactg
    50101 ctggtgatga cttgcccgta gtcgaattaa tgcgtcgcgc tcttcatggt gtgtcgatga
    50161 tagaaaaatg agcgtaacta acatcagcaa ccagaatgcc cagcaaaact ggcggaggcg
    50221 gggtgatgtg gcgaaccacc agtcaaagaa catgttcatc gctaaccttc cttgttaact
    50281 gatactcaaa ttgccagcgt ccctgcgcat cctgctgcgt ggctccccgc tgattgagat
    50341 gaaaagacgc atcctggcgg agtgacgttt ctagtgcgtt taacgcggta atgcttgttg
    50401 tcagcccctt gatctccagc gttccctgct gccagcttat cgttgtcagc caggcgtgct
    50461 cgggtaaaag cgccgccagt gcttccagcg cagattgcca gtcgcgggta aattgtcgct
    50521 ggcgctgccg ttgcgaacgc tgttcgcgta actgctgttg ctccagcaaa cgtggcttcg
    50581 ttatctgtaa gctgcgggcg agttgttgtt ccgcctgaag caaaactgcg tctatgcgcg
    50641 cttcggcgct gcctgtcaga cgcagtatta gcgttatccc gacggccagc agcagaggcg
    50701 caacgaacat cagcaaccag aaacgcagaa aagcggtccg gcgttgctgt cgccagggca
    50761 aaaaattaat tggcgggttc atcagtacgc cttcccaagc gccagcccca gcgcgatggc
    50821 aaagtctcca ccaggcggcg gtagcggcgg ctgacgaaca gaaacggcct cccaggggtc
    50881 aaatccgcct tcgccacata tcgcgacgct gtcgggatca acggataacg ctgccgccag
    50941 ctccttcgcg ctagtcatcc ccaccgccag tttgcgcccc cagctatagc gcgtcgccca
    51001 cagccactgt tcgttatcac gccaggccag acattgctga tgagaaggta aaaaaggcag
    51061 gaatcgctgt aatgcactgg catccggggt gatcgcacta acatgaacac gcaacctttc
    51121 tgccagcgta agcagcgttg ccagctcttt gctttgcgcg gcagtcacgt tataagcggg
    51181 gctgagtgag tcttcgctgt aatcgaagcg cagggagtcc ggatccatat ccagctcgcg
    51241 ggccatcgtc cctgacagcc aggccgtttg ctcccgctca ccaagggaca tcgacgggcg
    51301 cggaaatgac cgctgtaatg tgcgactggc gggaaacgcc aacataatgt gatgacgctg
    51361 cggcagttcg cgactccacg gtaacaacgt tttagccagc tgctgcgcat caacaatccg
    51421 cccatctttg ataatgtcgt gcgccagcgg caaccgccac cagcgttgca aaaagcattc
    51481 ttttgcgccc cgcacgatcg caaccgctgc cgcttcttgc tgttgtaaat gcaaaccaat
    51541 ttgccagatc ttaaatgtca ttgtgatgat ctccttatca cccgtcactc tgacgggtat
    51601 atcaatgcgt ctggcttgcc tttatactac cgcgcgtttg tttataaact gcccaaatga
    51661 aactaaatgg gaaatttcca gtgaagttcg taaagtattt tttgatcctt gcagtctgtt
    51721 gcattctgct gggagcaggc tcgatttatg gcctataccg ctacatcgag ccacaactgc
    51781 cggatgtggt gacattaaaa gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg
    51841 atggcgagct gattgctcaa tacggtgaga aacgtcgtat tccggttacg ttggatcaaa
    51901 tcccaccgga gatggtgaaa gcctttatcg cgacggaaga cagccgcttc tacgagcatc
    51961 acggagttga cccggtgggg atcttccgtg cggcaagtgt ggcgctgttc tccggccacg
    52021 cgtcacaagg ggcgagtacc attacccagc agctggcgag aaatttcttc ctcagtccag
    52081 aacgcacgat gatgcgtaag attaaggaag tcttcctcgc gattcgcatt gaacagttgc
    52141 tgacgaaaga cgaaatcctc gagctttatc tgaacaagat ttaccttggt taccgcgcct
    52201 atggtgtcgg tgctgcggca caagtctatt tcggtaaaac ggtcgaccaa ctgacgctga
    52261 acgaaatggc ggtgatagcc gggttgccga aagcgccttc cacctttaat ccgctctact
    52321 caatggatcg tgccgtcgcg cggcgtaacg tcgtactgtc gcggatgctg gatgaagggt
    52381 atatcaccca gcaacagttc gatcagacac gcactgaggc gattaacgct aattatcacg
    52441 cgccggagat tgctttctct gcgccgtacc tgagcgaaat ggtgcgccag gagatgtata
    52501 accgttatgg cgaaagtgcc tatgaagacg gttatcgcat ttacaccacc atcacccgca
    52561 aagtgcagca ggccgcgcag caggcggtac gtaataacgt gctggactac gacatgcgcc
    52621 acggctatcg cggcccggca aatgtgctgt ggaaagtggg cgagtcggcg tgggataaca
    52681 acaagattac cgatacgctg aaggcgctgc cgacctatgg tccgctgctg cctgccgcag
    52741 tcaccagcgc caatcctcag ggagcgacgg cgatgctggc ggacgggtca accgtcgcat
    52801 tgagtatgga aggcgttcgc tgggcgcgtc cttaccgttc ggatactcag cagggaccga
    52861 cgccgcgtaa agtgaccgat gttctgcaaa cgggtcagca aatctgggtt cgtcaggttg
    52921 gcgatgcatg gtggctggca caagtgccgg aagtgaactc ggcgctggtg tcgatcaatc
    52981 cgcaaaatgg tgccgttatg gcgctggtcg gtggctttga tttcaatcag agcaagttta
    53041 accgcgccac ccaggcactg cgtcaggtgg gttccaacat caaaccgttc ctctacaccg
    53101 cggcgatgga taaaggtctg acgctggcaa gtatgttgaa cgatgtgcca atttctcgct
    53161 gggatgcagg tgccggttct gactggcagc cgaagaactc accaccgcag tatgctggtc
    53221 caattcgctt acgtcagggg ctgggtcagt cgaaaaacgt ggtgatggta cgtgcaatgc
    53281 gggcgatggg cgtcgactac gctgcagaat atctgcaacg cttcggcttc ccggcgcaaa
    53341 acattgtcca caccgaatcg ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg
    53401 cgcgcggcta cgcggtcatg gcgaacggcg gcttcctagt ggacccgtgg tttatcagca
    53461 aaattgaaaa cgatcagggc ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg
    53521 aatgcgatat tccagtgatt tacggtgata cgcagaaatc gaacgtgctg gaaaataacg
    53581 atgttgaaga tgtcgctatc tcccgcgagc agcagaatgt ttctgtacca atgccgcagc
    53641 tggagcaggc aaatcaggcg ttagtggcga agactggcgc gcaggagtac gcaccgcacg
    53701 tcatcaacac tccgctggca ttcctgatta agagtgcttt gaacaccaat atctttggtg
    53761 aaccaggctg gcagggtacc ggctggcgtg caggtcgtga tttgcagcgt cgcgatatcg
    53821 gcgggaaaac cggtaccact aacagttcga aagatgcgtg gttctcgggt tacggtccgg
    53881 gcgttgtgac ctcggtctgg attggctttg atgatcaccg tcgtaatctc ggtcatacaa
    53941 cggcttccgg agcgattaaa gatcagatct caggttacga aggcggtgcc aagagtgccc
    54001 agcctgcatg ggacgcttat atgaaagccg ttcttgaagg tgtgccggag cagccgctga
    54061 cgccgcctcc gggtattgtg acggtgaata tcgatcgcag caccgggcag ttagctaatg
    54121 gtggcaacag ccgcgaagag tatttcatcg aaggtacgca gccgacacaa caggcggtgc
    54181 acgaggtggg aacgaccatt atcgataatg gtgaggcaca ggaattgttc tgattaaaaa
    54241 ggcgcttcgg cgccttttca gttggctgac aaagtgcatt tgtttatgcc ggatgcgggt
    54301 gaacgcgtta tccggctaac aaaatcgtga aaaatcaata tattgcagga accccataag
    54361 cctgataagc gttgtgcatc aggcaaactt cacgcattta cactcgcccc tgccctttca
    54421 accattcgcg cacgaggaac agcgcgctga cattacgcgc ttcgttgaag tcagggtctt
    54481 ccagcaaatc catcatatgc gccagcggcc agcgcacctg tggtagcggc tctggctcat
    54541 cgccttccag cgattccggg tagagatctt gcgcgaccac gatattcatt ttgctggaaa
    54601 agtaagacgg tgccatgctg agcttcttca aaaaagtcag atcgttcgca ccaaatccaa
    54661 cctcttcttt tagctcgcgg ttagcggctt cgtagacgct ttcacccgga tcaattaatc
    54721 ctttcgaaaa acctaattcg taggattcag ttcccactgc gtattcgcgg atcaggatca
    54781 ggtgatcatc cacaatcggc acaatcatca ctgcttcccg gttggttgga cgcattcgtt
    54841 cataaacacg ccgcacgcca ttgctgaact ccagatccac gctctcgacg gtaaacagtc
    54901 gggaacgggc tacagtttca acattcagaa tggtgggttt ttgtaatgat ttgttcatcg
    54961 tgggatctat gctgtgaaat cagcggttat tgtgcgatat cggacacgct ttcggcaatg
    55021 tgaattgcat gttatttaca tttatgtaac ttaataaata atcgtcctca aatcaaatta
    55081 aaagtcaata ggttgaaata actccaggaa tttgctgata ttccgccttc agagggtttg
    55141 ctatgatcag cggttactgt gatgtgctta atgatgctca agttaagctc cacgcttgcc
    55201 gatagccaac cgcagaatca tgtattgtgt ccggtgcgac tgaccacgcc tgacagacta
    55261 agtaagatgg ggaaagcatg agcaccattg tgattttttt agctgctttg ctggcctgct
    55321 cactacttgc gggatggctg ataaaagtgc gatccagacg gcgtcagctg ccctggacca
    55381 acgccttcgc ggatgcgcaa acgcgtaaac tcacacctga agaacgtagc gccgttgaaa
    55441 attatcttga gagcctgacg caggtattac aggtgcctgg cccaacggga gctagcgcgg
    55501 caccgatctc tctggcgctg aatgccgaaa gcaacaacgt catgatgctg acacacgcta
    55561 tcacacgtta cggcatctct accgacgatc cgaataaatg gcgttactac ctcgattcgg
    55621 tagaagtcca cctgcccccc ttctgggaac agtacatcaa cgatgagaat accgttgaac
    55681 tgattcatac cgattcgctg ccgttggtta tttcactcaa cggtcatacg ctgcaggagt
    55741 acatgcagga aacgcgcggc tatgccttgc aacctgttcc gtccacgcag gcgtcgattc
    55801 gcggggaaga gagtgagcaa atcgagctac tcaatattcg caaagaaacg catgaagaat
    55861 atgcgctgag tcgtccgcgc gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga
    55921 tgttcttttt ctgcctgatt accccggatg tatttgttcc gtggctggca ggcggcgcat
    55981 tactgctgct gggcgcaggt ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc
    56041 gagaaattca ttgtctgcgc ggtacacccc gtcgttgggg attgtttggc gaaaacgatc
    56101 aggaacagat caacaatatt tcactcggta ttatcgacct ggtctatccc gcacactggc
    56161 agccatacat tgctcaggat ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc
    56221 acgtagtgcg tcaggggcga tatctttcgc tgcatgatga agtaaaaaac tttccgttac
    56281 agcactggct gcgcagtacg attatcgctg cgggctcgct gctggtgctg tttatgctgt
    56341 tattctggat cccgctggat atgccgctga aattcactct ctcatggatg aaaggcgcgc
    56401 agaccattga agccaccagc gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata
    56461 cattgcgtat tagcggtacg ggaatgtgta atattcgcac ttccggaacc tggagcgcga
    56521 aaaccaattc acctttttta ccgtttgact gctcgcagat catctggaat gacgcccgct
    56581 cattaccatt accagaatct gaactggtca acaaagcgac ggcattgact gaagcagtta
    56641 atcgccagct gcaccctaaa ccggaagatg aatctcgcgt cagtgcctca ttgcgttcag
    56701 cgattcaaaa atccggcatg gtattgcttg atgattttgg cgacattgta ctgaagacag
    56761 cggatttatg ttctgccaaa gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg
    56821 gcaacagtaa agactgggac gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg
    56881 tgaatgtgtt attacgcccg gtgagtgcgg aatcgctgga taacctggtg gcaacctcca
    56941 ccgcgccgtt tatcacgcat gaaacggcgc gagcggcaca atcactaaac agtccggccc
    57001 ccggcggatt cctgattgtc agcgacgaag gcagcgattt tgttgatcag ccctggcctt
    57061 cggcatcact ttacgactac ccgccgcaag aacagtggaa cgctttccag aaactggcac
    57121 aaatgctgat gcatacgccg tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg
    57181 ccaatggtac gcagcatatt ggccttcatc cgatcccgga tcgttccggc ctgtggcgct
    57241 atctcagcac cacattgctg ctactgacga tgctgggtag cgccatttac aatggcgtac
    57301 aggcctggcg tcgttaccag cgtcatcgca ctcgcatgat ggagattcag gcctattatg
    57361 aaagctgcct gaacccgcaa ctgatcaccc cttcagaaag ccttatcgaa taacacgttt
    57421 gcgcggcagg ttatgctacc ctgtcgcgca aattgcttca ctctggagat ttccctcatg
    57481 catatcaaca ttgcctggca ggacgtagat accgttctgc tggatatgga cggcacgttg
    57541 ctcgacctcg ccttcgataa ctatttctgg caaaagctgg tgcctgaaac atggggcgcg
    57601 aaaaacgggg ttacgccaca ggaagcgatg gaatatatgc gccagcaata tcacgacgta
    57661 cagcatacgc taaactggta ctgtcttgat tactggagtg agcaactggg tctggatatc
    57721 tgtgcgatga ccaccgagat gggaccgcgc gccgtactgc gtgaagatac cattccgttt
    57781 cttgaggcac tgaaagccag cggtaagcag cgaattttgc tcaccaatgc tcatccgcac
    57841 aacctggcgg taaaacttga gcatacgggt ctggacgcac accttgattt attactttcc
    57901 acccacacat ttggttatcc gaaagaggat cagcggttat ggcatgcggt ggccgaagct
    57961 acgggtctga aagctgaaag aacgctgttt attgatgaca gcgaagcgat tctcgatgct
    58021 gccgcgcaat ttggtattcg ttactgcctc ggcgtgacta atcctgattc cgggattgcc
    58081 gagaaacagt atcaacgcca tccgtcactg aatgactacc gccgcctgat cccctcgcta
    58141 atgtgaagga gacgccatga aagagaaacc tgctgttgag gttcgactgg ataaatggct
    58201 atgggctgcc cgtttttata aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa
    58261 ggtgcattac aacgggcagc gcagcaagcc gagcaaaatc gtcgagctga atgccacgct
    58321 cactctgcgc cagggaaatg acgaacgcac ggtgattgta aaggcgatta ctgaacagcg
    58381 tcgccccgcc agcgaggcag ccttgctgta tgaagagact gcggaaagtg tagagaaacg
    58441 cgaaaaaatg gcgatggcac gtaaacttaa tgccttaacc atgccgcacc cggaccgacg
    58501 cccggacaaa aaagagcgcc gcgacctgtt acgatttaaa cacggcgaca gtgaataact
    58561 gtcacctgca agagagatga ttatgccgca acatgaccaa ttacatcgct atctgtttga
    58621 aaattttgcc gttcgcggcg aactggtaac cgtttcggaa accctgcaac agatccttga
    58681 gaaccacgat tatccgcagc ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag
    58741 cctgttaacc gctacgctga agtttgatgg tgatatcacc gtacagctgc agggcgacgg
    58801 tccgatgaat ctggcggtta ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg
    58861 cgtgcagggc gaaattccag aaaatgccga cctgaaaacg ctggtcggca atggttacgt
    58921 ggtgatcacc attaccccga gcgaaggcga acgctatcag ggcgtagttg gtctggaagg
    58981 tgataccctg gcggcctgcc tagaagatta ctttatgcgt tctgaacagc tgccgacgcg
    59041 cctgtttatt cgcaccggcg acgtagacgg caaaccggct gcaggcggta tgttgttgca
    59101 ggtaatgcct gcgcaaaata cccaacagga cgactttgac cacctggcga cgctaaccga
    59161 aaccatcaaa accgaagaac tgctgacctt accggcaaac gaagtgttgt ggcgtttgta
    59221 tcacgaagaa gaggtgacgg tttacgatcc gcaggatgtg gagttcaaat gcacctgctc
    59281 gcgtgaacgt tgcgccgatg cgctgaaaac gctgcctgat gaagaagttg atagcatcct
    59341 ggcggaagat ggcgaaattg acatgcattg tgattactgc ggtaaccact atctgttcaa
    59401 tgcgatggat attgctgaaa tccgcaacaa cgcgtctccg gcagatccgt aagttcatta
    59461 atgagtatgt ccggcagaga gggctctgcc ggattttacg acttaatggc ctgatgtgct
    59521 gcgcttacca gcgcctatga aataaatcgc aaattatttt ggtcagatac agcgtcgtat
    59581 ccgataaaag cgttgtcact tacttaagtt tatttccagc gtttcaccat ctaccgtcag
    59641 atcgcgccac gctttcggcc tcgtccccag ttttccatcc ttcgcagctg tgagtaattt
    59701 ttctttcgtt atttcgttcg gaagtaaact catatcaagc gcgtcccatt cttttctgtc
    59761 tgagtcaaag ccatagacaa tgactctgtc atcattaaaa gcaaacagga tccgctccgc
    59821 ctgaccatca ctattcagat cttgctcgac cagtacgcag gcgtcttttt caatacaggt
    59881 catcacgtta tagcgatcct ggattaaggc cgaccagaat gtcgcatccg gtttaacaga
    59941 accaggggca attaacacat tttcggcgaa tactttttcc gatacctgtt cctgaagatg
    60001 ttgctctcca tctaacgcca tcagcagatc ccgcgcgcgt ttcgggtctt tcataaaccc
    60061 ggcatcgctt ttcagcgact caagcgccgc acgtccatag cgaccgctct gttcgagcat
    60121 gtagatagtt acctggtctg gcgtgttttt gccgctctga taacgcgcca tatgactgtt
    60181 cacgctaata cgcatactgt ccagcaccgg cgaattaaga agcaccagta tcaccaacac
    60241 caataacgaa accgcaaggt tcactttgcc ctgaagaaca aggggatttt gcccattacg
    60301 ccagacgata ctgacaaaat accccagcga ccacaccagt aacaccagct ccgccagcgc
    60361 gccctgcaag cggtcgacag tccagccgta ctgagcgacc cgcagccata acccccaggt
    60421 ggcgacgaac acatacagcg gagcaaccag caaagcggtt ttaatcaggc aacgcaacgg
    60481 ccccgtccag ggaagtgacg ctttttgcgg atcgcggaca atagccatta agatcaattg
    60541 caaaaaggcc agcgttaaca gcaacccggc ggcggagatg tggcgagaaa tcgcgctcag
    60601 gcccgtaaac ggcagggtga tgataaacat cggggttagc aatgatacta acggcagcaa
    60661 ccccgtggcg attagcgtga acaacttttg aatagagtcg attaaacgtg actgtgtacg
    60721 cgcgaggatc accgccagtg cggtaaccag accaaatgta agatacataa accagtcggt
    60781 tgcaaaaaag agcgtattga aaaacgtgat gccaacaagt ttaaacaact caccccacag
    60841 taaaagcacc agccacgtca ggccattgga gagaaaaatc accagtaata taagtacgtt
    60901 atgccatact gactggtaaa agtagctata gcggctactg ccattgcgga tacgaaggct
    60961 ttgttgtatc cacggtagca acaacattgc catcaacagc agatagcagc caaaatccca
    61021 aatcgccttt tcagctctcc aggggttcat gccatcagtt tgccacttca gccaaccgct
    61081 catccctgtc gtggcaataa acaccagcgc cagccatccc caaaggcgct tctgtttaaa
    61141 agagatcacg gagaagagta aaacggatga aaacgcgacg gtcgcaggca cgccataaac
    61201 aatccaactg tgattttttc cggcaagcca ggcgataagc aggtagcaaa ccagcccctg
    61261 aagtaatccg gtagcaatca tcccccagcg tgttgccggt gaaagttcaa cgttatccat
    61321 gttggttatc cagaatcaaa aggtgggtta attatcgcat ccgggcagta gtattttgct
    61381 tttttcagaa aataatcaaa aaaagttagc gtggtgaatc gatactttac cggttgaatt
    61441 tgcatcaatt tcattcagga atgcgattcc actcacaata ttcccgccat ataaaccaag
    61501 atttaacctt ttgagaacat tttccacacc taaaatgcta tttctgcgat aatagcaacc
    61561 gtttcgtgac aggaatcacg gagttttttg tcaaatatga atttctccag atacgtaaat
    61621 ctatgagcct tgtcgcggtt aacaccccca aaaagacttt actattcagg caatacatat
    61681 tggctaagga gcagtgaaat gcgcgttaac aatggtttgc ccccgcaaga actcgaggct
    61741 tatggtatca gtgacgtaca tgatatcgtt tacaacccaa gctacgacct gctgtatcag
    61801 gaagagctcg atccgagcct gacaggttat gagcgcgggg tgttaactaa tctgggtgcc
    61861 gttgccgtcg ataccgggat cttcaccggt cgttcaccaa aagataagta tatcgtccgt
    61921 gacgatacca ctcgcgatac tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac
    61981 aaacctctct ctccggaaac ctggcagcat ctgaaaggcc tggtgaccag gcagctttcc
    62041 ggcaaacgtc tgttcgttgt cgacgctttc tgtggtgcga acccggatac tcgtctttcc
    62101 gtccgtttca tcaccgaagt ggcctggcag gcgcattttg tcaaaaacat gtttattcgc
    62161 ccgagcgatg aagagctggc aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag
    62221 tgcactaacc cgcagtggaa agaacagggt ctgaactccg aaaacttcgt ggcgtttaac
    62281 ctgaccgagc gcatgcagct gattggcggc acctggtacg gcggcgaaat gaagaaaggg
    62341 atgttctcga tgatgaacta cctgctgccg ctgaaaggta tcgcttctat gcactgctcc
    62401 gccaacgttg gcgagaaagg cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa
    62461 accacccttt ccaccgaccc gaaacgccgc ctgattggcg atgacgaaca cggctgggac
    62521 gatgacggcg tgtttaattt cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa
    62581 gaagcggaac ctgaaatcta caacgctatc cgtcgtgatg cgctgctgga aaacgtcacc
    62641 gtgcgtgaag atggcactat cgactttgat gatggttcaa aaaccgagaa cacccgcgtt
    62701 tcttatccaa tctatcacat cgataacatc gttaagccgg tttccaaagc gggccacgcg
    62761 actaaggtta tcttcctgac tgctgatgct ttcggcgtgt tgccgccggt ttctcgcctg
    62821 actgccgatc aaacccagta tcacttcctc tctggcttca ccgccaaact ggccggtact
    62881 gagcgtggca tcaccgaacc gacgccaacc ttctccgctt gcttcggcgc ggcgttcctg
    62941 tcgctgcacc cgactcagta cgcagaagtg ctggtgaaac gtatgcaggc ggcgggcgcg
    63001 caggcttatc tggttaacac tggctggaac ggcactggca aacgtatctc gattaaagat
    63061 acccgcgcca ttatcgacgc catcctcaac ggttcgctgg ataatgcaga aaccttcact
    63121 ctgccgatgt ttaacctggc gatcccaacc gaactgccgg gcgtagacac gaagattctc
    63181 gatccgcgta acacctacgc ttctccggaa cagtggcagg aaaaagccga aaccctggcg
    63241 aaactgttta tcgacaactt cgataaatac accgacaccc ctgcgggtgc cgcgctggta
    63301 gcggctggtc cgaaactgta atgatttgaa gctggagaat atctatccag tatcttatag
    63361 aaagcaaaac gggaggcacc ttcgcctccc gtttatttac ccttcttttg tcgtgccctg
    63421 cgcccgcgtt accggcactg gcagccaggc gcgaatggaa agcccgcccc gctcgctggt
    63481 gccaagctcc agcatcccgt tatggttatc cacgatacgc tgcacaattg ccagccctaa
    63541 tcccgtgccg ctaatggtgc gcgcactgtc gccgcggaca aacggctgga acaggtgctt
    63601 acgttgttcc ggcgcaattc ccggaccgtc atcttccacc tggaaccagg cgcgattcgg
    63661 ctccgttccg ctgctgactt tgatccagcc attgccataa cgggcggcgt tgaccaccat
    63721 attcgccacc gcgcgtttga tcgacagcgg gtgcattttc acttcaatgc tgccggggta
    63781 aagcgcggtt tcaatttccc gctcatagcc actttcggca gcaatcacct caccgagcac
    63841 tgcattaaga tccgccattt ccatcggcat ctcctgcccg gtgcgcaggt agtcgataaa
    63901 ctgctcaatg atggcgttgc actcttcgat atctttattg atcgattctg ccagatagcc
    63961 atcctgctcg ctcatcatct cagtcgccag gcgaatacgc gtcagcggcg tgcgcaagtc
    64021 gtgacttacc cccgccatca gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc
    64081 catatggtta aaggcacggg taacggaacg cacctccgaa gcgccatatt cacgcagcgg
    64141 cggcggaata atccctttac caacctgcaa ggctgcgtgt tcgagatcga ccaacggtcg
    64201 gttctggata cgaataaaca gccacgcccc gcctatcgcc aatagcataa tcgccagcgt
    64261 atagcggaac agcggagaga aatcgccctg atgaatttcg gtcagcggca cgcgtaccca
    64321 gatattgggc gacagccagg ttttcagcca gacgacaggc gaacttttgt tgacctcaac
    64381 gcgcacttcc gtcgggccgc ccagttgctg tgccatctga tggcttaaga attcatagtg
    64441 ttgcgcccaa cgcagacctg cctcttcggc agcctcgttg gagtagagag agatccccag
    64501 ctcacggtag atctcccgac ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag
    64561 ttgcagtttg tcggtcatca acatacgcac ttcgtacgcg aggactttat taaactgctg
    64621 gaggctcggc aaaatcgcga agttcagcac caccagataa gtcgtcacca ggctggcgaa
    64681 cagcaaggtg acgatgagca ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa
    64741 tcgcctcatg ctttagagcc gtccggtaca aagacgtagc ccaggcccca aacggtctga
    64801 atgtaacgcg gatgcgctgg atcttcttct accatgcggc gcagacgcga aatctgcacg
    64861 tcgatggagc gttccattgc ggaatattca cgaccacggg caaggttcat cagcttatcg
    64921 cgggagagcg gctcacgcgg atggctgacc agtgccttca gtaccgcaaa ctcaccgctg
    64981 gtgagcggca tcggctcatc ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac
    65041 ttaccgaaag caattaccgc ctcttcctgt gacggtgcgc ctggcagttc gttcgcctga
    65101 cgacgcagca ccgcacgaat acgggccagc agttcacgcg ggttaaacgg ttttggaatg
    65161 tagtcgtcag cgccaatctc caggcctacg atacggtcca cttcttcccc tttcgccgtc
    65221 accataatga tcggcatcgg gttgctctga ctacgaagac gtcggcaaat cgacaagcca
    65281 tcttcaccag gtaacattaa atccagtacc ataagatgga aagattcacg agtcagcagg
    65341 cgatccatct gttctgcatt agcgacgctt cgaacctgga agccttgttc ggtgagataa
    65401 cgttccagca gcgcacgcag gcgcatgtcg tcatcgacca ccagaatctt gtagttctct
    65461 tgcattgttt gtactcccaa aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc
    65521 tcacgttcgt caccagctaa atctggtata aatttcagcc taaattgtta caaagcatat
    65581 taaacagcag cttaagtata caatttattc ggcgaaacat tattgattct gttgatatga
    65641 tcacgttata cccaatgtgc gcattatcaa acagacaaag ggaatcaacg agatgaaaac
    65701 gcccctggtt acccgggaag ggtatgaaaa actcaaacaa gagcttaatt atctctggcg
    65761 tgaagaacgc ccggaggtca caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag
    65821 cgaaaatgct gactatcagt ataataaaaa gcgtctgcgt gaaatcgacc gtcgcgtgcg
    65881 ctatctcact aaatgcctgg aaaatctcaa aatcgtcgat tactcccctc agcaggaagg
    65941 caaagtattt tttggcgcgt gggtggagat tgaaaacgac gatggcgtga ctcaccgttt
    66001 ccgtattgtc ggctacgatg aaatttttgg ccgtaaagat tacatctcta tcgattcccc
    66061 gatggcccgc gcattgctga aaaaagaagt cggcgatctg gcggtggtga atacccctgc
    66121 cggggaagcg aactggtatg ttaatgctat cgagtacgtg aaaccgtaag gaagagtctt
    66181 aacctcctgc cgatggctgg catttttgcc agccagtccg tataactatc ccctgatttt
    66241 tgatccgaaa agatgaactc aaaccatgat gaatgattcg ttctgccgca ttattgcggg
    66301 tgaaattcag gcgcgcccgg aacaggttga cgctgccgtt cgcctgcttg acgaagggaa
    66361 taccgtgccg tttatcgcac gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca
    66421 gctgcgtaat ctggaaacgc gtctgagcta tctgcgcgag ctggaagaga gacgtcaggc
    66481 gatcctcaaa tccatttccg agcaaggcaa actcaccgat gatctggcga aggccatcaa
    66541 cgccacccta agcaaaaccg aactcgaaga cctctacctg ccctacaaac ctaaacgccg
    66601 cacccgcggg caaatcgcca ttgaagcagg gcttgagccg ttggctgacc tgctgtggag
    66661 cgatccgtca cacacgccag aagtcgccgc tgcacaatat gttgatgccg ataaaggcgt
    66721 ggcagatacc aaagccgcgc tggatggcgc gcgctatatc ctgatggaac ggtttgccga
    66781 agatgccgcg ctgctggcga aagtgcgtga ttatctgtgg aagaacgcgc atttggtttc
    66841 tacggtggtg agcggtaaag aagaggaagg ggcgaaattc cgcgactatt tcgatcatca
    66901 cgaaccgttg tccacggtgc cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga
    66961 aggcgtactc cagctttcgc tgaatgccga tccgcagttc gatgagccgc ccaaagagag
    67021 ctattgcgag caaatcatca tggatcacct tggcctgcgc ctgaacaatg ccccggcgga
    67081 tagctggcgc aaaagcgtag tgagctggac ctggcgcatc aaggtgctga tgcatctgga
    67141 aaccgaactg atgggtaccg tgcgcgaacg cgcggaagat gaagcaatca acgtctttgc
    67201 ccgtaacctg cacgatctgc tgatggcggc ccctgccgga ctgcgtgcaa cgatgggcct
    67261 cgatccgggt ctgcgtaccg gggtaaaagt ggcggtggtc gatgccactg gcaaactggt
    67321 ggcgaccgat accatttacc cgcacaccgg acaagccgca aaagcagcga tgaccgttgc
    67381 tgccttgtgt gaaaaacata acgttgaact ggtggcgatc ggcaacggta cagcttcccg
    67441 cgaaactgaa cgtttctatc tcgacgtgca gaagcagttc ccgaaagtga ccgcacagaa
    67501 agtgatcgtc agcgaagctg gcgcgtcggt ttactcagct tccgagctgg cagcgcagga
    67561 gttcccggat ctcgacgttt cgctgcgtgg tgcggtatct atcgcccgcc gtttgcagga
    67621 tccgctggcg gagctggtga aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca
    67681 tgacgtcagc cagacgcaac tggcccgcaa actggatgca gtagtagaag actgcgtaaa
    67741 cgccgttggc gtcgatctca acaccgcttc tgttccgcta ttaacccgcg tggcgggcct
    67801 gacgcgcatg atggcgcaaa acatcgtggc ctggcgcgat gagaacggcc agttccagaa
    67861 ccgtcagcaa ctgctgaaag tcagccgtct ggggccgaaa gccttcgagc agtgcgcggg
    67921 cttcctgcgc attaaccacg gtgataaccc gctggacgcc tctaccgttc atccggaagc
    67981 ctatccggtg gtggaacgca ttctggcagc aacacagcag gcactgaaag atctgatggg
    68041 taacagcagc gaactgcgta acctgaaagc gtctgacttt actgatgaaa aattcggtgt
    68101 gccgacagta actgacatca tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga
    68161 atttaaaacc gctcagtttg ccgatggcgt cgagacaatg aacgacctgc aaccgggtat
    68221 gatcctcgaa ggcgcagtga ccaacgtcac caactttggc gcgtttgtcg atattggcgt
    68281 gcatcaggac ggcctggttc acatctcttc attatcgaac aagtttgtgg aagatccgca
    68341 taccgtggtg aaagcgggcg acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg
    68401 taaacgtatc gccctgacta tgcgtctgga cgagcagcct ggcgaaacca actctcgtcg
    68461 cggcggcggt aatgaacgcc cgcaaaacaa ccgcccggca gccaaaccac gcggtcgtga
    68521 agcgcagcct gccggtaata gcgcgatgat ggatgcgctg gcggcagcga tgggtaaaaa
    68581 acgttaaacg cccggactgg cccctaaacc tgcgcgataa ggcgttcacg ccgcatctgg
    68641 caacgaacgc tttgcctgac ataaaagtgc cggagaatat ctccggcact tttattccac
    68701 agccaaactc acaatatact ccgttaatat ttatcacttc attaacaact gaaaccttaa
    68761 ttaaacatta gccagtccgg gtaactcact attcgaatta tattttcgct gcgatataac
    68821 cttgagccac atcaacattg agtcagatta ttattcaaac caacattcgc acacatttta
    68881 agtattgctg atagaaacca ttctcattat cattgtgttg ttgattattt aatctctcct
    68941 ttgttggcaa atcatctggt ctcatgtcgc ggtcaaacgc cccatgaggt agttatccag
    69001 ttaatgagaa acaagtaggc acctatgcaa tacactccag atactgcgtg gaaaatcact
    69061 ggcttttccc gtgaaatcag cccggcatat cgccaaaaac tgctttctct aggcatgtta
    69121 cctggctcct cttttgatgt ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa
    69181 acccgtcgtg tgagcctggt attacgcaaa aaagatctgg ccttattaga agtggaagcg
    69241 gtttcctgtt aatacggtga taacaacaat gaaaaaatta accattggct taattggtaa
    69301 tccaaattct ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg
    69361 taactgggct ggcgttaccg tcgaacgtaa agaagggcaa ttctccacca ccgatcatca
    69421 ggtcacgctg gtggacctgc ccggcaccta ttctctgacc accatctcat cgcagacctc
    69481 gctcgatgag caaatcgcct gtcactacat tttgagtggc gacgccgacc tgctgattaa
    69541 cgtggtggat gcgtctaacc ttgagcgtaa cctgtacctg acgctgcaac tgctggaact
    69601 cggcattccc tgcatcgtgg cgctgaacat gctcgacatt gccgagaagc aaaatattcg
    69661 tattgaaatt gatgctctat cggcacgtct tggctgcccg gtgatcccgc tggtttcaac
    69721 ccgtggtcgc ggtattgaag cgctcaagct ggcgattgat cgctataaag ctaacgagaa
    69781 tgtggaactg gtgcattacg cacagccgct actcaacgaa gcagattcac tggcaaaagt
    69841 gatgccttcc gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg
    69901 cgatatctac agccgcgcct acgccggtga agcgtcgcag catctggatg ccaccctcgc
    69961 ccgtctgcgt aatgaaatgg atgatccggc gctgcacatt gccgatgcgc gttaccagtg
    70021 cattgctgcc atctgtgatg tggtaagcaa caccctgacg gcagaaccca gccgtttcac
    70081 cactgcggta gataaaatcg tgctcaaccg tttcctcggt ctgccgattt tcctctttgt
    70141 gatgtacctg atgttcctgc tggctatcaa catcggcggg gcgttacagc cgctgtttaa
    70201 cgtcggctcc gtggcgctat ttgtgcatgg tattcaatgg attggctaca cgctccactt
    70261 cccggactgg ctgactatct tcctcgccca gggcctgggt ggcggtatta acaccgtgct
    70321 gccactggtg ccgcagatcg gcatgatgta cctgttcctc tccttccttg aggactccgg
    70381 ctatatggcg cgtgcagcgt ttgtgatgga ccgcctgatg caggcgctgg gcttgccggg
    70441 gaaatccttt gtgccgctga tcgtcggttt cggttgtaac gtaccgtcgg taatgggcgc
    70501 acgtacgctt gatgcaccgc gtgaacgtct gatgaccatc atgatggcac cgtttatgtc
    70561 ctgcggcgcg cgtctggcta tcttcgcagt attcgcggct gccttcttcg ggcagaacgg
    70621 tgccctggcg gttttctcgc tgtatatgct cggtatcgtg atggcggtgc tgactggcct
    70681 gatgctcaag tacaccatca tgcgcggtga agcgacgccg tttgtcatgg agctgccggt
    70741 ctaccatgta ccacacgtta aaagcctgat tatccagacc tggcagcgtc tgaagggctt
    70801 cgttctgcgt gctggtaaag tgattatcat cgtcagcatt ttcctgagcg ctttcaacag
    70861 cttctcgttg agcgggaaaa tcgtcgataa catcaacgac tcggcgctgg cttccgtcag
    70921 ccgggtgata acccctgtct tcaagccgat tggcgtacat gaagataact ggcaggcaac
    70981 ggttggcctg tttacgggcg caatggcaaa agaagtggtg gtgggtacgc tcaacaccct
    71041 ctacaccgca gaaaatattc aggacgaaga gttcaatccg gcggaattca acctcggtga
    71101 agagctgttc agtgcggtag atgaaacctg gcagagcctg aaagacacct tcagccttag
    71161 cgtactgatg aaccccattg aagctagcaa aggcaacggt gaaatgggta ccggggcgat
    71221 gggcgtgatg gatcagaaat tcggtagcgc agctgccgca tacagctacc tgattttcgt
    71281 cctgctgtat gtaccatgta tctcggtgat gggagccatc gcccgtgaat caagccgtgg
    71341 ctggatgggc ttctccatcc tgtgggggct gaatatcgct tactcactgg caacattgtt
    71401 ctatcaggtc gccagctaca gtcagcaccc aacttacagc ctggtgtgca ttctggcggt
    71461 aatcctgttt aacatcgtag ctatcggtct gctgcgccgc gcgcgtagcc gggtggatat
    71521 cgaactgctg gcaacccgca agtcggtaag cagttgctgc gcagccagca ccaccggtga
    71581 ttgccattaa tggcttcact tattcaggta cgtgatttgc tggcgttacg gggccgtatg
    71641 gaagcgaccc agataagcca gacattgaac actccgcagc caatgattaa cgccatgctg
    71701 caacagctgg aaagcatggg caaagccgtg cggattcagg aagaacctga cggctgcctc
    71761 tctggcagtt gtaaaagctg cccggaaggc aaagcctgtc tgcgcgagtg gtgggcgctg
    71821 cgttaaccat actccatcgc ctgttttgaa aagcccggta tgcgtttgca tccgggcttt
    71881 tttgcgtgcg gctttccata aaaatgcaat tcttgcagcg caacgtaagt tcctctgaaa
    71941 gcgtctcgca gggatgaaaa ctcgctaatg cacagatgtg gagtggcgcg tagagtcgcg
    72001 gcattcaaac aacaggtgaa ggaacgccat gagtaaaaag cagagttcca ccccacatga
    72061 tgcgctgttc aaactctttt tacgccaacc ggagacggca cgcgattttc ttgcgtttca
    72121 tttaccggca cccattcacg cgctctgtga tatgaaaacc ctcaagctgg agtcgaggag
    72181 ctttattgat gacgatctgc gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga
    72241 acaaggacca ggatacatct attgtctgat tgaacatcaa agcacctcaa acaaactgat
    72301 cgcatttcgc atgatgcgtt acgctattgc cgcaatgcaa aatcaccttg atgctggata
    72361 caaaacgttg ccgatggtgg tgccattgtt gttttaccac ggtattgaaa gcccctatcc
    72421 ctattcgctg tgttggctgg attgtttcgc cgatcccaaa ctggcaaggc agctttatgc
    72481 ctccgcattt ccgctgattg atgtcaccgt catgcctgat gatgaaatca tgcagcaccg
    72541 acgtatggcg ctgctggagt taattcaaaa acatattcgt caacgcgatc tgatggggct
    72601 ggtagagcaa atggcctgct tattaagtag tggatacgct aatgacagac aaatcaaagg
    72661 gctgtttaat tacatactgc aaactggcga cgctgtacgt tttaacgatt ttatcgacgg
    72721 cgttgccgaa cgttcaccga aacacaagga gagtttaatg actattgcgg aaagattgcg
    72781 gcaggagggg gaacaatcca aagccctgca tatagccaaa ataatgcttg aatccggagt
    72841 ccctcttgca gacatcatgc gatttaccgg gctgtcagaa gaagagttgg ctgcgg
//
LOCUS       DAJVRP010000004        71518 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVRP010000004 DAJVRP010000000
VERSION     DAJVRP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.004"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(750..2345)
                     /locus_tag="OQN12_000238"
     CDS             complement(750..2345)
                     /locus_tag="OQN12_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCS1652713.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2700..4871)
                     /gene="glcB"
                     /locus_tag="OQN12_000239"
     CDS             complement(2700..4871)
                     /gene="glcB"
                     /locus_tag="OQN12_000239"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCS1652714.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4893..5297)
                     /locus_tag="OQN12_000240"
     CDS             complement(4893..5297)
                     /locus_tag="OQN12_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCS1652715.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5302..6521)
                     /gene="glcF"
                     /locus_tag="OQN12_000241"
                     /pseudo
     CDS             complement(5302..6521)
                     /gene="glcF"
                     /locus_tag="OQN12_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6532..7584)
                     /gene="glcE"
                     /locus_tag="OQN12_000242"
     CDS             complement(6532..7584)
                     /gene="glcE"
                     /locus_tag="OQN12_000242"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCS1652716.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7584..9083)
                     /gene="glcD"
                     /locus_tag="OQN12_000243"
                     /pseudo
     CDS             complement(7584..9083)
                     /gene="glcD"
                     /locus_tag="OQN12_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9334..10125
                     /gene="glcC"
                     /locus_tag="OQN12_000244"
     CDS             9334..10125
                     /gene="glcC"
                     /locus_tag="OQN12_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCS1652717.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARSAQRAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10249..11390)
                     /locus_tag="OQN12_000245"
                     /pseudo
     CDS             complement(10249..11390)
                     /locus_tag="OQN12_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11752..13482
                     /locus_tag="OQN12_000246"
     CDS             11752..13482
                     /locus_tag="OQN12_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCS1652718.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13479..14393
                     /locus_tag="OQN12_000247"
     CDS             13479..14393
                     /locus_tag="OQN12_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCS1652719.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14426..14674
                     /locus_tag="OQN12_000248"
     CDS             14426..14674
                     /locus_tag="OQN12_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1652720.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14674..15845
                     /locus_tag="OQN12_000249"
                     /pseudo
     CDS             14674..15845
                     /locus_tag="OQN12_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15881..16960)
                     /gene="lptG"
                     /locus_tag="OQN12_000250"
     CDS             complement(15881..16960)
                     /gene="lptG"
                     /locus_tag="OQN12_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1652721.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16957..18027)
                     /gene="lptF"
                     /locus_tag="OQN12_000251"
     CDS             complement(16957..18027)
                     /gene="lptF"
                     /locus_tag="OQN12_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1652722.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18058..18618)
                     /locus_tag="OQN12_000252"
     CDS             complement(18058..18618)
                     /locus_tag="OQN12_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1652723.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18630..19454)
                     /locus_tag="OQN12_000253"
     CDS             complement(18630..19454)
                     /locus_tag="OQN12_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652724.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19454..20803)
                     /locus_tag="OQN12_000254"
     CDS             complement(19454..20803)
                     /locus_tag="OQN12_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS1652725.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20849..21607)
                     /locus_tag="OQN12_000255"
     CDS             complement(20849..21607)
                     /locus_tag="OQN12_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652726.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21639..22352)
                     /locus_tag="OQN12_000256"
     CDS             complement(21639..22352)
                     /locus_tag="OQN12_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1652727.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22526..23218
                     /locus_tag="OQN12_000257"
     CDS             22526..23218
                     /locus_tag="OQN12_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1652728.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23267..24766)
                     /gene="pitB"
                     /locus_tag="OQN12_000258"
     CDS             complement(23267..24766)
                     /gene="pitB"
                     /locus_tag="OQN12_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCS1652729.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25058..26917)
                     /gene="gss"
                     /locus_tag="OQN12_000259"
     CDS             complement(25058..26917)
                     /gene="gss"
                     /locus_tag="OQN12_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1652730.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27122..27988
                     /gene="yghU"
                     /locus_tag="OQN12_000260"
     CDS             27122..27988
                     /gene="yghU"
                     /locus_tag="OQN12_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1652731.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28111..28359)
                     /gene="hybG"
                     /locus_tag="OQN12_000261"
     CDS             complement(28111..28359)
                     /gene="hybG"
                     /locus_tag="OQN12_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1652732.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28493..28834)
                     /gene="hypA"
                     /locus_tag="OQN12_000262"
     CDS             complement(28493..28834)
                     /gene="hypA"
                     /locus_tag="OQN12_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1652733.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28827..29315)
                     /gene="hybE"
                     /locus_tag="OQN12_000263"
     CDS             complement(28827..29315)
                     /gene="hybE"
                     /locus_tag="OQN12_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1652734.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(29308..29802)
                     /locus_tag="OQN12_000264"
     CDS             complement(29308..29802)
                     /locus_tag="OQN12_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1652735.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29802..31505)
                     /gene="hybC"
                     /locus_tag="OQN12_000265"
     CDS             complement(29802..31505)
                     /gene="hybC"
                     /locus_tag="OQN12_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1652736.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31502..32680)
                     /gene="hybB"
                     /locus_tag="OQN12_000266"
     CDS             complement(31502..32680)
                     /gene="hybB"
                     /locus_tag="OQN12_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1652737.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32670..33656)
                     /gene="hybA"
                     /locus_tag="OQN12_000267"
     CDS             complement(32670..33656)
                     /gene="hybA"
                     /locus_tag="OQN12_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1652738.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33659..34777)
                     /gene="hybO"
                     /locus_tag="OQN12_000268"
     CDS             complement(33659..34777)
                     /gene="hybO"
                     /locus_tag="OQN12_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1652739.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34966..35253)
                     /locus_tag="OQN12_000269"
     CDS             complement(34966..35253)
                     /locus_tag="OQN12_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1652740.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(35372..36259)
                     /locus_tag="OQN12_000270"
     CDS             complement(35372..36259)
                     /locus_tag="OQN12_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1652741.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36416..37456
                     /gene="gpr"
                     /locus_tag="OQN12_000271"
     CDS             36416..37456
                     /gene="gpr"
                     /locus_tag="OQN12_000271"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1652742.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37496..37990)
                     /locus_tag="OQN12_000272"
     CDS             complement(37496..37990)
                     /locus_tag="OQN12_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1652743.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38181..39065
                     /gene="yghA"
                     /locus_tag="OQN12_000273"
     CDS             38181..39065
                     /gene="yghA"
                     /locus_tag="OQN12_000273"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1652744.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39337..39762)
                     /gene="exbD"
                     /locus_tag="OQN12_000274"
     CDS             complement(39337..39762)
                     /gene="exbD"
                     /locus_tag="OQN12_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1652745.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39769..40503)
                     /gene="exbB"
                     /locus_tag="OQN12_000275"
     CDS             complement(39769..40503)
                     /gene="exbB"
                     /locus_tag="OQN12_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1652746.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40755..41942
                     /gene="metC"
                     /locus_tag="OQN12_000276"
     CDS             40755..41942
                     /gene="metC"
                     /locus_tag="OQN12_000276"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1652747.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42082..42741
                     /locus_tag="OQN12_000277"
     CDS             42082..42741
                     /locus_tag="OQN12_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1652748.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42781..43680)
                     /gene="yqhC"
                     /locus_tag="OQN12_000278"
     CDS             complement(42781..43680)
                     /gene="yqhC"
                     /locus_tag="OQN12_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1652749.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43874..45037
                     /gene="yqhD"
                     /locus_tag="OQN12_000279"
     CDS             43874..45037
                     /gene="yqhD"
                     /locus_tag="OQN12_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1652750.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45142..45969
                     /gene="dkgA"
                     /locus_tag="OQN12_000280"
     CDS             45142..45969
                     /gene="dkgA"
                     /locus_tag="OQN12_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1652751.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46169..47095
                     /locus_tag="OQN12_000281"
     CDS             46169..47095
                     /locus_tag="OQN12_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1652752.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47146..47403
                     /gene="yqhH"
                     /locus_tag="OQN12_000282"
     CDS             47146..47403
                     /gene="yqhH"
                     /locus_tag="OQN12_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1652753.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47446..49665)
                     /locus_tag="OQN12_000283"
     CDS             complement(47446..49665)
                     /locus_tag="OQN12_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS1652754.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49776..51188)
                     /gene="ftsP"
                     /locus_tag="OQN12_000284"
     CDS             complement(49776..51188)
                     /gene="ftsP"
                     /locus_tag="OQN12_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1652755.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51263..52000)
                     /gene="plsC"
                     /locus_tag="OQN12_000285"
                     /gene_synonym="parF"
     CDS             complement(51263..52000)
                     /gene="plsC"
                     /locus_tag="OQN12_000285"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1652756.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52234..54492)
                     /gene="parC"
                     /locus_tag="OQN12_000286"
     CDS             complement(52234..54492)
                     /gene="parC"
                     /locus_tag="OQN12_000286"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1652757.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55038..55520)
                     /locus_tag="OQN12_000287"
     CDS             complement(55038..55520)
                     /locus_tag="OQN12_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCS1652758.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55573..55965)
                     /gene="ygiW"
                     /locus_tag="OQN12_000288"
     CDS             complement(55573..55965)
                     /gene="ygiW"
                     /locus_tag="OQN12_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1652759.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56117..56776
                     /gene="qseB"
                     /locus_tag="OQN12_000289"
     CDS             56117..56776
                     /gene="qseB"
                     /locus_tag="OQN12_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1652760.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56773..58047
                     /gene="qseC"
                     /locus_tag="OQN12_000290"
     CDS             56773..58047
                     /gene="qseC"
                     /locus_tag="OQN12_000290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1652761.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58093..58425)
                     /locus_tag="OQN12_000291"
     CDS             complement(58093..58425)
                     /locus_tag="OQN12_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCS1652762.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58744..59325
                     /gene="mdaB"
                     /locus_tag="OQN12_000292"
     CDS             58744..59325
                     /gene="mdaB"
                     /locus_tag="OQN12_000292"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1652763.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59356..59670
                     /locus_tag="OQN12_000293"
     CDS             59356..59670
                     /locus_tag="OQN12_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1652764.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59718..61610)
                     /gene="parE"
                     /locus_tag="OQN12_000294"
     CDS             complement(59718..61610)
                     /gene="parE"
                     /locus_tag="OQN12_000294"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1652765.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61639..62220)
                     /gene="yqiA"
                     /locus_tag="OQN12_000295"
     CDS             complement(61639..62220)
                     /gene="yqiA"
                     /locus_tag="OQN12_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1652766.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62220..63047)
                     /gene="cpdA"
                     /locus_tag="OQN12_000296"
     CDS             complement(62220..63047)
                     /gene="cpdA"
                     /locus_tag="OQN12_000296"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1652767.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63072..63494)
                     /locus_tag="OQN12_000297"
     CDS             complement(63072..63494)
                     /locus_tag="OQN12_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS1652768.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63495..64124)
                     /gene="nudF"
                     /locus_tag="OQN12_000298"
     CDS             complement(63495..64124)
                     /gene="nudF"
                     /locus_tag="OQN12_000298"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1652769.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64329..65810
                     /gene="tolC"
                     /locus_tag="OQN12_000299"
     CDS             64329..65810
                     /gene="tolC"
                     /locus_tag="OQN12_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1652770.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65958..66629
                     /locus_tag="OQN12_000300"
     CDS             65958..66629
                     /locus_tag="OQN12_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1652771.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66635..67795
                     /locus_tag="OQN12_000301"
     CDS             66635..67795
                     /locus_tag="OQN12_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1652772.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67833..68621)
                     /gene="ygiD"
                     /locus_tag="OQN12_000302"
     CDS             complement(67833..68621)
                     /gene="ygiD"
                     /locus_tag="OQN12_000302"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1652773.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68764..69537
                     /gene="zupT"
                     /locus_tag="OQN12_000303"
     CDS             68764..69537
                     /gene="zupT"
                     /locus_tag="OQN12_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1652774.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69595..69765)
                     /locus_tag="OQN12_000304"
     CDS             complement(69595..69765)
                     /locus_tag="OQN12_000304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652775.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70026..70679)
                     /gene="ribB"
                     /locus_tag="OQN12_000305"
     CDS             complement(70026..70679)
                     /gene="ribB"
                     /locus_tag="OQN12_000305"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1652776.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70783..70931)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71053..71343
                     /gene="ubiK"
                     /locus_tag="OQN12_000306"
     CDS             71053..71343
                     /gene="ubiK"
                     /locus_tag="OQN12_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS1652777.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17840 a        19067 c        17670 g        16941 t
ORIGIN      
        1 tcatttattc acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca
       61 caaacaggca ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca
      121 taatcaccaa attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg
      181 ttggagttaa aatacataca taatattaac atctgccaat atttcacttt ccgcaagacg
      241 gatgatttcg aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta
      301 acgaacactg ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt
      361 ggagataaat tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa
      421 aatcagcttt gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt
      481 gttctagtga aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga
      541 agcaatgtaa taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca
      601 gaagccaagt tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag
      661 aagccaatcg taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt
      721 gatatttacc ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac
      781 gcctgcagca gggtgataac gccgataacg ctggcaaaaa tcagactgtg cttcacggtg
      841 tagcggaaca gttcagattc tcggcccacc atgcccgtcg cggcgcaggc cacggcgata
      901 gattgcgggg agatcatctt gccagttacg ccgccgctgg tatttgctgc caccagcaga
      961 gtgtcagaga cgttgatttg ctgcgccgtg gtcgattgca gtgaaccaaa cagggcgttt
     1021 gaagaagtgt ccgacccggt aaggaatacg cccagccagc cgagaaacgg tgagaaaaac
     1081 gggaacatca cgcctgtacc tgccagtacc agcgccagcg tggtggacat gccggaatag
     1141 ttggtgacga aggcgaatgc cagcaccatg ccaatcgaca atatcggcca cttcagacta
     1201 attagcattt cggcaaagac gccaatccct ttcttgatgc ccacgccgag gatgaagata
     1261 gagataatcg ccgcaataaa aatagcggtg ccgccagccg agagagggtc gaatttaaac
     1321 accgcatcca ttggcgttgg ttgggcgaca atgggtgccg ctttcaacac ttgttgatgc
     1381 aaatgaggga tctggaaatt aatcaccagt gaataaaacg cgccgcccgg agcaaataac
     1441 gctttaaacg gcttcatggt ccagatggtg accagcaccg ttaagattaa aaacggtgac
     1501 cacgcacgaa tgatttgccc cagactatat tctgaaggca cgggaccgcc agaagatggc
     1561 ttatttacca ccatcgcacc tgcggattgc cccatgctga ttgccgtttc ggtatttttc
     1621 ggccgccaga ctttaagaaa taaagcgagt gagacgatac tcaccagcgc cgaagtaata
     1681 tccggcagtt ccggaccaat atagttagag gtaaagaact gagtgacagc gaagcttccc
     1741 ccagcaacca gcgccgctgg ccacgtctct ttcacccctt tccagccgtc catcattgct
     1801 accagccaga acggcacaag aaccgacagg aacggtaact gacgtcccgc cattgcgcca
     1861 atgtggaacg gatcgattcc cgttacctga ccggcgacca gaatcggcac gcccaacgca
     1921 ccaaacgcca ccgtcgcacc aaagccagca gctccttcca gcaacgcacc aaaggagaaa
     1981 ccaatcagta acacctgcaa acgctgatcg tcggtgatgg agataaccaa gctgcggata
     2041 atatcgaact gcccgctggc aacggttaat ttatacagga acaccgccgc gacaataatc
     2101 cacgcaattg gccataatcc ataaataaag ccatagcccg cagcagcaaa tgccatatca
     2161 atcggcattt taaaggcgaa tatcgcaatc aggatagata atataagggt tattgctcca
     2221 gcgacatgtc ctttcagacg taataccgcg agtgcaacga agaagaatat tatcgggatc
     2281 agggcgacca gagcggatag ccccagtcct cccatcggca tatacatttg ggtccaggta
     2341 accatattgt tcagtctctt attatcttta tatgcttgat atacttaagg ttgtaataag
     2401 caaaagagga ctgaactgta aaatataggc gttatacttt acagcaacag tacgccgcta
     2461 acgcaattgc tacctctggc ataacaagta tatcgggtaa gggtttctgt tccgcacacg
     2521 caggcgcaga gtatcgttaa gatgtccata ttgttgtttt aggcccgcta gtaatgcgct
     2581 acgggtattt aatattgtta aaccctgata atcgctccgg ttatttccgg gataaatgta
     2641 ctaccgcagt tactatcata gccccgacaa taaaacttgc cggggctttt ttgacgctat
     2701 taatgacttt ctttttcgcg taaacgccag gcgtgtaata acggttcggt ataaccgttt
     2761 ggctgtttca cgccgaggaa gattaaatcg ctggcagctt taaaagcaca cgagttagcg
     2821 aaattccccg ccatcggacg ataagccgga tcgccagcat tttgctgatc aaccactttc
     2881 gccatattct ccagcgacgc ctgcacctgt tctttggtca gaataccgtg acgtaaccag
     2941 ttggcgatat gctggctgga gatacgcagc gttgcgcggt cttccatcag cgccacgttg
     3001 tgaatatccg gcacttttga acaaccaatc ccctgctcca cccaacgcac cacgtacccc
     3061 agaatcccct gcacgttgtt atccagctct tgttggatct cttgcgccga ccagttagcg
     3121 ttttcagcca ccggaatagt cagcagatcg tccagcagcg gttcaaattc agcactgaac
     3181 tcggtctggg caatgttggc ttgcacgctc tgtacgttgg tatggtggta gtgcagcgca
     3241 tggagcgtag cagcggttgg tgacggaacc caggctgtgt ttgccccggc acgcagttgg
     3301 tcgcccttct ggctgtacat gtctgccatc aggtccggca ttgcccacat gcctttaccg
     3361 atttgcgctt taccgcgcag cccacagaac agaccggaaa gcacgttatt acgttcgtag
     3421 gctttgatcc acggcgtcga tttcatctgg tttttacgca gcatcgggcc agcttccatc
     3481 accgaatgca tttcatcgcc ggtacggtcg aggaaaccgg tattgatgaa cgccacgcgg
     3541 ttgcgcgcct gagcgataca gctacgcaag ttcagcgagg tccggcgttc ttcatccata
     3601 atgcccattt tcagggtatt cggtgccata ccgagcattg tctcaatgcg ggtaaacagt
     3661 ttgttggcga acgccacttc ctgcggaccg tgcattttcg gtttcacaat atagacgctg
     3721 ccagtgcgcg agtttttctg cacttttaaa tcatagaggg caatcgcgcc agtcatgacg
     3781 ccatcaagaa taccttccgg gatttcattg ccttcgctgt cccaaatcac aggaatggtc
     3841 atcaaatgac ccacgttgcg gataaacagc aacgagcgtc cgtgcagaga aatttcagag
     3901 ccatcggcgg cggtgtactg acgatcgtca ttcagtttac gtacgatttg ccgaccgttt
     3961 ttctccattt tctcttgcag agtcccctgc atcaggccca gcagattgcg gtacagcagg
     4021 attttatctt ccgcatcaac cgccgcgacc gaatcttcgc agtcgagaat ggtactgata
     4081 gcagcttcga cgataacatc gttgatgtgc gccggatcgt ctttgccaat ccgcccgttg
     4141 gcatcaattt gcagttcaat atgcaggccg ttatttttca gcaaaatgca ggtcggcgca
     4201 gcggcatcgc cacggtaacc gacaaactgt gctggagtac gtaacgtggt ttctttacca
     4261 tttttcaact ggatgcgtaa ttgtttatcg accaccttaa acgccaccac atcctgatag
     4321 ctgccgtttt ccagcggtag tgattcatcg aggaaacgtc gaacccaggc gataacctgc
     4381 tcaccgcgct gcggatcgta gccactgacc atcgcccctt cctgcgggat gatgtcgctg
     4441 ccgtataacg catcgtacag tgagccccag cgagcgttcg ccgcgttcag cgcgtagcgg
     4501 gcgttcattg ccggaaccac cagctgtggc cccgcctgac tggtgatttc gctgtcaatg
     4561 cccgtggttt ccaccgtcac gcgctccggt tgcggcacca ggtagcccag ttcacgaagg
     4621 aaagatttat aggccgcttt atcttttacc ggccccggat tgctgcgatg ccactcatca
     4681 agcgccgcct gaatgcgatc gcgttctgcc agcaactgac gattttctgg tgccagatcg
     4741 tgaacgatct catcaacatt gtgccagaat gcagcagcgt ccagttccac gcccggcaaa
     4801 acttctccat ccacaaaacg tttaaaattg gcgtcaatgc gtaaacggcc ctgggttatg
     4861 gtttgactca ttgtttctct cctcgttttc gcttatttcg ccaacaccgc tgctgccact
     4921 ttcgcgacct gcgcatcctg tgctccggtt aaaccagaaa cgcccacggc accaataatt
     4981 tgcccatcaa caacaaccgg tacgccgcct tccagcgacg tcagtaacgg cgcagtcacg
     5041 aacgcggtac gtccgttgtt caccatctct tcatagccct tagtttcacg acgccccagc
     5101 gcggcggtac gcgctttctc ctgggagatg taagccgcaa tcggcgcgca atcgtccatg
     5161 cgacttaacg ccagcagatg accgccgtca tcggcaacag caatggaaac agaccagtta
     5221 tttttctgcg cctcttcctg acctgcggca ataattgcac tcgccatttg ctggctaaga
     5281 atgactttag ttttcatttt tttattcctt ttcaagggct tgttctacaa tttcaatcca
     5341 gtgacgcaca gaggtacgac cggcgctcgc cagatgcgtc tggcaaccaa tgttggcggt
     5401 gacgatcatt tccggtttgc cgctttccag cgcattcatt ttgttatccc gcagctggcg
     5461 tgccagatcg ggatgcgtta acgcatatgt tcccgctgaa ccgcagcaca gatggctgtc
     5521 gggaacgtcc gttaaggtaa atccaagacg aagtaacact ttttccactt cgccgttcag
     5581 cttttgcgca tgttgtaggg tacacgggca gtggaaggcc agttttttat cgccgcgaat
     5641 tgccagtttt tccagcggtt cctcgcgcag aagttcgact aaatcgaccg ccagttcact
     5701 gacctgacgc gctttatcgg catataacgc atcgtttttc agcatctgcc catactcttt
     5761 gacgaacgcg ccgcaaccgc tggcggtttg caaaattgcc tcggcacctg cttcaatcgc
     5821 gggccaccag gcatcaatat tattgcgcgc ccgtgccaac cctttctcct gcgcattaag
     5881 atgatagtcc accgcgccac aacagcctgc ttcgttagct gacatgacgc tgatccccag
     5941 acgatccagc actcgcgcag ttgccgcgtt ggtgttgggc gaaagcgtag gctgggcgca
     6001 gccttccagc attaaaaccc gacgcttatg gcgcagcggc ggacgcggtt tagctttcac
     6061 cgtttcagca ggcagttttg ctctgacctg ttccggtaaa aacggtcgca gcaccagccc
     6121 tacctgcgtc agcgcacgga agaccgccgg acgcggcact acctggcgca atccttcgcg
     6181 cagcattcgc tccggcagtg ggcgtttcac tttctgctcg acaatatcac gcccgatatc
     6241 cagcaaattg tgatagcgca caccagaagg acaggtggtt tcacaattgc ggcaagtgag
     6301 gcagcgatcg agatgctcct gtgttttaag cgtgacttcg ttgccttcca gcacctgttt
     6361 aatcagatag atgcgcccgc gcggcccgtc cagttcatcg cccagaagct gataggttgg
     6421 gcaggttgcg gtacaaaatc cgcagtgaac acaggcgcac aggatgctgt cggcttccag
     6481 cgcgcgttct gccgcatctc ttcggttaat cgggtttgca tagcctgctc ctcaaagttc
     6541 cgcgtacatg cgaccggggt taaacacgcc gcaagggtcg agctgctgtt taagctgctg
     6601 gtgatagcgg aataaaggag ccgatagcgg ggcaaagcca ccatctccgg cactaaagcg
     6661 ggtcgcatga ccgccagcgt tgcgggcgat gcgatggatt tgattgtcat cggctgtcga
     6721 tttcagccag cgtaacgccc cgccccagtc gatcagttgc tcgccgggta aatccatcat
     6781 cggcgcatcg ctgggtaatg aaatgcgcca taaggtacct ggtaacgaga agaacggcag
     6841 ttgttgttca cgcaattgct gccagaactg acccgcaacc tcttcgccac ccagcagttc
     6901 acgcgctgct tttaccgatc cttcgccgcc ctcaaggcgg atccacaacg cattgtcgaa
     6961 gtaacataag ccactaatgg gtaatggctg gagttgccac tcggcgattt cactcatggc
     7021 ttcttgcagg ctgatttccc gacgcaggct cagagaggca cgcggtcgcg gtaacacttt
     7081 cattgagatt tcagtgagca cgccaagaca accatagctt ccggccatta accgtgagag
     7141 atcgtatccg gcaacgtttt tcatcacttc gccaccaaaa cgcagatgtt ttccagtgcc
     7201 ggtaatgatg cgcgtgccga ggacaaaatc gcggaccgaa ccgctccacg ggcgacgcgg
     7261 ccccgccagc ccgcaggcga ccatcccgcc ccaggtggct tcttcaccat aatgcggcgg
     7321 ctcgcaggga agcatttgcc cggcgctctc cagcgccgct tcaatcgcca ccagcggcgt
     7381 tccggcacga gcggttatca ccagctcggt cgggtcgtaa ttaacaatgc cgcgatgaca
     7441 acgcacatcc agcgtttgcc cggtgactgg gcgacctaaa aaggctttgc tattgctgcc
     7501 ctgaatcacc agcggcgttt tatcgctaat tgcctgattc acctgctcca gcagcgcctg
     7561 actgtaatca cactcgcgta gcatcaaaag cgctccagtt caggaaaagg taaatgaccg
     7621 tgatgcacat gcatggcacc aaattcagca cagcggtgta gcgtgggaat gtttttccca
     7681 gggttcagca aaccatcggg gtcaaacgcc gccttgaccg catggaaggt cgtgatttca
     7741 tcgctgttga actgggcgca catttgattg attttttctc gcccgatgcc atgttcccca
     7801 ctgatgctgc cgccaacttc aacgcagagt tcgaggatct tcccgcccag ctcttccgcg
     7861 cgggcaaatt caccaggttc gttggcatcg aaaaggatta acgggtgcat gttgccatct
     7921 ccggcatgaa agacgttggc aacacgtaaa tcatattgct gcgataaacg ggcaatgcct
     7981 tccagtacgc caggcagggc gcgacgcggg atggtgccat ccatgcagta gtaatccggg
     8041 gagatacgtc ctaccgccgg gaacgcattt ttgcgaccgg cccagaaacg tacgcgctct
     8101 gcttcgtcct gtgccaaacg gacgtcagtc gcgcccgctt tcaacaagat gtagttaacc
     8161 cgctcgcagt cttcctgtac gtcagactcc acgccgtcca gttcgcataa caaaatcgct
     8221 tcggcgtcga cgggataacc ggcatgaata aaatcttccg ccgcgcggat cgacaggtta
     8281 tccatcatct ccagcccgcc ggggataatg ccattggcga tgatgtcacc aaccgcaagt
     8341 ccggcttttt ctaccgagtc aaagctggct aacagaaccc gcgccacggg cggcttcggc
     8401 agcagtttta ccgtcacttc ggtggtcacg ccgagcatac cttccgatcc ggtgaacagc
     8461 gccagcaggt caaaaccagg tgaatccagc gcgtccgatc ccagcgtcag cgcctcgccg
     8521 tccagcgttt gcacttcaat tttcagcagg ttatgtacgg tcagaccata tttcaggcag
     8581 tggacgccac cggcattttc cgccacattg ccgccaattg aacaggcgat ttgtgaggaa
     8641 ggatccggcg cgtaatagag attatgcggc gcaacggcct aggagatcgc caggttacat
     8701 acgcccggct gcacgcgcgc gcggcgacca acggggttaa tgtcgaggct ctctttaaag
     8761 cgcgccatca ccaacaacac acctttttcc agcggcagcg cgccaccaga aagcccggtg
     8821 cctgcaccac gggtcaccac cggtacacgc aggcgatggc agacagccag aatcgctgtc
     8881 acctgttcca tttgcttagg cagaacaacc agtaatggac gcgtgcgata cgcgctcaac
     8941 ccgtcacact cgtaaggaat gatctcctca tcggtatgca ggatctcaag tccagggaca
     9001 tgctcacgca gtgccatcag taccgatgtg cggtcgacat cgggtaaagc gccatcaaga
     9061 cgctcttcgt acaagatgct catgagtagg cttcgctttg ttgtgttgtg tggcagctga
     9121 tttttgcgcg ctgcttctgt gaacagttat taagcgggct tttcgttttc gtctatctct
     9181 ttagctaccg gtcagaccat tttttttcca gctctgtgac cttgtcttgg ttaacttaat
     9241 gttaaattga tgtaacataa tcacttgcgt gatgtgcgtg ttttgcgagt taagaacaga
     9301 aaaattggtc ctacctgtgc acgaggtccg ggaatgaaag atgaacgtcg ccctatttgc
     9361 gaagtggttg cagagagtat cgaacggtta attatcgacg gcgtactgaa ggtcggtcag
     9421 ccgcttccct cggaacgtcg actgtgtgaa aagctcggct tctcacgctc cgcactgcgt
     9481 gaagggctga ccgtgctgcg cgggcgcggg attattgaaa cggcgcaggg tcgcgattct
     9541 catgtcgcac ggcttaatcg ggagcaggac accagcccgc tgatccatct gttcagtacg
     9601 cagccgcgaa cgctgtacga tctgctcgac gttcgcgcat tactggaggg cgaatcggca
     9661 aggctggcgg caacgctggg aacgcaggct gattttgttg tgataacccg ctgttatgaa
     9721 aaaatgctcg ccgccagtga gaaccacaaa gagatttcgc tgatcgaaca tgcgcagttg
     9781 gatcacgctt tccatctcgc catttgtcag gcttctcaca atcaggtgct ggtgtttacg
     9841 ctgcaatcat tgaccgatct gatgtttaat tcagtgtttg ccagcgtaaa taatctctac
     9901 catcgaccac agcaaaaaaa gcagatcgat cgccagcatg cgcggatcta caacgcggtg
     9961 ttgcagcggc tgccgcacgt cgctcagcgc gcagcgcgca gcgcgcagcg cgcagcgcgc
    10021 gatcatgtac ggaccgtgaa aaagaatctc cacgatatcg agctggaagg ccaccatttg
    10081 attcgctcgg cggtgccgct ggagatgaac aaggtgggaa tgtgagcaga gttttaccct
    10141 atccccaagg cgaggggacc gcccctgcac ttaacaggat ctggtgttaa atctgttcat
    10201 ctataccctc tcccgaatga ccgcccctgc gctaaacagg atctggtatt aaatttgctt
    10261 ttcatacaat cggtaacgct tgtacggctc cgccccaatg cgttccagca tgttattcat
    10321 gcctgtattg gtttcgagga tccatgacat ctccagcgca tcgatcttcc ggcgggcaaa
    10381 cggatcgcgt aaggcttcaa tcaataacag cgcaatcacc gggccgatgc ggctgaactg
    10441 atactcgtcg cgcacgccca tcagcggtac tcgcgcagtt cgcacaccgc tgactttcaa
    10501 acgccacagc aattttgccc agccgaaggg aaagagcgat ccgttcagat cggcaatcgc
    10561 ctcgttgatg ttaggcaagc cacaataaac gcgcagggtg cagaatcaat ctcagcgatg
    10621 tagatcatat cgtccggcac cagatattta agttggtcgc ccatggtcgc gaattcatgt
    10681 tcggtaaacg gcacaaatcc ccagttgtgc tgccagccag agttgaaaat ctcacgcagg
    10741 atctgcatct cttcggcaaa ccgctgacga ttgatgcagc gaatggtcac ctttttgcgc
    10801 acctgatcca tcagtttttt tagcgccgga gagaaagtga gatcggttcg ctgcatccac
    10861 cacgccagta aatcaatgcc tttgtgataa cccagttgtt caatatgcgc ggcataccac
    10921 ggtttgccgt gtggcatcat cgcacagggt ggtgtgtcaa aaccttcaat cagtaatccg
    10981 ctttcctgat tgatattcag gctgaaaggc ccgctgatct tacttgcacc ttgtgacttc
    11041 aaccacgctt ccgctgcgcc aaacaacgcg gcaaaatcct gcggatcatc aatggcgtca
    11101 atcatgccga aatgaccggt atcttcaccg taacgctcac ggtgcaaggt atctatttgc
    11161 gcggtaatac gcccaactat ctgccccgct ttttttgcca cccacgcctg ccagatgata
    11221 tggtccgtcc ccggattttt cgcagacaaa tgctcgttgc gttcaatgaa taaagggggt
    11281 atccagtttg gatcgtcggg ataaagtgat gacggaaaag cgataaatgc cttaaggtca
    11341 tttttattaa ttactttttc aattttaatc atcagcagat cgcattccat atctctgtgg
    11401 ataacaaata attaatggtg aaaaacaggt tgcaacagca acgagcggca tcttaataat
    11461 caactcaatg aatacaaacg acgaataaat agtattaatt aatgattatg ataaggtcag
    11521 aggctacaca gaaacgcctc aatgcctttt gtcaatttaa ggcgaccgga caaggcttct
    11581 tcttaatata ttcataaatt aaatatcatt cataataaaa acaaaaaact taaactcatt
    11641 atttaaatca atgttcattt tcacaccgat gaaaatacta tctcatttat cttttatgcc
    11701 tatataatcc accgcattat tgtcatttta tttttgatat ccaatttttt cgtggtgtat
    11761 atgtctaata aaatctttac gcattcccta cctatgcgct atgccgattt tccaacgctg
    11821 gttgatgctt tggactacgc cgctctgagt agagccggaa tgaattttta tgacagacgt
    11881 tgccaacttg aagatcaact ggaatatcag acattaaaaa cgcgtgccga agctggtgcg
    11941 aagcggttgt tatcgctgaa cctgaaaaaa ggcgaacgcg tggcgctgat tgccgaaacg
    12001 agtagcgggt ttgtagaggc tttttttgcc tgtcagtatg ctggcttagt cgccgtcccg
    12061 ttggcgattc caatgggcgt cggtcagcgg gattcctgga gcgccaaact ccagggttta
    12121 ctggcaagtt gccagcccgc agccattatc actggtgatg agtggttgcc actggtcaat
    12181 gccgcgacgc ataacaaccc cgaattacat gttttaagcc acgcttggtt taaggcatta
    12241 ccggaagccg atgttgcgct ccagcgtcca gttccgaacg atatcgccta cctccagtac
    12301 acctccggca gcacccgttt tccccgtggc gtcattatca cccatcgcga agtgatggct
    12361 aatctacgtg ctataagcca cgatggcatt aaattacgcc ctagcgaccg ctgcgtctcc
    12421 tggcagcctt tctaccatga tatgggactg gtcggctttc tcctgccccc cgtcgccacg
    12481 caactttcag tagattattt gcgcactcag gattttgcca tgcgtccttt gcaatggctt
    12541 aaattgatca gtaaaaatcg cggcaccgtt tccgttgcgc cgccgtttgg ctatgaattg
    12601 tgccaacgcc gcgtgaatga aaaagatctc gctgaactgg atctttcctg ctggcgcgtc
    12661 gctggtattg gtgctgagcc gatctccgca gaacaactcc atcaattcgc tgaatgtttc
    12721 cgtcaggtta actttgacaa taaaactttc atgccgtgct acggactggc agaaaatgcg
    12781 ctggctgtca gcttctctga tgaagcctcc ggggttgtgg ttaacgaagt ggatcgcgac
    12841 atcctcgaat atcagggtaa agccgtcgcg ccgggtgcag agacacgcgc cgtatcgact
    12901 ttcgtcaact gcggcaaagc gttgccggaa catggtattg aaatccgcaa tgaagcaggt
    12961 atgccggtcg cggaacgtgt ggtaggccat atttgcatct ccggtcccag tctgatgagc
    13021 ggttactttg gcgaccaggt ttcgcaagac gagattgccg cgacgggctg gttagacacc
    13081 ggcgacctcg gttatctgct ggacggttat ctgtatgtca ccggacgcat taaagatctg
    13141 attattattc gtggccgtaa tatctggccg caggatattg aatatatagc ggaacaggaa
    13201 ccggaaattc attctggcga tgcgattgct tttgttaccg cccaggaaaa aatcattttg
    13261 cagatccagt gtcggatcag cgacgaagaa cgtcgcgggc agcttatcca cgcgctggcg
    13321 gcacggatcc aaagcgaatt tggcgtgacc gcggctatcg atctgttgcc gccccacagt
    13381 attccccgaa cgtcctccgg caagcctgcc cgtgcggaag cgaaaaaacg ttatcagaag
    13441 gcttatgctg ccagtcttaa tgtgcaggaa tccctggcat aaatcaaact gtcgcggtga
    13501 cgggcgctac cgggtttatc ggtaaatata ttatcgataa cctgctcgcc cgcggctttc
    13561 acgttcgcgc attgacgcgt actgcccgcg ctcacgtcaa tgataatctc acctgggtgc
    13621 gcggttcgct ggaagatact cattcactta gcgagctggt tgccggagcc agcgtagtgg
    13681 tccattgcgc cgggcaagtg cgcgggcaca aagaagagat tttcacccgc tgtaacgttg
    13741 acggcagcct gcgcctgatg caagcagcaa aagagagcgg cttttgccaa cgttttctgt
    13801 ttatctcttc gctggcggcg cgccatcccg agctctcctg gtatgcaaaa tccaaatacg
    13861 tcgccgagca acgactggca gcaatggcgg acgaaattac gctgggcgtt tttcgcccga
    13921 cagccgtgta tggtcccggc gataaagagt taaaaccgct gtttgactgg atgctacgcg
    13981 gcctgctgcc acgacttggt gcgccagata cacaactctc ttttcttcac gtcaccgatt
    14041 ttgcgcaagc agtgggtcag tggttaagcg ccgaaactgt acagacgcaa acctatgaat
    14101 tatgcgatgg cgtcgccggt ggctatgact ggcaacgcgt acgacagctt gccgccaaag
    14161 cccgttgtgg ttccgtgcga atggttgaca ttcctctgcc ggtactcaca ggccttgcgg
    14221 atatcagtac cgcgttgagt cgcctggcag gtaaagaacc tatgctgacc cgctcgaaaa
    14281 ttcgtgaatt aacccacgcc gactggtcgg caagtaataa ccgtatttct gaagatatca
    14341 attggtttcc cgggattagc ctggaacacg cattacgcaa cgggctattt tgattgtctt
    14401 gttttaaagg tgtgttaacg tttctatggt aaatcgtgaa atagtaatgg attacatcct
    14461 ttcctgttta caggatttgg tcgaaaacgg tgtggaaatt aagcccgaca gcgatctcgt
    14521 caacgatctt ggcctggagt cgatcaaagt gatggatctc ttaatgatgc tggaagacag
    14581 attcgatatt tccattccta ttaatattct gctggatgtc aaaacccctg cgcaattaat
    14641 ggaaactcta cttccatggc tggagaataa ataatggggc tatacgataa atatgcgcgc
    14701 cttgctggcg agcgtctgca atttagtgac aacgggttaa caccgtttgg cacctgtatt
    14761 gatgaagtgt attccgccac cgaaggccgg atcggcaata aaaaagtgat tctggccggg
    14821 accaataatt atctcggttt aacctttaat catgatgcca tcagcgaagg tcaggccgcg
    14881 ttagcggccc aaggcaccgg caccaccggt tctcgtatgg caaacggcag ttatgcgccg
    14941 cacctggcgt tagagaaaga gattgccgag tttttcaatc tccctaccgc tatcgttttt
    15001 caaccggtta taccgctaat ttaggcgtca tcagcgcact tgccgaccat aacgccgtgg
    15061 tgttactgga tgccgacagc cacgccagta tttatgacgc ctgttctctc ggtggcgcgg
    15121 aaattattcg tttccgccat aacgatgcta aagatctgga acggcgcatg gttcgcctcg
    15181 gtgaacgggc gaaagaggcg attatcatcg ttgagggcat ctacagcatg ctcggtgacg
    15241 ttgcgccgct ggcggaaatt gttgatatca aacgtcgtct cggtggttat ttgattgtcg
    15301 atgaagcgca ttcgtttggc gtgttaggcg caacggggcg cggactggct gaagctgtcg
    15361 gcgttgaaga tgatgtggat attatcgttg gtactttcag taaaagtctg gcctcgattg
    15421 gcggttttgc tgtcggttcg gaagcgatgg aagtgctgcg ctacggcagc cgcccgtata
    15481 tttttaccgc ctctccttct ccgtcctgca ttgcgaccgt gcgctcgtcg ttaagaacta
    15541 ttgccacaca accagagctg cggcaaaagc tgatggataa cgccaaccat ctgtatgacg
    15601 gtctgcaaaa actgggttat gagttaagtt cacatattag cccggtagtg ccagtaataa
    15661 tcggttcgaa agaggaaggt ttacgcatct ggcgcgagct tatctcgccg ggcgtgtatg
    15721 tgaatcttat cctgccgcct gccgcgcctg caggcatcac gctgctgcgc tgtagcgtaa
    15781 atgctgccca cagtcgtgag caaatcgatg ccatcattca ggcatttgct actctgaagc
    15841 agtaatgtga gcccgccgtg gtgtttgcgc cacggcgggt ttaatgttgt ctgtcatacc
    15901 aatacaccag gctcaacgcc accaacacaa acgctaccgg taaaccgagc gcggtaacag
    15961 gtgcgcttaa cgcaaatagc aatcccagat taaccatgat ttgatagctg atccaggtga
    16021 gtaagcccac gatgacgcct acagcgagac ggctacccat cccaggcgaa cgcggggcac
    16081 taaaggtaaa cggcacagcc agcaaaatca tcgccagggt caggatgggt tgccccagtt
    16141 tttcccacag tgcaaggcgg tattcgctgc tgggttgccc ggtttcctgc aaataatgga
    16201 tgtaatgatt aagctgacgg acagaaaaag tattccccgg cattgataac tcttcaagat
    16261 tcatactggt gaaggccgat tgccaggcaa gattatctaa tgtttccagc gtttctttgc
    16321 cattcagcca ttttttatga ttcacaccat gcaggaccca cgttttgtcg tcttcaatgg
    16381 tggcactgcg tgcataaatg taggattcaa gcgagagatc gtcgcgataa tgaaatatct
    16441 ccacgcccac cggctggccc tgctcgttca gcgatttcac cgtcacaaat tcattaccac
    16501 gcctggccca cagcatattg ccggtaatgt catcgtcctc ccccaacgcg gtggcggtcg
    16561 atttgatttg caacgcctgt tgctgcaatg gcgacgccac ccactcatct atcgcgccta
    16621 acgaaacagt ccacaatatc ccggcaacca gcgccaccag tgcaatacgg aagatagaaa
    16681 accccgtgct gcgaatggcg gtaagctcac tgtttttcga taactgcccc agtccaacaa
    16741 taccacccaa taacgcgata aacggcgaaa gttcgaccag tgtgcgcggt aaggtcatta
    16801 gcaccaccag caccgcctga gtccagcgat aaccgcccgg gctgacacca tccagttcgt
    16861 taatcaggtt gaaggtggta aaaagcggca gcaatagccc tgcagcggcg gcaaaaccga
    16921 gaaagagatg acggattaaa tagcgactga aaacattcat cgggaaaatt tctgcagcaa
    16981 agagaagtca cgtgcgacca gtatcagcag gcccattagc atgagtccgg gcactaacca
    17041 caaaccagga atgaggggaa tcgcgccgtt agccaccagt gtacggcaga cgtcgccgcc
    17101 ataaaaaatg gcaataaaca gcaacgtcag tggcaataac gttgaaaatc gcccttgccg
    17161 cggttttacc cgacttaatg aaatggccag cagcgccatc aacaatgcgg tcaggccacg
    17221 gctttgtcgc cattgcagtt cggcatgatc ggcaggaaag gcggagcgcg ccagctccgt
    17281 gaccgatttc gctttgcgtt tgacgttcgg gctttgatcc agcggattca gatgtaattg
    17341 cagattacgg tagatctgtt cgttgtcgtc acgcccctga tgatcgagaa gataggcagt
    17401 gccgttgtgc aacataacgg tcggtttctc gggtgatggg tcaaccacat caaccgaacc
    17461 ggcgcggaaa atacgggttc gattggcagt agaagtataa atcagcgcgt cagtcaggcg
    17521 attattatcc tgatcaaccg tctgcgaaag gatcattcgt ccgttatcgt tagtgttaaa
    17581 tttctttgcc cgcaactggc gaacatccag ctccgactgc gactgctgct ccagttgata
    17641 aatctgcgca taagcccacg gacgaccata catcgacaga agggtgacaa aaatacttag
    17701 cggtatcgcc agataaagaa cggctttgta caagcgtccc ggactgcccc ccgccgcaga
    17761 gatagcggta atttccgagt cggtatacat ctgccctagc gtcacgccaa ctgacacata
    17821 cagaccaaca ggtaacaaca tctccagtgc aatcagtact ttgtagaaaa cgatatccag
    17881 cacaacatca agagctaatg tcccatttgc cgcttcggtc agataacgct gtgcggagta
    17941 gctggcgaaa ataaagatca gaaaaccgac gataatcagc accagacggc gcgtaccacg
    18001 catgatgtag tgttcaacca gcttcatctc actaacgccc tcaatccttt gagtttttta
    18061 ttcattgatc agtttctgcg ccaacgcaat gacatgatct acctcttccg gcgtcaggct
    18121 gccgtccttt gcccactgaa tttgcccggc tttattcagc accagaatgg ttgaactctg
    18181 ctccggcaag cgccaggcaa cgcgtccctg tccattgccg tcaataacga attgcgccca
    18241 cggatagcgg cgtttatttt tttcgatttt gccaacaaca aaatagccag taccaaagat
    18301 ggcatcatcg gtgttcacaa tggtggtggg ctgaaaacga tcctgcggaa agtttgccgc
    18361 ttcaaccgct ttaattaaca gtgagttctt tttcttcgcc gacttacgcc cggcgatata
    18421 ctgaataatg cggacttttc ccgctaattg tgtgctgttc cacttttggt agtgaatttc
    18481 gtcgtcgcta ttcagaagca gctcgccgcc ttcctggata taaacagggg taaccgtttt
    18541 gccagtgacg aaattgtgtg cctgcacacc cggggctaag agcatgataa tcaacgcaat
    18601 aatcaaacga gatgacatca ttgctccggc tatagcggaa aacgcgaaat cagcagctct
    18661 aagcgctgac aaatatcgct atccagcgcc aacggtgcat aatacgactg gcggttttcc
    18721 atatcatccg ccaccatgtc gtcgtgttta tctcccatca ggccattaat ggcggcgagg
    18781 aaacgggaaa tctgtaacgc ctctttattt tcaggatcaa gcgatcttaa ggtgtcgtaa
    18841 taagaaacct gctggctgat gatttttcgc acatcatgaa attccctgcc ggttagtcgc
    18901 ggtttggcaa taatctcttt cagggtatgc atttgctttt gccgataggc aataaaactg
    18961 cttacgcttt ctaactggct atggcgtaat gaatagttca ccaacgacca gaccggagag
    19021 cttaaataaa tccgtaacag gttgccgtaa tacgacacaa tatttttctt accgttacga
    19081 aagccgtcct gaaaacgccc cagaaaaacg gtcgttttgc cgaataaaaa tccggcctga
    19141 tgcttcttta aatacagccg ttgcgcaaaa cgcaggtgct tgtagcgcgc tcgcatgtat
    19201 ttaaattgtt tgcgctcatc agcagtcagc tcatcgcctt tggcttgttt gcgaactaaa
    19261 cgcagtaatt cctgacgatc aacgccattg agccagaatt gcaggcataa cgcgtaatta
    19321 tcatgaatga tttcggtggt acaacttagt gcaatagtcg gcggcaaact aacctgtgca
    19381 tcaacgacat catcttcttc aatcgcaaca aatagacgct cgctcgcctc aagcgggaaa
    19441 tattttctca tcatcaggat ttctctccgg ctgcgttata aagcaaggaa tggcgctgaa
    19501 aaaatgcctt taaggttgga attaacgaga gcgccacgtc cagacattgc ccaccgaagt
    19561 aagcaaacgc tgctcccagc acaccaaaca gatgacagaa catgaacagc aatacgatgt
    19621 agccgattga ggcgatggtt tgcgccacga gaaaagcgcg ctgttttccc gccatcaata
    19681 gcaatgactc ctgcgggaag cccagcatcg aaatcacaat tgcgcccagc atcacctgaa
    19741 tcaggtcata cgcttcgaga tacttaacgc caaacaccag cgaaatgagc ggcttgccga
    19801 cgatcaacac cgcgagagcc accagaatac cgattccgcc cgccagtaaa ccggacttca
    19861 cacctaacaa ccacggtctg gtggtgcgcg gatctaaacg catcacctct gggtaaaaac
    19921 ttttacccag caaacccgct ggcgttccgg cggcgtcgaa gaatgtcatg gcgattttaa
    19981 ataacccggc ggcagcgggt cctaacacga tccccaccaa cactgtgctg cacgagttac
    20041 gcgccgacca gatggagtgg gcaatgttgg ttgaccagac aaaactccac gcgcctttaa
    20101 tgtgtcgggc agactcaaac agattcaatt tgaaggcgtt atggatattt ctacggcgta
    20161 attcgcgcgc ggcaaaccac cagtacatgg tgccgccaac cagattcgac acgtaccagg
    20221 caataacaaa acccgcaaaa ccaaagtcaa aataccaggc tgcgacgctc cccgctgcgc
    20281 gcagaaaagg tttcgtcgcc tgctgtacag caatcaaatc aaagcgatct accgcacgca
    20341 gaatgccggt cggcgtggag gaagccattg aaggaatgag cgtgcaatac agcgccgcca
    20401 gccaaaagct ttggtcatcc agacctaatg aatgggaaag gaatggcagt aaggcaatgc
    20461 cgccgacaat cgccaccgcg ccgctgacga tatccagcga gaaggaaaat gagacgacat
    20521 tgcgaaattg ctgcggatta ttgttggtca aggctggcgt tccgtactga accaccagct
    20581 gccatgtctg aaacttaata aaatcgctga tcgacttggc gtacgattgc acaatcacca
    20641 gtacaccaaa catggcgggc gtcatccctt taccggcaca cgagagcgcc aacagcccca
    20701 gcaaggcgct ctcgacatta ctggagccta accaggcgct attgcgaata atggtgcgaa
    20761 acgcgccatc tgcaaaccaa tgtttgatgt taaaacccgc caattcagcc tgaccttatc
    20821 gttgatagta aaaaatatcc cgccagcctt aagttaaact tcggcggtca gaaacgatgg
    20881 caaccagaga aaccgccttc tgtacctgtt ccagcacttc gctgtagggc gctctggaat
    20941 caatctcaag aatttttgtg ccgttatagc caatcgtcga catgacgccg attttgtcct
    21001 gcagctcggc atactcatgg tcaggcttgc gggagatggc agtatcaata tcaatcccca
    21061 aacgaatgat taattcagga cggtattgcg ccattttttg gtataaactc cgttcgcgct
    21121 gcgccagaaa catgctgatt ttcccggtcg cacgttcgac gccgatcccc ggtccgtcat
    21181 aataaaagcc cgaaatttca gcctgcggga agcgatcgct gaccaccaga acgccacttt
    21241 gcgccagtcg ctgaaccttg cgtagattcg ccattcttcg cagcgagaag cagtacataa
    21301 tcaccgccgc ccatagcgcc ggagatttgg ttttcatgct ttgggttttc gaggatttgg
    21361 ccgccaatcg ccgttccagc cagacgccaa ccaacggcaa tcgtttgatt ttgtcgccgt
    21421 cttcgccgga gagcagcccc agatagcggc gctcggtttg ccagtgttgt tgcagcgatt
    21481 tcaccaggtc ggtggtcagc gtggatttac cggtgccatc acacccgacc accgcaatca
    21541 gccccggaat gtagttgggc tgcggcgcgg ttgtactatt aacagtttgt gtttgtagtg
    21601 catccattcg ttaaatcatc ccctatccat tttggtgatc aggaaagcgc ggcgtgaatt
    21661 gcgcgtagtg acgcttgcat aatttcatcg gctggttgcc gaccatccag ttcaaggatc
    21721 ttggcgccat tgaatgtcag ttgcggcgta acggcgattt tttcctgtaa cgctgccagt
    21781 tggtggtcag gtttacgcgc aaacgcggtt tgttcatcaa tgccaagacg aatcaacaat
    21841 acgggtaaat aagacgccat ccattggtac agcttcaact cgcgctgcct taacattttt
    21901 atccaaccgt taccgcccgt ggtttttgcc aattgcgggc catcaaagcg aaaacccggc
    21961 acttcaacct gcgggtagcg gtcggtgatg agcagatagc cttgctggct tttacacaac
    22021 attttgcgaa acttgtacgc ccgccagcag gaaagcagat agataaccag cgcagtaata
    22081 ttgccaggcg gagttaaggg cttttcgtgt acatgtgccg ctttacttcg cagataacac
    22141 ccacaaggtg cgccaataac agggagctgt gaaatccatt cgccaattcg cccggacgat
    22201 tgcccgagat aaatgtgttc tgttggcatt cttgctgcca gttcatttac caggcttgcc
    22261 gtgagggtcg atttacctga accatcacat cctacaatgg caataacacg cactggcgtg
    22321 gaattaatta ttgacatatt acgttgattc acgaaaaacc cggcagtaaa taatgtattg
    22381 aatattacgt ggtccgtgct cagaatatcc gttcaacctt attgtgacaa tggctaattg
    22441 tgacattgac ctgaattacg ctttcataaa aacatattaa ccaaataaat atttttaatg
    22501 gatatttaaa ttaaaggata tattcatgca gtcaataaca cctccattaa ttgccgttat
    22561 tggtagcgat ggttcaggca agtcaacggt gtgtgaacat cttattaccg ttgtcgaaaa
    22621 atatggtgct gccgaaagag ttcatttagg aaaacaggcc ggaaatgtcg gtcgtgcagt
    22681 gacaaaatta cctttgatgg gaaaatcctt acataaaaca attgaacgaa atcaggtgaa
    22741 aacagcaaaa aaattgcctg gaccagttcc ggcgctggta attacagcgt ttgtcgcccg
    22801 tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt cgcgggttaa ttgttctaac
    22861 cgaccgttat cctcaggacc aaattcctgg cgcttacgat ggtacggtgt tcccacctaa
    22921 cgttgaaggt ggtcgttttg tctcatggct ggcaagccag gaacgtaaag cgtttcactg
    22981 gatggcgagc cataagcctg atctggtcat caaactcaat gttgaccttg aagttgcctg
    23041 tgcacgtaaa cccgaccata aacgggaatc gctggcgagg aagattgcca taacgccaca
    23101 gttaaccttt ggtggtgcac aactggttga tatcgatgcc aatcagccac tggaacaggt
    23161 gttggttgat gcagaaaaag cgattacgga ttttatgacc gcgcgtggtt atcactagtc
    23221 aaaaatggaa atgcccgatc gccaggaccg ggcattttca ggaaggttaa atcaactgca
    23281 atgctatcca gtacagccca ccagaaagaa aaattgccgc cggtaaagta aacacccacg
    23341 ccatcaggat gctggttacc gttttacgct gtaacccacc gccgtccacc accatcgtcc
    23401 ctgctaccgc agacgagagg acgtgtgttg tggagacagg catcccaata taactggcaa
    23461 ggccgataga cactgccgcc gtcatttgtg ccgccatgcc ttgcgcatac gtcatgccgc
    23521 gcttaccaat cttctcaccg atggtcatcg ctacacgacg ccagccaatc atggtgccaa
    23581 tgccgagcgc cagtgctacc gccatgatga tccacaccgg agcgtactca atggtgctta
    23641 acatatcgct gcgcagtttt ttcagcaggt tctggtcttc tttactgacg cctggcagtt
    23701 tcgccagctt cgcggaggta tcggagatgc acagcataat gcggcgcagc tggctgcgct
    23761 gactcacgct taacggctcg taactttcca tattgcctgg cagcatcgtt ttaacgcgcg
    23821 caatcgcatc aaaggtattt gccggatgac agtgaaactc tgttacttgc gtgccatcag
    23881 tcgatgctgc aggcaatgga ggttccatcg caatcaactt ctgcggcagt tcaggatgct
    23941 gttgcaggta gtgttcgaag ttggtaacgg catcgcgggt acgggtaatt tcatagccgg
    24001 acgcattcat attgacgacg aagccagcag gggcaatccc caccagtacc agcattacca
    24061 ggccgatccc tttctgcccg tcgttcgcgc cgtgcgaaaa cgccacgccc gcagcggaaa
    24121 caatcagcgc aatacgcgtc cagaatggcg gtttacgttt gccctttttc tttttgcgat
    24181 cttccggaat gcggtgaata cggtcacgct ttttcgtccc actccagaaa cggcgcagca
    24241 ggaaaatcag accacccgca atgaccaggc cgacgatagg ggaaacaatc agcgaggaga
    24301 aaattttggt tacttcacgc aggtttaacg catccatcac cgatgagccg gttaacagcg
    24361 cgttggttaa accgatgccg ataatcgcac caatcaaggt gtgcgaactg gaggccggta
    24421 aaccaaagaa ccacgttccc aggttccaga taatcgccgc cagcagcatg gaaaagacca
    24481 tcgccaaacc gtgagttgac cccatattca gcaacaaatc gattggtaac atatggacaa
    24541 tggcataggc aacgctaagt ccgcccaata acacgccaaa aaagttaaaa aatgccgcca
    24601 tcaccacagc aagttgtggc tgcatggcac gagtataaat aacggttgcc accgcattcg
    24661 ccgtgtcatg aaaaccattg attgcttcgt agaacaacac aaatgccaga gcaagtaata
    24721 acaaaagccc cgtatatata tcaaggccaa caaataaatt tagcatatat tagattacgc
    24781 cattttgaat ttacgaacgg acgcattatc agtgacttta acggcatggg caaagtgaaa
    24841 tatcattttt taacgataaa aagagtgtta tttcttgtgt atcagacaat aagcattcat
    24901 tcaaaacctt tagaaataat caaaacacag gtattccact ggttaatata ttcacatatg
    24961 aaatgaataa atattggaat atataaatat tgaatatttt gattaatccc cgtactaatt
    25021 attcttcata atcagtacgg ggttgctaca acatgtatta ctttttcacc acaattaacg
    25081 gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa cacgtcccac
    25141 cgtagttgcc gccaacggtg aaggtacata cctgaatgta tttaccgtcc actttcggca
    25201 aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccgctg gttttgtcca
    25261 gcacctcttc atgatggctg accaggtcga tattgctacc acagcgaccg gcgatcggtt
    25321 tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta tccagcaggt
    25381 aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg ccggggatca
    25441 ccgtccacag cggctcaaag accagcactt ccgggcgcag caatacgtcg ataagacgca
    25501 cttcgttttg cggatgaccg gtacggattg gcaccgcagc aaactcacgg tcgctaactt
    25561 cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag ttaaccagtc
    25621 gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc agcccgcgca
    25681 agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc gcgtgatagt
    25741 tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga ctgtgtttcc
    25801 aggcaccagc cagttcgtta atcagccctt ccgccggatt gaagccgttg cctttatagc
    25861 cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag gcggagtcgg
    25921 cgttgtattc gtaaaccttc aggccacgct catccatgca gaaatccata cgaccagtga
    25981 tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg attttcggaa
    26041 tgtcgaacag cgccagcagg ttgtcatctt tcagcacctt gtcggttgcg tgaagataca
    26101 tcagatgcag ctcgttggtg gctttaatta gctcctgctc ggcactctcg gtaatggtgt
    26161 agtaatgata cggatcctga ttgatcacct gaccgttggc ctgcacatag gcgttttgta
    26221 gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc agacgcgctc
    26281 cactgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat tcagtatctt
    26341 ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtcttta agggtatagc
    26401 agccgttttc gaccaccatc tccagctcgc gcgtccactg ttgcccttgc ggcaacgggg
    26461 aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg gtaatgatgg
    26521 cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc gcacccgcga
    26581 ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg tcattaacca
    26641 cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca gtaaagacca
    26701 caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac ttgtggccca
    26761 tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc tgcggatcga
    26821 gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc aataatgtcc
    26881 cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta acaatacaac
    26941 ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt aaccggacag
    27001 aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa aaaattcaca
    27061 ttctgtacag caagtgacct gctacactgc ttcaacacta ccactcagaa ggcaactcac
    27121 tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat ccgctggcgg
    27181 cgcgttcgcc aatatcaatc gcccgttttc tggtccgacg catgaaaaaa cgctgcccgt
    27241 tggcaaacat ccattgcaac tttattcgct gggaacgccg aacggtcaga aagtaacgat
    27301 tatgcttgag gagctactgg cgctgggcgt tactggtgca gagtacgacg cctggctgat
    27361 tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc caaactcgaa
    27421 gatcccggcg ctgcgcgatc atacgcataa tccgccgatc cgcgtgtttg aatctggttc
    27481 gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatc tggcaaaacg
    27541 gactgaaacg ttgaactggc tgttctggtt acagggtgcc gcgccgttcc tcggcggtgg
    27601 ttttggtcac ttttaccatt acgcgccggt gaaaattgag tacgccatca accgctttac
    27661 catggaagcc aagcgcctgc tcgacgtgct ggataagcaa ctggcgcagc ataagtttgt
    27721 tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg gcaacgtggt
    27781 gctcggcggc gtgtatgatg ccgcagagtt tctggatgcg ggaagttata aacatgtgca
    27841 acgctgggcg aaagaagtgg gcgaacgtcc ggcagtgaaa cgtgggcgta ttgttaaccg
    27901 caccaacggg ccgctgaacg agcagttgca tgagcgccat gacgccagtg attttgagac
    27961 aaatacagaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa gccatccagg
    28021 ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg cgacgctgcc
    28081 gcgtcttatc aggcctacaa aagctaatca tcacgcgctg gtaatgtcgt aatccatttg
    28141 gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca tggcaaatcc
    28201 gacgtgtacc agcacccact ggcccagcag atcggcaggg ttaccttcgc aaatcagggc
    28261 gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt gaatatcttc
    28321 accgacagcc agcacctggc ctggaacgcc aatacacatt gttaactccg gttatttcga
    28381 ttgccggaca aacgcgaaaa agaaccttta gcgggtgttc cctctcaccc taaccctctc
    28441 cccggagggg cgaggggacc gtccgtgcgc tttttaggcg ttgtcctcag tcttattcaa
    28501 cttcaatact tttgacgatc agcgaatcgc cggtatcgac acgcaaccgc tcgccgtgac
    28561 agagcggaca ctgcgcatcg tgctgatgaa tctccaccac ctggctgcaa tcccagcacc
    28621 aggcctgggc gggtttatag acgatatgta aatcgcaccc ttgcgccacc gttccgtggc
    28681 agacaatttc aaaactaaaa cggacggcgc tctcctcaac gcaggagagc gcgccaattt
    28741 ccagccacac ggcggtgacg cgcttaacat cgtgctgctc cgcctgccgt tggataattt
    28801 caacggcgct ctggcaaagc gacaactcat gcattttcgc tcctcctgcg gccaaacagc
    28861 agggcgcgac gccctgcgtg tggtacatcc ggattggtga ctggcaacga aaggatcatt
    28921 cgtgcgcagt catccgtcag gcgttggcct tcttcaatcg acatgctgtg cgaaagcggc
    28981 gacatcagcg aacaggagag gtattgcgaa acaccgtcca gttcgccaac ggtaaaggtc
    29041 atggtgccat acggcagttg cagaccaatt ttttcactca ctttgcgcag cggccagagt
    29101 tgatccgggc cggggaagat aactgcactc agcatccacg gggtgatcac acaccccgtc
    29161 cactgacctt cgaacagcgt aaaatcagaa acatacaccg gcattgaagg atgcagaaaa
    29221 gagagatcgt gcatcgaacg ccgggcaatt tcttcaaacg ctgcctgtac ttgcgccttc
    29281 ggggaggtct ggaaacctgc tatctcttca gtcatgaatc gcctcccgtg ggatggcttc
    29341 cacgcctgat tcgcgcagcg cagccagaac ctgctcaagc gcaggttcaa tcattgcttc
    29401 aaccgtcggc gttaagccga tgtgtggctc cagcgattcc gggatcacgc cgaccagggt
    29461 cagctttttc ggaaactcac cggtgaagcg cagggccgac aatacgtcgg ccaggccaag
    29521 ctgatgcgga gagattttgt tggtaaacaa cgccggaact tcttcatccc gcaggatcat
    29581 catcgttccc ggagtgttct ttttcgacac aatggcatcc gcaataatca gatgatcgcg
    29641 atttgccatg tcgccaagca gctccattcc cgccgtgccg ccatcgagga tctcaacata
    29701 atccggcaga atgtatcgtt gctctaacgc ttcgacaatc cgcacgccga tggcttcatc
    29761 ggtcagcaaa atattgccga cccctaagac taaaatacgc attacagaac cttcactgaa
    29821 accacttcgt tgccgtcggc atccactacg tgtaccgcac aggccatgca cgggtcgaag
    29881 gagtgaatgg tacgcaccac ttccagcggt ttattcggat cggcaaccgg tgtacccacc
    29941 agcgactgct cgtaaggacc gacgtcatca ttgaagttac gcggaccaga gttccaggtt
    30001 gatggaacaa ccgcctggta gttgctgatg ataccgtctt taataaccat ccagtgagag
    30061 agcataccgc gcggcgcttc gaggaagcca acacctttga actcacccgt tgccggaatg
    30121 ttcggcttca caaaggtggt gtgatcgcct ttgccgatat tggtgatcag tgcactgtat
    30181 tggttttgca ggatatcctg caattcacag cagtgaacgg tacgaccaat aatacggccc
    30241 agcgtggagt gcagctgtgc cacttccagc gtgttgccag tcagtttctg ataaatcgca
    30301 acgatttcat tcagtttgtt ctgggtagat tcgcgacctg ccgccagttt caccagcata
    30361 ttagccagcg gacccacttc taccgttttg ccgtagaaag tcggtgattt cacccaggaa
    30421 tatttcccgt cgtcagacca accatcataa gccggaatgg tggtaccttc ccacggcgcc
    30481 tgtggcgctt cgtctttata ccaggagtgc ttcgcgcttt cctgaatgcc tttgatcagg
    30541 tattcatcgg aatgagaagt gatcggacga tacgaggaca gatccgcatt ctcaatgtag
    30601 cctcccggga acaggaagct gccgtttttg ctgtcggtcg ggaattccgg cacgctcagg
    30661 tagttcaccg cacctttacc gcgcgtcagc cattccgggt agaacgcggc gataaccgcg
    30721 gtatcaacct tataaacctg ctcaacaaag tcgctcagtt tgtcgatgaa agacttgatg
    30781 tacatcaggc gctcaaggtt caacacgccc aacccgtcga ggttgattgg gttcgcgaca
    30841 ccacccactg ccaggttctg aatgtgcggc gttttaccgc ccagcagcgc cacgacgcgg
    30901 ttagcgtcac gctggcactc cagcgcctgc aggtagtgcg ctaccgcaat caggttcact
    30961 tccggcggca gtttcatcgc cgggtgaccc cagtagccat tggcgaaaat acccaactga
    31021 ccgctggcaa ccagatcttt gatcttgttc tgaactttag tgaactcttc cggactgttc
    31081 agatgccagg tcgaaacgcc tttcagcatt tcggaggctt tggttgggtc agcttgcagt
    31141 gcagaagtga tgtccaccca gtccagcgcc gaaagctgat agaaatgaac gatatggtca
    31201 tgcgtggtgt gcgcagccag aatgatgtta cggatgtatt gcgcgttaac cggaacgtcg
    31261 atattcagcg cactttctgc cgcacgaacg gaagacagcg cgtgagtggt agtacatacg
    31321 ccacagatac gttgcacaat catccatgca tcgcgcggat cgcggttttt cacgatctct
    31381 tccatgccgc gccacatggt accggaagcc catgctttcg aaacgacgcc attttcgatt
    31441 tcgcaatcga tgcgtaaatg cccctcaata cgggttaccg gatcaatagt aattctctgg
    31501 ctcatgcttt gctcgcctca tgacgattat gatcgttttg ttttaaagga ggaagtatcg
    31561 gcagtagacg aatgagtacg atgtaagcgc aaatctcaat agccacaaaa ccaatagaaa
    31621 tcaacagttc ttcccaggtc gggaagtagg cgtaaccgcc gcccgggttg aatgccacca
    31681 acgaataggt cagacgccag gttgcacaac ctaacagtgc gctcagtgct gacaggaaca
    31741 gcatgcggga atcattacgc agcttcgcca cacgcagaac gaccagcggg aagagcatca
    31801 gcaggacttc aatccagaac atcacggagt agaagtcacc ggcaaacgct aacgacagct
    31861 tgtcgcgata gataagctcg ccaaagcgca gcccgatgaa aatcgccagc aacacactga
    31921 tggtgttggt cagcttgacg aacaggctct tttcatccgg accgttgcca cgcagacccg
    31981 cctgcaccag tgaaccttca aagatgacaa tcgagaagcc catgatgaac gccgtcagca
    32041 gcgagaacag cggcaacatt tcatagctct gccagagcgg atgcaccttg tagcccgccg
    32101 agatcatcag cgaccccatt gaagactggt gcatggtcgg cagcagcgca ccgagcgcga
    32161 tgatgaagaa catcaccttg ttcagacgtt gtagcgacac cttccagccc agacgttcaa
    32221 acagtgccgg agcaaactcc agagccatca cgccgatgta gatggtcata cagaccgccg
    32281 tctcgaacag taccgagttc acgttgaagt gacccggaat gtagaagtac ggcaggttcc
    32341 agtagcgacc cacgtcgata gtgatcgaca agccacccag tgagtaacca aacagactcg
    32401 ccaacagcgc cggacgcacc agcggatggt attgcccacg gttaaagacg tataccgccc
    32461 acgccagcgc ccagccgcca caggcaaagc cggtgccaat cagcaggtca aacgcgatcc
    32521 acacgcccca cgggaagccg ccgttcaggt cagagacaga gcccagaccg aacaccagac
    32581 gcttcacaat caggagcata cagatgacga ttaacggtcc aaaaatcatg accggtttac
    32641 tgatgatttt gccgcccagc ggttgtggat catgactcat gatcgtctcc tccgtcgtga
    32701 tggtcgtttt tggtgttgcg acgaaccagc acggttaagc ccgccagcac agccagtggt
    32761 agcatcatgc ctttatacag ggtgtgttga atattttcgg aacgcgcacc ggtagaaaga
    32821 tcgtccagtt tcggcaggtc gagattttca taaggcacac ccgtcagtac cagaacctga
    32881 gtaccgccgc cctctttctc gccgtacaga tgcggataat atttcggcac cgtatgcagg
    32941 taagtgtcgc cagatttcag cgtctgacgc ggatagtggt attcgctgcc aggcttcagc
    33001 gccagacgtt ttttcgcctc cgccatcagc tcttcacgcg taccgaaaat caccgcgccc
    33061 gccgggcaca cttctacgca gccaggcaga ccgcctttat cgagacgttc cacacctttc
    33121 tggttgcaca gctcgcactt atgcagcgca ccaaacgggt tgttgtagtc gtacttcggc
    33181 acgttgtacg gacaggcgac catgcagtaa cggcaaccgg tgcacacgtc tttgtcgtaa
    33241 tggacaatgc cggttttcgg atcttttttc agtgcagaga ccgggcacac agagacacag
    33301 ttcggatcga cgcagtgcat acactgtttc ttaatgtacg cgtagccgtt ctcctcctgg
    33361 tctttgttga cccctgtgcc gctggtccac acctgaatga tgttattggt atacggcgac
    33421 agtttgtcgt tgttcgacca ggtctgttcc ccttgcgggt tacgttcagg gaagttgata
    33481 tcctgacact tggtgacgca agcctggcag cctacgcaca aggtcgagtc gtacaacatc
    33541 cccagcgatc ccggaattgg cgggcggttt tcagcagccg catgactgac agacggcagc
    33601 gcgcccgtca gcaatgcccc gcaggaggct gctttaataa aattacgtct gttcacggtt
    33661 attctccccg tgagtcagcg ttatctttct tttgctgacg acccagttca cgcaccgcca
    33721 tcacgctgac accggcaacc agcccaacca caccgccgag caaaccaata gcgcctgcag
    33781 agacgttgcc gccctcttta gcgttaacat ccggtttctg tgaacgcgga gtctgatttt
    33841 cgacgttggc aagctgatgg atgcctttat ggaagccgat acctttttcg ttacagccat
    33901 agcacgggtg accaatcgcc accggccaca caccgccaac atcgcagaat tgcagcgttg
    33961 agcagttgcc gtaagtttct ggccctttac agccgaggtg gtacaggcac cagccttcgc
    34021 ggtggccttc atcaccgaac tctttggcaa aacgaccagc atcgaagtgc gggcgacgtt
    34081 cgcagtgttc gtgaatcaga cggccatagg cgaaggtcgg acggtttttg tcatccagtt
    34141 tcggcggttt gccgtaagtg atgatgtgcg caacggtcgc gaggaagttg tgcgggttcg
    34201 gcgggcagcc cggaatattg ataacggttt tgcctggcag aacttcttgc aagctgactg
    34261 cgccagttgg gttaactcca gctgcggcaa caccgcccca cgcggagcag gaaccaatag
    34321 cgataatggc tgctgagcct tccgccgctt tgcggatgtg atccacaatc ggctcaccgg
    34381 caaccatgca ataaataccg ttatctttta atgggatgga accatccacc actaacacat
    34441 actgcccttt gtacttctcg agagcgttat gtttgttctc ttcgacctga tgaccgaagg
    34501 cggcggaaag cacttcgtga tattccagag agatagtctc cagcacgagg ttttctaccg
    34561 ttggatgcgt tgcacgaagc agagattccg tacaaccggt gcactcctgc gcgccaatcc
    34621 agataactgg cggacgctgc gggttagtaa ccgattcggc catctctgca gcggctttgc
    34681 tacttaaccc catggtggcg gctaatgctg cacaaagctt catgaaatca cgacggttaa
    34741 tgccgtgaga atggatgagg gtgttatctc cagtcattta tagttattcc gttgcgagga
    34801 cctggcatat attttgcctc aatcgcaaaa tcaataatgc gatcgatgcg ccatttacca
    34861 cacatttatt atggttatcg atatcatgat actgcggcgg ggcgctaaaa cgaagggaag
    34921 aagcattagt gtaattaatt aaagcaagat aatacgtatg tttgatcaat tttcgaggca
    34981 ataaccggcc ataaaaaagc gcaacgtaga acaggaatta ttctcacgaa agaaatccat
    35041 taccatctct ttatccagtc catagcggcg cgtcagaata cccgcttccc aggcgctaag
    35101 ctggcgatct ccggtctttt cgtacatccg gtgtgagtag cctaatacaa aaccgcgttt
    35161 ataatcggca cagaattttg tcacattaac cgcactgtcg gcatgcgttg cttttaatcc
    35221 cgccattaag cctttaccaa aatggttatt catcgttcac ctcattcgtt atatatcgcc
    35281 atatatagcg atattttagg caaagaacga cttttgcgca cgctattttt tcatacttgt
    35341 ttaagacgta atcccgtaag agataaacct atcaggagag atatttatcg aaccatttca
    35401 gtgtcctttg ccaggcaaga tcggcggcag atttgtcata acggggcgtg gaatcattat
    35461 ggaatccgtg attaaccccc ggatagatat aagcctcata aaccttatta ttgtctttca
    35521 acgccgcctc gtaaggaggc cagccctcgt tgattcgggt gtccagttcc gcgaagtgga
    35581 gtagtaaagg cgcttcaatc ttcgccacat cggcagtggg tgcctgacga ccataaaacg
    35641 gcaccgcgca ggccagttcc ggatacgcga cagccgccgc gttcgatacg ccaccgccat
    35701 agcaaaatcc ggtaataccc actttacctg tcgcttgcgg atagcgttgc ataaactcaa
    35761 ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac ctgctgttgc agctcacgac
    35821 ctttatcatc atttcccgga taacctccaa cggaacttaa tccgtcaggt gccagggcga
    35881 tataccccgc cttcgccact cgccgtgcca catcttcgat atacggattc agtccacgat
    35941 tctcatgcac cacccccacg gctggcgttt tgccgctcat ttttgcgggt ttcaccagat
    36001 aaccccgtac ctcgccgtga ccatttggcg aaggatacgt gatgtactca gcaacaatct
    36061 ccgggtcggt aaactctacc tgagtcgcca gcgcataatt tggcttgagc aaatcaaaca
    36121 acgctaatgc cgtcatcccg cccaccgcat acttcgccgc aaggttgagg aactcacgtt
    36181 tcgaaatttt cccgtgaacg taatagtcgt agtaatccaa caactcttgt gggaaatctt
    36241 tggcggttaa acgcggcatc gttgcattcc tcaactggtg tttttttaag caaagcataa
    36301 gcgcgtgttt ttgcccagtt tttcgtcact ctgtgagcca gactacggga tacgcgctgg
    36361 cgaatcgcta aactagaaac attgtttcga aattgaacgg aggaaaggag aggtcatggt
    36421 ctggttagcg aatcccgaac gttacgggca gatgcagtac cgctattgcg gaaaaagtgg
    36481 tttacgcctg cccgcattat cgctcggttt atggcacaat ttcggtcacg ttaacgcgct
    36541 ggaatcacag cgtgcaatcc tgcgtaaagc gtttgattta ggcattacgc actttgattt
    36601 agccaacaat tacgggccgc ctccaggaag cgcagaagag aactttggtc gcctgctgcg
    36661 cgaggatttt gctgcttatc gcgatgaact gattatctct accaaggctg gctacgatat
    36721 gtggcccggc ccgtacggct ctggcggttc acgtaaatac ctgctcgcca gcctcgacca
    36781 aagcctgaag cgtatggggc ttgagtatgt cgatatcttt tactctcatc gcgtcgatga
    36841 aaatacgccg atggaagaaa ccgcctctgc gctggctcat gcggtacaaa gcggtaaggc
    36901 gctgtatgtc gggatctcct cttactcgcc agagcggacg caaaaaatgg tcgagttgct
    36961 gcacgagtgg aaaattccgc ttttaattca tcaaccttcg tacaatttac tgaaccgctg
    37021 ggtggataaa agcggcctgc tggataccct gcaaaataac ggcgtgggct gtattgcctt
    37081 tactcctctg gctcagggat tgctgaccgg aaaatatctc aacggcattc cgcaagattc
    37141 acggatgcat cgtgaaggga ataaagttcg tggtctgacg ccgaaaatgc ttaccgaagc
    37201 caacctcaac agcctgcgct tattgaatga aatggcacag cagcgtggac aatcaatggc
    37261 gcaaatggcg ttaagctggt tgctgaaaga tgatcgcgtg acgtcggtat tgattggtgc
    37321 cagccgcgcg gagcaactag aggagaacgt gcaggcgctg aataatctga catttagcac
    37381 cgaggagctg gcgcagattg atcagcatat cgccgatggc gagctgaatc tgtggcaggc
    37441 gtcgtccgat aaatgacctg ttaataacgg gcgcgcgaac cgcgcccgaa tcagatcagt
    37501 gattatgacg agtcagtcgg tcaagatagc ccatcacaaa tgcagaaagc acaaacgtca
    37561 gatggataat gacgtaccac atcagtttgt tatccgggac atttttcgca tccataaaga
    37621 cgcgcagtaa gtgaatggaa gaaattgcca caatcgacgc tgctactttg tttttcagcg
    37681 acgttgcgtc cattttcccc agccacttca gcttctcttt gttctcggag atatccagct
    37741 gtgagacgaa attctcataa ccggaaaaca tcaccatcac cagcaaaccg ccaaccagcg
    37801 tcatatccac cagcgacagc aacacgagga tcaaatctga ttccgccatc gagaagatat
    37861 tcggcagtac gtgaataatc tcctggaaga acttcagcgc cagggcaact aacgccagcg
    37921 aaaggccaaa gtacacgggg gcaagcagcc agcgagaagc atacattgca ttttcaagaa
    37981 aacgttccat acattcctgt cgtgtagtta aaacgggcaa cagtatatcg caattgagta
    38041 aacagcacgc aacagcaaat caacatatac gttaaacctt tgtcgcataa ttctgaggtt
    38101 tagggatctt cctggttccc ttatcaacgc atcgagagcg tctaccctta aaggacgttg
    38161 aacctgaagg gagaaaaacg atgtctcatt taaaagaccc gaccacgcag tattacactg
    38221 gtgaatatcc caaacagaaa caaccgacgc caggcatcca ggcgaagatg acaccgatac
    38281 cggattgcgg cgagaaaacc tatgttggta gcggtcgcct gaaagatcgt aaagcactgg
    38341 tgacaggggg cgattccgga ataggtcgcg ctgccgccat cgcttacgcg cgtgaagggg
    38401 ctgacgtggc gatcagttat cttcccgtgg aagaagaaga cgctcaggat gtgaaaaaga
    38461 tcattgaaga atgcggacgc aaagccgttc tgctgccagg cgatttaagc gatgagaaat
    38521 tcgcccgttc gctggttcac gaagcgcaca aggcgttagg cgggctggat attatggcgc
    38581 tggtcgccgg gaaacaggtt gccattccgg atattgcaga cctcaccagc gaacagtttc
    38641 aaaagacctt tgccattaac gttttcgcgc tgttctggct aacccaggaa gcgatccccc
    38701 tgctaccgaa aggtgcaagt gtcatcacca cttcgtcaat ccaggcatac cagccaagtc
    38761 cgcatttact ggactatgcg gctacgaagg cggcgattct gaactacagc cgtggcctgg
    38821 caaaacaggt cgcggagaaa ggtattcggg tgaatattgt cgcgccaggc cctatctgga
    38881 cggcactgca aatttccggc ggacaaacgc aggataagat cccgcagttt ggtcagaaaa
    38941 cgccgatgaa gcgtgcgggg caaccggcgg aactggcccc tgtatatgtt tatctggcaa
    39001 gtcaggagtc gagctacgtc accgcagaag tgcacggcgt gtgcggcggc gagcatttag
    39061 gttaaaaaaa tgcccggttg tgaaaagcaa ccgggcatca ttgtgaattc actcttacct
    39121 gatgtggcga aaacgcctta tccgcctaca aacgcatgaa gattcaacat gttgcaatag
    39181 attttgtagg cctgataagc gtagcgcatc aggcattgag cacctaatgc ctgatgcgtc
    39241 gtgaacgcct tatccggcct acaaacgcac gaagattcaa catgttgcaa tagattttgt
    39301 aggcctgatg agcgtagcgc atcaggcaat tctactttac ttcgctttgg cggtttcttc
    39361 gccgaccaga cctatcttca ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa
    39421 cgtctcgtaa tcgacggttt tgtccgctcg gaagaagatg gtggtgtctt tcttgccttc
    39481 ggttaacgca ttcaacgccg taatcattgt ttcatcggtg accggatcgt taccgataaa
    39541 catcgagttg tctgccttca ccgacagata aaccggtttt tccgggcgcg gctgcggcgt
    39601 gctggtagaa gcaggcaagt tcaccttcac atctaccgtc gctaacggtg ccgccaccat
    39661 aaagataatc agcagaacca acatcacgtc gataaacggc gtcacgttga tatcatgcat
    39721 ttcgccgtta tcgtcgaggt tttcgttaag atgcattgcc atcggacatt atcctgcgcg
    39781 taatttttgt gcgacacgaa ccggatgcgc agcggcgctg gcttccagat ccaggtcacg
    39841 gctttgcagc aacaatacct gcgctgcaac atcacccagc atcgctttaa agccgccaat
    39901 ctggcgtgca aatacgttat agataacgac cgcaggaatc gctgccacga ggccgattgc
    39961 cgttgctaac agagcttctg cgatacccgg cgcaacgact gccaggttag tggtctgcgt
    40021 ttgcgcgata ccaataaagc tgttcatgat gccccagacc gtaccaaaca gaccaacgaa
    40081 cggagaaatc gcgccgatgg ttgccagata gccgttaccg cggcccattt gacgacccac
    40141 tgcggcgacc cgacgctcca gacggaagct ggtacgttct ttaatacctt cgttatcgtc
    40201 gctgccttct gacagttcca gctcattctg cgcttcattg agcaaatgca ggcttaagct
    40261 tttgctacca aaatcagcgg cgatatcgtt ggcctggttt aaggaacgtg cttcagccag
    40321 cagttgctgc tcgcgcttaa ggcgacgctt ctgattgaag aactctacgc tcttactgaa
    40381 gaagattgcc caggtgacta cagaggccaa aataagccca atcatcacgc acttaacgac
    40441 aatatcggcg tactgataca taccccagac ggaaaggtcc gtctgcatta aattattacc
    40501 cactgtgtat ctccaggacg caagtcacaa aatctgcgca taataatatc aaaacgacgt
    40561 cgaattgata gtcgttctca ttactatttg catactgccg tacctttgct ttcttttcct
    40621 tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc gaaaattttc tgctttatgc
    40681 caattcttca ggatgcgccc gcgaatattc atgctagttt agacatccag acgtataaaa
    40741 acaggaatcc cgacatggcg gacaaaaagc ttgatactca actggtgaat gcaggacgca
    40801 gcaaaaaata cactcttggc gcggtaaata gcgtgattca gcgcgcttct tcgctggtat
    40861 ttgagagtat ggaagccaaa aagcacgcga cgcgcaatcg cgccaatggc gagttgttct
    40921 atggacggcg cggaacgtta acccattttt ccttacaaca agcgatgtgt gaactggaag
    40981 gtggcgcagg ctgcgcgcta tttccctgcg gggcggcagc ggttgctaat tcgattcttg
    41041 cttttgtcga acagggcgat catgtgttga tgaccaacac cgcctatgaa ccgagtcagg
    41101 atttctgtag caaaatcctc agcaaactgg gcgtaacgac atcgtggttt gatccgctga
    41161 ttggtgccga tatcgttaag catctgcagc caaacactaa aatcgtgttt ctggaatcgc
    41221 cgggttccat cactatggaa gtccacgacg ttccggcgat tgttgccgcc gtgcgcagtg
    41281 tggtgccgga tgccatcatt atgatcgaca acacctgggc agccggtgtg ctgtttaagg
    41341 cgctggattt tggcatcgat gtttctattc aggccgccac caaatatctg gttgggcatt
    41401 cagatgcgat gattggcact gccgtgtgca atgcccgttg ctgggagcag ctacgggaaa
    41461 atgcctatct gatgggccag atggtcgatg ccgataccgc ctatataacc agccgtggcc
    41521 tgcgcacatt aggtgtgcgt ttgcgtcaac atcatgaaag cagtctgaaa gtggccgaat
    41581 ggctggcaga acatccgcaa gttgcgcgag ttaaccaccc tgccctgcct ggcagtaaag
    41641 gacacgaatt ctggaaacga gactttacag gcagcagcgg gctattttcc tttgtgctta
    41701 agaaaaaact caatgatgaa gagctggcga actatctgga taacttcagt ttattcagca
    41761 tggcctactc gtggggcggg tatgaatcgt tgatcctggc aaatcaacca gaacatatcg
    41821 ccgccattcg cccacaaggc gagatcgatt ttagcgggac cttgattcgc ctgcatattg
    41881 gtctggaaga tgtcgacgat ctgattgccg atctggacgc cggttttgca cggattgtat
    41941 aacattgcca cttttggaca attttgcaga cattttattg tgaaaagtct taaattgttg
    42001 cgtccgggat caaggcgtcc cggacgattc aggagtacaa taggcagata aaggcttaaa
    42061 cgctgttcca caggaaagtc catggctgtt attcaagata tcatcgctgc gctctggcaa
    42121 cacgactttg ccgcgctggc ggatcctcat attgttagcg ttgtttactt tgtcatgttt
    42181 gccacgctgt ttttagaaaa cggcctgctg cccgcctcat ttttgccagg cgacagcttg
    42241 ttgatactgg caggcgcatt gattgcccaa ggggttatgg attttctgcc tacgattgcg
    42301 attctgaccg ccgcagcaag tctgggctgc tggctaagtt atattcaggg gagctggtta
    42361 gggaatacca aaacggtgaa aggctggctg gcacagcttc ctgctaaata tcaccagcgc
    42421 gccacctgca tgtttgaccg ccacggtctg ctggcgctgc tggctggacg ttttcttgca
    42481 tttgtccgta cgctgctgcc aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc
    42541 cagtttttca actggttaag tggattgctg tgggtcagcg tggtaaccag ttttggctat
    42601 gccttaagta tgattccgtt cgttaaacgc catgaagatc aggtaatgac gttcctgatg
    42661 atcctgccaa ttgccttgtt aaccgctggc ttgttaggca cgctgtttgt ggtgattaaa
    42721 aaaaaatact gtaacgcctg acgattttcc ccgttcccgg ctgctgtacc gggaacgtat
    42781 ttaattcccc tgcatcgccc gcattcttgc cgcatcttcc cccggcgtca caccgaagta
    42841 acgtttaaac tcacggctaa attgcgatgc gctttcatag ccgacgcgca tcgctgctgc
    42901 gctggccttc atgccgtcat ggatgatcat catccgcgcc ttatgcagac ggtaattctt
    42961 caaatactgc aacggcgagg tgctggtgac agacttaaaa ttatggtgga acgccgatac
    43021 gctcatgttg gcttctgccg ccagttgctc gacgctcagg ttttcggtgt atttattctc
    43081 aattcgtttc agcacgcggc taatcagact gaagtgagtc tggcgactga ccagcgccag
    43141 taacgcgccg ccgcaaggtc cggtcagcac gtagtacaga atttcgcgga tgatctgttt
    43201 gccgagaata cgcgcatcca gtggtcgctc catcacgtcg agtaaccgct ccgccgcgca
    43261 taaaatctct tctgataacg tggcggagtt aatcccgctg gctgccatcg acggctggaa
    43321 atgctcatct tcgccaatgt ccatcaacag ttcctgtaac tgcaaaatat cgacattgag
    43381 acgcaaccct gccagcggca cctctgacgt ggcataggtt tcgcactcaa acggcaacgg
    43441 caccgtcagc agcaggtatt cattggcatc ataacgaaac acgcgttcat tgatataacc
    43501 gattttatgc ccggaaaaga gaattatgat gccaggctcg tacatcaccg gtgtacgtgc
    43561 gaaaggcgtc tcgccataca acaaacgcac atcgggcaac agttctgaca aactattttc
    43621 tttatttttc agtttattaa ctttatccgc cagcaagcga caaatctctt cacgtttcat
    43681 atcgcgtaat ttcttaggaa taatgcggca atttgattgt gcgcaatttt gtagcatttc
    43741 tccagcactc tggagaaata ggcaagacat tggcagaaat gagcattgag agccagggcg
    43801 ctggcgatca caatgaaaaa catcaggcag atcgttctct gccctcatat tggcccagca
    43861 aagggagcaa gtaatgaaca actttaatct gcacacccca acccgcattc tgtttggtaa
    43921 aggcgcaatc gctggtttac gcgaacaaat tcctcacgat gctcgcgtat tgattaccta
    43981 cggtggcggc agcgtgaaaa aaaccggcgt tctcgatcaa gttctggatg ccctgaaagg
    44041 catggacgtg ctggaatttg gcggtattga gccaaacccg gcttatgaaa cgctgatgaa
    44101 cgccgtgaaa ctggttcgcg aacagaaagt gactttcctg ctggcggttg gcggcggttc
    44161 tgtactggac ggcaccaaat ttatcgccgc agcggctaac tatccggaaa atatcgatcc
    44221 gtggcacatt ctgcaaacgg gcggtaaaga gattaaaagc gccatcccga tgggctgtgt
    44281 gctgacgctg ccagcaaccg gttcagaatc caacgcaggc gcggtgatct cccgtaaaac
    44341 cacaggcgac aagcaggcgt tccattctgc acatgttcag ccggtatttg ccgtgctcga
    44401 tccggtttat acctacaccc tgccgccgcg tcaggtggct aacggcgtag tggacgcctt
    44461 tgtacacacc gtggaacagt atgttaccaa accggttgat gccaaaattc aggaccgttt
    44521 cgcagaaggc attttgctga cgctaatcga agatggtccg aaagccctga aagagccaga
    44581 aaactacgat gtgcgcgcca acgtcatgtg ggcggcgact caggcgctga acggtttgat
    44641 tggcactggc gtaccgcagg actgggcaac gcatatgctg ggccacgaac tgactgcgat
    44701 gcacggtctg gatcacgcgc aaacactggc tatcgtcctg cctgcactgt ggaatgaaaa
    44761 acgcgagacc aagcgcgcta agctgctgca atatgctgaa cgcgtctgga acatcactga
    44821 aggttccgac gatgagcgta ttgacgccgc gattgccgca acccgcaatt tctttgagca
    44881 attaggcgtg ccgacccacc tctccgacta cggtctggac ggcagctcca tcccggcttt
    44941 gctgaaaaaa ctggaagagc acggcatgac ccaactgggc gaaaatcatg acattacgtt
    45001 ggatgtcagc cgccgtatat acgaagccgc ccgctaagct ttttacgcct caaactttcg
    45061 ttttcgggca tttcgtccag acttaagttc acaacacctc accggagcct gctccggtga
    45121 gttcatataa aggaggaacg tatggctaat ccaaccgtta ttaagctaca ggatggcaat
    45181 gtcatgcccc agctgggact gggcgtctgg caagcaagta atgaggaagt aatcaccgcc
    45241 attcaaaaag cgttagaagt gggttatcgc tcgattgata ccgccgcggc ctacaagaac
    45301 gaagaaggtg tcggcaaagc cctgaaaaat gcctcagtca acagagaaga actgttcatc
    45361 accactaagc tgtggaacga cgaccacaag cgcccccgcg aagccctgct cgacagcctg
    45421 aaaaaactcc agcttgatta tatcgacctc tacttaatgc actggcccgt tcccgctatc
    45481 gaccattatg tcgaagcatg gaaaggcatg atcgaattgc aaaaagaggg attaatcaaa
    45541 agcatcggcg tgtgcaactt ccagatccat cacctgcaac gcctgattga tgaaactggc
    45601 gtgacgcctg tgataaacca gatcgaactt catccgctga tgcaacaacg ccagctacac
    45661 gcctggaacg cgacacacaa aatccagacc gaatcctgga gcccattagc gcaaggaggg
    45721 aaaggcgttt tcgatcagaa agtcattcgc gatctggcag ataaatacgg caaaaccccg
    45781 gcgcagattg ttatccgctg gcatctggat agcggcctgg tggtgatccc gaaatcggtc
    45841 acaccttcac gtattgccga aaactttgat gtctgggatt tccgtctcga caaagacgaa
    45901 ctcggcaaaa ttgcaaaact cgatcagggc aagcgtctcg gtcccgatcc tgaccagttc
    45961 ggcggctaac gtgcaaattc tcccggtggc ggtaatgttc cgctaccgga cttttcagaa
    46021 atcatttatt cccctcgcgt cccgcccgtt gttactcttc cttgttcagg aatgccaaat
    46081 ataaggacat catcatgcag agccggaagc tcttaaaaga acaactcatc tatatccggg
    46141 ataaacgcaa cggagaggtg aaaaacagat gaaaataata cttctgtttt tagcagccct
    46201 ggcaagtttt accgtacacg cgcagcctcc ttcacagacc gtagaacaaa cagtccggca
    46261 gatttatcag aactataaat cagatgccag tacgccttat ttcggtgaaa ccggagagcg
    46321 ggcgataact tcagcgcgta ttcaacaggc gcttaccctg aacgacaatc ttacactacc
    46381 gggcaatatt ggctggctgg attatgatcc ggtttgtgat tgtcaggatt ttggcgatct
    46441 ggtgttagaa agcgtagcga taacccaaac tgacgtcgat catgccgatg ccgttgtgcg
    46501 ctttcgtatc tttaaagatg ataaagaaaa gaccacacag acactgaaaa tggtggcgga
    46561 aaatggtcgt tgggtcattg atgatattgt cagcaatcat ggcagcgtct tacaagcagt
    46621 taatagcgag aatgaaaaaa cgctggccgc tttagcttca ttgcagaaag aacagccaga
    46681 atcctttgtt gctgaactct ttgaacatat tgctgattat agctggccgt ggacgtgggt
    46741 ggtttccgac tcttaccgcc aggcggttaa tgccttctat aaaaccacct tcaagacggc
    46801 caataatccc gatgaagata tgcaaataga acggcaattt atttacgaca atccgatctg
    46861 ttttggcgaa gagtcgctat tttcacgcgt tgatgaaatt cgagtcctgg agaaaaccgc
    46921 cgattccgcc cgcattcatg ttcgttttac gctgaccaat ggcaacaatg aagagcaaga
    46981 gctggtttta cagcggcgcg aaggtaagtg ggaaatcgct gattttatcc gcccgaacag
    47041 cggcagccta cttaagcaga ttgaggcaaa aaccgccgcc agattaaagc aatgagctga
    47101 attaaataac aattagccgg aacaataaat aaaagggaac actatatgaa aacgattttc
    47161 accgtgggag ctgttgttct ggcaacctgc ttgctcagtg gctgcgtcaa tgagcaaaag
    47221 gtcaatcaac tggcgagcaa tgtacaaaca ttaaatgcca aaatcgcccg gcttgagcag
    47281 gatatgaaag cactacgccc acaaatctat gctgccaaat ccgaagctaa cagagccaat
    47341 acgcgtcttg atgcacagga ctattttgat tgcctgcgct gcttgcgtat gtacgcagaa
    47401 tgataaaaaa atccccggca gcatgtcagt tgccggggat tttttttaac gtccaaccgc
    47461 cgctttaggg cgtttcttcg caccagcatt caccggacga gattgcgtag acgacgcttt
    47521 ttttgccgta gcaggcgtct gacgctgagt cgccatcggc gtatgtttag tcaacgccgg
    47581 acgggtattg cggttctggc gacgagcttc acgcatctct tcaatggttg gcgcaggcac
    47641 taagcaatcg cgacggctgc caatcagatg ctttttgccc atcgcttcca gcgcctgacg
    47701 gattaacggc cagtttgccg gatcgtggta acgcaacaac gctttatgca aacgacgctg
    47761 tttgtcgccc ttcggtacga agacgccttc gctcttataa ccaatcttcg ccagcggttt
    47821 tttcccggtg taatacatgg tggttgagtt cgccagcggc gatgggtaga agttctgtac
    47881 ctggtcgaga cggaaacgat gctttttcag ccacagcgcc aaattcacca tatcttcatc
    47941 acgcgtaccg gggtgcgcag agatgaaata cgggatcaga tactgctctt tacctgcctg
    48001 tttcgagtag gtatcgaaca gctctttaaa gcggtcatag ctgcccatgc ccggcttcat
    48061 catcttcgac aacggccctt cttcggtatg ttccggagca atcttcagat aaccgccgac
    48121 gtgatgggtc gccaactctt tgatatagcg cggatcttcc acggcgatgt cataacgtac
    48181 gcctgaggcg ataaggattt ttttaatgcc tttcagatca cgcgcacggc gatagaggtt
    48241 gatcgtcggt tcgtggttag tgtccatgtg cggacaaata tccggataaa cgcacgacaa
    48301 acggcgacaa gtttgttcag cgcgtggcga tttgcagcgc aacatataca tgttggcagt
    48361 tggcccaccg agatcggaaa tcacgcccgt aaaacctgga acggtgtcgc ggatcgcttc
    48421 gatctcatta atgatcgaat cttccgaacg gctctgaata atgcgccctt cgtgctcggt
    48481 aatagaacag aaagaacagc cgccaaagca gccgcgcata atgttgaccg aaaaacggat
    48541 catttcgtaa gccggaatac gggcattgcc ataggccgga tgtggcacgc gcttgtacgg
    48601 cagcgcaaaa acgctgtcca tctcttcggt agaaagcggg atagcaggcg ggttgatcca
    48661 cacatagcgg tcaccgtgtt tttgcatcaa tgcgcgggcg cagcctgggt tggtttcgtg
    48721 gtgcagaatg cgcgaagcat gggcgtacag cactttatcg cccttcactt tctcgaaaga
    48781 aggcagcaat acgtaggttt tttcccacgg tttctggcgc ggtggctgca cgattacagc
    48841 tttcgcttcc tgctttttcg gcgcaacagg tttgttatcc gcacacggca aatcttcgcc
    48901 atacggatgc gggattgggg cgattttccc tggggtatca agacgggtgg aatccacacc
    48961 gctccagcca ggcagcgcct cttttacgat aattgcggta ttacgcacat cgcggatttc
    49021 actaatcggc tcgcccatcg ccagacgatg cactacttcc accagcggac gttcaccgtt
    49081 accaaacatc agcatgtcgg ctttcgaatc taccagaacg gaacggcgca cggtatcgga
    49141 ccagtaatca taatgcgcgg tacggcgcag gctagcctca ataccgccga ggatcaccgg
    49201 cacatctttc cacgcctctt tacaacgctg ggtataaacc agcgtggcgc gatccggtcg
    49261 cttacccgcg acattatctg gcgtgtaggc atcatcatga cgtaaacggc gatcggcggt
    49321 atagcggttg atcatcgaat ccatgttgcc agcagtaaca ccaaaaaaca gattcggttt
    49381 acccagacgc ataaagtcgt ctttgctgct ccagtccggc tgggcgatga tcccgacgcg
    49441 aaagccctgc gcttccagca tacgaccgca aatcgccatc ccgaagcttg ggtgatcgac
    49501 atacgcgtcg ccagtaacca aaatgatgtc gcagctatcc cagccaagtt gatccatctc
    49561 ttcacgagac atcggcaaaa acggtgccgg tccaaaacag gcggcccagt actgcggcca
    49621 ggagaacagg tcgcgatccg gttggatcag ggagatagag ctcattttgc ttccagaaat
    49681 gataaaaaaa taatcaaagg ccggggatta taagccggaa cgaaagagaa atcgaaaggt
    49741 attccatact cgccctcctc gggcgagtat gaagattacg gtaccggatt gaccaacagt
    49801 tgcccaatcg agccacggtc cgccatttcc agcgtctgac tgttgaagta gaacgggaag
    49861 tgcgcccagg aaggctgacc gaaataaaca agcagctcca cttgtccgtc aacccaaacg
    49921 gtatctttcc agcctctgtc ttccgggaac ggcatcgcgc cattcacgtt acggatctgg
    49981 aacattacgc cttcaatatg gaacgcttgc ggctcgtccg cgcgtaccgt ccagcgttcc
    50041 cacgttcctt gctgcgcggt gacatcaata cggttgacgt cccacagctg tccattaata
    50101 cccgggtcat cacccagact gatatcgcga ctgcgaattg gcgaaccagc catgatttca
    50161 gttggcagca agcgcatcgg aagactgtct gtgaccagcg gcagaaggcc ggttgggcgc
    50221 agcgttagca ccaaggtaga aaccagaata ctggatggct caaagaagcc gcgaatacga
    50281 tcaacaatgc tcgccgcttc accacaggtg atcgacactt catcgccgtt gctcatatcc
    50341 accagaatct cgcggcgctc gcccggtgcc agcgaaagtt gcttcaccga tacaggagca
    50401 ggcaggaatc cctgatcgcc agaaatcaca tgtaacgggc gaccatcgct catctgtagt
    50461 tgatagcgac gagagttcga cgcgttcagc agtcgcaagc gcacccagcc acgcgagact
    50521 tcaacgtacg ggctttgtac accgttaacc agcagcgtat caccaacaaa gccgccgctt
    50581 cccggttcgt tgtattctgg cgtaccaaag ttatccagcc gtttatcctg gataatgacc
    50641 ggaaaatcat ccacaccata atggttgggg ataggcagcg acttgctgac ttcatcttcc
    50701 accagccaca ttccggcaag gccgttatag acctgctgag ccgtgcggtt gggagtattg
    50761 gcgtgatacc acagagtagc tgcgttctgg cgaataggca gtacgggtgc ccagtcagcg
    50821 tttggcgaca tcatccgtgc cggaccgccc atcagcgggc ctggtacctg tagcccggcc
    50881 accgtcattg agacattttc tgtcaggcgg ttgctgtaaa taagcttaac atcgtcgccc
    50941 ttccagacgc ggatagtcgg ccccaggtaa cgaccattga ttccccagac cgacgcgcgt
    51001 gtccctggcg taaatgacca gtgcgcacgt tgtacagtca taaacagcgg ttgcccacgg
    51061 cgagattcaa gtagcggcgg aacgggtagc ggttgctgtt gcccggctgc gctggccttc
    51121 aggggaacag cgcctgcaca aagtgcaatc cccgatgcct gaatgaactg acgccgactg
    51181 agtgacatat ttgctccatg taaaactggc taaagtaata ccgtgcagga aagtgttccc
    51241 cgcaaaaata cagattgccg acttaaactt ttccggcggc ttcgcgttct gcgacttctt
    51301 tatcgagctc ggcgattttt tgttccatta tcgaacgaca atgggcagcc agctcacgaa
    51361 cctgatcttt gccatactga ctgacgtcaa ttggcggcag catttcgaca atcaccagac
    51421 cgttgtgcag tcgattaaga ttaatcttat tcgaagttgt agagacgcac acgggaataa
    51481 tcgggacgcc cgccgcaatt gccgcgtgaa atgctccagt cttgaacggt agcaggccgc
    51541 gaccacggct gcgggttcct tccgggaaca tccagatgga aatacggcgt tttttgaaat
    51601 gattcacgac ttccgcaatg gtgccgtgag ctttagtgcg attgtttctg tcgatcaata
    51661 agttgccggt taaccagtac aactgcccga agaaggggat ccacagcaag ctctttttac
    51721 ctaccgtcac cgtcggcggt tgcacaatgt tcgatgctgt caccatgtca tagttgttct
    51781 ggtggttagc gatatagata gcattgccgt agttttcagc atctgcaggt ttgcggcact
    51841 caactttcag gccgaacagt ggcgcaagac ggccaaacat atgcccaaag gtggccacat
    51901 gtttcgggtt acgcgggctg aaaaggcagt aaatggagcc gaatacacag actaagatgc
    51961 tgtaaatcac ggtaataata agacgaaaga tatatagcat aacaccctct gaaggtctga
    52021 gagactggca ttgtacgtca cctgaaatca ggttagggca ttgttaaaag ccgctctctg
    52081 gaaaagcgta ttgttaatcg caacgcatga ataaacaacg gtttcaaggg aatttttatc
    52141 gtttccgggg gttgaatacg ctgccggatg acgacttaac gtttcatccg gcgttccttg
    52201 ctagcgggag gaaacagcgc cctccccggc atattactct tcgctatcac cgctgctggc
    52261 acggcgagga gagtcgatct caacacgatc gatacgctgc aaaccgcgca tcaacgtacc
    52321 gcggcgtcca cgttcgccag tgactttctg tagctcttcc ggacgcagtt taattttgcg
    52381 tttcccaaca tgaatggtca gcgtgctttg cggcggcaga acgtacagtt gcgccagacc
    52441 atcctcgcca cgcgcggctt ctgccgatgg aatgttgata atcttgttgc ctttgccctt
    52501 cgacagctgc ggcagatcac ttaccgggaa catcaacata cggcctgcct gagtgattgc
    52561 cagcagcata tcggaagcat cttcaatcac caccggcggc ataacatggg cattttccgg
    52621 taaggtgatc aaagccttac ccgcacggtt acgcgctacc agatcgttaa aggtgcagac
    52681 gaaaccgtaa cccgcatcgg aagccatcag cagtttctga tcgtcgcttt ccatcagcat
    52741 atggtcaacg gtcgccccag gcggcaacgt taatttgccg gtgagtggtt cgccctgacc
    52801 acgcgccgac ggcagcgtaa tcgggtcgat ggcatagcta cgaccggtgg aatcaacaaa
    52861 cactaccggt tggttactct tacctttcac cgccgctttg aagctatcac ccgctttata
    52921 attcaggccc ggcgcgtcga tatcatggcc tttagcgctg cgtacccagc ccatctgcga
    52981 cagcacaatg gtgacaggtt cagacggcag catgtcgtgc tcgctcatcg ctttcgcttc
    53041 ttcgcgttcc tgcaacggcg aacgacgatc gtcaccgtag gcttgcgcgt ctgcctgcag
    53101 ttctttcttc agcaggttat tcattttacg ctcggaagcc aaaatgccct gcaactggtc
    53161 gcgctctttt tccagctcac tctgctcacc gcgaatcttc atctcttcca gtttggcaag
    53221 atgacgcagt ttcagttcga ggatcgcttc cgcctgggtt tccgtaaggc caaaccgcga
    53281 catcagcgcc ggtttcggtt catcttcgtt acgaatgatc tcaatcactt cgtcgatgtt
    53341 gagaaacgcc accagcaaac cttccaggat atgcaggcgc ttgaggactt tctccagacg
    53401 atagttcagt cggcggcgca cggtatcgcg acggaacacc agccattcgg agaggatttc
    53461 cagcaggttt ttcaccgccg gacgaccatc cagaccgatc atattgaggt taatgcgata
    53521 gctcttttcc agatcggtgg tagcgaagag gtggttcatc acctgatcca tatccacgcg
    53581 gttggaacgc ggcacaatca ccagacgggt cgggttctcg tggtcagatt catcgcgcag
    53641 atcgtcaacc atcggcagct ttttgttgcg catttgcgca gcaatttgct ccagtacgcg
    53701 cgcacctgaa acctgatgcg gtaatgcgct gataaccacc gcgccatctt ctttcttcca
    53761 caccgcgcgc atacgcactg aaccacgtcc gttctcgtag attttacgaa tctcggcgcg
    53821 cgaagtgata atttccgctt cagtcggata atccggcccc tgcacgatat ccagcagctg
    53881 atcgagcgta gttttcggct ggtcgattaa tgcgattgcc gcctgagcta cttcacgcag
    53941 gttatgcggt ggaatatcgg tcgccatgcc gacggcaata ccggtggtgc cgttaagcaa
    54001 aatgtttggc agacgggcag gtagcatttt cggctcctgc aaagtgccgt cgaagtttgg
    54061 cacccagtca gccgtcccct gccccagctc gctcaatagc agctcggaat atttcgacaa
    54121 ccgggattcg gtgtaacgca ttgccgcgaa cgatttcgga tcgtccggcg cgccccagtt
    54181 cccctgacca tcaaccagcg gataacggta agagaacggc tgcgccatca ggaccatcgc
    54241 ttcataacag gcgctatcgc cgtgcggatg gtatttaccc agtacgtcac cgacggtacg
    54301 ggccgatttt ttaaatttgg cgctggcatt caggcccagt tcagacatcg catacacaat
    54361 gcggcgctga acaggtttca gaccatcacc aataaacggc aacgcacggt ccatgatcac
    54421 gtacatggag tagtttaagt aggcgttttc cgtaaattca tgtagcgcaa ggcgctctgc
    54481 catatcgctc attaattctg attcctcaac ttattcgcca gccttcaaag gggcaatatt
    54541 gccggagata ctacattatc tgccgcgtcg agtcacagga gatcagcgtg aatagcgccg
    54601 ccggacgtgc tccgacggcg ataaaggcat caatgtgcct tgatatacaa ctctttgctg
    54661 tagacttgat cggcatagag ggcagcacaa ggttgcgccc atgccacacc actcggcatg
    54721 ggggtccgct ccgggcgatt cgagaagttg aggttatgcg agtccaggga agcctaaagc
    54781 atcaaagtag tcgactgtaa gtatacggtt cagcccagtt tagctgtgtc tccaccagtg
    54841 ccccgcgcct cctgccataa gtgcggcttg ctcatggata tccccgagac ggcttacggc
    54901 gcaccggata ataaatttcg cgtatacatg gtattcctta ccggagaaat tataagccca
    54961 acaacaatac gcctgtgaca ttgttcgctc aaacttatcg cgagtgattt ggctcacact
    55021 ccggtaaaga ttccccatca cgccaacggc acataaacat ccgtttccag tcgctgctct
    55081 gtcacccctt cggcaagatt ggtgtagtga aacagaatcg gtgctttacg cattttctcg
    55141 ccgcttgcag gcagccagtg gcgaataatg ccccatatcg tgtgtgaaat atcgtccagc
    55201 tcgccaacgt ggcgggccac ggcataacgt ccaccggtaa gttcaccatt gctcacacca
    55261 taacgattat cgggaatcgg ttcgctaacg ctgccgcaga tatcaaagcg aaacgcttcc
    55321 ggtgcggtgg tggcaggggc gtcccaggcg acgccaaaag tctggctttg gttaacagga
    55381 gaaagccccg tctctttacg ccacatgata aatttcgcca cactgtagtt gagcaactca
    55441 gggctacagc ggtgcggcaa catcgccaca gggattgatg ggaaatcgat aatgtttaca
    55501 tccagtgtca ggtttgtcat tttgcctccc tgcgaaatta ttcccgggaa cgcgtcccgg
    55561 gagcggtaac aattacggat ttactttgcg gatctgtttg acgtcaattt caacagaatt
    55621 ccagtcttta tcgacttcac cctgaatctc aaccgtatct ttcggcgtca ccgtcacacc
    55681 gttccagcgt ttgtgatcga tatcaacatt gatagtaccg ctggcatctt taaacacgta
    55741 gagatcgtca gagatgcgtt caacgatatt gccgcgcagg gttacccagg tgtcgtcacg
    55801 cagggatttt gcgctttcta cagtcgttac gctgccgttc ggcccctgaa atcctccggc
    55861 ctgactttgc gttaccgatg ggccagaaaa accgccctgc tctgctgcca tcaccggtgc
    55921 actgcacagg gccattactg cgattactgc tgcgaatttt ttcatgttta ttactccctt
    55981 taatgtctgt ttccgagcat ttaacaagat agtccttaac aacttcttaa gggaaaaaaa
    56041 taaaatttaa tgctgtacag agcgcgttac aacacggttt actggcagca aatacggtta
    56101 tcgcagggat gaaaaaatgc gaattttact gatagaagat gacatgctga ttggtgacgg
    56161 catcaaaact ggccttagta aaatgggttt tagtgtcgac tggtttacac aaggtcgtca
    56221 gggaaaagag gcgctttata gcgcacctta tgatgcggtg atcctggatt taaccttacc
    56281 aggcatggat ggtcgcgata ttttgcgcga atggcgagag aaaggtcagc gtgagccggt
    56341 actgaacctg accgcgcgtg atgcgttagc ggaacgtgta gaagggctgc gtctgggagc
    56401 tgacgattat ctgtgtaaac cttttgcgtt gatagaagtc gccgccaagc tggaggctct
    56461 gatgcgccga accaacggcc aggccagcaa cgagctgcgc cacggtaacg tcatgctcga
    56521 ccccggcaaa cgtatcgcca cgctggctgg cgaaccctta acactgaaac caaaagaatt
    56581 tgccctgctg gaattactga tgcgtaacgc tggtcgggta ctgccgcgca aactgattga
    56641 agagaaactg tatacctggg acgaagaggt caccagtaat gccgttgaag tgcatgtgca
    56701 tcatctgcga cgcaaactcg gtagtgattt tattcgtacc gtgcatggta ttggctacac
    56761 attaggtgag aaatgaaatt tacccaacgt cttagtctgc gcgtcaggct gacgctaatc
    56821 tttttaattc tggcctcggt gacctggctg ctttccagct ttgtcgcctg gaaacaaaca
    56881 acggataacg tcgatgaatt gttcgacacc caactgatgc tgtttgccaa acggttaagt
    56941 acgctcgatc tcaacgaaat caacgcggcg gatcgcatgg cacagacgcc aaataaatta
    57001 aaacacggtc atgttgatga cgatgcgctg acctttgcca tctttaccca cgacggcaga
    57061 atggtcctta atgatggcga taacggagaa gatattccct atagctatca acgggaaggt
    57121 tttgctgacg ggcaactggt cggtgaagac gatccttggc gttttgtctg gatgacctca
    57181 cctgatggca aatatcgcat cgttgttggc caggaatggg aataccgtga agacatggcg
    57241 ctggcgattg ttgccgggca attgatcccg tggctggtcg cactgccgat tatgttaatc
    57301 atcatgatgg tactactggg tcgtgaactc gcgccgctga acaaactggc gctggcacta
    57361 cgtatgcgtg accctgactc ggaaaaacca ctaaacgcga ctggcgtacc cagcgaagtg
    57421 cggccactgg ttgagtcgct aaatcaactg ttcgcccgca cacatgcgat gatggttcga
    57481 gaacgacgct ttacctccga tgcagctcac gaactacgta gcccgttaac agcgctgaaa
    57541 gtgcaaaccg aagttgcgca gctctctgac gatgatccgc aggcgcggaa aaaagcactg
    57601 ctccaattac attccgggat cgatcgcgct actcgtctgg ttgatcaact gctcacgcta
    57661 tcgcggctgg actcactgga taaccttcag gacgtcgcgg agatcccgct tgaagatctc
    57721 ctacaatcgt cggtgatgga tatttaccac acggcgcagc aggcgaaaat tgacgtgcga
    57781 ctgacactca atgcccacgg catcaaacgc accggacaac cgctattgct aagtttgttg
    57841 gtgcgaaatt tgctggataa cggccccggt gtgacaccag aggcactggc gcgaattggc
    57901 gaacgcttct atcgcccacc cggacaaacc gctaccggca gtggacttgg gctatcgatt
    57961 gtccagcgaa tcgccaagct gcatgacatg aatgttgagt ttgggaatgc ggaacaaggt
    58021 ggatttgagg cgaaggtaag ctggtaactt ctctgtctat gagagccgtt aaaacgactc
    58081 tcatagattt tactaatagc aaaatataaa ccgtccccaa aaaagccacc aaccacaaac
    58141 cagacaaata acgagcgaga atacatatag ccaaaactcg aacatttccc gcataaagag
    58201 ttttcttaag ataagaataa taagtggcgt aagaagaaaa aatgctgcaa tcattgcttg
    58261 caatatacga tcttcgcctg aaacggaagg tatactgcaa atattgcctc ccgactcaag
    58321 catccagtca tacttattga aagcaaaaaa agaatctatt aaccaataag taagcattat
    58381 atatagcaat gcttcaaata ttgttttaat tttctgcttt aacatatatc agtacgctca
    58441 taaaaatcca tttataatta tcaacatgac acgttgatat tttcatcttt tacttattca
    58501 aagccatatt ttcaatcccc aaaaaaaccg aaccaaacgt gtaaattatt ccggtgcgag
    58561 taagaattat gaggaatggc tatcagtatt gtcattttca gaaaatattt atcctgcatc
    58621 ggtgagtcag agctagatca gacttttgct aaattcgcaa aagactttgc acattttgct
    58681 aatttcacag taccgctctg tgacgtacta tagtcgacaa acgtctcacc ttgagattaa
    58741 aaaatgagca acatcctgat tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa
    58801 ctgaacgaca ccctgaccga agtcgcggat ggcacactgc gcgacctcgg gcatgatgtc
    58861 cgcatcgttc gcgccgacag cgactacaat gtcaaagcgg aagtacaaaa ctttctctgg
    58921 gctgatgtgg tgatctggca gatgccaggc tggtggatgg gcgcgccgtg gacagtgaaa
    58981 aaatatattg atgatgtatt caccgaaggt cacgggactc tgtatgccag cgatggtcgt
    59041 acccgcaaag atccgtcgaa aaaatacggt tccggcagcc tggtacaggg caaaaaatat
    59101 atgctttctc taacctggaa cgcaccaatg gaagccttca ccgaaaaaga tcagttcttc
    59161 cacggcgttg gcgttgacgg tgtgtatctg ccgttccata aagcaaacca attcctcggt
    59221 atggaaccgc tgccgacatt tatcgctaat gacgtgataa aaatgcctga tgttccccgc
    59281 tatactgaag aatatcgcaa gcatcttgtg gaaatttttg gttaactaga gctcaggctt
    59341 tagaaggagt taaccatgct taccgtaatc gcagaaatcc gtactcgtcc tggtcaacat
    59401 caccgtcagg cggtattgga tcagtttgct aaaatcgttc caaccgtact gaaagaagaa
    59461 ggttgccacg gctatgcgcc aatggtggat tgcgctgctg gcgtgagttt ccagtctatg
    59521 gcaccggatt ctatcgtgat gattgagcag tgggaaagca tcgcgcacct tgaagcgcat
    59581 ctgcaaaccc cgcacatgaa ggcgtacagc gaagccgtaa aaggcgacgt gctggagatg
    59641 aatatccgta ttctgcagcc agggatttaa tcctgccttg tttgcccggc catcctgacc
    59701 gggcaatgtt ctttccttta aacctcaatc tccgccatgt cgcctttctc ttgcaaccag
    59761 ttgcggcgat cttccgagcg tttcttcgcc agcagcatat ccatcatcgc gtcagtacgc
    59821 tgatcgtctt catcatcgat agtcaactgc accagacggc gagtgttcgg atcaagcgtg
    59881 gtttcacgca attgcatcgg gttcatttcc cccagacctt taaaacgctg gacgttcggc
    59941 ttgcctttct tgcgttttaa ttgctcaagt acgccctctt tctcttcttc cgtcagcgcg
    60001 taataaacct ctttcccgag atcaatacgg tagagcggtg gcagtgcgac gtaaacgtga
    60061 ccgtgtttca ccaacgcgcg gaagtgtttt acgaacaaag cgcagagcag cgtggcaatg
    60121 tgcagaccat cagagtccgc atccgcgagg atacagattt tgccataacg aagctggctc
    60181 agatcgtcgc tgtcaggatc gataccgatc gctaccgaaa tatcatgcac ttcctgcgag
    60241 gccagcactt cgtcggaaga aacttcccag gtgttaagga tcttaccttt cagcggcatg
    60301 atcgcctgat attcacgatc gcgcgcctgc ttggcagatc cgcctgcgga gtcaccttcc
    60361 actaggaaca gttcggtacg gttaaggccc tgcgcggtac aatcagccag tttgccaggc
    60421 agcgccgggc cgctggtcag ctttttacgc accacttttt tagccgcacg catacgacgc
    60481 tgggcgctgg aaatcgccat ctctgccagc aactccgccg cctgaacgtt ctggttcagc
    60541 cacaggatga aggcatcttt caccacgccc gaaacgaatg ccgcacactg acgcgaagag
    60601 agacgctctt tggtctgccc ggcaaactgc ggatcctgca ttttaaccga cagaacatag
    60661 gcgcagcgat cccagatatc ttccgccgac agcttcacgc cgcgcggcag aatgttgcgg
    60721 tattcacaga actcacgcat cgcgtccaac aggccctgac gcagaccatt aacatgggta
    60781 ccgccctgca tcgttgggat aaggttgacg tagctttcgg tcagcagttc accgccttcc
    60841 ggcagccaca gtagcgccca gtccacagct tcagtatcgc cagcgaaatt accgataaac
    60901 ggtttttctg gcagcgtcgg taaaccgttt accgcttccg ccaggtaatc attcagaccg
    60961 tcctgatagc accagcgctg ttcggtgttg ttgatctcat ctttaaaggt aatctcaacg
    61021 ccggggcaca gtactgcttt ggccttcagc acatgcgtca ggcgtgaaac agaaaaacgc
    61081 gggctgtcaa agaaggtttc atccggccag aagtgcacgc tggtaccggt attgcgttta
    61141 ccgcaagtgc cgacaacctg taaatcctgc accttttcgc cattttcaaa ggcgatgtta
    61201 taaacctgac catcgcggcg cacgttaact tctacgcgct tcgacaaggc gttaactacc
    61261 gaaatcccca cgccatgcag gccgccagag aactggtaat ttttgttaga gaatttaccg
    61321 cctgcatgca gacggcaaag aatcagttca accgccggta ctccttcttc cgggtgaata
    61381 tccaccggca tcccgcgccc atcgtcaatc acttctaacg actggtcagc atgtaagatt
    61441 acatccacgc gttttgcgtg acccgccagt gcttcatcga cactgttatc aataacttct
    61501 tgccccaaat ggttagggcg agtggtatcg gtatacatcc ccggacggcg gcgaaccggc
    61561 tcaagcccgg tgagtacctc aatggcatca gcgttataag tttgcgtcat ggtttaagtt
    61621 agcaattcga gttgatcgtc agagatggtg cagaccaaga aaatcgacga tcgggttgaa
    61681 ataatcttcg aagcccgtga atgcgtggtt gccgccttct atgacagtct ggcggcagga
    61741 agcgtagtac gccaccgcct ggcggtaatc cagcacttca tctcccgtct gttgcagcag
    61801 ccagatcaaa tccggcgctt ccagcgggtc aatctgcatg actttgagat cgtaaatatg
    61861 gcgtgactct agcacatatt gctgcccggt gtaggggttc tcgttctgac cgagatagtc
    61921 cgtcagcaat tcaaacgggc gtaccgccgg gtttaccacc actgcgggca gcataaaaca
    61981 ttgcgacaac caggtggcgt aatatccccc cagtgacgaa ccgacaatac ccagcgaatc
    62041 gccgccatgt tccaggacaa tggattccag cagttctgcc gcgtcggaag gatacggcgg
    62101 caactgcgga atgatcatct caacgtcagg gtgatgttcc gccagccagt tttttaacaa
    62161 gctcgctttt gcagagcgcg gcgagctgtt gaaaccgtgt aaataaagaa gcgtagacat
    62221 cagtagcctt ctgaagcggt atcaggttgg aaacgtgtgt ccgccaggcg atgcacctcg
    62281 gtggtcagcg tgccatcagc atgtaactcg agagtacgcc agccgggcgc gatagtatcc
    62341 agcgtaaagt tggaacagtg cggcttaaac tgcacacagg tcgacggcgt tgccagcagg
    62401 cggcgaccgt tccagtcgag atccagctcc tgatgaatat gaccgcacag caagtatttg
    62461 acgtgcggaa acttcgccag cacggtatcc agttcgcccg cgttacgcag actgtgttga
    62521 tcgagccaac tacaacccgc aggtagcgga tgatgatgca gcagcagcaa cgtatggcgt
    62581 tctggcgcat cggccagttt acgttccagc cactcaagct gaaactcgct cagctcaccg
    62641 tgcggcacgc caaacacctg gctatccagc aacaggattt gccattgctc accaataaac
    62701 acccgcttcg ccggggagat acccgcatcc tgtaacgcgc tgtacatcgc gggctggaaa
    62761 tcgtggttgc ccggcagcca gacgcagggc gcacgaaaac ttgcgatgcc ttcagcgaaa
    62821 tgctgatagg ccgcagagga ttgatcctgc gctaaatcac ctgtcgcgac aatcaggtcg
    62881 aattcgtgct ggtgtggacg aatcgcctcc agcaccgcct ggtagctttc ccaggtgttt
    62941 acccctaaca gggcttcgtg cttttgtgca aacaggtgag tatcggtaat ttgtaaaatc
    63001 cttcctctgg cctcaccagc cagaggaagg gttaacaggc tttccaaatg gtgtccttag
    63061 gtttcacgac gctagtaaac cggaatcgcc atcgctccat gtgctaaaca gtatcgcaac
    63121 cagtccgcta aaaactggtt aatttgatgc ttttcgtcgc gttgatgcaa ctttttatta
    63181 ggataatcat accgcgcttt gaagcgaaaa atctgctggc ttgaacacac ttcagccacc
    63241 atcgcgtcat gatacagacg caccgtcatt gacggaaggc tccagtaact gatcgcgggc
    63301 gcagtctgtt ctattgtcac tagggtagtg tatcgggtcg attccacaat cgtcagccga
    63361 tattgtgcgt ttgccacctg atagcttaca gtttcgccgg gtgcgtcatt gcgcggtaac
    63421 aaacggcgca attgtgaaaa gttcatctcg cacaggcgca tcatttcagg aaagtcaggt
    63481 gtgtaacgct tcatttatgc ccactcattt tttaacgctt gatgatgcag ctgcagccat
    63541 tgcaaagcga tgaccgacgc tgcgttgtcg attttcccct cttctaccca ctggtatgcc
    63601 tgttcccggc ttaccacatg aacgcgaata tcttcgtttt catcagccag accatgaata
    63661 ccgcttgcgg tcgtggcgtc cacttcgccc accataattg acgaacgctc actggtgccc
    63721 cccgggcttg ccaggaaact taacaccggt ttggtccgtt tgactatcag tcccgcctct
    63781 tcaatcgctt cgcgacgggc aacatcttcc acactttcac cctcttcaat catcccggca
    63841 accatctcca gtagccaggg ggtttcgctg gtgtcgtacg cggcaatccg aatctgctca
    63901 atcagcacaa cttcatcacg cactgggtca aagggtagca agactgcggc gtgaccgcgc
    63961 tcaaaaattt cccgccgtac ctcatgactc atttgcccgt tgaatagacg atgacgaaat
    64021 ctataaagat ctaatgaaaa aaagccgcga taaagtgttt ctcgtgcaat aatttctaca
    64081 tcgtttttgc caaatgtaac gggcaggttg tctggcttaa gcattgttaa tgtcctagca
    64141 ctaatagtga attaaatgtg aatttcagcg acgtttgact gccgtttgag cagtcatatg
    64201 ttaaattgag gcacattaac gccctatggc acgtaacgcc aaccttttgc ggtagcggct
    64261 tctgctagaa tccgcaataa ttttacagtt tgatcgcgct aaatactgct tcaccacaag
    64321 gaatgcaaat gaagaaattg ctccccattc ttatcggcct gagcctttct gggttcagtt
    64381 cgttgagcca ggccgagaac ctgatgcaag tttatcagca agcacgcctt agtaacccgg
    64441 aattgcgtaa gtctgccgcc gatcgtgatg ctgcctttga aaaaattaat gaagcgcgca
    64501 gtccattact gccacagcta ggtttaggtg cagattacac ctatagcaac ggctaccgcg
    64561 acgcgaacgg catcaactcg aacgcgacca gtgcgtccct gcagttaact caatccattt
    64621 ttgatatgtc gaaatggcgt gcgttaacgc tgcaggaaaa agcagcaggg attcaggacg
    64681 tcacatatca gaccgatcag caaaccttga tcctcaacac cgcgaccgct tatttcaacg
    64741 tgttgaatgc tattgacgtt ctttcctata cacaggcaca aaaagaagcg atctaccgtc
    64801 aattagatca aaccacccaa cgttttaacg tgggcctggt agcgatcacc gacgtgcaga
    64861 acgcccgcgc gcagtacgat accgtgctgg cgaacgaagt gaccgcacgt aataaccttg
    64921 ataacgcggt agagcagctg cgccagatca ccggtaacta ctatccggaa ctggcggcgc
    64981 tgaatgtcga aaactttaaa accgacaaac cacagccggt taacgcgctg ctgaaagaag
    65041 ccgaaaaacg caacctgtcg ctgttacagg cacgcttgag ccaggacctg gagcgcgagc
    65101 aaattcgcca ggcgcaggat ggtcacttac cgactctgga tttaacggct tctaccggga
    65161 tttctgacac ctcttatagc ggttcgaaaa cccgtggtgc cgctggtacc cagtatgacg
    65221 atagcaatat gggccagaac aaagttggcc tgagcttctc gctgccgatt tatcagggcg
    65281 gaatggttaa ctcgcaggtg aaacaggcac agtacaactt tgttggtgcc agtgagcaac
    65341 tggaaagcgc acatcgtagc gtcgtgcaaa ccgtacgttc ctccttcaac aacattaatg
    65401 cttctatcag tagtattaac gcctacaaac aagccgtagt ttccgcgcaa agctcattag
    65461 acgcgatgga agcgggctac tcggtcggta cgcgtaccat tgttgatgtg ttggatgcga
    65521 ccaccacgct gtacaacgcc aagcaagagc tggcgaatgc gcgttataac tacctgatta
    65581 atcagctgaa tattaagtca gccctgggta cgttgaacga gcaggatttg ctggcactga
    65641 acaatgcgct gagcaaaccg gtttccacta atccggaaaa cgttgccccg caaacgccgg
    65701 aacagaatgc tattgctgat ggttatgcgc ctgatagccc ggcacccgtc gttcagcaaa
    65761 catccgcacg cactaccacc agtaacggtc ataacccttt ccgtaactga tgacgacgac
    65821 ggggcttcgg ccccgtctga acgtaaggca acgtaaagat acgggttatc tgccgcattc
    65881 ttcccccttc tcgcttcaat ttcgaccagc catcctctat tctgatgggt atttaccact
    65941 ggtcccggaa gacaaaaatg aaacggacaa aatccatacg ccacgcatcg ttccgcaaaa
    66001 actggagcgc acgccatctg acaccagtcg ctctcgcggt tgccactgtt tttatgctgg
    66061 ctggctgtga aaagagtgat gaaacagtgt ctctctatca aaatgctgac gactgttcag
    66121 ctgcaaaccc aggcaaaagc gccgaatgta ccaccgcgta caacaatgcg ctgaaagaag
    66181 ccgaacgtac tgcgccgaaa tacgccaccc gtgaagactg tgttgctgaa tttggtgaag
    66241 gtcagtgtca gcaagcacca gcccaggctg gcatggcacc agaaaaccag gcgcaggctc
    66301 agcaatccag cgggagtttc tggatgccgc tgatggccgg ttacatgatg gggcgtctga
    66361 tgggcggcgg cgcgggattt gcacagcagc cgctgttctc ctcgaaaaac ccggccagtc
    66421 cggcttacgg taaatatacc gacgcgacgg gtaaaaacta tggcgcagcc cagccaggcc
    66481 gcaccatgac cgtaccgaag acggcaatgg caccaaaacc ggcgaccacc actaccgtta
    66541 cccgtggcgg ttttggtgaa tctgttgcca aacaaagcac tatgcagcgt agcgcaaccg
    66601 gcacctcttc tcgttcaatg ggtggctgat accgatggaa agagtcagta ttaccgagcg
    66661 cccggactgg cgtgagaaag cccacgaata cggtttcaat tttcacacca tgtacggcga
    66721 gccgtactgg tgtgaagatg cttactacaa gttgaccctc gcccaggttg aaaagctgga
    66781 agaagtcacc gccgaactgc accagatgtg cctgaaagtg gtggaaaaag tgatcgccag
    66841 cgatgagctg atgaccaaat tccgcatacc aaaacacacc tggagttttg tgcgtcaatc
    66901 atggctgact caccagccat cgctttattc gcgtcttgat ctggcgtggg atggcactgg
    66961 cgaacctaaa cttctggaaa ataacgccga tacgccaacg tcactatacg aggcggcgtt
    67021 ctttcagtgg atctggctgg aagatcagct taacgccggt aacttgtcgg agggcagcga
    67081 ccagtttaac agtctgcaag aaaaactgat cgatcgcttc gttgagctgc gtgaacagta
    67141 tggcttccag ttgctgcatc tcacctgctg tcgcgacacg gtggaagatc gcggaaccat
    67201 tcagtatttg caggactgcg caaccgaagc tgaaatcgcc accgagttcc tctacatcga
    67261 cgagataggt ttaggcgaaa aaggccagtt cacggattta caggatcagg tgatttctaa
    67321 cctgttcaag ctttatccgt gggaatttat gctgcgcgaa atgttctcca ccaagctgga
    67381 agatgctggc gtacgctggc tggaaccggc gtggaagagc attatctcca acaaggcact
    67441 tctaccgcta ctgtgggaga tgttcccgaa tcacccgaac ctgctgcccg cttattttgc
    67501 ggaagatgat catccgcaaa tggaaaaata tgtggttaaa ccgatcttct cccgtgaagg
    67561 cgcaaacgtg tcgatcattg agaacggcaa aaccattgaa gcagcggaag gtccgtatgg
    67621 cgaagaaggg atgattgttc agcaattcca cccgttaccg aaattcggcg acagctatat
    67681 gctgattggt agctggctgg tgaacgatca acctgccgga attggcattc gtgaagaccg
    67741 tgcgttgatc acccaggata tgtctcggtt ttatccacat atttttgttg aataagccac
    67801 gataccggat ggcactcgcc atccggtaat tgttagccta tctgcaccga cagcatactt
    67861 aaactgccca tttctatacc atcgaccgga atggtaattg gctcctgccc atcccacaca
    67921 cctaacacat acaacaacgg cagatagtgc tctggcgttg ggttcgataa cgcgccacct
    67981 tcatggtcga gataattcac cagaggatgc tgctctaccg gcccttgcca cgtcagattc
    68041 gctttcacat actcattaaa cgacgtcgcc cacggatacg gtgaactatc accgtgccac
    68101 ttcactgtgc gcaggttatg caccacgtta ccgctggcga ccaacattat tccttcatca
    68161 cgcagcgccg cgagtttgcg ccccatttcg aaatgccagg cgacaggttt gctactgtcg
    68221 atactcaact gcaccatagg gatatcggcg tcaggataca tcttaatcag cacgccccac
    68281 gagccgtggt caaagcccca ggcttcttta tccagcgtca ccgggatcgg cgctaacagc
    68341 tcaaccagac gctgtgccag cgcaggcgaa cccggagcag gataatgcgt atcgtacagc
    68401 gcctgcggga agccaccaaa gtcatgaatc gtgggcggcg tctccatcgc ggtcactcct
    68461 gttccacggg taaaccagtg agccgaaacc accacaatcg cttgcgggcg tggcaatgtc
    68521 atccccaact tctgccagct gcgggtatac aaattatctt ccagcacgtt catcggacta
    68581 ccgtgaccta aaaacaatgc tggcatacgt gttgaagaca tgatgatatc cttaactaaa
    68641 ggtgtcattt tgatatcctc acaatacgct tgttcggcgg agtaagaacc cggataacaa
    68701 tgatgatgat catcagttat tttgacgatc tgcctgaagg tgaagattta taaggagttg
    68761 tcgatgtcag tacctctcat tctgaccata ctggcggggg cagccacgtt tattggcgcg
    68821 tttctcggcg ttctcggaca aaaaccctcg aaccgcttac tggcgttttc gctaggtttt
    68881 gcggcgggga tcatgttgct catctcatta atggaaatgc ttcctgccgc actagcagct
    68941 gaaggaatgt cgccggtgtt gggttatgga atgtttatct tcggtctgct tggctacttt
    69001 ggcctggacc gcatgttgcc gcatgcgcat ccgcaggatt taatgcaaaa atcggtgcag
    69061 ctgttgccaa aatcgatcaa gcgcacagcc attctgctca ctctcggcat cagtctgcat
    69121 aacttcccgg aagggattgc cacctttgtc acggcgagca gcaacctgga gctgggattt
    69181 ggcatcgcac tggccgtcgc gttgcacaat atccctgaag gtctggcagt ggcaggcccg
    69241 gtttatgcgg caacggggtc taaacgcacc gcgattctgt gggcggggat ttctggacta
    69301 gcagaaattc ttggtggtgt gctggcgtgg ttaatcctcg gtagcatgat ttccccggtg
    69361 gtcatggcgg caatcatggc ggcggttgca ggaattatgg tggcgctctc ggttgatgaa
    69421 ttaatgccgc tcgccaaaga gattgaccct aataataacc ccagctatgg cgtactatgt
    69481 ggaatgtcag tgatgggatt cagtttagtg ctgctacaaa cggcgggaat tggttaaaaa
    69541 caacatcaga agttgtccag cacatggaca acttctgcca gcttcacgaa agccttattc
    69601 taccttttcg gctgtctctt ctctcgtact gtttaagtat ttgttccgcg cttcaagcag
    69661 tgcgtctctg tcctggcgaa acgcccgaca ataacgtttc aaatgcagga aataacccac
    69721 cacaaccaga gcaatcaata caatccagta ccaggcgata aacatccttt tacccttaat
    69781 atttaacaat actaaacatt tagcgcataa atttcacata tcctttttcg gatatattca
    69841 tatggtcgca taataacgga acaattatat gattttgatc ataatgaaaa agaatgcctg
    69901 atattaatga aattttctca tagaaagaga agcggaatta acatcttgca tagcagggca
    69961 aaataaaaac cgaagccagc tacaacttcg gtttcttgat taaggcagta attaagcagc
    70021 ggttttcagc tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc
    70081 acgagcgcca tattgtgttt attggcaaac tcaatgcact ctggtgcacg cgccatcgtg
    70141 ccatcgtcat tagtcagctc acacagcaca ccagccggtt taaagcctgc cagcgtcatc
    70201 aaatcaatag tcgcttcagt atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga
    70261 agtgggaaaa cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct
    70321 gcgcgaacgg tcgtaatacg atcagcggca gaaacaccgg tagtcacacc ttcagccgct
    70381 tcaatggtca cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc
    70441 agatcaagtt gtttacggcg atcttcagtg atgcacaggc aaacaatacc gctaccgtgg
    70501 cgaatggtca gcgccatctg ctcaacggtc atggtttctg ccgggaagat catatcgcct
    70561 tcgttttcac ggtcttcatc atcaagcacc attacaccgc gtccttcacg cagcgcagcc
    70621 agtgcatttt caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg
    70681 gtaaaaaaac ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc
    70741 atatagccaa aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc
    70801 ggactctaac cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca
    70861 aagcgctcgc gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc
    70921 tgagaataag cggattcact ataacgctaa tgattagcgg cagcaacgca tagcttcaca
    70981 taattctggt ttatgactta cccttatcgc actacaatgg tactcaacac ctatcagtac
    71041 agggaaacta caatgattga cccgaaaaaa attgagcaaa tcgctcgcca ggttcacgaa
    71101 tcaatgccta aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc
    71161 ctacaagctc agctgacgcg cctcgacctt gtaagccgtg aagagttcga cgtccaaacg
    71221 caagtcctgt tacggactcg tgagaaactg gcgctgcttg aacaaagaat cagcgaactg
    71281 gaaaatcgca gtactgagat caaaaagcag ccagatccag agactctccc cccaacgctg
    71341 taaacatttg tctttaatgc actgtaaatt atgaattaat cctctaattt acagtgtttt
    71401 ctattacatt atcgcttaat cccattcaga gtatcatcac aaaaatgaat gattgcaatt
    71461 gaaaatataa ttgttatgta ttctttaagt tttacttaat cgatatttag actggccc
//
LOCUS       DAJVRP010000005        70707 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVRP010000005 DAJVRP010000000
VERSION     DAJVRP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.005"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..795
                     /locus_tag="OQN12_000307"
     CDS             <1..795
                     /locus_tag="OQN12_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1652778.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="OQN12_000308"
     CDS             806..1897
                     /locus_tag="OQN12_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1652779.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OQN12_000309"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="OQN12_000309"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1652780.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="OQN12_000310"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="OQN12_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1652781.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="OQN12_000311"
     CDS             4859..5254
                     /locus_tag="OQN12_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1652782.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="OQN12_000312"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="OQN12_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1652783.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="OQN12_000313"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="OQN12_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1652784.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="OQN12_000314"
     CDS             complement(6563..7603)
                     /locus_tag="OQN12_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1652785.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="OQN12_000315"
     CDS             complement(7676..8311)
                     /locus_tag="OQN12_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1652786.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="OQN12_000316"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="OQN12_000316"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1652787.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="OQN12_000317"
     CDS             complement(8937..9380)
                     /locus_tag="OQN12_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1652788.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="OQN12_000318"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="OQN12_000318"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1652789.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="OQN12_000319"
     CDS             complement(9720..10223)
                     /locus_tag="OQN12_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1652790.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="OQN12_000320"
     CDS             complement(10217..10741)
                     /locus_tag="OQN12_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1652791.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="OQN12_000321"
     CDS             10950..11945
                     /locus_tag="OQN12_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1652792.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     HYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="OQN12_000322"
     CDS             11954..12832
                     /locus_tag="OQN12_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1652793.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="OQN12_000323"
     CDS             12913..13920
                     /locus_tag="OQN12_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS1652794.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OQN12_000324"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="OQN12_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1652795.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OQN12_000325"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="OQN12_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1652796.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OQN12_000326"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="OQN12_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1652797.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OQN12_000327"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="OQN12_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1652798.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OQN12_000328"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="OQN12_000328"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1652799.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OQN12_000329"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="OQN12_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1652800.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OQN12_000330"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="OQN12_000330"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1652801.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OQN12_000331"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="OQN12_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1652802.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OQN12_000332"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="OQN12_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1652803.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OQN12_000333"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="OQN12_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1652804.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OQN12_000334"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="OQN12_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1652805.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="OQN12_000335"
     tRNA            complement(27654..27730)
                     /locus_tag="OQN12_000335"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="OQN12_000336"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="OQN12_000336"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1652806.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="OQN12_000337"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="OQN12_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="OQN12_000338"
     tRNA            complement(31514..31600)
                     /locus_tag="OQN12_000338"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OQN12_000339"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="OQN12_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1652807.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OQN12_000340"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="OQN12_000340"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1652808.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OQN12_000341"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="OQN12_000341"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1652809.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OQN12_000342"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="OQN12_000342"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1652810.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OQN12_000343"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="OQN12_000343"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1652811.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="OQN12_000344"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="OQN12_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1652812.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OQN12_000345"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="OQN12_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1652813.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="OQN12_000346"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="OQN12_000346"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1652814.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OQN12_000347"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="OQN12_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1652815.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="OQN12_000348"
     CDS             complement(41212..42177)
                     /locus_tag="OQN12_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1652816.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OQN12_000349"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="OQN12_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1652817.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OQN12_000350"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="OQN12_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1652818.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="OQN12_000351"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="OQN12_000351"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1652819.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="OQN12_000352"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="OQN12_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1652820.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OQN12_000353"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="OQN12_000353"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1652821.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OQN12_000354"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="OQN12_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1652822.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OQN12_000355"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="OQN12_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1652823.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OQN12_000356"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="OQN12_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1652824.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OQN12_000357"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="OQN12_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1652825.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OQN12_000358"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="OQN12_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1652826.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OQN12_000359"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="OQN12_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1652827.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="OQN12_000360"
     CDS             49715..50680
                     /locus_tag="OQN12_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1652828.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="OQN12_000361"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="OQN12_000361"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1652829.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="OQN12_000362"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="OQN12_000362"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1652830.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="OQN12_000363"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="OQN12_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1652831.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="OQN12_000364"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="OQN12_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1652832.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="OQN12_000365"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="OQN12_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1652833.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="OQN12_000366"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="OQN12_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1652834.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="OQN12_000367"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="OQN12_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1652835.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="OQN12_000368"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="OQN12_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1652836.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="OQN12_000369"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="OQN12_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1652837.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="OQN12_000370"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="OQN12_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1652838.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58505
                     /gene="yrbL"
                     /locus_tag="OQN12_000371"
     CDS             57879..58505
                     /gene="yrbL"
                     /locus_tag="OQN12_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1652839.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58502..59229)
                     /gene="mtgA"
                     /locus_tag="OQN12_000372"
                     /pseudo
     CDS             complement(58502..59229)
                     /gene="mtgA"
                     /locus_tag="OQN12_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59226..59879)
                     /gene="elbB"
                     /locus_tag="OQN12_000373"
     CDS             complement(59226..59879)
                     /gene="elbB"
                     /locus_tag="OQN12_000373"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1652840.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60109..62445)
                     /gene="arcB"
                     /locus_tag="OQN12_000374"
     CDS             complement(60109..62445)
                     /gene="arcB"
                     /locus_tag="OQN12_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1652841.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62541..63470)
                     /locus_tag="OQN12_000375"
     CDS             complement(62541..63470)
                     /locus_tag="OQN12_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1652842.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64145..68605
                     /gene="gltB"
                     /locus_tag="OQN12_000376"
     CDS             64145..68605
                     /gene="gltB"
                     /locus_tag="OQN12_000376"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1652843.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68618..70036
                     /gene="gltD"
                     /locus_tag="OQN12_000377"
     CDS             68618..70036
                     /gene="gltD"
                     /locus_tag="OQN12_000377"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1652844.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70220..70699
                     /locus_tag="OQN12_000378"
     CDS             70220..70699
                     /locus_tag="OQN12_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCS1652845.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17317 a        19177 c        17550 g        16663 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccgccatta ccgtcatggt gcggtaaaag aagacgttac gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgc
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcagt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acgccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgctcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctactgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgcatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccattatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tcgtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaagctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagttaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgctc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttctgccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgggc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc actttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgataaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatct cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggtggcg ataaagagat ccgccgtgag ttaaagtact
    58021 acgcgcatct tggtcgccgg ttaaaagact ggagtggaat accgcgctat cacggtaccg
    58081 ttgagacgga ttgcggaacc ggttatgtct acgatgtgat tgccgatttt gacggcaagc
    58141 cttccattac gctgaccgaa tttgctgaac aatgccgtta cgaagaagac attgcacaac
    58201 tgcgccagct actgaaacag ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt
    58261 cgctgaagcc gcagaatatc ctctgtcacc gcatcagtga gtcagaggtt atcccggtcg
    58321 tctgcgacaa cattggtgaa agcacattga ttccactggc gacctggtcg aagtggtgtt
    58381 gtctgcgtaa gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg ctggctattg
    58441 ccctgcagaa agatctgcaa cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag
    58501 cttaatccag ctggtgttgt tgcataaacg gctcaccgcc taactgatac atctgccgta
    58561 aaatccacgc ctgacggcta cgcacgtagc ccgatggcga ggagactttg aaacgaagtg
    58621 gattaggtaa tacagctgcc agtaatgcag cttccgaccg ggtaagtttg ctcgcgggtt
    58681 tgtggaaata acgttgtgcc gcagcttcga cgccaaacac accgtcgcca aattcggcga
    58741 tattcaggta aaccgtcagg atacgctttt tgctccagac tgtttctatc cccagcgtta
    58801 atccagcttc cagccctttt cgtacccagc tacgcccatc ccataaaaag agattttggc
    58861 cgtctgctga gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc
    58921 cagggctttc tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc
    58981 tgcggcaatc accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga
    59041 atgtgccacg taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac
    59101 cgctgagaag ggaacaggcg caacgctaaa caacgcgatg cccccgcccc agaaaacggc
    59161 gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct
    59221 tttactcatt cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct
    59281 tctgcaatgt tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca
    59341 accacgatat catcgacagg acacggcaca tgctccgcgc ccatctcttc cagcacttct
    59401 gcggtatcga tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc
    59461 atcgccgggg caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc
    59521 agcgccttta attcacggtc aacggtgcat tcgctaccaa gactggcaaa attgcttaaa
    59581 ttcttcgccg cgccaacccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc
    59641 tgggccagag gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacattgcgc
    59701 gtttccgtca tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt
    59761 gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca
    59821 tgaatttcag aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt
    59881 gtgatcatcc tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca
    59941 tcacacattt aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc
    60001 ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag
    60061 atttccctgg tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt
    60121 ggcttttgcc acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc
    60181 ttcaatccat tcaccgacgt tatcttccca ggcgggaagg tcaggagact gaatttgctg
    60241 acccagctgt tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc
    60301 ttcctcaaca atgccttttt tatcctgcgc cgtcagattc aactccagca cgctgacata
    60361 gcccggcatc atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag
    60421 ttcgagatac tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc
    60481 tgtcgtcacc gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt
    60541 tagcgccgga accgaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc
    60601 ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg
    60661 ataacgcttc gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc
    60721 cagcaacacc aggtcgtatt cgcccggttt aaacatctcc agcgccgcct tgccggtcat
    60781 ggcgacatca acgctgttac ctaatttttc cagcacagaa cgcgcgacaa tcacgttcag
    60841 ttcaatgtct tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa
    60901 cgcatcatcg acctcttctg ccaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc
    60961 tttgccctgt tcactggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga
    61021 aacggccaga ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg
    61081 gtaatacatg gcgaagattt tatccagctc atcctgcgga atgccgatac cagaatcttc
    61141 cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg
    61201 ctgggtgaat ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc
    61261 gtcggtaatg acctgatgcg gtaatggcag cgtaggctcc aggttaaagc gcaatccttt
    61321 ttgttgcgcc tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac
    61381 cggctgatta tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt
    61441 aaagatattc cccagcgtga cggccgaaac atggatagtt ttgagatatt tttcctgctc
    61501 ggcggtgagt tcggtatcca gcagaatgcg gctcaggccg acgataccgt tcagcggtgt
    61561 acgcaattcg tgactgatgg tggaaataaa cgtcgttttg tcgcggctgg cgcgttcaag
    61621 cgcatcctga taccgcttac gctcggtaat gtcgcgacca aagcccatca aaccgtgacg
    61681 tttacccacg cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc
    61741 cgggtaatcc agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc
    61801 ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac
    61861 cagttgtttt tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc
    61921 tttatcttcg ttacgataaa aaaccaagtc gggtgaagca tcaaggaagg aacgtaagaa
    61981 tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag
    62041 ttggccgaag gtttcctgca gttctgcttc cgctttttca cgaacggcaa tttcctgatt
    62101 tagctgggca atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc
    62161 ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac
    62221 caccgataga aagtagaccg cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat
    62281 aacatcaatg ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat
    62341 ggcaagaacg acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat
    62401 catcaggtca acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca
    62461 cgacaacctg ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat
    62521 gcggaattcc cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat
    62581 ccctgtacgc catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt
    62641 tcgcaccaca gcggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag
    62701 atcacttccg gtggcgtatg gcgaatcatt tctccggcag tgagcgtata atcctccagt
    62761 tcaataccgt tcaaacgcac cgcttcccag gctttcgcca taatgctgcc tttcacaata
    62821 tgcagcggat gcagctttat gccgtccacg ctggtttcaa ccactcgctc cagcgtttgc
    62881 aaacattcag cctgcccttc gccaggcagg ccgacaatta agtgggaaca caccttcagc
    62941 ccgcgctgac gtgccagctg ggttgtacgc tgataacagg caaaatcatg gccgcggttg
    63001 atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca gctccagcca cacttcgtaa
    63061 ccctggtcct tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgg
    63121 gtaccaacac acaaaccgac aatattggcc tggctcaccg cctgctgata catagaacgc
    63181 agcacctgaa cttccgcaaa ggtgctggta tacgcctgaa agtaggccag atagcgttta
    63241 gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg caatggaacg atgctgctgc
    63301 gcttcatcgg caaacgaggc aacattacag aatgtgcagc caccacgccc gatggtaccg
    63361 tcacggttag ggcagctaaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa
    63421 cgacgggtga gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag
    63481 accgcgcctt gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg
    63541 gtcaatcgtc ccggcctgct tttatcaact gcataatcaa tcaaaattac cgaaatttca
    63601 tgcacaatca cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg
    63661 attttaggca aaaacagtag catgaaacgt cattaccaat taaggcagta taaaatgctg
    63721 gttttctcgt cagttcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt
    63781 gactgtttct ctaacgactc tccttttagc cttaaagata aaatctattt taatttcagc
    63841 tatttaataa agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac
    63901 ctgtatcagc tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg
    63961 ctcatcatat ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag
    64021 gacaacaagg gggcgaatgc gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct
    64081 ttctgtgccc gtgcgcagcg gttcggaagt ggggttcccg cagagcctgg gggaggttca
    64141 cgatatgttg tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca
    64201 catagaaggc gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat
    64261 gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt
    64321 acaaaaaccg gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa
    64381 aaactacgct gtcgggatgc tcttcctgaa taaagatcct gaactcgccg ctgccgcacg
    64441 ccgcatcgtt gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt
    64501 ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc tctctgccac gcattgagca
    64561 aatttttgtg aacgccccgg caggctggcg tccacgcgat atggagcgcc gtctgtttat
    64621 cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct
    64681 gtcgaatctg gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta
    64741 tctggatctt gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc
    64801 cactaacacc gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg
    64861 tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca
    64921 gacgccgctg atccctgacc tgcacgacgc cgcgccattc gtcaacgaaa ccggttctga
    64981 ctccagttcc atggataaca tgctggaact gctgctggca ggcgggatgg atatcatccg
    65041 cgccatgcgc ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact
    65101 gcgtgccttc tttgacttta actccatgca tatggagccg tgggatggtc cggcgggcat
    65161 cgtgatgtcc gatggtcgct tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc
    65221 acgctacgtc attaccaaag acaagctcat cacctgcgcc tctgaagtcg gtatctggga
    65281 ttaccagcct gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat
    65341 cgacactcgc agtgggcgta ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg
    65401 ccacccttat aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct
    65461 gcccgatgaa gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa
    65521 acagtttaac tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg
    65581 tcaggaagcg gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc
    65641 gcgcattatt tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga
    65701 cccgctgcgt gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt
    65761 cttttgcgaa gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta
    65821 ctccgatttc aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga
    65881 tatcaccttt gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tatgcgacaa
    65941 agccgaaaaa atggtacgta gcggcaccgt gttgctggtg ctctccgacc ggaatatcgc
    66001 taaagatcgc ctgccggttc cggctccgat ggcggttggc gcgatccaga cccgtctggt
    66061 cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc
    66121 gcaccacttc gccgtgttgc tgggtttcgg cgcgacggct atttatccgt accttgccta
    66181 tgaaacgctg ggccgcctgg tagacaccca cgcgattgcc aaagattatc gtaccgtgat
    66241 gctcaactac cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat
    66301 ctccaccatc gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga
    66361 tgtagtgggc ctgtgcttcc agggcgcggt cagccgcatt ggtggggcaa gctttgaaga
    66421 cttccagcag gatctgctga atctgtcgaa acgtgcctgg ctggcgcgca agccgattag
    66481 ccagggcggc ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt
    66541 ggtgcgcacg ctgcaacagg cggtacaaag cggcgagtac agcgactatc aggaatacgc
    66601 gaagctggtt aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg
    66661 tgaaaacgcg gtcaacattg ctgatgttga accggcaagc gaactgttta aacgctttga
    66721 taccgccgcg atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc
    66781 gatgaatagc atcggcggta actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta
    66841 tggcactaac aaagtgtcgc gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc
    66901 ggcgtatctg gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg
    66961 cgaaggtggt cagttgccgg gcgacaaagt cactccttac atcgccaaac tgcgctattc
    67021 ggtgcccgga gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgaaga
    67081 cttagcgcag cttattttcg acctcaagca ggttaacccg aaagcgatga tctccgttaa
    67141 gctggtgtcc gaaccaggcg ttggcaccat cgcgactggc gtggcgaaag cttatgcgga
    67201 cttgatcacc atcgcgggct atgacggcgg caccggcgca agcccgctgt cgtcagtgaa
    67261 atatgcaggc tgcccgtggg aactcggtct ggtggaaacc caacaggcgc tggttgccaa
    67321 cggcttgcgt cataaaattc gtttgcaggt tgatggcggc ctgaaaacgg gtgtcgatat
    67381 catcaaagcg gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtggc
    67441 gctcggctgt aaatatctgc gtatttgcca cctgaacaac tgcgccacgg gtgtagcaac
    67501 tcaggatgac aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgactaatta
    67561 ctttgagttt atcgcccgtg aaacccgcga gctgatggca cagctgggag taacgcgtct
    67621 ggtggatctg attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca
    67681 gcagaaactg gcgctgtcga agctgctgga gactgccgag ccacatgcag gtaagtcact
    67741 ctactgcact gaaaacaacc cgccgtttga taacggccta ctgaacgcgc agttgctgca
    67801 acaggcgaaa ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa
    67861 caccgaccgt tctgtcggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca
    67921 ggggctggca gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg
    67981 tgtgtggaac gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg
    68041 taaaggcatg gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag
    68101 ccatgaagca agcattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta
    68161 tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt
    68221 agaaggcatt ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg
    68281 taaaaccggc gttaacttcg gcgcgggcat gaccggcggc ttcgcttacg ttctcgatga
    68341 aagcggtgat ttccgcaaac gcgttaaccc ggaactggtc gaagtcttaa gcgtcgacga
    68401 tctggcgatc catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcataccgg
    68461 ttctcagcgc ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacga aattcgcgct
    68521 ggttaaaccg aagtccagtg atgtaaaagc actgctgggt caccgtagtc gtagcgcagc
    68581 agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt
    68641 tatcgacctg cagcgcgttg atccgccaaa gaaaccgctg aagatccgta aaattgagtt
    68701 tgttgaaatt tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgtct
    68761 gtcgtgcggc aacccatact gcgagtggaa atgcccggta cacaactaca tcccaaactg
    68821 gctgaagctc gccaacgagg ggcgtatttt tgaagcggca gaactgtcgc atcagaccaa
    68881 cacactgccg gaagtatgcg gacgtgtctg cccgcaagac cgtctgtgcg aaggttcctg
    68941 caccctgaac gatgagttcg gtgcggtgac catcggcaac attgagcgtt atatcaatga
    69001 taaagcgttc gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa
    69061 agtggcgatt atcggtgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa
    69121 cggcgtaaaa gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg
    69181 tattccggcc ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcacagg
    69241 catgggtatt gaattcaaac tcaataccga agtgggccgc gacgtacagc tggacgatct
    69301 gctgagtgat tacgatgccg tgttccttgg cgttgggact tatcagtcaa tgcgcggcgg
    69361 gctggaaaac gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac
    69421 caaacagtta atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa
    69481 acgcgtggta gtacttggcg gtggcgacac tgcgatggac tgcgtgcgca cgtctgtacg
    69541 ccagggggca aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc
    69601 ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc
    69661 actggggatt gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga
    69721 aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca
    69781 tatcgttccg gcagatgcgg tgatcatggc gtttggtttc cgtccacaca acatggaatg
    69841 gctggcaaaa cacagcgtcg agctggattc acaaggccgc atcatcgctc cggaaggcaa
    69901 cgacaacgct ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg
    69961 ttccgatctg gtcgttaccg ccatcgccga aggtcgcaaa gcggcagacg gcattatgaa
    70021 ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa
    70081 attctccttt ctttctggaa atcgcctcgc ataatctgaa gaaaaccaat gtaatacgct
    70141 catctggatt gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg
    70201 ccatataaag ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca
    70261 aatccacaac ggcattattc atctggtcga tagcgcccga acggaaacgg tacgtagcgt
    70321 taacgcgctg atgaccgcga cgtactggga aattggccga cggattgtcg aatttgaaca
    70381 aggtggtgag gccagagcag cttatggtgc acagttgatt gatcggctat cgaaggattt
    70441 aagtcgacgg tataaacgtg gggtctctgc aaaaaactta cgccaaatga ggctttttta
    70501 cctctttttt caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg
    70561 aattccgcag acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc
    70621 caaattgaga ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt
    70681 tgtatattca tgcaaataaa cgaagga
//
LOCUS       DAJVRP010000006        67101 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVRP010000006 DAJVRP010000000
VERSION     DAJVRP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.006"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="OQN12_000379"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="OQN12_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS1652846.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="OQN12_000380"
     CDS             complement(1150..1236)
                     /locus_tag="OQN12_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1652847.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OQN12_000381"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="OQN12_000381"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1652848.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="OQN12_000382"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="OQN12_000382"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1652849.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="OQN12_000383"
     CDS             3585..3629
                     /locus_tag="OQN12_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652850.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="OQN12_000384"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="OQN12_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1652851.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OQN12_000385"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OQN12_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1652852.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OQN12_000386"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OQN12_000386"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1652853.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OQN12_000387"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OQN12_000387"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1652854.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OQN12_000388"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OQN12_000388"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1652855.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OQN12_000389"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OQN12_000389"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1652856.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OQN12_000390"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OQN12_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCS1652857.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OQN12_000391"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OQN12_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1652858.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OQN12_000392"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OQN12_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1652859.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OQN12_000393"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OQN12_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1652860.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OQN12_000394"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OQN12_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1652861.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OQN12_000395"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OQN12_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1652862.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OQN12_000396"
     CDS             complement(16981..17745)
                     /locus_tag="OQN12_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1652863.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OQN12_000397"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OQN12_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1652864.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OQN12_000398"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OQN12_000398"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS1652865.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OQN12_000399"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OQN12_000399"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1652866.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OQN12_000400"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OQN12_000400"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1652867.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OQN12_000401"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OQN12_000401"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1652868.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OQN12_000402"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OQN12_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1652869.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OQN12_000403"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OQN12_000403"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1652870.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OQN12_000404"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OQN12_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1652871.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OQN12_000405"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OQN12_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1652872.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OQN12_000406"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OQN12_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1652873.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OQN12_000407"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OQN12_000407"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1652874.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OQN12_000408"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OQN12_000408"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1652875.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OQN12_000409"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OQN12_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1652876.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OQN12_000410"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OQN12_000410"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1652877.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OQN12_000411"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OQN12_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1652878.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OQN12_000412"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OQN12_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1652879.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OQN12_000413"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OQN12_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1652880.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OQN12_000414"
     CDS             complement(40421..41815)
                     /locus_tag="OQN12_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1652881.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OQN12_000415"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OQN12_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCS1652882.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OQN12_000416"
     CDS             complement(42158..43444)
                     /locus_tag="OQN12_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1652883.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OQN12_000417"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OQN12_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OQN12_000418"
     CDS             complement(44451..45221)
                     /locus_tag="OQN12_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCS1652884.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45693..47207
                     /gene="caiT"
                     /locus_tag="OQN12_000419"
     CDS             45693..47207
                     /gene="caiT"
                     /locus_tag="OQN12_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCS1652885.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47238..48380
                     /gene="caiA"
                     /locus_tag="OQN12_000420"
     CDS             47238..48380
                     /gene="caiA"
                     /locus_tag="OQN12_000420"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCS1652886.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48509..49726
                     /gene="caiB"
                     /locus_tag="OQN12_000421"
     CDS             48509..49726
                     /gene="caiB"
                     /locus_tag="OQN12_000421"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCS1652887.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49800..51353
                     /gene="caiC"
                     /locus_tag="OQN12_000422"
     CDS             49800..51353
                     /gene="caiC"
                     /locus_tag="OQN12_000422"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCS1652888.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51462..52247
                     /gene="caiD"
                     /locus_tag="OQN12_000423"
     CDS             51462..52247
                     /gene="caiD"
                     /locus_tag="OQN12_000423"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCS1652889.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52253..52843
                     /gene="caiE"
                     /locus_tag="OQN12_000424"
     CDS             52253..52843
                     /gene="caiE"
                     /locus_tag="OQN12_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCS1652890.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="OQN12_000425"
     CDS             complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="OQN12_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCS1652891.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53586..56807)
                     /gene="carB"
                     /locus_tag="OQN12_000426"
     CDS             complement(53586..56807)
                     /gene="carB"
                     /locus_tag="OQN12_000426"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1652892.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56825..57973)
                     /gene="carA"
                     /locus_tag="OQN12_000427"
     CDS             complement(56825..57973)
                     /gene="carA"
                     /locus_tag="OQN12_000427"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1652893.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="OQN12_000428"
     CDS             complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="OQN12_000428"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1652894.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="OQN12_000429"
     CDS             complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="OQN12_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1652895.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="OQN12_000430"
                     /gene_synonym="lytB"
     CDS             complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="OQN12_000430"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1652896.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="OQN12_000431"
     CDS             complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="OQN12_000431"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1652897.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="OQN12_000432"
     CDS             complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="OQN12_000432"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1652898.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="OQN12_000433"
     CDS             complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="OQN12_000433"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1652899.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="OQN12_000434"
     CDS             complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="OQN12_000434"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS1652900.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66187..66405)
                     /locus_tag="OQN12_000435"
     CDS             complement(66187..66405)
                     /locus_tag="OQN12_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1652901.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66508..66771
                     /gene="rpsT"
                     /locus_tag="OQN12_000436"
     CDS             66508..66771
                     /gene="rpsT"
                     /locus_tag="OQN12_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1652902.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16289 a        18260 c        17268 g        15284 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc gtatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    45361 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt ttaatattgt
    45421 tttatagatc accaatattg aaagtcagat gctatttttt tacccacaaa agaatataac
    45481 aaactgatta ataaccaaaa ttaacgaaaa acgccctcta ttcactcgaa acaaaaatgt
    45541 gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    45601 cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    45661 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    45721 tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt tggcttacag
    45781 tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    45841 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    45901 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    45961 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    46021 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag
    46081 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    46141 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    46201 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta
    46261 ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    46321 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    46381 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    46441 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    46501 tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    46561 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    46621 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc
    46681 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    46741 gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca
    46801 ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    46861 tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    46921 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    46981 tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta
    47041 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    47101 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    47161 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    47221 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    47281 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    47341 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    47401 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    47461 tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg ggcgggttca
    47521 ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    47581 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    47641 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    47701 ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    47761 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    47821 ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg
    47881 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    47941 aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg
    48001 cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc
    48061 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    48121 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    48181 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg
    48241 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc
    48301 gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg
    48361 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca
    48421 ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc ggcagtcatg
    48481 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc
    48541 gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca
    48601 aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac
    48661 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    48721 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    48781 cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    48841 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    48901 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    48961 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    49021 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    49081 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    49141 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    49201 ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga tggaactggt
    49261 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    49321 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    49381 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    49441 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    49501 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    49561 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    49621 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    49681 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    49741 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    49801 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    49861 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    49921 ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca
    49981 aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt tgctggttcg
    50041 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa
    50101 gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattttatc
    50161 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    50221 atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa aatcaacaac
    50281 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    50341 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    50401 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa
    50461 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    50521 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gcgcagaagt
    50581 accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg gtgcagccgc
    50641 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    50701 agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    50761 tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga cgctggccgt
    50821 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    50881 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    50941 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    51001 ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct
    51061 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    51121 ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg
    51181 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    51241 ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    51301 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    51361 gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca ttttttgcag
    51421 ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    51481 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg
    51541 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    51601 tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc tgggatttaa
    51661 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt tttgcgggat
    51721 taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc tacgcctttg
    51781 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    51841 tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc
    51901 cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    51961 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    52021 ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    52081 tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    52141 gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc attgaagggc
    52201 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    52261 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    52321 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    52381 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    52441 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcatgg
    52501 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtaat
    52561 tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct ttgtcaaagc
    52621 aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    52681 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    52741 cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg aggaaaatcg
    52801 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcgacc
    52861 tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc ggtatcaacg
    52921 agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta
    52981 agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac taatttttcc
    53041 cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt
    53101 cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt
    53161 atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata
    53221 tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg
    53281 gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca
    53341 caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca tattaaaatt
    53401 gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat
    53461 gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa aacggtcctc
    53521 atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccacgaca
    53581 cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg
    53641 gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta
    53701 tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg
    53761 gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct
    53821 tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg
    53881 ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg
    53941 cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag
    54001 ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac gcccatgact
    54061 tccccggtag agcgcatttc tggccctaac agcgggtcaa cgcctgggaa tttattgaac
    54121 ggcagcacca cttctttcac cgagtagtac ggcggaataa cttctttggt tacgccctgc
    54181 tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg
    54241 gctttggaga cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat caggtaaact
    54301 tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc
    54361 agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt gtacgctggc
    54421 agcgaacatg cggagtcacc ggagtgcaca cccgcctgct cgatgtgctc catgatgccg
    54481 ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca
    54541 aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag
    54601 cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac
    54661 ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca
    54721 gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt
    54781 tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg
    54841 gcttccagtg cacgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct
    54901 ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag
    54961 aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg
    55021 gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg
    55081 ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta
    55141 gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca
    55201 aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc
    55261 agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag
    55321 cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc
    55381 actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta
    55441 gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag
    55501 atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca
    55561 tccaggctca ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg cagcgctttt
    55621 tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga
    55681 gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttagta
    55741 accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc
    55801 atcagttcgt ctagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa
    55861 ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca
    55921 atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg
    55981 ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc
    56041 agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag
    56101 ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg
    56161 tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc
    56221 agaccgcggg cgcaaatttc ttcaaactct tcacggttat aagcgatacc gccgccgcta
    56281 ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc
    56341 agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc
    56401 atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca
    56461 atcatggtga caccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc
    56521 agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc
    56581 ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg
    56641 gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg
    56701 gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg
    56761 cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc
    56821 ctgattactt agcggtttta cggtactgct caattaactt gataaagtgg tcgaacaacg
    56881 gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggct
    56941 tatcgatgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca
    57001 ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca
    57061 ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt
    57121 tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata
    57181 ccggaatatc ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg cacggggccg
    57241 ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg
    57301 cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga
    57361 tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt
    57421 tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg
    57481 cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt
    57541 ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt
    57601 tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat
    57661 ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca
    57721 ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag
    57781 tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat
    57841 cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg
    57901 aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg
    57961 ctgacttaat caaaacaccc tccagagaat actcactcac tttatttgca tattaattca
    58021 cagtgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca
    58081 ttctggagat atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg
    58141 gctcatgcat gttttacagg cattaagcga caaaaacatc atctaacctg ttctggaaaa
    58201 cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg
    58261 gtcaaaattt acattaaaac aacaaaaata gaagacgaaa catgtaattt ttggcagtta
    58321 tagaaaattt aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa
    58381 ttaataaatt aaatgtgtta cttttgcatc ataataaaca ttttgtggtt acaaattatt
    58441 gagatcaagc acatctcgca tatcaaaaag accactttcc ttaccactca accacaaagc
    58501 cgatcttacc gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag
    58561 acgctcgcca atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac
    58621 ggtagcaaaa ccaatggtac ctggcacacg ttcaccggta tggccttcac gactgtagac
    58681 cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc
    58741 ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct tcaataattt cgatatcggt
    58801 gtagtcaccc atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac
    58861 gctaaaattg gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt
    58921 accggcttcg tcaaaccccg tagtgccgat caccatccct ttgccatgct gccgacaaaa
    58981 agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc
    59041 ttttatcgca tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc
    59101 accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac
    59161 gccttctaat gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc
    59221 gatggcaacg cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac
    59281 cgttttcaga gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa
    59341 ttaatgataa acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc
    59401 gataacatgt gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc
    59461 cttttacatt cagatccaat gccacctgta cattggctgg tttgcccagg caaccgtcga
    59521 tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg
    59581 gcttgagggt gaataagtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc
    59641 gcagaccgct ttgcatactg ccgctacggt agtggctaaa cagggcgtgg agcatttttc
    59701 cggtgcgatt caactccggt aaagtagcga gatagtcagg ggttaatatt gcctgattgg
    59761 tgacatccag accgcacatg acaatttcga tgccgctacg gaagacacag gcagccgctt
    59821 ctggatcggc agcgatatta aactcggcat ttggtgtaca gttgccgcga ccagcagaac
    59881 cgcccatgat caccagacga cgaatatacg gtttacattc cgggcattgt aaaagtaaca
    59941 gcgcaatatt ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca
    60001 gggcatcccg aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa
    60061 agtcgtagcc agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta
    60121 ccagcggcac agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt
    60181 gcagggcatt gcgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt
    60241 gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg
    60301 ggtcagtatc gaggaagata ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa
    60361 gaccggcatt ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac
    60421 acgcagctct ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc
    60481 ttcaccacca cccaactgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc
    60541 cgatgcgccc gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc
    60601 tttcgcatcg tcaatcaaaa acgcgtgttt gcccatacgc tgggccagct ccgccagacg
    60661 gttggagttg gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag
    60721 ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc
    60781 gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catccaccga
    60841 cagcgtggtt tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac
    60901 atcgtctggc gattcgacca gatacattcc cccttcgggg ttgctgtact gccccatcgt
    60961 cccttccact tccgggtgcc cggcgtggcc gatgagaata gattcttcgc cacggcgact
    61021 ggcgcgggca acttccatat gcactttggt caccagcgga caggtggcat caaacaccgt
    61081 caaatcgcgg cttttcgctt cgttacgaac cgcctgagaa acaccgtgtg cggagaaaat
    61141 caggatcgcg ccgtccggca cttcgctaat ctgctcaata aagatagccc cacgctcgcg
    61201 caagctatcg accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc
    61261 gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc
    61321 acgcgggttg gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca
    61381 cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg
    61441 agtcgccgtt aatttcgcgg atcacaccag gcatctcact gccatccatt gcggtaaaaa
    61501 gcatgattgc gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact
    61561 gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaggtggttt
    61621 tgtcgcccac tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcgc
    61681 ccaggcggaa cagcgccggt ttaccgttgt tgcgagtaga ctcggcggtg gtgccatcgt
    61741 cgagttttag cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc
    61801 aggtttgctc ttatttttga ggatttttgc cggatgcggc ctacgatttc gtagcaatct
    61861 gtaggccgca tccggcaggg tttattattg ttttttcgct ttggaaggta aaaacccttc
    61921 cagcacgatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
    61981 gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
    62041 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
    62101 cgtggccttc gagcgataca tcatcactgc caggagcacg ctaataccaa tcgcaatacc
    62161 ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata
    62221 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
    62281 gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca
    62341 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
    62401 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg
    62461 ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac
    62521 gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc gtcgttatag
    62581 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
    62641 agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccaccc
    62701 actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
    62761 agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa caggccttcg
    62821 taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc ccacacttca
    62881 tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc gatgtgatac
    62941 agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta ctgacggtct
    63001 ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt
    63061 accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc
    63121 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
    63181 gctggatcaa aaccattcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
    63241 tagctatcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg
    63301 gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc
    63361 gaaacggtgt tgccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg
    63421 tgggtcagta cctgacgata cggcgctttg cctttcatcg cggtggagat cattagggaa
    63481 gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg
    63541 gcaaattccg gacgcacgtc aacaacagaa gagtgggtag atccggagtc aaaccatacg
    63601 tcgagagtat ccggcacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca
    63661 tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc
    63721 agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag tgacatcggt
    63781 acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg
    63841 atacgcgcct ggccccagtc cgggagccac tgcacgcctt tgatctcttt cagtgactgc
    63901 gcacgcagac ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg gaagatgatc
    63961 ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcagcagc
    64021 gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca
    64081 tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt
    64141 tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga
    64201 acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag
    64261 cccataaacg gatgggtaaa gcgcagcagc tcaagctctg caccttttac cgtgccgaga
    64321 atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga
    64381 atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt
    64441 gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg
    64501 ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc ctggaaagcg
    64561 acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa
    64621 cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc attgccgatg
    64681 attttgccca gcgcgcggat gatgttggct tcagttttga agtccatagt cagatacggg
    64741 tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg
    64801 gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt ctcacccggc
    64861 ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc
    64921 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
    64981 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
    65041 atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc
    65101 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
    65161 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt
    65221 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
    65281 tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt
    65341 atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac
    65401 aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc
    65461 acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc
    65521 ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat
    65581 tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc
    65641 agagccagat tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat acgcacacca
    65701 ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc agctttctgt
    65761 aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc tacggcaaga
    65821 aattttacgc gcaaatgctt caccaaaaga tcgctgatga aattttgcgc ggttaacgcc
    65881 gcaaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa
    65941 cgcagttttt cccgcagccg ggtcagtctt gccggggctt tatcggtagc aaacagttcc
    66001 agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc
    66061 tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc
    66121 agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg
    66181 tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg
    66241 gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggagc aaatcacggg
    66301 aagaaactga ccgcctgctg caatttttat cgcggaaaag ctgtattcac accccgcaag
    66361 ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt
    66421 gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc
    66481 atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc
    66541 attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc
    66601 atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt
    66661 aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa
    66721 gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta atcgccaatt
    66781 tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg
    66841 atgcgtgaga gaacacactg ccggttgcca gatagtcaaa agagacatgc cgttgtacta
    66901 tctgaactct tcgtggctta agaatcgtag aaccatcggc tgatacattc tgaacaagaa
    66961 tatctactca atctccagac tcccccacaa agaatatgga tattgtgata cacattgagg
    67021 tattaaactt aagaccactc tatttttgaa ttaaattcct atatacttta accattcata
    67081 aatatctcct cattagacct t
//
LOCUS       DAJVRP010000007        65798 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVRP010000007 DAJVRP010000000
VERSION     DAJVRP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.007"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OQN12_000437"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OQN12_000437"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1652903.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OQN12_000438"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OQN12_000438"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1652904.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OQN12_000439"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OQN12_000439"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1652905.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OQN12_000440"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OQN12_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1652906.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OQN12_000441"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OQN12_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1652907.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OQN12_000442"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OQN12_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1652908.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OQN12_000443"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OQN12_000443"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1652909.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="OQN12_000444"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="OQN12_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1652910.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGGQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="OQN12_000445"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="OQN12_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1652911.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="OQN12_000446"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="OQN12_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1652912.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="OQN12_000447"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="OQN12_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1652913.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="OQN12_000448"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="OQN12_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1652914.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OQN12_000449"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OQN12_000449"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1652915.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="OQN12_000450"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="OQN12_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1652916.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="OQN12_000451"
     CDS             19304..19369
                     /locus_tag="OQN12_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1652917.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="OQN12_000452"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="OQN12_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1652918.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="OQN12_000453"
     CDS             complement(19753..20376)
                     /locus_tag="OQN12_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1652919.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OQN12_000454"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OQN12_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCS1652920.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OQN12_000455"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OQN12_000455"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1652921.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="OQN12_000456"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="OQN12_000456"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1652922.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="OQN12_000457"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="OQN12_000457"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1652923.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="OQN12_000458"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="OQN12_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="OQN12_000459"
     CDS             complement(26460..26846)
                     /locus_tag="OQN12_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1652924.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OQN12_000460"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OQN12_000460"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1652925.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OQN12_000461"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OQN12_000461"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1652926.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="OQN12_000462"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="OQN12_000462"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1652927.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="OQN12_000463"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="OQN12_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1652928.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="OQN12_000464"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="OQN12_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1652929.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="OQN12_000465"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="OQN12_000465"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1652930.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="OQN12_000466"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="OQN12_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1652931.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="OQN12_000467"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="OQN12_000467"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1652932.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="OQN12_000468"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="OQN12_000468"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1652933.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="OQN12_000469"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="OQN12_000469"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1652934.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="OQN12_000470"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="OQN12_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1652935.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="OQN12_000471"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="OQN12_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1652936.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="OQN12_000472"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="OQN12_000472"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1652937.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="OQN12_000473"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="OQN12_000473"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1652938.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="OQN12_000474"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="OQN12_000474"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1652939.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="OQN12_000475"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="OQN12_000475"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1652940.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="OQN12_000476"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="OQN12_000476"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1652941.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="OQN12_000477"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="OQN12_000477"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1652942.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="OQN12_000478"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="OQN12_000478"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1652943.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="OQN12_000479"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="OQN12_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1652944.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="OQN12_000480"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="OQN12_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1652945.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="OQN12_000481"
     CDS             53914..54303
                     /locus_tag="OQN12_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1652946.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="OQN12_000482"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="OQN12_000482"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1652947.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OQN12_000483"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OQN12_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1652948.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="OQN12_000484"
     CDS             complement(55962..56162)
                     /locus_tag="OQN12_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1652949.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="OQN12_000485"
     CDS             56328..56873
                     /locus_tag="OQN12_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1652950.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="OQN12_000486"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="OQN12_000486"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1652951.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="OQN12_000487"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="OQN12_000487"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1652952.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="OQN12_000488"
     CDS             complement(58171..58995)
                     /locus_tag="OQN12_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCS1652953.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OQN12_000489"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OQN12_000489"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1652954.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OQN12_000490"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OQN12_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1652955.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OQN12_000491"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OQN12_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1652956.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OQN12_000492"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OQN12_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1652957.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OQN12_000493"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OQN12_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1652958.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OQN12_000494"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OQN12_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1652959.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="OQN12_000495"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="OQN12_000495"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1652960.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15568 a        16296 c        18122 g        15812 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatcag gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct ggggggtcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgctcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcccgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggtgcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgccaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaccaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcagcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgtggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgatagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagattcaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttggc agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg ggagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc agctgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gtgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt cattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctatgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacatctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaacatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttgagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctacca
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagcaat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa agaggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt
    65761 taaattgaag agtttgatca tggctcagat tgaacgct
//
LOCUS       DAJVRP010000008        65615 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVRP010000008 DAJVRP010000000
VERSION     DAJVRP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.008"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OQN12_000496"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OQN12_000496"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1652961.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OQN12_000497"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OQN12_000497"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1652962.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OQN12_000498"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OQN12_000498"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1652963.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OQN12_000499"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OQN12_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1652964.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OQN12_000500"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OQN12_000500"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1652965.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OQN12_000501"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OQN12_000501"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1652966.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="OQN12_000502"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="OQN12_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1652967.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OQN12_000503"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OQN12_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1652968.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OQN12_000504"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OQN12_000504"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1652969.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OQN12_000505"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OQN12_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1652970.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OQN12_000506"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OQN12_000506"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1652971.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="OQN12_000507"
     CDS             complement(15666..16703)
                     /locus_tag="OQN12_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1652972.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="OQN12_000508"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="OQN12_000508"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1652973.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OQN12_000509"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OQN12_000509"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCS1652974.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="OQN12_000510"
     CDS             19445..19885
                     /locus_tag="OQN12_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS1652975.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OQN12_000511"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OQN12_000511"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1652976.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21602)
                     /locus_tag="OQN12_000512"
                     /pseudo
     CDS             complement(20612..21602)
                     /locus_tag="OQN12_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OQN12_000513"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OQN12_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS1652977.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OQN12_000514"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OQN12_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCS1652978.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OQN12_000515"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OQN12_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCS1652979.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OQN12_000516"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OQN12_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS1652980.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OQN12_000517"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OQN12_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS1652981.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OQN12_000518"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OQN12_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS1652982.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OQN12_000519"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OQN12_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS1652983.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OQN12_000520"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OQN12_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS1652984.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="OQN12_000521"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="OQN12_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1652985.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OQN12_000522"
     CDS             complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OQN12_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1652986.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OQN12_000523"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OQN12_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1652987.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OQN12_000524"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OQN12_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1652988.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OQN12_000525"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OQN12_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1652989.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OQN12_000526"
     CDS             complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OQN12_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1652990.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36827..37423
                     /gene="rsmD"
                     /locus_tag="OQN12_000527"
     CDS             36827..37423
                     /gene="rsmD"
                     /locus_tag="OQN12_000527"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1652991.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37413..37682
                     /locus_tag="OQN12_000528"
     CDS             37413..37682
                     /locus_tag="OQN12_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1652992.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37685..38044)
                     /locus_tag="OQN12_000529"
     CDS             complement(37685..38044)
                     /locus_tag="OQN12_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1652993.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38185..38811
                     /locus_tag="OQN12_000530"
     CDS             38185..38811
                     /locus_tag="OQN12_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1652994.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38885..41083
                     /gene="zntA"
                     /locus_tag="OQN12_000531"
     CDS             38885..41083
                     /gene="zntA"
                     /locus_tag="OQN12_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1652995.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="OQN12_000532"
     CDS             complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="OQN12_000532"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1652996.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41651..42316
                     /locus_tag="OQN12_000533"
     CDS             41651..42316
                     /locus_tag="OQN12_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1652997.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42389..42946
                     /gene="dcrB"
                     /locus_tag="OQN12_000534"
     CDS             42389..42946
                     /gene="dcrB"
                     /locus_tag="OQN12_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1652998.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42950..44167)
                     /locus_tag="OQN12_000535"
     CDS             complement(42950..44167)
                     /locus_tag="OQN12_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1652999.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44301..45350
                     /locus_tag="OQN12_000536"
     CDS             44301..45350
                     /locus_tag="OQN12_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1653000.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45405..45992
                     /gene="acpT"
                     /locus_tag="OQN12_000537"
     CDS             45405..45992
                     /gene="acpT"
                     /locus_tag="OQN12_000537"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1653001.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46103..47677
                     /gene="nikA"
                     /locus_tag="OQN12_000538"
     CDS             46103..47677
                     /gene="nikA"
                     /locus_tag="OQN12_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1653002.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47677..48621
                     /gene="nikB"
                     /locus_tag="OQN12_000539"
     CDS             47677..48621
                     /gene="nikB"
                     /locus_tag="OQN12_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1653003.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48618..49451
                     /gene="nikC"
                     /locus_tag="OQN12_000540"
     CDS             48618..49451
                     /gene="nikC"
                     /locus_tag="OQN12_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1653004.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49451..50215
                     /gene="nikD"
                     /locus_tag="OQN12_000541"
     CDS             49451..50215
                     /gene="nikD"
                     /locus_tag="OQN12_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1653005.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50212..51018
                     /gene="nikE"
                     /locus_tag="OQN12_000542"
     CDS             50212..51018
                     /gene="nikE"
                     /locus_tag="OQN12_000542"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1653006.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51024..51425
                     /gene="nikR"
                     /locus_tag="OQN12_000543"
     CDS             51024..51425
                     /gene="nikR"
                     /locus_tag="OQN12_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1653007.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51545..51904)
                     /locus_tag="OQN12_000544"
     CDS             complement(51545..51904)
                     /locus_tag="OQN12_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1653008.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51901..52176)
                     /locus_tag="OQN12_000545"
     CDS             complement(51901..52176)
                     /locus_tag="OQN12_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCS1653009.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52235..53359)
                     /locus_tag="OQN12_000546"
     CDS             complement(52235..53359)
                     /locus_tag="OQN12_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1653010.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="OQN12_000547"
     CDS             complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="OQN12_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1653011.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56091..57158)
                     /locus_tag="OQN12_000548"
     CDS             complement(56091..57158)
                     /locus_tag="OQN12_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1653012.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57524..59146)
                     /locus_tag="OQN12_000549"
     CDS             complement(57524..59146)
                     /locus_tag="OQN12_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1653013.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59408..60997)
                     /locus_tag="OQN12_000550"
     CDS             complement(59408..60997)
                     /locus_tag="OQN12_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1653014.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61382..62413
                     /locus_tag="OQN12_000551"
                     /pseudo
     CDS             61382..62413
                     /locus_tag="OQN12_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62500..62976)
                     /locus_tag="OQN12_000552"
     CDS             complement(62500..62976)
                     /locus_tag="OQN12_000552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653015.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62969..63733)
                     /locus_tag="OQN12_000553"
     CDS             complement(62969..63733)
                     /locus_tag="OQN12_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS1653016.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63979..64740
                     /locus_tag="OQN12_000554"
     CDS             63979..64740
                     /locus_tag="OQN12_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1653017.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64847..65347)
                     /locus_tag="OQN12_000555"
     CDS             complement(64847..65347)
                     /locus_tag="OQN12_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653018.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT        15938 a        17621 c        16161 g        15895 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcgc atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgca gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagcagcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcagcgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg acccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgacaca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaacg gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcaacac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag catgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg ggagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggctagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaagatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcga gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gctcttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgcgc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agcttgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcaggttaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaatagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttctgccgc ttcgacaatc tctacggttt ccgcttcagc ctgccactct
    36301 tctggcgaaa cctcttcggc gttgacgtct tccggcaacg gcagctcttc acgttcgata
    36361 gccactggct ccggcgtttc ttctacgacc ggttccggct gtgcaacaac ttccgcttca
    36421 ggctgcgctt tttcactttc aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa
    36481 gtttccgcct cggcttcagt atgcgcctgc ggctgctctt caacggcttg ttcagaagcc
    36541 ttcgcaggct cttgcgcctg aacgatttct tctacaaccg gttgttcatt ctgaacttct
    36601 gtctcttttt ccggggtctg ctctttttga ccaaagccca gccaggaaaa aaagccacgt
    36661 cttttttctt tcgccatttg cgactacact cctcgctgtt gcttcatggc acagcgttaa
    36721 cgctatgtac atagcagcta aaaaaatgat gaaatagtct atcacttaac ttaattcaca
    36781 tcaccgcctg caagtatgtg ttatctggcg gattgagcaa tttatcatga aaaaaccgaa
    36841 tcattccggc agcggccaaa tccgcattat tggcgggcag tggcgaggcc gtaaactccc
    36901 ggttcctgat agcccaggtc tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa
    36961 ctggctggct ccggtcattg ttgacgccca atgtctggat tgcttcgccg ggagcggcgc
    37021 gctggggctg gaagcgttat cgcgctacgc tgcgggggca acgttgattg agatggatcg
    37081 cgtggtttct cagcagttaa ttaagaatct ggcgacacta aaagcaggca atgcacgcgt
    37141 ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc acaccgcata atatcgtgtt
    37201 tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg ataaatttac tggaagataa
    37261 cggctggctg gctgacgaag ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc
    37321 cactgttccg gcaaactggt cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg
    37381 gctgtatcaa cacgaagcac aaggagaaag tgatgctgat taatattggt cgtttgttaa
    37441 tgctctgcgt ttggggattt ttaatcctca atctggtgca tcccttcccg cgcccgctaa
    37501 atattttcgt taacgtggcg ctgattttta ccgtgctgat gcatggtatg cagctggcgc
    37561 tattgaaatc cactttaccg aaagatggcc cgcagatgac caccgccgaa aaggtgcgga
    37621 ttttcctttt cggcgtgttt gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat
    37681 aaccttattg ttcgccaaca aagctaacaa agcgcgttcc tttatagctc agcgtacctt
    37741 tatcgcccac tgtcagggcg tggtactgct gggcgtcgag gcgaaacgtc tgctccattc
    37801 ctccgctttg cggtttgaag cttgcctcat agcgcatact ggtgcctgcc ggagtcactt
    37861 cctgctggcg cgaacggcga tcgttaatcg gtttttcccg cttgttgctc accaccacca
    37921 gcttttgctg gagcggagcc atatcattat cagctttttc ccgtcgctgt tgcataaaac
    37981 gaaacgatgc ggcgacgaca attaagccaa tgataacaat aaagaaaaga ggtggtttac
    38041 tcatctttat ccctcatcgg aaaatgcgga attaagcata ccctgccagt tatggtgttg
    38101 tcatccgtcc accctcgcca ctaaactgga agcaagaccg taggcattct gcttacgaaa
    38161 aaataacgaa ttcaaggaac taagatgctt tggtcgttta tcgctgtctg tctttccgca
    38221 tggctatctg tggatgcatc gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg
    38281 ttaacccttc ttctcctgct gttactggcc tggcaagcac cgatgttcga cgccattagc
    38341 tatctggtgc tggcgggact gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca
    38401 cgtcaacgtc tgatgtacgc catcggcgcg tttttcctct cgcacctgct gtacaccatc
    38461 tatttcgcca gtcagatgac gctctctttc ttctggcctc tgccactggt gttgctggtt
    38521 ctgggtgcgc tgctactggc gattatctgg acgcggctgg aagagtaccg ttggcctatc
    38581 tgcacgttta tcggcatgac gctggtgatg gtgtggctgg caggtgaact gtggttcttc
    38641 cgtccgaccg ctccggcgct ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac
    38701 tttgtctggc tggggagcca ctatcgccga cgcttccgtg cggataacgc gattgctgcg
    38761 gcctgctact ttgccggtca cttcctcatc gtccgctcac tgtatctctg ataaaacttg
    38821 actctggagt cgactccaga gtgtatcctt cggttaatga gaaaaaactt aaccggagga
    38881 tgccatgtcg acacctgaca atcacggcaa gaaagcccct caatttgctg cgttcaaacc
    38941 gctaaccacg gtacagaacg ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc
    39001 aacgctctct gaaaacgtct ccggcacccg ctatagctgg aaagtcagcg gcatggactg
    39061 cgccgcctgt gcgcgcaagg tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt
    39121 gcaggtgttg ttcgccaccg aaaaactggt ggttgatgcc gacaatgaca tccgtgccca
    39181 ggttgaatct gcggtgcaaa aagcgggcta ttccctgcgc gatgagcagg ccgccgacga
    39241 gccacaagca tcgcgcctga aagagaatct gccgctgatt acgcttatcg tgatgatggc
    39301 aatcagctgg ggtctggagc agttcaatca tccgttcggg caactggcgt ttatcgcgac
    39361 cacgctagtt gggctgtacc cgattgctcg tcaggcatta cggctgatca aatccggcag
    39421 ctacttcgcc attgaaacct taatgagcgt agccgctatt ggtgcactgt ttattggcgc
    39481 aacggctgaa gctgcgatgg tgttgctgct gtttttgatt ggtgaacgac tggaaggctg
    39541 ggccgccagc cgcgcgcgtc agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc
    39601 cacgcgcctg cgtaacggtg agcgggaaga ggtggcgatt aacagcctgc ggcctggcga
    39661 tgtgattgaa gtcgccgcag gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt
    39721 tgccagtttt gatgaaagcg ccctaaccgg agaatccatt ccggtggagc gcgcgacggg
    39781 cgataaagtt cctgcaggag ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc
    39841 agaaccgggt gccagcgcca ttgaccggat tctgaaactg atcgaagaag ccgaagagcg
    39901 ccgcgccccc attgagcggt ttatcgaccg tttcagccgt atttatacgc cagcgattat
    39961 ggccgtcgcc ctgctggtaa cgctagtgcc gccgctgctg tttgccgcca gctggcagga
    40021 gtggatttat aaaggtctga cgctgctgct gattggttgc ccgtgtgcgt tagttatctc
    40081 aacgcctgcg gcgataacct ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa
    40141 aggcggtgcg gcgctggaac aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg
    40201 tacgctgacc gtcggtaaac cgcgcgttac cgcgattcat ccggcaacgg gtattagtga
    40261 atctgaactg ctgacactgg cggcggcggt cgagcaaggc gcgacgcatc cactggcgca
    40321 agccatcgta cgcgaagcac aggttgctga actcgccatt cccaccgccg aatcacagcg
    40381 ggcactggtc gggtctggca ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc
    40441 tgccgggaaa catcccgctg atgcatttgc tggtttgatt aatgaactgg aaagcgccgg
    40501 gcaaacggtt gtgctggtag tacgtaatga tgacgtgctg ggtatcattg cattgcagga
    40561 taccctgcgc gccgacgcta caactgccat cagtgaactg aacgcgctgg gggttaaagg
    40621 agtgatcctc accggcgata atccacgcgc agcggcggca attgccgggg agctggggct
    40681 ggagtttaaa gcgggcctat tgccggaaga taaagtcaaa gcggtgacca agctgaatca
    40741 acatgcgccc ctggcgatgg tcggtgatgg tattaacgac gcgccagcga tgaaagctgc
    40801 cgccatcggg attgctatgg gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc
    40861 attaacccat aaccacctgc gcggcctggt gcaaatgatt gaactggcac gcgccactca
    40921 cgccaatatc cgccagaaca tcaccattgc gctgggactg aaagggatct tcctcgtcac
    40981 cacgctttta gggatgaccg ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt
    41041 gctggtgacg gcgaatgcgt taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac
    41101 attgagcgcg atcggtcccc tcgcccctct ggggagaggg ttagggggtg aggaaaaggc
    41161 ggcatcgaag ccaatcagcc cctatcaacc gcctttacga atcaaataac gataaggcag
    41221 tccatccgtc tctttcgcaa ccagttcgtg ttccataaag gtacaaaacc caggaatatc
    41281 gcgggtagtg gccggatcgt cggcgataat cagcaacgtt tcgccaggct gcatattgcg
    41341 cacggttttg cgcaccatca tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt
    41401 gtggtcaggg ctggaaaaga gatcggtcat tttcttctca tcacttaaaa aaacggcgct
    41461 agtttacgcc ctgtgagtcc gtaagcaaac caggttaacg attgcgtgaa aattaaccat
    41521 tgcattgtca acgtaaagca gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt
    41581 atgttaaggt aacagacgtg tcgtacgtat tgggttccct caccccaatg gttaatcaaa
    41641 aaggtacaat atgaacgttt tctcgcaaac tcaacgctat aaggcgttgt tctggttatc
    41701 gttatttcat cttctggtga tcacctccag taactatctg gttcagcttc ccgtctccat
    41761 ttttggtttc cataccacct ggggcgcgtt tagctttccg tttatttttc ttgctaccga
    41821 cctgaccgtg cgtatttttg gcgcaccgct ggcccgacgc attatcttcg cggtaatgat
    41881 ccctgcgtta ttaatctcct acgtcatctc gtcgctattc tatatgggtt cctggcaggg
    41941 attcggcgca ctcgcccact tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat
    42001 ggcctacgcg ctggggcaaa tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg
    42061 ccgctggtgg ctggcaccga cagcgtccac actgttcggt aacgtcagcg acacgctggc
    42121 ctttttcttc attgccttct ggcgtagccc ggatgccttt atggctgaac actggatgga
    42181 aatcgcgctg gtcgattact gtttcaaagt gttaatcagt atcgttttct tcctgccaat
    42241 gtatggcgta ttactcaata tgctgttgaa aagactggca gataaatccg aaatcaacgc
    42301 tttgcaggcg agttaaaggt tcgttatcag agttgtgata agatggatga atgagccgtt
    42361 atggccgttt atcgaaagga agaagtcaat gcgcaatctg gttaaatatg tcggaattgg
    42421 cctgctggtt atggggcttg cggcctgtga tgataaagac actaacgcta cggcgcaggg
    42481 ttcggttgcg gaaagtaacg ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag
    42541 tttctcgctg ccagcggata tgaccgacca gagcggtaag ctgggaacgc aggccaataa
    42601 catgcatgtc tggtccgacg ccaccgggca gaaagcagtc atcgtcatca tgggcgatga
    42661 tccgaaagaa gatctggcgg tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga
    42721 tccgcagctg caagtggtaa ccaataaagc cattgagctg aaaggtcaca aaatgcagca
    42781 gttagacagt attatctccg cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa
    42841 cgtgggtaat caactgctga ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc
    42901 gcaaaccacc gcagaaaaca tcattaatac gctggttatt cagtaagttt taagatgatg
    42961 aggcggcctc agggacgtgt tccggaggcc gtttttttaa tcgccacgtc agtaataacg
    43021 cgattgcgac cagtcccgcc gccgccagat aaatcaccgg tacgcccgcc cagctcatca
    43081 ccagcccagc cagtggtcca gtcacgccaa gcgataaatc cataaatacg gtgtaagttg
    43141 ccagcgccgc cccctgattt tgctgcggaa ccgcttttac cgccactaca cccaatgccg
    43201 ggaacaccag cgaaaacccg gcccccgcca gtaagacgcc gattttcgcc atccacggca
    43261 tagtcgccac gccaaccagt agcaggccga ttatctcaac gctaaagcaa atcatcgcta
    43321 cgtttaagcc accgatacgg ttaatgccgt tagggaataa caaacgcgta ccgacaaacg
    43381 cacagctaaa cagcgtcagc gcgaaagccg caccgtccca acctttagcg tcataaaaca
    43441 gcgtgataaa ggtggcgatg acgccaaatc cggcggaagc cagtgccagc gccataccgt
    43501 acagccagac gcgcccaagc accgcgcgaa acggcagcgg tttgccttta ctggctttta
    43561 ccgtcggacg cgggatcgcc aacaaaatgg ccaccagcgc cacgcccata atgattaacg
    43621 ctaacgcctg caagccgccc cagtgataaa acacgacgcc taacggcgca cccatcgcca
    43681 tcgccccgta agtgacaatg ccgttccacg aaatcacccg cccgatatgc agcgagccaa
    43741 ccactccaac gccccatagg gtcgatcccg ttccggcaaa actttgccca atcccaagga
    43801 tgacgcgccc caggcaaagt aataacaggc tgatgacagg cagactggcg gttaatcctg
    43861 ccgtcagata ccccagaccg ctcaaaaagc agccgcataa accgaagacg acaatctttt
    43921 tgggtcccag caaatcggcg taacgtccgg catgagggcg gctcagcaag gtggcgaaat
    43981 attgcaggct gataaccaat cctgcccaga aggcgctaaa gcccatcaca tcatggacat
    44041 agcccggtaa tacagcgagc ggcaacccga tggtgaggta gctggcgaag ttaaacatga
    44101 caatggagac aatgcgcaaa ttcaggcgca atccgtttag cgcgggttcg gctacgggtt
    44161 cgggcatgag gatcaccaca tttttacaac agtgtttcat ttttaccatg tgctgacgtg
    44221 aaaatcagca gtaagaatca gaatattgct ggcgtgactc ccgcgctaca cttaacacaa
    44281 aaagtcacaa ggaagcccca atggaaaccc ctcaacccga taaaacgggc atgcacattc
    44341 tgctcaagtt ggcctcgctg gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca
    44401 ttgtgcagct gttactggcg ctgttttttg ccatcgtcct caacccgctc gttacctggt
    44461 ttattcgtcg gggagtacaa cgccccgttg ccattacgat tgtagtggtg gtgatgctga
    44521 tcgcactaac cgcgctggtc ggcgtactgg cggcatcgtt taacgaattt atctctatgc
    44581 tgccgaagtt taataaggag ctgacgcgca aactttttaa attgcaggag atgttgcctt
    44641 ttcttaattt gcatatgtcg ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg
    44701 ttaccttcac cacagcgcta atgaccgggc tttccggggc aatggcgagc gtgcttttgc
    44761 tggtgatgac cgtagttttt atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt
    44821 ttgcgctgaa taacccacag attcacatcg caggattaca ccgcgcactt aaaggcgttt
    44881 cgcattatct ggcgttaaag acgctactca gtttatggac aggtgtcatc gtctggctgg
    44941 ggctggcgct aatgggcgta cagtttgcgc tgatgtgggc agtactggcg tttttgctca
    45001 actacgtgcc caatatcggc gcggtaattt ccgccgtacc gccaatgatt caggtgctgc
    45061 tgtttaatgg tgtttacgaa tgtattctgg tcggcgcatt gtttttagtg gtccatatgg
    45121 tcatcggcaa tattttagaa ccacggatga tgggccatcg cctggggatg tccaccatgg
    45181 tggtatttct ttcattgtta atctggggat ggctgctcgg cccggtaggg atgctacttt
    45241 cggtgccatt aaccagcgtg tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac
    45301 tggcgatttt actggggccg ggcagaccga aaagtcggtt accgggatga ggcgacaagt
    45361 gatacgatac gcactttcat tttccattaa acgttggccc tgatatgtat cggatagttc
    45421 tggggaaagt ttcgacctta agcgcagctc cactgccacc gggtttacgc gagcaagcac
    45481 cgcaaggtcc acgacgcgaa cgctggctgg cggggcgtgc attgctttcg cacacgcttt
    45541 ccccgctacc ggagatcatc tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc
    45601 cgctatggtt caacttaagc catagcggtg acgatatcgc tctgctgttg agcgatgaag
    45661 gcgaagtcgg ctgcgatatc gaagtgattc gcccgcgcgc caactggcgc tggctggcga
    45721 acgccgtgtt cagcctcggg gaacacgctg aaatggacgc cgtgcatcct gatcagcaac
    45781 tggaaatgtt ctggcgcatc tggacgcgca aagaagccat cgttaaacag cgtggcggca
    45841 gcgcctggca aatcgtcagc gtagatagca cctatcactc ctcgctgtca gtcagccatt
    45901 gtcagctaga aaatttaagc cttgcgatct gcacccccac cccctttacg ctcaccgccg
    45961 acagtgtgca atggatcgat tcagttaact gatccgccca cccgactgcc catctattga
    46021 tccagaacag gtaatcagta tgacgaatac ttaaaatcgt catacttatt tccgccatct
    46081 attttaatcc attggggtta ccatgctctc cacactccgc cgcactctat ttgcgctgct
    46141 ggcttgtgcg tcttttatcg tccatgccgc tgcaccagat gaaatcacca ccgcctggcc
    46201 ggtgaatgtc gggccactaa acccgcacct ttacacgcct aaccagatgt tcgcccagag
    46261 catggtttat gaaccattgg tgaaatatca ggcagacggt tcggtgatcc cgtggctggc
    46321 aaaaagctgg actcattcag aagatggtaa aacctggacc ttcaccctgc gtgatgacgt
    46381 gaaattctcc aacggtgaac cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt
    46441 gctcgataac cgtcaacgtc acgcctggct ggagctggca aaccagattg ttgatgttaa
    46501 agcactcaat aaaacagagc tgcaaattac cctgaaaagc gcctactatc ctttcctgca
    46561 agaactggcc ctgccccgtc ctttccgttt tatcgctccc tcgcagttta aaaaccatga
    46621 aaccatgaac ggaattaaag cgccgattgg caccggaccg tggattttgc aggaatcgaa
    46681 actgaatcag tacgatgtct tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat
    46741 taaaaagatc acctttaacg tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac
    46801 tggcgatatc gacctgctgt acggaaacga agggttatta ccgctcgata ccttcgcccg
    46861 ctttagccag aatccggctt accacaccca actgtcacag ccgatcgaaa ccgtgatgct
    46921 ggcgctcaat accgccaaag cccccaccaa cgagctggca gtacgtgaag ctcttaatta
    46981 cgcggtaaac aaaaaatcgc tgattgataa cgcgttatat ggcacccagc aggtcgccga
    47041 caccctgttt gccccttctg tgccttacgc taatctcggc ctgaaaccgc gccagtacga
    47101 tccgcagaaa gcgaaagagt tgctggaaaa agccggttgg acgctgcctg cgggcaaaga
    47161 catccgcgag aaaaatggtc agccgctgcg cattgaactg tcgttcatcg gcaccgatgc
    47221 gttaagcaaa tcgatggcgg aaatcattca ggctgatatg cgccagattg gcgcagatgt
    47281 ctcgctgatt ggcgaagaag agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg
    47341 catgattttc caccgcacct ggggcgcgcc atacgatcca cacgccttcc tgagttcaat
    47401 gcgcgtaccg tcgcacgctg acttccaggc acagcaagga ttagccgaca agccgctgat
    47461 tgataaagag atcggcgaag tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta
    47521 tcgcgacatt ctgacccgtc tgcatgacga ggcggtttat ctgcctatca gctacatctc
    47581 gatgatggtg gtatcaaaac cggagctggg taatatcccc tacgcgccga tcgccaccga
    47641 aattccgttc gaacagatta aaccggtgaa accttaatgt tgcgttacgt attacgccgc
    47701 tttctgctgc tgatcccgat ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc
    47761 ctcggtaccg gcgacccggc gctcgattat ctgcgtttat ctaacctgcc gccgacgccg
    47821 gagatgctgg cctctacccg caccatgctc gggctggatc agccgctgta cgtccagtac
    47881 ggtacctggt tgtggaaggc gctgcatctt gactttggta tctcattcgc cagccaacgc
    47941 ccggtactgg acgatatgct gaacttcctg cccgccacgc tggaacttgc aggtgcggcg
    48001 ctggtattaa ttctgctcac ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat
    48061 cgtttgccgg atttcgccgt gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc
    48121 tggctggcat ttttgctagt gatggcgttt tcggtgtatc tgcaatggct acccgcgatg
    48181 ggctacggca gctggcagca catcatttta cctgcggttt ccatcgcctt tatgtcgctg
    48241 gcgattaacg cgcgactgct gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc
    48301 acctgggcgc ggctacgcgg cctgaatgac aaacagaccg aacgccgcca tattctgcgc
    48361 aatgcctcgc tgccgatgat caccgccgtg gggatgcaca ttggcgaatt gattggcggg
    48421 acgatgatta tcgaaaacat ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg
    48481 atttttaacc gtgactatcc ggtgattcag tgctttacgc tgatgatggt ggtggttttt
    48541 gtggtctgta atttgattgt cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt
    48601 catgaaggag cgcacgcgtg aactttttcc tctcttcccg ctggtcggta cgcctggcgc
    48661 tgatcattat cgccctgctg gcgctgattg cgctcaccag ccagtggtgg cagccgtacg
    48721 atccacaggc aattgatttg ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg
    48781 gcaccgatca cttaggtcgc gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc
    48841 tcggttcggt aatggcctgc ctgctgctgg tgctgacatt agggctggtt attggcggca
    48901 gcgccgggtt gattggcggg cgcgttgatc aggccaccat gcgcgtcgcc gatatgttta
    48961 tgaccttccc gacctcgatt ctgtcgttct ttatggttgg cgtgctcggc accgggctga
    49021 ccaacgtaat tatcgccatc gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca
    49081 gcttggtgat ttcactacgc caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg
    49141 gccatgtgcg ggtgtttgtc gatcatctgg caggcgcggt gatcccttcg ctgctggtgc
    49201 tggcaacgct ggatatcggc catatgatgc tgcacgtcgc ggggatgtct ttccttggcc
    49261 tcggtgtgac cgcgccgacc gccgaatggg gcgtgatgat taacgacgcg cgccagtata
    49321 tctggaccca gccgctgcaa atgttctggc cggggctggc gctgtttatc agcgtgatgg
    49381 cctttaacct ggtgggtgac gcactgcgcg atcatctgga tcctcatctg gtgacggagc
    49441 acgcacacta atgccgcaac agattgaact acgtaatatc gcgctacagg ccgcgcagcc
    49501 gctggtacac ggtgtatcgt taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg
    49561 tagcggcagc gggaaatcat taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt
    49621 tcgccagacg gcgggggaaa ttttagccga tggcaaaccg gtttcgcctt acgccctgcg
    49681 cggcatcaaa attgccacca tcatgcagaa cccgcgcagc gcctttaatc cactgcacac
    49741 catgcacacc cacgcgcggg aaacctgcct ggcgttaggg aaacccgccg atgacgctac
    49801 gcttaccgct gccatagaag cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta
    49861 cccgttcgag atgagcggcg gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg
    49921 tgaatcaccg tttatcatcg ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc
    49981 gcgcatcctc gatctgctgg aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt
    50041 cacccatgat atgggcgttg tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg
    50101 taaaattgtc gaacagggcg atgtagaaac gctgtttaac gcccccaaac atacggtgac
    50161 gcgcagcctg gtttccgctc atctcgccct ctacggtatg gagctggcat catgacctta
    50221 cttaacgtct gcggcctttc ccatcactat gcccacggtg gatttagcgg aaaacatcaa
    50281 catcaggcgg tgctgaataa cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg
    50341 ctgggacgca gcggctgcgg aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct
    50401 ccctcacaag ggaatattag ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa
    50461 cgtaaagcct tccgccgtga tattcagatg gtgtttcagg actccatcag tgccgtgaat
    50521 ccgcgcaaaa ccgtgcgcga gatcctgcgt gaaccgatgc gccacctgct atcactgaaa
    50581 aaatccgaac aactggcgcg cgccagcgaa atgctgaagg cggtcgatct cgatgacagc
    50641 gttctcgaca aacgcccacc gcagttaagc ggcggccagc tccagctcgt ctgcctggca
    50701 cgcgcgctgg cggtcgaacc gaaattgctg attctggatg aagccgtttc taaccttgat
    50761 ctcgtgttac aggcgggcgt cattcgcctg ctgaaaaagc tacaacaaca gtttggcact
    50821 gcctgcctgt tcatcactca tgacttacgc ctggtggaac gcttttgcca gcgagtgatg
    50881 gtgatggaca acggacaaat cgccgaaacc caggcggtgg gagataaatt aaccttttcc
    50941 tctgacgccg gacgtgtgct acaaaacgcg gtattacccg cattccccgt gcgccgtcgc
    51001 acctcagaaa aggtttaacg ccaatgcaac gagtcaccat cacgcttgat gacgatttac
    51061 tggagacgct ggacagcctg agccagcgtc gtggttataa caaccgttcc gaagctatcc
    51121 gcgatattct gcgtagtgcc ctggcgcagg aagctaccca gcagcacggc acacaaggtt
    51181 tcgcggtgct gtcgtatgtg tatgaacacg aaaaacgcga cttagccagc cgtattgttt
    51241 ccacccagca tcatcaccac gacctctccg tcgccacgct acatgtgcac atcaaccacg
    51301 acgactgtct ggaaatcgcc gtgttgaaag gtgacatggg tgacgtgcag cattttgccg
    51361 atgacgttat cgcccagcgc ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag
    51421 attgagttat ttctaaaaca aggaaagaaa agatattaac tgaacttaat ttataaaatt
    51481 aagttcagtt gcatagatag atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta
    51541 aaatttataa atgattcaaa cgctcattca gtgtttcaat aatataggta ttaacagaga
    51601 cttgttgctg cgcagccgca ttattaaggc gttcactcag cgattctgga tagcgtaacg
    51661 taaaggtctt aatcttttct gtacgcgcat acggctcaat acccgcagct ttacaatcct
    51721 caagatactc tcgcagggaa agctctcctt ctttttgcag cccctgaata ctgtcagaga
    51781 caaaatcaca ataacctgac aagcccagga atttgccacg aaatgcattc agttctggca
    51841 cgtaggtaat aacagctggt tgcccggcaa tctccataga gtttggcgtc ttcagtttga
    51901 tcatggtttt acccctatac ttagtaacca gtcacacacg ctttctacag cgcctttatc
    51961 ggtatctggc gacggatgag gccgatggaa acacgcaacg ctcatattta gtatgaactt
    52021 gcaacgcgaa cctcttcctt tcttaatttc tccgcctaat gctttaacca gtgactctat
    52081 atcagaccac ttgatccctt gaggaacagg agttttaaat atttgatcca atgtattttt
    52141 ttgtttcttc cgtaaagcct gtaccttggt tctcatggca tgtccttatg acttcacttt
    52201 atgacatcac agtatgaagt caagtttccc ttccttacgc cattgtccca atcgtcttcc
    52261 tgaatcgcag cagtgcgatg gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact
    52321 gcggccagac gatttcgaat ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa
    52381 agtgtgtcgt cggcatggtc agcataatgt cctgcaccat ctgcggcata ctttcgcgcg
    52441 gcgtggatcc accggaaagc atttgcagcg gcagcagcac cagaatcacc agcagcccca
    52501 gttgcggcat tgaacgcgct atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca
    52561 gactgagcgc cacgcccagc ataaacagcg ggatcgagcc ttcaatcggt acgcccagta
    52621 cacctttcac catcagcacc agcgataatc ccgataccac cagcaccacc agccccatcg
    52681 accagatctt cgccatcatg atctcaaacg gcgttatcgg catcaccagt aaatgttcca
    52741 ccgtgccgtg ttcacgctcg cggatcagcg ccgatccggt caatacaatc gccagcatgg
    52801 taatgttgtt gatgatcgcc atcaccccgc caaaccacgc gggatcaagg ttcgggttaa
    52861 agcgcatccg ggtttccagc gataccaacg gttcgctgtt atcacggtag cgcgcgacaa
    52921 agctgttcac ttcaccgttg ataatattct ggatataccc attgccggta aatgcctggc
    52981 tcatgcgcgt ggcatcgacg ttcacctgaa tatccggctg gcgtccggcg aggacatcac
    53041 gctgaaaatt aggcggaata tttatcgcga aggtatagcg tccggcgtcc agtccggcat
    53101 ccatctcatc ggcggtgatc atctccggtg gcaaaaacca cggacgatag aagctgttaa
    53161 cgatccggtt cgataactgc gattgatcca tatcggcaat ggcgatcggc gcgaggttca
    53221 acgatcctgg cgtaacggtc gctgacgaat acaccgacac cgtaaacgag aagacaatca
    53281 gcgtcagcat cgctttatca ccgagcagac tgcgcaactc tttgataccc agattaaaaa
    53341 tattgcgtaa atggcgcatc atccctcctg ttttttcagc agcaggatgc ttaagcccat
    53401 caccagcggg atggctatca gcaacgggat aaaaagttgc cacaaatcag tcagatccag
    53461 cgctttcgag aacgttccgc gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc
    53521 gatccaacgt ccaagccctt ccagcgaagc taccggatcg atcatcccgg aaaactgtgt
    53581 cgccgggatc aatgtgataa tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa
    53641 ggtggagatc agcagcccca ttccagtggc aatgatgata tacagcagcg ccgccaaggt
    53701 gagcgtcagg aaactgcctt tatgcggtac gccaaacaca aacaccgaca ggccgcagag
    53761 caggaaaaag ttcagcatcc ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc
    53821 gctacgggtg gtgggggtca cgtaaaggtt gataatcgac cctagctctt tttcccgcac
    53881 cacgctaagg gcgcttagca ttgacgggat catcatcagc agaagcggga tcaccgccgg
    53941 aacaatcgct ggcaggcttt ttacgtccgg gttatagcga taacgcgtct caatgttcat
    54001 cagcccgctt tggctggcgg gtgtcgattg tcggctcgcc acatcctgaa gccaactctg
    54061 gtgcattgcc tgcacgtaac cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat
    54121 ccagacgccg agttccacag gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat
    54181 ctcaatggcc accgtgatat cgcccgcacg catccgacga tcaagctcgt cataactggt
    54241 gagcggcggc tgttcgataa agtaacggga accggagagg ttgagtgtcc acgcctggct
    54301 actgacggtc tggtcgcggt cgagcaccgc aaagcgcagg ttttccacat ccatactgat
    54361 gccgtaaccc attatcagca tcaggatcac cgttcccatc agcgccagcg tcgaacgtac
    54421 tggatcgcgt cgcagttcca gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct
    54481 aaatccctga cgcggcgcgt gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt
    54541 gctctgccct gccgcttcct gcaaataggc gataaatgcc tcttccagac tggcggctcc
    54601 gcgtttctca accagttcct gcggtgtacc gctggcaagc acttttccgg cgtgcatcag
    54661 tgagatgcgg tcgcaacgtt ccgcttcgtt cataaagtgg gtggagatga agatagtcac
    54721 tttgtcctgg cgcgagagat cgaccatcaa ctgccagaac atatccctcg ccaccggatc
    54781 gacaccagaa gtaggctcat cgaggattaa catctccggg cgatgaatca ccgccaccgc
    54841 cagcgaaagc cgctggcgaa tgccgagcgg caatgactcc ggcagaacat cttcaacgtc
    54901 gttgagctta aaacgctcgc tcatttcagc cactcgcgcg ggaatttccg cttccgggat
    54961 gtgaaacaaa cgggcatgta actccaggtt ttgccgcacg gtgagttcgt tatagagcga
    55021 aaacgcctgc gacatatagc ccacacgacg gcgggtatcg atatcttttg gatcaaccgg
    55081 ttgcccaaat agccacgcct caccttcgct ggcgggcagc agtccggtaa gcattttcat
    55141 ggtggtggat ttaccgcagc cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat
    55201 gcggaaattg acgtgatcaa cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc
    55261 ttcgatggca atctctgcgt tttcaggttg atacggtggg atcactaccg cctgatgcgc
    55321 ctggcgttgc gcttgcggta acagattaat aaatgcttct tccagcgtgg cgctttgcgt
    55381 ttgctgccgc agctcttcgg cgctgccggt tgccagcact tctccggcat tcatcgctac
    55441 cagccagtcg aagcgttcgg cctcttccat ataggcggtg gcgaccagca cgctcatatt
    55501 gctctgccgc tggcgaatac tgtcgatcag atcccagaac tgggcgcggg agagcgggtc
    55561 aacccccgtt gttggctcat caaggatcaa cagttccggg tcgtggatta acgcgcagca
    55621 cagcccaagt ttttgcttca tcccgccgga gagtttacct gccggacgat cgcgaaacgg
    55681 tgctaacccg gtgctggtca gcagctcatt aattcgcact tcccgctccg ctttatcgtg
    55741 accgaacagg cgagcgaaaa aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt
    55801 tttgcccagc ccctgcggca tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg
    55861 gtcgtgcata tcgccaccca gcaccaccac attgccctgc tcaatgacgc gggcaccgga
    55921 aatcagtgac aataaactcg acttcccgac gccatccgga ccaatcagcc cgaccatgca
    55981 acgggccgga atatcgagag tgatattgtt cagcgcaacg gtttttccat aatgctggct
    56041 cacgcccgcc agttgcgcga caggcgggac gggaaccagt tccagatgcg tcattgcggc
    56101 aacctcacca cgaggtcgtc aggccacgga agttcttcat tcacccgcac ccacgctacg
    56161 cccggtaaac cggttttgac atattccaga tgctgctgga gtaattccgg tgggatacgc
    56221 gctttgacgc ggaacatcag tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg
    56281 aactgggcga cactggcgac aaaactgatg gttgcaggaa tacgcagatc tggcgcggca
    56341 tcgaggatca gccgggcttc accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg
    56401 aagaaggtca tatagacgtc gctgagatcg accatattca gcacccgacc gcctgccgcc
    56461 agcacttccc ctggctcggc aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt
    56521 tcactgtcat cgatatctgc ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga
    56581 gtttgcgcct gaatgatatt ggtgcgtgct gcttctatag ccgctttaga agccgaaacc
    56641 tgggctttcg ctgattccag cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc
    56701 agctgttgcg cagaaatagc ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg
    56761 gcaacagagt ccagttcggc ctggcgttga ttgaccagcg actgtgcggc acgagtttca
    56821 ctttgtcgtt gctccagcaa agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt
    56881 tgcgcgatgg cttccagtcg ctgttcctgc aacacgcgag tatccatctt cgccagcact
    56941 tcgccttcgc gaacaaactg gccttctttc accagaatgg tgtcgatacg cccggcaatt
    57001 ttgctggcga tatccacttc cgtcgcttca atgcgcccat tgctgacagc aaagccttcc
    57061 ggcacacccg ccgggcgcaa cagccaccac gccacgacgg cagccaccgc cagtgccccg
    57121 acaacccacc acgccagatg gcgcttactc ttatccataa tcgacccgcc ataatccctg
    57181 taagcaaacg acgcgtgtta caactgccgc cgaacgacgt tcagcggatt cttccatccg
    57241 ggataagaag gaaacgacaa atacgaccag cgcgcagagc attgagcgcc aacggggaag
    57301 gcttagtgaa aacgggtagt aatgttgtca tcatcagcat tcctggccgt aaatgaaaca
    57361 cgcatactca taagtgtgat ctataaaact ggctgacaaa acggtatcca tcctgttgga
    57421 tcgcatttca cgcctgttca gttgcaggaa cagagcattc ctttttacgt tttatcaaca
    57481 gtctcaacca gatagtaaca ttaatattca tgttgtctat ggttcagagc tgtaacattg
    57541 tgcgccttct aatcaaaggc ataagtccat ttctgttttt ttcatgctgg taagacttcc
    57601 agggagtggt tatctctggc cctgcaccag ggttaatttg gcaattatgc atgctgaaca
    57661 ttcttccagc atgtttactg tcacgggcgt taagacctaa aactgtcccc gtttcaccta
    57721 tgcgttctct cccaccattt ccgttatgtg caacaacttg atttatacct aacatttgtg
    57781 aacatgctaa tagtgcttca agtttaggtc tgtaatctgt cgaaacagaa aaataacgat
    57841 taaaggatcg acaagagata gcccggctag ttttaaactt tttgccatta attatagcat
    57901 gatgcttttt attaataatt tccgctaatt cgaccggatc gagagcattg gtcatttcat
    57961 caatagagac agttatcggt cctataatgt aacgttttcg ctgatcatca aaagttatcc
    58021 catgatgatt tgccattatt ttgtatttgg ggtcatagaa acatacagga taatctttta
    58081 tcccgtcgta ggtatcctca tcaagtggtt tcatgcgcgt gaagttttgc atatagttac
    58141 cattgcaatt cgtttcatga tttccagcta atacatgaac attttcattg atactcatta
    58201 tgcgttttaa caaatcgatg ataaattgat cgccacagat attactaaat cgatcgccgg
    58261 tttggtcgcc aagaaacgta catggcgtga ctgcgtcttt attaataacc aaataaggct
    58321 gaagactttc taaaacatta tctcttctgt tcagtatact gattattgta ttaacattag
    58381 tgtcaagttg ctcattaaag ttctccctta gcaatacgtt gagaacttca tcttctatat
    58441 taagcaactg gctaaggaaa ttttctcctt cagccatgat tcccaaatgt cctgttgcaa
    58501 aaagcgcgta caaaactgca ccgactgagc catcggtatc gccaaaatag gtcggcttgg
    58561 tacttgtggg atcaacaggt gaacaattgg agaaataggt tctgtcagcg aactggggtt
    58621 ttataattgt ttcgaattta atatcaaata cattgtattg cgatatttcc cagttccaga
    58681 tgggtttgta cgactcagta actgcaacat cattagcatt atcatttact gtcatattat
    58741 taagtaaata aacaatgctg caccaattaa tatcggtatt atcaagaggg caaccgtaat
    58801 ctttactatt aattgtcaca ataaactgat aacagttatt cctacgtaga atatgagcgc
    58861 gcatgcccga tagcatacta tatttttctg aatgattcgt ttgtatggta aatgaaattt
    58921 tgtcatcttg aatagattgt tggaaacgtt ctatttgcca atcatcaatt ttttccacaa
    58981 cttcaccgag cattgtcctg gcaatacttg atatatcatc acctatacca tttgcaatat
    59041 acccaaacag ttctttagtg aagttggtaa catgctcttg caccatatca tttgttgcga
    59101 ttgtcgttgt actgccatta gtgcctgcca cagttccaat tttcatcttt tacctcatat
    59161 tattcaccgc gaggaataca tagttaacat tttatcttat gacatacacc tcatattcga
    59221 tggggaaata attatatttt gcacaaatca ttcagcctta aagcataata tgaaatttct
    59281 tatctcatag cagagaaagt ccctaaagat tgtagaggcg tcatcagtac atacctctac
    59341 aatcgattaa gaaaacctat gaaatactgg caaaattaaa aagagatacc acagcactca
    59401 tgaagtttta ttgaggttga aaacaactaa gccctgggac acctttttta tcaccacgtt
    59461 cgttaggtgc tatcttccaa gaatttagcc ccatggtata attatcaatt ttttgatgct
    59521 ggtctacccc taatccataa tggccaaatt tctgtttagt ttttttgagc gtctgtccac
    59581 atttgaagat atcttcaggt tttggtctga aagcagtagt gttattaaaa taattttcat
    59641 atacaggtgt gggtgctata gcattgcttc taattagatg gagaagatga tcatccatgt
    59701 gttgtttatg ttttttatta catatatttg ctaattcctc aggatcaatt gggtctttca
    59761 tctgattaaa tggaacctgc aaagcaccga gacaataaca ttgattttct tcatttctta
    59821 ttattccgtg atgactggtt aaaactttag tttctgagtt ataattacaa acatcgagcg
    59881 ttttaattaa gttatacgta tcaccggcag agagtttata attttctagt cgtgcagtat
    59941 agttaccatt aaaattgatt tcatgatttc cagccagaac gatgacattt ttattaatac
    60001 ggctatcttt attaccctcc atatttcgca tagaatttag caaagtaagg atatatttat
    60061 caccaaaaat agtactaaat ctatcgcctg tatgatcccc aaggaagata caaggggtta
    60121 tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatcatg tctctcgtat
    60181 ttaataaatt gtagagcaat ttattatttt tatgtatgta tgcagatgaa gccatgtctt
    60241 cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct atatgccctg
    60301 aaacgagcaa tgaataaagt acagcgccta ctcttccatc tgtatcgcca aaataagccg
    60361 tgttggcatt ttgtgggtta atcacaccac attgtttata aaagtctatt ttagaaaaca
    60421 cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag acatcccaac
    60481 gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttcaggt atgatagaaa
    60541 gcccattaac tttataaatt atgtctgatt tatctccgga gaaattaata aaataataac
    60601 catccttaca ctcaatctta actgtaatgg gagacgataa ttttatcaca tggttattgt
    60661 ctggtataat tgtaaagata aacgtgttag tagtattatc ggcaaaacta ttatattctt
    60721 gaatatctat agatggctca ctattgtaaa accatatttt ctccatctga agactatgtt
    60781 catttaccct ccagtgacca tgttgcagga aatagatgat atgcatgcca aaatcgttat
    60841 ctatttgcga atcattatcc agcttatcaa tttcaactat ctttgaacga tgtgttttat
    60901 caaccttgct atggtgttga tgattattaa ctttatctga atgcttatct ttatggtttg
    60961 aagggatgta cttaaatata ttgtcaattg ctttcataaa taacccgcga tatttttgac
    61021 ctgttagtaa aaaataaatg atacatgtct gaaagacata tttacgacga tataaaacaa
    61081 taatacttat atgttcgctg attttaagca ccgagactat ccggtctttt actaaataat
    61141 ggaaaacaat ccatccctgg aagacaattg taaaaaaaaa caacagaata agaattaatc
    61201 ttaggataaa tatttattta tcatggcctt ttgaccgcta aaaaatggca ctcggcacag
    61261 taaaccccgc tatagttatg ctatgcattt gtctttaatg atatctcgtc gttatattaa
    61321 caggctgaaa ttatcattaa tgcacatttc aatattagca gggatacctg aggagtttaa
    61381 agtgaacata tatatcgggt agcttttcaa attaatccct ttgattatag gcttaatttg
    61441 catcgcgtta ggtggctttg tgctggaaag ttcagggcaa cgtcacgggt catgtgctga
    61501 tttctctggc ggccatatgc ctggcattat tcactaccgc atttattatc atttcgcaac
    61561 tcacgcgcgg cgttaatacg ttttacaata cattgttccc cattattggc tatgcggggt
    61621 caattatcac catgatatgg ggttgggcac tgttagcagg taatgatgtg atggcagacg
    61681 agtttgttgc cggccatgtt atttttggcg ttggtatgat tgccgcatgt gtatcgacgg
    61741 tggcagcgtc gtccggtcac tttctgctca ttcccaaaaa tgcagcgggg agcaagagcg
    61801 acggaacacc ggtacaggct tattcttcat taatcggtaa ttgcctcatt gccgttcccg
    61861 ttttactcac cctgctcggt ttcatttggt ctattacgct gttacatagt gccgacataa
    61921 ctccgcatta tgtcgccttg ggttaaccgc aatctgtgcc tgtctaattg gcctagtggc
    61981 cacaatcgtc catcaaacac gtaatacgtt ctcatccaaa gagcactggc tgtggtgtta
    62041 ttgggttatt tttctcggct cagtcacggt gctgcagggg atatacgtct tagtcagttc
    62101 cgatgcaagc gcccgactgg ctcccggcat tattctcatt tgcctcggaa tgatctgtta
    62161 cagcatattc tcaaaagtct ggctactggc actggtctgg agacgtacct gttcgttagc
    62221 caacagaata ccgatgatac cggtatttac ctgtctgttt tgcttgtttt tgtcatcatt
    62281 cctcgcggaa atcgcacaaa ctgagctgga ctactttatt ccatctcggg ttctggttgg
    62341 attaggcgca gtatgtttta cattgttctc tattgtttca atattagagg ccggttctgc
    62401 gaaaaaaaat taaaatttac ataatcccag cgttattcat atcttaacgc tggtttttta
    62461 cgaacttagt gaaataatgt agaatataaa attataattt tatattggag cacatgtgtt
    62521 aatgatcaac cccgggatac taaaaatata gacattatta aacttgctta gcctcgcatt
    62581 gcctttacct gttaaagatg catcgagaaa attgagtctg aagttctcat cctttatctg
    62641 attttccagt cccgaaactt tggccttagc attacctgag atccgtccgt tactatctat
    62701 tttgtaggta aaaaaatacg aatgggaaaa tttcctcggt gtatcttcat cagttaagcc
    62761 aacaatcgta aaactaccgc taccatcttt ttttaaaatg actgaaaggt tagcagagta
    62821 gtataaagaa tgatctgttt tagcatttct tgttaacgtc gacgtacatt caagagacgt
    62881 tgtgctacca tcacgagcct tccagagagc aaaaaaacac acctgtagca atgtgaaaac
    62941 aagtattaat ataaaataca ttttcttatt agtcatataa tagcacctct gaacaaattg
    63001 cataagtttc gtcattagag gattttatac atcttgataa ataccgctgc ccttccccaa
    63061 gcaaataatg catactattt acatcatata taaaatttgc acctggcaca cagggtaaat
    63121 gctttttcga aatttcagcg gcctctattt gagatttagc gaggccaatc tcgttaagat
    63181 tctctgaaag atataaatca caggtgtcaa tcttacctga tttaaccaat ggataatccg
    63241 ttatactgta ggtatcttta aaagccacgg caaatatttc aataataaca attactacca
    63301 caagcgaaat caaaatgcga aaaagaggaa tactcctttt gcgctctact ttttcaacaa
    63361 catcttcttg taaatgagaa tcatcataag attgtttact tattgttatg ttttcactga
    63421 tgtaaaagcc tactttgggt atcgtaagaa tcacattttc ttcaacacca atatttttaa
    63481 aacttttgcg taacagggat atatattggt tcaatgtgtt gtttgacaga tcaaatgaaa
    63541 atctctccaa aacgctatca atgattgagt tcctgtcaac cgctccacca ttactttcca
    63601 gtaacactgc caaaatacac cccgccgtag ctggcaaatt tatctcatcc tgggtattta
    63661 cattcgagag cgagcatttg ttcggatcaa aaataatcgc gtcattgata atgaaaaaac
    63721 atctccgctc catgcaggca acaacacaaa ataagatttt gtcattttag cctattcctg
    63781 gtggaactga cacaagaaac taaaatacaa atataaccct gaaaaagtca tataaaactt
    63841 atatccacag aataaaaaat caaacatgtc agttttatca atataaagta tcgcaaggcg
    63901 aaaagtgatg caagaatact cctaagtaaa tgaaagcgca ccacatgaaa ttatcaacac
    63961 atgtaatttc atcattaggt gctatcgaaa ataactcagg agctacttaa gatgaataat
    64021 gtcttactca tgagacccga tgactcagaa tttatgatac ctgatgactg gtcagggcta
    64081 aacgcaaaag gcattacata cgaacaagat aatagagtga cagagtcttt tctgcctttt
    64141 aaatcttttt attgttcacc ggtgtgcacg acagttgaac tcatctcaca aaataattgt
    64201 tttgctatgg gtatccgcta ccttctggaa aagaaaagag acttaaggct aaatcacttt
    64261 aaagccttct ctcataaatg cgagacaaaa cctgacgtgt taattatgac cgggtgcacc
    64321 gtcgctgata cttatagtat attctcacgc attgttgaag aaaatataat gaaaacgatg
    64381 acacctatcg tcatcattac tgggcatcac tcagaagatc tttgttctct ggcatcgaaa
    64441 ctgggaggag ttaaaataat atcaccgaca gaaaagattg caaaaataga aaacgctatc
    64501 aatgattctt ttaacagcaa gcctaaaaaa ataagatttt taacagatat actctctcct
    64561 caacaaattg agaccatgaa aatggttgcg cagggtagat ccgttaatga gatttcagcc
    64621 gcaatgaata ttagcatcaa gaccgtgtat gcacataaag ttcgtgctat caataagctg
    64681 ggaattaaga aaaaagttta tgaagcgctt ttttataaag cgctaattca gttcaactga
    64741 tgtaaaaatc accctcgctt aataaataat atattctatg tcttttttta taagttaaaa
    64801 aaggcgggat atcaaacccc gccatgaaat taactaatca tctgcattat tctgcgtaga
    64861 tataagcctg ggtacggatg gtgtaagagt ctggctcaac aacctgttct ttcatatggg
    64921 aaacgacgcg ataggcacca cctgttccag tatttttcag catcatataa cgcttaccgc
    64981 caatctcttc ggaggcagct gaaggaggat cctgctcaag cgttgtcagg gaaactttca
    65041 gtttatggtc cgggttattg tcaccagaaa ttactgcatc cgctgaagta ctgctctccg
    65101 cataaatatc atccgggaaa gttacagcat agtcgtggct ctgcccatca gatgcgccaa
    65161 caatacctgt tgccaaaggc gtccatgcag ttaacgtacc gctatatacc cctttcactg
    65221 gttgaatggt attattaaca acgataggct tggtcaaagt aagattagct gacgcttcag
    65281 taccctggct tactaaagtt cccgcctgag aagcaaaagc tgtccccgcc agcatgattg
    65341 ctaacaatgt tttcttcatt atattttctc catgattttc atttacactg caaaatgcag
    65401 tttctttctt tgcatttacg tgctatcgcg aaaatgcttc caccaaataa tggaaacatc
    65461 ttcacaaaaa taaattatga gtttcttaat ttagaaacta taaacatgtc gccgctatct
    65521 ggtataaccc tgatgcactt ttcttcgcag gaagttggta ttttgcgata caggattgat
    65581 ctttagaata accccatttc acattaaact cgcct
//
LOCUS       DAJVRP010000009        60449 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVRP010000009 DAJVRP010000000
VERSION     DAJVRP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.009"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..322
                     /locus_tag="OQN12_000556"
     CDS             <1..322
                     /locus_tag="OQN12_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1653019.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="OQN12_000557"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="OQN12_000557"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1653020.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="OQN12_000558"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="OQN12_000558"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1653021.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OQN12_000559"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OQN12_000559"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1653022.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="OQN12_000560"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="OQN12_000560"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1653023.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="OQN12_000561"
     CDS             complement(3726..4955)
                     /locus_tag="OQN12_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1653024.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="OQN12_000562"
     CDS             complement(5019..5459)
                     /locus_tag="OQN12_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1653025.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="OQN12_000563"
     CDS             complement(5564..6334)
                     /locus_tag="OQN12_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1653026.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="OQN12_000564"
     CDS             complement(6331..7257)
                     /locus_tag="OQN12_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1653027.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVEHKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="OQN12_000565"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="OQN12_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1653028.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OQN12_000566"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OQN12_000566"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1653029.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="OQN12_000567"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="OQN12_000567"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1653030.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OQN12_000568"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OQN12_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS1653031.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="OQN12_000569"
     CDS             12964..13311
                     /locus_tag="OQN12_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1653032.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="OQN12_000570"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="OQN12_000570"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1653033.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="OQN12_000571"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="OQN12_000571"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1653034.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="OQN12_000572"
     CDS             complement(15131..15493)
                     /locus_tag="OQN12_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1653035.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OQN12_000573"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OQN12_000573"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1653036.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="OQN12_000574"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="OQN12_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OQN12_000575"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OQN12_000575"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1653037.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OQN12_000576"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OQN12_000576"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1653038.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OQN12_000577"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OQN12_000577"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1653039.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OQN12_000578"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OQN12_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1653040.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="OQN12_000579"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="OQN12_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1653041.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OQN12_000580"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OQN12_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1653042.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OQN12_000581"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OQN12_000581"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1653043.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="OQN12_000582"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="OQN12_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1653044.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="OQN12_000583"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="OQN12_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS1653045.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="OQN12_000584"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="OQN12_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="OQN12_000585"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="OQN12_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCS1653046.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OQN12_000586"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OQN12_000586"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1653047.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="OQN12_000587"
     CDS             complement(36348..36386)
                     /locus_tag="OQN12_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653048.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="OQN12_000588"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="OQN12_000588"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1653049.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="OQN12_000589"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="OQN12_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1653050.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="OQN12_000590"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="OQN12_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1653051.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OQN12_000591"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OQN12_000591"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1653052.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OQN12_000592"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OQN12_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1653053.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OQN12_000593"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OQN12_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1653054.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OQN12_000594"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OQN12_000594"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1653055.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OQN12_000595"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OQN12_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1653056.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OQN12_000596"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OQN12_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1653057.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OQN12_000597"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OQN12_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1653058.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OQN12_000598"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OQN12_000598"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1653059.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OQN12_000599"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OQN12_000599"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1653060.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OQN12_000600"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OQN12_000600"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1653061.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OQN12_000601"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OQN12_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1653062.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OQN12_000602"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OQN12_000602"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1653063.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OQN12_000603"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OQN12_000603"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1653064.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OQN12_000604"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OQN12_000604"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1653065.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OQN12_000605"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OQN12_000605"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1653066.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OQN12_000606"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OQN12_000606"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1653067.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OQN12_000607"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OQN12_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1653068.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OQN12_000608"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OQN12_000608"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1653069.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OQN12_000609"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OQN12_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS1653070.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14483 a        16739 c        14854 g        14373 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg cccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccaag gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgtg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgaacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagcg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ttccttgatg taaataaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactggac gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagaattg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggcaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcgcatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcaa
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac agctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgactctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgatg
    60421 tccggtaatg actccaactt actgatagt
//
LOCUS       DAJVRP010000010        59843 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVRP010000010 DAJVRP010000000
VERSION     DAJVRP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.010"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            531..1820
                     /gene="sdaC"
                     /locus_tag="OQN12_000610"
     CDS             531..1820
                     /gene="sdaC"
                     /locus_tag="OQN12_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1653071.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1878..3245
                     /gene="sdaB"
                     /locus_tag="OQN12_000611"
     CDS             1878..3245
                     /gene="sdaB"
                     /locus_tag="OQN12_000611"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1653072.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3356..4111
                     /gene="xni"
                     /locus_tag="OQN12_000612"
     CDS             3356..4111
                     /gene="xni"
                     /locus_tag="OQN12_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1653073.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4164..5312)
                     /gene="fucO"
                     /locus_tag="OQN12_000613"
     CDS             complement(4164..5312)
                     /gene="fucO"
                     /locus_tag="OQN12_000613"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCS1653074.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5340..5987)
                     /gene="fucA"
                     /locus_tag="OQN12_000614"
     CDS             complement(5340..5987)
                     /gene="fucA"
                     /locus_tag="OQN12_000614"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1653075.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6534..7850
                     /gene="fucP"
                     /locus_tag="OQN12_000615"
     CDS             6534..7850
                     /gene="fucP"
                     /locus_tag="OQN12_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1653076.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7883..9658
                     /gene="fucI"
                     /locus_tag="OQN12_000616"
     CDS             7883..9658
                     /gene="fucI"
                     /locus_tag="OQN12_000616"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCS1653077.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9767..11186
                     /gene="fucK"
                     /locus_tag="OQN12_000617"
                     /pseudo
     CDS             9767..11186
                     /gene="fucK"
                     /locus_tag="OQN12_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11188..11610
                     /gene="fucU"
                     /locus_tag="OQN12_000618"
     CDS             11188..11610
                     /gene="fucU"
                     /locus_tag="OQN12_000618"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS1653078.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11668..12399
                     /gene="fucR"
                     /locus_tag="OQN12_000619"
     CDS             11668..12399
                     /gene="fucR"
                     /locus_tag="OQN12_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1653079.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12443..13543)
                     /gene="rlmM"
                     /locus_tag="OQN12_000620"
     CDS             complement(12443..13543)
                     /gene="rlmM"
                     /locus_tag="OQN12_000620"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1653080.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13536..13931)
                     /locus_tag="OQN12_000621"
     CDS             complement(13536..13931)
                     /locus_tag="OQN12_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1653081.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13950..14867)
                     /gene="gcvA"
                     /locus_tag="OQN12_000622"
     CDS             complement(13950..14867)
                     /gene="gcvA"
                     /locus_tag="OQN12_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1653082.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15218..15445)
                     /locus_tag="OQN12_000623"
     CDS             complement(15218..15445)
                     /locus_tag="OQN12_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1653083.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15637..16842
                     /gene="csdA"
                     /locus_tag="OQN12_000624"
     CDS             15637..16842
                     /gene="csdA"
                     /locus_tag="OQN12_000624"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1653084.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16842..17285
                     /gene="csdE"
                     /locus_tag="OQN12_000625"
     CDS             16842..17285
                     /gene="csdE"
                     /locus_tag="OQN12_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1653085.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17336..18142)
                     /gene="tcdA"
                     /locus_tag="OQN12_000626"
     CDS             complement(17336..18142)
                     /gene="tcdA"
                     /locus_tag="OQN12_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1653086.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18381..19478)
                     /gene="mltA"
                     /locus_tag="OQN12_000627"
     CDS             complement(18381..19478)
                     /gene="mltA"
                     /locus_tag="OQN12_000627"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1653087.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19687..19763
                     /locus_tag="OQN12_000628"
     tRNA            19687..19763
                     /locus_tag="OQN12_000628"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19721..19723,aa:Met,seq:cat)
     gene            19797..19873
                     /locus_tag="OQN12_000629"
     tRNA            19797..19873
                     /locus_tag="OQN12_000629"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19831..19833,aa:Met,seq:cat)
     gene            complement(19948..21201)
                     /gene="amiC"
                     /locus_tag="OQN12_000630"
     CDS             complement(19948..21201)
                     /gene="amiC"
                     /locus_tag="OQN12_000630"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1653088.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21433..22764
                     /gene="argA"
                     /locus_tag="OQN12_000631"
     CDS             21433..22764
                     /gene="argA"
                     /locus_tag="OQN12_000631"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1653089.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22826..24652)
                     /gene="recD"
                     /locus_tag="OQN12_000632"
     CDS             complement(22826..24652)
                     /gene="recD"
                     /locus_tag="OQN12_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1653090.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24652..28194)
                     /gene="recB"
                     /locus_tag="OQN12_000633"
     CDS             complement(24652..28194)
                     /gene="recB"
                     /locus_tag="OQN12_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1653091.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28187..31075)
                     /gene="ptrA"
                     /locus_tag="OQN12_000634"
     CDS             complement(28187..31075)
                     /gene="ptrA"
                     /locus_tag="OQN12_000634"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1653092.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31251..34619)
                     /gene="recC"
                     /locus_tag="OQN12_000635"
     CDS             complement(31251..34619)
                     /gene="recC"
                     /locus_tag="OQN12_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1653093.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34632..34964)
                     /locus_tag="OQN12_000636"
     CDS             complement(34632..34964)
                     /locus_tag="OQN12_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1653094.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34949..35356)
                     /locus_tag="OQN12_000637"
     CDS             complement(34949..35356)
                     /locus_tag="OQN12_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1653095.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35353..35916)
                     /locus_tag="OQN12_000638"
     CDS             complement(35353..35916)
                     /locus_tag="OQN12_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1653096.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35907..36377)
                     /locus_tag="OQN12_000639"
                     /pseudo
     CDS             complement(35907..36377)
                     /locus_tag="OQN12_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36561..37355)
                     /gene="thyA"
                     /locus_tag="OQN12_000640"
     CDS             complement(36561..37355)
                     /gene="thyA"
                     /locus_tag="OQN12_000640"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1653097.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37362..38237)
                     /gene="lgt"
                     /locus_tag="OQN12_000641"
     CDS             complement(37362..38237)
                     /gene="lgt"
                     /locus_tag="OQN12_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1653098.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38388..40634)
                     /gene="ptsP"
                     /locus_tag="OQN12_000642"
     CDS             complement(38388..40634)
                     /gene="ptsP"
                     /locus_tag="OQN12_000642"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1653099.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40647..41177)
                     /gene="rppH"
                     /locus_tag="OQN12_000643"
     CDS             complement(40647..41177)
                     /gene="rppH"
                     /locus_tag="OQN12_000643"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1653100.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41862..42551
                     /gene="mutH"
                     /locus_tag="OQN12_000644"
     CDS             41862..42551
                     /gene="mutH"
                     /locus_tag="OQN12_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1653101.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLRKDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42620..43333
                     /locus_tag="OQN12_000645"
     CDS             42620..43333
                     /locus_tag="OQN12_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1653102.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43471..43689
                     /gene="ygdR"
                     /locus_tag="OQN12_000646"
     CDS             43471..43689
                     /gene="ygdR"
                     /locus_tag="OQN12_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS1653103.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43797..44837
                     /locus_tag="OQN12_000647"
     CDS             43797..44837
                     /locus_tag="OQN12_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1653104.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44870..46060)
                     /gene="lplT"
                     /locus_tag="OQN12_000648"
     CDS             complement(44870..46060)
                     /gene="lplT"
                     /locus_tag="OQN12_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1653105.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46053..48212)
                     /gene="aas"
                     /locus_tag="OQN12_000649"
     CDS             complement(46053..48212)
                     /gene="aas"
                     /locus_tag="OQN12_000649"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1653106.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48798..49829
                     /gene="galR"
                     /locus_tag="OQN12_000650"
     CDS             48798..49829
                     /gene="galR"
                     /locus_tag="OQN12_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1653107.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49836..51098)
                     /gene="lysA"
                     /locus_tag="OQN12_000651"
     CDS             complement(49836..51098)
                     /gene="lysA"
                     /locus_tag="OQN12_000651"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1653108.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51220..52155
                     /gene="lysR"
                     /locus_tag="OQN12_000652"
     CDS             51220..52155
                     /gene="lysR"
                     /locus_tag="OQN12_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1653109.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52142..52834)
                     /gene="ygeA"
                     /locus_tag="OQN12_000653"
     CDS             complement(52142..52834)
                     /gene="ygeA"
                     /locus_tag="OQN12_000653"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1653110.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52963..54381)
                     /gene="araE"
                     /locus_tag="OQN12_000654"
     CDS             complement(52963..54381)
                     /gene="araE"
                     /locus_tag="OQN12_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCS1653111.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54696..55457)
                     /gene="kduD"
                     /locus_tag="OQN12_000655"
     CDS             complement(54696..55457)
                     /gene="kduD"
                     /locus_tag="OQN12_000655"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1653112.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55487..56323)
                     /gene="kduI"
                     /locus_tag="OQN12_000656"
     CDS             complement(55487..56323)
                     /gene="kduI"
                     /locus_tag="OQN12_000656"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1653113.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56610..57791)
                     /locus_tag="OQN12_000657"
     CDS             complement(56610..57791)
                     /locus_tag="OQN12_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1653114.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58046..59275
                     /locus_tag="OQN12_000658"
                     /pseudo
     CDS             58046..59275
                     /locus_tag="OQN12_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14776 a        16051 c        14574 g        14442 t
ORIGIN      
        1 gccgggtttt ctcgtttttg gtcacttact catcaactca tttcatttgt tatatgaatg
       61 tttcttacca ccctcacgcg acaaatatca tcacagttaa tatgtcatac aatttatgtt
      121 gcaacacaaa cgtttcccta ttttcataaa ccgtatttta tcgctatgaa aaagaaatta
      181 tcgccatgat taactaaaag tattgatttt ttcagttcaa cctacatata ttgcgcgccc
      241 cggaagaagt cagatgtcgt ttaatgggca aatattgccc ttaaattctc ttttactttt
      301 gatttacaga gtaaagcgtt gggataatct atcttccaag cagattattg tatttgggat
      361 caagatcact gatagataca taacttgtgt gtatctttcc gccctcaaat tattacggcg
      421 gtaaatgatt aagccatcgc cgatagacag atttcatttt tacggtcagg caccttccct
      481 ggctgaactg gctaaaagct gaattatttg cattcctcca ggagaaatag atggaaacga
      541 ctcaaaccag cacgattgcg tcgaaagact ctcgtagtgc ctggcgcaag acagacacca
      601 tgtggatgct gggcctttac ggcacggcaa tcggcgcggg cgtgctgttc ctgccaatca
      661 acgccggtgt tggcggtatg atcccgctga tcatcatggc tatccttgcg ttcccgatga
      721 cgttttttgc tcaccgcggc ctgactcgct tcgtactgtc tggtaaaaac ccgggcgaag
      781 acatcaccga ggttgtagaa gaacactttg gtattggcgc aggtaaactg attaccctgc
      841 tctacttctt cgctatctac ccgatcctgc tggtttatag cgtggcaatc accaataccg
      901 ttgaaagctt catgtctcac cagctgggta tgacgccacc gccgcgtgcg attctgtcgc
      961 tgatcctgat cgtgggtatg atgaccatcg ttcgctttgg tgagcagatg atcgttaaag
     1021 cgatgagtat tctggtattc ccgtttgttg gcgtactgat gctgctggct ctgtacctga
     1081 tcccgcagtg gaacggcgca gcactggaaa cgctgtctct ggacactgca tctgcaaccg
     1141 gaaacggtct gtggatgacc ctgtggctgg caattccggt aatggtgttc tcgttcaacc
     1201 actctccgat catctcttct ttcgccgttg cgaagcgtga agagtacggc gatatggcag
     1261 aacagaaatg ctcgaagatc ctggcattcg cacacatcat gatggtgctg accgtaatgt
     1321 tcttcgtctt cagctgcgta ctgagcctga ctccggcaga cctggctgcg gctaaagagc
     1381 agaacatctc gattctgtct tacctggcta accactttaa cgcaccggtt atcgcgtgga
     1441 tggctccgat tatcgcgatt atcgctatca ccaaatcctt cctcggccac tacctgggcg
     1501 cacgtgaagg cttcaacggt atggtgatta aatctctgcg tggtaaaggt aagtctatcg
     1561 aaatcaacaa gctgaaccgt atcactgcgc tgttcatgct ggtaacgacc tggattgttg
     1621 ccaccctgaa cccgagcatc ctgggtatga ttgaaaccct gggcggccca atcatcgcga
     1681 tgatcctgtt cctgatgccg atgtacgcaa ttcagaaagt accggcaatg cgtaagtaca
     1741 gcggtcacat cagcaacgta ttcgttgtcg tgatgggtct gattgcaatc tccgcaatct
     1801 tctactctct gttcagctaa gtcctttcgc gccgctttcg ggcggcgctt cctccgtttt
     1861 aacgcgatgt atttcctatg attagcgtat tcgatatttt caaaatcggc attggccctt
     1921 ccagttctca taccgttgga ccaatgaaag cgggtaaaca atttaccgac gatctgattg
     1981 cccgtaacct gcttaaagac gtgacccgcg tggtggttga cgtgtacggc tcgctctctc
     2041 tgactggtaa aggccaccac acagatatcg ccattattat gggcctggcg ggtaacctgc
     2101 cggataccgt ggatatcgat tccatccccg gttttattca ggatgtgaat actcatggtc
     2161 gcctgatgct ggcaaacggt cagcatgaag tggagttccc ggttgatcag tgcatgaact
     2221 tccatgcaga taacctttct ctgcatgaaa acggtatgcg cattaccgcg ctggcgggcg
     2281 ataaagtcgt ttacagccag acttactact ctattggcgg tggctttatc gttgatgaag
     2341 agcattttgg ccagcaggat agcgcaccgg ttgaagttcc ttatccgtac agttcagcag
     2401 ccgatctgca aaaacattgt caggaaaccg ggctgtcact ctctggcctg atgatgaaaa
     2461 acgagctggc gctgcacagt aaagaagagc tggaacagca cctggcgaac gtctgggaag
     2521 tcatgcgtgg cggtattgag cgcggtattt ccaccgaagg cgtgttgcct ggcaaactgc
     2581 gcgttccacg ccgtgctgcg gcactacgcc ggatgctggt cagcctggat aaaaccacca
     2641 ctgacccgat ggcggttgtt gactggatca acatgtttgc actggcagtg aacgaagaga
     2701 acgctgctgg cggtcgcgtg gtgactgcgc cgactaacgg tgcgtgcggg attatcccgg
     2761 ctgtgctggc gtactacgat aagtttatcc gcgaagtgaa cgctaactca ctggctcgtt
     2821 acctgctggt agccagcgcc attggttctc tttataagat gaacgcgtcg atttctggtg
     2881 ccgaagtggg ttgccagggt gaagttggcg tggcgtgctc tatggcggca gctggtctgg
     2941 cagaactgtt aggcgcaagc ccggcgcagg tttgcatcgc agcggaaatc gcgatggagc
     3001 acaacctcgg tctgacttgt gacccggtcg cggggcaggt tcaggtaccg tgcattgagc
     3061 gtaacgccat tgcagcagtg aaagcggtga acgccgcgcg tatggcactg cgccgtacca
     3121 gcgaaccgcg cgtctgcctc gataaagtta tcgaaaccat gtacgaaaca ggtaaagaca
     3181 tgaacgccaa gtaccgcgaa acctctcgcg gcggcctggc gatgaagatc gttgcctgcg
     3241 attaatctct cccagaggcc tcgttttgcg aagcctcttc ctgttttctc aaccagccat
     3301 agcctgttcc ggcatcgaat gttaccctta tcgcctgatc tttaaggggg ttatcgtggc
     3361 tgttcatttg cttattgtcg atgcactgaa tcttatccgt cgcattcatg ccgttcaggg
     3421 ttcgccctgt gtcgaaacct gccagcatgc gctcgatcag ctcattatgc acagccagcc
     3481 aacccacgcg gtcgccgttt ttgatgatga aaaccgcagt agcggctggc gtcatcagcg
     3541 tttaccagat tacaaagcgg atcgaccgcc aatgccggaa gagttgcacg acgagatgcc
     3601 tgcattacgc gccgcctttg agcaacgcgg cgtcccgtgc tggtcagcca gcggcaacga
     3661 agccgatgac ttagccgcca cactggcggt caaagtgaca caggccgggc atcaggcaac
     3721 gattgtttcg acagataaag gctactgtca gttactttca ccgacattac gtattcgtga
     3781 ttacttccag aaacgttggc tggatgcgcc atttatcgat aaagaatttg gcgttcaacc
     3841 gcagcagttg cccgattact ggggacttgc ggggatcagc agttcaaagg taccgggtgt
     3901 tgcgggaatc ggaccaaaaa gcgccacgca gctgctggtc gagtttcaga gtctggaagg
     3961 gatatatgag aatctggatg cggttgccga aaagtggcgc aaaaaattag aaacccataa
     4021 agagatggcg tttctgtgcc gcgatattgc ccgcttacaa accgatttgc atatcgacgg
     4081 caatttacag caattgcggt tggtacggta acggcgagcc ggatacgccg caaatcgtat
     4141 ccggcattat cacatcagcg catttaccag gcggtatggt aaagctctac aatatcctca
     4201 agcgttgctt cacgcgggtt gccaccggta caaacatcat ccagtgccgc ctgagccagt
     4261 gccgaaatgt cttccttgcg tacaccaaca tcacgcaaat gtggcggaat accgacatca
     4321 cggttgaggg caaacaccgc ttcaacagcg gcattacgcg ccccttccag gctcatacct
     4381 tccactttca cgcccataac gcgcgcgata tcgcggtact tctcaccggt aaagtcagcg
     4441 ttatagcgca tgacatgcgg taacaggatg gcgttcgcaa caccgtgtgg agtgttataa
     4501 aacgcgccca gtggatgcgc cataccatgc accaacccta acccaacatt cgagaagccc
     4561 atacccgcaa catactgccc gagcgccatt tcttctccgg catccttatc accagcaacc
     4621 gatcctcgca gcgccccagc aatgatttca atcgctttaa tgtgcagtgc atcggttagt
     4681 gcccacgcgc cacgggtaat atacccctca atagcatgag tgagcgcatc aacacccgtc
     4741 gcggctttca gcgccggagg cataccatcc atcatgtcag catcaataaa cgccacctgc
     4801 gggatatcat gcggatcaac gcaaacaaac ttgcgccgtt tttcttcgtc agtgatcacg
     4861 tagttaatgg tcacttctgc cgcagtacct gctgtggtgg ggattgccag aatcggtaca
     4921 ctgggtttat tggtcgggga aagcccttcc aggctacgca catcggcaaa ctccgggttg
     4981 ttgctgataa tgccaattgc tttacaagta tcctgtggag aaccaccacc aatagcgatc
     5041 aggtaatccg cgccgctatt ctggaataca ccgagccctt ctttgacgac agtaattgtt
     5101 gggttgggca ctacgccgtc gtaaatcgcc catgccagcc ctgcagcatc catcttatcg
     5161 gtcactttcg ccaccacgcc gcattgcacc agcgttttat cggtgacgat cagcgccttc
     5221 tgataaccac ggcgtttcac ctcatcggtt aaagccccaa cagcaccccg accaaaccat
     5281 gccgtttcgt tcagaatcat tctgttagcc atcatccttc tccttgttgc tttacgaaat
     5341 tactcttcaa tccgtaaccc ataggttttg aatttctcca gcactacggc aatctcttca
     5401 tcgctcagca ctggcaccgg gtccgtaatc gccagggtcg tcaggtaaag ttgcgccagc
     5461 acttcaactt catgcgccag ccataacgct ttttccagat tcacctcaca agcgataagc
     5521 ccatgatgtt gtaacaaagt tgccttacga tttttgagag ccagcgcaac atgttcagaa
     5581 agttcgcgtg ttccaaaggt cgcataaggc gcgcaaggaa tagaattacc gccagccgcc
     5641 gcaatcatgt agtgaatagc ggggatcgat cggttaagaa tggaaactgc cgtgcaatga
     5701 acggcatgat tgtgaacaac cgcgttggca tccggtctgc tttgataggc tgccatatgg
     5761 aaacgccatt cgcttgaggg gagctttcct tcctcatgtt taccgttgcc atcaataaag
     5821 acaatatgcg actccgtcag tttttcatat ggaatgcctg taggcgtaat cagcatccca
     5881 tcctgataac gtacactgac gttccccgct gtcccctggt tcagtcccag gcgggtcatt
     5941 tccaggcaag tgtcaataat ctgacgagca agtttatttc gttccattag ctacctctct
     6001 ctgattcaaa acagggcaat aatgttgttc ctttcacact attgaattag ccgtttaatt
     6061 acccaccatc ttcttcctga ttaacaagaa agaaattcac aagcttatat tttgtgacct
     6121 agctcaacta atcacagtaa ataactgcaa gttctctttt tataaccccg ttaaaaatga
     6181 ccgctcttaa aaatatttat caaaacggtc atttttccat ttcccctcgc cagaaatgcc
     6241 cgttttcggc acaaacaatt aatacggtca tctggtttgt gttttttatg atttattttc
     6301 tgaaacgggc atgaaatttc gattattaaa gtgatggtag tcacataaag tcaccttcca
     6361 gctaataagt gtgaccgccg tcatattaca gagcgttttt tatttgaaaa tgaatccatg
     6421 agttcatttc agacaggcaa atattcaccg atatgaagcc cgaactcgct ggttttgcac
     6481 ttttgaaaac ataaccgatt acgtgcttaa gcttctgaac ctaagaggat gctatgggaa
     6541 acacatcaat acaaacgcag agttaccgtg cggtagataa agatgcaggg caaagcagaa
     6601 gttacattat tccattcgcg ctgctgtgct cactgttttt tctttgggcg gtagccaata
     6661 accttaacga cattttatta cctcaattcc agcaggcttt tacgctgaca aatttccagg
     6721 ctggcctgat ccaatcggcc ttttactttg gttatttcat tatcccaatc cctgctggga
     6781 tattgatgaa aaaactcagt tataaagcag ggattattac cggattattt ttatatgcct
     6841 tgggcgctgc attattctgg cccgctgcag aaataatgaa ctacacctta tttttagttg
     6901 gcctatttat tattgcagcc ggattaggtt gtctggaaac tgccgcaaac ccttttgtta
     6961 cggtattagg gccggaaagc agtagtcact tccgcttaaa tcttgcgcaa acatttaact
     7021 cgtttggcgc aattatcgcg gttgtctttg ggcaaagtct tattttgtct aacgtgccac
     7081 atcaatcgca agacgttctc gataaaatgt ctccagagca attgagtgcg tataaacaca
     7141 gcctggtatt atcggtacag acaccttata tgatcatcgt ggctatcgtg ttactggtcg
     7201 ccctgctgat catgctgacg aaattcccgg cattgcagag tgataatcac agtgacgcca
     7261 aacaaggatc gttctccgca tcgctttctc gtctggcgcg tattcgtcac tggcgctggg
     7321 cggtattagc gcaattctgc tatgtcggcg cacaaacggc ctgctggagc tatttgattc
     7381 gctacgctgt agaagaaatt ccaggtatga ctgcaggctt tgccgctaac tatttaaccg
     7441 gaaccatggt gtgcttcttt attggtcgtt tcaccggtac ctggctcatc agtcgcttcg
     7501 caccacacaa agtcctggcc gcctacgcat taatcgctat ggcactgtgc ctgatctcag
     7561 ccttcgctgg cggtcatgtg ggcttaatag ccctgacttt atgcagcgcc tttatgtcga
     7621 ttcagtaccc aacaatcttc tcgctgggca ttaagaatct cggccaggac accaaatacg
     7681 gttcgtcctt catcgttatg accattattg gcggcggtat tgtcactccg gtcatgggtt
     7741 ttgtcagtga cgcggcgggc aacatcccca ctgctgaact gatccccgca ctctgcttcg
     7801 cggtcatctt tatctttgcc cgtttccgtt ctcaaacggc aactaactga acatattttc
     7861 cgaataaagt gaggaatctg taatgaaaaa aatcagctta ccgaaaattg gtatccgccc
     7921 ggttattgac ggtcgtcgca tgggtgttcg tgagtcgctt gaagaacaaa caatgaatat
     7981 ggcgaaagct acggccgcac tgctgaccga gaaactgcgc catgcctgcg gagctgccgt
     8041 cgagtgtgtc atttccgata cctgtatcgc gggtatggct gaagccgctg cttgcgaaga
     8101 aaaattcagc agccagaatg taggcctcac cattacggta acgccttgct ggtgctatgg
     8161 cagtgaaacc atcgacatgg atccaacccg cccgaaagcc atttggggct ttaacggcac
     8221 tgaacgcccc ggcgctgttt acctggcagc ggctctggca gctcacagcc agaaaggcat
     8281 cccggcattc tccatttacg gtcatgacgt tcaggatgcc gatgacacat cgattcctgc
     8341 cgatgttgaa gaaaaactgc tgcgctttgc ccgcgccggt ttggccgtcg ccagcatgaa
     8401 aggtaaaagc tatctgtcgc tgggcggcgt ttcgatgggt atcgccggtt ctattgttga
     8461 tcacaacttc tttgaatcct ggctgggaat gaaagtccag gcggtggata tgaccgaact
     8521 gcgtcgccgt atcgatcaga agatttacga cgaagccgaa ctggaaatgg cactggcctg
     8581 ggctgataaa aacttccgct atgacgaaga tgaaaataac aaacagtatc aacgcaatgc
     8641 cgagcaaagc cgcgcagttc tgcgcgaaag tttactgatg gcgatgtgta tccgcgacat
     8701 gatgcaaggc aacagcaaac tggccgatat tggtcgcgtg gaagaatcac ttggctacaa
     8761 cgccatcgct gcgggcttcc aggggcaacg tcactggacc gatcaatatc ccaatggtga
     8821 caccgccgaa gcgatcctca acagttcatt tgactggaat ggcgtgcgcg aaccctttgt
     8881 cgtggcgacc gaaaacgaca gtcttaacgg cgtggcaatg ctaatgggtc accagctcac
     8941 cggcaccgct caggtatttg ccgatgtgcg tacctactgg tcaccagaag caattgagcg
     9001 tgtaacgggg cataaactgg acggactggc agaacacggc atcatccatt tgatcaactc
     9061 aggttctgct gcgctggacg gttcctgtaa acaacgcgac agcgaaggta acccgacgat
     9121 gaagccgcac tgggaaatct ctcagcaaga ggctgacgct tgcctcgccg ctaccaaatg
     9181 gtgcccggcg atccacgaat acttccgtgg cggcggttac tcttcccgct tccttaccga
     9241 aggcggcgtc ccgttcacca tgactcgtgt caacatcatc aaaggcctgg gaccggtact
     9301 gcaaatcgcg gaaggctgga gcgtggaatt gccgaaggat gtgcatgaca tcctcaacaa
     9361 acgcaccaac tcaacctggc caaccacctg gtttgcaccg cgcctcactg gtaaagggcc
     9421 gtttacggat gtgtattcgg taatggcgaa ctggggtgcc aaccacggag ttctgaccat
     9481 cggccacgtt ggcgcagact ttatcaccct tgcctccatg ctgcgtatcc cggtatgtat
     9541 gcacaacgtt gaagagacca aagtgtatcg tccttctgcc tgggctgcgc acggcatgga
     9601 tattgaaggc caggattacc gcgcttgcca gaactacggt ccgttgtaca agcgttaata
     9661 cattacaccc tgatgtgatg cccggtcgct ccggctaccg ggcctgaaca agcaagagtg
     9721 gttagccgga taaacaatgt tatccggcta tattgcagga gcgattatga aacaagaagt
     9781 tatcctggta ctcgactgtg gcgcgaccaa tgtcagggcc atcgcggtta atcggcaggg
     9841 aaaaattgtt gcccgcgcct caacgcctaa tgccagcgat atcgcgatgg aaaacaacac
     9901 ctggcaccag tggtctttag actgccattt tgcaacgctt tgctgattgc tgtcggcaaa
     9961 tcaatagtga actgactgaa tgccacatcc gcggtatcgc cgtcaccacc tttagtgtgg
    10021 atggcgctct ggtagataag caaggcaatc tgctctatcc gatcattagc tggaaatgtc
    10081 cgcgaacagc agcggttatg gacaatattg aacggttaat ctccgcacag cggttgcagg
    10141 ctatttctgg cgtcggagcc tttagtttca atacgttata taagttggtg tggttgaaag
    10201 aaaatcatcc acaactgctg gaacgcgcgc acgcctggct cttcatttcg tcgctgatta
    10261 accaccgttt aaccagcgaa ttcactactg atatcacgat ggccggaacc agccagatgc
    10321 tggatatcca gcaacgcgat ttcagtccgc aaattttaca agccaccggt attccacgcc
    10381 gactcttccc tcgtctggtg gaagcgggtg aacagattgg tacgctacag aacaacgccg
    10441 cagcaatgct cggcttaccc gtcggcatac cggtgatttc cgcaggtcac gatacccagt
    10501 tcgccctttt tggcgctggt gccgaacaaa atgaacccgt gctctcctcc ggtacatggg
    10561 aaattttaat ggttcgcagc gcctaggttg atacttcgct gttaagtcag tacgccggtt
    10621 ccacctgcga actagatagc caggcatggt tgtataaccc aggtatgcaa tggctggcat
    10681 ccggcgtgct ggaatgggtg agaaaactgt tctggacggc tgaaacaccc tggcaaatgt
    10741 tgattgaaga agctcgtctg atcgcacctg gcgcggatgg tgtgaaaatg cagtgtgatt
    10801 tattgtcgtg tcagaacgct ggctggcaag gagtgacgct taataccacg cgggggcatt
    10861 tctatcgcgc ggcgctggaa gggttaactg cgcaattaca gcgcaatcta cagatgctgg
    10921 aaaaaatcgg gcactttaaa gcctatgaat tattgttagt cggtggagga agtcgcaaca
    10981 cattgtggaa tcagattaaa gccaatatgc ttgatattcc ggtaaaagtt ctcgacgatg
    11041 ccgaaacgac cgtcgcagga gctgcgctgt tcggttggta tggcgtaggg gaatttaaca
    11101 gcccggaaga agcccgtgca cagattcatt atcagtaccg ttatttctac ccgcaaactg
    11161 aacctgaatt tatagaggaa gtgtgaaatg ctgaaaacaa tttcgccgtt aatttctccc
    11221 gaactattga aagtgctggc agagatggga catggagatg aaattatttt ttccgatgct
    11281 cactttcccg cccattcgat ggaaccgcag gtgatccgcg ctgatggcct gttggtgagc
    11341 gacttgctcc aggcgattat cccgttattt gagctggaca gttatgcccc gctgctggtg
    11401 atgatggcgg cggtagaggg tgacactctc gatcctgaag tagaacgacg ttaccgtaat
    11461 gcgctttcac tacaagcccc gtgtcctgac atcatccgca tcaatcgttt tgcgttttat
    11521 gaacgggcgc aaaaagcttt tgcgatcgtt atcacaggcg aacgagcgaa gtacgggaat
    11581 attcttttaa aaaaaggggt aacaccgtaa tctcataccg gtacgcccgc atgacgcggg
    11641 cggttatcga atgatggggt gaaaaatatg aaagcggcac gccagcaagc gatagtcgac
    11701 ctgctgctga accataccag cctgaccacg gaagctctct ctgaacagct aaaggtcagt
    11761 aaagaaacca ttcgtcgcga tctcaatgaa ttacagacgc agggtaaaat tctgcgcaat
    11821 catggacgcg ctaaatatat ccaccgtcaa aatcaagaca gtggcgatcc ctttcacatc
    11881 aggctgaaaa gccattatgc gcataaagca gatatcgcgc gcgaggcgct cgcgtggatt
    11941 gaagaaggga tggtgatagc cttagacgcc agttcaactt gctggtatct ggcacgccag
    12001 ttgcctgaca tcaacattca ggtcttcacc aatagccatc cgatttgcca tgaactcggt
    12061 aaacgcgaac gcattcaact gatcagttcc ggcggcacac ttgagcgcaa atatggctgt
    12121 tacgtcaatc cctcgctgat ttcccaactt aaatcgctgg aaatcgatct gtttattttt
    12181 tcttgtgaag ggatcgatag cagcggcgca ctgtgggact ccaatgcgat caacgctgat
    12241 tacaaatcga tgctattaaa acgtgccgcg caatcgttgt tattgattga taaaagtaaa
    12301 tttaatcgtt caggggaagc ccgcatcggg catctggatg aggtaacgca cattatttct
    12361 gatgagcgcc aggttgcaac ttctttggta acagcctgac ggaaagggta gcaggccgga
    12421 gacgacgccc cggccttgcc tgttatcgct cgtcgcgacg accacccacc gcagcccaga
    12481 tgcggcggac gtgcaccgtc acttcttcgc gatcgtgata caactgccgc gcctgagtct
    12541 gagcatttat gccatgttca tcaagctgtg cctgaatata cgccagattg tgtgatactt
    12601 cttcgtagcg ttttttcatc ggcagtttga gattgaaaat ggtttcacgg caccagccat
    12661 taaccagcca ctgcgccatc aatgccgcaa ctttagccgg tttttcaacc atatcgcata
    12721 ccatccagga gatattgctg cgtgtcggac ggaatttgaa accgtcttcc cgcagccacg
    12781 tcacctgccc ggtatccatc agactttgcg ccatcgggcc gttgtcgacg gaataaaccc
    12841 acatgttgcg cttcaccagt tggtaggtcc agccgccagg gcaagcgcct aaatccaccg
    12901 cccacatccc gttcgccagg cgttcatccc actcatccgc aggaataaac acatgaaatg
    12961 cctcttccag tttgagcgtg gaacgactcg gcgcctctgc cggaaatttc aggcgcggaa
    13021 tgcccatata gaacggcgaa ttattgttgc tgtatgagta accggtatag cagcatcctg
    13081 gtgcaataaa gaatacatgc acaaccggac gcttcggcgt ttcatagttc gccagcaccc
    13141 cagcatcgcg caaggcagcg cgtagcggaa cagtgaactt acggcagaat ttcagcaact
    13201 ctttgctttc gttggtatcg gcaacttcaa cacgcagttc accgcccttc tctactacac
    13261 cctgtaacat gccgacaatg ggggtaatac gatcttctgg cggcaaatgc tgcaggagtt
    13321 cccccaccac aaaccactgg cgggcaaaaa ttaatgaact gaacggcagc tcacggatta
    13381 acttatcgcc atcatcaggt tgataacatt cataaatgac ataacccgca ttctctttca
    13441 cgcgggcaaa accgaaaatt tcccgctggc cggctttatc ggtaatttct gcggcgcact
    13501 ctttttcaaa gcccggacgg cacagcaata caaccttatt catgacttac gcccttgcgc
    13561 tttaaacgga tagcaccaac taacattaac gcccagcccg caaggaagct cacgccgcca
    13621 accggtgtga caaacgccca caaacgcaga tgggacagcg ccaggcaata aaggctgccg
    13681 ctgaacagca ccgtgcctaa cgcgaggaaa acgctactcc agtaaaacca gatactgatg
    13741 cgacgctgca ttgccaccgc cagacctaag atcgccagcg tatgaaacgc ctggtattcg
    13801 agaccggttt ggatccagcc catctcaacg gcccccatgg ttttacttaa cacatgcgca
    13861 ccaaaagcgc ccagtgccac aaaaatgaag ccgctaatag cggcgaaaat cagcataaaa
    13921 cggctggtca tggtcgtacc ctacgtaaat tattgttcat aacgaaagcg gaatttttct
    13981 tgttcagcag cggctttcgc caggatccat tggcgaaagg cggctatttt acccagttct
    14041 gcctggctgt catgacaaac cagataaaaa gcatttttac tgaccagaac atcattaaac
    14101 gggcaaacaa gacgtccggc ctcgatttca gattgcgcca tcacgttatt tgccagcgcc
    14161 actccctgcc cgtgaatagc cgcttgcagc accatggcgc tatgactaaa aattggccct
    14221 tgctgaacgt tgatatgatt aaaccccaac tgtcgggtat atgtctgcca gtcacggcgt
    14281 gacgcatcat gtaataacgt atgtttagcc agatcttccg gggtcttcaa gggtttttcg
    14341 cctgtcagca gtagcggcga acacaccggc aataaatatt cggcgtacag tttttccacc
    14401 cgtagccccg gccagttgcc ccgaccataa aatatcgcca catcaacatc atccgccagc
    14461 ttatcttcct gacgatcaac cgcctggatt cgaacgtcaa ttcccggata agctgaatta
    14521 aagctggaaa gtcgcggaac caaccaatga atggcgaaac tggggagtaa actgaccgtc
    14581 aacgccccct tggcgctacg ggcctggagt ttacgcgtcg cttcggttaa ttgcgaaaat
    14641 atctctttga tatcgaggaa atagctttgc ccttcctcgg tcagcaggag tgaacgatta
    14701 cggcggcgga acagttttag ccccaaaaaa tcctcaagag acttgatttg atgacttact
    14761 gcggcctggg tcacaaaaag ctcttctgct gcgcgagtaa aacttaaatg gcgtgctgcg
    14821 gcatcaaaaa ctcgtaaggc atttagcggt ggtaatcgtt tagacatggc ttttaaactt
    14881 tgatgttaaa tgaatttaac aattagatca cactgtgtaa cctattagtt tttttaatct
    14941 gagccattat aaattgtccg ttgagcttct accagcaaat acctatagtg gcggcacttc
    15001 ctgagccgga acgaaaagtt ttatcggaat gcgtattctg gtgaactttt ggcttacggt
    15061 tgtgatgttg tgttgttgtg tttgcaattg gtctgcgatt cagaccatgg tagcaaagct
    15121 accttttttc acttcctgta catttaccct gtctgtccat agtgattaat gtagcaccgc
    15181 ctaattgcgg tgcttttttt taccttgcga tcgcgaatta ctgttccagt tcgaccatct
    15241 ctttcacgtc agtacggttg atctgctgtt tgttgccatt agcgtcttta tacgaaatca
    15301 taccgatatc gttatcagtc tgtggtttgc cgtcagagac gatggtacgt ccgtcattgg
    15361 tgtgcatcac atagttcgaa ccggaacagg cgctcagggc aaaagtcagc atacaggcag
    15421 aaataattgc ggcagtcttt ttcatcatct tctccttaaa acgattaata aatcttaata
    15481 gtattgatta acaggctaaa attaacgcct aacactattc agcatatgtt acttacgcgg
    15541 cttcgccagg atatccagat aattctgatg gttagcactc tccttgtatc aaagtgaatt
    15601 ttgcgtcacg atcggtgcat caagccgagg agtaccatga acgtttttaa tcccgcgcag
    15661 tttcgcgccc agtttcccgc actacaggat gcgggcgtct atctcgacag cgccgcgacc
    15721 gcgcttaaac ctgaagccgt ggttgaagcc actcgacagt tttacagcct gagcgccgga
    15781 aacgtccatc gcagccagtt tgccgaagcc caacgcctga ccgcgcgtta tgaagctgca
    15841 cgagagaaag tggcgcaatt actgaatgca ccggatgata aaactatcgt ctggacgcgc
    15901 ggcaccactg aatccatcaa catggtggca caatgctatg cgcgtccgcg tctgcaaccg
    15961 ggcgatgaga ttattgtcag cgtggcagaa caccacgcta acctcgtccc ctggctgatg
    16021 gtcgcccaac aaactagagc caaagtggtg aaattgccgc ttaatgcgca gcgactgccg
    16081 gatgtcgatt tgttgccaga gctgattact ccccgtagtc ggattctggc gttgggtcag
    16141 atgtcgaacg tgactggcgg ttgcccggat ctggcgcgag cgattacctt tgctcattca
    16201 gccgggatgg tggtgatggt tgatggtgct cagggggcgg tgcatttccc cgcggatgtt
    16261 cagcaactgg atatcgattt ctatgctttc tctggtcaca aactgtatgg cccgacgggt
    16321 atcggcgtgc tgtatggcaa atcagaactg ctggaagcga tgtcgccctg gctgggtggc
    16381 ggcaaaatga ttcacgaagt gagttttgac ggtttcacca ctcaatctgc gccgtggaaa
    16441 ctggaagcag gaacgccaaa tgtcgctggc gtcataggat taagcgcggc gctggaatgg
    16501 ctggcagatt acgatatcaa ccaggccgaa agctggagcc gtagcttagc aacgcttgca
    16561 gaagaagcgc tggcgaaacg tccaggcttt cgttcattcc gctgccagga ttccagcctg
    16621 ctggcctttg attttgctgg cgttcatcac agcgatatgg tgacgctact ggcggagtac
    16681 ggtattgcct tgcgggccgg gcaacattgc gctcagccgc tactggcaga attaggcgta
    16741 accggcacac tgcgcgcctc atttgcgcca tataatacaa agagtgatgt ggatgcgctg
    16801 gtgaatgccg ttgaccgcgc gctggaatta ttggtggatt aatgacaaac ccgcaattcg
    16861 ccggacatcc gttcggcaca accgtaaccg cagaaacgtt acgcaatacc ttcgcaccgt
    16921 tgtcgcaatg ggaagataaa tatcgccagt tgatcatgct ggggaaacag cttccggctt
    16981 tgccagacga gttaaaagcg caggctaaag agattgccgg atgcgaaaac cgcgtctggc
    17041 tgggatatac cgtggctgaa aacggcaaaa tgcacttctt tggcaacagc gaagggcgca
    17101 ttgtgcgcgg cctgttggcg gtgttgttga ctgccgttga ggggaaaacc gccgccgaat
    17161 tacaggcaca gtcgccgctg gcattgtttg atgagctggg attacgtgcg cagcttagcg
    17221 cctcacgcag ccaggggtta aatgcgttaa gcgaggcgat tatcgctgcg acgaagcagg
    17281 tttaagcaca gaaccgggtc ggataagacg ttcgcgtctc atccgacctg attgtttaac
    17341 cctgacgcgc cgctttcgcc atcatcttct tcagcgcatg agaaaccgca acaaacccaa
    17401 aggtggcggt caccatcgtt gccgcgccaa atccagatgc acaatccatc cgcttcggcc
    17461 cttctgccgt ggctttcatc gcacacaccg taccgtctga ctgcgggtac accagcgctt
    17521 cagtagaaaa cacgcaatcc acgccgagct tacctttact gtttttcact acgccaaaat
    17581 cgcttttcag gcgctcgcgc aacttcgccg ccagcggatc ctgaatcgtt ttcgccagat
    17641 cggtaacctg aatctgcgtc gggtcaatct gcccacccgc gccaccggtc gtgaccagcg
    17701 ggattttatt gcgccgacaa taagcaatca gcgccgcttt gggccgtaca ctatcaatgg
    17761 catcaatcac gtacgaataa ccggcactca tatattgcgc tacgttatcc ggcgtcacga
    17821 aatcatccac caccgttaca cggcactccg ggttaatctg gcgaatgcgc tccgccatga
    17881 cttccgcttt tgccagcccg acgttatcgc gcagagcgtg aatttgccga ttggtattgg
    17941 tgacgcacac atcatccata tcgataagcg tgattgcgcc aatccccgtg cgcgccagcg
    18001 cttccgccgc ccaggaaccg acaccaccga taccaaccac acaaatatgc gcgtcagcaa
    18061 acagttgcaa cgctttttca ccatacagac gcgctgtgcc accaaaacgc tgacgccatg
    18121 catcactaat taccacagac atagcacctc agaattaaaa agggcgaggt tatccctcac
    18181 cctaaacgca taaatgcctg atgcgctacg cttatcaggc ctacataact cctgcaatat
    18241 tttggatttg catgatttgt aggccggata aggcgttcac gccgcatccg gtatgaacaa
    18301 cgcgcactta gtcaataatc aggatgtgag gttacccctc accctgtcat atccgtaaaa
    18361 acggcataca gaatatcaca tcagccgcta aagacgttac ctgcgcccgg agccgttttc
    18421 agcacccaaa cccgtccata gtggttgtac caacctgcgc ggtgtccggc ttccggcccg
    18481 atcccttgat agatatcgaa gtgttggcct ttgattgcgc ccccgacatc caacgccacc
    18541 atcagacgca attcgtactg accgttaaat ttgccgttat tatccagcag cggcacttct
    18601 gccagcaagg tagtacctgg cggaataatt gaacgatcgg aggcaacaga agcacggcca
    18661 accagcggca ccgcacttgc ccctttcacc ggtgcaaaag attgcggttt aaagaagacg
    18721 aaagacgggt tctgttccag cagctcgcga acctcggctt cactgtgtgt ttcgccccaa
    18781 tgacgaatcg cctgcatcga catatcttct tttttcactt cgccacggtc gatcagcacc
    18841 ttaccaatgc tgcgataggc atgaccgttt ttccctgcat agctgaaaaa gttaagcgga
    18901 ctgccatcac caaagtcgat atacccacta ccctgcacat ccataatgaa gttatccatc
    18961 agagagttgc tgtaagcgag gatgtattta tcgcccaatg ccccggcgta gatctccgca
    19021 cgagacggca gacgaccacg ttttggcggc atacggtaaa taggatactg gaactcgccc
    19081 tggcgggtat ggcgcgcctg aattaccggc gtgtaatagc ctgtaaactg cacgttacca
    19141 tagttgtcgg caccttccat ctgccaggca tcaatgccga actggcgcat attgcgggta
    19201 tcaccgcctg cgcgcagcca ctcttgcacc gcgttataaa cattactctg gttgccatac
    19261 agacgcggtg acgaattacg gatatggtta atttgctcgg caaaatcacc ggcgttaatc
    19321 ggcgcgccaa cggcatctgg ctggttcacc agagagaaag gctgggtaaa tttcccgtct
    19381 ttatattgct gtccgcgatc ggttggtttg gaagagcagg cggcaagcat tgccacaacc
    19441 gtgcccataa gaaggtactt tacccaacgt cctttcattg ttctcttctt caggttaaaa
    19501 aataaggcgc aacgaagata acaaaccgcc gcgtttaatg aaatgagcag cctctccctg
    19561 acgcaaattt tgcacaaaaa ataggcttta gtggtttgtt tttgttcaaa atcatgctaa
    19621 atccgtgatc ggggtaaaaa aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc
    19681 cacggacgcg gggtggagca gcctggtagc tcgtcgggct cataacccga aggtcgtcgg
    19741 ttcaaatccg gcccccgcaa ccaatcaaat ttgatgaagt aaaagcagta cggtgacgcg
    19801 gggtggagca gcttggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg
    19861 gcccccgcaa ccaattattg aacaccctaa cgggtgtttt tttgtttctg atctcccata
    19921 aaaaaagcgc cattcagcgc ctttttatca tccccttctc gccagcgttg ccccatcggc
    19981 aaaatacgct ttaatccccg caagaataga ctccgcaact tcctgctgga aagtcgccgt
    20041 tttcagttta cgctcttcct caacgttact gataaacgcc gtttcgacca gaatggaggg
    20101 aatatctggt gcctttagta cggcaaaccc ggcctgttca acttgatttt tatgcagctt
    20161 gttgattttt ccgagcttat tcagtaccgc tttaccaaac ttaaggctgt cagcaatagt
    20221 cagcgactgc accatatcga acatggtgtg gtcgacatag cggtcaccgc ttttgctcac
    20281 gccaccaatc aagtccgagg cgttctgggt ttgtgccaga tattttgccg cagtactggt
    20341 tgcgcctttg gttgagagcg caaacaccga ggaaccgctc ggctgacgac tggtaaaggc
    20401 gtcggcatgg atagagacaa acaggtcagc acgctgtttc tgggcttttg ctacgcgcac
    20461 ttgcaacgga atgaagatgt cttcattgcg cgtcatgtac accttcatat tgccctcttc
    20521 ctcgatcaga gagcgcaaac ggcgagctat ttgcaatacc acatcttttt cgcgcgtttt
    20581 gtatttcccc accgcaccgg agtcttcgcc accgtggcca gggtcaagca taatgacaat
    20641 cggacgatcg cgccctgctt tacccggttg tggaccactt tgcgctggcg gcacctgctt
    20701 ttcgaggtcg cctttgttgt aatcctccag cagcgccagc agcgggtcct gcatatcctg
    20761 tgcattggcc ggatagaggt ccatcaccag acgctcttta aacccggcga ccggcgcaag
    20821 ggcaaacagc tgcggtttta cgttttgctt taattcaaaa accatgcgta cggtttgcgg
    20881 gtcaaattgc ccgacgcgcg ccgacttgat gaacgggtcg tctgcacgaa tttgtgccgc
    20941 catccccttg agtaccgagt tcaggtttac atcttcgata tccaccacca cgcgttcagg
    21001 gttactcaac gcgaactgct tatatttcag ctgacgattt gattctaccg tcacgcgggt
    21061 gtagctggac gcaggccaga cgcgcaccgc cacgacctgg ctaaccgcag ccaggctgac
    21121 ctgacttacg ctcaatagcc acatagcgcc cgcgccttgc agtaaacgac gacggctgat
    21181 tgcagtgttg gatcctgaca tgcctctccc gagcaaaaga aatctaagct gtgtaacaag
    21241 taaacgacta atttgaccgg aaactttagc gaaagacgca taatctgtca tctaataaac
    21301 ggtaaacatt ctttttatat tcacggcatt actgataaaa aagtcgctct cgcataaaat
    21361 ttacacttgc accctgcgaa aaaacagaat aaaaatacaa taatttcaaa taatcatgca
    21421 aaggggtgtg ccgtggtaaa ggaacgtaaa accgagctgg tcgagggatt ccgccattcg
    21481 gttccctata tcaataccca ccggggaaaa acgtttgtca tcatgctcgg cggtgaagcc
    21541 attgagcatg agaatttctc cagtatcgtt aatgatatcg ggttgttgca cagcctcggc
    21601 atccgtctgg tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct ggctgcgcat
    21661 caccacgaac cgctgtatca caagaatata cgtgtgaccg acgccaaaac actggaactg
    21721 gtgaagcagg ctgcgggaac attgcaactg gatattactg ctcgcctgtc aatgagtctc
    21781 aataacacgc cgctgcaggg cgcgcatatc aacgtcgtta gtggcaattt tattattgcc
    21841 cagccgctgg gcgtcgatga cggcgtggat tactgccata gcgggcgtat ccggcggatt
    21901 gatgaagacg cgctccatcg tcaactggaa agcggtgcaa tagtgctaat ggggccggtc
    21961 gctgtttcag tcactggcga gagctttaat ctgacctcgg aagagattgc cactcaacta
    22021 gccatcaaac tgaaagctga aaagatgatt ggtttttgct cttcccaggg cgttactaat
    22081 gacgacggtg atattgtctc cgaacttttc cctaacgaag cgcaagcacg ggtagaagcc
    22141 caggaagaga aaggcgatta caactccggt acggtgcgct ttttgcgtgg cgcagtgaaa
    22201 gcctgccgca gcggcgtgcg ccgctgtcat ttaatcagtt atcaggaaga tggcgcgctg
    22261 ttgcaagagt tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga aagcgccgag
    22321 cagattcgtc gcgcaacaat caacgatatt ggcggtattc tggagttgat tcgcccgctg
    22381 gagcagcaag gtattctggt gcgccgttct cgcgagcagc tggagatgga aatcgacaaa
    22441 ttcaccatta ttcagcgcga taacacgact attgcctgcg ccgcgctcta tccgttcccg
    22501 gaagagaaga ttggggaaat ggcctgtgtg gcagttcacc cggattaccg cagttcatca
    22561 cggggcgagg ttctgctgga acgcattgcc gctcaggcga agcagagcgg cttaagcaaa
    22621 ttgtttgtgc tgaccacgcg cagtcttcac tggttccagg aacgtggatt taccccggtg
    22681 gatattgatt tactgcccga gagcaaaaag cagttgtaca actaccagcg taaatccaaa
    22741 gtgttgatgg cggatttagg gtaagcgatg aaaatcaccg gatgcggcag gcgtaacact
    22801 cgtgcgtcgc atccggcaat tacgtttatt cccgcgaact aaacaacgcc gccagaccac
    22861 tgcgccgctc agtacgagtg gcgattgccg cacttaatat acgctcatcg gcatacagcg
    22921 acagacgacg acgcgcgcgg gtcaccgcgg tgtataccag ctctcgcgtt actaccggcg
    22981 tgcgttggct cggtaaaatc aacgccgcat ggtcgaactc cgatccctgc gatttatgta
    23041 ccgtcatcgc ccacgtagtt tcgtgctctg gcaagcgact cggttgcaca gacttaatat
    23101 tgccgtccgg catcgcaaac cagacgcgcg tcccctgccc gcgatccagc gcaataccga
    23161 tatcgccatt aaacaaccca agcgcgctgt cattacgggc aatcatcacc ggtcggcctt
    23221 cgtaccaacg agagtgcgga tgacgatgaa ttttgcgctt ctgttgcata aactgttcaa
    23281 ttcgctcatt cagtccagcc acgccaaacg gcccttcccg cagggcgcac aaaagctggt
    23341 actcattgaa cgcctgaatg attaaatccg gctcggcacg cgcttgcagc agatccagat
    23401 aacgtccgta acccgcaaga gcttcctcaa gcatcgcaat ataatcttcg ccgctctgta
    23461 aaagtcgttt ttcgatatca gtaaaatcct gctgaaaaac ggttttcact gccgttttat
    23521 caccacggtt gatcgccgca gctaactgac caatgccaga atcgctgccg aaacgatagc
    23581 ttttttgcag caggcagaga ctgtcgcgca aagatgccgc ttctgtgccg gttcctgccg
    23641 gaacgtgagt ccccgtcagg cggcttagct gcctggcacg ctcggcggta aagcccgcgt
    23701 tggcataagc gcagatatcg cccagcacag ccccagcctc aaccgaggcc agttgatcac
    23761 gatcgccgag aaagatcact cgcgcatgat ctggcaaggc gtcgatcagt ctcgacatca
    23821 taggcagatc gatcattgac gcttcatcta ccaccagcac atcaagatgc agcgggttac
    23881 cggcatgatg acgtaaacgc tggctacccg gctgcgcgcc cagcaatcgg tgcaaagtgc
    23941 tggcatcttc cggaatgcgt ttcttttgtt catcggtcag cggtaactgt cgcaaagcct
    24001 tgccgagaga ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc agacggatac
    24061 ggcagcgttc gccgtcggcc atttgaatta acgctgccag caacttcgcc acggtggtcg
    24121 ttttaccggt gcctgggccg ccggaaatca ccgagatccg ccgcgtcagc gccactgccg
    24181 ctgcaacttt ttgccagtta atttcatcgc ttactggaaa aagtttgtcc agggtttgcg
    24241 ccagtagagc ttcatcaacc tcaatggtat gattcacctc attgaagaag cgcgctaccg
    24301 tccgctcgtt acaccacatg cgattcaaat aaagacgatc gccacagagg atcatcggcg
    24361 tgggttcatc tcccctgctg accgcttgag aagccagcaa gcattcttcc caattttgta
    24421 gctcaccgat ttcactgaca caggtcgcca acagcggatt cgacgcctcg ttattttcca
    24481 gtcgtgaaag cggcaaacag acgtgtccct ctccggcatc atgacttaac agtgccgccg
    24541 cgagggtgac ggcaggatgt tcatctcccg ccacggtcag ggcaaattgc acatccagcg
    24601 ggcgtagctg tttgtgctcc acagcttcca gtaattgctt ttgcaatttc attacgcctc
    24661 ctccagggtc ataccggcaa acatctcatc catcagggca atcaacccgg cgttgggtcg
    24721 ggttgtgtaa atcccttgtt gcggatgttc tttatcaacg ccacgcagga acagatagat
    24781 aacgccgcca aagtgaagat catagtcgta atcagcaatg cgatggcgca gataacgatg
    24841 cagcgccagg gtataaagct gatattgcag atcatagcgg tgtgcctgca tcgctgctgc
    24901 catagcctgt tgggtgtaag ccgaactgtc ttcacccagc cagttggatt tatagtcgag
    24961 caggtaataa cgcccttcgt ggcggaatac caggtcgata aagcctttta acatgccgcg
    25021 tacctgcatg aactccagcg gcgggcagcc agcggatagc gggtcaaact ggcgaattag
    25081 tgcatcaagc tggctggcga tgagcggttc gctaatcggc aggtaaaact ccatctccac
    25141 ctgtttatcg cggtcggaaa gctgactcag gctaacaccc gtttcattga gaggtgcctg
    25201 gaggacagcc gtgatccact cggtcaacac cggttcccac tgcgattcaa agccgccgag
    25261 ttccagtttt tcctgtaccc agttcgggtc aaccggctgg gtaaaatcga ggtcttcaaa
    25321 caaactgtgc aagaatgtcc ccggtgacgc accgcgcggg aactgatgcg gtgttaacgt
    25381 cggttcttca acgacgctga ccacgcctgc ggcatcaaca tccagccgtg gcatcagatc
    25441 ctgagcgata ccgtgaccac gctgctgcaa gccggagtag ctggtgacac gccagttatc
    25501 gccgggcaat cgttgtagtg tcctcgcatt cagttctgca gttaaagcat cattaacctg
    25561 ccagggctgg ttatcaccag tttgtgccgt ttgccaggca atatcatcat cgcataacgc
    25621 ttcaatacag gtgcgaagcc ctgccgcatc ttgcggttcc cctttttgca gcaaacgccc
    25681 gagcgcactt tggtggacgt cggtgtcacc ttttttatcg ccacgacggc gcaccagcgg
    25741 tgcaacgccg agactgcaat gccaaaccga acgcgtcagc gccacgtaaa gcaaacgcag
    25801 atcttccgcc agacgttcga cctccgcgag gtcgacgctt tctggcgcag cattaagatc
    25861 caaaactgcc tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga cgcggaaatt
    25921 ggtaataaat ggcaaccaga ccagcggata ttccagccct ttcgatttat ggatcgtgac
    25981 aatctgcacc agatgtttat cactttcgag gcgcatttgt tggctggagg cattactgtc
    26041 tggctcgagg atatgttgcg ataaccagcg taccagcgca tgttcacttt ccagctgcgt
    26101 tccggcttct tgaagcagtt cgctgatatg caagatatcg gtaagacgcc gctcaccgcc
    26161 tgccgttgcc agcaagtttt ctgcaatgtt acgcgccgac atcagcgccc gcagcatcgg
    26221 cataacgcca cgtttgcgcc agatttgccg ataaccatcg aactcttcga ctaccgcatc
    26281 ccacgcatgt tcgtcattat tcagcgtttc aatatccagc gcattcagcc ccatcattga
    26341 cgttgccagc gcactgcgca gggtgttctc acgttcgggc gtcatcaccg cctgcaacaa
    26401 ccaaagcatt tcctgcgctt ccagagtttc aaaaacactg tcgcggttcg aaaggtaaac
    26461 ggaagggatt tccagcaacg ttaaggcatc gcgcacctgg gcggcctcct ggcggctgcg
    26521 caccagcacg ctgatgtccg aagcacgcac cggacgcgcg tcgtcgccgt tcatcagcaa
    26581 cgcttcgccc cgctgtccgg cttgtagcca gtcgcggatt tgcgcagcac atacctgcgc
    26641 catggtactt tgatagtcgc caacgccgca gctttcgcct tccatcagcc acattttcat
    26701 cgcaggctgc gtttcacctt taaatacaaa acgtaacgcc tgatttttcc cggctgattt
    26761 cactggaata aacggtattt cgcgaaacat gaacgtgtca tcagtctggc tgaaaagctt
    26821 gttcacactg ttcaccattc caggtgcgga gcgccagttg gtgtctaaag tgtagtgggc
    26881 gtgaacttca ctacgcgcct tcatataagt gaagatatcc gcaccccgga atgcatatat
    26941 ggcctgcttc gggtcgccaa tcagcaacaa tgcggtttcc ggctgatggt gccagatacg
    27001 gcgaaaaatt cggtactgct gggggtcggt atcctgaaat tcatcgatca ttgccaccgg
    27061 gaatcgcgta cggatcgccg ctgccaacac ctcgccgctt tcgctacgca gcgcggaatc
    27121 gagccgactt aacatgtcat caaaacccaa ttcgccacgg cggcgttttt cacgcgctac
    27181 tgtttcgcgg atctcagcca atgcgcgggt gatcaacaga tcgcggatcg acaatggttc
    27241 tgcaagcagt tgatcgatcg cctcaaacag tggatgtcgc ggggtttccc ccccggcctt
    27301 cgtgcgatct tctaagaaac gctgggagaa tttttccagc gactccggca actgataact
    27361 gtttgtctct tcttctgccc aggcgctgat cttttcgatc catttagcct gattgctacg
    27421 gttaaacttg cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt cacccactgc
    27481 gtcgcgccac tgctgtttta ccgtatcaat acgcgccaca atttgcgcgt ggcgggaagc
    27541 cagcgtttca tcatcgggcg gcggtgcttt gataaccggc ggttcgcctt gcagataacg
    27601 attaatatcg cgcagcaacg cctgcggccc tttccaggtt tcaaagacga cctgggctat
    27661 ttcacgcggc agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct ggtagcgtag
    27721 cagagactca tcttcaatca gctgctgctc aaacagcatg ccggattcaa aggcattcag
    27781 gttgagcatg cgctggcaaa agccgtgaat agtaaagact gccgcttcat ccatctgccg
    27841 ttcggctaac aacaaccact gcgcggcttg cgctttatcg tcgatctctt ccagcaggcg
    27901 ttcgtacagt ggattgtcgg tggtttcacg cagacaggcg atgcgcaact cgtggatatt
    27961 gctacggata cgaccgcgca attctgccgt ggcagcctcg gtaaaggtga ccaccagcag
    28021 ttcttcaacg atcagcgggc ggggaaaggc ggcggaaccg cctagtccaa gtaacaggcg
    28081 caaatagagc gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt caatcaggcg
    28141 ctcgccctgt aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac tcattctttt
    28201 cactcatcag gggcattgtt tgctgcaacg cgctgacgtt ctcccacact ttccagcctt
    28261 caggatgcac atattcggct ttcccgttct ggctgccgga aatctgcgac agaatagcca
    28321 tgccttgcgg ctcgaccacc gcctgatgga agaaatcagc aagtttttgc ggcgtcagca
    28381 gttttatctg ggccacgatt ttatcacgcg aatcgaagcg catattgccg cgatcgaaat
    28441 ctttacttaa cttcgatgct tcttcgccga gcgtttgcgg tgcctgcagc atctgggtaa
    28501 ttaccgcctg ctggatttgc gcaaactcat ctggcttcat cgctcgcaat tttgcctctg
    28561 cggttgggaa aaacgccttg taacgctccc acaagaatga aggctgttta tcattgcttt
    28621 gcaaaaggaa gcccatgccc cactgacgcc ccacgctcat tggaaacgca aacacggcat
    28681 agcccaattg ttcttcggta cgcaactgat tgtagaacca cggctgtacg atctgtccca
    28741 acagagagct ataggctgag ctggtgtatt catcgtagcc agtcggtaca aataccgctg
    28801 ccagtgcgga gtcggtgctg ttaccggctt tttcaaagat gacggattgt tttttatcga
    28861 ccactacatc tttgtttcga caccactctg aaccatcagc gcccaactgt ttttgcacat
    28921 cgcgtgccag cgttgttgcc tgggcctcgg tcatgttgcc gataaccata aactctggtc
    28981 gagcccctga ttttaaggcg tcgcgatagg ccagcacctc tttcaacgta atggagggca
    29041 aaattttacg ccgttcatct cgcgagaagt acggcacttg cgagagcatc tgcgcgggca
    29101 taatcgcctg ctcaaacgct ttaccctttt ctgcggaatc catcatctgg ttataccagg
    29161 acttcgcctg ctcaagctgc tcttccgtag cggtatagct aaagtacccc tcgagcaatg
    29221 cctggaacag ctgcggcaga cgctgggtgt aaccattagc attaatcata aggccgttgt
    29281 tagcgttggt ggaaaaactt atgccaccaa ccgacgcctg gttgcttaac tgatcaagcg
    29341 ccagccctgc gagataatca ttgagcgcaa acatcacctg attgcgggcg ctgtccatgg
    29401 ctttcggatt acgcaaaatc aggctgacat cagctttggg ctcgctggca aaataacggc
    29461 ttggcgcata caccacgcgc agattcgact catcaacaat cagctctgga tggtcgtatt
    29521 tcttctctga cttaatcagc gagaaatcat caggaatata agggttaagc tctggcaaag
    29581 agagcgcaat gtcggcggct tttttctgcc agtcggcgaa agtttgtgcg ctgattttat
    29641 cgacctgata cggcgcatcg acaaagtaag ccgttttgtt gtgcggctct ttcgggctga
    29701 tataccagat acgcgcattc tgcggcgtca tcatcgccag acgttccttt actgctttag
    29761 catcgtaccg atcggcaata ttgactgcat ccagcgtatg ctcaacagga acgcgaatca
    29821 tggtatctgc cagccattcg acgtaatcca tatcacgggt gatcgacgga taacggaagt
    29881 cgatatccag cacattcgcc agttcatcga agtattgttt atcaatgcct ttttcacgta
    29941 acagattgag atagctaaaa attgccgcca caacctgatc gcgattagcc aagcctttat
    30001 cagttaaaga cgcagagatc gctaatacgc cgctgttgcc gttgacgata ggatcggagt
    30061 tggcgctaat gccctcaact aatccctgct tttgcagcca gtcagaaagt gtacctgggc
    30121 tgcgattgcc aatcagatag gtaatcaatt catcggtttt actacggaac ttcgctgagt
    30181 tgttatcgat gcgaaactca acgcgcaaca ctttacgcgg cagcgcaggg acgtaatgaa
    30241 tgataatgcc cttttgcgcg tcggtgacta ccggcacggt gatttccggt tttttgctct
    30301 ctttgttcgg cacgcgacca aaggtgtccg ccgccatttt tgccaactcc ggcagcggtt
    30361 tattactgta aataaccgcc ttcatcaaat tggcggaata gtacttctcg tggaaatctt
    30421 tcagcgcctg ctgcaccgga ttaccaggtt tgtcgcttaa agtttcgagg ttaccaccag
    30481 aaaactttga accggggtgt gccgggttaa tggtttctgc gctgacctgt gccatgcgca
    30541 tcccgtcacg cgtacgcgcc atggttaatt cagcgttcac tgcattacgc tcacgttcgg
    30601 cgtatttctt gtcgagcaag ggttctgcaa tagcatcggc caggcggtct accgcaccgg
    30661 gcaaggcgtc gttctcaact tccagataga aagccgtgcg atacggcgca gtgctggcat
    30721 tgtgactacc gccgtgcatt ttgagatatt cggccagact gtcagcctgc gggtactttt
    30781 tcgaccccat cagactcata tgttcaaggt aatgtgccag cccctggtac gcctcgggat
    30841 cttccagcga cccaacgggc actaccagcg ccgagagcga tttaactgcc tgcggatcag
    30901 aaaccagcaa gaccaccata ccgttatcca gacgtatagc ctgatactgg cggttatctt
    30961 tatcactttt acggatggtt tcctgaatcg gctgccatcc cgtttctgcc tgacttaatg
    31021 gtgcccagag ggcaacaaac aacaataatg ctttgaacca ggtgctgcgg ggcattcacg
    31081 gacctcataa tcaactttat tttctggaca gattcaacag gttaacgcaa atcatctgcc
    31141 agaatttaat cttgtgctgc acgagtcagc ctatgtttat ataaccatca gtccgtgact
    31201 ggtgcgcatc ataaagtaag cggatagatt gcgcaatttt tatacaacac tcatgactga
    31261 ttaaagcgaa acagcggtaa caggaaacgt tgcgactgtt caacgatggc ctccattgtc
    31321 tctggtgtta attgccgcca gagccgttga taccagatat catcaccttc gccacgcacc
    31381 atcatgttgc cttcgtaagc ctgaaggaat ttcgtacggg ctttttgcaa cgtggaatcg
    31441 tcatccagca tggcatcgtt ttgcgcgtca taacaggttt ttagccacgc gccgccactt
    31501 tcaggtaaca ccagcaatgg cgcggacatt ccttcacgat acccctcaat cagttgtgag
    31561 aggtaatgca aagcctgttc ggctgcaagc ggcggaaaac gccactcacc gtctttgcgt
    31621 agaaaaaggc gactttcgcc attaccaccg ctggcacagt agacaaggtg ttccagccaa
    31681 agttgcattc cctgcgccac acttaataaa gagggacgcc agcgcaacag gccatccggc
    31741 tgcacctgcg gcaaccagcc agttatctgc acaccgttgc aggcgagatc aatttccata
    31801 ctctgccccg gctggcgaca ggcaatgact ctgtcggcaa gctgctgcat ctcctggcac
    31861 tgtgtttccc agaaaatttc accaaaagcg ccatacggta aatcccctgc cgcccggaag
    31921 cggcggaaca agcgttcggc atcatcctgc tcaaccagtg cattcaataa ctgctgattg
    31981 atttgataac ggctaagtcc ttccagaata aatggctcgg tgtctgggat ttcgctgtct
    32041 tcagtatgaa agttcacctg caaacgcatc tggaagaacg cccgcaccgg atgtgcccag
    32101 aatcgttgta gcgtttccag cggcacggtt tccggtaagg taaacggcag cggctgaaca
    32161 aattcagaat gtgctttacc agcctggctg gccgcaggta gccattcacg agcatagctt
    32221 tgtcgttcgc ctggctggta gttttgcgga tcaaacggca tccgggtatg gaggcaagta
    32281 agatgcgctt ttacccttgc ctcgctttca tcacagttga gcgcttcatc gcccggtaga
    32341 taatgacttt gcccgatgta gtcgatcagt tcctgcacca gtaccgacgg gaaacgttca
    32401 ctgttatcct gaatggaacg cccgatatag ctgatataga gtttttgctg cgcggaaatt
    32461 aacgcttcca ggaacaggta gcggtcgtca tcgcgacggc tacggtcgcc acgcttcggt
    32521 ttctggctca tcaggtcaaa gcccaatggc gcaagctgac gtggataaac gccgtcgttc
    32581 attcccagca ggcaaaccac tttgaacgga attgaacgca ttggcatcag agtacaaatg
    32641 ttaaccggtc cggcgagaaa acgctggctg atacgttctt gatccagacg ctgtgccagt
    32701 tcatcacgca atagtgacag cggcaccgcg tcgccatact gcgcacctaa accttcggcg
    32761 ataatcgcct gccattgttg ttcgatcagc gtcatcgccg cttcggtttc cgcatccggc
    32821 aggaagaagg cgttgagcat atcgcgacaa accggcaacc actcttctag cggacgctcc
    32881 tgtgccagcc cgcgacgcca gatgtttagc tgcattagca gtgaagccag atgccccacc
    32941 agttctgcaa ttaagccgct cgattcatca taaggtagaa ccgattgcca ctcgccctgc
    33001 gcgctctcca tcgcgtagcc cagcaacata cgcgtcaggc caaatcgcca ggtgtgttgt
    33061 ccggtggcgg ggagttccag ctcgcgaacg ttgtcgtcat ctatccccca acgaatgccg
    33121 gattcgttga cccactggcg taaataacgc agcccttctt cggtgatgtc aaaccgcgcc
    33181 gccagcaccg gcacatccag cagcgccagc acatcctctg acacaaagcg actgtcaggc
    33241 agtgataaca ggctgataaa cgcttccagt accggatgtg actgccgcgc acgacggtcg
    33301 gaaatggcgt aaggcaggta acgatccgca ggtgcgctac caaacactgc ctgaataaac
    33361 ggactgtagc tgtcgatatc agccaccatc acgatgatgt cgcgcggagt aagtgtcggg
    33421 gcttcctcca gcatcgccag caagcgatcg tgtaaaactt caacttcacg ctgcgggcta
    33481 tggcaaacgt ggaatgtgat actgctatcc agtggatcaa gcgggcgttt gttatcgcta
    33541 cgggaaaact cttcgatgtt cacacccgca acggcgcggt tttccagttc cagaatgtca
    33601 gactgaatgt tatgcagcag gttatctggc gtcacatcga caaaagcgtc cagctcctgg
    33661 ctgctctcca ggtcagaaag gagataaatg tagtcgcgcc caagcttccc ccatgaggcc
    33721 agcagcgagt tgccgacatc ctgttcacca tcgctgttaa agagctgtcc ggcattttcg
    33781 ctatcgcgaa atagcggcaa ttcgcgatct tcaaaactgt gtcgacgctg acgagtcagc
    33841 agtttcgcca gataagcagg atctttaata tcgccccagt aataacggca ggggttggta
    33901 aacaggagat ggatttcaat atgtttaccc agcgcctgga gcgcctggag ataaacaggc
    33961 ggtaacgcgg aaataccgca tataaagacg cgcgaaggta accccggcgg gcaggtcgtc
    34021 gcggactcca gcgtttcgat aaagcgctga tagagattcg cgcggtgcca gcgcggttgt
    34081 ccgagttcat gggtatattc caccagcgcc ttccacaacg gggcttgcca ggcctgtgct
    34141 tctcccagcc cttcaaccaa atgtcctgtt tcccactgtg ccagccagtc cggacgatag
    34201 accagatact ggtcaaacag gtccgccgct tttgaggaaa gctggaacag ttttcgcttg
    34261 tcgctatcgt cagtcagata atgccgcaac agggtaaagt cttcgcgctc cagcaattgc
    34321 ggcagcagag tcatcagttt ccagctcatg ctctgtttgt taaaggcgct ctctttgggg
    34381 atttccggta acacccggac gaacatatcc cagataaagc tcgctggcag cggaaaatca
    34441 atgtttgccg caataccaaa cttttgcgac agggtcattt gcagccactg cgccataccg
    34501 gtactttgca ccagaatcat ctctggttcg aaaggatcgt ccagccgttc gcgttcgaca
    34561 ataaactcca tcaacgcttc cagcacgtcc agacgattgg aatggtagac ccttaacata
    34621 gcggctcctg actactgacg attcgggcaa tgcaggcggg tcatctcgcc ttctctgccc
    34681 ccgggtgaaa ctagcgtaac gctgatgctg acacatcccg cctgcgatgt ctgcattcgg
    34741 ttgacctgcc agttggcagg tggcgaaatc gcgcgcagtt gcgtttgctg ccaaccatgc
    34801 cgccagagct gttggtactg gtttctgctg gcgagactgt tcattgatgt tcgctggata
    34861 cccgataacg cagtgacaat catcaccatc agcaccatca gcaccatcgc aaccattacc
    34921 tccggcaggc taaagccttg ttgattcttc agggaagctg acataatgcc ccctctttca
    34981 gcggacaaaa atcgctccag ccgcgtggcg aaaaaacaat ttttccatca atgacttcgc
    35041 ctgttcgcca caacgaaacg ccttcataac cggcaatcaa taaggcttca ttatctgcca
    35101 gtaaacgcaa acaaacctgg gcatcggttc cagcgtactg cgagcactga actgctggct
    35161 gcgtctgcca ggagtgcatt tttccccacg ccagcgccga ctgaacgatg gcctggcggc
    35221 gcaatgactg actttccatg ctcacgcgag aggcaaaact gcgatcctgc tgactcattc
    35281 cttgcaatag caagctaccc aaaaccagca gcatcaggac cagagccagt gacgaaacac
    35341 ctttttcgcg gttcacaggt tgaatcctgt cacgctatag ctggcatcca ccacggtttg
    35401 cggttcagac ttactggcag cacgcatatt aaccgtcaac accggcgaga agccgctgac
    35461 atcctgacgt acgacctgaa aagtgtcgat aatgatggca tccggattag tgactttatc
    35521 ccagccctta ccttcacagg atgtcgcacc gcgtagcgtt tccagcacat gctccttcag
    35581 acgaaatcca atctggtcgg actcttttac cggttcgcga tcccagatac cgttactgtt
    35641 cgcatcccac tgcacaatga tacagtcacc ctgtccgaca atttccagcc cttcgccggt
    35701 acagctgcca tgacaataac ccgccctctg gagatgcttc gcgacggtaa atacccgcag
    35761 ccagatttca tcttccagtg ccagcttacg agtgctcgtt aaactttcac gctgtaacgc
    35821 aggcagaaag cgtgcagccc ccagcaacaa tacgctactg atcgccatag caatcaacac
    35881 ttccagcaga gaaaaacctt gctcttttac aggcatcctt ctgtttctcc ttgctgataa
    35941 agccggagtc ttccccacgg cgaaaccacc agccaccact cgcccgttga gtttttgaag
    36001 cgaatatgcc cggcccatgc ggtattacgc aggccaaaga aagcaagcga aggtgtcagg
    36061 tcgctcattt cgacttcggg ccagcgtggc acaaagacca atggtgaact gccatgacag
    36121 gtattggccc cagcagcgga actcacaagg caccataacg tcccctccct gataacgctg
    36181 atactgtggt cgcggttatg ccagttggca tcttcacgta aatagagcaa atagtcccgc
    36241 gcctagctgg cggtttgcca aagccgttgc gactgctgcc agtattgcca gccatagagt
    36301 ccacttgcgc ttagcatgac caaaatcagc atcgcgacca gcgtttcaat cagcgtataa
    36361 ccacgttgtg ttttcatgcc ggcagtatgg agcgaggaga aaaaaagacg agggccagtt
    36421 tctatttctt cggcgcatct tccggactat ttacgccgtt gcaggacgtt gcaaaatttc
    36481 gggaaggcgt ctcgaagaat ttaacggagg gtaaaaaaac cgacgcacac tggcgtcggc
    36541 tctggcagga tgtttcgtaa ttagatagcc accggcgctt taacgcccgg atgtggatcg
    36601 tagccttcaa tctcaaagtc ttcgaaacgg tagtcgaaga tggattcggg tttacgtttg
    36661 ataatcaact tcggcagcgg acgcggttcg cggcttaatt gcagatgagt ttgatccata
    36721 tggttgctgt acaggtgcgt atcgccaccg gtccagacaa aatcacccac ttccagatcg
    36781 cactgctgcg ccatcatatg caccagtaac gcgtagctgg caatgttgaa cggcaggccg
    36841 aggaagacgt cacaggagcg ctgataaagc tggcaagaga gtttgccgtc tgccacatag
    36901 aactggaaga atgcatggca cggtgccagc gccattttat ccagttcgcc tacgttccac
    36961 gctgaaacaa taatgcggcg cgaatccggg tcgtttttca gctggttcag taccgtagta
    37021 atctggtcaa tatgacgacc atctggcgtt ggccaggcgc gccactgttt accatacact
    37081 ggcccgaggt cgccattttc atcggcccat tcgtcccaga tggtgacatt gttttcgtgt
    37141 agataagcaa tgttagtatc gccctgcagg aaccacagca gttcgtggat gatggaacgc
    37201 aggtggcaac gtttggttgt caccagcggg aatccatcct gcaggttaaa acgcatctga
    37261 tgaccaaaaa tggaaagcgt tccggttccg gtacggtcgt ttttctgtgt gccttcgtcg
    37321 agcacttttt gcatcagttc taaatactgt ttcatggttc ctcaggaaac gtgttgctgt
    37381 gggctgcgac gatatgccca gaccatcatg atcacacccg cgacaatcat cgggatggaa
    37441 agaatttgcc ccatgctgat gtactgcacc caggcaccgg taaactgcgc gtcgggctgg
    37501 cggaaaaact caacaatgat gcgaaacgcg ccgtagccaa tcaggaacaa gcctgagaca
    37561 gctcccattg ggcgcggttt acgaatatac aggttgagga taataaacag caccacacct
    37621 tccagcagca gttcgtaaag ctgtgatggg tggcgcggca gcacaccgta agtgtcgaaa
    37681 atggattgcc actgcgggtt ggtttgcagc agcaaaatat cttctgtacg ggagcctggg
    37741 aacagcatgg caaacgggaa gttcgggtca acgcggcccc acaattcacc gttaataaag
    37801 ttgcccagac gcccggcacc aagaccaaac ggaatgagtg gtgcgataaa atcagagacc
    37861 tggaagaagg aacgtttagt acggcgggcg aagataatca tcaccacgat aacgccaatc
    37921 aggccgccgt ggaaagacat gccgccgtcc cagacacgga acagatacag cggatcggcc
    37981 ataaactgcg ggaaattgta gaacagaaca taaccaatac gtcccccgag gaagacgccg
    38041 aggaagcccg catagagtaa gttttcaact tcatttttgg tccagccact gcccggacga
    38101 ttcgcccgac gtgttgccag ccacattgca aaaatgaaac ccaccagata catcaggccg
    38161 taccagtgaa gcgccacggg tcctattgag aaaatgaccg gatcaaactc cggaaaatgc
    38221 agatagctac tggtcatctg tcaccacaag ttcttgttat ttcgctgaaa gagaacagcg
    38281 attgaaatgc gcgccgcagg tttcaggcgc tccaaaggtg cgaataatag cacaagggga
    38341 cctggctggt tgccggatac cgttaaaaga tatgtatatg atccgcgcta taaccctccg
    38401 cgaatcagcc cgcccatgcc gcgacgctcc ataaaggctg caacctgatg acgaacttcg
    38461 gtcgccagtt gcgcttccag actacgctgc gcaagatttt ctgcttcggc aaaatcaatg
    38521 cgccgcagca ggtattttac ccgcgctaca gaacgtccgt tcatagacag atggcgatac
    38581 ccaagcccaa tgaggattgc cacgcacatg ggatcgcccg ccatttcacc gcacaaacga
    38641 agatcgatac catgtatttc cgcttcccgg gcgatcatcg ccagagctcg taacattgca
    38701 ggatgaagac tgtcgtaaat gttcgccacc cgggtattgt tgcgatcaac ggccagaatg
    38761 tattgagtca gatcgttggt gccaacagag atgaaatcga cccgctttgc cagatgcggc
    38821 agcataaata ccattgacgg cacttccagc atgatgccga tacgtggttt gggaatttcg
    38881 taaccgatca tctcctcgac ttcacgtccg gcacgttcaa tcaggcggcg tgcttcgtca
    38941 acttcatcga ggctggtgac catcggcaac agaatattca ggttgcccgt agcggcatta
    39001 gcacgcagca tcgcccgcac ctggatcaag aagatctccg gctgatcgag cgtaatgcga
    39061 atcccacgcc aacccaggca tggattctct tcgctgatcg gcatgtaagg cagctgctta
    39121 tctgctccga catccagcgt acgcaaggtg acgggtttat cattgaacat ttgcagcatc
    39181 ccctgatact gcgccacctg ttcttcttcc gacgggaaac cactttgcag catgaatggg
    39241 atttcagtgc gataaagacc gatgccatca atacagctgc ccagtttttc ttcatgttcc
    39301 gggcttaaac cagcattgag catgactttt atacgctcac cgctttttaa ctgcgcgggt
    39361 aaattgacgt catcttccgc cagacggcta agctcaatct cttcactaat tagccgctga
    39421 tattcttgca gcagtaccgg ctccggatcg accagcaatt caccgcgata gccatcaacg
    39481 atcagcgtcc tacgatgcag caccgaaggc tgaatatccg cgcccatcac ggtagggatc
    39541 cccagcgcac gtaccatgat cgcagcatgg gagttggctg agccatctcg cacgacaaca
    39601 ccgactaagc gatcctgggg cagctcagca agagttgtcg ctgacagttc atctgccacc
    39661 agaatgaaac gttccggcca ggcgttcggc ccttgattag cgtcatcaag atgaaacagc
    39721 aatcgctgac ccagcgcacg taaatcgcca gcccgctctt tgagatagtt atcgcttagc
    39781 gcggcaaact gttcggcaaa tttttcaatg accgttttta ccgcccactc tgccaccgag
    39841 cctttatcaa cctcggcaaa caattcgcga cgcagccggg tgtccgaaag caggtgcgag
    39901 taaagatcga aaatagccgc cgtttctttt tgtgctccgg cggcaaagcg tttgctgtag
    39961 cggcgaaact cgtttgccgc ttcttccagc gccccggtca gtcgttcgcg ttccagagcc
    40021 ggatccagcg ttgatgcctg atacacctgt tccattaaag gtaacgtggc atcctgccag
    40081 ccttcggcaa tcgccacacc aggtgctgcc ggtaatgcgc ggattcgcgt ctggcgatat
    40141 tgcccaaaca gggcagtcaa ctgcgactga gaaagaatag ctgccatctg ggtggcaagc
    40201 gtcaccagga aggattcttc actttcgtca tactggcgca actctcgttg ctgtaccacc
    40261 agtacaccaa gcaactggcg acgttgaata attggtacgc ctaaaaacgc gcggaaacgt
    40321 tcttctttta cggaggggat gtatttgaag ctggggtgct tttgcgcatc tgcaaggttt
    40381 atcggttccg ccagcctgcc aaccaggccg acgatccctt catcaaacgc gagcgttaca
    40441 gtgcgaccgc gtggtttttt cagcccccgg gtcgccatca ggtagtaaca acgtcgatca
    40501 tgatcggcca ggtagaccga acagacctcg gtatccatcg caagacagat gtcggtaacc
    40561 agaatattta acgcctcatt caggcgtggt gcgctggcta ccttttcgac tatttcgcgc
    40621 aggcgagtga gcataattgg cgtgacttaa cctcttttac gtcgataagc agatgcgttt
    40681 tgtggttttg gcgtattttc ctgcagtgac atcaccacac tcgcgaactc tttcattacc
    40741 ctacggtaga catcacgttt aaatgacacc acctgtctga ccggatacca gtaacttacc
    40801 catcgccagc cgtcaaactc tggtgtactg ctggtttgca tattgatttc tgcatcgccg
    40861 cttaccagct gcaagagaaa ccatttttgt ttttggccga tacaaaccgg cttcgtgtcc
    40921 caacgcacca aacgtttcgg taatttgtag cgcaaccagt tacgcgttga agcaaggatt
    40981 cgaacgtctt tgcggcttaa tcctacttct tcaaacaatt cacggtacat cgcctgctct
    41041 gcggattctc cggggttgat tccgccttgc ggaaattgcc aggagtgctg accaaatcgc
    41101 cgggcccaca ttacctgccc ctggcgatta caaatcacga tacctacgtt tgggcggtag
    41161 ccatcgtcat caatcaccgg actacctcaa aataaagctt tatatacgaa tgattgtttc
    41221 atactccagg aagacggtaa accactctct gcagggcatt acacactaat aacaattgaa
    41281 taactcacag ttatgtgcag agttataaac agaggaaggg gtggatagcc gtttttattc
    41341 accttttctg tggatagagt tgtgaagaac tacggaatta ctacgggaaa acccggagca
    41401 ttctgaataa gcccgatata gaaattttaa aatattcatt attagtcaat gcattacatg
    41461 taatttgagt aatgaaaaag agtaattcgt gacccaggtc acacctctca tttacgggtt
    41521 ggcgaaagat caaccaatgc cgtatttatc cacagaatgt gccactaagt taagcactga
    41581 accactaaaa actggagttt cgtcgcacgt caaggctgta aatgaaaaca gtagtggagg
    41641 tttttcacag ttatcccagc tttctgtgga taacatggtg taagatcctg tttattttca
    41701 gtgaccagat ttggaaaacc tattgcagtg ttgcgcaact cgattaccgg cagcctaaaa
    41761 agcaatataa atcagagaat tgaacaacac atgtggaaaa agttacactg cgaatattcg
    41821 gcacataatt actgtttgtt ttttaaccaa ggtatcatga catgtcccaa cctcgcccac
    41881 tgctctctcc tcccgaaact gaagaacagt tgttagcgca agcacagcaa ctttctggtt
    41941 atacattggg agaactggcg gcacttgccg ggctggttac gccagagaat ttaaaacgcg
    42001 ataagggctg gattggcgtg ttactggaga tctggctagg tgccagcgca gggagtaaac
    42061 ctgagcaaga ttttgctgct ctgggcgtgg aacttaaaac tatccctgtg gatagtcttg
    42121 gtcgtccgct ggaaacaaca ttcgtttgtg ttgccccgtt aacgggtaat agcggggtga
    42181 cctgggaaac cagccatgtg cgccacaagc tcaaacgcgt actgtggata ccggttgaag
    42241 gcgagcgcag catcccgctg gcgcagcgtc gcgtaggatc accgttgctg tggagcccga
    42301 atgaagagga agaccgacag ctacgcaaag actgggaaga attaatggat atgattgttc
    42361 tcggtcaggt tgagcggatc accgctcgtc acggggaata tttacagata agaccgaaag
    42421 cagcgaatgc gaaagcgctt accgaagcca ttggtgcccg gggcgaacgg attctgacgc
    42481 tgccacgcgg cttttatttg aagaagaatt tcaccagtgc actactggcc cgtcattttc
    42541 tgatccagta gccatcgctt tgacctgccg ctttccgggc atataattac cgcttcattt
    42601 ttttggcagg gctttttaga tgttatttgc atggataacc gatcctaacg cttggcttgc
    42661 gctcggtacg ctgacgctgc tggagatcgt tcttgggatc gacaatatta ttttcctttc
    42721 tctggtggtg gcaaagcttc ccacagcaca acgtgctcat gcgcgccgtc tggggttggc
    42781 gggagccatg gttatgcgtc tggcgctgct ggcatcaatc tcttgggtta cgcgcctgac
    42841 aaatccgctt tttacaatat tcagtcagga aatttctgcc cgtgatttga ttctgcttct
    42901 gggtggcttg ttccttatct ggaaagccag caaggaaatc cacgaatcca ttgaaggtga
    42961 agaagaaggg ctgaaaacac gcgtttcatc attcctcggc gctatcgtgc agattatgct
    43021 gctggatatt atcttcagcc tcgactcggt gattaccgct gtgggtctgt cagatcacct
    43081 gtttattatg atggcagccg tggtgattgc cgtaggcgtg atgatgttcg ccgcgcgctc
    43141 gattggtgat tttgtcgaac gccatccttc ggtaaaaatg ctggcgctct ctttcctgat
    43201 tctggtgggc tttaccctga ttctggaaag tttcgacatc cacgtaccga aaggttacat
    43261 ctacttcgcg atgttcttct ctattgcggt tgaaagcctc aacttgattc gcaacaaaaa
    43321 gaatccgctc tgatactccg tacgctctcc tgcgggaggg cgtactcact ccttttaccc
    43381 tcacagttta agattttcct gctttcaaga tatataacgt cggtttataa acagactatt
    43441 atcataggta ggccaaatga ggataaccgg atgaaaaaat gggcagtaat aatttctgca
    43501 gtcggactgg cgtttgctgt ttccgggtgt tccagtgatt acgtcatggc gaccaaagat
    43561 ggccgtatga ttttgaccga tggaaaacct gaaattgatg atgataccgg gctggtgagt
    43621 tatcacgatc agcaaggtaa cgcgatgcaa attaaccgtg atgatgtttc gcaaattatt
    43681 gaacgttaac aaataaggtc agcatccggc tggccttaag atttttctct ttcccttttc
    43741 ccttccctct gccattttta tattccttat gtcgtgatta taaaaaggaa acggctatgc
    43801 aatatcaccg tataccccac agttcgctgg aagtcagcac gctggggctt ggcacgatga
    43861 cgtttggtga acagaacagc gaagccgacg cccacgcaca actcgactat gccgtcgctc
    43921 agggcattaa ccttatcgac gttgccgaaa tgtacccagt acctccgcgc cccgaaacgc
    43981 aagggttaac cgaaacctac gtcggcaact ggctggcgaa acatggcagc cgcgaaaagt
    44041 taattatcgc ctccaaagtg agcggaccgt cgcgcaataa tgacaagggc atccgcccgg
    44101 atcaggcgct ggatcggaag aatatccgcg aagcgctgca tgacagcctc aagcgcctac
    44161 agactgatta cctcgatctt tatcaggtgc actggccgca gcgcccgacc aactgcttcg
    44221 gcaaactcgg ttatagctgg acggattctg cgcctgcggt ttcgctgctg gatacgctgg
    44281 acgcactggc agagtaccaa cgcgcgggaa aaattcgtta tatcggcgtg tcgaacgaaa
    44341 ctgcatttgg cgtaatgcgc tacctgcatc tggcggacaa acacgatctg ccgcgtattg
    44401 tcaccattca gaacccttac agtctgttaa accgcagttt tgaagtaggt ctggcagaag
    44461 tcagccagta tgaaggggtc gaactgctgg cctattcgtg cctgggtttc ggcacgctga
    44521 ccgggaaata tctcaatggt gcaaaacccg ctggcgcacg taatacgctc tttagtcggt
    44581 tcacccgcta tagcggtgag caaacgcaaa aagccgtcgc ggcgtatgtt gatatcgcca
    44641 gacgtcatgg ccttgatcct gctcagatgg cgctcgcgtt tgtacgctgt caaccgtttg
    44701 ttgccagcac tctgctgggc gcaaccacga tggatcagct gaaaactaac atcgaaagtt
    44761 tgcatctgga gttaagcgaa gacgtattag ctgaaattga agcggtacat caggtttata
    44821 cttatcctgc accataaaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc
    44881 gctgccagat ccacagcgcc gttattgcca gcgcaaatag cgcaccgaag ccaatgccaa
    44941 tgggcacgac cgggatgcct accattaccg ccagcgagta aatgcccagc atcaacaaca
    45001 tggcgctgtt ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt
    45061 taccccgctc ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc agcacgccaa
    45121 tcagcatcaa caaggcatag gctggcagca gctcgtgttg cagggagaaa atcggtacca
    45181 ccacgccaat caaaatcccg gctggcatac agcgtgacac ggtttccagc gtaaccagct
    45241 tcgctgccgc acctgcgcca accacgatac caatcactac catcgcgttg agataggtcg
    45301 gcgtagcgtt atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca
    45361 gcgtgacacc cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat
    45421 tgcaccatag cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg
    45481 actgccccgg acgcgccgcc agtttgggaa tgtagatatt ggcaacgacc gcaccaccgt
    45541 aggccagtgc gcatgcggcc agagcgacca ggacatgcca gtcagccagc acaccaccgg
    45601 ctacggaacc gagtaaaatc gccgctatgg cagaagcttc cattaaaccg ttagctttta
    45661 ctaacttact acccgtggtt aattcgccga gaataccgta tttcgccggt gaataggctg
    45721 cagcaccaac acccaccagc gtatagccga gaaacggatt gataccaaag cagatactgg
    45781 ctgcgcccag cagcttcagg ccgttggcaa acatcatcac ccggcctttg gcgaagctat
    45841 ccgccacctg cccgacaaac ggcgccaaaa gaatgtaagc acctacaaac accatttgca
    45901 ggatgggctg gctccactcc ggatagaact gcgctttcag taacgtcaga gtggcaaaca
    45961 gtagcgcgtt atcgccaaac gcagagagaa actgcgccac gataaccgct ttcatcccct
    46021 tcgaccacaa cgaagtgtta atgtgcactg actcactcat cgtgttgttc cgcttcgtct
    46081 acccagcttt tcaacgtgac aaagtcaggt ttgccgctgc caagtaatgg catctgtttc
    46141 agatagcgaa tatcgcgcgg tacagcaagc tccggcacgc cgtgctcgcg ggcatactgt
    46201 tgcaacttat cgcgcgtcag ttcgttatct gtggtgaaaa gcaccagtgc ctcgcctttg
    46261 ctggcatcgc tcttaatcgc agtggcatgg actttatctg gcgaaacacc aagtgccagt
    46321 tgttccacca tttccagcga caccatttcg cctgcaattt tggcaaagcg ttttgcgcgg
    46381 ccctgaatct gcacaaagcc ctgctcgtca aaacgcacaa tatcgccagt gtcataccag
    46441 ccgcgctcca tttcgccgcg aacattctcg gcggtgggca cttccagtac acctggcttc
    46501 tccacccgca gatagccgtt cattatgttc ggccctttca gttgcaggcg tccgccctct
    46561 tcgataccag ggaccgacaa caggcgtgca tccattcctg gtagaatacg ccctaccgta
    46621 ccgggtttcg ccgccatcgg tacgttgata gaaacgacag gcgcgcattc ggtcacaccg
    46681 tagccttcaa ggatgcgcag gccaaattta tcctgccaaa gctgtttggt actttcttgt
    46741 agtttttctg cgcctgccac cacatagcgt agacgataga agtcatacgg gttggcgaaa
    46801 cgcgcgtagt gaccgaggaa agtcgaggta ccgaacaaca cggtgcaact gcggtcatac
    46861 accaagtccg gcacaatgcg gtaatgcagc gggcttggat aaaggaacac ttctgcacct
    46921 gtaagcagtg gcgtaaacag gcctaccgtc agcccaaagg agtgaaacag cggtaatgcc
    46981 gacataaagc gatcgttggt ggtgaagtcg gcaatcgttt taatctgctc gacgttcgcc
    47041 agaatgcttt tatggctatg gacgacgcct tttggatggc cttcagaacc ggaggtaaag
    47101 aggatcagcg cctcttcttc cggctgctgt tttacctgcg ccaggcgcgg catcagcaaa
    47161 tgagcgaaga tccatacttt gtcggcagtg gtgacatctg cttttaaatc ttccagatag
    47221 acccagcgca cctgagtaag ttgctccggc agatgccaga gtttgccttt atcgagaaac
    47281 tggcgggaag tgaagatggt tttgatttca gccgccgtaa tagcactggt cagccctttt
    47341 accccggcag tgtagttcat cattgcggga atgcggcgac gcgcgatggc cccaaaaatc
    47401 actgccgcac tgatgcctgc attgggcagc attaagccga tgcgttcgcc ttcaacactg
    47461 tatttttcaa ggatgcgtcc aacaaacagc gttttcgtaa gcaatttgcg ataggagtct
    47521 ggggtaaagt tgacgtcttc gacacatttc ttcccggctc cgaagcggta cattgcactc
    47581 agtaaagatt cgtacagagt ttcacgcggg cgcaccgcca tacgcgcttc catcattatt
    47641 tgatgcagca tttcgccagc gattttgcga cggtcacggg cacgcggcgc atccggcatc
    47701 gccacctgcg ttggaggcaa aatatgcaga gtaatttgcg ggaacaagcg acgtttaacc
    47761 agacctttca ggcggctgaa gtgcgtaagt tccgcccctt caatacgcac aggaataacc
    47821 gttgcaccag acttcgccgc gacaaaaccc gcgccatcgt agattttcat cagcgagcct
    47881 gtagtggtga tgcgtccttc agggaaaatc accactggtc ggccctgttc caccagacgt
    47941 accagatgtt taatagccat aggttgcgtc gggtcgagag gaacaaagtc gataaatgat
    48001 ttcagccaac gcatatacca ctgttggctt attgaggtgt aaacggcaaa cactggacgc
    48061 acaggtaaaa acagtccaag caaaatgcca tcaataaaag agacgtgatt aggcgtaatt
    48121 agtacgcgct cgcccttcag tgcctgggtg tcacccgtaa cgcgaacgcg atacaaaaca
    48181 cggcacaaat ttcgaaaaaa gctaaaaagc attccaactc cctttgctct gattcagtaa
    48241 aagcgaatgg agggagatta cacgagataa agaacgcgag cgacagtaaa ttaggtgcga
    48301 aaaaaaacct gcgcatccgc gcaggttggt gcaagagaca gggtacgaag agcgtaccga
    48361 ataatctcac caatcaatac ctctgggatc ttgattgtgg tctgcacgac gactcttcgc
    48421 cagcgagaaa acgcaaagga atgaagggaa atgcaacgag gtgtgtaaat tgtcggttac
    48481 tgttacagat tgatgaccgg caaaaaaaaa cctgcgcatc tgcgcaggct ggtgtaattc
    48541 atgtgctcaa cacgaagttg acttcaccta tcaatacctc tgggatcacc actttagcaa
    48601 cctgaagcca aacgccacca gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag
    48661 gtttgcaatc tctttttttg tcttacttat ttgcgtttgg ctcacattcc cacgatgaaa
    48721 acacgccacc ccttgaacca acgggcgttt tccgtaacac tgaaacaatg taagcgttta
    48781 cccactaagg tattttcatg gcgaccataa aggatgtagc ccgactggca ggcgtttcag
    48841 tcgccaccgt ttcccgcgtc attaataatt cacccaaagc cagcgaagct tcccggctgg
    48901 ctgtgcatag tgcaatggag tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg
    48961 cgcagcagac cactgaaacg gtcggtctga tcgttggtga tgtttccgat ccgtttttcg
    49021 gtgcaatggt gaaagcggtc gaacaggtgg cttatcacac cggtaatttc ttattgattg
    49081 gcaacggtta ccacaacgaa caaaaagagc gtcaggctat tgagcaactg atccgccatc
    49141 gctgtgctgc gttggtcgtc catgccaaaa tgatcccgga tgccgattta gcctcattaa
    49201 tgaaacaaat gcccggtatg gtgctgatca accgtatcct gcctggcttt gaaaaccgtt
    49261 gtattgctct tgacgatcgt tacggtgcct ggctggcaac gcgtcattta attcagcaag
    49321 gtcatacccg cattggttat ctgtgctcta accactctat ttctgacgcc gaagatcgtc
    49381 tgcaagggta ttacgatgcc cttgctgaaa gtggtattcc ggccaatgat cggctggtga
    49441 catttggcga accagacgaa agcggcggcg aacaggcaat gaccgagctt ttgggacgag
    49501 gaagaaattt cactgcggta gcctgttata acgattcaat ggcggcgggc gcgatgggcg
    49561 ttctcaatga taatggtatt gatgtaccgg gtgagatttc gttaattggc tttgatgatg
    49621 tgctggtgtc acgctatgtg cgtccgcgcc tgaccaccgt gcgttaccca atcgtaacga
    49681 tggcgaccca ggctgccgaa ctggctttgg cactggcgga taatcgccct ctcccggaaa
    49741 ttactaatgt atttagtccg acgctggtac gtcgtcattc agtgtcaact ccgtcgctgg
    49801 aggcaagtca tcatgcaacc agcgactaac cgcagttaaa gcaattccag cgccagtaat
    49861 tcttcgatgg tctggcgacg gcgaatcaac cgcgcctgac cattatcaaa cagaacttct
    49921 ggtaacagcg gacggctatt gtagttggat gacattgatg cgccatatgc ccctgtatca
    49981 tgcagtacca gataatcacc tgccttcact tccggcaagg cgcgggtttc aacatttccc
    50041 ccttcctgct gggtaaagac atcgcccgat tcacataacg gcccggcgac gacggtttcc
    50101 accgttggcg cgtgttccag agaacgacca tcagctgcca gggcactgat atggtggtaa
    50161 ctaccgtaca ttgccgggcg catcagatcg ttgaacccgg catcaaccag cacaaagtgg
    50221 cggctaccca tttgtttgac gctccgcacc tgagtaatta atacgccaga ctgcgctacc
    50281 aggaagcgac ccggttcaat ttccagtttc acagggtggc ccaaatggcg agcgatttgc
    50341 tcacgcgcgg cattccacag accataataa tgttcggtat caaccgcctc ttcaccctgt
    50401 tgataaggaa tagaaagccc accgcccgca gaaatagcct gtaaatcctg accgaattcg
    50461 ataacctgac gcaccatagc accacacacc tgctccagat gggcataatc aacgccagaa
    50521 ccaatgtgca tgtgaatgcc gactagctgc agatgatgac gttgtatcac gtccagtgcg
    50581 gcgggcagat cggtgtacca gataccgtgc ttgctgtttt cgccaccggt attggttttt
    50641 tggctatgtc cgtgaccaaa ccccggatta acgcgcagcc atacccgatg ccctggcgaa
    50701 acctggccca gttggtcgag cgtatcaaca gaacccgcat tcaccggaat ttgcaattca
    50761 ctgacgcgtt caagcgtcgc ctgatcgata acatctgccg taaaaacaat atcatcgggg
    50821 tgcgtttgcg gattgtaacc cgccgccaac gcacgctcta tttcgcctaa cgagacggaa
    50881 tccactttca cgccctgctc acgcattaag cgcaaaatat gaatattgga acaggctttc
    50941 tgtgcaaagc gcaccacttc aaactgtttc agcgctgcaa tctgccgacg aataatttgc
    51001 gcatcgtaga cccacaccgg gcagccaaat tcagcgggca aacgcagcag attttcggcg
    51061 gtgagatcgg tatcggtgct gaacagtgaa tgtggcataa caaactccag atgagtgctt
    51121 ttttatgatt acgccacatc ataaaaagaa taaaaaatat cgatttatgt cgagtctatg
    51181 caaaaatgat atggattacc ggattgcgag agagcgctaa tggccgccgt taacttacgt
    51241 catattgaaa tttttcatgc ggtaatgacc gccggaagcc tgactgaggc agcacacctg
    51301 ctacacacct cacagccaac cgtcagccgc gaactggcgc gctttgagaa ggtgatcggg
    51361 ctgaaattgt ttgagcgcgt acgtggacga ttacatccta ccgtgcaagg actgcgtctg
    51421 tttgaagaag tgcaacgatc ctggtacgga ctggatcgca ttgtcagcgc cgcagaaagt
    51481 ctgcgcgagt ttcgccaggg agaactgtct attgcctgcc tgccggtctt ttcgcaatct
    51541 tttttaccgc agctcctgca accctttctg gcacgttatc ccgatgtcag cttaaatatc
    51601 gtgccccagg aatcaccgct acttgaagag tggctctcgg cccagcgtca tgatttagga
    51661 ctcactgaga cactccatac gcctacggga acagaacgta ccgaattact ctctttagat
    51721 gaagtgtgtg tgttacctcc gggtcatccg ctggcggtaa aaaaggtatt aacgccggat
    51781 gattttcagg gtgagaatta catcagcctt tcccgtactg acagctatcg ccagttgctg
    51841 gatcagctat ttactgaaca tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc
    51901 gcgtcagtct gcgcaatggt acgggcgggg gtaggtattt cggtggttaa cccgctcacc
    51961 gcactagatt atgcggcaag cggtttagtg gtgcggcggt tcagtattgc ggttccgttc
    52021 accgtcagcc tgatccgccc cctgcaccgc ccgtcatcag cgctggttca ggcgtttagt
    52081 gggcatttac aagcggggtt accgaaactg gtcacttctc ttgacgctat tttgtcgtca
    52141 gctacgacag cataaaagcg acagcatcct cggcatggat cgccgcggta tcaaacacag
    52201 gcaaaacact gcgctcttct ggcaccagta aaccaatttc tgtgcagcca aaaatgacgc
    52261 cctgtgcgcc ctgttctgca aggcgagcaa tcacttgcgc ataataagcg cgtgacgctt
    52321 cggtaaattg ccccagacac agttcttcaa aaataatctg attaattttc gcccgttcat
    52381 ccgcttcagg aataagacaa ttgatggaaa attgttccgt cagccgcccg cgataaaaat
    52441 cctgttccat ggtgtaacgc gtacccagca gcgccacacg agtcattccg gccccggtaa
    52501 ttgcacgtcc ggtggcatcc gcaatgtgta agaaaggcag agtgcaacgt gactcaatgg
    52561 catccgccac tttatgcatc gtattggtac ataacacaat accttctgcg cccgcccgct
    52621 gtaagccaag cgccgcctca gccagaatgt ccccggtttt atcccattcc ccgcgacgct
    52681 ggcactcttc tatttcatga aaatcgacgc tatgtagcag catttgcgca gagtgaagcc
    52741 caccaagccg ctgtttaatg ccttcattta tcagacgata gtaaggaatg gtggattccc
    52801 agctcattcc tcccagcaaa ccaattgttt tcattcctct ctcctgatgt gtgttaccgc
    52861 aacaaggctg aataaggaac gagacaaacg cctcaacggc caggtgccca atctctatta
    52921 acgaaaaaag ggccggatgt acagcacatc cggcccgtga aatcagacgc cgatatttct
    52981 caacttctcg cctgccatca gtttgcgttc gatatgttcc agcgtgacat ttttggtttc
    53041 cggaatgagc cagaaagtaa tacccacaaa cgcaatgttc agcgcagtgt agagccagaa
    53101 cgtaccggca gcgccaatgc tatcaagcag tgtcaggaag gccgcgccga taatcatatt
    53161 cgacacccag tttgtcgtcg tcgaacaggt aataccgaaa tcgcggcatt tcagcggctg
    53221 aatttcagag cacaggatcc acaccactgg cgcggcgctc atcgcataac cggcaataca
    53281 catcatcgtc atgccaacag agagccagga caagccactg gaagccgtac cgttatcaaa
    53341 ctgcatcagg caatagccca gcaccagagt gcctaacgcc atcacgctga aaccaatttt
    53401 cagagccggt ttacgacctg ctttatctac cgtaaagacc gcaatgaagg tcgcgaacat
    53461 aaaggtcagt cccacgacca gagtcgcaat catctgttgt tctgtggtcg taaagcccgc
    53521 cattttgaag atacgcggcg cgtagtacat gatgatgttc ataccggtaa actgctgcat
    53581 cgcctgcaac aacataccga gaaacacagc acgacggacg ttacggttga tcttaaacag
    53641 tgcccaaccg ccctgtttta acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc
    53701 tttttccgac gtatcgcgca gcatacgcaa cacttcttcc gcctcaatat gacgcccctt
    53761 ttccgccagc cagcgcgggc tatttggcag gaagaccacc agaataatca gcagaactgc
    53821 tggtaaagca agacccccca acattgcgcg ccagttaccg ctataactga acgctgtatc
    53881 ggataaaaac gccagcacga tgccgagtgt gaccatcaac tggtacatac tgatcatctt
    53941 accgcgaacg ttttcacttg ccatttcaga aagatacaga ggagcggtgt aagacgcgat
    54001 cccgacagca atgcccagca ccacacgagc ggcgattaac atctctacgc tggtcgcaaa
    54061 agcggaccct atagaaccga gtacaaacag gatggccccc gccatcaggc tgtatttacg
    54121 ccccaggcgg aacgacagcc aaccattaaa cagcgcacca attgctgcgc cgagcatcat
    54181 gctactaacc acccattcct gcaaacgact ggtcagcaca aagtgatcgg taatgaacgg
    54241 caacgctccg gcgattacgc cgatatcaag accaaataac aatcctgcga ccgcagcagc
    54301 taccgaaaca aacatattca tacgccgcgt atcacgcaaa gaacgtggcg ttaaagcaga
    54361 ttccgtattg atagtaacca ttttttcctg ccagcagaga gtaagacatt gtgaaaaaat
    54421 acgtgaacaa ttcacacagg tgtcaggtcg gaaacagcat aaatatggat taaattgctg
    54481 cgacatgccg ttatgtgatg gatattccaa ttttaaaatt aagttgaatt attgagatta
    54541 ttattaacct cctaatttta aagcagataa aattcataaa gttcattaat tgataattga
    54601 tatggattat ttcataacca tgatatggat tatgatgatc tagagatata aaaaaccctg
    54661 ccatgcggca gggtcataaa agtaagagga atgaattaac gcgccagcca accgccatcc
    54721 acagcaatgg cataaccatt tacataatct gaagcgctgg aggcaagaaa cactaccggc
    54781 cccatcaggt cactcggcag tccccaacgg ccagctggaa tgcgatcgag aatttccgcg
    54841 ctacgttgtt catctgcccg cagttgttgg gtattgttgg tcgccatgta acccggagct
    54901 atcgcattaa cattaatgtt gtgtttagcc cattcgtttg ccatcaatcg cgtcacaccc
    54961 atcacgccgc tttttgatgc ggtataagaa ggcacacgga tcccgccctg gaaggagagc
    55021 attgacgcga tattgataat cttgccgcca ttgccttgcg cgataaagtg tttcgccgct
    55081 gcctgagaca tgaagaatac gctcttgata ttcaggttca tgacatcgtc ccagtctttt
    55141 tcgctgaact cgagagcgtc ttcgcggcga atcaatccgg cgttattcac caggatatca
    55201 atatgaccaa attcagctac cgcgcgatcc agcagtccag gaatgccatc aatctttcgc
    55261 agatcggcgg tcaggcttaa aaaacgacgc ccctgcgccg tgacctgctc gatggtttca
    55321 gtcggttcaa cgatgttaat gccaacaatg tcacagcccg cttgcgccag ccccaacgcc
    55381 atcccctggc ccagcccagt atcacaacca gtgacgaccg caactttacc ttggagagaa
    55441 aatgcactta aaatcataac aacaccttat tcgtttatgc ccacaactag cgcaaatctt
    55501 taacgcccac atggtccata tcattaaaga cctggttttc accgaccatg ccccagataa
    55561 aggtataagc tttggttccg acgccggaat ggatcgacca gctcggggag atcaccgcct
    55621 gctcgttatg catcacaatg tgacgcgttt cttgcggctg ccccatcatg tggaaaacgc
    55681 aggcgtcatc atccatattg aaatagaaat aaacttccat ccggcgctcg tgggtgtgac
    55741 acggcatggt gttccacaag ttacctggag ccagctccgt cagccccata ctcaattgac
    55801 aggtttccag tacatccggg acaaaatatt tgttaatcgt gcgacggtta ctggtgaggt
    55861 tatcgcctaa cgtgactgga gatacttcgt ccggtgtgac ttttttggtg ggatacgtcg
    55921 tatgcgcggg tgcgcaattg taataaaact tcgccggagt ggcggtatca atactggcaa
    55981 agacaacttc ttttgcacct ttaccaacat acagggcgtc gcggtgaccg atttcatagc
    56041 attgaccatc gacagtaatc gtaccggcac cgccaatatt gataacacct aactcgcgac
    56101 gttcaaggaa atagcttacg ccgagttgtt taccaacttc cccgccaacg gaaaccgttt
    56161 tagttatcgg cataatgccg ccaacaataa ttcggtcaat gtggctgtaa accatggtgt
    56221 actcatcggc gacaaatacc ttttcaacca aaaattcatt gcgcagccct tgggtatcca
    56281 gcgtttttgc gtgcgcaccg tggatgctct gtcttacgtc cacatcaacc tccgataatc
    56341 agtgaagcgt gaaaatagaa aacgatagtg aaacactgtt tcgttttctc tataaaggga
    56401 tgatatgccc atgggctagg gttttcaatt aaaaataaaa caatgtttta tttttaattt
    56461 aaggattaaa aaaagtgcag tgtcgatcac gaatatgtca tgagaatgga aaaatgaggg
    56521 agcccagaaa atgtatgtcg acttgccagc catcattatt caacagatta atgacggcag
    56581 atatttatcg gtaaggagga aatgcagagc tactcgtcac gttcaatggt caatgccacg
    56641 ccctggcccc cgccgataca aagcgttgcc agccctttgc gggcattacg tttcaccatt
    56701 tcatgaacca gagaaaccag gattcggcaa ccggaagcgc ctatcgggtg accgagtgcg
    56761 atcgcgccac cattgacatt gacccgacgc tcatcccact caagcatctt gccaaccgaa
    56821 agcgcctgtg cagcaaacgc ttcattagcc tcgataagat cgacatcagc caactgccag
    56881 cctacacgct ccaggcaacg gcgggtcgca tacaccggcg caattcccat caatgccgga
    56941 tctacaccaa cgctggcaaa tgcgcggatg cgggccagca cgggtaaatt caacgctcgt
    57001 gctttggctt cgctcatcat cattaccgca gctgcgccat cgtttatgga tgatgcattc
    57061 cccgcggtca cagaaccgag actatcaaat gaaggattta aacgggctaa accttctgca
    57121 ctggcgtcag tgcgtggctg ttcatcggta tcaacaacca acgtctgacc gttactttgg
    57181 atcattaccg ggacgatctc atctttaaat cgtccggcgt caatcgccgc tcgcgctttt
    57241 tgttgcgagc taagtgcgta agcatcctgc aactgacggc tgatgccata ttcgcgagcc
    57301 agattttcgg cggtgacacc aatatgataa tcattgaagg catcccacaa cccatcatgc
    57361 accagactgt caaccaactg gctattgcca agctgtgcac cggtgcggct atcagtcaga
    57421 acatgtggtg cgcggctcat gttttcctgg ccaccggcga tgacaatatc agcctcgcca
    57481 cactgtatcg cctgagtagc cagatgcagt gctttaagac cggaaccgca aacgtcatta
    57541 atcgtgattg cagaaacgct gttaggaaga ccacctttaa tagccgattg ccttgccgga
    57601 ttctgccctg cccctgcagt caacacctga ccaagaatta cttcatccac cgcatatgca
    57661 ggaacgccgg tacgttctat taacgctttc acgaccagac taccaagttc cacggcggaa
    57721 tgacccgcta acgcaccacg aaagcagccg ataggtgtcc gtaacgcccc gacaatcaca
    57781 acgtctttca tcacaacctc gcaccaatta acagtgcaat agtaaatgat tgttaattac
    57841 agttatctta attgtttaaa aaaagtgatt tttatcacaa aggaaatatg cctgagcagt
    57901 agtcagagtc ataactggca cgtaaggttt gcaaccacta acccaccaat agatgggtag
    57961 atagggcgtt aatctcccat acttaacttg gtttatggta aattgccctc cattttgttt
    58021 aatttgtaga tgatacgttc agataatgtc taatatttgg tctaaagaag aaactctgtg
    58081 aagtttcgcg ctctatggca cagccgttga tgcaggcacg ctattccttc ctattcagtt
    58141 aggttcggca ggggctgtgg tcctgtttat tactgctctg gtcgcctggc ctttaacata
    58201 ttggccacat aaagccttat gccagttcat cctctcatca aaaacatcag caggtgaagg
    58261 gataacgggc gcggtaacac actactatgg caagaagatt ggtaatctga ttaccacgct
    58321 gtacttcatc gccttttttg tcgtcgtgtt gatatatgca gtggcaatta ccaactcact
    58381 tacggaacag ctggcaaagc atatggttat tgatcttcgc atccgtatgt tggtgagtct
    58441 gggtgttgta ttaattctga atctcatttt tctgatggga cgtcatgcca ctattcgggt
    58501 aatgggattt ttggtattcc cattgattgc ctatttctta tttctttcca tttaccttgt
    58561 cggtagttgg caacctgatc tattaacaac ccaggtagag ttcaatcaga atacccttca
    58621 ccagatatgg atatcgattc ccgtgatggt tttcgccttt agccatacgc ccattatttc
    58681 tacgtttgcc atagacagac gtgaaaaata tggcgaacac gctatggata aatgcaaaaa
    58741 aattatgaaa gtcgcttatc tcatcatctg cataagtgta ctgttctttg tctttagctg
    58801 cctgctttct attccatctt cgtatattga agcggctaaa gaagaagggg tcaccatttt
    58861 atcggcgctt tctatgctgc cgaacgcccc agcatggttg tcaatttccg ggattattgt
    58921 cgcagtagtt gcgatgtcga aatcattcct gggtacgtac tttggcgtta ttgaaggtgc
    58981 cacagaggtc gtcaaaacaa cactacagca ggttggtgta aagaaaagtc gtgcatttaa
    59041 ccacgcacta tcaattatgt tggtatcgct gattaccttc attgtttgtt gcattaaccc
    59101 gaacgcgatt tcgatgattt acgcgatcag cggcccgctc attgccatga tacttttcat
    59161 catgcctacg ctgtcaacgt atctcatccc ggcgcttaaa ccctggcgtt ccatcggaaa
    59221 tctgattacg ctgatcgtgg gtatcctgtg cgtatcggta atgttcttta gctaatatcc
    59281 gttgtggatg gttcaaaatt gctacaattt tgaaccattc catttttcat aatattcatt
    59341 tgttattatt ccgccctatc acttctctcg tcatatccta gaattatcag ttagttaaac
    59401 acacaaagag atctgaacat cttaaataag actatttaag atgcataact tagattcgca
    59461 agatatcttc tggtcattca aaaacaattt gcgatataga tattctcata ccatgagata
    59521 gttatctgga ccacgttcga ccatgcaaga ttaaatcagt gaaacataat catatttaat
    59581 aacgagaaat gcatttttaa atatcttaat cagaattata gcaaatacag attaatcgca
    59641 atttttccca gcaaaattac aaaatcaagg tgtattgaca tcatcaatca ccaatgaaat
    59701 tatgcaatta tatacggata gggaggttct taacatgggg ctttgtagtc gttataaaag
    59761 tcttacatgc aatagttgct caatgcattg ccaaataatg ccagaagagt caccgcgttt
    59821 gcagtactgc gcaaactcgt gtt
//
LOCUS       DAJVRP010000011        58067 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVRP010000011 DAJVRP010000000
VERSION     DAJVRP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.011"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..594)
                     /gene="ycjM"
                     /locus_tag="OQN12_000659"
     CDS             complement(<1..594)
                     /gene="ycjM"
                     /locus_tag="OQN12_000659"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCS1653115.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQI"
     gene            complement(806..1153)
                     /gene="pspE"
                     /locus_tag="OQN12_000660"
     CDS             complement(806..1153)
                     /gene="pspE"
                     /locus_tag="OQN12_000660"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1653116.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1196..1417)
                     /gene="pspD"
                     /locus_tag="OQN12_000661"
     CDS             complement(1196..1417)
                     /gene="pspD"
                     /locus_tag="OQN12_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1653117.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1426..1785)
                     /gene="pspC"
                     /locus_tag="OQN12_000662"
     CDS             complement(1426..1785)
                     /gene="pspC"
                     /locus_tag="OQN12_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1653118.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1785..2009)
                     /gene="pspB"
                     /locus_tag="OQN12_000663"
     CDS             complement(1785..2009)
                     /gene="pspB"
                     /locus_tag="OQN12_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1653119.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2063..2731)
                     /gene="pspA"
                     /locus_tag="OQN12_000664"
     CDS             complement(2063..2731)
                     /gene="pspA"
                     /locus_tag="OQN12_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1653120.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2898..3875
                     /gene="pspF"
                     /locus_tag="OQN12_000665"
     CDS             2898..3875
                     /gene="pspF"
                     /locus_tag="OQN12_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1653121.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3995..5260)
                     /gene="puuE"
                     /locus_tag="OQN12_000666"
     CDS             complement(3995..5260)
                     /gene="puuE"
                     /locus_tag="OQN12_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCS1653122.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5298..6578)
                     /gene="puuB"
                     /locus_tag="OQN12_000667"
     CDS             complement(5298..6578)
                     /gene="puuB"
                     /locus_tag="OQN12_000667"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCS1653123.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6580..8067)
                     /gene="puuC"
                     /locus_tag="OQN12_000668"
     CDS             complement(6580..8067)
                     /gene="puuC"
                     /locus_tag="OQN12_000668"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCS1653124.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8217..8774)
                     /gene="puuR"
                     /locus_tag="OQN12_000669"
     CDS             complement(8217..8774)
                     /gene="puuR"
                     /locus_tag="OQN12_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1653125.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8801..9565)
                     /gene="puuD"
                     /locus_tag="OQN12_000670"
     CDS             complement(8801..9565)
                     /gene="puuD"
                     /locus_tag="OQN12_000670"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCS1653126.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            9777..11195
                     /gene="puuA"
                     /locus_tag="OQN12_000671"
     CDS             9777..11195
                     /gene="puuA"
                     /locus_tag="OQN12_000671"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1653127.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11344..11508
                     /locus_tag="OQN12_000672"
     CDS             11344..11508
                     /locus_tag="OQN12_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653128.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11498..12883
                     /gene="puuP"
                     /locus_tag="OQN12_000673"
     CDS             11498..12883
                     /gene="puuP"
                     /locus_tag="OQN12_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1653129.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13017..13262
                     /locus_tag="OQN12_000674"
     CDS             13017..13262
                     /locus_tag="OQN12_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1653130.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13575..15218
                     /gene="sapA"
                     /locus_tag="OQN12_000675"
     CDS             13575..15218
                     /gene="sapA"
                     /locus_tag="OQN12_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1653131.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15215..16180
                     /gene="sapB"
                     /locus_tag="OQN12_000676"
     CDS             15215..16180
                     /gene="sapB"
                     /locus_tag="OQN12_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1653132.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16167..17057
                     /gene="sapC"
                     /locus_tag="OQN12_000677"
     CDS             16167..17057
                     /gene="sapC"
                     /locus_tag="OQN12_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1653133.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17057..18049
                     /gene="sapD"
                     /locus_tag="OQN12_000678"
     CDS             17057..18049
                     /gene="sapD"
                     /locus_tag="OQN12_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1653134.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18051..18857
                     /gene="sapF"
                     /locus_tag="OQN12_000679"
     CDS             18051..18857
                     /gene="sapF"
                     /locus_tag="OQN12_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1653135.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18925..19278
                     /locus_tag="OQN12_000680"
     CDS             18925..19278
                     /locus_tag="OQN12_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1653136.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19646..20434
                     /gene="fabI"
                     /locus_tag="OQN12_000681"
     CDS             19646..20434
                     /gene="fabI"
                     /locus_tag="OQN12_000681"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1653137.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20578..21705
                     /locus_tag="OQN12_000682"
     CDS             20578..21705
                     /locus_tag="OQN12_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCS1653138.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21773..23707
                     /gene="rnb"
                     /locus_tag="OQN12_000683"
     CDS             21773..23707
                     /gene="rnb"
                     /locus_tag="OQN12_000683"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1653139.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23785..23887)
                     /locus_tag="OQN12_000684"
                     /pseudo
     CDS             complement(23785..23887)
                     /locus_tag="OQN12_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23941..25915
                     /gene="pdeR"
                     /locus_tag="OQN12_000685"
                     /pseudo
     CDS             23941..25915
                     /gene="pdeR"
                     /locus_tag="OQN12_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26063..26236
                     /locus_tag="OQN12_000686"
     CDS             26063..26236
                     /locus_tag="OQN12_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653140.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26326..27075
                     /locus_tag="OQN12_000687"
     CDS             26326..27075
                     /locus_tag="OQN12_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1653141.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27344..27562
                     /gene="osmB"
                     /locus_tag="OQN12_000688"
     CDS             27344..27562
                     /gene="osmB"
                     /locus_tag="OQN12_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1653142.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(27688..28014)
                     /gene="yciH"
                     /locus_tag="OQN12_000689"
     CDS             complement(27688..28014)
                     /gene="yciH"
                     /locus_tag="OQN12_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1653143.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28014..28751)
                     /gene="pyrF"
                     /locus_tag="OQN12_000690"
     CDS             complement(28014..28751)
                     /gene="pyrF"
                     /locus_tag="OQN12_000690"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1653144.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(28944..30113)
                     /gene="lapB"
                     /locus_tag="OQN12_000691"
     CDS             complement(28944..30113)
                     /gene="lapB"
                     /locus_tag="OQN12_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1653145.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30120..30428)
                     /gene="lapA"
                     /locus_tag="OQN12_000692"
     CDS             complement(30120..30428)
                     /gene="lapA"
                     /locus_tag="OQN12_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1653146.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(30577..31341)
                     /gene="pgpB"
                     /locus_tag="OQN12_000693"
     CDS             complement(30577..31341)
                     /gene="pgpB"
                     /locus_tag="OQN12_000693"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1653147.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            31511..32101
                     /gene="ribA"
                     /locus_tag="OQN12_000694"
     CDS             31511..32101
                     /gene="ribA"
                     /locus_tag="OQN12_000694"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1653148.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32165..34840)
                     /gene="acnA"
                     /locus_tag="OQN12_000695"
     CDS             complement(32165..34840)
                     /gene="acnA"
                     /locus_tag="OQN12_000695"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCS1653149.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35004..35099)
                     /locus_tag="OQN12_000696"
     CDS             complement(35004..35099)
                     /locus_tag="OQN12_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653150.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35213..35380)
                     /locus_tag="OQN12_000697"
     CDS             complement(35213..35380)
                     /locus_tag="OQN12_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653151.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35383..35547)
                     /locus_tag="OQN12_000698"
     CDS             complement(35383..35547)
                     /locus_tag="OQN12_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1653152.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(35842..36816)
                     /gene="cysB"
                     /locus_tag="OQN12_000699"
     CDS             complement(35842..36816)
                     /gene="cysB"
                     /locus_tag="OQN12_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1653153.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37026..39623)
                     /gene="topA"
                     /locus_tag="OQN12_000700"
     CDS             complement(37026..39623)
                     /gene="topA"
                     /locus_tag="OQN12_000700"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1653154.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40003..40254
                     /locus_tag="OQN12_000701"
     CDS             40003..40254
                     /locus_tag="OQN12_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1653155.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40290..41339)
                     /gene="sohB"
                     /locus_tag="OQN12_000702"
     CDS             complement(40290..41339)
                     /gene="sohB"
                     /locus_tag="OQN12_000702"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1653156.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            41559..42317
                     /locus_tag="OQN12_000703"
     CDS             41559..42317
                     /locus_tag="OQN12_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1653157.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42314..42904
                     /gene="cobO"
                     /locus_tag="OQN12_000704"
     CDS             42314..42904
                     /gene="cobO"
                     /locus_tag="OQN12_000704"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCS1653158.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(42944..43816)
                     /gene="rluB"
                     /locus_tag="OQN12_000705"
     CDS             complement(42944..43816)
                     /gene="rluB"
                     /locus_tag="OQN12_000705"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1653159.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(43917..44537)
                     /locus_tag="OQN12_000706"
     CDS             complement(43917..44537)
                     /locus_tag="OQN12_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1653160.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(44534..45415)
                     /gene="yciV"
                     /locus_tag="OQN12_000707"
     CDS             complement(44534..45415)
                     /gene="yciV"
                     /locus_tag="OQN12_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1653161.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            45553..45597
                     /gene="trpL"
                     /locus_tag="OQN12_000708"
     CDS             45553..45597
                     /gene="trpL"
                     /locus_tag="OQN12_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS1653162.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    45574..45667
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            45689..47251
                     /gene="trpE"
                     /locus_tag="OQN12_000709"
     CDS             45689..47251
                     /gene="trpE"
                     /locus_tag="OQN12_000709"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1653163.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47251..48846
                     /gene="trpD"
                     /locus_tag="OQN12_000710"
     CDS             47251..48846
                     /gene="trpD"
                     /locus_tag="OQN12_000710"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1653164.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            48847..50208
                     /gene="trpCF"
                     /locus_tag="OQN12_000711"
     CDS             48847..50208
                     /gene="trpCF"
                     /locus_tag="OQN12_000711"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1653165.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50220..51413
                     /gene="trpB"
                     /locus_tag="OQN12_000712"
     CDS             50220..51413
                     /gene="trpB"
                     /locus_tag="OQN12_000712"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1653166.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51413..52219
                     /gene="trpA"
                     /locus_tag="OQN12_000713"
     CDS             51413..52219
                     /gene="trpA"
                     /locus_tag="OQN12_000713"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1653167.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            52600..52779
                     /locus_tag="OQN12_000714"
     CDS             52600..52779
                     /locus_tag="OQN12_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCS1653168.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            52865..53365
                     /locus_tag="OQN12_000715"
     CDS             52865..53365
                     /locus_tag="OQN12_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1653169.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53411..53917
                     /locus_tag="OQN12_000716"
     CDS             53411..53917
                     /locus_tag="OQN12_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1653170.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(53977..54615)
                     /gene="ompW"
                     /locus_tag="OQN12_000717"
     CDS             complement(53977..54615)
                     /gene="ompW"
                     /locus_tag="OQN12_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1653171.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            54855..54944
                     /locus_tag="OQN12_000718"
                     /pseudo
     CDS             54855..54944
                     /locus_tag="OQN12_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            54972..55715
                     /locus_tag="OQN12_000719"
     CDS             54972..55715
                     /locus_tag="OQN12_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1653172.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            55745..56284
                     /locus_tag="OQN12_000720"
     CDS             55745..56284
                     /locus_tag="OQN12_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1653173.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56389..56787
                     /gene="yciA"
                     /locus_tag="OQN12_000721"
     CDS             56389..56787
                     /gene="yciA"
                     /locus_tag="OQN12_000721"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1653174.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(56827..57555)
                     /gene="tonB"
                     /locus_tag="OQN12_000722"
     CDS             complement(56827..57555)
                     /gene="tonB"
                     /locus_tag="OQN12_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS1653175.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            57770..58066
                     /locus_tag="OQN12_000723"
     CDS             57770..58066
                     /locus_tag="OQN12_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS1653176.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        13937 a        15011 c        14526 g        14593 t
ORIGIN      
        1 aatttgatcg tcactaaagg tggtccataa atggcgcgta gaattatcat ccatttgaaa
       61 cggcgttaat agcggtaacg cacgcggaag ggtgacggca ctgagatcgg ttttcggatc
      121 aaccgcaata aagaaatctt caaaacccgg aagctgttgt aaatagtttt taaaccattc
      181 actttttgca gacatatggt tgcagacaaa atcaaacatt aaatgactgc attcaccgag
      241 ttgctgaata tcctgccact cccccgcttc actggcgacc tgatgataat caattaccga
      301 aaatccatca tcagatgacc atgggtaaaa aggtaataaa tgaacatgtg aaaaaatgct
      361 ttgcagccat tgatggtaga actgattaaa tatgggtagt ggttttaaat cattactgtg
      421 aaattgatcg gcataggtaa ttaacacgac atcactttca tcccagtgtt ttttacgtcg
      481 ctgtgtaatt aatcgtttcg cactctcaag acgcgttaca agtttctgta aatgagttgc
      541 ggtaaatgtt ccaccgtaga tttcgtccat gtaatccgta attttctgtt tcatgttgcc
      601 tttccgtggt agcggtccca tgaggcgaga gactactccg cagaaaataa actgtcaatt
      661 gagcacaagg gttttagtca cgcaggaaat cattttttac cagggaaaaa gcgtatttgt
      721 gagctgacgc aaataatcta tagggatgta aataaggtgt gacagaaaaa aaacggcgca
      781 taagcgccgc tcatggtgaa ttcttttaac ctttgacctt cggcattgcg atgtctttca
      841 ggccaccggc gttctcaacg tgggtatatc ccatctcgct aaggagctct tttgcctgcc
      901 cggactggcg tccggcattg caatacactt tcacggtgtc gtttttatcc ggaacggcgg
      961 tggcaatgcg ctctttcact tccttcaggg gaatattgat ggccccctga acgtgctctt
     1021 gctgatactg ctctggaaca cgaacatcga tccagtgttc agcggcgaaa acgggcagtg
     1081 aaaacaccag tgccagagca agtaagcctt tttttaaaca tgtaagcgtc cttctatgga
     1141 tgaaaattgc catccattat acgcctgaga tttaacaatt tttgcgaaac tcccctcacc
     1201 ttttataacg ctgtgccagt ttattagcag cccgacttaa cagcggttcc agcgccacag
     1261 ccagcaacat tttcagcggt cggcgagcaa ctgattttat cgcccagccc gccacacccg
     1321 ccgggccata gcgcagtgcg gtaagaagta ccagcttgcc tgctaatttg aaaccaagct
     1381 tcaccttttg cccggcctgt tgccagcgag tattcataac tttcctcaca gttgacggaa
     1441 acggctacgt aacgtgaaag tatcggaagt gacataacgt tccatctcgc gtaaacgcgt
     1501 ttcacttgcc gccagttcac ggtcgacttc atccagcaat tcgctgctgg aaggtagctg
     1561 ctcaccaaag gccatgttgt ccggcattgg atcaagcgca aatgacaaaa tgatgtaagc
     1621 aaccagggta aacagcgcca gaccgaagaa aatcgacagc accaccagga tacgcaccag
     1681 ttttaccggt acatcaaaat agttggcaat cccggcgcag acgccgcgga ccatgccctg
     1741 ctgtggaata cgccataact ttttattgag attaatgccc gccattagcg atccctccag
     1801 ttcggatgtt ctgcatcaag aatagattcc agcgcctgaa tacgttcgcg catccgtttt
     1861 gcttcatcaa ccagttgcgc taatcgctgc tgctcacttt gcgacaattc actgcgacca
     1921 gaacgattgc tgtaatgcag ccataaccag atcggtaaaa caaacagcac aaaaatggtt
     1981 aacggaatag ccagaaatag cgcgctcata agtactcctt agacgatgag cggcggcgcg
     2041 ttcggacgcc gcctggatgt tattattgat tgtcttgctt cattttggct tttaattgtg
     2101 ccagttgttc gctgattgca tcatcggctt tcagttcggc aaactgatcg tccagcgatt
     2161 tttgtttacc gaagctgtgg ctttctgctt ccgcttccat ctggtcaata cgacgttcga
     2221 aagattcgaa acgagccatt gcttcatcca gtttgccact gtccagctga cgacgcacat
     2281 cgcgcgacga gtttgccgcc tgatgacgta acatcaatgc ctgctggcga gcgcgtgttt
     2341 cgctcaattt gttttccagc tcgccaatct ctttcttcat gcgtgccagc gtatcgtcca
     2401 ccagcgtcac ttcatgttcc agggacttaa tcagatcggt cagtttctgt ttttcaatta
     2461 acgctgcacg tgccagatcc tctctctctt tcagcagcgc cagttcggct ttttcctgcc
     2521 attcaacttc acgcgccgac gcttgttcaa tacggcgagt cagctgtttc ttttctgcca
     2581 acgcacgcgc cgaagtagaa cgtacttcaa ccagtgtatc ttccatctcc tggatcatca
     2641 gacgcaccag tttctgtgga tcttccgctt tctctaacag agcgttgatg ttggcattca
     2701 cgatgtcggc aaagcgagaa aaaataccca taatgttgtc ctcttgattt ctgcgttcag
     2761 gattgtcctg ctgaactgtt aatacaattt gcgtgccaat tttttatctt tttgatttat
     2821 aaagatctga ttgaagaatc aacagcaaca tgccaggatg aattagcgaa ttacactaac
     2881 aagtggcgaa tttcatcatg gccgaataca aagataattt acttggtgag gcgaacagct
     2941 ttctcgaagt gctggaacag gtttcgcatc tcgcaccgct ggacaaaccg gtgctcatca
     3001 tcggcgaacg cggcaccggt aaagagttga ttgccagccg cctgcattat ctctcctccc
     3061 gttggcaagg gccgtttatt tcccttaact gcgcggcgtt aaatgaaaat ctgctggatt
     3121 ccgaactgtt tggtcacgaa gcgggggcgt ttaccggtgc gcaaaaacgt catcctggga
     3181 gatttgaacg tgccgacggc ggtacgctat ttcttgatga actcgctacg gcaccgatga
     3241 tggtgcagga aaaattattg cgtgtgattg agtacggtga actggagcgc gttggcggta
     3301 gccagccgtt gcaggtgaat gtgcggttgg tatgcgcgac gaatgccgat ctcccggcga
     3361 tggtcaatga aggtactttt cgcgctgacc tgctcgaccg gctggctttc gatgtagtgc
     3421 aactgccgcc cctgcgcgag cgcgaaagcg acattatgct gatggcagaa cactttgcca
     3481 ttcagatgtg tcgggaaatc aagctgcctc tgttcccggg gtttacggag cgcgccagag
     3541 aaacattgct gaattatcgt tggccgggaa atattcgtga attgaaaaac gtggtggaac
     3601 gttcagtgta tcgccacggc accagcgatt atccgcttga tgacatcatt attgatccct
     3661 ttaaacggcg tccacctgaa gaagccatcg ccgtttcaga aaatacctcg cttccgacgc
     3721 tgccgctgga tttacgcgag tttcagatgc agcaggaaaa agagttgctg caactcagtt
     3781 tgcaacaggg gaaatataac cagaagcgcg cggctgaatt actggggtta acttatcatc
     3841 agttccgcgc gttgttgaaa aagcaccaga tttagcgaag ttgagcaata caacttgccg
     3901 gatgcggcgt gaatgcttta ttcggcatac ataatcaata cgtcgtttgc aggcctgata
     3961 agcgcagcgc atcagacatt attgcgttgg gctattaatc gctcagcgca tcctgcaaaa
     4021 ttttcattgc cgcatcgaat tgcgcatccg ggatggtcag cggatacagg aagcgaatca
     4081 cgttgccgta tgcgccacag gtcagcagga gcagcccctg cgccagcgcg cgttgctgga
     4141 ttttctgtgc aatcgccgct gacggctcgc ccgtttgcgg atcgttaaac tctgccgcaa
     4201 tcatcgaccc caggccgcgt accgcagcaa tggccggaac gctttctttg gcatcaatca
     4261 acgtgttttt gagacgctgg cccagttgat tcgcgcgttc gcagagtgat tctttgtcga
     4321 taatattgag caccgcgtgc gcggcagcca ccgccagcgg gttcccggcg taggtgccac
     4381 ccaacccgcc cggcgcgggc gcgtccataa tattcgcgtt accgaccacg cccgaaagcg
     4441 gcatcccgcc cgcgaggctt ttcgccatcg tcattaagtc cggcttatcg gcgtaatgat
     4501 ccatggcaaa cagtttaccg gtacgcgcaa agccgctttg cacttcatcg gcaatcatca
     4561 caataccgtg ctcgtcgcac aggcggcgaa tggcggcaac cagctctttt ggcgcaacgt
     4621 tgaaaccgca ctcgccctgc accggttcga aaataatggc cgcgacctgc ttcgcttcga
     4681 tgtctgattt aaacaagcgt tcgatggcgt cgagggagtc ctgtgttgaa atgccgtgta
     4741 aatctgacgg ataaggtacg tgatacaccg aaccagggaa cgggccgaag ccgattttgt
     4801 acggcgcaac ttttccggtc agcgccatgg tcatatatgt acgaccgtgg aagccaccgc
     4861 taaacgcaat cacgccaggt cgtccggtat gggcgcgagc aattttcacc gcgttttcca
     4921 ccgcttccgc gccggtagtg aagaatgcgg ttttagcctg cccgctcact ggggcaaggg
     4981 tgttaatttt ctccgccagc gtgacgtagc tttcgtacgg cacaatctga tacgccgtgt
     5041 gggtaaactg ttgcaattgc tgctccaccg ccgcgaccag atcagggtgg cgatgcccgg
     5101 tattcagcac cgcaatgcct gcggcgaaat cgatgtattc gttgccctct acgtccttca
     5161 gcgtagcgtt ttctgccgac tgggcgaaga agttacacat cacgccaacc ccgcgtgggg
     5221 tagcagaaag acgacgctga tggaattcat tgttgctcat gtttcacacc ttttgactct
     5281 tcactgttaa tcggaaatta aacgcccaga cgatcgcgca ggctgtaata agccgcgccc
     5341 atcgcggtaa atggcacgcg cagcgtgcgc ccgccgggga acgggtaatg cggcagattg
     5401 gcgaaggcat cgaaacgctc ggcgtcaccg cgcagcagtt cagcaatcaa acgcccggca
     5461 agatgggtac aggtgacgcc gtggccgctg taaccctgca tgtaatagat gttggtatca
     5521 aggcggccga actgcggcat gtgcgacaag gtcagcagga agttgcccgt ccagcggtaa
     5581 tcaattttca cgcccttcaa ctgagggaag gtttttagta gtttcggtac cacaagacgc
     5641 tcaacgtcat ccgggttgcg tgcaccatac actacacccc cgccgtacag caggcggttg
     5701 tcggcggtaa gacggtagta atcaagcaga tagttacagt cttccacaca gtagtttttc
     5761 gggatcagcg aacgggctaa atcttccgac agccgttcgg tggtgatcac ctgcgtgccg
     5821 cacggcatgc tgcgtttcgc tagttccggc tctactttat cgcccagata cgcattcccg
     5881 gcgacaatca catacttcgc cgtcacctga ccattggcag tccgaaccac ggctggcgtg
     5941 gtgtgctgga tctgcgtcac ggcggaaagc tcatacacgc gcccgccgtt caggcggatg
     6001 gcatcggctt caccaatggc gaggtttagc gggtgaatat gcccaccgct gtgatccagc
     6061 agcgcaccag tgtagcgatc gctggcgacc tcgcggcgaa tagcgttgac gtccagcagc
     6121 tccagctgtt tattgccgta gcgttcccag ttctctttct gctcttcgag tgtggcgagt
     6181 tgtttatcat tcatcgccac aaacagaccg ccggggcggt agtcgcagtc aatctgataa
     6241 cgtttgatac gttcgcggat gatctcgcca ccctcgaaca tcatgctgcc gagcatacgg
     6301 gcggtgtcca tgccgtagct tttttcgatc acgtcgatgt cgcggctgta ggagttcaca
     6361 agctgcccgc cattgcgccc gcttgcgcca aagccgatgc gtgaggcttc gagaaccact
     6421 acgtcaaagc ccgcttccgc cagatgcagc gcggaggaga gcccggtata gccgccgcca
     6481 accacgcaaa cgtcgcaggt gattgactca ttcagtgtgt cgaatggtgc atatttattc
     6541 gcactggcgg cgtagtaact gctggtatgt tcggtcattt caggcctcca ggcttatcca
     6601 gatggttttc agttcagtga atttttcaag ggcatgcagg gatttgtcgc gaccgttgcc
     6661 gctctgctta tagccgccaa acggcacggt catatcgccg tcgttgtagt tattgacgaa
     6721 gacggaaccg gctttcaggc gtcggctcat gcggtgcgcg cgggagaggt cgcgcgtcca
     6781 taccgccgcg ccaaggccgt actggctgtc gttggcaagc tgcagcgcct gatcttctga
     6841 tgtgaaacgg gtgaccacca gcaccggacc gaaaatctct tcgcggctta aggacgcatt
     6901 cgggtccaca tccacgaaga tggtcgggcc gatggccgct gccagcccgg cgttgcgacc
     6961 atccagcagc agttgcccct tgccttcgcc ttcacgaata aaggtgtgta ccgagtcagc
     7021 gtgagcgctg tcgattaagg tgcccatggt ggttgcggga tcaagtggat ggcccggctg
     7081 ccagttttgc gcctgctgtt ttaacagggc taagaattca tcggcgatgc tctcttccag
     7141 taacaaacgc gttccggcga tgcacacctg gccctggttg tagaagatgc ctgcggcggt
     7201 ggcgcttgcc gctttttgca gatccggaca atcggcgaaa acgatgttgg cgcttttgcc
     7261 gcccgcttcc agccagacac gtttcatgtt gctatcaccc gcgtctttca gcagttgttt
     7321 acccgtacgc gtggagccgg taaaggcgat ggcatcgata tcgttatgac gcgaaagcgc
     7381 ctgccccgct tcatgaccaa aacccgtcac cacgttcaac acaccatccg gcaagcctgc
     7441 ttctttcgcc agccccgcga gacgaatcgc actgagcggt gatttttcag acggttttag
     7501 aatcacgctg tttcccgccg ccagcgccgg gccgagtttc cagcaagtca gcaacagcgg
     7561 gaagttccac ggcacgatgg cggcaatcac gccgaccggt tcacgcacga tcatcgccag
     7621 ctcatggcta ctggtggtcg ccacttcgcc atacactttg tcgatcgctt cggcgtacca
     7681 gcgaatggcg cgcgccgcgc cgggaatatc atcacgcaga ctgtgacgaa tcggtttgcc
     7741 ggtgtcgaga gtttccagca gtgccagctc ttcggcgtgg gcttccatta aatcggcgag
     7801 tttattcagt accgctttac gtttagccgg agaagagagt gaccagtcgc cgcgttcaaa
     7861 tacgccgcgt gctgcgctca ccgcacggtc gatatcgacg ctcttgccgc gggcaatttt
     7921 cgccagcggt gcctgggtga ccggatcaac ggtttcaaag gtttcatttt ccgccgcagc
     7981 agtatattca ccgttaataa ataagcggtt ttcaatggcg agacttaacg ctttatcctg
     8041 ccagtaagcc agatgatgaa aattcattat gactcctgtt tcacgtctat cagatatatg
     8101 cagtttgtga acgtcagggc gcagagatcc ccgggctgca tgcccgttta tttctggcga
     8161 cattctttat ggattgccgg gcgcattgcg cttacccggc ttacagaaca aaaagattaa
     8221 aacgtggtgg gcgtatgggc gctgataatt cggcaaatac ctgccgacgt attactgaaa
     8281 ctgtgcggga tgccggtatt aatggcataa ctttgccccg cgacgaggtg gtaatcctga
     8341 ccattaatcg tcagaacaat ttcaccttcc agtactgtgc ctatttcctc accctgatgc
     8401 ttaattcttt ccccagtggt tgtgcccggc tggtacgttt caaagatcat cgccagcgtg
     8461 cgattcgggt taccgttatg aaccagcttc attgacacac cctgactgcc catctcaatt
     8521 aagtcgtcct gattaatgac gacctgcggc tcatcaggtt tttccggctc ggaaaagaat
     8581 tccgagagtg acaaaccata caccttcagc agcttttgca gcgtactgat ggcagggctg
     8641 actttatctt gttctatcgt actgatagca ctgtgagtca gcccggagag ttcggcggca
     8701 cgacgttgtg aaagcccctg ctgctggcgg atttccgaca agcgttttcc tggcgccagt
     8761 ccctcatcac tcatatttgc atttccttaa actgtagtgg tcagagtcgc tgtttttcag
     8821 cgatatggtg ctgccaagcg gtgataaagc cctcgaacaa tatacgcgaa agcgcgtact
     8881 cgctactgtt ccattccggg tgccactgta cgcccagcgc aaaaggatga ttgatgacgc
     8941 taaccgcctc aactaagcca tccggcgagc gagcttcaac acgcaaccgt gggctaacga
     9001 ccttcgcccc ttgtccatgt agagagttta cccaaaagtt gctacattca ggtaacaacg
     9061 cagacagtaa tcccccctct tcaacctgaa cttcatgcga cggtgcatat tgctgttcca
     9121 ccggcagttc gggatcttcc cgatgttcca gcaattcagg ctgttcgcac agcttgcgat
     9181 gcaacgaacc accggttgcc accaccagtt cttgtaaacc ccggcagatg gcgaaaatgg
     9241 ggatgcgcct ttcgagtgcg gcattaatta tcgccatgct cagaagatca cgcccgggat
     9301 cggcgtcagg ctcatcgccg ttttcaccat ataggtgcgg ctgcacattg ctgggactac
     9361 taggaagata aatgccatcg agtttcggca aaagttgttc aagtaacgac ggttccgcca
     9421 gcgcatgtgg tagcgcaatt ggcaagccgc ctgcgtggat gatggcattc aggtactttt
     9481 cttgcagagt ctgggtcgca tgacccttaa gcctgttcct gcacattacg acaccgataa
     9541 ccggattgtt cattatattt tccatgctca atctcacaaa gtggactaaa ttatcgccat
     9601 tactgcgctt taaatgacct ttatgttcaa tattttttca atctagcagt ggattttcat
     9661 ttttgcaaac tcaatttaac atttgacaaa catttggttt gcatacagat tcgaatgatg
     9721 gtcattatat tttacgcttt gataacgagc ggaaaacaaa ccaaaggcga agaatcatgg
     9781 aaaccaatat cgttgaagta gagaactttg ttcagcagtc agaagagagg cggggtagca
     9841 cctttacgca ggaagtgaaa cgctacctgg agcgctaccc gaatacgcaa tatgttgatg
     9901 ttttacttac tgatttgaat ggttgcttcc gtggaaaacg tatccctgtt tcaagcctga
     9961 agaagctcga gaaagggtgt tatttcccgg cctcggtatt tgcaatggat attctgggca
    10021 acgtagtaga agaagcaggt ctgggtcagg aaatgggcga gccggatcgt acctgtgttc
    10081 ctgttctcgg ttccttaact ccttctgccg ccgatccaga gtttatcggt cagatgctcc
    10141 tgaccatggt cgatgaagat ggcgctccct ttgacgttga gccgcggaac gttcttaacc
    10201 gcctgtggca gcagctgcgc cagcgcggat tgttcccggt tgtagcggta gagctggagt
    10261 tctatttact ggatcgccag cgcgacgctg aaggggatct gcaaccgccc tgcgcgcctg
    10321 gcaccgatga ccgcaataca caaagccagg tttactccgt tgataacctc aaccacttcg
    10381 ccgacgtgct caatgatatt gatgaactgg cgcagttgca gctgattccg gcagatggcg
    10441 cggtcgctga ggcctcgccg ggtcagtttg aaatcaacct ttaccatact gataacgtgc
    10501 tggaagcctg cgatgatgcg ctggcactaa aacgcctagt gcgtctgatg gcagaaaagc
    10561 ataagatgca cgccactttt atggcgaagc cgtatgaaga gcacgcgggc agcgggatgc
    10621 atatccatat cagtatgcaa aataatcgtg gcgagaatgt gctttctgac acagaaggcg
    10681 aagattcgcc gctgctgaaa aagatgctcg ccgggatgat tgacctgatg ccgtcgtcga
    10741 tggcgttgct ggcaccaaac gtgaactcgt atcgccgctt ccagccggga atgtatgtgc
    10801 cgacgcaggc gtcgtggggc cataacaacc gcaccgtcgc cctgcgtatt ccgtgcggtg
    10861 accgtcataa tcaccgcgtg gaatatcacg tggcgggtgc cgatgccaac ccatatctgg
    10921 tgatggcagc gatttttgcc ggtattttgc atggccttga taacgagctg ccgttgcagg
    10981 aagaagtcga aggcaacggg ctggaacagg aaggcttacc cttcccgatt cgccagagcg
    11041 atgccctggg tgagtttatc gagaatgatc acctgcgccg ctatttaggc gaacggtttt
    11101 gccatgtgta tcacgcctgt aaaaatgatg agctgttgca gtttgagcgc ctcatcacag
    11161 aaacagaaat tgagtggatg ttgaaaaacg cgtaattccc cttgcggggt tgtttcatcg
    11221 gcctgagcgg agccaggcct gacatttgct ggtaatccag acatttcccg gatgtcgcgt
    11281 tatggagcgc aggcggcaac gggcttccgt taacgattaa gactgtgacg aggaaatgag
    11341 atgatgccga tgattaaatc cccgcatggc gagggaggct gcgtatgcgc ccctcctgct
    11401 actgactgga ctccgccccc tttgcttccc ttgctaaaca ggtttgattt caggtcgaca
    11461 cgaccgcaaa ccttattacg caggggaggc agcaattatg gctattaatt caccactgaa
    11521 tattgctgcg caacccggca aaacccgtct gcgaaaatca ctgaaattgt ggcaggtggt
    11581 aatgatgggt ctggcctatc tcacgccgat gaccgtattt gatacttttg gtattgtctc
    11641 cggcattagc gacggtcacg ttccggcgtc ctatttgctg gcgctggcgg gcgtgctgtt
    11701 taccgctatc agctacggca aactggttcg ccagtttccg gaggccggtt cggcctatac
    11761 ctacgcgcaa aagtcgatta acccgcacgt cggatttatg gtcggctggt catcactgct
    11821 ggattatctc tttttgccaa tgatcaacgt tttgttggcg aaaatctatc tctccgccct
    11881 cttcccggaa gtgccgccgt gggtgtgggt ggtaaccttc gtcgccattt taaccgccgc
    11941 gaatctgaag agcgtcaacc tggtcgctaa cttcaatacc ctgtttgtac tggtgcaaat
    12001 ctccatcatg gtggtgttta tcttcctggt ggttcaggga ctgcataaag gagaaggcgt
    12061 tggcaccgtc tggtcacttc agccgtttat cagcgagaac gcgcacctga tcccgattat
    12121 taccggtgcg acgattgtct gtttctcgtt cctcggtttc gatgcggtga ccacgctttc
    12181 ggaagagacg ccggacgccg cacgcgtgat cccgaaagcc atcttcctga cggcggtata
    12241 cggcggcgtt atctttatcg cggcgtcgtt ctttatgcag ctgttctttc ccgatatcag
    12301 ccgctttaaa gacccggacg ccgcactgcc tgaaattgcg ctgtacgtcg gcggcaagct
    12361 ctttcagtcg attttcctct gcaccacgtt tgtgaacaca ttagcgtctg gcctggcttc
    12421 acatgccagc gtgtcgcgtc tgctgtatgt gatggggcgt gacaatgtgt ttccggagcg
    12481 cgtgtttggc tatgtgcacc caaaatggcg gactccggca ctcaacgtca ttatggtcgg
    12541 gattgtcgcg ctgtcggcgc tgttctttga tttagtcacc gcgacagcac tgattaactt
    12601 cggtgctctg gtggcgttta ccttcgtgaa tctgtcggtg tttaatcatt tctggcggcg
    12661 taaaggaatg aataaaagct ggaaggatca cttccactat ttgctgatgc cgctggttgg
    12721 cgcgctgacg gtgggtgtgc tgtgggttaa cctagagtca acgtcactga cactcggtct
    12781 ggtatgggct tcgttaggcg gcgcatattt gtggtatctg atccgccgct atcgcaaagt
    12841 gccgctgtac gaaggtgaca gaacgccggt gagcgaaacg taatagccgg atgcgcggcg
    12901 cattatcctg cctgagggta acgcgccgcg ccattaacta taaaaagatg tatgacccgg
    12961 atcacgagtg ctatcatccg cccttatgct tagcgcatta accccagggg caaataatga
    13021 accacgatat cccgctaaaa tattttgata ttgccgatga gtacgcgacc gaatgcgcag
    13081 agcctgtcgc agaggctgaa cgcacaccgc tggcccacta cttccagtta ctgctcaccc
    13141 gtttgatgaa caatgaagag atcagcgaag aagcccaaca tgaaatggct gctgaagcgg
    13201 ggattaatcc cgtacgcatt gatgagatcg cagagttttt gaatcaatgg ggtaatgagt
    13261 aattatttaa ttgcagacga tcggtcagta tcgttgtccg gataaagcgt tcacgccgca
    13321 tccggctaac aatgacacgc actgcagcgt gctttaatgg gtttctcacg atcgtaccgc
    13381 tctgctcctc gctaagttgt tctgtgagtt atagttatag agaaatcacg ggtacgaatg
    13441 ttgaacagat ggcgacgcca cctgttcaac gaccaaccac acggccctca ttacgacccc
    13501 tgaacacaaa cggtaatggc aaatgtcatt aaatattagt aagatagcct ctttacataa
    13561 aatcccttaa tattatgcgc caggtattat cgtctctttt ggtgattgct ggacttgtga
    13621 gtggtcaggc aatcgccgcg cctgaatccc ccccgcatgc tgatatccgc gacagcggtt
    13681 ttgtctattg cgtcagcggg caagtcaaca cctttaaccc atccaaagcg agcagtgggt
    13741 taattgtcga tacccttgcc gcccagtttt atgatcgact gctggatgtc gatccctata
    13801 cctatcgcct gatgcctgaa cttgccgaaa gctgggaagt actcgacaac ggcgcgacct
    13861 atcgcttcca cctgcgtcgc gatgttccgt ttcaaaaaac cgactggttt actcccactc
    13921 gtaaaatgaa tgccgacgat gtggtgttta ccttccagcg aatttttgac cgcaacaacc
    13981 cgtggcataa cgtcaacggc agcaacttcc cctacttcga cagcctgcaa tttgccgata
    14041 acgtgaaaag cgtccgcaaa ctggataatc ataccgttga gttccgactc gcccagccgg
    14101 atgcttcttt tttgtggcac ctcgcaaccc attatgcttc ggtcatgtcg gcagaatatg
    14161 cccggaagtt agagaaagaa gatcgccagg agcaactcga ccgtcaaccg gtcggcaccg
    14221 gtccgtatca gttgtcggaa taccgcgccg ggcaatttat tcgcctacaa cgtcatgatg
    14281 acttctggcg cggtaaaccg ttaatgccgc aggtagtggt ggatttaggc tccggcggca
    14341 ccggacgtct gtcgaaactc ctgaccgggg aatgcgacgt tctggcctgg cctgctgcca
    14401 gccagctatc cattttgcgt gacgacccgc gcttgcgttt aacgctgcgt cctgggatga
    14461 acgtcgccta tctggcattt aacaccgcca aaccgccgct aaataatccc gctgtccgcc
    14521 atgcactggc actggcgatt aataaccagc gcctgatgca atccatctat tatggtacgg
    14581 ctgaaacggc ggcctctatt ttaccgcgcg cctcgtgggc ctatgacaac gaggctaaaa
    14641 ttactgaata caatccggcg aaatcgcgcg aacagttgaa ggcgttgggg ctggaaaact
    14701 taacgctgaa actgtgggtg cccacacgtt cgcaggcgtg gaaccccagt ccactgaaaa
    14761 ctgccgaact gattcaggcg gatatggcgc aggttggcgt aaaagtggtg attgtgccgg
    14821 tagaaggtcg ctttcaggag gcgcggttga tggatatgag ccatgatctg acgttatccg
    14881 gttgggcgac ggacagtaac gacccggaca gtttcttccg tccgttactg agctgcgcgg
    14941 caattcattc acagaccaac ctcgcccact ggtgcgatcc gaaattcgac agcgtgttgc
    15001 gtaaggcgct ctcctcgcag cagctggcag cgcgtattga agcctatgac gaagcgcaga
    15061 gtattctggc gcaggaatta cctattctgc cgctggcgtc gtcattgcgt ttgcaggcct
    15121 atcggtacga tatcaaaggt ctggtactta gcccgtttgg taacgcctcc tttgctgggg
    15181 tgtatcgcga gaaacaggat gaggtgaaaa aaccatgatt atcttcacct tacgccgcat
    15241 tttgttattg attgtcacct tgttcctgct gaccttcgtt ggcttcagct tgagctattt
    15301 cactccacac gcgccgttac aaggcgcgtc actgtggaat gcctgggtgt tctggtttaa
    15361 tggcctgatc cactgggatt ttggtgtttc cagcattaac ggtcaaccaa ttgccgaaca
    15421 gttaaaagag ctcttcccgg cgacgataga actgtgcatc ctcgccttcg gttttgcgct
    15481 gattgttggc atccccgtgg ggatgattgc cgggattacg cgccataagt ggcaggataa
    15541 cttgatcaac gccatcgcct tactgggctt ttcaatcccg gtgttctggc tggcgcttct
    15601 gttgacactg ttttgttcac tcacgctagg ttggttgccc gtttcagggc gtttcgatct
    15661 gctctacgaa gtgaaaccga ttaccggttt tgcgttgatt gatgcctggc tttcggactc
    15721 accatggcgg gatgaaatga tcatgagcgc aatccgccat atgatattgc ccgtgatcac
    15781 cctgtcggtt gcgccaacaa ctgaagtgat ccgcctgatg cgtatcagca ctattgaagt
    15841 gtatgaccag aactatgtaa aagcggcggc gacccgcggt ttgtcgcgct ttactatttt
    15901 gcgtcgccac gttctgcata acgcgttacc gccggttatt cctcgcctgg gcttacagtt
    15961 ttccaccatg ctgacgctgg cgatgatcac cgaaatggtt tttagctggc cgggtttagg
    16021 gcgctggtta attaacgcca tccgccagca ggattacgca gccatttccg ccggagtgat
    16081 ggtgtgtggc tcactggtca ttattgttaa cgtgatttct gatattttgg gtgccatggc
    16141 taaccctctg aaacataagg aatggtatgc cttacgatag cgtatacagc gaaaagcgcc
    16201 cgccgggcac gctgcgtacc gcctggcgca aattttatag tgacgcctct gcgatggtcg
    16261 gcctgtacgg ctgcgcggga ctggctgtac tgtgtatttt tggcggctgg tttgcgcctt
    16321 acggcatcga ccagcaattt ctcggttatc aattattgcc gccatcatgg tcgcgctatg
    16381 gcgaagtttc tttcttcctg gggactgacg acctcggacg tgatgtttta agccgtttac
    16441 tgagcggagc tgcgccaacc gtgggtggcg catttgtggt gacgcttgcc gcgacgatct
    16501 gtggcctggt actgggcacc ttcgccgggg cgacacacgg cctccgctca gcggtgctta
    16561 accatattct ggatacctta ctggcgatcc cttcgctgct gttggcaatt atcgttgtag
    16621 cgtttgccgg accgagtttg tcccacgcta tgtttgctgt ctggctggca ttgctgccgc
    16681 gtatggtacg ttcgatttac agcatggtgc atgacgaact ggaaaaagag tacgttatcg
    16741 ccgcccgtct ggatggcgca tcaacgctga atattctctg gtttgctgtg atgccaaaca
    16801 tcaccgccgg gctggtcacc gagatcaccc gcgcactgtc gatggcaatt ctcgatatcg
    16861 ccgcgctggg ctttctcgat ctcggcgcac aactcccctc gcctgaatgg ggagcgatgc
    16921 tcggtgatgc gctggaactg atttatgtcg ccccgtggac tgtcatgctg ccaggtgcgg
    16981 caattatgat tagcgtgttg ttggttaacc tccttggcga tggcgttcgt cgtgcgatta
    17041 ttgcgggggt ggaataatgc cattactcga tattcgtaac ctgaccattg aatttaaaac
    17101 cggtgatgag tgggttaaag ccgtcgaccg ggtaagtatg accttaaccg aaggtgaaat
    17161 ccgcggtctt gttggtgaat ccggttccgg caagagtttg attgcgaaag cgatttgtgg
    17221 ggtgaataaa gataactggc gtgttactgc tgaccgtatg cgttttgatg atatcgattt
    17281 gctgcgtctc tccgcacgcg aacggcgcaa actggttggc cataacgtgt cgatgatttt
    17341 ccaggaaccg cagtcgtgtc ttgacccttc agaacgtgtg ggtcgccagt tgatgcaaaa
    17401 catcccagcc tggacctata aaggccgttg gtggcagcgt tttggctggc gcaaacgccg
    17461 tgccattgaa ctgctgcacc gcgtggggat taaagatcac aaagatgcga tgcgcagttt
    17521 tccctatgag ttgaccgaag gtgaatgtca gaaagtgatg atagccattg cactggcgaa
    17581 tcaaccgcgt ctgctgattg ctgacgaacc gacaaactca atggagccaa caacccaggc
    17641 gcaaatcttt cgcctgctga cgcgtctcaa ccaaaacagc aataccacta ttttgcttat
    17701 cagccatgac ttacaaatgc ttagccaatg ggcggataaa attaacgtgc tttactgcgg
    17761 tcaaacagtg gaaaccgcgc cgagtaagga gttggtgacg atgccacatc atccttatac
    17821 ccaggcgctg atccgcgcga taccagactt cggcagcgcg atgccgcata aaagtcgcct
    17881 caatactcta cccggcgcta tcccattgct ggaacagtta ccgattggtt gtcgtctggg
    17941 gccacgttgc ccgtatgcac aacgagaatg cattgtgacg ccacgtttga cgggggcgaa
    18001 aaatcatctc tatgcctgtc atttcccgct gaacatggag aaaaagtgag atgatcgaaa
    18061 cgctgcttga agtgcgtaat ttaagtaaaa cgttccgcta ccggaccggt tggtttcgtc
    18121 gtcagaccgt agaagcggta aaacccttga gctttacgct acgtgaaggc cagacactgg
    18181 cgattattgg cgagaatggt tcgggtaaat ccacactggc aaaaatgctg gcgggaatga
    18241 tagaacctac cagcggcgag ttattgattg acgatcatcc gctgcatttt ggcgattatt
    18301 ccttccgtag tcagcgtatt cgcatgattt ttcaggatcc ttcgacctcg ttgaatcccc
    18361 gtcaacgtat ctcacaaatt ctggattttc cactgcgcct gaacaccgac ctggaaccgg
    18421 aacagcgtcg caaacagatt attgaaacga tgcgtatggt tgggctgcta ccggatcacg
    18481 tcagttacta tccgcatatg ttggcacccg gacaaaaaca gcgtctgggt ctggcgcgcg
    18541 cgctgatatt gcgcccaaaa gtcattattg ccgatgaagc cctcgcttca ctggatatgt
    18601 caatgcgttc gcagttgatt aatctgatgc tggagttaca ggaaaaacag ggcatttcgt
    18661 atatttatgt tacccagcat atcggaatga tgaagcacat tagcgaccag gtgctggtga
    18721 tgcatcaggg cgaggttgtc gagcgaggca gcaccgcgga tgtgctggca tcgccgctgc
    18781 atgaactcac caaacggctg attgccgggc actttggcga agcattgaca gcggatgcgt
    18841 ggcgtaaaga tcgctaattt tctgaatcct ccttccagat tattcacctc attctgacaa
    18901 gagattaaat cttgtcagaa tgaggtgatg gataaaataa aatcaaatgc gcgtgattta
    18961 agacgcaatc tcactttgca ggaacgaaag ctctggcgat atcttcgcag ccgacgtttt
    19021 ggtgatttca aatttcgccg tcaacatccg gtggggagct acattctcga ttttgcttgc
    19081 tgctcggcgc gcgtagtcgt tgagctggat ggtgggcagc atgatttggc agttgcctat
    19141 gataccaggc gcactagctg gcttgagtcg cagggctgga ccgtgctgcg tttctggaat
    19201 aacgagattg attgtaatga ggaggcggtg ctggagatta ttctgcagga actgaaccgc
    19261 cggtcaccct caccctgaaa gggcgagggg gccgaccgag ccgaatagct gttgtggtga
    19321 aaacatggag acagtgctgg agaatattcg gcaaggtctg aaccatccca gccatcgcca
    19381 taaaagggtt aggggctgta tgagcctgtt tgttgctggg gtaacaatat ttgcacaata
    19441 tggtcccctc gcccctctgg ggagagggtt agggtaaggg gaaaagcgcc ccccctgccg
    19501 cagcctgctc cggtcggacc tggcaactat agctactcac agccaggttg attataataa
    19561 ccgtttatct gttcgtactg tttactaaaa cgacgaatcg cctgattttc aggcacaaca
    19621 agcatcaaca ataaggatta aagctatggg ttttctttcc ggtaagcgca ttctggtaac
    19681 cggtgttgcc agcaaactgt ccatcgccta cggtatcgct caggcgatgc accgcgaagg
    19741 agctgaactg gcattcacct accagaacga caaactgaaa ggccgcgtag aagaatttgc
    19801 cgctcaattg ggttctgaca tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga
    19861 caccatgttc gctgaactgg ggaaagtttg gccgaaattt gacggtttcg tacactctat
    19921 tggttttgca cctggcgatc agctggatgg tgactatgtt aacgccgtta cccgtgaagg
    19981 cttcaaaatt gcccacgaca tcagctccta cagcttcgtt gcaatggcaa aagcttgccg
    20041 ctccatgctg aatccgggtt ctgccctgct gaccctttcc taccttggcg ctgagcgcgc
    20101 tatcccgaac tacaacgtta tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta
    20161 tatggcgaac gcgatgggtc cggaaggtgt gcgtgttaac gccatctctg ctggtccgat
    20221 ccgtactctg gcggcctccg gtatcaaaga cttccgcaaa atgctggctc attgcgaagc
    20281 cgttaccccg attcgccgta cagttactat tgaagatgtg ggtaactctg cggcattcct
    20341 gtgctccgat ctctctgccg gtatctccgg tgaagtagtc cacgttgacg gcggtttcag
    20401 catcgctgca atgaacgaac tcgaactgaa ataatcgttc tgttggtaaa gatgggcggc
    20461 gttctgccgc ccgttatctc tgttatacct ttctgatatt tgttatcgcc gatccgtctt
    20521 tctccccttc ccgccttgcg tcaggataac gatttccttt acgaccaagg agcgcccatg
    20581 gaacaacgcc acatcaccgg caaaagccac tggtatcatg aaacgcaatc cagtactacg
    20641 gagtatgacg ttctgcctct ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc
    20701 gacgtgatcc ttgatgaaga gacgctggcc cccttccttt catggctggt ccctgcgcgc
    20761 gttcttgcag tggagttgtt ccctgaccag cttaccgtga cccgttcaca aaccttcacc
    20821 gcttatgaac gcttgtcgac ggccctgacg gttgctcagg tttgcggcgt ccagcggtta
    20881 tgtaactact attcggcgcg acttacgccg ctccccggac ctgattcctc cagggaaagt
    20941 aatcatcggt tggcacaaat cacgcaatat gcccgccaac tggctagctc gccttctatt
    21001 atcgacaacc gatcgcgcca gcatctgaat gacgtcggtc ttactgtctg ggactgtgtg
    21061 atcattaacc aaatcattgg ttttattggc tttcaggcgc ggacaattgc gacatttcag
    21121 gcttatctcg ggcatccggt acgctggtta cccgggctgg cgatacaaaa ctacgccgac
    21181 gcgtcactgt ttgctgatga atcaatacgc tggcgaagca gctatgaggt ggaaaaacta
    21241 cctgaagagc acacaaaaag ttcaactgca gaactttgcc aactggccga aacacactct
    21301 ctccacccta tttcactttc ccttctcgaa aagttgttaa acagcacacg ggtcaatgca
    21361 cagccggata atcagcttgc ggcgttgtta tgcgcgcgta taaatggcag tcctgcttgt
    21421 ttttccacct gtatggattc atcaaatgaa tataaaaaaa tcagcaccct tctgcgcaag
    21481 ggcgaaaatg aaattaaccg atgggctgac cgtcattctg ttgagcgcgc taccgttcag
    21541 gcgatacaat ggctgacccg agcacccgat cgctttagcg ccgcccagtt cagcccttta
    21601 ctcgaacacg aaaaatcatc aacgcagatt attaatctgc tggtatggag cgggctgtgt
    21661 ggctggataa atcgcttaaa aatcgcgttg ggtgagacat attaaccttg ccgcgtcaga
    21721 cagattcgcg taaaactgtc agccgctcta atggccacca aaatagacaa ttatgtttca
    21781 ggacaacccg ctgctagcgc agcttaaaca gcaactgcat tcccagacgc cacgcgctga
    21841 aggggtggta aaagccacag aaaaaggctt tggcttcctg gaagtcgacg cacaaaaaag
    21901 ttatttcatt ccgccgccgc agatgaaaaa agtcatgcat ggcgaccgaa ttatcgcggt
    21961 gatccacagt gaaaaagaac gtgaatccgc agagccagaa gaactggttg aaccgttcct
    22021 gactcgtttc gtgggtaaag ttcagggcaa aaatgaccgt ctgaccatcg ttcctgatca
    22081 tccactttta aaagacgcca ttccttgccg cgcagcccgt ggcctgaacc acgagtttaa
    22141 agaaggcgac tgggcggttg ccgaaatgcg ccgtcatccg ctgaaaggcg atcgttcttt
    22201 ctatgctgaa ctgacacaat acatcacttt tggtgacgac cactttgtac cgtggtgggt
    22261 tacccttgcg cgccataatc tggaaaaaga agcaccagac ggcgtcgcta ccgaaatgct
    22321 cgatgaaggt ctggttcgtg aagatctgac ctcgctggat tttgtcacca tcgacagtgc
    22381 cagcactgaa gatatggatg acgccctttt cgctaaggcg ctgccggatg acaaacttca
    22441 gctgattgtg gcgattgccg atccaaccgc gtggattgct gaaggcagca agctggacaa
    22501 agccgcgaaa attcgcgcat tcaccaacta tctgcctggc ttcaacatcc ctatgctgcc
    22561 tcgcgagctt tctgacgatc tctgctcact gcgcgccaat gaagtccgcc cggtactggc
    22621 atgccgcatg acgctctccg ctgatggcac cactgaagat aatatcgaat tctttgccgc
    22681 caccatcgaa tccaaagcga agctggtgta tgaccaggtt tctgactggc tggaaaatac
    22741 cggtgactgg aagcctgaaa gtgaagcaat tgccgaacaa gtccgtttgc tagcgcaaat
    22801 ttgccaacgc cgcggcgagt ggcgtcataa ccacgcactg gtgtttaaag atcgcccgga
    22861 ttaccgcttt attctcggtg aaaaaggtga agtgctggat atcgtcgccg agcctcgtcg
    22921 cattgccaac cgtatcgtcg aagaagcgat gattgccgct aacatttgtg cggcccgcgt
    22981 actgcgcgat aagctcggtt ttggcatcta taacgtgcat atgggctttg atccggcgaa
    23041 tgccgacgcg ctggcagcgt tgctgaaaac gcacggtctg catgtcgatg ccgaagaagt
    23101 gctcacgctg gacggtttct gcaaactgcg tcgtgaactg gatgcgcaac caactggttt
    23161 cctcgacagc cgcattcgtc gcttccagtc atttgctgaa attagctctg aacccggtcc
    23221 tcactttggc ctcggtctgg aagcatacgc cacctggact tcgccgatcc gtaaatatgg
    23281 cgacatgatc aaccaccgtc tgctgaaagc ggttatcaaa ggcgaaactg cgacgcgtcc
    23341 gcaggatgaa atcactgtcc aaatggccga acgtcgccgt ctcaaccgga tggcagaacg
    23401 tgatgttggc gactggttat acgcacgctt cctgaaagat aaagccggga ccggcacccg
    23461 tttcgcggcg gaaattgtcg atatcagccg tggcggcatg cgtgtgcgtt tggttgataa
    23521 cggcgctatc gcctttattc cggcaccttt cttacacgct gtgcgcgatg aactggtttg
    23581 cagccaggaa aacggcactg tacaaattaa aggtgaaacg gtttacaaag taactgacgt
    23641 tattgacgtc accattgccg aagtccgcat ggaaacccgc agcattattg cgcgcccggt
    23701 cgcgtaatct cctttcacgg cccattcctc atggatgggc cgtttatttc cccgctctac
    23761 cttcgttata tccaggcaga ttttttaact gactttcgtt tgaaaactgg cgtttttcca
    23821 aaactggatt acaaataaat atataaaaaa caatgcgttt agccgccgtt aatttcatgt
    23881 aagtcatgtt gttcctttta tttaaccgcg gactccgctg ttaaccggag gatatgcatc
    23941 atgaaaaccg ttagggagtc cacaacgttg tacaactttc tcggatcgca caatccatac
    24001 tggcggttga cggaaagcag cgatgctttg cgcttttcta ccaccaaaac cacagaacct
    24061 gatcgtacat tgcagttatc tgccgaacag gctgctcgca tcagggaaat gacggtcatc
    24121 acctccagcc tgatgatgag tctgaccgtc gatgaaagcg atctttctgt gcatctggta
    24181 ggacgaaaaa tcaataaacg ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg
    24241 gcggttgctc gtgatttatc acacgggctt tcctttgctg agcaggtagt ttctgaagca
    24301 cattccgcaa tagtgattct cgacagccgg gggaatatcc aacgcttcaa tcggttatgt
    24361 gaagattaca cagggttgaa agaacacgac gtcattgggt aaagcgtgtt taaactgttt
    24421 atgagccgtc gtgaagctgc ggcatccagg cgcaataacc gtgtattttt tcgaagcggc
    24481 aatgcatatg aagtcgaact gtggatacca acacgtaaag gccagcggct gtttctgttt
    24541 cgcaataaat ttgtccacag cggcagtggc aaaaacgaga tttttttaat ctgttccggt
    24601 accgacatta ccgaagagcg ccgcactcag gagcgactgc gtattctggc aaataccgac
    24661 agtatcaccg gactgccgaa tcgtaacgca atgcaggagt taatcgatca cgctattaat
    24721 caggcagata acaataaagt tggggttgtg tatcttgatt tggataattt taaaaaggtc
    24781 aacgacgcct atgggcattt gtttggtgac cagttattac gcgacgtgtc attggctatt
    24841 ttaagctgtc tcgaacatga ccaggtgttg gcgcgtccag gtggcgatga gtttctggta
    24901 ctggcatcca atacctcaca aagcgcgctg gaagcaatgg catcacgaat tttgacccgc
    24961 ttacgcctcc cctttcgtat tggtttaatt gaagtttata ccagctgttc agtaggtatt
    25021 tcgctctctc ccgaacatgg ttcggacagc gcgactatta ttcgtcacgc cgacacagca
    25081 atgtacacag cgaaggaagg cggacgagga caattttgcg tttttactcc agaaatgaat
    25141 cagcgggtat ttgaatatct ctggctggat accaacttgc gtaaagcact ggaaaacgat
    25201 cagttggtta tcgaaaatca cctagcgtgg cgaagtgcgc agtctggaag cactagtacg
    25261 ttggcagtca cctgaacgtg ggttgattcc accgttggac ttcatttcct acgccgaaga
    25321 gtcagggcta attgtgcctt taggccgttg ggtgattctc gatgtcgtac gccaggtggc
    25381 aaagtggcgg gataaaggca taaacctgcg agtggcggta aatatttctg cacgtcagct
    25441 cgccgatcaa accattttca ccgccctgaa acaggttctc caggaactca attttgaata
    25501 ctgccctata gatgttgaac tgacagagag ttgtctgatt gagaatgatg aactggcact
    25561 gtctgttatt caacaattta gccaactagg tgcgcaagtg catctggatg attttggtac
    25621 cggctactct tcactttcgc aactggcgcg ctttccgatc gatgccatca aacttgacca
    25681 ggtttttgtt cgagatattc acaaacaacc tgtctcgcag tcactggtcc gggcgatcgt
    25741 cgctgtggcc caggcattga atcttcaggt gatcgccgaa ggtgtagaga gtgcaaagga
    25801 agatgctttt ttaaccaaga acgggatcaa tgagcggcaa ggatttttgt ttgccaaacc
    25861 gatgcccgcc gtcgccttcg aacgctggta taaacgctat ctgaagcgcg cataagtaga
    25921 tgaatatata tacttagcgc tatatcccgt cattatatat cgtattcctg gatgataatt
    25981 agccgttgct gttaaacact tctcttttag cggcaatttt tgcattattc attttccccc
    26041 ttttgctata aggatgtcct tgatgtctga atttgatgcc cagcgcgttg ccgaacgtat
    26101 tgatattgtg ttagatatcc ttgtggcagg cgattaccac tctgcgatcc ataatctcga
    26161 gatacttaaa gcagaactct tgcgccaggt tgctgaatct acgccagaca tcccgaagac
    26221 accgtgggag atttaacaat cgtgccttca ggttttccta tttgaggatg cccggcgcgt
    26281 cagtattttt ttatttagta ttataacgtt ataagaatta cagcgatgaa ttcccgacaa
    26341 caaactattc tacagatggt cattgaccag ggtcaggtta gcgtaaccga tctggcaaaa
    26401 gccactggag tttctgaagt taccattcgc caggatctca acaccctcga aaaactgagt
    26461 tacctccgcc gtgcacatgg ctttgcagtt tcgcttgata gtgatgacgt cgaaacccgt
    26521 atgatgagca actatacgct gaagcgtgaa ctcgccgagt ttgccgcgtc actggttcaa
    26581 ccgggcgaaa ccatctttat cgaaaatggc agcagcaatg ccctgctcgc ccggactctg
    26641 ggggagcaga agaaaaatgt cactatcatc acggtcagca gctacatcgc gcatttgctg
    26701 aaagacgcgc cttgtgaagt cattttgctc ggtggcgtgt accagaaaaa aagcgaaagt
    26761 atggttggcc ctttgacacg ccagtgcatc cagcaggtac atttcagcaa agcatttatt
    26821 ggtattgatg gctggcaacc tgaaactgga ttcaccggtc gcgatatgat gcgcaccgat
    26881 gtggtcaatg ccgtgctgga aaaagagtgc gaagcgatag tcctgactga cagctcgaaa
    26941 tttggtgctg tacattcata ctccatcggt cccgttgagc gattcaatcg cgtgattacc
    27001 gattcgaaaa tacgcgccag cgatctgatg catcttgagc acagtaaact caccgttcac
    27061 gtcgttgaca tttaaccatt tcccttctct acggatgatt tgcagtttgg caaatcatcc
    27121 gctctaagat gattcctggt tgataattaa gactatttac ctgttattaa cactctcaag
    27181 atataaaatt attatcagcg atataacagg aagtaattat cacctgcgtg atataaccct
    27241 gcgcgcgagc agatttcacg gaataatttc accaggctta ttcttagcta ttatagttat
    27301 agagagctta cttccgtgaa tcataaattc aggagagagt attatgtttg taacgagcaa
    27361 aaaaatgacc gcggctgttc tggcaattac tttggcaatg tctctgagtg cctgttctaa
    27421 ctggtctaaa cgggaccgca acaccgcaat cggcgcgggt gcaggggcat taggcggtgc
    27481 agtactgacc gatggcagta cgttgggtac attaggtggt gcagctgtcg gtggtgttat
    27541 tggtcaccag gtcggtaaat aatcgtaaat aaatgtcgat tatcgactcg caattataat
    27601 acgcttgatt ctgttcagta cgaaataact gaataataca cacagtaaaa tattcgaaag
    27661 ccacggatat atccgtggct ttttatgtta accgcctgcg agttttacct tcatcccttt
    27721 cgcttcaagt agtgatttta ataaatcacg cttatcgccc tggatttcaa taactccatc
    27781 tttaactgct ccgccgcagc cgcatttttt cttcagttcc gctgcaagtt ttgtcagttc
    27841 ggcatcatcg agatcgatac cggtaatcag gcaaacgccc ttacctttac gtccactggt
    27901 ctgacgctga atacgcaccg ctccatcgcc tttagggcgt actggggccg ctttgggctc
    27961 atcaatacgc ccggtttccg ttgagtagac cagacggctg ttggaatcac tcatcatgca
    28021 ctccgctgta aagaggcgtt gatcgctttc agtgtctgcg ctggatctac cgattgcgtt
    28081 accgggcgac caatcaccat ataatcaaca ccagccgcca acgcctgttc tggcgtcata
    28141 atgcggcgct ggtcaccagc ttcactcccc tgcggacgaa tgcccggtgt aaccagtttg
    28201 aactcctgac cgaatacctg tttaaagcgc acagcttcct gagcagaaca caccacacca
    28261 tcaaggtcac atttttgcgt cagtgctgcc agacgttctg cataatctgc aggtgacagt
    28321 gtcacgccaa gatcggccag gtcgctggct tccatgctgg tcaacactgt cacagcaatc
    28381 aaaagcggtg catctttgcc aaacggaacc agtgcctcac gcgctgcggt catcatacgc
    28441 gccccaccgg aggcatgaac attcaccatc cacacgccta agtcagctgc agcagcgaca
    28501 gcgtgcgctg cagtgttggg gatatcgtgg aatttcaggt caagaaagat atcaaaacca
    28561 cgctgttgaa gttcgcgcac aaactgtggc ccaaacaatg taaacatctc tttaccgacc
    28621 ttcagacgac aatcgcgtgg gtcgatcttg tcgacaaagg acagcgcgtc atcacaatta
    28681 tgataatcaa gggcaacaac cacaggagaa ttcgtaacgg cgcgggaaga agatgaagca
    28741 gttaacgtca tgaccagacc ttcttgatga tgggcaccgg gagtggcgcg aaacaggtaa
    28801 acggcgagca ttctacccgt gaaaataaaa aattaacagt tgcgattttt cccctagcag
    28861 gctttttctg ctccgtcgtt gccggagcac aatgggacat caataactag catgttgtaa
    28921 ctaaagtcgg atttttttaa aaattacagg ccatcaagac cgcgaatcgg tttaatagtt
    28981 gaccaggcac gacaagacgg acaatgccag tagagggtgt atgcagtaaa accacatttc
    29041 tggcagctat aacgaggctt actacgcacc ttctcgccaa ccatgtcacg cagcaccatc
    29101 agactctctt tggcgcgccc ttcttccgct tcatttaagt ggtaatccat tagcttatgg
    29161 aatacacgca tggtcggatg acgctgaagc tggcgtgtaa tatagacctg tgcagcctca
    29221 ctaccgtcgc gcgcttcgat gatatcagca agcatcaatt cagcatcggc accggtgttc
    29281 tcttccaccg cgcgttgcag gaattctgcc cattcggcag ttttacccaa ctgctgatag
    29341 caggtttgca acatttccag cgtttcgctg accagttctc tgtcctggga tatgacgcgt
    29401 tgcaaacttt cgacggcttt ggcgtattct cctttcgcca taaacacgcg tcccatcatg
    29461 atggatacgc gggcgctgtt tttatctgcc gccgcccctt tttttagcaa ggtcatggca
    29521 cgatcgagat cgtcgctggc catatgctgc agggctaact cacagtagaa atgggcaatt
    29581 tcgacgcgct gtttatcttt acccagcttc accaggcgtt cggcaacatc aattgctttc
    29641 tgccactcac tggtagcctg gtagatttgt agcaactgtt gcagcgcgcc aatgcggaag
    29701 tcagtttcat cggtcagctg attgaacatg tcttccgcgc ggtcgtataa cccggcggcc
    29761 atgtaatcac gccccagttg ttgaatcgcc aacagacgct gttcataggt cagcgaggcg
    29821 ctttccatta gggtctgatg gatgcgaata gcgcgatcaa cttcgccacg cgaacggaac
    29881 aggtttccga gcgtaaggtg ggcttcaacg gtgcctgtat cctctttaag catatcgaga
    29941 aacaggtcta ccgctttatc ctgttgatta ctaagcagga agttaacccc cgctacgtaa
    30001 tcacgcgaca agcggttggc ttcatcttgc ttgttttgtt gcgcacttct gcggcccata
    30061 taccagccat aggcagcggc tacaggcaaa agcagaaaca acaactccag catagaaagt
    30121 tattccttcg ccgctgacga gtgcggcact acagccacgt cagtcgcggg tgaaagctgg
    30181 ttttccagtc gctttatttt acgttcagcg cgcgccaggg aaacacgaac tcgcagccag
    30241 aacaggccac aaatcaacca accgatagca aaccccgcag caaacaatac cgccagcaat
    30301 gtggaaatac ggtactcccc ttgcgctaac agataattaa acgtcacctg ttgatcgttc
    30361 tgcgcaccca acgtgaccga aatcacgaag atcgctaaca ccagtaagaa aatgagtaaa
    30421 tatttcacat tacttcccgt tatgtggtta gagcgaataa attatggtca acttaccgca
    30481 atagcccttt aaagtaccat tttcgcgccg tctgcgaaat ggcatccacg cgcattgttg
    30541 atgatttaag gcctaaattc ggaaaatcaa ccagcgttaa ctttcttgtt ctcgttgtgc
    30601 aatttcgcga ttttcttccg caggtggtgt taatggccca caaattcgtt gcgcaagcca
    30661 cgttgccacc gccaccagcg cccacgaaat caacgtagct accaccagat cgcgtggcca
    30721 atgcatcccg agcagcaggc ggcttcccat gactcccgtt gcccagacca acaagatagc
    30781 aatggttaac gttcgccgac gcggccacag caaaccaacg gccagcagtg cccaactggc
    30841 agcaaacatc gtgtgaccgg aaggaaaggc aaaccccgtc tctttctgcc agtgtgaacg
    30901 caaatattgt ggaatatttt tctcttcagc caactgttct tttactagat ttccgcgttc
    30961 tgctcgcttt aaagtgtaga actcatcaac cggaatatga tgtgtttttt ccaaccagat
    31021 aacaaaaggt cgtggttcct gtactttgtc tttgatccag gatttaacgc cttgtccaac
    31081 aaggattgcc gccgccagaa tggcaaataa tacaaaggca gccttaatgc gaaaacgcag
    31141 acaccagaga aaccagccga acaaaatcaa atgtgtaatg acgccccagg gctgggtgac
    31201 agtttcagta acccaaaaag ccgcttttag tagccaactt tgttctccag gttgccaacg
    31261 ccagccagaa atccatacgg ctactggcat gacaagcaat agtgcagctc ccactgcggt
    31321 acgtcgggca atcgaacgca tggcctctcc tttttgataa gtcccacaat cataactgaa
    31381 aatgccagtt ccaagaaaaa ttgacagatt tgtgccattc cgtgaacgat cgacgcgtcg
    31441 tgattaggtg aaccccttct cgttatggca aaataagcca atacagaacc agcattatct
    31501 ggagaatttc atgcagctta aacgtgtggc agaagccaaa ctgccaaccc catggggcga
    31561 tttcctgatg gtgggatttg aagaactggc aaccggacac gatcatgtcg cgctagtcta
    31621 tggcgatatt tccgggcata ccccggtact tgcgcgcgtc cattccgaat gtctgaccgg
    31681 tgacgccctg ttcagcttgc gctgcgattg tggcttccag ctcgaagcgg cattgacgca
    31741 aattgccgag gaaggccgtg gtattttgct gtatcaccgt caggaaggtc gtaacattgg
    31801 tctgctgaat aaaatccgcg cttacgcact gcaggatcaa ggttacgata ccgtagaggc
    31861 taaccaccag ttaggcttcg ccgctgatga gcgcgacttc actctttgcg ctgatatgtt
    31921 caaactcctt ggcgtcaatg aagtccgctt gttaaccaat aacccgaaaa aagtcgaaat
    31981 tctgaccgaa gcagggatta atattgttga acgcgtacca ttgattgtag gtcgtaaccc
    32041 caataacgaa cattatctcg ataccaaagc cgagaaaatg ggccatttgc tgaacaaata
    32101 accctcttgc atcgtgtaat tcatttgctt gccggaagca aaataaccgg caagcaaata
    32161 cttgttactt caacatatta cgaatgacat aatgcaaaat gccgtcgttc tggtagtagg
    32221 tcaactccgt cgcggtgtcg atacgacaac ggcagggtac gacttcctgg ctaccatccg
    32281 cgcgcgtaag cgtcaccgga accgtcgcgc caggttgtag attttgcaga tcgccaatat
    32341 caatcttctc ttccccggtt agccctaacg ttttacgcgt cacgccttgc ggaaattcca
    32401 gcggcaggat gcccatgcca attaaattcg aacggtgaat tcgttcaaac gattcggcaa
    32461 tcaccacacg aattccaagc agacgaggtc cttttgccgc ccagtcacgg cttgagcctg
    32521 atccatactc tttcccggca atcaccgcca gcggcgtttg ctcctgctta tagcgcatcg
    32581 cagcatcata aatagagact acgtcgctgt caggtaaatg ccgcgtcatc cccccttcaa
    32641 cgccaggcac catttcatta cggatgcgaa tattggcgaa ggtgccgcgc atcatcactt
    32701 catggttacc acgccgcgaa ccgtaggagt taaagtcttt tcgctcaaca ccccgacctt
    32761 gtagatatcg acccgctggg ctgtcgggct taatactgcc cgccggagag atatggtcag
    32821 tggtgactga atcccccagc attgcgagga tccgcgcacc gtgaatatct tccactggtg
    32881 ctggtgttgc ctgcatttca tcaaagaaag gcgataagcg aatataggtt gagtcctcct
    32941 gccaaccgta ggtatcggat cgtgtgacgt taatttcctt ccactctgct gtgccttcaa
    33001 aaacttctgc gtactctttg cggaacattt ctgtggagac ttgttctacc gcacgggcaa
    33061 tttcttgtgc cgatggccag atatctttca gataaaccgg ctcgcctttg cgatcatggc
    33121 cgataggctc agaagccagg ttgatattca tatttcccgc cagcgcatag gcaaccacca
    33181 gcggtggcga ggccagccag ttagttttaa ccagcggatg gatacggcct tcaaagttac
    33241 ggttgccgga cagcaccgca ccgacggtta aatcgccttt tttgattgcc gtttcgatag
    33301 gatcgggcag cggcccagag ttaccaatac aggtggtaca accgtatccc acaaggttaa
    33361 accccagttc gtcgagatac ggtgtcagtt tcgcttttgc cagataatca gaaacgactt
    33421 tcgaacccgg tgccaacgac gctttgaccc acggttgccg cttgaggcca agagttacgg
    33481 cttttttcgc cagcaagcct gcggccatca gcacacttgg gttagaggtg ttggtgcacg
    33541 aggttatcgc agcaatgacc acagcgccat caggtaactg atactgatgt ccgttcataa
    33601 cataatcgac tggcaggcga tctttatgcg tggcattcac ttccagttcg ttactggcgg
    33661 caaatgcttt tggtacatcg ggcagtgcaa cgcgatcctg tgggcgtttt ggccctgcca
    33721 ggctcgcttc aacgtcattc atatccagtt ctaacatact ggtaaaaatc ggttcatcgc
    33781 ccgggttacg ccacatgccc tgcgctttgg catatttttc gaccaactcg acctgatctt
    33841 cgctgcgccc gcttaaacgc atgtaatcga gggttactgc atcgattggg aagaagccac
    33901 aggtggcacc atattctggc gacatattgg caatggtggc gcgatccgcc aacggtagtg
    33961 aatccagacc atcaccataa aattcgacga atttccccac cacgccatgt ttgcgcagca
    34021 tttgggtaac agtgagaacc aggtctgtgg cggtaatacc ttcacgtaat tttccggtaa
    34081 gtttaaagcc cactacgtcc gggataagca tggaaaccgg ctggcctaac atggcggctt
    34141 ctgcttcgat cccacccacg ccccacccca gcacgccaag gccgttgatc atagtggtgt
    34201 gcgagtcagt accaacgagt gtatccggat aagcaatcca ttcaccgtcc tgcaattcgc
    34261 tccacactgc tttgccgaga tattcgaggt taacctgatg gcaaatgcct gtgcctggcg
    34321 gcacgacgct aaaccgactg aacgcttgct ttccccattt caggaacaca taacgttcgt
    34381 ggttgcgctc catttccagg cgtacgtttt cttcaaatgc ctcatcatca ccaaaacgat
    34441 cgacggtcac cgagtggtca atgaccaggt cgaccggtga gagcgggtta acctttgcag
    34501 tatcgccacc gaggcgttta accgcttcgc gcattgccgc cagatcaaca acggcaggta
    34561 cgccggtaaa gtcctgcatc agcacccttg ccgggcggta ggcaatttca cggtcagcat
    34621 gggcattttt cagccatccc gccagtgcgt ggatatcctc ttcggtaact gagttaccat
    34681 cctgccagcg cagcaggttt tcgagcaaaa ctttgagtga cttgggtaga cgggtgatat
    34741 cgcccagtga tttagcagca agcggcaggc tgtagtagtg gtaagtttta tctttggcct
    34801 gcaacgtgtc cttactggct tctcgtaggg ttgacgacat agctcctcct taatgacagg
    34861 gttgcgaagc cctgattctc ttcagggtga atattaaaga taacacaaac atcgcgtaac
    34921 gatttgataa caacccaaat tgataaaaga ggagaaacct tgtggcaaag caaatgacaa
    34981 ccccgccgca gcggggtcaa ggatcagtga gtaatgatcc agaggagttc ggcccagaag
    35041 agcatcgaca gagaaaacgc gcccatccag gaccaatatt tcatatttgt gttgatcata
    35101 ggctacaccc ttaacggtta ttattttgtg ttgagataac tcaagctgtt attgctgcgg
    35161 tgtattaaaa gccctttaat tcattaaaaa tgggtaataa gaatgatgat aattattgtt
    35221 tcggctgatc atcttcagca aacagttcta taaaggcttg ctgctgcgat gttagttgcc
    35281 agtcgtctgg aataacgacg gaacattccc gtttcgtttc tggttcattg tcactatgct
    35341 ggcataatgg tgacgactcc agttgctcct gaccgaccat agttatcccc acactttcca
    35401 aatcagcagt gcgacaacca cccagaaaag tgcagaacca agaaaaaccg ccagccaggc
    35461 tttacgtttt aattcaggat cacggcgggg ttcctgattt ccagaaggca ttgctaacct
    35521 catataatcg ccatcactta tcagcaagac gttgaatgat tggtgtaatt aatcatacga
    35581 tgagacaaat cctaaagaaa ctttagtcaa aaatccagcg aatttacgaa tcgtctccag
    35641 tgaataattc aatcttttga cctgaaatag atatttgaaa actggaattt gcgaggcggg
    35701 taattagaca ctactttaca ggtaatttac cctataaata tgagggataa aaatattgtg
    35761 cacggtatag aaataagtaa caaaagagaa tatattccgg caccttcgct acataaaagg
    35821 tgccgaaaat aacgcaagaa attatttttc cggcagtttt atatctttaa acatgacctc
    35881 aatttcttca ttggagcgca atgcgacagc cgcatcaacg acatcacgcg ttaaatgcgg
    35941 tgcaaaacgc tgaatgaaat catacatata actgcgcaaa aaagtactac ggcgaaaacc
    36001 aattttggtt gtactgtggc tgaagatatc gtgagcatca acgcgcacaa ggtcgggatc
    36061 ggcgaccgga tccaccgcca tgctggcaat gacccctacc cccagcccta accggacgta
    36121 agttttaatg acgtcagcat ccgttgccgt gaaaacgata cgcggcgtta accctgcgcg
    36181 gttaaaggca gtatccagtt ctgaacgtcc ggtaaagccg aaggtatatg tcaccaacgg
    36241 atattgcgcc agttcttcaa tggtaatggc ttttttgcct gccagcgggt gatccggagt
    36301 gactacaata gcccgattcc agtggtagca cggtaacatc actaaatctt catacagatg
    36361 cagcgcttct gtggcgatag cgaaatcggc attgccttta gagacggcat cagcaatttg
    36421 tgtcggcggg ccctggtgca tatgcaaaga aacgcgggga taacgctcaa taaagccttt
    36481 gataacgttt ggtaacgcgt agcgtgcctg ggtatgcgtg gtggcgatat acagcgagcc
    36541 tttatccggc caggtgtgct caccggcaac cgattttatg gcatcgactt tcgacaggac
    36601 ttcgcgagcg atacgaatta tttcttgtcc tgctggcgtt acctgcgtca ggtgcttgcc
    36661 gcttcgggaa aaaatttgaa tgcctagctc gtcttccagc attctgactt gtttactgat
    36721 cccgggttgt gatgtgtaaa gtccttccgc tgttgatgag acattcaggt tatgattgac
    36781 cacctcaaca atatagcgaa gttgttgtaa tttcatgtta aaccatccac ttagacgcca
    36841 tcagaacatc gttttttcgt cacacctggc gggtgactaa tacgatttaa taataaaaaa
    36901 ggtgctaact ataaccacta tatcatttat agccttagtg tataggtgtg aagaaaaaat
    36961 aatatgcaat aaaaaagggc cgctttcgcg accctttgtt tataaaaacc tgacagaatt
    37021 aaaggttatt tttttccttc aacccatttg ccatcaacat aaaatgctga ccagccagtc
    37081 gcctttccgt ctttttccga agagacatat tgctgtttgg ttttacggct aaagcgaacc
    37141 atggtcttat taccttccgg atcctgctgt ggcgcatcgg ccagataacg cagtttttcc
    37201 ggcagacggt cgcggaagcg ataaagctct tccaccagtg gcgcacgcgt ttcacgcgat
    37261 ttcgggaaag tgttggcagc caggaacaca ccggcagcac cgtcacgcag cacgaaataa
    37321 gcatctgatt tttcgcacgg cagctcaggt aatggcaccg gatcttcttt cggtggtgcc
    37381 acttcgccgt tacgtaaaat cttacgtgtg tttttacact cttcgttggt gcaggccatg
    37441 tatttaccga aacgccccat tttcaggtgc atttcagagc cacatttttc acactcaacg
    37501 atcgggccgt cataaccttt aatgcggaat tcgccctctt cgatctcgta accgtcgcag
    37561 gttgggttat taccacagac atgcaactta cgtttcggat cgatgagata gctgtccatc
    37621 gccgtgccgc atttcgggca acgacgtttt gcgcgcagcg cgttggtttc agcatcttcg
    37681 ccttccagca cgttcagcac ttcgttttcc ggcaccaggt taatggtggt tttgcaacgc
    37741 tctttcggcg gcagcgcata gccagaacag ccaaggaata ccccggtact cgctgtgcga
    37801 atccccattt tgcgaccaca agtcgggcag tcaatgctgg tcagaaccat ctggttcggg
    37861 cgcataccac cctcttccgg atctttttca gctttatcta actgctgggt gaaatccgag
    37921 aagaagtgat ccagtacagc tttccactct gcttcgtgat ttgccacctg gtcgaggctg
    37981 ttttccatct gcgcggtaaa gtcgtagttc attaactcgc ggaaattttc ttcaaggcga
    38041 tcggtgacga tttcgcccat tttttccgca tagaaacgac gattttctac tcgcacatag
    38101 ccacgatcct gaatggtcga aatgatcgac gcataggtag acggacgacc gataccacgt
    38161 ttttccagtt ctttaaccag cgatgcttca ctgaaacggg ctggcggctt ggtaaagtgc
    38221 tgggctggtg ttagttcaac gagcgtcaga gcatcgcctt tatcgactgc cggtaagata
    38281 cgatcttcat cgcctttacg cagtgcaggc atcacttttg tccagccatc aaagcacaaa
    38341 atacgaccgc gtgctttcag gcggaaatcg cccgcaccaa cggtcagcgt cgtggagtca
    38401 tatttcgctg gggtcatctg gcaggcaacg aactgacgcc agattaactg gtacagtttc
    38461 tgcgcatctg cttccatatc cttcagcgat tccgccatca cattgacgtc agaaggacga
    38521 atcgcttcgt gcgcttcctg tgagttttct ttgctggcgt actgattcgg actttccggc
    38581 agatatttct taccaaaatt atcgctgata taaccgcgaa ccatatttac cgcgtcctga
    38641 ctcaggttag tggagtcggt acgcatgtaa gtgatatagc ctgcttcata caaacgctgc
    38701 gccatcatca tggttttttt cacgccaaaa ccaagacggg tgctggcagc ttgttgcagc
    38761 gtagaggtaa taaaaggagc gccaggttta ctggttgtcg gtttgtcttc acgttccagc
    38821 acgctgtagc gcgctttttc cagcagactt accgcagcct gagtttgttc tttgttgacc
    38881 ggacggaacg gtttgtcatt ctgatgagtc acctgcaacg ccaacgcttc accagatggc
    38941 gtagtcgtgc tggcatcgac ttcccagaac tcttccggta cgaacgcttt aatttcacgc
    39001 tcacgctcta ccaccaggcg gaccgccacc gactgcacac gaccggcaga cagaccacga
    39061 gcgatctttt tccacagcag cggcgaaacc atatacccca ccacgcggtc cataaagcga
    39121 cgcgcctgct gggcattaac acgatcaata ttcagctcac ccggtttgtt aaatgcctgg
    39181 cggatcgcgt ttttagtaat ttcgttaaac accactcggc tatagcgcgc atcatcacca
    39241 ccaatcactt cccgcaggtg ccatgcaatg gcttcccctt cgcggtcaag gtcggttgcg
    39301 agatagatgt ggtcggcttt ttcagccagt tgtttcagtt cagagacgac cttctcttta
    39361 ccaggcaaca cttcatagtg cgcctcccaa ttgtgccacg ggtcaacccc catacggttg
    39421 acgagagcgc cacgttcatc ctttttaggc tttttagccg tcttggtgga ggtagagtcg
    39481 gcactctttt tggcagctga gccactggtc ggcaaatcgc ggatgtgacc gacgctggat
    39541 ttcaccacgt agtcacaacc cagatacttg ttgatcgttt tggcttttgc cggggactca
    39601 acgatgacaa gagctttacc catattcacc ttacctaatt tgattcttcc aggaatgcgt
    39661 cgcacgttga taaaccttcc actggcaacg agttaccgat atggactcgc tctctgcgga
    39721 tatcaacccc ttgtcgtgac tctgttcacg aaaattcagg tgactgagtt aacaggtctt
    39781 ggcctacagg cgctatagca cgacggaatt ttggtcgaat gtcaagcaaa tctattgcca
    39841 gattgacgaa agcgtcacac tgtacctgat aaaatccgat acgcaacttt attagcatgc
    39901 aaaatccaaa gttgccagta aggcgctctt gccaccagcg tggtatattt gtctgcaagc
    39961 catgcctggc gtagactaat gtccttgttt aaggagtaaa taatgaacaa agagacacaa
    40021 cccatcgacc gcgaaacgct gttgaaagag gccaacaaaa tcattcgtga gcatgaggat
    40081 acgctcgcag ggattgaagc caccggcgtc acgcagcgta atggcgtgct ggtatttacc
    40141 ggcgactact ttttagatga acaaggattg ccaacagcca aaagcaccgc ggtgtttaat
    40201 atgttcaaac acctggccca tgtgctttca gaaaaatatc atctggttga ttaaagcaaa
    40261 acgcgaggtc ttagcctcgc gtttgtcttt tacatcaatg gcttttgacc ccgctgccac
    40321 cagcgtagca acaatcgatc ggcgctctct gccgcgctgc cggtgaatcg gtcaatgagt
    40381 cgtttacgct gcatatagcg tacattaacc acttcacggc cttccatcag gctaagaata
    40441 acttcatcac tggtgttgat ttcatcaacc aggcctttct ctaccgcctg ttgtccgtac
    40501 cagtgttcac ccgttgccac ctgttcaata tccagagacg gacgcatacg cttcacaaaa
    40561 tctttaaata actgatgcgt ttcgttcagc tcttcgcgga atttctcccg cccttcttcg
    40621 gtattttcac ccagcaaggt cagcgtgcgc ttatactgcc cggcggtgtg cagttcgata
    40681 tcaatatctt tgcttttcag gaagcggtta aagttgggca tttgcgccac caccccaatg
    40741 gaacccacaa tagcaaacgg tgcggaaaca attttgtccg ccacacaggc catcatgtaa
    40801 ccgccgctgg cagcgacttt gtctaccgta acagttaaag gaatgttttt atcacgcaga
    40861 cgctgcagct gcgaagccgc caacccgtaa ccatgcacca tgccaccagg gctttccaga
    40921 cggagcacaa cctgatcctg cggtttgaat gctgcgagta ccgccgttat ctcttcacgt
    40981 agcgagttca cttcatgggc gtccatgctg cctttaaaat ccagcaccca gacgcgaggt
    41041 ttactgtcag ttgccacctc gcccagtttg gctttcgctt ttgctgcttt cgcttcttgc
    41101 ttgtgctttt tcttctgcgc tttgtgccac tgtttttgct gatgtgagtc catcagcgcc
    41161 gcggccagtt cttctttcat ctccttatac tgttcgctga gattgttgac ccgtaactcg
    41221 ccacgctggc gtttattacg ttgagcaaca ttgacaataa tggcggcaat cgccgcaatc
    41281 gctagcacaa cggtaacgat tttagccaaa aacaaaccat attcagacaa caattccacg
    41341 agtccccacc ttggttaaac aacgctttgg ctcacagttt acaacaggca tgttaagccg
    41401 tctcgcagag atgttattgc agcgaaagta tgacaaagta tccattttgc ggtgttatcg
    41461 ccccaggctg attgaaatgc ccttctgttt caggcataaa gccccaaagt cataaagtac
    41521 actggcagcg cgacagacaa cgtcgttgag gagtagccat gcattaccag ccaaaacaag
    41581 atttactcaa tgatcgcatt atcctggtga cgggagccag cgatgggatt ggtcgtgaag
    41641 ccgcgatgac gtatgcacgc tatggtgcga cagtgattct gttgggccgt aatgaagaaa
    41701 aattacgtca ggtagccagc cacataaacg aagaaactgg gcgtcagcca cagtggttta
    41761 ttctcgattt gctgacctgc acgtccgaaa attgccaaca actggcacag cgcattgtcg
    41821 ttaattatcc gcgtctggat ggtgttttgc ataatgccgg attgctcggc gatgtttgcc
    41881 caatgagcga acaaaatccg caggtctggc aggacgtcat gcaggtcaac gttaatgcca
    41941 cctttatgct cacccaggca ctgcttcctt tattactcaa atcggacgcc ggttcactgg
    42001 tctttacttc atcaagcgtt ggacgtcagg gacgagccaa ctggggtgca tatgcagcgt
    42061 cgaaatttgc caccgaaggg atgatgcagg tactggccga tgaatatcag cagcgcctgc
    42121 gcgtcaactg cattaaccca ggcggtacgc gcaccgcaat gcgcgcaagc gccttcccga
    42181 ccgaagatcc acagaaactt aaaacacccg ctgatatcat gccgctctac ctctggctga
    42241 tgggcgatga cagccgccgt aaaaccggca tgacctttga cgcccaaccg ggccgtaaac
    42301 caggaatttc ccaatgagtg atgaacgcta ccaacagcgt cagcagcgag tgaaagaaaa
    42361 agtagatgct cgtgtggccc aggcccagga tgaacgcggt attatcatcg tctttaccgg
    42421 caatggaaaa ggcaaaacca ccgcggcatt tggtacggca acacgcgcag ttggtcacgg
    42481 aaaaaaagtg ggcgtcgtgc agtttattaa aggcacctgg cctaatggcg aacgcaatct
    42541 gctggagcca catggcgttg agtttcaggt gatggcaacg ggctttacct gggatacaca
    42601 aaaccgcgag tctgataccg ccgcctgccg cgaagtctgg caacatgcaa agcggatgct
    42661 tgctgattcc tcactggata tggttttgct tgatgaactg acgtatatgg tggcgtatga
    42721 ctatttgcca ctggaagaag tggtgcaggc gttaaatgaa cgtccacatc aacagacggt
    42781 gattatcacg ggtcgaggct gtcatcggga tattcttgaa ctggcggata cggtaagtga
    42841 gctgcgcccc atcagacatg cgtttgatgc cggtgttaag gcgcaaatag ggattgatta
    42901 ttaatctgta aaaatgccgg atatgacgtc cggcatactt tgattaaccg ttattacgcc
    42961 cgccagaacg acgactgccg ctcacctgac tgtgacgttt caccgcacga cgaatctgat
    43021 tagccttcat gcgacgacgg tctttttcta cagcgacttt agagctggtt tctggcggaa
    43081 gctccaccag ttcgcgcaga tagttagtct gggcgaggtc cagctccgtc cagccaccgc
    43141 gtggcagacc ttttggcagt gggatatcac cgtaacgaac acggatcagg cggctcacct
    43201 gcacaccaac cgcttcccac agacgacgaa cttcacggtt acggccttcg gtcagagtca
    43261 cgttgtacca ctggttgatc ccttcgccgc cgctgaactt gatggtttta aaagctgccg
    43321 gaccatcttc cagttgcacg ccacgactca aatcacgcag tttcgcgtcg tcaacctgac
    43381 caaatacacg cacggcgtat tcacgttcaa cttcacggct tggatgcatt aaacggtttg
    43441 ccagttcacc atcggtggtg aacagcagca gaccacaggt gttaacgtcc agacgaccta
    43501 cggcaatcca gcgtgcgcca cgcagctttg gcagacggtc aaacaccgtc ggacgccctt
    43561 ccggatcgtt acgggtacac aactcacctt ccggtttgta ataggccaga acgcgacaaa
    43621 tttgttcagc ggactcacgt accgaaatca gatgaccatc gatacgaatt ttcagcccag
    43681 gggtaacttc aacgcgatcg ccgagtttag caattttgcc atcaacactc acacgaccgg
    43741 cttcaataat agattcgatt tcacggcggg agccgtggcc agcgcgggcc agcactttct
    43801 gtaatttttc gctcatagag cttccttttg gtgtcgcctt cacaggcgtc gaacaggaga
    43861 atcaatggcg ccgtatagcg ccattttaag gccgcgtagt atacagagtt acgcacttat
    43921 aagaaaggct tcacatcacc tacgccttca cgcaccacga ccggcgtatc atcggtaaga
    43981 tcaataaccg ttgttggttt ctgcccgaga tagccgccgt gaataatcaa atctacctgt
    44041 ttttccagac ggtctttaat ttcttcggga tcggattcgg taaattcgct gcctggcagc
    44101 attagcgaag tggaaagcat cggttcgccc agtgcttcaa gcaacgcctg cgcaatcggg
    44161 ttagaaggca cacgcatacc gatggttttg cgtttctcct gcaacagacg gcgtggcact
    44221 tccttcgtcc ccttcaggat gaaggtatag ttgcccggcg tgttgttttt catcaaacga
    44281 aacgcaacgt tatccacgaa tgaataggtc gagagttcag aaagatcgcg acacatcagg
    44341 gtaaagttgt gaccgtccgg cagctggcga atacgacaaa tacgctccat ggcgttttta
    44401 tcttcaattt tacagccgag cgcatagccg gaatcagttg gatagacaat caccccgcct
    44461 ttacgcacga tctccaccgc ctggttgatc agacgttgct gcgggttatc aggatgaata
    44521 taaaaaaact ggctcataat tccctctctg tggtgttctg cggctgttcc catagctgcc
    44581 agacgccttc aacgcctgcg ggcagccaga gtttacgacc cagttcgatc cacgggcatg
    44641 gctgatgaaa atcagatcct tgtgatgccc ataaatgatg ctgacgcgca agtgtggcca
    44701 gctgggtgcg ttcattgggc gattgctggc actgcgcgac ttccatcgcg tcgccgtggt
    44761 gttcggcaaa atgcgcaacc aatcttttca gccatttagc actaagattg taccgcccag
    44821 gatgagctag caccgcctta ccgccagaat gatgaatgac atcaatagct tgttctattg
    44881 tacaccactg tggcggaacg tatccggttt tcccgcgcgc cagatacttt ttaaagacat
    44941 ctgccattga actggctttg ccgcactcca caaggaaacg cgcaaaatga ccgcgcgtca
    45001 ctgcgccgcc ctgcgccagt cgttgtgcgc cttccagcgc cccaggtatt tgcgcttttt
    45061 caagtctttc ggcaatttgc tgcgcccgct gattgcgccg ttctgtctgc tgcgcgagga
    45121 actcacacat tagcggatga gtaatatcaa tattcagccc gacaatatga atttcatgat
    45181 tttcccagac cgtggaaatt tccacgccgg gaataagatt cagcgccagt cccgaacgtg
    45241 aaatttcctc tcttgctggc gcgattgcag ctgtggtgtc atggtcggtg atcgccaggg
    45301 tgccgacgcg catctcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg
    45361 aagctgtggt atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc
    45421 actcccgttc tggataatgt tttttgcgcc gacatcataa cgggtctggc aaatattctg
    45481 aaatgagctg ttgacaatta atcatcgaac cagttaacta gtgcacaagt tcacgtaaaa
    45541 agggtatcga caatgaaagc aattttcgta ctgaaaggtt ggtggcgcac ttcctgaaac
    45601 gggcagtgta ttcaccatgc gtaaagcaat cagataccca gcccgcctaa tgagcgggct
    45661 tttttttgaa caaaattaga gaataacaat gcaaacacaa aaaccgactc tcgaactgct
    45721 aacctgcgaa ggcgcttatc gcgacaaccc gactgcgctt tttcaccagt tgtgtgggaa
    45781 tcgtccggca acgctgctgc tggaatccgc agatatcgac agcaaagatg atttaaaaag
    45841 cctgctgctg atagacagtg cgctgcgcat tacagcatta ggtgacaccg tcacaattca
    45901 ggcactttcc ggcaatggcg aagccctgct ggcactactg gataacgccc tgcctgcggg
    45961 tgtggaaagt gaacaatcac caaactgccg tgtgctgcgc ttcccccctg tcagtccact
    46021 gctggatgaa gacgcccgct tatgctccct ttcggttttt gacgctttcc gtttattgca
    46081 gaatctgttg aatgtaccga aggaagaacg agaagcaatg ttcttcggcg gcctgttctc
    46141 ttatgacctt gtggcgggat ttgaagattt accgcaactg tcagcggaaa ataactgccc
    46201 tgatttctgt ttttatctcg ctgaaacgct gatggtgatt gaccatcaga aaaaaagcac
    46261 ccgtattcag gccagcctgt ttgctccgaa tgaagaagaa aaacaacgtc tcactgctcg
    46321 cctgaacgaa ctacgtcagc aactgaccga agccgcgccg ccgctgccag tggtttccgt
    46381 gccgcatatg cgttgtgaat gtaatcagag cgatgaagag ttcggtggcg tagtgcgttt
    46441 gttgcaaaaa gcgattcgcg ctggagaaat tttccaggtg gtgccatctc gccgtttctc
    46501 tctgccctgc ccgtcaccgc tggcggccta ttacgtgctg aaaaagagta atcccagccc
    46561 gtacatgttt tttatgcagg ataatgattt caccctattt ggcgcgtcgc cggaaagctc
    46621 gctcaagtat gatgccacca gccgccagat tgagatctac ccgattgccg gaacacgccc
    46681 acgcggtcgt cgcgccgatg gttcactgga cagagatctc gacagccgta ttgaactgga
    46741 aatgcgtact gaccataaag agctgtctga acatctgatg ctggttgatc tcgcccgtaa
    46801 tgatctggca cgcatttgca cccctggcag ccgctacatc gccgatctca ccaaagttga
    46861 ccgttattcc tatgtgatgc acctcgtctc tcgcgtagtc ggcgaactgc gtcacgatct
    46921 tgacgccctg cacgcttatc gcgcctgtat gaatatgggg acgttaagcg gtgcgccgaa
    46981 agtacgcgct atgcagttaa ttgccgaggc ggaaggtcgt cgccgcggca gctacggcgg
    47041 cgcggtaggt tatttcaccg cgcatggcga tctcgacacc tgcattgtga tccgctcggc
    47101 gctggtggaa aacggtatcg ccaccgtgca agcgggtgct ggtgtagtcc ttgattctgt
    47161 tccgcagtcg gaagccgacg aaacccgtaa caaagcccgc gctgtactgc gcgctattgc
    47221 caccgcgcat catgcacagg agactttctg atggctgaca ttctgctgct cgataatatc
    47281 gactctttta cgtacaacct ggcagatcag ttgcgcagca atggtcataa cgtggtgatt
    47341 taccgcaacc atattccggc gcagacctta attgaacgcc tggcgacgat gagcaatccg
    47401 gtactgatgc tttctcctgg ccccggtgtg ccgagcgaag ccggttgtat gccggaactc
    47461 ctcacccgct tgcgtggcaa gctgcccatt attggcattt gcctcggaca tcaggcaatt
    47521 gtcgaagctt acgggggcta tgtcggtcag gcgggcgaaa ttcttcacgg taaagcgtcg
    47581 agcattgaac atgacggtca ggcgatgttt gccggattaa caaacccgct gccggtggcg
    47641 cgttatcact cgctggttgg cagtaacatt ccggccggtt taaccatcaa cgcccatttt
    47701 aatggcatgg tgatggcagt acgtcacgat gcggatcgcg tttgtggatt ccagttccat
    47761 ccggaatcca ttctcaccac ccagggcgct cgcctgctgg aacaaacgct ggcctgggcg
    47821 cagcagaaac tagagccagc caacacgctg caaccgattc tggaaaaact gtatcaggcg
    47881 cagacgctta gccaacaaga aagccaccag ctgttttcag cggtggtgcg tggcgagctg
    47941 aagccggaac aactggcggc ggcgctggtg agcatgaaaa ttcgcggtga gcacccgaac
    48001 gagatcgccg gggcagcaac cgcgctactg gaaaacgcag cgccgttccc gcgcccggat
    48061 tatctgtttg ctgatatcgt cggtactggc ggtgacggca gcaacagtat caatatttct
    48121 accgccagtg cgtttgtcgc cgcggcctgt gggctgaaag tggcgaaaca cggtaaccgc
    48181 agcgtctcca gtaaatccgg ctcgtcggat ctgctggcgg cattcggtat taatcttgat
    48241 atgaacgcca ataaatcgcg ccaggcgctg gacgagttag gcgtatgttt cctctttgcg
    48301 ccgaaatatc acaccggatt ccgccacgcg atgccggttc gccagcaact gaaaacccgc
    48361 accctgttca atgtgctggg gccattgatt aacccggcgc atccgccgct ggcgttaatt
    48421 ggtgtttata gtccggaact ggtgctgccg attgccgaaa ccttgcgcgt gctggggtat
    48481 caacgcgcgg cggtggtgca cagcggcggg atggatgaag tttcattaca cgcgccgaca
    48541 atcgttgccg agctgcatga cggcaaaatt aagagctatc aattgaccgc tgaagatttt
    48601 ggcctgactc cctaccacca ggagcaactg gcaggcggaa cacctgaaga aaaccgtgac
    48661 attttaacac gcttgttaca aggtaaaggc gacgccgccc atgaagcagc cgtcgctgcg
    48721 aacgtcgcca tgttaatgcg cctgcatggc catgaagatc tgcaagccaa tgcgcaaacc
    48781 gttcttgagg tactgcgcag tggttccgct tacgacagag ttaccgcgct ggcggcacga
    48841 gggtaaatga tgcaaaccgt tttagcgaaa atcgtcgcag acaaggcgat ttgggtagaa
    48901 acccgcaaac agcagcaacc gctggccagt tttcagaatg aggttcagcc gagcacacga
    48961 catttttatg atgcgctaca gggtgcacgc acggcgttta ttctggagtg taaaaaagcg
    49021 tcgccgtcaa aaggcgtgat ccgtgatgat ttcgatccgg cacgcattgc cgccatttat
    49081 aaacattacg cttcggcaat ttcggtgctg actgatgaga aatattttca ggggagcttt
    49141 gatttcctcc tcatcgtcag ccaaatcgcc ccgcagccga ttttatgtaa agacttcatt
    49201 atcgaccctt accagatcta tctggcgcgc tattaccagg ccgatgcctg cttattaatg
    49261 ctttcagtac tggatgacga ccaatatcgc cagcttgcag ccgtcgctca cagtctggag
    49321 atgggggtgc tgaccgaagt cagtaatgaa gaggaactgg agcgcgccat tgcattaggg
    49381 gcaaaggtcg ttggcatcaa caaccgcgat ctgcgtgatt tgtcgattga tctcaaccgt
    49441 acccgcgagc ttgcgccgaa actggggcac aacgtaacgg taatcagcga atccggcatc
    49501 aatacttacg ctcaggtgcg cgagttaagc cacttcgcta acggttttct gattggttcg
    49561 gcgttgatgg cccatgacga tttgcacgcc gccgtgcgcc gggtgttgct gggtgagaat
    49621 aaagtatgtg gcctgacgcg tgggcaagat gctaaagcag cttatgacgc gggcgcgatt
    49681 tacggtgggt tgatttttgt tgcgacatca ccgcgttgcg tcaacgttga acaggcgcag
    49741 gaagtgatgg ctgcggcacc gttgcagtat gttggcgtgt tccgcaatca cgatattgcc
    49801 gatgtggtgg acaaagctaa ggtgttatcg ctggcggcag tgcaactgca tggtaatgaa
    49861 gatcagctgt atatcgatac gctgcgtgaa gctctgccag cacatgttgc catctggaaa
    49921 gcattaagcg tcggtgaaac cctgcccgcc cgcgagtttc agcacgttga taaatatgtt
    49981 ttagacaacg gccagggtgg aagcgggcaa cgttttgact ggtcactatt aaatggtcaa
    50041 tcgcttggca acgttctgct ggcggggggc ttaggcgcag ataactgcgt ggaagcggca
    50101 caaaccggct gcgccggact tgattttaat tctgctgtag agtcgcaacc gggcatcaaa
    50161 gacgcacgtc ttttggcctc ggttttccag acgctgcgcg catattaagg aaaggaacaa
    50221 tgacaacatt acttaacccc tattttggtg agtttggcgg catgtacgtg ccacaaatcc
    50281 tgatgcctgc tctgcgccag ctggaagaag cttttgtcag cgcgcaaaaa gatcctgaat
    50341 ttcaagccca gttcaacgac ctgctgaaaa actatgccgg gcgtccaacc gcactgacca
    50401 aatgccagaa cattacagcc gggacgaaca ccacgctgta tctcaagcgt gaagatttgc
    50461 tgcacggcgg cgcacataaa accaaccagg tgctggggca ggcgttgctg gcgaagcgga
    50521 tgggtaaaac cgaaatcatc gccgaaaccg gggccggtca gcatggcgtg gcgtcggccc
    50581 ttgccagcgc cctgctcggc ctgaaatgcc gtatttatat gggtgccaaa gacgttgaac
    50641 gccagtcgcc taacgttttt cgtatgcgct taatgggtgc ggaagtgatc ccggtacata
    50701 gcggttccgc cacgctgaaa gatgcctgta atgaggcgct acgcgactgg tccggcagtt
    50761 atgaaactgc gcactatatg ctgggcaccg cggctggccc gcatccttat ccgaccattg
    50821 tccgtgagtt tcagcggatg attggcgaag aaaccaaagc gcagattctg gaaagagaag
    50881 gtcgcctgcc ggatgccgtt atcgcctgtg ttggcggcgg ttcgaatgcc atcggcatgt
    50941 ttgctgattt catcaatgaa accaacgtcg gcctgattgg tgtggagcct ggtggtcacg
    51001 gtatcgaaac tggcgagcac ggcgcaccgc taaaacatgg tcgcgtgggc atttatttcg
    51061 gtatgaaagc gccgatgatg caaaccgaag acgggcagat tgaagaatct tactccatct
    51121 ccgccggact ggatttcccg tctgtcggtc cacaacacgc gtatcttaac agcactggac
    51181 gcgctgatta cgtgtctatt accgatgatg aagcccttga agccttcaaa acgctgtgcc
    51241 tgcacgaagg gatcatcccg gcgctggaat cctcccacgc cctggcccat gcgctgaaaa
    51301 tgatgcgcga aaacccggat aaagagcagc tactggtggt taacctttcc ggtcgcggcg
    51361 ataaagacat cttcaccgtt cacgatattt tgaaagcacg aggggaaatc tgatggaacg
    51421 ctacgaatct ctgtttgccc agttgaagga gcgcaaagaa ggcgcattcg ttcctttcgt
    51481 cacgctcggt gatccgggca ttgagcagtc attgaaaatt atcgatacgc taattgaagc
    51541 cggtgctgac gcgctggagt taggtatccc cttctccgac ccactggcgg atggcccgac
    51601 cattcaaaac gccactctgc gcgcctttgc ggcaggtgtg actccggcac aatgttttga
    51661 aatgctggca ctgattcgcc agaaacaccc gaccattccc attggcctgt tgatgtatgc
    51721 caatctggtg tttaacaaag gcattgatga gttttatgcc cagtgcgaga aagtcggcgt
    51781 cgattcggtg ctggttgccg atgtgccagt tgaagagtcc gcgcccttcc gccaggccgc
    51841 gttgcgccat aatgtcgcac ctatcttcat ctgcccgcca aatgccgatg acgacctgct
    51901 gcgccagata gcctcttacg gtcgtggtta cacatatttg ctgtcacgag cgggcgtgac
    51961 cggcgcagaa aaccgcgccg cgttacccct caatcatctg gttgcgaagc tgaaagagta
    52021 caacgctgca cctccattgc aggggtttgg tatttccgcc ccggatcagg taaaagcagc
    52081 gattgatgca ggagctgcgg gcgcgatttc tggttcggcc attgttaaaa tcatcgagca
    52141 acatattaat gagccagaga aaatgctggc ggcactgaaa gtttttgtac aaccgatgaa
    52201 agcggcgacg cgcagttaat cccacagccg ccagttccgc tggcggcatt ttaactttct
    52261 ttaatgaagc cggaaaaatc ctaaattcat ttaatattta tctttttacc gtttcgctta
    52321 ccccggtcga acgtcaactt acgtcatttt tccgcccaac agtaatataa tcaaacaaat
    52381 taatcccgca acataacacc agtaaaatca ataattttct ctaagtcaca tattcctcag
    52441 gtaattgtta atatatccag aatgttcctc aaaatatatt ttccctctat cttctcgttg
    52501 cgcttaattt gactaattct cattagcgac taattttaat gagtgtcgac acacaacact
    52561 catattaatg aaacaatgca acgcaacggg agaaataaca tggccgaaca tcgtggtagt
    52621 tcaggaaatt tcgccgaaga ccgtgagaag gcatccgacg caggccgtaa aggcggtcag
    52681 catagcggcg gtaattttaa aaatgatccg caacgcgcat ctgaagcggg taaaaaaggc
    52741 ggtcaacaaa gcggtggtaa taaatcaggc aaatcctgat tcaccgttaa ttattaatat
    52801 cagctgataa agataattgc atctgcgggc cgtatggctc gcagtgattt cactggagaa
    52861 aaatatgaat atgaagacca ttgaagatgt atttattcaa ctgctttcag atacctacag
    52921 cgcagaaaaa caattaaccc gggcactggc aaaactcgca agagcaacat caaatgaaaa
    52981 attaagtcag gcttttcatg cgcacctcga ggaaactcat ggacagattg aacgaattga
    53041 tcaagttgtg gaatcagaat caaatctgaa aattaagcgc atgaaatgtg tggcaatgga
    53101 aggtcttatt gaagaagcta atgaggtcat cgaaagtacc gagaaaaacg aagtgcgtga
    53161 tgccgcactg attgccgcag cacagaaagt cgagcattat gagattgcca gttacgggac
    53221 attagcgacg ctggctgaac aattaggtta ccgtaaagca gcgaagcttc tgaaagaaac
    53281 cctggaagaa gaaaaggcca ccgacatcaa actgactgat ctggccctta ataacgtaaa
    53341 taagaaagcc gaaaataaag cctgaaatat gaattttaac ttttagtcat tttataaaga
    53401 ggacattttc atgaatcgta ttgaacatta tcatgactgg ttacgtgacg cccacgcaat
    53461 ggaaaagcaa gccgaatcta tgcttgaatc catggccagc cgtatagata attatcctga
    53521 actacgcgct cgtattgaac aacatcttag tgaaaccaaa aaccagattg ttcaactgga
    53581 aactattctt gatcgtaatg acatttcacg ttcagtcatt aaagattcca tgagtaaaat
    53641 ggctgcgctt gggcagtcaa tcggtggtat attcccttct gatgaaatag ttaaaggctc
    53701 tattggcgga tatgtcttcg agcaatttga aatcacctgt tacacctcac tattagcagc
    53761 agcaaaaaat gccggtgata cagtctcaat tccgatcatc gaagcgattt taaatgagga
    53821 aaagcaaatg gccgactggc tgattcagca tattccgcaa acaactgaga aatttttaat
    53881 tcgttctgaa actgatggcg tagaagcgaa gaaataatac cctttatacc atgtccttat
    53941 tgaccccgta tattacgggg tcgtttttgt gcggaattaa aaacgatatc ctgctgagaa
    54001 cataaacacc cacggatcga ggcgtacgct atcgtgttgc tgtgcaccgc ctagcttata
    54061 attggcggtg gtatcgatat ccatgtacca cactgacatg ttaaccaacc agtcacggtt
    54121 aatcagataa tcaaccccca cctgcccggc agctccccag gaatctttca gactgagatc
    54181 ggaaagcccc gcctctttgc catgatcgtt aaatccatta tcaaagaagg tggtgtagtt
    54241 aataccggcc ccaacgtaag gacggaattt gctgctggca tcaccaaaat accactgcgc
    54301 catcagtgtt ggcggcagat ggtgaacggt tgcaatatcg ccggtcgccc gggtgccgat
    54361 tttatggcgg aacggcgtcg ctgccagtaa ttccacacca atgttgtcgg tcgccatata
    54421 agtaaacgtc aagcccagtt gagtgttatt ggtcacgctg aatccaccca gacttcctaa
    54481 cgtaccacca gcaccttctg ttggacgtac ggttgcagaa cctgcacgca taaaaaattc
    54541 gcctgcttca tgcgcaaagg cactgccaga aagaagagtt gttactgcca aagccgccac
    54601 tgttaacttt ttcatatccg ctccgtcgtt atggttataa aaacgtgacg aatataccta
    54661 cattggagta acaaatgatc ctacatagat cacattacat atgataattc accatttatt
    54721 gatctggatt aatttagaag ttgcattgag aatcctggtt aatgtgattt aaatcaattt
    54781 ttaaatattt tctctacgca aatattaact cgatcgccac ttgccgcggt ttgcccacgc
    54841 ctgaaggcca gcaattacct ccgctttaca aagatatgct tttccataca agacctgatg
    54901 ctgccacccg aggcttaacc agggtacaat tgcccgctaa ttaacacctg cataaaactc
    54961 aaggagagtg catgtctatc acggcgcagt ccgtataccg tgacaccgga aatttcttcc
    55021 gtaatcaatt tatgaccatt ctgttggtat cgttgctatg tgcgtttatc acagtggtgt
    55081 tagggcatgt tttctcaccc agtgatgcac agcttgcgca gctcaatgac ggcgtgcccg
    55141 ttagcggcag tagtgggttg ttcgacctgg ttcagaatat gtcaccggaa cagcaacaaa
    55201 ttttgctgca ggcttcagcg gcgtccactt tttcagaatt aatcggtaac gccattctcg
    55261 ccggaggcgt aatattaatt atccagctgg tgtctgcggg tcagagagtc agtgcgctgc
    55321 gagctattgg tgccagtgcg ccgatattgc ccaagttatt tattctgatt tttctgacta
    55381 cccttttagt acagattggc atcatgctgg tggtcgttcc gggaattatc atggccattt
    55441 tactggccct ggcaccggtg atgctggtac aggacaaaat gggcattttt gcctcgatgc
    55501 gtagcagtat gcggctgact tgggcgaata tgcgtctggt ggcacccgca gtactgagct
    55561 ggttgctggc aaaaacactg ttgctgcttt ttgcctcttc ttttgccgca ttaaccccgg
    55621 aaattggtgc cgtactggcg aacaccttga gcaacctgat ttctgccata ttgctcatct
    55681 atctgttccg cctgtatatg ttgattcgcc aataacctta agcatttgat tacggaatcg
    55741 taaaatgaag cagtttcttg attttttacc gctggttgtc tttttcgcgt tttacaagat
    55801 ttatgacatc tatgcggcta ctgcagcgct gatcgtcgcc acggcgattg tgcttatcta
    55861 tagctgggtt cgctttcgta aggttgagaa gatggccctg atcacttttg ttctggtggt
    55921 cgtcttcggt ggcttgacgc tgttcttcca caatgatgag tttattaaat ggaaggtgac
    55981 agtcatttat gctctgtttg cgggagccct gttagtcagc caatgggtga tgaaaaagcc
    56041 gctaattcag cggatgctga gtaaagaact cacgctgccg caaccggtat ggtcgaagct
    56101 aaatctggcc tgggctgttt tctttatcct ttgcggtctg gcaaacatct acatcgcatt
    56161 ctggctgccg caaaatattt gggtcaactt taaagtcttt ggcctgaccg cccttacctt
    56221 aatcttcaca ttgttaagcg gtatctatat ctaccgccac atgccgcagg aagataaatc
    56281 ctaaccagac tgccagtcct tcggggctgg cgctgtatct cctctgttct cctcaggaaa
    56341 atcatagtag catcgcgcct gtgattttcc ttttaagtcg gttttaccat gtctacaaca
    56401 cataacgtcc ctcagggcga tcttgtttta cgtactttag ccatgcccgc cgataccaat
    56461 gccaatggtg acatctttgg tggttggtta atgtcacaaa tggatattgg cggcgctatt
    56521 ctggcaaaag aaattgccca cggtcgcgta gtgactgtgc gggttgaagg aatgactttc
    56581 ttacggccgg ttgcggtcgg cgatgtggtg tgctgctatg cacgctgtgt ccagaaaggg
    56641 acgacatcgg tcagcattaa tattgaagta tgggtgaaaa aagtagcgtc tgaaccaatc
    56701 gggcaacgct ataaagcgac agaagcatta tttaagtatg tagcggttga tcctgaagga
    56761 aaacctcgcg ccttacctgt tgagtaatag tcaaaagcct ccggtcggag gcttttgact
    56821 ttctgcttac tgaatttcgg tggtgccgtt aattttaaac aggatattca ccacaatccc
    56881 actgcctggc ttacccggct cataacgcca tctgcgcatc gcatttttca cctcacgctc
    56941 aaacatgttc gcaggcttgg ctgagaggat ttgtacgtta tccacgcgac catccggcgt
    57001 gacgtcaaat ttaactttaa cctgcccttc aatgcgcaat gcctgtgctc gtgccggata
    57061 ctgcggctga ttacggctta atgcgcgtgg tcctgaagcc acactggtaa ccggcttgct
    57121 ggttgcagcc gttgccgtac ttgatgtggg gcgcgcgggt gccgtatttt caaacggtga
    57181 tgccggacgc gactctacgg gtttgacatc gcgttttggc tgctcctgta cctttttcac
    57241 cggcttcggt tttggcttcg gtttcggctt cggcttttca atgaccaccg gtgcttcttt
    57301 tggcggttcg gggatcggct caggttccgg ttctggctct accaccggct ccggtggcgg
    57361 ctgaacggct tgtggcggtt cgagatcagc aggcgcaacc atcgtgacag aaattggttg
    57421 cgcaggcgca ggtagttcaa taacctgatg taccgaggta tagagcagcc ccgccacaac
    57481 agcaccatga atgcagaccg aaagtaacgt cggccagggg aagcggcgag gtaaatcaag
    57541 ggttattgaa gtcataatca tttcagtaga aaaactaggc ctcgatttta aatgcaaata
    57601 gcaatcatat tcaataaggc aagacgatca ggatcagtga aaatgtcatt tttggcgtaa
    57661 ttacccttac tttatgcatg accttaacaa agacatcatc tttccgttgc agatcttcgc
    57721 cctttcaaat aacgtacttt acaactttcc cgaacaagga gttgtgcccg tgttgtatgt
    57781 tatttacgct caagacaaag ctgatagcct cgaaaaacgc ctttccgttc gtccggcaca
    57841 tttagcacgt ttacagttac tgcatgatga aggtcgtttg ttgacagctg gtccaatgcc
    57901 agcggtagac agtaacgatc caggggctgc gggatttact ggctcaaccg ttattgctga
    57961 atttgaatcg ctggaagcag ctcaggcctg ggccgatgct gacccgtacg tagcggcagg
    58021 agtctatgaa cacgtttcag tgaaaccatt taagaaagtg ttctgag
//
LOCUS       DAJVRP010000012        58048 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVRP010000012 DAJVRP010000000
VERSION     DAJVRP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.012"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(24..278)
                     /locus_tag="OQN12_000724"
     CDS             complement(24..278)
                     /locus_tag="OQN12_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS1653177.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OQN12_000725"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OQN12_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1653178.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OQN12_000726"
     CDS             1228..2694
                     /locus_tag="OQN12_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1653179.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGADWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OQN12_000727"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OQN12_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1653180.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OQN12_000728"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OQN12_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1653181.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OQN12_000729"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OQN12_000729"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1653182.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OQN12_000730"
     CDS             5709..7265
                     /locus_tag="OQN12_000730"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1653183.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OQN12_000731"
     CDS             7347..9161
                     /locus_tag="OQN12_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1653184.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OQN12_000732"
     CDS             9162..10256
                     /locus_tag="OQN12_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1653185.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OQN12_000733"
     CDS             10256..11281
                     /locus_tag="OQN12_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1653186.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OQN12_000734"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OQN12_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1653187.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OQN12_000735"
     CDS             complement(12876..13220)
                     /locus_tag="OQN12_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653188.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OQN12_000736"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OQN12_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCS1653189.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OQN12_000737"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OQN12_000737"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1653190.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OQN12_000738"
     CDS             15616..17376
                     /locus_tag="OQN12_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1653191.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OQN12_000739"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OQN12_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1653192.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OQN12_000740"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OQN12_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1653193.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OQN12_000741"
     CDS             19113..19340
                     /locus_tag="OQN12_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1653194.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OQN12_000742"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OQN12_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1653195.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OQN12_000743"
     tRNA            21195..21271
                     /locus_tag="OQN12_000743"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OQN12_000744"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OQN12_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCS1653196.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OQN12_000745"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OQN12_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1653197.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OQN12_000746"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OQN12_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1653198.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OQN12_000747"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OQN12_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1653199.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OQN12_000748"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OQN12_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1653200.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OQN12_000749"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OQN12_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1653201.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OQN12_000750"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OQN12_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1653202.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OQN12_000751"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OQN12_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1653203.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OQN12_000752"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OQN12_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1653204.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OQN12_000753"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OQN12_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1653205.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OQN12_000754"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OQN12_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1653206.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OQN12_000755"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OQN12_000755"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1653207.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OQN12_000756"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OQN12_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1653208.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OQN12_000757"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OQN12_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1653209.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OQN12_000758"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OQN12_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1653210.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OQN12_000759"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OQN12_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1653211.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OQN12_000760"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OQN12_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1653212.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OQN12_000761"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OQN12_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1653213.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39347)
                     /locus_tag="OQN12_000762"
     CDS             complement(38328..39347)
                     /locus_tag="OQN12_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCS1653214.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(39581..39940)
                     /locus_tag="OQN12_000763"
                     /pseudo
     CDS             complement(39581..39940)
                     /locus_tag="OQN12_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="OQN12_000764"
     CDS             complement(40158..41801)
                     /gene="yojI"
                     /locus_tag="OQN12_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1653215.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="OQN12_000765"
     CDS             complement(41877..42527)
                     /gene="alkB"
                     /locus_tag="OQN12_000765"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS1653216.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42527..43591)
                     /gene="ada"
                     /locus_tag="OQN12_000766"
     CDS             complement(42527..43591)
                     /gene="ada"
                     /locus_tag="OQN12_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1653217.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="OQN12_000767"
     CDS             complement(43665..44720)
                     /gene="apbE"
                     /locus_tag="OQN12_000767"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1653218.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="OQN12_000768"
     CDS             complement(44832..45953)
                     /gene="ompC"
                     /locus_tag="OQN12_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1653219.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46691..49363
                     /gene="rcsD"
                     /locus_tag="OQN12_000769"
     CDS             46691..49363
                     /gene="rcsD"
                     /locus_tag="OQN12_000769"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1653220.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49380..50030
                     /gene="rcsB"
                     /locus_tag="OQN12_000770"
     CDS             49380..50030
                     /gene="rcsB"
                     /locus_tag="OQN12_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1653221.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="OQN12_000771"
     CDS             complement(50230..53079)
                     /gene="rcsC"
                     /locus_tag="OQN12_000771"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1653222.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53354..54130)
                     /locus_tag="OQN12_000772"
     CDS             complement(53354..54130)
                     /locus_tag="OQN12_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCS1653223.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54135..55784)
                     /locus_tag="OQN12_000773"
     CDS             complement(54135..55784)
                     /locus_tag="OQN12_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1653224.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55785..56344)
                     /locus_tag="OQN12_000774"
                     /pseudo
     CDS             complement(55785..56344)
                     /locus_tag="OQN12_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56503..>58048)
                     /locus_tag="OQN12_000775"
     CDS             complement(56503..>58048)
                     /locus_tag="OQN12_000775"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1653225.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMPERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14197 a        15659 c        14266 g        13926 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcgccgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagagta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgaact ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatcttt tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgtgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcggcgtac tatctgctgc ttgcggtttg
    38401 tcgtatttca gccccagcac ttcgctggtg tactgcaact cgcgttctgt tgccgctaca
    38461 tcaccgttca gcttgcgtcc ataagacgga acgatcgctt tcaacgtagc ctgccattgc
    38521 gggctggaaa cgcgatcgcc aaatactttt tccagtagat tcaacataat cggcgcggcg
    38581 gttgacgccc ctggcgatgc ccccagcagc gccgcaatgg tcccttgctg gtcactgacc
    38641 acttcggtac ccagacgcag tacgccacct ttctcggcat cacgcttgat aatctgcaca
    38701 cgctgccccg cttgccacaa acgccagtcc tcttttttcg cttgcggata gtactctttc
    38761 aacgcttcaa aacgatcctc ttcactcaac atcacctgac tcaccagata tttcaccaga
    38821 tcgaaattat ccagcccgac gtgcatcatc ggcatcacgt tagaggtggt ggtggaactc
    38881 atcagatccc acaatgaacc gtttttgagg aatttggttg agaaggtggc aaatggccca
    38941 aacagcacta cgcgtttacc gtccagaaca cgggtatcga tatgcggaac cgacatcggt
    39001 ggtgcgccaa cggatgcttt accgtaaact ttcgccagat ggcgattaac cacgtctgga
    39061 ttttccgaaa caaggaactg tccgcccacc gggaaaccgg cgtagtcttt cgcttccgga
    39121 atccccgatt cctgtaacag cttcagcgcc gcaccgcccg cgccgataaa gacaaatttg
    39181 gcgcggatgt tctgtgcagt gccgtttttc agatcggcaa cggtaacggt ccaggtgtta
    39241 tcgtcattac gctttagggc gcggacttcg ctgctgagtt gcagcgagaa gttagatttc
    39301 ttctgtaagg aagcaattaa ctggcgggtg atctcaccat agttcacatc agtgccaatt
    39361 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
    39421 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
    39481 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
    39541 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
    39601 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt
    39661 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttctagccgt
    39721 tcgaactctc ctgcgcgccc ccctccaggc gctccaccat ggtcatcgac cattcaggct
    39781 ccagctcgcg tcaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
    39841 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
    39901 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
    39961 agtcgcttta ttgcaggtga gatttgcgcg gcatcaacgg taacacttaa gtaacaacat
    40021 ttaaataatg tttataaatt atattctaat tttagaaaca ttataatttc gttgattaat
    40081 tatagggtta ttagaaatga agggattttt agcatattga cctgacggca gcagtccgcc
    40141 aggtcagcgt cgtaatatta tgccgtccgg gcaacggcat cacgcgaagc ggcatcgcgc
    40201 tcttcgcccg tcagctcgct aagttgccca ttgcgcattt ccagcaggcg gtcggcgtgg
    40261 ataaagtaat gatcatcatg actgatagcg aaaatagttt tacccatctc ctgcatcagc
    40321 ggcagcaaca cctgataaaa ctcacgacgg aagtgtggat cctgatccac cgcccattca
    40381 tccagcagga taatatcgcg ttcttctgcc agcgccagca acagcgccac gcgttttttc
    40441 tgcccttttg ataacttcag gttaacaata cgcccgttgc ttaactcaag cttatgagcc
    40501 attttcagct gcgccagcca cttctcaacc agttgcgggt tagcgggttg cccctccggc
    40561 cccagcagtt gatcaaacag ccagacatcg gtaaacactg ccgaaaacag tttgcgataa
    40621 tcttccggtt gttcgccgct gacaggtttg ccatccagca agatttcgcc gctttgtggc
    40681 tgatacaagc ccgtcaacaa catcgccagc gtcgattttc cgctaccgtt accgccaatc
    40741 agaaacagca gctcgccacg tttgatggtg agattaatcg gaccaacgga aaacgcgtta
    40801 tcctgataag caaacgtcac gttacgcagc tccagcgttt gccagttggg aaacgcctgc
    40861 gggcgcggaa actcagcttt gaaaggcgcg agcgcgaatt tgttcagctt gttaaacgcc
    40921 acctgcgccg tcagcagcgt cggcaatgcg ccaaccgccg aaagcagcgg cgtacgcagg
    40981 aataacagcg tcaacgaata ggtcgcagca acgttggtat cagcccaacc gagactgttc
    41041 gccatccaga acaccaggcc gatagcgccc agcatcatga tgtttgacca gttcacggca
    41101 ctaagatgga aggtgtctgc gcgaataata tgatggcgat actcttgcgc atcggggatg
    41161 tagaggttgt taaacacata ctcggcgcgt tcccggttca gagtcagctc tttgcgccct
    41221 tcaagtacag tttgaaaatc cgtgtacagc ttgtcttcgg tttcacgtag ggtcgccata
    41281 tgtttgtaca cccgcgccac cagcacaaaa ccgccccaga tggtgatcgc catccagata
    41341 gccgttacca gcaacatttt gcccgacagc atccacagat acgccgatga accgatagtg
    41401 agaatgatcc cctgccccag ttccggcaga cgcacaaaag caatggtgat attgcgcaca
    41461 tcgctggtta atcccgccag caacgaggcg ctaccgagtt gttcaatgcg ctcgacgtgg
    41521 gtatccagaa tccgcttgat aaattcacta cgcagtcggt aaacgaagtg atgccccaaa
    41581 gtggtgagcg ccagttgcga tccgagagtg actgccatca acagcagcaa taatcccaga
    41641 aactccggta acaccagcag actggtatcc gccgtttcga taaggcgctg attgataaaa
    41701 gcaattaagc caatgcctaa tgccgcactg gcgaggctta gcgccatcac actgataaat
    41761 ggccagcgat actgccgcca gacaagtaca agaagttcca tgcagaaaac ccggacaatg
    41821 aattacagcc cgcagtttaa acatcttcgc gcgcacagca ataataattc ttatttttat
    41881 tcttttttac ctgcctgacg gaatgtcagg ttgtagcggc agtcggtggt gagtggatga
    41941 aaccccgctt tcaacggttg aataccgtga taaaacagcc gcgattcacc gccccatacc
    42001 accacatcgc catgttccaa caacaaacgt ttgagcggat cattacgttt caggccacca
    42061 aattgaaaaa tcgcgggtaa gcccagagaa acagaaacaa ttggcgcgcg cagatccggt
    42121 tcgtctttat cctgatgcag cgacagtttc gcgccaggag cgtagcggtt gataagacaa
    42181 gcatctggct ggaaatctgg atagcccgcc gccgtagccg cacgttgaca taaatcatga
    42241 aagctttgtg gcatggcggg ccacggttta tttgtttgcg gatcaatggg cgaatagaga
    42301 taaccttgcc gatgggtcgt ccagcccaga tgcccacagt tggtcatcgc caccgacatg
    42361 gtatatccgc ctggagcgac catctggcga aacggcgact ggctggcaac gtcattaata
    42421 tcgcggatta gttgctccgc agcattaaaa gcaaaacgcc gtaaaattac cgcaccagcc
    42481 gccagtggct cttgccacgg ttcagcatcg gcaaacagat ccaacattac ctctcctcat
    42541 tttcagcttc gcggcgcagc agttgcgctt tacgcgacac gccccagcgg taaccggaaa
    42601 gtgtgccatc accacggacc actcgatgac agggtattac gatagccagc ttgttggcgg
    42661 cacaggcgct ggcaacggcc cgtaccgctt tcggtttgcc gatggcgtta gccagttgct
    42721 gataactgac ggtttcacca caaggtatcg tgcgcagtgc ctgccagact tgttgctgaa
    42781 aagcagtgcc gcgaatatcc agcggtaacg tcagcggcgt atcgcgttga ttgaggctgg
    42841 cgatcacttc acgcacatgt tgctgaaacg tcagatcggc aggcgcgttg tcggcagcgg
    42901 gaaacatctg ctgcaactcg ctgattaatg tcgcgtcatc atcgcccagc aatatcgcgc
    42961 aaatcccccg ctcgctttct gccaccaggc aacgacccag ctcacaatca gccagcgcgt
    43021 aacgcaccgc cagattttcg ccaccgtgac ggaattgttt agccgtcatg cccagcgttt
    43081 catcggcttt gcggtagtaa ctgctgctgt cggggaatcc ggcgttaaga atagacgtcg
    43141 tcacgctctc ccctttcgcc agcgattcgc gcaaacggcg agcgcgccag gcctgttgcc
    43201 aggctttagg cgtcattccg gtagtagctt taaacaaccg atgtagatga aatggactca
    43261 tcgccacctg gtcggctaag gcttccagcg ttacaggcgt ttcctgttcc agcagtcgac
    43321 acgcgtgggt gattttatcc aaccgatgtt gccggggatt ggctttgtct ggctgacaac
    43381 gtttgcaggg gcgaaatccg gcggcgagtg cctcgctggc atttgcgtag aaggagacat
    43441 tttcccgcaa ggcatgtctg gcgcggcaag aaggacggca aaagataccc gtggtgcgca
    43501 cggcgaaaac gaattcgccg tcggcattcg ggtcgcgggc taagacagat tgccagcgtt
    43561 gatcgtcagt taagcatgtg gcgtttttca taatcagctc cctggttaag gatagccttt
    43621 agtctgcccg gtcaccatca cgcaaaaacc aacaatcttg cgctttaatt tttttcgctg
    43681 acaaggaagc ttttaaactg tggtgacatc caggttttaa agctatcgcc ttctttggtg
    43741 atcatataaa ccgccagccc ttcccggcga acaacctctt tggctttctc cggcccgagt
    43801 accatcaagc cagtatccca ggcatcggct tccagcgccg tcggagcaat caccgtcacg
    43861 gataccagat tgtgttcgat gggacgcccg gtttgcggat cgataacatg ggaaagacgt
    43921 ttgccgtcca gttcgtaata gttacggtag ctgccagagg tgctaatccc atgaccgttg
    43981 atatccacca cagcctgaac cgcgttttct ttatcggttg gtttttgaat cgctacccgc
    44041 cacggctggc cttcaccgtt cataccacgg ctgttcagcg cgccgcccac cgacaccaga
    44101 tagcgggaaa tcccttcctg ctccatcaag cgtgccaggt gatccgccgc ataaccttca
    44161 ccgacggtgg agagatcgac atataaatcc ggcaggtctt tttgcagata ttgctgatgc
    44221 gactgattga tgaccgtcag gtgctgtaag ccggttttgg ctttcatcgc atcgatctgt
    44281 tcctggctcg gaatttgaac cggctgttgt tccgggccaa agccccacag attcaccagc
    44341 ggcccgacgg taatatccat cgcaccatcg gtccttgccc caatgcgcag cgaggtggtg
    44401 acgatatcgg ccatcgcttc acttaccggc cacggcgaca aactttgcga gtcgttaaag
    44461 cgcatcagcg cggaatcttt tttataggtc gaaagcagct gatcgtcggc gtccagctgg
    44521 gtctgaatct tttctttaag ttcggcactg cgtttggcat caatgcccgg gatgctggca
    44581 cgccagaagg tacccatggt tttgccttca agaacggtaa cttcagtagc gtgggttttg
    44641 gcgggttgtg gtttttgatc gcaaccaaca aagaagagcg cggcaaccag cagtgccacg
    44701 cgggtaaagc ttatttccat acgtgattat cctcatgcga acggtcgcaa gagtacacca
    44761 aaaaactgtg tttgtacgct gaaaacaatg aaaaaagggc ccgcaggccc tttgttcgat
    44821 atccatcgag attagaactg gtaaaccaga cccagagcta cgatgttatc agtgttgatg
    44881 ccagcgtcac gagtgaactg gttgtcgtcc agcaggttga ttttgtagtc aacgtaggtg
    44941 gacatgtttt tgttgaagta gtaggtcgca ccaacatcaa catatttcag gatatcttcg
    45001 tcgtcgtagt tacgaccatt gatgacaccc aggtttttac ctttagactg caggtaagcc
    45061 agggacggac gcagaccgaa gtcgaactgg tactgagcaa cagcttcgaa gttctgtgct
    45121 ttgttcgccc aacccaagtt gcctacgcga gttgcgttgt aggtctgggt gtactgagca
    45181 gctaggtaga tgttgttcgc gtcgtatttc agaccaccgg tgtaggtttc agcgcggtcg
    45241 ccattaccga tgtaacgctc atcataacgg ttcagaccga agttctggtc atcagtacgt
    45301 ttggagctgg aaactgcagc accgataccg aagccttcgt aatcataagt aatagaaccg
    45361 ccaacgccgt caccgttctg acgcagagct tcacgaccgt tgttggtcat gccttcacct
    45421 tccgggctac cgtttttgcc ctgatactga acagcgaagt tcagaccgtc aaccagacca
    45481 aagaagtcgg tgctacggta ggttgcgaag ccgttaccac gctgctgcat gaagttgtca
    45541 gaaccgtaag tgtcaccacc gaattctggc agtacgtcgg tccaggaagt tacgtcgtaa
    45601 actacgccgt agttacgacc gtagtcgaaa gaacctacat cctggaattt cagacctgcg
    45661 aatgccacac gggtccagga gttgttttcg ttttcagcgc tgttgccctg gatctgatat
    45721 tcccactggc cgtaaccggt cagctggtca gtaacctgag tttcaccttt gaagccaaga
    45781 cgcatgtagg tctggtcgcc atctacagac ttgtcgtcag agaaatagtg caggccgtct
    45841 actttaccgt acagatctaa tttgttgccg tctttgttgt aaacttcagc agcgtttgct
    45901 gcgcctgcta ccagcagagc tgggaccagg agggacagta ctttaacttt catgttatta
    45961 accctctgtt atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat
    46021 taatcagtcg gcaagtccat tctccccaaa aatgcagaat aatccaacac gaatatgata
    46081 ctaaaacttt taagatgttt catttatcgc tatagatgtt tcaaaatgta aatacaaggg
    46141 aactttttaa gattattgcg gaatggcgaa ataagcacct aacatcaagc aataataatt
    46201 caaggttaaa atcaataact tattcttaag tatttgacag cactgaatgt caaaacaaaa
    46261 ccttcactcg caactagaat agctcccgct atcatcatta actttattta ttaccgtcat
    46321 tcatttctga atgtctgttt acccctattt caaccggatg cctcgcattc ggttttttta
    46381 cccttcttta cacacttttc attattctgt gctaccacag aaaaactata acgcttgtta
    46441 actatttcac aaataattaa catcagcata atttccagca atctttgttt atttgcaatt
    46501 aatttcgttg ggctttttgt aggttatttg tacagcaaaa tggcgcttgt acatctattt
    46561 ccccccatac aggatgataa atatcacggg agaatagaga atcatcaatc aggtaagagt
    46621 ctggaatttc acactgtacc ctttatactg ccctatcact tcgcgaagtt ttaacaggtc
    46681 ataaacacga atgcgtcaga aagagacaac ggccacgacc cgcttttcac tcctaccggg
    46741 gagcattacc cgcttctttt tactgttgat cattgtgtta ctggtgacga tgggggtaat
    46801 ggtacaaagt gccgttaacg cttggctgaa agataaaagt taccagattg tcgacattac
    46861 ccacgcaatc caaaagcgcg tcgatacctg gcgttacgtg accaggcaga tctacgacaa
    46921 cattgccgcg acgacctccc cctcctccgg cgaaggttta caagagacgc gcctgaaaca
    46981 ggatgtctac tatctggaga aaccacgccg caaaacggaa gcgttaatct ttggctctca
    47041 cgacaactca acgcttgaga tgactcagcg gatgtccact tatctggata cattgtgggg
    47101 tgcagaaaat gtaccgtggt cgatgtatta cctgaatggt caggataaca gtctggtgct
    47161 gatctcaacc ctacccctta aagatctcac ctccggattt aaagaatcga ccgtcagtga
    47221 cattgttgat tcacgtcgtg cagaaatgtt acaacaggcc aacgccctcg atgaacgcga
    47281 aagtttttct aacatgcgcc gcctagcctg gcagaacggt cattacttta ccttgcgtac
    47341 cacgttcaac cagccgggac atctggcaac ggtcgtggct tttgatctgc cgattaatga
    47401 tttgatccca ccgggtatgc cgctggacag tttccgcctt gagccagacg cgacggcaac
    47461 gggaaacaat gataatgaga aagaagggac ggatagcgtc agtatccact ttaacagtac
    47521 gaagattgaa atctcctcgg cactcaactc taccgatatg cgcctggtct ggcaggttcc
    47581 ttatggcacc ttattgctgg atacgttgca aaacattctg ctgccactgc tgctgaacat
    47641 cggtttgctg gcgctggcgt tatttggcta taccacattc cgccatttct ccagccgcag
    47701 tacagaaagc gtccccagca cggcggtcaa taacgaattg cgcattttac gggcaatcaa
    47761 tgaagagata gtctcactgt tgccgctcgg cctgctggtt cacgatcagg aatcgaaccg
    47821 cactgtcata agtaacaaaa ttgccgatca tttgctgccg catctgaatc tgcaaaacat
    47881 caccaccatg gcggaacagc atcaggggat tattcaggcg acgatcaata acgagctgta
    47941 tgagatccgc atgttccgca gccaggtcgc gccgcgcaca caaattttca ttattcgcga
    48001 tcaggatcgc gaagtgctgg taaacaagaa actcaagcag gcgcagcgtc tgtatgagaa
    48061 aaaccagcag gggcggatga cctttatgaa aaacattggc gatgcgctga aagaacccgc
    48121 acagtccctg gcggagagtg cggctaaact caacgccccg gaaagcaaac aactggcgaa
    48181 tcaggcagat gtgctggtgc ggctggtcga agaaatacag ttagcgaaca tgcttgcgga
    48241 tgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag gatttaattg atgaagttgt
    48301 gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa ctgctgatta acaatcatct
    48361 gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta cgacgtattt tgctgctact
    48421 gatgcaatat gccgtgacct caacgcaatt gggaaaaatc acccttgagg ttgatcagga
    48481 tgagtcctct gaagaccgcc tgacattccg cattctggac acgggagaag gcgtaagtat
    48541 tcatgaaatg gataatttgc acttcccgtt tatcaaccag acccaaaacg atcgttatgg
    48601 aaaggcggac ccgctggcat tctggctgag cgatcaactg gcacgtaaac tgggcggtca
    48661 tttaaacatc aaaacgcggg atgggcttgg tacacgctac tctgtgcata tcaaaatgct
    48721 cgccgctgac ccggaagttg aagaggaaga agagcgttta ctggatgatg tctgcgtaat
    48781 ggtggatgtt acttcggcag aaattcggaa tattgtcact cgcctgttag aaaattgggg
    48841 tgcaacctgt atcacacccg atgaaagatt aattagtcaa gattatgata tctttttaac
    48901 ggataatccg tctaatctta ctgcctctgg cttgctttta agcgatgatg agtctggcgt
    48961 acgagaaatt gggcctggtc aattgtgcgt caacttcaat atgagcaacg ctatgcagga
    49021 agcggtctta caattaattg aagtgcaact ggcacaggaa gaggtgacag aatcgcctct
    49081 gggcggagat gaaaatgcgc aactccatgc cagcggctat tatgcgctct ttgtagacac
    49141 agtaccggat gatgttaaga ggctgtatac tgaagcagca accagtgact ttgctgcgtt
    49201 agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg ctaaatctgg tacccggcaa
    49261 gcagttatgt gaaacgctgg aacatctgat tcgtgagaag gatgttccag gaatagaaaa
    49321 atacatcagc gacattgaca gttatgtcaa gagcttgctg tagcaaggta gcctattaca
    49381 tgaacaatat gaacgtaatt attgccgatg accatccgat agtcttgttc ggtattcgca
    49441 aatcacttga gcaaattgag tgggtgaatg ttgtcggcga atttgaagac tctacagcac
    49501 tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat taccgatctc tccatgcctg
    49561 gcgataagta cggcgatggc attaccttaa tcaagtacat caagcgccat ttcccaagcc
    49621 tgtcgatcat tgttctgact atgaacaaca acccggcgat tcttagtgcg gtattggatc
    49681 tggatatcga agggatcgtg ctgaaacaag gtgcaccgac cgatctgccg aaagctctcg
    49741 ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt ttctcgcctg ttggaaaaaa
    49801 tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa agagagtgaa gttctgcgcc
    49861 tgtttgcgga aggcttccta gtgaccgaga tcgctaaaaa gctgaaccgc agtattaaaa
    49921 ccatcagtag ccagaagaaa tctgcgatga tgaagctggg cgtcgagaac gatatcgccc
    49981 tgctgaatta tctctcttct gtgaccttaa gtccggcaga taaagactaa tcatctgtag
    50041 gccagataag acgcgttagt gtcttatctg gcatttgcac cgattgccgg atgcggcgta
    50101 aacgccttat ccggcctacg attcccatta tttcaacaga ttacattaaa gtaggccaga
    50161 taagacgcgt cagtgtctta tctggcattt gcactgaatg ccggatgcgg cgtaaacgcc
    50221 ttatccgtcc tacgaatccc gcgatttcct gaccctctcg gcatataccg tcagcgtctg
    50281 ttttatcaca tccagcgtta ccggcttcga gaggcagctg tccataccgg actccagaca
    50341 ccgctgcttc tcttcagcca acgcattagc agttactccg attaccggca acgtcagtcc
    50401 caactgacga atgcgttgcg tcaagcggta accatccata tttggcatgt tgacgtcgct
    50461 aagcacgata tcaatatgat tcttactaag tacattaagc gcatcgacgc catcattcgc
    50521 ggttttacat tgatagccca acgatcccaa ctgatctgcc agcagacgtc ggttaatcgg
    50581 atgatcatcc acgaccagaa tcatcatatc gtcgttatcg ctgaccgctt tgtccgtcga
    50641 cggcagagcg ttagcaggat cgtcgctctc catctcgatc aaataaatac gcgccaacaa
    50701 tgccggtagc tcatgcggag cagccacact gtgtacccac tcccctggcg ctttctccag
    50761 cggaatacca atatggcgac gacagaaggt cactaccgct ctgccctgcc attttttact
    50821 cactacctcg tcagtgatta acacatcttc gggagtcggt tcctgccctt cgtatgttgt
    50881 aacgacgatg ccgctgcgct gcaaactggt ttccaggaac tgacagagcg acgcattgcg
    50941 gaccgccagc cagcagcgtt taccactcaa cccttccacg ccttttttct gcgggtactg
    51001 agcgccgtac aacggaatac gcacggtaaa ctggctgccc attcccggtt ctgaatctac
    51061 cgagatatcg ccgtccatca tgctgatcag tttttcacaa atcgccagac ccagaccggt
    51121 cccctggaag tttcgctgta cgcccgttcc aacctggaag aagggatcaa acaagcgcac
    51181 cacttctttc gccggaatcc ccacgccggt atcgcgaaca cggatagaga gataatcgcc
    51241 atcagcgcga acatgcaaaa ctatacagcc ggtatcggtg aattttatgg cgttactcaa
    51301 caggttggag atgacctgct gtaaacgcat cgggtcgcca tttaaggcca ctggcacatc
    51361 cggttcaata aagcagtaca agcctaactg cttgcgtacc accagcggta aatagttggc
    51421 ggtgatgtgg ttcatcactt cacgcggtga aaactcacgc ggttcgatct tcaactgttc
    51481 cgattcaatc ttcgagaaat cgagaatatc gctgataatt ttcaacaaca ggctggaaga
    51541 gttgttcatt accgtcacca gacgatcgac gcctttcggt aactctttgg tttgcaacag
    51601 atccaggtta ccgataatgc catacagcgg cgttcgcagc tcatgactga cggtggcaag
    51661 gaacatcgat tttgactggc tcgcctgttc cgctgcttgt gccatctcct gcaacgactc
    51721 ttccatcttc acgcgcgaag aaacatccac cagcacacaa atggccacgt tttcattacg
    51781 atagcgcgaa tggacgaagc tgatttgcag attggtattg ttgctggtca ggacatcaac
    51841 aaaattgacc tgctgcccac agataatttg cgtcagtcgt tggcggtcct catgcgtaag
    51901 catattgaga taggtatgcg ccagttcgtt acttaaaata ttgacgccat cagcggtacg
    51961 caaaatgcag atacccactg gcgcggaggc gacaatcttg cgattgaact gctcatgttc
    52021 ttccagtcgc agggcgtcgc tttccgccgg aatgaaaata cgtcgctcgt acatccgcgc
    52081 gagagtaaac aatgcagctc cggcaagcac attcagcaaa attgcgttaa ggatcaacat
    52141 gcgaatgcgt tccagcacct tatcaaccgg caccgaatac acgatgctta gcgatgaggg
    52201 tggcagattt ttcttcagca ccagctcccg gaacccttcc gtatagccaa accaggagcg
    52261 ttcctgcatc cagcgaggat cgcccttaat tttactttct ggtccggtaa gcgaaatcag
    52321 ggtatgacca ttttcatcaa gaatggtaac ccccatcggc aacgtacccg gtaagaaaaa
    52381 gttctccatc cggatggtct gctcgacacc caaaagcgcc tgcaaccggt tcgccagata
    52441 aactggcgtc aacgcgtaaa aatacccgac gcaagggcgc ggaccttcgc tgatccagta
    52501 gaggttactg ccgctatcat cttgtggtgc atttcgatat ttattgatgc gttcatgcaa
    52561 cgctttcaac gcggtatcgc gttccactgg catatcacgc agaccgaaat tggccatgca
    52621 gaggttatca ctgccgatta aaaatacccg gttgagatcg taagccgcag aaaaattatc
    52681 gcgccagtag cgcataaacc acgccaatga ctccagagaa cctcgccagg tgttactcat
    52741 tgcggaacaa tcggagtcgg caaacagcgg ttcaaacgca ggcacatccg cctgcgtttc
    52801 tcgtccacgc ggggaaagca caccgttttc tgccgataag cgattttcgg cgatgtactt
    52861 aagctctttc atcacatcag aggtgcgttg aataaagcgc tgagcctgat cggaactcag
    52921 attaaattcc tgacgaattt ccgattctcg ctgatgtaac gcattaacga tgtaaaaaac
    52981 ggatgaaaaa gcaatcaaca gccagagcac taacgccaat gctctgaaca tgtagcgcga
    53041 ggctttcagg gttgtacgaa aagaagcaag gtatttcaaa ggggagaagc tccgcctcag
    53101 gtgaccgatg gagtgtggtt aaggtagcgg taaaagcgtg ttaccgcaat gttctgtcac
    53161 cattgcaata atgcgtatcg cttagaaacc attgccttaa ccaggaaagc cggagtagat
    53221 ggacagttct cagtacgaaa aataagaaat gaccaatctg ccgacgcttg ttaatggtta
    53281 tatcctggcg tcggcagatc gcccagcagt cttatctgaa aaaagagaga gccaggttgt
    53341 gtgaagggat ggatcaccag tcaaaacttt tcaccagcgt cagctcgcca gcattacgca
    53401 tcggtacaat aaatgtttcc tgtttctcat tgaccgatcc ttcatcggtg atcagcgtca
    53461 gttgggcggt cgtcaattcc gtttcgctgc gcccaccata gtagttgata tacacctgat
    53521 agcgcccgtg aactggtgct ggcatggcga aaatctcggg tccgtacccc gtcgtgacat
    53581 ccatatccag tgcaccactg tttttcagca cggtgttacc gtaccaggcg tgttcgccat
    53641 cgggcgtgac aacatggaga tcgagatcgg tattgtccgt atcccacgag agaaccagcc
    53701 gtaaacgtgc acgaatagtt cctgtacccg gcgttgagta aaactgcatt ttttgccggc
    53761 tttgcccatc cgggctgatg acctgcacgc tgttgctgcc ttcagtgaaa atataagggc
    53821 gtgcaaatga accatcggat tcaattctct gcggcatact ggcaccgttg accaccagcc
    53881 ggccctgctg aactttaccc gccgcagcat aattctttat tttgccgcgg atctgcgctg
    53941 agatattttg atcatctgcc atgttgacgg acgacgccgg gtaattgatc gattgtgaaa
    54001 aactggcatc ttcgccctct gccggatgcc agccagagag cggtgcgtca atctcgactt
    54061 cctgcacacc ttcactgtga gcgacaggcg aaagtgccac cagtaacaac ggaagaaaaa
    54121 tctttcgcat agcattagtc cattaataat tgtcgggtca gcgtttcgat atagttctca
    54181 tcaagcccgg tgggataacc atcaaaggcc aagtgcagat actcatgggt taaatccagc
    54241 cgatcctgaa gcgtaaagaa gttacgaata aacagccgtt tctgctgccg atcggtatag
    54301 gggaaacctg agactaaacg acagaccgca aacacgtctg gttcgttgta ccccgtctcg
    54361 ccttgtaata tacgccgcca ctgcggcatt tttttcgcca gccaggcttt cgctttgggt
    54421 aataactggc aggttgagcg tggagctccc cagcggctaa ggctgttgtc gggataagca
    54481 aacgcgagta tctggtcgta acgctccccc tgccccgcct gggccgtggc ttgccgccag
    54541 gaaagtgtcc cttcggtagc gcgactgcca tgataatgaa ccggatcgcc tgcatagatg
    54601 agctcctgcg tccaggcggt catcgttcgc gcccccgtgg tcgctggcga agcagaaaca
    54661 cgctgcgtgg cgctgctgtc ggggatggtc aggcaatcac cttcccgatt ggcgttttgc
    54721 tgtaaaaaag tacgtatcgc cacagtcatg gctttcgctg cctcaggtgg cgtacttttc
    54781 gcctcgcgat ccagaactct ggtaacgtac tcttcacgat cgagatgcga ctgcaatttc
    54841 agtttgccct tttcgctcag taaggtcgtc tcaccgtggc tgacaaacgt aatgtggtta
    54901 ccattagtaa acgtcacacg atagcgccca ttaagtacgc tgggttttac tgccgtcgta
    54961 ctcttttccg ctgtgatttt cttcagcgga tagcgggcaa acagctccac ctctacacat
    55021 tgcccactgg caacctgcgt cggcactggc aaaacccgat taagcaccgt cgcataacgg
    55081 gttagtaccg ttttgctggt gccactgcca gtaacccaca gcggtgtacc atcggttaac
    55141 cagccagcaa aaccaccctg acggatttcc tgtttatcat cggcaaacca gctccaggtt
    55201 ttcacccgta cccgactacc gagcatggat gcgacaccga ttttcgcctc gtccagcacc
    55261 acatccagta agacttcctg agctttgttt tgcgcaggta acgtcgctaa tgaatcaagc
    55321 agcgatttaa ccgttaccga ggtttctggc ttcagcgttg taagagacgc taaccacgca
    55381 ggtgcctgac gctgttgcca gtattgcccc cacacatctg cgccaatatg caaccgctgc
    55441 ggcgcaaaat agagcccaca ggaacgtacc agcgcggtat cgcgcgttat gctctcccca
    55501 gcctgacaac aataaacctc ttccggggag tttccgcgac actggtaggg ctgctccggt
    55561 tgatgggtat cctccagcca ggcgtaaaca tacagtttcc acaaactccc taacggcgtg
    55621 gtgagcgagt caggtaatgc tgagactttg gtcacgcctg atgacgacag ctgatacagt
    55681 tgatcgtgct gcgcaccgcg cagcaccagt tgtaacggcg tctcctccgc aagcgttggc
    55741 agagtaacca acgccaatag ccagacgatt ctgcgccagt tcacttattt cacctgcatc
    55801 ccggtccatt cactccctgc cgcgacactt tgctgcgcag gtgcatagga acgcacataa
    55861 cgagcaggag gcagaacgaa ttgccctttt tgcgagaagc gcagcaaatg gcggaaagtg
    55921 accgttcccg tcagttcttt caccggcacc atataggcca gttcgcccat ttcattacgc
    55981 gctttttcca gcaattgccc ctgctgtttc gcggcgttgg gtttattgac tgaaatgccc
    56041 catgttgtgc gctcaacgtc ggctcccggc gggagcggta cttccacctg accatagcgc
    56101 agaactgcat cctgctcgct ggtaagcgta atttcatcga gatacagcgc atcgctgtca
    56161 atctcattgc tggtcaccgg ctgcagagta aagctcatct cttcttcacc ggggataagc
    56221 cgatacaact ggcgttcaac ggtcaccgga atgttacttt gttgagccgt ttttgccggt
    56281 tcacgccagc ggacctgcac atttgcggcg ataattcgtc accaaaagag agaatgtccg
    56341 gcacacccta accaacccaa cgccagtctt cgccccctcc agttaactta tgtttagccc
    56401 atgcgcccgc aggcgcaggc aaaacaactg gaggcattgt cgccatatat ttcgccagcc
    56461 agttcatggc cagcgcgcgc tcaatagtgg attgctcagc ggtcaaacca ttaaaatagc
    56521 ggccgtctgg gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg
    56581 attgctgttt gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt
    56641 caactgcgct tttttcagca acgtcatggt taagacgttt gccaccgcat ctgccagtgg
    56701 agattccggt gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa
    56761 atcttcatct ttagttccgc gccgggcgat agcttcatcc ccccctttca acaacgtttt
    56821 gcacggcaga ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat
    56881 gttatccgcc tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt
    56941 tacgccgagc gcctggctgg cctgccagtc ggcgtaccat gcccatgccg taaggaaggc
    57001 gtcaccattg ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat
    57061 cagccgcaga cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg
    57121 atcggcaagc gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac
    57181 cccaccccac ggttcatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt
    57241 ttcactactt tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc
    57301 accgccgagc atgacatttt tctgctgttc aacgggccag ctgttgtcca caaaagacag
    57361 ttttgtgcta atgctgtcct gcacttgccc attttgttgc agttctgcac ttaacaagcc
    57421 agattgctga atattctgcg tcagcgaaat ataattcgcc cctttgtgca gcgtcagcgt
    57481 ctggcgcatc tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg
    57541 actgaagata aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact
    57601 ccacttcatg tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc
    57661 gtcgccgttc atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa
    57721 cgtgaaatac gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc
    57781 cacctcttca cgccgtggac gttcaggcat ttttactcgc cgctcgctgc ggttagtcgc
    57841 gccaggcgca accggctcgc tagagagcgc ctggtcatag ctgataaacg acagactgga
    57901 gctggtacgc acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat
    57961 ttctggttgc agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac
    58021 aggtttacct ttcagcgacg aggttaat
//
LOCUS       DAJVRP010000013        55430 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVRP010000013 DAJVRP010000000
VERSION     DAJVRP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.013"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OQN12_000776"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OQN12_000776"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1653226.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OQN12_000777"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OQN12_000777"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1653227.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OQN12_000778"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OQN12_000778"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1653228.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OQN12_000779"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OQN12_000779"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1653229.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OQN12_000780"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OQN12_000780"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1653230.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OQN12_000781"
     CDS             7826..9559
                     /locus_tag="OQN12_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1653231.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OQN12_000782"
     CDS             9708..10559
                     /locus_tag="OQN12_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1653232.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OQN12_000783"
     CDS             complement(10546..10887)
                     /locus_tag="OQN12_000783"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1653233.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OQN12_000784"
     CDS             complement(10889..11767)
                     /locus_tag="OQN12_000784"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1653234.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OQN12_000785"
     CDS             complement(11733..14030)
                     /locus_tag="OQN12_000785"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1653235.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OQN12_000786"
     CDS             complement(14081..14401)
                     /locus_tag="OQN12_000786"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1653236.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OQN12_000787"
     CDS             complement(14416..15495)
                     /locus_tag="OQN12_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1653237.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OQN12_000788"
     CDS             15804..16907
                     /locus_tag="OQN12_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS1653238.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OQN12_000789"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OQN12_000789"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1653239.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OQN12_000790"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OQN12_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OQN12_000791"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OQN12_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="OQN12_000792"
     CDS             20366..20983
                     /locus_tag="OQN12_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS1653240.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="OQN12_000793"
     CDS             complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="OQN12_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1653241.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="OQN12_000794"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="OQN12_000794"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1653242.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="OQN12_000795"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="OQN12_000795"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1653243.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /gene="metL"
                     /locus_tag="OQN12_000796"
     CDS             complement(25757..28189)
                     /gene="metL"
                     /locus_tag="OQN12_000796"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1653244.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="OQN12_000797"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="OQN12_000797"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1653245.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="OQN12_000798"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="OQN12_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1653246.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="OQN12_000799"
     CDS             30130..30738
                     /locus_tag="OQN12_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1653247.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="OQN12_000800"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="OQN12_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1653248.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="OQN12_000801"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="OQN12_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1653249.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="OQN12_000802"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="OQN12_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1653250.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="OQN12_000803"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="OQN12_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1653251.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="OQN12_000804"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="OQN12_000804"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1653252.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="OQN12_000805"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="OQN12_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1653253.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="OQN12_000806"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="OQN12_000806"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1653254.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="OQN12_000807"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="OQN12_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1653255.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="OQN12_000808"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="OQN12_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1653256.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40780
                     /gene="glpF"
                     /locus_tag="OQN12_000809"
                     /pseudo
     CDS             39935..40780
                     /gene="glpF"
                     /locus_tag="OQN12_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40820..42310
                     /gene="glpK"
                     /locus_tag="OQN12_000810"
     CDS             40820..42310
                     /gene="glpK"
                     /locus_tag="OQN12_000810"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1653257.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42540..43550
                     /gene="glpX"
                     /locus_tag="OQN12_000811"
     CDS             42540..43550
                     /gene="glpX"
                     /locus_tag="OQN12_000811"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1653258.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43647..44393
                     /gene="fpr"
                     /locus_tag="OQN12_000812"
     CDS             43647..44393
                     /gene="fpr"
                     /locus_tag="OQN12_000812"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1653259.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="OQN12_000813"
     CDS             complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="OQN12_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1653260.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44853..45152)
                     /locus_tag="OQN12_000814"
     CDS             complement(44853..45152)
                     /locus_tag="OQN12_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1653261.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45364..45804)
                     /locus_tag="OQN12_000815"
                     /pseudo
     CDS             complement(45364..45804)
                     /locus_tag="OQN12_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45905..46504
                     /locus_tag="OQN12_000816"
     CDS             45905..46504
                     /locus_tag="OQN12_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1653262.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46612..47379
                     /gene="tpiA"
                     /locus_tag="OQN12_000817"
     CDS             46612..47379
                     /gene="tpiA"
                     /locus_tag="OQN12_000817"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1653263.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="OQN12_000818"
     CDS             complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="OQN12_000818"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1653264.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="OQN12_000819"
     CDS             complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="OQN12_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1653265.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="OQN12_000820"
     CDS             complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="OQN12_000820"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1653266.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="OQN12_000821"
     CDS             complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="OQN12_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1653267.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="OQN12_000822"
     CDS             complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="OQN12_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1653268.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52448..53146
                     /gene="cpxR"
                     /locus_tag="OQN12_000823"
     CDS             52448..53146
                     /gene="cpxR"
                     /locus_tag="OQN12_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1653269.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53143..54516
                     /gene="cpxA"
                     /locus_tag="OQN12_000824"
     CDS             53143..54516
                     /gene="cpxA"
                     /locus_tag="OQN12_000824"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1653270.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="OQN12_000825"
     CDS             complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="OQN12_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1653271.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13506 a        15021 c        14159 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggt gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagacca tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacagccg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcatgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccatacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta
    40261 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    40321 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    40381 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    40441 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    40501 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    40561 tccgaaagtc tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    40621 cattccttac ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt
    40681 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    40741 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    40801 acatgactga aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    40861 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    40921 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    40981 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    41041 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    41101 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    41161 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    41221 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    41281 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    41341 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    41401 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    41461 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    41521 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    41581 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    41641 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    41701 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    41761 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    41821 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    41881 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    41941 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    42001 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    42061 gcagttccag tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
    42121 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
    42181 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
    42241 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
    42301 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
    42361 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
    42421 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
    42481 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    42541 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    42601 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    42661 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    42721 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    42781 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    42841 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    42901 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
    42961 ataacctgcg caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
    43021 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
    43081 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    43141 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
    43201 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    43261 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    43321 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
    43381 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
    43441 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
    43501 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat
    43561 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    43621 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    43681 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    43741 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    43801 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    43861 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
    43921 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
    43981 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    44041 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    44101 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    44161 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    44221 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    44281 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    44341 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    44401 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    44461 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
    44521 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    44581 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    44641 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    44701 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    44761 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    44821 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    44881 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    44941 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    45001 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    45061 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    45121 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    45181 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    45241 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    45301 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    45361 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    45421 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    45481 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
    45541 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    45601 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
    45661 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
    45721 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    45781 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    45841 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    45901 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    45961 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    46021 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
    46081 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    46141 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    46201 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    46261 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    46321 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    46381 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    46441 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    46501 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    46561 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    46621 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    46681 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    46741 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    46801 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    46861 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    46921 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
    46981 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
    47041 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    47101 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    47161 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    47221 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    47281 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    47341 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    47401 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
    47461 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
    47521 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    47581 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
    47641 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    47701 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
    47761 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    47821 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    47881 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    47941 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    48001 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    48061 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    48121 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    48181 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    48241 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
    48301 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    48361 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    48421 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    48481 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    48541 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
    48601 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    48661 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    48721 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    48781 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    48841 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    48901 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    48961 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    49021 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    49081 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    49141 gtttccagtg ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    49201 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    49261 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    49321 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    49381 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    49441 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    49501 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    49561 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    49621 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    49681 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    49741 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    49801 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    49861 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    49921 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    49981 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
    50041 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    50101 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    50161 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    50221 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    50281 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    50341 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
    50401 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
    50461 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    50521 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
    50581 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    50641 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    50701 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    50761 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
    50821 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    50881 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    50941 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    51001 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    51061 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    51121 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    51181 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    51241 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    51301 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    51361 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    51421 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    51481 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    51541 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    51601 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    51661 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    51721 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    51781 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    51841 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    51901 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    51961 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    52021 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    52081 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    52141 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    52201 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    52261 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    52321 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    52381 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    52441 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    52501 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    52561 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    52621 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    52681 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    52741 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc
    52801 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    52861 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    52921 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    52981 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    53041 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    53101 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    53161 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    53221 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    53281 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    53341 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    53401 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    53461 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    53521 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    53581 cgtccggcca gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    53641 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    53701 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    53761 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    53821 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    53881 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    53941 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    54001 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    54061 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    54121 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    54181 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    54241 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    54301 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    54361 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    54421 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    54481 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    54541 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    54601 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    54661 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    54721 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    54781 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    54841 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    54901 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    54961 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    55021 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    55081 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    55141 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    55201 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    55261 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    55321 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    55381 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAJVRP010000014        54307 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVRP010000014 DAJVRP010000000
VERSION     DAJVRP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.014"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..758)
                     /gene="glnS"
                     /locus_tag="OQN12_000826"
     CDS             complement(<1..758)
                     /gene="glnS"
                     /locus_tag="OQN12_000826"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1653272.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPV"
     gene            complement(961..2907)
                     /gene="nagE"
                     /locus_tag="OQN12_000827"
     CDS             complement(961..2907)
                     /gene="nagE"
                     /locus_tag="OQN12_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1653273.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLKTEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            3240..4040
                     /gene="nagB"
                     /locus_tag="OQN12_000828"
     CDS             3240..4040
                     /gene="nagB"
                     /locus_tag="OQN12_000828"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1653274.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            4100..5248
                     /gene="nagA"
                     /locus_tag="OQN12_000829"
     CDS             4100..5248
                     /gene="nagA"
                     /locus_tag="OQN12_000829"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1653275.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            5257..6477
                     /gene="nagC"
                     /locus_tag="OQN12_000830"
                     /gene_synonym="nagR"
     CDS             5257..6477
                     /gene="nagC"
                     /locus_tag="OQN12_000830"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1653276.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            6525..7277
                     /gene="nagD"
                     /locus_tag="OQN12_000831"
     CDS             6525..7277
                     /gene="nagD"
                     /locus_tag="OQN12_000831"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCS1653277.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            7674..9338
                     /gene="asnB"
                     /locus_tag="OQN12_000832"
     CDS             7674..9338
                     /gene="asnB"
                     /locus_tag="OQN12_000832"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1653278.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            9717..9793
                     /locus_tag="OQN12_000833"
     tRNA            9717..9793
                     /locus_tag="OQN12_000833"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9751..9753,aa:Met,seq:cat)
     gene            9802..9886
                     /locus_tag="OQN12_000834"
     tRNA            9802..9886
                     /locus_tag="OQN12_000834"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9836..9838,aa:Leu,seq:tag)
     gene            9910..9984
                     /locus_tag="OQN12_000835"
     tRNA            9910..9984
                     /locus_tag="OQN12_000835"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9942..9944,aa:Gln,seq:ttg)
     gene            10019..10093
                     /locus_tag="OQN12_000836"
     tRNA            10019..10093
                     /locus_tag="OQN12_000836"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10051..10053,aa:Gln,seq:ttg)
     gene            10109..10185
                     /locus_tag="OQN12_000837"
     tRNA            10109..10185
                     /locus_tag="OQN12_000837"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10143..10145,aa:Met,seq:cat)
     gene            10234..10308
                     /locus_tag="OQN12_000838"
     tRNA            10234..10308
                     /locus_tag="OQN12_000838"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10266..10268,aa:Gln,seq:ctg)
     gene            10346..10420
                     /locus_tag="OQN12_000839"
     tRNA            10346..10420
                     /locus_tag="OQN12_000839"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10378..10380,aa:Gln,seq:ctg)
     gene            complement(10574..11749)
                     /gene="ubiF"
                     /locus_tag="OQN12_000840"
     CDS             complement(10574..11749)
                     /gene="ubiF"
                     /locus_tag="OQN12_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1653279.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            11895..13319
                     /gene="miaB"
                     /locus_tag="OQN12_000841"
     CDS             11895..13319
                     /gene="miaB"
                     /locus_tag="OQN12_000841"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1653280.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            13472..14512
                     /locus_tag="OQN12_000842"
     CDS             13472..14512
                     /locus_tag="OQN12_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1653281.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            14509..14976
                     /gene="ybeY"
                     /locus_tag="OQN12_000843"
     CDS             14509..14976
                     /gene="ybeY"
                     /locus_tag="OQN12_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1653282.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15066..15944
                     /gene="corC"
                     /locus_tag="OQN12_000844"
     CDS             15066..15944
                     /gene="corC"
                     /locus_tag="OQN12_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1653283.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            15969..17507
                     /gene="lnt"
                     /locus_tag="OQN12_000845"
     CDS             15969..17507
                     /gene="lnt"
                     /locus_tag="OQN12_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1653284.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            17904..18812
                     /gene="gltI"
                     /locus_tag="OQN12_000846"
     CDS             17904..18812
                     /gene="gltI"
                     /locus_tag="OQN12_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS1653285.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            18982..19722
                     /gene="gltJ"
                     /locus_tag="OQN12_000847"
     CDS             18982..19722
                     /gene="gltJ"
                     /locus_tag="OQN12_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS1653286.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            19722..20396
                     /gene="gltK"
                     /locus_tag="OQN12_000848"
     CDS             19722..20396
                     /gene="gltK"
                     /locus_tag="OQN12_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1653287.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            20396..21121
                     /gene="gltL"
                     /locus_tag="OQN12_000849"
     CDS             20396..21121
                     /gene="gltL"
                     /locus_tag="OQN12_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS1653288.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            21239..22174
                     /gene="rihA"
                     /locus_tag="OQN12_000850"
     CDS             21239..22174
                     /gene="rihA"
                     /locus_tag="OQN12_000850"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1653289.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            22258..23919
                     /gene="hscC"
                     /locus_tag="OQN12_000851"
                     /pseudo
     CDS             22258..23919
                     /gene="hscC"
                     /locus_tag="OQN12_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(23979..25430)
                     /gene="djlC"
                     /locus_tag="OQN12_000852"
     CDS             complement(23979..25430)
                     /gene="djlC"
                     /locus_tag="OQN12_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCS1653290.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(25427..26134)
                     /locus_tag="OQN12_000853"
     CDS             complement(25427..26134)
                     /locus_tag="OQN12_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1653291.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            26236..26790
                     /locus_tag="OQN12_000854"
     CDS             26236..26790
                     /locus_tag="OQN12_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1653292.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(26800..28227)
                     /locus_tag="OQN12_000855"
     CDS             complement(26800..28227)
                     /locus_tag="OQN12_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1653293.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLTQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(28224..28931)
                     /locus_tag="OQN12_000856"
     CDS             complement(28224..28931)
                     /locus_tag="OQN12_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1653294.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            29095..30072
                     /locus_tag="OQN12_000857"
     CDS             29095..30072
                     /locus_tag="OQN12_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1653295.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(30142..30624)
                     /locus_tag="OQN12_000858"
     CDS             complement(30142..30624)
                     /locus_tag="OQN12_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1653296.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            30859..33441
                     /gene="leuS"
                     /locus_tag="OQN12_000859"
     CDS             30859..33441
                     /gene="leuS"
                     /locus_tag="OQN12_000859"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1653297.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            33456..34037
                     /gene="lptE"
                     /locus_tag="OQN12_000860"
     CDS             33456..34037
                     /gene="lptE"
                     /locus_tag="OQN12_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1653298.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            34037..35068
                     /gene="holA"
                     /locus_tag="OQN12_000861"
     CDS             34037..35068
                     /gene="holA"
                     /locus_tag="OQN12_000861"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1653299.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            35070..35711
                     /gene="nadD"
                     /locus_tag="OQN12_000862"
     CDS             35070..35711
                     /gene="nadD"
                     /locus_tag="OQN12_000862"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1653300.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            35735..36346
                     /locus_tag="OQN12_000863"
     CDS             35735..36346
                     /locus_tag="OQN12_000863"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCS1653301.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            36606..36923
                     /gene="rsfS"
                     /locus_tag="OQN12_000864"
                     /gene_synonym="rsfA"
     CDS             36606..36923
                     /gene="rsfS"
                     /locus_tag="OQN12_000864"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1653302.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            36927..37394
                     /gene="rlmH"
                     /locus_tag="OQN12_000865"
     CDS             36927..37394
                     /gene="rlmH"
                     /locus_tag="OQN12_000865"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1653303.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            37425..39326
                     /gene="mrdA"
                     /locus_tag="OQN12_000866"
     CDS             37425..39326
                     /gene="mrdA"
                     /locus_tag="OQN12_000866"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1653304.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            39329..40441
                     /gene="mrdB"
                     /locus_tag="OQN12_000867"
                     /gene_synonym="rodA"
     CDS             39329..40441
                     /gene="mrdB"
                     /locus_tag="OQN12_000867"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1653305.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            40452..41540
                     /gene="rlpA"
                     /locus_tag="OQN12_000868"
     CDS             40452..41540
                     /gene="rlpA"
                     /locus_tag="OQN12_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1653306.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            41679..42890
                     /gene="dacA"
                     /locus_tag="OQN12_000869"
     CDS             41679..42890
                     /gene="dacA"
                     /locus_tag="OQN12_000869"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1653307.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            43001..43264
                     /locus_tag="OQN12_000870"
     CDS             43001..43264
                     /locus_tag="OQN12_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1653308.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            43365..44006
                     /gene="lipB"
                     /locus_tag="OQN12_000871"
     CDS             43365..44006
                     /gene="lipB"
                     /locus_tag="OQN12_000871"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1653309.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            44265..45218
                     /locus_tag="OQN12_000872"
     CDS             44265..45218
                     /locus_tag="OQN12_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1653310.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            45427..46392
                     /gene="lipA"
                     /locus_tag="OQN12_000873"
     CDS             45427..46392
                     /gene="lipA"
                     /locus_tag="OQN12_000873"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1653311.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(46493..46696)
                     /gene="tatE"
                     /locus_tag="OQN12_000874"
     CDS             complement(46493..46696)
                     /gene="tatE"
                     /locus_tag="OQN12_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1653312.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(46825..47613)
                     /locus_tag="OQN12_000875"
     CDS             complement(46825..47613)
                     /locus_tag="OQN12_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCS1653313.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            47706..48089
                     /gene="crcB"
                     /locus_tag="OQN12_000876"
     CDS             47706..48089
                     /gene="crcB"
                     /locus_tag="OQN12_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1653314.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(48143..48352)
                     /gene="cspE"
                     /locus_tag="OQN12_000877"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(48143..48352)
                     /gene="cspE"
                     /locus_tag="OQN12_000877"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1653315.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(48527..49087)
                     /gene="pagP"
                     /locus_tag="OQN12_000878"
     CDS             complement(48527..49087)
                     /gene="pagP"
                     /locus_tag="OQN12_000878"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1653316.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            49675..51060
                     /gene="dcuC"
                     /locus_tag="OQN12_000879"
     CDS             49675..51060
                     /gene="dcuC"
                     /locus_tag="OQN12_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1653317.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLDRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(51101..51442)
                     /locus_tag="OQN12_000880"
                     /pseudo
     CDS             complement(51101..51442)
                     /locus_tag="OQN12_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(51780..52289)
                     /locus_tag="OQN12_000881"
     CDS             complement(51780..52289)
                     /locus_tag="OQN12_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1653318.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            52452..52697
                     /locus_tag="OQN12_000882"
     CDS             52452..52697
                     /locus_tag="OQN12_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1653319.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            52672..54303
                     /locus_tag="OQN12_000883"
                     /pseudo
     CDS             52672..54303
                     /locus_tag="OQN12_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        13657 a        13649 c        13589 g        13412 t
ORIGIN      
        1 tgaacaggaa tggtgatgtt atccagtacc cagtcgtaca gacgacggtt gtcctggaac
       61 tcaagcgtac acagagagtg cgtaatacct tccagcgcat cgctgatgca atgggtgaag
      121 tcgtacatcg ggtagatgca ccacttgttg ccagtctggt ggtgttcagc aaatttaatg
      181 cggtacagca ccggatcgcg cattacgata aatggcgacg ccatgtcgat tttcgcacgc
      241 aggcaggctt taccttcttc aaaaccaccg gcacgcattt tttcgaacag cgccaagttc
      301 tcttcaacgc tgcggtcgcg gtacgggctg tttttacccg gctgcgtcag ggtaccgcgg
      361 tattcgcgga tctgttccgg tgtcagttca tcaacgtacg ccaggccttt attgatcagt
      421 tcgatcgcat aggcgtggag ctgatcaaaa taatcggagg agtaacggac gttaccagac
      481 cagtgaaaac ctaaccactc tacgtcgttt ttgatcgaat cgacatactc gatatcttct
      541 tttaccgggt tagtgtcgtc gaaacgcagg ttgcactggc ctttatagtc ctgggcgatc
      601 ccgaagttca ggcagataga tttcgcatgg ccaatatgca gatagccatt cggctccggc
      661 gggaaacggg tgtgtactgt gatgtgctta ccactggcca gatcttcatc gatgatctga
      721 cggataaagt tagtcgggcg ggcttctgcc tcactcatcg tggattcctc aaagcgtaaa
      781 caacgtataa cggcgtatga tcttataagc gggacaggct gacaactgtt agttttttaa
      841 aattgataat ctaacaagga ttatggggat gattgatgaa aaaaaaagcg gcagagaaga
      901 tctccaccgc ttgtcattgt tggatgcgac gctgccgtgt cgcatcaggc ataaagcaga
      961 ttactttttg atttcataca gcggtgtttg acccgccaca acatggccct gagctttaat
     1021 gagcaagcca ctgaaatcgt cgatattgct gcaaaccacc gggctaatca tcgagcgggc
     1081 gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgcgc ttacctgcgc
     1141 accctcttcc accagacgtt taaagccttt accttccagc gctacggtgt cgatacccat
     1201 atggacgacg atctccgcgc ctttttcggt tttcaggcag aacgcgtggt tggtgttgaa
     1261 gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg gtttcaccgc
     1321 cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca ggaacctgat ccagtgccac
     1381 gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg gtacagcctg
     1441 cggttttgct acaggcgcgg cagttgccgg agttgcttca gctgacgcag cggctaccgg
     1501 accacgggca acgactttct tcatcgcatc gccgatggat tctgctttcg cgccaacaat
     1561 cacctgaata gtctgtttgt tcagtttcac tacaccagaa gcacccagac gtttacacat
     1621 cgtatcgttg acgcgggcag agtcagccac ggtcaggcgc agacgggtaa tacaggcatc
     1681 aatcgctttc aggttgtcag taccgccaac cgcagcaata tagttggttg ccagttgagt
     1741 cagaccttct tcagtattgc tgttggcttc ttcagtaacg atctcgtctt ctttatcttc
     1801 acgacccggc gttttcaggt tgaacatgcg gataaccaaa ctgaacacca cgaagtagat
     1861 agcgaagaag ataacgccca tcaccagcag catccagacg ttctggctgg cggcaggcag
     1921 gttatacatc aacgcgtagt cgatagcacc cgcagagaaa gagaagccgg catggatacc
     1981 cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga ggtacagcag
     2041 cggcgcaagg aacatgaaca ggaattccag cggctcagtc acaccggtca ggaacgcagt
     2101 aacagcaaca gaaagcagca tcccgccaac catcggacga cgctctttcg gtgctgcgaa
     2161 gtacatcgcc agcgccgcac ccggcagacc gaacatcatg atcgggaaga agccggacat
     2221 gaacatcccc gcggtgccgt caccagcgta gaaacggttg atgtcgccgt ggaaaaccgt
     2281 acccgccgcg ttggtgaatt caccaatctg gaaccaggcg atggtgttca gcacctgatg
     2341 cagaccggtt gggatcagca gacggttgat gaaaccaaag ataccggaac ccagcgcgcc
     2401 cgcagaaacg atccactcgc cgcctgcatg gatagcgtgc tgtaccggcg gccagacgta
     2461 accaaaaatg gccgccagca ccagacagaa gaagccggtg gcgatcggca caaagcgttt
     2521 gccgccgaag aagctcagga agtccggcag tttaatatcg gaccaacggt tataggctgc
     2581 gccaccaacc agaccggtaa tgatacccgc cagtacaccc atgttaattt ctgggttgat
     2641 ggtcaccatc gctttggtta acacaaagta acctaccgca cccgccagcg ccgccgcacc
     2701 tgcgctgtct ttcgaccagc tggatgccac accgatggcg aagattaatg cgaggttatc
     2761 aaaaattgca ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg gctgaccgaa
     2821 tcgcagcaac agcgccgcca ccggcagcac cgcgataggg agctgtaacg ccctaccgag
     2881 tcgctggaaa aaacctaaaa tattcatctt attcccccta cgagaaccct atttggctcg
     2941 tttaaagccg tatttttatt ttgctgcaaa ttttactgcc gatgttctgt aatcagattg
     3001 ttagatcatc tgctacagag tgtgtgaaaa tttaattcgc atcgcaaatt aaacgcgtgt
     3061 cttttgtgag ttttgtcacc aaatatcgtt atcatcactc ccttttactg gctaaaccag
     3121 aaaacttatt ttatcattca aaaaatcaag tcggattgac gcctgtctgc gcaaatccag
     3181 gttacgctta aagatgtcta atccgccaac ggcttacatt ttacttattg aggtgaataa
     3241 tgagactgat ccccctgact accgctgaac aggtcggcaa atgggctgct cgccatatcg
     3301 tcaatcgtat caatgcgttc aaaccaactg ccgatcgtcc gtttgtactg ggcctgccga
     3361 ctggcggcac gccgatgacc acctataaag cgttagtcga aatgcataaa gcaggccagg
     3421 tcagctttaa gcacgttgtc accttcaaca tggacgaata tgtcggtctg ccgaaagagc
     3481 atccggaaag ctactacagc tttatgcacc gtaatttctt cgatcacgtt gatattccag
     3541 cagaaaacat caaccttctc aacggcaatg ccccggatat cgatgccgag tgccgccagt
     3601 atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt tatgggcggt gtaggtaacg
     3661 acggtcatat tgcatttaac gaaccggcgt cttctctggc ttctcgtact cgtatcaaaa
     3721 ccctgactca tgacactcgc gtcgcgaact ctcgtttctt tgataacgat gtcaatcagg
     3781 tgccaaaata tgccctgact gtcggtgttg gtacactgct ggatgccgaa gaagtgatga
     3841 ttatggtgct gggtagccag aaagcactgg cgctgcaggc cgccgttgaa ggttgcgtga
     3901 accatatgtg gaccatcagc tgtctgcaac tgcatccgaa agcgatcatg gtgtgcgatg
     3961 aaccttccac catggagctg aaagttaaga ctttaagata tttcaatgaa ttagaagcag
     4021 aaaatatcaa aggtctgtaa ttgttatccc tgccctggtt ccttgctcag ggcaatattt
     4081 tttaaaatcg ggggtcagaa tgtatgcatt aacccagggc cggatcttta ccggccacga
     4141 atttcttgat gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg tctgtccggt
     4201 agcggaactg ccgccagaga tcgaacaacg ttcactgaac ggggccattc tctcccccgg
     4261 ttttatcgat gtgcagttaa acggctgcgg cggcgtacag tttaacgaca ccgctgaagc
     4321 ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat gagaaatcag gctgtactaa
     4381 ctatctgccg acgcttatca ccaccagcga tgagctgatg aaacagggcg tgcgcgttat
     4441 gcgtgagtac ctggcaaaac atccgaatca ggcgttaggt ctgcatctgg aaggtccgtg
     4501 gctgaatctg gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc ctgatgccgc
     4561 gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc aaagtgaccc tggcaccgga
     4621 aatggttcct gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg tttctgccgg
     4681 tcactccaac gcgacgttga aagaagcaaa agccggtttc cgcgcgggga ttacctttgc
     4741 cacccatctg tacaacgcga tgccgtatat taccggtcgt gaaccgggcc tggcgggcgc
     4801 gatcctcgac gaagctgaca tttattgcgg tattatcgct gatggcctgc atgttgatta
     4861 cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa ctgtgtctgg ttaccgacgc
     4921 caccgcgcca gcaggtgcca acattgaaca gttcattttt gcgggtaaaa caatatacta
     4981 ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct taaccatgat
     5041 tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca ctggatgaag tgctgcgtat
     5101 ggcgacgctc tatccggcgc gtgcgattga cgttgaaaaa cgtctcggca cgctcgccgc
     5161 aggtaaagta gccaacctga ccgcattcac acctgatttt aaaatcacca agaccatcgt
     5221 taacggtaac gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg acaagctcag
     5281 ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg cctgattgac
     5341 cagtacgggc caatctcgcg gattcagatt gccgagcaaa gccagcttgc ccccgccagc
     5401 gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt tgatcagcag
     5461 gcctccaccg ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa tttccacgca
     5521 atcggcgtac ggcttggtcg tcatgacgcc accatcactc tgtttgatct cagcagcaaa
     5581 gtgctggcag aagaacatta cccgctgccg gaacgtaccc agcagacgct ggaacatgcc
     5641 ctgctgaatg ccattgctca gtttattgat agctaccagc gcaagctgcg cgagctaatc
     5701 gccatttcgg tgatcctgcc agggcttgtt gacccggaca gcggcaaaat tcattacatg
     5761 ccgcatattc aggtagaaaa ctgggggctg gtagaagcac tggaagaacg ttttaaagtg
     5821 acctgtttcg ttggtcacga tatccgtagt ctggcgctgg cggagcacta cttcggtgca
     5881 agtcaggatt gcgaagactc cattctggtg cgtgtccatc gcggaaccgg ggccgggatt
     5941 atctctaacg ggcgcatttt tattggccgc aacggtaacg tcggtgaaat tggccatatt
     6001 caggtcgaac cgctgggtga acgctgccac tgcggcaact ttggctgcct ggaaactatc
     6061 gccgccaacg ctgccattga acaacgggtg ttgaatctgt taaagcaggg ctaccagagc
     6121 cgtgtgccac tggacgactg caccatcaaa actatctgca aagccgcgaa caaaggcgat
     6181 agcctggcct cggaagtgat tgagtatgtc ggtcgtcatc tgggtaaaac catcgccatt
     6241 gctatcaact tatttaatcc gcaaaaaatt gttattgctg gtgaaatcac cgaagccgat
     6301 aaagtgctgc tccctgctat tgaaagctgc attaataccc aagcgctgaa ggcgtttcgt
     6361 actaatctgc cggtggtacg ttctgagctg gatcaccgct cggcaatcgg cgcttttgcg
     6421 ctggtaaaac gcgccatgct caacggtatt ttgctccagc atttgctgga aaattaatgt
     6481 gcttttatag tggcgcttat tgttgtcaat attctgggta gtccatgacc attaaaaatg
     6541 taatttgcga tatcgacggc gtgctgatgc acgataacgt cgccgtaccg ggtgcagcgg
     6601 aatttttgca cgggattatg gataaaggcc tgccgctggt gttgctgacc aactatcctt
     6661 cgcagactgg gcaagatctg gcgaaccgct ttgccactgc cggtgtcgat gtgccggata
     6721 gcgtgtttta tacctctgcg atggcgaccg ccgatttcct gcgtcgccag gaaggcaaga
     6781 aagcgtatgt ggtgggcgaa ggcgcactga ttcatgaact gtacaaagcc ggtttcacta
     6841 ttaccgatgt gaaccctgat ttcgtgattg ttggcgaaac acgttcctac aactgggaca
     6901 tgatgcataa agcagcctat ttcgtcgcta acggtgcacg ctttatcgcc accaacccgg
     6961 acacccacgg gcgcggtttt tatcccgctt gtggcgcgtt gtgtgcaggg attgagaaaa
     7021 tctccgggcg caaaccgttc tatgttggta agcccagccc gtggatcatc cgcgcggcat
     7081 taaacaaaat gcaggcgcat tcggaagaaa cagtgattgt cggcgataac ctgcgtaccg
     7141 atattctggc gggcttccag gccggtctgg agacgattct ggtgctttct ggtgtttcat
     7201 cgctcgacga tatcgacagt atgccgttcc gcccaagctg gatttacccg tcagtcgctg
     7261 aaatcgacgt tatctgaata ccacccacgt ctgcggacgt ggttggctct gatgacaaac
     7321 gccaaactgc ctgatgcgct acgcttatca ggcctacgca gctcctgcaa tatattgaat
     7381 tttcatgctt ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaatcgca
     7441 ctttatcagc aatttggccc ccgtaaaata taaccattca ataaaacccg ctaaaaatta
     7501 tcgattcatc aataaataca tcctcacacc agaccctttt tcaccaatcg acaatcacca
     7561 ttacgttttt tatttttgca ctgccaaata gcttgcgcac agtgctgctt tgcggcattt
     7621 ttttaaacaa gcaaacacaa caagcaacaa ataccaggtt aacggagaag gttatgtgtt
     7681 caatttttgg cgtattcgat atcaaaacag acgcagttga gctgcgtaag aaagcactcg
     7741 agctgtcacg cctgatgcgt catcgtggcc cggactggtc cggtatttat gccagcgata
     7801 acgccattct cgctcacgaa cgtctgtcaa ttgttgacgt taacgcgggg gcgcaacctc
     7861 tctacaacca acaaaaaaac cacgtactgg cggtaaacgg tgaaatctac aaccaccagg
     7921 cattgcgcgc tgaatatggc gatcgttatc agttccagac agggtctgac tgtgaagtga
     7981 tcctcgcgct gtatcaggaa aaagggccgg aatttctcga cgacttgcag ggcatgtttg
     8041 cctttgccct gtacgacagc gaaaaagatg cctacctgat tggtcgcgac catctgggga
     8101 tcatcccact gtatatgggg tatgacgaac acggtcagct gtatgtggcc tcagaaatga
     8161 aatcgctggt gccagtttgc cgcacgatta aagagttccc ggcggggagc tatttgtgga
     8221 gtcaggacgg cgaaatccgt tcttattatc atcgcgactg gttcgacttt gatgcagtga
     8281 aagataacgt gaccgacaaa aacgagctgc gtcaggcact ggaagattcc gttaaaagcc
     8341 atctgatgtc tgatgtgcct tacggtgtgc tactttctgg tggtctggat tcctcaatta
     8401 tttccgctat caccaagaaa tacgcagccc gtcgcgtgga agatcaggaa cgctctgaag
     8461 cctggtggcc gcagttacac tcctttgctg taggtctgcc gggttcaccg gatcttaaag
     8521 cagcccagga agtggcaaac catctgggca cggtgcatca cgaaattcac ttcactgtac
     8581 aggaaggtct ggatgccatc cgcgacgtga tttaccacat cgaaacttat gatgtgacca
     8641 ctattcgcgc ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggcg atgggcatta
     8701 aaatggtgct gtccggtgaa ggttctgatg aagtgtttgg cggttatctt tacttccata
     8761 aagcgcccaa cgctaaagaa ctgcatgaag agacggtgcg taaactgctg gccttgcata
     8821 tgtatgactg cgcgcgcgcc aacaaagcga tgtcagcctg gggcgtggaa gcacgcgttc
     8881 cgttcctcga caaaaaattc cttgatgtgg cgatgcgcat taacccgcag gataaaatgt
     8941 gcggtaacgg caaaatggaa aaacacatcc tgcgtgaatg ttttgagtca tacctgcccg
     9001 caagcgtggc ctggcggcag aaagagcagt tctccgatgg cgtcggttac agttggatcg
     9061 acaccctgaa agaagtggcg gcgcagcagg tttctgatca gcaactggaa actgcccgct
     9121 tccgcttccc gtacaacacg ccgacctcaa aagaagcgta tctgtaccgc gagatctttg
     9181 aagaactgtt cccacttccg agtgccgctg agtgcgtgcc gggcggtcct tccgtcgcgt
     9241 gttcttcagc taaagcgatc gaatgggatg aagcgttcaa aaaaatggac gatccgtctg
     9301 gtcgtgcggt tggcgttcac cagtcggcgt ataagtaaga aatgcggtac tgccccggag
     9361 aaatccgggg ctgttttagt tattgcgctg cttgttgatt ctgatatgca gtaaggaaat
     9421 gttccgggtt tgccacatca ctgtttgaca actcatcact gtaggccagt atttcataga
     9481 ttaatttccc accactattg ttgaattccg aatttcatcc tgtacgacaa aagaatggct
     9541 gtagggtggg gaaaaacaca aagtaaacaa taattgacga atatagcgcc acgctgttcg
     9601 caacctaacc aaacagtcac tttcgagcaa ttttccttga aaaagaggtt gacgctgcaa
     9661 ggctctatac gcataatgcg ccccgcaacg ccgataaggt atcgcgaaaa aaagatggct
     9721 acgtagctca gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc
     9781 gtcgtagcca ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt
     9841 tctggcgccg caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg
     9901 ttgtacggat ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg
     9961 gttcgaatcc aggtacccca gccatcttct tcgagtaagc ggttcaccgc ccggttattg
    10021 gggtatcgcc aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca
    10081 ggtactccag ccatcgaaga aataatctgg ctacgtagct cagttggtta gagcacatca
    10141 ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc
    10201 gaatacgttc ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc
    10261 ggattctgat tccggcattc cgaggttcga atcctcgtac cccagccaat ttattcaaga
    10321 cgcttacatc gtaagtgcac ccagttgggg tatcgccaag cggtaaggca ccggattctg
    10381 attccggcat tccgaggttc gaatcctcgt accccagcca cattaaaaaa gctcgcttcg
    10441 gcgagctttt tgcttttctg cgttcattca atgtcgaatg cgatgttgac acgtcttatc
    10501 cttcaatgtc ggatgcgacg ctgccgcgtc ttatccgacc tacggctggc acgcatccgg
    10561 caatgttgta aggctacaac cctaacgcat atttcagcgc ctgacgtttc aacacgccag
    10621 cacgctctgc cgccattaac ccgagattac gcacaaaacg cagcggcggc agattattac
    10681 tgaatccggc ataaaacaga tccataccac tttgcataat gaagttatcc gccatgcgcc
    10741 gcatctggta acgcttgagg acaggataac tggcccacgc ttcaccgtag ctgcgggcat
    10801 tcaccagaac atcaatcagg gcatcgacat cacgataacc aagattcacc ccctgccccg
    10861 ccagcggatg gatggtatgc gcggcatcgc ccaccagcgc aagccccggc tgcacatact
    10921 gcaacgcatg tcggcgcgtc agcggaaacg caccagcggc aagcggtgta acgtaaccca
    10981 gacgcgacgg gaaatgcttc gcgatttccg cctggagttg cgccatattc atattctgca
    11041 actggcgaat acgcgccgga gagtcatacc acaccagcga tgcccagtta tcaaacaacg
    11101 gcagaaacgc acgcggtccg tccggggtaa attgctgcca ggtgctgtcg ccgggatcgt
    11161 tctcacactg tacgctaatc aacatgcacg actgcgcata ctgccaggca tgaacgccaa
    11221 ttcccgccat ctgccgcacc tgagaatttg cgccgtcggc accaatcacc agtttcgcgc
    11281 ggatcgtttc accgcccttc agttccagct cctgaagatc attatctcga tgtaatgcaa
    11341 tcagcgaggt tggcacacgt aacgttactt tcggatgcgc ttccagcgcc tgccacaacg
    11401 cctgttgcag gactgtgttt tccaccatgt agccaagcaa cggcaacttt aattcggctg
    11461 cgtcaaatac cacatgtgcc gtttcccact cccaggtttc cagtctgcgg taaggatggc
    11521 aacgcatagc ctgtactgca tcccagaccc ctaacccttt aagcaatgat accgaagccg
    11581 cgctgatcgc cgagatccgc acgtccggtt ggctgtcagc gacaaacggt gcaggttctg
    11641 cgtgctcgat caccgttacc gaaaatccgt gctgtgccag ccccagcgcc agtgcgccgc
    11701 cgaccattcc tccgccgaca atggcaattt ccgttggttg atttgtcatg gtcaatcatc
    11761 ctgttagcaa tatgctttaa gtttacagga tttttacccc caagggctga taacgctcat
    11821 actggtcaca acggcagcaa agcattacac tatgcgccct gcattcctgg ctactatttc
    11881 gcaagagcaa gtcgatgacc aaaaaactcc atattaaaac ctggggctgt cagatgaacg
    11941 agtacgattc atcgaagatg gccgatctgc tggatgccac ccacggctat caactgaccg
    12001 acgtggcgga agaagcggat gtgctactgc tgaacacctg ctcaatccgc gagaaggctc
    12061 aggaaaaagt cttccatcag ttgggtcgct ggaaactgtt aaaagagaag aatccagacc
    12121 tgattatcgg cgtcggtggc tgcgtggcat cgcaagaagg cgagcacatt cgccagcgcg
    12181 cccactatgt cgatattatt tttgggccgc aaacgctgca ccgtctgccg gagatgatca
    12241 actccgtgcg cggcgaccgc agcccggttg tagatatcag cttcccggaa atcgagaagt
    12301 ttgaccgtct gccggaaccg cgcgccgaag ggccgaccgc gtttgtctcc atcatggaag
    12361 gctgcaataa atattgcacc tactgcgtgg tgccttacac ccgtggtgaa gaggtaagcc
    12421 gtccgtctga cgatattctg tttgagattg cccagcttgc ggctcagggc gtgcgtgaag
    12481 tcaacctgct cggtcagaat gtgaatgcct ggcgtggtga gaactacgac ggcaccaccg
    12541 gatcgtttgc cgatctgttg cgtctggttg ctgcgatcga cgggatcgat cgtattcgct
    12601 ttaccaccag ccatccgatc gaattcaccg acgatatcat cgaagtgtac cgcgacacgc
    12661 ctgagctggt gagcttcctg catctgccgg tacagagcgg ttccgatcgc attctgaacc
    12721 tgatgggacg tacccatacg gcgctggagt acaaagcgat catccgtaaa ctgcgtgcgg
    12781 cgcgtccgga tattcagatc agttctgact tcattgttgg cttccctggc gaaaccaccg
    12841 aagacttcga gaaaacgatg aagctgattg ccgacgtcaa tttcgacatg agctacagct
    12901 ttatcttctc tgcacgtcca ggaacgcctg ccgccgatat ggttgatgat gttccggaag
    12961 aagagaagaa gcagcgtctg tatattctgc aagaacgtat caatcagcaa gcgatggcat
    13021 ggagccgtcg gatgctcggc accacccagc gtattctggt agaagggact tcgcgtaaga
    13081 gcatcatgga gctttccggg cgtaccgaaa ataaccgcgt ggtcaacttc gagggcacac
    13141 cggatatgat cggtaaattc gtcgatgtag aaattaccga cgtctacccg aactctctgc
    13201 gcggtaaagt ggtgcgaact gaagatgaaa tgggtctgcg catggcagaa acaccggagt
    13261 cggtgattgc ccgcacccgc aaagaaaacg accttggcgt gggttattat cagccgtaat
    13321 tctcaggcct gccgttccgg tgggccttgt tttttctctc tttatcccca tctttatcgc
    13381 aatgcttgcg cctttttcct gtggcgtgaa taatttcctt ataggccggt gaatgttcag
    13441 cttccggcgg catacagata agaggaacgg tttgaacata gacactcgcg aaatcaccct
    13501 ggagccagca gacaatgcgc gtctgttgag cctgtgcggc ccgtttgatg acaacatcaa
    13561 gcagctcgaa cgccgtctcg gcatcgagat caatcgccgc gataaccact ttaaactgac
    13621 cggccgtccg atttgcgtca ccgctgcggc agacattctg cgtagcctgt atgtcgatac
    13681 tgccccgatg cgcggtcaga ttcaggatat cgaaccggaa cagatccacc ttgcgattaa
    13741 agaagcgcgg gtactggagc aaagcgcgga gagcgtgccg gagtacggca aagcggtcaa
    13801 tatcaaaact aaacgcggcg taattaagcc gcgcacgcca aaccaggcgc agtacatcgc
    13861 caatattctc gaccatgaca ttaccttcgg cgttggcccg gcgggtacgg gtaaaaccta
    13921 cctggcagtg gctgcggcag ttgatgccct ggagcgtcag gaaattcgcc gtattctgct
    13981 gactcgtccg gcggtcgaag ccggtgagaa actgggcttc ctgcctggcg atttaagcca
    14041 gaaagtagac ccgtatctgc gcccactgta cgacgcgctg tttgaaatgc tgggctttga
    14101 gaaagtcgag aaactgattg agcgcaacgt tattgaagtc gcgccgctgg cctatatgcg
    14161 tggtcgtacg ctgaacgacg cgtttatcat tctcgatgag agccagaaca ctaccatcga
    14221 acagatgaag atgttcctga cccgtatcgg ttttaactca aaagcggtta tcaccggcga
    14281 cgtcacacag atcgacctgc cgcgtaatac taaatcaggc ttacgtcatg ccattgaagt
    14341 gctggccgat gtcgaagaga tcagctttaa cttcttccac agcgaagacg tggttcgtca
    14401 cccggtggtg gcgcgtatcg ttaacgctta tgaagcctgg gaagaagcag aacaaaaacg
    14461 taaagcggcg ctggcagcag aacgcaagcg cgaagaacag gaacaaaaat gagtcaggtg
    14521 atcctcgatt tacaactggc atgtgaagat aattccgggt taccggaaga gagccagttt
    14581 caaacatggc tgaatgcggt gatcccgcag tttcaggaag agtcggaagt gactattcgc
    14641 gtggtcgata ccgccgaaag ccacagtctg aatctgacct atcgcggtaa ggataagccg
    14701 accaacgtgc tctccttccc gtttgaagtg ccgcctggca tggagatgtc gctactgggc
    14761 gatctggtta tctgccgtca ggtggttgag aaggaagctc aggagcaagg caaaccactg
    14821 gaggcgcatt gggcgcatat ggtggtgcac ggcagtctgc atttgttagg ttacgatcac
    14881 attgaagatg acgaagcaga agaaatggaa gccctcgaaa cagagattat gcttgctctg
    14941 ggctatgagg atccgtacat tgccgagaaa gaataatttg ccaggcattt gactggcaga
    15001 cgtccccgcc gttggtgatt aacgacggcg ttttgattaa ctaacatgag aacccatacg
    15061 acgccatgag cgacgacaat tcacacagta gtgacacgat aagcaacaag aagggatttt
    15121 tctccctgtt actcagccaa cttttccacg gtgaaccgaa aaaccgtgac gaactgctgg
    15181 cgctgatccg tgattccggg cagaacgacc ttatcgacga agatacgcgc gatatgctcg
    15241 aaggggtgat ggacatcgca gaccaacgcg ttcgcgacat catgatcccc cgctcccaga
    15301 tgattaccct gaaacgcaac cagacgctgg acgaatgcct tgatgtcatc atcgagtccg
    15361 cccactcacg tttcccggtg attagcgaag acaaagatca cattgaaggg attctgatgg
    15421 cgaaagactt gctgccgttt atgcgcagcg atgctgaagc cttcagcatg gacaaagtgt
    15481 tacgtcaggc ggttgtcgtt cctgaaagta agcgcgtaga ccggatgctg aaagagtttc
    15541 gctctcagcg ttaccacatg gcgatcgtta ttgacgaatt cggtggggtt tccggtctgg
    15601 tgaccattga agacatcctg gaactgattg ttggtgagat tgaagacgag tatgacgaag
    15661 aagatgatat cgacttccgt cagctgagtc gtcatacctg gaccgtgcgc gcactggctt
    15721 ccattgaaga cttcaacgaa gcgttcggca cccactttag cgatgaagaa gtcgacacta
    15781 tcggtggtct ggtgatgcag gcgtttgggc atcttccggc gcgtggcgaa actatcgaca
    15841 tcgacggtta ccagttcaaa gtggcgatgg ccgatagtcg gcgtattatt caggttcatg
    15901 tcaaaatccc ggatgactca ccccagccaa agctggatga ataaaaccga aactggatag
    15961 ataactacat ggcttttgcc tcattaattg aacgccagcg tattcgcctg ctgctggcgt
    16021 tattattcgg tgcctgcgga acgctggcct tctctcctta cgacgtctgg cctgcggcga
    16081 ttatttcgct gatggggctt caggcgttga cctttaaccg tcgcccactc cagtctgccg
    16141 ctattggctt ttgctgggga tttggcctct ttggcagcgg tattaactgg gtctatgtca
    16201 gcatcgcgac ctttggcggg atgcctggcc cggttaacat cttcctggtg gtgctgctgg
    16261 cggcgtattt gtcgctgtat accggactgt ttgctggcgt gctgtcgcgt ctgtggccga
    16321 aaaccacctg gctgcgcgtg gcgattgcca cccctgctct ctggcaagtg accgagtttc
    16381 tgcgcggttg ggtgctgact ggcttcccgt ggttacagtt cggctatagc cagattgatg
    16441 gcccgttaaa agggctggca ccgctaatgg gcgtggaagc cattaacttc ctgctgatga
    16501 tggttagtgg cctgctggca ctggcgttgg tcaaacgcaa ctggcgtccg ctggtggtgg
    16561 ccgtcgtgct gtttgcccta cccttcccgc tgcgttacat ccaatggttt accccgcaac
    16621 cggagaaaac cattcaggtt tcgatggttc agggcgatat tccgcaatcg ctgaaatggg
    16681 acgaaggcca gcttcttaat acgctaaaga tttactacaa cgcaacggca ccgctgatgg
    16741 gcaaatcatc gttgattatc tggccggagt cggcgataac cgatctggaa attaatcagc
    16801 aaccgttcct caaagcactg gacggtgagt tgcgtgataa aggtagctcg ctggtaaccg
    16861 ggattgtcga cgcgcgtctc aataagcaga accgctacga tacctacaac accatcatca
    16921 cgctgggtaa aggtgcaccg tacagctacg aatcagccga tcgctataac aaaaaccatc
    16981 tggtgccgtt tggcgagttt gtcccgctgg agtcgattct gcgtccgtta gcaccgttct
    17041 ttgatctgcc gatgtcgtcg ttcagccgtg ggccatatat ccagccgccg ctgtcggcaa
    17101 atggtattga gcttactgcg gctatttgct acgagatcat tctcgccgag caagtgcgcg
    17161 ataacttccg cccggatacc gactacctgc tgactatctc caacgatgcg tggtttggta
    17221 aatctattgg tccatggcaa cacttccaga tggcgcgaat gcgtgcgctg gagctggcgc
    17281 gcccactgtt gcgcagcacc aacaacggca ttacggcggt gattggcccg cagggtgaga
    17341 ttcaggcgat gatcccgcag ttcgcccgcg aggtgttaac cactaacgtg acgccgacca
    17401 ccggactcac accatacgca cgtaccggca actggccgct gtgggtgctg acggcactgt
    17461 ttggttttgc tgctgtgttg atgagtctgc gtcagcgacg taaataatcc cttcttatcg
    17521 tgccggaata catcccatat ttcggcacgc tcaccggatg cggcatcagt gcattatccg
    17581 gataacaata ttctcctatc caactttggc acatctattg ctttgttata caaggcaaag
    17641 cttgaaccag catcagtaca acccggttgc accacagcaa tccaccggta gcaccaaaag
    17701 agtgcatttt aagaattttg cttcagaatg gtgcggtgca cctcgcaaaa acaaacaata
    17761 aaacatcaat atctttacat ttagccaaac taaatgttac ctaacaggca caacactgca
    17821 caataaagtt gcagacgata acaacacaaa cactcacaac gggtatccat gcgttcttaa
    17881 cgcagaagat aaaggtgttg gatatgcaat tacgtaaacc tgccacagca atcctcgccc
    17941 tggcgctttc cgcaggactg gcacaggcag atgacgccgc cccggcagca ggcagtacgc
    18001 tggacaaaat cgccaaaaac ggtgtgattg tcgtcggtca ccgtgaatct tcagtacctt
    18061 tctcttatta cgacaatcag caaaaagtgg tgggttactc gcaggattac tccaacgcca
    18121 ttgttgaagc agtgaaaaag aaactcaaca aaccggactt gcaggtaaaa ctgattccga
    18181 ttacctcaca aaaccgtatt ccactgctgc aaaacggcac tttcgatttt gaatgtggtt
    18241 ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt ctctgacact attttcgtgg
    18301 tcggtacgcg cctgttgacc aaaaagggcg gcgatatcaa agattttgcc gacctgaaag
    18361 gcaaagccgt agtcgtcact tccggcacta cctctgaagt tttgctcaac aaactgaatg
    18421 aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac tctttccgca
    18481 ccctggaaag cggtcgtgcc gttgccttta tgatggatga cgctctgctg gccggtgaac
    18541 gtgcgaaagc gaagaaacca gacaactggg atatcgtcgg caagccgcag tctcaggaag
    18601 cctacggttg catgttgcgt aaagacgatc cgcagttcaa aaagctgatg gatgacacta
    18661 tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataaatgg ttcaaaaatc
    18721 caattccgcc gaaaaacctg aacatgaatt tcgaactgtc agacgaaatg aaagcactgt
    18781 tcaaagaacc gaatgacaag gcactgaact aattacaaga accaggggcg gaaattccag
    18841 ccctctcgat tgttacgtag cacggacaga ctatacgcct gatggtcgtt ccccatcggg
    18901 cctgaaaacc gcaatacgct gggtaacaat cttcgagggt agcagttaac gctgctaccc
    18961 tcttttttct ggagtagatt tatgtctata gactggaact ggggtatttt tttacaacaa
    19021 gccccgttcg gcaataccac ctatcttggt tgggtctgga gtggttttca ggtgacgatc
    19081 gctttgtcga tctgcgcctg gattatcgct ttcctcgtcg gttcattttt cggcatttta
    19141 cgtaccgttc caaaccgttt tctctccggt ctgggtacgt tgtatgtcga actgttccgc
    19201 aacgtgccgt tgattgtgca attctttacc tggtatctgg tgatcccgga gctgctgccg
    19261 gagaaaatcg gcatgtggtt taaggccgag ctggatccca atattcagtt tttcctttca
    19321 tccatgctct gcctggggct gtttaccgcc gcccgtgttt gcgaacaggt acgcgccgct
    19381 attcagtcgc taccgcgtgg gcagaagaat gccgcgctgg cgatgggcct gacgctaccg
    19441 cagacttatc gatacgtact gctgcctaat gcttatcgcg ttatcgtccc gccgatgact
    19501 tccgagatga tgaacctggt gaaaaactcc gccatcgcct cgaccatcgg tctggtggat
    19561 atggcggcgc aggcgggtaa attgctcgac tactcggctc acgcctggga gtcgtttacc
    19621 gccatcacgc ttgcgtatgt gttgattaac gcttttatca tgctggtgat gacgctggtt
    19681 gaacgtaaag ttcgcctgcc cggcaacatg gggggcaaat aatgtacgag tttgactgga
    19741 gttccattgt tccttccctg ccatatctgc tcgacgggct ggtgatcacc ctgaaaatca
    19801 ccgtcacggc agtcgtgatt ggtattttgt ggggtaccat gctggcggtg atgcgcttgt
    19861 ccagcttcgc gcctgtttcg tggtttgcca aagcctacgt taacgtattc cgctcgattc
    19921 ctttagtcat ggttttgctg tggttttact tgattgtgcc gggatttctg caaaatgtgc
    19981 tgggattatc gccaaaaaat gatattcgtt taatttcggc gatggtggcg ttttcgatgt
    20041 ttgaagcggc ctactattca gagattatcc gtgccggtat tcaaagtatt tcccgtggtc
    20101 agtcgagcgc cgcgctggcg ttggggatga ctcactggca gtcgatgaaa ctgattattc
    20161 tgccgcaggc tttccgcgcg atggtgccgc tgctgctcac tcagggcatc gtactgttcc
    20221 aggatacctc gctggtgtat gtgttatctc tggccgattt cttccgtacc gcctcaacca
    20281 ttggtgagcg tgatggtacg caggtcgaga tgatcctgtt tgccggattt gtttatttcg
    20341 ttattagcct tagcgcgtcg ttgttggtca gctacttgaa aagaaggaca gcataatgat
    20401 taccctgaaa aatgtttcaa aatggtatgg tcactttcag gtgctgaccg actgctcaac
    20461 cgaagtgaaa aaaggcgaag tggtggtggt ttgcggcccg tctggttccg gcaaatcaac
    20521 gctgattaaa accgtcaacg gcctcgaacc agtgcagcaa ggtgaaatca ccgtcgatgg
    20581 tatcgtggtt aacgacaaga aaaccgatct ggcaaagctg cgctcccgcg tcgggatggt
    20641 attccagcat ttcgagctgt tccctcatct gtcgattatc gaaaacctga ccctggcgca
    20701 ggtgaaagtg cttaaacgcg ataaagcgcc cgctcgtgaa aaagcattga aattgctgga
    20761 gcgcgtggga ctttccgcac atgccaataa atttccggcg cagctttccg gcggtcagca
    20821 gcagcgcgtg gcaatcgctc gcgcattgtg tatggctcct attgcgatgc tgttcgacga
    20881 accgacatcg gcgctggatc cggagatgat caacgaagtg ctggacgtga tggtggaact
    20941 ggcgaacgaa ggaatgacca tgatggtggt gacccacgaa atgggctttg cccgcaaagt
    21001 ggcgaatcgg gtgatcttta tggacgaggg taaaattgtc gaagactcgc cgaaagacgc
    21061 tttcttcgat gatccgaaat cggaccgcgc aaaagacttc ctcgcgaaaa tcctgcatta
    21121 atcactctgg cgcacatacc caattgcgcg ccatctcacg ctttatcgtt tcgcccctcg
    21181 tatctgttac atcctggcgt tacccttgtc gcaaagaagc acacaacaag gagcaacaat
    21241 ggcactgcca attctgttag attgcgaccc aggtcatgat gacgctatcg caatagttct
    21301 cgcactcgcc tcaccagagc ttgatgtcaa agcaattacg tcttccgccg gaaaccagac
    21361 accagaaaaa accttacgca atgttctgcg tatgctgacc ttgcttaatc gcaccgatat
    21421 tccggtggca ggcggcgcgg taaaaccgtt aatgcgtgag ttgattatcg cggacaatgt
    21481 gcacggcgaa agcggtctcg acggcccggc attaccggaa ccgacgttcg caccgcaaaa
    21541 ctgtacggcg gtggagctga tggcgaaaac gctgcgcgaa agtgcggaac ctgtcacgat
    21601 tgtttctacc ggaccgcaaa ctaacgttgc cttgctgctc aatagccatc cggaactgca
    21661 tagcaaaatt gcccgtatcg tgattatggg cggcgcaatg gggcttggta actggacgcc
    21721 tgcggctgaa tttaatattt acgttgaccc ggaagcggca gaaattgtct tccagtcagg
    21781 gatcccggtg gtgatggccg gtctggatgt tactcataaa gcgcaaatcc acgttgaaga
    21841 caccgagcgt ttccgcgcga ttggcaaccc tgtttcaacc attgttgccg aactgctgga
    21901 tttcttcctc gaatatcata aagacgaaaa atggggcttt gtcggcgcac cgctgcatga
    21961 cccatgcacc atcgcctggc tgttgaaacc cgagttgttt accactgttg agcgctgggt
    22021 tggcgtggaa acacagggga aatataccca gggtatgacg gttgttgatt attattatct
    22081 gactggcaat aaaccgaatg ccactgtaat tgtcgatgtt gatcgtcagg gctttgttga
    22141 tttactggct gatcgtctga aattttacgc ttaacactcg tcattgatta agatcaccac
    22201 gcgttacctg ggtggtctta atctatttat ctctttttct ttcaagatat aaatgcaatg
    22261 gataatgcag aactcgccat aggtatcgat ctcggcacta ccaatagttt aattgccgtc
    22321 tggaaagacg gtgccgcgca attaattcca aataagttcg gtgaatattt aacaccatcc
    22381 ataattagca tggatgaaaa taatcatatt ttagtcggaa aaccggctgt atcacggcgt
    22441 acttcgcatc cggataaaac ggcagcgtta tttaaacgtg caatgggcag ctggcggtta
    22501 ggcagcgaca catttaacgc gccagaactg tcctctttgg tattacgctc attaaaagaa
    22561 gatgccgaag aatttctgca acgtccgatt aaagatgtgg tgatctccgt tccggcttat
    22621 ttcagtgatg aacaacgcaa gcatacccgt ttagcagcgg acttagccgg gttaaatgcg
    22681 gtacgcttaa ttaatgaacc cacagcagct gcgatggcgt atggcctgca tacccaacaa
    22741 aatacccgtt cgctggtgtt tgatctcggt ggcggcacgt ttgacgttac ggtgcttgag
    22801 tacgccacgc cggtgattga agttcacgcc tccgctggcg acaacttcct cggtggcgaa
    22861 gattttaccc atatgttggt cgatgacgtt ttaaagcgcg cggatgtcac aaggaccacg
    22921 cttaacgaga gtgaactggc agccttgtac gcctgtgtgg aagcggcaaa atgtagcaat
    22981 caatcgccaa tgcacattcg ctggtagtac caggatgaaa cgcgggaatg cgaattttac
    23041 gagaacgaac tggaagattt gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa
    23101 caggcgttgc gcgatgcgcg tctgaagccg agtcaaatcg acagtctggt gctggttggc
    23161 ggcgcgtcac aaatgccgct ggtgcagcga atcgccgtgc gtctgtttgg caaattaccg
    23221 tatcaaagtt acgatccgag caccattgtc gcgctgggcg cagcaatcca ggccgcctgc
    23281 cgcttacgca gtgaagatat tgaagaagtg atcctcactg atatttgccc ttactcgttg
    23341 ggcgttgagg ttaaccgcca gggcgtttcc ggcattttct cgccgattat tgaacgaaac
    23401 accactgtgc ccgtgtcgcg tgtagaaact tattcaacca tgcacccgga acaggattca
    23461 attacggtta acgtctatca gggagaaaac cacaaagtta aaaacaacat tctggtggaa
    23521 tccttcgatg tgccgttgaa gaaaaccggg gcttatcagt cgattgatat tcgctttagt
    23581 tatgatatca acgggttgct tgaagttgac gtgcttctgg aagacggcag cgttaagtcc
    23641 agagtgatta accacagccc ggtaacattg agcgcgcagc agattgaaga gagtcggacg
    23701 cggttatccg cattgaaaat ttatccgcgc gatatgctca tcaatcgcac ctttaaagcc
    23761 aaactggaag agttgtgggc gcgggcgctg ggtgacgagc gagaagagat tggccgggtg
    23821 atcaccgatt ttgatgcggc gctgcagtca aacgatatgg cccgcgtcga tgaggtcagg
    23881 cggcgggcga gcgtttattt agccattgag acctcataat cacggctgcc ggatgcggcg
    23941 taaatgctgc atccggcatc ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt
    24001 ttgccgagag cataacctat aaccagcacc gttaaactga ttttgataat gctggtaatg
    24061 ctctgtggtt tttcaccctc atcatttttt tcttcatggt tttcaggtgt tggctcatcg
    24121 acatcttcat cctccggctc gagttttact ttcgtcaaca gcatccgggt gataatggcc
    24181 tctaatggat gagcatctcc gccttccagc cttgtattaa gacgggccag catctcttgt
    24241 ttcggaaacg cttcaagtac tacctgttcc gctacccgat acaaatccag taacggtgaa
    24301 tcttcttctg tctcgctacc gactctcgat ttccaccacg cccatgcttt gcgtttgaag
    24361 aataacgact caataaacga ttgacctaac ggcgataatt ttccgctatg taaaatttcc
    24421 gcccataaca gccgcgtccg ggcgctctca ccaggaacat atttcagcgg ttgaccttgc
    24481 gcatctacca tgctccgcgc ttcgataacc atcactaata acgcgaaata atccgggcaa
    24541 tgacgcttgc tccactgcaa caacaaatta tcagcttgtg tagatgggta actttcccag
    24601 tacgcgccga gatcagcaag caggctttcc ccctcgccgc agtacaaacg ttgtgcacac
    24661 tggtaatacc gggcatcttc gctttctggt tcttccgctt gccactgttt ggctacagga
    24721 atgagttcca ccatacctgt ttgcaccgag ctgtaccagc gcaacagttt gcgatgtaac
    24781 tttgcatcat caggaatgaa ccacggtcct tccatcgcca aaaacgcagt gacatagtca
    24841 ggatggtaac ggcagcaacg ttccagcgcg aagtaatatt caacagtctg gttctgtaac
    24901 gcgacgggat attccagaag gctaaggaaa tcgaaacaat caccagcctg aatggcctca
    24961 agaaaagcct ccctttcttc ctcatccatt ccttcgctgt cattaaacga atgccagttc
    25021 aaacgctctg ccaacaaaga agcacaattt aatgacagat atcgcgcttc tatagcgatt
    25081 gcacacagcg gccagcgtaa ttgatcgaca tcctccatgt tccaggtatt taattgctga
    25141 ataaatttct gccaggcgga gggctgaaaa cgatcacttt ctgaatccag taatgtcctg
    25201 aatgcacgta gtatttcatg ttcagcggtg gcatcttttt cttcatcatc tgcttcctcg
    25261 acaggattta ccgccagtcg cagcgcctct tcataggcct ggcgtaatgc tttaaacccc
    25321 tgcggatcgg tttcgggatg acacaacggt aagcgagcaa gataagcctg gcggataatg
    25381 tctatttgcg tcgtgctttc aatttcgagg atttgccaac atgttttcat aggtcgctca
    25441 catccttaag cgttaccggg cattcaggct ggacagttaa tggctgccag ggaagcgtgg
    25501 catagaaacg gtcgcttccc gctgccagct cttcgaacat ccgactcccg gcgctatcgt
    25561 atttgtacag ggttagcgcc tcgcgtagcg aagcgttgtc ttcgcactga gaagattgcc
    25621 agtagagacg ccctagtgaa taggcagaaa aatagtgcat ccagctgtga tagtaatggt
    25681 gagctcgtgc atagacgcgc gattgcagcc ataaactctc ttcttccgtc agccagttgt
    25741 tgagcacacc ggcccgtgac agaaacccca ttcgaacata atcccaggct ttgattcccc
    25801 cttctccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
    25861 agccaccagc aattaatgct ttccactctt caggggaaga gcgttgccat gtcagatata
    25921 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
    25981 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttatc ccggtataca
    26041 tttttggcga gtcatgcgcg ggtttgcaac tctgattaag tatttccatg ggcgcagaaa
    26101 gcgcaaaaag aagatattgt tcttccttat tcatttttta ataatccctt aagtgcatta
    26161 actgactact tttttattat tgatgagtat aaacttgacg ctttcaaaat aaaaagaaaa
    26221 tcgaagcatt cacacatgaa taaaaaatta atgtatatat tcgcaatttt tatagttgca
    26281 gcaattacct gtattagcca acccaagaaa acgatgttgc gtgataaagc catggtgaat
    26341 tatgcctttg attatttaag atcaccaggc agtctaccgt taaccacggc agccacggag
    26401 ctttccgcga ttcatggtca ctcaacatcg caatatcgcc ttggcgaatt ttatcttcat
    26461 ggtagcgacg gtaaaccact ggattataca caggcgagat actggtatga gcaatcagca
    26521 gaacaggaaa atccgcgcgc acaaagcaaa ctgaggtgga tctacctgaa aggcctggga
    26581 gtcaaacccg atacccgtaa agcaattctc tggtataagg aagcagctga acaagggtat
    26641 gctcatgctc aatatacttt gggtttgatc tacagaaatg gcactggtat taatgttaac
    26701 cattatgaat ctcaaaaatg gttaaaactg gccgccaaac aacattacaa aaatgcggaa
    26761 agattacttg ccggacttcc cgcacattaa cgtcgactgt cacccgaaga gatggagtat
    26821 tttccctatc aaaccggcaa agataaaaat gtagaaaata attttgatga cgcccagaca
    26881 gcccagcccg ccatcatcaa cgggcattgg ctgtggttgc ggctccggct cgacggtttc
    26941 ctgaccggta aaattcgccg cctgaattaa taatcgacga ataattaatg cttccagtgg
    27001 acaacgatct tctgcatcaa ggcggctcac aatcggcatt aatgattctt ctgtcggttg
    27061 gctggcctgc actaattgtt tggcaactaa atataatggc cataagggat gttctgtatc
    27121 acgcgtgagt gagatccgcg gcttacgtcc ttcgtgcaaa accatctcga ccagtgaacg
    27181 ccccagcgca gagagttttt catcgctgag tatttcgtat aaatgaaaac gcgtacgggc
    27241 gctgtcgccg gggacataaa gtagcggttt tccctgatcg ttaaccagat cacgcgcttc
    27301 aatcatcatc accactaatg ggtaataata gacccgatgc tgacggcacc attgcaacat
    27361 taaagcatcc gcctgggtgg agggatactc gcgccagtag taacaaagtt ctgccagcag
    27421 gctttcgcct tctccacaat agacgcgttg tgcgtattca taataacgcg catcttcatt
    27481 gtcaggttca gcttcccgcc acgaccgggc gtaatccagg agttcgggga ttcccaggcg
    27541 gcttgcgcta taccagcgga gtaaatttct gtgcagtttt gcatcatcgg ggataatcac
    27601 tgcgcgatgt tgcgttaaat acaatgtcag ccattctgga tgcgatgacc aaatccgagc
    27661 gaacatttgg taaaaatcga tggtgtcatt ttgcacggca actggcagat gcagaatggt
    27721 ctggaagtta aaaacattcc ccttcgcaat ggcatataaa aaggattcca gttcttcttc
    27781 gtctatttcc ccgacgtttt cctcctgcaa ccaccgcaat ctttctgcta ataacaccac
    27841 gcactcgaag gataaatggg cagtgttcat ggcgattgtg cacagcgacc agcgtaattc
    27901 atcaatctct tccatcgagc aataatttaa ttgctgaatg aatcgctgcc aggcggagga
    27961 cagaaaacgt tcgctatcag aggcaagtaa cgcgcgaaag gcgagcagaa tttcatgttc
    28021 tgctacctca tattcttctg gttgccaaac agatttagca ggcgactgcg caatccgtag
    28081 cgcctcctca tacgcctgac gaagttgttt aaaaccctgc ggatcggttt ctggatgaaa
    28141 ggacggtaac agcgccagat aagcgcggcg gataatatcg acgtcagtcg tttcctctat
    28201 atcgaggatc ttccagcaat ttttcataag tcgctcatat ccttaagcgt ttccgggcat
    28261 gtggggtagt cagccagtgg ttgccaggac aaggtagggt aatacgcatc atcactggca
    28321 aataattgat aaaacatatc attgccggaa tcgtcgtatt ctttacgcgc taagaattcg
    28381 cgtaataaag gcagatgatc atcttcggga gactgccaat attgccgacc aaaggtataa
    28441 ccggcaaagt attgccgcca gttactataa taacgtagag cccgtagatg aatacgcgac
    28501 tggatccata gactttcttc ttcactcaac cagttattaa gtacgcccat tcgacttaaa
    28561 aaacccatcc ggacataatc ccatgcttta attcctccct caccacagca ttcggctgtg
    28621 ctggcgacaa attgcgcata agcctgacct tgctcattta attcagcaag atagtcacgc
    28681 cattcacatg gagaataacg aaaccagcgg tgataaaacc ctgccaaccg agcggcatga
    28741 ccgttctcga tcattcggtg aattatgcgc agcaggtcat cacggtcatc aattccccat
    28801 gaggaagaaa ggtcaaaaaa gcatgtacct aaatacattt tgggggatga atgcgaaggt
    28861 aaacagcatt cattgcgtat ttccatcgga gtggaaagtg caaacaatat tttttgcgac
    28921 cccatgtcca tataatttat ccttaaacat tcatttaatg aatgtaaact acatttcgag
    28981 gctcagttat ttatactttt ctgacgcaac ataatacgca taaaataagg cttacgcaaa
    29041 aatggaatac ataaaaatat aatatatttt atctacaacc ataaggaaat gataatgatt
    29101 tttacgtcaa gttgctgcga taatttatca atagatgaga ttatcgaacg tgctgaaaaa
    29161 ggcgattgcg aggctcaata tattgttggg ttttattata atcgcgatag cgcaattgac
    29221 tctccggacg acgaaaaggc cttttactgg ctgaagctgg ccgctgagca aggtcattgt
    29281 gaagcacagt attccttagg gcaaaagtat accgaggata aaagccgtca taaagataat
    29341 gagcaagcca tcttctggct gaaaaaagct gccctacaag gccatacttt cgcttccaac
    29401 gcccttggct ggatactgga tcgtggagaa gaccccaact ataaagaagc ggttacctgg
    29461 tatcagatag ccgcggagag cggaatgtct tatgcgcaaa ataatcttgg atggatgtac
    29521 agaaatggca acggagtcgc acaagactat acgctggcat ttttctggta caaacaagct
    29581 gcattacaag gccatagtta cgcacaagac aatctggccg atctttataa agacggagaa
    29641 ggcgttgctc aaaacaagac actcgccgca ttctggtatt tgaaaagcgc acagcagggt
    29701 aatcggcacg cccagtttca aattgcatgg gattataacg ctggcgaagg ggtggaccag
    29761 gactataagc aagcgatgta ctggtatctg aaggctgccg ctcaggaaag cgtcggcgct
    29821 tacgtcaaca tcggttatat gtataaacac ggacaaggcg ttgagaaaga ttatcaggct
    29881 gcctttgaat ggtttacgaa agccgctgaa tgcaatgacg ccactgcctg gtataacctg
    29941 gccattatgt atcactacgg agaaggaaga cctgtcgatc tccgacaggc tctcgacctg
    30001 tatcgtaaag ttcagtcatc cggaaccaga gatgtcagtc aggaaattcg tgagactgaa
    30061 gctttactgt agaaataaaa acaagggccg gggaatttca ttctgcccat attctcttta
    30121 ttgctgtgcg aaatcagact attacggctc aaacgggcgt ctctggaact ggtcataacc
    30181 acatttcggg catagcgtca gcacttccgg tgtgtagatc gggagatgga agtgacattt
    30241 ctcgcagacc agatttccca gcccgaccac ttctccgctg tgataaaccc catgatgatt
    30301 gaggtcctgg aaaatttcgc gccattcaag ctgcgtttta tcggtgatgt ctgccagctc
    30361 ctgccacaag ctttctttaa tcacccgcat aaagacgctg tcagattctt ctttcaggct
    30421 ctcttcatag ctcatggcga actcttccag gtcacgtctg acagctcgcg tcagctcatc
    30481 gatctcggtt cgcgttaact cccctgtttt tattacgcgc tcgcgcgcct gttccaccag
    30541 tgcgtcgata tcacgttcgc cattgcgcag gcgttcgttc agtgacgcga ccagttcacg
    30601 gtaatattga gcaaccttgt tcatcgtttc atctcccggg tcgttaactg actataataa
    30661 tagacgttct ttgttccttg ctttgctaat acgcccacaa actctgtaaa ttaatgtacg
    30721 aataatccgg gcatcatttc aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt
    30781 gggctatgct atgcggatct gaaaaaccac ctcaacgcta catttgtagc cgtattgaaa
    30841 acaggaccac tggctgccat gcaagagcaa taccgcccgg aagagataga atccaaagta
    30901 cagcttcatt gggatgagaa gcgcacattt gaagtaaccg aagacgagag caaagagaag
    30961 tattactgcc tgtctatgct tccctatcct tctggtcgac tacacatggg ccacgtacgt
    31021 aactacacca tcggtgacgt gatcgcccgt taccagcgca tgctgggcaa aaacgttctg
    31081 cagccgatcg gctgggacgc gtttggtctg cctgcggaag gcgcggcggt gaaaaacaac
    31141 accgctccgg caccgtggac gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg
    31201 ctgggctttg gttatgactg gagccgcgag ctggcaacct gtacgccgga atactaccgt
    31261 tgggaacaga aattcttcac cgagctgtat aaaaaaggcc tggtatataa aaagacttct
    31321 gcggtcaact ggtgcccgaa cgaccagacc gtactggcga acgaacaagt tatcgacggc
    31381 tgctgctggc gctgcgatac caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa
    31441 atcactgctt acgctgacga gctgctcaac gatctggata aactggatca ctggccagac
    31501 accgttaaaa ccatgcagcg taactggatc ggtcgttccg aaggcgtgga gatcaccttc
    31561 aacgttaacg actatgacaa cacgctgacc gtttacacta cccgcccgga cacctttatg
    31621 ggttgtacct acctggcggt agctgcgggt catccgctgg cgcagaaagc ggcggaaaat
    31681 aatcctgaac tggcggcctt tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa
    31741 atggcgacga tggagaaaaa aggcgtcgat actggcttta aagctgttca cccattaacg
    31801 ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga tggagtacgg cacgggcgca
    31861 gttatggcgg ttccgggtca cgaccagcgc gactacgagt ttgcctctaa atacggcctg
    31921 aacatcaaac cggttatcct ggcggctgac ggctctgagc cagatctttc tcagcaagcc
    31981 ctgactgaaa aaggcgtgct gttcaactct ggcgagttca acggtcttga ccatgaagcg
    32041 gccttcaacg ccatcgccga taaactgact gcgatgggcg ttggcgagcg taaagtgaac
    32101 taccgcctgc gcgactgggg tgtttctcgc cagcgttact ggggcgcgcc gattccgatg
    32161 gtgacgctgg aagacggtac cgtaatgcca accccggacg accagctgcc ggtgatcctg
    32221 ccggaagatg tggtgatgga cggcattacc agcccgatta aagcagatcc ggagtgggca
    32281 aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg acactttcga cacctttatg
    32341 gagtcctcct ggtactatgc gcgctacact tgcccagagt acaaagaagg tatgctggat
    32401 tccgaagcgg ctaactactg gctgccggtg gatatctaca ttggtggtat cgaacacgcc
    32461 attatgcacc tgctctactt ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg
    32521 aactctgacg aaccagcgaa acagttgctg tgtcagggta tggtgctggc agatgccttc
    32581 tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa
    32641 cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg caggccatga actggtttat
    32701 accggcatga gcaaaatgtc caagtcgaag aacaacggta tcgacccgca ggtgatggtt
    32761 gaacgttacg gcgcggacac cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg
    32821 actctcgaat ggcaggaatc cggtgtggaa ggggctaacc gcttcctgaa acgtgtctgg
    32881 aaactggtgt acgagcacac agcaaaaggt gatgttgcgg cactgaacgt tgatgcgctg
    32941 actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa ctatcgctaa agtgaccgat
    33001 gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg cgattatgga gctgatgaac
    33061 aaactggcga aagcaccgac cgatggcgag caggatcgcg ctctgatgca ggaagcactg
    33121 caggccgttg tccgtatgct caacccgttc accccgcaca tctgcttcac gctgtggcag
    33181 gaactcaaag gcgaaggcga tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg
    33241 atggtggaag actccacgct ggtcgtggtg caggttaacg gtaaagtccg tgccaaaatc
    33301 accgttccgg tggacgcaac ggaagaacag gttcgcgaac gtgctggcca ggaacatctg
    33361 gtagcaaaat atcttgatgg cgttactgta cgtaaagtga tttacgtacc aggtaaactc
    33421 ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg atatctggca acactgttgt
    33481 tatctctggc ggtgttaatc accgccgggt gtggctggca tctgcgtgac accacgcagg
    33541 ttccttccac tatgaaggtc atgatcctgg actcaggcga tccgaacggg ccattaagcc
    33601 gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc
    33661 gtaaggacgt tccatccctg cgtttgggta aagtgagcat cgcgaaagat accgcatcgg
    33721 tattccgtaa cggtcaaaca gcagagtatc agatgatcat gacggtgaat gcgaccgtgt
    33781 tgatccccgg ccgtgatatc tacccgatta gcgccaaagt cttccgttca ttcttcgata
    33841 acccgcaaat ggcgttagcg aaagataacg aacaagacat gatcgtaaaa gagatgtacg
    33901 accgtgctgc cgaacagctg attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt
    33961 ccgacgaaga acagacgtcg accacaacgg atactccggc aacgcctgca cgcgtctcca
    34021 ccatgctggg taactgatga ttcggttgta cccggaacaa ctccgcgcgc agctcaatga
    34081 agggctgcgc gcggcgtatc ttttacttgg taacgatcct ctgttattgc aggaaagcca
    34141 ggacgctgtt cgccaggtag ctgcggcaca aggattcgaa gaacaccaca ctttttccat
    34201 tgatcccaac actgactgga atgcgatctt ttcgttatgc caggctatga gtctgtttgc
    34261 cagtcgacaa acgctattgc tgttgttacc agaaaacgga ccgaatgcgg cgatcaatga
    34321 acaacttctc acactcaccg gacttctgca tgacgacctg ctattgatcg tccgcggtaa
    34381 taaattaagc aaagcgcaag aaaatgccgc ctggtttact gctcttgcga atcgcagcgt
    34441 gcaggtgacc tgtcagacac cggagcaggc tcagcttccc cgctgggttg ctgtgcgcgc
    34501 aaaacagctc aacttagaac tggatgacgc ggcgaatcag gtgctctgct actgttatga
    34561 aggtaacctg ctggcgctgg ctcaggcact ggagcgttta tcgctgctct ggccagacgg
    34621 caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt
    34681 tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc gcgttgcata ttcttcagca
    34741 actgcgtctg gaaggcagcg agccggttat tttgttgcgc acattacaac gtgaactgtt
    34801 gttactggtt aacctgaaac gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa
    34861 gcatcgggta tggcagaacc gccggggcat gatgggcgag gcgttaaatc gcttaagtca
    34921 gccgcagtta cgtcaggccg tgcaactcct gacacgaacg gaactcaccc tcaaacaaga
    34981 ttacggtcag tcagtgtggg cagagctgga agggttatct cttctgttgt gccataaacc
    35041 cctggcggac gtatttatcg acggttgata tgaaatcttt acaggctctg tttggcggca
    35101 cctttgatcc ggtgcactat ggtcatctaa aacccgtgga aacgctggcg aatttgattg
    35161 gtctgacgcg ggtcacaatc atccctaata atgttcctcc gcatcgtccc cagccggaag
    35221 cgaacagcgt gcagcgtaaa cacatgcttg aactggcgat tgccgacaag ccattattta
    35281 ctcttgatga acgcgagcta aagcgcaatg ccccctctta cactgcgcaa acactgaaag
    35341 agtggcggca ggaacaagga ccggacgtgc cgctggcgtt tattattggt caggattcat
    35401 tgctgacctt tccgacctgg tacgaatacg aaacgatact cgacaatgca catttgatcg
    35461 tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc gcaataccag caatggctgg
    35521 aagatcattt gacacataac ccggaagatc ttcaccttca gcctgccggt aaaatttatc
    35581 tggctgaaac gccgtggttt aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg
    35641 gtgaatcgtg tgaggattta ttgccggaac cggtattgac ttacattaac caacaaggct
    35701 tgtatcgctg atacctgtcg ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg
    35761 cgaaacacaa gcgaatgtcg atggtctgta cagcggtcat gcgcccacac ccctgaccgc
    35821 acgcggtatt gagcaagcgc aaaatctgca tacactgcta gatgatgttt cctttgacct
    35881 ggttttatgc agtgaactgg aacgggcaca gcataccgcg cgactggttc tcagtgaccg
    35941 ccagctcccc gttcatatca tccctgaact caacgaaatg ttttttggcg actgggagat
    36001 gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga
    36061 ctggcagcat gctatcccca cgaatggtga aggatttcag gcattttcgc aacgtgtgga
    36121 acgctttatc gcaaggctta gtgaatatca gcactatcag aatattttaa tcgtcagcca
    36181 tcagggcgta ctgagtctgt taattgctcg tttaattggc atgcctgccg agtctatgtg
    36241 gcattttcgc gttgaccagg ggtgttggag cgccattgat atcaaccaaa aattcgcgac
    36301 gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaac gcatgacttt tctgtttttt
    36361 tacgggtaag ccgcaacgac cattgacagc cccgggcagg ctgatattct ccgcagccag
    36421 actttttccg ccagacacga ctttgtagaa attgttttac aaaaatggcg atgcaatctg
    36481 cggcgcgggg tgggatgata gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa
    36541 atcgcctctg gttcaggtat actgacagac catttttatc tatttgattc acccaggggg
    36601 aaaacttgca gggtaaagca ctccaggatt ttgttatcga caaaattgat gacctcaaag
    36661 gtcaggacat catcgcctta gacgttcaag gcaaatccag catcaccgac tgcatgatca
    36721 tctgtacggg tacgtccagc cgtcatgtta tgtccattgc tgaccacgtt gtgcaggagt
    36781 ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga aaacagcgcc gactggattg
    36841 tcgtggattt gggcgatgtg attgtccatg tcatgcagga agagagccgt cgcctgtatg
    36901 aactggaaaa actctggagt taatgcgtga agctgcaact tgtcgccgtg ggaacgaaaa
    36961 tgccggactg ggtacaaacc ggttttaccg agtacctgcg tcgttttccg aaagatatgc
    37021 ccttcgagct gattgaaatt ccggccggca aacgcggcaa gaatgcggac atcaagcgca
    37081 tactcgacaa agagggtgag cagatgttgg cggccgcagg caaaaaccgc attgtcaccc
    37141 tcgatattcc aggcaagccc tgggatacgc cgcagttagc cgctgagctg gaacgctgga
    37201 agctggatgg tcgcgacgta agtctactga ttggcgggcc tgaagggttg tcgcctgcct
    37261 gtaaagcggc ggctgagcag agctggtcgc tgtcggcgct taccctcccc catccgctgg
    37321 ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag catcaccacc aaccatcctt
    37381 atcaccgtga gtgataaggg agctttgagt agaaaacgca gcggatgaaa ctacagaact
    37441 cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt
    37501 tggggatttt gctgctgacc ggcgtgctta tcgccaacct gtataatctg caaattgttc
    37561 gctttaccga ctaccagacc cgctctaatg aaaaccgcat taagctggtg cctatcgcgc
    37621 ccagccgcgg cattatctac gaccgtaacg gtatccctct ggccctcaac cgcactatct
    37681 accagataga aatgatgccg gagaaagtcg ataacgtgca gcaaacgctg gacgctttgc
    37741 gcagcgtggt agatctgacc gatgacgata ttgctgcatt ccgaaaagag cgcgcacgtt
    37801 cacaccgttt cacctctatt ccggtgaaaa ctaacctaac cgaagtacaa gtagctcgct
    37861 ttgccgtcaa tcagtaccgt tttccgggtg tcgaagttaa aggctataaa cgtcgttact
    37921 atccttacgg ttcggcgttg acccacgtca tcggctatgt gtcgaaaatc aacgataaag
    37981 acgtcgaacg cctgaataat gacggcaaac tggccaacta tgcggcaacg catgatattg
    38041 gtaagctggg cattgagcgt tactatgaag acgtgctgca cggtcagacc ggttatgaag
    38101 aggttgaagt taacaaccgt gggcgtgtta tccgccagtt aaaagaagta ccaccgcaag
    38161 ccggacacga tatttacctg acgctggatc tcaaactcca gcaatatatt gaaacgctgc
    38221 tggcgggtag ccgcgcagct gtggttgtta ccgaaccgcg tacaggtggg gtgctggcgc
    38281 tggtttccac gcctagttat gacccaaacc tgtttgttga cggtatctcc agcaaagatt
    38341 attccgcctt gctgaacgac ccgaatacac cgctggtgaa ccgcgccaca cagggggttt
    38401 atcctcccgc gtctacagtt aaaccctatg tggcggtttc ggcattaagc gccggggtga
    38461 tcacgcgcaa tacgacgctg tttgacccag gctggtggca actaccaggg tcggaaaaac
    38521 gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa tgtcacaaga tcgctggaag
    38581 aatctgcgga taccttcttc tatcaggtgg cctacgatat ggggatcgat cgcctctccg
    38641 aatggatggg taaattcggt tatggtcatt acaccggtat cgacctggcg gaagaacgtt
    38701 ccggcaacat gcctacccgc gaatggaaac agaaacgctt taaaaaaccg tggtatcagg
    38761 gtgacaccat tccggttggt atcggccagg gttactggac agcgacccca atccagatga
    38821 gtaaggcact gatgatcctg attaatgacg gtatcgtgaa ggttccgcat ttgctgatga
    38881 gcaccgccga agacggcaaa caggtgccat gggtacaacc gcatgaaccg cccgtcggcg
    38941 atattcattc cggttactgg gagctggcga aagacggtat gtacggtgtt gctaaccgcc
    39001 ctaatggtac ggcgaataaa tactttgcca gcgcaccgta caaaattgcg gcgaaatctg
    39061 gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta taatgcgcac aaaattgccg
    39121 agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc atacaacaat ccgcaagtgg
    39181 ctgtcgccat gattctggag aacggcggcg cgggtccggc ggttggtacg ctgatgcgcc
    39241 agatcctcga ccacattatg ctgggtgata acaacaccga tctgcctgcg gaaaatccag
    39301 cggttgccgc agcggaggac cattaatcat gacggataat ccgaataaaa aaacattctg
    39361 ggataaagtc catctcgatc ccacaatgct gctgatttta ctggcattgc tggtttacag
    39421 cgccctggtt atctggagcg ccagcggtca ggatattggc atgatggagc gtaaaatcgg
    39481 ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg caaattcctc cacgcgttta
    39541 tgaaggctgg gccccctatc tctatatcat ctgtattatt ttgctggtgg cggtagatgc
    39601 tttcggagcc atctctaaag gcgctcaacg ctggctggac ctcggtattg ttcgttttca
    39661 gccgtcggaa attgccaaaa tagccgtacc actgatggtt gcgcgcttta tcaaccgcga
    39721 cgtttgcccg ccatcgttga agaatactgg catcgcgctg gtgctgatat ttatgcccac
    39781 gctgctggtg gctgcacagc ctgacctggg aacatcaatc ctcgttgcgc tttccggtct
    39841 gtttgtactg ttcctctctg gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt
    39901 agcggcgttc attccgattc tgtggttctt cctgatgcat gattaccagc gccagcgcgt
    39961 aatgatgctc ctggacccgg aatcagaccc actcggcgcg ggctatcaca ttattcagtc
    40021 taaaattgct attggctccg gcggattacg cggcaaaggc tggctgcacg gcactcagtc
    40081 acagcttgaa tttctccccg aacgccatac tgactttatc ttcgcggtac tggcggaaga
    40141 gctgggatta gtgggcattc tgattctgct cgctctctac attctgctga tcatgcgcgg
    40201 gctgtggata gccgccagag cgcaaaccac ctttggtcgc gtcatggctg gtggcttaat
    40261 gctgatatta ttcgtttatg tcttcgtaaa tattggtatg gtaagcggta ttctgccggt
    40321 tgtaggggtt ccgctcccac tggtcagtta tggaggatcg gcgctaattg tgctgatggc
    40381 tgggttcggg attgtaatgt caatccacac ccacaggaaa atgttgtcga aaagcgtgta
    40441 agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat cgcggcagga atgctcgcgg
    40501 catgtacaag cgatgatggt cagcaacaga cggtaagtgt accgcagcct gcggtatgta
    40561 acggccccat agttgaaatt agcggggcgg acccgcgttt cgaaccactg aacgcgacgg
    40621 caaatcagga ttaccagcgc gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat
    40681 ttagccaggc gggactggcg gcaatctatg atgccgaacc aggcagtaac ctgacggcct
    40741 ctggcgaagc tttcgatccg acaaagctga cggcggccca tccaacgctt ccgatcccca
    40801 gctacgccag aatcactaac ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc
    40861 gcggccctta cggcaacgac cgcgttattt cgctttctcg cgcggcagct gaccgtctta
    40921 acacgtcaaa caacaccaaa gttcgtatcg atccgattat tgtcgcccag gatggttcgc
    40981 tttctggtcc tggtatggct tgtaccacag tcgccaaaca gacttacgcc ctgcctgcac
    41041 ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc tggcccgcag ggtgacattc
    41101 ttccggtcag taattcgacg ctaaaaagcg aagatccgac cggcgcgccg gtaaccagta
    41161 gcggtttcct cggcgctcca acgaccttag cgcctagtgt actggaaggc agcgaaccga
    41221 cgcctgcgcc acagcccgtt gttacagctt cgtcgacaac gcctgcaacc tcgcctgcaa
    41281 tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa ctttatggtg caagtcgggg
    41341 ccgtaagcga tcaggctcgt gcgcaacagt accaacagca actgggacag aagttcggcg
    41401 tccccggtcg cgtaactcaa aatggcgcgg tctggcggat ccagcttggc ccattcgcca
    41461 gcaaagccga agccagtacc ttgcagcaac gtttgcaaac cgaagcccaa ttacagtcat
    41521 ttattactac cgcgcagtag cgtaaagcag gcatctgatg tgtcaatttc tgtaaatgac
    41581 gttaacaaag tcacgcggaa agtcagatgc ctgccggtag tgcatttgct atagtagggc
    41641 acttttttaa ttccatcacg gatgtcgtag ttcagaccat gaataccatt ttttccgctc
    41701 gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac agcctttatc tctgctgcac
    41761 atgccgatga cctgaatatc aaaactatga tcccgggtgt accgcagatc gatgcggagt
    41821 cctacatcct gattgactat aactccggca aagtgctcgc cgaacagaac gcagatgtcc
    41881 gccgcgatcc tgccagcctg accaaaatga tgaccagtta cgttatcggc caggcaatga
    41941 aagccggtaa atttaaagaa actgatttag tcactatcgg caacgacgca tgggccaccg
    42001 gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa accgggcatg caggttccgg
    42061 tttctcagct gatccgcggt attaacctgc aatcgggtaa cgatgcttgt gtcgccatgg
    42121 ccgattttgc cgctggtagc caggacgctt ttgttggctt gatgaacagc tacgttaacg
    42181 cactgggcct gaaaaatacc cacttccaga cggtacatgg tctggatgct gatggtcagt
    42241 acagctccgc gcgagatatg gcgctgatcg gccaggcatt gatccgtgac gtaccgaatg
    42301 aatactcgat ctataaagaa aaagaattta cgtttaacgg tattcgccag ctgaaccgta
    42361 acggcctgtt atgggataac agcctgaatg tcgacggcat caaaaccgga cacactgaca
    42421 aagcaggtta caatcttgtt gcttctgcga ctgaaggcca gatgcgcttg atttctgcgg
    42481 tgatgggtgg acgtactttt aaaggccgtg aagccgaaag taaaaaactg ctaacctggg
    42541 gcttccgttt cttcgaaacc gttaacccac tgaaagtagg taaagagttc gcctctgaac
    42601 cggtttggtt tagtgattct gatcgcgctt cgttaggggt tgataaagac gtgtacctga
    42661 ccattccgcg tggtcgcatg aaagatctga aagccagcta tgtgctgaac agcagtgaat
    42721 tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat caacttccag cttgatggca
    42781 aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg
    42841 gcaaaatcat tgattacatt aaattaatgt tccatcactg gtttggttaa aaattgaaca
    42901 cttgaaagtg taatttccgt ccccatatac taagcatcag taaaaaaact cccgccttct
    42961 ggcgggagtt gctatttaat tacgttacgc cggagctgac atgaaaacca aacttaacga
    43021 actgcttgaa ttccctactc cttttactta caaagttatg gggcaggcgt tacctgagct
    43081 ggttgatcag gtggttgaag tggtacagcg ccatgcgcca ggtgactaca ccccaacggt
    43141 aaaaccaagc agcaaaggca actaccactc ggtatctatc actatcaacg ccactcatat
    43201 cgagcaggtt gaaacactgt atgaagaact gggcaaaatc gatattgtcc gcatggttct
    43261 gtaactcgct tctccgttac ccggctcctg gtcgggtaac tcccctctcc tcgctgtgat
    43321 atacttttcc cccactttta ctcattctcc acggagatgc cgttttgtat caggataaaa
    43381 ttcttgtccg ccagctcggt cttcagcctt acgagccaat ctcccgggct atgcatgaat
    43441 tcaccgatac ccgcgataat agtacccttg atgaaatctg gctggtcgag cactatccgg
    43501 tattcaccca aggtcaggca ggaaaagcgg agcacatttt aatgccgggt gatattccgg
    43561 tgatccagag cgatcgcggt gggcaggtga cttatcacgg gccgggacaa caggtgatgt
    43621 atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg
    43681 agcaaacagt ggtgaatacc ctggctgaac tgggtataga agcgcatcct cgggctgacg
    43741 cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact gggtttacga attcgacgcg
    43801 gttgttcatt ccacggtctg gcattaaacg tcaatatgga tctttcacca tttttacgta
    43861 ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg aaacccgaag
    43921 cgacgactaa taatattgct ccacgtttac tggaaaatat tttagcgcta ctaaacaatc
    43981 cggacttcga atatattacc gcttaattcc atacatcaat ggcccaattt acactgggtc
    44041 attactcata atacctgcta atgctttaca tctccttcgc gtttgtatat tttctttatc
    44101 acttgcagaa atgcggtgcc acgccttgct ccgtaactca ccccattgat taacctattg
    44161 aaatagttga ttatggtgag caaaagcgtt ccgttccaat tagacataag attattgtaa
    44221 ttttttaaat attattttcc catgaataaa attggagtaa gtgcgtggat agtaataatc
    44281 aaattgaacc ctgtttaagc agaaagtcgt cagaaggtaa accacaaata tttacaacct
    44341 tacgaaatat tgatcttaac cttctgacta tttttgaagc tgtatatgta cataaaggga
    44401 tcgttaatgc agcgaaagtg cttaatctga ccccctcggc aatcagtcag tctattcaga
    44461 aactgcgcgt tatattccct gacccattgt ttattcgcaa aggccagggt gtcactccta
    44521 ccgcatttgc gatgcatcta catgagtata tcagtcaggg ccttgagtcc attcttggcg
    44581 cgctggatat cgaaggaagc tatgataagc aacgaacgat aactattgcg accactccct
    44641 cggtcggagc cctggtcctt cctgtcatct atcgggcgat taaaactcac tatccgcagc
    44701 ttttactgcg caacccgccc atcagcgacg cggaaaacca actcagtcag tttcaaaccg
    44761 atctcatcat cgataacatg ttttgcacca atcgtacggt gcaacatcat gttctgttca
    44821 ccgacaatat ggtgttaatt tgccgcaagg gaaatccact actctcttta gaagatgaca
    44881 gagagactat cgacaacgct gcgcatgtac ttctgttacc ggaagggcaa aatttcagcg
    44941 gtctgcggca gagagttcaa gagatgtttc cggaccggca aatcaatttc accagctaca
    45001 acattttgac aatcgctgca ctggttgcca acagtgacat gttagcgatt attcccagcc
    45061 gtttttataa cctgtttagt cgttgctggc cgctggaaaa actgcctttt ccgtccttaa
    45121 atgaggagca aatagatttc tctatccact acaacaaatt catcctgcgt gatcccatcc
    45181 tgcacggagt aatagatgtt atccgtaatg cattttgaaa aagtattgtt acagccagat
    45241 cacagcgcag aacgataaaa tggcacaatc caacaacaat tttacatttt cgcgaccgct
    45301 ttggctgctt tcaggtccgt ttcaatgata tactgccagt cgttaattca aaaatagttg
    45361 ataattacaa caatctattg aattgaaacg ctttccttcg taattcgcaa ctggaacacg
    45421 cacgctatga gtaaacccat tgtgatggaa cgcggtgtta aataccgcga tgccgataag
    45481 atggccctta tcccggttaa aaacgtggca acagagcgcg aagccctgct gcgcaagccg
    45541 gaatggatga aaatcaagct tccagcggac tctacacgta tccagggcat caaagccgca
    45601 atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct cctgccctaa cctggcggaa
    45661 tgcttcaacc acggcacagc aacgtttatg atcctcggcg ctatttgtac ccgccgttgt
    45721 ccgttctgtg atgttgccca cggtcgcccg gtagctcctg atgccaatga accagtgaaa
    45781 ctggcgcaga ccattgccga tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt
    45841 gatgacctgc gcgatggcgg tgcccaacac tttgcggatt gtattactgc cattcgggaa
    45901 aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt tccgcggtcg aatggatcgt
    45961 gctctggata ttctgactgc aacgccccca gatgtgttca accataacct ggaaaacgta
    46021 ccgcgtattt accggcaggt acggcctggc gcagattaca actggtcgct gaagctgctg
    46081 gaacgcttta aagaagcgca tccggaaatc ccgaccaagt ctggtctgat ggtgggtctg
    46141 ggtgaaacca atgaagaaat tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg
    46201 atgttaacgc tggggcaata tttgcagcca agccgccatc acctgccggt tcaacgttac
    46261 gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat
    46321 gctgcatgcg gtccgtttgt ccgctcttct taccacgccg atttgcaggc gaaaggaatg
    46381 gaagttaagt aatcctgtaa taattactta catctgtctg ataaaaaacc cgctttttga
    46441 agcgggtttt ttgtatcgaa caagatattg agggagcgtc ctgctcgcca cgtcactctt
    46501 tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcgt
    46561 catcattcat cgccttcttg aaccctttaa tggccgctcc aaggtctccg cccagcgtac
    46621 gtaacttctt agtcccaaac agcagaacga ccagcgccgc aactaccagc agtttggtaa
    46681 tactaatctc acccatagat accttcttga catataaccg gcaacagaac gcgccttaaa
    46741 aaccagagaa tattaacggt cattcgacgc gcgcacacaa tagcaatctg taacaaggtg
    46801 aatcaagcgc cgagtaaaaa aacatcataa taattgcggc ggcgcaaagc gacggttgtt
    46861 taagatgggc agttgcgcgc gcacctgacg cacacgttcg ggcgtcactt ccgccataat
    46921 gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca aagggatcta taatccggct
    46981 ttgcccgata tttttgttcc cgcactcccc cgccgccacc atataacagg tggtatccag
    47041 cgcacgagcg gcaagcaacg ttgaccagtg atgctctttg agcggcccac gaacccaggc
    47101 ggcaggaagt accaggattt cagctccctg taaagcctgt gccagcgcca gctctggaaa
    47161 gcgtaagtca taacaggtca tcagaccgac cttcatccct tccacctcca gtaacggagc
    47221 tatttcatta ccagcatcaa catggcgtga ttcctgaatg gcaaatgcat catagagatg
    47281 cagtttggca taacgggcga cgatgtttcc tgcctgaagt gccaccagca tattccatgc
    47341 gcgccccggc gttgaaggaa gatgaatcgt cagaattgtc gtcatcatgt tacgtttact
    47401 ttctcgccgt aaacgtccga ggaattcgcc ttccagcagc tgtgctgatt taaccgatag
    47461 atctgcatca tgatcatcgc gcgccagcaa tgcttccggc aggacaaaca gcgatacgtc
    47521 gttttccgcc gcctgcgcca tcaacgaggc acaaatctca gcgttctttt cccacacaga
    47581 tgtaacagca aactgtcctg cagcaaccag cattgccacc tcctgtcaga gtcgtaaaat
    47641 tcgccaacga cgattattcg gcgttacact tatcactcat acaaatcaaa tagcaggatt
    47701 ttgcagtgtt acaacttctt ttagcagttt ttattggcgg tggtacggga agcgtagcga
    47761 gatggctgtt aagtatgcga tttaacccgc tgcatcaggc gattccgttg gggacgctgg
    47821 cagcaaacct gattggggca ttcatcatag gaatgggatt cgcctggttc agcaggatga
    47881 cgaacattga tccagtgtgg aaagtattaa tcaccaccgg attttgtggc ggtctaacaa
    47941 ccttctcaac attttcggca gaagtggtgt ttttgttaca agagggccgc tttggctggg
    48001 cattactgaa cgttttcgtc aaccttctgg ggtcttttgc catgaccgca ctggcattct
    48061 ggctgttttc ggcctcaacc gtacactaaa gaccaaaaaa aacccgctga ttaagcgggt
    48121 tttgaattct tgctgacgta tcttacagag cgattacgtt tgcagcagaa gggcctttgg
    48181 caccgttagt gatttcgaac tctacgcgct gaccttcagc aagagtttta aaaccattag
    48241 tctggattgc agagaagtgt acgaacacgt ctttgctgcc gtcttccgga gtaatgaaac
    48301 cgaatccttt ggactcatta aaccacttaa cgttaccttt aatcttagac atcaaaatta
    48361 cctttacatg aaaaatagac acaaatgctg tgtcggttat cagtacacca attgtggtac
    48421 gctttgtcca gtgcaacttt actaaaaagt gataaaagtc gcgttatttt tgatgttgct
    48481 tattttagtt attgatttta aagaagttac taaaacttca tttgtctcaa aactgaaagc
    48541 gcatccaggc aaagtacaca ttgccattgt tgtaggtacc cggaatgtag gtcatctgaa
    48601 aagtcactgg gccataaccc acggaggcca atggcagtag aaccgggaga gggatgtaat
    48661 tccagttatc gcgtgccgtt acgccagcgg tgaatcccag acctaaatga aaattttcat
    48721 ccgccagcgg tcgccaggta ctttcccatc cgtatccggc aatcggttcc catttgttcc
    48781 acgagtcctt aaatgccatg gcatacaggc catgccagtt tcctttttca tcccaacgcg
    48841 acaggccaaa accgccaccc cacggtcgct cgttatagcg atcggttttt tctttgtcgt
    48901 aagcgaaacg tgcatgccag gtgatggcag gaatatataa atcataatgt tcaggctgtt
    48961 gccaggtttg tgcaatattt tctctaaacg ttgtcatcct ctcatctgcg ttagcaaaaa
    49021 ctttaccaac gctgattaac tgaataaaaa caaaggaaaa gatagcgaca tatttactca
    49081 cgttcatttg tgaccataaa acatttatca aaaatctact actagcatag caaagctaca
    49141 attaacataa ccttaataga cccaacataa agaataatct gaatagctgc tctatttatt
    49201 tccgcttgtt caagttaaat atttccgcgc atcgattaaa gatgatgcgt cgttttctgc
    49261 gatgggaata gtcaaaaaag aaaaaccaag tcttttttga tgacaaaatg caatcaagga
    49321 aaaattattt tattttttta acgagataca acaatcatct taacgaagta tataatatat
    49381 atcgtcaacc atgctcatat tagattattt tttgagcgca cagccgcagc taacaccatt
    49441 gcaattaaca aatttgcatc aatccaccat caatttacac acattattat gtgataattg
    49501 ccaaccgcta aatatgcgtt ttgttatcta tgtataaaaa cagcaacttc aatgtcttaa
    49561 tggcagtttt tcttgatttt aatcagcatt catcgccaat ttattgggca tattttttcc
    49621 ttaagcttta ggaagttttt attatttact ttgggcctgg agacaggaaa aattatgctg
    49681 acattcattg aactccttat tggggttgtg gttattgtgg gtgtggctcg ctacatcatt
    49741 aaagggtatt ccgccactgg tgtgttattt gtcggtggcc tgttattgct gattatcagt
    49801 gccattatgg ggcacaaagt gttaccgtcc agccaggctt caacaggcta cagcgccacg
    49861 gatatcgttg aatacgttaa aatattacta atgagccgcg gcggcgacct cggcatgatg
    49921 attatgatgc tgtgtggctt tgccgcttac atgacccata tcggcgcgaa tgatatggtg
    49981 gtcaagctgg cgtcaaaacc attgcagtat attaactccc cttacctgct gatgattgcc
    50041 gcctattttg ttgcttgtct gatgtcactg gccgtctctt ccgcaaccgg tctgggtgtt
    50101 ttgctgatgg caaccctgtt tccggtgatg gtaaacgttg gtatcagtcg tggcgcagca
    50161 gctgccattt gtgcctcccc ggcggcgatt attctcgcac cgacttcagg ggatgtggtg
    50221 ctggcggcgc aggcttccga aatgtcgctg attgacttcg ccttcaaaac gacgctgcct
    50281 atctcaattg ctgcaattat cggcatggcg atcgcccact tcttctggca acgttatctg
    50341 gataaaaaag agcacatctc tcatgaaatg ttagatgtca gtgaaatcac caccactgcc
    50401 cccgcgtttt atgccatttt gccgttcacg ccgatcatcg gtgtactgat ttttgacggt
    50461 aaatggggtc cgcaattaca catcatcact attctggtga tttgtatgct gattgcctcc
    50521 attctggagt tcctccgcag ctttaatacc cagaaagttt tctctggtct ggaagtggct
    50581 tatcgcggga tggcagatgc gtttgctaac gtggtgatgc tgctggttgc cgctggggta
    50641 ttcgctcagg ggcttagcac catcggcttt attcaaagtc tgatttctat cgctacctcg
    50701 tttggttcgg cgagtatcat cctgatgctg gtattggtga ttctgaccat gctggcggca
    50761 gtcacgaccg gttcaggcaa tgcgccgttt tatgcgtttg ttgagatgat cccgaaactg
    50821 gcgcactctt ccggcattaa cccggcgtat ttgactatcc cgatgctgca ggcgtcaaac
    50881 ctggatcgta ccctatcacc cgtttctggc gtagtcgttg cggttgccgg gatggcgaaa
    50941 atctcaccat ttgaagtcgt aaaacgcacc tcggtgccgg tgcttgttgg gctagtgatt
    51001 gttatcgttg ctacagagct gatggtgcca ggaacggcag cagcggtcac agacaagtaa
    51061 atagtaatgc cggaggcaag tttcctccgg ctttatcatg tcacccactg tggtatatgc
    51121 gttgtggtct gccaactttg ccgtgaacaa tttcagcaat aatcagatgg cggctggcgc
    51181 aatattcaag ataacgcctg gcagtggtgc ggctgatggt cagtgcctgc gccaccgttt
    51241 ctgccgtatg ttgcacacca ggctctttaa acagttttcg caccgcgttt agcgtcaagg
    51301 ggtcaatgcc ggtcggcagt tcgtctttgg gttcaccgcg agcataggca ttaaacatct
    51361 catcaatttg cttctggctg gcgctatcaa tactttccag catatgttta cgctggcgga
    51421 aacgggttag cgtttgcccc agtgtattga ttcacttgaa gtacgaaaaa accgggagaa
    51481 cattggatta ttcgggatct gatgggatta gatttggtgg ggcttgcaag cctgtagtac
    51541 aaattttagg cgttaaccaa tgaaacgcga aagatagtaa aaaattgctt ttgtttcatt
    51601 gaaaaaacga aaaacaaaaa cactataaat catttcaatc atagcttcaa aaaacgttca
    51661 aaaccgataa caaccaggct gtcaccaaat gactcatatc acaaatcagc tcatgccgtt
    51721 taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggc tcatggttgt
    51781 tatacggctt ttttcgcatc ttgtggttcc aacaaaggag ctacaggctc ttcctgttta
    51841 tgaacaccat acattgacaa aatctcttgg cgagtttctg gaggtaattt atgcagcgcc
    51901 aaagctgttt gtagtgtttg gcgatcaata cccagcgttg ccaatagggt gtcaacggag
    51961 atggaggggc gttcttgcat taatccggaa agcccatatt tagccatgtt tagctctatc
    52021 tctcggactt cattttctcc tagtttattg attaacaggg agaacatttt caatgtatca
    52081 gcattcattt cttcgatagc aattttttgc atagccggta ttgccgggat atattcaacc
    52141 ccaggcccgc gacgcccttc ttgtgaacgc ttcggccatc cttctttctt tgctcgttta
    52201 tgcaccccct gtattgtccc aggcattcca tccagttcag taatttgttt gggtgtcatc
    52261 cactggttta tctcatttgc gcttttcata aactaagttt accagattta tctatataaa
    52321 aatcaatgga ttacaaaata aaccacatta actgatataa accggttttg tttatttggt
    52381 aatgtataaa acagataatc aagacgaatt atttgttttg gtaacccttt atcatacgca
    52441 atatggttgg tgtgcgaaag atgagtaacg attggcatcg ggccgacgtg attgcggccc
    52501 tcaaaaaaac aggaacccct ctggcaaaat tgtccagacg ttcaggttat tcaaccggga
    52561 cgcttgcaaa tgcattagtc aggccatggc ctaaaggtga acgcattatt gctgacgcct
    52621 taaacatgaa acctgaggaa atttggccta gtagatatct aagggatcac aatgagagag
    52681 tggatatcag caattgaatg catgggttta tctggtttac ctggttcatt aatgggggtt
    52741 cacaaaagag ctcagagaga aggatggctc aagagaagaa gagaaggaat tagaggggtt
    52801 gcttacgaat accatatatc ttctcttcct aacattgctc gagctgaagt tttgctccaa
    52861 caaggaaaat ttaaaaccag tcagggatgt tttgaaatcg cccgccccac gctggaagct
    52921 catgattatg atcgtgaggc actatggagc aaatgggata aagccagcga ttcccagcgc
    52981 agcctggctg aaaaatggtt gccctcaatt caggccacag acgaaatgct gaaccagggg
    53041 atttcaacga aaacggcttt tgcgaccgtt gcagggcatt accaggtcag cgcatccact
    53101 ttgcgggaca agtattacca ggtacagaag tttgcgaagc ctgactgggc ggctgcactt
    53161 gttgatggac gtggagcatc ccgtcgcaat gttcacaaaa gtgaatttga cgaggatgcc
    53221 tggcagtttc tgattgcaga ttatctgcga ccggaaaaac ccgccttccg caaatgttat
    53281 gagcgtctgg aactggcagc ccgcgagcat ggctggagta ttccctcccg tgccacggcc
    53341 tttcgccgga ttcagcaact ggacgaggca atggttgttg cctgtcgtga aggtgaacat
    53401 gcactgatgc atctgatacc ggcacagcag cgaactgtgg aacacctgga cgccatgcag
    53461 tggatcaacg gcgacggtta tctgcataac gtctttgtac gctggtttaa cggtgatgtg
    53521 atccgtccga aaacatggtt ctggcaggat gtgaaaaccc gaaaaattct gggctggcgc
    53581 tgcgatgtga gcgagaacat tgattcaatt cgcctctcgt tcatggatgt tgtgactcgc
    53641 tacagtatcc cggaggattt tcacatcacc attgataaca cccgtggtgc tgcgaataaa
    53701 tggctgacgg gaggcgcgcc caatcgctac cgctttaagg taaaagagga cgatccaaaa
    53761 ggactgtttt tactgatggg tgcgaaaatg cactggacaa gcgttgttgc cggtaaaggc
    53821 tggggccagg caaaacctgt tgaacgtgct ttcggtgttg gtgggcttga ggaatacgtt
    53881 gataagcatc cggcactggc tggcgcatat acggggccaa atccgcaggc aaaacctgat
    53941 aactatggcg accacgctgt tgatgcagag ctgtttctga aaacctttgc cgaaggtgtg
    54001 gcgatgttca atgccagaac aggccgtgaa acagaaatgt gcgggggcaa actctcgttt
    54061 gatgatgttt tcgagcgtga atacgccaga acgattgtgc gtaagccaac cgaagaacaa
    54121 aaacggatgc tgttactgcc tgccgaggcg gtgaacgttt cacgcaaagg cgagtttacg
    54181 cttaaagttg gcggctccct taaaggcgcg aaaaacgttt attacaacat ggcattaatg
    54241 aatgccggag tgaaaaaagt tgtggtcaga tttgatccac agcagttaca cagcacggtt
    54301 tattgag
//
LOCUS       DAJVRP010000015        53652 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVRP010000015 DAJVRP010000000
VERSION     DAJVRP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.015"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..209)
                     /locus_tag="OQN12_000884"
     CDS             complement(<1..209)
                     /locus_tag="OQN12_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653320.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDR"
     gene            complement(211..431)
                     /locus_tag="OQN12_000885"
                     /pseudo
     CDS             complement(211..431)
                     /locus_tag="OQN12_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(424..645)
                     /locus_tag="OQN12_000886"
     CDS             complement(424..645)
                     /locus_tag="OQN12_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653321.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(635..835)
                     /locus_tag="OQN12_000887"
     CDS             complement(635..835)
                     /locus_tag="OQN12_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1653322.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            965..1144
                     /locus_tag="OQN12_000888"
     CDS             965..1144
                     /locus_tag="OQN12_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653323.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1269..1598
                     /locus_tag="OQN12_000889"
     CDS             1269..1598
                     /locus_tag="OQN12_000889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653324.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1648..1836)
                     /locus_tag="OQN12_000890"
     CDS             complement(1648..1836)
                     /locus_tag="OQN12_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1653325.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2211..3439)
                     /locus_tag="OQN12_000891"
                     /pseudo
     CDS             complement(2211..3439)
                     /locus_tag="OQN12_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3688..4809
                     /gene="roxA"
                     /locus_tag="OQN12_000892"
                     /gene_synonym="ycfD"
     CDS             3688..4809
                     /gene="roxA"
                     /locus_tag="OQN12_000892"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1653326.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4858..6084)
                     /gene="pepT"
                     /locus_tag="OQN12_000893"
     CDS             complement(4858..6084)
                     /gene="pepT"
                     /locus_tag="OQN12_000893"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1653327.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6334..7470
                     /gene="potA"
                     /locus_tag="OQN12_000894"
     CDS             6334..7470
                     /gene="potA"
                     /locus_tag="OQN12_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1653328.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7454..8311
                     /gene="potB"
                     /locus_tag="OQN12_000895"
     CDS             7454..8311
                     /gene="potB"
                     /locus_tag="OQN12_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1653329.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8308..9102
                     /gene="potC"
                     /locus_tag="OQN12_000896"
     CDS             8308..9102
                     /gene="potC"
                     /locus_tag="OQN12_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1653330.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            9099..10145
                     /gene="potD"
                     /locus_tag="OQN12_000897"
     CDS             9099..10145
                     /gene="potD"
                     /locus_tag="OQN12_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1653331.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10301..11122)
                     /gene="cobB"
                     /locus_tag="OQN12_000898"
     CDS             complement(10301..11122)
                     /gene="cobB"
                     /locus_tag="OQN12_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1653332.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11138..12049)
                     /gene="nagK"
                     /locus_tag="OQN12_000899"
     CDS             complement(11138..12049)
                     /gene="nagK"
                     /locus_tag="OQN12_000899"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1653333.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(12078..13322)
                     /gene="lolE"
                     /locus_tag="OQN12_000900"
     CDS             complement(12078..13322)
                     /gene="lolE"
                     /locus_tag="OQN12_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1653334.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13322..14023)
                     /gene="lolD"
                     /locus_tag="OQN12_000901"
     CDS             complement(13322..14023)
                     /gene="lolD"
                     /locus_tag="OQN12_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1653335.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(14016..15215)
                     /gene="lolC"
                     /locus_tag="OQN12_000902"
     CDS             complement(14016..15215)
                     /gene="lolC"
                     /locus_tag="OQN12_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1653336.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15477..16550
                     /locus_tag="OQN12_000903"
     CDS             15477..16550
                     /locus_tag="OQN12_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1653337.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16678..20124
                     /gene="mfd"
                     /locus_tag="OQN12_000904"
     CDS             16678..20124
                     /gene="mfd"
                     /locus_tag="OQN12_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1653338.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20267..21229
                     /gene="ldtC"
                     /locus_tag="OQN12_000905"
     CDS             20267..21229
                     /gene="ldtC"
                     /locus_tag="OQN12_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCS1653339.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21312..21524)
                     /locus_tag="OQN12_000906"
     CDS             complement(21312..21524)
                     /locus_tag="OQN12_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1653340.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21809..22441
                     /gene="comR"
                     /locus_tag="OQN12_000907"
     CDS             21809..22441
                     /gene="comR"
                     /locus_tag="OQN12_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1653341.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22503..23042)
                     /locus_tag="OQN12_000908"
     CDS             complement(22503..23042)
                     /locus_tag="OQN12_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1653342.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23269..24573)
                     /gene="ndh"
                     /locus_tag="OQN12_000909"
     CDS             complement(23269..24573)
                     /gene="ndh"
                     /locus_tag="OQN12_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1653343.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24981..25523)
                     /locus_tag="OQN12_000910"
     CDS             complement(24981..25523)
                     /locus_tag="OQN12_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1653344.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25546..26571)
                     /gene="nagZ"
                     /locus_tag="OQN12_000911"
     CDS             complement(25546..26571)
                     /gene="nagZ"
                     /locus_tag="OQN12_000911"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1653345.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26582..27406)
                     /gene="thiK"
                     /locus_tag="OQN12_000912"
     CDS             complement(26582..27406)
                     /gene="thiK"
                     /locus_tag="OQN12_000912"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1653346.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27387..28028)
                     /gene="lpoB"
                     /locus_tag="OQN12_000913"
     CDS             complement(27387..28028)
                     /gene="lpoB"
                     /locus_tag="OQN12_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1653347.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(28043..28420)
                     /locus_tag="OQN12_000914"
     CDS             complement(28043..28420)
                     /locus_tag="OQN12_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1653348.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28423..28782)
                     /gene="hinT"
                     /locus_tag="OQN12_000915"
     CDS             complement(28423..28782)
                     /gene="hinT"
                     /locus_tag="OQN12_000915"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1653349.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            28913..29094
                     /locus_tag="OQN12_000916"
                     /pseudo
     CDS             28913..29094
                     /locus_tag="OQN12_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29127..31312
                     /gene="fhuE"
                     /locus_tag="OQN12_000917"
                     /pseudo
     CDS             29127..31312
                     /gene="fhuE"
                     /locus_tag="OQN12_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31372..32805)
                     /gene="ptsG"
                     /locus_tag="OQN12_000918"
     CDS             complement(31372..32805)
                     /gene="ptsG"
                     /locus_tag="OQN12_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1653350.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(33100..33897)
                     /locus_tag="OQN12_000919"
     CDS             complement(33100..33897)
                     /locus_tag="OQN12_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1653351.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33908..34912)
                     /gene="holB"
                     /locus_tag="OQN12_000920"
     CDS             complement(33908..34912)
                     /gene="holB"
                     /locus_tag="OQN12_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1653352.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34909..35550)
                     /locus_tag="OQN12_000921"
     CDS             complement(34909..35550)
                     /locus_tag="OQN12_000921"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1653353.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35540..36562)
                     /gene="yceG"
                     /locus_tag="OQN12_000922"
     CDS             complement(35540..36562)
                     /gene="yceG"
                     /locus_tag="OQN12_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1653354.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36565..37374)
                     /gene="pabC"
                     /locus_tag="OQN12_000923"
     CDS             complement(36565..37374)
                     /gene="pabC"
                     /locus_tag="OQN12_000923"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1653355.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37494..38735)
                     /gene="fabF"
                     /locus_tag="OQN12_000924"
     CDS             complement(37494..38735)
                     /gene="fabF"
                     /locus_tag="OQN12_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1653356.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38822..39058)
                     /gene="acpP"
                     /locus_tag="OQN12_000925"
     CDS             complement(38822..39058)
                     /gene="acpP"
                     /locus_tag="OQN12_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1653357.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39269..40003)
                     /gene="fabG"
                     /locus_tag="OQN12_000926"
     CDS             complement(39269..40003)
                     /gene="fabG"
                     /locus_tag="OQN12_000926"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1653358.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(40016..40945)
                     /gene="fabD"
                     /locus_tag="OQN12_000927"
     CDS             complement(40016..40945)
                     /gene="fabD"
                     /locus_tag="OQN12_000927"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1653359.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40961..41914)
                     /gene="fabH"
                     /locus_tag="OQN12_000928"
     CDS             complement(40961..41914)
                     /gene="fabH"
                     /locus_tag="OQN12_000928"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1653360.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41982..43052)
                     /gene="plsX"
                     /locus_tag="OQN12_000929"
     CDS             complement(41982..43052)
                     /gene="plsX"
                     /locus_tag="OQN12_000929"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1653361.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43133..43306)
                     /gene="rpmF"
                     /locus_tag="OQN12_000930"
     CDS             complement(43133..43306)
                     /gene="rpmF"
                     /locus_tag="OQN12_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1653362.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43358..43879)
                     /gene="yceD"
                     /locus_tag="OQN12_000931"
     CDS             complement(43358..43879)
                     /gene="yceD"
                     /locus_tag="OQN12_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1653363.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            44078..44662
                     /gene="yceF"
                     /locus_tag="OQN12_000932"
     CDS             44078..44662
                     /gene="yceF"
                     /locus_tag="OQN12_000932"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1653364.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44774..45733)
                     /gene="rluC"
                     /locus_tag="OQN12_000933"
     CDS             complement(44774..45733)
                     /gene="rluC"
                     /locus_tag="OQN12_000933"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1653365.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46306..49491
                     /gene="rne"
                     /locus_tag="OQN12_000934"
     CDS             46306..49491
                     /gene="rne"
                     /locus_tag="OQN12_000934"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1653366.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49687..50640)
                     /gene="flgL"
                     /locus_tag="OQN12_000935"
     CDS             complement(49687..50640)
                     /gene="flgL"
                     /locus_tag="OQN12_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS1653367.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50652..52294)
                     /gene="flgK"
                     /locus_tag="OQN12_000936"
                     /pseudo
     CDS             complement(50652..52294)
                     /gene="flgK"
                     /locus_tag="OQN12_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52360..53301)
                     /gene="flgJ"
                     /locus_tag="OQN12_000937"
     CDS             complement(52360..53301)
                     /gene="flgJ"
                     /locus_tag="OQN12_000937"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCS1653368.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53301..>53652)
                     /gene="flgI"
                     /locus_tag="OQN12_000938"
     CDS             complement(53301..>53652)
                     /gene="flgI"
                     /locus_tag="OQN12_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1653369.1"
                     /translation="DAKVVINSRTGSVVMNREVTLDSCAVAQGNLSVTVNRQANVSQP
                     DTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRALNALGATPMDLMSILQS
                     MQSAGCLRAKLEII"
BASE COUNT        13082 a        14291 c        13210 g        13069 t
ORIGIN      
        1 tatctgtcgt gcaatagatc tatttcttgc agttccatta ttacaggctc aagaagcgtt
       61 attaatgccg tggcaattct tgatttttgt ttgtcgcgtt cattgtcgcc aagtgtttca
      121 agccatatgc gcaatatttc cagcatgttt tcactgtgag aaagtgcaag aaatgcgcgg
      181 tctattgttt cgtggtaaat atcacgcata ttaatccccg tccgtcgttt ttcttaaaat
      241 aacctctgtc acgaaatcag cataatcggc ggcgatattc agtacatcaa gcccgttgat
      301 ttatattctc tcgtggaaag taagaaaaaa gccgctctaa taagttctgg cattgacgaa
      361 agcgcatcag ccgcatcatc aggaacgccg gaaaattcct gtttcaggga attaaaacga
      421 tcatcacgca taaccccccc cattttcaca atcggcaaca agaatatttt tagcgtcatt
      481 cagtgaccgc gttgcgatgt atcggataca ttccagggcg aactgtgtcc ggtctttctg
      541 ttcttctccc tgcgcaagct ctgccgtgca ttcaatatca ataagcgcgt gcatcagcgt
      601 agtgagtgcg gcggctgctg cgttcggtgt ggttttattg tgcataggtt tccccctgac
      661 gaatacgggc ggcgaataca gcaacacaac cggacgggca acggctacgc gcttcgcgtt
      721 ccgtccaagc ggttacgtgg atgatttggg attctccggc actcagtgcc agaaaacgcc
      781 acacaaaggc cgtttgtgtg tgtacaaggt gtggtatatg atttacggca accataacgg
      841 ctcctagttt acgttgttgg ttagaagccc cgttactgct cctacagtgc ggggctttgc
      901 tttttgtgtt gattgtgtgt aatgtgtcac tacacataaa tacattacct gaggtgtaat
      961 taacgtgtca acacacaaaa acgaaaggcg tggtaatcct ccgttccagt tccgtttaga
     1021 tcctgactta cgggaaaaga tggaaaaggc acaacaacag gacggcgatg attctcttgc
     1081 tgcctggata aagcgaataa tccgcaaaga attacagcaa cgcggcatag aacccaaagg
     1141 ataaaaacta tcagcgccgt ggtgtgaggt actacggcgc attgctttac aggcgcacac
     1201 aatgaccaac gaagaatcaa ccgaaaaaac atcacctgca cgaaaaagac gacgcagaaa
     1261 gaaagagcat gaatcagaac gattcgcgcc gtgttctttt gcccttgaga agtaccttaa
     1321 agaatactca tcaaaggaga gaacttcgca gttatggcaa cgcactgaac cagactgatt
     1381 gcattgccca ccagcctgat agcggctatc atccccgtgc ttatgtttgg gatcacacac
     1441 acaaacggcg cagcgggtta tctgttcaga aaggcggctc catttcgggg ccgctttttt
     1501 tatgcctgaa aaaccccaat tttgtggttt tccagtttca ccagggcgaa cgaatccccg
     1561 cccacgttct ggcgtatatt caatcttcat ggttatagct ctgtgttcag atgattgatg
     1621 tgtggcggct gcgtgccgcc agcgtgatta atgaactgcc ttgcagctat ccttccaggc
     1681 cagaacctcg gataaagacc agccaacgga acgaccgcca agtttacgac gtgatgggaa
     1741 ttgtccggcc ttttccaggc ggtagcggca tgagcggcta aggcctgtta gcttttcgca
     1801 ttctttttca cgtataaacc gatcagtgct taacactatt gccccctttc gtttcttaaa
     1861 gagttatttc gtgttctatt gcgttgggat gtgtctgatt gtgtcaggat gattcagagt
     1921 tggcaaatgt tgaggccgca tggtttacag aaagaggaat aatcaggata aaatccttta
     1981 aattcatgtt aatacaaagg cataaaatat tgtttcatgc cttttttctc gctatttaaa
     2041 gagtgattcg ctagtgtata aaaaatcagc aacacattaa aaatcagcta tttataaatc
     2101 tgtacacttt ttcacctctt gttcgtagtt gttccgcttt gttgctcatt gttgcacgtt
     2161 gtatctgttc gcatatccag tatgcgcata ctgaaaaaac acgaaaaaaa ttattttctt
     2221 ctggctactg gtagcgtggt tacgttttca tgtgttcccg ccagtatccc taaccgctcc
     2281 gtccacatat ccagcgcatt gcgtttagca tccagataac gggaatgatt ataaactcgc
     2341 tgcattcctg gcatctggtg gcctgtaagc tgctccacga cgtgaggatc cacgcctaaa
     2401 tcgttcagca tcgttgtaaa ggtgcgccgg atgtcatgta gtgaccagtg agggtgattt
     2461 agcctcctgt gcgctaatct gccgtactgc gacacgcttg tttcctgttt cacttccccc
     2521 agcaataagc ccgtgtgcct gttctgctcc accagctgcg tgacgaacgg cagtattgct
     2581 tccggtatgg gccggaatat tgccaccttc gttttgctgt gttctttcgg cacggtccag
     2641 agcatttcgg taaaatccca ctcgctgatc tccgataacc tcagttctac cgtccggcat
     2701 ccgaacacaa tcaggaggcg gattaacgcg atgtagtagg gggagaatat ttttttgtcc
     2761 agtgcctgca ataattcgcc cagttctttg gtgcttaaga cacgctcgct tatatccggt
     2821 tttttcccaa cgtccgccac actcatatca tcaagaacgt tgctgattgc atagcgccgc
     2881 ctccggcaga acttaagcgc ctgtttgcac gtctgtagca agaatccggc agtaacaggc
     2941 gttcgctttg ccacctggtc aaaacaggcc agccagtgcc gtagctcgca tttatccagc
     3001 ggcatagcac caatgtgctg tattacgtga ttattaaggc gctttttcag ggcggcataa
     3061 tccacgcggt tttcctttgc gtacgactca agccagtagg tgagcgcatc gccaaccgtt
     3121 accggcttta acgcttcctg tacggtgtaa ttaagctcat gacgtggatt ttcccctctg
     3181 ccagccatgc gcgacactgg gcggcttttt ccctggctga tttcaggttc agatcaggat
     3241 aatttcccag cttaatgcgt tccggtggtg ccccccttcc cgttccggcc ctgtaagtga
     3301 aataccaggt taaaaggcca ctggttgaat gcctgacgct caggtttcca ccgtcattaa
     3361 gaaaggctgt tttttgggcg ggtgtgccgt tgattttcct cagctgtgta tcgctcagtt
     3421 tgttaagtgc tctgctcata attttgattc cggtcatcac aattaactac accattaact
     3481 acaccgatcg ttgcacaaag ggctacaacg tgaaacaagc tggaacaagg aaaatcacaa
     3541 atctgtttat aatcaaaaac ataatgaaca atctgaaaca ttatgaaacg gcaaaaaaca
     3601 ctaattgata aaatatgtcc atacttcacg cattacgtta agcatccgtt ataatcggtt
     3661 gcagatacca gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat
     3721 tttcttgaac gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt
     3781 attgacccga tctctccaga cgagttggcg ggtctggcga tggaaagcga agttgacagt
     3841 cgactggtca gtcaccagga tggcaaatgg caggtcagcc acggcccgtt cgaaagctac
     3901 gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg cagtaaacca ctggcatgag
     3961 ccgaccgccg cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg
     4021 atgatttctt tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg
     4081 tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa
     4141 cagcactgcc cacatccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa
     4201 gagctggagc ctggtgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg
     4261 ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc caaatacgcg ggaattaatt
     4321 agcggatttg ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatccg
     4381 gatgttccac ctcgtgctca tcctgcggat gttctgccgc aagagatgga taaactgcgt
     4441 gagatgatgc tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt
     4501 atatcccagt cacgtcatga actggatatc gcgccgccgg aaccgcctta tcagccagat
     4561 gaaatctacg atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta
     4621 ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg
     4681 gcactggatg cactcgccag caacattgcg ctgactgcgg agaattttgg cgatgcgctg
     4741 gaagatccgt cattcctcgc gatgctcgcg gcgctggtca atagcgggta ttggttcttc
     4801 gaagggtaag tttggattta agccggatgc agcatcacat gccgcatccc ttttcgctta
     4861 cttccgttgc gccgttaact cggcaatacg gacgatcacc tgcaccgctt tttccatacc
     4921 ttccagaatc acaaactcat gcttaccatg atagttgtaa ccgccagtga acaggttcgg
     4981 gcaaggtaat cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag
     5041 ttccggttca atatcgcagt cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg
     5101 ctcaaccact ttctcgcgca tattgtagta actgtcttca atcaccaatt caatgtagca
     5161 atcaggatgt aaccctttgc ccactttttt ggcgatctcc atcattttac gtttacgcgc
     5221 ttcaaactgt ttacggtcga aatcacggat gatgtagtgc atatcggccc gttcaacggt
     5281 gcctttcatg ctcgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc
     5341 atccgccgga acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc
     5401 tttcgccgtg cccggatgaa cattgttacc gacaattttt atattgaccg atgcggcgtt
     5461 gaagttttca aactccagtt cgcctacgcc accaccgtca acagtgtaag cccagcgggc
     5521 atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa
     5581 ggcgacgcga atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat
     5641 gatttctgca atacctgctt tgtcatcagc acctaacaag gttttaccat cggtggtaat
     5701 caacgtctga cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc
     5761 gccgataccc agcgcaatat cgccaccgcg atagttttca acaatttgcg gattaacatt
     5821 tttgccgctg caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc
     5881 gccagggacg ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt
     5941 gataagcccc atttcttcga gctgctcttt cagcagatgc aataacttcc attggccttc
     6001 cgtgctggga acctgtctca cccctgcttt tgattgggta tccagagaca cgtagttcaa
     6061 aaatcgctca agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag
     6121 aagcaagaaa agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta
     6181 tcagcattac cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt
     6241 caagcggttt gccgtagaat taccgccctc attaagagtc accaaggtgg ttaaccacaa
     6301 accccgcatc ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa
     6361 caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt
     6421 aaagaggtca ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt
     6481 ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt
     6541 gattccggac gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc
     6601 tatgtgaaca ctgttttcca aagctatgca cttttccccc acatgaccgt gttcgaaaat
     6661 gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg
     6721 gaagccctgc ggatggtgca gctggaaacc ttcgctcaac gtaaaccgca tcagctctct
     6781 ggtggtcaac agcaacgcgt cgccattgct cgcgcggtgg ttaacaagcc tcgtctgttg
     6841 ttgctggatg agtcgctctc agcgctggat tacaaactgc gtaagcaaat gcagaacgag
     6901 ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt tcgtgactca cgaccaggaa
     6961 gaagcactca ccatgtcgga caggattgtg gtgatgcgcg atggtcgcat tgagcaagac
     7021 ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt ttgttgccgg gttcattggc
     7081 gaaattaata tgtttaacgc cacggtgatc gagcgactcg acgagcagcg cgtacgcgcc
     7141 aacgttgaag gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa
     7201 ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac
     7261 gctgaagggc tgattggtta cgttcgtgag cgtaactaca aaggcatgac gctggagtcg
     7321 gttgttgaac tggaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgat
     7381 cctgactttg accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag
     7441 gtcgtactgg ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt
     7501 cggttggctt gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt
     7561 gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg ctggataact acacgcgtct
     7621 gctcgatccg ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac
     7681 cctcgcctgc ctggtgctgg gctatccatt tgcctggttt ctggcgaagc tgccacacaa
     7741 ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc tggaccaact cattgattcg
     7801 tatctacggg ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg
     7861 gctgggcgtt atcgacacac caatccgtat catgttcacg cccagtgcgg tgattatcgg
     7921 tctggtttac attctgctgc cgtttatggt gatgccgctg tactccagta tcgaaaaact
     7981 ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc agcaagttac agacttttat
     8041 ccgcatcatt attccactga cgatgccggg gattattgcc ggatgtctgc tggtgatgct
     8101 gccagcgatg ggcctgttct atgtgtccga cctgatgggc ggtgcgaaaa acctgctgat
     8161 cggtaacgtc atcaaggtac agttcctcaa tattcgtgac tggccgtttg gtgcagccac
     8221 cagcattacg ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg
     8281 tttgctgaat aagaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg
     8341 accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac
     8401 agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg
     8461 aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt gttttcggcg
     8521 acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgc
     8581 ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga tgtcaccaga tatcgtgatg
     8641 gcaatttcgc tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg
     8701 ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg tgacggtgta ttcgcgcctg
     8761 aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag cgaatttacc
     8821 attctgcgaa aaatcattct gccactggca atgccagcgg tggcggcggg ctgggtgtta
     8881 agctttaccc tgtcgatgga cgatgtggta gtttcttcat tcgtcaccgg accaagttat
     8941 gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac
     9001 gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt
     9061 gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc
     9121 tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaaca
     9181 cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca
     9241 aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc atgtacgcca
     9301 agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc tattacgtcg
     9361 ataaaatgcg taaagagggg atgatccaga agatcgacaa gtcgaagtta acgaacttca
     9421 gcaatctcga tccagacatg ctcaacaagc cttttgaccc gaataacgac tactccattc
     9481 cgtatatctg gggtgcgacg gcgattggtg ttaacggtga tgcggtggat ccgaaatctg
     9541 tcaccagctg ggccgatctg tggaagccag agtacaaagg cagcctgctg ttgaccgacg
     9601 atgcccgtga agtgttccag atggcgctgc gtaagctggg ctactccggt aacaccaccg
     9661 atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtcgcag
     9721 cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac ctcggcatga
     9781 tctggaacgg ttctgctttc gttgcacgcc aggcgggtac gcctattgac gtggtgtggc
     9841 cgaaagaagg cggcatattc tggatggaca gcctggcgat cccggcaaat gccaaaaaca
     9901 aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg
     9961 ctgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta agtccagaag
    10021 tggcaaacga taagacgctc tacccggatg ctgaaaccat taaaaatggc gaatggcaga
    10081 atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac
    10141 gttaattcag caagcgggcg gtggtattac cgcccgtttt ttagttagca aggatgtggc
    10201 taatgagtca ggacagtaaa gttttatccc gctctttcgt cggtattgtg gtccgcttgt
    10261 aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc tccagcaact
    10321 tttcaacaaa ttctggcacc acctggcttg ccgggccgta atatttctcg gcaaattcat
    10381 taccaacctg actcggttca agattcagtt caacggtgtg agcaccatgt agtttggctt
    10441 cgtgtacaaa cccagccgcc ggatagacat gcccggaagt accaatggca ataaagacat
    10501 ccgccatcga caacgccata taaatttcat ccatgccgag tggcatttcg ccaaaccaaa
    10561 cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg gcatttatct tctggcgtaa
    10621 cgtctccggt ccagtcgaga acctgaccac tttgcgaaca acgaactttc agcaattccc
    10681 catgcatatg aatgacattg gtattacctg cgcgttcatg caggttgtct atattctgcg
    10741 ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt agccagcgca agatgcgcgg
    10801 cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa aacgcttgca
    10861 ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc cgatgttctt
    10921 cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa attcctgccc
    10981 ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta tctctgaaaa
    11041 aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt tttacgaaaa cgacttaacc
    11101 gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga tgtaggaagg
    11161 ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgc ggaacacgag
    11221 ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg cgttgtgatt gccgggaaat
    11281 tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg atatttccca
    11341 gagaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc tcatcgcctt
    11401 gatcataaag cgcaataatt tcgggagcct gcaacggttg atgataatag tgttgataca
    11461 gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga ccacagccgc
    11521 agcggcgtaa cgggaaatcc aaccccatca tggttaacgc atcaaccggc agacgcatat
    11581 ggccaaactc gccggtaatg tagcttttac cagtaatcgg tttgccgttg aaaaccagcc
    11641 cgccgccaac gccggtgccg agaattaacc ccatcaccag tggatattgc gtaaattcat
    11701 catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt acatcgcgat
    11761 caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct ggcagcgggg acattagcgg
    11821 catacagctt accatcttct gtttccggca ttcccggaat accgatgccg acagaacctt
    11881 tacagccaaa acgttggtca gcttcagcta ccagctcaca cactgcatct aaaaatgcgt
    11941 catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga ccgctatcaa
    12001 acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt gccgctcctt
    12061 gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat attactggcg
    12121 cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac cagtacgtag
    12181 aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa taaaataaat atcgctggag
    12241 aggaactgat gaccgatcag cttttcaatc cactcaataa tcggggtaag ttgcagtgaa
    12301 acaactacgc cgataaccac cccacacagg ctgccgaaca gccctgccag caatccatac
    12361 cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa tactgcgata
    12421 tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa acaggccacg
    12481 ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat atcgcgatac
    12541 atatagccgt aagtaccaat ccaactttta atataaacat agctgttggt cacttcaccc
    12601 gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc aatacctgac
    12661 acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat ggcaaaactg
    12721 tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt gcaaacgcac acgttttggc
    12781 tgcatcagtt tatgctcggg attcgagttg gggatcatga tcgacaccca atcgccctgc
    12841 ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc gcccgcttta
    12901 aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg cgctcagacg ctgttcctgt
    12961 tgtgggttaa cgcccttcac ctggattgcg cgcagattcg ctccactttc caccagaccg
    13021 gtgaaattga tatacggcgc agcggcggca atacccggca ctttctgcac gttatccagt
    13081 gcttcctgcc agttagtcca tggctgattt accgcttcga tttcgccatg cggcaccacc
    13141 gccagaatgc ggttattcag ttcgcgttca aagccgttca tcgcgcttaa gccgacgatc
    13201 aataccgcca cgccgagggc aatgccaatg gtagaaatga cagagattag cgacaccatg
    13261 ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca ataacgataa aggcatcgcc
    13321 attactccgc ccccatcagg ctcagttccg ccgtcagacg tccatcacgc atttccagtt
    13381 ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac caccaggaaa gcggtgccct
    13441 gcaagcgatt caactcccca agcaactgga agatgctgtc ggcgttacgc gcatcgaggt
    13501 taccggtagg ttcatccgcc agtaccagac gcggattatt gaccagcgca cgggcaatcg
    13561 ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca cgatgctcca
    13621 gccccaccgc ttttaacatc tccagtgcac agctgttgat ttcagcgggc tttttcttgc
    13681 caatcagcag tggcatagcc acgttttcca gggcagtaaa atccggcagc aggtggtgaa
    13741 actgataaat aaagcccagc ttctggttgc gcagttctgc tttcgccgct gaagacagtt
    13801 tgctcatcgg ctgaccgtta aagatcacat cgccggaggt tggcgtatcc agcccgccta
    13861 acaggtgcag caaggtactt ttaccggaac cagagctacc gacaattgcc atcatttcac
    13921 cttcgcccac gctgaaactg acattgagca gcacatcggt ttgcacactg ccttcctgat
    13981 agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa agcctcagcg
    14041 ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc gatagccatc
    14101 gccaccagcg caataacaat gacctgtaaa ggttcgatag ccaccggcag cgccgcgcca
    14161 tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag cagtgcgcca
    14221 agcgccgctc cgaggattgc accgataatc ccggcgctag ccccttgcac cataaagacc
    14281 atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgcgacttc gccctgcttc
    14341 tccatcacca tcagccccag cgaggtaata atgttaaacg ccgcaacagc gacaatcagg
    14401 ctcagcagta agcccatcat atttttttcc atgcgtacgg cctggaacag ctcgccttta
    14461 cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact taaagagtca
    14521 actctcagcg gctcatccag ccacaaacgc cagccggtaa tattggctgc cggataacgc
    14581 atcagacgcg atgcatcctc aatattcacc agcatttcat agccatcgac ttcactgttg
    14641 gcggcgaaag tgccaatcac attgaacagg cgctgacttg gaatacgccc catcggcgtg
    14701 aactggctgg cagatggcac catcacgcgg atttgatcac cgcgattgac gcccaactgt
    14761 gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc agtttgtttc
    14821 acattgacca gatacggcgt tagtggatct ttttgcgccg ggtcgatacc gagcatcacc
    14881 ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat aggtgcgacg
    14941 cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt aagagagcca
    15001 tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg cagctcgcgc
    15061 tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag ggtaatgccg
    15121 atggtagaaa gccaggagac gaaacgaccg aagcgatcca ctgcacgccc acgcatgtaa
    15181 cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa
    15241 agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa gtgatcgtca
    15301 gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatgttt tatcatcaat
    15361 cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc attacacctc
    15421 gggtcataac cagcaggatc aaccgcatgt aaattaaaaa agagcgtgtt gttctgatga
    15481 aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg gtggtgattt
    15541 atcactcggt cattaccttt tatccgcata tgaccacttt tcagcatccg ttatcggaag
    15601 tcctgagcaa atgctggatc tatttcaatc tttaccttgc cccctttcgt atgccggttt
    15661 ttttctttat atccggctat ttgattcgcc gctatatcga cagcgtaccg tggggaaatt
    15721 gtctcgataa acggatctgg aacatcttct gggtgctggc gctttggggc gtggtgcagt
    15781 ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta agcaatgcct
    15841 ccaatgccgc ttatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca
    15901 ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa atttttagcc
    15961 gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt aatttcgttc
    16021 ccacgccgtg gtggggaatg aacagtgtga tacgcaattt gccttattac agccttggcg
    16081 catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc
    16141 tgatggcttc tttgcttacc gttctggcgg tcggtgcctg gttgtttact atctcgctgc
    16201 tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt ttatcagtac gagcaacgat
    16261 tcggtatgcg ctccaccagc ctgctgaatg tgattggctc caacaccatt gctatctaca
    16321 ccacccatcg cattctggtt gaaatattca gcttaactct gcttgcgcaa atgaacgcag
    16381 cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttatcccttt gtcagtttgt
    16441 tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc
    16501 tgttgttctc cccgccttca ctgcccgctg ccgtcagtta ctctcgttaa accatcaccc
    16561 cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac agcattgctt
    16621 atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac aaccgttatg
    16681 cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag
    16741 ttaaccggcg cagcctgtgc aacgctggta gcggaaattg ccgaacgtca cgccggtccg
    16801 gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga aatcagccag
    16861 ttcaccgatc agatggtgat gaatctggcg gactgggaaa ctcttcccta cgacagtttt
    16921 tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct accgacgatg
    16981 cagcgtggca tactgattgt tccggtgaat acgcttatgc agcgcgtttg cccacacagt
    17041 tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta
    17101 cgaacccaac tggacagcgc cggttatcgc catgttgacc aggtgatgga gcacggcgaa
    17161 tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct gccttatcgt
    17221 cttgatttct ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga cagccagcgc
    17281 acgctggagg aagtagaagc gatcaatctg ctgcccgcgc acgaatttcc gaccgataaa
    17341 gcggcaattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg
    17401 gaacatattt accagcaagt gagtaaaggc acattacctg ctgggatcga gtactggcag
    17461 ccattgttct tcagcgaacc actgccgccg ctgttcagtt atttccctgc caataccttg
    17521 ctcgtgaata ctggcgatct ggaaaccagt gccgaacgtt tccaagctga cacgctggcg
    17581 cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt tgccaccaca atcgctctgg
    17641 ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgagtgca gctaaaaact
    17701 gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact gccagacctg
    17761 gccgttcagg cgcaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagactttc
    17821 gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg
    17881 ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt
    17941 gggcgttatc tgatgattgg cgctgccgaa catggttttg tcgataaggc gcgtaatctg
    18001 gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg cccgccgtcg tcaggattct
    18061 cgccgcacca tcaaccccga tacactgatc cgtaaccttg cggagctgca tattggtcag
    18121 ccggtggtcc atctggagca cggcgtcggt cgctatgccg gaatgaccac gctggaagct
    18181 ggcggcatta ctggcgagta cctgatgctc acctatgcca acgacgccaa actgtatgtt
    18241 ccggtgtcgt cactgcatct gattagccgt tacgcaggtg gcgcggaaga aaacgccccg
    18301 ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg
    18361 cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc
    18421 gcgtttaaac acgatcgtga gcagtatcag ttattctgcg acagcttccc gtttgaaacc
    18481 acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca gccgctggca
    18541 atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc
    18601 gctttcctgg cagtagataa ccacaagcag gtagcggtgt tggtgcctac cacccttctt
    18661 gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa
    18721 atgatctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa
    18781 gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa
    18841 gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc
    18901 attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc gatcccacgt
    18961 acgctgaata tggcaatgag cggaatgcgt gacctgtcga ttatcgccac gccgcccgcc
    19021 cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt ccgggaggcg
    19081 atcctgcgtg aaattttgcg cggaggacag gtttattatc tctacaatga tgtggaaaac
    19141 attcagaaag ccgccgaacg gctggcagaa ctggtgcctg aagcacggat tgccatcggt
    19201 cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt
    19261 ttcaacgtgc tggtttgtac caccattatc gaaaccggga tcgacatccc gacagccaac
    19321 accattatca ttgaacgtgc ggatcacttc ggtctggcgc agctgcacca gttacgcggt
    19381 cgcgtcggac gttcacatca tcaggcatat gcatggctgc tgacgccgca tccaaaagcg
    19441 atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggggca
    19501 ggttttgcgc tggcaacgca cgatctggag attcgcggcg cgggtgaact gcttggcgaa
    19561 gaacaaagcg gttcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac
    19621 gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa
    19681 acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcatccc tgacgtgaac
    19741 acgcgtctgt cgttctacaa acgtattgcc agcgccaaaa cggaaaacga actggaagag
    19801 atcaaagtcg agcttatcga tcgcttcggc ctgctgccag atccggcgcg taccctgctg
    19861 gatattgccc gtctgcgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat
    19921 gagaaaggcg gggtgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt
    19981 ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg cctgaaattt
    20041 attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa
    20101 ctggaagaga acgcgatcgc ttaatgtaaa tcgggggcgt ttctgcgccc ccatctgcaa
    20161 catttacaaa ttctttgcac tttcctgcac tatccgacac tgtcaccatc cataattcag
    20221 gcttatctgt ttattacaat aaccttatat ttattatgta tttttggtga tgatcaaaac
    20281 gcgtttttct cgctggctaa cgttttttac gttcgccgct gccgtggcgc tggcgctacc
    20341 ggcaaaagcc aacacctggc cgctgccgcc agcgagcagt cgtctggttg gcgaaaacaa
    20401 atttcatgtg gtggaaaatg acggtggttc tctggaagcc atcgccaaaa aatataacgt
    20461 cggctttctc gctctgttac aagctaaccc cggcgttgat ccttacgtac cgcgcgcggg
    20521 cagcgtgtta acgatcccgt tgcaaaccct acttccagac gcgccgcgcg aaggcattgt
    20581 gatcaacatt gcggagctgc gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt
    20641 gtatccaata ggtattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac
    20701 cgtttcagac aaacgtgcaa acccaacctg gacgccaacg gcaaacatcc gcgcccgtta
    20761 taaagcacag ggaattgagt tgcctgcggt agtgccggct ggaccggata acccaatggg
    20821 ccatcatgcg attcgtctgg cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc
    20881 cgatttcggc attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat
    20941 caaaacactc tttagccagg tcaccccagg caccaaagtg aatatcatca acactccgat
    21001 aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga
    21061 gaagattgat gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt
    21121 taaagatgca gcacaaactg acgctgaagt gatgcaacat gtgatggatg tccgttccgg
    21181 gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa tcctggataa
    21241 aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg
    21301 agggttaatg cttatttata aattactgct gttccatgga gggtattcgg accggttaca
    21361 gaagtaatac ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc
    21421 agcgatccca gatttgtccc cgcgttagca ctgatagtac cgactttttg ttggccttct
    21481 ggcgttgact gaacttcgac agccgcaaag ttggcaaatg acatggagct taaaatcgcc
    21541 gcagcgatga ggtttttacg tttttcataa tagtggcctt atgcagatga atgacgtcgc
    21601 aagaaattac ttaacgatcg ataactaaat gatagatgtg atctggatca catacaagat
    21661 attttttata acaatcatta attaatttaa aaatctttaa atttcagtca aatagaaagc
    21721 aattattttt atgggatctg gactggtgaa cggaggtgat tatttttata ataacctttc
    21781 attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa
    21841 aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg ccatgaaatt
    21901 gttctggcaa cacggttatg aagcgacttc tcttgcggac ctcgtcgaag caaccggagc
    21961 caaagcgccc acgctatacg cggaatttac caacaaagag gggttatttc gtgccgttct
    22021 cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa
    22081 aagcgtggag tctgcgctgg ctgactattt tgcggccatc gccaactgct ttaccagcaa
    22141 agacaccccg gcaggctgct tcatgatcaa caactgcacc accctctccc cagattcagg
    22201 agatatagcc aatacgttga aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt
    22261 tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga cacatctggc
    22321 agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct
    22381 ggaaaaactg atgcagattg ccggaacgac tttgcgttta tggcccgaac tggtgaaata
    22441 agtcgcaaaa aaggcccctc agcctgagcg aatgaggggc caaattgcag agtacagcct
    22501 tgttatactt tgttattcaa aatcagttgc ccgttactat ctagcgggat ctgcgtaccc
    22561 ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt cacatcataa
    22621 ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa
    22681 gtatcgcttt cctggagaga gccctggatc tggttaccgg catatccacc acccagcgcc
    22741 cccacaacag tggcgacatc tttaccgcga ccaccaccaa actgatgccc tatcacgcca
    22801 ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac cggtcgacga
    22861 tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc cttgattggg
    22921 gttgcagaaa caacctgagc gtattgcggg ccccgttcaa acacgttcag actggccact
    22981 gccgctacgc ccaacgcagc tgcgacacca atcccgatac ccgccaacat tgatttattc
    23041 acgggaacac ctccttcttt gcagttatgc caatcatgca agcgaaggag aaaagcagct
    23101 atcagacaga agatgaaaag cgcgggaatg actggaaata cttaaggatt cggaggagtc
    23161 tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct cgtaccattt
    23221 gtccagggcg gataaggcgt ttataccaca tccgccagtg tacgtcgatt aatgcaactt
    23281 caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa
    23341 gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa agcgcgcaat
    23401 tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac caacggtgga
    23461 gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt tcagcggctt
    23521 accgttcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt gtgcagcctg
    23581 agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc
    23641 gtaaatgtct ggatcgcgag tggtttgcag cgtcggttcc accaccagct ggttgatacg
    23701 gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca
    23761 taccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc
    23821 actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc
    23881 cgcagtagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt
    23941 tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt gcttgatcgc
    24001 gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt
    24061 cactttgcca tttgcgccca ggttggcaga gtatttcagg aacagattca gcatctcctg
    24121 gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacgcctgg
    24181 cgtattgaaa tcgttagagg tgctacccag cgccatgacc agggtgtcat aggcgatttt
    24241 acgttccgga accaacagtt cacctttctc gtcgcgtagt tctgcaatag tgattgtttt
    24301 cgcttcacga tcaatatcaa tgacggaacc caactggaac tggaaaccat gattgcgcgc
    24361 atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg
    24421 cagcagtggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt tggctttttt
    24481 cttacgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc
    24541 gacaatcaca atctttttca atggcgtagt caacgtgacc cccttaaatt tattaaccaa
    24601 ttgttaatta atggttatta acatagctct taattaacaa caggttacag caatgaaaat
    24661 gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat
    24721 caagttttgt tgcttaaaag ataggtaact ttaccggggg agatcaaaaa atagagttta
    24781 ttgtttatta tttgagcaaa ttcggaaggg taatataggg tgaagcgtat atcgagggcg
    24841 tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg actgtcggat
    24901 gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac cggcaaggtt
    24961 acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg ctgtaaatgc
    25021 ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc gtaataatga
    25081 tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga caaaataacc
    25141 agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc aatatcggca
    25201 tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa attagggttg
    25261 aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta tccgcccagg
    25321 ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt gtccacttct
    25381 ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat caagcgtaca
    25441 tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact gttagagtca
    25501 aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt gacctgcttt
    25561 ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt tccagcgagc
    25621 cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg taacacgttc
    25681 tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat tattgcagac
    25741 caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat aactgcccat
    25801 aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa aacccagttc
    25861 ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg gatcaacatc
    25921 actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga ttaaggaact
    25981 gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc
    26041 tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat
    26101 tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt tttgtggatc
    26161 ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca gcactggcgc
    26221 aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac ccgcctcttg
    26281 cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg ctgccggtaa
    26341 gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta ccgccaccac
    26401 cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact gggcaggatc
    26461 atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca gtatttcacg
    26521 ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca cactgctctc
    26581 cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat
    26641 cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc agccagggaa
    26701 accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt
    26761 cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg
    26821 cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat gcgctatgca
    26881 ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt cgtggttccc
    26941 gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc gccggatcgc
    27001 tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca aaacgtggtt
    27061 gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta
    27121 ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg
    27181 caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg
    27241 catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac
    27301 taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg
    27361 acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt acctgaccag
    27421 ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg
    27481 ccagaggcgc tggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct
    27541 ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac
    27601 agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt
    27661 tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc
    27721 aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt
    27781 gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg atcttcgtgc
    27841 tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt
    27901 tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt
    27961 tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt
    28021 tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg tgcgccccca
    28081 gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta acgctgcgtg
    28141 gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa
    28201 cggtaacggg ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt tgaatatcag
    28261 acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca
    28321 ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta
    28381 acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc
    28441 cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg
    28501 tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg
    28561 ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga
    28621 cacgtcgttc acagtcggaa tgagaatatt cggaatgatc agaatatgcg ttggcgcttg
    28681 cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat
    28741 ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa
    28801 atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag
    28861 ttaaacctgc tctttcaatc ttaactcacg atttcttaag caaaaaatcg ttttgctgcc
    28921 gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt
    28981 tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttgcat ttgcatttgc
    29041 agcaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga
    29101 cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat
    29161 atcaagtcat caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt
    29221 ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag
    29281 ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc
    29341 agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag
    29401 atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc
    29461 aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg
    29521 ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata
    29581 tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg
    29641 gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gacctgtcgt gaatttaaag
    29701 gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa
    29761 gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg
    29821 actcatggct ggaccgctac aatagtgaaa agaccttctt ctcgggcatt gtcgatgctg
    29881 atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata
    29941 gcccaacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc
    30001 gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta
    30061 tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg
    30121 aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta
    30181 tgctggttgg gccatacagt aattatggac ctggctttga tcatgtcggc ggcaccggtt
    30241 ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat
    30301 tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt
    30361 cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa
    30421 tttcccacaa gccgactggt catcacagag cctggcgcag gacgatacca cacatatgaa
    30481 atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc
    30541 acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac
    30601 gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac
    30661 ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac
    30721 cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac
    30781 gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc
    30841 cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga
    30901 atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta
    30961 tcttgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa
    31021 aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa
    31081 ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca
    31141 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
    31201 acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat
    31261 cgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc
    31321 tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt
    31381 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg
    31441 aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc
    31501 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
    31561 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
    31621 tgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
    31681 cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga tcggtaacag
    31741 ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga
    31801 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc
    31861 gtggatgatg tacaggatcg gcgcaacgaa catgaagaag aactcgatcg gctcggtgat
    31921 accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcacggtt
    31981 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
    32041 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
    32101 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
    32161 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
    32221 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
    32281 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat
    32341 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
    32401 gaaacggtta aacatgtacg ctgcaatcgc accggagata atccctccga gtacgccagt
    32461 atccgccaag tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
    32521 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
    32581 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
    32641 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
    32701 cgcggaaccg acgcccagca gaatacctgc gataggtagt acggataccg gcagcatcag
    32761 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
    32821 tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt
    32881 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg
    32941 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
    33001 atatgtcaca atatttcaaa cgcggttatt caccgcccaa ttttcggcgg tgaactttac
    33061 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
    33121 ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
    33181 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
    33241 attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa
    33301 tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac
    33361 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
    33421 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
    33481 cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
    33541 aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata
    33601 ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa
    33661 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
    33721 gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac
    33781 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
    33841 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
    33901 gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac
    33961 gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt
    34021 cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg
    34081 ccagttcggc gaccagtccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc
    34141 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
    34201 gattaagggc cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct
    34261 gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag
    34321 gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
    34381 gtgaaagcca agtcacggcg tactgttctg gaggcggcgc aaggtaatgt aaccgacaac
    34441 gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt
    34501 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg
    34561 cagcatcggt tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt
    34621 cggtgacctc acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg
    34681 tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac
    34741 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    34801 catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat
    34861 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    34921 ctccttcacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg
    34981 ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg
    35041 ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg
    35101 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt ctgggcgaaa
    35161 atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc
    35221 gccctgatac gcctgagtgg aaagatcgtg gcgatcgcca atcacccagg tgccgttagc
    35281 cagcgcaggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    35341 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    35401 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    35461 cagttgctcg agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag
    35521 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    35581 gcacagactt gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    35641 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    35701 ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg
    35761 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    35821 cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa
    35881 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    35941 gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    36001 ctttcaccat tttcttgtga actcgcttga gtaacgcgac atcggtggta ttggcggtat
    36061 acatcaaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg
    36121 cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac
    36181 gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg
    36241 cttctttacc gctttccaac aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    36301 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa
    36361 cccgtgggcg attgatgatc ttatcagcat aaagctgttc accgagcgcc agacgtccgg
    36421 tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat
    36481 ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    36541 taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    36601 atataacgtt gctgacgaaa aggtgacatc gccacaggca cgtacgggca tcactggcat
    36661 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac
    36721 ttcgacaagc tgataagagg attgtgccag taaacggata cagaattgtc gcataatgcc
    36781 gttaacacct gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc gccagaacaa
    36841 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    36901 gtttgtctgc tcaagatgag agcgaatcaa tacttgctca agccgattaa gatgtttaat
    36961 acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc
    37021 gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgctgg
    37081 tccgctgttc aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag
    37141 cacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa
    37201 gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgcaggatat gcgccgataa
    37261 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    37321 tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc
    37381 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    37441 gctaagaaaa aaggcccgca agcggacctt ttatatgggt ggaaaatgac aacttagatc
    37501 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    37561 tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc
    37621 gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    37681 atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt
    37741 accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta
    37801 tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg
    37861 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    37921 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    37981 gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc
    38041 agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    38101 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc
    38161 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg
    38221 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    38281 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    38341 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    38401 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    38461 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    38521 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    38581 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    38641 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    38701 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaagg agggaaaaat
    38761 gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca
    38821 cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag
    38881 cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg
    38941 tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct
    39001 gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac
    39061 tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga
    39121 aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta
    39181 ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg
    39241 caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca
    39301 aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga
    39361 tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt
    39421 catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag
    39481 taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt
    39541 tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag
    39601 tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga
    39661 aaacagatga aaggttagtt tcgataatat cgttccactc ttcatctttc attcgcatta
    39721 acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg
    39781 cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac
    39841 ctttgccgtt ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag
    39901 tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc
    39961 ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa
    40021 gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga
    40081 caatgcgttt cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta
    40141 cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac
    40201 gtaccagtgc gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca
    40261 caacagaaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg
    40321 ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt
    40381 ttgcgccagc agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa
    40441 taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt
    40501 cttcacacgc tttcgcaata ggcgcatcat ccagaccgat gattgccgcc atagcgcccg
    40561 tgccttccgg tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat
    40621 cagcgaaatc aatcacgcca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg
    40681 ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg
    40741 cagtcaacag cgctggctgg gtttgccagg ttttattcag ttcttcagct ggcccctgct
    40801 gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga
    40861 caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag
    40921 ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcag
    40981 agccccaagt gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc
    41041 gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt
    41101 ggcgatccag cgtcaccacg acattgtcca tcgacatacc gagttttttc gccgttgcac
    41161 tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat
    41221 tattcgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga
    41281 cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacgcgg tcggcgtttg
    41341 gcagcgtcag caattcacca taactaccgt cggcatgcag atgggtggaa atgattcccg
    41401 gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc
    41461 cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact
    41521 tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcac
    41581 aggctgctgc aacgtcaaat gccgggcaac ctttaatgtc caacatgctt tgaatctgac
    41641 aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct
    41701 ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg
    41761 aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc
    41821 actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca
    41881 gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata
    41941 taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt
    42001 ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt
    42061 cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct
    42121 cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg
    42181 ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa
    42241 cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga
    42301 gaaaggaaca tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg
    42361 tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata
    42421 taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact gtcaagaccc
    42481 tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc
    42541 tcttcagcca gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg
    42601 acgttggccc ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc
    42661 accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc
    42721 agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc
    42781 agggccatac gcattgaact cccacgactg gcgcggatag cttgcgaagg ccgggcatca
    42841 ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct
    42901 ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa
    42961 ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc
    43021 atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt
    43081 ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag
    43141 cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt
    43201 caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac
    43261 gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc
    43321 aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta
    43381 atacggcaaa tgggtttggc ttttgcactt cttcaggcag ttcaccaaag accatgtccg
    43441 cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga
    43501 tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt
    43561 catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg
    43621 ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca
    43681 ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc
    43741 attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg
    43801 tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg
    43861 gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga
    43921 aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt
    43981 attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat
    44041 ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc
    44101 cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc
    44161 acctgaggtc gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact
    44221 ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc
    44281 agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc
    44341 ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt
    44401 gtttaattcg gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg
    44461 tcacctaagc gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc
    44521 gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga
    44581 ccctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg
    44641 gaaaaacccg ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat
    44701 cggtgcagac ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc
    44761 ttatatgctt gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat
    44821 ccatcggcgc ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg
    44881 cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt
    44941 cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac
    45001 ggatctgatg agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc
    45061 gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac
    45121 gttcgccgct ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact
    45181 gccactgacc gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct
    45241 catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc
    45301 ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt
    45361 cgataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca
    45421 gcaccaggat atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca
    45481 gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct
    45541 catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac
    45601 ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa
    45661 agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag
    45721 tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc
    45781 tatgcttata tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt
    45841 attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa
    45901 aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc
    45961 ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc
    46021 aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac
    46081 cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg
    46141 tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg
    46201 tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt
    46261 tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta
    46321 atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat
    46381 gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa
    46441 atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac
    46501 ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat
    46561 ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat
    46621 aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc
    46681 tatctggttc tgatgccaaa taacccgcgc gcaggtggca tttctcgccg tatcgaaggc
    46741 gatgaccgta ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg
    46801 cttatcgtgc gcaccgctgg cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc
    46861 ttccgcctga aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc
    46921 ctgattcatc aggagagcaa cgtaatcgtt cgcgccttcc gcgattattt acgtcaggat
    46981 atcggcgaaa tccttatcga taacccgaaa gtcctcgaac tggcacgtca gcatatcgct
    47041 gcattaggtc gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg
    47101 ttcagccact accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg
    47161 ccatccggtg gttccattgt tatcgacagc accgaagcgt taacagccat cgacatcaac
    47221 tccgcacgcg cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa
    47281 gccgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc
    47341 gacttcatcg acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa
    47401 gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg
    47461 gaaatgtccc gccagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg
    47521 cgctgctccg gtaccggtac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt
    47581 ttgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg
    47641 ccaatcgctt cttatctgct gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt
    47701 caggacggtg ttcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac
    47761 gtgttgcgcg tgcgcaaagg ggaagaaacg ccgaccttaa gctacatgct gccgaagctg
    47821 catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa
    47881 caacctgcac tggcaacctt tgccatgccg gatgtaccgc cagcgccaac cccagctgaa
    47941 cctgccgcga ctgtcgtagc cccagcaccg aaagctgcaa cggcaacacc agcagctcct
    48001 gcacaacctg ggctgttaag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt
    48061 gaagaagcca aaccgaccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa
    48121 caggatcgtc gcaaacctcg tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc
    48181 cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag
    48241 gcacagcagc aaactgccga gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa
    48301 gcacgtacca ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat
    48361 gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag
    48421 gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc
    48481 aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt
    48541 gaagaaactg ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg
    48601 gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat
    48661 gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg
    48721 cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca
    48781 atgccgttga ccgtagcgtg cacgtctccg gaattggcct ctggcaaagt ctggatccgc
    48841 tatccaattg tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta
    48901 caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc
    48961 gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg
    49021 caaccggaag tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag
    49081 ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa
    49141 ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag
    49201 gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc
    49261 gcagcagaag ttgaaacggt ggccgcggtt gaaccagaga tcaccgttga gcataaccac
    49321 gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcgtcac
    49381 agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg
    49441 gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct
    49501 caaagtaatc aagccctggt aactgccagg gcttttttat ttcatgtttg aatcaacata
    49561 ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc
    49621 cgccccggcg atcccagagc gggcagacaa aacataccca gtttcatgat atgttttaaa
    49681 gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa atgctttata
    49741 cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac
    49801 atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac ctaaggaatc
    49861 cagcgactcc agtttgttca gctgcgtgcc taattccgcg cgaacggtaa ggacattatt
    49921 cagcgagttt ttcagtccac ggttggtttt atccaatgcc gcagcggcga tttctttatc
    49981 cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa
    50041 aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg taatactgtc
    50101 gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga cctgttgctt
    50161 aatactttct gttccgccaa cataatcacc gttagcctca ctaaacggcg cagtctctgt
    50221 tttataaccg gcaaaaatgt agcgcccatt accgtcagtg gtgttcgcca gattcagcaa
    50281 ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt cactcaaggt
    50341 gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg tgacctggct
    50401 aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca gcgtgtactg
    50461 gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa tgggatcgtc
    50521 tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca tccattctgc
    50581 ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac tgaaacgcat
    50641 tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga
    50701 acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg
    50761 agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc
    50821 gtggtgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca
    50881 taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg cagatccagc
    50941 agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc catcgcgatt
    51001 ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac tggtttcagc
    51061 gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc atcaaatgcc
    51121 actttaccgt tggcatctgg tgtcgccgta aaagtggtat tgctggcaag gcgggtgacc
    51181 tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccac ggaggcatca
    51241 gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc
    51301 ttaccgatag taaagaaatc ttcaccagca tcgccgttag catcaaatcc ggctttgtgt
    51361 tggctgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg
    51421 tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc
    51481 tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg
    51541 tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa
    51601 ccattggcat cgtgatgtta taagtaccgc catcctgaac gctgacttct acgccaacaa
    51661 tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc
    51721 ctgcccccac gcctgtcagg cgcgagattt gatcgttcag gctggcaatt tgtttagcgt
    51781 aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc
    51841 gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg
    51901 cctggcgcgc cgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat
    51961 cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca
    52021 tctgctcata gcgggcggtc aggccgctac tttgcgtctg cgcagcacgt aactggttgg
    52081 taataaacgt atcatactca cgctgcacac cagaaacgta gacgccattg ccaacccagc
    52141 cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg
    52201 caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca
    52261 gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag ccttcaatta
    52321 cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat tatcaatgtt
    52381 catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgga tcatgttggt
    52441 gagtttacgg gcatagtgag gatcggtagc atagcccgcg tcctgtagga cctgcgcccc
    52501 ctgttccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc
    52561 aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg cttttacttt
    52621 cttcgcttcg ccgttttcat attcagtcgt ggtgatttca gtgacttgcc ctttccagtt
    52681 gccagaggct ttgacaccaa acaggttata gcttggctcg ccgttttcgc ggcggatttg
    52741 ccgttgcccc caaccagatt caagcgcagc ctgagcgagg atcaaatgat gtggcacacc
    52801 gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctctactgtc
    52861 acccggcagc gaatcatcgt agttacgtgg tacggccttt tgcaccagct gcgaaagcgt
    52921 ctggttttga taacgcacca cggtttcgag cgggaatttc atcggtgctg ctggcgtgga
    52981 ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag
    53041 acctttgccc gccgtcatct gttgggcaat ttgctggtca tacatactgg tatacagacg
    53101 agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat
    53161 catctgcacg aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc
    53221 ttcgctcgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag
    53281 tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt
    53341 tgcattgatt gcaaaataga catcagatcc atcggcgtag cgcccagcgc attgagcgca
    53401 cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg
    53461 cgtaaatcga tctgcgtttg tggagtaaca acagtctgtc caccaccaaa cggtgtatct
    53521 ggctggctga cattggcctg acgattaact gttactgaga gatttccctg cgctaccgcg
    53581 cagctgtcga gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact
    53641 actttagcgt cc
//
LOCUS       DAJVRP010000016        53212 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVRP010000016 DAJVRP010000000
VERSION     DAJVRP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.016"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(12..323)
                     /locus_tag="OQN12_000939"
     CDS             complement(12..323)
                     /locus_tag="OQN12_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS1653370.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="OQN12_000940"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQN12_000940"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="OQN12_000941"
     CDS             702..1055
                     /locus_tag="OQN12_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS1653371.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="OQN12_000942"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="OQN12_000942"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCS1653372.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="OQN12_000943"
     CDS             complement(2811..3281)
                     /locus_tag="OQN12_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCS1653373.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="OQN12_000944"
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="OQN12_000944"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS1653374.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4120..4338)
                     /gene="hha"
                     /locus_tag="OQN12_000945"
     CDS             complement(4120..4338)
                     /gene="hha"
                     /locus_tag="OQN12_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1653375.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="OQN12_000946"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="OQN12_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1653376.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5284..8433)
                     /locus_tag="OQN12_000947"
     CDS             complement(5284..8433)
                     /locus_tag="OQN12_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1653377.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="OQN12_000948"
     CDS             complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="OQN12_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1653378.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9791..10438
                     /gene="acrR"
                     /locus_tag="OQN12_000949"
     CDS             9791..10438
                     /gene="acrR"
                     /locus_tag="OQN12_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1653379.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10566..13928
                     /gene="mscK"
                     /locus_tag="OQN12_000950"
     CDS             10566..13928
                     /gene="mscK"
                     /locus_tag="OQN12_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1653380.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="OQN12_000951"
     CDS             complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="OQN12_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1653381.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14315..14842)
                     /gene="priC"
                     /locus_tag="OQN12_000952"
     CDS             complement(14315..14842)
                     /gene="priC"
                     /locus_tag="OQN12_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1653382.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14912..15289
                     /locus_tag="OQN12_000953"
     CDS             14912..15289
                     /locus_tag="OQN12_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1653383.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15442..15993
                     /gene="apt"
                     /locus_tag="OQN12_000954"
     CDS             15442..15993
                     /gene="apt"
                     /locus_tag="OQN12_000954"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1653384.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16122..18047
                     /gene="dnaX"
                     /locus_tag="OQN12_000955"
     CDS             16122..18047
                     /gene="dnaX"
                     /locus_tag="OQN12_000955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1653385.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17380..17444
                     /gene="dnaX"
                     /locus_tag="OQN12_000955"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18100..18429
                     /locus_tag="OQN12_000956"
     CDS             18100..18429
                     /locus_tag="OQN12_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1653386.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18429..19034
                     /gene="recR"
                     /locus_tag="OQN12_000957"
     CDS             18429..19034
                     /gene="recR"
                     /locus_tag="OQN12_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1653387.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19144..21018
                     /gene="htpG"
                     /locus_tag="OQN12_000958"
     CDS             19144..21018
                     /gene="htpG"
                     /locus_tag="OQN12_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1653388.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21199..21843
                     /gene="adk"
                     /locus_tag="OQN12_000959"
     CDS             21199..21843
                     /gene="adk"
                     /locus_tag="OQN12_000959"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1653389.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21975..22937
                     /gene="hemH"
                     /locus_tag="OQN12_000960"
     CDS             21975..22937
                     /gene="hemH"
                     /locus_tag="OQN12_000960"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1653390.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22934..23893)
                     /gene="aes"
                     /locus_tag="OQN12_000961"
     CDS             complement(22934..23893)
                     /gene="aes"
                     /locus_tag="OQN12_000961"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCS1653391.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24045..25349
                     /gene="gsk"
                     /locus_tag="OQN12_000962"
     CDS             24045..25349
                     /gene="gsk"
                     /locus_tag="OQN12_000962"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1653392.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="OQN12_000963"
     CDS             complement(25482..27158)
                     /locus_tag="OQN12_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1653393.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="OQN12_000964"
     CDS             complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="OQN12_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1653394.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /gene="ushA"
                     /locus_tag="OQN12_000965"
     CDS             28834..30486
                     /gene="ushA"
                     /locus_tag="OQN12_000965"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1653395.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="OQN12_000966"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="OQN12_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1653396.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="OQN12_000967"
     CDS             complement(31206..32000)
                     /locus_tag="OQN12_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1653397.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="OQN12_000968"
     CDS             32138..32479
                     /locus_tag="OQN12_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1653398.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="OQN12_000969"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="OQN12_000969"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1653399.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="OQN12_000970"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="OQN12_000970"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1653400.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="OQN12_000971"
     CDS             36395..37687
                     /locus_tag="OQN12_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1653401.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38246
                     /gene="cueR"
                     /locus_tag="OQN12_000972"
     CDS             37812..38246
                     /gene="cueR"
                     /locus_tag="OQN12_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1653402.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38220..38678)
                     /locus_tag="OQN12_000973"
     CDS             complement(38220..38678)
                     /locus_tag="OQN12_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1653403.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="OQN12_000974"
     CDS             complement(38675..39592)
                     /locus_tag="OQN12_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1653404.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="OQN12_000975"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="OQN12_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1653405.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="OQN12_000976"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="OQN12_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1653406.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="OQN12_000977"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="OQN12_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1653407.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="OQN12_000978"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="OQN12_000978"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1653408.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="OQN12_000979"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="OQN12_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="OQN12_000980"
     CDS             43551..44237
                     /locus_tag="OQN12_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1653409.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46649
                     /locus_tag="OQN12_000981"
                     /pseudo
     CDS             44234..46649
                     /locus_tag="OQN12_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OQN12_000982"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OQN12_000982"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS1653410.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OQN12_000983"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OQN12_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCS1653411.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="OQN12_000984"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="OQN12_000984"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCS1653412.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="OQN12_000985"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="OQN12_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCS1653413.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="OQN12_000986"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="OQN12_000986"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCS1653414.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53212
                     /locus_tag="OQN12_000987"
     CDS             52939..>53212
                     /locus_tag="OQN12_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCS1653415.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12828 a        13693 c        14020 g        12671 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aatgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ttggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccag gaagggcagt atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
     2161 caataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctag ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgtgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaattagcga gaatttgctg gcgtaatgtg tgctcttccg ccagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc accgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgcg ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccaaccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctggactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg attacatgtt
     5281 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattattgc ggctaaagcg
     5341 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     5401 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     5461 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
     5521 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     5581 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
     5641 cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     5701 accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc agctctcgta
     5761 cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg aaggtgcctg
     5821 gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
     5881 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     5941 cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac gcggcgaacc
     6001 gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat cagcagcacg
     6061 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     6121 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     6181 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     6241 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
     6301 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
     6361 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
     6421 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     6481 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
     6541 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     6601 ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg ttacctcatt
     6661 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     6721 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     6781 gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca gaataccgcc
     6841 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttcaacc agccgaagaa
     6901 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     6961 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
     7021 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     7081 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     7141 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
     7201 aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca ttgttagcgt
     7261 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     7321 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
     7381 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     7441 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     7501 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
     7561 gatccccaga ccggaagcag gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     7621 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct gattcacttt
     7681 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     7741 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
     7801 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
     7861 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
     7921 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
     7981 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     8041 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
     8101 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     8161 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     8221 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     8281 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
     8341 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     8401 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     8461 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     8521 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     8581 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
     8641 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
     8701 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     8761 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     8821 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     8881 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     8941 gtttcagcgt gccattcgcc agttcctgtt tcaggcgaag gaagtcgttg ctggactggg
     9001 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
     9061 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
     9121 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
     9181 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc ttactgatgt
     9241 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     9301 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
     9361 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
     9421 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
     9481 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     9541 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     9601 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     9661 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     9721 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     9781 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     9841 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
     9901 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     9961 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    10021 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    10081 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    10141 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    10201 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    10261 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    10321 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    10381 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta acgaataacc
    10441 ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct ggttcactgc
    10501 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    10561 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    10621 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    10681 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    10741 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    10801 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    10861 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    10921 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    10981 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    11041 tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc
    11101 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    11161 gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt gaccagcagc
    11221 gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa cgtgattacg
    11281 tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa gcggtaaaca
    11341 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    11401 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    11461 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    11521 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    11581 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    11641 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    11701 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    11761 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    11821 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    11881 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    11941 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    12001 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    12061 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    12121 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    12181 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    12241 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    12301 tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    12361 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    12421 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    12481 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    12541 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    12601 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc attaccgtgc
    12661 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    12721 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    12781 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    12841 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    12901 ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    12961 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc gtgttcagct
    13021 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    13081 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    13141 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgcc
    13201 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    13261 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    13321 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    13381 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    13441 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    13501 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    13561 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    13621 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    13681 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    13741 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    13801 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    13861 agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg acaccagcgg
    13921 ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    13981 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    14041 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    14101 ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    14161 gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca gcacggtact
    14221 ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    14281 aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc gctagcctgt
    14341 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccaatt
    14401 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    14461 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    14521 agcgggaaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gaggcttcac
    14581 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    14641 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg tcgagacaag
    14701 actgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg
    14761 cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    14821 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    14881 gtagccacta tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    14941 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    15001 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    15061 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    15121 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    15181 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    15241 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    15301 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    15361 cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    15421 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    15481 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    15541 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    15601 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    15661 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    15721 gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    15781 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    15841 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    15901 ttcattatca acctgtttga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    15961 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    16021 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    16081 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggttttag
    16141 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    16201 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    16261 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    16321 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    16381 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    16441 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    16501 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    16561 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    16621 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    16681 ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    16741 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    16801 gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
    16861 atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac
    16921 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    16981 gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    17041 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    17101 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    17161 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg
    17221 agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa
    17281 ccttggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    17341 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    17401 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    17461 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    17521 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    17581 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    17641 tggcggtgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca caggtgagcc
    17701 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    17761 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggtgcac
    17821 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    17881 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    17941 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    18001 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    18061 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    18121 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    18181 atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    18241 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    18301 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    18361 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    18421 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    18481 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    18541 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    18601 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    18661 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    18721 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    18781 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    18841 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    18901 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    18961 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc gggcgtcata
    19021 agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa ttattctccc
    19081 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    19141 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    19201 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    19261 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    19321 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    19381 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    19441 tccggtacca aatcattcct cgaatccctg ggttctgacc aagcgaaaga cagccagctg
    19501 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    19561 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    19621 ggtgaatata ccgttgccga tatcaccaaa gaagatcgtg gtactgaaat caccctgcat
    19681 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    19741 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
    19801 accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    19861 gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga ctttaacgat
    19921 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    19981 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    20041 tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa ctatctgcgc
    20101 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    20161 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    20221 ctagaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    20281 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    20341 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    20401 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    20461 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    20521 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    20581 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    20641 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa agctctgctc
    20701 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    20761 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    20821 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    20881 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    20941 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaat
    21001 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    21061 tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    21121 tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa atcgacacat
    21181 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    21241 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    21301 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    21361 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    21421 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    21481 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    21541 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    21601 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    21661 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    21721 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    21781 aaagttgacg gtaccaagca ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    21841 taatcaagcc gcagcagatc tgctttcggg caggtctgct tatccacctc aggcaatacc
    21901 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    21961 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    22021 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    22081 gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc
    22141 tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac ggctcgccgc
    22201 tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgccag
    22261 tggcgctggg catgagctac ggctcgccat cactggaaag ctccgtcgat gaactcctgg
    22321 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    22381 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    22441 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    22501 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    22561 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    22621 gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc tttcagtcgc
    22681 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    22741 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    22801 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    22861 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    22921 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    22981 cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    23041 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    23101 gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    23161 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    23221 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    23281 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    23341 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    23401 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    23461 aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa
    23521 gggtgtaatt aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    23581 gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga gctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gccaggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggctgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggttcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgct
    28261 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacctggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gacgacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttacttacta cggtaattga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atacggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgctg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca ctggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 ctgcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aatcgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggctatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 tatcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggggat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 ggggcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtcttgcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggagttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaatgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgatgttc gccatgcttg
    46441 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
    46501 cgaaagtgtt tgatctcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    46561 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta
    46621 aggcgctgtt caggcagttt gtggggtgat gacaggtgtt agattgcctg atgcgctacg
    46681 cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat
    46741 aaggcgttta cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca
    46801 actgtcatgt cggttttttt aaaggccgaa ggaggtaaac cttcggcctt agctacgttt
    46861 cctcagttat tcaataaact gacggatcag ttccagcagc gcgattagcg ccttaagaac
    46921 gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag aaagacaatc
    46981 acagcttacc cttacgttgc ctataactat gattttcttc atgtgctaga tgaggactgt
    47041 ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag gacttaaaag
    47101 tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc gggattgtat
    47161 cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt gctagatgag
    47221 gactgtttaa tttctgcccg gaaccaccaa gtcttgggcg gaaaaaataa aaaaaccgac
    47281 tgtcatgtcg gttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattgatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc actcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaattaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc ctacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct gggggtttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat tc
//
LOCUS       DAJVRP010000017        51899 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVRP010000017 DAJVRP010000000
VERSION     DAJVRP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.017"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="OQN12_000988"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="OQN12_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1653416.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="OQN12_000989"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="OQN12_000989"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1653417.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="OQN12_000990"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="OQN12_000990"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1653418.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="OQN12_000991"
     CDS             complement(5906..6949)
                     /locus_tag="OQN12_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1653419.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="OQN12_000992"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="OQN12_000992"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1653420.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="OQN12_000993"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="OQN12_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS1653421.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="OQN12_000994"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="OQN12_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="OQN12_000995"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="OQN12_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCS1653422.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="OQN12_000996"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="OQN12_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1653423.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="OQN12_000997"
     CDS             15474..15830
                     /locus_tag="OQN12_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1653424.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="OQN12_000998"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="OQN12_000998"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1653425.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="OQN12_000999"
     CDS             16989..17189
                     /locus_tag="OQN12_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1653426.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="OQN12_001000"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="OQN12_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1653427.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="OQN12_001001"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="OQN12_001001"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1653428.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="OQN12_001002"
     CDS             complement(20101..20964)
                     /locus_tag="OQN12_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1653429.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="OQN12_001003"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="OQN12_001003"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1653430.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="OQN12_001004"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="OQN12_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1653431.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="OQN12_001005"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="OQN12_001005"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1653432.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="OQN12_001006"
     CDS             24133..24705
                     /locus_tag="OQN12_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1653433.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     TFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="OQN12_001007"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="OQN12_001007"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1653434.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="OQN12_001008"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="OQN12_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1653435.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="OQN12_001009"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="OQN12_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="OQN12_001010"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="OQN12_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1653436.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="OQN12_001011"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="OQN12_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCS1653437.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="OQN12_001012"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="OQN12_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1653438.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="OQN12_001013"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="OQN12_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1653439.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="OQN12_001014"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="OQN12_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="OQN12_001015"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="OQN12_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1653440.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="OQN12_001016"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="OQN12_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1653441.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="OQN12_001017"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="OQN12_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1653442.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="OQN12_001018"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="OQN12_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCS1653443.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="OQN12_001019"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="OQN12_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="OQN12_001020"
     CDS             complement(39099..40160)
                     /locus_tag="OQN12_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1653444.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="OQN12_001021"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="OQN12_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1653445.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="OQN12_001022"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="OQN12_001022"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1653446.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="OQN12_001023"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="OQN12_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1653447.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="OQN12_001024"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="OQN12_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1653448.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="OQN12_001025"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="OQN12_001025"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1653449.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="OQN12_001026"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="OQN12_001026"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1653450.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="OQN12_001027"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="OQN12_001027"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1653451.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="OQN12_001028"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="OQN12_001028"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1653452.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="OQN12_001029"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="OQN12_001029"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1653453.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>51899)
                     /gene="pdeF"
                     /locus_tag="OQN12_001030"
     CDS             complement(50969..>51899)
                     /gene="pdeF"
                     /locus_tag="OQN12_001030"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1653454.1"
                     /translation="PQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRCG
                     AMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIG
                     PGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVE
                     VSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYA
                     RLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAV
                     LSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12111 a        13694 c        13477 g        12617 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacgactc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 cagcatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aaacgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggt
//
LOCUS       DAJVRP010000018        49530 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVRP010000018 DAJVRP010000000
VERSION     DAJVRP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.018"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OQN12_001031"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OQN12_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1653455.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="OQN12_001032"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="OQN12_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1653456.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="OQN12_001033"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="OQN12_001033"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1653457.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="OQN12_001034"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="OQN12_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1653458.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="OQN12_001035"
     CDS             5718..6659
                     /locus_tag="OQN12_001035"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1653459.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="OQN12_001036"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="OQN12_001036"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1653460.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="OQN12_001037"
     CDS             7679..8929
                     /locus_tag="OQN12_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1653461.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OQN12_001038"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OQN12_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="OQN12_001039"
                     /pseudo
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="OQN12_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10866..12116
                     /locus_tag="OQN12_001040"
     CDS             10866..12116
                     /locus_tag="OQN12_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1653462.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="OQN12_001041"
     CDS             complement(12172..13260)
                     /locus_tag="OQN12_001041"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1653463.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OQN12_001042"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OQN12_001042"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS1653464.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="OQN12_001043"
     CDS             complement(14194..15243)
                     /locus_tag="OQN12_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1653465.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="OQN12_001044"
     CDS             15342..16223
                     /locus_tag="OQN12_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1653466.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="OQN12_001045"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="OQN12_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1653467.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="OQN12_001046"
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="OQN12_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCS1653468.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OQN12_001047"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OQN12_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="OQN12_001048"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="OQN12_001048"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1653469.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /locus_tag="OQN12_001049"
     CDS             21991..23067
                     /locus_tag="OQN12_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1653470.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="OQN12_001050"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="OQN12_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCS1653471.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="OQN12_001051"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="OQN12_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1653472.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="OQN12_001052"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="OQN12_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1653473.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="OQN12_001053"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="OQN12_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1653474.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OQN12_001054"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OQN12_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OQN12_001055"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OQN12_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCS1653475.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="OQN12_001056"
     CDS             complement(30809..31048)
                     /locus_tag="OQN12_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1653476.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OQN12_001057"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OQN12_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1653477.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OQN12_001058"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OQN12_001058"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1653478.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="OQN12_001059"
     CDS             complement(32934..33629)
                     /locus_tag="OQN12_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1653479.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="OQN12_001060"
     CDS             complement(33626..34024)
                     /locus_tag="OQN12_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1653480.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="OQN12_001061"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="OQN12_001061"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS1653481.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OQN12_001062"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OQN12_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS1653482.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="OQN12_001063"
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="OQN12_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCS1653483.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37377..38138
                     /locus_tag="OQN12_001064"
                     /pseudo
     CDS             37377..38138
                     /locus_tag="OQN12_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="OQN12_001065"
     CDS             complement(38255..38833)
                     /locus_tag="OQN12_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1653484.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="OQN12_001066"
     CDS             39003..39590
                     /locus_tag="OQN12_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1653485.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="OQN12_001067"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="OQN12_001067"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1653486.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..42449)
                     /gene="dld"
                     /locus_tag="OQN12_001068"
     CDS             complement(40734..42449)
                     /gene="dld"
                     /locus_tag="OQN12_001068"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1653487.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42645..44942
                     /gene="bglX"
                     /locus_tag="OQN12_001069"
     CDS             42645..44942
                     /gene="bglX"
                     /locus_tag="OQN12_001069"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1653488.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45194..46111
                     /gene="osmF"
                     /locus_tag="OQN12_001070"
     CDS             45194..46111
                     /gene="osmF"
                     /locus_tag="OQN12_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS1653489.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46118..47275
                     /gene="yehY"
                     /locus_tag="OQN12_001071"
     CDS             46118..47275
                     /gene="yehY"
                     /locus_tag="OQN12_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS1653490.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47268..48194
                     /gene="yehX"
                     /locus_tag="OQN12_001072"
     CDS             47268..48194
                     /gene="yehX"
                     /locus_tag="OQN12_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1653491.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48199..48930
                     /gene="yehW"
                     /locus_tag="OQN12_001073"
     CDS             48199..48930
                     /gene="yehW"
                     /locus_tag="OQN12_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1653492.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48911..49018)
                     /locus_tag="OQN12_001074"
     CDS             complement(48911..49018)
                     /locus_tag="OQN12_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1653493.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49078..49530)
                     /locus_tag="OQN12_001075"
                     /pseudo
     CDS             complement(49078..49530)
                     /locus_tag="OQN12_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11955 a        12199 c        13309 g        12067 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ctagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgta gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gcggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagatc cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cacagaatgt ctgaattaaa
     6661 aatctcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgaccg gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcga tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgctaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttattcat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcagata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtatg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagcag gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggc gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaatatt tggattgata
    18001 attgttatcg tttgcattat cgttacgaca caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctggac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggtgggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggcggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agactctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcgggtct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gattgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atctgcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggatg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt gttactggca ccgatggagg gagtgcttga ctctcaggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc gactgcgacg
    34921 cagtgatgat tggtcgcggg gcgttcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagtcctttg ccgttatcca taaacgatta agataaatcg gaagatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat tcagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttactg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagagc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattata cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc aatccccaga
    41881 tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga catttcggta
    41941 tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc gccgatgacc
    42001 gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag cggtttgagg
    42061 gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc gcccttgccg
    42121 agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg atcatagtcg
    42181 ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat cagaataatt
    42241 ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga gccagggaaa
    42301 acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg ggccgttttt
    42361 gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt caaaaaggct
    42421 ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg aaaaaatatc
    42481 acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga aaattcagac
    42541 aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac ggtttttgca
    42601 ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt
    42661 cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat ttattcggta
    42721 accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatttgctc aagaaaatga
    42781 cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat aatccgaaag
    42841 aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac accgtaaccc
    42901 gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg aaaattcctc
    42961 ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt agcctcggtc
    43021 tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct gcttatgaag
    43081 cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg cgcgatccgc
    43141 gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca ataatgggta
    43201 aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg gtgatgacca
    43261 gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg
    43321 atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg gggctggacg
    43381 caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca gccacctccg
    43441 attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc atcaccgttt
    43501 ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac ccggaagatg
    43561 cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag tactactcga
    43621 agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg gacgatgctg
    43681 cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca tacagccatt
    43741 tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg caccgtaaag
    43801 aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac
    43861 cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt aaacgtgacg
    43921 tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta ctgaccggga
    43981 ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg aacgttacca
    44041 gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc
    44101 gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct gatgtggtgg
    44161 tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg accgatatca
    44221 ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt aaaccgctgg
    44281 tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag caggctgatg
    44341 cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc gatgtattgt
    44401 ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct gtcgggcaga
    44461 tcccggtgta ctacagccat ctgaacactg gtcgtccgta taatgccgac aagccgaaca
    44521 aatacacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc ggctatggtc
    44581 tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc atgaagcgtg
    44641 acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag ggggccacgg
    44701 tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg aaacagctga
    44761 aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc ttcccgatcg
    44821 atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag cctggcaagt
    44881 tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt gagttgctgt
    44941 aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat cttaacttgc
    45001 tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc aggaaacgcc
    45061 ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt ccgctcaggc
    45121 taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
    45181 tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta tgttggcagc
    45241 cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa tcgataccga
    45301 aggcgcgctg ctcggcaaca tcatctttca ggtactcgaa agccacggcg taccgacggt
    45361 gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt ccggtgaact
    45421 ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg aaaacgatgc
    45481 agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg cggagcacaa
    45541 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    45601 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgcaaga
    45661 gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg atgcgttacc
    45721 cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt cactggctgg
    45781 tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg ttaatgccgc
    45841 aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct taagcgatcc
    45901 gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg tgttgaagga
    45961 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    46021 gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag tggctgccga
    46081 ctacctgaaa caaaaagggt gggcgaagta atttcccgtg acttatctac gtattaatcc
    46141 tgttctggcg ctgctgttgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    46201 cgcgcctaat cgtttagtct cgggtgaagg gcttcatctc tggcaactgt ggccgcaaac
    46261 gatctggatg ctggtgggcg ttggttgcgc ctggctgaca gcctgttttg tccccgctaa
    46321 aaaaggcagc atttttgcac tcattctggc gcaattcgtc ttcgtattgt tggtgtgggg
    46381 agctggaaag gcggcgaccc aactggcgca aaatggcagt gcgctggcgc gtaccagcct
    46441 cggcagaggt ttctggctgg ctgcggcgct ggcattgctg gcctgtagcg atgccatccg
    46501 ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    46561 gctgtggttg ctgtactccg gcacgcttaa cgatctctca ctaatgaaag aatacgccaa
    46621 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    46681 gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg tgctactttt ccactgcgcg
    46741 gcagggggca attttttctc tgctcaatgt cattcagacc gtgccttcgg tggcgctctt
    46801 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tggggaagct
    46861 cggcatagca ggaaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    46921 gccgctggtg cgaggcgtgg tagtcggttt gaaccagatc ccgcgcgatg tgctggagag
    46981 cgccagagcg atggggatga gcggggcgca gcgtttcctg catgttcagt taccgctggc
    47041 gttactggta tttctgcgca gcctgcgggt ggtgatggtg caaactgtag gtatggcggt
    47101 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    47161 cagcgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgcttac
    47221 cgacgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    47281 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    47341 aggaagggag tttttcggtg ctgattggca catctggctc cggcaaatcc accaccctga
    47401 aaatgattaa ccgcctggtg gagcatgaca gcggagtgat ccgctttgcc ggagaagaaa
    47461 ttcgctcgct gccagtactg gagttgcgcc gccggatggg ctatgccatt caatctattg
    47521 gcctgttccc ccactggagc gtggcgcaaa acatcgctac cgtgccgcaa ttacaaaaat
    47581 ggtcgcgggc gcggattgac gatcgtatcg acgaattaat ggcgctactg gggctggagt
    47641 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    47701 tggcgcgtgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcac
    47761 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcattcac cgtttgctgg
    47821 ggcgcactat tgtgctggtc actcatgata ttgatgaggc gctacggctg gcagaacatc
    47881 tggtattgat ggatcacggt gaagtggtgc agcaggggaa tccgctgacg atgctgactc
    47941 gtccggcgaa tgattttgtc cgccagtttt ttggacgtag tgaactgggg gtgcacctgc
    48001 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcac
    48061 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgcgagg
    48121 tgctgccggt ggtgaacacg cagggccagc cttgcggcac gctgcatttt caggatctgc
    48181 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctctt
    48241 tttgtggcgc tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    48301 caactgccac gacccgttta tcagcaagaa agttttgcag ctctggcact ggctcatttc
    48361 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcacaggtgc cggaattgct
    48421 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    48481 cagacttttc cgcctgtcgc agtgctggcg attgccgttc cggtgatcgg ctttggtctg
    48541 caaccagcga ttatcgcctt gatcctttac ggtgtgctgc ccatcctgca ggtgacactt
    48601 gccgggctgg gagcgattga tcccagcgtg acagaagttg cgaaaggtat gggaatgagt
    48661 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggc
    48721 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    48781 agtacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    48841 cagggggcgt tactggtggc actggcggcg atcatcgcag accgcctgtt tgaaaggctg
    48901 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    48961 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    49021 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    49081 aataccgagt gggaaaatat catggccttg ttcttgccaa ctggtgagtt gctgctgttg
    49141 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga atagttcagg
    49201 gcgtagttga ttgagcgagt gggcgagcga ggacatcaat gcgccatcct tgttgactgg
    49261 caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca accaccagcg
    49321 catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag ttgatcagta
    49381 cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga agccgtcggc
    49441 gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga ccgcgctctt
    49501 tgatccacgt tcgcaggcta tgtagattgc
//
LOCUS       DAJVRP010000019        48548 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVRP010000019 DAJVRP010000000
VERSION     DAJVRP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.019"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..1222
                     /locus_tag="OQN12_001076"
     CDS             <1..1222
                     /locus_tag="OQN12_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1653494.1"
                     /translation="ELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHP
                     VITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMII
                     PEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIA
                     LLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYV
                     NTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNF
                     KVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETV
                     LNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKY
                     LLAQFGVDIGEAAQ"
     gene            1219..2184
                     /gene="yggF"
                     /locus_tag="OQN12_001077"
     CDS             1219..2184
                     /gene="yggF"
                     /locus_tag="OQN12_001077"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1653495.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2206..2715
                     /gene="fumE"
                     /locus_tag="OQN12_001078"
     CDS             2206..2715
                     /gene="fumE"
                     /locus_tag="OQN12_001078"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCS1653496.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2712..3425
                     /locus_tag="OQN12_001079"
     CDS             2712..3425
                     /locus_tag="OQN12_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1653497.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3397..4079)
                     /locus_tag="OQN12_001080"
                     /pseudo
     CDS             complement(3397..4079)
                     /locus_tag="OQN12_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4073..4750)
                     /locus_tag="OQN12_001081"
     CDS             complement(4073..4750)
                     /locus_tag="OQN12_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1653498.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4738..5121)
                     /locus_tag="OQN12_001082"
     CDS             complement(4738..5121)
                     /locus_tag="OQN12_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1653499.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5137..5445)
                     /locus_tag="OQN12_001083"
     CDS             complement(5137..5445)
                     /locus_tag="OQN12_001083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653500.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5446..6024)
                     /locus_tag="OQN12_001084"
     CDS             complement(5446..6024)
                     /locus_tag="OQN12_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1653501.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6047..6478)
                     /locus_tag="OQN12_001085"
                     /pseudo
     CDS             complement(6047..6478)
                     /locus_tag="OQN12_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6850..7869
                     /gene="epd"
                     /locus_tag="OQN12_001086"
     CDS             6850..7869
                     /gene="epd"
                     /locus_tag="OQN12_001086"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1653502.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7919..9082
                     /gene="pgk"
                     /locus_tag="OQN12_001087"
     CDS             7919..9082
                     /gene="pgk"
                     /locus_tag="OQN12_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1653503.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9297..10376
                     /gene="fbaA"
                     /locus_tag="OQN12_001088"
     CDS             9297..10376
                     /gene="fbaA"
                     /locus_tag="OQN12_001088"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1653504.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10567..11427
                     /gene="mscS"
                     /locus_tag="OQN12_001089"
     CDS             10567..11427
                     /gene="mscS"
                     /locus_tag="OQN12_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1653505.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11566..12201
                     /gene="argO"
                     /locus_tag="OQN12_001090"
     CDS             11566..12201
                     /gene="argO"
                     /locus_tag="OQN12_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1653506.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12294..13034
                     /locus_tag="OQN12_001091"
     CDS             12294..13034
                     /locus_tag="OQN12_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1653507.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13201..14151
                     /locus_tag="OQN12_001092"
     CDS             13201..14151
                     /locus_tag="OQN12_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1653508.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14093..15571)
                     /gene="scpC"
                     /locus_tag="OQN12_001093"
     CDS             complement(14093..15571)
                     /gene="scpC"
                     /locus_tag="OQN12_001093"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCS1653509.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15595..16371)
                     /gene="scpB"
                     /locus_tag="OQN12_001094"
     CDS             complement(15595..16371)
                     /gene="scpB"
                     /locus_tag="OQN12_001094"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1653510.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16382..17377)
                     /gene="meaB"
                     /locus_tag="OQN12_001095"
     CDS             complement(16382..17377)
                     /gene="meaB"
                     /locus_tag="OQN12_001095"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1653511.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17370..19514)
                     /gene="scpA"
                     /locus_tag="OQN12_001096"
     CDS             complement(17370..19514)
                     /gene="scpA"
                     /locus_tag="OQN12_001096"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCS1653512.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19718..20611)
                     /gene="argP"
                     /locus_tag="OQN12_001097"
     CDS             complement(19718..20611)
                     /gene="argP"
                     /locus_tag="OQN12_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1653513.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20994..21653
                     /gene="rpiA"
                     /locus_tag="OQN12_001098"
     CDS             20994..21653
                     /gene="rpiA"
                     /locus_tag="OQN12_001098"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1653514.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21909..23141
                     /gene="serA"
                     /locus_tag="OQN12_001099"
     CDS             21909..23141
                     /gene="serA"
                     /locus_tag="OQN12_001099"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1653515.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23370..23429
                     /gene="ibsC"
                     /locus_tag="OQN12_001100"
     CDS             23370..23429
                     /gene="ibsC"
                     /locus_tag="OQN12_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1653516.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23762..23821
                     /locus_tag="OQN12_001101"
     CDS             23762..23821
                     /locus_tag="OQN12_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1653517.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23922..24524)
                     /locus_tag="OQN12_001102"
     CDS             complement(23922..24524)
                     /locus_tag="OQN12_001102"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1653518.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24545..24728)
                     /gene="ssrS"
                     /locus_tag="OQN12_001103"
     ncRNA           complement(24545..24728)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQN12_001103"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24770..25099)
                     /gene="zapA"
                     /locus_tag="OQN12_001104"
     CDS             complement(24770..25099)
                     /gene="zapA"
                     /locus_tag="OQN12_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1653519.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25267..25845
                     /gene="ygfB"
                     /locus_tag="OQN12_001105"
     CDS             25267..25845
                     /gene="ygfB"
                     /locus_tag="OQN12_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1653520.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25871..27196
                     /gene="pepP"
                     /locus_tag="OQN12_001106"
     CDS             25871..27196
                     /gene="pepP"
                     /locus_tag="OQN12_001106"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1653521.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27193..28371
                     /gene="ubiH"
                     /locus_tag="OQN12_001107"
                     /gene_synonym="visB"
     CDS             27193..28371
                     /gene="ubiH"
                     /locus_tag="OQN12_001107"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1653522.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28394..29596
                     /gene="ubiI"
                     /locus_tag="OQN12_001108"
                     /gene_synonym="visC"
     CDS             28394..29596
                     /gene="ubiI"
                     /locus_tag="OQN12_001108"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1653523.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29670..29753)
                     /locus_tag="OQN12_001109"
     CDS             complement(29670..29753)
                     /locus_tag="OQN12_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653524.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30044..31138
                     /gene="gcvT"
                     /locus_tag="OQN12_001110"
     CDS             30044..31138
                     /gene="gcvT"
                     /locus_tag="OQN12_001110"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1653525.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31162..31551
                     /gene="gcvH"
                     /locus_tag="OQN12_001111"
     CDS             31162..31551
                     /gene="gcvH"
                     /locus_tag="OQN12_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1653526.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31670..34543
                     /gene="gcvP"
                     /locus_tag="OQN12_001112"
     CDS             31670..34543
                     /gene="gcvP"
                     /locus_tag="OQN12_001112"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1653527.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34810..35552
                     /locus_tag="OQN12_001113"
                     /pseudo
     CDS             34810..35552
                     /locus_tag="OQN12_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35609..37042)
                     /gene="bglA"
                     /locus_tag="OQN12_001114"
     CDS             complement(35609..37042)
                     /gene="bglA"
                     /locus_tag="OQN12_001114"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS1653528.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37087..37397
                     /locus_tag="OQN12_001115"
                     /pseudo
     CDS             37087..37397
                     /locus_tag="OQN12_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37561..38220
                     /locus_tag="OQN12_001116"
     CDS             37561..38220
                     /locus_tag="OQN12_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1653529.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38416..39396)
                     /gene="ygfZ"
                     /locus_tag="OQN12_001117"
     CDS             complement(38416..39396)
                     /gene="ygfZ"
                     /locus_tag="OQN12_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1653530.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39639..39905
                     /gene="sdhE"
                     /locus_tag="OQN12_001118"
     CDS             39639..39905
                     /gene="sdhE"
                     /locus_tag="OQN12_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1653531.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39886..40293
                     /locus_tag="OQN12_001119"
     CDS             39886..40293
                     /locus_tag="OQN12_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCS1653532.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40333..40854)
                     /gene="fldB"
                     /locus_tag="OQN12_001120"
     CDS             complement(40333..40854)
                     /gene="fldB"
                     /locus_tag="OQN12_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1653533.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40966..41862
                     /gene="xerD"
                     /locus_tag="OQN12_001121"
     CDS             40966..41862
                     /gene="xerD"
                     /locus_tag="OQN12_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1653534.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41887..42597
                     /gene="dsbC"
                     /locus_tag="OQN12_001122"
     CDS             41887..42597
                     /gene="dsbC"
                     /locus_tag="OQN12_001122"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1653535.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42603..44336
                     /gene="recJ"
                     /locus_tag="OQN12_001123"
     CDS             42603..44336
                     /gene="recJ"
                     /locus_tag="OQN12_001123"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1653536.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44427..45525
                     /gene="prfB"
                     /locus_tag="OQN12_001124"
     CDS             join(44427..44501,44503..45525)
                     /gene="prfB"
                     /locus_tag="OQN12_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1653537.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45535..47052
                     /gene="lysS"
                     /locus_tag="OQN12_001125"
     CDS             45535..47052
                     /gene="lysS"
                     /locus_tag="OQN12_001125"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1653538.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47095..47643)
                     /gene="idi"
                     /locus_tag="OQN12_001126"
     CDS             complement(47095..47643)
                     /gene="idi"
                     /locus_tag="OQN12_001126"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1653539.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47698..47769
                     /locus_tag="OQN12_001127"
     CDS             47698..47769
                     /locus_tag="OQN12_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653540.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47766..47891)
                     /locus_tag="OQN12_001128"
     CDS             complement(47766..47891)
                     /locus_tag="OQN12_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653541.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47893..>48548)
                     /gene="uacT"
                     /locus_tag="OQN12_001129"
     CDS             complement(47893..>48548)
                     /gene="uacT"
                     /locus_tag="OQN12_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1653542.1"
                     /translation="IVFIESMGMFLALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFN
                     SFPHTSFSQNVGLVSVMRVHSRWVCISSGIILILFGMVPKMAVLVASIPQFVLGGAGL
                     VMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLH
                     SGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11645 a        12176 c        13110 g        11617 t
ORIGIN      
        1 tgaactgcca gaaattaccg ataatgaact gttagtgagt gtaatttctg acagcgtctg
       61 tttatcgacc tggaaagcgg cgttactcgg tagtgaacat aaacgcgtac ccgacgattt
      121 agaaaatcat ccggtcatta ccgggcatga atgtgccggg gttattgtcg aagtgggtaa
      181 aaatctcact ggcaaatata aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt
      241 accaagcgga tattcagcgg gctacagcta cgaatatttt ggcggcaatg ccacttatat
      301 gattattccc gaaatcgcca ttaatttggg ctgcgtatta ccgtatcacg gctcttattt
      361 tgctgcggcg tcgctggcag agcctatgtg ctgcattatt ggtgcttatc atgccaatta
      421 tcacaccacg caatatgttt atgagcatcg catgggcgtc aaacctggcg gcaatattgc
      481 actgctggcg tgtgcgggtc cgatgggcat tggcgctatc gattacgcca ttaacggtgg
      541 catacaaccg tcacgggtgg tggtggtcga tatcgacgac aaacgcctgg cgcaggtgca
      601 gaagctgctg ccggtggatc tggcggccag taaaggcatt gagctagtgt atgtgaatac
      661 caaagggatg agcgatccta tccagatgct gcgggcgctg acaggagatg tcgggttcga
      721 tgacattttt gtttatgcgg cggtgcctgc tgtcgttgag atggctgacg aattactggc
      781 ggaagatggc tgtctgaact tctttgccgg gctgacggat aaaaacttca aagtgccgtt
      841 taatttctac aacgtccatt acaacagcac gcacgtagtc ggtacatccg gcggttcaac
      901 ggacgacatg aaagaggcga ttgcccttag cgccacagga cagttacagc cgtcgtttat
      961 ggtgacccat attggtggcc tggatgcggt gccagaaacc gtgctcaatc tgccggatat
     1021 ccctggcggt aaaaaactca tttataacgg cgtgaccatg ccgctcactg ccattgccga
     1081 ttttgccgaa aaaggcaaaa ccgatccgct gtttaaagag ttggcgcggc tggttgagga
     1141 aacgcacggc atctggaatg aacaggccga gaaatatctg ctggcacaat ttggcgttga
     1201 tatcggggag gccgcgcaat gatgtccctg gcgtggccat tatttcgcgt tacggaacag
     1261 gcagcgttgg ctgcctggcc gcacaccgga tgtggcgaca aaaataaaat tgatggcctg
     1321 gcggtcaccg cgatgcgcca ggcattaaac gacgtcgctt ttcgtggacg agtggttatc
     1381 ggcgaaggag aaattgacca tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc
     1441 gatgggccag aagtcgatat cgcggttgat cccattgaag gcacgcggat ggtggcgatg
     1501 gggcagagca atgcgctggc ggtcatggct ttcgctcctc gagatagcct gttacatgcg
     1561 cccgatatgt atatgaaaaa gctggttgtt aatcgactgg ctgccggggc gattgatctg
     1621 tctttgcctc tggcggacaa cttgcgtaac gtggcgcggg cgttaggtaa accgctggac
     1681 aagctgcgca tggttacgct ggataaaccg cgtctgagtg ccgcgattga agaagcaaca
     1741 cagctgggcg tgaaggtttt tgccctgccg gatggcgatg tcgccgccag cgtgctgacc
     1801 tgctggcagg ataatcctta cgatgtgatg tacaccatcg gcggtgcgcc agaaggcgtg
     1861 atttctgcct gtgccgttaa agcgttaggc ggggatatgc aggccgagct gattgacttt
     1921 tgtcaggcta aaggggatta cacggaaaat cggcagattg cagagcagga gcgcaagcgt
     1981 tgtaaggcaa tgggcgttga cgttaaccgt gtgtactcgc tcgatgaact ggtgaggggg
     2041 aacgatatcc tctttagcgc tacgggcgtg acgggcggcg agttggtgaa cggtatccaa
     2101 cagacggcga atggggtgcg gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt
     2161 aatataatag actccctgca ttgatggtga tctatcggaa gaccaatggc gacgctgaca
     2221 gaagatgatg tgcttgagca actggatgca caggacaatt tattttcatt tatgaaaact
     2281 gcgcattcta ttttgctcca ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac
     2341 gatgaagaga tcgtcgaata tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac
     2401 gatattgatg ttgcgctgcg tttgatttat gcgctgggga aaatggataa atggctgtat
     2461 gccgatatca cgcacttttc ccagtactgg cattacctga acgaacagga tgaaacgccc
     2521 ggatttgccg atgatataac ctgggatttt atcagcaatg tcaacagtat aactcgcaat
     2581 gcgacgctct acgatgcgtt aaaagcgatg aagttcgccg atttcgccgt ctggtcagag
     2641 gcgcgtttta gcggaatggt caaaacggcg ctgacgctgg cagtaacgac caccttaaag
     2701 gaattaacgc cgtgaaaatt gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc
     2761 atgatgaaga gatagaacgc gtgcataaac tgttgctgcg tatgctggcg gccttacaga
     2821 cggtaaatcc gcagcggcgt acggtggttt ttctttgcgc tccgccgggg acgggtaaat
     2881 ctaccctaac caccttctgg gaatatctcg cgcaacaaga cccggagctg cccgctattc
     2941 aggcgctccc gatggatggt tttcaccatt acaatagctg gctggacgcg catcaattgc
     3001 gccccttcaa aggcgcacca gagacatttg acgttgcgaa actggcggaa aatctgcgcc
     3061 aggtggtgga aggggattgt acgtggccgc agtacgatcg acaaaagcat gatcctgttg
     3121 aagatgcgtt gcacgttacc gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg
     3181 acgacgagaa gtggtgccag cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc
     3241 cagctgctgc gttgcgggag cgtctggtgg ggcgcaagct ggcgggaggg gtatcgctgg
     3301 cagatgcaga agccttttat gaccgtaccg acgggccgaa tgttcgccgg gtgctggaag
     3361 agagtctgcc tgcaaatttg accttaatga tgaccgctac gggtgaatat cgtctggtgg
     3421 attaacgttc ctgattacac catcttccag ccgcaccaca cgagagaaat gacgccgggt
     3481 aaatgcggcg tcatgactga ttgctaccac gctcgtgcct cgctggcggc acttctccag
     3541 ccagctttca aacacgctca accaattttc gtcaaaatcc cgactcggct cgtccagcag
     3601 taatagtggc ggagagagtg cttccaggca ggcgacggcg accatccgtc gctgcgcact
     3661 atgcagatcc aacgggtgcg agttggcagt gtctgctaac tgacaacatt gcagggcagc
     3721 atgggtacgt tgagtgattt catccgccgg gcatttttgc agtttcaaac caaatgcgat
     3781 ctcgtcagcg acggtgctgt gaaaaagttg gttttccgct tcctgaaaca gaacagtacg
     3841 ccaacctttg ccgcgcgctg gcggtttttc aggtttttca tcgcctgttg ttgcagcata
     3901 accgtaccgg ctgtgggcgt aagaagacct gccattactc gtaacagcgt cgatttccct
     3961 gcgccattgt caccggtcag cgccaaccat tcaccttgtt tgagttgcag agaaatgtcg
     4021 caaagacaat ccgttgccgc gccaggccag cgataagata tcttatttaa cgttaacata
     4081 gtggctggag cgctccgttt tgcaattgcc acgcttgctg gcagtagttc aggaaaggtg
     4141 tgcgatgacg ttcaaaaaga ataatcagcg aaccacgttc aaacgcccag tgttgtaagc
     4201 gctgcaacag catttcgctg gcctgcggcg tcaggcggct aaaggcttca tcaagaatta
     4261 agagctttgg ctgcatcgcg atggcgcagg cgatgatcac gcgctgtgtc tctccaccgg
     4321 agagtgttgc cgggtgacga tggcgcaacg gctggcactc ggtcagagtc agggcggcat
     4381 caatccgcgc cataatctct gcttcagcaa gacacagatt ttctggccca aaggcaactt
     4441 cttcttccac gctaaaggta cagccggaga gctgcaaata gggcgattgc tggacgagct
     4501 ggatggtggc ggattgttca ttaagcggga gatgcccaat gggcgtaccg aggagcgtcc
     4561 ccgtgcctgt tatctctctt ggcagaaagt ccggatacca gcccgccatc agctgtgcca
     4621 gcgtactttt gccactgccg ttgtcaccaa agatggcgac catccccggt ttgacatagt
     4681 gaaagtcata acagaacgga ggatgcgttg agtgtgtcgg gcaataacgg aactgttcta
     4741 acgtaaccat atccacgctc cgaattctgt cagcatcagc aagatcatgg tgtaacgcgc
     4801 tacgcgttgt agggtgctat cggctggtgc ccacaacgtt gttcgtcggt tgtggatccg
     4861 aaatgcccgc atatccagcg ccgcaccacg gacagccaga tcgttaagcg cattgtgtgt
     4921 tagcggaata attaacgcgg gcatagcacg taaacgctga taccagccct catccagcgg
     4981 cacgccgcgg gcacgctgcg cttcatgaat aatcgctaat tgccgtttta actgctcaac
     5041 gaccaataac ggcccggcaa acagataagc gacgcctggc ggcaggcgag aagcaaacag
     5101 agcgcgaata aaacattgca cggggacgta ctgcattcac aactgtgagg ttgaaacaat
     5161 cgccagaatc agcaaccaga gcgtaatagc atgcgaccac cgttctggcg agcgtggcgt
     5221 gccgcttagc cattctggta gccagccacc gtgtaccagc cacagcccgg ctcccagcga
     5281 gaacatcagc cagacgacat attttgcccg ccgccgcgtg gcctttagcg caataaggca
     5341 gaaaaaggtc gccgcgctat agatcggcaa aatggtctgc gcgggcagga taagcgtggt
     5401 gcaggccgcc aatgcccaca gtgttaatga ggtaaatgga tgcatttagc gcacggcagc
     5461 cattgccgga aaatggcgcg tggtgcgaat cggcagctgg cgcagtaaaa gccagacaat
     5521 caccgctgta agaattttat cgaccaggtt cgcgcctatc accgtaatcg ccaccgactc
     5581 caccaggttt tgccccatcg agtgcatcca ggcaacgaac aaatcggctc cgctaccggt
     5641 gacaccgccg aacagcgcag tacgcagcgg tacagcgacg accgttaccg caagcgtaat
     5701 aatcacgccg ctgacaacga cttttggcag ggtgcgaaac caacccgctc gcgccagcca
     5761 accggagacc aggccgatga ccatcgcgac gggtgcaaat gcggcggcga tcgggtcggt
     5821 cagcaatccc cagagtaaat tggtcagtaa gccggtcagc attccgatca ccggaccaag
     5881 cagtaccgcg ctaataagtg tgccgatgga gtcgagaaat ataggtaatt tgaccatgct
     5941 ggccagttgc ccgccgatca tattgatggc gatagatata acgatcaaca ccagtgcctg
     6001 actggaaaag tggctacgcg ccataagaaa acctctttga aagtagttat ttaacggggg
     6061 aaatatgcaa ctcgtcgtaa ccagaaagcg cgcaaggttg gcggctaaac gccagttcct
     6121 caaggctacc gatcaccagt tcaagcgtgg tccgcacggt acagccgggc atcatatgac
     6181 cgccaagcat cgtgccgtgt ggatcggaaa cgcagagatg aagatgctca ccactctgtt
     6241 ccagcgtgcc gtttagtgag atcacttcga atgtaccgtt caggagcgtg gtgccttctt
     6301 gcccggcata gcgcaaggcg acgtcagtca ggctaccggt acagcctgca atctaggcgg
     6361 catgaagttg atgttgctgt gcaaaagcgt gtagctggga aaaaacttcc tgcccgggga
     6421 ggaggcgcag tgcgtaaaag cgtgcggagg aagaatatga gtagggcaat gtcatcattg
     6481 gagtactccg aaactcggac gtattgcgga taatatcgac gctgcatcag tcatattctg
     6541 atataagtca gggtttgtga cggcacaacg aaaagatttt gctacgctta ttcgtgattc
     6601 tgcaaagtgg aataaattcc acaggggcgg ggctgaaaac gtgacgtaag tcagataatt
     6661 gctttctttg actggacaat cattcctttt attccacgtt tcgcttatcc tagctgaagc
     6721 gtttcagtcg attaaatgtt cgacaattaa ccaatcagtc gcagtttgcg acaggtaagg
     6781 tttccccgga cgatttgctg gattactctg tctggcaaat ttgctcaggg aaaaccttgc
     6841 aggagatcta tgaccgtacg cgtagcgata aatggcttcg gtcgcatcgg gcgtaatgtg
     6901 gttcgtgctt tgtatgaatc cggacgccgg gcggaaatta ccgtggtggc aatcaacgaa
     6961 ctggcggatg ctgcgggcat ggcgcatttg ttgaaatatg acaccagcca tggccgtttt
     7021 gcatgggaag tacgacagga acgcgatcaa ctttttgttg gtgatgacgc catccgcgta
     7081 ttgcatgaac gttcactgca atcgctcccc tggcgtgaac ttggcgttga tgtagtcctc
     7141 gactgcaccg gcgtatatgg ctcccgcgag catggcgaag cgcatattgc cgccggggcc
     7201 aaaaaagtgc tcttttcaca tcctggcagt aacgatctcg acgcgaccgt tgtttacggc
     7261 gtcaatcagg atcaacttcg tgcggaacac cgcatcgttt ctaacgcttc ctgtaccacg
     7321 aattgcataa ttcccgtcat caaattgtta gatgatgcgt acggtattga gtccggcact
     7381 gtgaccacaa ttcactccgc catgcacgat caacaggtta ttgatgcata ccatcctgac
     7441 ctgcgtcgca cccgggcagc cagccagtcg atcattccgg tcgatactaa actggccgct
     7501 ggtatcacac gattttttcc gcaatttaac gatcgctttg aagcgattgc ggtacgtgtg
     7561 ccaaccataa atgtgacggc aatcgattta agcgtgacgg tgaagaaacc tgtaaaagcc
     7621 aatgaagtca acctgttgct gcaaaaagca gcacaaggtg catttcatgg tatagttgac
     7681 tatacggaat tgccgttggt ctctgtagat tttaaccacg atccgcacag tgccattgtc
     7741 gatggcaccc aaacccgggt cagtggcgca cacctgatca aaacgttggt ctggtgcgat
     7801 aacgaatggg gctttgctaa ccgaatgctc gacacgacgt tagctatggc tactgttgct
     7861 ttcaggtaag acgcaagcag cgtctgcaaa acttttagaa tcaacgagag gattcaccat
     7921 gtctgtaatt aagatgaccg atctggatct tgctgggaaa cgtgtattta tccgtgcgga
     7981 tctgaacgta ccagtaaaag acgggaaagt aaccagcgac gcgcgtatcc gtgcttctct
     8041 gccgaccatc gaactggccc tgaaacaagg cgcaaaagtg atggtaactt cccacctggg
     8101 tcgtcctacc gaaggcgagt acaacgaaga attctctctg ctgccggttg ttaactacct
     8161 gaaagacaaa ctgtctaacc cggttcgtct ggttaaagat tacctcgacg gcgttgacgt
     8221 tgctgaaggt gaactggttg ttctggaaaa cgttcgcttc aacaaaggcg agaagaaaga
     8281 cgacgaaacc ctgtccaaaa aatacgctgc actgtgtgac gtgttcgtaa tggacgcatt
     8341 cggtactgct caccgcgcgc aggcttctac tcacggtatc ggtaaattcg ctgacgttgc
     8401 gtgcgcaggc ccgctgctgg cagctgaact ggacgcgctg ggtaaagcac tgaaagaacc
     8461 tgctcgcccg atggtggcta tcgttggtgg ttctaaagta tctaccaaac tgaccgttct
     8521 ggactccctg tctaaaatcg ctgaccagct gattgttggt ggtggtatcg ctaacacctt
     8581 tatcgcggca caaggccacg atgtgggtaa atccctgtac gaagctgacc tggttgacga
     8641 agctaaacgt ctgctgacca cctgcaacat cccggttccg tctgatgttc gcgtagcaac
     8701 cgagttctct gaaactgcac cggctaccct gaaatctgtt aacgatgtga aagctgacga
     8761 gcagatcctg gatatcggtg atgcttccgc tcaggaactg gctgaaatcc tgaagaatgc
     8821 gaaaaccatt ctgtggaacg gtccggttgg cgtgttcgaa ttcccgaact tccgcaaagg
     8881 tactgaaatc gtggctaacg ctatcgcaga cagcgaagcg ttctccatcg ctggcggcgg
     8941 cgacactctg gcagcaatcg acctgttcgg cattgctgac aaaatctcct acatctccac
     9001 tggcggcggc gcattcctcg aattcgtgga aggtaaagta ctgcctgcag tagcgatgct
     9061 cgaagagcgc gctaagaagt aaaaaatcac agggcaggga aacctgcact tgtttcagcg
     9121 cgcttttaga gcacgcattt tttcatactc taaataattc gagttgcagg aaggcgacaa
     9181 gagagtgaat ccccaggagc ttacataagt aagtgactgg ggggagcgaa cgcagacgca
     9241 gcacatgcaa cttgaagtat tatgagtata aggcccgacg atacaggaca agagacatgt
     9301 ctaagatttt tgatttcgta aaacctggcg taatcactgg tgatgacgta cagaaagttt
     9361 tccaggtagc aaaagaaaac aacttcgcac tgccagcagt aaactgcgtc ggtactgact
     9421 ccatcaacgc cgtactggaa actgctgcta aagttaaagc gccggttatc gttcagttct
     9481 ccaacggtgg tgcttccttt atcgctggta aaggcgtgaa atctgacgtt ccgcagggtg
     9541 ctgctatcct gggcgcgatc tctggtgcgc atcacgttca ccagatggct gaacattatg
     9601 gtgttccggt tatcctgcac actgaccact gcgcgaagaa actgctgccg tggatcgacg
     9661 gtctgctgga cgcgggtgaa aaacacttcg cagctaccgg taagccgctg ttctcttctc
     9721 acatgatcga cctgtctgaa gaatctctgc aagagaacat cgaaatctgc tctaaatacc
     9781 tggagcgtat gtccaaaatc ggcatgactc tggaaatcga actgggttgc accggtggtg
     9841 aagaagacgg cgtggacaac agccacatgg acgcttctgc actgtacacc cagccggaag
     9901 acgttgatta cgcatacacc gagctgagca aaatcagccc gcgtttcacc atcgctgcgt
     9961 ccttcggtaa cgtacacggt gtttacaagc cgggtaacgt ggttctgact ccgaccatcc
    10021 tgcgtgattc tcaggaatat gtttctaaga aacacaacct gccgcacaac agcctgaact
    10081 tcgtattcca cggtggttcc ggttctactg ctcaggaaat caaagactcc gtaagctacg
    10141 gcgtagtgaa aatgaacatc gataccgata cccaatgggc aacctgggaa ggcgttctga
    10201 actactacaa agcgaacgaa gcttatctgc agggtcagct gggtaacccg aaaggcgaag
    10261 atcagccgaa caagaaatac tacgatccgc gcgtatggct gcgtgccggt cagacttcga
    10321 tgatcgctcg tctggaaaaa gcattccagg aactgaacgc gatcgacgtt ctgtaagtca
    10381 ttcctttctg cttatctcaa ggcccgctct gcgggtcttt ttttcgccaa aagcaaggtg
    10441 attcagatga gaaatctgtg atctatttgg caaaattatg ctttattgtt tacccttgtc
    10501 agactgcccg tcataaggcg gcggagtgta tttctccatt ttgagtcagt tgaaaaggaa
    10561 tattgaatgg aagatttgaa tgttgtcgat agcataaacg gcgcgggaag ctggctggta
    10621 gctaaccagg cgctgctgct aagttatgca gtaaacatcg tggcggcact cgcgatcatc
    10681 atcgttggtt tgattatcgc gcggatgatt tccaacgcgg tgaatcgcct gatgatctcc
    10741 cgtaaaatcg atgccactgt tgctgatttt ctttctgcat tagtccgtta cggtattatc
    10801 gcctttacgc taatcgctgc cctgggacgc gtgggtgtac aaacagcgtc agtcattgct
    10861 gtactcggtg ccgcaggctt agctgttggt ctggctttgc aggggtcact ttctaacctg
    10921 gccgctggcg tgttacttgt catgttccgc ccgttccgtg ccggagaata tgttgacctc
    10981 ggcggcgtag ccggtactgt gctgagtgtg cagattttct ccaccaccat gcgtactgca
    11041 gacggtaaaa ttatcgttat tccgaacggt aaaattattg ccggaaatat tattaacttc
    11101 tcccgcgagc cagttcgccg taacgaattt attattggcg tggcgtatga ttccgatatc
    11161 gatcaggtta agcagatcct gaccaatatt atccagtctg aagatcgcat tttgaaagat
    11221 cgcgaaatga ctgtgcgcct gaacgaactt ggtgcatcgt cgattaattt cgtggtccgc
    11281 gtctggagca acagcggcga tctgcaaaac gtgtactggg atgtgctgga gcgtattaaa
    11341 cgtgaatttg atgccgccgg tatcagcttc ccgtacccgc aaatggatgt gaactttaag
    11401 cgggtgaaag aagacaaagc tgcgtaatca acgctgaggc cagataatac tctctggcct
    11461 ctctcttatt agttattctg attgccaatt aatattatca atttccgcta ataacaatcc
    11521 cgcgatatag tctctgcatc agatacttaa ttcggaatat ccaacgtgtt ttcttattac
    11581 tttcaaggtc ttgctcttgg ggcagctatg attctgccgc tcggcccaca aaatgctttt
    11641 gtgatgaatc agggcattcg tcgtcagtac cacattatga ttgccttact ttgcgctatc
    11701 agcgatttgg tcctgatttg cgcggggatt tttggtggta gcgcgttatt gatgcagtcg
    11761 ccgtggttgc tagcgctggt cacctggggc ggcgtagtct tcttgctctg gtatggtttt
    11821 ggcgctttta aaacagcgat gagtagtaat attgagttag ctagtgccga agtcctgaag
    11881 caaggcagat ggaaaattat cgccaccatg ttggcagtga cctggctgaa tccgcatgtt
    11941 tacctggata cttttgttgt actgggcagc cttggcgggc agcttgacgt cgaaccaaaa
    12001 cgttggtttg cgctcgggac aattagcgcc tctttcctgt ggttctttgg tctggctatt
    12061 ctcgcagcct ggctggcacc gcgcctgcgc acggcaaaat cacagcgcat tatcaatctg
    12121 gtagtgggat gtgtcatgtg gtttatagcc ttgcagctgg cgagagacgg tattgctcat
    12181 gcacaagcct tgttcagtta ggcgctatct gatggaaaaa taaaacagag gcgctaagct
    12241 tgcctccaga ggtcctgaat tttgggcaag tcaattaaca cggagagact aacgtgaagt
    12301 tcaaagttat cgccctggcg gcattaatgg gtattagcgg gatggcagcg caggctaacg
    12361 aattgccgga tggaccgcat attgtcacct ccggtacggc aagtgtggat gcggtgccag
    12421 acattgccac tcttgcgatt gaagttaacg tggccgcgaa ggatgccgct acagccaaga
    12481 aacaggcaga tgagcgcgtc gcacaataca tttccttcct tgaactcaat cagatcgcga
    12541 aaaaagatat cagctcagcg aatttacgca cccagccaga ttatgactat caggatggta
    12601 aaagtatcct gaaaggctac cgagctgtga gaacggtgga agtcacgctc cgtcagttag
    12661 acaaactgaa ttccttgctg gatggcgcgc tgaaggcggg tcttaacgaa attcgttctg
    12721 tgtcgctggg cgtggcgcag ccggatgcct ataaagacaa agcgcgtaag gcagcgattg
    12781 ataacgcgat tcatcaggcg caggaactgg cgaacggctt tcatcgtaaa ctggggccgg
    12841 tatatagcgt gcgctaccat gtttccaact atcagcccag cccaatggtg cggatgatga
    12901 aagccgatgc cgcgccggtg tctgcccagg aaacttacga gcaggccgct attcagtttg
    12961 atgatcaggt cgatgtggtc ttccagttag aacctgtgga tcaacaaccc gctaaaacac
    13021 ctgcagcaca ataatacttg tagcccgacg tattcgtcgg gctaatgcct ttacccgata
    13081 ttgcctcctt tttctgtaat tttctgtaaa accatgacgt aatcacctat ctttgctcgc
    13141 ggtttcatca aggataggct gactgaaacg gttaagcgga caaggatgcg gggagggggg
    13201 atggacattt ttatttcgaa aaagatgcgc aatttcattt tattagcgca aaccaataat
    13261 attgcccggg cggcagaaaa aatccatatg acggcttcgc catttggtaa aagcattgcc
    13321 gcgctggaag aacaaattgg ctatacgcta tttacccgca aagataataa tatcagcctc
    13381 aataaagccg gtcaggaact gtatcaaaaa ctgttcccgg tttatcaacg actttctgcg
    13441 attgataatg aaatccataa ctcagggcgt cgttcacgag aaattgtgat tggcatagat
    13501 aatacttatc cgacaattat tttcgatcag ttgattagtc tcggagataa gtatgaaggc
    13561 gtgactgccc agccagttga gttcagcgaa aacggcgtta tcgataatct ttttgaccgt
    13621 cagttagatt ttattatctc cccacagcat gtatctgcac gagttcagga actggaaaac
    13681 ctgactatca gtgaattacc accgctgcga ttgggctttc tcgtttctcg ccgttatgaa
    13741 gagcgtcaag agcaagagct gcttcaggag ctgccctggc tacaaatgcg ctttcaaaac
    13801 cgggctaact tcgaagcaat gattgatgcg aatatgcgac cttgtgggat caatcccacc
    13861 attatttatc gtccctacag ttttatggcc aaaatcagcg ccgtggagcg cggacatttt
    13921 ctgacggtga tcccgcattt tgcctggcga ctggtgaatc cggcaacgct gaaatatttt
    13981 gatgcgccgc acaggccgat gtatatgcag gaatatcttt attcaatcag aaatcatcgt
    14041 tataccgcca cgatcttcag catattgctg aagatcgtga cgggacaaac cattaaccca
    14101 gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt
    14161 gaatatgtcc gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat
    14221 gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg
    14281 cgatcccttg ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc
    14341 acattggcac gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg
    14401 acagatacgc gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc
    14461 cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag
    14521 cgatgacgcc cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca
    14581 caatgcggct ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga
    14641 ttgtcaggct ggaggcgctg gccccgctga ttttgccggt ttccagtaaa tgcaccaccg
    14701 attcctgtag cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac
    14761 gtgccattac cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg
    14821 gaatacgccc atgcgccatt tcctgcaata agaacgtgac cacgttatcg gcaatctgct
    14881 ggcacatggg attttgctta tccagcatat taccggcgtc gggcaagttg gtttccacga
    14941 cggcgacaat ctttttcgga tcgatttgca catagcgggt accgacgcga tccattgcat
    15001 ggaagatcga cacgctattg cgccgcggtg gcgcgccagg aatcacaata tctgccagtt
    15061 ctgcaacgcg cggatcgtga tagtgattaa gttcaatgat cactttcttc gctcgcagca
    15121 gccaggtcgg cgcattaccg atcccgctgg ttaaccagac tcgaccatcc ggtgccagtg
    15181 ccgatgcttc aatgacggca acatcaatgt cgccgaagaa accgtaattg accatttgcg
    15241 ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc ctgattgatc tttttacgta
    15301 aactggacga tgtttgatat ggcgcacgcc aggaaacagc atcggcgtca gaaagtacat
    15361 cgtcagcggc ggcgctgatt gacgcccccg tcagcaggcg aatttgatac ggctttttgg
    15421 cctcatgctg ttcgttagct ctgcgggcaa tcgcggcggg tagggctttc ggcgaacccg
    15481 ctggggtaaa gccgctaaat gccaccatgt cgttatgctg gataatttct gccgcttcat
    15541 cggcggtcat ccttgtccac tgagtttcca tttttactcc ttcgttcgca gggattaatg
    15601 accaacgaaa ttaggtttac gtttttcgag gaaagcgttc atcccttcct ggtaatcttc
    15661 gctgtcataa accgcgcggc gcatcccctg aatacgttca aattcatcgg agttcatggt
    15721 gtgtgcttcg cccagtacac gcagctcttc tttgataacg gcaatggcta acggcgcttt
    15781 ctcagagatg tggtgcgcca tttgtaaggt gaaatcttcc acttccacaa catggttgag
    15841 gatgccgaca gccagcgcgc gctgggcggt gattggcgaa gcggtaaaaa tcagctcttt
    15901 gacaatgtgg aagcccgcgt cgcgggtcag gttgtgaatg ccgaccaggt tatacgggac
    15961 gccgaggttt acaggcgtca ttgagaaggt tgaggtactg gcggcgatga tcagatcgga
    16021 actcatgatc atttcaaatg cgccacccca aacactacct tccaccatcg aaatgatcgg
    16081 tttcgggaat ttttggatca tgcgggtgat ttgacgcaat ggatcatcat aggagagcgg
    16141 atcgcgaccg ccagacggca gttcgtgaat atcgtgacct gcggagaaga ctttggatcc
    16201 acttggcgcc cgcaaaatga tacagcgaat ttccggccgg ttgagatcgc ttaacgcctg
    16261 cataagatca tcaataaaga ctttacttaa ggcattaagt tttcgtccat agttaaactc
    16321 aatgaccgcc actttgttga tagtggcaac gttcacatac tgataagaca taaaaattcc
    16381 tttaatcaaa atattgcgtc tggataaatt cactgagctg ccgcaggccg gtgcgcggtg
    16441 agagcgtatt gtttttgacc gctaaaagcg tctggtggta atagcgatcg aaatcttcat
    16501 tcgcgaacag gtgattcagt acttcttctt cggtctgctt acgcagccat tccaccgatt
    16561 gttgttgccg cacttgttgt aaacgaccac tggcagttag cgcggttttg aagtcgatga
    16621 tggcgtgcca gatctcatcg attccacgtt tttccagtgc gctacaagtc agaccccgtg
    16681 gctgccattc gtcgtattta cgtcgcagaa tatgcagggc actctcgtac atatgccggg
    16741 caatggcgac attggtatgg ttatcgccat cgtctttgtt gataacgagc agatcagcca
    16801 cttccatcag ccctttttta atgccctgca gatcatcgcc gccaccggca atttgcaacg
    16861 agataaaaca gtccaccatg cgggcgactt ccgtttccga ctgcgcgacg ccaaccgttt
    16921 cgacaatcac tacgtcataa cccgccgctt cgcataacag cattaattcc cgtgctcgct
    16981 gactggcacc gcccagatga ccggaggagg gtaccgggcg aataaacgcc gcctcggcac
    17041 gcgccaggtc attcatgcgg gttttatccc cgagaatgct accgccagtg accgggctgc
    17101 tgggatcgac cgcaataaca gcaaccttta atccctcacg aatcaacaac atgccaaagg
    17161 cttcaagaaa ggtacttttc cccgcgccgg gtgtgccggt aacgcccagt cgcagggcgt
    17221 taccacagaa cggcataata gcgtcaagca gctgtgtact tagcgcctga tgacgtgggt
    17281 gacggctttc caccagcgtc atggcttggg cgagcgtggc acgctcaccc tgacgtaagc
    17341 ggcgaatact ttctgccagc gtggcttcat taatcatgat gctggcttat cagattcagt
    17401 acgtcgcgca cactgtcgag cataggtgta cctggaccat aaatcgccgc cacgccgcgc
    17461 tcttgcagga aggcgtaatc ctgcggcgga atgacgccac ccgcgaccac gcagatatct
    17521 tcgcgtcccc attttttcag cgcttcgacc agttccggga tcagcgtttt atgaccggca
    17581 gccagtgagg atgcgcccac tacgtgaacg tcgttttcta cggccaggcg ggcgatctct
    17641 tcaggtgtag agaacatcgg gcttaaatct acgtcgaaac cgagatcgga ataggcgctg
    17701 gcgatcactt tcgcgccgcg atcgtgtcca tcctggccca tcttagcgat cagaatgcgc
    17761 gggcgacgac cattgtcggc aaggaactgc tccgtttgcg caacaatggc atcgaactcg
    17821 gaggccgatt tctcagactg atgatagctt tgcgcaatca cgccggtaac acactggctt
    17881 ggcaccagat aacggtcgaa agcgacttcc agcgcatcgg aaatttcacc cagggtggcg
    17941 cgaacgcgag cggcattaac agcggcagcc agcaggtttt cgttatgctg tgcggcgtga
    18001 gtcagggcgt tcaacgcggc ggttacggcg gcatcatcac gggtggcgcg aatgcgttcc
    18061 agcgaagcaa tttgctcgtt acgcaccatc acgttgtcga tctcaagtac atcggtttcg
    18121 tcttcgtgat ccagtttgta cttgttgaca ccaacgatga cacgcttgcc ctggtcgatc
    18181 agcgactgtt cgcgcgctga ggcctcttcg atcattcgtt ttggcagacc tgcttcgatc
    18241 gctttcgcca tgccaccggc ttcgtcgatc tgttggataa tagctctggc ttgtttgacg
    18301 atttgatcgg tcagcgactc aatgtaatag gatccggcca gtggatcgac ggtgtggcag
    18361 agttctgatt cttcctggat gatgatctgg gtgttgcggg caatgcgtgc tgagaaatcg
    18421 gtaggcaaac caagcgcttc gtcaaaggcg ttggtatgca gtgactgagt accgcccagc
    18481 gtcgcagcca gtgcttcaat ggtggtgcgg ataacgttgt tatacggatc ctgttcagtc
    18541 aggctccagc ctgaggtctg gcagtgggta cgcagcgcca gtgatttcgg atcctgtgcg
    18601 ccaaatccac tgaccgcttc gctccataaa taacgtgccg cacgcaacat ggcgacgttc
    18661 ataaacagat ccatgccgat accgaagaat aacgacaggc gaggagcgaa gtcatcaatt
    18721 ttcagtccgg cagagattgc tgctttgatg tactcaatcc catcggcgat cgtaaatgct
    18781 acctgctgca cgcagttggc acccgcttcc cccatgtggt aaccgctgat actgatggta
    18841 ttaaatcgcg gcatgttgcc ggaacaccag gcgatgatgt cggcgataat gcgcattgac
    18901 ggttttggtg ggtaaatata ggtgttgcgg cagaggtact ctttgagaat atcgttttga
    18961 atggtgccgg tcagtttatc aggtgtaaca ccttgctctt ctgcggcgac gatataaaac
    19021 gccagtactg gtagcactgc gccattcatg gtcatcgaaa ccgacatttt atccagcggg
    19081 atctggtcga acaggacttt catatcttcc acggtgtcga tagcgacgcc cgctttgccg
    19141 acgtcacccg ccacgcgtgg gttatcggag tcgtagccac ggtgggtggc aaggtcaaac
    19201 gcaacggaaa gacctttttg cccggcggcc aggttacggc gataaaaagc gttggactct
    19261 tttgctgttg aaaaaccagc atactgacgg atggtccacg gttgggcggt atacatagtg
    19321 gcacgcgggc cacgaacgta gggcggcaaa ccaggaaggg tacctgtcac ctccagatta
    19381 tcgagatcgg cttcggtata cagcggcttg atggcgatcc cttccgcggt ttgctgaacc
    19441 agcgagtcga cagttttctc ccgacggctc aattccttgt tggcaagctg ttgccactcc
    19501 tgctcgttag acattatttg ctccgtaaaa cgcaatcaaa atgagggtat ttgcgcttac
    19561 ggtgagcttt ttacgtgggt ttgtcgccag caaaaaaaat gcattcagac gtcgtttttt
    19621 gccgcctgag atttgtctat tttgtgatgt gattcaagga atacaaaaag aagtgaggac
    19681 aacgcctgat atgtgctatc aggcattatt tgatggatta atcctgacga aggactttgt
    19741 gaccataatc gagtaacgca tcagtgactt tacgcatcat gcggctttca ggagcaaagc
    19801 ggtgccagta gagcatccgt cgttgaaata gcccaggcgt taagtcaatc agttcaccgc
    19861 tggccagctc tttctcgatt tgcaggtgcg ggatcataca gcaggtggtg ccctggcgag
    19921 caagttgtac gaacgcttct gaagaattaa cgatatggca gggtacgctg ccaggaggaa
    19981 gatcgaagtt ttgctgcaaa aaggcctggt gcatatcgtc aagatggtca aacgcgacca
    20041 ctggcgcttt caataatgcc gaacgcgtta cgccgttagg aaaatatttt tcggcaaacg
    20101 gttttgagct gacgaacaga tagtcgagcg caccgagttt atcgacaaga caactcggca
    20161 gcgcctgatg ttgaatactc accgcgccga ccacttcgcc gcggcgcaga cgttcctgag
    20221 tacgggtttc atcttctacc tgcaagttga ggcggatagg cgaatcagcc aacacaggag
    20281 ccagtgccgg aagcaaccac gtcgccagac tgtcggcgtt gaccgccagt gaaagcagca
    20341 gcggagtcga accggtttgt tcatcgccca gccactcttc ttccagcaac tccacctggc
    20401 gcagcagtgc cagcagtttt tgcccttgtt ccgtcgggcg cggcggtacg gtacgcacca
    20461 acagcggctg cccgaacata ttttccagtt gcttaatgcg ctgtgagacg gctgattgtg
    20521 taatgcacag cttttgtgcc gcgcgctcaa atcctcgttc acgtatcacc gcatccagtg
    20581 cctgtaatgt tctgtagtcc gggcgtttca ttgctgttgt ctgctcctgg taaatgcttt
    20641 ttcctgcact atgacacaat tttgtgtcag gttgcatata ccgaagccgt cggcgggtaa
    20701 catttgtatc aatggttaaa cgcgcagctt aaaagatcag ggatttgcgc tattgcattt
    20761 cgcttagcga cttcgcgccc tggttatgtt gaatggcgtg gcgttattgc ctctgttcgc
    20821 accgtggagc ggcataccca gaccgttgta tgctttattt gcggggcgaa aggggatgcc
    20881 cgccattgcg cgatatttta tggatgagtt aaccacccag cttgccaacg gggtctgaat
    20941 cgcttttttt gtatataatg cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc
    21001 aggatgaatt gaaaaaagca gtaggatggg cggcacttca gtatgttcag cccggtacca
    21061 ttgttggtgt aggtacaggt tccaccgccg cacactttat tgacgcgctc ggcacgatga
    21121 aaggccagat tgaaggggcc gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc
    21181 tcggcattca cgtttttgat ctcaacgaag tcgacagcct tggcatctac gttgatggcg
    21241 cagatgaaat caacggccac atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg
    21301 aaaaaatcat tgcttcggtt gcggaaaaat ttatctgtat tgccgacgcc tccaagcagg
    21361 ttgatattct gggtaaattc ccgctgccag tagaagttat cccgatggca cgtagtgcag
    21421 tggcgcgcca gctggtgaaa ctgggcggtc gtccggaata ccgtcagggc gtggtgaccg
    21481 ataatggcaa cgtgatcctc gacgtccacg gcatggaaat ccttgacccg atagcgatgg
    21541 aaaacgccat aaatgcgatt cctggcgtgg tgactgttgg cttgtttgct aaccgtggcg
    21601 cggacgttgc gctgattggc acacctgacg gtgtcaaaac cattgtgaaa tgatctgacg
    21661 ggggaacctc ccccgttaaa aaaattctct tcattaaatt tggtgacatg tgtcacgctt
    21721 ttaccaggca attgtcgatt gctctaaata aatcctctaa accagcatat tcatccaaga
    21781 attacctttg cgtgatattt cctcaacatc gcgacgcaaa cgttcatatt gccgcaatat
    21841 tattttttga tatgttgaaa ggcggatgca aatccgcaca caacatttca aaagacagga
    21901 ttgggtaaat ggcaaaggta tcgctggaga aagacaagat taagtttctg ctggtagaag
    21961 gcgtgcacca aaaggcgctg gaaagccttc gtgcagctgg ttacaccaac atcgaatttc
    22021 acaaaggcgc gctggatgat gaacaattaa aagaatccat ccgcgatgcc cacttcatcg
    22081 gcctgcgatc ccgtacccat ctgactgaag acgtgatcaa cgccgcagaa aaactggtcg
    22141 ctattggctg tttctgtatc ggaacaaacc aggttgatct ggatgcggcg gcaaagcgcg
    22201 ggatcccggt atttaacgca ccgttctcaa atacgcgctc tgttgcggag ctggtgattg
    22261 gcgaactgct gctgctattg cgcggcgtgc cggaagccaa tgctaaagcg caccgtggcg
    22321 tgtggaacaa actggcggcg ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg
    22381 gctacggtca tattggtacg caattgggca ttctggctga atcgctggga atgtatgttt
    22441 acttttatga tattgaaaac aaactgccgc tgggcaacgc cactcaggta cagcatcttt
    22501 ctgacctgct gaatatgagc gatgtggtga gtctgcatgt accagagaat ccgtccacca
    22561 aaaatatgat gggcgcgaaa gagatttcgc taatgaagcc cggctcgctg ctgattaatg
    22621 cctcgcgcgg tactgtggtg gatattccgg cgctgtgtga tgcgctggcg agcaaacatc
    22681 tggcgggggc ggcaatcgac gtattcccga cggaaccggc gaccaatagc gatccattta
    22741 cctctccact gtgtgaattc gacaacgtcc ttctgacgcc acacattggc ggttcgactc
    22801 aggaagcgca ggagaatatc ggcttggaag ttgcgggtaa attgatcaag tattctgaca
    22861 atggctcaac gctctctgcg gtgaacttcc cggaagtctc gctgccactg catggtggtc
    22921 gtcgtctgat gcacatccac gaaaaccgtc cgggcgtgct aactgcgctg aacaaaatct
    22981 tcgccgagca gggcgtcaac atcgccgcgc aatatctaca aacttccgcc cagatgggtt
    23041 atgtagttat tgatattgaa gctgacgaag acgttgccga aaaagcgctg caggcaatga
    23101 aagctattcc gggtaccatt cgcgcccgtc tgctgtacta attccccttc tctgaaaatt
    23161 aacgggcagg tcactgactt gcccgttttt ttatcccttc tccacaccgt tgccgcgttg
    23221 cgcattctac tttgcgagtc tgctccagaa aataaatcaa agtcagatca atgcgttgtg
    23281 tttatttaat ggggatttac gatggcggga cagcatgggg ctgtaacggt aaagccctca
    23341 ccgaagcgag ggcttaaagg agaagggtta tgatgcgact tgtcatcata ctgattgtac
    23401 tgttactgat aagtttcgga gcttattaac agtcagtctc aagggaggag tcatcctccc
    23461 ttacccttac tcactaaatt aggtcaaaga atcaacgatg tcaatcaggg cgatgcgggg
    23521 tgcatcgccc tgaaccgaac tttttgatag ccacttcatc gcccacgttt actgccgtaa
    23581 tagatccttt ttcccaaaaa ttatctctgt tttgcatggg gtttttcgag cacgctcgaa
    23641 gaaaagattt aaaggtagat caatgcgttg tggttattta aagagagctt acgatagctg
    23701 ggcagcatgg ggctgtaacg gtaaagccct caccgaagcg agggcttgaa ggagaagggt
    23761 tatgatgcga cttgccatca tactgattgt actgttactc ataagtttcc cagcttatta
    23821 acagtcagtc acaggggagg agcaatcctc ccttaccctt actcactaaa ttaggttaaa
    23881 gaatcaacga tgtcaatcag ggcgatgcag gtgtatcgcc cttaccactc ccagactttc
    23941 gacggtgtaa ccaccgcagg aagagggata tcccactctt caacggggag tttttccacc
    24001 aactgacaat catgcgcata acccaccggt tgcgttttat agtgctgcca gttttgtaag
    24061 gtccgatcat aaaaaccacc gcccattccc aggcgctgac cgtactcatc aaaggcgacc
    24121 agcggtgtga tcagcacgtc taatcgggaa aggggtagca cgtcacgcac atccagtttt
    24181 ggctcatgga tcttcaacct gttcatcacc agttcgcttt gcggatggta attcaggaac
    24241 agcaaattac cggcactaaa gggatgcaaa actggaagat atacgcgctt accggcgcgc
    24301 cagagttgtt ctattagcgg ctgggtgtcg agttcaccat caaaagagag gaatacagcg
    24361 accgtatgcg ccatcaccac cgggggataa gtcatcatcc gggtagcggc ttgttgaccc
    24421 atttccagct gttgttccgg cgttaacgca cgacgacgtt gccgaatcat tttgcggatt
    24481 tcttgtcggg ataatgtcag tgggagctct ggtagttgcg tcatggctgg tgccagataa
    24541 gaagggaatc tccgagatgc cgccgcaggc tgtaaccctt gaacccttgg ttcaaggtga
    24601 atgtgtcgtc gcagttttaa ggcttctcgg acgaaccgag catgctcacc aaccgcggag
    24661 cgccacattc ttgtggtatg aaatatcggc tcaggggact ggcccgcttg cgaacatctc
    24721 agagaaattt tgtcttcacg gttactctac cacagtaaac cgaaaagtgt cattcaaagt
    24781 tttggttagt tttttcggtg atgcgacctt gttcaagtaa cgcttgttct atggtctgct
    24841 gcagcatccg aatacgctgt tccatacttg ccgcgtagtc acgagtcttt gctttttctt
    24901 gcgctaactc atagctgata ttcaatgcgg caatgaagac caactgttca gtatttgtga
    24961 ctctagtgcg ttctttcaga tcttgcaacc gttggttcag atcgtccgct gcctgattca
    25021 acgcatccct ttggtcaggc gggcagttca cacgcagtga acggccaaaa atttggatat
    25081 cgacgggttg tgcagacatg ccaccttcct gctgattgac tgcgctgcct tcgtctttag
    25141 acccatggtc tgcgaagggg cgacactata gctaccctga tgagaagaga caagcccttt
    25201 tctggtccac cagggcccaa agtggtagca tatcatgaat attcctccct ttgacgacga
    25261 atgcttatgt ctatacagaa cgaaatgcct ggttacaacg aaatgaacca gtatctgaac
    25321 caacaaggga cgggtctgac ccctgccgag atgcatggtt taatcagtgg gatgatatgt
    25381 ggcggtaacg atgacagctc atggctgccg ctacttcacg acctgacgaa cgaaggcatg
    25441 gctttcggtc atgagctggc acaggcactg cgtaaaatgc actctgccac cagcgatgcc
    25501 ctgcaggatg acggcttcct ttttcagctt tatctgcctg atggcgatga tgtcagcgtt
    25561 ttcgatcggg ctgatgcact ggcaggttgg gtcaatcact tcctgcttgg tcttggcgtt
    25621 acgcaaccga agctggacaa agtgaccggc gaaaccggtg aagccatcga cgatctgcgt
    25681 aacatcgcgc agctgggcta cgacgaagac gaagatcagg aagagcttga aatgtcgctt
    25741 gaagagatca tcgaatacgt tcgtgttgcc gcgctgttat gccacgacac ctttactcat
    25801 ccgcaaccga ccgcgccaga agtacaaaaa ccgactctac actaaaaaca aaaaacgtaa
    25861 ggagagtgtt atgagtgaga tatcccggca agagtttcag cgtcgccgtc aggccctggt
    25921 ggagcaaatg caacccggca gcgccgcgct gatttttgct gcaccagaag taacacgtag
    25981 cgccgacagc gaatacccct atcgtcagaa cagtgacttc tggtacttca ccggctttaa
    26041 cgaaccggaa gcggtgctgg tgctgattaa aagcgatgac actcataacc acagcgttct
    26101 gtttaaccgc gttcgcgacc tgacggcgga gatctggttt ggccgtcgct taggccagga
    26161 tgccgcgcca gagaaactgg gcgttgaccg cgcactggca ttcagcgaaa tcaatcagca
    26221 actttatcaa ctacttaacg gcctggatgt ggtttaccat gcccagggcg aatatgcata
    26281 tgctgatgaa atcgtgaaca gtgcgctgga aaaactgcgt aaaggttcgc ggcaaaatct
    26341 caccgcaccg gcaacgatga tcgactggcg tcctgttgtt catgaaatgc gcctgttcaa
    26401 atcgccagaa gagattgccg tactccgccg cgcgggagaa atcaccgcca tggcacatac
    26461 acgggcgatg gaaaaatgcc gtccgggaat gttcgagtac catctggaag gcgaaattca
    26521 ccacgaattt aaccgccacg gtgcgcgcta tccttcctat aacaccattg taggcagcgg
    26581 tgaaaacggc tgcattctgc actacaccga aaacgagagt gaactgcgcg acggtgacct
    26641 ggtgttgatt gacgcgggtt gtgaatacaa aggttacgcg ggcgatatta cccgcacctt
    26701 cccggtcaac ggcaaattca cccaggccca gcgtgaaatc tacgacattg tgctggagtc
    26761 tctcgaaacc agcctgcgcc tgtatcgtcc gggaacttcc attctggaag tcactggtga
    26821 agtggtgcgc atcatggtta gcggcctggt aaaactcggc atcctgaaag gtaatgttga
    26881 tgaactgatc gctcagaacg cccatcgtcc tttctttatg catggcctta gccactggtt
    26941 aggactggat gtccatgacg ttggcgttta tggtcaggat cgctcgcgca ttctggaacc
    27001 ggggatggta ctgaccgtag agcctggtct gtatatcgcg ccggatgcgg atgtgccaga
    27061 acaatatcgc ggtatcggca ttcgcattga agacgacatt gtgattaccg aaaccggcaa
    27121 cgaaaacctc accgccagcg tggtgaaaaa gccggaagaa atcgaagcgt tgatggctgc
    27181 tgcgagaaag caatgagcgt aatcatcgtc ggtggcggca tggcgggcgc gacgctggcg
    27241 ctggctattt cccggttaag tcacggggcg ctgccggtac atttgattga agcgactgcg
    27301 ccagagtcac atgctcatcc gggctttgat ggacgagcga tagcgctggc ggcgggtacc
    27361 tgtcagcaac tggcgcgcat cggcgtctgg caatctctgg ctgattgcgc aaccgccatc
    27421 actaccgtgc atgtcagcga tcgtggtcat gctggattcg tcaccctcgc cgcagaagat
    27481 taccaactgg cggcgctggg gcaggttgtc gaattgcata atgtcgggca acggctgttt
    27541 gcattgctgc gaaaagcacc tggcgtaatg ctgcattgcc ccgatcgcgt agctaacgtt
    27601 gcccgtactc agagtcatgt tgaagtgacg ctggagagcg gcgagatgct gacgggccgc
    27661 gtgctgatag ccgctgatgg cacccattca gcgttagcca ccgcgtgcgg cgttgattgg
    27721 cagcaggagc cttacgaaca actggcagtg attgccaacg ttgctacttc tgttgcgcac
    27781 gaaggccgcg cttttgaacg cttcacgcaa catggcccgc tggcgatgtt gccgatgtct
    27841 gacggacgct gttcgctggt ctggtgtcat ccactggaac ggcgcgaaga ggtgctgtcg
    27901 tggagcgacg agaagttttg ccgtgaactc cagtctgcct ttggctggcg actggggcaa
    27961 attacccacg ccggtaaacg cagtgcttat ccgctggcat taacccgcgc cgccagaccg
    28021 attacccatc gcactgtgct ggtgggcaat gcggcgcaaa ctctgcaccc gattgccggg
    28081 caagggttta acctcggtat gcgagatgtc atgagccttg cggaaaccct gactcaggcg
    28141 catgagcgcg gagaagacat cggtgattac ggcatattgt gccgctatca gcagcgtcga
    28201 cagagcgatc gcgaagcaac catcggcgtc acggacagcc tggtacatct ttttgccaac
    28261 cgttgggcac cgctggttgt cgggcgcaac atcggactga tgacgatgga attattcacc
    28321 ccggcacgcg atgtgctggc gcagcgcacc ctcggttggg tggcgcgttg aaggcggttt
    28381 taaggaataa acaatgcaaa gtgttgatgt agccattgtt ggcggcggca tggtggggct
    28441 ggcggttgcc tgcggcttac aggggagcgg cttacgcgtt gccgtactgg agcagcgcgt
    28501 accggaacct ctggcggtag atgcactacc acaactgcgc gtttcggcta tcaatgccgc
    28561 cagcgaaaaa ttactcaccc gtcttggcgt ctggcaggac attctcgctc gtagggccag
    28621 ttgttatcac ggcatggaag tgtgggacaa agacagtttt ggtcacatct cgtttgacga
    28681 tcaaagcatg ggctatagcc atcttgggca catcgttgaa aactcagtga ttcactacgc
    28741 gctgtggaac aaagcgcagc agtcgtcaga tattactctg ttggccccca cagaattaca
    28801 gcaggtcgcc tggggagaaa atgaaacctt cctgacgctg aaagatggca gcatgttaac
    28861 ggcgcgtctg gtgattggcg cggacggtgc taattcctgg ttgcgcaaca aagcagatat
    28921 tccgctgact ttctgggatt atcagcatca cgcgctggta gcgaccattc gcacggaaga
    28981 accgcatgat gcggtggcgc ggcaggtttt ccatggcgaa ggcattctgg cctttttacc
    29041 gcttagcgat ccgcatcttt gctcgattgt ctggtcactg tcgccagagg aagcgcagcg
    29101 gatgcagcag gcaagtgaag acgaatttaa tcgcgcgtta aatatcgctt ttgataatcg
    29161 ccttggctta tgcaaggttg agagcgcgcg tcaggtgttc ccgctgacgg ggcgttatgc
    29221 gcgccagttt gccgcacacc gtctggcgct ggtgggcgac gccgcgcata ccattcaccc
    29281 actggcgggg cagggcgtta atctcggatt tatggacgcc gcagagctga ttgctgaact
    29341 gaaacggttg catcgtcagg gtaaagacat cgggcagtac atttacctgc gccgctatga
    29401 acgtagccgc aagcacagcg cggcgctgat gctggctggt atgcagggtt ttcgcgatct
    29461 cttttccggc gctaatccgg cgaaaaaact gctgcgtgat attggtttga aactggccga
    29521 cacgcttcct ggcgttaagc cgcaacttat ccgccaggca atgggattaa acgatttgcc
    29581 tgaatggctg cgttaaaaat ttctcctctg ttgtttattt gatacccatc acactttcat
    29641 ctcccggttt tttcgccggg agattttcct catttgaaat aaactaattt cacctccgtt
    29701 ttcgcattat attttctaat accatgattt tttgatttag tgttttttga cattttttta
    29761 gctcttaata ttgtcttatt caaattgact ttctcatcac atcatctttg tatagaaatt
    29821 ggtgtatttt ttggtttttt attctgtcgc gatttttgca ttttttaacc ataagctaat
    29881 gtgatgatca attttacctt atggttaaca gtctgtttcg gtggtaagtt caggcaaaag
    29941 agaacgattg cgttggggac cgggagtggc tccgatgctg ggttttgtgg tgataatttc
    30001 accatgaaaa agttgtcagc cccgcttatt caatgaggac aagatggcac aacagactcc
    30061 tttgtacgaa caacacacgc tttgcggcgc tcgcatggtg gatttccacg gctggatgat
    30121 gccgctgcat tacggttcgc aaatcgacga acatcatgcg gtacgtaccg atgccggaat
    30181 gtttgatgtg tcgcatatga ccatcgtcga tctccgcggc agccgcaccc gggagtttct
    30241 gcgttatctg ctggcgaacg atgtggcgaa gctcaccaaa agaggcaaag ccctttactc
    30301 ggggatgttg aatgcctctg gcggtgtgat agatgatctc atcgtctact actttactga
    30361 agatttcttc cgcctcgttg ttaactccgc cacccgcgaa aaagacctct cctggattac
    30421 ccaacacgct gaacctttcg gcatcgaaat taccgttcgt gatgaccttt ccatgattgc
    30481 cgtgcaaggg ccgaatgctc aggcaaaagc tgccacactg tttaatgatg ctcagcgtca
    30541 ggcggtggaa gggatgaaac cgttctttgg cgtgcaggtg ggcgatctgt ttattgccac
    30601 cactggttat accggtgaag cgggctatga aattgcgctg cccaatgaaa aagcggccga
    30661 tttctggcgt gcgctggtgg aagcgggcgt taagccctgc ggtctggggg cgcgtgacac
    30721 gctgcgtctg gaagcgggca tgaatcttta tggtcaggag atggacgaaa ctatttctcc
    30781 tttagccgcc aacatgggct ggactatcgc ctgggaaccg acagatcgtg actttatcgg
    30841 tcgtgaagcc ctggaagtgc agcgtgagca tggtacagaa aaactggttg gtctggtgat
    30901 gaccgaaaaa ggcgtgctgc gtaatgaact gccggtacgc tttaccgatg cgcagggcaa
    30961 ccagcatgaa ggcattatca ccagcggtac tttctccccg acgctgggtt acagcattgc
    31021 gctggcgcgc gtgccggaag gtattggcga aacggcgatt gtgcaaattc gcaaccgtga
    31081 aatgccggtt aaagtgacaa aacctgtttt tgtgcgtaac ggcaaagccg tcgcgtgatt
    31141 tacttttttg gagattgatt gatgagcaac gtaccagcag aactgaaata cagcaaagaa
    31201 cacgaatggc tgcgtaaaga agccgacggc acttacaccg ttggtattac cgaacatgct
    31261 caggagctgt taggcgatat ggtgtttgtt gacctgccgg aagtgggcgc aacggttagc
    31321 gcgggcgatg actgcgcggt tgccgaatcg gtaaaagcgg cgtcagacat ttatgcgcca
    31381 gtaagcggtg aaatcgtggc ggtaaacgac gcactgagcg attccccgga actggtgaac
    31441 agcgaaccgt atgcaggcgg ctggatcttt aaaatcaaag ccagcgatga aagcgaactg
    31501 gaatcactgc tggatgcgac cgcatacgaa gcattgttag aagatgagta acggctttat
    31561 tcctcttctg cgggagagga tcagggtgag gaaaatttat gcctcaccct cactctcttc
    31621 gtaaggagag aggttcacaa ttcactgcac gtttcaggaa ccatcgctca tgacacagac
    31681 gttaagccag cttgaaaaca gcggcgcttt tattgaacgc catatcggac cggacgccgc
    31741 gcaacagcaa gaaatgctga atgccgttgg tgcacaatcg ttaaacgcgc tgaccggcca
    31801 gattgtgccg aaagatattc agcttgcgac accaccgcag gttggcgcac cggcgaccga
    31861 atacgccgca ctggcagaac tcaaggctat tgccagtcgc aataaacgct tcacgtctta
    31921 catcggcatg ggttacaccg ccgtgcagct accgccggtt atcctgcgta acatgctgga
    31981 aaatccgggc tggtataccg cgtacactcc gtatcagcct gaagtctccc agggccgcct
    32041 tgaagcactg ctcaacttcc agcaggtaac gctggatttg actggactgg atatggcctc
    32101 tgcttctctt ctggacgagg ccaccgctgc cgccgaagca atggcgatgg cgaaacgcgt
    32161 cagcaaactg aaaaatgcca accgcttctt cgtggcttcc aatgtgcatc cgcaaacgct
    32221 ggatgtggtc cgtactcgtg ctgaaacctt tggttttgaa gtgattgtcg atgacgcgca
    32281 aaaagtgctc gaccatcagg acgtcttcgg cgtgctgtta cagcaggtag gcactaccgg
    32341 tgaaattcac gactacactg cgcttattag cgaactgaaa tcacgcaaaa ttgtggtcag
    32401 cgttgccgcc gatattatgg cgctggtgct gttaactgcg ccgggtaaac agggcgcgga
    32461 tattgttttt ggttcggcgc aacgcttcgg cgtgccgatg ggctacggtg gcccacacgc
    32521 ggcattcttt gcggcgaaag atgaatacaa acgctcaatg ccgggccgta ttatcggtgt
    32581 atcgaaagat gcagctggca ataccgcgct gcgcatggcg atgcagactc gcgagcaaca
    32641 tattcgtcgt gagaaagcga actccaacat ttgtacttcc caggtactgc tggcaaacat
    32701 cgccagcctg tatgccgttt atcacggccc ggttggcctg aaacgtatcg ctaaccgcat
    32761 tcaccgtctg accgatattc tggcggcggg cctgcaacaa aaaggtctga aactgcgcca
    32821 tgcgcactat ttcgacacct tgtgtgtgga agtggccgac aaagcgggcg tactgacgcg
    32881 tgccgaagcg gctgaaatca acctgcgtag cgatatcctg aacgccgtgg ggatcaccct
    32941 tgatgaaacc accacgcgtg aaaacgtaat gcagcttttc agtgtgctgc tgggcgataa
    33001 ccacgggctg gagatcgaca cgctggacaa agacgtggct cacgacagcc gctctatcca
    33061 gcctgcgatg ctgcgcgacg acgaaatcct cacccatccg gtgtttaatc gctatcacag
    33121 cgaaaccgaa atgatgcgct atatgcactc gctggagcgt aaagatctgg cgctgaatca
    33181 ggcgatgatc ccgctgggtt cctgcaccat gaaactgaac gccgccgccg agatgatccc
    33241 aatcacttgg acggaatttg ccgaactgca cccgttttgc ccgccggaac aggctgaagg
    33301 ttatcagcag atgattgcgc agctggctga ctggctggtg aaactgaccg gttacgacgc
    33361 cgtttgtatg cagccgaact ccggcgcaca gggcgaatac gcgggcctgc tggcgattcg
    33421 tcattatcat gaaagccgca acgaagggca tcgcgatatc tgcctgatcc cggcttctgc
    33481 gcacggaact aaccccgctt ctgcacatat ggcaggaatg caggtggtgg ttgtggcgtg
    33541 tgataaaaac ggcaacatcg atctgactga tctgcgcgcg aaagcggaac aggcgggcga
    33601 taacctctcc tgtatcatgg tgacttatcc ttctacccac ggcgtgtatg aagaaacgat
    33661 ccgtgaagtg tgtgaagtcg tgcatcagtt cggcggtcag gtttaccttg atggcgcgaa
    33721 catgaacgcc caggttggca tcacctcgcc gggctttatt ggcgcggacg tttcgcacct
    33781 caacctgcat aaaactttct gcattccgca cggcggtggt ggtccgggta tgggaccgat
    33841 cggtgtgaaa gcgcatctgg caccgtttgt accgggtcat agcgtggtgc aaatcgaagg
    33901 catgttaacc cgtcagggcg cagtttctgc ggcaccgttc ggtagcgcct ctatcctgcc
    33961 aatcagctgg atgtacatcc gcatgatggg cgcagaaggg ctgaaaaaag caagccaggt
    34021 ggcaatcctc aacgccaact atattgccag ccgcctgcag gatgccttcc cggtgctgta
    34081 taccggtcgc gacggtcgcg tggcgcacga atgtattctc gatattcgcc cgctgaaaga
    34141 agaaaccggc atcagcgagc tggatattgc caagcgcctg atcgactacg gtttccacgc
    34201 gccgacgatg tcgttcccgg tggcgggtac gctgatggtt gaaccgactg aatctgaaag
    34261 caaagtggaa ctggatcgct ttatcgacgc gatgctggct atccgcgcag aaattgacca
    34321 ggtgaaagcc ggtgtctggc cgctggaaga taacccgctg gtgaacgcgc cgcacattca
    34381 gagcgaactg gtcgccgagt gggcgcatcc gtacagccgt gaagttgcgg tattcccggc
    34441 aggcgtggca gacaaatact ggccgacggt gaaacgtctg gatgatgttt acggcgaccg
    34501 taacctgttc tgctcctgcg taccgattag cgaataccag taattcactg attcgagtat
    34561 tttctaaagg cgcttcggcg cctttttagt cagatgacaa agtacaaaag tgctcagaca
    34621 gtcccctcgc ccctttgggg agagggttag ggtgagggga acaggccggc actggcgcga
    34681 atatttaccc tcaccccgac cctctccctg aaagggcgag ggggaaaagc gtgccaacat
    34741 tgaagattga gccagtttgt tagcaatctc aaagatacgt caacgaatta atttttctcg
    34801 gaaaaacaaa tggctatagc acttgtgacc ggtggcagtc gcggcatcgg gcgggcaact
    34861 gcattactgt tggcgcaaga agggtatacg gtggcggtta attatcagca aaacctccac
    34921 gcggcgcagg aagtgatgaa cttaataatg caagccggtg gcaaagcatt cgtgctccag
    34981 gcggatatca gcgacgaaaa ccaggtcgtt gcgatgttta cagcaatcga tcagcacgat
    35041 gaaccgctag cagcgctggt caataacgcc gggatcttgt ttacccagtg caccgttgaa
    35101 aaccttaccg cagagcgaat caaccgagta ctttccacca acgtgacggg atattttctc
    35161 tgctgccgcg aggcggtaaa acgcatggcg cttaaaaatg gtggcagtgg cggcgctatc
    35221 gtcaatgtct cttcggtggc ctcacggttg ggttcgccag gggaatatgt tgattacgcg
    35281 gcatcgaaag gggcgattga ttcgttaacc accggactat cgctggaagt cgccgcgcag
    35341 gggatccgcg ttaactgcgt gcggccaggg tttatttata ccgaaatgca cgccagcgcg
    35401 gcgagcctgg acgcgtcgat cgcgttaagt cgaacatccc catgcagcgt ggtggacagg
    35461 cagaagaggt cgcgcaggcc attgtctggc tactaagtga taaagcctct tacgtcacgg
    35521 gaagttttat cgatttggcg ggcgggaaat aaaacaggga agttgtctga ccggatgcaa
    35581 caagtattgc atccggtact tcatcgactt aaagcttctc gccgttgctg gcaatcacct
    35641 ctttgtacca gttaaagctc ttcttacgtg aacgcgacat atcgccagta ccgtcgtcat
    35701 gtttattcac atagataaag ccgtagcgtt tgctgtactg cccggtagtg aatgacacgc
    35761 agtcgatgca gccccacggt gtgtagccca tcagatccac accatcgtaa gtcactgctt
    35821 ttttcatctc ttcaatatgg gcgcgcaggt agtcaatgcg gtagtcgtcg ttgatgctgc
    35881 catcttcttc cactttgtcg taagcgccaa aaccgttttc gacaataaac agcggcctct
    35941 gataacgctc atacagttcg caaagggcat agcgcagacc tactggatca atctgccagc
    36001 cccagtcaga cgctttaaca tacgggttcg gtacgctgcc ttcaaaacca gagatcgcat
    36061 cgccggtgcc gccttcggcc ttcactgcgt tggtcatgta atagctgaaa ccaagataat
    36121 cgcaggtgcc ttcgcgcagt acatcgagat cgccgtcttc cattttgata ttaaatccgc
    36181 gacgttccca ctcgttcaat acataggacg ggtaatagcc gcatagctgc acatcggtaa
    36241 agacgtagcg ttcgcgcatc gactcctgag cgaacatcac atcgtccggg ttacatgagt
    36301 agggatagag cggcaccatc gccagcatac agccgacttt catctccggg ttaatgcgac
    36361 gcgcggcttt cactgccagg gcgctggcga caaactggtg atgcagcacc tggtacatcg
    36421 tctcttccgg gttgtcatgc tcggtataca ccacgccgga gcagcagtaa ccaaacagcg
    36481 gcgcacgcca gttacgctgg ttgtttattt cgttgaaggt catccagtat ttgactttat
    36541 gcttgtagcg ttcgaatacc acttccgcga aacggacaaa gaaatcaacg aatttacggt
    36601 tggtccagct accgtattgc tgcaccagat gcagcggcat ttcaaagtgg gagagggtta
    36661 tcaccggttc gatgttgtat ttcagcagtt catcgaacag agagtcgtaa aacttcagcc
    36721 cttcttcgtt tggctgagct tcatcacctt ttggaaaaat acgcgtccag gcgatggaag
    36781 tccgaaaaca tttgaagccc atttcggcaa atagcttgat gtcttccttg tagtgaccat
    36841 aaaaatcaac ggcttcatgg tttggatagt attttcctgg caagacttct ttggtgattt
    36901 cgcgcggcac gccgtgtgcg ccaccggtca gaacgtcaca aatgctcggt cctttgccgc
    36961 ctttgttcca gccgccttcg acctgatgag cggcaacggc gccaccccat aagaaatctt
    37021 tcggtaaggt gagttttttc actatcatag gctccagatc agtcaattct tacaggaaat
    37081 agtaccatgc agccaaacga catcactttt ttcaacgttt ccaggatgac attctggctg
    37141 ggcgtaaaac catcaccatc cgcgacgagt ctgaatcgca cttcaaaacg ggtgatgtgc
    37201 ttcgtgtcgg acgttttgaa gatgacggtt atttttgcac gattgaagtc accgcaacct
    37261 caaccgtaac gctggataca ctgacggaaa aacatgcaga gcaggaaaat atgaccctgc
    37321 ctgaactgaa aaaggtcatt gctgacatct atcccggtca gacacaattt tatatgattg
    37381 aatttaaatg tctttaaatt gaactcacgc ctgttagttg aaatttcgca tttaatttca
    37441 tgatttttag gagattagtc tcaaatcaga atttatttta gctaacaggt gttcactgga
    37501 actattctca gttacgctgg agaggtatac atgtgcgtgt acgtttctct ggagtaagtt
    37561 atggttcaga agcccctcat taagcaggga tattcgctgg cagaggaaat tgccaacagc
    37621 gtcagtcacg gcattgggct ggtgtttggt atcgttgggc tggtgttgct actggttcag
    37681 gcggtggatc tcaatgccag cgccacagcg ataaccagtt acagcctcta tggcggcagt
    37741 atgatcctgc tgttcctcgc ttcgacgctc tatcacgcca ttcctcatca acgggcaaaa
    37801 atgtggctga agaaatttga ccattgcgct atttacctgt tgattgccgg aacctacacg
    37861 ccgtttttgc tggtggggct ggattctccg ttagcgcgcg ggttgatgat tgttatctgg
    37921 agcctagcat tgctggggat tctgttcaaa ctgaccatcg cgcaccgatt caaaatttta
    37981 tctctggtga cttatctggc gatgggctgg ctgtcgctgg tggtaattta tgaaatggca
    38041 gttaagctcg cggcgggcag cgttacctta ctggcggtag gcggcgtggt ttattcgctc
    38101 ggggtgattt tctacgtctg caaacgcatt ccatacaacc atgccatctg gcacggcttc
    38161 gtgctcggcg gtagtgtgtg ccactttctg gcgatctatt tgtatattgg gcaggcgtaa
    38221 tgggtatgcc tttgtaggcc tgataagacg cgtcagcgtc gcatcaggca tcggagcgca
    38281 accgccggat gcggcgtgaa cgccttatcc gatctacggg cctcgtccct gttgtgataa
    38341 aggcatcaaa tcccagacat gataagacgc gtcagcgtcg catcaggcga taaatgcctg
    38401 atgcgataca gagatttact cttcgagcga atacggcagc ggctcgatat gcagtgtatt
    38461 cgcatcgtcg cgtacgcgga agatgctgtc cggttccata tcgttattca tgacgacctg
    38521 taccacgacc tgaccatctt ccagttttac cgcagccagc accgtaccgg tacgacgcca
    38581 gttctcgccc attttcagct ctaagtcttc accagcttcc ggcagtcggc tgacactacc
    38641 tgccagcaac cagagcgcgc gtttgttggc accacggaat tttgctcgcg ccaccatctc
    38701 ttgtccggta taacagcctt tcttaaagct gataccgccc agcgcctgga gattggtcgc
    38761 ctgtgggata aactgcccgc tgttggcggc atcaatcacc gggaaaccag cttcaatgtt
    38821 taatgccagc cactgttggc tattgttcag ttccgcttca ccgcgcagtt tatcggtcag
    38881 catattagca gtagcttcat cggttacgat caggaaacgt tctgccgggt gttcaaacca
    38941 tagcaaagtg gtcgcgcctt ctttgactac ctgtttttct ttcgaaggca gttcgctaaa
    39001 gagatttgcc agcgcggcgc gcgcctgaaa accggcaaca ccaagcagca cacgctcgtc
    39061 gtctggcgcg atggtcactt tagagaatac cgcatatttt ttcagttcag tcagctgcgg
    39121 ttcacgcacg ctgcgccgtt caatccatgc aaagccatcg ccgtcgcgga acagacgtaa
    39181 attgctccac attttacctt tggcgtcgca atgggcggcg agcaggtgct gatcttctgc
    39241 catctggctg acatctgctg tcacctgacc ctgcatatat ttttcgctgt ccgcgccagt
    39301 aatggtggca agcgcccagt catcaagcgt catcagcgtc agcggtaaac gggcagaagc
    39361 cgtaggctga cgtggaggaa aaggtgtaaa agccataata gagtcctgag ttgcttaacg
    39421 gcgtattaat gactaatggt aaaagagcta tgaggcaatg caagtgcttt cactacgcga
    39481 tctgtgcctt ctgcgaggcg actgccaaaa aaatgaagcc cttgaattgt tgtgaattaa
    39541 tttcgcgatc gcgatggtgt ttactgatat tcccgtaaaa gaagtcgaaa aacacgttac
    39601 aatagccggg tactggattt tcgtgagaca ggaagaagat ggacattaac aacaaagccc
    39661 gaattcattg ggcatgccgc cgtggtatgc gcgaactcga tatttcaatc atgccgtttt
    39721 tcgaacatga gtacgacagc ttaagcgatg acgaaaaacg catctttatt cgtctgctgg
    39781 aatgtgacga tccggacctg tttaactggc tgatgaatca cggtaaaccg gccgatgcag
    39841 aactggaaat aatggtccga ctcatccaga cacggaaccg ggaacgtggt cctgtggcaa
    39901 tctgatttgc gcgtctcctg gcgcgcacag tggctttcct tgctgattca tgggctggtt
    39961 gccgctgtta ttttactcat gccctggccg ctcagttaca ccccgttatg gatggtgtta
    40021 ctttcgctgg tggtatttga ttgcgttcgc agccagcggt gtattaatgc tcgccagggg
    40081 gaaattcgct tgttgatgga cgggcgtttg cgttggcaag ggcaggagtg gagcatcgtc
    40141 aaagcaccgt ggatgattaa gagcggcatg atgctgcgtt tacgttctga tggcggtaaa
    40201 cggcaacatt tatggctggc agccgacagc atggacgaag ccgaatggcg ggatttacgg
    40261 cggattttgt tgcaacaaga gacgcaaaga taaagatgac ggcaaagcat atgcgctaag
    40321 ccgtcagtga gatcaggcgt aatgctctgc catttcgttg aggatttgct cgcaccagct
    40381 ctgaatacgc tcgtcgctaa ggtcatactg gttagtttca tccagcgcca gacccacgaa
    40441 cagttgcccg tcagcaatca ccggtttcgg gctggtaaat tcatatcctt ccgttggcca
    40501 gtagccgacg aacttcacgc ctttggtcga gagtttgtca tgcagcatac cgagcgcatc
    40561 gaggaaccac tcgccgtatc ccagttgatc gccaagccca tacagcgcaa taattttacc
    40621 ttcaaggttc aggtcgtcga gctgatccca gacggcttcc cagtcttcct ggatttcacc
    40681 aaaatcccag gtcgggatac ccagaatgag cacatcgtac tgctccatta atttcgggga
    40741 gtcgtccttg aggttatgta aggtcaccag ttctgggccg ataatatcgc ggattttttc
    40801 tgccgccatt tcggtgtaac aggtgctgga accgtaaaaa agacccatat tcataacgta
    40861 agcttctcga ttctgctgtg gcttttgcag ctagtgtatc agaatcgctt tagtcacggc
    40921 acaataaggc ataatggatc aaatgagtga ccataagggg cgcaagtgaa acaggagctg
    40981 gcacgcatcg agcagtttct tgatgctctg tggctggaaa aaaatctggc tgaaaatacg
    41041 ttgaacgctt accgtcgcga tctgtcaatg atggtggagt ggttgcatca ccgcgggttg
    41101 acgctggcga cggcgcaaag tgacgatttg caggcattac tggcagaacg gctggagggc
    41161 gggtataaag ccaccagctc agcgcgtttg ctgagtgcag tgcgccgatt gttccagtat
    41221 ctttatcgcg aaaagtttcg tgaagacgat cccagtgcgc atctcgcttc accgaaattg
    41281 ccccagcgtt tgccaaaaga tttaagcgaa gcgcaggtcg aacgtttatt acaggcgcca
    41341 ttaattgatc agccactgga gctacgcgat aaagccatgc ttgaagtgtt gtatgctacc
    41401 gggctgcgtg tctctgaact ggtcgggctg acaatgagtg atatcagcct gcgtcagggc
    41461 gtagtgcggg tcattggtaa aggcaacaaa gagcgcctgg tgccgttagg ggaagaggcg
    41521 gtttactggc tggaaaccta tctggaacat gggcgtccgt ggctgctgaa tggtgtgtca
    41581 attgacgtgt tgtttcccag ccagcgtgcg cagcagatga cgcgacagac cttctggcac
    41641 cgcattaaac attatgccgt gctggcgggt atcgacagcg aaaagctgtc accgcatgtg
    41701 ttgcgtcacg cttttgccac tcatttatta aatcatggtg cggatttacg cgtggtgcag
    41761 atgctactgg gccacagcga tctttccacc acgcaaattt atacgcatgt cgctaccgag
    41821 cgtctgcggc aacttcatca acagcatcac ccgcgggcgt gatgtctgaa aagaacggga
    41881 agatttatga agaaaggttt tatgttgttt actttgttag cggcgttttc aggctttgct
    41941 caggctgatg acgcggcaat tcaacaaacg ttagccaaaa tgggcatcaa aagcagcgat
    42001 attcagcccg cgcccgtagc tggcatgaag acagttctga ctaacagcgg tgtgttgtac
    42061 atcaccgatg atggtaaaca tatcattcag gggccaatgt atgacgttag tggcacggct
    42121 ccggtcaatg tcaccaataa gatgctgtta aagcagttga atgcgcttga aaaagagatg
    42181 atcgtttata aagcgccgca ggaaaaacac gtcatcaccg tgtttactga tattacctgt
    42241 ggttactgcc acaaactgca tgagcaaatg gcagactata acgcgctggg gatcaccgtg
    42301 cgttatcttg ctttcccgcg ccaggggctg gacagcgatg cagagaaaga aatgaaagct
    42361 atctggtgtg cgaaagataa aaacaaagcg tttgatgatg tgatggcagg taaaagcgtc
    42421 gcaccagcca gctgcgacgt ggatattgcc gaccattacg cacttggcgt ccagcttggc
    42481 gttagcggta ctccggcagt tgtgctgagc aatggcacac ttgttccggg ttaccagccg
    42541 ccgaaagaca tgaaagaatt cctcgacgaa caccaaaaaa tgaccagcgg taaataattc
    42601 gcgtgaaaca acagatacaa cttcgtcgcc gtgaagtcga tgaaacggca gacttgcccg
    42661 ctgaattgcc tcccttgctg cgccgtttat acgccagccg gggagtacgc agtgcgcaag
    42721 aactggaacg cagtgttaaa ggtatgttgc cctggcagca actaagcggt gtcgaaaagg
    42781 ccgttgagat cctttacaac gcttttcgcg aaggaacgcg gattattgtg gtcggtgatt
    42841 tcgacgccga cggcgcgacc agcacggctc taagcgtgct ggcgatgcgc tcgcttggtt
    42901 gcagcaatat cgactacctg gtaccaaacc gtttcgaaga cggttacggc ttaagcccgg
    42961 aagtggtcga tcaggcccat gcccgtggcg cgcagttaat tgtcacggtg gataacggta
    43021 tttcctccca tgcgggagtt gagcacgctc gctcgttggg catcccggtt attgttaccg
    43081 atcaccattt gccgggcgac acattacccg cagcggaagc gatcattaac cctaacttgc
    43141 gcgactgtaa tttcccgtcg aaatcactgg caggcgtggg tgtggcgttt tatctgatgc
    43201 tggcgctgcg cacctttttg cgcgatcagg gctggtttga tgagcgcggc atcgcaattc
    43261 ctaacctggc agaactgctg gatctggtag cactggggac agtagcggac gtcgtgccgc
    43321 tggacgctaa taatcgcatt ctgacctggc aggggatgag tcgcatccgt gccggaaagt
    43381 gccgtccagg gattaaagcg ctgctggaag tggcaaatcg cgatgcacaa aaactagccg
    43441 ccagcgattt aggttttgcg ctgggaccgc gtctcaatgc tgccgggcga ctggatgata
    43501 tgtccgttgg tgtggcgctg ttgctgtgcg acaacatcgg cgaagcgcgc gtgctggcaa
    43561 atgaactcga tgcgctaaac cagacgcgaa aagagatcga acaaggaatg caggttgaag
    43621 ccctgaccct gtgcgagaaa ctggagcgca gccgtgacac gctacccggc gggctggcaa
    43681 tgtatcaccc cgaatggcat cagggcgttg tcggtattct ggcttcgcgt atcaaagagc
    43741 gttttcaccg tccggttatc gcctttgcgc cagcaggtga cggtacgctg aaaggttccg
    43801 gtcgctccat tcaggggctg catatgcgtg atgcgctgga gcgattagac acactctacc
    43861 ccggcatgat gctcaagttt ggcggtcatg cgatggcggc gggtttgtcg ctggaagagg
    43921 ataaattcga actctttcaa caacggtttg gcgaactggt tactgagtgg ctggcccctt
    43981 cgctattgca aggcgaagtg gtgtcagacg gtccgttaag cccggccgaa atgaccatgg
    44041 aagtggcgca gctgctgcgc gatgctggcc cgtgggggca gatgttcccg gagccgctgt
    44101 ttgacggtca tttccgtctg ctgcaacagc ggctggtggg cgaacgtcat ttgaaggtga
    44161 tggtcgaacc ggtcggcggc ggtccactgc tggatggtat tgcttttaat gtcgataccg
    44221 ctctctggcc ggataacggc gtgcgcgaag tgcaactggc ttataagctc gatatcaacg
    44281 agtttcgcgg caaccgcagc ctgcaaatta tcatcgacaa tatctggcca atttagcgtc
    44341 atcttctcta taaaaaagag cgtggattgg gtacaatccc gctcttatca ccgcattttg
    44401 actagctcaa taaaagaaat cagaccatgt ttgaaattaa tccggtaaat aatcgcattc
    44461 aggacctcac ggaacgctcc gacgttctta gggggtatct ttgactacga cgccaagaaa
    44521 gagcgtctgg aagaagtaaa cgccgagctg gaacagccgg atgtctggaa cgaacccgaa
    44581 cgcgcacagg cgctgggtaa agagcgttcc tccctcgaag ccgttgtcga caccctcgac
    44641 caaatgaaac aggggctgga agatgtttct ggtctgctgg aactggctgt agaagctgac
    44701 gacgaagaaa cctttaacga agccgttgct gaactcgacg ccctggaaga aaaactggcg
    44761 cagcttgagt tccgccgtat gttctctggc gaatatgaca gcgccgactg ttacctcgat
    44821 attcaggcgg ggtctggcgg tacggaagca caggactggg cgagcatgct tgagcgtatg
    44881 tatctgcgct gggcagaatc gcgtggtttc aaaactgaaa tcatcgaaga gtcggaaggt
    44941 gaagtggcgg gtattaaatc cgtgacgatc aaaatctccg gcgattacgc ttacggctgg
    45001 ctgcgtacag aaactggcgt tcaccgcctg gtgcgtaaaa gcccgtttga ctccggcggt
    45061 cgtcgccaca cgtcgttcag ctctgcgttt gtttatccgg aagttgatga tgatattgat
    45121 atcgaaatca acccggcgga tctgcgcatt gacgtttatc gcgcgtccgg cgcgggcggt
    45181 cagcacgtta accgtaccga atctgcggtg cgtattaccc acatcccgac cgggatcgtg
    45241 acccagtgcc agaacgaccg ttcccagcac aagaacaaag atcaggccat gaagcagatg
    45301 aaagcgaagc tttatgaact ggagatgcag aagaaaaatg ccgagaaaca ggcgatggaa
    45361 gataacaaat ctgacatcgg ctggggcagc cagattcgtt cttatgtcct tgatgactcc
    45421 cgcattaaag atctgcgcac cggggtagaa acccgcaaca cgcaggccgt gctggacggc
    45481 agcctggatc aatttatcga agcaagtttg aaagcagggt tatgaggaac caacatgtct
    45541 gaacaacacg cacagggcgc tgacgcggta gtcgatctta acaatgaact gaaaacgcgt
    45601 cgtgagaagc tggcgaacct gcgtgagcag ggaattgcct tcccgaacga tttccgtcgc
    45661 gatcatacct ctgaccaatt gcacgcagaa ttcgacggta aagagaacga agaactggaa
    45721 gcgctgaaca tcgaagtcgc cgttgctggc cgcatgatga cccgccgtat tatgggtaaa
    45781 gcgtctttcg ttaccctgca ggatgtgggt ggccgcattc agctgtacgt tgcccgtgac
    45841 gatctgccgg aaggcgttta caacgagcag ttcaaaaaat gggacctcgg cgacatcctc
    45901 ggcgcgaaag gtaaactgtt caaaaccaaa accggcgaac tgtctatcca ctgtaccgag
    45961 ctgcgtctgc tgaccaaagc actgcgtccg ctgccggata aattccacgg cttgcaggat
    46021 caggaagcgc gctatcgtca gcgttatctg gatctcatct ctaacgatga atcccgtaac
    46081 acctttaaag tacgctcgca gatcctctct ggtattcgcc agttcatggt gaaccgcggc
    46141 tttatggaag ttgaaacgcc gatgatgcag gtgatcccag gcggtgccgc tgcgcgtccg
    46201 tttatcaccc accataacgc gctggatctc gacatgtacc tgcgtatcgc gccggaactg
    46261 tacctcaagc gtctggtggt cggtggcttc gagcgtgtat tcgaaatcaa ccgtaacttc
    46321 cgtaacgaag gtatttccgt acgtcataac ccagagttca ccatgatgga actctacatg
    46381 gcttacgcag attacaaaga tctgatcgag ctgaccgaat cgctgttccg tactctggca
    46441 caggatattc tcggtaagac ggaagtgacc tacggcgacg tgacgctgga cttcggtaaa
    46501 ccgttcgaaa aactgaccat gcgtgaagcg atcaagaaat atcgcccgga aaccgacatg
    46561 gcggatctgg ataacttcga ctctgcgaaa gcgattgctg aatctatcgg tatccacgtt
    46621 gagaagagct ggggtctggg ccgtatcgtt accgagatct tcgaagaagt ggcagaagca
    46681 catctgattc agccgacctt cattactgaa tatccggcag aagtttctcc gctggcgcgt
    46741 cgtaacgaca ttaacccgga aatcacagac cgctttgagt tcttcatcgg tggtcgtgaa
    46801 atcggtaacg gctttagcga gctgaatgac gcggaagatc aggcgcaacg cttcctggat
    46861 caggttgccg cgaaagacgc aggtgacgac gaagcgatgt tctacgatga agattacgtt
    46921 accgcactgg aacatggctt accgccgaca gcaggtctgg ggattggcat cgaccgtatg
    46981 gtaatgctgt tcaccaacag ccataccatc cgcgacgtta ttctgttccc ggcgatgcgt
    47041 ccggtgaaat aagcattacg ttatgctcac aaccccggca aatgtcgggg ttttttattt
    47101 aagctgggta aatgcagata atcgtattct ggcttcgcga tttgtcgcct gcatcaccat
    47161 ccacggactg aacgcccacg gcgtggcatc aataccgcgt aatacatcag ctaaatcaca
    47221 ccattgataa tccatcactt catcatcgtt gatctgtaac gcactggtgg tgcgtgcggc
    47281 aaataccgga cacacttcat tttccacaat gcctctcgga tcggtggcgc ggtagcgaaa
    47341 gtcaggatag atagattcag gaggcgtaat ttccacgcca agctcataac ggcaacggcg
    47401 gatcaccgcg tcttcgctgc tttctcccag ttgtgggtgc ccacaaaccg agttagtcca
    47461 cacgccaggc cacgcttttt tgctcagtgc gcggcgggta actaataatt gccccttggc
    47521 attaaacagc caactggaga acgcgagatg taagagggtg tctgccgtgt gtgcggcata
    47581 cttttccagc gtacccgtgg gaactccctg tgcattcaat aaaatgacgt gttccgtttg
    47641 cataatttct cacatgtaat tctgatcact catcattata gataattgaa gagtgaaatg
    47701 attaaccaag agagcgttta tcgacaaccg cccgttttga gcggatgccg acaggtaaaa
    47761 accatttaaa aaagtagcat catatatttg aagcgacttt caatggccat tgcaacacag
    47821 tcagaaatga caggaataga gagagtaaaa agcaacaaca gactgaatat agcgcgcatt
    47881 aaaaaattca tattattctc catgctcatt tttcttcagc ttgcgcatca gaagccacat
    47941 acgtactgtc ctgacttcta aatctttatc agagacggat tccttcacca ggtcagcatg
    48001 atgctgatag ccattaaaga agacgttcag cacaacggcg ctaagggttg cgagcataat
    48061 gccgctatgt agcagcggtt gcagtacggc cggtaactta gaaaagaaat cgtgagagag
    48121 cgtcggagtc atgccaacgc cgagactgat cgccacaata tagaggttgt aacggttggt
    48181 ggtgtagtta cagcgcgaca gaattcgaat ccctgtcgcc agtaccatgc cgaacatcac
    48241 tagaccagcg ccgcccagca caaattgcgg aatggaggct accagcaccg ccatttttgg
    48301 caccatgcca aacaggatta atataattcc cgaagaaata cacacccagc ggctatgaac
    48361 gcgcatcacg ctaaccaggc caacgttttg agaaaatgac gtgtggggga agctgttaaa
    48421 cgtgccgcct atcattgtcc ctacgccatc gacacgcagc ccgcaaataa tatcgtgtga
    48481 agagagttta cgaccgacta tttcacccag tgccaggaac atccccatcg actcgataaa
    48541 cacgatga
//
LOCUS       DAJVRP010000020        48311 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVRP010000020 DAJVRP010000000
VERSION     DAJVRP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.020"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            187..480
                     /locus_tag="OQN12_001130"
     CDS             187..480
                     /locus_tag="OQN12_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1653543.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OQN12_001131"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OQN12_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCS1653544.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="OQN12_001132"
     CDS             complement(1922..2374)
                     /locus_tag="OQN12_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS1653545.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OQN12_001133"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OQN12_001133"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1653546.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="OQN12_001134"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="OQN12_001134"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS1653547.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OQN12_001135"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OQN12_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1653548.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OQN12_001136"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OQN12_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1653549.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OQN12_001137"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OQN12_001137"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1653550.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OQN12_001138"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OQN12_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1653551.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OQN12_001139"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OQN12_001139"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1653552.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OQN12_001140"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OQN12_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS1653553.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OQN12_001141"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OQN12_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1653554.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="OQN12_001142"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="OQN12_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1653555.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="OQN12_001143"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="OQN12_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1653556.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="OQN12_001144"
     CDS             complement(15085..15582)
                     /locus_tag="OQN12_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1653557.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="OQN12_001145"
     CDS             complement(15579..15941)
                     /locus_tag="OQN12_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1653558.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="OQN12_001146"
     CDS             complement(15931..16278)
                     /locus_tag="OQN12_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1653559.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="OQN12_001147"
     CDS             16387..16800
                     /locus_tag="OQN12_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653560.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="OQN12_001148"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="OQN12_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="OQN12_001149"
     CDS             complement(18324..20039)
                     /locus_tag="OQN12_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCS1653561.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="OQN12_001150"
     CDS             20206..21099
                     /locus_tag="OQN12_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1653562.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="OQN12_001151"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="OQN12_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23527)
                     /locus_tag="OQN12_001152"
                     /pseudo
     CDS             complement(21910..23527)
                     /locus_tag="OQN12_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23822..24496
                     /locus_tag="OQN12_001153"
     CDS             23822..24496
                     /locus_tag="OQN12_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1653563.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26196)
                     /locus_tag="OQN12_001154"
     CDS             complement(24535..26196)
                     /locus_tag="OQN12_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1653564.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="OQN12_001155"
     CDS             complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="OQN12_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1653565.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="OQN12_001156"
     CDS             complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="OQN12_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1653566.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27339..27411)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27650..27982
                     /locus_tag="OQN12_001157"
     CDS             27650..27982
                     /locus_tag="OQN12_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1653567.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27984..29204)
                     /locus_tag="OQN12_001158"
     CDS             complement(27984..29204)
                     /locus_tag="OQN12_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS1653568.1"
                     /translation="MKQITFAPRNHLLTNTNTNTWTPDSQWLVFDVRPSGASFTGETI
                     ERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVI
                     AEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLR
                     NVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHAL
                     AFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTF
                     THHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTD
                     IQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLA
                     WMEGGQLWITETDR"
     gene            29305..30405
                     /gene="cbrA"
                     /locus_tag="OQN12_001159"
     CDS             29305..30405
                     /gene="cbrA"
                     /locus_tag="OQN12_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCS1653569.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30366..31703)
                     /locus_tag="OQN12_001160"
     CDS             complement(30366..31703)
                     /locus_tag="OQN12_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1653570.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31778..32926)
                     /gene="dgoD"
                     /locus_tag="OQN12_001161"
                     /pseudo
     CDS             complement(31778..32926)
                     /gene="dgoD"
                     /locus_tag="OQN12_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32923..33540)
                     /gene="dgoA"
                     /locus_tag="OQN12_001162"
     CDS             complement(32923..33540)
                     /gene="dgoA"
                     /locus_tag="OQN12_001162"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCS1653571.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33524..34402)
                     /gene="dgoK"
                     /locus_tag="OQN12_001163"
     CDS             complement(33524..34402)
                     /gene="dgoK"
                     /locus_tag="OQN12_001163"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCS1653572.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34399..35088)
                     /gene="dgoR"
                     /locus_tag="OQN12_001164"
     CDS             complement(34399..35088)
                     /gene="dgoR"
                     /locus_tag="OQN12_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCS1653573.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35366..36022
                     /locus_tag="OQN12_001165"
     CDS             35366..36022
                     /locus_tag="OQN12_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653574.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36068..36880)
                     /gene="yidA"
                     /locus_tag="OQN12_001166"
     CDS             complement(36068..36880)
                     /gene="yidA"
                     /locus_tag="OQN12_001166"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCS1653575.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36994..37392)
                     /locus_tag="OQN12_001167"
     CDS             complement(36994..37392)
                     /locus_tag="OQN12_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCS1653576.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37632..40046)
                     /gene="gyrB"
                     /locus_tag="OQN12_001168"
     CDS             complement(37632..40046)
                     /gene="gyrB"
                     /locus_tag="OQN12_001168"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1653577.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40075..41148)
                     /gene="recF"
                     /locus_tag="OQN12_001169"
     CDS             complement(40075..41148)
                     /gene="recF"
                     /locus_tag="OQN12_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1653578.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41148..42248)
                     /gene="dnaN"
                     /locus_tag="OQN12_001170"
     CDS             complement(41148..42248)
                     /gene="dnaN"
                     /locus_tag="OQN12_001170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1653579.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42253..43656)
                     /gene="dnaA"
                     /locus_tag="OQN12_001171"
     CDS             complement(42253..43656)
                     /gene="dnaA"
                     /locus_tag="OQN12_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1653580.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43953..44033
                     /locus_tag="OQN12_001172"
     CDS             43953..44033
                     /locus_tag="OQN12_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653581.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44263..44403
                     /gene="rpmH"
                     /locus_tag="OQN12_001173"
     CDS             44263..44403
                     /gene="rpmH"
                     /locus_tag="OQN12_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1653582.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44420..44779
                     /gene="rnpA"
                     /locus_tag="OQN12_001174"
     CDS             44420..44779
                     /gene="rnpA"
                     /locus_tag="OQN12_001174"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1653583.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44743..45000
                     /gene="yidD"
                     /locus_tag="OQN12_001175"
     CDS             44743..45000
                     /gene="yidD"
                     /locus_tag="OQN12_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1653584.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45003..46649
                     /gene="yidC"
                     /locus_tag="OQN12_001176"
     CDS             45003..46649
                     /gene="yidC"
                     /locus_tag="OQN12_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1653585.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46755..48119
                     /gene="mnmE"
                     /locus_tag="OQN12_001177"
     CDS             46755..48119
                     /gene="mnmE"
                     /locus_tag="OQN12_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1653586.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11993 a        13059 c        12206 g        11053 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcacgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tgatgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgcagggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccacc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttgg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag
    24961 gatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc
    25021 tggcacaaag acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg
    25081 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    25141 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc
    25201 actggcgacc agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc
    25261 tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg
    25321 ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    25381 cagttgcgca ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg
    25441 gataatggta tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat
    25501 aattttgtcg ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt
    25561 ctcaacgcga atattgatag tcttgatcaa cgcgccgcca ttggtgcgtg aagactcgtg
    25621 ctgctgagct tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa
    25681 caaaatcccg caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac
    25741 catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    25801 attggttacc gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt
    25861 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    25921 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    25981 ataaacgaac aggatcaggc caaactcctg aataacatgc agcatatcgc tacttaatgt
    26041 catccccgcc tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat
    26101 acctaatcct atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa
    26161 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    26221 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    26281 tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga
    26341 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    26401 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    26461 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    26521 agccagcgta aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    26581 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat
    26641 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    26701 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    26761 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    26821 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    26881 ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat
    26941 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    27001 accatttacc aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc
    27061 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    27121 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    27181 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    27241 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    27301 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    27361 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    27421 aggcgcgtcc tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta
    27481 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
    27541 cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga
    27601 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
    27661 tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt
    27721 gatgtcccat accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
    27781 gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
    27841 taccgctgta atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt
    27901 gcgctcgcgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
    27961 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
    28021 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
    28081 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt
    28141 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
    28201 atatccgttt tgttatgggt taacttgcgc ggttcgccgc cctgtggcga gataagccac
    28261 agttgcacaa tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta
    28321 cagcgcaccc agtggcgcgg gacgttgact aaccccggat aagcccgatg atgggtaaag
    28381 gttaaacgtc gctgcacgac gccacgcggt ggcgcgggta gggttgtttc cgttccactt
    28441 aacggcgcat cacctgccga tttccagcta gcttcatctt gcggtaactc aacgataaat
    28501 agctccggca ctttctcgcc ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga
    28561 tttcctaccc atccttcttc ataagcacga ttgatttcat cactgccagg ctgcggcgtg
    28621 ggcgtggttt tactcaccag cacacaccag tggctaccgc tgtattcacg cggatgttgc
    28681 ttttgtacgt tgaccgggcc aaacggtgca gcaaccccga cgtttcgcaa atccagcgcc
    28741 ggatcgagtt gatgcattac atggtcgtta taggtaaagc tccccctttc accgttcggg
    28801 ctaaagacat gcacatggct gccaccgcgc agcgctcctg gcgtatacgg cgcggtaata
    28861 tccattgcat cgagattgct caccttgccg ccttcagcaa tgactccgcg tcgatgatgg
    28921 aagtcgtaat gccatgtttc atcaggattt tcagggccgt gaataaagac atatttctct
    28981 gacttcggat gaacggtcac cacgccgacg tgtgcgccct gtgacgcgcg atagataacc
    29041 tcgacctcgc ctgtatggat attcacacgc tcaatggtct cgccggtaaa cgacgcccca
    29101 gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt ggtattggta
    29161 ttggtgagca ggtgattacg gggagcaaag gttatctgtt tcatggcgcg gtcctgatac
    29221 aaaacggttc ccgccatcat acttcacaag gacttcactt tcagacaata gtcttttgcc
    29281 atcgtgatga atgtgagtga aaagatggaa catttcgacg tggcgattat tggcctcggc
    29341 ccggcagggt cggcgttggc acgaaagtta gccggcaaaa tgcaggtgat cgcgctggat
    29401 aaaaagcacc agtgcggtac tgaaggtttc agcaaacctt gcggcggtct gctggcaccg
    29461 gacgcgcagc gatcttttat tcgcgatgga ctgacgcttc ctgtcgatgt gatcgccaat
    29521 ccacagattt tcagcgtcaa aactgtcgac gtcgccgcat cgctcacgcg taactaccag
    29581 cgaagctata tcaatattaa tcgccatgct ttcgacttgt ggatgaaatc gctgatcccc
    29641 gccagcgttg aggtttatca cgatagcctg tgccgaaaaa tctggcgtga ggatgataaa
    29701 tggcatgtca tttttcgtgc agacggctgg gagcaacata ttaccgcccg ctatctggtc
    29761 ggtgccgatg gcgcaaactc gatggtgcgg cgacatctct acccggatca tcaaatccgt
    29821 aaatatgtcg ctatccagca gtggttcgca gagaaacatc cggtgccgtt ctactcctgc
    29881 atctttgata atgcgataac tgactgttac tcatggagta tcagcaaaga tggttatttt
    29941 atctttggcg gtgcctatcc aatgaaagac ggtcagacgc gtttcacgac gctgaaagag
    30001 aaaatgagcg cctttcagtt ccagtttggc aaagcggtga aaagtgagaa atgcacggtg
    30061 ttgtttccct cacgctggca ggattttgtc tgtggtaagg acaacgcctt tctgattggt
    30121 gaagcggcgg gatttatcag cgccagctcg ctggagggga ttagctatgc gctggatagc
    30181 gcagagattc tgcgtgcggt gttcctgaaa cagccggaga agagcaacgc cgcttactgg
    30241 cgcgccacac acaaactgcg tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg
    30301 accgcaccgg ctttaagaaa gtggattatg cgcagtggtg tggcgcatat tccacagttg
    30361 aaagattatc caacgcgctt cacatcaccc accagcagga tgtaagagag cgcgccaatc
    30421 aacgcgacgg cggagatata aaccagtgca ggcgcgaaac cgtaaccctg cgccaggtac
    30481 cccaccacca gcggaacggt gatgccgccc agaccaccgg cgaagttaaa cacgccgccg
    30541 gttaaaccaa tcaggcgcat cggtgccaga gaagagacca gcgaccaggt aatcgaagca
    30601 aaaccgttgc cgaagaatgc cagcgccatc aggcacataa tcatcatcgg atcgttggtg
    30661 tagttagcgc ccataatgca ggtggagatc agcaagccgc agataatcgg cgttttacgc
    30721 gcaaagccca gtgaaaagcc cttacgtacc agcagatccg cgacccagcc agagagcagg
    30781 acgccgacaa acgccgcgag gaatggcacc gtggtcataa agcctgcttt cagcgccgtg
    30841 attccttttt cctgggttaa atagttcggg aaccaggtta agaaaaacca cagtgcagaa
    30901 gccaccgcaa attgcccaag ataaacgccg atcagtttac gatggaacac cagtttccag
    30961 tctttggctg ttaacggctg acgcgcctct ttcttcaccg gcgcatcacc atccaccaga
    31021 ccgccgccat cacgaatgta atccagttca gctttgctga tacctttggt caggcgcggc
    31081 ggctgataaa ccttaaacca aatcagcgac cagataatgc cgataccacc agtgacaatg
    31141 aacacccagt gccagctcaa catctcctga atccagatca gcagcggagt caaaaacgcc
    31201 agaccgacaa actgaccaga cgtataaaaa ccaacggcgg aagcgcgttc atgttccggg
    31261 aaccagctgg tcaccatccg gttattggtc gggaaagcgg gcgcttcgaa aataccggtt
    31321 atcgcacgca gaccaattaa tgacattaat cccgtggcaa agccttggaa taaagtcacc
    31381 actgaccaac caaatatcgc aataaaataa gtcacgcgag aacctacgcg atctaaaaac
    31441 caaccgccgg ggatctggca tagcgtataa agccaggcga aggccgaaaa tacatagccc
    31501 atttccgctt tcgtaatgcc gaactcttcc tgaatatggg cggaagccac ggccaagtta
    31561 gcgcggtcaa cataacaaat gaccaccgta ataaagatca tcaccagcgt cagataacgc
    31621 cgacgccccg gctttgctgc attaacggga atatccatag cgagctttct ccagattttg
    31681 ggcatagcga agccgctcac catgccctgt aatttacaga gggttatatt tttgtattgc
    31741 tgttttagtg cccgatgagg ggcttacgtg gcaggaatta ccactctgct acgctgttat
    31801 cttcatgacg ccagagcgga ttacgccagt ccggggcatt tttactgaac tcaataactt
    31861 tagcttcgtc gatttccaca cctaagcccg gtttcgttaa cggtttaaag aagccgccaa
    31921 ccatgctgaa gtcttctttg tttttcacaa agtcgagtaa ctccgcgcct ttgttgtaat
    31981 gaatgcccat actttgttac tgaagtaccg cgttatagga aacaaagtcg atatgcaggc
    32041 aagccgccag tgcaatcggt ccgaacggac agtgcggcgc aagggtcaca tcataggctt
    32101 ctgccattcc agcaattttg tagcattcgg taataccgcc tgcatgggag agatccggtt
    32161 gcagaatcga aataccgcct gcctccagca cgcgtttaaa atcgaaacgt gagaacatgc
    32221 gctcacccgc cgccagtgga atatgcgttt gtgccgccaa tttcgggtag tattcggctt
    32281 gttccgccag caccggctct tcaataaaca gcgggcgata cggctccagt tctttaatca
    32341 gcactttcgc cattggcgcg ctgacgcgac cgtggaaatc aagaccaaac tcaatctgat
    32401 tgccaaaagc ttcacgaatt tgtgccacgg tgttgactgc cgcatctacc gcgcgggagt
    32461 tatcaattag ccccagttct tcacaaccgt tcagtttgaa ggtatcgaag ccgatttcgc
    32521 gcagggtttt aatgccgtcg ataacatccg ccggacgatc gccgccgacc caactgtagg
    32581 ctttaatttt gtcgcgaacc aggccgccca tcagttgcca gactggcgca ttcagcactt
    32641 tgcctttgat atcccataac gcctggtcga tcccggcgat ggcgctcatc aggattggac
    32701 cgccacgata aaatccggcg cgatacatca cttgccataa gtcgttgatg cgcgaaggat
    32761 cctgaccaat caaatagtca cccagctcgt gaactgccgc ttccaccgta cgggcgcggc
    32821 cttcaatcac gggctcgccc caaccgacca cgccttcatc ggtttcaatt ttcaggaaca
    32881 tccagcgggg aggtaaacga tacgtggtaa ttttggtgat tttcattgca ctgcctctcg
    32941 atacgcctta acaaatgctg ctgcctgctg cgcggtgcgt tctacggact gcccggcgcg
    33001 ataaagatcg ctgcctaagc ccgccccaac acaacctgcg tctatccact gcgccaggtt
    33061 ttctggcgtc acgccgccaa cggcaaagac tgcgatgtcc ggtggtaata ccgcttttaa
    33121 cgctttgatg tattgcggac caaaagccga tgacggaaat attttcagcg cctgcgcgcc
    33181 cgcttcgagc gcggtaaagg cttcggtcgc cgtcgcgcag ccggggcaaa cggtcatgcc
    33241 gtagcccacc gcgcggcgga tcacttcact atggatattg ggcgtaacga tgagctgaca
    33301 gcccatcctg gcgagcgcat cgacctgttc aggtttcagt accgtacctg cgccaatcaa
    33361 cgccttgtcg ccatacgcat caacgatggc gggaatgctc tgctcccatt gtggggaatt
    33421 cagcgggatt tcaaccgcgt cgaacccggc gtcaatcacc gcgccaacat gcgccagcgc
    33481 ctcgtcgggc gtaatgccgc gcaaaatggc gatcagcggg agtttagttt gccactgcat
    33541 gagcgatgct ccttatacca gcctgaaatg ccgtgtcgcc cgccaccgcc gccacgtcgc
    33601 aacccatcgc ctgaaaggct tgctggtagc gcgcggtcag cgatgttccg gcgacaaggg
    33661 tgatggcgtg ttgatgggtc acatagtcgc gcatactggc aacctctgcg ccaatcaaca
    33721 aaccagagag aaattcgctg acctgttcgc ggggaagtgt tcccagcaca tgcgaggcgc
    33781 gaacttcaaa aagctgcggc aatatggcgg gcgcattaag gccgcgctca aggccagccg
    33841 cgaaggcatc ggcagagttt tcctgcggcg gcaaacctgc gccaatcaat gagtgattta
    33901 acagtaaatg atgtaattca ccggtcatca cggtgcgaaa atcgttgatt tgctggctat
    33961 cggcctgcac ccatttgcaa tgcgtaccgg gcataacata aagagaggaa ggagccagag
    34021 ctcgcgcgcc gatcaattgt gtttcttcgc cgcgcatcac attgtggtta tcgtcatgag
    34081 agacgcataa tccgggaata atccagatat tgtcgccaac agacgttaat tgttcgccaa
    34141 tagacgaaaa acgggcagga acagataaat acggagcaac tttccagccg acattgctgc
    34201 cgaccattcc tgccatcacc actggcgtat tttcttcacg ccagtcggtc gtgacttctg
    34261 ctaacaccac agccggagat tttccgttca ggcgcgtgac gcctgcttct gattgcctgc
    34321 tctccaggca gtggtcgccc tgataaagcc aggcgcgcag attggtcgat ccccagtcaa
    34381 ttgcgatgta gcgagctgtc atgtgatttc ctttaacctt cgtgtcgagc tggcaatcat
    34441 ggtaagcgcc gcctgctctg ccgcatcgcc gtcctgatgc cgtatcgcat cgaacagcgc
    34501 cttatgttcc tggagcgttt gcggcatgtt ggcctcatcg cccatccagg ttcgttcaaa
    34561 aaccgcccgc tgcagcgaac tgatcgcaat gctaagttgc tgtaacaccg ggttatgcac
    34621 cgactgcagc accgcctcgt ggtagcgaat atccgcttcg ttaaacgctt cgcggtcctg
    34681 attgttggca atcatctcgt tcagcgccga ttcaatctgc gccagatcgc tggaggtcgc
    34741 gcgctctgct gcccaacggg caatcgccgg ttccaccaga tttcgcactt cgctcatggc
    34801 actgataagc cgtgggtcgt agtcgttttc cagcacccat tgcagtacgt cagtgtcgag
    34861 gtaattccac tggttacgcg gtgccacaaa cgccccgcga taacgtttca tttcaatcag
    34921 ccgcttcgcc atcagcgaac ggaacacctc acggatgatg ttgcgcgagg ttgcaaactc
    34981 ttcacagagc tccgcctcag ccggaagtgg cgagccaggt acgtatttgc cgtgaacgat
    35041 ctgtttaccc agcgtaatga caatgcgatc ggttttattg agagtcatgg agagtccttg
    35101 tgctctgtat gttcttctct actttacccc gatcgatgca taacgcggca actttgtagt
    35161 accagcgtga tgacgttcgc gtttgccgcg cgcgtaatgt agtacaaact tatattgtta
    35221 tgctacaatt tagatcaaaa aaagaacaat acataaaaaa atgacatgcg tcgggcagaa
    35281 atctgaaaag ggatatcagg cgctaaacag gagggaaaga agagtatgct ttcaacggct
    35341 tagctactcg tttaaaggat taatcatgaa gttgaatttt aagggatttt ttaaggctgc
    35401 cggtttattc ccactggcgc tgatgctttc aggctgtatc tcgtatgctc tggtttccca
    35461 taccgcaaag ggtagttcag gaaagtatca atcgcagtca gacaccatca ctgggctatc
    35521 gcaggcaaaa gatagtaatg gaacaaaagg ctatgttttt gtaggggaat cgctggatta
    35581 ccttatcact gatggtgccg atgacatcgt taagatgctc aatgatccag cacttaaccg
    35641 gcacaatatt caggttgccg atgacgcaag atttgtttta aatgcgggga aaaagaaatt
    35701 taccggcaca atatcgcttt actactactg gaataacgaa gaagaaaagg cactggcaac
    35761 gcattatggt tttgcctgtg gtgttcaaca ctgtaccagg tcactggaaa acctaaaagg
    35821 cacaatccat gagaaaaata aaaacatgga ttactcaaag gtgatggcgt tctaccatcc
    35881 atttaaagtg cgattttatg aatactattc acccagaggc attccggatg gtgtttccgc
    35941 agcattactg ccagtgactg ttacactgga catcattact gcaccgctgc aatttctggt
    36001 tgtatatgca gtaaaccaat aatcagtaag cgggcaaacg cgtttatgct gtttgcccgc
    36061 ttacagatta attcagcaca tacttctcaa tagcaaacgc cacgccatct tcaaggttgg
    36121 attttgtgac aaagttcgcc acttctttca ccgaaggaat agcgttatcc atcgccacac
    36181 cgacgcctgc atattcaatc attgcgatgt cgttttcctg atcgccaatc gccatgattt
    36241 cttccggttt aatacctaac acgtcggcca gtgatttcac acccgtacct ttgttaacgc
    36301 gtttatcgag gatttcgagg aagtacggcg cacttttcag cacggtatat ttctctttca
    36361 cttcctgcgg aatacgcgcg atagcctggt cgaggatggc gggttcatca atcatcatca
    36421 ctttcaggaa ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaattg
    36481 tggcaacgaa ggattcatgc accgtgtagt agctgatatc gcggttagcg gtgtacagcg
    36541 tggtgcggtc cagggcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac
    36601 gatagtcgtc atagctgaga gcggtttgcg ccacggtgct accatcagcg gccttctgta
    36661 ccagcgcgcc gttataagta atgcagtagt cgcccggctg ttccatatgc agctctttca
    36721 ggtagttgtg cacacctgca tacggacgac ccgtcgttag cacgacattc acgccacggg
    36781 cgcgagctgc ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa
    36841 gggtgccatc catatcgata gcaatgagtt tgatagccat gagttcccaa ggtagattag
    36901 ttcctgaccc atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg
    36961 ggatgcaacg cgggttgcat gctccccatt tgcttagcgg aagagtttcc ctttcagcag
    37021 ttccatgcct gcggaaagca gatcgttatt ggcttgtgct gacacttcac cttgcggtga
    37081 gagcgcatca ataatcttcg gcaattgctc tgccagtaaa ctggaagccg tactggtatc
    37141 cacgccaagt ttttgcccga gatcggacac cgcatttgtg ccgagcgccg actccagttg
    37201 ctcgccgcta actgattgat ttctctgttg attactcaac caggttgaga gaatggcccc
    37261 taagccgcca ctttgcagtt tttccagcag cacctgaatg ccaccctgct cctcaaccca
    37321 acttaaaatc gcctgatatt tccccgcatc gcctttcaga aaggcaccga caacttcatc
    37381 aaaaagcccc atgataatca cctgtaaagc gttacgtgtt gacccaaaaa gtatagatct
    37441 gcgggatgat aattgcggat tgcagaaata aaaagggcgg agatgatctc cgcccttttc
    37501 ttatagcttc ttgccggatg cggcgtgaac gccttatccg gcctacaaaa tcatgcaaaa
    37561 tcaatatatt gcaggagtct cgtaggacta ataagcgtag cgcatcaggc acgctcgcat
    37621 ggttaacgcc attaaatatc aatattcgcc gctttcaggg cgttctcttc aataaacgca
    37681 cgacgcggtt ctacggcgtc gcccatcagc gtggtgaaca actggtcagc ggcaatcgca
    37741 tccttaacgg taacgcgcag catacgacgg ctttccgggt ccatggtggt ttcccacagc
    37801 tgttccgggt tcatctcgcc cagaccttta taacgctgga tggagaggcc acgacgggac
    37861 tctttcacca gccagtccag cgcctgctcg aagctggcta ccggctgacg acgctcgcca
    37921 cgttcgataa acgcatcttc ttccagcaag ccacgcagtt tctcacccag cgtgcagata
    37981 cgacgatatt cgccaccggt gataaactcg tgatccagcg gatagtcagt atccacaccg
    38041 tgggtacgca cgcgaacaat cggctcgaac aggttttgtt cggcattggt gtggacatca
    38101 aacttccact ggctgccgtg ctgttctttg tcgttcagtt cgctgaccag cgcgttcacc
    38161 cagcgggtaa cggtctgctc atcagaaagg tcagcttccg tcaacgtcgg ctgatagata
    38221 agctctttca gcattgcttt cggataacga cgctccatgc ggttgatcat tttctgcgtc
    38281 gcgttgtact cagataccag tttctctaac gcttcgccag ccaatgccgg tgcactggcg
    38341 ttggtgtgca gcgttgcgcc gtccagcgcg atagagatct ggtactgatc catcgcttcg
    38401 tcgtctttaa tgtactgttc ctgcttgcct ttcttcactt tgtacagcgg cggctgagcg
    38461 atgtagacgt gaccgcgttc aacgatttcc ggcatctgac gatagaagaa ggtcaacagc
    38521 agcgtacgaa tgtgcgagcc gtcgacgtcc gcatcggtca tgatgatgat gctgtgataa
    38581 cgcagtttgt ccgggttgta ctcgtcacga ccgataccac agccaagcgc ggtgataagc
    38641 gtcgccactt cctgagaaga gagcatctta tcgaagcgcg ctttctcgac gttaaggatt
    38701 ttacccttca gcggcagaat cgcctggttc ttgcggttac gcccctgctt cgcagagccg
    38761 cccgcggagt ccccttccac caggtacagt tcggaaagcg ccggatcgcg ttcctggcag
    38821 tctgccagtt tgcccggcag gccagctaag tcgagcgcac ctttacggcg ggtcatttca
    38881 cgcgcgcgac gcgccgcttc acgggcacgg gcagcatcga taattttgcc aaccacgatt
    38941 ttcgcatcag ttgggttttc cagcaggtat tccgccagca gttcgttcat ctgctgttca
    39001 accgccgatt tcacctcaga agaaaccagt ttgtctttgg tctgggagga gaatttcggg
    39061 tccggcactt ttacggaaac gaccgcaatc aggccttcac gcgcatcgtc accggtggcg
    39121 ctgactttgg cttttttgct gtagccttct ttgtccatgt aggcattcag ggtacgggtc
    39181 atcgccgcac ggaagcctgc caggtgagta ccgccgtcac gctgcggaat gttgttggta
    39241 aagcagtaga tgttttcctg gaagccatcg ttccactgca gcgccacttc aacgccaata
    39301 ccgtcttttt cggtggagaa gtagaagata tttgggtgga tcggcgtttt gttcttgttc
    39361 agatattcaa cgaacgcttt gatgccgcct tcatagtgga agtggtcttc tttgccgtca
    39421 cgcttgtcgc gcagacgaat ggaaacgccg gagttgagga acgacaactc acgcagacgt
    39481 ttcgccagaa tttcatattc gaactcggtc acattggtga aggtttcgag gctgggccag
    39541 aaacgcacca tggtgccggt tttttcagtc tcgccggtaa ccgccagcgg agcctgcggt
    39601 acaccgtgtt cgtagatctg acggtgaatt ttaccctcgc gctgaataac cagctccagt
    39661 ttttgcgaca gggcgtttac taccgaaaca ccaacgccgt gcagaccgcc ggacacttta
    39721 taggagttat cgtcaaattt accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc
    39781 gatacgccct cttccgggtg aataccggtc ggaatgccgc gcccgtcatc ctgtacagag
    39841 acagagttat cggcgtgaat ggtgacgata atttctttac agtgacccgc gagcgcttcg
    39901 tcgatagcgt tatctaccac ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg
    39961 tcgccgatat acatacccgg gcgcttacgc accgcatcca gccctttcag gactttgata
    40021 ctggaggagt cataagaatt cgacatcaac gtttctcgct catttatact tgggttaatc
    40081 tgttatttta cccttttcca cggtaaacat cttcgaattt tcgtccgaca tgtctataac
    40141 gtgttcagca ctgatcgcgc tgacaaagac ctgtgattgc gtcgctttta agcggctggc
    40201 aagcaacccg cgacgctcat catcaagctc agaggcaaaa tcatctatca ggtagagaca
    40261 ccgccgcccg ctttcacgag tgaggaactc tccttgcgcc agacgtaagg cgcacatcaa
    40321 cagcttaagc tgcccacgcg ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg
    40381 taagtccgct ttatgcgggc catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt
    40441 acgttccagc acctcagcgt actctgtctc tttctcccag ccgcgctgga aagagaaagt
    40501 cagagaaaac tcagggagaa attgcttaca ggtatcatcc atatcggccg cgataccggc
    40561 gctatactcc gcgcgccagg tgctgatttg ctccgccagc gggatcagtt ctttatccca
    40621 cgggcgtagc tgttcgtaac gagtcacctg gcgcagcgcc gcattgcgct gcttgagcaa
    40681 tcgcttgaga ttgctccagg cggtgaaaaa tccaggttcg ttgtgaaagc atccccagtc
    40741 gaggaatgct cttctgtatt tggggccgcc gttgagtaaa gtaaaccctt ctggcgttat
    40801 caactgcatc ggcatcaggt gcgccagttc cgcgacttta tgcccgtccg taccgtcaat
    40861 acggactttg ctgtcgccct gtttgtcttt ggttaagcca atcgccgtct cgcgctcttc
    40921 gccctgtaat cgcccgtgga gaacaaatgc ctcctgctca tggcgaatga cgcgaccaat
    40981 ctgcaaactg cgaaacgccc gaccatggcc gagcgtatag atggcttcca gcacgctggt
    41041 tttgccactg ccgttggcac ctaccagaaa gttaaagccg ggagataaag cgagatccgc
    41101 ggtttcaata ttgcggaaat cgcggatcaa caagcgggtg agggacatta cagtctcatt
    41161 ggcatgacaa cataggcagc cgactgtgat gcggcatctt caatctgcac gctggaaacc
    41221 gaatcggtca gcatcatgcg gacgttttcg catttcagcg cgttcagaac atccagcaca
    41281 tagctgacgt tgaagccgat ttccatctcc gcaccgctat aggtaacgtc gaggatctct
    41341 tccgcttctt cctgttccgg gttgttggca gtaattttca gctggttttc gctgacatac
    41401 aggcgcacac cgcggaattt ctcgttagag agaatcgccg cgcgagcaaa cgcctgcttg
    41461 agcagatcgc agcctgcttc cagatgtttg tccgggttct tcggcagaac gcgacggtaa
    41521 tccgggaagc gaccatccac cagtttcgag gtgaaaataa agtcgccaac gtgggcacga
    41581 atgttattgc tgccaatctg cacacgcagc agattgtcgc cgccgtcgag catacgcatc
    41641 agttcaatca cgcctttacg cggtacgatc accgaatggc ttggcaaaga ttgaccaatt
    41701 ggcattgaac agaccgccag acggtggccg tcggttgcca cggtgcgcag ttcttcacct
    41761 tcggtttcaa acagcatacc gtttaagtaa tagcgaacat cctgatgcgc catcgaaaac
    41821 tgggttgctt caatcagacg cttcatcgtc gcctgcggca gggtaaattc gacttcgctc
    41881 tgccagtcat cgaggttcgg gaaatccgcc gctggcaggg tagacagcga aaaacggcta
    41941 cgcccggagc gcaccagcat ccgttcacct tccagctgca cggcaatttc cgcgccttca
    42001 ggcagaccac ggcagatatc aaagaatttg cgcgccggaa cggtcgtcgc tcctggctcg
    42061 tgtggctgaa ccagcgcaac gcgtgccacc atttccatct cgagatcagt accggtcagc
    42121 gacaacgtac cgtcagcaac ctgtaacagc agattaccga gaatcggtag cgtaggacga
    42181 ccacctaacg gaccgctcac ctgttgtagc ggttttaata aatgctcacg ttctacggta
    42241 aatttcatag gtttacgatg acaatgttct gattaaattt gaaaaatctt ctttgatatc
    42301 gtggctctct tcacgcaact gctcgatctt acggcaggca tgaagcaccg tcgtgtggtc
    42361 acgcccacca aacgcatcgc caatctctgg cagactgtgg ttagtcagct ctttcgctag
    42421 cgccatcgcc atctggcgcg gacgcgccac cgagcgggat cgacgcttgg aaaggagatc
    42481 cgcgactttg atcttgtagt actccgccac cgtcttctga atattgtcga tggtgaccag
    42541 tttttcctgc aatgccagca agtcgcgcag cgcctcacgc acgaagtcga tggtgatcgc
    42601 ccgtccggta aagttggcgt tagcaataac gcggttcagc gccccttcca gctcacgtac
    42661 gttagatcgt agacgcttgg cgataaagaa cgccacttca cccggcaaac gaatgtcatt
    42721 ttcgtcggcc tttttcatca ggatcgccac acgggtttcc agctctggcg gttcgatcgc
    42781 cacagtcagt ccccaaccga agcgggattt caaacgatcc tcaacgccgt tgatctcttt
    42841 cggatagcga tccgaggtga gaatgatctg ttgattacct tccagcaggg cgttgaaggt
    42901 gtggaaaaac tcttcctgag atcgttcttt attagcaaaa aactgaatat cgtcgatcag
    42961 cagtgcatct acggaacggt agtagcgttt aaactcttcg atcgcgttgt tttgcagggc
    43021 tttaaccatg tcctgaacaa agcgctcgga gtgcatataa accactttgg cattcggctt
    43081 gcgcgccata atgccgttac ccaccgcatg cagcaggtga gttttaccca gacccgtgcc
    43141 gccataaagg aacaacgggt tataggcacc gccagggtta tccgccacct ggcaagccgc
    43201 cgcgcgcgcc agttggttag atttaccttc aacgaagtta tcaaacgtgt gtttgacgtt
    43261 tacgttagaa cgataggtcg gttctgccgg ggccgggacg ttatcccaac ctgagcgcgt
    43321 agaaggcgca gcacgttgcg gctgcgtttg cgccacctgt gcaggggccg cgacgttgct
    43381 cgtcactgcc gcttgtggcg tttgcgtcac cggtttggtg ccgacttcaa aacgcagctg
    43441 tggggcatcc gctccgcaga aactggttag cagtccattg atattattaa ggtacttgtc
    43501 ccgtacccaa tcgaggacaa aacggtttgg cgcgtacagg gccagcgtgt tatcgctcag
    43561 ttccgcctgc aatgggcgta tccacatact gaattctgtg gctggtaact catcctgcaa
    43621 tcgggcaaga cactgctgcc aaagcgaaag tgacacggcg gactccactc gaacaaaagt
    43681 cgataatgac taaggctgaa acattcatga ttgttgacgt acgtcgaaaa gaccctgctt
    43741 gagggtgacg cacgaaccgc tgtctgcggt tatatgcccg atcaagatcc tgcaaaacga
    43801 tcgggaccgc ggatcatagc ctaaactgcg caagagatct tctgtttctc acagattttc
    43861 cctatttatc cacaggactt tccagaactc gctaagtgta aacgatcctg ccgcgaggcg
    43921 ggcacgattt acgccgcata ttggaaaatt taatgaccat agacaaaaat tggcttaatc
    43981 gatctaataa agatccagga cgatccttgc gctttaccca tcagcccgta taatcctcca
    44041 cccggcgcgc catgctggtt tccactggtg tgaggtcgta cattttccct gcgaaaaggt
    44101 gcggaaaagc gcggtaaata aggaaagaga attgactccg gtgtgtacaa ttattacaat
    44161 ccggcctctt taatcaccca tggcttcggt gtccatcgtt tcatttttcg gcggatatcc
    44221 aataaagcca ttgaatttat tcaagtttag gtagaaatcg ccatgaaacg cacttttcaa
    44281 ccgtctgtac tgaagcgcaa ccgttctcac ggcttccgtg ctcgtatggc tactaaaaat
    44341 ggtcgtcagg ttctggcacg tcgtcgtgct aaaggccgcg ctcgtctgac cgtttctaag
    44401 taataaagct aacccctgag tggttaagct cgcatttccc agggagttac gcttgttaac
    44461 tcccagtcaa ttcacattcg tcttccagca gccacaacgg gctggcacgc cgcaaattac
    44521 cattctcggc cgcctgaatt cgctggggca tccccgtatc ggtcttacag tcgccaagaa
    44581 aaacgttcga cgcgcccatg aacgcaatcg gattaaacgc ctgacgcgtg aaagcttccg
    44641 tctgcgccaa catgaactcc cggctatgga tttcgtggtg gtggcgaaaa aaggggttgc
    44701 cgacctcgat aaccgtgctc tctcggaagc gttggaaaaa ttatggcgcc gccactgtcg
    44761 cctggctcgc gggtcctgat agccctcatt cgggtctatc aacgcctgat tagtccgcta
    44821 ctcgggccgc attgtcgttt cactccaacc tgttcaagct acggaattga ggcattgcgc
    44881 aggtttggag tgataaaagg cagttggttg acggtgaaac gcgtattaaa atgccaccct
    44941 ttacaccctg gtggtgacga tcccgtcccg cccggaccat ttgataccag agaacactaa
    45001 cgatggattc gcaacgcaat cttttagtca tcgctttgct gttcgtgtct ttcatgatct
    45061 ggcaagcctg ggagcaggat aaaaacccgc aacctcaggc ccaacagacc acgcagacaa
    45121 cgaccaccgc agcgggtagc gccgccgacc agggcgtacc ggccagtggc caggggaaac
    45181 tgatctcggt taagaccgac gtgcttgatc tgaccatcaa cacccgtggt ggtgatgttg
    45241 agcaagctct gctgcctgct tacccgaaag agctgaactc tacccagccg ttccagctgt
    45301 tggaaacttc accgcagttt atttatcagg cacagagcgg tctgaccggt cgtgatggcc
    45361 cggataaccc ggctaacggc ccgcgtccgc tgtataacgt tgaaaaagac gcttatgtgc
    45421 tggctgaagg tcaaaacgaa ctgcaggtgc cgatgacgta taccgacgcg gcaggcaaca
    45481 cgtttaccaa aacgtttgtc ctgaaacgtg gtgattacgc tgtcaacgtc aactacaacg
    45541 tgcagaacgc tggcgagaaa ccgctggaaa tctcctcgtt tggtcagttg aagcaatcca
    45601 tcactctgcc accgcatctc gataccggaa gcagcaactt cgcactgcac accttccgtg
    45661 gcgcggcgta ctccacgcct gacgagaagt atgagaaata caagttcgat accattgccg
    45721 ataacgaaaa cctgaacatc tcttcgaaag gtggttgggt ggcgatgctg caacagtatt
    45781 tcgcgacggc gtggatcccg cataacgacg gtaccaacaa cttctatacc gctaatctgg
    45841 gtaacggcat cgtcgctatc ggctataaat ctcagccggt actggttcag cctggtcaga
    45901 ctggcgcgat gaacagcacc ctgtgggttg gcccggaaat ccaggacaaa atggcagctg
    45961 ttgctccgca cctggatctg accgttgatt acggttggtt gtggttcatc tctcagccgc
    46021 tgttcaaact gctgaaatgg atccatagct ttgtgggtaa ctggggcttc tccattatca
    46081 tcatcacctt tatcgttcgt ggcatcatgt acccgctgac caaagcgcag tacacctcca
    46141 tggcgaagat gcgtatgctg cagccgaaga ttcaggcaat gcgtgagcgt ctgggcgatg
    46201 acaaacagcg tatcagtcag gaaatgatgg cgctgtacaa agctgagaag gttaacccgc
    46261 tgggcggctg cttcccgctg ctgatccaaa tgccaatctt cctggcgttg tactacatgc
    46321 tgatgggttc cgttgaactg cgtcaggcac cgtttgcact gtggatccac gacctgtcgg
    46381 cacaggaccc gtactacatc ctgccgatcc tgatgggcgt aacgatgttc ttcattcaga
    46441 agatgtcgcc gaccactgtg accgacccga tgcagcagaa gatcatgacc tttatgccgg
    46501 tcatcttcac cgtgttcttc ctgtggttcc cgtcaggtct ggtactgtac tatatcgtca
    46561 gcaacctggt aaccattatt cagcagcagc tgatttaccg tggtctggaa aaacgtggcc
    46621 tgcatagccg cgagaagaaa aaatcctgat tcggtgagtt ttcgctaaaa taagggcggt
    46681 cagttgaccg ccttttttct tttcgtaggg cggataagca ccgcgcatcc gccacacaaa
    46741 gcaacaggaa catcatgagc gataatgaca ctatcgtagc ccaggccacg cctccgggac
    46801 gtggcggcgt tggcatcctg cgcatctccg gcttcaaagc ccgtgaagtt gccgaaaccg
    46861 tgctgggtaa actgcctaag ccgcgctacg ccgattatct tccgtttaaa gacgccgacg
    46921 gcagcgtgct cgatcagggg attgcgctat ggttccctgg cccgaactcg ttcaccggcg
    46981 aagatgtgct ggaactgcaa ggtcatggcg gtccggtgat cctcgacctg ctgttaaaac
    47041 gcattctgac cattcccggc ctgcggattg ctcgccctgg tgagttttcc gaacgcgcgt
    47101 ttcttaacga taaacttgac ttagcccagg ccgaggcgat tgccgatctt atcgacgcca
    47161 gttcggaaca ggcggcccgt tcggcactta actcgctgca aggcgcattc tccgcacggg
    47221 ttaatcatct ggtagaagcc ctcacccact tgcgcattta cgtcgaagcg gcaattgatt
    47281 tccctgatga agagatcgat ttcctctccg acggcaaaat tgaagcccag cttaatgacg
    47341 tgatggccga tctcgatgca gtgcgtgctg aagcacgtca gggtagtttg ttgcgcgaag
    47401 ggatgaaagt agtgattgcc ggacgtccta atgccggtaa atcgagcctg ttaaacgcgc
    47461 tggcgggccg tgaagcggca atcgtaaccg atatcgccgg aaccacgcgt gacgtgctgc
    47521 gtgagcatat ccacattgac ggaatgccgc tgcatatcat cgacactgcc gggctacgtg
    47581 aggccagtga cgaagtggaa cgtattggta tcgagcgcgc gtggcaggaa attgaacagg
    47641 ccgaccgcgt gctgtttatg gtcgatggca ccacgacaga cgccgttgat ccggcagaga
    47701 tctggccgga atttatcgcc cgtctgcccg cgaaactgcc gatcaccgtg gtacgcaata
    47761 aagccgatat caccggcgaa acgctgggaa tgagtgaagt gaacggtcac gcgttaattc
    47821 gtctctcggc aaggactggt gaaggcgtgg acgtgctgcg taaccatctc aaacagagca
    47881 tgggctttga caccaatatg gaaggcggct tcctggcgcg ccgtcgccac ttacaggcgc
    47941 tggaacaggc agcggagcat ttgcagcaag gtaaagcgca actgttggga gcctgggcag
    48001 gtgaactgct ggcggaagag ttgcgcctgg cacagcagaa cttaagcgaa atcaccgggg
    48061 aatttacttc agacgacctg ctggggcgga ttttctccag cttctgtatt ggtaagtaac
    48121 cgcgcttacg aagccgtatt ctgactgtca gatgcggctt cgcttcattg ttagcactcc
    48181 tgttatgcct caaccctttt ttaaacatta aaattcttac gtaatttata atctttaaaa
    48241 aaagcattta atactgctcc ccgaacgatt gtgattcgat tcacatttaa acaatttcag
    48301 aatagacaaa a
//
LOCUS       DAJVRP010000021        48096 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVRP010000021 DAJVRP010000000
VERSION     DAJVRP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.021"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..560)
                     /locus_tag="OQN12_001178"
     CDS             complement(<1..560)
                     /locus_tag="OQN12_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1653587.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALESAGCELIFEDKASGKKAERPG
                     LKKVLRMLSRGDTLVVWKLDRLGRSMRHLVVLVEELRDIGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLDAARAEGRIGGRRPKYQEETWQQMRRLLEKGIP
                     RKQVAIIYDVAVSTLYKKFPASSFQS"
     gene            666..992
                     /locus_tag="OQN12_001179"
     CDS             666..992
                     /locus_tag="OQN12_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCS1653588.1"
                     /translation="MFADYENLAVVVITSLLSGTGVFLLGVRDGRISASLLNLASELF
                     TAVTAGLAGYGVAVSQEWPEGIIFCVVLIASNNGREILQGLKSRASNVLNLLSVIANG
                     GKGGEK"
     gene            992..1438
                     /locus_tag="OQN12_001180"
     CDS             992..1438
                     /locus_tag="OQN12_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1653589.1"
                     /translation="MGNFGNYFIFIMAFVAIIDRFIFRRKSVEILSIGDAVVKESAIA
                     FPVSLRVKRSWVSNAQIEYWLQDLKNPSVVISGKTRPVDASKRGEREEYLLIDTKYLE
                     PTKWDLKVTLTNGNCRLNPLYRIFPINDCIERQFTIEKREGDWDVK"
     gene            1428..2048
                     /locus_tag="OQN12_001181"
     CDS             1428..2048
                     /locus_tag="OQN12_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653590.1"
                     /translation="MSNKNNYVLLNYDELNEKGLAKLVKEISKGGYKIARVVPASNGR
                     KKDGIMTRTFTLIGLDEQTMEVQVNDTGDISGIKLNGKTVPFKPVKTMSALGQSLAAL
                     FNRGATSFQKSLARKLARAAKNIDDENKKRQGVKSNAQRLAEAKESRDALREDIANAN
                     DKLSKLQSKSDKVMQDTGNVKAALSQEVAKTRALKEEIARLEDEHD"
     gene            2041..3966
                     /locus_tag="OQN12_001182"
     CDS             2041..3966
                     /locus_tag="OQN12_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head protein"
                     /protein_id="HCS1653591.1"
                     /translation="MINNPLRINIQSTFADTIPGYMPGRWSSEELSEDDLIFEAATLE
                     DIELAYMGNEILVVSDEAMFESITTTRIRLAQTMRAFVRALNRGLNGTNIMAGTDEAG
                     TDENGRNCIGGAIIGRVRRVANIPVLTAQIPLTDGQSTSIIFHSPTADGAKIKSNDEL
                     VAFQFLLNKRNVTHVVAPIGGRDVSLSQVCQALSNLIERNSEKFKKAKERQDKMKADI
                     ESYLNEADKLAEERSITIDQVDVAQRGLTEKRAALTEMQKKLDAQKAINEELQEKRDK
                     LLSAKGEKTKERAFSDQLRHVKHNLAIDGKTVLDNGAEVNYVTSGSDNFVTIVAPEGK
                     FSIDASAVKGGSLADAATKLLKAYREHNADKYKVDVLPGQQEDSEPPQTAPETNPVSQ
                     PESQDVGKYHYALQSRPAGVGAVPDGNKAVLDRPDQADQYYGYARHGIITYDRKLTDK
                     EVSQYELLYLPDEDELKDFAGQLVVSSMSKHIDGYVDLFGSDLKTFKAQVKILFRKAF
                     PNVAYPLGDGENLFIMDVYNALQNYSSEVKTEHVVEPESQPQPAPEQEPEPEEASESA
                     SEADQEADKALEYLKSVPEQFTSRDLTVISTELDHVQEAANALISAGRYDENEATVGA
                     AVDYLINILAEIQQGGA"
     gene            3966..4334
                     /locus_tag="OQN12_001183"
     CDS             3966..4334
                     /locus_tag="OQN12_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653592.1"
                     /translation="MTISVLDRLKLGKELSDLMQAQKTAPVLQRVTIGKQIVDVMLKL
                     GLGTAAQPAAEPQQQTNPVVDEVPKIVTDFLGGVFTKSTQMEFIDALRGISNYVGEFL
                     TLEQAKEQTISWVKANGYAG"
     gene            4429..5814
                     /locus_tag="OQN12_001184"
     CDS             4429..5814
                     /locus_tag="OQN12_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653593.1"
                     /translation="MNKSVTSALSEAADINSVIALVSSLERKETRMGRSSYVVTSKGA
                     EVKTAFKVVDASSLIISNNLDGTINPAFPEELQPRDRTRLSSKLQVNRIASDLRPAQL
                     TDSGMSSHGAPIVGPDNVVESGNGRSMGIWRAYEQGQADDYRQYLIDHAKEFGLNPDE
                     ISQMSMPVLVRERLTDVDRAQFARDSNISDLQEMAASEKAYADAQFLTESVMALFNPS
                     DDGNLLARSNDAFIRAFLREIGDTATAGLLTADGRPTKQLIDRIQNAIFAKAYKDERL
                     VRLVSEEPDPEMRNILTALNTAASDFAQMQSLSGDAHHDAVTGLVDGIEQVNGLDTQA
                     IAALQEAINLVREAKDNGQAVEEVIAQRGLFGDSTPEAEALALFIVANNRSAKRMGAA
                     FKKMAQKINDELTHKQQALGDMFGGGDVDLRSILSAVSDEIEGEFGEGKGLNFAMFEN
                     STVPNNCRTEN"
     gene            5879..7561
                     /locus_tag="OQN12_001185"
     CDS             5879..7561
                     /locus_tag="OQN12_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1653594.1"
                     /translation="MATKDKNKGFLSALKKAFNGGDVTPADPVVFTSGHSVVARSGLS
                     ALRPGILGSNSDGMTSEADSISLSAELPGERLQKYNILETMAKSPTISTALNIHIAHA
                     LAPSKKNGQAFILSPKDGSDAEAVSRCEELTADLGAMINDGLPSWAMIMAIFGVSYVR
                     PYAEQGRGITGIESSYYTLPHFVQEFYRGSQLVGFSGDYILDTHSLRRVITEPWNLVS
                     MKNPYWTPQHKVIPVSYGTKGYSLLSDQADKPLMETQNYGTSFLEYSYEPYLNLCASL
                     AALKSTRNNAAKIDRLIALTTNTLDPVNAANYTRGVSQALKRHSDLVAQRSINANAIP
                     TVLNHLIPVMGDGKNGITIDTQSIPADISGIEDVMFHLRQLAASLGIDATMLGWADQM
                     SGGLGEGGWQQTAIQAALRANWIRQAAQRTIYRLLDIHLAYKYGKVYTEKNRPYDVQF
                     NSMNTAIQEEENRELDARANFVAVISQIMDQIQNNPKLAGSDAFMRYLFTEQLHIDDD
                     TLNTMIKEFKANESEQNNEHGMYESAPLSSGDDPENWTPEQLINFAKFVMSN"
     gene            7575..8573
                     /locus_tag="OQN12_001186"
     CDS             7575..8573
                     /locus_tag="OQN12_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HCS1653595.1"
                     /translation="MQSLNTVTDRFSLVEKIRKHTPQNNRNYVIQSVRDTFNSPETKE
                     RIALGEMYGYYGHGRRAMHYNKTKSLNLPEVSVVMVDGKPVVLENVPSNRTIDISIDD
                     NGIVTHTQEILDTDTGRIVQGMINSGAGGWSWATSGPDSSVSLVKSFHGFDYVTVPNY
                     ISLDKKSLMLESAEERDAAIHAALIEQGFTDNAATDLVHHFSAMKNQQAIFESAQREA
                     LESELMLLEVENIQLRDKLRTQTAMMESQGENAKQCRRILRDAIQEMPVFISAEQRRA
                     LCRMQSEDDARIVAAMLESLGANATYGLPILGKKEPELSPKESKNTTPLLFVSRRA"
     gene            8774..9736
                     /locus_tag="OQN12_001187"
     CDS             8774..9736
                     /locus_tag="OQN12_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic replication protein"
                     /protein_id="HCS1653596.1"
                     /translation="MNILNSVSVTFNTSEYKNNTEYHKKIKGHQLSFFARHSGLLSPS
                     HSAIIAEFANLAGATDEYMIRRSYADMSEITGRSISTVRRAFAEATKCGMLVRQHQVA
                     NNNAQVCNVYRFTTQFLHFIHAAMEIGGKQGIKFANATKLVKQLISKVRYFFETGNPL
                     FILNKSPHVQNEQPIENKSHSIAKRRERSCAVQPKASPADSSQADNGVLDKKPVIREE
                     HTNRCLAAAKARAAKRRSDEGHAKRQALYRTAEKLAKKFAWIRSAATAVNKPKQSSAL
                     DFSMDYSGSQGCATIGEAFDLMKQRGYRSEFDREDWSIPAGFRG"
     gene            10150..10374
                     /locus_tag="OQN12_001188"
     CDS             10150..10374
                     /locus_tag="OQN12_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1653597.1"
                     /translation="MVNANPCSRPEFIWRFHSLQKHCDHFAIAATEKEARSLIPDQYG
                     VLTGRFSTSPQPNTNPWYRNITNPSIAEAH"
     gene            complement(10477..10962)
                     /locus_tag="OQN12_001189"
     CDS             complement(10477..10962)
                     /locus_tag="OQN12_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HCS1653598.1"
                     /translation="MANIEPRWLIEARKHIGLTEIKGAKHNPEIVQFWRDIKRGGIKD
                     DETPWCAAFVGAMLERVGIRSTRFESAKSYLDWGAKLDTPAYGCIVVFTRVGGGHVGF
                     VVGRRANGDLLVLGGNQGDAVNIRAFPTSRVSGYRWPAGEPRNTALLPVGDAATSTNE
                     A"
     gene            11113..17955
                     /locus_tag="OQN12_001190"
     CDS             11113..17955
                     /locus_tag="OQN12_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCS1653599.1"
                     /translation="MDKLLHSISEASSLGELIEIVSRLHKLRAVATFGVKKVDGVSIQ
                     KTRRAANNAAVELLNSLPPGFDGAKLTDEQRQILAGYTGEGGLTDGEGSQYEYYTPQF
                     MAEGIWDLFADYGIDGGHVLEPSAGTGIFQETKRQGMIMTSAELSPISGRINQLLHPE
                     DDVNIGAFEALAAKDAMYDHAVGNVPFGEGRSGVAGLDPAYANEKNVGNYFVLRTIDK
                     VKPGGLIVLVVPNGMTDGTKYKKLRDKVSRKAEFLGAHRMPSGTFSESGTDTVVDVWV
                     LRKHPETFLEMIPDTDDSTLKSANVLWDTFLKGKWFTTEGKRFVYGDMERTSFRNTLV
                     VKKDGRVSNESMKTALSRRFDSRINWDLLGVTTQAWQGAKVGDKRLVGGIWHEFDGLK
                     WVKDTTTKSSALDVSRYGVSTFGDLQTTFQSTNGILALSWGQISAIASDYPSVISDEV
                     AAMIRFAGKQREKDRERIMRGALIGQLINKALDKRNLGENVDDELADAARLTAAEVAK
                     YGPPHAIKLNDITEAGAKNWLTFTGNVNQDGEASDLLSGRLDVSKGAAGIDFTRPEQV
                     VTHLFSDVALNPINLDDFREAFKGELPEDDDQALEYLAKFDDIAIDGYGCLLPMGRAT
                     SGDIKSKVALLVGWRDASTGERKANFERQLSKIEEKRTFTPLNKVTVNLNARWLDRRL
                     IKEFLAEQGYDEFKYTEPDLKVEDGILVSPDDYEGKDGVFTGYQLRTVNGKNGNEFKK
                     ANNKDGFLNQLENYLNGVKPRGQNANEYLDKIAQLETSFNDWLRTHPQADQIARDYND
                     AFNGYIPFTHSDAPLGLEGISGKRIPLSYQNEEVRRLSEDGRGIMGFGTGLGKTTTAL
                     ALEAYNFETGRTKRTCIVVPKAVYQNWYHEAQSFYSADAFANMMFIGLDEVRDGDGNI
                     QTAPVLDQDGTPRLDNDGNPITRNVVKESASAVIAQRMNMIPSSNWRTVIMTKEQFAS
                     IPLRDETIGENSQQAVFNAAEMGRLDLASGKHRDAQKKNKIKDQAANTGTAKKQNIPY
                     FEDMNFDSVIADEGHNYRNSFGAGREAGQLAYLPNPAVSKMARDMAVKAAYMMKRHNG
                     RGVVMLTATPLVNSPIDAFNMLSTVIPQHEWMHMGIITPDDFVRVFGKTATVQVQKIS
                     GEVEEKQGLVGFQNLDGLRGIFHRWTTLKTASDVGATVKIPDIVENTLQIPMTPEQQA
                     AYEELRKRAQELSRGDDVTVDDEGNITGGKPDDFIFSIIRDMDKVAIDPDLYASAITF
                     QFPVELTEQVKSIANNLPKVAGGKAADDAEEAEEDATGGLVSTRTSKVVATTFSEHPN
                     HVELRASIDLEAEILKAIADAGIDMKQVSHPIPPKYAALIENLREGLKNGKQIIFIDE
                     KAQHQKLRRIIASALQMPEQEIGIINATTVSQAGGVKLKKVKKPTEPTPNKDGEYKEG
                     AWETYYSKLAQYEDYLSAKNDAGLEGMEGIAADYNEGRTRIIICNKKAEVGINLHIGT
                     TDIHHLTLPWTPASIDQRNGRGARVGSPQEKVNVHYYCGKGTFDDFRLDTLKRKKDWI
                     KMVMTSDMSEIANGDADDADERAIMLAANPEERRAIMARQSKEREERLRLKAQREANN
                     ALDNYLKAANAAGKDIGMMEAELKSAMEQAQRYQSDLDGLIKSGTNKNGQKYTLSALS
                     KAKKRVRELRFAITRAKDADTVMKRSRGDVERAIKSGVLDISIDVIQNPQEYMRTGKN
                     ILLHKGSYYRAVIDAEHETTAVVRVTQIEGEKAQYTCRVAWLDRASRYSRSPGSTMTL
                     PFDSVVEAVTFEEGVAESREIAARGVQSHQLSTSLSRDQFYDAIRSGVMRVTGKDRGG
                     WQGAPDVNYWAYRNANGQIQMAFTYGGMIGQKISASGSDEIAPEQWIYPDVNDEALKR
                     EVAKIQTGDNPLRISEAEGFLRALFGFDYEQGMEAWGAQATMDDVVKAFDGWMAGRDL
                     SGRSLEKVTQEDVRSLFRKPNGEGLETYWRGVLSFRDFQGRMKAFTNGSEISQMFEQL
                     RTSKITTKIEKTRMALNAWRDALFDQYKMQSDADGWASLASLADQSVYSAQLSISNAD
                     NPLETSATVWIKAGALIHAFEDKSITRDDFADADSLNRLVGMAIRHANALKAGGYVEE
                     LPDALKSATWDDFVSLKNSGATESEIAERLAAAKTEREEQADAAENKADEAANDDYII
                     VVNDKPIRAKARVRGRWWSVSEDAGAVYLIADMPGSSSIRNAKDAIKKIGGRFWNFEV
                     NPVADVDFDRPAWMVSTRYSIDEVRKIIEAAA"
     gene            17992..18426
                     /locus_tag="OQN12_001191"
     CDS             17992..18426
                     /locus_tag="OQN12_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="olxA"
                     /protein_id="HCS1653600.1"
                     /translation="MTTLIDTVKPTESYLEDILPQALNGRSEEAFLDAYLNGMVERLK
                     RDPQLYHLYGPWWPALKTLLLERGDISFGQVVDSDVAEIYKMSRPALTVLAGHLYSSD
                     RLENDAVYNPVHALEVAPYADDTEPYVYTSYDESIEKYRIMG"
     gene            18944..19321
                     /locus_tag="OQN12_001192"
     CDS             18944..19321
                     /locus_tag="OQN12_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCS1653601.1"
                     /translation="MEKNPDLIKRNHEHYEQVRVLDWMYRNMRYVYEHTHATPNGGLR
                     GIRTAIKMVAEGQKKGYPDLSIDLACGGYHGMRIEMKHGRNRLTPEQLVWMTRLTEAG
                     YYCFEARSAAEAIKAITEYVCLD"
     gene            19336..19536
                     /locus_tag="OQN12_001193"
     CDS             19336..19536
                     /locus_tag="OQN12_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653602.1"
                     /translation="MRLLNVAELIPAGTNAIARRRSWQQALCIEISKRINGSFEARNI
                     VTGDKVHITPESASADDWEFVH"
     gene            19946..21154
                     /locus_tag="OQN12_001194"
     CDS             19946..21154
                     /locus_tag="OQN12_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653603.1"
                     /translation="MSQHTDTSNLEIISTAIETLRTQIALIQKRNPGDDLSKRLHESV
                     IATTDNLVAEINKLLDDGAVDYNKLVDQFEEYQQAVNDGLIRFSQVTGVSATVESLGD
                     AVNQFAVNMRNEIGNLEARLEQANTLRKSAEADLNRYKKDYPPSLTKRLDVAEKDNRT
                     LKRERRELKERLTKLNQQCIDYQGEGVTLRKKLATAQSIIETLKRECSQLGHDLNRAC
                     GMGQRPETFPLMYDGRDAIAYIHEYPHGLVAETGQRGEALLTANYHQQIRTNRLLTMD
                     VIPSVWGTPLYYRLPGFEKDWNTDIDECLADKIMAYLETDFPRLYRRIMDSKDAPIDE
                     LKMRPETLEAIKQTEFDTVFSVACIPSCFHESIPFMQGDRRQEIIDACRVWANEWDKK
                     NGGVEDLYGK"
     gene            22379..22453
                     /locus_tag="OQN12_001195"
     tRNA            22379..22453
                     /locus_tag="OQN12_001195"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22411..22413,aa:Asn,seq:gtt)
     gene            22459..22545
                     /locus_tag="OQN12_001196"
     tRNA            22459..22545
                     /locus_tag="OQN12_001196"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22493..22495,aa:Tyr,seq:gta)
     gene            22550..22625
                     /locus_tag="OQN12_001197"
     tRNA            22550..22625
                     /locus_tag="OQN12_001197"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22583..22585,aa:Thr,seq:tgt)
     gene            23331..23417
                     /locus_tag="OQN12_001198"
     tRNA            23331..23417
                     /locus_tag="OQN12_001198"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23363..23365,aa:Ser,seq:tga)
     gene            23898..24725
                     /locus_tag="OQN12_001199"
     CDS             23898..24725
                     /locus_tag="OQN12_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1653604.1"
                     /translation="MKKTLISAAIIFGSLCLTGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDDPFSFQMSDGTTIGYHIGVAYKVDPAKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSNTAVPFVPVK"
     gene            24880..24955
                     /locus_tag="OQN12_001200"
     tRNA            24880..24955
                     /locus_tag="OQN12_001200"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24913..24915,aa:Ile,seq:cat)
     gene            25409..25984
                     /locus_tag="OQN12_001201"
     CDS             25409..25984
                     /locus_tag="OQN12_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653605.1"
                     /translation="MLMANDNAYAEEDLILSDFIGKRERWTQKREELYASLVRKGVNI
                     ETAQSGDMTVVSVGLHGVSVSAINHEPYVALSESMVRLVKFLKYTEANNVIIGKKNIP
                     FSSAFYWMMKGLDARRTSWPKGSYISMFRGSIGSKEKLFEFLPEEAFDIVEGCDVMVM
                     PRLVMMNGDLQAQTDWFATGVDIIATDWEAF"
     gene            25984..26376
                     /locus_tag="OQN12_001202"
     CDS             25984..26376
                     /locus_tag="OQN12_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653606.1"
                     /translation="MYGTTKLSTQQATNMEWSIANCKIVSCNATRTDVGHKSTITGYL
                     VMTTRGMRVSFRWKAEQVTPFREPSMPLQVEQDYLCLPEIDGGMLVESDGSKADFEAN
                     LKLAQAVINSCRGKWIAKVKHVVRLSRW"
     gene            26485..26877
                     /locus_tag="OQN12_001203"
     CDS             26485..26877
                     /locus_tag="OQN12_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late promoter activating protein"
                     /protein_id="HCS1653607.1"
                     /translation="MEQISSAEIADIMIRADCYLTVTEITILAKEKYPHLHVSRVSVT
                     NIIRHFVRSSRAICELDDRVYPRKYWLHGLNGYQFKVRGRTPEYGSLLVKNCSRKSVE
                     QARKEQRELVDMANKLWNAAVKKRGVAL"
     gene            27258..28238
                     /locus_tag="OQN12_001204"
     CDS             27258..28238
                     /locus_tag="OQN12_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA pacase A subunit"
                     /protein_id="HCS1653608.1"
                     /translation="MAKMIPEEDKSAPKIEGSSAMIPDGAVQRATLPTTNVARDMMKN
                     GAEEHLRLAIQMAQERALQYQSIVDQEAERLQAEIDTLGDKEPEGMHPGQRLLGLISD
                     AAYYMNDFISRLAAIYQSEQKLRQGDEKLRQSARQQAFKEAEAREKLELARLQAEQRG
                     KEIEYRIGADARAARVIAAAIRMREREELDDIGVAEYIERQGVSVPAILAARAAKAIT
                     LLEPPVSDVNDVDDEQLDKEAREFATLQANHPQWLAERRADVATIVEELGCGDYDRNG
                     ERKAGEFEANDEELDIDPSATAEIYGDYDASDAGYDAGDDDIAIEPPEDD"
     gene            28238..29746
                     /locus_tag="OQN12_001205"
     CDS             28238..29746
                     /locus_tag="OQN12_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCS1653609.1"
                     /translation="MAGGRKVKCVTRDPRWRDMVIKYRYNFTQAVVDIFGMFPSPQQQ
                     QIIQSVQETGSRTTVTSGHGTGKSSLTAMLLLIFMILFPDARVIIVANKIGQVKTGVF
                     KYVKQYWANAVKRHGWLQTYFVLSDTMFYERSRKGIWEVLCKGYRLGNEEALAGEHAA
                     HLLLILDEASGISDKAIGVMTGALTEEDNRMLMLSQPTRPSGYFYDSHHSRAKTPDNP
                     KGIWTAIVLNSEESPFVTPQFIKEKLLEYGGRDSIEYMVKVLGQFPREINGYLLGRDE
                     CDRSARRKVLLEKNWGWVATADVGNGRDKSVLNICKVSGHRDKRRVVNFKVMEMPGTM
                     DPLAFADFIYNECTPEKYPNITIAVDADGFGSDTCAQLVRRGANPVRIRWGKPMFANK
                     DRERFVNQRAYANIMARDAIKSGRMRIDSDPKTAEQASKIPFLMNEEGKMAMMRKEHM
                     RQKLNIKSPDRWDTYCFTMLVDYVPANEDIGAEMATFRDQVLADIEMPDLDI"
     gene            complement(29774..30811)
                     /locus_tag="OQN12_001206"
     CDS             complement(29774..30811)
                     /locus_tag="OQN12_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HCS1653610.1"
                     /translation="MSMKSIEKEYKRFLQTAERWKELVVANSVFHDTSYAGEEFRHVA
                     LTHDQNILEEAEKCLAEWKAFVDMCRDADGKASNIVESVYSPIPFIIEDTNQSTHVVV
                     QSATTTRAFTREQLLKKYDKIIKKSLKNRVFSQIVGALEEEQRFFEAEPEGEIYRARK
                     EAYTDVVLTTNIEGSNALSRFRVGAHGALVFARLPKTTIPVVNNVGERRSITIYSGVE
                     SVPCSLLGDFNLYRVRDLEKHQPSYVAKSYILRNIDIRNESLKQKSAKMLEDADPAIR
                     HIIERKIRTSREAMARLDKMDLELLDVMMASGDDLTGIKLNEARKKYGKAIEERYGYT
                     FPQTQYAAKLW"
     gene            31208..32236
                     /locus_tag="OQN12_001207"
     CDS             31208..32236
                     /locus_tag="OQN12_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1653611.1"
                     /translation="MSELIISGSSGGFLRNIGKEYQEAAENFMRFMNDQGAYAPNTLR
                     DLRLVFHSWARWCHARQLAWFPISPEMAREYFLQLHDADLASTTIDKHYAMLNMLLSQ
                     CGLPPLSDDKSVSLAMRRIRREAATEKGERTGQAIPLRWDDLKLLDVLLSRSERLVDQ
                     RNRAFLFVAYNTLMRMSEISRIRVGDLDQTGDTVTLHISHTKTITTAAGLDKVLSRRT
                     TAVLNDWLDVSGLREHPDAVLFPPIHRSNKARITTTPLTAPAMEKIFSDAWVLLNKRD
                     AAPNKGRYRTWTGHSARVGAAIDMAEKQVSMVEIMQEGTWKKPETLMRYLRRGGVSVG
                     ANSRLMDS"
     gene            32346..32654
                     /locus_tag="OQN12_001208"
     CDS             32346..32654
                     /locus_tag="OQN12_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653612.1"
                     /translation="MDGYTVTFAAGVKNSHAVVVFGIMTGLEAVPVFTLEKNWRNLEE
                     AESYVRKVTIIAAEKLLAHYQENYRTMVEKINLAFTRPTTGFIAPDTNAGRYPARGRR
                     "
     gene            32651..33127
                     /locus_tag="OQN12_001209"
     CDS             32651..33127
                     /locus_tag="OQN12_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653613.1"
                     /translation="MKTYYPSLNCYPSSYPGLSGKLLALLAKAPTEWFKPVVINPHFL
                     DYGSVRTRNELKNLLAHGFIRHRAGKGYQLSIAPEEAVRLFAYRDSQTKARVIAQILH
                     NGSTYARQFGGDTSQFLRAVRSLEEQGVIESSCIPVPTVPHIKRRVYTFTQRAKKQ"
     gene            33124..33618
                     /gene="dut"
                     /locus_tag="OQN12_001210"
     CDS             33124..33618
                     /gene="dut"
                     /locus_tag="OQN12_001210"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1653614.1"
                     /translation="MIYVKVKRLHPAAKLPAYATSGSAAMDFEAVEIKPCVDSNGAIS
                     SSWWVYTGLAMEIPPGWCLKLYPRSGLGCKKHTRLANCVGIIDSDYRGEIMAKLITDP
                     GGEGVCLKPGMAVMQGIFERVEPVSLVEVDELDETERGAGGFGSTSEDRVEIKRTPLQ
                     YAKF"
     gene            33633..34295
                     /locus_tag="OQN12_001211"
     CDS             33633..34295
                     /locus_tag="OQN12_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCS1653615.1"
                     /translation="MVSTFQVFPKYKCHSIIRASKIKDIVIVGKAYAPIFGSIEVVEP
                     KGMTLRVDGVFLEKFRPQIGGYLVTAENGYLVYVPEKAFEKCYSRVNDASENGVSLSI
                     EGHNGVTFITARDVTISAGGIATLQEEIDLEAADFSDALMWLKDGKKVARRGWNGENQ
                     FCWLVPEGQYPARMEAIKGYFPGDLVPYGAYFALKNAQGVVVPWLPSVGDLLACDWFV
                     VE"
     gene            34302..35102
                     /locus_tag="OQN12_001212"
     CDS             34302..35102
                     /locus_tag="OQN12_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653616.1"
                     /translation="MENTKAIQYRLRNGLLVAVNADMSLPFDTIERTIMTYLGFNEEL
                     NEEHGVAIWSDAESGVHRYITARGKDYSLEELFTLAQSFECVALDMFNDPAIAQRLIR
                     ELGLSVTPIIFKNGSLTGTWRVERISNYLPYNRQLNGVISGVNQPVACENVNLVAAVL
                     ATACRVIGLAKQAFIHFPNGAEGSAEIIACDFEFTWMLREYLDQTVFRAEELDMYITS
                     MIPDDVRAEAIATARAKCRAAIAEQAKEEPSDEAVADVEAEVAEDADA"
     gene            complement(35186..35566)
                     /locus_tag="OQN12_001213"
     CDS             complement(35186..35566)
                     /locus_tag="OQN12_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCS1653617.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGIQVYDSPVLVELAV
                     GAATGEIPVSSVAEKLRELFGSNI"
     gene            complement(35566..35787)
                     /locus_tag="OQN12_001214"
     CDS             complement(35566..35787)
                     /locus_tag="OQN12_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCS1653618.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            complement(35860..36249)
                     /locus_tag="OQN12_001215"
     CDS             complement(35860..36249)
                     /locus_tag="OQN12_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HCS1653619.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGRKEALPVDDDGCT
                     GSNAVFGVITHVINDVRSGEFDDCPVI"
     gene            complement(36348..36599)
                     /locus_tag="OQN12_001216"
     CDS             complement(36348..36599)
                     /locus_tag="OQN12_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1653620.1"
                     /translation="MSDRNIAAKSQEERDKVNVDLAASGVAYKERLNIPVIAEQVARE
                     QPENLRAYFMERLRHYRQLSLQLPKGSDPVYQKEDIAKK"
     gene            36773..36982
                     /locus_tag="OQN12_001217"
     CDS             36773..36982
                     /locus_tag="OQN12_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653621.1"
                     /translation="MRFILTLKSETEGIKHSVKVFSEAILLNGKLRHLVRPERQYPDV
                     LAREKDLLSEVQDRVIDFVKCYPLH"
     gene            complement(36967..37251)
                     /locus_tag="OQN12_001218"
     CDS             complement(36967..37251)
                     /locus_tag="OQN12_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin PHD"
                     /protein_id="HCS1653622.1"
                     /translation="MPTKAELQVRVDELEKENASLKKMLSRAERELSGKLLPEELPPA
                     DIPDRVSWWMKYFRAPWEAFWCYDHRRWCDELDSSFPYFAEGNTCPQCRG"
     gene            complement(37235..37936)
                     /locus_tag="OQN12_001219"
     CDS             complement(37235..37936)
                     /locus_tag="OQN12_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653623.1"
                     /translation="MTTITREQALKIIEAADEVISALAGTNEDVHPGSDNMLRLWDDL
                     NDRYAPPEVVRELARIALASLEAEPIGEVSEKRHGLVMDGTVDLGGKSTYRIIKGEKA
                     MKLLPLGTKFYIAPPAPVIQADVAQSIENLKQKLVECNRYNYCADAVKGVEDACRAFM
                     LQSGNFRESDVITPDHDKQAEKSGLERCHLETALNIKPGHTLGIIDALLVHKMARALL
                     PLVAEKHEADHANES"
     gene            complement(37933..38142)
                     /locus_tag="OQN12_001220"
     CDS             complement(37933..38142)
                     /locus_tag="OQN12_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653624.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            complement(38378..38854)
                     /locus_tag="OQN12_001221"
                     /pseudo
     CDS             complement(38378..38854)
                     /locus_tag="OQN12_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(38841..39530)
                     /locus_tag="OQN12_001222"
     CDS             complement(38841..39530)
                     /locus_tag="OQN12_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653625.1"
                     /translation="MKERGMIFNDEMVRAILEGRKTQTRRPVKNVGSDNCLVIRKPTK
                     KRNGVYTHVMDAPEHGLCPFGNVGDRIWVREAWAILGNEDGCSVDWNDNLCRGDEKNA
                     ARIYRASCEQKPGDYGLWSIPDDADWKPHTVNEKFDGGWRPSIHMPRWASRILLEITN
                     VRVERLNDISECDARAEGAPTESTLIGDKHYPGFRSLWKSIYGEDSWNANPWVWVIEF
                     KRIQGATSEQD"
     gene            complement(39547..40542)
                     /locus_tag="OQN12_001223"
     CDS             complement(39547..40542)
                     /locus_tag="OQN12_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1653626.1"
                     /translation="MSAIITPHFVNGAGVAVFPVDKPTSNYIGAGRRFIISPLPREQA
                     ENTPDGVVDLNYSLVANQSLKAFFQSERVFNALGGEDSLVHWVSSNIHDCQAHDKRDC
                     SHQLTTHFYNGSAVRLCWKHDAEYMMKGYGKLDDQLSLNRANWVMNWAASELKLPPER
                     DLSMVELTFWAIRRNLKDELPDEAGRIAFCQPKQEIPTGTLKESDITWEHSTRELVDI
                     TAEQIVNLSVDEDSGLLYMRRPKAVLGKSPAYLRFVVSRPCIGCGGKVNHPFMYRARS
                     LNEHDRWAVPLCDDCARSAENDVRAWEKAHGIRLYVAANQLFDFAIERGVITFNN"
     gene            complement(40641..41333)
                     /locus_tag="OQN12_001224"
     CDS             complement(40641..41333)
                     /locus_tag="OQN12_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCS1653627.1"
                     /translation="MNQALHEVNLYSRIDGSGYRNIWVVGDLHGCCTLLMNELHRVDF
                     DPTQDLLISVGDLIDRGTENVECLELLQMPWFRSVMGNHERLMIDALSPAGNVNNWLM
                     NGGQWFFMLDTDQEILARALVELVKRLPYIIELNTGHETIVIAHADYPGGEYQFGKDV
                     SLFDVVWSRSRVGDSIDGIGGEITGADRFIFGHTPVRRPKAYWNQHYIDTGAVFCGNL
                     TLMQVKGGQLKV"
     gene            complement(41330..41641)
                     /locus_tag="OQN12_001225"
     CDS             complement(41330..41641)
                     /locus_tag="OQN12_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653628.1"
                     /translation="MNTIKEMPVERDEYGCWTHPEYEKFCAGREYISTEEFDAWMKEN
                     NLQWTIRSMDEDHFNLDADGPDIAAWEPERPEGEGWFVGSIHDTEDGPVCIWLREKVA
                     A"
     gene            complement(41638..41862)
                     /locus_tag="OQN12_001226"
     CDS             complement(41638..41862)
                     /locus_tag="OQN12_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653629.1"
                     /translation="MTEQTMTNRELVDAAIELAGDFYSMMGYTHRPGFKYWESPHPQE
                     QRVFQMACRAFEVIRGSDVMDAVADLEDEE"
     gene            complement(41859..42134)
                     /locus_tag="OQN12_001227"
     CDS             complement(41859..42134)
                     /locus_tag="OQN12_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HCS1653630.1"
                     /translation="MNIDTTITIDTLLNTGLALLGWFFIMFSAGKWAASVFLKQWGKR
                     RKQEKRQKALEAFYDAFELGSIEPGTTARIATKGDLMIVMFRQEKTR"
     gene            complement(42131..42826)
                     /locus_tag="OQN12_001228"
     CDS             complement(42131..42826)
                     /locus_tag="OQN12_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCS1653631.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGSIGSTPSVEV
                     QSIHAGFDWDSGKVLIFPAQPLTTLTPEQITDITDSVRKGQPWHAYQEYKKHKEQLEK
                     LSIELDAAKQRIAELEGNCAALAAENAGIKSAIPESRDIEDDNDNMDDVSLAEDFGFN
                     HAIERMRRQIPETPTTDAFLAEVRAQGLEMFAQKCNSKSEQSLASDIRDNWKLLGEHA
                     TDFADELRRGSSQ"
     gene            complement(42823..43185)
                     /locus_tag="OQN12_001229"
     CDS             complement(42823..43185)
                     /locus_tag="OQN12_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HCS1653632.1"
                     /translation="MKSEGLTPAQLAERNAELLRITQEKAFHILERLETLSTEEEISP
                     EMLVECSRVILRRKNDIARLTSGAPSVSASPTLYCSFCNKSQHTVKKLIAGDNAFICN
                     ECVKDCNNIIQEDQRESA"
     gene            complement(43175..43459)
                     /locus_tag="OQN12_001230"
     CDS             complement(43175..43459)
                     /locus_tag="OQN12_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1653633.1"
                     /translation="MILRQCAGTMTVESIGKLIGRTGDAVRTKARELGISMILKGDFH
                     PSAKYRQSDIELARQLHQCGVPRREIAEKLEMPLGMINQYVYFERRVHEV"
     gene            complement(43489..44100)
                     /locus_tag="OQN12_001231"
     CDS             complement(43489..44100)
                     /locus_tag="OQN12_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCS1653634.1"
                     /translation="MSKRIMKIYIAGPMTGYPDYNRTAFFSKAKELMEVGHIVLNPAL
                     LPAGLCESEYMDICLAMVRSANAVYLLKGWEESVGARAEHELAQKLGLNVIYESPTNI
                     ECQVAPHIYRELVNALRDIAAVYHGTEQLRERLAHTISYYLSLSHEHKLRQKVMIKFI
                     MRLSKSLANADPKNPLPKEAMNYLKSCNVVSEDGVLLVRRSSA"
     gene            complement(44100..44255)
                     /locus_tag="OQN12_001232"
     CDS             complement(44100..44255)
                     /locus_tag="OQN12_001232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653635.1"
                     /translation="MMLVAISAIHQQSGSEIGLLYVLFFTVLALFELGIELFALINFC
                     VTFLGKL"
     gene            44372..44947
                     /locus_tag="OQN12_001233"
     CDS             44372..44947
                     /locus_tag="OQN12_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCS1653636.1"
                     /translation="MKQSYWEKQTQKALQKLADPKWREEQRAKRLQQAQRQQQRAREK
                     AASPEYRQKKIEKAKQYEQRRKEKAASAPVKKTRASRGLKGRTLTADERRIQTAIGAL
                     PCIACHMHGQHSPVVSLHHIFGRTAENAHKYVLPLCKWHHQHAAPAEIREQYPWLVPV
                     HADGKIGGKADFRRHNADEMTLYQMAIELMN"
     gene            45279..45446
                     /locus_tag="OQN12_001234"
     CDS             45279..45446
                     /locus_tag="OQN12_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653637.1"
                     /translation="MTVRYLNFQIQNITGGCYDWFVTLGKEVITGKLDEVKAKAMAYA
                     CKQARKKSAKA"
     gene            45570..45749
                     /locus_tag="OQN12_001235"
     CDS             45570..45749
                     /locus_tag="OQN12_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1653638.1"
                     /translation="MATIPTPTHSEFIWRFYSCQKHLYICVMAATEAEARSYLPLAER
                     GVIGDKQILGNSGTT"
     gene            45709..45837
                     /locus_tag="OQN12_001236"
                     /pseudo
     CDS             45709..45837
                     /locus_tag="OQN12_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717724.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(45834..46061)
                     /locus_tag="OQN12_001237"
     CDS             complement(45834..46061)
                     /locus_tag="OQN12_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653639.1"
                     /translation="MSYVFQEYAEMGGTYTLYSLDVPSRGDMTLSHQWQNADGEALRE
                     VKTEKCGTFHSFKGKAPNVKSVLEKQRAGEL"
     gene            complement(46247..46855)
                     /locus_tag="OQN12_001238"
     CDS             complement(46247..46855)
                     /locus_tag="OQN12_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653640.1"
                     /translation="MKLRALGAALAAMLGCVSANTANATALPAQFRAGQQVMNNAGGD
                     HSQAAIMDFCKREGIPLRPVGTQFIGKTDFCVFAYTAYLTDKAITKTGYSTKDTLSRL
                     SQGWQQFEVYRQQGLGELLQPLFMLALVPEGQQFLVKKGMLRQSDIAGFDSMMAYERK
                     LTEQRNKKPSASCVQSKTAEYSAVAGPLAKQMAEQWCKKYGQ"
     gene            47139..47792
                     /locus_tag="OQN12_001239"
     CDS             47139..47792
                     /locus_tag="OQN12_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HCS1653641.1"
                     /translation="MEKIAIFSLTTSKPQMLTAILKDGALIINEVKPLPASALEQKQK
                     IPPAIAALRKSKFKVLVDEITPTISAGTGASQVTLKTRHADGRAAIIVGMERYRELKL
                     QKLLSLPQNNKGAFEIPDSIVDTEYNGNGEEVYRVNWQDIRPEHILMILCCYATVYHN
                     VASADYVEQMTGTVEKEQKTGILASFLSIIGHEKVKAGTSQPKTLTGKEVDEDTVIL"
BASE COUNT        12962 a        11295 c        11975 g        11864 t
ORIGIN      
        1 taggattgaa atgatgacgc cggaaacttc ttataaagcg tggaaacagc cacatcatag
       61 atgattgcaa cctgcttacg ggggatgccc ttctccagca atcgccgcat ttgctgccat
      121 gtttcttctt ggtatttagg ccgacgccca cctatacgac cttctgcgcg agctgcatca
      181 agtccagcgc gtgtacgttc aacgataagc tcacgttcca tttctgccag cgcccccatt
      241 acgtgaaaga aaaagcgccc cattggtgta ctggtgtcga tggagtcagt gagactccgg
      301 aagttaatgc ctatgtcacg aagctcttcc accagcacaa ccaagtgacg catactgcgc
      361 ccaagacgat ctaacttcca tacgactagg gtgtcacctc tggaaagcat acggagaacc
      421 ttttttaacc cagggcgttc agcctttttg ccgctcgcct tatcctcaaa aattagctca
      481 catcctgcgc tttcaagggc gtttcgttgt aaagctgtgt tttggtcatt agttgatacg
      541 cgaatgtatc caataagcat atgttccccc ttgtatgtta aggagggaga ttttagaaga
      601 tgtcctgtaa ggaaataaca aactgactaa gttagtggcg cattagatgt tatttctgga
      661 ggggtatgtt tgcagattat gaaaatctgg ctgtagtagt cataacatcc ctactaagtg
      721 gaaccggcgt attccttctc ggagttagag acgggcgcat ttctgcgtcc ctgctcaatc
      781 ttgcgagtga attgtttact gcggtaacag ccgggcttgc ggggtatggg gtggcggtta
      841 gccaggaatg gcctgaaggt atcattttct gcgttgttct gattgccagt aacaacggaa
      901 gagaaatttt acagggcttg aagtccagag ctagtaacgt cttgaatctc ttaagcgtaa
      961 tagcgaatgg gggaaaaggt ggagagaaat aatgggtaac tttgggaatt atttcatttt
     1021 tatcatggca tttgttgcca tcattgaccg tttcattttt cgtcgcaaga gtgttgagat
     1081 tttgagtatt ggtgacgctg tagtgaaaga atccgctata gcatttcctg tttcgctacg
     1141 tgttaagcga agctgggtca gcaatgctca aatcgaatat tggttgcaag acctgaaaaa
     1201 tccatctgtg gttatttcag ggaaaacgcg accagttgac gcctcaaaac gcggtgagag
     1261 agaagagtat ttgctcatcg atacgaaata tcttgagcca acgaagtggg atctgaaagt
     1321 cacattgact aacggtaact gccgactgaa tccgctttat cgcattttcc ctatcaatga
     1381 ctgcatagaa cgccagttta cgatcgaaaa gcgtgaagga gactgggatg tcaaataaga
     1441 ataattatgt tcttcttaat tatgatgagt taaacgaaaa ggggctggca aaactcgtca
     1501 aagaaatcag caaaggtggt tacaagatag cgagggtggt ccctgcaagt aatggcagga
     1561 agaaagatgg gatcatgacg cgcacgttta cccttattgg tcttgatgaa cagacaatgg
     1621 aagttcaggt taacgatacc ggtgatatat ctgggatcaa actgaatggt aaaacagtac
     1681 cgttcaagcc agtaaaaaca atgtcggctt tggggcaatc tcttgcggct ttatttaatc
     1741 gcggagcaac ttctttccag aagtcattag ctcgaaaact tgctcgtgca gcaaaaaata
     1801 ttgatgacga aaataagaag cggcagggag ttaaatccaa cgctcagaga ttagctgaag
     1861 cgaaagaatc acgtgatgca ctccgtgaag atatcgctaa tgctaacgac aaactgtcta
     1921 aactccagag caagtcagac aaagtaatgc aagatacggg taacgttaaa gctgccttgt
     1981 ctcaggaagt agctaagaca cgtgctctta aagaagaaat cgcgcgcctg gaggatgagc
     2041 atgattaaca atcctttacg aattaatatt cagtctactt ttgctgatac gatccccggc
     2101 tatatgccag gtcgctggtc ttcagaagaa ttaagcgaag atgacctgat ttttgaggcg
     2161 gcgaccttag aagatattga gcttgcctat atgggcaatg aaattctggt tgtgtctgac
     2221 gaagctatgt ttgagtccat aactacaacc agaattcggt tagctcaaac aatgcgagct
     2281 ttcgtccgtg cgctgaatcg tggcctgaat ggtacgaata ttatggcagg gactgatgaa
     2341 gccgggaccg atgaaaatgg acgtaattgt attggtggtg cgattatcgg tcgtgtacgt
     2401 cgggtagcta atatccctgt tttgaccgcg cagataccgc ttactgacgg gcagagtaca
     2461 agcataattt tccactctcc aacagctgac ggggcaaaaa taaagagtaa cgacgagctt
     2521 gttgcatttc agtttttact taataagcgt aacgtaactc acgttgtcgc cccgattggt
     2581 gggagagatg tttctttaag ccaggtatgc caggcgctat ctaatttgat tgagcgtaac
     2641 agcgaaaagt ttaagaaagc aaaagagcgc caggacaaaa tgaaggcaga tattgagagt
     2701 tatctcaatg aagcagataa actggcagaa gaacggtcaa taacaattga tcaggtggac
     2761 gttgcgcaac gtgggctgac tgaaaagcgt gctgctctta cggagatgca aaaaaaactt
     2821 gatgcccaga aagccatcaa cgaggaatta caagaaaaac gcgataaatt gttgtctgct
     2881 aaaggtgaaa aaacgaagga gcgagcgttt agtgaccagc tgcgacatgt aaaacataat
     2941 cttgccattg acggaaaaac agtgctggat aatggcgctg aagttaatta tgtcacgtcc
     3001 ggcagtgaca atttcgttac gatagtagca cctgaaggta aattcagtat tgatgccagt
     3061 gctgtcaaag gaggcagttt ggcagatgct gcaaccaaat tacttaaagc ctaccgagag
     3121 cataacgcag ataaatataa agttgatgtg ttaccaggcc agcaggaaga ctccgaacca
     3181 ccacagacag cccccgagac gaacccagta tcacaaccag agagccaaga tgtaggtaaa
     3241 taccactatg cacttcaatc tcgcccggct ggtgttggtg ctgtgccgga tggaaataaa
     3301 gccgtgcttg atcgtcctga ccaggctgat cagtattacg gatatgctcg tcacggtatc
     3361 attacgtatg accgtaaact gacagataaa gaagtcagcc agtacgagtt gctctatctt
     3421 cctgatgaag atgagctgaa agattttgca ggccaattgg ttgtgtccag catgtctaaa
     3481 catattgatg gctatgtgga cctgtttggc agtgacctga aaacattcaa ggcgcaagtg
     3541 aaaattcttt tccgcaaagc gtttccaaac gtggcgtatc cacttggtga tggggagaac
     3601 cttttcatta tggacgtgta taacgccctt caaaattatt caagcgaagt aaaaacagag
     3661 catgtggttg agccagaatc acaaccacaa ccagcaccag aacaggagcc ggagccagaa
     3721 gaagcgagtg aatcagcaag tgaagctgat caggaggcag ataaagcgct ggagtacctg
     3781 aaatccgtgc cggagcagtt tacatcccgc gatctgacgg tcatcagtac tgaacttgat
     3841 catgtacagg aagcagcaaa tgctctaatc agtgcaggtc gatacgacga gaacgaagcc
     3901 actgtgggtg cagcagttga ctacctgatc aacattctgg ctgaaattca gcagggaggt
     3961 gcttaatgac catctctgtt ttagaccgcc tgaaattagg taaagagcta tcagatctaa
     4021 tgcaggcgca gaaaactgcg ccagtattgc aacgcgtcac cattggtaag cagattgttg
     4081 atgtgatgct gaaattgggc ctggggactg ccgcgcaacc ggcagcggaa ccgcaacaac
     4141 agactaatcc tgttgtagat gaagtcccta agatagtgac tgacttcctg ggcggtgtgt
     4201 tcacaaagtc cacacaaatg gaatttattg atgcgctgcg tgggatctca aactatgttg
     4261 gcgagttcct tacgttggag caggctaaag aacagaccat aagctgggtg aaagccaacg
     4321 gttatgccgg gtaatcaaaa gggggtgaaa tccccctttc acttctctgt tctcacataa
     4381 atcacactct ttttacactc acagctttct ctatagttgg agtgtatttt gaacaaatca
     4441 gttacttctg cgctttccga ggccgcagat attaacagcg tcattgctct ggtttcttca
     4501 ttagagagga aagaaacacg catggggcga agtagctacg tagtcaccag caaaggcgca
     4561 gaggtaaaga cggcttttaa ggtcgttgat gccagcagtc taatcatttc gaacaacctt
     4621 gacggtacga ttaacccggc cttcccggaa gaactccaac cacgagatcg tacccggcta
     4681 tccagcaaac tccaggttaa ccgtattgcc tccgatttac gcccggcaca attaacggat
     4741 tccggtatga gcagccatgg agcgccgata gttggtccgg acaacgttgt tgaatccggc
     4801 aacggaagga gtatgggtat ctggcgtgcc tatgagcaag gacaggcgga tgactatcgc
     4861 cagtacctga tcgaccatgc gaaagagttt ggtctgaatc ctgacgaaat ttcgcaaatg
     4921 tctatgcccg tactggtgcg agaaagactc acagatgtag accgcgctca atttgcccgc
     4981 gattcaaaca ttagtgatct gcaagagatg gcagcaagtg aaaaagcgta tgcggatgcg
     5041 caatttctca ctgagagcgt catggcgcta tttaatcctt cagatgatgg aaatctgctg
     5101 gcgcggtcca atgatgcgtt tattcgtgcg tttttgcgcg aaattggcga tacagcgacg
     5161 gcaggcttgc ttactgccga tgggcgcccc acgaaacagc ttatcgatcg catccagaat
     5221 gcaatttttg ctaaagcgta caaggatgaa aggcttgttc ggctggtgtc ggaagagcca
     5281 gacccggaaa tgcgtaatat ccttaccgca ttaaatacgg cagccagcga ttttgcgcaa
     5341 atgcagtcgc tgtctggcga cgctcaccat gacgcagtaa caggcttagt ggacggtata
     5401 gagcaggtta atggcctgga tacacaggcg atagcagcac tccaggaagc aattaatctt
     5461 gtacgtgaag ccaaagacaa cggtcaggcc gttgaagaag tgatcgccca aagggggtta
     5521 ttcggcgaca gtacaccgga agctgaggca ttggcgctat tcatcgtagc aaataatcgc
     5581 agtgcgaagc gtatgggggc agcattcaaa aaaatggcgc aaaaaatcaa cgacgagctg
     5641 actcataaac aacaggcatt gggcgatatg ttcggcggtg gcgatgttga tctgcgtagt
     5701 attttgtctg ctgtatctga tgaaattgag ggtgaatttg gcgaagggaa aggtctgaac
     5761 tttgctatgt ttgaaaattc tacagttcca aataattgcc gtacagaaaa ctaaaccaac
     5821 cgctgaaatc acatactgat tatgctgccg caaaactgga aacaatttgc ggtgattcat
     5881 ggcaacgaaa gacaaaaaca aaggctttct gtcagcattg aagaaagcct tcaacggtgg
     5941 tgatgttacg cccgccgatc cggtcgtttt tacgagcgga catagtgtcg ttgctcgttc
     6001 cggcctgtct gctttacgtc caggcatcct ggggagcaat agcgatggca tgacgagtga
     6061 ggcagattca atttctttat ccgctgaact gcccggcgag cgcctgcaaa agtacaacat
     6121 cctggaaacg atggctaaaa gcccgactat ctcgacggct ttaaacattc acattgcaca
     6181 tgcgcttgcg ccttcaaaaa aaaacggaca ggcattcatc ctttcgccaa aggatggttc
     6241 cgatgccgag gcagtaagta gatgtgaaga gttaaccgct gatttggggg cgatgattaa
     6301 tgacggactt ccttcatggg ccatgatcat ggcgatcttt ggggtgtctt atgtccgacc
     6361 ttatgctgaa cagggcaggg ggataacagg cattgagtcc agttattaca ctctgccaca
     6421 tttcgtccag gaattttatc gcggcagcca gctggttgga ttcagtggtg attacatcct
     6481 ggatacgcat agtttgcgca gagtaataac cgaaccgtgg aatctggttt ccatgaaaaa
     6541 tccctattgg acgccacagc ataaagttat tccggttagc tacggcacaa aagggtactc
     6601 actgttaagt gatcaagctg ataagccgtt aatggagacg cagaattacg gaacttcatt
     6661 cctggaatac agctatgaac cttaccttaa tttatgtgct tcgctcgccg cgctgaaatc
     6721 aacgcgaaat aatgcagcca aaattgaccg cctgattgct cttacgacga acacacttga
     6781 cccggttaat gcggcgaact atacgcgtgg cgtcagccag gcattgaagc gtcatagtga
     6841 cctggtggcg caaaggtcga ttaatgccaa cgctattcca actgtgctta accatctgat
     6901 acccgtcatg ggggatggga agaacgggat caccattgat acgcaatcta tacccgcaga
     6961 tatcagtggt atcgaagacg ttatgtttca tctccgacag ctcgcggcaa gcctcggtat
     7021 tgacgcaaca atgcttggct gggctgatca gatgagtggt ggacttggag aaggcggttg
     7081 gcagcaaaca gctatccagg ccgccttacg cgcgaactgg atcagacagg ccgcccagcg
     7141 aactatttat cggcttctgg acattcacct cgcttataaa tacggcaagg tttacaccga
     7201 aaaaaaccgc ccttatgacg tgcaattcaa ctcaatgaac actgctattc aggaagagga
     7261 aaaccgggaa ctggatgcac gcgccaactt tgttgctgtc atctcccaaa ttatggacca
     7321 gatccagaac aacccgaaac tggctggtag tgacgcgttt atgcgttatc tgttcaccga
     7381 gcagttgcac atcgacgatg acactctcaa caccatgatc aaagagttta aggcgaatga
     7441 atcagagcaa aacaacgagc atggtatgta cgaatccgcg ccgttgtcgt ccggtgacga
     7501 tccggaaaac tggacgccag aacagttaat aaactttgca aaatttgtga tgagcaatta
     7561 accggaggga aataatgcaa tcactgaaca ctgttacgga tcgcttctcc ctggtagaga
     7621 aaatccgcaa acacacaccg cagaacaacc gaaattacgt tattcagtcg gtgcgagaca
     7681 cattcaactc gccagaaaca aaagagcgta tcgcacttgg tgagatgtat ggctattacg
     7741 gtcatggtcg ccgggcgatg cactataaca aaacgaaaag cctgaacctg ccggaagtct
     7801 ctgttgtgat ggttgatggg aaacctgttg tattggagaa cgtgccatca aacaggacta
     7861 tcgacatttc gattgacgac aacggcattg ttacccatac gcaggaaata ctggatacag
     7921 acaccgggcg tattgtacag ggaatgatta attcaggtgc tggtggttgg tcatgggcta
     7981 catctggccc agattcatct gtatccctgg taaagtcttt tcacggcttt gattacgtaa
     8041 ctgtgcctaa ctatatcagt ctggacaaga aaagcctgat gctggaatca gcagaagagc
     8101 gcgacgcggc tattcatgcg gcacttatcg aacaaggttt taccgataac gcggctacgg
     8161 atcttgttca ccacttttca gcaatgaaga atcagcaggc tattttcgag tcagcacagc
     8221 gtgaagccct tgagtcagaa ttgatgctcc ttgaagtgga aaacatacag cttcgagata
     8281 agctgcgcac tcaaacagcg atgatggaaa gccaggggga aaatgcaaag caatgccgcc
     8341 ggattttgcg tgacgctatc caggaaatgc ctgtttttat tagcgcagaa cagcgccggg
     8401 cgctctgccg tatgcagtct gaagatgatg ctcgcatagt ggctgcgatg cttgaatcac
     8461 tgggggcaaa tgcgacttat ggcttgccga tattaggcaa gaaagagccg gagttatcgc
     8521 cgaaggagag caaaaatact accccattac tcttcgtttc acgacgggca taaccgcaga
     8581 atgaaattaa atttctaaca tagtgtattt atatacactg ttgattctgt ccgatgtgat
     8641 cgagtaggat cgcggtcact gctccggtag cgactgccaa aaaaagcaaa agccgctgag
     8701 aagcggcttt ttggtgtata aatccactgg catagattta tacggtagat agattttttc
     8761 acggaactat ctgatgaata ttttaaattc agtttcagta actttcaaca cttctgagta
     8821 taaaaacaac actgaatacc acaaaaaaat caaaggtcat cagttgagtt tttttgctcg
     8881 tcatagcggt cttctttccc cttcacatag cgctattatt gccgagttcg ccaacctggc
     8941 aggggctacc gatgaataca tgatccgtcg ctcatatgct gatatgtccg agattacagg
     9001 acgcagtatt tctactgtac gccgggcatt tgctgaagcg acgaagtgcg gaatgcttgt
     9061 taggcaacat caggtagcga ataacaatgc ccaggtgtgt aacgtatatc ggtttacgac
     9121 acaatttctc cacttcattc atgcagcaat ggagataggg ggtaagcagg gcatcaaatt
     9181 tgccaacgcc acaaaactgg ttaagcagtt gatttcaaag gttcgttatt tttttgaaac
     9241 tgggaacccc ctgttcattt tgaacaagtc cccccatgtt caaaatgaac agccaataga
     9301 aaataagagt cactctatag caaaaagaag agaaagatca tgcgcggttc agccaaaggc
     9361 ttcaccagca gatagttctc aagcggataa tggggtgctg gataaaaaac cagtaatcag
     9421 ggaagaacat accaaccgct gcctggcggc ggcaaaagcg agagctgcaa agcgtcgttc
     9481 agacgagggg cacgcaaaac ggcaggctct gtatcgtacc gctgaaaagc tggcaaaaaa
     9541 atttgcatgg atacgtagtg ccgctaccgc agtgaataag cctaaacaat ccagtgccct
     9601 ggatttctca atggattatt ctggctccca ggggtgtgca accattggtg aagcctttga
     9661 cctgatgaaa cagcgtggct atcggtctga atttgaccgg gaggattggt caatccctgc
     9721 gggatttagg ggatgacctg tgtgcctaca aaacatccca atgatcggca aatgctcgct
     9781 cgatgatttg tattagaaaa ttaattttct gtttctaaat cggaatgtat gatctgcatc
     9841 atcgactgaa gtcatccatt gctgaaattt ttagttgatc ttccatgttt tcaggcttat
     9901 attcatgccg tcgtagcaaa ttctgcgacc aggtttgaca gcctgaatgt tagtgcggac
     9961 aaccgcagat ttccgatatt gcggtatttt tgtgtccgta aaccgcgtta cgcccgaatt
    10021 atggtggggc gtgatgggga ggcttcggcc tgctggtttc actaacgcca gtctgtcaac
    10081 cctgtcacgt cctgccacct gtttgacagc gggtagcagg ttgttaaacc tgttagtgag
    10141 gccgtaacta tggttaatgc caatccttgc tcacgtccag aattcatctg gcgtttccac
    10201 tccctgcaaa aacactgcga tcacttcgca attgccgcaa ccgaaaaaga agcccgttct
    10261 ctgatccctg atcagtatgg tgttcttact ggtcgtttct ctacttcccc acaaccaaac
    10321 actaatccct ggtatcgcaa tattaccaat cccagcattg cggaggcaca ctgatggact
    10381 ccagatccgg gaagttgatt tatcgacggc tgatatagtc ggtaatgatt ggatcgtgac
    10441 ttgaagcgtg atgccccggc ctggtcgggg cttttttcat gcctcattag ttgaggttgc
    10501 tgcgtctccg actggtaaca gagcggtatt gcgtggttcg ccagcaggcc agcgatagcc
    10561 agacactctt gatgttggga atgcgcgaat attaaccgca tccccctggt tcccacccag
    10621 gacaagcaga tcgccattgg cgcggcgtcc gacaacgaag cctacgtgcc caccgcctac
    10681 gcgggtaaat acaacgatac atccgtatgc cggtgtatct aatttcgcgc cccaatccag
    10741 ataggatttt gccgactcaa atcttgttga gcggatgcct acacgttcca gcattgcacc
    10801 gacaaatgct gcgcaccacg gcgtttcatc gtctttaatt ccgccgcgct tgatgtcgcg
    10861 ccaaaactga acgatttcag ggttgtgttt agcgcctttt atttcagtca ggccaatgtg
    10921 cttacgtgct tcaattagcc agcgaggttc aatgtttgcc atattttttt tccggtcatt
    10981 ggttaataag caggcaaact atggggggta tgtgattttg acggtagtat cgtccccgtg
    11041 aaaataacat tctccatacc ctctcaaccg taccgcatag cggatttttt acggtttaga
    11101 gggggtaatc ctttggataa actacttcat tcaatcagtg aagcctccag cttaggcgaa
    11161 ctgattgaaa ttgttagtcg gttgcataaa ttacgtgctg ttgcgacctt tggcgttaag
    11221 aaagtcgatg gtgtcagcat tcaaaaaaca cggcgggcag caaacaacgc cgcagttgag
    11281 cttcttaatt cccttccgcc cggcttcgat ggggcgaaat taactgacga acagcgtcag
    11341 attcttgccg ggtataccgg tgaaggcggc ctgacagatg gtgagggcag ccaatacgaa
    11401 tactacacgc cacagtttat ggctgaaggt atatgggatc tgtttgctga ctacggtatt
    11461 gatggcgggc acgtactgga accatcagcg ggcacaggta ttttccagga gacaaaacga
    11521 cagggcatga taatgaccag cgccgaactg tctccgattt ccggtcgaat aaaccagctt
    11581 ttgcacccgg aagacgatgt gaatatcggg gcgtttgagg cgctggcggc gaaagacgca
    11641 atgtatgatc acgctgttgg taacgtaccg tttggtgaag ggcgttccgg tgtcgccggg
    11701 cttgatccgg catatgcaaa cgagaagaac gttggcaatt actttgtgct gcgcacgatc
    11761 gacaaggtta agcctggtgg gcttatcgta ttggtggtgc cgaatggcat gaccgatggt
    11821 acgaaatata aaaaattgcg cgataaagtc agccgtaaag cggaattttt gggcgcacat
    11881 cgtatgcctt ccggtacgtt cagcgaatcc ggtacagata ccgtggtgga cgtatgggta
    11941 ttgcgtaaac acccggaaac cttcctggag atgatccccg acacggatga ttcaacactc
    12001 aaatcggcaa acgtcctttg ggataccttc ctcaaaggaa aatggtttac aaccgaaggg
    12061 aagcgatttg tttacggcga catggaacgt accagcttcc gcaataccct ggtggtgaaa
    12121 aaggacggtc gtgtatccaa tgagtcgatg aaaaccgcgc tatctcgtcg ctttgatagt
    12181 cgcattaatt gggatttgct cggcgtaact actcaggcat ggcagggcgc gaaagtgggc
    12241 gataaacgcc tagtaggcgg catctggcat gagtttgatg gcctgaaatg ggtcaaggac
    12301 acgacaacca aatcctccgc gcttgatgta agtcgatatg gtgtttcgac attcggtgat
    12361 ctgcaaacaa cgttccaatc aacaaacggc attctggctc tgtcatgggg gcagatatcc
    12421 gcgatcgctt ccgactatcc atcggttatc agtgatgaag tagcggcaat gattcgcttt
    12481 gctgggaaac aacgcgaaaa agaccgtgag cgcattatgc gcggtgcgct gatcgggcaa
    12541 cttatcaata aagcgctgga taagcgtaac cttggtgaaa acgtcgatga tgaactggct
    12601 gatgccgcac gtctgacggc tgccgaagtt gctaaatatg gcccaccaca cgccattaag
    12661 ctaaacgaca ttaccgaagc gggcgcgaag aactggctga cattcaccgg gaacgtcaat
    12721 caggacggtg aagcatccga tctgctgtcc ggacggcttg atgttagcaa aggggctgct
    12781 ggtattgatt tcactcgtcc ggaacaggtt gtgacacacc ttttcagcga tgttgccctt
    12841 aatcctatca atcttgatga cttccgcgag gcgtttaaag gcgaactgcc agaagacgac
    12901 gatcaagcac tggaatacct ggcgaaattc gacgatatcg ctattgatgg atatggttgc
    12961 ctgctaccga tgggccgtgc cactagcggt gatattaaat caaaagtggc actgctggtg
    13021 ggctggcgag acgcgtccac aggagagcgg aaggccaact ttgagcgcca gctttcaaaa
    13081 atcgaagaga aacgcacgtt tacaccgctg aacaaagtca cggtaaacct taacgcacgg
    13141 tggttggatc gccgcctgat aaaagagttc ctggcagagc agggctatga cgagtttaag
    13201 tatacagaac ccgaccttaa ggttgaggac ggcattctgg tatctccgga cgattacgaa
    13261 ggtaaggacg gggtatttac tggctatcaa ctacgaaccg taaacggcaa aaacgggaac
    13321 gagttcaaga aagcgaataa caaagatggc ttcctgaatc agcttgagaa ctacctgaac
    13381 ggtgttaaac cgcgcggaca gaacgctaac gagtacctgg acaaaatcgc ccagcttgag
    13441 acgagtttta acgactggtt aagaactcac ccgcaggcag accagatcgc ccgcgattat
    13501 aacgacgcct ttaacggtta tattccattt acccattcag acgcacccct ggggcttgaa
    13561 ggcatcagcg ggaaacgtat accgctttct taccagaacg aagaagttcg tcgtctgtcg
    13621 gaagatgggc gcgggattat gggattcgga actggtttgg gtaaaacgac gaccgcgctg
    13681 gcactggaag cctataactt tgaaaccggg cgaaccaaac gtacctgtat cgttgttcca
    13741 aaggccgtat accagaactg gtatcacgag gcacagagct tctacagtgc agatgccttc
    13801 gccaatatga tgtttatcgg tctggacgaa gtgcgcgatg gtgacggaaa tattcagact
    13861 gccccagtgc tcgaccagga cggcaccccg cgacttgata acgacggcaa tccgatcacc
    13921 cgtaatgttg ttaaagaatc cgccagtgcc gttatcgctc aacgcatgaa catgatccca
    13981 tcgtcgaact ggcgaacggt catcatgaca aaagagcagt tcgcatctat cccgttgcgc
    14041 gatgaaacca tcggagaaaa ctcacagcag gctgtcttta atgccgccga aatggggcgt
    14101 cttgacctgg cgtcaggcaa gcaccgtgat gcgcagaaga agaacaagat taaggaccag
    14161 gccgcgaata ccggtacagc gaagaagcag aatatcccgt actttgagga tatgaacttc
    14221 gatagcgtaa ttgccgacga agggcacaac taccgcaact cctttggtgc cgggcgtgaa
    14281 gcggggcaac tggcatattt acctaatccg gcggtatcca aaatggcccg tgacatggct
    14341 gtaaaagctg cgtacatgat gaaacgacac aatgggcgcg gcgtcgtaat gctgacagca
    14401 actccgctgg ttaatagccc tattgacgcc ttcaacatgc tgtcaacggt gatccctcag
    14461 cacgagtgga tgcacatggg gattattacc cctgacgact ttgtgcgagt gttcggtaaa
    14521 acagccactg ttcaggtcca gaaaatttct ggcgaagtgg aagagaaaca ggggctggtg
    14581 ggcttccaga accttgatgg tctgcgtggc attttccacc gctggacaac gcttaaaacg
    14641 gcttccgatg ttggtgcaac ggtaaaaatc ccggatatcg tcgaaaacac cttgcagatc
    14701 ccaatgactc cagagcagca agcagcttat gaagagcttc gtaaacgcgc gcaggagtta
    14761 agtcgtggtg atgacgtcac tgtcgatgat gaaggcaata tcactggcgg gaaaccggat
    14821 gatttcattt tctctatcat tcgcgacatg gataaggtcg ctatcgaccc ggatctttac
    14881 gccagcgcta tcacattcca gttccctgta gaacttactg aacaggtgaa gtctatagct
    14941 aataacctac caaaagtggc tggtgggaaa gcggcagacg atgcagaaga agcagaggag
    15001 gatgcgaccg gaggcctggt cagtacccgt accagcaagg ttgtagctac aacatttagt
    15061 gagcatccaa atcacgtcga attgcgtgcc agcattgacc tggaggctga aatactcaag
    15121 gcaattgctg atgcaggcat cgacatgaaa caggtgtcac acccgatccc gcctaagtat
    15181 gccgcactta tcgagaacct tcgagaaggg cttaagaacg gtaagcaaat tatctttatc
    15241 gacgagaaag cccagcacca gaaactacgt cgcatcattg ccagtgcgtt gcaaatgcca
    15301 gagcaggaga taggcattat caatgctacg actgttagcc aggctggtgg tgttaagctg
    15361 aaaaaggtca aaaaaccgac cgaaccgacg ccaaacaaag acggtgaata caaagaaggc
    15421 gcatgggaaa cctactacag taaactggcc caatatgagg attatctctc tgctaaaaat
    15481 gatgctggcc ttgaggggat ggaaggtatt gctgccgact ataacgaagg gcgcacgcgg
    15541 atcattatct gtaacaagaa agccgaagta ggtatcaacc tgcatattgg aacgaccgat
    15601 attcaccacc tgacactacc ctggactcct gccagtatcg accaacgaaa tgggcgcggt
    15661 gcgcgcgtag gttctccgca agagaaggtt aatgttcact attattgtgg caaaggcaca
    15721 ttcgatgact tccgtctgga tacgcttaaa cgtaagaaag actggatcaa gatggtgatg
    15781 acctccgata tgtcagagat cgccaatggt gatgcggatg acgctgatga acgcgccatt
    15841 atgctggcgg caaatccgga agaacgacgc gcaattatgg ctcgtcagtc gaaagaacgt
    15901 gaggaacgcc tgcgacttaa agcgcaacgc gaagccaata atgctctcga taactacctg
    15961 aaagcggcga atgctgccgg gaaggatatc ggcatgatgg aggcagaact gaaaagcgcg
    16021 atggagcagg cgcaacgata ccagagtgat ctggatggac tgattaagag cggcaccaac
    16081 aaaaacggac agaaatatac gctttcagca ctaagcaaag ctaaaaaaag agtgcgtgaa
    16141 ttgcgatttg caatcaccag ggcgaaggac gcggatactg ttatgaaacg ctctcgtggt
    16201 gatgtggagc gtgctattaa gtctggtgtg ctggatatca gtatcgatgt tattcagaac
    16261 ccgcaggagt atatgcgcac tggcaagaac atcctgttgc ataaaggtag ttactaccgt
    16321 gcagtcattg atgctgaaca cgagacaacg gcggttgtgc gcgtgacaca aattgagggc
    16381 gaaaaagcgc aatacacctg ccgcgtcgcc tggctggata gagcaagtcg ctactctcgt
    16441 tctcctggct caaccatgac attaccgttt gactcagtgg ttgaagcggt aacatttgaa
    16501 gagggggtgg ctgaatcacg agaaatagcg gcacgtggtg ttcaaagcca ccagctttca
    16561 acctctcttt ctcgcgatca attctatgac gctattcgta gcggtgttat gcgggtaact
    16621 gggaaagatc gtggcggttg gcaaggagca ccggatgtaa actattgggc ttatcgtaac
    16681 gccaatggtc agattcaaat ggcctttacc tatggcggga tgattggtca aaaaattagc
    16741 gcatctggaa gtgacgagat agcacctgaa caatggattt atcctgatgt aaacgacgaa
    16801 gcattaaaac gcgaagtcgc gaaaatccag acaggtgata acccacttcg tatttcagag
    16861 gcagaaggtt ttctccgtgc attgtttggc ttcgattatg agcaaggtat ggaggcatgg
    16921 ggcgctcagg ccactatgga tgatgtggtc aaggcgttcg atggctggat ggcagggcgc
    16981 gatctctccg ggcgttcact tgaaaaagtg actcaggaag atgtccgcag cttgttccgc
    17041 aagccaaatg gagaaggcct ggaaacgtac tggcgaggcg tgctttcttt ccgcgatttc
    17101 caggggcgca tgaaagcctt caccaatggt tcggagattt cgcagatgtt cgaacaactg
    17161 cgaacgtcca aaatcacaac caaaattgag aaaacacgca tggcgctaaa tgcatggcgt
    17221 gatgcgctgt ttgatcagta caaaatgcaa agtgatgcag atggttgggc gtcacttgct
    17281 tcgttggcag atcagtcagt ttacagtgcg caactaagca tttctaatgc cgataatccg
    17341 cttgaaacat ctgcaacagt ctggattaaa gcgggggcgt taattcatgc atttgaggat
    17401 aaaagcatta cccgtgatga tttcgcagat gcagatagct tgaatcgtct tgtcgggatg
    17461 gctatacgcc atgctaacgc cttaaaagcg ggcgggtatg tggaagagtt gcctgatgct
    17521 ctgaaatctg caacgtggga tgactttgtg tcgcttaaaa atagtggcgc tacggagtca
    17581 gagattgctg aacgtcttgc ggcggctaag actgaacggg aagaacaggc tgatgctgct
    17641 gagaacaagg ctgacgaggc agcaaatgat gattacatta tcgtggtaaa cgataagcct
    17701 attcgcgcca aagcacgtgt ccgcggtcgc tggtggagcg taagtgaaga cgctggtgct
    17761 gtttatctca ttgcagatat gcccggatct tcatctattc gcaacgcaaa agatgccatc
    17821 aagaaaatcg gtgggcgttt ctggaacttc gaagttaacc cggttgccga tgtggacttt
    17881 gaccgtccgg cctggatggt atcgacgcgc tattcaattg atgaagtgcg taaaatcatt
    17941 gaagcggcgg cttaacagat atggagcggc ctcgaaagag gccgaattaa aatgacaaca
    18001 ttgatcgaca cagtaaaacc aacggaatcc taccttgagg acattttgcc gcaggcgctc
    18061 aatggcagga gtgaagaggc atttctggat gcatatctga atggcatggt ggaaaggctg
    18121 aaacgagatc cacagcttta tcatctttat gggccgtggt ggcctgcgct taaaacgtta
    18181 ttgctggagc gcggcgacat atcatttgga caagttgtag atagtgatgt ggcagagatc
    18241 tacaaaatga gtcgcccggc gcttacggtt ttggctgggc atctgtattc gagcgatcgc
    18301 ctggaaaatg acgcggtata caatcctgtt catgctctgg aagtcgcacc ttatgccgat
    18361 gatacagagc catacgtgta cacaagttac gacgagtcta tagagaaata tcgaatcatg
    18421 gggtagccat gcgaaagaat cgtaggttta cggtcgaaga cctaaaggaa tattccatat
    18481 cgaaggggta tgtccttgag ttccaccgat acaagaaggt ttttacgctg cgaaaagcag
    18541 aaaaccctgc gaactggagc tggatatact ttcctcacac cgatgataag ttggtagaac
    18601 ttgttgatga tttaacctat gaaggttggt tgatcgcaat cgacaagaca atcatagaat
    18661 tatccgaaca ggataaaata taacccttta aaaataacag gttgggtaaa tgccatgcga
    18721 tttaccctcc ttgttgatta aggtcacgct ttaccttaaa aggacatccg acctgttgca
    18781 tatagataat ccttgagtag tatctctctt attctacaaa cctaacgttg ttgttattat
    18841 tttgcatatt tactatcaat gggtgtgtta gaaaatagag cgaaagagaa ggcgtcgcag
    18901 tgaatattct gaaacatccc cacatgccgc agctcttaga gaactggaga aaaacccaga
    18961 tctaatcaaa aggaaccatg agcattacga gcaggttcgc gtcctggact ggatgtatcg
    19021 caatatgcgt tatgtgtatg agcatactca cgccactcca aacggtggcc tgcgaggcat
    19081 aaggacggca ataaaaatgg tggcagaggg ccaaaagaag ggatatcccg atctttctat
    19141 agaccttgct tgcgggggat accacggaat gcgcattgag atgaaacatg ggagaaatcg
    19201 cctgactccc gaacaactcg tctggatgac tcgcctcaca gaagccggtt actactgttt
    19261 tgaggcgcgc agtgccgctg aagccataaa agctatcacg gagtatgttt gtcttgatta
    19321 aacggagaaa tcgttttgcg tttacttaac gtggcagaac ttatccctgc aggaactaat
    19381 gctatcgctc gtcgtcgctc atggcagcag gctctatgta ttgagatctc gaaacgtatc
    19441 aatggaagtt ttgaagctcg caatattgtt actggcgata aggttcacat aaccccggaa
    19501 agtgcaagcg ctgatgattg ggagtttgta cattgagcaa aaaatattat gtgagtctgg
    19561 cgttcgctga cgatgcgggg aggacgcgga gtatcacgtt gagcacaccg gttaaggctg
    19621 tgacagcgcc gttaatccgt gaagctctca gagagctgga attgggtgaa aactcggcac
    19681 tgttgtcggt tagttggtta gggaaaatgt cagagaaaca atacgtggat ggagttaccc
    19741 caataacagt aatgaggcta ctttcattgc tgcaatgggc catcgtacct gtatttatct
    19801 cgtatcttat ttatcaggct gcaacacagt aattgcataa aaacccgcca attggcgggt
    19861 tttttgttta ttgcatcttc cgctaccgtc atcgaactaa taaaatatgt tagaaaacta
    19921 atttaacaag gtaacgggaa cagcaatgag tcaacacact gatacttcta atctggaaat
    19981 tatctcgaca gcaattgaaa cgctgcgcac acaaatcgct cttatacaga aacggaatcc
    20041 gggtgatgac ctgtcaaagc gactgcacga gagtgtcatt gccactacag ataacctggt
    20101 ggcggaaatc aacaagctgc ttgatgatgg ggcagttgat tacaataagc tggtggatca
    20161 gtttgaggaa taccaacaag ccgttaatga tggcttgatc cgtttttcac aagtcacagg
    20221 cgtatctgcg acagttgaaa gcctgggtga tgcagttaat caatttgccg tgaatatgcg
    20281 taatgagatc ggcaatctgg aagcgcgtct tgaacaggca aatacactgc gtaaatctgc
    20341 cgaagccgat ttgaaccgtt acaaaaaaga ctatccgccc agcctgacaa agcggctgga
    20401 tgttgcggag aaggataacc gcacgctgaa aagagagcgc cgggaactaa aagagcgttt
    20461 aaccaagctc aatcagcaat gcattgatta tcaaggcgaa ggcgtcacac tgcgtaaaaa
    20521 gttggctaca gcacagagca ttatcgaaac attaaagcga gagtgctcac agcttggtca
    20581 cgatctgaac cgtgcttgtg gcatgggaca aagaccagag acatttccac ttatgtatga
    20641 tgggcgggat gccattgcct acatccatga gtatccgcac ggcctggttg cggaaactgg
    20701 gcagcgtggc gaagcactgc tgacggctaa ttaccatcaa caaatcagaa ccaatcgcct
    20761 gttaacgatg gatgtgatcc catcggtatg gggaacgccg ctttactatc gcctgccggg
    20821 cttcgaaaag gactggaata ccgacataga cgaatgtctc gcagataaaa ttatggcgta
    20881 cctggagacg gattttccgc gcttatatcg ccggattatg gactctaagg atgcgccaat
    20941 cgatgaatta aaaatgcgtc cggagacgct ggaggcaatc aagcagacgg agtttgatac
    21001 tgtattcagt gtggcctgca ttccaagctg tttccatgaa agcattccgt tcatgcaagg
    21061 agatcgtcgt caggaaatca ttgacgcttg ccgggtatgg gcgaacgaat gggataagaa
    21121 gaatgggggt gtagaagatc tttacgggaa ataatgtgtt agaaatctaa aattacgatt
    21181 cgaatgtatt gattgtgaag gtaacttaac atagaatttt ccttgctggt gggcggcacc
    21241 cacgtttcgc ttgctgggct tgatagtcac ttattggtta ttgttcgccc accagcattt
    21301 aacaaattaa agcgttagaa aagtatagca atgcaagagc acgctttggc accgtgcagg
    21361 ccaaagttca gcaggtttag tgcggaaagt ggtttcagag agagcacttt gcggagtaaa
    21421 cgacagtcca tgttgagcca cggaaggtct ttttaaggtg ttcattgatg atggtccggc
    21481 agagagtaac ggtacatgct gcatctaatc atcaatgcac atcttcaaca ttaaggagac
    21541 tcagggcaaa aggtaaccgc cctatgcctc cttaatgttg cggtaatgca cctatgcgga
    21601 tgtggccttg cattgtgtct tgaaagaatg gggtaaggcc ggggatatcc ccagggggag
    21661 gcacccccgc tgcaacgaac agcctcagac ggcagtcgag tgatgggaac gtgcaaagac
    21721 tttaaacctc gttatggctg gcgtggcaaa cgcgacaacc gagacagagc ggtgacgggc
    21781 ggagagagtg ccgcatcaag aaagaagccg acttgtgatc cctttcagcg ggcatttgaa
    21841 agtgaatcca gttcaggcag tcggcttctt tcttgtgtga tacgtagcgt atttgcccat
    21901 cttcgggtgg gcttttttag ggttttcgtc atggttagcg actttgcggc ggtttagaaa
    21961 ccgaccatta aagtaaatgc aaacgatgat ctgatgatgg tagcggccta agaagcctga
    22021 cgccacgggg tatgagtcgt cccccgtcaa aaaatcgacc gcagagtgtc cccgtctgtg
    22081 tattagggaa cggggaggca taacaggcaa gggcgctggt gtgattaacc agatgaatga
    22141 gaagggtcag gtctttctgg tcagcgccct tacctgttac gtccctcccg cgtaacagcc
    22201 attggttaat ggctttctgg gataatgtct tgcaacgggt atatcccgtc atgctgaagg
    22261 cgctaatcac gctggaagcc agggttatgc atcccctgtt accgaattgc agccagggcg
    22321 cggtgcgccg aaaagcatac ggaggtggaa gccctcgccg gagacgtacc cggcaagtga
    22381 tggtgtagct cagcggtaga gcagttggct gttaaccaac tggtcggtgg ttcgaatcca
    22441 cccaccatcg ccaatttagg ggagttagtc cgtaggggca gcggggtaga ctgtaaatct
    22501 actgtcattg cgactcgggt ggttcgactc catcactccc caccaaattg ccggtttagc
    22561 tcagttggta gagcgcctgc cttgtaagca ggatgtcagc ggttcgagtc cgttaaccgg
    22621 caccaacaca acaggtaagg gtattttgcg acgtcggaga tcgccgagct tggcagaggg
    22681 ttcgaatccc tacgaagtac ccttaccgtt gtgatgaagt gcagctcttt gaagcaacca
    22741 gaagataagc atctggcttc acaacataaa ccgcaggaac gaccaataaa cggtagtccg
    22801 tatggagaac accccgttga ggaagaagcc tggcaggaac cgtaaccggc actacaacgt
    22861 tgagaacatt ggcgtaacgg ggtcatatcc caatctatga ataaaggtcg cgaggcagcg
    22921 tgacaaccag tgttctcaac attgtggtga atgcacaggc tgatgtgccg caactacagt
    22981 agtgcgcgct ttgcggggct tgctacaacc ctgtgtcgga gttcagcacc gaccatcaca
    23041 gtttgattct ctggcatgag cataacgctg aaataagtcc agtatggtgc ggcccgatca
    23101 cccgccgtta gctccacgaa acggagcgca caacaggtaa gagcattggg ccgataatcg
    23161 tgaagagtcg gcgagcctgg ttagccaatg ctctttccgt tgtggttgat gcgcagactg
    23221 atgcgcggaa agaaatgccc cagcgacaca cgaggcgagt tcacacactg caatgcagtc
    23281 agtagccggg gtcgctaagc cggagatcag taccggtaac cacaacaatt ggagagtagg
    23341 gagcatggtg ctcaagcggt cttgaaaacc gtcccattgc gcaagcgatg atggttcgat
    23401 tccattactc tccgccagcg caacgttaag agtatttggc taattaagcg aatgccggaa
    23461 gcagaaccgg atcaccaaat gcgtacaggc gtcatcgccg cccagccagc caagtgctct
    23521 taccattgcg gtgaatgcgg ctaagcgcac gcggaacagt taaaacaatc ctccgtagtg
    23581 gttaaatcaa acatcccggc gttaattgtt aactggttaa cgtcacctgg aggcaccagg
    23641 caccgcagct tttttattcg ttgaataatg gagtgagagg atgcagaaca atccgaacaa
    23701 atgtcgaacg ctatgggtgc ggctatatat ttatgccgtc ctctgtttga ttgtgtcact
    23761 ggttctgtat gtttggcttt tgccaaatat gatctcatct gacagcacaa tacttgtatt
    23821 gttgggagtc cttctcgcgc tcatttaccc ggcttttgca gtagtctttt ttcgtgaaaa
    23881 aaccaggaaa ttgattaatg aaaaaaacgt tgattagtgc agcgattatt tttggttctt
    23941 tatgtctgac cggatgcgac cgggtagaac caggcaatgt agggatcaaa gtaaataagc
    24001 tgggggatga taaaggtgtc ggcgaagtag ttggcgttgg tcgctactgg acaggctgga
    24061 acacagaagt ttatatcttc ccgaccttta agcaaatgaa gacctatgat gatccgttca
    24121 gcttccaaat gagcgacggg acaacaattg gttaccacat tggtgtagct tacaaggttg
    24181 atccggcaaa agtcacaact gtatttcaga cctaccgcaa aggcgtggat gacattaccg
    24241 acaccgacct gcgccagaag attgccgacg cacttaaccg actggccagc aaaatgacca
    24301 ccgacaaatt tatcgacggt ggcaagtctg aactactgga tgcagctctt aaagacatcc
    24361 aggaagaaat gacgcccatc ggtattcagg taatgagcct ctcatatgtg ggcaagccgg
    24421 agtacccgcc aaccgttatc gacagcatta atgccaaagt cacggcgaac cagaaaactc
    24481 tgcaacgcga gcaggaagtc aagcaacgcg aagcagaagc caacatgctg cgcgcagaag
    24541 ctgccggaca ggccgatgcc attcgcacaa aagcccaggc tgaagctgat gccattcgtt
    24601 tacgcggtga agctctgcgc cagaaccccg gcgttatgga gttggaagcg atcaacaaat
    24661 ggaacggaac gctaccgcag tatatgacca gtaataccgc tgtaccgttt gttccggtga
    24721 aataaccaaa tagagaacat gatggaattt tggacgtttg ccgacaagaa cccttggtgg
    24781 acatttttat ttctgctggt ggttgcatat ggggtcgagc aagcagcaaa agccttacgc
    24841 ggccctggca aaaagcgtaa gcggaattaa cagtaatctg gccctttagc tcagtggtta
    24901 gagctggcga ctcataatcg cacggtcacc ggttcaagtc cggtaggggc caccatattt
    24961 ggttgtaaca cggcgtatgg cacatgcgtc gttagcggtc tggtgacgtt aaaggggtta
    25021 ccttttcccc tagctcaggc aacaaaccag gtagccggaa tgtgcaagcc ccgttcatat
    25081 cgtcggaccg aggactcacc accctggcga ttcggtgtga cagccggaag agtacggcgc
    25141 acaacaggaa aaggcgttta attaacgcga gcgccgtttc cgttgtggta attgcggcta
    25201 tgcgctcgtg acgaagccat tttcattcac tacgttctga taagtgacgt cgccaggagt
    25261 ggctaacctg gcaggtggag gcaccaccgc cacaactcct ttcaattcat catatagccg
    25321 ctcaggctgt aacccttaat taatacgaag tttcgacatc tctatcggtt taaccgagag
    25381 atggtcgcgt gtgtaaaaag gagacgttat gttaatggcg aacgataacg catatgcaga
    25441 agaagatctc attttgagtg acttcatagg gaagcgggaa cgctggaccc aaaagcgaga
    25501 ggaactttac gcctcattag ttcgcaaagg cgtcaatatc gaaaccgcac agagcggcga
    25561 tatgacagtg gtaagcgttg gattgcacgg tgtaagcgtg tcagcaatca atcatgagcc
    25621 atacgttgcg ttatccgagt caatggtaag gttagttaag ttcctgaagt acacagaagc
    25681 gaataatgta atcattggca aaaagaatat ccctttttcc tcggcttttt actggatgat
    25741 gaaagggctt gatgcccggc ggacatcatg gcctaaaggg agctatatat cgatgttccg
    25801 tggcagcatt ggcagcaaag aaaagctctt cgaattcttg ccggaagagg cattcgatat
    25861 tgtggaaggg tgcgatgtga tggtgatgcc tcgcctggta atgatgaatg gtgatttaca
    25921 ggcgcagaca gactggtttg caactggtgt ggatattatc gcgactgact gggaggcttt
    25981 ctgatgtacg ggactaccaa attaagcact caacaggcca ccaatatgga gtggtctatc
    26041 gctaactgta agatcgtgtc atgcaatgcc acgcgtactg atgtgggcca taaaagcact
    26101 ataacaggct atctggtaat gaccaccagg gggatgaggg ttagctttcg ctggaaagca
    26161 gagcaagtaa cgccattcag agagccatca atgccgctgc aggttgagca ggattatcta
    26221 tgtttaccag aaatagatgg cggaatgctg gtggagtctg atggtagtaa ggctgatttt
    26281 gaagccaatt tgaagctggc acaggccgta atcaatagtt gtcgcggaaa gtggatcgca
    26341 aaagtgaaac atgttgttcg tttgtctcgc tggtagcgtt agatatttag tattaatttt
    26401 ctaatacaat catctttttg ccagtctata caattttaac attctcatta tcatcccctc
    26461 ggtcatttcc tgcccggggg attaatggaa caaatcagca gcgcagaaat tgcggatatc
    26521 atgattaggg ctgactgcta tctgacagtc actgaaatta ccattctcgc caaagaaaaa
    26581 taccctcatc tccatgtctc acgagtaagt gtaactaaca tcattcgtca ttttgttcgt
    26641 tcaagcaggg caatctgtga gcttgatgat cgcgtttacc cacgaaaata ctggttgcat
    26701 ggcctgaacg gatatcagtt caaggtgcgt gggcgaaccc ctgaatacgg cagtttactg
    26761 gttaaaaact gtagcaggaa atcagtcgaa caggcgcgaa aagagcaacg agagctggtg
    26821 gacatggcga ataagctgtg gaatgccgcc gttaaaaagc gtggtgttgc tttatgagtc
    26881 tggttaattg ggaggagcac cgcgcccgct ttatagcgtt gcgtgaagaa aaagggatca
    26941 ctgtaaagga atactgtgag gagcatggcc ttagcttcaa tacagccaga aagcatctca
    27001 atatgaaaaa aaatgaggtg cggtcgcagg taaagacaac aaaaaaaagc accaaatcac
    27061 ctgaaaaaaa acgggcagga agagcaacaa aaaagcagga agagggtgca gaaaaaacaa
    27121 aatttacaga aattgacaat tcgcagggcg caggttcttt ccgaagtatg ccgagaaaga
    27181 aaacttcgga tcatgaaaat aattcaaata ataacaatga gataaaagaa tccgtcagga
    27241 gatcattgcg cgaaaaaatg gcgaaaatga tccccgaaga agataaaagc gccccaaaaa
    27301 ttgagggatc ttctgcaatg atccctgacg gtgctgtgca gcgtgccaca ttgccaacta
    27361 cgaatgttgc tcgcgatatg atgaagaacg gcgcagaaga acacctgcgg ttagcaattc
    27421 aaatggcgca agagcgcgca cttcagtatc aaagcattgt tgatcaggag gccgaacgtc
    27481 tacaggcgga aatagacacc ctgggcgata aagagccaga aggaatgcac cctgggcagc
    27541 gccttctcgg tctaatcagt gacgcggcct actacatgaa tgattttatc agtaggttgg
    27601 cagctatata tcagtccgaa caaaagctgc gacaggggga tgaaaagctt cgtcagagcg
    27661 ctcgccagca agcattcaaa gaggctgagg ccagagaaaa actcgaatta gcacgcctac
    27721 aggccgaaca gcgtggcaaa gagattgaat atcgcattgg cgctgacgca cgcgcagcca
    27781 gagttatcgc cgccgctata cgtatgcgtg agcgcgagga actggatgat atcggtgttg
    27841 ctgaatacat cgagcgccag ggggtcagcg tcccggctat cctcgcggcg cgcgcggcga
    27901 aagctatcac tcttctcgaa ccacctgttt ctgacgtcaa tgatgttgat gatgaacagc
    27961 tggacaaaga ggcaagggag tttgcaacgc ttcaggctaa ccatccgcaa tggctggcag
    28021 agcgccgggc ggatgtggca accattgttg aagaattggg gtgcggagat tatgaccgca
    28081 atggcgagcg taaagctgga gaattcgagg ccaacgacga agagctggat attgaccctt
    28141 cggcaacggc tgaaatttac ggtgattatg atgcatctga tgcagggtat gacgccggag
    28201 atgatgatat cgcgatagaa ccgccggagg atgattaatg gctggcgggc gcaaggttaa
    28261 atgcgtcacc agagatccac gctggcgcga tatggtaatc aaataccgct acaactttac
    28321 ccaggctgtt gtcgatattt ttgggatgtt cccctccccg cagcagcagc agatcattca
    28381 gtccgtacag gaaacgggga gtaggactac cgtcacgtcc ggacacggaa cggggaagtc
    28441 gtcccttacc gcgatgttgc tgttgatttt tatgatcctg ttccccgatg cccgtgtcat
    28501 cattgttgct aacaagatag gccaggtaaa aacaggcgta ttcaaatacg ttaaacagta
    28561 ttgggcgaat gcagttaagc gtcatgggtg gttgcagacc tattttgtcc tctctgacac
    28621 catgttttat gagcgctccc gtaaggggat ttgggaagtt ctctgcaaag gttatcgact
    28681 cggcaacgaa gaagcgctgg cgggggaaca cgcggcacat ttgcttctaa ttctggatga
    28741 agcatctggt atatctgata aggcgatcgg cgtaatgacc ggtgctctta ctgaagaaga
    28801 taaccggatg ttgatgctat cccagccaac aaggccaagt ggttatttct acgattcgca
    28861 ccattctcga gcaaaaaccc cagacaaccc gaaagggatc tggacagcaa ttgttcttaa
    28921 ctcggaagaa tcaccgttcg tcactccaca attcatcaag gagaagcttc tggaatatgg
    28981 cgggcgcgat tctattgagt acatggtcaa ggtgcttggt cagttcccac gagaaattaa
    29041 cggctatttg cttggtcgtg atgaatgcga tcgctctgct cgccgcaagg tgttgttgga
    29101 gaaaaactgg ggatgggtgg caacggctga cgttggtaat gggcgagata agtcggtcct
    29161 caacatctgc aaggtatcag gtcacaggga taagcggcgc gttgttaact ttaaggtaat
    29221 ggagatgcct ggcactatgg acccattggc ctttgctgac ttcatatata acgaatgtac
    29281 gccggaaaaa tacccgaata taacaatcgc ggttgatgct gatggttttg gttctgatac
    29341 gtgtgcgcag ttggtgcgtc gtggtgcgaa cccggtacgt attcggtggg gaaaacccat
    29401 gtttgcgaac aaagacaggg agcgatttgt taatcagcgt gcctacgcaa atatcatggc
    29461 gcgtgatgcc ataaagtcag ggcgtatgcg gatagacagt gaccctaaaa cggcagagca
    29521 agcgtcaaaa atcccctttt taatgaatga agaagggaaa atggcgatga tgcgcaaaga
    29581 gcacatgcgg caaaagctaa acatcaaatc gcctgaccga tgggatacct actgcttcac
    29641 tatgctggtg gactatgtgc ctgctaatga agatattggt gccgaaatgg caactttccg
    29701 agatcaggtt ctggcggata tcgaaatgcc ggatctggat atataaagcc ccgcgatgcg
    29761 gggccggttg tgattaccag agcttcgcgg cgtactgcgt ttgggggaat gtgtatccgt
    29821 aacgttcttc gattgctttg ccgtattttt tacgagcttc attcagttta atgccggtca
    29881 ggtcgtctcc agaggccatc attacgtcta acaattccag atccatttta tccagccttg
    29941 ccattgcttc acgtgatgta cgtatcttac gctcaatgat atggcgaata gccggatcgg
    30001 catcctccag catcttagcg gatttctgct taaggctttc attgcgaata tcgatgttcc
    30061 ttaagatata cgacttcgca acgtagcttg gctggtgttt ttccaggtca cgaacacgat
    30121 ataagttaaa atcgccgaga aggctgcaag gtaccgattc gacgccagaa taaattgtaa
    30181 tgctccggcg ttcaccaaca ttattgacaa cggggatcgt tgtcttcggt agtcttgcga
    30241 aaaccaatgc gccatgtgcg ccaactctaa agcgagaaag ggcattactg ccttcgatgt
    30301 ttgttgtcag cacaacatct gtatatgcct ctttacgcgc ccggtagatc tcgccttcag
    30361 gctcagcttc aaagaagcgc tgttcttctt caagagcacc tacgatttga gaaaaaaccc
    30421 tatttttcag gcttttcttt atgattttgt cgtatttttt tagcaattgt tcacgtgtaa
    30481 atgcacgtgt tgttgtagcg ctttgcacaa cgacatgcgt gctttgattg gtgtcctcaa
    30541 tgatgaatgg gatcggagaa tatacggact caacaatgtt cgacgctttg ccgtcggcat
    30601 cgcggcacat gtcaacgaag gctttccatt cagcaagaca tttttcagct tcttctaata
    30661 tgttttggtc atgcgttaat gcaacatggc ggaattcctc accagcataa ctggtatcat
    30721 ggaaaacaga gtttgcgacc accagctctt tccaccgttc tgcggtttgc aggaaccgtt
    30781 tgtactcttt ctcgatcgac ttcatactca tcattcctac gcgatttagt tttctaacat
    30841 aaaattacta tgacaaattt atgtcgaata acagagcgag taaaggatcg gactatgatt
    30901 tttttgtcat caacgttaga aaaccaaccg ttgaaaccac acttcgcaaa ttaaagacca
    30961 tgaaggcaac gttttactgt ccatcatcaa tctatgatgg attacaaaaa aatcggcatt
    31021 ttataacttt aaataatggg gattatttaa agttagacta taatcatgtg tagatgaaca
    31081 gtattgacat cactgaaaat acgtgcctcg aatcaaaggc ttgtattttg aaaattaact
    31141 tatagctacc ctgtcatttt tatatttttc gcgtagcgtc atctgtatat agaggtgaaa
    31201 tagttccatg agtgagttga ttatctctgg cagttccggt ggttttctgc gcaacattgg
    31261 caaagagtac caggaagccg cagaaaactt tatgcggttc atgaatgacc agggggctta
    31321 tgcgccgaat actctgcgcg acctccggtt ggtgtttcat tcctgggcgc gctggtgtca
    31381 cgctcgccag cttgcctggt ttccgatctc accagaaatg gcccgcgagt attttcttca
    31441 gttgcatgat gccgatctgg cttcgaccac cattgataag cactatgcca tgcttaacat
    31501 gctgctttcg caatgtggcc tcccgccgct gtcggacgat aaaagtgtgt ctctggccat
    31561 gcggcgcatc cggcgtgaag cggcaacgga aaaaggcgag cgaacaggcc aggctatacc
    31621 gttgcggtgg gacgacctga aactgctcga cgtcctgctg tccaggtcag aacggctggt
    31681 ggaccagcgc aaccgggctt ttctctttgt tgcatacaat acgctgatgc gtatgtcgga
    31741 aatctcgcgt attcgtgtag gagatctgga ccaaacaggt gacactgtca cgctacatat
    31801 ttcacacacg aagacaataa cgaccgccgc cgggcttgat aaggtgcttt cccgtcgcac
    31861 tactgctgtg ctgaatgact ggctggatgt ttccgggctt cgtgaacatc ctgacgcggt
    31921 gctgttcccg ccgatacacc gtagcaataa ggccaggatt acgacaacgc cccttactgc
    31981 acctgcaatg gagaaaatat tcagcgacgc ctgggtgttg ctgaataaaa gggatgccgc
    32041 gccaaacaaa gggagatacc ggacgtggac cgggcatagt gctcgtgtcg gggccgctat
    32101 cgatatggct gaaaagcagg tgtctatggt ggagatcatg caggaaggaa cctggaagaa
    32161 accggaaaca cttatgcggt atctgcgccg tggcggtgtg tctgtggggg ctaatagccg
    32221 cttgatggat tcgtaaatta aatttctaac acatcttttt agattgttat ctttgctgta
    32281 aagtaccctc gcctattttg gagggtgctt tgcagagtca aatcacggaa tcaagacgct
    32341 tcacggtgga tggttacacc gtaaccttcg ccgctggtgt taagaacagt catgctgtcg
    32401 ttgtattcgg catcatgacc gggcttgagg ccgtccctgt gtttaccctc gaaaaaaact
    32461 ggcgaaatct ggaggaagca gaaagctacg tccggaaagt aaccatcatt gccgccgaaa
    32521 aactcctggc ccactatcag gaaaattatc gcactatggt cgagaagatt aatctcgctt
    32581 tcactcgccc cactaccggc tttattgcac cggatactaa tgcaggtcga tatccggcaa
    32641 gggggcgtcg gtgaaaactt attacccatc tctgaattgt tacccgtctt cctatccagg
    32701 attgtccggg aagctgttag ctctattagc taaagcacct acggaatggt ttaagccagt
    32761 cgttatcaat ccacacttcc tggattacgg atctgttcgc actcgtaacg aactcaaaaa
    32821 cctactggcg catggattca ttcgacaccg tgccggtaaa ggctatcagt tatcgattgc
    32881 gccagaagag gccgtgcggc tattcgcata tcgtgacagc cagacaaaag cccgggtaat
    32941 agctcaaata cttcataacg gcagcaccta tgcccgccag tttggcggcg acacgtcgca
    33001 gttcttgcgg gcggtgcgat cgctggagga gcaaggagtt attgaaagtt cctgtattcc
    33061 tgttcctacc gttccacata tcaaacgtcg ggtttacacc ttcacgcaga gagctaaaaa
    33121 acaatgattt acgtaaaagt gaaacgccta catccggcag caaaacttcc agcttatgcc
    33181 acctccggat cggcggcgat ggattttgag gctgttgaaa taaaaccatg tgtcgatagc
    33241 aatggtgcaa tttcctcaag ctggtgggtg tacaccggac tggctatgga gatcccgccc
    33301 ggctggtgtc tgaaactcta tccgcgttcc ggattgggct gcaaaaaaca tactcgcctg
    33361 gctaactgcg taggaattat cgactccgat tatcgcggtg aaatcatggc aaaactgatt
    33421 acagatcccg gtggtgaagg cgtttgcctc aagccaggaa tggcggtcat gcaaggcatt
    33481 tttgagcgtg ttgaaccggt atcgctggta gaagttgatg aactcgatga aacagagcga
    33541 ggcgctggtg gctttggtag cacatcggaa gaccgcgttg aaatcaagcg tacaccgctt
    33601 cagtacgcaa agttttaagt agaggaagga ttatggtcag cacttttcag gtgttcccca
    33661 aatacaaatg ccacagcatc attcgggcat cgaaaatcaa agatattgtt attgtcggca
    33721 aagcttatgc gcccattttt ggcagtatcg aagtcgttga gccaaaagga atgacgcttc
    33781 gtgttgatgg tgtgttcttg gaaaaattcc gaccgcaaat aggaggatat ctcgtcactg
    33841 ctgaaaatgg ctatctggtc tatgtgccag agaaagcatt tgaaaaatgc tatagccggg
    33901 ttaacgacgc ctccgaaaat ggggtatcgc tcagcattga aggtcacaat ggggtgacat
    33961 tcattacagc cagggacgta actatttctg ctggcggtat cgctactcta caagaagaaa
    34021 tcgaccttga agcagccgac ttttctgacg cgctgatgtg gctgaaggat ggcaagaaag
    34081 ttgcccggcg cgggtggaac ggcgaaaacc aattctgctg gctggttcct gaaggacagt
    34141 acccggcacg aatggaagcc attaagggat atttccccgg cgatctcgtt ccgtatggtg
    34201 cttatttcgc cttaaaaaat gcacaaggtg tagttgttcc gtggttgcct tctgtaggcg
    34261 acttactggc atgtgactgg tttgtagtgg agtgatttaa cgtggaaaat actaaagcaa
    34321 ttcaataccg cttgcgtaat ggcctgctgg tggctgttaa cgccgacatg tcactgccgt
    34381 ttgacacgat tgaacgtact atcatgacgt atctcggctt taatgaagag ctgaatgaag
    34441 agcatggcgt agctatatgg agtgatgcag aaagcggcgt tcatcgttac atcaccgcca
    34501 ggggcaaaga ctactctctg gaagaactgt ttacccttgc acagtctttc gaatgtgtag
    34561 cattagacat gtttaatgat cctgctatag cgcaacgact tatacgcgag cttgggctat
    34621 ccgttacacc aattatcttt aagaatggca gcctgactgg cacatggcgc gtagaacgca
    34681 tctcaaacta ccttccatat aaccgacagt taaatggagt aatctccggc gttaatcagc
    34741 ccgtagcgtg tgaaaatgta aacctggttg ccgctgtttt ggcaaccgca tgtcgtgtta
    34801 tcggtttggc taaacaggca tttattcatt tcccaaatgg tgcagaaggt agcgcagaga
    34861 ttatcgcgtg tgatttcgag tttacctgga tgctacgcga atatctggac caaacagtgt
    34921 tccgcgccga agaactggat atgtatatca cgtcaatgat tcctgatgat gtgcgtgctg
    34981 aagcaatcgc cacagcaagg gcaaaatgcc gtgcggcgat tgcagagcag gccaaagaag
    35041 aaccgtccga tgaagccgtg gctgatgttg aagcagaggt agcggaggat gctgacgcct
    35101 aaaaacatca ccgaattagc tggctgacag cagaaaaaat cattgagtga tagtaggaag
    35161 cccgcctggt tgagcgggct tcttttcaaa tattggaacc aaataattca cgtagttttt
    35221 ccgctactga agatacgggg atttcaccag ttgcggcccc caccgcaagt tccaccagca
    35281 cgggagaatc atatacctgt ataccgttac ggcgtagaaa tagcagcgca ctgtttagcg
    35341 cggtacgctt attggcatca ttgaatatat gccctctcgc cgtagccacc aggtaggtgg
    35401 cggagacttc gaaaaggtcg gtgatctctt cgtaggcaac tctggcctga actctcccga
    35461 taatggcctc tgccctaccc ggatctgaca ttcccggcag gccgccgtag cggcttatat
    35521 tcgcatcatg aagcgcaata agttcttccg gtgatatatg cctcattatc ggttaaccag
    35581 ttccttgttg gtggagtcca gggtgtcaaa cagggatgca aattcagcat ccagcgccgc
    35641 ttttttgtag gcttcaaaag tagccttgct gacaattact gctggctctc ggcctctgcg
    35701 ggtgatttca acctcttccc cggcttcaac attgttgagc acttcagaaa ggttgccgcg
    35761 cgcggtacgg aagttaatgg attgcataaa cacctcatgt gctcgttatg tgtacacaac
    35821 tataaacttc acaggtataa agcaccagca ctttgcagct taaatcaccg gacaatcatc
    35881 aaattcccca cttcggacat cattgatgac atgagtgatc acaccaaaaa cagcattact
    35941 ccccgtgcat ccatcgtcat ccactggtaa cgcctctttc ctcccggtgc ttaaatcctc
    36001 caggtgctgg cgcggatact tcctgtatct ctttatgcga tattcaccct ccatagcgca
    36061 cacaagcaga gaaccatcaa ccggagtaag cgaggaatca accaccagca aagcaccctg
    36121 caatattccc tcacgatgat gactatcagc tgccctcatg aagtaggtcg ctgaaggatg
    36181 tctaattatc tgctgatcaa gagaaatacg gctctcagca taatccgccg caggagaagg
    36241 gaagcccata gcgttttcac ctcagaatac tgtttatcca tacagtatac attaaagatg
    36301 cttgaagtgt gcaatagcgc gatgctttgc gctcgcctgg tggtgcgtta ttttttggct
    36361 atatcttctt tctgatacac cggatcgctc ccttttggca actggaggct taactgccgg
    36421 tagtgccgta gccgttccat gaaataggcg cgcaggtttt ccggttgctc acgggccacc
    36481 tgctctgcaa tcacaggtat attcagccgt tctttgtaag caacgccgct ggcggcaagg
    36541 tctacgttca ccttgtctcg ctcttcctgg cttttagctg ctatatttcg atctgacaca
    36601 agaaacctcc aggagttagt ggatcaggca catgcacaat aacgttctca atgttagccg
    36661 ttcaattcag acaatcaggg actgcaccag tgtggtggca tcaatcccat tgctaaacag
    36721 catagacgaa ctgcaaattc gtgtattgaa tccgaatcag gctaataagc aattgcgctt
    36781 tattttgacg ctgaaaagtg aaacagaggg gataaagcac agcgtaaaag tgttttctga
    36841 ggccattctg cttaatggga agctccggca tctggtaagg ccagaacgtc aatatcctga
    36901 cgtcctggcg cgtgaaaaag accttctttc tgaagttcag gatagggtga tcgatttcgt
    36961 caaatgctat cccctgcatt gagggcaggt gttcccttcc gcaaagtagg gaaaactgct
    37021 atcaagttca tcacaccatc tgcgatggtc gtagcaccaa aacgcctccc acggtgcacg
    37081 gaaatacttc atccaccagg acactcgatc aggtatatct gctggtggca gttcttctgg
    37141 caataatttg cctgataatt ccctttccgc ccgcgacagc atttttttga ggctcgcgtt
    37201 ctctttttca agctcatcta cgcgcacctg taattcagct ttcgttggca tggtccgcct
    37261 catgcttttc agccaccagc ggcaataaag ccctggccat cttatgaacc aatagtgcat
    37321 caataatgcc aagcgtatgc cccggcttaa tgtttaatgc cgtctcaagg tgacaccttt
    37381 ccaggccact tttctcggct tgtttatcat gatctggtgt aataacgtcg ctttcccgaa
    37441 agtttccgga ctgaagcatg aatgcgcggc aggcatcctc gacgccctta actgcatctg
    37501 cgcagtagtt atagcgattg cattccacta atttctgctt gagattttca attgattgcg
    37561 cgacatcagc ctgtattacc ggcgctggcg gtgcgatata aaacttcgtc cccaacggca
    37621 acaacttcat cgctttttct cccttaatga tgcgataagt tgatttcccg ccaaggtcta
    37681 ccgttccatc cataacaagg ccgtgtcgct tctctgaaac ttcaccaata ggctctgctt
    37741 ccagcgatgc cagtgcaatc cgtgccagct cacgcacaac ttccggcggc gcgtaacgat
    37801 cattcaggtc atcccacagg cgtagcatgt tatcgctacc agggtgaaca tcctcgttag
    37861 ttccggcaag cgcactaata acctcatcgg ctgcctcaat aattttcaga gcttgttctc
    37921 tggtaatagt ggtcatttgt taatcctcaa aactttatgc ccaggcgcaa aagcacgtgt
    37981 tttgtctttg cttattcgcc agccatcctt acgcgcctct tttgcacaac cagcccatga
    38041 cgtaccgata tactctccga agtctggcac tggatataca ccttccgtac actggcggca
    38101 gtcacaatag agatgcatgg tgtaacttgc agcgatagcc atatcactct cctttgatgc
    38161 gaatgccagc gtcagacatc atatgcagat actcaactgc atcctgaacc cattgaccgc
    38221 caatcccgta atagcgatgc gtaatgatgt cgatagttac taacgggtct tgttcgatta
    38281 acttccgcag aaactcttcc aggtcaccag tgcagtgctt gatgacagga gttttcccag
    38341 gatggcgaac aacaagaaac tgatttccga cttcacggac ttcgttgctt tccagttctg
    38401 caatgcgctt ctctgcggct tccagcttct cgcgcatatc gtcaacgtac tcgaccagag
    38461 aaccgccagc aggaatttcg cactcctcga ccagttggaa gtagatatca gctgcggccc
    38521 gtgtgttgct atgcctagcg tcgcccatct caccttcacg aagagcatcg cgttcggcgg
    38581 taagattggc tattttgctg tctttgcctt ccagctcaac gcgcagcttc cctaccgtta
    38641 gcgcaatatc ctcgttctcc tgatcgcggc gttttatgta ttgctggttt ctttcccatt
    38701 catccaacaa tgccagcgcg atatctggcg aaaagtgctt cataaaatcg ttaagcgcat
    38761 taattcgctg atcgaaaggc attacaggtg cttcaccagc aatttttgtt ttttcagcga
    38821 tttcacgaag cttttgataa tcaatcttgc tcactggttg ccccctgaat acgcttaaac
    38881 tctattaccc acacccaagg attagcgttc caactatctt cgccataaat tgatttccat
    38941 aggctacgga aacctgggta atgcttatcg ccaatgaggg tcgattctgt tggtgcgccc
    39001 tcagcccttg catcgcattc gctgatatcg ttcaaccgct caacgcgcac gttggtaatt
    39061 tccagaagaa tgcgcgatgc ccagcgcggc atgtgaattg atggacgcca cccaccatca
    39121 aacttttcat tcacagtgtg aggtttccag tcggcatcat cgggtatcga ccataaaccg
    39181 taatcaccag gcttttgctc acaactggcc cgataaatcc ttgcagcgtt cttctcatcg
    39241 ccacgacaaa ggttgtcgtt ccagtccaca ctgcaaccat cttcattgcc taatatcgcc
    39301 cacgcctcac ggacccatat gcgatcacca acgttaccga acgggcataa accatgttca
    39361 ggtgcatcca taacgtgggt atagacacca ttgcgtttct ttgtcggttt acggataacc
    39421 aggcagttat cggacccgac attttttaca ggtcggcgag tctgcgtctt ccttccttcg
    39481 aggatggccc tgaccatctc atcgttaaaa atcatcccgc gttctttcat tttttgatcc
    39541 acctcatcag ttattgaaag tgatcactcc gcgctcgatg gcgaagtcaa aaagctggtt
    39601 agcggctacg taaaggcgta tgccatgtgc tttttcccat gcccgaacat cgttttctgc
    39661 gcttctggcg cagtcatcgc aaagaggaac ggcccagcga tcgtgttcgt ttaacgagcg
    39721 ggcgcggtac atgaacgggt ggttaacttt gccaccgcat ccgatgcacg gacgagaaac
    39781 cacaaaccgg agataagccg ggcttttacc gaggaccgct tttggtcggc gcatataaag
    39841 caggccggaa tcctcatcta cagacaggtt gacgatctgc tctgcagtta tgtccaccag
    39901 ctcacgggtg ctgtgctccc aggtgatatc cgattctttc agtgtgccgg tagggatttc
    39961 ctgttttggc tgacaaaatg caatgcgacc ggcttcatct ggcagctcgt ctttcagatt
    40021 ccggcgaatg gcccaaaaag tgagttcaac catgctcaga tcgcgttctg gcggtagttt
    40081 taactcgctc gcagcccagt tcataaccca gttggcacga ttcagggata actggtcatc
    40141 cagcttgccg taccccttca tcatgtattc cgcatcatgc ttccagcaca ggcgaacggc
    40201 agagccgtta tagaaatgag tggttagctg gtggctgcaa tcgcgcttat cgtgcgcctg
    40261 gcaatcgtgg atattggaac ttacccaatg aacaagcgaa tcttcaccgc ctaatgcgtt
    40321 aaatacgcgc tcgctttgaa aaaatgcttt cagagactgg ttggcaacca gcgaatagtt
    40381 cagatccact acgccatctg gcgtgttttc agcctgttcg cgcggaagtg gagagataat
    40441 gaaacggcga cctgcgccaa tgtagttgct ggtgggcttg tctaccggaa agacagccac
    40501 accagctccg tttacgaaat gaggtgtgat tattgcactc ataatgtata ccgactctgt
    40561 tttgttccga gcggtaaaat aatatgttag aaaactaaaa tcaatgttct aacacatttt
    40621 tgagtgcgta aatgatttga tcagacttta agctgccctc ccttcacctg cattagcgtc
    40681 agatttccgc aaaaaaccgc gcccgtgtct atgtagtgct gattccagta tgcttttggt
    40741 cttcgtaccg gtgtgtgccc aaagataaag cgatctgcgc ctgtgatttc tccaccaatg
    40801 ccatctatcg aatcaccgac gcgactgcgc gaccagacaa catcaaaaag cgacacatcc
    40861 ttaccgaact ggtactctcc acctggatag tcggcatggg ctataacgat agtttcatgc
    40921 ccggtgttca gctcaatgat atagggcaga cgctttacca gctccaccag cgccctggct
    40981 aatatttcct gatcagtgtc cagcatgaag aaccattgac cgccattcat tagccagtta
    41041 ttcacattgc ctgcgggact taacgcatcg atcattaatc gctcgtggtt tcccataacc
    41101 gaccgaaacc agggcatctg caatagctcc aggcattcaa cattttcggt gccgcgatcg
    41161 ataaggtcgc caaccgatat cagtaaatcc tgtgtcgggt caaaatccac acgatggagt
    41221 tcattcataa gtagggtgca gcaaccgtgc aaatcaccaa caacccatat gtttctgtag
    41281 cctgaaccat caatacggct atataaattc acttcatgca gcgcctgatt catgcggcaa
    41341 ccttctcccg cagccagata caaaccgggc catcttcagt gtcatgaata gagccaacaa
    41401 accatccttc gccctctggt cgctctggtt cccaggcggc aatatcggga ccatctgcgt
    41461 ccagattaaa atgatcttca tccatactgc gaatagtcca ctgaagatta ttttccttca
    41521 tccaggcgtc aaattcctca gtggatatat attcacgacc tgcgcaaaac ttttcatact
    41581 ccggatgtgt ccagcagcca tattcatcac gttctaccgg catttcttta attgtgttca
    41641 ctcttcatcc tccaaatcag caaccgcgtc catcacatca gaaccgcgaa taacttcaaa
    41701 agcgcggcag gccatttgaa atacccgttg ctcttgcggg tgcggagact cccaatattt
    41761 gaagcctggg cgatgcgtgt accccatcat ggaataaaaa tcgccagcaa gttcaatcgc
    41821 ggcatcaaca agttcgcgat ttgtcatcgt ctgttctgtc atctggtttt ctcctgtctg
    41881 aacatcacta tcatcaggtc gccttttgtc gctatcctcg ctgttgtgcc tggttcaatg
    41941 ctgccaagtt caaatgcgtc atagaacgcc tccaacgctt tctggcgttt ttcctgtttg
    42001 cggcgctttc cccactgctt tagaaaaaca gaggccgccc acttccccgc actgaacatg
    42061 atgaaaaacc aaccgagaag cgccagaccg gtattcagga gcgtatcgat cgttattgtc
    42121 gtgtcgatat tcactggctg cttcctcttc gaagctcatc ggcaaagtca gttgcatgtt
    42181 caccgagtag tttccagtta tcgcgtatat cagatgcaag cgactgttcg gattttgagt
    42241 tacatttctg tgcaaacatc tccaatccct gcgcccggac ttcagccagg aaagcatcag
    42301 tggttggcgt ttcaggtatc tgtctcctca tccgttctat tgcatgattg aacccgaagt
    42361 cttccgcgag agatacgtca tccatattgt cattgtcatc ctcaatatcc cgtgattctg
    42421 gaattgcaga ctttattccc gcattctccg ccgccagcgc cgcgcaatta ccctccagct
    42481 ctgcaatgcg ctgttttgcg gcatcaagtt caatcgataa tttttccaac tgctctttat
    42541 gcttcttgta ttcctgatat gcgtgccagg gctgaccttt gcgcacacta tcagtaatat
    42601 cagtaatctg ttctggtgtt agcgtggtca gtggctgtgc tgggaaaatc agcactttcc
    42661 cggaatccca atcaaaacca gcgtgaattg actgaacctc aactgaaggt gttgaaccaa
    42721 tgctgccagg cgaatgaaca acgatcgtta catccatatc gcgacgatgg ctgtggttgt
    42781 tggacaaaat acgattcacc aactcagaaa atttggaaaa tttcatgctg attccctttg
    42841 atcttcctgg ataatgttgt tgcaatcttt cacgcactcg ttgcatatga aagcgttgtc
    42901 cccggcaatt aactttttaa cggtgtgctg ggatttgttg cagaagctgc aataaagcgt
    42961 tggactggct gatacggatg gtgcgccaga tgtcagccgc gcgatgtcgt tcttgcgacg
    43021 caatatcaca cggctgcact caaccagcat ctccggggat atctcctcct ccgtagagag
    43081 cgtctctaat cgttcgagta tgtggaaggc tttttcctgg gtaatcctga gtaattcagc
    43141 gttacgctct gccagttgtg cgggcgttaa accttcagac ttcatgcact ctcctttcga
    43201 aataaacgta ctgattaatc atgcccaggg gcatttcgag tttttccgcg atctcacggc
    43261 ggggaacacc acactgatga agctgccgtg ccagttctat atcgctctgc cggtatttgg
    43321 ctgacggatg aaagtctcct ttcagaatca tgctgatccc cagctcccgc gctttggtcc
    43381 tgacggcatc accggtacga ccgatcagct tcccaatact ctcgaccgtc atagttccgg
    43441 cgcactggcg gagtatcatg atttctgccc ttccccatcc ccgccagctc acgcagaact
    43501 ccttctaacc aaaagaacgc catcttcaga aacaacgttg caggacttca ggtaattcat
    43561 tgcctctttc ggtaatggat tctttggatc agcgtttgcc agggatttcg ataacctcat
    43621 gataaatttg atcattacct tctggcggag cttgtgctca tgagataaag aaaggtaata
    43681 ggaaatggta tgggctaaac gctcacgaag ctgttctgtg ccgtgatata cagcagcgat
    43741 atcacgcagt gcattgacca gttcccggta aatatgcgga gcaacctggc attcaatgtt
    43801 ggttggtgac tcataaatta cattcagccc cagcttttga gccagctcgt gctctgcacg
    43861 agcgccaact gattcctccc accccttgag caaatagacc gcattagccg aacgcaccat
    43921 tgcaaggcaa atatccatgt attcgctctc gcagaggcca gcaggtaaca gcgccggatt
    43981 cagaacaata tgccctacct ccatcagctc ttttgctttc gaaaaaaacg ctgtgcggtt
    44041 gtagtcggga taaccagtca tcggccccgc gatgtaaatt ttcataatgc gttttgacat
    44101 caaagtttcc ccaggaacgt tacgcaaaag ttgatgagag caaagagttc aatgcccagc
    44161 tcaaacaacg ccaggacagt gaaaaacagg acatatagca acccaatttc tgaaccggat
    44221 tgctggtgga ttgcactaat tgcgacaagc atcatgacct cctgatatag cgtctgaaaa
    44281 aagtatcttc attttgtgtt agaaaatcaa ttttgatttt ctaacatgtt tgatcaacca
    44341 tagcggctat gtagacaaac cgggtatttc tatgaagcaa agctattggg agaaacagac
    44401 acaaaaagcc ctgcaaaaac tggctgatcc gaaatggcga gaggaacaaa gggcaaagcg
    44461 tcttcagcaa gctcaacgcc agcagcagcg tgcgcgagaa aaagccgcat cgcctgaata
    44521 tcggcaaaag aaaattgaaa aagcgaagca atatgaacag cgaagaaaag agaaggccgc
    44581 ttctgctcct gtcaaaaaaa ctcgcgcgtc acgcggcctg aaaggcagaa cactcacagc
    44641 agatgagcgc cggatacaaa ctgccatcgg tgctctcccc tgcattgcct gccatatgca
    44701 cggacagcat agccctgtgg tatccctgca ccacatcttc ggaagaacag cagagaacgc
    44761 gcataaatac gtcctccctt tgtgtaaatg gcaccatcaa cacgcagcgc cagcagagat
    44821 ccgcgagcaa tacccctggt tggttcctgt tcatgctgat ggaaaaatag gcggtaaagc
    44881 agatttcagg cggcacaatg ctgatgaaat gacgttgtat cagatggcga ttgaattgat
    44941 gaattagttt tctaacattt taagttgaat tataaatttc tccgatgtac attcactacc
    45001 gattggcaca ccggtcaact ttttgagaca aactgtatag tttttcgtat tattgccgcc
    45061 gccataccta tggcggtgca atataggtgg ctgaaaagcc cccgttgact cacggtgttc
    45121 cagcctttat tgcgccgccg ccagaccgtg gaacagtcga tggcggctcc gaaagcaagg
    45181 agtcactaca ctatgaacag ctactgtctt ttcccatcac tcgttgtccg ccactcacgc
    45241 gataatctgc attctttact cgcgctgggg gtgtcaaaat gaccgttcgc tacctcaact
    45301 ttcaaatcca gaacatcact ggcggttgct atgactggtt tgtcactctc ggaaaagaag
    45361 tgatcaccgg gaagctggat gaagtgaaag ctaaagcaat ggcctacgcc tgcaagcaag
    45421 cccggaagaa atccgccaaa gcataaaata ctgacttttg cgccctggcc ttatcgtggg
    45481 gtatatttct acggcacctt agaaaaacgg gtgccgggat tggaacctca cgcggtagct
    45541 ccttaactta gtcaaaggag gctgccaata tcgctactat ccccacccca actcattctg
    45601 aatttatctg gcgtttctat tcctgccaaa aacacctgta tatctgcgtc atggctgcaa
    45661 ccgaagcaga agctcgttca taccttcccc tggcggaacg gggcgtaatt ggtgacaaac
    45721 aaatactggg aaacagtgga actacatgag gcgggagtaa ttcgtgacct gatggaggat
    45781 tccgttcccc gccgctatcg tgcagggaac ggcatctccg tggttaaagg tgtttaaagc
    45841 tcacctgcgc gctgtttttc aagcacagat ttaacatttg gcgctttccc tttaaaagaa
    45901 tggaaagtgc cacatttttc tgttttaacc tcgcgcagag cttcgccatc ggcgttctgc
    45961 cattgatggg ataatgtcat atcaccgcga gaaggaacat ccagcgagta cagcgtgtaa
    46021 gttcccccca tctccgcata ctcctggaac acatagctca ttacgtcaga ttcagttttt
    46081 cggtgtgcgt tatcaacctt attcccattc agaaatatgg catctggata gatttttata
    46141 tcaaaccgac cgcacttaac ttgtaaattt ccggcgctcg cgatcaaagg gaaagacgcc
    46201 agtaaagcaa tagccaatct tttcaattct gcctccgctg cgtaacttat tgcccgtatt
    46261 ttttacacca ttgctctgcc atttgtttgg ctaaaggccc ggctactgca ctgtattctg
    46321 ctgttttact ttgcacacat gaagcagatg gttttttatt tcgctgctcg gttagtttgc
    46381 gctcataagc catcatgcta tcgaacccgg caatatcact ttggcgaagc attccctttt
    46441 taaccaaaaa ttgctgcccc tctggcacca acgcaagcat aaaaagaggt tgcaataact
    46501 cacctaatcc ttgctggcga tacacttcga attgctgcca tccctgactt aatctggaaa
    46561 gagtatcttt ggttgaataa cctgtttttg ttatagcttt gtccgtcaga tatgctgtat
    46621 atgcaaaaac gcaaaagtca gttttaccaa taaactgagt tccaacaggc cgtaatggaa
    46681 tgccctccct cttgcaaaag tccataatgg ccgcttgtga atgatctcca ccagcgttat
    46741 tcataacctg ctgccctgcc ctgaattgtg caggtaacgc agttgcattg gcagtattag
    46801 cagatacgca tcccaacata gccgcgagcg cggcccctaa agcccttaac ttcactacac
    46861 catccttcat gcaaacaaaa tgaagtttta tgttataaaa acaattaact acataaacat
    46921 gatttaggcc aaatttcggt taaaaaataa aatccaccaa aagtggttga cactattttg
    46981 gaaatcacaa actgcacata atccatcgcg ctaacggctc ccgttgaagg ttcttttgac
    47041 gattaacttt cagccgaagc gcggtaggga gtcataacgc caaagcaggc cgccatgtgc
    47101 ggcctttttt tgtatccgtc atccgtggag gaaggacaat ggaaaagatc gcaatattca
    47161 gcctgaccac cagcaagcct caaatgctca ctgcaatact gaaagacggt gctctcatta
    47221 ttaacgaagt aaaacccctt cccgcgtcag cattggagca aaagcaaaaa attcctccag
    47281 ctatagcagc cctgcggaaa agcaaattta aggtactggt agacgaaatt acgccaacga
    47341 tctccgctgg caccggagca agccaggtga ctctcaagac ccgtcatgcc gatggcagag
    47401 cagcaatcat cgtcgggatg gaaagataca gagagttaaa actccagaag ctattatccc
    47461 tgccgcaaaa taacaaaggt gctttcgaaa tccctgactc catcgttgac accgaataca
    47521 acggtaacgg agaagaagtc taccgggtga actggcagga tatcaggccg gagcatattt
    47581 tgatgatcct gtgttgctac gcgaccgtat accacaatgt agccagtgcg gattacgtag
    47641 agcagatgac cggtacagtc gagaaagagc aaaaaacagg catactcgct tcgttcctgt
    47701 ccattattgg gcatgaaaaa gttaaagcag gcacctccca gccaaagaca ctgactggga
    47761 aagaagttga tgaagatacc gtgatacttt gatcacatta gtagctttgt taactccctc
    47821 aataccaggt agaagagtaa caaaaatatt gacgccagta tttgcgtggt caatgaagcg
    47881 ttcataaaca gatcaattag cattaataat tcgaagataa catccatgtc attcactccg
    47941 tttaattttt aatctaatgc cagcaaatga agctggcccc cgcataaaga ttaatgaaag
    48001 tcacttgtca ccagtgaggg gatttatgaa ccacatcccc ctgaactggt ggcctgtctg
    48061 tttctcgcat ttcacaccct gcatacagtt tccccc
//
LOCUS       DAJVRP010000022        47825 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVRP010000022 DAJVRP010000000
VERSION     DAJVRP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.022"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(53..128)
                     /locus_tag="OQN12_001240"
     tRNA            complement(53..128)
                     /locus_tag="OQN12_001240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93..95),aa:Val,seq:tac)
     gene            complement(265..340)
                     /locus_tag="OQN12_001241"
     tRNA            complement(265..340)
                     /locus_tag="OQN12_001241"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(305..307),aa:Lys,seq:ttt)
     gene            complement(505..1296)
                     /gene="cpoB"
                     /locus_tag="OQN12_001242"
     CDS             complement(505..1296)
                     /gene="cpoB"
                     /locus_tag="OQN12_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1653642.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1306..1827)
                     /gene="pal"
                     /locus_tag="OQN12_001243"
     CDS             complement(1306..1827)
                     /gene="pal"
                     /locus_tag="OQN12_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1653643.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1862..3154)
                     /gene="tolB"
                     /locus_tag="OQN12_001244"
     CDS             complement(1862..3154)
                     /gene="tolB"
                     /locus_tag="OQN12_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1653644.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3287..4528)
                     /gene="tolA"
                     /locus_tag="OQN12_001245"
     CDS             complement(3287..4528)
                     /gene="tolA"
                     /locus_tag="OQN12_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1653645.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4593..5021)
                     /gene="tolR"
                     /locus_tag="OQN12_001246"
     CDS             complement(4593..5021)
                     /gene="tolR"
                     /locus_tag="OQN12_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1653646.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5025..5717)
                     /gene="tolQ"
                     /locus_tag="OQN12_001247"
     CDS             complement(5025..5717)
                     /gene="tolQ"
                     /locus_tag="OQN12_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1653647.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5714..6118)
                     /gene="ybgC"
                     /locus_tag="OQN12_001248"
     CDS             complement(5714..6118)
                     /gene="ybgC"
                     /locus_tag="OQN12_001248"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1653648.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6268..6561)
                     /gene="ybgE"
                     /locus_tag="OQN12_001249"
     CDS             complement(6268..6561)
                     /gene="ybgE"
                     /locus_tag="OQN12_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1653649.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6561..6674)
                     /gene="cydX"
                     /locus_tag="OQN12_001250"
     CDS             complement(6561..6674)
                     /gene="cydX"
                     /locus_tag="OQN12_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1653650.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6689..7828)
                     /gene="cydB"
                     /locus_tag="OQN12_001251"
     CDS             complement(6689..7828)
                     /gene="cydB"
                     /locus_tag="OQN12_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1653651.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7844..9412)
                     /gene="cydA"
                     /locus_tag="OQN12_001252"
     CDS             complement(7844..9412)
                     /gene="cydA"
                     /locus_tag="OQN12_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1653652.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10261..12894)
                     /gene="mngB"
                     /locus_tag="OQN12_001253"
                     /pseudo
     CDS             complement(10261..12894)
                     /gene="mngB"
                     /locus_tag="OQN12_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(12912..14828)
                     /gene="mngA"
                     /locus_tag="OQN12_001254"
     CDS             complement(12912..14828)
                     /gene="mngA"
                     /locus_tag="OQN12_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1653653.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14998..15720
                     /gene="mngR"
                     /locus_tag="OQN12_001255"
     CDS             14998..15720
                     /gene="mngR"
                     /locus_tag="OQN12_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1653654.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15823..16692)
                     /gene="sucD"
                     /locus_tag="OQN12_001256"
     CDS             complement(15823..16692)
                     /gene="sucD"
                     /locus_tag="OQN12_001256"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1653655.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16692..17858)
                     /gene="sucC"
                     /locus_tag="OQN12_001257"
     CDS             complement(16692..17858)
                     /gene="sucC"
                     /locus_tag="OQN12_001257"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1653656.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17952..19169)
                     /gene="odhB"
                     /locus_tag="OQN12_001258"
     CDS             complement(17952..19169)
                     /gene="odhB"
                     /locus_tag="OQN12_001258"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1653657.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19184..21985)
                     /gene="sucA"
                     /locus_tag="OQN12_001259"
     CDS             complement(19184..21985)
                     /gene="sucA"
                     /locus_tag="OQN12_001259"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1653658.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22286..23002)
                     /gene="sdhB"
                     /locus_tag="OQN12_001260"
     CDS             complement(22286..23002)
                     /gene="sdhB"
                     /locus_tag="OQN12_001260"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1653659.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23018..24784)
                     /gene="sdhA"
                     /locus_tag="OQN12_001261"
     CDS             complement(23018..24784)
                     /gene="sdhA"
                     /locus_tag="OQN12_001261"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1653660.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24784..25131)
                     /gene="sdhD"
                     /locus_tag="OQN12_001262"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24784..25131)
                     /gene="sdhD"
                     /locus_tag="OQN12_001262"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1653661.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25125..25529)
                     /gene="sdhC"
                     /locus_tag="OQN12_001263"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25125..25529)
                     /gene="sdhC"
                     /locus_tag="OQN12_001263"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1653662.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25910..26017
                     /locus_tag="OQN12_001264"
     CDS             25910..26017
                     /locus_tag="OQN12_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653663.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26223..27506
                     /gene="gltA"
                     /locus_tag="OQN12_001265"
     CDS             26223..27506
                     /gene="gltA"
                     /locus_tag="OQN12_001265"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1653664.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27897..28463
                     /locus_tag="OQN12_001266"
     CDS             27897..28463
                     /locus_tag="OQN12_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1653665.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28514..30973
                     /locus_tag="OQN12_001267"
     CDS             28514..30973
                     /locus_tag="OQN12_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1653666.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30988..31719
                     /locus_tag="OQN12_001268"
     CDS             30988..31719
                     /locus_tag="OQN12_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1653667.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31716..32792
                     /locus_tag="OQN12_001269"
                     /pseudo
     CDS             31716..32792
                     /locus_tag="OQN12_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32944..33990
                     /locus_tag="OQN12_001270"
     CDS             32944..33990
                     /locus_tag="OQN12_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1653668.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33987..34778)
                     /gene="nei"
                     /locus_tag="OQN12_001271"
     CDS             complement(33987..34778)
                     /gene="nei"
                     /locus_tag="OQN12_001271"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1653669.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34814..35548)
                     /gene="pxpA"
                     /locus_tag="OQN12_001272"
     CDS             complement(34814..35548)
                     /gene="pxpA"
                     /locus_tag="OQN12_001272"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1653670.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35538..36470)
                     /gene="pxpC"
                     /locus_tag="OQN12_001273"
                     /pseudo
     CDS             complement(35538..36470)
                     /gene="pxpC"
                     /locus_tag="OQN12_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36464..37120)
                     /gene="pxpB"
                     /locus_tag="OQN12_001274"
     CDS             complement(36464..37120)
                     /gene="pxpB"
                     /locus_tag="OQN12_001274"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1653671.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37143..37886)
                     /gene="ybgI"
                     /locus_tag="OQN12_001275"
     CDS             complement(37143..37886)
                     /gene="ybgI"
                     /locus_tag="OQN12_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1653672.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38157..39638
                     /gene="dtpD"
                     /locus_tag="OQN12_001276"
     CDS             38157..39638
                     /gene="dtpD"
                     /locus_tag="OQN12_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1653673.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39788..41206)
                     /gene="phrB"
                     /locus_tag="OQN12_001277"
     CDS             complement(39788..41206)
                     /gene="phrB"
                     /locus_tag="OQN12_001277"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1653674.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41203..41712)
                     /locus_tag="OQN12_001278"
     CDS             complement(41203..41712)
                     /locus_tag="OQN12_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1653675.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41841..42128
                     /locus_tag="OQN12_001279"
     CDS             41841..42128
                     /locus_tag="OQN12_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCS1653676.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42183..42563)
                     /locus_tag="OQN12_001280"
     CDS             complement(42183..42563)
                     /locus_tag="OQN12_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653677.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42566..47230)
                     /locus_tag="OQN12_001281"
                     /pseudo
     CDS             complement(42566..47230)
                     /locus_tag="OQN12_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(47249..47452)
                     /locus_tag="OQN12_001282"
                     /pseudo
     CDS             complement(47249..47452)
                     /locus_tag="OQN12_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11371 a        13122 c        11599 g        11733 t
ORIGIN      
        1 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc ggtggtgggt
       61 gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct
      121 aatcacccga cactacgctg gatactacaa ctaactatgt tagtggtggg tcgtgcagga
      181 tgactcgact tcgtctcgcc cttcggcgcc gttgctgatg caacgttatc cttcacgttt
      241 tactaccgct tccaccttag cgattggtgg gtcgtgcagg attcgaacct gcgaccaatt
      301 gattaaaagt caactgctct accaactgag ctaacgaccc actttttcgt tgctttcggt
      361 ttgtttgata tcccgtggca acggcggcat atattactga tttcagactt gagcgcaaca
      421 aaaatttcga tgcagatcac tcaactgctt atgattcgca cgacacgacc agaaataatg
      481 cgacttctgg tcgtgtgtta tgcattacat cgcgttcaga cgtttttgtg cctgtttagc
      541 gccatcagta ccagggtatt tactgataac ctgctggtat acggctttcg cttttgcggt
      601 gtcacctttg tcctgcatga tgacgccgac tttaaacatc gcatcagcag cctttggtga
      661 cttcggatag tttttcacta ccgaagcaaa atagtacgcc gcatcatctt ttttaccctt
      721 gttgtagttt aactgaccca gccaataatt ggcgtttggc aggtaagttg aatcagggta
      781 atttttgatg aaattctgaa atgccaccat tgcgtcatcc tggcgggatt tatcctgcac
      841 cagcgcaata gctgcattgt aatccgtgtt tgcatcgccg cttttcaccg gcgcgccagc
      901 attcgcagta ccagcatcag ctgtcggcgt cgttgatgcc gtcgcaccgc tttgatcgct
      961 gctggttgat tgcgccgctg caccaccgct gctgaggctg tcgatctgca acaggatctg
     1021 cttttgccgc tccacgacct gattcagttg atactgattt tcctgaatct gaccacgcag
     1081 ggaatcaata tcggattgat tatcagaaag ttgttgctgg agttgggtta aaagctggct
     1141 gtgagcatta gaaatacgct caagttgagt gacgcggtct tcgaccgagc ctgagccgac
     1201 actactgatt ggtgcctgag caaaagcggc ccagggggcc gctataccaa ccagtaacga
     1261 cagactcaat agttgatgtc tgaagttact gctcatgcaa ttctcttagt aaaccagtac
     1321 cgcacgacgg tttttggagt atgccgcttc gtcatgaccc agtactgcag gtttttcttt
     1381 accgtaagaa acgatggaga tctggtctgc agaaacgcct ttaccctgca ggtacatctt
     1441 aacggcgttc gcacgacgtt cacccaggga gatgttgtat tccggagtac cacgttcgtc
     1501 cgcgtgacct tctacggtga ctttgtaaga cgggttgcta cgcaggaagt ttgcatgtgc
     1561 atccagcatt tgagcgaagt cagaacggat atcgtacttg tccagatcga agtaaacgat
     1621 gttgttctgc tgcagctgtt gcatttgcag acgagcctgc tcttcggaag acatgttgcc
     1681 gttgccgccg ttcgcatcca taccagtgcc ggcacccagc atgccttcgc tgccgtcatt
     1741 gctggcgttc ttgttggaag aacatgccgc aattgccata acaggcagag caatcatcag
     1801 ccctttcagc actttgttca gttgcatttc aatgattcct ttactattca attaattatt
     1861 atcacagata cggcgaccag gcagggaatt tgacctgtcc atcagttgcc ggaagacgcg
     1921 ctttgaaacg cccatctgta gaaaccaaat tcagcacgga tcccatcccc tgagaagagc
     1981 tgtagattac catagtgccg ttaggtgcca gacttggcgt ttcatccagg aacgtggacg
     2041 acagaacttg tacgcctccc gttgccagat cttgtttggc aatgtgctgc tgcccaccat
     2101 tggagctgac cattaccata aatttaccgt cgctgctgac atccgcatcc tggttctgcg
     2161 aaccttccca ggtaatacgt tgtggcgcac cgccgttgat attcacttta taaacctgcg
     2221 gacgaccggc ctggtcagaa gtaaatgcca ggttctggct atccgggaac caggtcggtt
     2281 cggtattgtt actgcgacca tcagtcacct ggcggatctg accagaagcc aaatccatta
     2341 cgtacaggtt cagactaccg gttttcgaca aggcgaatgc cagtttgctg ccgtctggcg
     2401 agaatgcagg tgcaccgttg tgacgcggga atgaagccac ctgacgtaca gcgccatttg
     2461 ccagcgtctg aataaccagc gcggaacgac cgctttcgaa ggtcacataa gccagtttag
     2521 aaccgtctgg tgaccacgcc ggagacatca gcggctgcgg tgaacggtga acgacaaact
     2581 ggttgtaacc gtcatagtca gatacgcgca gttcatacgg gaactgaccg ccgttggtct
     2641 gaacaacgta ggcaatacgg gtacggaacg cacctttaat gccggtcagt ttttcaaaca
     2701 cttcatcact ggcggtatga ccagcataac gcagccactg cttgttcact ttgtacgagt
     2761 tctgagcaag tacagtaccc ggtgcgccgc cagtgtcaac aagttgataa gcaacattgt
     2821 aagaaccatc cggattcgga gtgacctgac cgacaactac agcgtcaatg cccagtgcgg
     2881 accatgcagc tggttgtact tcctgcgcac tacccggctg ctgtggcaga cgagcgcgat
     2941 ctaacggatt aaatttaccg ctgttacgca agtcagcagc aacgatgccg ccaatatctt
     3001 caggtgccgc accaggcccc gcccactgga aaggaacaac accaatagga cgaccggaat
     3061 ctacaccgct gtcgatcaca atgcggactt cagcatgcag aactgatgcc cacagtatga
     3121 gaaaaccaaa tgctactcgt aatgcctgct tcatcatatc tcccttatct ggaccgatgg
     3181 cccacgataa tttagcagaa tgttaacaaa ctaaaataca caaaactacc agaaccccgt
     3241 ggcaaccggt gcctgatgtt gaccgtccga acagtcaaca tcgcgattac ggtttgaagt
     3301 ccaatggcgc gtttttgaac acttcatata ctgcctggct tggtggtttc gggatcttcg
     3361 caagtttagc tgctgccaac gcagcctgac aaagtgcggg atcgccacct tcaggtttga
     3421 tatccagtaa cataccatcg ggtgccagtt ttatgcgcag cgtacaggtt ttgcctgcat
     3481 aggacgatgc gtcatagaac ttactttcga tagcagattt aatctgcccg gcatagttat
     3541 tgatatcggc ccctgatgcg ccattgtttt tagtattacc actcccggca ggcgaagcat
     3601 tgttcccttt cgcccctccc cccgttttcg gtgcattctt accagagctt agctcaccga
     3661 aaatatcatc tgcctctgcg gcagcttttg ctgcagcggc tttttcagct gctgcttttg
     3721 ccgctgccgc ttttttgtct gcggcggctt tttctgccgc tgctttctta tcagctgctg
     3781 ccttatcagc agccgctttt tctgctgctt ttttgtcggc tgcagctttc tctgctgccg
     3841 ctttcttatc agctgcagcc ttttcagcag ccgctttctt ctcagcttct gccttggctt
     3901 tttcagcagc ttcagttgct gctttctttc ttgcttcagc tgcagctgct tctgccgctt
     3961 ccgctttctt cttcagtgcc gcagcggcta cctcggcttt tttctgcgct tcggctgcgg
     4021 ctttggcggc ttctgcttct gcttttttct tcgcgtctgc agccgctttc ttcgctgctt
     4081 cttccgcagc tttagcatct gccgccgctt tcgctgccgc cacttcagct tgcttctgct
     4141 ttaactcggc ctgttttgcg gcttcttcag cctgcttttt ctgctcctga gccgctaacc
     4201 gctctttctc aagttgcttc aggcgttcct gttcagccgc ttgtttctca cgcagttctt
     4261 cagcagcctg ctgttccttc atcttgcgct gttcatcaga acgcttcgcg cttgattcct
     4321 ggctttgcat gcgtttgtac tgctcaacta ccgcacctga atcaaccatg acagcgtcga
     4381 tggacgaacc accgccgcct ccggctgaag cttctatatt ctcatcgaac gaactccaga
     4441 tcagcgccgc aaataagatg acatgcagca ctgctgaaat aattatcgcc cgcttgagct
     4501 tgtcgttttg ttcggttgcc tttgacactc tcggtttcca aaaactgttc gcctgttacc
     4561 cgctctcttt caagcaaggg aaacgcagat gtttagatag gctgcgtcat taaaccaacc
     4621 gatttcacac ccgcactatg taacaagttc agtgctttaa ttatttcatc gtaaggcaca
     4681 tcttttgcgc caccgatcag aaagaccgtt ttcgggttgg ccttgaaacg gctggacact
     4741 tccgccacca cctgctctgg tggtaaacgc tccaggcgat ctttctcaac caccacggtg
     4801 tactgaccaa taccagacac ttcaacaatc actggcggat tatcgttact gctcaccgcc
     4861 tgtgattcag tagcgtctgg cagatcgacc tccacgctct gggtgatgat gggcgctgtc
     4921 gccataaaga tcagcaacag caccagcagt acgtccagca acggaacaat gttgatttcg
     4981 gacttgagat cgcgacgacc tcgtccacgc gctctggcca tggcttaccc cttgttgctc
     5041 tcgctaacgg taaacgcctg gcggtgcaga atcgcggtaa actcttccat aaagttgtcg
     5101 taattcagtt ccagtttgtt tacgcgctgg ttgagacggt tataagccat taccgccgga
     5161 attgcggcga acagaccaat tgccgtcgca atcaacgctt ctgcgatacc gggcgcaacc
     5221 atttgcagtg ttgcttgttt taccgccccg agggcgataa aggcgtgcat gatcccccag
     5281 accgtaccaa acagaccaat atacgggctg atggagccaa ccgtaccgag gaacggaatg
     5341 tgcgtttcca gattttcaag ttcacggttc atggagatac gcatagcacg cgacgcccct
     5401 tccactacgg cttccggcgc atggctattg gcacgatgca ggcgcacaaa ctctttgaac
     5461 ccgctgtaaa agatttgttc cgaaccagtc agattatccc gtttcccctg actctcttga
     5521 tagaggcgag agagttcgat tccagaccag aatttatctt caaacgcttc ggcttcacgc
     5581 gccgctgcgt taagaatacg ggttcgctgg ataataatgg cccaggatgc gattgaaaaa
     5641 ccaatcaaaa tcaacatgat aagtttaacc agaaggctag ccttcaggaa caaatcaagg
     5701 atattcatgt cagtcactgc ttaaactccg cgacaataga cttgggaagc gcacgaggct
     5761 tcattttgag tgggtcaacg caaacaacca gaacctctgc ttcattcagc aaagtattct
     5821 cggcgttgac aatacgttgc gtgaaaacca aagaggtgcc acgcattgat gttatttcag
     5881 tctgtatttc gagcatatcg tcgagccgcg caggtgcgta atattccacc gtcattttac
     5941 gtaccacaaa ggcaacgcgt tcagccatca gcgcctgctg actgaagtga tgatgacgca
     6001 gcatctctgt gcgtgctctt tcataaaaag cgacgtaact ggcgtggtac accacaccac
     6061 cggcatcggt atcttcatag tagacgcgaa ccggccatcg aaacagcgtt gtattcactt
     6121 tacatcccgg taatgcaaca aaagttagag cttttaaacg ctgctactat acgcgcgcga
     6181 ttgaggtttg ggaatgaggg caagttaagg gcgcgtaaaa atttatgggc ctggagcggc
     6241 ccataatttt ttaaagagaa ttctgattta aaagaagaag aaaatcagcc cgacaatgag
     6301 aacaatatcg gcaagcaatg ggcaaaaaat cccttgccaa agaaccttct gcggacgaaa
     6361 gcccacgccg tgaatcacac cagcacagac ggcccacatc agcaataaac catgccagat
     6421 ttccagttca ctggtcttcg cggcaaaacg agatgggtcc caaaacatac atcctgctaa
     6481 cagaagcgcc atcacgaagg aaagcgcccg cagggggcgc ttgtccatta ccgcatacaa
     6541 ggtcgcgata atcttactca tcagatgtct tcttgaccgg ctttgcctga ttcgacgtgt
     6601 tcaagcgcca gcgcggtgat taccccaaac gaacaggcaa gaagcgttcc cagaatccat
     6661 gcgaaatacc acatttttag ctccttactt agtacagaga gtgggtgtta cgttcaatat
     6721 cttctttggt gatacgaccg aacattttcc agtaacacca ggcggtgtag agcagaatga
     6781 tcggtaccag aaccaccgca acccaggtca tgacgttaag cgtcagctgg ctggaagttg
     6841 catcccacat tgtcagactt gcgttcatca tggtgctgga cggcatcaca aacgggaaca
     6901 ttgcgatacc ggctgtcagg atgatgcagg ccagcgtcag ggaggagaac acaaacgccc
     6961 acgcggcttt atccatacgt gcagtcagga tggtcagcag cagcagaacc acacccagtg
     7021 ccggaatagc ccacagaatt ggcgtgttgt tgaagttaac cagccatgcg ccagcttcac
     7081 gaaccacttc tttattcagt gggttagagg ctgcgtaatg gtccattgtc gatttcacga
     7141 cataaccatc gataccgtac atcacccata cgccagccag tgcgaaacag accagtgtca
     7201 ccagcgcagc cacctgagcc gttgcacggg tacgcaggtg cagttcgccc acggtacgca
     7261 tttgcagata ggttgcgccc tgagtaatga tcatccctac gctcaccacg cctgccagca
     7321 ggccgaacgg gttaagcaac tggaagaagt taccggtgta gtacagacgc agatattcat
     7381 caacgttgaa cggtacgccc tgcaacaggt taccgaacgc tacaccaatt accagcggcg
     7441 gaacgaagct accaatgaag atgccccagt cccacatgtt acgccagcgg gtttcttcaa
     7501 tcttggagcg gtagtcaaaa ccgaccggac ggaagaacaa agacgccagc acgaggatca
     7561 tcgccacata gaagccggag aacgcagcgg catagaccat cggccaggca gcaaagagtg
     7621 cgccgcccgc ggtgatcagc caaacctggt taccgtccca gtgtggtgca atggagttaa
     7681 tcataattcg acgctcggtg tcgttacgac cgaggaaacg ggtgagcatg cccaccccca
     7741 tgtcgaaacc gtcagtgact gcaaaaccaa tcagcagaac gccaaccagc agccaccaga
     7801 taaaacgcaa tacttcataa tcgatcattt gacgactcct gtcttagcgt gccggctgag
     7861 tagtcgtgga agactgctca aagtgatagc gaccggtttt caggctgctt gggccgaggc
     7921 gtgcaaactt gaacattaag aacaattctg ccaccaggaa cagggtatac aggccgcaaa
     7981 tcagcaccat tgagaagatg agatcgcctg cggtcagtga cgaattcgcc acagctgtcg
     8041 gcagcacttc accgatagcc cacggttggc ggccatattc agccacgaac cagcccgctt
     8101 ctacagcaat ccacggcagc ggaataccgt acagcgcggc gcgcagaagc cattttttct
     8161 cgccaatgcg gttgcggatg acactccaga aagagagcgc gatgattgcc agcagcagga
     8221 agccacacgc caccatgata cggaacgcaa agtacagcgg cgctacacgc gggatggagt
     8281 ctttggttgc ctgttgaatc tgcgcttcag tcgcatcagc cacgtttggc gtatagcgtt
     8341 tcagcagcag accgtaaccg aggtctttct tcatgctatt gaactggtca cgaaccgcct
     8401 ggtcggtaga accagaacgc agttgttcga gcagagagta cgccttcatc ccgttacgaa
     8461 tgcgttcttc atgctgcacc atcagctctt tcaggccgat aaccggggta tccacggaac
     8521 gcgttgcaat gatgcccagt gcgtaaggga tctgaatcgc aaatttgttc gtctcctctt
     8581 cctgatcagg aatgccgaac aaagtaaagg cagcaggcgc aggttgcgtt tcccactcgg
     8641 cttcaatagc agccagtttg gttttctgca cgtcgcccat ttcgtagccg gattcatcac
     8701 ccagaacaat aacagacaga acagcagcca taccgaagct ggcagcgata gcaaaggagc
     8761 gtttagcgaa ggcgaagtca cgacctttca gcatatacca tgcgctgata ccgaggatga
     8821 acatcgcgcc agtcacataa ccagacgcta cagtgtgaac gaatttcacc tgagcaaccg
     8881 ggttaagcac cagctcggag aagctcacca tctccatacg catagtttca aagttgaaat
     8941 cggacgcgat tgggttttgc atccagccgt tcgcaaccag aatccacagt gcggacaggt
     9001 ttgaaccgag cgccaccagc caggtgacac acatatgctg aactttaccc agacgatccc
     9061 aaccgaagaa gaacagacct acaaaggtgg attcgaggaa gaaggccatc agaccttcga
     9121 ttgccagcgg cgcaccgaag atatccccta catagtggga atagtaagac cagttagtcc
     9181 cgaactggaa ctccatggtc agaccggtag ccacacccag agcgaagttg ataccaaaca
     9241 acttgcccca gaacttggtc atatctttat aaatctgttt gccggagagg acgtagaccg
     9301 tttccataat ggccaggagg aacgccatac cgagcgtcag tggcacaaaa aggaagtggt
     9361 acatcgcggt caaggcaaac tgtaagcgcg acagttcgac tatatctaac atcatgactc
     9421 cttgctcatc gcatgaagac tccgagagtg aaccccgtta gaaagggtca cacgcatgcc
     9481 ccaatagaaa tttgtttgaa cactttcatc ataccatgtg aatgcacaca aaaacggcgt
     9541 aaagaaaggt tgcaaacatg ttaataaaaa ctcaaattga tcccacgtat atattacgcc
     9601 gcaaaatcct tacaataaac aggtttttgt tgaagcaaat ttgcattttt cgacggtgat
     9661 caatttatag ctaaattgcc gcctttcagc caatttgatc gagaacaatt tatctctttt
     9721 tgatgcccat ttccaagact tatacattga taaatatcaa tttctacaaa ttatgttaat
     9781 catgtttcaa caaggtaact ttagtgatat ttatgtttaa aatgtgtgaa ttataagact
     9841 gaaattagtt tcggttggca attaaagata aaaagagacg aagcaaaaac agcacggttg
     9901 accttaattc tttttttggt cactggctaa tacgacgcag agtttcagga ttattaataa
     9961 aggcataaat ctcccccccg ccgatattaa catttaatta acttataatt atattataat
    10021 taactcaccc gtggttatta ttttgttaaa aataaagcca catatacgta gcttgagagt
    10081 gaagataaac acaaagttac ctgatgcgct acgcttgcca gtgtctacat catctctgca
    10141 atatattgaa tttgcctgct tttgtagacc gaataaagcg ttcatgccgt atccggcatg
    10201 aacaaagcgc cctttgtcaa caatctggcc gcgcatatgt ggcttcggtt tgattgctat
    10261 tcaggcaagc cggtaactga acgtccgtga ctggccgggt aaaaaaggcc ccgatagatt
    10321 tgaaccttga ttttcctcgg tggtaatgtg ttcatccatc atcgtttctg agcaagaaat
    10381 cacctcgcga ctgaaagcaa cagtcgcatc acaggttgct gattcagccg gattaaacag
    10441 ccgtaaaatc acttcttgtc ggtcttcagc tttcttaagt gcgcttatca ggcatcccac
    10501 tgggggcatt ttcaacaaac tataactttc cggcacgttg aatccggctt tgttgagctt
    10561 catcgcatcc catgggattt tgttgtaaca ctgtactgga gtcagccatg ctcgcgcctg
    10621 ctgagctaca ccagcggcgg ttggcgtacc ggtataactc aataaactta ggcgacaaga
    10681 aagcagacca cgtagttgtg agtccgggac tggcatttta attcccgaag gccgcctagg
    10741 ccttaaaagc agatcttctt tccccagtaa gcccacgccg cgcagcaacg taatggcaaa
    10801 ggttttcttc tcttcaccga tgacttcaaa ttcacgtagc ccttcgctaa agacagccat
    10861 gccgttacgc ccttcctgta aggctgcata gttgagcata ttccataccg gaaccggcgc
    10921 ttctttccag ccttcttgct gccagttgtt cattgcactg tcgttcacgg ggcgcgttag
    10981 cgaaccaaac tgggtatctg ccagaacact atcggtgtta aaagatgtag ggatcaggac
    11041 gcgaaggcga tgatcgtcag cctggttatc aaggttgata tccacatcaa tgcgcctgtt
    11101 gttatgacta agagtgacaa ccaactcgac atctactctg ccagtggatt gtcttgcgct
    11161 gcgttgttgc agattacgcg gcaccgccat ttcatagcgg ataacagccc tgctctgcca
    11221 ggcctcatga ataatctcgc attgcggttt cgcattcgcc gaagtaatta cccactcttc
    11281 ttttgcgggt gaatagtcat attcatcacc atcatcagag ctttcttcaa tttgcaacac
    11341 ccggtcatag cgcacaccgc tatctttatc taccagttgt agcgttccat cctcattgag
    11401 cgcaatttgc cagaaagcat tttccagtat cccttcagcg tcactttttg ccgcaatcac
    11461 gttgccaggc tgattcgctt cgatataaag cgtgcgatag cccatagaag ggacaatctg
    11521 gttgatctgt atatcaaact ccataaaggg atcgtaatta ccgtaatgaa ctatttgccg
    11581 atcgattagg cctggatcga tctcacgggc atggcgaata aaatacggta caggctgacc
    11641 gcgatcgtct cgtagattaa actggctggc gcgcagccgc acagtggtgt tgatcacttc
    11701 ttcccgcggc cagggcatca ggttaaacag gacgagtttg tcggcgtcgc tctgcggcat
    11761 gttgtcggcg attttacgca tatagaaacg cagcagatta tccgccatgt cttcagccag
    11821 ttcgaagcgg gcgacgattt cgcgatgaac tttgtcacta cagcagcagc cgatgctgtc
    11881 gtgggcatga ttttttaaga tctctttcca cattttctcc agcaagccgt ggtggtatcc
    11941 aaaacccaac gtcctggcca gcgttgccag cggttccagc agattaacaa tcttattttc
    12001 aatacgtgcg tgggcaactt tgatatccat gcgcgtggaa ccgatggtgc gatgcacgcg
    12061 catatatttg ccatcaataa attccccttt cagggttgcc agattatctc gctgcgcttc
    12121 gatcttctca aatacctctt caaagcggct catcacaaac tttcgttgag gataaatttc
    12181 gcgcagtttc tccatcactt cgaagatatt ttgctgcaat ggcatctgat catgcccatt
    12241 cggcagcaaa atctctttgg ttacagacgc tttttccagc acgtcaaaat aactgtcgag
    12301 gcgtttgcgt aatccgtttt cgtccgcagg taagtactta ccgatggcat aacccaacgg
    12361 catcacctgc gccgtcactt cgctaccgtc actgctttgc cacaaaaact cggttttatc
    12421 cgtaccgtgg cgctcagaac atccacgcca gaacatggtg caggtaatgc caaatccatt
    12481 gtagatatgc ggcagttgcc cggacatgcc aaaggaatcg ggtaaataac ctattttcat
    12541 cggctcgcca aacgcgaggc agtcacgcat tccgtacatc agattacgga cgatggattc
    12601 cgcagaaaca atcgtggtat cggtctgggt atactagggg ccgataatca acttgccggc
    12661 ttctaccagt ttcttcacgc ggtctttgtt ttccggtttc accgcgaaat aatcttcgag
    12721 gatcgccgtt tgcccgtcga gtacgtaata tttgtattcg ttgtcctgtt ccagtcggca
    12781 caggatctct tccatattat tgaccagcag aatgcgtgac tcttcggtgg tgaaatacca
    12841 ctcccgatcc cagtgcatat gcggggtgat gtgaacgcga gataccgctt tcatcgtcgt
    12901 ttcctttttg tttatggcat tacgccatca gtcagatagt tgccatgctt aaccgcgtga
    12961 cgccgccaga tcaggagaat tgcagtcgag attgcagccc ccaccaatgc cgcaccaaac
    13021 cagccaattg ccgccataac accgcccgcg ccattatcat gaagtaaaaa gagcgagaaa
    13081 atgccagcac cgggtgtcga aaggccgata ttcatcgcac cgacaatagc gcccgttacc
    13141 atagagccca gcacaaacga accaataacc cgcagcggat cttcaatcgc catcgggatc
    13201 gccccttcgg taatacctgc cagccctaac agccaggtgg atttcccggt ctcaatttca
    13261 aactctttaa acaggcgcgg tgcgagcatc gtggaagcgg ttacggtaaa tgccgaaacc
    13321 attttgacgg aggcgaaaat ggcatacggg ccgtaaacgc cgttcgccat tgcgcccagg
    13381 cagaatgcat aagcggcttt attcactggc cctccaaggt caaaggaaca cataaaaccg
    13441 agaatggcac ccagcaacag cgcgttactt cctgacagac cgttcagcca ggcggtaagc
    13501 gagttattga tccaggcgac aggttccccc accacaaaca gcatcagact gcccgctccc
    13561 aatgtaccga gcaccgggta gagataaaaa gtcaggaagc cattgaattt actgctaaga
    13621 cgcaagtgat ttttcatcca gcgtatcaag taaccggcta tcaatccgcc aacgaccgcg
    13681 ccgagaaacc cggagccgat catgttggcg gcaagtccag ccgcaaagcc tggcgctaac
    13741 gccggtttat ctgccagcga ataggcggta taggctgcca gcaccggtac catcaaaatt
    13801 ccgagcagcc cgccgcccag tttgcggtac atccacagcc aggagttttc ttcattaaac
    13861 agatcttgta gcccgaagat ttgcgacagt aataccgcga ccgccagcac cgtccccccc
    13921 gcgacaatca acggtacggc aaaagagatc ccgctcaaca gtgcctgttt cagctccgtt
    13981 ttgacgcttt tcaccggttg cgtatcttgt tgtaaggtac gcgtctcatc gccacgctcg
    14041 agggtaagcg attgttggat caacgcttct gcatggcgaa tcggctcggc aacaggcaca
    14101 gaaagcgcag gaatgccgtt aaaacgctca ctctccttga tggcgacttc agccgcaaaa
    14161 atacaggcag ttgcactatt gagttgatcc gccgttaaac gcccttcaat accgttagca
    14221 ccttgctttt caacgtagac attcacgccg agtttgcgtc cggctttttc cagatactcc
    14281 gcagccatgt aggtgtgagc aatacccgcc gggcaggccg ttacgcaaac gatagttggc
    14341 gcgttggaaa aagaggcaga aggttgcgtg cctcccttgt catccagcgc cgagagcaat
    14401 tcatcaggcg tcgtcgccga ctgtatacgc gcccgaatct catcatccgc caggcgcgtg
    14461 gtcagcgctg tcagcagttg catgtgcgta gtacccgctt cattgagggg aatcgccagc
    14521 aacaccacta aatcaaccgc ttccgggcca tccacgcctt cccactgaag cggctcgctg
    14581 agtgtggcga ccgcaaacgc cgcttctttt accgcagcag ttttgccatg cggcacagcc
    14641 aacccttcac ttaaggcggt cgggccaagg ctttcacggc gatacacttc tttcagaaat
    14701 tgctcagtac tggaaatttt ccccagagca gcaagacgtt gagtcaacgc gtgaatggcc
    14761 tcttcacggc tggtaaagcg cgcattcaga cacaacgcat cgcggtgggt cagagtcgtc
    14821 aggttcatca tgatcctcac ttgatcgttt ttatagtcgc gccagtgtgc ccgacaaaac
    14881 aataccattg cgtaatcaat ttcacttgat tgaaattatt tgcattttat ttgtattgtt
    14941 tacaggatga atttatcggg cataatggct aaattcgtat taatgagatg ctaaataatg
    15001 ggacacaaac ccttataccg acagattgct gatcgcattc gtgagcaaat tgcgcgtggc
    15061 gagcttaaac ctggcgatgc gttacctacc gaatcggcat tacaaacaga gtttggcgtc
    15121 agccgggtta cggtgcgtca ggctttgcgc cagttagtcg agcaacagat cctcgaaagc
    15181 attcagggca gcgggactta cgtcaaagaa gagcgggtca attacgatat ttttcagtta
    15241 accagttttg atgaaaaact gtcggaccgt cacgtcgata cacacagtga agttctgata
    15301 tttgaagtga ttccggctga cgattttctt cagcaacagc tacaaatcac cgtgcaggat
    15361 cgtgtgtggc atgtgaagcg cgtgcgctat cgcaagcaaa agccaatggc gctggaagaa
    15421 acctggatgc cgcttgcgtt gttcccggat ctcacctggc aggtcatgga aaattcgaaa
    15481 tatcacttta ttgaagaagt gaagaagatg gttatcgatc gtagcgaaca ggaaatcatt
    15541 cctctgatgc caaccgaaga gatgagccgt ctgcttaata tcagccagac aaagccgatt
    15601 cttgaaaaag tatcgcgtgg atatttagtt gatggtcggg tgtttgaata tagccgcaac
    15661 gcttttaata ccgatgacta taaatttacc ctgatagcgc agcgaaaatc atcgcgataa
    15721 gcacaaaaag gccatcatac gatggccaac catgtcgaaa ccgacagccc ttcgccattc
    15781 ctgtagaggg aagcggcgag ggctatttct tattacagat atttatttca gaacagtttt
    15841 cagtgcttca ccgatatccg ccaggctgcg aacggttttc acgcctgcgg cttccagagc
    15901 agcgaatttc tcatccgcag tccctttccc accggcaatg atcgcacccg cgtggcccat
    15961 acgtttgcct ttcggcgcag tcacaccagc gatgtaaccc acaactggct tggtaacatg
    16021 ctctttgatg tacgctgccg cttcttcttc agcgctaccg ccgatctcac cgatcatcac
    16081 gatcgcttcg gtctgcggat ctttttcgaa catttcgaga atgtcgataa agttagagcc
    16141 agggatcggg tcaccgccga taccgacaca ggtcgactga ccgaaaccgt aatccgtggt
    16201 ctgtttaacc gcttcatagg tcagtgtacc ggaacgggaa acgataccca ctttacccgg
    16261 tttgtgaatg tgacccggct ggataccaat tttgcattca cccggggtga taacgcctgg
    16321 gcagttcggg ccgatcatac gaacgcctgc ttcgtccagt ttcactttca cggtcagcat
    16381 atccagcgtc gggatgcctt cagtgatggt gataatcagt ttgatgcctg catcgatagc
    16441 ttccagaatg gagtctttgc agaacggtgc tggtacgtaa ataacagacg cggaagcgcc
    16501 agtagcagca acagcttcac gcacggtgtt gaacaccggc aggccgaggt gggtagtgcc
    16561 gcctttacct ggagttacgc caccaaccat tttagtgccg tatgcaatgg cctgttctga
    16621 gtggaaagtc ccctggctac cggtaaagcc ctggcagata accttggtgt ttttatcaat
    16681 taaaatggac attatttccc ctccactgcg gcaacaacct gctgagctgc atccgtcaga
    16741 ccttttgctg caataatatt caggccgctg tcagccagtt tcttcgcgcc gagttcggcg
    16801 ttgttacctt ccagacgtac cacgaccggt acgttaacac ccacttccgc taccgcgccg
    16861 atgataccgt cagcgatcag gtcgcaacga acgataccgc cgaagatgtt aaccagaacg
    16921 gctttcactt tgtcgtcaga gaggatgatt ttgaacgctt cggttacacg ttctttggtt
    16981 gcgccgccgc caacgtccag gaagttagcc ggttcgccgc cgtgcagttt aactatgtcc
    17041 atcgtaccca tcgccagacc tgcgccgtta accatacaac cgatgttacc gtccagcgca
    17101 acgtagttca gttcccactg tgcagcctgt gcttcacgcg gatcttcctg cgactggtca
    17161 cgcatttcgc gcagatcagg ctggcggaac agggcgttgc catcagcgcc cagtttgccg
    17221 tcgaggcaaa tcagatcgcc ctgtttggtg atgaccagcg ggttgatttc gatcagcgcc
    17281 aggtcgcgct ccaggaaaat ggtcgccagg cccatgaaga ttttggtgaa ctgctgaacc
    17341 agtttacctt ccagacccag tttgaacgcc agctcgcgtc cctgatacgg catcgggcca
    17401 gtcagcggat caagcgcaac tttatggatc aggtgcggag tttcttccgc cactttttcg
    17461 atttccacgc cgccttcggt ggaggccata aagaccacgc gacgggaact acggtctaca
    17521 acggcaccga gatacagctc tttagcgata tcggtcgccg cttcaaccag aatctggtta
    17581 accggttggc cattggcatc tgtttgatac gttaccagac gcttgcccag ccagttttct
    17641 gcaaaagcac ggatgtcttc tttgctgttt acaactttca caccgcccgc tttaccgcgg
    17701 ccaccagcgt gaacctgaca tttcactacc cacggaccgg caccgatttt tgaagcggct
    17761 tcttctgctt cgcgcggagt agtacaggca taacccaccg gtgctggtaa gccatagcgg
    17821 gcaaacagtt gttttgcctg atattcatgt aagttcatgt gttctgtcca tccttcagta
    17881 atcgttatct tttaaaccgt agaccgacac cctatccacg tcggtctaca gtgcaggtga
    17941 agcttaaact actacacgtc cagcagcaga cgcgtcggct cttccagtaa ctctttgatc
    18001 gttaccagga agcccacgga ttcgcgacca tcgatcagac ggtgatcgta ggacagcgcc
    18061 aggtacatca tcggcaggat ctcaacctga ccattcaccg ccatcggacg atctttgata
    18121 gcgtgcatac ccagaattgc gctctgcggc gggttgatga tcggcgtaga catcagggaa
    18181 ccgaacacac caccgttggt gatggtgaag ttaccaccgg tcagatcttc aaccgtcagc
    18241 ttgccatcac ggcctttgac tgccagctct ttgattttct tctcgatgtc tgccatgccg
    18301 agggtatcga catcacgcag aaccggcgtc accaggccgc gcggcgtaga aaccgccatg
    18361 ctgacgtcga aatagttgtg gtaaaccacg tcatcgccgt cgatagacgc gttcacttcc
    18421 gggtaacgtt tcagggcttc aaccaccgct ttcacgtaga aggacataaa gcccagacgg
    18481 atgccgtggc gtttttcaaa cgcttcaccg tactgcttac gcagatccat aatcggcttc
    18541 atgttgactt cgttgaacgt ggtcagcatg gcggtggagt ttttcgcttc cagcagacgc
    18601 tctgccacac gcttacgcag gcgagtcatc gggacacgtt tttcactacg tgcagccaga
    18661 gccggttgcg ccgccggagc agccgctgcc ggagcagact ctttcgccgg ggctttcgcc
    18721 agatgttttt ccacatcttc acgagtcaga cgaccaccca caccggtgcc tttaatggcg
    18781 ctggcgtcga gattgtgttc agccagcagg cgacggatcg ccgggcttaa cgcatcgttg
    18841 ttttgctctt ccagagacgc ctgctggcgt tgcgccggag tggacgcttt ctcttcagat
    18901 ttggcgctgg tttctttacc ggcgctgttg ccttcacgca ggcgaccaag gatctgacga
    18961 gacgttaccg ttgtaccttc atcttccaga accgcatcca gaatgccgtc tgctgatgcc
    19021 ggtacttcca gtaccacttt gtcagtttcg atttctacca gcacttcatc acgtacgact
    19081 gcgtcgccgg gttttttatg ccaggttgcg acggtggcat cggctacgga ttcaggcagg
    19141 tcagggacca gaatatctac gctactcatt gtgtatcctt tatttattcg acgttcagcg
    19201 cgtcattaac cagatcttgt tgctgtttct ggtgaacgga catataccct accgccggag
    19261 aggcggaggc cgggcggcct gcataacgca gagaagcccc aaacggaatc acttcacgga
    19321 aatgatgctg gctgcagtac catgcgccct ggttgagcgg ctcttcctgg caccagacaa
    19381 aatccttgac gtgagcaaac tgctgcaaca cttcctgcat cgctttatgc gggaacgggt
    19441 agagttgctc gatacgcaca atggcgacat cgtgttgatt gttcttacga cgctgttcca
    19501 gcaggtcgta ataaacctta ccagaacaca tcactacgcg cttcacgccc ttcggatcaa
    19561 gctcgtcgat ttcaccgatg gctggcagga aggtgccgtt cgccagttct tcgaggctgg
    19621 aaaccgccag cggatgacgc agcagggatt tcggcgacat cacgaccagc ggacgacgca
    19681 tcccgcgcag cgcctgacga cgcagcatgt ggtaaacctg tgccggggta gacggtacgc
    19741 aaacctgcat gttttgctca gcacaaagtt gcagataacg ttccagacgc gcggaggagt
    19801 gctccggccc ctgcccttcg taaccgtgcg gcagcaacat caccagacca cacatccggc
    19861 cccatttctg ctcgccagag gagatgaact ggtcgataac cacctgcgca ccgttggcga
    19921 agtcaccgaa ctgcgcttcc cagatggtca gggtgcgtgg ttctgcggtg gcataaccat
    19981 attcaaacgc cagcacggct tcttctgaca gtacggagtc ccagacacgg aacgcgccct
    20041 gaccgttatg gatgtgttgc agcggcgtgt aagtggaacc gttagactgg ttgtggatca
    20101 ccgcgtggcg gtggaagaag gtaccgcgac cggagtcttc acccgacagg cgaaccggaa
    20161 tgccttcatc aaccagcgtg gcgtaagcga ggttttccgc accgccccag tcgaacagtt
    20221 tctcaccggc tgccatcgcc tggcgatcgc cataaatctt ggcaacgcga gactgcattt
    20281 caactgcttc tggtaccgta ctgatacgtt tagccagttc ctgcaggcgc ttcatctcaa
    20341 ctttgttcgg gtactcttcg tcccattcgt ggttgaggta cggcgaccag gtgaaagagt
    20401 gcatgttcat cggacgccac tctgccacta cgcaatcgcc agcatccagc gcatcgcggt
    20461 acaggttaac catctcggtg gcatcttcca gcgtcgccac tttttcctgc tccagcttgt
    20521 cagcgtagat tttgcgcggt gtcggatgtt ttttgatttt ctgatacatc agtggctggg
    20581 ttgcgctcgg ctcgtcggct tcgttgtggc cgtgacggcg gtagcatacc aggtcgatga
    20641 agacatcacg tttaaaggtg ttacggaaat cgagcgccag acgggtcaca aaggcaacgg
    20701 cttccggatc atccgcgtta acgtggaaaa tcggtgcctg aaccatctta ccgatatcag
    20761 tacagtacgg tgtagaacgg gcatccagcg ggttagaggt ggtgaaacca acctggttgt
    20821 tgataacgat acgtaccgta ccgccaactt cataaccacg cgctttcgac atgttcagag
    20881 tttcctgaac cacgccctgc ccggtcactg cggcgtcacc gtggatggtg attggcagca
    20941 ctttgttgct gttcggctca tcaagtctgt ccagacgggc acgaacagag ccgataacga
    21001 ccgggcttac aatctcaagg tgagacgggt taaacgccag cgccaggtgc accaggccgc
    21061 catcggtctg gaagtcagac gagaagccca tgtggtattt cacgtcgcct gtgccgaggt
    21121 gttctttatg tttaccggcg aactcgtcga acaagtcttg cggtttttta cccagcacgt
    21181 tcaccagcac gttcagacga ccacggtgcg ccatcccgag aaccacttcg cgggtgccgc
    21241 tgttgccagc gtggcggatc atctctttaa gcatcgggat taacgcgtca ccgccttcca
    21301 gcgagaagcg ttttgcgcca gggaattttg cgccgaggta acgctcaagg ccttcagcgg
    21361 cggtcagttc gcttaagaag cgttttttct cttcgctatt gaaagtcgcg cgaccagact
    21421 cgatacgctg ttggatccag cgtttttctt cggtgctggt gatgtgcata tactcggcac
    21481 caatcgggcc gcagtaggtt tgcttgaggg cggaaataag ctcgccgagt ttcatggttt
    21541 ctttgccgct ggcaaatgaa ccgacgttga aggtttcctg gaagtctgct tcggtcagat
    21601 cgtggaaaga cggatccaga tcggccactt tatcttgctg ccacagtccc agcggatcta
    21661 gattcgcatg ctggtgacca cggaagcggt atgcgttaat gagctgcagg actttaacct
    21721 gcttcacatt ggtgtcaggg tcggagatcg ttgaagagta acgtgaagcg tctttcgcca
    21781 ggcggcggaa atattcacgc gtttgagagt ggaattgatc cggtttgact cccgtaccag
    21841 gtaactgctg gaacgtcgaa cgccagttag cgtcaaccga gtcaggatcg gttaagaagt
    21901 cttcatagag ctgttctatc cagctctggt ttgcgccaga gaggtaagaa gagtccaacc
    21961 aggctttcaa agcgctgttc tgcatcgtga tcccttaagc atctttttta tgcttacttc
    22021 gccgtggata ctaccacgca cagtgatgtg cgtgccgggg ttcacttgcg agctcttgtc
    22081 tacgtagtgg ctcgcgaagg aacctttaaa aactgtctta gtgtcagggg cagtttttaa
    22141 agatttcctg catttctttg ttacgcctga tgcgctgcgc ttatcaggcc tgcgggtaat
    22201 gacttgtagg ccggataagg cgttcacgcc gcatccggca ctggttgcct gatgcaacgc
    22261 ttgcgcgtct tatcaggcct acagtttacg cattacgttg caacaacatc gacttgatat
    22321 ggccgatggc gcgcgtcggg ttcagcccct tcggacatac actgacgcag ttcatgatgc
    22381 tgtgacagcg gaatacgctg aatgcatcgc tcaaaccgtc gaggcggctg tcagtctcgg
    22441 tatcacggct atcgatcagg aaacgatatg ccgctaacaa gcctgccggg ccgataaact
    22501 tatcgggatt ccaccagaaa gacggacaag aggttgaaca acatgcgcag agaatacatt
    22561 catacagccc gtcgagtttt tcgcgctgct ctggcatctg taaatgctcg cgagctggcg
    22621 gattttgtcc attattcaac aggtaaggct taattttctc atattgcgca tagaattgtc
    22681 ccatgtctac caccaaatcg cggatcaccg gtaaacctgg cagcgggcga atcacaatct
    22741 tcttgcccgg ctggttgagt gccgaaatcg gggtaataca ggccagacca ttcttgccgt
    22801 tcatgttcag accgtcggaa ccgcacacac cttcacggca ggagcggcgg aacgacaggc
    22861 tgggatcttt ctctttcagc tggataagcg catccagcag catcatgtcg cgaccttctt
    22921 ccgcttccag ggtgtaatcc tgcatacgcg gagcatcatc aacatccggg ttatagcgat
    22981 aaattgaaaa ctcgagtctc attttcctgt ctccgcatta gtaagtacga atcttcggcg
    23041 ggaatgccgg gcgcagtttc ggttccatgt tgacgcttcg gcgcgtcatg gattccgact
    23101 caggcagata cagggagtgg cacagccagt tttcatcatc acgatccggg aagtcgaagc
    23161 ggctatgtgc gccacggctt tcggtacgga agttggcaga aacagccgtt gcatacgccg
    23221 tttccatcag gttatccagt tccaggcact caacgcgctg ggtgttgaac tcgctggaag
    23281 tgtcatccag acgggcattt ttcagacgct cgcggatcac tttcaactgc tcaagccctt
    23341 tcgccatcgc atcaccttca cggaagaccg agaagttatg ctgcatacat tcttgcagcg
    23401 ctttacggat cgccaccgga tcttcaccgt tacgattatt gttccaccgg ttcaggcgat
    23461 ccagagacgc ttcaacatca gactcgctgg catcgtgcag tgcgccctgc tcggcgatag
    23521 actcttgcag atgcagacct gccgcgcgac caaagaccac caggtccagc agcgagttgc
    23581 cgcccagacg gttagcgccg tgtaccgata cacaagcgat ttcaccaacg gcaaacagtc
    23641 ccggaacaac cacatcttcg cctttctcat tcacagtcag cgcctgaccg gtaactttgg
    23701 tcggaatacc gcccatcatg tagtgacagg ttgggataac cggaatcggc tctttcaccg
    23761 gatcgacgtg agcgaaggta cgggaaagct ccaggatacc cggcagacgg gattcgagaa
    23821 cttctttacc caggtgatcg agtttcagtt tcgcgtgtgg cccccacgga ccatcacagc
    23881 cgcgaccttc acggatttca atcatgatgg aacgcgccac cacatcacgg cccgccaggt
    23941 ctttggcgtt cggcgcataa cgctccataa agcgttcgcc atgtttgttc agcagataac
    24001 cgccttcacc acggcaacct tcggtgacca gtacgcccgc accggcaatg ccggtcgggt
    24061 ggaattgcca catttccata tcctgcaccg gtacgcctgc gcggatagcc atgccgacac
    24121 cgtcgccagt gttaatgtgg gcgttagtgg tggactgata aatacgccct gctccgccag
    24181 tcgccagcac ggtagcgcgg gctttgaaat aaaccacttc accggtttcg atgcacagtg
    24241 cggtacaacc caccaccgcg ccatcctggt ttttcaccag atccagcgca taccactcgg
    24301 agaaaatggt ggtgtggttt ttcaggttct gctgataaag cgtgtgcaac agtgcgtgac
    24361 cggtacggtc agcagccgcc gcagtgcgtg ccgcctgctc gccgccgaag tttttcgact
    24421 gaccgccaaa cggacgttga tagatacgac catcatcgag acgcgagaac ggcaggccca
    24481 tgtgttcgag ttccagaatc gcttccggcc cggttttaca catatattca atcgcgtcct
    24541 ggtcaccgat atagtccgac ccttttacgg tgtcgtacat atgccattcc cagttatctt
    24601 catgggtatt acccagcgca acggtaatgc cgccttgcgc agaaacggta tgggaacggg
    24661 tcgggaagac tttagagagc agggcacagg tctggccgct ctgggaaatt tgcagcgccg
    24721 cgcgcatacc tgcgccaccg gcaccaatca caactgcatc aaattctctg actggcaatt
    24781 tcatcacaca ccccacacca caacgaatcc ataaatcacg taaaccacca gtgcaacgac
    24841 aatcaccagt tgcagcatca ggcgcaaggc cagcggttta acgtagtcgg tcaacacctg
    24901 ccacatgccg atccaggcat gaatcaagat agaaaacagc gccagcaggg tgaacacttt
    24961 ggtgaacgca gaggcgaaga aaccgatcca gacttcatat gtcagttcgc cactggtggc
    25021 gaaaaaaccg accatataaa tgatgtagag cgtcaggacg atagcggtag cgcgaacgag
    25081 gatgaaatca tgtacgccat tgcgtcctaa tgcggaggcg ttgcttacca tacgaggact
    25141 cctgcgagaa gtgaaagcac gacagtaata acaaaggaga ttttggcgga gcgtttaccc
    25201 gcttcgaatg tttcttccag atagccaaaa tccatcatca tgtggcgaat acctacgacg
    25261 acgtgatacg ccagagcggt aaggatgccc cacatgataa atttgacgaa gaagctgccc
    25321 ataatcgcgg aagcttgctc gaaaccttca ggggaagaga ggctggtacc cagaagccac
    25381 tgcaggatgc ccactgcaac aaaggtgatc acaccggaaa cgcgatggag aatggacgct
    25441 atcgccgtga tggggaaccg gatggtctgt aggtccagat taacaggtct ttgttttttc
    25501 acatttctta tcatgaataa cgcccacatg ctgttcttat tattccctgg ggactacggg
    25561 cacagaggtt aactttctgt tacctggaga cgtcgggatt tccttcctcc ggtctgcttg
    25621 cgggtcagac agcgtccttt ccataactgc gcgtcatgca aaacactgct tccagatgcg
    25681 aaaacgacac gttacaacgc tgggtggctc gggattgcag ggtgttccgg agacctggcg
    25741 gcagtatagg ctgttcacaa aatcattaca attaacctac atatagtttg tcgggtttta
    25801 tcctgaacag tgatccaggt cacgataaca acatttattt aatttttaat catctaattt
    25861 gacaatcatt caacaaagtt gttacaaaca ttaccaggaa aagcatataa tgcgtaaaag
    25921 ttatgaagtc ggtatttcac ctaagattaa cttatgtaac agtgtggaag tattgaccaa
    25981 ttcattcggg acagttatta gtggtagaca agtttaataa ttcggattgc taagtacttg
    26041 attcgccatt tattcgtcat caatggattc tttacctgca agcgcccaga gctctgtacc
    26101 caggttttcc cctctttcac agagcggcga gccaaataac aaacgggtaa agccaggttg
    26161 atgtgcgaag gcaaatttaa gttccggcag tcttacgcaa taaggcgcta aggagacctt
    26221 aaatggctga tacaaaagca aaactcaacc tcaacgggga cacagctgtt gaactggatg
    26281 tgctgaaagg cacgctgggt caagatgtta ttgatatccg tactctcggt tcaaaaggtg
    26341 tgttcacctt tgacccaggc ttcacttcaa ccgcatcctg cgaatctaaa attactttta
    26401 ttgatggtga tgaaggtatt ttgctgcacc gcggtttccc gatcgatcag ctggcgaccg
    26461 attctaacta cctagaagtt tgttacatcc tgctgaatgg tgaaaaaccg actcaggaac
    26521 agtatgacga atttaaaact acggtgaccc gtcataccat gatccacgag cagattaccc
    26581 gtctgttcca cgctttccgt cgcgactcac atccaatggc agtcatgtgt ggtattaccg
    26641 gcgcgctggc ggcgttctat cacgactcgc tggatgttaa caatcctcgt catcgtgaaa
    26701 ttgccgcgtt ccgcctgctg tcgaaaatgc cgaccatggc cgcgatgtgt tacaagtatt
    26761 ccattggtca gccatttgtt tatccgcgca acgatctctc ctatgccggt aacttcctga
    26821 atatgatgtt ctccacgccg tgcgaaccgt atgaagttaa tccgattctg gaacgtgcta
    26881 tggaccgtat tctgatcctg cacgctgacc atgaacagaa cgcctctacc tccaccgtgc
    26941 gtaccgctgg ctcttcgggt gcgaacccgt ttgcctgtat cgcagcaggt attgcttcac
    27001 tgtggggacc tgcgcacggt ggtgctaacg aagcggcgct gaaaatgctg gaagaaatta
    27061 gctccgttaa acacattccg gaatttgttc gtcgtgcgaa agataaaaat gattctttcc
    27121 gcctgatggg cttcggtcac cgcgtgtaca aaaattacga cccgcgcgcc accgtaatgc
    27181 gtgaaacctg ccatgaagtt ctgaaagagc tgggcaccaa agatgacctg ctggaagtgg
    27241 ctatggagct ggaaaacatc gcgctgaacg acccgtactt tatcgagaag aaactgtacc
    27301 cgaacgtcga tttctactct ggtatcatcc tgaaagcgat gggtattccg tcttccatgt
    27361 tcaccgtcat tttcgcaatg gcacgtaccg ttggctggat cgcccactgg agcgaaatgc
    27421 acagttacgg tatgaagatt gcccgtccgc gtcagctgta tacaggatat gaaaaacgcg
    27481 actttaaaag cgatatcaag cgttaatggt tgattgctaa gttgtaaata ttttaacccg
    27541 ccgttcaaat ggcgggttaa tttttatatg cctaaacaca aaaaattgta aaaataaaat
    27601 ccactatcgg acattcatat attctaaaaa aaaatacatc ggtataaatt ataaacaaat
    27661 agatgatttt taatcatcta tcttcctctc agttacatga ataaaccacc aaatatagcg
    27721 cacccctata attcccaata ctttattcat caaacattta attcatacca tcaattaatt
    27781 acttttacat ttaataaaat taataatatt tttaagaaaa actaaaaaca ttcgacaatc
    27841 aaataagata ttcttgcacc gatatacttt tttcttttct gttaaaggac tcagttatgt
    27901 ttaaaggaca aaaaacattg gccgcactgg ccgtatctct gctgttcaca gcacctgttt
    27961 atgcggctga tgaaggatcc ggtgaaattc actttaaagg tgaagttatt gaagcaccgt
    28021 gtgaaataca tcaggatgat attgataaag aggttgaact cggtcaggtg accaccagcc
    28081 acattaatca gtcacatcac agcgatgccg ttgctgtcga cctgctctta gtcaactgtg
    28141 atctggaaaa ctccagcaac ggttccggtg gcaagatttc taaagttgca gtcacctttg
    28201 atagctcagc gaaaaccacc ggcgcagatc cgattctcaa caacaccagc acgggtgaag
    28261 ccaccggcgt cggcgtacgt ttaatgaata aagatcaaag caatatcgtt ttaggcaccg
    28321 ctactccaga tatcgacctg gctccgacct ccagcgaaca aacgctgaat ttctttgcct
    28381 ggatggaaca aattgatcag gctacacctg taacgccagg cgcagttacg gcgaatgcaa
    28441 cctacgtgct ggattataaa taagtattac tttggcggcg ctatcgccgc ctttcataaa
    28501 atatttatca ggtatggaca ccgtgaatat ttatcgactc tcttttgtat cctgcctggt
    28561 cgtggcgatg ccttgcgcat tggcggtcga attcaatctg aatgttctcg ataaatcaat
    28621 gcgcgaccgc attgatattt cattattaaa ggaaaaagga gtcattgctc ccggtgagta
    28681 ttttgttagc gttgcggtaa ataataacca aatcagtaac gagcaaaaga ttaactggca
    28741 caaaaatgac gataaaacca ttccgtgcat caatgattta ctggtcgata aatttggctt
    28801 aaaacctgaa gtccgtcagt cgttaccatt gataaatcag tgcgtcgatt ttagctcccg
    28861 acctgaaatg ctcttcaatt tcgatcaagc caatcagcaa ctgaatatta gtattccgca
    28921 agcctggctg gcgtggcact cagaaaactg gaccccaccc tccacatgga aagaaggtgt
    28981 cgccggtgtc ctgatggatt acaacttgtt tgccagcagc taccgcccac aggatggcag
    29041 cagcagcact aatcttaacg cctatggaac agccggaatc aacacggggg catggcgctt
    29101 acgcagtgat tatcagttga atcatactga tagcgatgat aaccatgaac agtcaggcga
    29161 aatatcacgt acctatcttt ttcgcccgtt accgcaatta ggttcaaaat taaccctcgg
    29221 cgaaacggat tttagtccca atattttcga cggtttttct tataccggcg cggcactggc
    29281 aagtgacgat cgaatgttgc catgggaact acgcggctac gccccacaaa ttagcggtat
    29341 tgcacagacc aatgccacgg tgacgatcag tcaatcaggc cgcgtcattt accagaaaaa
    29401 agtcccacca ggcccattta tcattgacga ccttaatcag tctgttcagg gcacactgga
    29461 tgtcaaagtg acggaagaag atggtcgggt gaacaatttc caggtttcag cagcatcgac
    29521 gcccttcctg actcgtcagg gacaggttcg ctataaactg gccgcgggtc agccacggcc
    29581 ctccatgtca catcaaactg aaaatgaaac cttttttagc aatgaagttt cctgggggat
    29641 gctgtcaaac acctcgctgt acagcggcct gctgctttct ggtgatgact accattctgc
    29701 cgcaatgggt attgggcaaa atatgctgtg gcttggtgcg ctgtcgtttg atgtcacgtg
    29761 ggccagtagc cattttgata ctcagcagga cgagcggggc ttaagctacc gttttaatta
    29821 cagcaaacaa gtggatgcca ctaacagcac gatttccctc gccgcttatc gtttctccga
    29881 tcgtcatttt cacagctacg ccaactatct ggatcacaaa tacaacgaca gcgatgcgca
    29941 ggacgaaaaa cagacgatca gcttatctgt gggccaaccg attaccccac taaacctcaa
    30001 tctttacgcc aacctgctac atcaaacctg gtggaatgca gacgcctcca cgaccgccaa
    30061 catcacggca ggttttaatg ttgatattgg tgactggaga gatatctcta tttctacgtc
    30121 attcaataca acccattacg aagataaaga tcgcgacaac cagatttacc tgtcgatttc
    30181 gctccccttc ggtaacggtg gtcgggttgg ctatgacatg caaaacagta gccacagcac
    30241 cacacaccgc atgtcgtgga acgatacgct ggatgaacgt aatagctggg gcatgtctgc
    30301 cggactgcaa tccgaccgtc ctgacaatgg agcccaggtg agcggtaact atcagcacct
    30361 gagttcagcg ggtgagtggg atatttctgg tacctatgcc gccaatgatt acagttccgt
    30421 cagcagcagc tggagcggtt ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg
    30481 cagctccacc aatgaaccac gcctgatggt cagcaccgat ggcgtggcag atattccggt
    30541 tcagggcaat ctcgactaca ccaaccattt tggcattgcg gtggtgccgt tgatttccag
    30601 ttaccagcct tccaccgtgg cggtgaacat gaatgactta cccgacggcg taacagttgc
    30661 agaaaacgtt atcaaagaaa cgtggattga aggcgcgata ggttacaaat cactggcttc
    30721 ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct
    30781 cggagcggat atccgccagg atgacagcgg cattagcgtg gggatggttg gcgaggaagg
    30841 acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag
    30901 ccagcattgc tcgctccatc ttcctgaaca tatggaagac accgcaaatc gcctgatttt
    30961 accttgtcat taattaagga aatgaaaatg acatttatga aaggactacc ccttttgttg
    31021 ttggttgcca gtctgtgcag ccacgctgca ctacaacccg atcgcactcg tattgtgttt
    31081 aacgctaatg ataaagctac cagtctgcgg gtggataatc gtagcgataa actcccctac
    31141 ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc
    31201 ctgccgccga tacaacgtct ggaaccgaaa gcaacaacac aggtacggat agtaaagcaa
    31261 gcctcgacca caaaattacc gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa
    31321 attcctcccg ccccggaaaa aaacagcgac catgccgttc tgcaagtcgc cattcagagt
    31381 cgtatcaagg tgttctggcg accggctgca ttacgcaaga aagcaggaga aaaggtcgaa
    31441 ctgcagttac aggtcagcca gcagggcaac caactgacac tgaaaaatcc taccgcgtat
    31501 tatctgacca ttgcctacct gggacgaaat gaaaaaggcg ttctccccgg cttcaaaacc
    31561 gtgatggttg cgccttttag tacggtcaac acaaacaccg gaaattatag cggcagccag
    31621 ttttatctcg gttatatgga tgattacggc gcgttacgca tgacgacgct caactgcagc
    31681 ggacaatgcc atttacaagc cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc
    31741 tcgtcatctg tctgttattt ctgtcgttga agtctgcgct tgccctgaac tgctactttg
    31801 gttcatccgg tggctcggta gaaaaatcag aagcgattca accttttgct gtaccaggca
    31861 atgccaaact cggcgataag atctgggaat ctgacgatat taaaataaga tctgggaatc
    31921 tgacgatatt aaaattcccg tctattgtga caacaatacc aacggcaact ttgaaagcga
    31981 acatgtctac gcatgggtaa acccctaccc gggggtgcag gatcgttatt atcaactcgg
    32041 agtaacttac aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa
    32101 ccagtgtatc gacagtaaga acattgacat ttatacccct gatgggatgg cagaacaaaa
    32161 tttgctcggg tgatcccgct aatattcaca tgtcgcgaac ctttctcgct cgcatgcggt
    32221 tatacgtcaa aatacgagaa atgccgccgc atgattatca aagtacgctt agcgactata
    32281 tcgtcgtgca atttgacggt gccggtagcg ttaacgaaga ccctactgcc caaaacctga
    32341 aatatcatat tactggtctg gaaaacattc gcgtgctgga ttgcagcgtc aatttttcca
    32401 tttcaccgga aacacagatg attgattttg gtaaatttaa tttgctggat attcgtcgcc
    32461 acacaatgtc gaaaacattc agtattaaga cgacaaaatc acaaaatgac caatgcaccg
    32521 acggatttaa agtcagttcc tccttttata ccgaagaaac gttggttgaa gaagataaag
    32581 cattgttgat tggtaatggt ctgaagctac gtttgctgga tgaaaacgcc tcgccttata
    32641 ccttcaataa atatgctgaa tacgccgatt tcaccagcga catgttggtc tacgaaaaaa
    32701 cctatacagc tgaactttcg tccattgcag gtacccccat cgaggctggg cccttcgata
    32761 cagtggtgct ttttaaaatt aactataact gatttgccat gatgcccttt gcagccggaa
    32821 agggcatctc tttcagaaaa ttgctgattt tccccttttc cttgattgct gttatttttt
    32881 cctagcccgg ctttctttat gctgtatcga tacaccaaag agaaaataat gagggagcga
    32941 aggatgccag ttttgcagtg gggaatgtta tgtgtgctat cacttctcct ttctattggt
    33001 ttcctcgcgg tacatctccc ggcggcgcta ttacttgggc cgatgatcgc cgggatcatc
    33061 ttcagtatgc gcggaattac tctgcaactt ccccgctccg cttttctcgc cgcgcaggcc
    33121 attcttggct gcatgattgc gcaaaacctc actggttcga ttctcaccac gctggccgcc
    33181 aactggccga tcgtgcttgc tattttactg gtgacgctgc tttccagcgc catcgtgggt
    33241 tggttattgg tgcgctatag ctcactgcct ggaaataccg gtgcctgggg ttcttctcca
    33301 ggcggcgcag cggcaatggt cgctatggcg caagattacg gcgcagatat tcgcctggtg
    33361 gcgtttatgc aatatctgcg agtgctgttc gtcgcggggg ccgctgttct ggtcacccgc
    33421 atgatgctgg gtgataacgc tgaagcggtt aaccagcaaa tcgtctggtt cccgccagtg
    33481 agcattaatc tcctgcttac gattttgctc gcagtcgttg ccggtacggc gggatgcatg
    33541 ctgcgtcttc cttccggcac gatgctcatc ccgatgctgg cgggcgcagt gctccagtct
    33601 ggtcagctca tcaccatcga actaccggaa tggctgctgg cgatggcgta tatggcaatt
    33661 ggctggcgga ttggtcttgg tttcgataaa caaatcttac tgcgggcact acgcccgcta
    33721 ccgcaaatcc tgctgtcgat ttttgctctg ctggctattt gtgcaggtat ggcgtgggga
    33781 ctgacccggt ttatgcatat tgattttatg actgcctacc tcgccaccag tcccggcggg
    33841 cttgataccg tggcggtcat cgccgcaggg agcaatgccg atatggcgct tattatggcg
    33901 atgcaaaccc tgcgcctgtt cagtattttg ctgacggggc cagccattgc gcggtttatt
    33961 tcaacctatg cgccgaagcg gtcggcctag tgctggcagc ctggacacca gtaaaacggg
    34021 cgcgaggaca acgtggtttt ctcaatgatg ccgccacagc gttcgcacag ttcgccatca
    34081 cgatgaaaaa ccttaaagcg aaacagcgcc ccatgatgct tattctcatc cacctgcccc
    34141 cgcgtagcgt aagaaaaccg aggaatctcc agtaacgcgt gtgcgagtgc atccagttgc
    34201 gccgcattga gatctttcgc tttatgattt ccagtcaacc caacctgcca gaggatctcc
    34261 acccgcaaat aattgccaag cccagccaga aacgcctgat cgagcagtaa tcctgcaaac
    34321 tgacggttac gaaagcgcgg cgacaataac cgttccttta ccacctccgg cgtcagattc
    34381 ggatccagca catcgggacc aacgcgttgt aaaaacggat gcgtggtcag ttgttccggc
    34441 ctcaacatct caatatccga ggcgctataa agcagaatgg ttttgtcagc cgtttgcagt
    34501 tttacccgca atactcgcgt ggtctgcggc tcttcgccgg tatcaaccac gcgccagacg
    34561 ccgtaaagct gattatggct gtagagcgtt aaaccgttgg gaaaatgagt taacaacgcc
    34621 ttaccacgcg tttccacatg ggtaacgtgt tgaccgataa gttgtgattg ataagttttt
    34681 aactgcggga aggcaaacca gacatcagtt agtggtttgc ctttgatcgc cgcctccagg
    34741 ttatccgctg cacggcggat ctccgggcct tcaggcatgg tgatatcctt ttttcattct
    34801 ttattaagcg gggttatgct gcgacaacaa tccccttttt ggcaaatgta gctcgtaatc
    34861 ggcgggcgaa cgccagagcg tgctcgccgt cgccatgcag gcagaccgtt tgcgccgcga
    34921 ccgttgccca ttcgccggtg atacttttga ctctgccgtg ttgcaccatc tccagcgttt
    34981 gcgccaacgc ctgttcttcg ttttcaatca acgcgcctgg ctgacttcgc ggcaccagcg
    35041 agccgtcagc ctgataaccg cgatcggcaa acacttcctc gcgcgttgtc aggccatatt
    35101 gcttgcctgc acgaatcagc tcgcttcctg ccagcccaac gagaaccaat gccggatcgc
    35161 aagcgtatac cgctctggcg atggcgtctg ccagttgtgc ttctttcgcc gcctggttgt
    35221 acaacatgcc gtgcggtttg acatgacgca ttacgccgcc ttgcgcacgg gcaatcgttg
    35281 ccagcgcgcc aatttgatac agcgtctggg cgtaaacggt ttccggcggc agctgcatgg
    35341 cgctgcgacc aaaattttcc ctgtcgggaa aactcgggtg agcgccaatc gcgacaccat
    35401 tttttattgc ttcacgcacg caagcctgca tggtttgcgc atcgcctgca tgaaatccac
    35461 aagcaatatt ggcagaggaa accagcgtta atagctctgc gtcgctggcg cagccttcgc
    35521 ccagatcggc gttcaggtca attttcattg tgcagccgcc acgctaattg ttcaaaataa
    35581 cgttgctgat cttgccgcgc tttcagtgcc tcttccagtg aacactggac aaaatgaatc
    35641 ggctgaccga gcggaatttg cgccagatgg tacatatcag cctcaatgat acaggcaata
    35701 cgtggataac caccggtggt ctgtgcgtcg ttcatcaaca caatcggctg cccgttatgc
    35761 ggcacctgca ccacgctcgg taacaaaccg tgagataaca gttcgcgatc ggtgatgcgt
    35821 tttaaaattt gcccctgtaa acgatagccc attcggttac tttgcgagct aagctgccag
    35881 ggcgaacgcc agaatgcatc ctgcgaggcg cgatcgaact catgatattc cggccccggc
    35941 aaggcgcgaa tgcggttgcc ccacagcagc tgtttaacgc cctgcgcttc cataaaatca
    36001 cgcttcgatt tgccaatcgg gagtcagtca ccatccttca gtaaacggcc ttccagcccg
    36061 ccaatcccca ctttgagatc ggtgctgcat gaccccatta ccggcggaac atcaatacca
    36121 cccgcgaccg ccagataact gcgcatcccg tgctgcgggc gtttaagcgt taaacgctgg
    36181 cctgctttca tcggcaatcg ccagccggtc cagacggcgt tatcatccaa ccgcgcttcg
    36241 caacctgcac ccgtcagggc aaaccaccca tcagtttcga actcaactgt taactgaccg
    36301 agcgtgatct ccagcgcggg ggcattggcg tcattaccca ccagcaggtt agcaatgcgc
    36361 agcgcaggca tatccagtgc gccacagtgg ctgatacccg actggcaaaa accgtgacga
    36421 ccgccatcct gcacagtggt gtacatgccc gcacgaataa tcttcagcat attccctcct
    36481 tctgcggtac aaatcgcacg ctgtctcccg gacgtaataa gatgggttcg tcacgcgccg
    36541 gatcaaacag gctgagtgag gtatgaccaa tcaactgcca gccacccggc gttgccagcg
    36601 gataaacacc agtctgcggc ccgccgatcc cgacagaacc tgccggaacg agcaagcgcg
    36661 gttcagcgcg ccgtggcgtg tgtagttgtt ccggcaaact cccgagatac gggaagcccg
    36721 gttgaaaacc taaaaaccag accacgtatt ccacggagga gtgcaattca acaacctgtt
    36781 tttcgctcaa cccgcaatac gccgcgacca ccgccaaatc cggtccgcct gcaccaccgt
    36841 aaaccaccgg aatttcaata aagcgagact caggttccag ggcctcgctc tcctcccacc
    36901 agcgttgcaa acgctctatg gcatccagcg ccagcgactc aggattacgc aaaatcaccg
    36961 tgatattgtt catgccggga atggcttcaa ccacattcgg catatccacc agacgctgcg
    37021 ccagtcgcca gatccgtttc tggctagcca gcgtcaccgg cggttccagt tccagcacta
    37081 ccgccgtttc acctatcaga taacaacgcg ctcgttgcac tttcgatccc tctgattatt
    37141 cgttatgcag gattaggaat atcaataaag gtcacatcaa gatcggtatt ttcattcagc
    37201 cactcgctca atgcgcgaat accaccgcgt tcagtggcat ggtgacctgc agcataaaaa
    37261 tgcaatccct gctcgcgggc tgaatgaatg gtctgttcag aaacttcgcc agtaataaaa
    37321 gcatccacgc caaaacgcgc ggcgctatcg ataaaacttt gcccgccgcc cgtgcaccag
    37381 gcgacgcgct ggaccacctc aggtccggta tcgccacacc ataacggctt acgtcccaga
    37441 cgcgcttcaa tccaggaggc tagttccatt cccggcacag gcatggtcag ctctccccac
    37501 ggcaccaacg gctcaatttc gcccatgacc gtgatcccca gtaacgccgc cagctgtgcg
    37561 ttattgccca gctcaggatg tgcgtcaagc ggcaaatgcc agccatacag gttgatatca
    37621 ttcgccagca acgtttttaa acggttacgc ttcattccgc gaatgaccgg agactctcct
    37681 ttccagaagt agccatgatg cacaatgacc gcatctgcgc ccagacgcac tgcctcatcg
    37741 agcaaagcct ggctggcggt gacaccggta acaatttttt gcaccgtctc tttgccttcc
    37801 acctgcaaac cattcggcgc gtaatcacta atcgccgcgc tgttcagttt ttcgttaatc
    37861 agttgttcca gttcggtgtt tttcatcttc gcgccttccg tattctgata gctacaccgt
    37921 taacatagcg ttacatcgcc tgttcgtcga tagccaatct tttgacatat taagaaacaa
    37981 acccgacgcc aaaagacgca aattgtttat atcatcacgc ttcagctaat gtatatttat
    38041 gcaatcagac agcatattgc ataaataatc agatctgtat caaagtttta actttaagga
    38101 aacacaagca cgccgcgcaa ctattacgtg acgttgccgt caattgtgga attattatga
    38161 ataaacacgc atcacaaccg cgcgctattt actatgtcgt tgcgctgcaa atctgggaat
    38221 attttagctt ttacggcatg cgtgccctgc tgattctcta tctcaccaat caactaaaat
    38281 acaacgatac tcacgcctac gagttattta gcgcctactg ttcgctggtg tatgtcacgc
    38341 caatcctcgg tggctttttg gcggataaag ttctcggcaa tcgcatggcg gtgatgctgg
    38401 gggcgttgtt gatggcgatc ggtcatgtgg tgctgggtgc cagtgagatc catccgtcat
    38461 tcctctatct gtccctggcg attatcgtct gcggctatgg cctgtttaaa tctaacgtaa
    38521 gctgtctgct cggcgagctg tatgagccaa ccgatccgcg tcgtgatggc ggtttctcgc
    38581 tgatgtatgc ggcgggtaac gtgggatcta ttatcgcacc tatcgcctgt ggttacgccc
    38641 aggaagagta cagctgggcg atgggctttg gcctggcggc ggttggcatg atcgcgggtc
    38701 tggtcatttt cttatgtggc aatcgtcatt tcactcatac ccgcggcgtt aacaaaaaag
    38761 tactgcgtgc gacaaacttt ctcctgccga actggggatg gctgctggtt ctgctggtgg
    38821 caacgcctgc actgattacc gtgctgttct ggaaagagtg gtcggtatac gccttaattg
    38881 tcgcgactat tattggtctg ggtgtactgg caaaaattta tcgcaaagca gaaaaccaga
    38941 aacagcggaa ggagctgggg ctgattgtga cgctcacctt cttcagtatg ttgttctggg
    39001 ccttcgcaca acagggcggt agctcgatta gcctttatat cgaccgcttc gttaaccgcg
    39061 atatgtttgg ttataccgtt ccgaccgcga tgttccagtc gattaatgcc ttcgcagtta
    39121 tgctgtgcgg tgtgttcctg gcgtgggtgg ttaaagaaag cgtcgcaggt aatcgtaccg
    39181 tgcgcatctg gggaaaattt gctcttggcc ttggcctgat gagcgccgga ttctgcattc
    39241 tgaccttaag cgcccgctgg tccgcaatgt atggtcactc ttctctgcca ctgatggtgt
    39301 taggcctcgc ggtgatgggc tttgcggaac tgtttatcga cccggttgcc atgtcgcaaa
    39361 ttacgcgcat tgaaattccg ggcgtgaccg gcgtattaac cggcatctac atgctgcttt
    39421 ctggcgcgat cgcgaactat ctggcgggcg tgattgccga tcagacatcg caggcttcgt
    39481 ttgatgcttc cggggcgatc aactactcca tcaatgcata tattgaagta tttgatcaaa
    39541 ttacctgggg cgcactggcg tgtgtaggac tggtactgat gatttggctg tatcaggcgc
    39601 tgaaattcag aaaccgcgcg ctggcgctgg agtcataata ggccagcacc gatgcctgat
    39661 gcgacgcttg acgcgtctta tcaggcctac gtatttcctg caatttattg aatttgcaca
    39721 aatttgtagg ctggattagg cgttcacgcc gcatccggca tctgggctcg attgcctgac
    39781 gcgtctgtta tttccccttc cgcgccgcct catacgctgc caacgtttgt actctcgcct
    39841 ctttgtgctc gactatcggt tgcggataat ccagcttcac acctgctttc tgcgcccact
    39901 tccacggctc atgcaccgct ttccctggta catcgcgcag ctccggtaac catcgacgaa
    39961 taaactcgcc ctcacggtca aatttctcgc cctgagttat cggattgaaa atacgaaaat
    40021 acggcgcagc atcagtaccc gtagaagccg cccactgcca gccaccgtta ttagctgcca
    40081 aatcaccatc aatcagctgc gacatgaaat atcgctcgcc ttcgcgccag tcgatcaaca
    40141 aatctttcac cagaaaactg gctgtaatca tccgtagccg gttatgcatc cagccagtgc
    40201 tgttaagctg acgcatagcg gcatcaataa tcgggtatcc cgttttgcct ttctgccagg
    40261 cctgtaaatg tgcgggattg ctttgccact gcacacgatc cgtccaggca ataaatggac
    40321 gatgtttaca caacgagggg taatacgtca tcagatgacg gtagaactcg cgccagatca
    40381 gctcactaag ccagacacta ccggccccac cgtccagcgc ctgcggctgt tccgccaaca
    40441 agcgatgcaa acactggcga ggcgataacc cgcccgttgc caagctggcg gacaaacggc
    40501 tggtgccttc cactgccgga aaatctcgtt gttgctcata ctctccagca ccgttctcgc
    40561 aaaactggcg taattgcgca atcgccgctt tttcttccac cgggaaatgc gcagtatcga
    40621 aagactgacg aggataattc agcgtaatgg atggcgcggg ctttatcgat ccgctactac
    40681 gaacttttgg tgcagcgacg cactccggca tcccttcccg cagccgtttc agccaggcat
    40741 tcttaaaagg cgtaaagact ttgtacatct cgtgattgcc ggtcatcacc gcccccggcg
    40801 gcaggatcac gctgtcatca aatccttcac acaccacgtt acgcagcgct ctttcaacgt
    40861 ccacatcccg cgctcgctca ttcacctcat actgatagtt ataaaaaaga tgagtaacgc
    40921 tgttttccgc gcacacctgt ttaactattt cgacactggc gacaaagtca tccacttcac
    40981 ggaacaataa aggaatacct ttttccgcca gcgcgatttg tagcccattc agttgagcat
    41041 tgataagttc agcctgacgc ggcgacatgt tatgcgtctc ccactggcgt ggtgtagcga
    41101 tatacagcgc cagcacgcgt gcagacgaat tgcggcaggc ggcagccagt gcgagattat
    41161 cgtgcagacg taaatcctgg cgaaaccagg ccagatgggt agtcataaaa ctcctgaatg
    41221 gcgcaagtta atcacacgcg gccaaagttc gaaataacgc tgccctgaaa gccaggcgtc
    41281 aggataaagc gccatatagt gtttgatacg catcagcggc gcaagaagtg gttgctcgcc
    41341 acggcgataa ctgtcgataa gcgcagccag ctgctggcgt tctgtggaat caatatgcgg
    41401 gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga tcgcgcgggt tggcgggatg
    41461 agaaagcaaa acaattacgc gttggcggta ctggttgtaa aaatcatcca gattttgcca
    41521 ctgatgaata tcagccacaa acgggccaag tttgcggtac tcgggctgag aatgggccag
    41581 aaagacgagt ttatagcggc tgtgataatc gagaagcgcc ccgcgcgtta agccatgttc
    41641 tttcagtcga tgtaactcat gaagggcgaa gatacggatt agggtgctgt catcaaatcg
    41701 ttgtagattc attactgctc ctgaacggta tacaggagta agtgtaggcg ttggtaaatg
    41761 atgtttgtga agttaatccg ctagcagggc aagattacga aagcgcgctc cccgcaagca
    41821 actaatcaag gtagttaatc atggctgaac acgatattac gccagaagtc accatttcca
    41881 aaacacagcg ccgctataaa gtcggctatg tcagagcccg ccaccaggac cgcaaaaccg
    41941 gtatagcccg gtattattca caacatccca gcctgcacct taagggcgac tggctgaaag
    42001 aggccggatt tgataccgga cggggtgtca cggtgaagat ttcggagggg ggcctgacca
    42061 tcatcgctga cagtgacgag gtgcaggaac tgcgggatgg gatgtttagt gtgctgaacg
    42121 aaagttaaat attaaaaaag ctgggaagat ttaaaacctt cctagctttt tattctgtct
    42181 tattataatg gatactcatc atcttccgat gaatttataa attgctccaa gtttacatga
    42241 agagtactta acctagtttt taactcatta agtgaagcga taatttcata ttttccaaca
    42301 taatcatatg atggatcgta ttcattatct atgtcagaag ctaaaaatga tgatattcgt
    42361 agtttaaatt tagcttcatc tttattacat tcattggcat aaggtgaatg ccctatcata
    42421 gcatattcat ctaatctaaa aggataagat ggttttaatt catcgatcaa tgaaatttca
    42481 atccatgttc tatatccagg cattaatgga ctaatttctt tgagcaaaac ctttccactt
    42541 aataatgtat tgagattgtt cattattatg tctcccacgg gaaaaatata tgtctaacac
    42601 ttccaccatg ttgatgaact tgtaaatgtg gtaaatcacc atcaggattt cctagtccat
    42661 gacctgacac tctatatgaa ggttttgtta cagaaggatc ttttatatca tcccaatgat
    42721 atgaacctgc ttttgtttta tttccagtaa gatattccat taagttctta gtgctggggc
    42781 ctgactcacc tgtcatattt ttataattat gacctaaata tctatccata aacaattgtt
    42841 cagcgtcact tctagaccta acagtaactt tggtttcact actatctaat aaacgattcg
    42901 ctttttggtt actaacagca catggagtta atccaagtgg atcagcatat tttagaggat
    42961 taggtgcata actgtagaga tttaaaccgc ctctcaaccc tatcgggtca gttaccatga
    43021 accgcccgac atccggatca tagtacctgt gcagattgta gtgcagcccc gtttccctgt
    43081 cgaggtactg tccctggtag cgcaggttct gctccacccc gccgtggtcc ttctcctgta
    43141 tcgtgttacc ccagacctga taccccgtct cccacacgat tttcccgtcg ctgtccgtca
    43201 tctcttccgg cgcgccgttc acatccgtgt gaaaatacag tatccggttc gggccctctt
    43261 tgcccgcctt atccacccgc gccagcggtt cataactccc ctggtccctg tagatgtaca
    43321 ggctgcctgt tccctccggc ctgctctcct gcgccagccg cattccgtcc cataaaaatt
    43381 ttgtccggtt gtacggctta ccgtcagggc tgatgtgctg tttttccgtc cgcctcccca
    43441 gtgcatcgta cacataccgg tatgtccccc gcgctgtcgt cacttccacc atccggtgtt
    43501 ccgcatcata acggtaactc tgcgttccgc tccggccctc cttttccacc gcacggccaa
    43561 attcgtcata acggtagtgc acaccacggt aagacatcag ccggttgaac ggcaccacgc
    43621 tgttttctgc cgtggccctt tcccccgcgc gcctccccag caggtttccc gccgcgtcgt
    43681 actgccagct cgccagatac ccctcatcct ccgaacgaca cgccgtgatg cgccccgtgg
    43741 ggtcgtagaa gtaatccgtt tccccccgcc ggctgtgccg cgtcttcagc agttcatcct
    43801 cgccactgta gctgtactgc cggtccgtga acgtctccgg taccaccccg cgcatcccgc
    43861 tgtatgtctc cctgcgcttc agccgcccgc cggggtcgta aagctgccgc gtgctcagtc
    43921 gcccctggct tctgagcact tcacggtgca ggtggtcgcg cgtgaactcg ctgatgacct
    43981 cccggtccag atttatctgc tggagatggc cgctgccgta gtacagatgg cgctgcgttc
    44041 tgccgtccgg aaaggtggtg gcagtccggt tgcccagttt atcgtactgg tgggtcagga
    44101 tgccctgcgc cgtctcctcg ctcagcatcc gccccagtct gtcgtagctg aagcggataa
    44161 cttccggcgc gtcagtttcg tgccgctggc gacggatttc cagtagctgg tctgccgcat
    44221 catggacgta ttccgtacgg ccatcggctg tctcttttgc cgtgagccgg cccgccgcat
    44281 cacgcgccag ccggtggcgg atttcaccgc cccggtcccc ggtgaacacc acttccgtga
    44341 ccgcgttcag cgcgttgtag cggtacgact ggcggcagcc gtccgggttt atctgctccg
    44401 ccagacggtc cagcacatca tggcggaacc ggtagcgttc tccgttctcg ttcgtgagcg
    44461 tgaccagacg cccgtaagcg tcgtacccga atgccgtcct gcgccccatc gcgtccgtcc
    44521 ggctgcgcac ccggccctgc ccgtcccgct gccagcgcgt tatcctcccc atcgggctgg
    44581 tgtactttac cagcctgccc gccgcgtctg actggtaatt caccgtgcgg ccgtccgcca
    44641 gtatcacctc cgtcagatga ccgctgtcgt catacccgta gcgcgtgctg tggctctgcg
    44701 catccgtcat tctgataagc ctcgtgcgtt cgtcgtagaa ccacgccgtc tgcgagccgg
    44761 aacagtccgt gtgccgcatg agctgcccgt cttcattcca ctggaggtat ttattccccc
    44821 cgcgcgcatc cgttatctgc accacctagc cgtggtggtc atactgatac cgggtgttct
    44881 gctgcagcgg gtcggtcacg gcaagaaggt tgccgcgttc atcgtacaca cagcgccagg
    44941 tgtttcccgc cgcgtccacc tccgtttccg gctggtgcca caccgggtgc cactgcgcct
    45001 gctccacccg ccccagcggg tcgtgcgtct ccgtgatatg cccctgcagg tcatacacat
    45061 agcgccattt gccgccctga gggtcggtca tccccagcaa cagtcgttct tcgtcgctcc
    45121 agcggaatgt cgtcacctgt ccggcttcgt cctggtacgc cgtcacctga ttctgcgcat
    45181 cccactggtg ataacgtgtg cgccccagtc cgtccgtcac gcacagcctg cgctgcgcca
    45241 ggtcccacgc cagccggtac tgttcaccgc tgtccgtgtg gtgccccgtc acccgccatg
    45301 ccgcgtcatc cggaccttca taccagcccc actggtaata atacccttcg ccgcccggct
    45361 ggcggtgcag cgtcatgcag tggttttcgt cgtaagcaaa ctcacgcagc aggtgccccg
    45421 tgctgtcgta cacgccgttc aggtggccgg cctcgtcata gctgtaacga cgcagcagta
    45481 aaggtgcagt ctcagggaaa tgctgataaa tctgtgtcac ccgccgggga tgcgccgcca
    45541 gttcgtaata cagacggatg cacggacgct gctgttcgcc gctgatttcc acaatgcggc
    45601 cccggtcgtc gtagtacaga ttcagacagt tgctgttacg gtcgcccagc attttcagcc
    45661 gctggcgctg cgggtggtgc gggtcagcct caaacagaaa acactgcccg tcatcgctgc
    45721 tgataagcca gtgcccctgc tcaccacgcc ttaccgccag cccttcaccg gggctgtaaa
    45781 acccgccccc cggcagtacg gcttccatat ccaagcgtcg ccccatgccc gacacaaagg
    45841 tcatgcagtt ttcgtctgcg ctacccggtg tgcacacgag gctgacttta tacattacac
    45901 tccagccctg accaaacatt ccttccgtac gttcgtcacg actgctgtac acgcgctgcc
    45961 agtccagcgg aaagtggccc ggcagactga aatcgaagtc atcgtcatcg gccaggtatt
    46021 tggccccggt ggcggcattg accggatgcg gaagggcact ggtggccttc tgcgtcagca
    46081 tgctcatccc gacactggca gcaaaacagg cgattttaga aagcattttg cccgggcgaa
    46141 gaaacatcag cgccacggtt gccaccaggg ttatctgcga cctgccgctt ctgatatccc
    46201 tgaccaccag taacggaccg ccaatgcgca catccggtga cacaaacgcc ccgttccccg
    46261 gctcattcac caccctggcc tcgcaggtac agcgtgcccc gctgcgtgtc gccggctggc
    46321 tgttgatacg cacatcccgc gcgccttccg cgatatattc cgctgcgggc ggctcgtcat
    46381 cgcctttgaa cagattactg atgctgccaa taaccgcatt cactccctga aaggcggttt
    46441 ccagcatacc gcccggattc agcaggttaa gggccgttcc cagggggtca tccagaaagg
    46501 cggctttttt ttgtgcgaga aactgcgtca gtgaggaagg atgtttatgg cagtcgattt
    46561 tatcgtcagg ggtcggggtg gatcccgggg ccgcactggc cacagtgggc tgccatagtt
    46621 ccgacacaaa atgtgtcacg ccatccacca tttcagaacg cccttcttcc gtactgagct
    46681 ggtcgtgcag agcccgggac atcagcacag gccctgtcac cgggttaacc gccagggcta
    46741 tggcccccgc catttccaga tattccccgg ccacatcccg gaacgttctg gcatcctcct
    46801 gacgctgcgc ttcttcctgt tctgccttcg cctgcgcttc ctgcgcctct atgtcctgcc
    46861 gcgatgccgc agtcgccgct gcccgcgcgg caggtttgct gttgatccat gtgtcaccag
    46921 agccggtgag gatgtaacct tcaatctgag gcgggaaaag catatttgcg agggctgaac
    46981 aggcttcgct gaccttttct gtcacgccgg tgtacaccat cgccacaccc gccacaaaac
    47041 cggtcagcac gacggccgtg caccccatcg tgatccccac tgacgtcgcc agtaacgagg
    47101 ttgtgccgac aatgagggca tcaataacga catttgcggc cacctccagc accgccccca
    47161 gcacatctgc cataaacgac gtatgcagca gggcatcacc ctgtcttgcc gcccagaatg
    47221 cctcttccat gtcccgttcc ctgtatgatt aattcgtcct gacttcctgc cgcatcctga
    47281 agcgcagatt ctttttaatt tcagcccagc gcgtcatatc tgccggggtc agcggtgtgg
    47341 ttctggcgta ggtgaaggcc atcatgaccc gctcacccgg caatacaaat gccacctgac
    47401 actggtacac ctgctcgtta ccccggagaa actggctctg tatttccaaa ccgcctcgtt
    47461 gagataatat tccgtcataa tgtaaatagt ccgttttaat aaaggaatta tttattgaca
    47521 ggttctgttt ttttaatcat gaatgtaata cccatcaccg ctttacagca cactttatct
    47581 ttccagccat aatcactaat aatattaagc aattagcaat atttacaacg accagagcgg
    47641 atacatagtt atgtatttaa caatcccaat atattcacta tgcaaataaa accactagta
    47701 acagacacgc taccgcctcc atataaaaat ggacacctta taaaattgta tcttgcatag
    47761 aaaaagctga aatatcggat gcgttttaat tatcaactat aaatctataa gctttttatt
    47821 aacaa
//
LOCUS       DAJVRP010000023        47334 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVRP010000023 DAJVRP010000000
VERSION     DAJVRP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.023"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OQN12_001283"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OQN12_001283"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS1653678.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="OQN12_001284"
     CDS             complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="OQN12_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1653679.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="OQN12_001285"
     CDS             complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="OQN12_001285"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1653680.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="OQN12_001286"
     CDS             complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="OQN12_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1653681.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="OQN12_001287"
     CDS             complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="OQN12_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1653682.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="OQN12_001288"
     CDS             complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="OQN12_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1653683.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="OQN12_001289"
     CDS             complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="OQN12_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1653684.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="OQN12_001290"
     CDS             complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="OQN12_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1653685.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="OQN12_001291"
     CDS             complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="OQN12_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1653686.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="OQN12_001292"
     CDS             complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="OQN12_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1653687.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="OQN12_001293"
     CDS             complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="OQN12_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1653688.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="OQN12_001294"
     CDS             complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="OQN12_001294"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1653689.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10285..11088
                     /gene="suhB"
                     /locus_tag="OQN12_001295"
     CDS             10285..11088
                     /gene="suhB"
                     /locus_tag="OQN12_001295"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1653690.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11233..12087
                     /locus_tag="OQN12_001296"
     CDS             11233..12087
                     /locus_tag="OQN12_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1653691.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12278..13558
                     /gene="csiE"
                     /locus_tag="OQN12_001297"
     CDS             12278..13558
                     /gene="csiE"
                     /locus_tag="OQN12_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1653692.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13550..14689)
                     /locus_tag="OQN12_001298"
     CDS             complement(13550..14689)
                     /locus_tag="OQN12_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1653693.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="OQN12_001299"
     CDS             complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="OQN12_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1653694.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15875..17236
                     /locus_tag="OQN12_001300"
     CDS             15875..17236
                     /locus_tag="OQN12_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1653695.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17233..17751
                     /locus_tag="OQN12_001301"
     CDS             17233..17751
                     /locus_tag="OQN12_001301"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1653696.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17751..18071
                     /locus_tag="OQN12_001302"
     CDS             17751..18071
                     /locus_tag="OQN12_001302"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1653697.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18068..18880
                     /gene="hcaB"
                     /locus_tag="OQN12_001303"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18068..18880
                     /gene="hcaB"
                     /locus_tag="OQN12_001303"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1653698.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18890..20092
                     /locus_tag="OQN12_001304"
     CDS             18890..20092
                     /locus_tag="OQN12_001304"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCS1653699.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20189..20611
                     /locus_tag="OQN12_001305"
     CDS             20189..20611
                     /locus_tag="OQN12_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1653700.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20659..21531)
                     /locus_tag="OQN12_001306"
     CDS             complement(20659..21531)
                     /locus_tag="OQN12_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1653701.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21543..22637)
                     /locus_tag="OQN12_001307"
     CDS             complement(21543..22637)
                     /locus_tag="OQN12_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1653702.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22670..23668)
                     /locus_tag="OQN12_001308"
     CDS             complement(22670..23668)
                     /locus_tag="OQN12_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1653703.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23693..25204)
                     /locus_tag="OQN12_001309"
     CDS             complement(23693..25204)
                     /locus_tag="OQN12_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1653704.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25227..26210)
                     /locus_tag="OQN12_001310"
     CDS             complement(25227..26210)
                     /locus_tag="OQN12_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1653705.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26307..29408)
                     /locus_tag="OQN12_001311"
     CDS             complement(26307..29408)
                     /locus_tag="OQN12_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCS1653706.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29415..29588)
                     /locus_tag="OQN12_001312"
     CDS             complement(29415..29588)
                     /locus_tag="OQN12_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653707.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29706..30899
                     /locus_tag="OQN12_001313"
     CDS             29706..30899
                     /locus_tag="OQN12_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCS1653708.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="OQN12_001314"
     CDS             complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="OQN12_001314"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1653709.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32677..33867
                     /gene="hmpA"
                     /locus_tag="OQN12_001315"
     CDS             32677..33867
                     /gene="hmpA"
                     /locus_tag="OQN12_001315"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1653710.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="OQN12_001316"
     CDS             complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="OQN12_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1653711.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="OQN12_001317"
     CDS             complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="OQN12_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1653712.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="OQN12_001318"
     CDS             complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="OQN12_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1653713.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="OQN12_001319"
     CDS             complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="OQN12_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1653714.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38516..42403)
                     /gene="purL"
                     /locus_tag="OQN12_001320"
                     /gene_synonym="purI"
     CDS             complement(38516..42403)
                     /gene="purL"
                     /locus_tag="OQN12_001320"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1653715.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42662..44218
                     /gene="mltF"
                     /locus_tag="OQN12_001321"
     CDS             42662..44218
                     /gene="mltF"
                     /locus_tag="OQN12_001321"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1653716.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="OQN12_001322"
     CDS             complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="OQN12_001322"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1653717.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="OQN12_001323"
     CDS             complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="OQN12_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1653718.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45620..46468
                     /locus_tag="OQN12_001324"
     CDS             45620..46468
                     /locus_tag="OQN12_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1653719.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46807..47334)
                     /locus_tag="OQN12_001325"
                     /pseudo
     CDS             complement(46807..47334)
                     /locus_tag="OQN12_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11287 a        12962 c        11899 g        11186 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta cacatgagca acgcaggtgc ttcacagcaa
      601 aacaatactc accagtaact ctctttttgt caagcaaaag agagtaatta ttgtttattt
      661 agcgtattat cgacaccggc cctttccgcc gtgttcggta ataaaataac ctggcttatt
      721 agtccgaatt cagacaaata taaacaaatc ctgctcaaaa ttaaaaattc taaccggtaa
      781 aagatattac ttaaacatgt aaatttactt tcctttaaaa aacaaaaaac cgccaaaatc
      841 aggcggttat ttgttgctgg tccggttcgc ggcctttcca gcaggttgta ttaccgtagt
      901 aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa
      961 agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca
     1021 agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg
     1081 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
     1141 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
     1201 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
     1261 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt
     1321 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
     1381 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
     1441 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
     1501 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
     1561 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
     1621 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
     1681 ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt
     1741 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
     1801 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
     1861 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
     1921 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
     1981 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
     2041 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
     2101 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
     2161 tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt
     2221 acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact
     2281 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
     2341 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
     2401 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg
     2461 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagt aaacgaccgg
     2521 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
     2581 ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca
     2641 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
     2701 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
     2761 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cataatggca aatgccagcg
     2821 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
     2881 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
     2941 ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
     3001 ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
     3061 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
     3121 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
     3181 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
     3241 ccactttacg cgtgcctttc gggcctttat aaccttgcca gaatgcccag cagcgatccg
     3301 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcggg
     3361 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
     3421 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
     3481 taatcttcat cgcttctgtc attttagtta tccttattcg tttaacagca aaacgggcta
     3541 cccccaggca gcccgttcat tcactcggct tcgtccagcc agactaacaa aatcgcttcg
     3601 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
     3661 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
     3721 gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct
     3781 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
     3841 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
     3901 cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg cagtggcagg
     3961 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc aataccgtta cgcagcgctg
     4021 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagaggtcct
     4081 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
     4141 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
     4201 tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc actcaggtga
     4261 gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc atcagcagcc
     4321 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcata
     4381 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
     4441 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
     4501 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
     4561 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
     4621 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
     4681 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
     4741 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc
     4801 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
     4861 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
     4921 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
     4981 tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc
     5041 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
     5101 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggt ggcatccagc
     5161 agttcacgct gaacgcggtt atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa
     5221 tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc
     5281 acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg
     5341 tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagca
     5401 gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg
     5461 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
     5521 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
     5581 accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
     5641 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
     5701 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
     5761 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
     5821 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
     5881 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
     5941 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
     6001 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca ccgtatccgc
     6061 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
     6121 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
     6181 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
     6241 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
     6301 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
     6361 ttgttccgcc aggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
     6421 aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
     6481 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
     6541 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
     6601 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
     6661 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
     6721 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
     6781 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
     6841 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
     6901 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
     6961 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
     7021 ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga gagacttccc
     7081 tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc cgtaagtttt
     7141 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcattacgtc
     7201 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tatcaaagga
     7261 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
     7321 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
     7381 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
     7441 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
     7501 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
     7561 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
     7621 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
     7681 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
     7741 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc
     7801 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
     7861 gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga cgcacataca gcgcaccgat
     7921 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
     7981 caggtcgatt ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
     8041 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
     8101 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc gcttcgagtt ctttcaggtc
     8161 gataataccg ttacgctgcg gtgccaggta ggtgacttcg aaaccttcgc gctccagctg
     8221 acggcaagta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
     8281 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
     8341 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
     8401 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
     8461 ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt ccaccggcgt
     8521 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
     8581 tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga gtatttagca
     8641 ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg
     8701 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
     8761 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
     8821 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
     8881 acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
     8941 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
     9001 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
     9061 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
     9121 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
     9181 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
     9241 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
     9301 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
     9361 gacctacgat tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
     9421 cactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
     9481 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
     9541 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
     9601 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
     9661 tgctcgccat tttcctgagt tgccagccag gccatacgca cttcataggc aataacctga
     9721 accgccattg ccaggttcag cgaactgtat tccgggtttg ccgcaatcgc gacatgataa
     9781 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
     9841 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgcgcgg gtcgagcatc
     9901 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
     9961 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgcagcc
    10021 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    10081 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    10141 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    10201 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    10261 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    10321 cgcaaggcgg gtaatttagt tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    10381 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    10441 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    10501 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    10561 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    10621 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcaaggc
    10681 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    10741 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    10801 ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    10861 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    10921 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    10981 ggtcataact atatgctgac cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc
    11041 atactggcga acatgcgtga cgagttaagc gacgctctga agcattaatg actcaggcgg
    11101 gtgatatcac tcacccgccc tcgcctttca ggcgctattc cgaaatactt cctcaccgct
    11161 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    11221 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    11281 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    11341 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    11401 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    11461 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    11521 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    11581 ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg acgatacgca aagcgccatc
    11641 aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc tggtgctgtt cgggcagagt
    11701 attggcgggg cgaatattct tgctgttatt ggtcagggtg atcgtgaagg catacgtgcg
    11761 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    11821 agtggctact tacttgatga gagttacagc ggcgaaaatt acatcgccag cgtcagcccg
    11881 atcccgcttt tactcattca cggtaaagcc gatcacgtta tcccatggca gcacagcgaa
    11941 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    12001 attgatgctt tttccgatcg tcacggcaat gtttatcgcg aacagatggt ggactttatc
    12061 cttagcgcgt tgaatccgca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    12121 accaccttaa gacatgctaa tccactggtc agaacagttt aaaatgagaa aaattctgtg
    12181 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    12241 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    12301 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    12361 gttgaccgcc accatggcaa ccttcagcga gcttaatggt gtggatgatg atattgccag
    12421 tcttgatatc agcgaaacag gacgggagat cctgcgctat catcaactca cactgacgac
    12481 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    12541 tcactggcta cgacgtggtt tccgtctgtg tccgtcattt attaccagcc aattcacccc
    12601 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    12661 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aatcgcgcga
    12721 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg cagcaccacg ctggaattac
    12781 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    12841 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag catgtaccgc ctgatgagcc
    12901 actgtttatg gcattacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    12961 tcagcgggac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    13021 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata tccacctcgc
    13081 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    13141 cgccagactg tatccacgcc tggtacgcac cacccgcgcg gcgctggccg gatttgaaag
    13201 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    13261 ctggctaatg caggaaaacg atctgcatga aaaacagatt attctactga ccgggaatga
    13321 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    13381 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    13441 gattattgcg ccttatacca tgccgttacc gctcttttca ccaccgctga tctatacgga
    13501 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    13561 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    13621 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gcgatactgc
    13681 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta acctggcgag
    13741 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    13801 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    13861 cgcaaatcgc cgagatcaac agcatatcgc gagcactaca acggcggaaa agtttattac
    13921 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    13981 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    14041 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggcgaacc agcgccaacc
    14101 acgcagacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    14161 ggatgagaaa gccgagcagc atggatgcca cgcccaacgt caacagcacg agaatcaccc
    14221 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    14281 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    14341 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    14401 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt ggcagcagtg
    14461 ccagcacgcg caaggcggaa atcaggcggg aaggatcgct gacgcggggc gcgatgagca
    14521 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    14581 ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa aaaaatgccg taactaaaaa
    14641 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    14701 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    14761 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    14821 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gcttgccaaa cgttcctgca
    14881 caatggcgat gaaatcgcgt aatgccagct tcatctcccc tttcttccac gccatcagta
    14941 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat
    15001 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    15061 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    15121 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    15181 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    15241 ccaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    15301 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    15361 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    15421 ttggcaataa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    15481 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    15541 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    15601 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    15661 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    15721 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    15781 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    15841 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    15901 ttaccaactg attgacaccc aaaacggtcg ggtcactccg cgtatttata ccgacccgga
    15961 catttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    16021 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcagttgt
    16081 cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    16141 catgcgtgtg agttatgcag attgtggcaa cactcgcgcc tttacctgcc cgtatcacgg
    16201 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    16261 acaagggttg tgtaaatccc actgggggct aaacgaagtt ccttgtgtgg agagttataa
    16321 aggactgatt tttggcaact gggataccag cgcaccgggc ctgcatgatt acctgggtga
    16381 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    16441 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    16501 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    16561 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    16621 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa
    16681 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    16741 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    16801 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    16861 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    16921 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    16981 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    17041 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    17101 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    17161 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    17221 ggaggtgatg aaatgagtgc gcaagtttca ctggagttac atcaccgcat tagccagttt
    17281 ctctttcacg aagccagtct gctggatgac tggaaatttc gtgactggtt ggcacagctc
    17341 gacgaagaga ttcgttacac catgcgcacc acggttaacg cgcaaacacg cgaccgtcgc
    17401 aaaggcgtcc agccgccgac gacctggatt ttcaatgaca ccaaagacca gctggagcgg
    17461 cgaatcgccc gtctggaaac gggcattgcc tgggcagaag agccgccgtc acgcactcgt
    17521 cacttaatca gcaactgcca ggtaagcgaa accgacatcc caaacgtatt tgccgtgagg
    17581 gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    17641 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    17701 gtcctggatc aagcggtgat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    17761 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggata gatacctcgc
    17821 ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc cattaacgat cgttgcagcc
    17881 atggtaatgc gtcgatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    17941 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    18001 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    18061 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    18121 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caagttgcca cgctggaact
    18181 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    18241 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    18301 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    18361 taatactccc gcagagacgc tcgaaaccgg cttccacgag ctgtttaacg tcaacgtact
    18421 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    18481 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccac
    18541 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    18601 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    18661 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    18721 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    18781 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    18841 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    18901 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    18961 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    19021 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    19081 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    19141 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    19201 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    19261 ctgctttacc ctacaccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    19321 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    19381 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    19441 accaccgccc gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct
    19501 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    19561 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    19621 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    19681 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    19741 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    19801 cgcaatgtta gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta
    19861 cagtgataac ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa
    19921 cccggaaact cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt
    19981 cacgctgaat cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac
    20041 gtttgatgcg aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata
    20101 attagcgaat atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa
    20161 atcacattat cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc
    20221 tgcccgcccc gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt
    20281 tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc
    20341 gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt
    20401 aatcgtgctt ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg
    20461 tacggcggtg attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat
    20521 gattaatttc tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg
    20581 accgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct
    20641 tatccggcat tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga
    20701 ttcccctggt gctagcgcaa tgagatcacc cccttccggg cgatgatgat catccggcgc
    20761 atggctcatc ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc
    20821 ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc
    20881 ttcctgcggc tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca
    20941 ctgacgcggc aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa
    21001 ctcacccgcc agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg
    21061 cgtttgtggt gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc
    21121 cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg
    21181 acttacccga tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc
    21241 gctgtgcgag acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc
    21301 ccactcaaca ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct
    21361 cacccggttt gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt
    21421 tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag ccttcgatta cgccgccctg
    21481 gtcagaaacg tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact
    21541 ccttaatccg ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag
    21601 gcatcacctg cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc
    21661 cagtcagtca aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg
    21721 ataatccgcc gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt
    21781 tcaccaatgt aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct
    21841 gcggcattac cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt
    21901 atctgcggca gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt
    21961 gccatcgcca gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct
    22021 ttcgccagca tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg
    22081 ctgccggaaa cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata
    22141 aacgcaccat cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc
    22201 agcagatatt cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca
    22261 gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga
    22321 tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc
    22381 tgcccgcacg gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc
    22441 gccgtggcac ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat
    22501 ttcattttga tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg
    22561 acggttgaat ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca
    22621 atacgattaa ttttcatccc tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg
    22681 ctttactgct tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc
    22741 cgcgtaccac ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta
    22801 gcgtaatcac cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg
    22861 ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg
    22921 cgttaccgga accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg
    22981 aaagggtaaa gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg
    23041 tggcattacc gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca
    23101 caaacagcgc aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc
    23161 cgcccagcca gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa
    23221 acagccccat tccgcgcaga gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt
    23281 taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca
    23341 gcagacaagc aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga
    23401 caaaagccac catcggccca acgctgacat caatttcacc ggagataata atcagcgtca
    23461 tcgcccaggc ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca
    23521 atgagataaa gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca
    23581 gcaaaccgat ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct
    23641 gcggcaatgg taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgta
    23701 cagacagaat ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga
    23761 acgtgccgtg ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac
    23821 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    23881 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    23941 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga
    24001 tgggtgtttc gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc
    24061 ggcggatggt ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca
    24121 ctgtattttc gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg
    24181 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt
    24241 tgataacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    24301 tgcgccctgc ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc
    24361 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca
    24421 caatttcctg aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca
    24481 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    24541 taacggtggc ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca
    24601 ccgccacgcc cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac
    24661 tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    24721 gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    24781 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    24841 caatcatgcc gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc
    24901 cttccaccag acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag
    24961 tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    25021 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    25081 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    25141 cggggtaacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    25201 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    25261 tgtttaccat cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg
    25321 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    25381 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    25441 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    25501 tttaccgcgc cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg
    25561 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta
    25621 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    25681 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    25741 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagtggatcg
    25801 ccgaccagat acgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    25861 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    25921 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    25981 ctttctttcg aaatatcccc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    26041 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg ggtatcaagg
    26101 tatattgcac caatggtcat ttctttatcc gcagccctgg caaatagtgc gctacctaat
    26161 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agtgcctcct
    26221 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    26281 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    26341 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    26401 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    26461 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
    26521 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    26581 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
    26641 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    26701 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
    26761 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    26821 ttctccggat aatacagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    26881 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    26941 tccccgcctt cccacgggta gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
    27001 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    27061 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    27121 tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    27181 tcaagataac gcgcggccag ctcataatcg tgtttcttat tccacgcatg gatcgctaac
    27241 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    27301 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    27361 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    27421 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    27481 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    27541 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg
    27601 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    27661 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    27721 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaagca aagcacttcc
    27781 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    27841 tcattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttact attgccgctc
    27901 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27961 ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt
    28021 ttcagcgcct gactggcata cgccaccgct tgcgggaaat ctgcgcggtt atattccagc
    28081 atcgccagcg ccaggttaca gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag
    28141 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    28201 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    28261 ggcaactcct gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    28321 agttcaatgg tcagtctgtc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    28381 ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc
    28441 agacggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    28501 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    28561 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    28621 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28681 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28741 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
    28801 acaaaatcgt actgcgattt ttcggccata tatgaggttg gaacaggcac atttttatag
    28861 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28921 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
    28981 ctctgatgcc cttcaccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    29041 gtggcgttgc cgttatagac gcggctggcg atttccagtg ccgcccggtc agggcgcagg
    29101 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    29161 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    29221 tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc acggtcccag catccccacc
    29281 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    29341 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    29401 agccacaccg cctgctagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    29461 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    29521 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    29581 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    29641 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29701 tgaagatgag agcctgcatt aacaatcaac agattctcca ccataacaaa tgcgtgattc
    29761 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29821 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29881 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29941 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    30001 atgcttgttt aagtccgaaa ggcgaatttg agtatttaca gattgatgta ccgacgccgc
    30061 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    30121 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    30181 aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt gaagtatctg ctggaagaga
    30241 agctgggcat tcgggtgctg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    30301 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    30361 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    30421 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggcagcctgg
    30481 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    30541 cggttaatac tcagcttgag cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
    30601 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    30661 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    30721 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    30781 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
    30841 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    30901 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    30961 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    31021 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    31081 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    31141 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    31201 cagccagttc tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta
    31261 cacgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    31321 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa
    31381 ccagatcaac caggaacagg tggttatcag tgccgccgga aaccactttg tagccgcgct
    31441 cgaggaacac ttctaccatc gctttagcgt ttttcgcgac ctgctgctgg taagttttga
    31501 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    31561 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    31621 cacctttcgc caagatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    31681 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    31741 cgtgcgccat atcaacgaac agataagcac cgatgctgtc agcgatttca cgcattttcg
    31801 cccagtcaac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    31861 ctttggcttg tttttccaga tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag
    31921 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    31981 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    32041 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    32101 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    32161 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    32221 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    32281 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    32341 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    32401 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    32461 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    32521 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    32581 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    32641 agatacatca attaagatgc aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct
    32701 acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc
    32761 tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag
    32821 cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt
    32881 gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc
    32941 cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa
    33001 atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct
    33061 aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt
    33121 tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc
    33181 agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat
    33241 ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg
    33301 actcgcaaac cggatggcaa aggctatcgt atcgcggtga aacgcgaaga gggtgggcag
    33361 gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg
    33421 gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctaccggt
    33481 gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca
    33541 caagtgaact ggttccatgc ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt
    33601 aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc
    33661 gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg
    33721 gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg
    33781 cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa
    33841 tgctttggcc cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc
    33901 cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga
    33961 gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata
    34021 atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc
    34081 ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga
    34141 ccaaagcctt tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg
    34201 cggacatcgt ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt
    34261 gaaaaggttg cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg
    34321 aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc
    34381 cccgccattc tagccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa
    34441 tagttgagtt caaaatgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc
    34501 tccagcgcct gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac
    34561 tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt catcaggcgt
    34621 ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc
    34681 aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc
    34741 aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct
    34801 tttgacagat cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta
    34861 ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc
    34921 gcaggcatat cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa
    34981 ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac
    35041 tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc
    35101 gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta
    35161 atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc
    35221 agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc
    35281 tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa
    35341 ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga
    35401 ataattactg gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc
    35461 atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt
    35521 aatccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg
    35581 ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg
    35641 ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc
    35701 cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt
    35761 ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa
    35821 tgtatccagc tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc
    35881 cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc
    35941 cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt
    36001 aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc
    36061 atattggcga gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc
    36121 ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct
    36181 acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt
    36241 atcaatggct ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc
    36301 tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact
    36361 taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga
    36421 aaccggggcg tacatatcgt acgtgaagat ttcgagcact acccggggcc gaactgacaa
    36481 ataaaatagc ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat
    36541 tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata
    36601 cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt
    36661 cgctggtggc ttccctgaaa aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg
    36721 ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg
    36781 caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg
    36841 ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc
    36901 atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct
    36961 aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt
    37021 tgttcgatca gtttttgcaa attgcagctg ctgctatcaa gaatgctcac cacctctttt
    37081 tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata
    37141 ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat
    37201 tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg
    37261 ctcggtccac agaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc
    37321 atgcgctcga tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc
    37381 agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt
    37441 tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg
    37501 gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg
    37561 ttacactgaa gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg
    37621 tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga
    37681 taaaccttcg caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc
    37741 tccagcgccg cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta
    37801 accagcgccg cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc
    37861 aacaggggta gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga
    37921 aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc
    37981 gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg
    38041 gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga
    38101 cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac
    38161 ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat
    38221 agtttggtaa cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg
    38281 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta
    38341 caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    38401 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    38461 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    38521 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    38581 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    38641 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    38701 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    38761 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    38821 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    38881 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    38941 gccacaactc actccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    39001 atacccccag ggacagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    39061 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    39121 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    39181 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    39241 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    39301 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    39361 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    39421 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    39481 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    39541 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    39601 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    39661 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    39721 ccagcgttac cagcaggcct ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    39781 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    39841 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    39901 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    39961 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    40021 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    40081 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    40141 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    40201 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    40261 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    40321 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    40381 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    40441 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    40501 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    40561 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    40621 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    40681 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    40741 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    40801 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    40861 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    40921 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    40981 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    41041 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    41101 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    41161 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    41221 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    41281 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc agtaacaatg cgctcaggct
    41341 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    41401 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    41461 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    41521 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    41581 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    41641 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    41701 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    41761 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    41821 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt
    41881 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    41941 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    42001 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    42061 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    42121 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    42181 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    42241 gttgtgcgtg ctcatcatcg tttaacgacg cattgaggtc agcaaaatgg acatactcgg
    42301 cgtaaatatt gtgaaccggg agcctagcag cctgaaaacg tgccagcagt ttgttgattc
    42361 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    42421 agctttcggt gtacccaagg ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg
    42481 acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat
    42541 ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa
    42601 acctttcact attattacgt tttttcaagc tgggacgcgc acgacacaga gaattaacta
    42661 attgaaaaaa ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt
    42721 cgctctctgg ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca
    42781 agcgcgggga gagttgcgtg tgagcaccat tcatactccc ctgacttata acgaaatcaa
    42841 cgggaaacct tttggcctag attacgaact ggcgaaacag tttgccgatt acctcggcgt
    42901 aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg tttgacgacc ttgataatgg
    42961 taacgccgac ctgctggcgg caggactggt ctataacagt gagcgggtaa aaaattatca
    43021 gcctggccct acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg
    43081 cccacgtacg ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt
    43141 ggttaacgat ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga
    43201 cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac
    43261 cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc
    43321 gctcgatatc accgatgaac aaccggtgac ttggtttagc ccgttagatg gcgataatac
    43381 cctttccgcc gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg
    43441 cattgaagag aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt
    43501 tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga
    43561 agaaattgac tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca
    43621 ggccacttca ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag
    43681 cctcggcatt acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt
    43741 gcaggatatg atgagtaaag tgccggaaag tgtgccggag aacgaacgga tctggtttgc
    43801 cctcgctgcg tacaatatgg gctatgcgca tatgctggat gcccgcgccc tgacggcaaa
    43861 aaccaaaggg aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca
    43921 gaaaccctat tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta
    43981 tgtcgaaaat attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa
    44041 gcaggctaca gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga
    44101 gttgggcaaa gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt
    44161 gacccattct ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc
    44221 cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc
    44281 actgagcaac gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt
    44341 catacccgga tgatgcagca catccattaa agatcccaca gcgccagttt tcgcgtcacg
    44401 cgcaccaaag accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca
    44461 tggttcaagc gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc
    44521 accctgccgc agggccatga tttctgcatg tgcggtggga tcatggcgac caatcgggcg
    44581 gttccagcct tcgccgatta cccgattgtt atgcactaat accgcgccga ccggcacttc
    44641 ccgctcatcc caggcacgtt tcgccagcgt cagcgcgtga cgcatccaat attcgtggct
    44701 aaattcgact tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg
    44761 acgctatgct ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccaacg
    44821 atgctgacag aaataaagca gcggattgtc ctgattactg tcgctatagc cactgtacag
    44881 ccgcagcggc gtgccgattt tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg
    44941 catcgtcaat acccaaccac catagccacg ctgaatttgg ctggcgataa gattaacccg
    45001 cggcagccag ggcgtatcga aataaaccgc ttcaaccagc ggctgcggag agccggtaat
    45061 caaccagata tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg
    45121 aaaggcggta acattgtcgc gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg
    45181 tgcttcgctg tgaccaaaag tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc
    45241 acgacctttt atcaataacg caatggctat aatcggtaac aacggcagga caagtaacgc
    45301 attcagcggt tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg
    45361 caatgttcca tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc
    45421 ctctggactg aaacatcctg attaattttc gctactacat agcctaacag atagatcatc
    45481 acttttccgg caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt
    45541 agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta
    45601 tttcatcgcg aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaagggct
    45661 tgcccagagt gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca
    45721 tttaagctct cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt
    45781 cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct
    45841 ggcaagccag ccggaatcgc cctccgtgcc cattcataac caaatccgcg gtgatgatcc
    45901 gttacggctg gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct
    45961 aaacgttaat agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg
    46021 gataattctg accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct
    46081 gatgaagatt ggcttcaatg ctgccgcact gcgcgatatg catgcgctac tcgcaacagt
    46141 acaggcgtcg tcccctgacg atctgttatt agccatttcc tacaccggtg tacgacgcga
    46201 gttaaacctg gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg
    46261 ctttactccg aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga
    46321 acaagcgaca aacagtgctt caatctctgc ttgtcacgct cagggaatgt taacggattt
    46381 gctgttcatt gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag
    46441 tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt
    46501 tgtgttgttt tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg
    46561 atatgtgtga aatcaaataa aaacacattt attttcatag tgatacagca atgtgttatt
    46621 gaatgtgttg ttggatttat ggtgcaaaaa aattttgcat aagttcagca acacaaagaa
    46681 caccactttc tttaacgtcc tccgcgccct aaaggacggg gctttacggc gcaaaaggcc
    46741 tgtgacatac aacattaact tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct
    46801 gcggttttat ataaccctgg ctggaaacct cttatacaaa gtcgattcgc caacatcata
    46861 gatgatcgcc accttctggc gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca
    46921 ttattctggt gaaagtttgg gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc
    46981 cagtcctgct tttgttcgtt caacaatcag ttcacgttcc atttcagcca gggcacccat
    47041 cacatggaag aaaaagcgtc ccattggtgt gctggtatca attgaatcag tcagactacg
    47101 aaagttgatg cctcgttcgc gcaactcctc caccagcacg acaagatgcc gcatactgcg
    47161 ccccagccga tccagcttcc agacaaccag tgtgtcgcct gccgataatg tcttgagcag
    47221 ttttttcagc cccggtcttt cggactttgt accacttatc ttgtcttcaa aaatcagctc
    47281 gcatcctgca cagttcaacg cattacgttg tagatcggta ttctggtcat ttgt
//
LOCUS       DAJVRP010000024        46603 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVRP010000024 DAJVRP010000000
VERSION     DAJVRP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.024"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(19..606)
                     /gene="mog"
                     /locus_tag="OQN12_001326"
     CDS             complement(19..606)
                     /gene="mog"
                     /locus_tag="OQN12_001326"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1653720.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(721..1674)
                     /gene="tal"
                     /locus_tag="OQN12_001327"
     CDS             complement(721..1674)
                     /gene="tal"
                     /locus_tag="OQN12_001327"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1653721.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1953..3382
                     /locus_tag="OQN12_001328"
                     /pseudo
     CDS             1953..3382
                     /locus_tag="OQN12_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3452..4228
                     /gene="yaaA"
                     /locus_tag="OQN12_001329"
     CDS             3452..4228
                     /gene="yaaA"
                     /locus_tag="OQN12_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1653722.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4381..4677)
                     /locus_tag="OQN12_001330"
     CDS             complement(4381..4677)
                     /locus_tag="OQN12_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1653723.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4891..6177)
                     /gene="thrC"
                     /locus_tag="OQN12_001331"
     CDS             complement(4891..6177)
                     /gene="thrC"
                     /locus_tag="OQN12_001331"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1653724.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6178..7110)
                     /gene="thrB"
                     /locus_tag="OQN12_001332"
     CDS             complement(6178..7110)
                     /gene="thrB"
                     /locus_tag="OQN12_001332"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1653725.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7112..9574)
                     /gene="thrA"
                     /locus_tag="OQN12_001333"
     CDS             complement(7112..9574)
                     /gene="thrA"
                     /locus_tag="OQN12_001333"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1653726.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9600..9719)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9655..9720)
                     /gene="thrL"
                     /locus_tag="OQN12_001334"
     CDS             complement(9655..9720)
                     /gene="thrL"
                     /locus_tag="OQN12_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1653727.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9934..10620)
                     /locus_tag="OQN12_001335"
     CDS             complement(9934..10620)
                     /locus_tag="OQN12_001335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1653728.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11020..11160)
                     /locus_tag="OQN12_001336"
     CDS             complement(11020..11160)
                     /locus_tag="OQN12_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653729.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11256..11972
                     /gene="arcA"
                     /locus_tag="OQN12_001337"
     CDS             11256..11972
                     /gene="arcA"
                     /locus_tag="OQN12_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1653730.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12031..13383)
                     /gene="creD"
                     /locus_tag="OQN12_001338"
     CDS             complement(12031..13383)
                     /gene="creD"
                     /locus_tag="OQN12_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1653731.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13441..14865)
                     /gene="creC"
                     /locus_tag="OQN12_001339"
     CDS             complement(13441..14865)
                     /gene="creC"
                     /locus_tag="OQN12_001339"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1653732.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14865..15554)
                     /gene="creB"
                     /locus_tag="OQN12_001340"
     CDS             complement(14865..15554)
                     /gene="creB"
                     /locus_tag="OQN12_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1653733.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15567..16040)
                     /gene="creA"
                     /locus_tag="OQN12_001341"
     CDS             complement(15567..16040)
                     /gene="creA"
                     /locus_tag="OQN12_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1653734.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16251..17120
                     /gene="robA"
                     /locus_tag="OQN12_001342"
     CDS             16251..17120
                     /gene="robA"
                     /locus_tag="OQN12_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1653735.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17117..17764)
                     /gene="gpmB"
                     /locus_tag="OQN12_001343"
     CDS             complement(17117..17764)
                     /gene="gpmB"
                     /locus_tag="OQN12_001343"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1653736.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17816..18336
                     /gene="yjjX"
                     /locus_tag="OQN12_001344"
                     /pseudo
     CDS             17816..18336
                     /gene="yjjX"
                     /locus_tag="OQN12_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18421..18747)
                     /gene="trpR"
                     /locus_tag="OQN12_001345"
     CDS             complement(18421..18747)
                     /gene="trpR"
                     /locus_tag="OQN12_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1653737.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18837..20774)
                     /gene="sltY"
                     /locus_tag="OQN12_001346"
     CDS             complement(18837..20774)
                     /gene="sltY"
                     /locus_tag="OQN12_001346"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1653738.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20985..22652
                     /gene="ettA"
                     /locus_tag="OQN12_001347"
     CDS             20985..22652
                     /gene="ettA"
                     /locus_tag="OQN12_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1653739.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22759..22926)
                     /locus_tag="OQN12_001348"
     CDS             complement(22759..22926)
                     /locus_tag="OQN12_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653740.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(22960..24192)
                     /gene="nadR"
                     /locus_tag="OQN12_001349"
     CDS             complement(22960..24192)
                     /gene="nadR"
                     /locus_tag="OQN12_001349"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1653741.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24213..25595)
                     /gene="radA"
                     /locus_tag="OQN12_001350"
     CDS             complement(24213..25595)
                     /gene="radA"
                     /locus_tag="OQN12_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1653742.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25644..26612)
                     /gene="serB"
                     /locus_tag="OQN12_001351"
     CDS             complement(25644..26612)
                     /gene="serB"
                     /locus_tag="OQN12_001351"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1653743.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26718..27362
                     /locus_tag="OQN12_001352"
     CDS             26718..27362
                     /locus_tag="OQN12_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1653744.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27390..28405
                     /gene="lplA"
                     /locus_tag="OQN12_001353"
                     /pseudo
     CDS             27390..28405
                     /gene="lplA"
                     /locus_tag="OQN12_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28437..28700
                     /locus_tag="OQN12_001354"
     CDS             28437..28700
                     /locus_tag="OQN12_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1653745.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28861..29580)
                     /gene="deoD"
                     /locus_tag="OQN12_001355"
     CDS             complement(28861..29580)
                     /gene="deoD"
                     /locus_tag="OQN12_001355"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1653746.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29637..30860)
                     /gene="deoB"
                     /locus_tag="OQN12_001356"
     CDS             complement(29637..30860)
                     /gene="deoB"
                     /locus_tag="OQN12_001356"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1653747.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30912..32234)
                     /gene="deoA"
                     /locus_tag="OQN12_001357"
     CDS             complement(30912..32234)
                     /gene="deoA"
                     /locus_tag="OQN12_001357"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1653748.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32361..33140)
                     /gene="deoC"
                     /locus_tag="OQN12_001358"
     CDS             complement(32361..33140)
                     /gene="deoC"
                     /locus_tag="OQN12_001358"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1653749.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKLAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33398..34939
                     /locus_tag="OQN12_001359"
     CDS             33398..34939
                     /locus_tag="OQN12_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1653750.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34911..35774
                     /locus_tag="OQN12_001360"
     CDS             34911..35774
                     /locus_tag="OQN12_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1653751.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35988..36770)
                     /locus_tag="OQN12_001361"
     CDS             complement(35988..36770)
                     /locus_tag="OQN12_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1653752.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36767..37840)
                     /locus_tag="OQN12_001362"
     CDS             complement(36767..37840)
                     /locus_tag="OQN12_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1653753.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37962..38123)
                     /locus_tag="OQN12_001363"
     CDS             complement(37962..38123)
                     /locus_tag="OQN12_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1653754.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38240..38845)
                     /gene="osmY"
                     /locus_tag="OQN12_001364"
     CDS             complement(38240..38845)
                     /gene="osmY"
                     /locus_tag="OQN12_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCS1653755.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39238..40827)
                     /gene="prfC"
                     /locus_tag="OQN12_001365"
     CDS             complement(39238..40827)
                     /gene="prfC"
                     /locus_tag="OQN12_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1653756.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40918..41595)
                     /gene="yjjG"
                     /locus_tag="OQN12_001366"
     CDS             complement(40918..41595)
                     /gene="yjjG"
                     /locus_tag="OQN12_001366"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1653757.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41610..42056)
                     /gene="rimI"
                     /locus_tag="OQN12_001367"
     CDS             complement(41610..42056)
                     /gene="rimI"
                     /locus_tag="OQN12_001367"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1653758.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42025..42438)
                     /gene="holD"
                     /locus_tag="OQN12_001368"
     CDS             complement(42025..42438)
                     /gene="holD"
                     /locus_tag="OQN12_001368"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1653759.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42541..43572
                     /gene="rsmC"
                     /locus_tag="OQN12_001369"
     CDS             42541..43572
                     /gene="rsmC"
                     /locus_tag="OQN12_001369"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1653760.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            43849..43935
                     /locus_tag="OQN12_001370"
     tRNA            43849..43935
                     /locus_tag="OQN12_001370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43883..43885,aa:Leu,seq:cag)
     gene            43964..44050
                     /locus_tag="OQN12_001371"
     tRNA            43964..44050
                     /locus_tag="OQN12_001371"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43998..44000,aa:Leu,seq:cag)
     gene            44085..44171
                     /locus_tag="OQN12_001372"
     tRNA            44085..44171
                     /locus_tag="OQN12_001372"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44119..44121,aa:Leu,seq:cag)
     gene            complement(44210..44446)
                     /locus_tag="OQN12_001373"
     CDS             complement(44210..44446)
                     /locus_tag="OQN12_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1653761.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            44587..45375
                     /gene="fhuF"
                     /locus_tag="OQN12_001374"
     CDS             44587..45375
                     /gene="fhuF"
                     /locus_tag="OQN12_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1653762.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(45413..46090)
                     /gene="bglJ"
                     /locus_tag="OQN12_001375"
     CDS             complement(45413..46090)
                     /gene="bglJ"
                     /locus_tag="OQN12_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCS1653763.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(46048..>46603)
                     /locus_tag="OQN12_001376"
     CDS             complement(46048..>46603)
                     /locus_tag="OQN12_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1653764.1"
                     /translation="DLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELL
                     MCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSER
                     KVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGAREL
                     NLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        11041 a        12698 c        11727 g        11137 t
ORIGIN      
        1 ccgctcgggg ggattttttt attcgctaac gtcgcgtctt gcactcttcg gtctgaatgc
       61 agcaaccact tctggtgccg tttcaacgta tggcccttcc agcaactgaa tgcagtacgg
      121 tacgctggca aaaataccat gcaccacaac gttaccctca gcgtccttca caccttccag
      181 cgtctcttta atagacttcg gctgaccggg taagttaagg atcagcgcct gtttgcgaat
      241 cacgcccacc tgacgcgaaa ggatcgcagt tggtacaaaa tgcaggctga tctggcgcat
      301 ctgttcacca aagccaggca tctcgcggtc cgctactgcc agcgtcgcat cgggcgttac
      361 gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc agatggcaac tcatttcatc
      421 caccagctca cacaacgttt gctcgatgat cgcctgctca tcggggatta agcgggtttc
      481 cagttcaaac ggcgtggtta gcgccgatgt cagccattct tccagcgctg gaatgccttt
      541 atcctgataa acgccgctgg atgcgcgatc agagatggaa actaagccaa tacgtaaagt
      601 attcatcaag atttttccgt ttaaacaatc tcgttaaaca gaatgataca ctgcgaaggg
      661 agtgacagac aggcttcaga agaggtagcg tgaccgactt cccggtcacg ctaagaatga
      721 ttacagcaga tcgccgatca ttttttccag tttttcctgg tcaatagcaa acttacggat
      781 accttccgcc agtttatcta ctgccattgg atcctggttg tgctgccaca ggaactcgga
      841 ctcagtgata cgcgccggac gcgctttcac ttcgccggtg taagacagtt tacgttcgat
      901 agccccttcg ctctccgcca gctctttcag cagtgccggt gcgatggtca gacggtcaca
      961 gcctgccagt tccagaattt cgccgatgtt acggaagctt gcgcccataa ccacggtttc
     1021 ataaccgtgc tctttgtagt actggtagat ttcagataca gaaaccacgc ccggatcttc
     1081 tgccggagcg tactctttct tatcggtatt cgctttgtac cagtcaagaa tacggccaac
     1141 aaacggcgag atcaggaaca cgcccgcctc cgcacaagca cgagcctgag cgaaggagaa
     1201 cagcagggtc aggttacagt tgataccttc tttttccagc tgttctgcag cacggatacc
     1261 ctgccaggta gaagccagtt tgatcagaat acgatcgttg ctaatacctg catcgttgta
     1321 gagtttgatc aggcgttttg ctttcgcaat tgacgcttcg gtgtcatagg aaagacgcgc
     1381 atcaacttca gttgagatac ggcccggaac cagtttcagg atttccagac caatatttac
     1441 tgccagtttg tcggtcgcgt ccacgatctg ctgcgcgcga tcgttgctct gctgtttcgc
     1501 ccaggcgaca gcatcatcaa tcaacttacg gtattccgga atctgcgctg cgttaagaat
     1561 gagagaaggg ttggttgtgg catcctgcgg ttgatacagc ttcattgccg cgatgtcccc
     1621 agtgtcggcc actacggtgg tgtactgacg aagggaggtc aatttgtccg tcatgatagt
     1681 atttctcttt aaacagcttg ttagggggat gtaaccggtc tgccctgatg atatcacgac
     1741 gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag gcgagactgt ttcggatttc
     1801 tgacactgcg ttgatatcgc ccaccatttt tatacaaaac ctcatgtatg ctacgcagaa
     1861 gttatcaagt acctcgtagc gtatatactt cttaaacaat tggtaacgtt tacacaggaa
     1921 agtcatcgcg accggcaata agagggatat gcatgccaga ttttttctcc tttattaaca
     1981 gcgtcctttg gggatcggta atgatttacc tgctcttcgg cgcaggttgt tggttcactt
     2041 ttcgcaccgg atttgtgcag tttcgctaca tccgccagtt tggcaaaagt cttaaaaata
     2101 gcattcatcc acagccaggc ggtttaacct catttcagtc attgtgtacc agtcttgcgg
     2161 cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc aattaccgcc ggtggaccag
     2221 gtgccgtctt ttggatgtgg gttgctgcgt ttatcggcat ggcgacctcg tttgccgaat
     2281 gttcccttgc acaactttat aaagaacgtg acgtcaatgg gcagtttcgt ggcggaccgg
     2341 catggtatat ggcgcgcggg ctggggatgc gctggatggg cgttctgttc gccgtctttt
     2401 tgctcatcgc ctacggcata attttcagcg gagttcaggc gaacgccgtt gcccgagccc
     2461 tgagtttttc ttttgatttt cctccgctgg tgacgggcat tattctcgct gtctttgctc
     2521 tgctggcaat cactcgcggt cttcatggcg tcgcccggct catgcagggg tttgtcccgt
     2581 tgatggcgat aatctgggta ctgaccagcc tggtgatttg cgtaataaat atcgggcaac
     2641 ttccccacgt catttggtct atttttgaga gtgcttttgg ctggcaggaa gcggcaggcg
     2701 gcgcggcggg atatacctta agccaggcga ttactaacgg ttttcagcgc agtatgtttt
     2761 ccaatgaggt gggaatggga tcgacgccaa acgcggcagc ggcagctgca tcctggcctc
     2821 cgcatccggc agcacaaggg attgtccaga tgattggcat ttttatcgac accctggtca
     2881 tctgtacggc aagcgccatg ctgatattac tggcgggtaa cggcacaacc tacatgccgc
     2941 tggaaggtat tcagcttatc cagaaggcga tgcgggtgct aatgggttcc tagggtgctg
     3001 aatttgttac cctcgtggtt attctgtttg ccttcagctc catcgttgcc aactacattt
     3061 atgccgaaaa caatctcttc tttttacgcc tgaacaaccc taaagcgatc tggtgtttgc
     3121 ggatctgcac cttcgcaacg atcatcggcg gcaccttgct aagccttccg ctgatgtggc
     3181 aactggcaga tatataatgg cctgcatggc tattaccaat ttgaccgcca ttttactgct
     3241 ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg
     3301 cccggtgttt gatccgttgc gttatccgga tatcggtcgc caactttctc cggacgcgtg
     3361 ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa
     3421 agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac
     3481 gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa
     3541 ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta gcacgctgat
     3601 gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga
     3661 tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg tctacaccgg
     3721 cttgcaggcc gagaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat
     3781 gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga
     3841 aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat
     3901 catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg tgattaatct
     3961 ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa
     4021 gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct acgctaagaa
     4081 agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac cagagcaact
     4141 gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca atggcgaact
     4201 ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg
     4261 ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac gtaggcctga
     4321 taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat
     4381 ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg
     4441 gtggcgcggc ggtgacggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg
     4501 ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc
     4561 acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc
     4621 catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg
     4681 ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga
     4741 cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat aatttctcct
     4801 ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa
     4861 acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa
     4921 cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag
     4981 ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc
     5041 cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc
     5101 acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat
     5161 tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat
     5221 tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc
     5281 gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg
     5341 cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgaatgactt
     5401 cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg
     5461 gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag
     5521 caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt tcagttcttc
     5581 atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac
     5641 agtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc
     5701 tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat gagccactgc
     5761 agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat ccgcaatatg
     5821 ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg
     5881 gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa
     5941 cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa taaacgccga
     6001 gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag
     6061 gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt
     6121 tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta
     6181 gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg
     6241 attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc
     6301 acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcactaccg cgccgatttc
     6361 cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc
     6421 gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat
     6481 gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa
     6541 aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca cccacagcca
     6601 ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat
     6661 accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc
     6721 cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt
     6781 catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg
     6841 catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg
     6901 ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg
     6961 tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa
     7021 tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc tcatattggc
     7081 actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac
     7141 gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca tatccgcgca
     7201 gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt
     7261 tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg cagacaccat
     7321 cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc
     7381 gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct
     7441 cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac
     7501 gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca gaaagatcat
     7561 ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc tcggagaaac
     7621 tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga
     7681 acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata accggtaatc
     7741 cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcataacgca
     7801 actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgta acaacgtgga
     7861 aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa
     7921 caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa
     7981 acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg ccatgcacac
     8041 tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata tgtttattct
     8101 tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc
     8161 caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca
     8221 cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct
     8281 gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga
     8341 tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca
     8401 ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc
     8461 gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg
     8521 attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg
     8581 ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc atgttattca
     8641 gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca atgagcgtac
     8701 ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg
     8761 gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct
     8821 ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga
     8881 ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg
     8941 ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt tcattaccgg
     9001 cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc
     9061 gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt
     9121 cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg
     9181 cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc
     9241 ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag
     9301 ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg
     9361 caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt
     9421 caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct
     9481 gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg
     9541 ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg
     9601 aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc
     9661 cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat
     9721 ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt
     9781 atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag
     9841 ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa
     9901 tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc
     9961 atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgctg
    10021 taaccagtcg accagtttta tgtcatctgc cactgccaga gccgtcagca atgccatgac
    10081 tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg
    10141 ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga
    10201 aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta actcttcgtt
    10261 agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt
    10321 ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact
    10381 acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa
    10441 aactttaata ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc
    10501 caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg
    10561 cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg ttatacgcat
    10621 attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc
    10681 ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact tagcttaaaa
    10741 aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg ctacatatcc
    10801 ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt
    10861 tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt
    10921 agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg
    10981 aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt
    11041 taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct
    11101 aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat
    11161 gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc
    11221 ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg
    11281 ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg
    11341 atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca
    11401 acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac
    11461 tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata
    11521 aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg
    11581 aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg
    11641 aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc
    11701 gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca
    11761 tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa
    11821 tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc
    11881 gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag
    11941 gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaacggc
    12001 gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt catcgtccgt
    12061 tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt ctatattacg
    12121 agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc
    12181 gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca gcgcgaggat
    12241 aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa
    12301 cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc
    12361 cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt gcattgggtg
    12421 taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag
    12481 cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac
    12541 tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc
    12601 accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctagc
    12661 ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt tgctggttaa
    12721 ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac cgcttaaatt
    12781 cagtgccata ttcagctgca ggttctgctt acgccagtcc ccttcgggta aagggatatg
    12841 cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt
    12901 aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa
    12961 gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa catcgaaatc
    13021 ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc
    13081 ttccacgttc tgattgccat caaccatcaa tgactcaggt aacaaaaaat ggataaaact
    13141 tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat
    13201 agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc
    13261 cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca ttatcggaat
    13321 caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa
    13381 catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag
    13441 ctatgtgaag tgacggtgaa gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg
    13501 cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc
    13561 gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag
    13621 cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc
    13681 cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg cgttatccag
    13741 taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggtggg
    13801 cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg
    13861 gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct
    13921 tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg tcagaatgtt
    13981 gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc
    14041 acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat
    14101 atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcag
    14161 ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg aatcagcata
    14221 gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag
    14281 tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc gtttaatgac
    14341 cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgcc
    14401 cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt
    14461 gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc
    14521 ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat cggtcatata
    14581 gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa acgggcgatg
    14641 ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc
    14701 gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct ccgttgctct
    14761 tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc
    14821 caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa gcccctcagg
    14881 ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc
    14941 ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc ctgcgcatct
    15001 tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac
    15061 gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc
    15121 tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag
    15181 aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac
    15241 ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca
    15301 cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac
    15361 aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc gggaacctgc
    15421 tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc
    15481 tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc
    15541 gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta
    15601 atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg
    15661 ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt
    15721 tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc
    15781 ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc
    15841 cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga
    15901 tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg
    15961 accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg
    16021 atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg
    16081 tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt ccgtcaagcc
    16141 ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta
    16201 tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg
    16261 ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc
    16321 tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag
    16381 atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg
    16441 tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact
    16501 ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc
    16561 gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca
    16621 caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga
    16681 gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct
    16741 ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc ctgaatgaaa
    16801 cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc gcgttagccc
    16861 aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat
    16921 atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat
    16981 acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat
    17041 actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc
    17101 tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc agatgcgaga
    17161 tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc tgataatcca
    17221 cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tacccatgct ggtaatccga
    17281 gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc
    17341 cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca
    17401 ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt
    17461 tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt
    17521 cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg
    17581 tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc tctttggcac
    17641 gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc
    17701 cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta
    17761 acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca
    17821 ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca
    17881 cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc
    17941 ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc
    18001 ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca ttgatggcga
    18061 cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg cgcgttcggc
    18121 aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg cgcttggtcc
    18181 cgtgagtcgc gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg
    18241 tttaccgccg ggaaactcac tcgcgccagc gtctatcacc aggcggtgat cctggcgctg
    18301 agtccgtttc acaatgccgt gtatcaggca ttgtaggacg gataaggcgt tcacgccgca
    18361 tccgacattc ggtgcacgat gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa
    18421 tcaatcgctt ttcagcaaca cctcttccag ccactgacgc agctccacgg gcgcggcttt
    18481 caggctgtta gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat tttttaactc
    18541 acgctgactc atttcgccac gcaacagctc ttcgacaata cgcacgcgag tccccaacgc
    18601 ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat cgttttggta
    18661 ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc
    18721 tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg ggaagcaaaa
    18781 tgactccccc ggttgtactc gctaaagagt acgatagcat atcataaacg tgcggatcag
    18841 taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat gaaatagcgg
    18901 tagtaagcat cataagccag cacgttcttc acataaccgc gcgtctcgga gaacggaata
    18961 ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt tgccaagcca ggttcgcacc
    19021 cgtccaggtc cggcgttata agctgctgag gagaaaatac ggttattgcc aaactgctga
    19081 taaacatatt gcaggtaact ggtgccaatg ttgatgttcg tttccggatc cagcaattgc
    19141 ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc tgtaccaggc
    19201 ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt ccaggcgctc
    19261 tcctgacggg caatcgccat cgcatagctt tgcgggatct ctttcccact ggtgtaacgt
    19321 ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca aagcttcccg
    19381 gcgatcgttg cctgaacgct aagatcccac cattggttat tgaaagcata ccgagccagt
    19441 tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct acgcgcggta
    19501 ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc ctgagtcagg
    19561 gtgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc accgatgcgt
    19621 tgtgctgcaa ccatcgggta gaaaccacgc tgttgtatga gttgatgcaa aatctctttc
    19681 gcttcagctt cacgtccgcg ttccagcaat aaatccgcct gccagtaacg ccattcatct
    19741 ttctcttttg cttccatcgg cagacgcgcc agccaggtat tcaggccgcg acgatcgccg
    19801 gtgccaagcg ccattcgcac acggcgttca atcaacgacg tggattgcga gcgcataatg
    19861 gcgtcatcgc gccatttcgc ctgctcatcg gtgacatcat tgcccatcaa acgccaggcg
    19921 acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcgcctg ggcaagcgac
    19981 gggatcatca gccgtgcatt ttcggcatcc tgccgcgcca cactggcaaa cgccaccgcc
    20041 gccatttgac gggtaaaatc ggtcgcgccg gttgtacgcg cgaaggtcag taccgtatta
    20101 gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc aggcatctgc
    20161 cctgccagca ctgttaccag gcctgtgtta cccgctttca tcgccagacg gatacgctct
    20221 aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa cttgtcacag
    20281 gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca ggcttcttca
    20341 ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt agttccgggc
    20401 ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag ttcattgacg
    20461 aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac aaagttagtg
    20521 accgtcaccg ccggttgatt catcaggtca tcggtgatct ggcggtattc caggtaggga
    20581 taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat ctgtcgatta
    20641 tcccaggcct gcttgatttg cgcgtaacgg ctacgctgct catccagtga gtcggctcgc
    20701 gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca ggtaacttgt
    20761 ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc gtcatgccgc
    20821 gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac acttttttac
    20881 ttttactgcg aaggtgatcg accaactggg attaggcggg gaaacaggct acacttcgcc
    20941 tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct caattcgttt
    21001 ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg aaaaacatct
    21061 ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt
    21121 ccaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc
    21181 agccagacat caagattggt tatctgccgc aggaaccgca gctgaacccg gaacacaccg
    21241 tgcgtgagtc cattgaagaa gcggtttcag aagtggttaa cgccctgaaa cgcctggatg
    21301 aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc gctgaacaag
    21361 gccgtctgga agagatcatt caggctcacg acggtcataa cctgaacgta cagctggagc
    21421 gtgcggcgga tgcgctgcgt ctgccagatt gggacgcgaa aatcgctaac ctctccggtg
    21481 gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc
    21541 tcgacgaacc gaccaaccac ctggatgccg aatccgtggc gtggctggaa cgcttcctgc
    21601 acgacttcga aggcaccgtg gtggcgatta ctcacgaccg ttacttcctc gataacgttg
    21661 caggctggat cctcgaactg gaccgcggtg aaggtattcc gtgggaaggt aactactcct
    21721 cctggctgga gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa gcggcgcgtc
    21781 gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc cgccagtcga
    21841 aaggtaaagc acgtctggca cgctttgaag agctgaacag caccgaatac cagaaacgta
    21901 acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa gtgctggaag
    21961 tcagcaacct gcgtaaatcc tacggcgatc gcctgctgat tgatgacctg agcttctcga
    22021 tcccgaaagg ggcgatcgtc gggatcatcg gtccgaacgg cgcgggtaaa tcgaccctgt
    22081 tccgtatgat ctccggtcag gaacagccgg acagcggcac catcactttg ggtgaaacgg
    22141 tgaaactggc atcggttgat cagttccgtg actcaatgga taacagcaaa accgtttggg
    22201 aagaagtttc cggcgggctg gatattatga agatcggcaa caccgagatg ccaagccgcg
    22261 cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgtgtt ggcgaacttt
    22321 ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc
    22381 tgctgctcga cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg
    22441 ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg ttcctcgacc
    22501 gtatcgccac gcacatcctg gactaccagg atgaaggtaa agttgagttc ttcgaaggta
    22561 actttactga gtacgaagag tacaaaaaac gcacgctggg cgcagacgcg ctggagccga
    22621 agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg cggcgcgaac
    22681 gccttatccg gcctacaaaa ccagcaattt caatacgttg taaaactgta ggcctgataa
    22741 gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatgcggc gtgaacacct
    22801 tttccggtct acaacagcct tagcatcctc aatttcgtgg cgggtcaccc gtgcaggttt
    22861 tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact catcaaaaaa
    22921 actcatttta tacctcgcct ttgagcagtt tcgtcacggt tatccttgct cccccatcat
    22981 ctcccgcacc agctccacgc agcgcaggaa acggctgtcg taatcgtcct cttcaacccg
    23041 cacgaattcg atattgttct cttccagcat ctccaccagc aagttctgga actctttgcg
    23101 atccaccgaa ctgccgaggc tgcgtaaacc atccgccacc cacggcgtgt tgttctccag
    23161 caggatcacc agatcgaaac ggtattcatc aatcagcgcc tgcacgaacg gatgctcacg
    23221 cccttcgtac tttttgcaga acgcctgggt agtgacaaaa tcggtgtcaa taaacgccac
    23281 tttattggcg tatttcactg caaaatcaat gtattgtgcg tggcccaacg cgattttatc
    23341 gtagtcggaa tactgcaatg cgatctcatc accgccgagg tgtgaaaaga cataatcgcg
    23401 gccatattcc cacgcactgg tggtgttgaa gatattggca agtttgttta ccagggtgga
    23461 tttaccgctc gactcgccgc caaggatcgc cacggtacgc acaaaaaacg gcttcacttc
    23521 ggtaggaata tattcccagt agcggaacgg gttttcgcgg atctgcgcac cgctgatact
    23581 cataaaggta cgtttcggat cgaccagcac cgtctcgatc cccagatgtt ccatatactg
    23641 tggcgcatcg gcttcttccg aggtgtagat caggtccggc tggatcccct tttcagccat
    23701 aaactttttg atgccgttgc tccacacatc ccagccatgc ggatacggct ccatgccctc
    23761 ttcgttgaaa gcatgaatac ggatattttt ctgatattta aaggtttgca atagccagcg
    23821 cagacgatcc ggcaccgtgg gctgctgcga catggcgctg tcttcaaaca acgcgcggtc
    23881 gcgggtatcg tcaaaaccca taatgatatg cagctcgtca acctggctac aggcgcgctg
    23941 gataaggtag atatgtccgg tatgcagcgg gtagaactta ccgaatacga caccaatggt
    24001 tttcttctgc cggggaaatt caagccccaa aaaacggtgc aatgcctcca gcttttgcgc
    24061 gctggggctt ttaattttgg cattcagtaa ctggcttaaa taccctttgg tcataccgct
    24121 ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg atggcagttt tcaggtaatc
    24181 aaatgacgac atatctccct ccgtatctct cattataagt cgtcgaacac gctaagcgcg
    24241 tcggagagtt ttttaacgcc aaaaatctgc atcccttccg gcgctttttt cggtacgtta
    24301 gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg cttcagaaat ccgctcctgg
    24361 ccactgggca ccgggcggat ctcccctgcc agcccgactt caccaaacac caccagatcc
    24421 tgtggcagcg gtctgtcacg caggctggaa accatcgcca gcagtaacgc taagtcgccg
    24481 ctggtttcgg ttaccttcac gccgccgacc acgttcacaa acacatcctg atcggccatt
    24541 tgcagaccac cgtgacggtg caagacagcc agcaggattg ccagacggtt ttgttccagc
    24601 cccactgcca cgcggcgcgg attcgccatt atcgagtgat cgaccagcgc ctgaatctcc
    24661 accagcaacg gacgcgttcc ttcccacacc accatcacgg agctgccgga ggtcacttca
    24721 tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt cacgcagccc ctgttcggtc
    24781 atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt ttttatggct gcgtaaggtg
    24841 cgaaaacggg agtcggcatc gccatccaga agcaccgaac agtcgataca gtgttccagc
    24901 actttcgggc cagccagcga accatctttg gttacgtgtc caaccatgac aatcgccaca
    24961 ccgcgcgttt tggcgaaacg cgtcagataa gccgccgttt cacgcacctg cgccacgcta
    25021 ccaggcgatg attgtacatc cgccatatgc atcacctgga tcgagtcaat taccatcagc
    25081 ttcggttgct cttcttcggc aatcaggcag atctgctcga tgctggtttc cgacaacata
    25141 ttgagattgt cagtcggcag gccaaggcga tgagcgcgca ttgccacctg ttgcagcgac
    25201 tcttcgccgg tgacatacag cgttttcatc tgctgggcca gtttgcacag cgtttgcagc
    25261 aacagcgtgg atttccccgc accagggtta ccgccaatca gaatggcact tcctggcacc
    25321 acgccgcctc ctagtacgcg gtcgaactct ttaaatccgg tggaaaaacg cggcagctct
    25381 tcaaggctga tatcggagag tttctggact tttgccaccc cggcgctacc ggcatagccg
    25441 ctgagacgct cgttacgcgc caccgttggc gacgcagcaa gacgcacctc ggtgatggtg
    25501 ttccaggcat gacaggcact gcactgcccc tgccagcgcg gataatcggc cccgcattca
    25561 ttacaaacaa aggcgcgttt tggagctttt gccacggtgt acctcgttaa tgctgtgccg
    25621 cccgcaggat gacgggcagg caattacttc tgattcaggc tgccagagag gatgcagaat
    25681 acccccatca ggtcagcgtg acggatggtg acttccgcct tttcattcac ttttggcttg
    25741 gcatggtagg caatccccag ccctgctgct ttgatcatcg gcaggtcatt agctccatcg
    25801 ccaatcgcca cggtctgcgc cagcgggatt tcatactcct gcgcgaggcg agtcagagtt
    25861 ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat tgccggtaaa tttaccgtcc
    25921 atgatctcca gttcattggc taccacggct gtcaggcgca gcttgtcgcg caggtattca
    25981 gcaaagaaag taaagccgcc ggaggcaatc gccactttcc agcccagcgt ttccagcttg
    26041 agcaccagct gcgttaagcc tggcatcagc ggcagatttt cacgcacctg ttgcagaata
    26101 ttggcgtcag cgcctttcag cgtcgccacg cggctgcgca ggctggcggt aaaatcgagt
    26161 tcgccgcgca tcgcccgttc ggttacctcc gccaccatct cgcccgttcc ggccagcttg
    26221 gcaatttcat caatacattc aatctggatg gcggtggaat ccatgtccat caccagcaaa
    26281 cccggcgtgc gcaggtgcgg gattttcccc agcggggcga catccagctg cgcttcgtgg
    26341 gccaggcgtg tagcccgtgc ggtgagtgaa cctgccagac gaatcacctg ataatcttcc
    26401 acgcaccagg cggcaacaat caccatcgcc gcacccagtt tgctctggta ttgggtcaga
    26461 cgctgtttat ccagcccacg accatacagc agccagccgc tacgacctgc gtggtaatcc
    26521 agtggcatca cttcatcacc acttaatgaa agaggcagac ccggccataa agagacatct
    26581 tcaggcaggt cgcaccaggt aatgttaggc attaaggctc ctgtaaaatc gttcgaagca
    26641 gggaaaataa cgcatgaggc taccttgtat ccattgcttc tggcaacatt aagtctcaaa
    26701 ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt ccggctacat cgggcagtga
    26761 ttgtcctgtt ctgtcttgcc ttgttagtgg cactgatgca gggggcgtca tggtttagtc
    26821 aaaaccacca gcgacagcgt aatccacagc tggaagaact ggcccgcacc ctggcgcgtc
    26881 aggtgacgct gaacgttgca ccgctgatgc gtaccgactc accggatgaa aaacgcattc
    26941 aggcgatcct cgatcagtta acagatgaaa gccgtatcct cgacgcgggt gtgtatgacg
    27001 aacaaggcga tcttatcgca cgttctggcg aaagcgtcga agtgcgcgat cggctggcgc
    27061 tcgacggtaa aaaagcaggc ggctatttta accagcagat tgtcgagcca attgcgggta
    27121 aaaacggacc gctcggctat ctgcgcctga cactcgacac ccatacgctc gccaccgaag
    27181 cccaacaggt ggataacacc actaacattt tacgcctgat gttgctgctc tcactggcaa
    27241 tcggcgtagt gctgacccgc acgctgctac agggtaaacg cacccgctgg cagcaatcgc
    27301 ccttcctgtt aaccgccagc aaaccggtgc cggaagagga agaaagcgag aaaaaagagt
    27361 gacccattac tacaagaaag gaaatcgtta tgtccacatt acgcctgctc atctctgact
    27421 cttacgaccc gtggtttaac ctggcggtgg aagagtgtat ttttcgccaa atgcccgcca
    27481 cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt agtaattggt cgcgcgcaga
    27541 acccgtggaa agagtgtaat acccggcgga tggaagaaga taacgtccgc ctggcgcggc
    27601 gcagtggcgg tggcggcgcg gtgttccacg atctcggcaa tacctgcttt acctttatgg
    27661 ctggcaagcc ggagtacgat aaaactctct ccacgtcgat tgtgctcaat gcgctgaacg
    27721 cgctcggcgt cagcgccgaa gcgtccggac gtaacgatct ggtggtgaaa accgtcgaag
    27781 gcgaccgcaa agtctcaggc tcggcctatc gcgaaaccaa agatcgcggc ttccaccacg
    27841 gcaccttgct actcaatgcc gacctcagcc gcctggcaaa ctatctcaat ccggataaaa
    27901 agaaactggc ggcgaaaggc attacttcag tgcgttcccg cgtgaccaac ctcaccgagc
    27961 tgctgccggg gctcaccatg agcaggtttg cgaggccata accgaggcct ttttcgccca
    28021 ttatggcgag cacgtggaag cggaaatcat ctccccggac aaaacgccag acttgccaaa
    28081 cttcgccgaa acctttgccc gtcagagtag ctgagagtgg aacttcggtc aggctccggc
    28141 attctcgcat ctgctggatg aacgctttag ctggggcggc gtggaactgc atttcgacgt
    28201 tgaaaaaggc catatcaccc gcgcccaggt atttaccgac agcctcaacc tcgcgccgct
    28261 ggaagccctc gccgggcgac tgcaaggctg cctgtaccgc gcggatatgc tgcaacaaga
    28321 gtgcgaagcg ctgttggttg acttcccgga ccaggaaaaa gagctacggg agttgtcgac
    28381 gtggatagcg ggggcggtaa ggtaactcgc tgattaaaat atccaaggaa ggaaatatga
    28441 tcgatctgga aaaccaggaa cgggagatta tcaatttaat gttcagccag cgtatttcct
    28501 ggctagccgc cgtgcgtatc aggcataaat tatcgcttgc tgaagtaagt aaaatgttag
    28561 gtatctcgat taattctcta aaacagatcg aaaagacgga acggcttagc tcaaacatca
    28621 aaagcaaaat ggcagaaatt tatggttgtc caccagagtt gctgatatgc ccttcctgga
    28681 tgacggctga acataaatga atagattcgt gaatagatac ttgaatgttt tttattttct
    28741 atccatatga aaaataatag taaatctata aatcagttgc tattgatgaa tagatactaa
    28801 aaagccggag cagtccccca ctccggctta acacgacaat cgcctttcag cgaaacacaa
    28861 ttactcttta tcgcccagca gaacggattc cagtgcgatt ttgatcatgt cgttgaaggt
    28921 ggtctgacgc tcagcggcag tggtctgctc gtgagtgcgg atgtggtcag acacggtgca
    28981 gatggtcagg gctttcgcgc caaactctgc agccacgccg tagataccag ccgcttccat
    29041 ttccacgccg aggatgccgt atttttccat cacgtcgaac atttcgccgt ccggagagta
    29101 gaacaggtca gcggagaaca ggttacccac gcgagcatca acgcccagtg ctttagctgc
    29161 atctactgcg ttacgcacca tgtcaaagtc agcgatagcg gcaaagtcat ggtctttaaa
    29221 acggatgcgg ttaactttgg aatcggtgca ggcccccata ccgataacga cgtcgcgcag
    29281 ttttacgtgc ggcagaactg cgccacagga accaacgcgg ataattttct tcacgccgaa
    29341 atcggtgatc agttctttgg tgtagatgga gcaggacggg atacccatac cgtgacccat
    29401 tacggaaatt ttgcggcctt tgtaagtacc agtgaagccc agcataccgc gaacgttgtt
    29461 cacttcacgg gcatcttcaa ggaaagtttc agcaatatac ttcgcacgca gcgggtcgcc
    29521 tggcatcaaa actacgtcag cgaaatcgcc catttctgca ttaatgtgtg gggtagccat
    29581 tgttttatcc tttttgtgac ataacaaagg cggagtcaac gctccgccca aatccatcag
    29641 aacatggctt tgccatattc catatcagaa gtaccaaaat attttgccag agtctggccg
    29701 atatccgcga aggtttcacg gtgacccagt gagcccggtt ttactttcgg gccatatacc
    29761 agtaccggaa tgtgttcacg cgtgtggtca gtaccggtcc aggtcggatc gcaaccgtgg
    29821 tcagcggtga ggatcaggat gtcgtcatcg cgcagcagag acatcagctc cggcagacgg
    29881 cggtcgaaca gttccagacc cgcagcataa ccggcgacgt cgcggcggtg gccccaggaa
    29941 gagtcgaagt caacgaagtt ggtgaagacg atggtgttat cacccgcttc tttcatctct
    30001 ttgatggtgg cgtcaaacag cgcgtccaag ccagtcgctt tcactttttt ggtgataccg
    30061 cagttggcgt agatgtctgc aattttaccg acagaaacca cctggccgtg tttttcatca
    30121 accagtttct gcagcacggt cggtgctggc ggctcaacag ccaagtcgtg acggttaccg
    30181 gtacgctgga agttaccagc tttgtcaccg ataaacggac gagcgataac acgaccgata
    30241 ttgtagccgc cgttggtcag ctcttcacgg gcgatttcgc acagttcgta gagtttatcc
    30301 agaccgaaag tttcttcatg gcaggcaatc tggaacacgg agtcagcgga ggtatagaaa
    30361 atcggcttgc cggttttcat gtgctcttcg cccagttgat ccagaatgac cgtaccggaa
    30421 gagtggcagt taccgaggta acccggcaga ttagcgcgtt cgaccagttt atccagcagc
    30481 tcttgcggga agctgttttc gtgatcggag aaatatcccc actcaaacag aaccgggaca
    30541 ccggcgattt cccagtgacc agacggggta tctttaccgg atgacatttc gtgcgcccat
    30601 gcgtacgcgc cgataacttc agcgttgccg tccattcccg ccggaatgaa accggtagaa
    30661 ccttcgtgtg ctttcgccag ccccagacgg gtcagatttg gcagattgag cggcccttta
    30721 cgaccattat cagcttcgcc tttggcacaa gcttctgcga tatgacccag ggtgtcagcc
    30781 ccgacgtcac caaagcgttc tgcatcttct gtagcgccga tgccgaatga gtccagcacc
    30841 ataataaatg cacgtttcat atgttctccg tactcagtgc ttcaaattta aatgatcaga
    30901 tcagtatacc gttattcgct gatacggcga tagacagttg gtgtgctttc cggtgcgtta
    30961 tcggcaagtt taattgccgc tttcaccgct ttcgccgctt cctgccagct gttttcgtct
    31021 ttcgcgagga taaccgccag cggacgctga ccgtctacct ggtcgcccag acgcgccata
    31081 tcagtaaagc cgacgctgta atcgatggtg tcagatgcct gacgacgacc gccgcccatt
    31141 gcaaccactg ccatccccag cgcgcgggta tccatttcac tgacaaaacc ttcggtatca
    31201 gcatagactg ctttcgtcag catcgctgtc ggcagatact tcgcgtagtt ctcaacgaag
    31261 tcggtcgggc ctttttgtgc cgctaccata cgaccaaaga cttctgccgc tttaccgttg
    31321 tccagcaccg cctgcaattt cgcgcgcgct tcggcgtcat ctttcgccag tttgccggag
    31381 atcagcatct ccacgcacag cgccatcgtg acatcaaaca gacgcgggtt acgatactca
    31441 cccgtcagga actgcaccgc ttcacgaact tcaaccgcgt tacctgcact ggaggccagt
    31501 acctgattca tgtcggtgag cagcgcggtg gtgcgcacgc cagcgccgtt agccacgcca
    31561 acaatcgctt cggcaagggc ttcagagagt tcgtaggtcg gcataaacgc gccgctaccc
    31621 actttcacgt ccatcaccag cgcgtccaga ccttccgcca gtttcttcgc caggatcgag
    31681 gcagtgatca gcgggatgga gtccacggtt gcggtaatat cacgggtcgc gtagaaacgt
    31741 ttatcagccg gagccagtga actggtctga ccgataatcg ccacgccgac gtctttaata
    31801 atttcgcgga aacggttgtc atccgggaaa atgtcgaagc cagggatgga ttccagtttg
    31861 tcgagcgtac cgccagtatg accgaggccg cgaccggaga tcatcggaat atagccgccg
    31921 caggctgcga ccatcggccc caacatcagc gaagtgacat cgccgacgcc gccggtcgag
    31981 tgtttatcaa caatcgggcc attcagatgc aggcttttcc agtcgagaac ggttcctgaa
    32041 tctcgcatcg ccatggtcag cgagacacgc tcaggcattg tcatatcgtg gaagaaaatg
    32101 gtcatcgcga gggcggcaat ctgcccttcg gaaatagtgt tgtcgcgaat accgttgata
    32161 aagaaacgaa tttcttcatc gctcagcgca tgaccatcac gttttttacg aataatttct
    32221 tgtgcgagaa acaaggtaac ctcccgagga aaagagtatg aggcggcttt tgccggatgc
    32281 gtctacgcct tatccggcct acgggtagat acgtctcgta ggcctgataa gcgcagcgca
    32341 tcaggcgtaa agcatcttac ttagtagctg ctggcgctct taccgtcgcc gtggcccagc
    32401 gctttcagca ggcttgccag caggctggaa gcaccaaagc ggtagtgacg cgcatcagcc
    32461 cagtcagcac cgaacagttc atctgcgatg gcgagatatt tctgcgcatc ttccgcagta
    32521 cgcacgccgc ccgccagttt gaaaccaacg gttttttcta cgcccatatc acggatcact
    32581 tccatcatga tgcgagcgct ttccggcgtc gcgttcacag ccactttacc ggtagaggtt
    32641 ttgatgaagt ccgcacccgc tttgatggag atttcagacg ctttacggat cagcgcttcg
    32701 tctttcagtt cgccggtttc gatgatcact ttcagcagta cattcgctgc cgcgcaagcc
    32761 tctttacagg ctttcaccag gtcaaaacca acctgctcgt cacccgccat cagcgcgcgg
    32821 tacgggaaca ccacgtcaac ttcatcagca ccgtaggcga ttgccgcacg ggtttctgcc
    32881 agcgcgattt cgatgtcgtc gttaccgtgt gggaagttgg ttaccgtagc gatacggatt
    32941 tccggggtgc cctgctcttt cagagttttg cgagcaatcg ggataaaacg aggatagata
    33001 cagatagcgg cggtattgcc gaccggagtt ttggcctgat gacacagagc aattactttc
    33061 tcgtcggtgt cgtcgtcatt cagggtggtc aggtccatca atttcagtgc acgcaggctg
    33121 cttgctttca gatcagtcat ttcattctcc tggcttgtcg ccaataaaat tcaccttgcg
    33181 agtttgttag tattctaaca tctactccgc aacacacttc gatacacatc acaattaagg
    33241 aaatctactt acaagtttgc atcactgtaa tgcgatctgg ttcaaataat tcactttcaa
    33301 atgaatgcgt cagtggtggc aaacgcatca ggatcaaagt gaacatcacg aaacttctta
    33361 caatggcgca tcaccttcgg cataaaagga aaagaagatg cccacttctc atgaaaatgc
    33421 actgcaacaa cgttgccagc aaattgtcac cagcccagtg cttagcccgg agcagaagcg
    33481 ccattttctg gcactggaag cagaaaacaa tctgccttac ccacagcttc ctgccgaagc
    33541 ccgccgcgcg ctggatgaag gtgtaatctg cgatatgttt gaaggtcatg cgccgtacaa
    33601 accgcgctat gtcttacccg attacgctcg ttttctggcg aacggttccg aatggctgga
    33661 gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc tttaccacca
    33721 cgtaccgtcg gtcacatcga tgccggtcta cctggggcaa ctggatgcgt tgttgcaacc
    33781 gtatgttaga attctaacac aaaacgagat cgatgttcga ataaaacgtt tctggcgtta
    33841 cctcgacaga accctgccag atgcctttat gcacgccaat atcggcccgt ctgattcgcc
    33901 cattacccgc gcgatcttac gtgcagacgc gcaggtttca ccgaacctga cctttatcta
    33961 cgatcctgaa atcacccctg atgacctgct gctggaagtg gcgaagaaca tctgtgaatg
    34021 tagcaaaccg cacatcgcca acggtccggt gcatgataaa attttcacaa aagggggcta
    34081 cgggattgtg agctgttaca actcactgcc gctggcgggt ggtggcagca cgctggtacg
    34141 ccttaacctg aaagccattg ccgagcgcag cgaatcgctg gatgacttct ttacgcgcac
    34201 tctaccgcac tactgccagc agcagatcgc catcatcgat gcgcggtgtg aattcctcta
    34261 tcaacaatca cacttctttg agaatagctt cctggtgaaa gaagggctga ttaaccctga
    34321 acgttttgtg ccaatgtttg gcatgtatgg gctggcggaa gcggttaact tgctgtgtga
    34381 gaaagaaggg attgccgcgc gctacggtaa agaagccgcc gcaaatgaag tgggttatcg
    34441 catcagcgcg caactggcgg agtttgtcgc caataccccc gtgaaatatg gctggcaaaa
    34501 acgcgccatg ttacacgcac agtcggggat cagttccgat atcggcacca cgccgggcgc
    34561 gcgtttgccg tatggcgatg agccagatcc gatcacccat ctgcaaactg tcgccccgca
    34621 tcatgcttat tattattccg gcatcagcga cattctgacg ctcgacgaaa ccatcaaacg
    34681 taacccgcag gcactggtac agctttgcct cggtgccttt aaagccggaa tgcgtgaatt
    34741 taccgccaat gtcagcggta acgatctggt tcgcgttacc ggttatatgg tgcgtttgtc
    34801 ggatttagaa aaatatcgcg ccgaaggttc acgcaccaac accacctggc tgggcgaaga
    34861 agccgcacgc aacactcgta ttctggaacg ccagccgcgc gtgataagcc atgaacagca
    34921 gatgcgcttt agtcagtaag attatcccct tctcctgcgt tgacgggcca ggcagtcgtc
    34981 tggctctgtt tttgcagggc tgcaatctgc gctgcaaaaa ctgtcacaat ccgtggacga
    35041 tgggacgttg caatgactgt ggggagtgcg tgccacagtg tccgcatcag gcgttgcaga
    35101 ttgttgacgg caaagtggtg tggaacgctg tggtctgcga gcaatgtgat acctgcctga
    35161 agatgtgtcc gcaacatgcc acaccaatgg cgcaatccat gagcgtggac gaagtgctta
    35221 gccatgtgcg caaagcggtg ctgtttattg aagggataac ggtaagcggc ggcgaagcca
    35281 cgacccaact gccgtttgta gtggcgctgt ttactgctat caaaaacgat ccgcaactgc
    35341 gccatctcac ctgtctggtg gacagtaacg gtatgttgag cgaaaccggc tgggaaaaat
    35401 tactcccggt gtgtgacggc gcaatgctcg atctcaaagc atggggtagc gaatgtcatc
    35461 aacaattaac cggacgcgat aatcaacaga ttaagcgcag catctatttg ctggcagagc
    35521 gcggcaagct ggcggaactg cgtttgctga tgatcccagg ccaggtggat tatttgcaac
    35581 acatcgaaga actggcggcg tttatcaagg gacttggtga tgttccggtt cgcctgaacg
    35641 cgtttcatgc ccacggcgtg tatggcgagg cgcaaagctg ggcgagcgcc acgccggaag
    35701 acgttgagcc gttggctgat gcattaaagg tgcgcggggt gagccggttg atatttccgg
    35761 cgctctattt gtgacatgcc ggatacggcc tacggttcgg cacagacttg taggcctgat
    35821 aagacgcgtc aagcgtcgca tcaggcattg tgcgccaact gccggatgcg gcgtgaacgc
    35881 cttatccggc ctacggttcg gcacaaacct gtaggcatga taagacgcgt taagcgtcgc
    35941 atcaggcatt atgcgccgac tgccggatgc tgcctacgaa gggcaaacta cccacgaacg
    36001 ttaaacaccg catacgtgtt attaagcaac acttcggcaa tctcatccgc gggttccggg
    36061 cgtaactcgc aaagcacggc aaacacacgg gcagcctgtt ccgggcggtt aggctgcccc
    36121 tgaaaaccgt tgagcggcat atccggcgca tcggtttcca gcaacaacga tgccagcggt
    36181 aattttgcga tgacatcgcg ggttttactg gcgcgtggat aggtgatagt accgcctacg
    36241 ccgattttgt agcccagctg tacaaaccgt tcagcctgtt gctggctgcc agaaaatccg
    36301 tggacaacac cagcacgcgg caaatcgtgg tgtttaagat gcatcgccag tttgtcgtgc
    36361 gtgcgccgtg aatgcaggat caccggcaga tcgtagcgtt tcgccagttt cagttgttcg
    36421 tcgagtaacc actgctgcct ctcaaattgc ggatcgtcgc cgaagagatc cagaccgatc
    36481 tcccccaccg ccaccacctt cgccggacgc ctttccagcg cctgctgtag ctgctcaaga
    36541 gacacatcgc tatgtttttc caacatacca ggatgcaagc ccaatgcggc atacagcggt
    36601 tgataatttt ccgctaacgc ctgcacgcgg gcaaaatttt ccgcctctgt cgccggaaca
    36661 atgatcttgc ctacgcccgc ttgtgccgcg cgttgcaggc tggcctcttc atcgccaata
    36721 aaaggcggga aatcaaaatg gcagtgagtg tcgataaaac ggcaaatcaa gccagatcct
    36781 cattgttaaa tgtcgggtcg ttcgcctgcg gagcgtcgct cacttcggca accagcgtat
    36841 cgttggcgac tggcgcaggc ggaatgacaa tcgattccgg cgtcaccact ggcaccagat
    36901 ggcgggtaag cggcgctttt tcagttaata gcttgccaac cgtggcgagg aaataacgac
    36961 cgcataaacg cccaagttta tagtcttcac gcagcgccgg aatccgactg ccaagcgcga
    37021 tactatgtaa tggcttcggc ggataaattt cggatatccg cagcttgccc ggtggtttct
    37081 caataaattg ctgaatgtca cgatagctgg tttcatgatg ctgcaccaga ttgaccagcg
    37141 gctgcaggct actgtcaccc agccagcgtt ccatgcgttt gaaccactgc ggcgtgtagt
    37201 acatttgtga cggcacagtg cggatgacga ccaacgtttt agcgccctgc cttgccgcct
    37261 ctttaaccgg aatcgcatca ctgatcccgc catccaggta gttaatgcct tccagcgaca
    37321 ctccgctacg ataaaagcca ggtatcgcac tggaggcgcg aatcacatcc agccagtttt
    37381 gtttggttgg taaaaagtaa ttcggctcgt agtcatcctg acgacaggcg cacatataaa
    37441 acgatttgcc gctgtcaaac aaccgcgcgg cggtatccat ttgcaacggc atctggcttg
    37501 cggtggcctc caccagccag tcgagatcga taagatttcc tccacggaca aagcgcaatg
    37561 gatcgaaaaa ttcgcgtttt gtggtatagc gcatgatgac tttgcgcgcg taaccgggct
    37621 gattgcagat aaacgccgag aggttctgcg ccccggcaga tgtgccgaga taaagatcga
    37681 aaggattaaa ctgcgcgcgc ataaactcat ccagcacgcc agccgtgaaa attccacgct
    37741 gtccgccgcc ttcgcacacc agagctattc gtccaggctg gaacggcctt agcgacaacg
    37801 gcgcaatatt accaagcgtt acaggtattc gctgccccac ttctgccttc ctgtttttgt
    37861 tgtgaaagaa agacaaagta acgcaattta ttattcgcta aaaccgcaaa agccagtccg
    37921 ctggactggc tttggataaa attttcttaa tggtctgaaa tctagggtcg ttttcggccc
    37981 atgaacaaac tcactaggaa cagaataatc ccgacgacaa agacaatttt agctgcgcct
    38041 gcagcggtac cggccagacc accaaaccca agtgcggcgg cgattaacgc gataaccaga
    38101 aatatgatgc cccaacgaaa cataagcgtc tcctttacca tagttaatgt caccgcttga
    38161 tatgagcgtg aaaatcactc aatgcgatat ttttagtgtg gtgcacatcg cgcctcccga
    38221 caaagttcgg gaggacgaat tacttagttt tcagatcatt tttaacgctt ttcacaccat
    38281 ctaccgcttt ggcgatactt tcagcacggt cactttgtgc ctgagaatct acggtaccgg
    38341 agagctgaac cacgccatcg gtggtttcaa ctttcacttt acgtgaaggg acgatatcgt
    38401 ccgccagcag tttggctttg atttcactgg tggtggcggt gtcacccgcg tagcccttca
    38461 ccgagccttc tttagcgtcg cgaacgtgca gtttgtcgct gacagaggtc acgccttcaa
    38521 cgcctttcgc cactttcact gcctcttcgg cctgggcctg actttcaacg aaaccgctca
    38581 gggtcacgac tttttgatcg gttttgacag agatatcggt gctcttgatg ttgtcatgat
    38641 ccaccagcgc cgccttcact ttcgcggtga tggcgctgtc atccatgaaa ttaccgactt
    38701 tattcataga gctatcgact ttttgccctg cgctttcatt ggtagtctgc gcgttgtttt
    38761 ccgcgtaggc agagccggtc gcgacggcag aggtcaacat tacagccagc agagttttcg
    38821 aaatcttcag tcttgtcata gtcatcgatt tattcctgta tgtttgctcg taatttgagc
    38881 ctagcaacac gaggttgcat tgctgaatag ggagagactt caccctctac agaagtcaat
    38941 ggtcgccatc acaaaagcga tgggtgatga ataacgacca ttacagcctc tgaatcagtt
    39001 attaatatcg gtagaatgac aatcgacggc tttagatact gatatctacg cattgaacgg
    39061 tatttaacgc catcagaaat gtcatcactt tgttaaatat agatcacaat tttgaaaccg
    39121 ctcgggatat cagcgaaaac ataagcaaaa gtgaatgttt taagaacatt ccgtaagcgg
    39181 ctaataagga agggaaattg acagggcgca gcggctaccg cgccctggag gcaagaatta
    39241 atgctcgcgg gtctggtgga actgaacgtc cggataacgt tcctgtgcca ggcgcaggtt
    39301 gaccatgctg gtagcgatgt aagcgaggtt atcgccgcca tcaagcgcca gttggctttc
    39361 gttcttacgc ttgaactctt cgaatttctt cgcgtctgca cattctaccc agcgggcagt
    39421 ggcaacgttg actgactcat acactgcttc aacgttgtat tcgctcttca ggcgcgctac
    39481 caccacatca aactgcagca caccaaccgc accaacgatc agatcgttgt tggagattgg
    39541 acggaacacc tgcaccgcgc cctcttcgga aagctgtacc agccctttga gcagctgttt
    39601 ttgcttcagc ggatctttca ggcggatacg acggaacagt tctggtgcga agttcggaat
    39661 accggtgaac ttcatcatct caccctgggt aaaggtgtcg ccgatctgaa tggtgccgtg
    39721 gttgtgcagg ccgaggatat cgcccggata cgcttcttca acgtgcgaac ggtcacccgc
    39781 cataaaggtc agcgcgtcgg agatcaccac atctttcgca gtgcgcacct ggcgcagttt
    39841 catgcctttt tcatatttac cggacaccac acgcataaac gccacgcggt cgcggtgttt
    39901 cggatccatg ttggcctgaa ttttaaatac gaagccggta aatttatctt cgctcgcttc
    39961 tacggtacgg gtatcggtct gacgcggcat cggcgcaggc gcccactcca ccaggccatt
    40021 cagcatatga tcaacgccga agttacccag cgcagtaccg aagaataccg gagtgatttc
    40081 gcccgcaagg aacagctctt tgtcgaactc gttagacgcg cctttaacca gttccagttc
    40141 gtcacgcagc tgctgtgcca gatcttcacc aaccgcagca tcgagatccg ggttattcag
    40201 ccctttaaca atgcggactt cctgaatggt gtgcccttta ccgctctgat agagataggt
    40261 ttcatcttta taaaggtggt aaacgccttt aaacagcttg ccgcagccaa ttggccaagt
    40321 gatcggtgcg cagccaattt tcagctcgtt ctcaacttca tcgagcagtt ccatcgggtc
    40381 gcggatatca cggtcaagtt tgttcataaa ggtgaggatc ggcgtgtcgc gcagacgggt
    40441 aacttccatc agcttacggg tacgatcttc aacacctttt gcggcgtcga taaccatcag
    40501 gcagcagtcc accgccgtca gggtacgata ggtatcttcc gagaagtctt cgtgccccgg
    40561 ggtgtcgagc aggttaacca ggcaatcgtg atacggaaac tgcatcacag acgtagtaat
    40621 ggagatccca cgctgctttt ccatctccat ccagtccgac ttagcgtgct ggttggaacc
    40681 acggcctttt actgtaccgg cggtctgaat ggcctgtccg aacagcagca ccttctcggt
    40741 gatggtagtt ttaccggcgt ccgggtgaga aataatggca aaagtgcggc gcttcgccac
    40801 ctctttcaaa taaggagaca acgtcataat tctttcttct tgagtaagcg gcatcgcgcc
    40861 gcgcttgttg aaaacgaaaa attgcggcta ttttacccat caacgggggg gaggcaatca
    40921 gtgtttacac aggagctgct ccagttcgtg caacgaagaa acggtccagg tgggcgcgat
    40981 gccttctggt tgctcgcgat tgtgcgcatt cagccagcag gtcgcaagcc cggcgttgat
    41041 gccaccgaga atatcggact cggcagtgtc gccaaccatc agcacgcgtg aacggtcagg
    41101 attgcccgcc tgttccagcg cataatcgaa aattttctta ttcggtttgg caacgccaac
    41161 ttcttcggaa atcaccagca aatcgaagta atcacgcagg cccgtccgtt ccagacgcac
    41221 ttgctgcaag gcgctaaaac cgttggtgat gatgccgatt ttagcgttgc tacgaatggc
    41281 gttaagcaga gaaaccgcgc ccggcaacgg cgtacagatt tccgccatcg cattaataaa
    41341 ggcatcgtta agcaagcctg cttcaacttt cagccgttcg gcccagctct cgaaacgccc
    41401 gtgctgaagc tgtaatgaag tgatcgcgcc gttttgataa tccacccaca gtggcttgtt
    41461 aacggcctga tagtcctgaa aatcttcagc ggtaaaggtg acgctgtaat caagaaacat
    41521 ccgctgcagg ccggtgaatg agtcaaaggt aaacagcgtt tcatcggcat caaagaaaat
    41581 ccagtcccac ttcattattc caccttgtct tacatactga ttggcaacgc catgatgatg
    41641 gcgtcttcgc gaccgtccgt ggtggggtag taattgcggc gaatcgtcgc ctcgttaaag
    41701 cctaaacttt cgtacagggc aatggcggca gcatttgagg cacggacttc cagccatagt
    41761 gtcgccacgc cgcgtttttc cagttcgtcg atcagatgtt ccagcagcgc cctacccagc
    41821 ccctgacgct gatagtcggg atcgaccgca atattgaaca atgtagcttc atccagcacc
    41881 acttgcgtaa tcgcaaacgc cgccattttg ccgttttgcg ttaactgaaa gttgagataa
    41941 cgctcgccct ggttgctggc aaaggttttt tcactccacg gaaaggagtg ggcgcgttgt
    42001 tcaatgtggt aagccgctgg taaatcagtc gtttcgaggg aagaaatcgt gttcatatgt
    42061 gcaaatttgt tgccataacg cggcgcgtgc cgttgggttt gcccgtaatt cggtgagcgc
    42121 cggtgatgcc acctgagcgc cttccaatga tagcggttcg tcagtaccca accgccagct
    42181 gttgcagcga ctgccttgcg gcagcatcgc gattttttct ggcgtcagtt gcagcacttg
    42241 atcggggctg acggttaatg cgcgcagaac atcgctcact aaaggatcag tcagggcggg
    42301 aagatcgttt gccaccatca ccagacggac gtgtgccggg ctcgcaatgg caatctcccc
    42361 ctgcaacgcg ccagggcgac gcagcgacca ctgggtaatg cccagttgct gtaactgcca
    42421 gtctcgtcgg gatgtcatag cgaatcgctc ctgttgtcag gggcgcaaat atagcaaatt
    42481 cgtcgatacc gcgccaacat atggctataa tcgccgccag tatcaattga ggagcattcc
    42541 atgtctgcat ttaccccggc aagtgaagtc ttgctgcgtc acagtgatga tttcgaacaa
    42601 agccgtattc tgtttgccgg agacttacag gatgacctgc ccgcgcgttt agataccgcg
    42661 gccagccgtg ctcataccca gcaattccac cattggcagg tgttaagctg ccagatgggg
    42721 gataacgccc gttttagtct ggtcgccacg gcgaatgatg tcgcagattg cgatacgctg
    42781 atttactact ggccgaagaa caaaccggaa gcccagttcc agttgatgaa tttactttct
    42841 ctgctgccag tggggacaga tatttttgtc gttggcgaga accgcagcgg cgtgcgcagc
    42901 gctgagcaga tgctggcaga ttatgcgccg ttgaataaag tcgacagcgc tcgtcgctgt
    42961 ggcctctatt ttggtcgtct ggaaaaacag ccagtatttg atgcggataa attctggggc
    43021 gaatacagcg tcgatggcct gacggtcaaa acgctgcctg gcgtgtttag ccgcgacggt
    43081 ctggatgtcg gtagccagtt gctgctctcg acgttaactc cgcacacgaa aggtaaagtg
    43141 ctggatgtcg gctgtggcgc gggtgtactt tcagttgcct ttgcgcgcca ttcgccgaaa
    43201 attcgtctca ccttgtgcga tgtctctgcc ccggcggtag aagccagccg cgcaacactt
    43261 gcagccaacg gtgttgaagg tgaagtcttt gccagcaacg tcttttccga ggtgaaaggt
    43321 tgttttgata tgatcatctc caacccgccg ttccacgatg ggatgcaaac cagcctggat
    43381 gcggcgcaaa cgctgattcg tggcgcggtg cgtcatctta atagcggcgg cgagctgcga
    43441 attgtagcga acgccttcct gccttacccg gacgtgctgg atgagacatt tggcttccat
    43501 gaagttatcg cgcaaaccgg gcgcttcaag gtatatcgcg ccattatgac ccgccaggcg
    43561 aagaaaggtt gagtatctga tatctgtcgc cgcataccgt gtcggatgcg gcgtgaacgc
    43621 cttatccgac ctacaaaacc tgcacgttag cccttcgtag gccagataag acgcgccagc
    43681 gtcgcatctg gcatctgccc ccgcatgcct accaaacctg cacgctagtt tcctgatgga
    43741 catttttcca gcaattacac atcaggcgat aattaactat tgacgaaaag ctgaaaacca
    43801 ctagaatgcg cctccgtggt agcaattctt tttaagaatt gatggtatgc gaaggtggcg
    43861 gaattggtag acgcgctagc ttcaggtgtt agtgtcctta cggacgtggg ggttcaagtc
    43921 ccccccctcg caccaaaaac cacgttgata ttgctcgcac tgggcgaagg tggcggaatt
    43981 ggtagacgcg ctagcttcag gtgttagtgt tcttacggac gtgggggttc aagtcccccc
    44041 cctcgcacca aacgaggcga tatcaaaaaa agtaagatga ctgtgcgaag gtggcggaat
    44101 tggtagacgc gctagcttca ggtgttagtg tccttacgga cgtgggggtt caagtccccc
    44161 ccctcgcacc aattatcttt acttcctttc ttgtttcttc cctgttttgt catccctgat
    44221 tcaaattcat tatcatcagc atgatgccag caataagcgc caggcacgat ggtagcaaaa
    44281 tgtaatgccg caactgtttg tgatacagca tcaccggcgt caatactaat cccatcagaa
    44341 tgacaatccg ccactgatcc ggcgataaat ccgcatacat cagccctgca atcagcaatc
    44401 ccggcagcaa cactccccag ccactgccca gcgtacgttg caacatggtt tcatctcttt
    44461 cattgataat gaaaaccaat atcatatgat aatttttatc atttgcaagc cagagaaatc
    44521 ccttgctatc gagtaaacct atcgctagga ttagcaatca ttatcattta gattactatc
    44581 acgattatgg cctatcgttc cgcaccgctc tatgaagatg tcatctggcg aacgcatctc
    44641 cagccgcagg atgctgggct tgcacaagcc gtacgggcga tgatcgccaa acatcgtgaa
    44701 catttgctgg agtttatccg cctggatgaa cccgctccgc ttaacgccat gacgctggcg
    44761 caatggtcat caccgaatgc gctaagttct ctactggcgg tctattccga tcatatctat
    44821 cgcaaccaac caacgatgat ccgcgagaac aaaccgctga tctcactatg ggcacaatgg
    44881 tatatcggcc tgatggtgcc accattaatg ctggcgctac tgacgcagga aaaagcatta
    44941 gatgtgtcgc cggaacattt ccatgctgag tttcacgaaa ccggacgcgt cgcctgtttc
    45001 tgggtcgatg tgtgtgaaga taaaaacgca acaccacatt cgccgcagca gcgaatggaa
    45061 acgttaatca gccaggcgct ggttccggtt gtgcaagcac tagaagcgac cggagagatc
    45121 aacggcaaac ttatctggag taataccggt tatttgatca actggtatct cactgagatg
    45181 aagcaactgc tcggcgaggc tactgttgaa tcgctgcgcc atgccctctt ttttgagaaa
    45241 acgctcacga acggtgaaga taatccactc tggcgtaccg tggtactgcg cgacggcctg
    45301 ctggtgcgcc gcacttgctg ccagcgttat cgcttaccgg atgtgcagca atgtggctat
    45361 tgtacgctga aatgaaagca ctgccgggga agtaaacccg gcatcatgcg gattaatagg
    45421 gatgcaacac attacttgct tcgcaatgcc gcatacataa cagaatatct gcggcctcca
    45481 acaaccctgc atcagaactg acgcccagtt tcgacatcac attaaattta tgcgctcgga
    45541 tcgttttgat attacgttta agctgctcgg caatttgtgt cattgagtag ccacgcgaca
    45601 taaagcgcaa tatttctctc tccgtcgggc ttaacgtccg gctttggtta atgtaccact
    45661 gattgttcag tcggtcggtc gcctgacgta caccatttaa tgacaaaaag agttcctgat
    45721 gaaaaatctc cagcgttgac gctttactta atacaccgtc cagcggtgat ggcgacaatg
    45781 aaccaatcag ccgagcttca atatcatcat ccacaataac taaacgccgg gtacgcggaa
    45841 acttaatcgc cagctcagtc aggcaagata atccctctct gcgctcactt ctcatggcag
    45901 aaaaagaaaa aatgaccgcc gcaaacgaga tccgcgacat ggcatcctga aatgactcct
    45961 gactggtata ggtatggagc ttataagggt tgcccgcaaa ctttctggat aaactctcaa
    46021 taccgatact actcatgacg catttttcta tgagtgcgac atttctcttc ttaattcggc
    46081 tgtgttccat tctgcggcat ctccataaac agaaggcaag ttaagttctc ttgcaccctg
    46141 cgcgctattt atccatgcat acatttcagc gttgctgtga attgccagtc gacgcatcgc
    46201 actgtttttt tgggcgctga tagttttatt acttttctta agcaatgaag ctatctggtt
    46261 gatgccccat cctttaccta atagccgcag taccttgcgc tctgaaagag tgagaatgac
    46321 ggagcgatag ctaaaatcat gagtttcagt cattgccgga gaggtcagca tggcgctgat
    46381 acgctctgcg tgcgtattgc cggaacgtac ggtcttgacc agattctcaa tgggttcaac
    46441 atcagacaat aacgtcgccg tagggcacat gagcagttct acagcctggg aataccagga
    46501 gcgtgacacc atgaaaaccc agtgaatttc ccgatattgt gagattaaag aataataatg
    46561 ttcacaaaca gaacgtggat cttcactttc accggctaaa tca
//
LOCUS       DAJVRP010000025        45805 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVRP010000025 DAJVRP010000000
VERSION     DAJVRP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.025"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            114..2267
                     /locus_tag="OQN12_001377"
     CDS             114..2267
                     /locus_tag="OQN12_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1653765.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="OQN12_001378"
     CDS             2320..4050
                     /locus_tag="OQN12_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653766.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OQN12_001379"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="OQN12_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1653767.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="OQN12_001380"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="OQN12_001380"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1653768.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="OQN12_001381"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="OQN12_001381"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1653769.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OQN12_001382"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="OQN12_001382"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1653770.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="OQN12_001383"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="OQN12_001383"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1653771.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="OQN12_001384"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="OQN12_001384"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1653772.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="OQN12_001385"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="OQN12_001385"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1653773.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="OQN12_001386"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="OQN12_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1653774.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OQN12_001387"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="OQN12_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1653775.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OQN12_001388"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="OQN12_001388"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1653776.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OQN12_001389"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="OQN12_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1653777.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OQN12_001390"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="OQN12_001390"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1653778.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OQN12_001391"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="OQN12_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1653779.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OQN12_001392"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="OQN12_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1653780.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="OQN12_001393"
     CDS             16308..16847
                     /locus_tag="OQN12_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1653781.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="OQN12_001394"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="OQN12_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1653782.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="OQN12_001395"
     CDS             complement(18206..18553)
                     /locus_tag="OQN12_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1653783.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OQN12_001396"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="OQN12_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1653784.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OQN12_001397"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="OQN12_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1653785.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OQN12_001398"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="OQN12_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1653786.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OQN12_001399"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="OQN12_001399"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1653787.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OQN12_001400"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="OQN12_001400"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1653788.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="OQN12_001401"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="OQN12_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OQN12_001402"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="OQN12_001402"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCS1653789.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OQN12_001403"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="OQN12_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1653790.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OQN12_001404"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="OQN12_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1653791.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OQN12_001405"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="OQN12_001405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1653792.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OQN12_001406"
                     /pseudo
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="OQN12_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="OQN12_001407"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="OQN12_001407"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1653793.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="OQN12_001408"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="OQN12_001408"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1653794.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="OQN12_001409"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="OQN12_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1653795.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OQN12_001410"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="OQN12_001410"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1653796.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="OQN12_001411"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="OQN12_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1653797.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="OQN12_001412"
     CDS             complement(40011..40466)
                     /locus_tag="OQN12_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653798.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="OQN12_001413"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="OQN12_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OQN12_001414"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="OQN12_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1653799.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="OQN12_001415"
     CDS             complement(41309..41986)
                     /locus_tag="OQN12_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1653800.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OQN12_001416"
     CDS             complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="OQN12_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1653801.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OQN12_001417"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="OQN12_001417"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1653802.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="OQN12_001418"
     CDS             complement(43192..43650)
                     /locus_tag="OQN12_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1653803.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="OQN12_001419"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="OQN12_001419"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1653804.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="OQN12_001420"
                     /gene_synonym="dgcC"
     CDS             complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="OQN12_001420"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1653805.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11910 a        11943 c        11204 g        10748 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaattgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgcc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttt cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caattttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccaag gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacac cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccagg cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtc gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc tagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat cagatggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagaact ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atcgtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtggcg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat agtcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aagcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttatt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactat ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gagatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    45541 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    45601 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    45661 cgccgccgct tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca
    45721 attttcccaa attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagccac
    45781 tttcgtagct cacccgttag cacta
//
LOCUS       DAJVRP010000026        40803 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVRP010000026 DAJVRP010000000
VERSION     DAJVRP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.026"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(181..858)
                     /gene="narI"
                     /locus_tag="OQN12_001421"
     CDS             complement(181..858)
                     /gene="narI"
                     /locus_tag="OQN12_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1653806.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(858..1568)
                     /gene="narJ"
                     /locus_tag="OQN12_001422"
     CDS             complement(858..1568)
                     /gene="narJ"
                     /locus_tag="OQN12_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1653807.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1565..3103)
                     /gene="narH"
                     /locus_tag="OQN12_001423"
     CDS             complement(1565..3103)
                     /gene="narH"
                     /locus_tag="OQN12_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1653808.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3100..6843)
                     /locus_tag="OQN12_001424"
     CDS             complement(3100..6843)
                     /locus_tag="OQN12_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1653809.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7236..8627)
                     /gene="narK"
                     /locus_tag="OQN12_001425"
     CDS             complement(7236..8627)
                     /gene="narK"
                     /locus_tag="OQN12_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1653810.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            8966..10762
                     /gene="narX"
                     /locus_tag="OQN12_001426"
     CDS             8966..10762
                     /gene="narX"
                     /locus_tag="OQN12_001426"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1653811.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10755..11405
                     /gene="narL"
                     /locus_tag="OQN12_001427"
     CDS             10755..11405
                     /gene="narL"
                     /locus_tag="OQN12_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1653812.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="OQN12_001428"
     CDS             complement(11406..12800)
                     /gene="ychO"
                     /locus_tag="OQN12_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS1653813.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            12985..13338
                     /gene="ychN"
                     /locus_tag="OQN12_001429"
     CDS             12985..13338
                     /gene="ychN"
                     /locus_tag="OQN12_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1653814.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="OQN12_001430"
     CDS             complement(13382..14077)
                     /gene="chaC"
                     /locus_tag="OQN12_001430"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1653815.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="OQN12_001431"
     CDS             complement(14235..14465)
                     /gene="chaB"
                     /locus_tag="OQN12_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1653816.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14735..15835
                     /gene="chaA"
                     /locus_tag="OQN12_001432"
     CDS             14735..15835
                     /gene="chaA"
                     /locus_tag="OQN12_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1653817.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16240..16347
                     /locus_tag="OQN12_001433"
     CDS             16240..16347
                     /locus_tag="OQN12_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1653818.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="OQN12_001434"
     CDS             complement(16496..17350)
                     /gene="kdsA"
                     /locus_tag="OQN12_001434"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1653819.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17386..18195)
                     /locus_tag="OQN12_001435"
     CDS             complement(17386..18195)
                     /locus_tag="OQN12_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCS1653820.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18199..18591)
                     /locus_tag="OQN12_001436"
     CDS             complement(18199..18591)
                     /locus_tag="OQN12_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1653821.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="OQN12_001437"
     CDS             complement(18588..19421)
                     /gene="prmC"
                     /locus_tag="OQN12_001437"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1653822.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="OQN12_001438"
     CDS             complement(19421..20503)
                     /gene="prfA"
                     /locus_tag="OQN12_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1653823.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="OQN12_001439"
     CDS             complement(20545..21801)
                     /gene="hemA"
                     /locus_tag="OQN12_001439"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1653824.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22015..22638
                     /gene="lolB"
                     /locus_tag="OQN12_001440"
     CDS             22015..22638
                     /gene="lolB"
                     /locus_tag="OQN12_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1653825.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22638..23489
                     /gene="ispE"
                     /locus_tag="OQN12_001441"
     CDS             22638..23489
                     /gene="ispE"
                     /locus_tag="OQN12_001441"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1653826.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23640..24587
                     /gene="prs"
                     /locus_tag="OQN12_001442"
     CDS             23640..24587
                     /gene="prs"
                     /locus_tag="OQN12_001442"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1653827.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24712..26391
                     /gene="dauA"
                     /locus_tag="OQN12_001443"
     CDS             24712..26391
                     /gene="dauA"
                     /locus_tag="OQN12_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1653828.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="OQN12_001444"
     CDS             complement(26446..26724)
                     /gene="ychH"
                     /locus_tag="OQN12_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1653829.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27002..27586
                     /gene="pth"
                     /locus_tag="OQN12_001445"
     CDS             27002..27586
                     /gene="pth"
                     /locus_tag="OQN12_001445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1653830.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27703..28794
                     /gene="ychF"
                     /locus_tag="OQN12_001446"
     CDS             27703..28794
                     /gene="ychF"
                     /locus_tag="OQN12_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1653831.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            29637..32504
                     /locus_tag="OQN12_001447"
     CDS             29637..32504
                     /locus_tag="OQN12_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1653832.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="OQN12_001448"
     CDS             complement(32604..34523)
                     /gene="dhaR"
                     /locus_tag="OQN12_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCS1653833.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            34751..35821
                     /gene="dhaK"
                     /locus_tag="OQN12_001449"
     CDS             34751..35821
                     /gene="dhaK"
                     /locus_tag="OQN12_001449"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCS1653834.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            35832..36464
                     /gene="dhaL"
                     /locus_tag="OQN12_001450"
     CDS             35832..36464
                     /gene="dhaL"
                     /locus_tag="OQN12_001450"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCS1653835.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            36475..37893
                     /gene="dhaM"
                     /locus_tag="OQN12_001451"
     CDS             36475..37893
                     /gene="dhaM"
                     /locus_tag="OQN12_001451"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCS1653836.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            38213..39910
                     /gene="treA"
                     /locus_tag="OQN12_001452"
     CDS             38213..39910
                     /gene="treA"
                     /locus_tag="OQN12_001452"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1653837.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(39989..40428)
                     /locus_tag="OQN12_001453"
                     /pseudo
     CDS             complement(39989..40428)
                     /locus_tag="OQN12_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10086 a        10576 c        10176 g         9965 t
ORIGIN      
        1 cttccttgaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
       61 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
      121 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgt
      181 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
      241 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
      301 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
      361 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
      421 gaacggaatg gtcagcaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
      481 atccgctccg gtagtggttg cacgcacgcg tgggctgaac atacgacgtt tcagcaaaag
      541 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
      601 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcaaca taccgaagaa
      661 gtgaccgaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
      721 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
      781 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
      841 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
      901 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
      961 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
     1021 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
     1081 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
     1141 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
     1201 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
     1261 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
     1321 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
     1381 ctgtactgga cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
     1441 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
     1501 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
     1561 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
     1621 tgaacagatt gaatttggta tctgagccgt ggcagccatc accaaaggta aagccgcagc
     1681 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
     1741 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
     1801 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacagtttca gcacgtttgt
     1861 aatgacgcat cgccagcata cgtttcagcg cacgcagtac cggtttggta tcaccggcgg
     1921 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaattc
     1981 cgttgctgcc cagctcgccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
     2041 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgctagt ttccattcca
     2101 tcgccatttt ataaaccggc gactgctgtg cagcttcaat aacgctcagc ggaataccgt
     2161 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacg tccagctgac
     2221 gctggtagag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
     2281 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
     2341 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
     2401 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
     2461 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
     2521 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
     2581 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
     2641 gtttatcaaa ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
     2701 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
     2761 gcgcggtatg caggttctga taatcaaaat cgaacggctc gtaataatcg tcgatccccg
     2821 gcagatgcgg gttagcaaag attttaccca gcagcatggc tcggttaccc atgcgcggtt
     2881 gcagtttgcc attgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
     2941 cagtcgggaa gccctggccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
     3001 cttcacggct ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
     3061 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
     3121 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
     3181 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
     3241 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
     3301 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
     3361 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gtgcaccgtt
     3421 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
     3481 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
     3541 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
     3601 gccgttggat ttctggccta tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
     3661 aaccagcagg ctttcaccga aatcacgcat ccactggtga tcctgataca gttgctgacg
     3721 accagagagc gtacgccatg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
     3781 aacgtgttca tcttccagac cagaccaagt cgggctggag ataattttgc gcggctgtgc
     3841 ctgaatatcg cggaagcgga tcttctcgtc ttctttattc agcgccagat gcgtatggtc
     3901 acgaccggta aattcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
     3961 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
     4021 ttttgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
     4081 ggtgttccaa gcaatccctt taccgccgtt accgattttc tccatcagcg ggccgataga
     4141 ggtaaagcgt tcgtaagttg ccggataatc gcgctctacg accataatgt gcggtgcagt
     4201 tttgcctggg atcaggtcgc attcgccttt tttccagtct ttcacatcca gcggctgcgc
     4261 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
     4321 atagccgacg cacacttcgg agaatttctt cgcgatggct ttgtagattt cccagtcgct
     4381 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
     4441 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
     4501 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
     4561 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
     4621 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
     4681 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
     4741 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
     4801 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
     4861 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
     4921 tccggtatag cgggatttgt ccgccatcgg tgacagcaac tcttccgccg tgacggtttc
     4981 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
     5041 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
     5101 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
     5161 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
     5221 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
     5281 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc
     5341 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
     5401 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
     5461 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
     5521 ttccgtgccg tcgccaccaa agtacgggaa gcctacctca gcgatctcat cctggctacc
     5581 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
     5641 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
     5701 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
     5761 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
     5821 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
     5881 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
     5941 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
     6001 gacggcaaca gttttggtgc ctttgtaacg cacttcagta aagaagtgag catccggggt
     6061 acgcgtctgc ggcacgtttg aaccccaggc gatgatgtag ctggagttat accagtcagc
     6121 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaggtcgca
     6181 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
     6241 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc
     6301 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccaaga
     6361 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
     6421 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
     6481 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
     6541 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
     6601 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
     6661 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
     6721 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
     6781 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
     6841 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
     6901 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaaga gtgatgggga
     6961 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt acagcataac
     7021 atgctgtaaa ttaagacgtt ttatacctgt agttatttaa actattaaga tttttatgca
     7081 aatgggtatt aaggagtatt ccccatgggt aacatattaa tggctcttaa tgaaatccgg
     7141 cgaaataagc catataacac taatatgttg taactaaatg acgcataaaa aaagcgcggc
     7201 atcatgccgc gcaaaggata atcaaatatt aagatttatt ttttagaatg ccgaccatat
     7261 accgcccaag taatcactac gcaggcgata tagaaaatca aaaatacttt cattgcacct
     7321 actggcgaac ccgttaatgc cagcgagcta ccaaacgctt tcgggataaa gaagccacca
     7381 atcgcgccaa tcgcagagat gaaacccagc gccgctgccg tgccggttgc cgcttcacgc
     7441 atcgcacgtt cgtcagaacc cccttctgct ttcacgcgat ccattgtcag tttacggaag
     7501 atcactgaaa tcatctggaa agtggaacca ctacccagcc cagctgtcag gaacagcgcc
     7561 aggaagaccg cgaagaacgc catgaagctt ccgccctgcc cgtcagtcgg taaggtcagg
     7621 aacagcaggc cgctgaaaat cgccatcaga ataaagttca ccagcgtgac acgagttccg
     7681 cccagacggt cagataatgc accacctgca gaacgcgcca gcgcaccaat aaacggcccg
     7741 aagaaagcgt attgcagaat ctgaacatcc gggaactgcg tttttgacag catcgcaaag
     7801 cccgcggaga agccgatgaa ggagccgaag gttgccagat acagcaggct cataatccac
     7861 agatgacccc gtttgagtac cggcaactgc tccttgatgg aggctttcga ggtagcaaga
     7921 tcgttcatgc caaaccacgc cgcaatggtg aagatggcaa ggaacggcac ccatatccag
     7981 gacgcattcg ccagatacag ctcagtccca tccggctgtt tgacgccctg gctaccaaat
     8041 actgcgaaaa tcgacagtga taccaccagc ggagcaacca actgcatgac gctgacgccc
     8101 atgtttccca gaccaccatt cagacccagc gcgccaccct gcttctgttt cggaaagaag
     8161 aagctgatgt ttgccatact ggatgcgaag ttcgcgccag caaagccgca tagcagagag
     8221 atgatgatga agacgctata aggcgtggag gtatcctgca cggcaaaacc cagccagacg
     8281 caaggaataa tcagaatacc ggtgctgaac gccgtccagc gacgaccacc gaagatagga
     8341 accataaagg agtatggaac acgtaataac gcgccagaaa ccgaaggcag cgcagtcaac
     8401 ataaatagct gatcggtcgt aaaattaaaa ccgactttcg gtaagttcac cgcaacagcg
     8461 ctgaacaaca tccatacgca aaacgccagc agcagacagg gaacggaaat ccacaggttg
     8521 cggctggcaa tacgttgacc gcgttgttgc cagaacgcag gatcttccgg tcgccaatct
     8581 gttatgacag ctccagtagc cctttcggag gcagatgagt gactcataga cacctctgat
     8641 actcgtttcg atgtctgcca ccttagtgtc tggagctaaa ggcaatttga tgtaaatcaa
     8701 acgataagat aactttatct atcattgata tttatcatta cccatagtga gtacagtgat
     8761 ttcataaaaa ttatgagatt tttcacggtg ctgtagaatc cctaccctta ccgatgtaaa
     8821 gcgactaacc acacggcaaa taaggagtaa ctctttccgg ctatgggtat acttcagcca
     8881 atagccgaga atactgccat tccagaatat atcgtcacat tcattaaggt tattgctcat
     8941 ttaaagcctg aaggaagggg tttacatgct taaacgttgt ctctctccgc tcaccctggt
     9001 taatcaggtt gcgcttattg tgttgctttc tactgctatt ggactggcag ggatggcggt
     9061 ttctggctgg ctggtgcaag gcgttcaggg cagcgcccat gcgatcaaca aagcgggatc
     9121 gctgcgcatg caaagttacc gtctgttggc ggcagtgcca ttaagcgaga aagacaagcc
     9181 cttaattaaa gagatggaac aaacggcatt tagcgccgag ttgactcgag cagcagaacg
     9241 agacggacaa ctggcgcaat tacagggttt acaagattac tggcgtaatg aactgatccc
     9301 tgcgctgatg cgtgcacaaa accgagaaac ggtgtcagcg gatgtcagcc agtttgttgc
     9361 cgggcttgat cagctggtat ctggttttga ccgcaccacg gaaatgcgta ttgagacagt
     9421 ggtactggtc catcgggtaa tggcggtatt tatggcactt ttactggtgt tcactattat
     9481 ctggttgcgg gcgcgactgc tacaaccgtg gcggcaactg ctggcaatgg cgagtgccgt
     9541 cagccatcgc gattttaccc aacgcgcaaa catcagcggg cgcaacgaaa tggcgatgct
     9601 tggaactgcg ttgaacaata tgtctgcaga actggccgaa agttatgccg tacttgagca
     9661 gcgggttcag gagaaaaccg ccgggctgga gcataaaaat cagatcctct cttttttatg
     9721 gcaggctaac cgccgtttgc attcccgcgc cccgctgtgt gaacgcctgt caccggtact
     9781 caacggctta cagaatttaa ccctgctacg tgatatcgaa ctgcgggtgt atgacactga
     9841 tgatgaagag aatcatcagg agtttacctg ccagccagat atgacttgtg atgataaagg
     9901 ctgccagctc tgcccgcgcg gcgtattacc cgttggtgat cgcggcacga ccctgaagtg
     9961 gcggctggct gactctcata cgcagtacgg tattttgctg gcgaccctgc cgcaggggcg
    10021 tcatcttagc catgatcaac aacaactggt ggataccctg gttgaacaac ttaccgccac
    10081 gctggcgctg gatcgccatc aggaacgtca gcaacagttg atcgtgatgg aagagcgtgc
    10141 caccattgcg cgcgaactgc atgattctat tgcccaatct ctctcttgca tgaagatgca
    10201 ggtgagttgt ttacagatgc agggcgatgc gctgccagaa agcagccgcg aactgttaag
    10261 tcagatccgt aacgaactga atgcatcctg ggcgcagttg cgtgaattgc tcaccacatt
    10321 ccgcttgcag ctcaccgagc ctggattacg tccggcgctg gaagcgagtt gcgaagagta
    10381 cagcgccaaa tttggcttcc cggtgaagct ggattatcaa ttgccgcctc gtctggtgcc
    10441 ttcacatcag gcaatccact tgttgcaaat tgcccgtgag gcattaagta acgccctcaa
    10501 acattcgcaa gcgagtgagg tcgtggtgac ggtggcgcaa aacgataatc aggtcaaact
    10561 gaccgtccag gataacggct gcggcgtgcc tgaaaatgcc atccgcagca atcactacgg
    10621 catgataata atgcgcgacc gtgcgcaaag tttacgaggc gattgccgcg tccgccgtcg
    10681 tgaatcaggt ggcaccgaag tggtggtcac ctttattccc gaaaaaactt tcacagacgt
    10741 ccaaggagat acccatgagt aatcaggaac cggctactat cctgctgatt gacgatcacc
    10801 cgatgctgcg aactggcgta aaacagctta tcagtatggc accagatatc accgtggttg
    10861 gcgaagcgag taatggcgaa cagggtattg aactggcgga atctcttgat cccgatctga
    10921 tcctgttaga tctcaatatg cccggcatga acggtctgga aacgctggat aaactgcgcg
    10981 aaaagtccct ctcagggcgc attgtggtat tcagcgtctc taaccatgaa gaagatgtgg
    11041 tcaccgcact gaaacgcggc gcggatggct atctgttaaa agatatggaa ccggaagatc
    11101 tgctgaaagc attgcatcag gcagctgctg gcgaaatggt attaagcgaa gcattaacgc
    11161 ctgttctggc cgccagcttg cgcgctaacc gtgccactac tgagcgcgat gttaaccagt
    11221 taaccccacg cgagcgcgat attctcaagc tgattgccca gggtttgccg aacaagatga
    11281 ttgcccgccg cctggatatc accgaaagca cagtaaaagt gcacgtcaag cacatgctga
    11341 agaaaatgaa gctcaagtct cgcgtggaag cagcggtatg ggtgcatcag gagcgcattt
    11401 tctgattacg gttcccagcg cagttcgtcg tttgacaatg catcgaacgg ttcgacaagc
    11461 gttagcgtga tctcattgga ggagacacgc tgcccctggt tatcttccac caccacagac
    11521 aatcgccagt gattgcttgc cccttccccg ttctgccagt caggcatgat cagcgtccag
    11581 ccctccgcgc tgttggcttg tgcgcccggc gtcaaactta atatctgcgt atcgccctgc
    11641 caaatcagtt gccgaatacc gtaacgactg cggatttgta atttcagcgg cactgtttcg
    11701 ccaggtttta gatcccacgg cggtgtcgcc agaaacaccg ttaacgtttt tcgctgtcgg
    11761 tactcaagag tcggtagatt atttcgctgc ggattgtcat agcgactacc acgtaacgac
    11821 tgactttcgg caacctcgcc cgcagaaagt tgttttttga gcggtacacc aaagcggtaa
    11881 ttaagattca gcccgaggtt attttgattt tcgccacttt ccccctgttt atgctgggcc
    11941 gtcacagtga ctaatggcac aggggtgtaa tttaatccca gactcaacgc gacgggattg
    12001 tgataacccg taccagagtt aaacaaatca acacgatcac caaaatactg ttctaggctg
    12061 acactggtat tgaggtgttg atagaacggc atgcgcatcc gagctgtcag gtcgtacccg
    12121 cgcgccatcc gttgttcctg cgtggctgtc tgttcatgcc atgcagcaaa cggctgataa
    12181 aagtttgccg ataatcgcaa atattcgccc cacgcttcgg caccaaagcc cgctcgctga
    12241 agattttcgt cctgcaagtt gtcataaaaa gtgttataac ccaccagcca gttgccgcgc
    12301 gcccagcgtt gcccaacgcc cacattgctc accaacccat tatcctgctg agtaagacca
    12361 agctggctcc aggtgagata acgatcatta tcttgtaacg gcacaaacca gcttccacga
    12421 ctgccggtga aatgtccttc gttatccact ttgacgtcaa cactggcatt tccccacggt
    12481 gatagccagg actctacgtg ctgattaacc tgttgactaa gcgcgtcgcg gacttttccc
    12541 aatgcgaaag cttttgcctg ctcgccagta tccagcccgt tatcattcat actggtttcg
    12601 ccgaaatctt tcacaatttc agcaaagtgt ttttccccat catgattttc gggagccatg
    12661 cctaaatccg gcagaccatc attgttatta tcaaagggat ttgccgcttt ttgctcgaag
    12721 gtagattgtg cgttagctgt accgcctgct acaagcaaga gtaaataaaa cgggataatg
    12781 cgaggaacga aacggctcaa aacgtcggta acttccggta ataatgaaaa accatagcct
    12841 taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca ggaaaatcct
    12901 taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga tgagtgtcta
    12961 attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa tggcgcacct
    13021 tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg agagcaggag
    13081 agcaatctgg atctgcgtct gttcctgatg tctgatgcgg tcacagccgg gttgcgcggg
    13141 caaaaaccag gggaaggcta caacattcag caaatgctgg agatccttac cgctcagaat
    13201 gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagcac acttcctctg
    13261 attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct gtcagccgat
    13321 aaagtgctca cattttaatg tttaaggggg cgtctgacgc cccactatga gggaagttta
    13381 ctcaggcgaa tcccggcctt aacacaccat ccggataatt ctccgccagt agttttttta
    13441 ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata agctcctgtt
    13501 ccagtgaaaa caggtattgt gcattggttc ccagcggacc gctcgccgcc gcaatcaacg
    13561 gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg tccataataa
    13621 acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag gttggcagat
    13681 aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc agcgtctctt
    13741 ctggtagtcg ataggcgacg cctgtggtgc gtccgccctc tttcagtgca agcatccgtc
    13801 ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg cgatgccatc
    13861 caaccagtgt accggtgcat gattcggtaa actccagtgc cggattccac atcaacgatc
    13921 cataaccgaa gatccacacc ggtccctcat caggtcgaca agccagcgtc gccgccagcg
    13981 aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca aatgccgttt
    14041 tacaatcggc attcatcaag aaatcacgcg ttatcacctt acacctccgc cactgcatac
    14101 ttccctgcga caactttttg gtgtcttccg gacattttta ccgctcattt tgaaaccact
    14161 aacggaacaa tatgcaatcc acggaccact tgcaaggcaa caccgcacga atagctttaa
    14221 ctaagctacc ggttttacga ttttttatgc catttatcat catccccttt ggcatattca
    14281 tgcttcacag cagcccaggc cactttatgc gcggtttctt cgcgactggc gtcatcacgc
    14341 cgatcttctt tatctttata ttgatcccat gcgctgttga acgcttcttt atagatatcc
    14401 tgggcatgag acggtagaac gtgctttacg ctttccggca gatcgctttt cgttttatac
    14461 ggcatcgttg actcctttta caaagttgaa cattaagtgt ggtcaacaat atctactctt
    14521 gccaaaacat aagaattttc atgtcagact gttcttaaga aacgctaaaa caaacattgt
    14581 ctaaacgtaa tacttatttt tatgccccaa tgagtaaaaa atcgacagcg gggtaaaaat
    14641 tcgtaaaatg ttaccgatat ccagacactt tctgttattt ttcctaccta ttttttacat
    14701 tttcgataaa ttcatctgca aagggataac cataatgtca aatgctcaag aggcggtaaa
    14761 aacccgccac aaggagactt cgcttatttt cccggttctg gtgctggtag tgctgttcct
    14821 gtggggaagt agccagacac taccagtggt cattgccatc aatcttcttg cgcttattgg
    14881 tattttaagt agcgccttta gtgttgtccg tcatgcggac gtattagccc atcgcctggg
    14941 agaaccttac ggttcgctta ttcttagcct ttcagtggtt attcttgaag tcagtttgat
    15001 ttcagcttta atggcaaccg gcgacgccgc gccaacgcta atgcgtgata cgctctattc
    15061 aatcattatg attgttaccg gtgggctggt tggcttttca ttattgttgg gcggtcgtaa
    15121 gtttgccacc caatatatga atctgtttgg tatcaagcag tatttaattg cgctgttccc
    15181 cctggcgata atcgtactgg tatttccaat ggctctgcct gcggcgaatt tttcaaccgg
    15241 tcaggcgtta ctggtagcat taatttctgc ggcaatgtat ggcgtatttt tgctgatcca
    15301 ggccaaaacg catcaaagtt tgtttgtcta cgagcacgaa gatgacagtg atgacgacga
    15361 cccgcaccac ggtaaaacgt ctgcccatag cagcctgtgg catgctatct ggttgattat
    15421 ccatctgatt gccgttattg cagtcaccaa aatgaacgcc agctcgctgg agacattgct
    15481 cgacagcatg aatgcccctg ttgcctttac tggcttcctg gtggcactgt tgattctgtc
    15541 gccggaaggt ttaggtgcgt taaaagcagt gttgaacaac caggttcagc gcgcgatgaa
    15601 tctgttcttt ggttcagtgt tagcaacaat ttcgcttacc gtacctgtcg tcacgctaat
    15661 tgcctttatg acgggtaacg aattgcagtt tgcacttggt gcgccagaaa tggtggtgat
    15721 ggtggcctct ttagtgctgt gccatatctc cttctccacc ggacgtacta acgtgctcaa
    15781 tggcgcagcg catctggcac tgtttgccgc ctatttgatg acgatatttg cctgacagtt
    15841 atatctcctc cgatgacgga aatcgggaga gataagataa attcctgctt attgtatttc
    15901 ttcatactac ctggacagct cgttaatcag aattgcgagc ctgtaccaca acctgcacga
    15961 aaccactgca acagctgttg aaaagccgga atgtactctt tattgcaaac atttcaggga
    16021 tgaagtgaag atgaattgag acgtagtatc cacaccaagc cggaattatc cggtgaggcg
    16081 caatgttgcg ggggctttat ccctggtggc attggttgct ggaaagagaa aacccccgca
    16141 cgttgagagg taaaacctga caacaaaacg ggggctaatc ttgaaaccag acacttcaag
    16201 aatagccgca aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc
    16261 atgactttct ggcacgacct ggcagcaccg atcctggcgg ggattattgc cgcagcgatt
    16321 gtcagctggt ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca
    16381 atatgcgccc gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt atctggcacc
    16441 catgaaaaaa gtcttaacgc agaacgctaa gactttattt ttcaagcaaa aaagattact
    16501 tgctggtatc cagttcttcg aaacctttca ccagatcatc aatcgctttc atctgcttga
    16561 ggaacggttc cagtttagcc agcggcagcg cggatggacc atcacatttc gcatgttccg
    16621 gatccggatg cgcttcaata aacagccccg ccagacctac cgccataccg gctcgtgcca
    16681 gctcagccac ctgagcacga cgaccaccgg aagctgcgcc aaacggatcg cggcattgca
    16741 gtgcgtgggt cacgtcgaaa atcaccggcg agttaccaga cactttcttc ataatgctga
    16801 agcccagcat atcgacaacc aggttgtcat agccgaagtt agcaccacga tcgcaaagaa
    16861 tcactttttc gttgccgcct tctttgaatt tatcaacgat attacccatc tgtcccgggc
    16921 tgacaaactg tggtttcttg acgttaatta ccgcaccggt tttcgccatg gcttcaacca
    16981 ggtcagtctg gcgagcaaga aacgccggca actgaatcac atccacgaca tcagcaacgg
    17041 gctgtgcctg acttggttcg tgaacgtcgg tgataatttt cacgccaaaa gtctgcttca
    17101 actcctggaa gattttcatc ccttcttcca ggcccggtcc acgataagag tggatggagg
    17161 agcggttggc tttgtcaaaa gaggctttga acacgtaagg gatacccagt ttctgggtca
    17221 cagttacgta gtgctcgcaa atgcgcatcg ccagatcgcg agattccagc acgttcatac
    17281 cgccaaacag tacgaacggc aggtcatttg ctacgttgat gtcgccaatg ctaaccactt
    17341 tttgtttcat aggatcgcct taatcttgag taaaatgtcg attaattaat gcagcacaat
    17401 atgtttatgc gcgatgttat ttatttgcgc acggatcatt tcgctgatcg ggtcttccgg
    17461 acactgttca acgaaataac ttaaatcgtt caacgcaacg tgttcgcaat ccagttgcgc
    17521 ataaatcaac ccgcgatcgc gaatttcata gggatcttca gggttgaatt gtaataaagc
    17581 ttcgctggtg cgtaacgcca gctccatctg attttcttcc atcaacgagg ctttgagtgt
    17641 atccagcaat ttgcggatta cctcaatgtt atcagcttca tcaaggtctt cataaaacag
    17701 ttccgccgac gggctgatat ttccctttaa ccatccgtcc agcatatgtt cgcttaacga
    17761 ttcaccgtta aaaggattaa tcagccaaat ttcgccatcc ggacattcaa tgcgcaatat
    17821 cagctgcgta gggaaaatca ccggcagcag cggcaaatcg agacgattcg cgacccataa
    17881 taaaaccgca cctaatgata ccgcactgcc ctgtcgattc tttaacacct ggtccagcca
    17941 taatgcatcg gaaagacgat aaacgccgcg tgaggcttta aatccccagt cgccgtaaaa
    18001 cagcgcgatc agtttttcca gctgctcttc taaaggcaga agctggctga tttcttcctt
    18061 cgccagacta acgagacgct ccagttcgtc gtaaacatct tgcgagggaa aatcgcggcg
    18121 gattgcttcg caagccagga tcatgccttc gcacaatggc gctttattaa attcgaaatc
    18181 agctaacgat ctcatgactt accccagtaa cggtactttt gtggtggcga gtttaatgat
    18241 gatgtacagc accaccaacg ccagcgggaa ggcaataatg cgcgcctgct gactgtgcat
    18301 acgacgataa tcgagtgcaa taaaacccaa aacgatataa atgataactc caaacagctt
    18361 ttcagtcagc cactgtgcct gcccggaaaa tggcaggatg tgcgctttag ccatcaacgc
    18421 aatgccgctt aacaataaca ccgtatcgac aaccggcggc acaatgcgcg tccagcgagc
    18481 aaatgcctgt ggatgcttct gataacgtag ccagaaacgt aaggttaata gcccaacaga
    18541 aagcgcgata ctaataagat gaacactaag cagtgtagaa aaacttgtca ttgataatag
    18601 cggccgagcg ttacgcgctc gttatcacca tagtcacggc aggtttcgac gtcatgatac
    18661 cccgcgagga taaatgcttg tcgcaccgct tcgccctgct gccagccatg ttccagaagc
    18721 agaaagccgc cggataccag cgcgttacgc gactgttcga tgatatgcac gatgtctgcc
    18781 attccactgt ctgccgcaac cagcgcagtg agcggctcaa agcggacatc gccttgttga
    18841 agatgtgggt cctgctcgtc aatatacggc ggattgctga caatcatcgc aaactgctgc
    18901 ccggctagcg cgctaaacca gtcgctttgc agaatgtgga tatttttgat cgccagatgc
    18961 tgggcattac gttgtgccag ggagacagca tcaggcatac gatctacagc gataatttcg
    19021 cagtccgggc gctcgctagc cagcgcaagc gcaatcgccc cggtacccgt cccgagatcg
    19081 agaatacggc aaggttgttc aggcaaccgc gccagtgcct gctccaccag acactccgta
    19141 tccgggcgcg gaattaaggt cgctggcgaa acaaataacg gcaacgacca gaattctcgc
    19201 accccggtta aatgagcaat gggttcacca tcgcgacgac gtgtcagtag cgcatcaagt
    19261 tgctgacatt gttcgtcagt cagctgcgtt tcaccaaagg cgagaataaa agtacgccct
    19321 ctgccggtaa catgctccag caggatttca gcatcacgcc gcgggctttc gctcgcctga
    19381 agttggctta ttgcttcacg taaccagtgt tgatattcca ttattcctgc tcggacaacg
    19441 ccgccagttg gtcggcctga tgttcctgga taatcggttc aatcagcata tccagcttac
    19501 cttccatcac ttcatccagg cggtagagcg tcaggttgat gcggtgatcg gtaacgcgcc
    19561 cctgcgggaa attgtaagta cggttacggt cgctgcgatc gccactcccc agcaggttac
    19621 gacgggtaga cgcttcggcc tgttggcgtt ttgccatttc agcagcgtgg atgcgagcac
    19681 cgagcactga aagcgccttc gctttgtttt tatgttgtga acgttcgtcc tgacattcaa
    19741 caacaatccc ggtcggcaag tgagtaatac gaattgccga atcggtggtg ttaacgtgct
    19801 gaccacccgc ccctgacgag cggaaagtat caatgcgtaa atctgctggg ttgatgtccg
    19861 gcagttctgc gtcaggcagt tctggcatta ccgcaacggt acaagcagaa gtatgaatac
    19921 gaccctgcga ttccgtagca ggaacacgtt gcacgcgatg accgccggat tcaaatttca
    19981 gacgaccata cacaccatca ccgctaattt tggcgatgat ctctttataa ccaccatgtt
    20041 caccctcgct ggcgctcatg atttctaccc gccagcggcg ggcttcggcg taacggctgt
    20101 acatacggaa cagatcgcct gcaaacagcg ctgcttcgtc gccgccggtt ccggcacgga
    20161 cttcgaggaa ggcgttacgt tcgtcatcag gatcttttgg tagtaataga acctgtaatt
    20221 gctgttccag ttgctcgctt ttttctttag cttcgcgcag ttcatcctgc gccatctcac
    20281 gcatttcagg atcgtcgagc atcatctgtg cggtttcgat atcttcctga acctgttgcc
    20341 agtcggtaaa acagcgcgaa acatcactta actgcgcata ttcgcgtgat aatgcgcgaa
    20401 aacgttcctg gtcggcgata gtttgcgcgt cacccagcaa cgcctgaact tcttcatggc
    20461 gttcatgcag ggcttccagt ttggcaacga tagaaggctt cataggcgta aatgcaccct
    20521 gtaaaaaaag aaaatgatgt actgctactc cagcccgagg ctgtcgcgca gaatattcag
    20581 gcgttcgtta tccccgtcac gggcggcctg ttgaagtgat ttcgttggcg catggatcaa
    20641 gcggttagtc agtttccatg ccagatcctg cataatggct tgcgcgtcgc cgccctgctc
    20701 aagggccgct aacgctttgg cggttaactc atcgcgaacg tgctctgcct ggctgcgata
    20761 ctcgcgaatg gtttcgctgg cgctttgtgc tcgcagccac gccataaatt cgctggtttc
    20821 ctgagcgaca atagtttccg cctcaaccgc tgcggcttta cgctgcgcca ggttgtgcga
    20881 aatgatgctt tgcagatcgt ccacactata aagataagca ttcgccagtt tgccaacttc
    20941 cggctcaaca tcgcgcggaa cggcaatatc caccaacagc attggttggt tacgacgact
    21001 ttttaatgcg cgctccacca tgcctttccc gataatcggt aacgggctgg cggtggaact
    21061 gatgatgata tcggcttcgc gcagacgttc gtcgatctca ctcagggcaa tcacttccgc
    21121 gccaacttca tctgccagaa tttgggcacg ttcgcgagtg cggttggcga taatcatctt
    21181 ctgtacttta tgttcgcgca gatgacgcgc taccagctcg atggtttcgc ccgcgcctac
    21241 cagcaacact gtgaccgtag agagcgattc aaagatctgc cgcgccagcg tacaagccgc
    21301 aaaagcgaca gacacagcac tggcaccgat atctgtttca gtgcgaacgc gtttcgctac
    21361 agagaaagat ttctggaaca tgcgttccag ttcgctggcc ttcatatgac ctttttgcga
    21421 atcgacaaac gcttttttaa cctgaccgag gatctgcggc tccccaagaa ccagtgaatc
    21481 caggccgctg gcaacacgca ttaaatggct aaccgcgtcg ttatcctgat gccagtagag
    21541 gcttttacgc agatcttctt cattaagatt gtgataatcg caaagccagc ggattaacgc
    21601 ctcttgcagg ttatcctgct cttcaacgct aagataaagt tccgtgcggt tgcacgtcga
    21661 cagcaccacg ccgccctgca ccatcggctg cgcaagcagg ctgtcaagcg cctgatcgag
    21721 cttatccggc gaaaacgata cacgttctcg cagcgataca ggtgccgttt tatggttgat
    21781 accgagtgct aaaagggtca tgtctgcggg aaataatacc aacgttgata gggttagtct
    21841 gcttgcatca tacaggatgc gtaagatcaa taaaagagag cgcccccttt tggagtaatt
    21901 gccgaaagcc gttagattct ggcaattaag acaacttgaa catagacgat agcggacggt
    21961 aacgctagca ttaagggtta taactgcaac gtatctcaag gacttgtcat cactatgccc
    22021 ctgcccgatt ttcgtcttat ccgcctgcta ccgctggctg ctcttgtgct cactgcctgt
    22081 tccgttacca cgcccaaagg tcctggcaaa agcccggatt cgccacaatg gcgtcagcat
    22141 cagcaagacg tgcgcaatct taatcagtat cagactcgcg gcgcgttcgc ttatatttct
    22201 gaccaacaaa aagtgtacgc ccgctttttc tggcagcaaa ccggccagga tcgctaccgt
    22261 ctgctgctca ctaacccatt gggcagcacg gaactggagc tgaatgctca accgggtaac
    22321 gtgcagttag tcgacaataa aggtcagcgt tataccgccg atgacgccga agagatgatt
    22381 ggcaaattga ccggaatgcc aattccgctc aacagcttgc gccagtggat tttaggttta
    22441 ccgggtgatg caaccgacta caaactggac gaccagtacc gcctgagcga aattacctac
    22501 agccagaatg gcaaaaactg gaaggttgtt tatggtggtt atgacaccaa aacgcaacct
    22561 gcgatgccag ccaatatgga actcaccgac ggtggtcaac gcatcaagtt aaaaatggat
    22621 aactggatag tgaaataatg cggacacagt ggccctctcc ggcaaaactt aatctgtttt
    22681 tatacattac cggtcagcgt gcggatggtt accacacgct gcaaacgctg tttcagtttc
    22741 ttgattacgg cgacaccatc agcattgagc ttcgtgacga tggggatatt cgtctgttaa
    22801 cgcccgttga aggcgtggaa catgaagata acctgatcgt tcgcgcagcg cggttgttga
    22861 tgaaaactgc ggcagacagc gggcgtcttc cgacgggaag cggtgcgaat atcagcattg
    22921 acaagcgttt gccgatgggc ggcggtctcg gcggtggttc atccaatgcc gcgacggtcc
    22981 tggtggcatt aaatcatctc tggcaatgcg ggctaagcat ggatgagctg gcggaaatgg
    23041 ggctgacgct gggcgcagat gttcctgtct ttgttcgggg gcatgccgcg tttgccgaag
    23101 gcgttggtga aatactaacg ccggtggatc cgccagagaa gtggtatctg gtggcgcacc
    23161 ctggtgtaag tattccgact ccggtgattt ttaaagatcc tgaactcccg cgcaatacgc
    23221 caaaaaggtc aatagaaacg ttgctaaaat gtgaattcag caatgattgc gaggttatcg
    23281 caagaaaacg ttttcgcgag gttgatgcgg tgctttcctg gctgttagaa tacgccccgt
    23341 cacgcctgac tgggacaggg gcctgtgtct ttgctgaatt tgatacagag tctgaagccc
    23401 gccaggtgct agagcaagcc ccggaatggc tcaatggctt tgtggcgaaa ggcgttaatc
    23461 tttccccatt gcacagagcc atgctttaag ccgggcaagc tgagtttcgg tgacaacgtc
    23521 accttgttcc agacgttgca tcgcgctctt taatacaccg cctggaaagg atcatgcctg
    23581 gcccgcacag ttttcggcag attctttcca ccaatggacg catgcctgag gttcttctcg
    23641 tgcctgatat gaagcttttt gctggtaacg ccaccccggt actagcacaa cgtattgcca
    23701 accgcctgta cacttcactc ggcgacgccg ctgtaggtcg ctttagcgac ggcgaagtca
    23761 gcgtacaaat taacgaaaat gtacgcggtg gtgatatttt catcatccag tccacttgtg
    23821 cccctactaa cgacaacctg atggaattag tcgttatggt tgatgccctg cgtcgtgctt
    23881 ccgcaggtcg tatcaccgct gttatcccct actttggcta tgcgcgccag gaccgtcgcg
    23941 tccgttccgc tcgtgtacca atcactgcga aagtggttgc agacttcctc tccagcgtcg
    24001 gtgttgaccg tgtgctgaca gtggatctgc acgctgaaca gattcagggt ttcttcgacg
    24061 ttccggttga taacgtattt ggtagcccga tcctgctgga agacatgctg cagctgaatc
    24121 tggataaccc aattgtggtt tctccggaca tcggcggcgt tgtgcgtgcc cgcgctatcg
    24181 ctaagctgct gaacgatacc gatatggcaa tcatcgacaa acgtcgtccg cgtgcgaacg
    24241 tttcccaggt gatgcatatc atcggtgacg ttgcaggtcg tgactgcgta ctggtcgatg
    24301 atatgatcga cactggcggt acgctgtgta aagctgctga agcgctgaaa gaacgtggtg
    24361 ctaaacgtgt atttgcgtac gcgactcacc cgatcttctc tggcaacgcg gcgaacaacc
    24421 tgcgtaactc tgtaattgat gaagtcgttg tctgcgatac cattccgctg agcgatgaaa
    24481 tcaaatcact gccgaacgtg cgtactctga ccctgtcagg tatgctggcc gaagcgattc
    24541 gtcgtatcag caacgaagaa tcgatctctg ccatgttcga acactaatcg aacccggctc
    24601 aaagacccgc tgcggcgggt ttttttgtct gtaatatcca tttgtatgac ctatgcctcc
    24661 ttcacctgcc atttagttga cagatgatgc gctcacggat gaaacattat tgtgaacaaa
    24721 atattttcct cacatgtgat gcctttccgc gctctgatcg acgcttgctg gaaagaaaaa
    24781 tatactgccg cacggtttac ccgtgacctg attgccggga taaccgtcgg gattattgct
    24841 atcccgctgg cgatggcgtt ggctattggt agtggtgtgg ccccccagta cggtttatat
    24901 accgcagctg ttgcggggat tgtcattgct ctaacgggtg ggtcacgctt tagcgtttcc
    24961 ggtccgactg cggcatttgt ggtaattctc tatcccgttt cgcaacagtt tggactggca
    25021 ggactgctgg ttgcgacctt gttgtcggga atctttttga ttctgatggg gctggcacgc
    25081 tttggtcgtc tgattgagta tattccggtt tccgtcacct taggtttcac ctcgggtatc
    25141 gggatcacca tcggtaccat gcagattaaa gattttctcg gtctgcaaat ggcccatgtc
    25201 ccggaacatt atctacaaaa agtcggcgca ttatttatgg cgctgccgac cattaatgtg
    25261 ggtgatgctg ccattggcat tgtgacgctt ggcattctgg ttttctggcc gcgtctgggc
    25321 attcgtttac ccggtcacct tccggccttg ctggctggtt gcgcggtgat ggggattgtt
    25381 aacctgctcg gcggacatgt tgctaccatc ggttcgcaat tccactacgt cctggccgat
    25441 ggttctcagg gtaacggtat tccgcaactg ctgccgcaac tggtgctgcc gtgggatctg
    25501 cctaattcag aattcacgct aacctgggat tctattcgca ccctgctgcc tgcggcattc
    25561 tcaatggcaa tgctcggggc gatcgaatct ctgctctgcg ctgtggtact ggatggtatg
    25621 accgggacta agcacaaggc gaatagtgaa ctggtcggtc aggggctggg gaatatcatc
    25681 gctccattct ttggtggtat taccgctact gctgccatcg cgcgttctgc cgctaacgtc
    25741 cgtgccgggg caacttcccc tatctcggcg gtgatccact ctattctggt tattctcgcc
    25801 ctgctggtac tggcaccact gctctcctgg ctgccgcttt ccgccatggc agccctgctg
    25861 ttgatggtgg cgtggaacat gagtgaagcg cacaaagtgg tcgacttgct gcgtcatgcg
    25921 ccgaaagatg acatcatcgt catgctgctg tgtatgtcac tgaccgtgct gtttgatatg
    25981 gttattgcca tcagcgtggg gatcgtgctg gcatcgctgc tgtttatgcg tcgtatcgca
    26041 cgtatgactc gcctggcacc ggtagtcgta gatgttccag acgatgtcct ggttctgcgc
    26101 gttattggcc cgctgttttt tgctgctgct gaaggcttat tcacggacct ggagtcacgt
    26161 cttgaaggca aacggattgt gattctgaag tgggatgccg ttccggtact tgatgctggt
    26221 ggtcttgatg cgttccagcg ttttgtgaag cgtctgcccg aaggatgtga actgcgcgtg
    26281 tgcaacgtgg aattccagcc actgcgaact atggctcgcg caggcattca accgatcccg
    26341 ggacgcctcg cgttcttccc gaatcgtcgc gcggcgatgg cggatttata agtacgagat
    26401 tgaccagtca gcgcaaactg actggtcagc aaactgcatt atttgttagc tatgacggcg
    26461 attatcgcta cggcggcaac gtttgtcata gtgacgaacc caccagtaac ggtcgcacac
    26521 ctgttcatgc ccgccaacac gggcacccgc cagccagaga atggcaccga cgaaaatact
    26581 tagcactgca ccatgtgcga aatactgggg catattaaac tgtggtaact ggttgaggat
    26641 tgaatacccc acgccgacca ccattaccac cagacccaac cccatgagca cgttaccgag
    26701 tatcgaagcg tttttgcgtt tcatatgtca cctccggaac tttctgggtt gtaacaggga
    26761 atacccctct tccttatgtg taaagtatag acaacaccct gtgggttatg tgcgggcgta
    26821 atcacaatta caacccttat ttcaacaaaa ctttacaaat aaacgcctga acaccttgac
    26881 tttctaatag tccactcagg caattattac gttatctgca tttgatacgc agttttttgt
    26941 caagcggggc cgcaaccagt aaactacgcg ccagttatgt acacactcag gacaaaaaaa
    27001 cgtgacgatt aaattgattg tcggcctggc gaaccccggt gctgaatacg ccgcaacgcg
    27061 acataatgct ggtgcctggt tcgttgactt actggcagag cgtttgcgcg ctccgctgcg
    27121 cgaagaggct aaattctttg gttatacttc gcgagtcacc cttggaggcg aagatgtccg
    27181 cctgttagtc ccgactacat ttatgaatct cagcggcaaa gccgttgcgg cgatggccag
    27241 ttttttccgc attaatccgg acgaaattct ggtggcccac gacgaactgg atctgcctcc
    27301 tggcgtcgcc aaatttaagt tgggcggtgg ccatggtggt cacaatggac tgaaagacat
    27361 catcagtaaa ttgggtaata accctaactt tcaccgttta cgcatcggaa tcggtcatcc
    27421 gggcgataaa aataaagttg tcggttttgt gttaggcaaa ccgcctgtta gtgaacagaa
    27481 gttaattgat gaagccatcg acgaagcggc gcgttgtact gaaatgtggt ttacagatgg
    27541 cttgaccaaa gcaacgaacc gattgcacgc ctttaaagcg caataagtcg ttgtctgcgg
    27601 catttttgcc gagtgccgtg tataataggc aaagttattt ccatttctgc aatctgttag
    27661 caataacagg ttgattatta agatattaag gtgatttaaa tcatgggatt caaatgcggt
    27721 atcgtcggtt tgcccaacgt cgggaaatct accctgttca acgcgctgac caaagccggt
    27781 attgaagcgg ccaactttcc attctgcacc attgagccga acacaggcgt cgtaccaatg
    27841 cctgaccctc gcctggatca actggctgaa atcgtaaaac cgcaacgtac gcttcccacg
    27901 accatggaat ttgtcgatat cgccggtctg gtaaaaggcg catcgaaagg cgaaggtctg
    27961 ggtaaccagt tcctgaccaa catccgtgaa accgaagcga tcggtcacgt tgttcgctgc
    28021 tttgaaaatg acaacatcat tcacgtttcc ggcaaagtta acccggctga cgatattgaa
    28081 gttatcaaca ccgaactggc gctggcagac ctcgacacct gcgaacgtgc gattcatcgc
    28141 gtacagaaga aagccaaagg tggcgataaa gacgcgaaag ctgagctggc ggtcctggaa
    28201 aaatgcctgc cccagctgga aaacgcaggt atgctgcgcg cgctggattt aagcgctgaa
    28261 gagaaagcgg ctattcgtta cctgagcttc ctgacgctga aaccaacaat gtacatagcc
    28321 aacgtcaacg aagatggttt tgaaaacaac ccatatcttg accaggtgcg tgaaatcgcg
    28381 gcgaaagaag gttctgttgt ggttccggtt tgtgctgctg ttgaagcaga cattgccgaa
    28441 ctggacgacg aagaacgtga cgagtttatg caggagcttg ggctggaaga accgggcctg
    28501 aaccgtgtga tccgtgccgg ttataagctg ctgaacctgc aaacttactt caccgctggg
    28561 gtgaaagaag tgcgtgcatg gaccattccg gttggagcaa ccgcgccgca ggcagcgggc
    28621 aaaatccata ctgattttga aaaaggcttt atccgtgcac aaaccatctc gtttgaagat
    28681 ttcatcactt acaaaggtga acaaggcgcg aaagaagcag gcaaaatgcg tgcagaaggt
    28741 aaagattaca tcgtgaaaga tggcgatgtg atgaacttcc ttttcaacgt ctaattaatt
    28801 tattgtctca tgaggtttcg ctaaatctca tgaagagcgc aaaagtatat aaaatccacg
    28861 caattgcgtg gatttttcat ttcaccgagt cctaatcatt cttgtaacat cctacacttt
    28921 tgtgcttatt gcctacagtg aaccacgaga ataattaaag acataagtgt tcagatgact
    28981 gatatctcat tagcagtgat ataacgtcag tttttgttcc actacacatc agccctcttc
    29041 acagctgatc aatattgatc tcgctccaat atgctggcga gaaaaggact tacctgaagg
    29101 gtaatatccc gcagataaag tccccgaata acgtgattac gtgcgatctt ggcaagatga
    29161 aaatgataac cagtagcaca ataataacga taggccagaa aatccattga acaaaaatga
    29221 atatcgatgt tttttgcttt ttcttattag taatgcaaaa atggcgcata ttgtttttgt
    29281 tatgcagata catcaagcga aaatcccata actaattagg attattacaa aaaatcttca
    29341 ccaaattttt taaccgctgt ttgttgtaat atttttgcca tattcatagt aacgaattgg
    29401 aataagtacg acaacttagg gaataactcg atataaactc acctttgtaa ggggggggtt
    29461 aaatatatat taattcctgt gttcctgatt ttgcccggta atagagaatt attctcagag
    29521 aagctcatca tgattgcacc atacagatat catgatgata agaggatatt attcattgac
    29581 ttaaacaaaa tgacaaaatc catagaccca tagcctgggt actatatagg agtggtatgg
    29641 gcatcaaaca acacaatggg aataccaaag ccgatcgtct cgctgaatta aaaatccgtt
    29701 cgccctcaat tcaactgata aaatttggcg ctattggttt gaatgcaatt atcttttccc
    29761 ccctgctgat agctgctgat acaggaagtc aatatggcac caatattact attaatgatg
    29821 gtgacagaat tacaggagat accgccgatc catcaggaaa cctctatggt gtaatgaccc
    29881 cagcaggaaa cacgcctggc aatatcaacc tgggtaatga tgtcaccgtc aatgtcaacg
    29941 acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa aaacagctcc ctgacagcta
    30001 accgactcac agtagatgtt gttggtcaaa cctctgccat cggcattaac ttaattggtg
    30061 actataccca tgctgactta ggcacaggca gcaccattaa gagtaacgat gacggcatca
    30121 ttattgggca tagctcaaca ctaacagcca ctcaattcac cattgaaaac tcgaacggta
    30181 taggcctaac catcaatgac tatggcacca gtgtcgatct tggaagcgga agtaaaatca
    30241 agaccgatgg aagtacaggt gtttatatcg gtggtctcaa cggcaataac gccaatggtg
    30301 ctgcgcgttt tacggcgaca gacctgacaa tcgatgttca gggctacagc gccatgggga
    30361 taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa cagtaccatt aaaaccaatg
    30421 gcgataatgc tcacggcctc tggagctttg gccaggttag cgcgaatgca ctcactgttg
    30481 atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg tggtacaacc actatcggtg
    30541 cagatagcca tatttcttcc gcgcagggcg gtggcctcgt caccagtagt tcagacgcga
    30601 caatcaattt ttctggcacg gcagcgcaac gaaacagcat cttttccggc ggttcttatg
    30661 gtgcctcggc ccagacggca acggctgtta tcaacatgca aaataccgat attacggttg
    30721 atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag cggcggtaga ataaccggag
    30781 acagtttggc tatcaccggc gcggcaggag ccagagggat ttatgccatg accaacagcc
    30841 agatcgacct cacgagcgat ctggtcattg atatgagtac acccgaccag atggccatcg
    30901 caacgcaaca tgacgatggt tatgccgcca gccgcatcaa cgcctcgggt cgtatgctta
    30961 tcaacggtag cgttctttcc aaaggtgggc taatcaatct ggatatgcac cctgggtcgg
    31021 tttggacagg ttcctccctc agcgataatg tcaatggcgg gaaactggac gttgcaatga
    31081 ataacagcgt ctggaacgta acaagtaatt ctaatctcga cacgctggcg ctgagccatt
    31141 caactgtcga ttttgccagc cacgggtcaa ctgccggcac atttaccaca ttaaacgtag
    31201 agaacctgag cggtaacagt acctttatta tgcgtgctga tgttgttggc gagggtaatg
    31261 gcgttaataa tagaggggat ttattgaata tcagcgggag tagtgctggt aatcacgtat
    31321 tggctatccg caaccagggc agcgaggcca caacgggaaa tgaagttctg acagtggtaa
    31381 aaaccactga cggcgcggcc tcgttcagcg cgtcttctca ggttgagttg gggggatatc
    31441 tgtacgatgt gcgtaaaaat ggcactaact gggagcttta cgcttccggg acagttccgg
    31501 aaccgactcc taatcctgaa cccacaccag ctcccgctca gcctcccata gtcaaccccg
    31561 atcctacgcc tgaacccgct cccacgccta aacccaccac gaccgcagat gctggcggca
    31621 attatctcaa tgtcggttac ttattgaact atgttgaaaa ccgtacgctg atgcaacgga
    31681 tgggtgactt gcgaaatcag agtaaagacg gtaatatctg gttgcgcagt tatgggggaa
    31741 gcctggactc ctttgccagt ggcaaactga gcggctttga catgggttac agcggtatcc
    31801 agtttggtgg ggataaacgt ctctctgatg taatgccgtt gtatgtcggt ctgtatattg
    31861 actcaacaca tgcatcgccg gactatagcg gaggcgacgg taccgcacgt tcagactaca
    31921 tgggaatgta cgccagttac atggcacaaa acggttttta cagcgatctc gttataaaag
    31981 catcgcgcca gaaaaatagt ttccacgtac tggacagtca gaacaacggc gttaacgcca
    32041 acggcactgc gaatggaatg agcatctccc tggaagccgg gcagaggttc aacctgtccc
    32101 ctactggtta tgggttctat atagagccgc aaacccagct tacatacagc caccagaatg
    32161 agatggctat gaaggcgagt aatggcctca atatacatct gaatcactac gaatcgctgc
    32221 tggggcgtgc cagcatgata ctggggtatg acatcaccgc aggcaacagc cagctgaatg
    32281 tctatgtgaa gactggcgct atccgcgagt tttcagggga taccgaatat ctgttgaacg
    32341 actcccggga gaagtacagt ttcaaaggta atggctggaa taacggcgtg ggagtcagtg
    32401 cacagtataa caaacagcac acattctatc tcgaagcgga ttacacgcag ggtaacctct
    32461 ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt ttaagtgcgg gatagacaag
    32521 cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat gaaatacaga tcactctgat
    32581 gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa actgccctgc atcaatgcca
    32641 tgttgcttca ttttccgcca cagcgtagtg cggccgatcc caagtaaagc cgacatttcc
    32701 tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg cctctttttc aacttccgca
    32761 aatgacagac tggtggaaag gcgggtggcg ctgacatcat ctgtcgcctg ctcggtaaac
    32821 agatgttcag gtaaatcact gacgcgaatg cgcccgttat cactactcag agcaagattc
    32881 tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg cacaagaaac taggcgagcg
    32941 agggcatcgt catcaatttt cagccgcgta gagaagcgtt tttcaagact gcgtaatttg
    33001 ttattcacca gcgccggaat gctgccacgc cgcatacgca gaggcgggat ggtaatttca
    33061 aatgcatgca gcgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg
    33121 aggtccacgg tcgttgttgc aatcactttg acatcaattg gtattaaacg ccgcgcatcc
    33181 agtcgcgtga taaccccctg cttgataacc tgcagcaaag cagactgtaa ctccaccgcc
    33241 agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg ccagttccag ccgactcaga
    33301 cggccatttt cattgtccgt gcgatcgcca ccaataaatt cttccgccag cgcagcatca
    33361 ccatataact cacaattgac ggcgatataa ggacctgcag cacgctcgct ttcattatga
    33421 attgcctgac ttagcagtgc cttgcccacg ccctcttctc cacaaagcag gacaggaaag
    33481 ctactgcgcg ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt
    33541 ggcatatgag cgaaggtatg gctgactttt cctaattgac tggtcattaa ctgccgcatc
    33601 tgttccacag gatggagcaa caaaataaag ctggttccct gcgtttctat tatcggttta
    33661 agggttatca ccgcatcaat aaactggtgc tggctttcaa aggttgcttc tacgtgtttg
    33721 agcggatgtg cctgttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg
    33781 attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcatta
    33841 ataaattgca aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac
    33901 agggcattaa gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt
    33961 aaattaccga cctcgcgggc gattgccaac gtcaacggca aatcagctgc ggtagtttgc
    34021 tcaaccggac acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc
    34081 gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc
    34141 acggcctgac cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg
    34201 caatacgtgc cgtcattgaa tcccagtgca cttagctgct gcaaggtttg cggatcgcca
    34261 ttacggctga gaatgcaggc ggtttcatcg aggataaaca gcgcacactc tcgcggtgcc
    34321 atatattccc aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc
    34381 cgataaatag aagcaaatgt cacgccctgg gcctgatgtg gtacgttcca tgtctcccgt
    34441 ttcatcagct tattgcatcg ctcccaggag gttgcaatta agggagatat gccccgacca
    34501 tcgttgttaa aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg
    34561 caattttaag tattaataca cgtaaaagtt tcaatatgga acattcatta aagcatatgt
    34621 ttcactatga aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt
    34681 catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg
    34741 gagcaaaata atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc
    34801 aggactggcg aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg
    34861 agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga
    34921 gccgatgcac tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat
    34981 tttcacctca ccgacgcccg ataaaatctt tgaatgctcc atgcaaattg atggcggcga
    35041 aggtgtactg ttgattatca aaaattacac cggcgatatt cttaactttg aaacagcgac
    35101 cgagttactg cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc
    35161 ggtaaaagac agtctttata ccgccgggcg acgcggcgtt gccaacacag tattaattga
    35221 aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg gaggcctgtg cggaactggg
    35281 gcgtaagttg aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc
    35341 tgccgcgggc aaaccttctt ttacccttgc ggataatgag atggagtttg gcgtcggcat
    35401 tcatggtgag ccaggtattg accgccgccc tttctcttcc cttgatcaaa ccgtcgatga
    35461 aatgttcgac accctgctgg aacatggctc ataccatcgc actttgcgtt tctgggatta
    35521 tcaacaaggc agctggcagg aagaaccaca aaccaaacaa ccgctccagt ctggcgatcg
    35581 ggtgattgca ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta
    35641 taaccgcctg accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg
    35701 cgcgtactgc acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga
    35761 cgaaacgctg gctctctggg acgccccggt ccacaccccg gcccttaact ggggtaaata
    35821 aggagaaagc aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg
    35881 atattttcaa caccgagagc gagtatctta ccgggctgga tcgcgagatt ggcgacgctg
    35941 accacgggct aaatatgaac cgaggcttta gcaaagtggt ggaaaaactc cctgctatcg
    36001 cagataaaga tatcggtttc attctcaaaa ataccggtat gacgctgctt tccagcgtcg
    36061 gtggtgccag tggtccgctg ttcggtacct tctttatccg cgccgcacag gcgacccagg
    36121 cacggcaaag cctgacactg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg
    36181 taatcagtcg cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg
    36241 tggtggaatc gttacgtcag tccagcgagc aaaatctctc tgttccggtg gcgctcgaag
    36301 ctgccagtag catcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag
    36361 gccgcgccag ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg
    36421 tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta
    36481 aacctggtca tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt
    36541 cagatgttaa tgagtgatag ttgtaaaatc gccattgccg cgggaattga cgatccacaa
    36601 aatcccattg gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgctgatgcc
    36661 gaccatgtgc tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg
    36721 gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa
    36781 ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg atatcgacaa agttatcttt
    36841 gacgctatgc atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact
    36901 gaaatctctg acacatgtcc tccgtacgat gaagaagccc gttctctggc ggtggtcata
    36961 aaaaaccgta acggcctgca tgtacgtccg gcctcccggc tggtttatac cttatcgaca
    37021 tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg tcacaccaga gagtattaac
    37081 cagattgcgt tactacaagt tcgctataac gatacgctgc gcctgattgc gaaagggcca
    37141 gaagctgaag aggcactgat cgctttccgt cacctggctg aagataactt tggtgaaacg
    37201 gaggaagtcg ctccacctac tctgcgtccc gttctgcctg tttcgggtaa agccttttat
    37261 tatcaaccag ttttatgtac ggtacaggca aaatcaaccc tgaccgcgga agaagaacaa
    37321 gatcgattac gccaggctat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa
    37381 gcagaagcca gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta
    37441 ggtgatccgg aactgctggc ggcggcaagc gaactccttc agcatgaaca ttgcacggca
    37501 gaatatgcct ggcagcaagt tcttaaagaa cttagccagc aataccagca actggatgat
    37561 gaatatctac aagctcgcta tattgatgtg gacgatcttc tgcatcgcac cctggtccac
    37621 ctgacccaaa cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag
    37681 aacatttatc cttccacagt actgcaactg gatccggcgg ttgtaaaagg tatctgcctt
    37741 agcgccggaa gtccggtatc ccacagcgcc ctaatcgccc gtgaactggg gattggctgg
    37801 atttgccagc agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac
    37861 gttaaaacgc aacgtttcaa ccgtcagggt taagccaccg ttcggatggc atcgttctga
    37921 tgtcatccgg caatttaccc agcactgatg ttttttaact acctgttata ttttttacca
    37981 atttatgatg atttttcttc gctaatcgcg aaccaaatgt cgctggctgg ctaataaaca
    38041 gacgttattt gatcactcta tttcgccgtt atttatccct ttaattccct tttctaaaat
    38101 gcctgaccgt tcgcagaatg agatttcgat catgcagcta gtgcgatcct gaactaaggt
    38161 tttctgatac ttgaataccg ttttattcgg tttcgccaaa ggagaatgat tgatgaaatc
    38221 ccccgcacct tctcgcccgc aaaaaatggc gttaattcca gcctgtatct ttttgtgttt
    38281 cgctgcgcta tcggtgcagg cagaagaaac accggtaaca ccacagccgc ctgatatttt
    38341 attagggccg ctgtttaatg atgtgcaaaa cgccaaactt tttccggacc aaaaaacctt
    38401 tgccgatgcc gtgccgaaca gcgatccgct gatgatcctt gctgattatc ggatgcagca
    38461 aaaccagagc ggatttgatc tgcgccattt cgttaacgtc aatttcaccc tgccgaaaga
    38521 aggcgagaaa tatgttccgc cagaggggca gtcactgcgc gaacatattg acggactttg
    38581 gccggtatta acgcgttcta ccgaaaacac cgaaaaatgg gattctctgt taccgctgcc
    38641 ggaaccttat gtcgtgccgg gcggacgttt tcgcgaggta tattactggg acagttactt
    38701 caccatgtta ggacttgccg aaagtggtca ctgggataaa gtcgcggata tggtggccaa
    38761 ttttgctcat gaaataaaca cttacggtca tattcccaac ggcaaccgca gttactattt
    38821 aagccgctcg caaccgccct tctttgccct gatggtagag ttactggcgc agcatgaagg
    38881 cgatgccgcg ttgaagcaat acctgccgca aatgcaaaaa gaatatgctt actggatgga
    38941 cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc gttgtaaaac ttcaggatgg
    39001 tacccttctc aaccgctact gggacgatcg cgatacgcca cgaccagagt catgggtgga
    39061 agatattgcc accgccaaaa gcaatccgaa tcgacctgcc actgaaattt accgcgacct
    39121 gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc tggatggaca acccgcagca
    39181 gttaaatacc ttacgcacca ccagcatcgt accggtcgat ctgaacagcc tgatgtttaa
    39241 aatggaaaaa atcctcgccc gcgccagcaa agctgcagga gataacgcga tggcaaacca
    39301 gtacgaaacg ctggcgaatg cccgtcaaaa agggatcgaa aaatacctgt ggaacgatca
    39361 acaaggctgg tatgccgatt acgacctgaa aagtcataaa gtgcgcaatc agttaaccgc
    39421 ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa gatcgcgcca acaaaatggc
    39481 gacggcgacg aaaacacatc tgctgcaacc cggcggcctg aacaccacgt cggtgaaaag
    39541 tgggcaacaa tgggatgcgc caaatggctg ggcaccgtta cagtgggtcg cgacagaagg
    39601 attacaaaac tacgggcaaa aagaggtggc gatggacatt agctggcact tcctgaccaa
    39661 tgttcagcac acctatgacc gggagaaaaa gctggtggaa aaatatgatg tcagcaccac
    39721 cggaacgggg ggcggcggtg gcgaatatcc attacaggat ggctttggct ggaccaatgg
    39781 cgtgacgctg aaaatgctgg atttgatctg cccgaaagag caaccgtgtg acaatgttcc
    39841 ggcgacgcat ccgaccgtta agtcagcaac gacgcaaccc tcaaccaaag aggcacaacc
    39901 cacaccttaa ccagcgctta ctccgacaac gtcattcggc ttctccggat ggcgttgtcg
    39961 gcagataaca ccatatctat ggttttttct acacagacgg tttttcaagt cctggcggga
    40021 agtattgcgc aaatgtttgc catacgtggt tgccgaagat agcgtcattt ctacgggtaa
    40081 gtgtttcact ttcatcaccc ggcgatttac gccgacgttg gctatataaa gcatcaattg
    40141 ctaccgaata ttgttacggt gttcactcat caacgttttt ttgctttctg gagttaaatt
    40201 aataatcaca taatccagac actccagaga atttagcaac caaaaaaaga attcagacca
    40261 ttcctcttcg cgtaactgaa gcattgccga acgggtattt tggcttaatc tgccagaagg
    40321 attgatcaat tctaaatgtt caataagcgc agccaaaacc gcctcactat ccgccccatt
    40381 tttaacgcct gactggctac tgtctaaaat atttattttc tgaatcacac caaaaacaac
    40441 gaaacaataa ttaaaaaagg attctgttgt tgttaccggg aaccgactta tttcgctcag
    40501 aaagtcgttc aaccagctcc gcagtgataa gattcgctga tgatacagtg tatcctcact
    40561 gcgtgctgga ggtgccagta taatatgttg atcaatacag tatttattgt gttcagtcgt
    40621 ttcctgatgc gtttggttct ctgcggattg tcgctggcga aataaatggc ttgcggtaaa
    40681 tgccgttaca gagcagcgca acgcgtcaat tccttcggag tgcccggctt ttggcgagag
    40741 ttccggtttt atgcgtccag gtagcggaaa tttcacccga atgagaaatg cattaataaa
    40801 atg
//
LOCUS       DAJVRP010000027        39761 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVRP010000027 DAJVRP010000000
VERSION     DAJVRP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.027"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..155
                     /locus_tag="OQN12_001454"
     CDS             <1..155
                     /locus_tag="OQN12_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1653838.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OQN12_001455"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OQN12_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1653839.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3035
                     /gene="uidA"
                     /locus_tag="OQN12_001456"
     CDS             1362..3035
                     /gene="uidA"
                     /locus_tag="OQN12_001456"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS1653840.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OQN12_001457"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OQN12_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OQN12_001458"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OQN12_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1653841.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OQN12_001459"
     CDS             complement(5626..7134)
                     /locus_tag="OQN12_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1653842.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OQN12_001460"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OQN12_001460"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1653843.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OQN12_001461"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OQN12_001461"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1653844.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OQN12_001462"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OQN12_001462"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1653845.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OQN12_001463"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OQN12_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1653846.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OQN12_001464"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OQN12_001464"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1653847.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OQN12_001465"
     CDS             complement(14283..15602)
                     /locus_tag="OQN12_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCS1653848.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OQN12_001466"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OQN12_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS1653849.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OQN12_001467"
     CDS             16699..17034
                     /locus_tag="OQN12_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS1653850.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OQN12_001468"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OQN12_001468"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1653851.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OQN12_001469"
     CDS             complement(17790..19172)
                     /locus_tag="OQN12_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1653852.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OQN12_001470"
     CDS             complement(19358..20302)
                     /locus_tag="OQN12_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1653853.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OQN12_001471"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OQN12_001471"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1653854.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OQN12_001472"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OQN12_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1653855.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OQN12_001473"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OQN12_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS1653856.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OQN12_001474"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OQN12_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1653857.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OQN12_001475"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OQN12_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1653858.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OQN12_001476"
     CDS             complement(25947..26768)
                     /locus_tag="OQN12_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1653859.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OQN12_001477"
     CDS             complement(26868..26951)
                     /locus_tag="OQN12_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653860.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OQN12_001478"
     CDS             complement(27044..27379)
                     /locus_tag="OQN12_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCS1653861.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27947..28204)
                     /locus_tag="OQN12_001479"
     CDS             complement(27947..28204)
                     /locus_tag="OQN12_001479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653862.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            complement(28220..29029)
                     /locus_tag="OQN12_001480"
     CDS             complement(28220..29029)
                     /locus_tag="OQN12_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1653863.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            29136..30056
                     /locus_tag="OQN12_001481"
     CDS             29136..30056
                     /locus_tag="OQN12_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1653864.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAKEPFVFFDPHVGTGLYDDI
                     LGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDA
                     VSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30191..31411
                     /gene="mlc"
                     /locus_tag="OQN12_001482"
                     /gene_synonym="dgsA"
     CDS             30191..31411
                     /gene="mlc"
                     /locus_tag="OQN12_001482"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1653865.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31536..32231
                     /gene="bioD"
                     /locus_tag="OQN12_001483"
     CDS             31536..32231
                     /gene="bioD"
                     /locus_tag="OQN12_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1653866.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32184..33476)
                     /gene="clcB"
                     /locus_tag="OQN12_001484"
     CDS             complement(32184..33476)
                     /gene="clcB"
                     /locus_tag="OQN12_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1653867.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33635..34249)
                     /gene="dmsD"
                     /locus_tag="OQN12_001485"
     CDS             complement(33635..34249)
                     /gene="dmsD"
                     /locus_tag="OQN12_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1653868.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34292..35146)
                     /locus_tag="OQN12_001486"
     CDS             complement(34292..35146)
                     /locus_tag="OQN12_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1653869.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35148..35765)
                     /gene="dmsB"
                     /locus_tag="OQN12_001487"
     CDS             complement(35148..35765)
                     /gene="dmsB"
                     /locus_tag="OQN12_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1653870.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35776..37814)
                     /locus_tag="OQN12_001488"
                     /pseudo
     CDS             complement(35776..37814)
                     /locus_tag="OQN12_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37875..38984)
                     /locus_tag="OQN12_001489"
     CDS             complement(37875..38984)
                     /locus_tag="OQN12_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCS1653871.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38967..>39761
                     /locus_tag="OQN12_001490"
     CDS             38967..>39761
                     /locus_tag="OQN12_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1653872.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSAS"
BASE COUNT        10122 a         9638 c        10179 g         9822 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaat actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgtaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggcatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtgg
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgccag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttacaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccacgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgtggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctag cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gctgaactgg cgaaagaacc ctttgtcttt tttgatccgc
    29761 acgtcgggac tgggctgtat gacgatattc tcggactgat gcgacgttac catttgacgc
    29821 ccgtcatcac tcaggaggtg ggcgaggcaa tgaccatcat cggtctggtt tccgccggtc
    29881 tgggtgtttc aattttgcct gcgtcattta aacgtgttca gctcaacgaa atgcgctggg
    29941 tgccgattgc tgaagaggat gcggtttctg aaatgtggtt ggtctggccg aaacatcatg
    30001 aacaaagtcc agctgcgcgt aactttcgta ttcatctgct gaatgctctc aggtgaggga
    30061 aatttcagcg aaaaagcccg aaaaatgtgc tgttaatcac atgcctaagt aaaaatttga
    30121 cgacacgtat tgaagtgctt caccatagcc tacagattat ttcggagcgc gaaaatatag
    30181 ggagtatgcg gtggttgctg aaaaccagcc tgggcacatt gatcaaataa agcagaccaa
    30241 cgcgggcgcg gtttatcgcc tgattgatca gcttggtcca gtctcgcgta tcgatctttc
    30301 ccgtctggcg cagctggctc ctgccagtat cactaaaatt gtccgtgaga tgctcgaagc
    30361 acacctggtg cgagagctgg aaatcaaaga agcggggaac cgtggccgtc cggcggtggg
    30421 gctggtggtt gaaactgaag cctggcacta tctttctctg cgcattagtc gcggggagat
    30481 tttccttgct ctgcgcgatc tgagcagcaa actggtggtg gaagagtcgc aggaactggc
    30541 gttaaaagat gacttgccat tgctggatcg tatcatttcc catatcgatc agttttttat
    30601 ccgccaccag aaaaaacttg agcgtctaac ttcgattgcc ataaccctgc cgggaattat
    30661 tgatacggaa aatggtattg tacatcgcat gccgttctac gaggatgtaa aagagatgcc
    30721 gctcggcgag gcgctggagc agcataccgg cgtaccggtt tatattcagc atgatatcag
    30781 cgcatggacg atggcagagg ccttgtttgg tgcctcacgc ggggcgcgcg atgtgattca
    30841 ggtggttatc gatcacaacg tgggggtggg cgtcattacc gatggtcatc tgctacacgc
    30901 aggcagcagt agtctcgtgg aaataggcca cacacaggtc gacccatatg gtaaacgctg
    30961 ttattgcggg aaccacggct gcctcgaaac catcgccagt gtggacagta ttcttgagct
    31021 ggcacagctg cgtctcaatc aatccatgag ctcgatgtta catgggcaac cgttaaccgt
    31081 ggactcattg tgtcaggcgg cattgcgcgg cgatctactg gcaaaagaca tcattatcgg
    31141 ggtgggcgcg catgtagggc gcattcttgc catcatggtg aatttattta acccacaaaa
    31201 aatactgatt ggctcaccgt taagtaaagc ggcagatatc ctcttcccgg tcatctcaga
    31261 cagcatccgt cagcaggccc ttcctgcgta tagtcagcac attagcgttg agagtactca
    31321 gttttctaac cagggcacga tggcaggcgc tgcactggta aaagacgcga tgtataacgg
    31381 ttctttgttg attcgtctgt tgcagggtta acatttttta actgttctac caaaatttgc
    31441 gctatctcaa tttgggccag gaaagcataa cttagacttt caaggttaat tattttccta
    31501 gtttatattt gtgaagcata acggtggagt tagtgatgct gaagcgtttc tttattaccg
    31561 gtacagacac ttctgtaggg aaaacggtgg tttcccgcgc attgctacaa gcgttagcct
    31621 cccagggaaa aagggttgcg ggatataaac ccgtagcgaa ggggagcaaa gagacacccg
    31681 aagggctgcg taataaagat gccttggtgt tgcagagtgt ttcaaccatc gaactgcctt
    31741 atgaagcagt taatcctatc gcgttaagcg aagaagaaag tagcgtggcg cacagttgcc
    31801 caatcaatta caccctcatt tcaaacggcc tggcaaacct gaccgataaa gtcgatcatg
    31861 tcgtggtaga agggactggc ggctggcgca gtctgatgaa tgatttgcgt ccactctctg
    31921 aatgggtagt gcaggaacaa ctgccggtgt tgatggttgt cggtattcag gaaggttgca
    31981 ttaaccatgc actgctaaca gctcaggcga tcgccaacga cgggctgccg ctcattggct
    32041 gggtggctaa ccgaatcaac ccaggactgg cgcattatgc ggaaatcatt gatgtgctgg
    32101 gtaaaaaact tccggcaccg ctcattggtg aactgcctta tctgccgcgc gccgaacagc
    32161 gtgaactggg gcaatacatc cgcttagcta tgctgcgcag tgtgctggcg gtagatagag
    32221 tcacggtgta acgtccgcga aattaccgac gcaattacgc aggcaatcaa taaaccgggg
    32281 agtagctgat actccccggt catttcacat atcatcaacg tcgacataat cggcgcgtgc
    32341 gtggttgccg ccaacagtgt cgccatcccg gtcaatccga gtaaaagcgt aatttcttcg
    32401 ccatcaggga accataatcc caggctacga ccatacaaca tgccaatggc aagaccgata
    32461 aatagcgtcg gtgtaaagac accaccgggt gcgccggaac cgctactcgc cagcacggca
    32521 cacagtttac aaaggaagat cccggcaatg atcattaaca gtggtggggc ggttaaaaag
    32581 gattgtacgg tgctatagcc gttgccccac actgcaggtg tgaacaggga aagcagaccc
    32641 acgatcaacc cgactagtgc cagttgccag ggcggcgcaa gtttgagact cacaaatcca
    32701 cgatgacagg cgttcattaa cgttaacaac agtggtccgc acagacctgc cagcacacct
    32761 gtactgataa tcagcgcata gtcacgagcc tgaaccgtca ctgagagttg tacgttgtag
    32821 agtaacgcgt cgctatgatt aatcagattg ctaaccagcc atgccacgat ggcggaaata
    32881 atcaccgggc cgagagaggc caacatcata gtgccaaaca gcacttcggc tataaataaa
    32941 ctgccagcaa gcggggcacg ataggccgca gccattcccg ccgcggcccc acaggcgatc
    33001 cataatttcc attcctggcg tggcgtaaaa cgttgggcaa aacaggaggc ggcaagggca
    33061 gctaaaagaa tcatcgcacc ttcgcgacca attgcactgc cgctggttac taccagcagc
    33121 gaggcaagcg atttaaccag gcttgctgcg taatcgaact gtccatcggt ttgcaacgct
    33181 tccatgtaat cggtcggcgc atgagggcgt tgttgggtaa atttctgcca gcccatcagc
    33241 aacaaacccg ccgccagtcc gccgagcgcc ggagttagca gccgtcgcca ggaggaaagg
    33301 tttgtcgctg cattgaccag actgccggag tcattattga ggaacaacca ctccagtagc
    33361 agcatcgcat gacgaaaccc ggcaacggca aagacagcga gaataccgac gactgttgcg
    33421 ataagcagac ggcggaacat agtgcgcaga tccggataga tgtgtagatg atgcatgagc
    33481 agaaatgggc aatgaaacac gggcgactat tttgcggcaa aagaggccaa ccgcaaaagc
    33541 agtgcgtgct ttaagagata aaaaaacgtg acattgtaac gtattattgc cgaatcgcgc
    33601 gtaatcgtca ccatccggca atattacggt gatcctatcg aaagagtggt ttaaccgcga
    33661 caggaatcaa cagttgtgat tgccattgcg ccagcgttaa gcgagccagt tcacccagtg
    33721 cacggtagaa agggtgttct gctttttcga taaaaacatc aagaaaacgt gttgaccacg
    33781 gaaaaaggtg ccatgccagc aattcttcgc attctgtctg gcgaccattc tccgccaacc
    33841 acgcagccat caacagcagt gaaccaaaat gatcttccgg ttcgttttgc ttcatttcaa
    33901 actgaatgcc tttctcgcgc atccactgac gaagtgccaa tgttgaatcg ccaaacagca
    33961 cagattcgcg atccagccag accgaccccc acggcggaga cggcagtgcc cacgggccga
    34021 caaacaaacg ctgccaggcc tgggcgtgag tctcttcaca ctgtgtctga aaagccgtca
    34081 ccagcggcgc taatgacgct tctggtagag gccactgagt ttcccagccg tcactggtga
    34141 gtaccgcaac cagaggtgcg gcttccgcac tctctggagc gtaataaaac agcgcgccca
    34201 acacgcgcgc cgcgacagaa aaattatctt gctgtgaaaa atgggtcatt cctaacatcc
    34261 tgaaatgtgc gggttgcccc gcaccaattt gttaacctgc aatagccatt cctactgtca
    34321 tgtgcaggcc ataaaagagc acacgcccga tcatctcacc aacaagtatg aggattagcc
    34381 ccagaacaag tccggcaacg tgaggttcac ggcgacgaat gagcggacaa agccagcatc
    34441 caagacctgc gcataacaat accacacgcc agacctgtaa ggaggcgtaa tctggtacca
    34501 gtgcgctggc ttgttgcacg gatgagtgaa tagacgccag agacaaacct tgcagcacaa
    34561 tcacctcggc acaagcgatc agcgccagaa cgctaatgat ggcaaatggt gtggtattaa
    34621 atgtaacgcg ggctgcccgc agaattgcgg cagcaagtat tggaccgctc aacaataccg
    34681 tcaggaagaa agccagcgta gtgtaaccgt tatgccaggt tggcacggtg tcgatttgat
    34741 acacgcaggt catcatccag acgaaaatga cgccgagcgc catgctgaca agtaaccaga
    34801 gtttccccaa cgcttgcggc attttaccga tgaccgccac cagccaccat agtccgccaa
    34861 cggcaaaaaa tatggaaccg gcagcaatct cgttactcag accagaagct ccgattcgat
    34921 tcagcgagtt aaacgctcgc agtggcgatc ccaggtgcat gacagaggca ataaacccga
    34981 tgcccatcaa cagccagagg aaaaacatgc cacgaacaat gtgctgacgg tctgcgtcat
    35041 ttttggcggc gaaccagcca attccgctaa caatcagcgc gcctactacg cattgaccca
    35101 atacggtaaa gataaccagt ggccattcat gccatccatt tcccatttta cacttcctcc
    35161 ggattagcca gataaccagt ggtatcgccg gtcgggcggc tgttggcgtt aggtttgata
    35221 acgatattgg gttttgtgaa atgcgcgcgc ggcagtggcg cgacggcagc cagagtgccg
    35281 tgcttctggc gcagctcttc aattggaccg aactcaagtg cacgcagcgg gcaggattcg
    35341 acgcatatgg gttgtttccc ctcggcgacg cgcgaataac aaccatcgca cttcgtcatg
    35401 tgcccttttt cagcattgta ctgtggtgcg ccgtacggac aagccatgtg gcagtagcgg
    35461 cagccgatac aaacatcttc atcaaccacc acaaagccat cttcacgctt atgcattgcg
    35521 ccgctggggc agacttttgt acatgcgggg tcatcgcaat ggttacagga gatggagaga
    35581 taataagcga acacgttctg gtgccagatg ccattatctt cctgccagtc gccgccagca
    35641 tattcataaa tacggcggaa actgacttcc gggccaagat ctttaaaatc tttgcacgcc
    35701 agttcgcagg ttttacagcc agtacagcgg ctggaatcga taaaaaatcc atattgtgtg
    35761 gtcatgggct actccttaaa ccttttcgat ctggacaaga ttgctgtgcg acgggtttcc
    35821 ctttgccagc ggtgaagggc ggtgagaggt cagaatattg atactgccgc cgtgatcgac
    35881 ccggtcacca aacatatccg ctttaagcca cgcaccttgc ccgatggcgg taacgccagg
    35941 cagaatacgc ggagtcactt ttgcggcaat cagcatttct ccattattgt taaatacccg
    36001 cacggtatcg ccatgacgga taccgcgtgc ctgagcatca atggggttga tccacacctc
    36061 ttgtgggcag gcctgctgta acacatcaat attgccgtag ctggagtggg tacgcgcttt
    36121 ggtttggcga cttcactcag catccagtag atgggtttgc gttcaaattt tgctgaggtt
    36181 gcgggttggg cgaggatcac atagcccata ttccctgcag attcatgaga gataaggtct
    36241 tcttgctctg ttggcatcag gtcaggcaac aggatatcgc aatacttagc cgaggccgtc
    36301 atgaagtggt caatgccaac aatcatctcg cacttgctgt catcctgaag cacctcatgg
    36361 gtgtgattga tgtcgccatg ttgattgatc aatgtgttac tggcgtagca ccataaaaac
    36421 ttgatgggga catccagttt ttcttttcca cgaacaccat cacgggtcgc ggtcattttc
    36481 gtaccatggt cgatggcatc tgtccatgta aaaacggaaa tctgcgtttt aacaggattc
    36541 tcgagcatcg ggaaccattc tacccccaga tcccagctac cttcgcgtac acctgagttg
    36601 ccgccgttta tgccgacgtt accggtaaga acggaaagca tggcaatagc gcgggacgtt
    36661 tgttcgccgt tggagtgtcg ttgtggcccc caaccctgac aaatataagc aggttttgct
    36721 gaaccgatct ctcgtgccaa ctggataatt ttttctgccg ggatgctggt gatttttgct
    36781 gcccattccg gcgttttagc tatgccgtca ggcccttcgc ccagaatata ggctttataa
    36841 tgcgcgttac gtggtgcgtt ggcgggcagc gttttttcat cgtaaccaac acaatatttg
    36901 tcgagaaatg gctgatcgac catgttttca gtaatcagta cccaggcaat cgcacaggcc
    36961 agtgcgccat cggtgccagg gcgaatgggc agccattcat cttcacgccc ggcagctgtg
    37021 tcgttatagc gtggatcgat gacgatcatg cgtgcgtttg aacgttcgcg ggcttgctcg
    37081 acgtagtaag tgacaccacc gccgctcatc cgcgtttctg ccgggtgatt tccgaacata
    37141 acgaccagtt tcgtattggc gatatcatcc gggctgttgc catcattggc accgaacata
    37201 taactcattg cggcactgat ctgtgcggta ctgtagctgc catagcgact gagaaaaccc
    37261 ccgcaagagt tcatcagacg gtacgggacg ttcgagttgg tgatatttcc gccatctacg
    37321 cctgttccgt acaggacatg tacagcctca ttgccgtaat ctttcaggat ccgccgaaga
    37381 ttatcactga tggtatccag ggcttcgtcc caacttatcc gttcaaattt accttcaccg
    37441 cgctcgccga cgcgcttcat gggatatttc aacctgtcag gatgattcat ccgtcggcgg
    37501 atagagcgcc cgcgtaaaca tgcccgaacc tgatgattac cgtaaacgtc gtcacctgtc
    37561 gtatcagact ccacccagta cacggtgtca tctttcacat gcaaacgtag cagacagcgg
    37621 ctcccgcagt taacggtgca ggaactccag accgttttct cttctaccgg agcctctgcc
    37681 gcccggacca tttgggaaaa tggcagagtg aaagcactgc ttgccagcgc gagactgcca
    37741 agtgcggagg ttttcatcag acttctatgg ctgatttcag ccttcatgag cgcctctgtg
    37801 gtatggattt tcatcattac tcacttattg cttttcaaac aaaatgtcat gccagaattt
    37861 atggttgtag tgggttatat tttttcgatc tcgaccagat tagtgtgctg cgggtttccc
    37921 ttcgccagtg gtgaagggcg cagagtggtt agcgtattca cacatccgcc atggtcgatt
    37981 ttatcgccag acatattggg ctcgtgccag gctccctggc ccatagcgct aactccaggg
    38041 agaatgcgtg gtgttacttt ggctggtagc cgaacttcgc cacgatggtt aaacacccgc
    38101 accatatcgc cgttggcaat cccacgtttc tgtgcatcta tagggttgat ccacacctcc
    38161 tgacggcagg ctgccttcag gacatcaatg ttgccgtagg tcgagtgagt acgggatttg
    38221 taatggaaac caaacagttg cagtgggaag gttctacgtt caggggagtc ccagccttca
    38281 aaggttgagg cataaactgg cagtgggctt atcacttcat ctttttccag ttcccaggta
    38341 cgggcaattt ccgccagctt gctggaataa atttcaatct taccggaagg cgttttaagt
    38401 ggatttgcct cggggtcgtc acgaaatgct ttgtaggcga caaaatggcc attgggatct
    38461 ttacgcttat agatacccat ttttttcagt tcgtcgtaag acggtaacgc cggatctttg
    38521 gcaagcattt tggcgtacag atgttgtaac cattgttcct gcgtgcgacc ttctgtgaac
    38581 ttttgataga cgtcaggtcc aagacgtttc gcgacttcac tcaggatcca gtaaatcggt
    38641 ttacgttcga atttttcgct ggtgactggc tggaggaaaa tgagatatcc catgttaccg
    38701 gcgtagtcgt taggaataat atcttcctgc tcaacggtca tcaggtctgc cagcagaatg
    38761 tcggcatatt ttgccgatga ggtcataaag ttttcgatga ccacaatcat ttcgcatttc
    38821 gattcgtcct gcagaatttc atgcgttttg ttgatgtcag aatgctgatt aacgagggta
    38881 tttcccgcgt agttccagat ggactctact gtcagacgga tggacggact tgaagtagtt
    38941 gactcaacgc tacttaaggt cctgatatga ctgacattaa ccatgacgat actgcatatc
    39001 catccagttc tttctctggg tctccctatc tgtatcagca agtctacacc tcaagtcctg
    39061 acacaaggct gacacaaatc aaggaccttg ctacgccaca gctagtccgg agaaccaacg
    39121 ggcgttacac tgtccgcttc agacttaaag gagctaccag cccgtttcta tcagtctcaa
    39181 cccgcagcac agaccggagg accgctacaa tgcgtcagag agagctcgca gccacagcta
    39241 aggcattcct actggacaat cctgaagtca cactacagga gcttacagcg cacatacggt
    39301 ccatggctga gcagttcctg actgatgcca gtgatgacta ctggaatggc gtagaggtgg
    39361 cgacactggt tgacgagaag tccaacctca gggaacttgc agcgacaaga gcgttgagtg
    39421 tggaccagca gaggggcatt aagttagcgc tggaagtcct gaccgcagca cagcagagag
    39481 tcgacactgg tgatacctct ggtctgatta agctgattga tggtggcgta agccagctta
    39541 ctgatgataa taaccatact gattactcaa ctataggcga ctctacgtcg attctgaaga
    39601 atgagcaggg ggatagacca gcagttttca cacaggaacg tcagttactc actccagagt
    39661 cacgagaatc atctgtactt tttcaggaat tggtgacatc actgctggct gagaaggtcc
    39721 agacactgaa acaatccagc tttaaggact tgtcagcgtc c
//
LOCUS       DAJVRP010000028        38775 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVRP010000028 DAJVRP010000000
VERSION     DAJVRP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.028"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OQN12_001491"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OQN12_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS1653873.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="OQN12_001492"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="OQN12_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1653874.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="OQN12_001493"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="OQN12_001493"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1653875.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="OQN12_001494"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="OQN12_001494"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1653876.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="OQN12_001495"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="OQN12_001495"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1653877.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="OQN12_001496"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="OQN12_001496"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1653878.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="OQN12_001497"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="OQN12_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1653879.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="OQN12_001498"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="OQN12_001498"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1653880.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="OQN12_001499"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="OQN12_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1653881.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="OQN12_001500"
     CDS             7138..7749
                     /locus_tag="OQN12_001500"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1653882.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="OQN12_001501"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="OQN12_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1653883.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="OQN12_001502"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="OQN12_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="OQN12_001503"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="OQN12_001503"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1653884.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="OQN12_001504"
     CDS             complement(11943..12302)
                     /locus_tag="OQN12_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653885.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="OQN12_001505"
     CDS             complement(12299..12805)
                     /locus_tag="OQN12_001505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653886.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="OQN12_001506"
     CDS             complement(12951..13514)
                     /locus_tag="OQN12_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653887.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="OQN12_001507"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="OQN12_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="OQN12_001508"
     CDS             complement(14722..15096)
                     /locus_tag="OQN12_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCS1653888.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OQN12_001509"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="OQN12_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCS1653889.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OQN12_001510"
     CDS             complement(16268..17173)
                     /gene="rfbG"
                     /locus_tag="OQN12_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCS1653890.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17166..18056)
                     /locus_tag="OQN12_001511"
     CDS             complement(17166..18056)
                     /locus_tag="OQN12_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCS1653891.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="OQN12_001512"
     CDS             complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="OQN12_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCS1653892.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="OQN12_001513"
     CDS             complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="OQN12_001513"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCS1653893.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="OQN12_001514"
     CDS             complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="OQN12_001514"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1653894.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="OQN12_001515"
     CDS             complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="OQN12_001515"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCS1653895.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="OQN12_001516"
     CDS             complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="OQN12_001516"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1653896.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23202..24095)
                     /gene="galF"
                     /locus_tag="OQN12_001517"
     CDS             complement(23202..24095)
                     /gene="galF"
                     /locus_tag="OQN12_001517"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCS1653897.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="OQN12_001518"
     CDS             complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="OQN12_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCS1653898.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="OQN12_001519"
     CDS             complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="OQN12_001519"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCS1653899.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="OQN12_001520"
     CDS             complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="OQN12_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCS1653900.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="OQN12_001521"
     CDS             complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="OQN12_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCS1653901.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="OQN12_001522"
     CDS             complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="OQN12_001522"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCS1653902.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="OQN12_001523"
                     /gene_synonym="manB"
     CDS             complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="OQN12_001523"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1653903.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="OQN12_001524"
     CDS             complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="OQN12_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1653904.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="OQN12_001525"
     CDS             complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="OQN12_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS1653905.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="OQN12_001526"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35503..35982)
                     /gene="gmm"
                     /locus_tag="OQN12_001526"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1653906.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="OQN12_001527"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="OQN12_001527"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="OQN12_001528"
     CDS             complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="OQN12_001528"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1653907.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="OQN12_001529"
     CDS             complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="OQN12_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1653908.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38654..>38775)
                     /locus_tag="OQN12_001530"
     CDS             complement(38654..>38775)
                     /locus_tag="OQN12_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS1653909.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10358 a         9708 c         8722 g         9987 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaacccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttta tcagcctttt
    16321 tccgccttaa cttccaaaaa cttaaagcca agatcaaatt gcctatataa cctttaccca
    16381 attgattatt cacaaagcta tcatcagcta aggcattagc tattttagtt acttcttttc
    16441 gataccactt gtttctcacc aaaccaagcc gtttgtattt tgctttaaaa ctaatgtttg
    16501 ctcccacttg gttgttttca tgctgtctgt acagcattgt tggttttcta tcaatatacc
    16561 acgaataatt tctcgtccgc gcaaatgaat ataaaaacca atcatgcaga cagatatctt
    16621 gtgcagcatt tttattattt atgatgaact tcttaaattc aataagcgtt tcttttttta
    16681 taacatatgt acaccccgga cctgctgctt caaaaaaata atcgaattga gtttgcgact
    16741 gagccttatc aactaaattt tttcgccctg atggataata tgcaattaca ttgctggaat
    16801 aacaattcgc attattaaat accaagcagt caattgcatt ttttatttta tctttatacc
    16861 aaatatcgtc ctgatcacta aaacagatat aatcatagtt ttcaagatcg acatctttaa
    16921 ttaatctata aaaattttta cctgcccccc caaacttatt gacgcttgga agtatattta
    16981 taattgaggg atatgataat tgaaattctt cacaaatatt taatgtatta tcagttgata
    17041 aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata ctttttaatt
    17101 gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact cttttttgat
    17161 cagaactatg cattataacg accgccccca gtaattcctc ttattccatc cagcgtagcc
    17221 aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat taaagtcgta
    17281 tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg attttttcta
    17341 agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg aattggtatg
    17401 ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc tatatcagga
    17461 atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt atcgacataa
    17521 tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt tgaaaccatg
    17581 gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt gacatgaatt
    17641 ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga cttatctcta
    17701 tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca ggaaagtttt
    17761 ttgataaact cattgcgaat acggctatct tgatcaaaaa aaattatctt ctctgcccct
    17821 ttttcaagag aatatcgaat cccaatattt tgtgcttttg ctataccttc attcctacca
    17881 agacaaatta aatgaacttt ttcaatgtct gctaatttat ctttcaaggt gaaattagtc
    17941 ctattatcga ctactacaac gtaacaattt tgctccttta gttcagtgat cagtgcattc
    18001 agatttttaa gttcgggatt atatgtcaca atgacagcgt aaatattact attcattatc
    18061 caagtgactc agtaattggt taattttagt tttcatatac agaacacatt ctagtataaa
    18121 gccattgtaa taacagagca tctaaaatta cacgtatgct ccaggatatt gcggcaccca
    18181 gtaacccata tttttcgacc ataaaataca aaagggctaa ataaggaacc aattcagcac
    18241 aatgaatcaa agcagtaatt ttagattttc ctaatgcctg gattgcagag aatggtatca
    18301 ttgccagcgc attgaagaaa aaaccgatca ataacacatt taatatatta aaggaattaa
    18361 ttttataatt ctcaccaaac catattttca atacaagccc tgaatataac aagcctatga
    18421 ttatcaccgg caaacagatt agaaacatta aaagcaatga tttatttaca ttacgtttaa
    18481 attctttaaa gtctttgatg ttacttaacc ttggaaacac tgccctcccg attgctgcag
    18541 gtattattcc taattttaga ataacctccg ctggtgcaga ataaaatgca actttatctg
    18601 cccccataat atttgacact ataaatcgat caaaatacac catgaccgga cttatgatat
    18661 tacttactgt catccaaccg ccaaagaaaa agagtcgttt aaaggtttta taacaaaaat
    18721 gaacccccga aattttaata tcattcctga cataataggc agagactaaa atcgaaataa
    18781 ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt agcactataa aaaacaaata
    18841 tggccggaat tcccgcgatg caagagcttg atatagattt ttgaatattt acaatgccaa
    18901 atttttcatc cccctcaaga atggctgacc ataattgatt tagaataaat aatggaatgc
    18961 aaatagccaa cagtttaaat gctagttgta tatcactatg ttcaacacca gaaattttta
    19021 gataattaac aattccatca gagaaaatca ataataaaaa agcaccaaag catgaaaata
    19081 gcaccaaaaa agatgtactt gttgaaatta ccttggttct ttcatgatga ttatcacgat
    19141 gaattgcaat ttctctaata acagagcgag tcaggcctac atcaaaaatt ccagcatacc
    19201 caacaagtgc aattgcaatt gtataaatac cgaaaccttc aggccccaaa cctcgagcga
    19261 gaaatcctag cgaggggata gcaattaaag ttggtattgc atagccaaaa aggttccaga
    19321 cactattttt tattatgctc attattacat ttctttatca tataaaataa tcagcatcta
    19381 caaatttttg accattaata tctttatcag aaagcaatgg attttgaacc ggccattcta
    19441 tatttatact tttgtcgctc cataaaatcc cacgctcttg catttttgaa tagaaattat
    19501 ttgttttgta gacaaattct gcttcatcac taagaacgca aaacccatga gcgaatcctt
    19561 ccggtatcca taattgacgc ttattctccg ccgacaaatt tacaccaacc cacttgccaa
    19621 atgttggtga tgatttccga atatcaacag ctacgtcaaa aacttcaccg acaacacagc
    19681 gtaccagttt tccttgagca taaggttcca gctgatagtg cagtccgcgt aacacaccct
    19741 tagttgattt agaatggtta tcctgaacaa actcaacctt tcgtcctaca gcctcttcga
    19801 aaactttctg attgaagctt tccataaaaa agccccgctc atcaccaaat acttttggtt
    19861 caaaaattag cacatcagga atttcagttt taattacgtt cattttatta ataaccttta
    19921 atcattttca gcagatactg accataagca tttttcttca gcggctcggc taataccttc
    19981 acttgctcag catcaataaa gcctttacgg taagcaattt cttccgggca ggaaactttc
    20041 agcccctgac gctcttcaat tgttgcaatg aagttgcttg cttcaataag actttgatgt
    20101 gtccccgtgt ccagccatgc ataaccacgc cccatcatag cgacagacaa acgtccttgg
    20161 tccatataaa tacggttaat atcggtaatt tccagttcgc cacgtgcaga tggtttaagg
    20221 tttttcgcca tttctacaac gtcattgtca tagaaataaa gcccagtaac cgcatagtta
    20281 ctttttggtt ccagcggttt ttcttccaga ctaattgccg taccgttatc atcaaattcc
    20341 accacaccgt agcgctcagg atcatttacg tgataggcaa aaaccgttgc gccactctct
    20401 ctgttaacag cggtatccat taatttcggc aggtcgtgac catagaagat attatcacca
    20461 agtaccagcg cacaatcatc accacctata aattcttcac caatgataaa tgcttgcgcc
    20521 agaccatccg gactcggttg tactttatat tgcagattaa gcccccactg gcttccatca
    20581 ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac tgataatcag aatatctcga
    20641 atacccgcca acataagcgt tgaaagcgga taataaatca tcggtttatc ataaatcggt
    20701 agcagttgtt tgctcacagc cattgtcaca ggataaagac gagtgccgga accaccagcc
    20761 agaataatac ctttacgcgt tttcatttca ccattccttt taattcatct cgctctagca
    20821 tcatgagcga gatgcaataa tttgttaaat tgccgtggtc gtaaataatt cgttgagcat
    20881 acgtttcacg cccacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc
    20941 ggtattgagg cgagaattat ggggacgacg tgctggtgtg ggataggccg ttgtcggcac
    21001 ggcattaagt ttgttaagag caaggttgat ccccgctctg cgcgcttctt caaataccag
    21061 agcggcataa tcgtgccagg ttgttgtacc accagcaacc agatggtaca aaccagcaac
    21121 ttctggttta tttgcggcca cgcgaatggc atgagcggtg caatcagcca gtaattcagc
    21181 ccccgttggt gcgccgaatt gatcgtttat tacagccaac tcttcgcgtt cttttgccag
    21241 gcgcaacatc gttttggcaa agttatttcc tttaccagcg tatacccagc tggtacggaa
    21301 aataagatgc tttgcgcaat gttcttgtaa cgctttttcc ccagctagct tggtttcacc
    21361 gtagacattt agcggtgctg ttgcatccgt ttccagccat ggcgtgtcac cgttgcctgg
    21421 gaaaacataa tcagtggagt agtgtataac ccaggctccg acttcattag ctgcttttgc
    21481 aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa ttcggttctg attccgcttt
    21541 gtctactgca gtgtgagcag cagcattaac aataacatcg gggcgaattt ttttgacggt
    21601 ttcagccaca ccttcggggt tgctgaaatc tccacaataa tcagtggagt gaacatcgag
    21661 cgcaatcaag ttacccagag gagccagagc acgctgcagt tcccaaccca cctgccctgt
    21721 tttgccgaaa agcaggatat tcattactga cggccctcat agttttgttc aatccacgac
    21781 tgataagcac cgcttttcac attctcaacc caatttgtat tagccagata ccattccacc
    21841 gttttacgaa tcccgctctc aaacgtttct tgcggtttcc agcccaattc gcggctaatt
    21901 ttatcggcat caatggcgta acggcggtca tgccccgggc gatcggcaac ataagtaatt
    21961 tgctcacgat aagatttctc tttcggtaca atctcatcca acaaatcaca aatagtgagc
    22021 actacgtcga tgtttttctt ttcgttatgt ccaccaatgt tataagtttc acccgcttta
    22081 ccttcggtta cgacggtata taacgcacga gcatgatcct ctacatacaa ccagtcgcga
    22141 atttgattcc ctttaccata aataggtaat gccttacctt ccagagcatt cagaataacc
    22201 aatggaataa gtttttccgg gaagtgataa ggaccataat tgttcgagca attagtcaca
    22261 atggttggta aaccataagt ccgcttccag gcacgtacca aatgatcgct ggaagcttta
    22321 gacgcagaat acgggctact tggcgcgtaa gctgtcgttt ccgtaaatag cggtaacgct
    22381 tcgttattat ttgcttcatc cggatggggt aagtcaccat aaacttcatc agtagaaata
    22441 tgatgaaagc ggaaactttt tttcttttca tcattcagag cagaccagta attgcgcgcc
    22501 gcttctaaaa gaacataagt ccccacaata ttggtttcaa taaatgccgc agggcccgtt
    22561 attgaacggt caacgtggct ctctgccgcc aagtgcatca ccgcgtctgg ctggtgctgt
    22621 gcgaaaatac gcgacatggc tacagcatcg cagatatctg catgctcaaa agcataacgt
    22681 tcagaatcag aaacatcagc gagtgattcc aggttcccgg cgtatgttaa tttatcgaca
    22741 ttaacaacac tatcttgcgt attatttatt atgtgacgaa caacagcaga accaataaac
    22801 cctgcgccac cagtaacaag tatcttcact tttttattcc atatagctat agagcatgct
    22861 gtgaaataga ctgctccaga tttgattaat agaggtatta atacacgcta ccgcccctgg
    22921 cttaacagct accagtgcac tgcatgcatc tcaacgatgt ggcgagcgtt agccactcgc
    22981 gccaaatccg aaaaattcaa acgttaattg tcttaccaaa cagccgtaga aacaaggaaa
    23041 atcctggaaa aatttgaata atatgagcta tgtaacttct tgtttttata ggctttataa
    23101 aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg ccacgaatct tcactgccgt
    23161 tataattttc ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc
    23221 tcaatacctt tacggaactt cgccccttct ttcaggttgc gtaggccata cttcacaaac
    23281 gcctgcatat agcccatttt tttgccgcag tcgtaactgt cgccggtcat cagcattgca
    23341 tcaacggatt gttttttcgc cagctcggca atagcatcag tcagctgaat acgtccccat
    23401 gcaccaggct gagtacgttc cagttccggc caaatatcgg cagaaagcac atagcgacct
    23461 acggccatga tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca
    23521 atgcggctga ctttaccctc acggtccagc ggctctttag tctggatgac ggagtattca
    23581 gagaggtcac ccggcatacg ttttgccagc acctggctgc ggcccgtttc gttgaaacgt
    23641 gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc tggcatcgtc gatcacaacg
    23701 tctggcagta ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca caaaatggag
    23761 tggcctaaac ctaaaggttc gccctgacgc acgttcataa tgatcacgcc cggcggacag
    23821 atggactgta cttccgccag cagttgacgc ttcacgcgct gctcaaggag tgattctaac
    23881 tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt taccaggagg
    23941 atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg
    24001 acgattggta gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccgaga
    24061 cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg taaaatggtt
    24121 gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa ttgattactg
    24181 aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt cctaagtatg
    24241 gctccatttt tccaggaatg gtcgcaaatc tactccctcc gttccggcaa tctaaagtta
    24301 atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga gctttgccct
    24361 ctttcattca ccgcatgaac atttgcaaga gacagcagtg tttctttttt cgccataaag
    24421 acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    24481 tcctgcatca cattgatatt tcgcataaaa agatgttgcg gcttgttgtg taactccagc
    24541 gaggcacgct tcatctcaat gttagttagc gacacaaagg agacggcatt cccggcggag
    24601 atttggatgc cgcgcaattt ataagcaaga tgggtattat ccagttgaat attattcact
    24661 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    24721 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg
    24781 actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt gcgggcattg
    24841 atattacgaa taacaaaatg tttaccattt tcaacatgga tcaactgccg acaatccgat
    24901 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttgttatca
    24961 taagtgcttc ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt ggtacagttg
    25021 atacgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc cacgttccat
    25081 tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg taatgttggc accgataatc
    25141 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    25201 atggtcaggt tgcgcatgac ccgtttgttt ttgccgccaa tataaatctg cattaccggg
    25261 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aacaacgcac atccagggta
    25321 atgttatgca taccgccgcc ctctccccct gtcacctggc tgccgtcctg taagacaaat
    25381 cgccctctgc cgttgccgcg caggcttcca aggatgtgta acgttttgcc gggagggatg
    25441 aagatgccgg tgttgatatt gtcacaaacg aatccggcag gcacgacgac cgtttgccct
    25501 tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg ggttgtagtc gttaatgtta
    25561 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    25621 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatacgttc tcctctataa
    25681 agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat cgtgttcgac
    25741 tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg gtatccagtt ggctaaacgc
    25801 cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc agcagccagc cggatttatc
    25861 ggcctccacc agttcaggaa ttccgctatg cagagtagaa accaccggaa taccgaccgc
    25921 catcgcttcc attagcgcca ccggaatgcc ttccatatcg ccatccgcgc ccgttaccga
    25981 tggcaacagg aagacatccg cgtcgtcgag catctccttc acttcatggc tcggtttaaa
    26041 gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtgc gcaggcgtcg
    26101 ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    26161 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacagatat
    26221 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    26281 gcccatacgc gatacggcga ttttttctct ggggcagccc attttttgca gccttccggc
    26341 ccacagatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    26401 agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga tggtggcaat
    26461 tttgccgcga atgataccca gttcgcgtag ttttgctgcg gttacccccg ctgggccaaa
    26521 atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tgaccacaaa tggcagacaa
    26581 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcatgcc aggtattttt
    26641 acgatgaatg ccacgtaagg tctggctggc gcggtggcgc agtttcgcta ctttgcccgc
    26701 aggttcgtcc tgtaaccagc gggttctggc gacaaggttg tatttcgtcc atgccgcgtg
    26761 ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc acttcaaagc ccatatcaat
    26821 aaacgcggta atttgattga ggacgaaggt ttccgacgac agcgggaatt tcaataagaa
    26881 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    26941 cccgtctgac gttcgcgact gacggcttcg ttaaatcgag cgttcagcac cggaagctgg
    27001 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg aatatcaatc
    27061 gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc atagttgatg
    27121 gcaatcgccg gagtagcaaa gttcattgag ataatggcgg agtgcaggcg cgtaccgacg
    27181 gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc atccatcact
    27241 acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg
    27301 tctttgttat agctgtcgat acccgtacag gtggagagcg caatcacctg atagccttca
    27361 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    27421 agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ccactgtttt ctgttgtgcg
    27481 gcaacgtcca gccagtgttg aacggcatag ctggcggtga agtcttcggt gtggtgatcg
    27541 accagccacg cggtatcgac gccatgttcc acttttgcgg tggtgatatt tctgcgtttc
    27601 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaagacatag
    27661 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    27721 agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    27781 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag caggcgcacg
    27841 aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc ggtgacgcgt
    27901 gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaatgcg cccgacaacg
    27961 cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg gatcgcccat taccgggcgg
    28021 ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggtatgtgga
    28081 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    28141 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg
    28201 aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg cgcgtcttat
    28261 caggcctaca ggtcccgagc acagaaccgt aggacggata aggcgtttta cgccgcatcc
    28321 ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc ccgtaaaagc attttcattt
    28381 tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgt gacagcacaa
    28441 tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc atccccagcg
    28501 ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc ggcaacgaga
    28561 gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaacca agaaccggtt
    28621 taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg aaaccgagcg
    28681 tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata aacagaaatg
    28741 ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc agcagcgaac
    28801 caatcgggtt acccacggag cgcagcagac ccacaataca cagcaattgc agcaccggaa
    28861 taatgctgtt ccacttttcg ccgaatacca gcggtacgaa gttattcgac accaccatca
    28921 gccccagcag cgccggaaag ttgataatcc ccactatcga cagcagcttg tagaagttaa
    28981 cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc cggaaacaac acgcgggtga
    29041 tgattgggtt cagcttcatc ggcggtacga cggccacgtt gtacgccagg ttgtatcccc
    29101 ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt tgaaaggttg gtgttgagat
    29161 agttgatgat gctgtccgcc gtcagccagg caccaaaacg taagttcggt gccacagacg
    29221 ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc aaagtagcca aacagcagtg
    29281 ttctcaccgc actattgacc agataaccga ggatcgcggt catcgccagc ggccagaaat
    29341 gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg atcatgccga
    29401 ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg tgggggatca
    29461 ccacaaacgc cagcgacaat gttttaatca acggtgccaa gtccgggtta ttcagcacgt
    29521 cgccgatgac atcactcaac aaaaacaccg ccacacatac cacaagcccc agcccgacgt
    29581 tcagccagta cagcgtggtg agttcaaggt gactgatttc ttttcgctga ataatcgagt
    29641 tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat aatcaccagc gacacagtca
    29701 gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc accagcccga
    29761 ggccgatgat gatcaccgtg gcaatagccg accacttcgc gccgctgatg gttttttcac
    29821 gtaagctcat atcaatatgc cgctttgtta acgaagccct tgaataccgt caggaaaacg
    29881 attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa ctcgacgcgt
    29941 ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg cgcccagccg
    30001 gtaatgcccg gtttcacttt atggcgcagc atgtagcctt caatgagctg gcgatactgt
    30061 tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg acatcccccc ggtcagcaca
    30121 ttgataaact gcggcaattc gtccagcgag gtgcggcgca gaaagttccc cactttagtg
    30181 acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat cactttcatg
    30241 gaacggaact tccacacttt gatcggcttg ccatccatac catagcgagt ctggcggaaa
    30301 ataaccggcc ctggtgaact gagtttcacc gccagcgcaa tacagcacag caccggggag
    30361 atcagcagca gaataagcgt cgccagcaca atgtcttccg cacgttttag caggcggtta
    30421 acccccgaaa gcggcgtgtc atacagcggc accaccggaa cgccgttcat ctcttcgaga
    30481 cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca gcagcaccga acaggtggta
    30541 tccgccagtt gatggaccag ttttttcact cgtgcgccgt cgcacatttg catcgcgata
    30601 tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag gttacccgcc
    30661 cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac ttcaaaccct
    30721 aaccacggct gattacggaa actctccatc agcatttgtc cggcggctaa atcccccact
    30781 acggcgacca tccgcttgtt gtagccatga ttacgcagcc agcccgcccc aatgcgaata
    30841 catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag ccagattttc
    30901 agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat cacgcttaag
    30961 gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg ccatgagcga
    31021 taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag cgccaccaac
    31081 aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag cccggcaaac
    31141 atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt ggttttcgct
    31201 cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt acccgcagtg
    31261 gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg cgttcgcgct
    31321 tccatcagcg gcacatcacc gcgcgattcc acattcaggc gcaccaccgg ttcggtattg
    31381 gaggtgcgca ggttaaagca ccagtcggca aaggtcatgc tgatgccatc gctgcgatcc
    31441 accgccagcg cctcacggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc
    31501 gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc gcgcaccagt
    31561 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    31621 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc gccaccgtag
    31681 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacattacc
    31741 ggcgtgccgc ctgcagcagt caccacatca atagtgttcc aagagagacg cggatcatgg
    31801 atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc gacaatgtag
    31861 tagccctcga taaactgccc tttttcgtca aacagaaaac agcggtcaaa atcgccatca
    31921 aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat tgcgggtgtc gtcgcgacat
    31981 tccggcagca gcgggttagg aataccgttg gggaaattgc cgtccggtgt gttgtgtact
    32041 ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc gtccaccacc
    32101 ggacccgctg cgccattccc ggagttgatc accagcttga gcggcgtgag atttttgaca
    32161 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg ctgatagcga
    32221 ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccagacg ctggacgtcg
    32281 cgcagtccgg tatcaccgct gatcgtgcga gccccctcgc gcaccagctt catgccgttg
    32341 taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc gagatggaac
    32401 gtggcgaaat agatctcttc ggtgccggac ataccgatat ccagcacatc gacgcccgca
    32461 tcctgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgagacg gacatcaccg
    32521 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    32581 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    32641 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    32701 tttatttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc gacgctgccg
    32761 cgtcttatca ggcctacaaa accgaaccgt aggtcggata aggcgttcac gccgcatccg
    32821 acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa gcgcaccaca
    32881 tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg aattttcccc
    32941 gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tggactcgtt ttcaccaagc
    33001 agtttgatat caccatcaat ggtgactttt gccgttcccg cgaccactac ccagtgttcc
    33061 gcgcggtgat ggtgcatctg taccgacaaa ccctcgcccg gtttcacggt gatgcgtttc
    33121 acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacggacg atacacttcg
    33181 cgatgcaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac ttttttcaca
    33241 tcctgtaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac cactaccaga
    33301 tctttcaccc cgacggtggt gaccaggcct gattcggcat acacatagct gttttcagtt
    33361 ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc gctgatctcc
    33421 cataatgaag accaggaacc aacatcgctc cagcccgcat ccatcggcac cacaacagca
    33481 tctgccgtag gttccatgac cgcgtaatcc accgactctt ccgggcaggc gagaaacgct
    33541 tcttcatcca cacgaataaa atcgagatcc ggatcgacgg cgctcatcgc tttttcgcag
    33601 gcatcgagga tatccgggcg atattttttc agttcttcga gatagcgtcc ggcgcggaac
    33661 aggaacatgc cgctgttcca gtaatattcg ccgcttgcta cataggcctg ggcggtttcc
    33721 agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg ctcacccaca
    33781 gacacttcac cgcgacgaat atagccataa ccggtttctg gtagatccgg cacaatgcca
    33841 aaggtcacca gcttgcccgc ttcggcataa ggcatggcat tacgcacggc ggcacggaac
    33901 gcgtcttcat cggcaatcac atgatccgcc gccaatacca gcattaacgg gtcgctctcc
    33961 gggctatgac gttttgccgc cagcgccgcc agtgcaatgg caggtgcagt gttacgccct
    34021 gccggttcga gaataatgtt ctcggtgagt ttgttcagtt gacgcagctg ttccgcgaca
    34081 ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac gccgttcagg
    34141 cggcagatgg tggtttgcag catggtgaga tcgcctttca ggcataaaaa ctgcttgggg
    34201 taaagtacgc gggaaagcgg ccataaacgg ctaccggagc cacctgccat cacaactgga
    34261 tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga cgtaacacgt
    34321 tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttatgt ttgggcagca
    34381 ggagcgcctg acgaatcccc gccaccagcg cctcgaccga ttccggttct acgcaaaccg
    34441 caatacccgg aaaggtttcg caaagctgcc ccagttctgt gtgggcctct gcggtaatca
    34501 ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg gcatctgccg
    34561 cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgca ggtaaagcgt
    34621 catacgattg cagcgggaaa aattgcatgt tgcgcagtcc acgctgctgc gccatttttt
    34681 ccagccgcgc tttgccgccg ctctgcccga caatggcaaa aatcagcggt tcatcgcgca
    34741 ggagatcggc agcttcaata acgttttcca gcccctgctt ttcaccaata ttgccggagt
    34801 aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca tcaacatcgg
    34861 cgtctgcaac atgctgaaaa cgggcaattt ccgaccagtt ggggaagaag atgacgttat
    34921 ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc gtggaaacgt
    34981 tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc actttgccgc
    35041 ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa tcctgaatat
    35101 gcagcacggt acgcgcacca gagagcttcg ccagcaggcg cattcccggc gtgcaaaaca
    35161 gcgttggcac tacgccaata atgcgatccg gcttccagcg acgttgcgcc atcagtggaa
    35221 agaaactgct gacggcaaaa ctgccgagat gcaacaagcg tttcagggtg ctcggctgtt
    35281 ttggcacgta cagcgggcag cgccacaccg tggcagcccc ttcttctcgt ttgtagcgcc
    35341 aggcggaata gttctcaccc acctgccatt gcgggtagta aggcggtgcg gtaatgaccc
    35401 gcacctcatg accttgtgcc gccaaccatt ccaccatctc gccggtgtat ttgccgatgc
    35461 cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc cgggtactcc
    35521 ggcacgcttc tcagcgagaa aataggctcg gctgttagcg tgaacatcgt tactggcgag
    35581 cagcgcgtcc ggcgtcagcc agcggtaatc gtcatgctgc tcatccggca gtaacagctc
    35641 ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga aatccgtgcc
    35701 ggagaagtta tcgtcataaa aatgctgcca gacaccgtaa aactggcctg ctgttatcgg
    35761 cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca gcgtttcgtc
    35821 tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt tggttctttt
    35881 gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga caagcggtgt
    35941 ggagcgcact acagtggcaa agtcttcctg acgtaaaaac atcattcccc ccgaaagcgg
    36001 tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag tgagatttcg
    36061 tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg cgtaccatcc
    36121 ggtttgctgg catcaaaaac cacccgacct ttgtaaccca ccactttggc gatggtttgc
    36181 gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga cagcatcggt
    36241 tgggtattct ccagccagac ttcatgcgcc agctccatga catgaatgct cgccgccgcc
    36301 atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccatac caccacgtcc
    36361 gtgccgtcgc ctcgtggaag cgacgcagca atgctggtat cacatgggag ttactcgggt
    36421 ggaagttgtc atgcggcccg tacaggttgg tcggcatgac agtgcggtaa tcgcgtccat
    36481 actggcggtt gtaagattcg cacagtttga tcccggcgat tttggcaatg gcgtaaggct
    36541 cgttggtcgg ctccagcgtg ccctgcaata actcgctttc tgccatcggc tgttttgcca
    36601 gtttcgggta gatgcaggac gatccgagaa acatcagttt gttcacgtcg ttctgatgcg
    36661 cggcgtgaat gatgttgctc tcaatcatca tgttctggta gatgaaatcc gccggatagg
    36721 tgttgttggc aacaatgccg cccactttcg ccgccgccag atagacctgg tcaatgcgtt
    36781 cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag gttcagttca tcgcgggtac
    36841 gtaataccag ctccacatca ccgcgctgtt cgagctgccg cttgatggca gaaccgacca
    36901 tcccacaatg gccagcgata aaaatgcgtt gtttactcat gcttatgact ccagcgcgat
    36961 cgccacgtcg tagccgtgag atttcagcag agagtgtttt ttcgccgctt cgaggtcatt
    37021 agccaccatt tcagacacca tctctctgag ggtgatttcc ggtttccagc ccagtttttc
    37081 gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttct gccggacgga agtaacgcgg
    37141 atcaacggcg ataatcacat cacccggttt aacgcccggc gcgtcatgcc cggtgacgga
    37201 aaccacaatg cccttctctt caacacccgt gccttcaaag cgcagtttga tgcccagctg
    37261 tgctgccgcc atttccacaa actgacgtac ggagtactga acgccggtcg cgataacgaa
    37321 atcttccggc tgttcctgtt gcagcatcat ccactgcatt tttacgtagt ctttggcgtg
    37381 gccccagtca cgcagggaat ccatattgcc gaggtacagg cacgactcca gcccctgggc
    37441 gatgttggcg attgcgcggg tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga
    37501 ttcatggttg aagagaattc cgttacaagc gtacatgccg taggattcgc ggtagttaac
    37561 ggtgatccag taggcgtaca gtttggcgac cgcatacgga gatcgcgggt agaacggcgt
    37621 ggtttctttc tgcggaattt cctgcaccag accatacagt tcagaggtgg aagcctgata
    37681 gaaacgagtt ttcttttcca gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc
    37741 catcgcgtcg acgtcagcgg tatattctgg tgactcaaaa gagaccgcaa cgtggctcat
    37801 tgcgcctagg ttgtacactt catccggctg tacttcacgc aaaatgcgcg tcaggttaga
    37861 ggtatcactc aggtcgccat aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg
    37921 ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac
    37981 ctcgtaacct ttttccagca gaaactctgc caggtaagaa ccgtcttgtc cggttacacc
    38041 ggtgatgaga gcgacttttg acatgtctta ttcctctgta tttttgaatt tattcagttt
    38101 caacgcgttc gcgtatcacc actgcgggat tcccccggca aaccacattt gccggaagcg
    38161 atttaaaaac actgcttcgt gcacccacga cggtgccgtc gccgattgtg acgcctgggg
    38221 caacaaagac atcggttgcc agccagcatt tctcgccaat cacaataggc gtggcgttaa
    38281 tggtgaaatg ttgacttgca tggtcgtggc taccggtgca taaataactt ttttgcgata
    38341 tcaccgaatg tgcgccaatg gttatttcac cgagggtata taaattgacg tcatcgccaa
    38401 cccacgcgta atcacctaag gttaatttcc acggataggt aatttttact gacggacgaa
    38461 taactacgtt ttttcctatt tttgctccga ataaacgtaa taaaaaagcc cgccagcgat
    38521 acaatacttg tggcgaccag gcaaatattg ttgcctgtac tgcccaccat aattgcactt
    38581 taatagcgtt gccgccccgg aaccctttcg gcaccgagaa accgcttaaa tcttgcattg
    38641 gttttcctta tattcagact ttgttatata aggctttagt cttacctgtc gtacgttggc
    38701 gtaaatgcca ggataattct gcccagaagc caggtacatg taatatctgc cgttggactt
    38761 ttttggcgtc agcac
//
LOCUS       DAJVRP010000029        37362 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVRP010000029 DAJVRP010000000
VERSION     DAJVRP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.029"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..176)
                     /locus_tag="OQN12_001531"
     CDS             complement(<1..176)
                     /locus_tag="OQN12_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS1653910.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OQN12_001532"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OQN12_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1653911.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OQN12_001533"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OQN12_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1653912.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OQN12_001534"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OQN12_001534"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1653913.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OQN12_001535"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OQN12_001535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1653914.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OQN12_001536"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OQN12_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1653915.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="OQN12_001537"
     CDS             complement(5180..5422)
                     /locus_tag="OQN12_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1653916.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OQN12_001538"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OQN12_001538"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1653917.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OQN12_001539"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OQN12_001539"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1653918.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="OQN12_001540"
     CDS             complement(8339..9313)
                     /locus_tag="OQN12_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1653919.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="OQN12_001541"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="OQN12_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1653920.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="OQN12_001542"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="OQN12_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1653921.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="OQN12_001543"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="OQN12_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1653922.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="OQN12_001544"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="OQN12_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1653923.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="OQN12_001545"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="OQN12_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1653924.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="OQN12_001546"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="OQN12_001546"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1653925.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="OQN12_001547"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="OQN12_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1653926.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="OQN12_001548"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="OQN12_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1653927.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OQN12_001549"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OQN12_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCS1653928.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="OQN12_001550"
     CDS             23180..23383
                     /locus_tag="OQN12_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1653929.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="OQN12_001551"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="OQN12_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS1653930.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="OQN12_001552"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="OQN12_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1653931.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="OQN12_001553"
     CDS             26388..26660
                     /locus_tag="OQN12_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1653932.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OQN12_001554"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OQN12_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1653933.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OQN12_001555"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OQN12_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1653934.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="OQN12_001556"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="OQN12_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1653935.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OQN12_001557"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OQN12_001557"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1653936.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OQN12_001558"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OQN12_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="OQN12_001559"
     CDS             complement(31907..32305)
                     /locus_tag="OQN12_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1653937.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="OQN12_001560"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="OQN12_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1653938.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33846..34274
                     /gene="rplM"
                     /locus_tag="OQN12_001561"
     CDS             33846..34274
                     /gene="rplM"
                     /locus_tag="OQN12_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1653939.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34290..34682
                     /gene="rpsI"
                     /locus_tag="OQN12_001562"
     CDS             34290..34682
                     /gene="rpsI"
                     /locus_tag="OQN12_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1653940.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35077..35715
                     /gene="sspA"
                     /locus_tag="OQN12_001563"
     CDS             35077..35715
                     /gene="sspA"
                     /locus_tag="OQN12_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1653941.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35721..36218
                     /gene="sspB"
                     /locus_tag="OQN12_001564"
     CDS             35721..36218
                     /gene="sspB"
                     /locus_tag="OQN12_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1653942.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36261..>37362)
                     /gene="dcuC"
                     /locus_tag="OQN12_001565"
     CDS             complement(36261..>37362)
                     /gene="dcuC"
                     /locus_tag="OQN12_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1653943.1"
                     /translation="MERIGASCAMVSLLSRPLKLIRSPYIILSATYVIGQIMAQFITS
                     ASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAAQVAGMKIAT
                     YFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYAILPV
                     MPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQAFFD
                     GMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMALVIAICA
                     IVMGFGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVVVVTSGI
                     AGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         9039 a         9342 c         9837 g         9144 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatacc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgtttg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gtctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 caggcaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacttggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag ggcaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctaggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacagcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttccccaaa
    33721 actttttgtg tgctggcata ggctattcga aggggtaggt ttgccggact ttgtcgtgtg
    33781 aacctcaaca attgaagacg tttgggtgtt caccaacgtg taactattta ttgggtaagc
    33841 ttttaatgaa aacttttaca gctaaaccag aaaccgtaaa acgcgactgg tatgttgttg
    33901 acgcgaccgg taaaactctg ggccgtctgg ctactgaact ggctcgtcgc ctgcgcggta
    33961 agcacaaagc ggaatatact ccgcacgtag ataccggtga ttacatcatc gttctgaacg
    34021 ctgacaaagt tgctgtaacc ggcaacaagc gtactgacaa agtgtactat caccacaccg
    34081 gccacatcgg tggtatcaaa caagcgacct ttgaagagat gattgctcgc cgtcctgagc
    34141 gtgtgattga aatcgcggtt aaaggcatgt tgccaaaagg cccgctgggt cgtgctatgt
    34201 tccgtaaact gaaagtttac gcgggtaacg agcacaacca cgcggcacag caaccgcaag
    34261 ttcttgacat ctaatcggga ttataggcaa tggctgaaaa tcaatactac ggcactggtc
    34321 gccgcaaaag ttccgcagct cgcgttttca tcaaaccggg caacggtaaa atcgtaatca
    34381 accaacgttc tctggaacag tacttcggtc gtgaaactgc ccgcatggta gttcgtcagc
    34441 cgctggaact ggtcgacatg gttgagaaac tggacctgta catcaccgtt aaaggtggtg
    34501 gtatctctgg tcaggctggt gcgatccgtc acggtatcac ccgcgctctg atggaatacg
    34561 acgagtccct gcgttctgaa ctgcgtaaag ctggcttcgt tactcgtgac gctcgtcagg
    34621 ttgaacgtaa gaaagtcggt ctgcgtaaag cacgtcgtcg tccgcagttc tccaaacgtt
    34681 aattggcttc tgctacggca gaaaacaatt tgcgaaaaaa cccgcttcgg cgggtttttt
    34741 tatggctaaa ttctgaatca gcgtaaaaaa tgggaagttg ttttttgctg ccgtctgaaa
    34801 gacaggtaaa acaaaaacca catcgccaat aagggtctaa gtcaactatt tcagactaaa
    34861 gcgcatctct ttttccccat ttccggcatc cactcaccac aaaggtcgca aaatctggta
    34921 aactatcatc caattttctg cccaaatgtc gggtattgct cattttttgt ttgattttcg
    34981 aacaaagaga gtcgttcctt attgggtaac acaacttctg actggccacc tggtggctgg
    35041 tagcagtaaa aattctgact atacctggag gttttcatgg ctgtcgctgc caacaaacgt
    35101 tcggtaatga cgctgttttc cggtcctact gacatctata gccatcaggt ccgcattgtg
    35161 ctggctgaga aaggtgtaag tttcgagatc gaacacgtgg aaaaggacaa tccgcctcag
    35221 gatctgattg acctcaaccc gaatcagagc gttccgaccc tggtggatcg tgagctgacc
    35281 ctgtgggaat ctcgcatcat tatggaatat ctggatgagc gtttcccgca tccgccactg
    35341 atgcctgttt acccggtagc tcgcggtgaa agccgtctgt acatgcatcg catcgaaaaa
    35401 gactggtaca cgctgatgaa cactatcatc aacggttcag cttctgaagc agatgccgca
    35461 cgtaagcaac tgcgcgaaga actgctggcg attgcgccgg tcttcggtca gaagccgtac
    35521 ttcctgagcg atgagttcag cctggtcgat tgctaccttg ctccgctgct gtggcgtctg
    35581 ccgcaactgg gcatcgagtt cagcggcccg ggtgcgaaag agctgaaagg ctatatgacc
    35641 cgcgtctttg agcgtgactc tttccttgct tctttaactg aagcagaacg tgaaatgcgt
    35701 ctgggccgga gttaatctgt atggatttgt cacagctaac accacgtcgt ccctatctgc
    35761 tgcgtgcatt ctatgagtgg ttgctggata accagctcac gccgcacctg gtggtggatg
    35821 tgacgctccc tggcgtgcag gttcctatgg aatatgcgcg tgacgggcaa atcgtactca
    35881 acattgcgcc gcgtgctgtc ggcaatctgg aactggcgaa tgatgaggtg cgctttaacg
    35941 cgcgctttgg tggcattccg cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct
    36001 acgcccgtga aaatggcgca ggcacgatgt ttgagcctga agctgcctac gatgaagata
    36061 ccagcatcat gaatgatgaa gaggcatcgg cagacaacga aaccgttatg tcggttattg
    36121 atggcgacaa gccagatcac gatgatgaca ctcatcctga cgatgaacct ccgcagccac
    36181 cacgcggtgg tcgaccggca ttacgcgttg tgaagtaata caaaacaggc ccaggcggcc
    36241 tgttttgtct ttttaatgac ttaataaaat agcgtgattg tggcaatcat attcaccacg
    36301 aaaccgactg ccatggggat cgctgtccgc ttcaccaccg caaaaggcga aacgcctgca
    36361 attcctgacg taacgaccac caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg
    36421 gcaaaatgca tcggcataat cattacaacc gctggtacat gtagtccggc tgcgatattc
    36481 ggaataagac tggcaaatga cataaacggc gcattgccaa agcccatcac aatggcacaa
    36541 atggcaatga ctagcgccat aataatcatc acgccaatac cgcccagacc agaatgctcc
    36601 gcacccctga taaccgcatc aacagtgcca atcgtcgtta agcctttcgc aaatatttca
    36661 cccgcgacca ccagcgttac cacgttgaca aactgcgtac ccatgccgtc aaaaaacgcc
    36721 tgcacatcgt ccattgtttc gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc
    36781 accgtcaaac ttagtaacat caccaccacc agatgcagtt ctgacttcat cagcccgacg
    36841 tgggcgagga acagcgagcc gagcatcagg attaacggca tcacaggtaa aatggcgtaa
    36901 tagagcggcg ggacattatc gagagttttt tgctctgcct gttcgtgatt gatattttta
    36961 tcttttttgt caaaagcgcg ttgcacgaaa aagtgggaga tcgccaccga gataatgacg
    37021 caagaggcga ccggaagctg gtagtggaag aagtatgtgg caattttcat tcccgcgacc
    37081 tgggctgcaa aaatggagtt cgtttccaga atcccccact caatggacat cgtggttgcg
    37141 ataaccgcca ccgcagagag gcgacttact cccagactca ccagcgtcgg aaataaggtg
    37201 accatcagca acatacccag accggaggcg ctggtaataa actgcgccat gatttggccg
    37261 atgacgtaag ttgccgacag aataatatac ggcgagcgaa tgagttttaa cgggcggctt
    37321 agcaagctca ccatcgcgca actggctcct atgcgctcca tg
//
LOCUS       DAJVRP010000030        36638 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVRP010000030 DAJVRP010000000
VERSION     DAJVRP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.030"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(154..1389)
                     /locus_tag="OQN12_001566"
     CDS             complement(154..1389)
                     /locus_tag="OQN12_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1653944.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1536..1612)
                     /locus_tag="OQN12_001567"
     tRNA            complement(1536..1612)
                     /locus_tag="OQN12_001567"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1576..1578),aa:Pro,seq:tgg)
     gene            complement(1655..1741)
                     /locus_tag="OQN12_001568"
     tRNA            complement(1655..1741)
                     /locus_tag="OQN12_001568"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1705..1707),aa:Leu,seq:cag)
     gene            complement(1762..1837)
                     /locus_tag="OQN12_001569"
     tRNA            complement(1762..1837)
                     /locus_tag="OQN12_001569"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1802..1804),aa:His,seq:gtg)
     gene            complement(1896..1972)
                     /locus_tag="OQN12_001570"
     tRNA            complement(1896..1972)
                     /locus_tag="OQN12_001570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1936..1938),aa:Arg,seq:ccg)
     gene            complement(2075..3460)
                     /locus_tag="OQN12_001571"
     CDS             complement(2075..3460)
                     /locus_tag="OQN12_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1653945.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3651..4391)
                     /gene="rffM"
                     /locus_tag="OQN12_001572"
     CDS             complement(3651..4391)
                     /gene="rffM"
                     /locus_tag="OQN12_001572"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1653946.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4394..5746)
                     /gene="wzyE"
                     /locus_tag="OQN12_001573"
     CDS             complement(4394..5746)
                     /gene="wzyE"
                     /locus_tag="OQN12_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1653947.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5743..6822)
                     /gene="rffT"
                     /locus_tag="OQN12_001574"
                     /gene_synonym="wecF"
     CDS             complement(5743..6822)
                     /gene="rffT"
                     /locus_tag="OQN12_001574"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1653948.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6819..8069)
                     /gene="wzxE"
                     /locus_tag="OQN12_001575"
     CDS             complement(6819..8069)
                     /gene="wzxE"
                     /locus_tag="OQN12_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1653949.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8071..9201)
                     /gene="rffA"
                     /locus_tag="OQN12_001576"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8071..9201)
                     /gene="rffA"
                     /locus_tag="OQN12_001576"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1653950.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9206..9880)
                     /gene="rffC"
                     /locus_tag="OQN12_001577"
                     /gene_synonym="wecD"
     CDS             complement(9206..9880)
                     /gene="rffC"
                     /locus_tag="OQN12_001577"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1653951.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9858..10739)
                     /gene="rfbA"
                     /locus_tag="OQN12_001578"
     CDS             complement(9858..10739)
                     /gene="rfbA"
                     /locus_tag="OQN12_001578"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1653952.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10758..11825)
                     /gene="rffG"
                     /locus_tag="OQN12_001579"
     CDS             complement(10758..11825)
                     /gene="rffG"
                     /locus_tag="OQN12_001579"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1653953.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11825..13087)
                     /gene="wecC"
                     /locus_tag="OQN12_001580"
     CDS             complement(11825..13087)
                     /gene="wecC"
                     /locus_tag="OQN12_001580"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1653954.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13084..14214)
                     /gene="wecB"
                     /locus_tag="OQN12_001581"
     CDS             complement(13084..14214)
                     /gene="wecB"
                     /locus_tag="OQN12_001581"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1653955.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14270..15316)
                     /gene="wzzE"
                     /locus_tag="OQN12_001582"
     CDS             complement(14270..15316)
                     /gene="wzzE"
                     /locus_tag="OQN12_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1653956.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15328..16431)
                     /gene="wecA"
                     /locus_tag="OQN12_001583"
     CDS             complement(15328..16431)
                     /gene="wecA"
                     /locus_tag="OQN12_001583"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1653957.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16671..17930)
                     /gene="rho"
                     /locus_tag="OQN12_001584"
     CDS             complement(16671..17930)
                     /gene="rho"
                     /locus_tag="OQN12_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1653958.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18015..18116)
                     /gene="rhoL"
                     /locus_tag="OQN12_001585"
     CDS             complement(18015..18116)
                     /gene="rhoL"
                     /locus_tag="OQN12_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1653959.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18257..18586)
                     /gene="trxA"
                     /locus_tag="OQN12_001586"
     CDS             complement(18257..18586)
                     /gene="trxA"
                     /locus_tag="OQN12_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1653960.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18717..19982
                     /gene="rhlB"
                     /locus_tag="OQN12_001587"
     CDS             18717..19982
                     /gene="rhlB"
                     /locus_tag="OQN12_001587"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1653961.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20116..21600
                     /gene="gppA"
                     /locus_tag="OQN12_001588"
     CDS             20116..21600
                     /gene="gppA"
                     /locus_tag="OQN12_001588"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1653962.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21647..23668)
                     /gene="rep"
                     /locus_tag="OQN12_001589"
     CDS             complement(21647..23668)
                     /gene="rep"
                     /locus_tag="OQN12_001589"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1653963.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23755..24036
                     /gene="ppiC"
                     /locus_tag="OQN12_001590"
     CDS             23755..24036
                     /gene="ppiC"
                     /locus_tag="OQN12_001590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1653964.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24082..25557)
                     /gene="ilvC"
                     /locus_tag="OQN12_001591"
     CDS             complement(24082..25557)
                     /gene="ilvC"
                     /locus_tag="OQN12_001591"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1653965.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25707..26597
                     /gene="ilvY"
                     /locus_tag="OQN12_001592"
     CDS             25707..26597
                     /gene="ilvY"
                     /locus_tag="OQN12_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1653966.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26594..28138)
                     /gene="ilvA"
                     /locus_tag="OQN12_001593"
     CDS             complement(26594..28138)
                     /gene="ilvA"
                     /locus_tag="OQN12_001593"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1653967.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28141..29991)
                     /gene="ilvD"
                     /locus_tag="OQN12_001594"
     CDS             complement(28141..29991)
                     /gene="ilvD"
                     /locus_tag="OQN12_001594"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1653968.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30056..30985)
                     /gene="ilvE"
                     /locus_tag="OQN12_001595"
     CDS             complement(30056..30985)
                     /gene="ilvE"
                     /locus_tag="OQN12_001595"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1653969.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31005..31268)
                     /gene="ilvM"
                     /locus_tag="OQN12_001596"
     CDS             complement(31005..31268)
                     /gene="ilvM"
                     /locus_tag="OQN12_001596"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1653970.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31265..32911)
                     /locus_tag="OQN12_001597"
     CDS             complement(31265..32911)
                     /locus_tag="OQN12_001597"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1653971.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32914..32964)
                     /locus_tag="OQN12_001598"
     CDS             complement(32914..32964)
                     /locus_tag="OQN12_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653972.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33050..33148)
                     /gene="ilvL"
                     /locus_tag="OQN12_001599"
     CDS             complement(33050..33148)
                     /gene="ilvL"
                     /locus_tag="OQN12_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1653973.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33500..35020
                     /locus_tag="OQN12_001600"
     CDS             33500..35020
                     /locus_tag="OQN12_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCS1653974.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35045..35383)
                     /gene="maoP"
                     /locus_tag="OQN12_001601"
     CDS             complement(35045..35383)
                     /gene="maoP"
                     /locus_tag="OQN12_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1653975.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35502..36341
                     /gene="hdfR"
                     /locus_tag="OQN12_001602"
     CDS             35502..36341
                     /gene="hdfR"
                     /locus_tag="OQN12_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1653976.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36437..36512)
                     /locus_tag="OQN12_001603"
     tRNA            complement(36437..36512)
                     /locus_tag="OQN12_001603"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36477..36479),aa:Trp,seq:cca)
     gene            complement(36521..36597)
                     /locus_tag="OQN12_001604"
     tRNA            complement(36521..36597)
                     /locus_tag="OQN12_001604"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36561..36563),aa:Asp,seq:gtc)
BASE COUNT         8945 a        10034 c         9229 g         8430 t
ORIGIN      
        1 caccgcacgt aaaggctttg ccggatacga tcacccaaac cagtatccac agaacgctgc
       61 aatttattga atttgcacga tcatgtaggc cggataaggc gtttacgccg catccggcaa
      121 tcaaccgcag gcggcttcca aatggccgct tttttactta ttcaccacca tcaaatgtgc
      181 ctgcataatg tcactggccg ggcgaccgtg cgccagcaaa tcagccattg ctttaagata
      241 tggcggtaga tggcggaaat aacgctgata cccggcacac aaataattca gccccggttt
      301 gccgctggca tcgagcatga agcggtgttt cgggcagcct ccccaacatg cttttaacac
      361 gttacaactg cgacactgcg ccggtaattg cttaaattta tcttcaccaa acacctgctg
      421 ttgcggggaa tcgatcattt ctgcaattgt ttgctgatgc atattcccca gccgatattg
      481 cggataaaca tagtgatcgc aggcgtaaac gtcgccgttg tgctcaacaa tcaccgagcg
      541 cccacaggtt ggctgatgat ggcaaaccgc acccggcgca ccgacaaaat tggcaaacgc
      601 ccattcgata ttcatcacga aaatcttgcc gacgtcgcgt ttgatccagt ggtcaaaaat
      661 cgccaccaga aattcaccaa attcatcggg gcgcacagac cattccgtca gttccccctg
      721 aatatcacca ggcgcatgta gtttcagtcc atcgcggaca gctgtttcat cagccaggcg
      781 ctcgaccacc ggaataaact ggatgaattc gactcccgca tcgcacaaaa aatcataaac
      841 ctgtaacggt tgctgcgcgc tggtgcggtt gacgcagacc agcacgttat agtcgacatg
      901 atgtttttgc aggagcgtca gggcacgcat caccagttta tgcgtgggtc tgccgccttt
      961 ggtcacgcga tattgattgt ggatctcagc cggaccatcc agcgataacc caacaagaaa
     1021 attattttct gccagaaatg cacaccattc gtcatcgagc agcacgccgt tagtctggaa
     1081 actgttactt atcttcctgc cagcaccata tttcgcctgt agcgccacgg cacggcggta
     1141 aaactccagc ccgagtagcg ttggttcgcc gccctgccag gtaaaagcca cttcgttttg
     1201 cagttcgctg gcggcgatat agtgacggat atacgcttcc agcgtgtcat cgtccatatg
     1261 cgtgactggc ttttcgcggt aaagggattg tttttcgaga taaaaacagt agtcacagtt
     1321 cagattacaa tcggaaccgc tcggtttcgc tatcacatga aaagcacgcg ttggaacctg
     1381 ttgcagcatg gtcgctcctt gcctgaagtc atccttcaat acgttaaggg cgaaatcata
     1441 ccgtggggca atgagttatt acgggcgttt attgcgaagg gagggtgacg aaatgcacag
     1501 aaaacaaaaa accccgccga agcggggttc aaaattggtc ggcgagagag gattcgaacc
     1561 tccgacccac tggtcccaaa ccagttgcgc taccaagctg cgctactcgc cgaaatactg
     1621 ctttttgaat ttttagttca attctttaaa gtcgtggtgc gagggggggg acttgaaccc
     1681 ccacgtccgt aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg
     1741 cattgtcaca acttctaata atggggtggc taatgggatt cgaacccacg acaactggaa
     1801 tcacaatcca gggctctacc aactgagcta tagccaccac tacaaatctt gttacgcggt
     1861 attactacca ccgcagctca agcgccggta ctaaatggcg cgcccgacag gattcgaacc
     1921 tgagacctct gcctccggag ggcagcgctc tatccagctg agctacgggc gcttagcgcc
     1981 gttgcggggg tggataatac ggacttccca cccctctgtc tagtcccttt ttaaataaat
     2041 tgctcgtttg gttacggttt gtgcgttttg ctgcttattc ctccagttta tgcgctttac
     2101 cgtggcgatt aagcccaaaa actttataaa tcgccgtcac cgccagcata aagatgatgc
     2161 cgacaaacag cgacatacgc gtatcttcat taaagtacat gccgatcaaa acgcaaatca
     2221 ggaatgccat tgttacgtag ttggcccacg ggaacaggat tgagcggaac ggatggctgg
     2281 caatcgccgc tttatgtgca cgccgaaaac gcagctggct tatcaatatc acaaaccatg
     2341 gcaccatccc cggaagcacg ctggcactgt agacgtagac aaacacacgc tgcggattgg
     2401 gaatgatgta gttcaggcat gagccaatta gcagaatagc aatagatact gccacacccg
     2461 caaccggtac gccgtgacgg gaaacttttg ccattgccgc cggtaactga cggtttttcg
     2521 ccagtgcgta gagcatacgt ccgcaactgt acatgccgct gttacagcca gagagcgcag
     2581 ccgtcagcac cacaaagttg ataatgcccg ccgctgcggt aataccgatt ttggcaaaag
     2641 tcagtacgaa cgggctgccg ttgctgccaa tttcattcca cgggaagatg gtgacgataa
     2701 cgaaaatcgc gcctacgtag aaaatcagga tccgccacag cactttgcct acggcactgc
     2761 gcagcgtcac ctgcggattc ttcgcttcac cggcagtaat gccaatcagc tccacgccct
     2821 ggtaggacgc caccacaata cacagagcgg tcaggaaccc tttccagcca cccgcaaaga
     2881 aaccgccatg ctctgtgaga ttgctaaaac caatcgactg cccgccattg ccaaagccaa
     2941 agaaaatcac gcccaggcca atgacaatca tcacgataat cgtggtgact ttgatcatcg
     3001 cgaaccagaa ctcgatttcg ccgtacaacc gcaccgccgc cagattcgcc aacgccacca
     3061 gcgccaccgc gatcaatgcg ggtatccact gcgccatctc cggggaccaa aactggacat
     3121 aaacgccaat ggcggtgatt tctgagatcc ccaccgccat ccacataaac cagtaagacc
     3181 aggcggtgag atagccaaaa aacgggctca tataacgatg cgcataaacg gcgaacgaac
     3241 cggtaaccgg ttcgaggaac aacatttcgc ccattgaacg catgatgaaa aaaacgaaca
     3301 gcccggcgat gatataggcc aacaatacgg atggcccggc ccatttcagg gtactggcgg
     3361 cccccataaa caggccgacg ccaatggtgc cccccagggc gatgagttcg atatgtcgag
     3421 cttccagccc acgctgtagc tctggtttgt tatctgccat aaatcctcgt gttgtgtttg
     3481 catgctttcc ggtgttaccg gttatcgtta tgggtacatc gagtgttgcg aatgttttcg
     3541 taattcagga gaaatggcaa ataaagcatt aaaaatttga atgctttgtg taataaaaaa
     3601 gcagacaggc gacgaagtga ccactccgtc gctttacaaa gagaggaaaa tcagaggttg
     3661 ccggtgtagt gccagcgtaa ataacgcagc aaacgaagct gacgcttaat gcggctcggc
     3721 tgcgaaagca ggcggtagag ccactccagt cccagcgttt gccagatttt cggtgcgcgt
     3781 tttacgtgac cggtgaaaac atcgtaagtc ccgccaacgc ccatatacag cgcatctgga
     3841 tgcaccagac ggcagtcgcg catgaagatc tcctgctttg gcgatcccat cgcaacggtg
     3901 acgatttgcg ctccgctggc atgaatgcgt tcaaacagcg cctgacgctg ctcgggttta
     3961 aaataaccat cctgactgcc aacgatattc acattccatt ggttgcgcag tttagcttca
     4021 gtttgcgcca gcacttcagg tttaccgccc acaagaaata ccggcgtccc ttctttgcct
     4081 gcgcgcgcca tcagctcttc ccagagatcg gcaccggcaa cgcgggaaac ctgcgcctgc
     4141 gggtactttt tacgtactga acgtacaacg ctgatgccat ccgcatattt aaattcggca
     4201 gcgttaatta actccctgac ctcggcgtta tcttcaatgg tcagcatttt ttcggcatta
     4261 atggcaacca gcgttccctg cttaagctgc ccgtcagcaa acagataatc gagggcatgc
     4321 tgcatatcac gccaaccaat caactgtaag ccacgcagcg tataggtcgg tgccgtggtg
     4381 ttgttattca ttgttatcct tcaacctgcg tccggagcga tgattttgta cgtttatgaa
     4441 tgagtccggc gctttcaaaa agccagtaca acagttttgc gatcatcaga catgcgccga
     4501 agaccacgat aaaaaagact acgcgtgaga caaacgaatc cagcccttca cgtgccagca
     4561 cgatcatatt gaagatcgcc ccaaagcaga aactgtgcaa tatcgcagcc ttatagcgat
     4621 tagtctcgcg gttgcccagc tcatacagcc agtcgaacca tttgatgatc agtccaacca
     4681 caatcgcccc gagtgggatg aacaacgcgc cgcccatcac caccagtgag cctataagcg
     4741 taggcgagat cgccagtccg gagtggttat tcagtacttc ccaggtaaag taattggctg
     4801 agttcagcac catactcggg cgccccggcc acagccagga agggataaag acatagaaat
     4861 cgcggacaat tggagccagg ccctggaagt cgatgttgtc gtagttctgc aacagcaacg
     4921 ccagattctc ccacggtgaa aaggtgtcgc gagtgagata gagaaacgta tagaacgctt
     4981 catcgccgct cacattcatt ccatagcgtt ttaacgccag ccagaacatg ccaacaatcc
     5041 ccagcacgcc cgccgccgcc agcatccaca acgaaatcca gccgcgaata atgccaataa
     5101 acaggaagat agcgaatgcg atgatgatat tggcgcgagt gccgccgaca atcatataag
     5161 tcagcaagcc aaaggcgacc gtgctgacga ggaaaaacag ccacgctttg ctgtcctggc
     5221 gcagaaagta gaccaccagc atcgccggga tgaaaaagta aaagaagcgt tttaacgcca
     5281 cgccggagac ttcactggaa aagatctgac tgtaggagtt aagccggaac agcaaaaagc
     5341 cgttgtgcat gaagaagatg ccgacgctta ccagcgcgat acccatcagg atcacccacg
     5401 taagattggt ctccacgcgg ttcatggtaa acagcggacg gcgcggtaca tcagcaacgc
     5461 gtttgcgtag gcgggttttg taggtgacat agtaaaccgc gtagaagcag cccgcagaaa
     5521 gcaacacctg caacaagatt tctggcggcg cgacaccaac atcaaagcga aataccagca
     5581 cgctggtcag cgggaagccg aagaaaaagg tgagcaaaaa cagcaatgaa aagaagacat
     5641 tgaagttaaa gcgcacacgg cgaaactcaa accaggtcag cgtggcaata aacagcgtgc
     5701 agagcagcca gaccacaaac aggccactga attgcagcag actcatgcga cctccccggc
     5761 ggcaatcgcc aacgcccgct gccagccttg tagatagtta gggctaaaga aggcaatggt
     5821 gtttttatcc gccgacgcca actggcgctg cgcttcacgc acaatatcct cgttgagatc
     5881 gtcggtagta aacagcactg gcaaatgctg ttccgtcata tcctgccaga acggattttc
     5941 cctgttaagc acacaaggaa tgcccgcctg aatcagtaag caaagcgtac caatgccctg
     6001 ctggcgggca aaaataaagt aaccaagatc gcactgacga agtagcgcca gataggcgtc
     6061 aaattccagt ttttcgctca aaatttgcag attttcttcg ctgaataact ccagccccgc
     6121 ctgacgaact tcctcaatgt acgcttcgtt attaggcgga tatcccatcg gcaccaccac
     6181 ttttaccgta tcgccaaatt gctgatgaac ggcacgtaaa gcggcgacat gctcattgct
     6241 gcggtcgccg gagttcccca ccagaatggt catttttcct tcacgttgcc gatcgttcgc
     6301 catcatattg agagaagggt ccatccgcgt cgggaagtac agcagttctc cccgtacctt
     6361 tgggtgcgtt ttggcaaaaa agctcaaatc gccgcgggtg gcaaatacac agccgactcg
     6421 cttttgcgcc aggcgacgta gtgggtaaaa aagcttatat ctcaagccac tggaaagctc
     6481 atacaggtct gccccccaga tatgccagaa aaactggctg ggcttaatgc caccactcag
     6541 cagagccagc cacagtgtgg gattgaactg accgtggaaa aaaaatcgct gctgacggtt
     6601 agcttttgct ttcgcgatga ccgcttccgc cagcgatttt ttcccaggga aaaattgcac
     6661 agaaagcgcc ggacagctat cacttaagcc gtcgtccttg ccaacaacca taaactcgcg
     6721 cgcgtgctcg ctcgtcgcgg ccagcgcgtc attgaaaaac cgcaaaacgg ttcggttatg
     6781 gtgagggata tccgatccca gtacgtgaat cagtacagtc atgcccgcct acgccagagt
     6841 aaaaacacgc cacaacaaag agaaaaatag acgatataag ttgccatata tgcctgcgcc
     6901 gcgcccagtg caccatgcgc agggattagc cagtgggcaa ataccatcaa taaagtgaac
     6961 tggctgactt ccgccagaat ataaaaccgc agtgacgctt tggcgatcac cagataacca
     7021 aagacataag cgcccacttt taacacatca cccactaact gccaggcaaa gagatcgcgc
     7081 atagcggtaa atttattcga caacagcaac cagatagcaa aatcacgcag caaccagacg
     7141 gtaaaactcg ctgccgccac tgccggtaag acgaatttca gcgatttaac cacttcccgg
     7201 gtgatatcgc gcttttccgt tagccgcgac aacgtgggca gcaaatatac gctgaacgat
     7261 gccgtaataa attgcaggta ggcatcggaa atactgctca ccccttgcca gatccccact
     7321 tcatcccagc tatactgcgc cgccagcagt ttacgcatca tgatgtaagc aacaggcaag
     7381 gtcaccgacg taatcaacgc catgagcgta aatttgctca actgccctgc cagaccgtta
     7441 tcccagctgg gtttcagata gcttaacggg atgacaccac gtttaatcaa catgatggcg
     7501 gcaggaatta ccaccagcgc gggaatcagc gccagaccca gcaacgcccc ttcataaccg
     7561 cccaaacggt aactgacgta gtacgcgaga acgccaatca agctgccgac aatcagcgat
     7621 aacgcattac ctgcggcatc gcgaaagcct ttcatcagcg ccagtaacag gttgccccag
     7681 gcgatcccca tttgcaccag cgccactaaa cgcaccagcc cctgatagtc ggtattacca
     7741 aacaatccct ggctgattgg cgcagctgcc agcacaaaaa ccagcgccat cagcgtagag
     7801 aaaccaagta ccatcgctga tgaagtgccg accacgcggc gcagctgttg cggattatca
     7861 tggtactggg caacgtattt ggttacaccg ttaaagatgc cagccccggc aagcacgccg
     7921 agcacggtaa tcaactggcg gaaatttgcc gccagcccaa gccccgccgg accaaatgac
     7981 accgccagca acttaccgac cagtaacccg gcaccaatct tgaccagtgt actggccgcc
     8041 gtccacaagg acgcttttgc caacgacata tcaggagaag tagttcaaca aagtcgcaat
     8101 taccgtacgc tgattgacgg gcgacaggtt gtagaacagc ggcaggcgca gcaggcgctc
     8161 gctctctttg gtggtgtagc gatcttcacc gtggaactca ccaaagtgtt cccccgcagg
     8221 gcaaccgtgc agcggaatgt aatgaaacac cgccatgatt tccgcttctt tcagaaagtt
     8281 aatcaacgcg ctccggtcat caatatcccg cagtttaatg tagaacatat gcgcgttctg
     8341 cacgcagcca tcgggaatcg acggcagctc gatacgcccg gctttcgcca gaggcgctaa
     8401 cgtatcgtag tagttttgcc acagcgccag acgttgctgg ttgatacgat ccgctgcttc
     8461 cagttgcgcc cacaggtacg cagcttgcag atcggacatc aaatagctgg agccaatatc
     8521 gcgccaggta tatttatcga cctgaccacg gaagaactgg ctgcggttag tgcccttttc
     8581 acggatgatc tcggctcgtt cgattaacgc tttatcgtta atcagcgtcg cgccgccttc
     8641 accacccgcc gtgtagtttt tggtttcatg gaagctaaag cagccaatat gaccaatggt
     8701 tcccagtgca cgccctttgt aagtggacat cacgccctga gcggcatctt ccaccacaaa
     8761 caaattatgc tttttcgcca acgccataat ggtgtccatt tcgcaggcca cacccgcgta
     8821 atggaccggc acgataacgc gcgttttgtc ggtgatcgct gcttcaatca acgtttcgtc
     8881 gatgttcatg gtgtccgggc gaacatccac aaagacgatt ttcgcgccac gcagcacaaa
     8941 ggcattggcg gtggagacaa aggtgtagct cggcatgatc acttcatcgc caggctggat
     9001 atcgagcagc agcgccgcca tctccagcga agcggtgcag gacggcgtca gtaacacttt
     9061 ggcgctgcca aaacgttgct ccagccactg ctggcagcga cgggtaaaac cgccatcgcc
     9121 acacagtttg ccgctaccca ttgccgactg catatagtcg agttcggttc ccaccaccgg
     9181 cggtgcgtta aatggaatca tgtgatcacc tgtataacca gtacgcggtg ctttctacat
     9241 tcgcaccact ttgtatgtat cgtttaagcg cggcggtgtt gcccatttgg gtcgccaccc
     9301 gcaaagttgt tttaccgcga gcatacgccc agtttagcgc cgtttgcatc agctcagcac
     9361 ctgcaccgcg tccagccagc aggccaattc gcgcatctgt cgcattgagt tcccgtaaag
     9421 agacatagcc gcgaatatcg ccggacgccg cacgtagaat cagacattga tgatcaaagg
     9481 tgccgcgcac ggcattttca atccactgtg cataaaagcg accgctggcg tcaggcgcat
     9541 accacggcgc acgaaaacgg ctttgcgcaa atgcggcgct agctaactga cgtaatgcgg
     9601 gaatatcggt ctcttgtgcc acgacagcac cgctatcact gacattgttc acgggtagcg
     9661 ccaaatcaac ttcaccttct accagggaga atcccagctg ttgcagggca tccagttccc
     9721 ccgtatttga tgccgcaatt ttggcctgca cccgtgacca tggcgctaag gcgtctgacg
     9781 tcaggagcgg tgcttcagac gtaatgcgca cgatggcgct gttaacacca aagaaggcgt
     9841 tttcccaggt tagtggctca atactggcgc ggacgggcac gaagtaactc cagcagatat
     9901 tggccgtagc cagttttcgc taatgaactg gcagcacgct tcacaccctc gtcatcgagc
     9961 cagccgttac gccaggcgat ctcttccagg caggcaatct taaagccctg gcgtttttct
    10021 actgtctgca caaaagtgct ggcctcaatc aggctgtcgt gagtgccggt atccagccag
    10081 gcaaatccgc gcccgagcag ttcaacggtc aggttgcccg cctcgaggta catctggttg
    10141 atggaggtaa tctccagttc accgcgctcc gacggcttca cctgctttgc gtactccacg
    10201 actttactgt cgtagaaata aagcccggtc accgcccagt ttgacttcgg ctgttttggc
    10261 ttttcttcaa gcgagatagc gcggaaattg tcgtcaaatt ccaccacgcc aaagcgttcc
    10321 gggtccatca cctgatagcc aaaaaccgtc gccccttccg tgcgcgccgc aacatgacgc
    10381 agcttcggac tgaagccctg accgaagaag atgttatcgc ccagcaccag acaagaaggt
    10441 tcaccattaa ggaaggtttc accgatgata aaggcctgcg ccagaccgtc cgggctgggc
    10501 tgttcggcat attccagctg gataccgaac tcactaccat cgcccagcag gcgctggaaa
    10561 taacctttat cttccggcgt agtgatgatg agaatttcgc ggataccggc cagcatcagc
    10621 accgacagcg gatagtaaat cattggctta tcgtaaatcg gcaacagttg cttcgatacg
    10681 ccgcgcgtaa tcggatgcaa tcgggtaccg gaaccgcccg ccaggataat acccttcatg
    10741 tttgcctccg ctgaaaatta gcctttcaga cctaaacgct caccctgata gctgccgtcc
    10801 tgcacctgct tccaccagct ttcattagcc agataccact gaaccgtttt acgcattcca
    10861 ctttcaaagg tttcctgcgg cagccagcca agttcacggg caattttcga agcatcaatg
    10921 gcataacgca gatcatgccc cggacggtca gcgacaaagg tgatcaggtc acgatagtgc
    10981 gccacgccgt gcggcttgtt cgaagccagt tcttccagca gctcgcaaat ggtttccaca
    11041 acatcgagat tcttacgctc gttgtgacca ccaatattat aggtttcacc gactttcccg
    11101 gtggtcgcca cgcaatacag cgcgcgggcg tgatcttcca catacagcca gtcacggatt
    11161 tgctgcccgt tgccatatac cggcagcggt ttacccgcca gcgcgttgag gatcatcagc
    11221 gggatcagtt tttccggaaa gtggtaagga ccgtagttat tcgagcagtt ggtgatcagc
    11281 gtcggcagac cgtaggtccg cagccaggcg cgcaccagat ggtcgctgct ggcttttgac
    11341 gcggaataag ggctgctcgg cgcatacggc gtggtttcgg tgaagaaatc atccgtcgag
    11401 tgcaggtcac catatacttc gtcggtggag atatgatgaa aacggaacgc tgatttttta
    11461 tcttccgtca gcgtattcca gtaagcccgc gccgcttcaa gcaatgtata agtcccgaca
    11521 atgttggttt caataaacgc tgccgggccg tcaatagaac ggtcaacatg gctttcggct
    11581 gccagatgca tgacacagtc tggctgatgc tcagtgaata cgcgtgccag ttctgcccga
    11641 tcgcagatat caactttctc aaaggcaaag cgctcgcttt gcgcgaccgg tgccagcgac
    11701 atcaggtttc cggcgtaggt cagcttatcg accactacca ccgcatcgct cgtttcgttg
    11761 atgatataac gcaccagcgc cgagccaata aacccggcac cacctgttat cagaattttt
    11821 ctcatcagcg ccagactcct ttggcatcga cgacatactg ctgatggaca ttgtcgccat
    11881 tgataacttt gaactgacta tgatcgacca gcatcaccag cacgtctgcc gttgccagcg
    11941 cctcgtcaag ctgcgccaga gtacaaagcc cggtcagttt tttcggcaac tggtggatgt
    12001 taggctcaac aaccagcgtt tcgccgctgt gccactgggc gatcagttca gcgatttcca
    12061 tcgccgggct ttcgcgcagg tcatcaatat tcggtttaaa cgccagacca aagcaggcga
    12121 ttttcagttc actggcgcgt ttatcggtag cagcaaggca gtcggcaacc gccgctttca
    12181 cctgatcgat aacccagaac ggtttgtgat cgttaacttc gcgcgcggta cggataagcc
    12241 gcgcctgctg ggggttctgt gccacgataa accacggatc aacagcaatg cagtgaccgc
    12301 ccacgccagg gccaggctga agaatattaa cgcgagggtg acgattcgcc aggcgaatca
    12361 gttcccagac gttaatcccc tgatcggcac aaatcagcga caattcatta gcaaaagcga
    12421 tattcacatc gcggaagctg ttttcggtga gcttacacat ttccgccgtc cgcgagttag
    12481 tgacgacaca ctcaccttcg aggaaaattt tgtacaattc gctggcgcgg gccgaacaaa
    12541 ccggcgtcat accaccaatc acgcgatcgt ttttaatcag ctcgaccata acctggcccg
    12601 gtaatacacg ttccgggcag taagcaatgt tgacgtccgc ctgctcgccc acctgctgtg
    12661 ggaaagtcag atccgggcgc atctctgcta accattctgc catcttctcg gttgacccca
    12721 ccggcgaggt ggattcaagg atcaccagcg cgcctttttt cagcactggc gcaatggagc
    12781 gagcagccga ttcaacgtag gtcatatctg gctcatgatc gcctttaaac ggcgtaggta
    12841 cagcaatcag ccaggcatcc gcttcaactg gcgtcgtgct cgctcgtaaa aaaccgcctt
    12901 ctacggcagt ttttactacg ctcgccaaat caggctcgac gatatggatt tcgccacgat
    12961 tgatggtatc aaccgcgtgt tggttgatat cgacaccaat cacctgtttt tgccgtgagg
    13021 caaacgctgc tgctgtaggc agcccgatgt aacccagtcc gataacagaa atggtcgcaa
    13081 aactcatagt gatatccgat tattttttaa cgcttccaga atgcgagagc atgcctgacc
    13141 atcaccatac gggttatggg cgcggctcat agcttgatat tcgttttcgt cttttaaaag
    13201 acgcgtcact tcctcgacaa ttcgctgctt atccgtgcct accagacgca ccgtacccgc
    13261 cgtcaccgct tccggacgct cagtggtatc gcgcatcacc agcaccggtt tccccagcga
    13321 aggcgcttct tcctgaatgc cgcctgagtc agtcaaaatc agccaggcgt ggttcatcag
    13381 ccagacaaac ggtaaatact cctggggatc gatcagaatg acatttttca catgccccag
    13441 aatgcgattg accggctctc tgacgttcgg gttgagatgc accggataga caatctggat
    13501 gtcctggtgc gtggtggcga tgtctgccag cgcgtggcag atttcttcaa agccacgacc
    13561 gaaactctca cgcctatgac cggtcaccag aatcatcttt ttatcggggg cgataaacgg
    13621 gtaatttgcc gccagttctg aacgcagcgt gtcgctgctc atcacctggt cacgcaccca
    13681 taacagtgca tcaatgactg tattaccggt aatgaagatt cggctatccg caacgttttc
    13741 acgcagcaag ttttgccggg aagtttcggt tggagagaag tgatacatcg ccagatgccc
    13801 ggtcaatgta cggttagcct cttccggcca cggcgaatag agagcgcccg tgcgcagacc
    13861 agcctcaacg tgaccaacag gaatacgctg ataaaacgcc gccaggctgg ttgccagcgt
    13921 cgtcgtcgta tcgccgtgaa ccagcacgac gtctggtttg aactcggcaa gaataggttt
    13981 tagcccttcc agaatccgac aggttatctc tgtcaggccc tgtcctggct gcattatgtt
    14041 gagatcgtag tcaggtacaa tggaaaagag tttcagtacc tgatcaagca tctcccgatg
    14101 ctgcgcagtg acgcaaactt tagcctcaaa aaaaggatct tttgccaacg catgcaccaa
    14161 cggagccatc ttgatggctt ccgggcgcgt accaaataca gtcagtactt tcacatcgat
    14221 tctcttcgaa taagcggcga gcgcctttgc gctcaccgca gcagtgttgc tatttcgagc
    14281 aacggcgggt taatgcgaca ccagccccga tcagcccccc gacaatgccc cacataatca
    14341 tcaggaaggc acgacgtggg ctatcgcgtt ttaccggttc ttccggcgta cgcaaatagc
    14401 gataggtctg aaaacgcgga tccagggttg gaccaacatt cagggtgttt aacatggctc
    14461 gattctgatc atagtcgaga tcaaaggccg gaccgacggc ctgtaaattt tccagtcgag
    14521 cctggagcat tggacgccca agcaggaaca tttctgaatc aggtaattcc tcggcaggca
    14581 catctgtcgc actgcgcgaa atattatgct gctcagcaat tttcagcgcc tgctcaatac
    14641 tgttcatccg gcggtcgtag atggctttcg ccacctcttc ctgacgcttc acctgagctt
    14701 tcatctggat ggtgcgtgcc gcccatgcgc ctttcagctc atcattcaga tggctggctg
    14761 cacgctggct ggcaaaagca acatactgac gtaacaggtt attagcgtca ggcgcggttt
    14821 cggcaataag cttcacgctg tcattgaccg cgcgagtaaa gtctccgggg ataaactgga
    14881 tgttgttaat catttcatcc agcaacgccg catcggcttt gctgttgccc accatccgct
    14941 gtttgtaata gtcggtttgc agccagaact ctctgcgggt atcccacgag gccagttgca
    15001 taacaaactc tttataggct tcgtccatga ccgatggttg atcggcagaa gccatgtttg
    15061 aacggacatc caggttacgc aaaaattgct gctgcgagta atatcccccc agcatattca
    15121 ccgttggacg atcggtaatc gccgtcgagc tccactcctg acgagcaaaa aaagtatacg
    15181 ccagcgcgat taacgcaaac gccagcccca tgccaataat ccatagcttc ccagcccaca
    15241 aggtacgaaa caacccacga atatccagtt cattttcagc gtcttcggcc ggtttcccag
    15301 gcattggttg tgtcatcaca tcctcattta tttggttaaa ttggggctgc caccacgatt
    15361 tctacgcagt ctgcgtttta cgcgcttaat aaagcgagca actttccagg cacgcttaat
    15421 gcaatatcca tagaggaaga atgctagcaa aaagagcacc agcatgaccc actccgggac
    15481 aaaatgagaa tattctgcca gcacgccaat ggaagcgagc agtgctgcgg caagggtaat
    15541 cagcacaaac gcctgacggg aagtaaaccc ggcacgcatg atcaaatggt gaatatgctg
    15601 acggtcagga gagaatgggc tcatgccttt acgcaggcga cggtacataa tcgccaccat
    15661 atccattagc ggaatggcga ttatccacaa agcggtaacc gggctgatgg gatgggtttt
    15721 gccctgggtc gtttcgagca ggatccagat aacggtaaaa ccaatcagcg tactgcccgc
    15781 atcacccata aagactttgt agcggcgacc caggatacca aggttaagca tgatgtatgg
    15841 caggatggcg gcgatcatcg caaagcacca gattgcgagg ctggtttgcc cgtcgaacca
    15901 caaaatcata ccgattgctg caaacgagac gcaggacaac ccgcccagca agccatcaat
    15961 gccatcaacc atgttgaacg cattaatggc cgcccagacg gcaaatagcg tcaggaagta
    16021 accaaacggt ccgagcacca tctcccagga gccaaagata taacccaggc tactgagata
    16081 aagtttgccg aacaccatca taacaatgcc aacagcggcc tgtatggtgg cacggatttt
    16141 tacgctgata tcaaaacgat catccagcgc gccaatgaaa acaagcacac cggcacaagc
    16201 gagatagaga gatgcatgcg gaatatagta atcgacaatt ccgaacgtga agcaaatccc
    16261 tgcgtaaacc gaaatccccc caacgagagg tatcaatccc tggtgacgtt tgcggaagtt
    16321 tggtttatcc actaaaccga ctttttttgc caccttacgg gcaaaaaaca gaaacagtgt
    16381 cgtgaataaa aaaatactga tgagatcagt actcactgtc agtaaattca caatgcatgc
    16441 tctcagagaa aattattagc agaagtataa ccacgaagac ctttattcag aagggaaagt
    16501 ccgttccgaa cccaccagcc ctgtgcaaaa atcaaccatt tgatatgcaa attgcataac
    16561 atgccagcaa attccaggaa ttttcctaac atcgccagcg cgacattaag attacagata
    16621 taaaagcaaa acgccacgta aacacgtggc gtttttggca taagacaaat ttatgagcgt
    16681 ttcatcattt cgaagaaatc gtcattggtc ttggtcatgg ccagtttatt aatgaggaat
    16741 tccattgcat cgatttcgcc catcgggtga atgattttgc gcaggatcca cattttctgc
    16801 agttcttcct gagtcgtgag cagctcttct ttacgggtac cggaacggtt gtagtcgata
    16861 gccgggaaga cgcgtttttc agcgatctta cgagagaggt gcagttccat gttgcctgta
    16921 cctttaaact cttcgtaaat aacttcgtcc attttagaac cggtatcgat aagcgccgtc
    16981 gcgataatgg tcaggctgcc gccctcttcc acgttacgcg ccgcaccaaa gaagcgtttc
    17041 ggacgatgaa gggcattggc gtccacacca ccggtcaaca ctttacctga cgccggaaca
    17101 acagtgttgt aagcgcgcgc cagacgagtg atggagtcga gcagaatgat aacgtctttc
    17161 ttgtgctcaa ccaggcgttt ggccttctcg atcaccattt ccgcaacctg aacgtggcga
    17221 gatgcgggtt cgtcaaaggt agaagcaaca acttcacctt ttaccagacg ctgcatctcg
    17281 gttacttctt ccggacgttc gtcgatcagc agaaccatca gcacacaatc cgggtggttg
    17341 taagcaatgc tctgagcaat gttctgcagc agcatagttt taccggcttt cggcggtgcc
    17401 acaatcagac cacgctgacc acgaccgata ggtgatgcca gatccagtac gcgagcagtt
    17461 aaatcttcag tagaaccgtt accacgttcc atacgcagac gagagtttgc gtgcagcggg
    17521 gttaagttct caaagaggat tttgttgcgg gcgttttcag gtttgtcgaa gttaacttcg
    17581 ttaactttca gcagcgcaaa atagcgttca ccttctttcg gcgggcgaat cttaccagag
    17641 atggtatcac cagtgcggag gttgaaacgg cggatttggc taggggaaac gtagatgtca
    17701 tcaggaccgg cgaggtagga gctgtctgcg gaacggagga aaccaaatcc atcctgcaat
    17761 atctccagta cgccatcacc aaagatatct tcgccactct ttgcgtgctg cttcaggatg
    17821 gcaaaaataa tgtcctgctt acgcatgcga gccaggtttt ccagccccat attttcgccg
    17881 agagtgatca gctcagaaac cggcgtattc tttaattcgg taagattcat agtggtgtga
    17941 gttcttaaac ttggggtaaa tctcgaactt aatgttgtga atggtatggc aggatcatcc
    18001 atgcctgttt acggtcatcg actcatgtct tttcgctgcc tggtcacagg agagtacgca
    18061 gaactgaaac gacaagacgg attgagtgac gagccagaaa tctgttcact tcgcatttaa
    18121 gataaaacgg gaagcgttgg aaattacaag attcaaactt aataaggtat gtttaatacg
    18181 aagtcaacac taagttagca tgactcacgc cgggcgtcca gtttttagcg acggggcacc
    18241 cgaacatgaa attcccttac gccaggttag cgtcgaggaa ctctttcaac tgacctttag
    18301 acagtgcgcc cactttggtt gccgccactt caccgttttt gaacagcagc agagtcggga
    18361 taccacggat gccatatttc ggcgcagtgc cagggttttg atcgatgttc agttttgcaa
    18421 cggtcagttt gccctgatat tcgtcagcga tttcatccag aatcggggcg atcattttgc
    18481 acggaccgca ccactctgcc cagaaatcga cgaggatcgc cccgtccgct ttgagtacat
    18541 ccgtgtcaaa actgtcgtca gtcaggtgaa taattttatc gctcatatat aactccacag
    18601 gaataagcct ggcgtgttgg tttcgttgtt ggtgtaacat taaccaacta aaggttgact
    18661 ttatttcacc ggatacgctt tcgtaaagca atagtaagct gatattctac cacactatga
    18721 gcaaaacaca tttaacagaa cagaagtttt ccgacttcgc cctgcatccg aaggttgtag
    18781 aagcccttga aaaaaaaggg tttcataact gtacgcccat tcaggcactg gcccttccgc
    18841 tgacgctggc gggtcgtgac gtagccgggc aggcgcaaac cggtaccggg aaaacgatgg
    18901 cgtttcttac gtcaacgttt cattatcttc tctctcatcc tgcgattgcc gatcgcaagg
    18961 tgaatcaacc gcgtgcctta attatggcac cgacgcgtga acttgccgtg cagatccacg
    19021 ccgacgcgga accgctggcg gaagctaccg gcctgaagct gggcctggct tacggtggtg
    19081 atggctatga caaacagctg aaagtgctgg aaagcggcgt tgacattctg attggcacca
    19141 cgggtcgttt aattgactac gccaagcaga accacattaa cctcggtgcc attcaggtag
    19201 tggtactgga cgaagccgat cgcatgtacg atctaggctt tattaaagat atccgctggc
    19261 tgttccgccg tatgccgcct gcaaatcagc gcctcaacat gctgttctcc gccacgcttt
    19321 cgtaccgggt acgtgaactg gcgttcgagc agatgaacaa tgccgaatat attgaagtgg
    19381 aaccggaaca gaaaacgggc caccgtatta aagaagagct tttctaccct tctaacgaag
    19441 aaaaaatgcg tttgctgcaa acgctgatcg aagaagagtg gccagacaga gcgattattt
    19501 tcgccaatac caaacaccgt tgtgaagaga tctggggcca cctggcggca gatggtcatc
    19561 gtgtcggttt gttgacaggc gatgtcgcgc agaaaaaacg tctgcgtatt cttgatgaat
    19621 ttacccgtgg cgatctggat attctggttg ccaccgacgt tgccgcgcgt ggtttgcata
    19681 ttccggcagt gacgcacgtc tttaactacg atttacccga tgactgtgaa gattacgttc
    19741 accgtattgg tcgtacaggt cgcgcaggcg caagcggtca ctctatcagc ctggcgtgtg
    19801 aagagtatgc attgaatttg cctgctattg agacctatat tggtcactca attccggtaa
    19861 gcaaatacaa tccggacgca ttgatgaccg atctgccaaa accgctgcgc ctcacgcgcc
    19921 cgcgcacagg caatggtccg cgtcgtactg gcgctccgcg taatcgtcgt cgttcaggtt
    19981 aaataaaatg tcatactcaa aatcattcaa actgtatcaa gatggcaaga gaatgaatcc
    20041 cgatgagctt actgtagtaa gtgattcggg tgaaagaatg cagccaacac agagacagat
    20101 tgaaggatga agagtatggg ttccacctcg tcgctgtatg cagccattga tctcggttcg
    20161 aatagttttc atatgctggt tgtgcgcgag gtggctggaa gcatccagac gctgacgcga
    20221 attaaacgca aagtgcgtct ggctgctggc ctgaatagcg aaaatgacct ctctaatgaa
    20281 gcaatggagc gcggttggca atgtctgcgc ctgtttgctg aacgtctgca agatatccct
    20341 ccctcgcaaa ttcgcgttgt cgctacggcg acattacgac ttgccgtcaa tgcgggtgat
    20401 tttattgcca aagcgcagga aatcctcggt tgtccggtac aggtgatcag cggtgaagag
    20461 gaagcgcgtc tgatttatca gggcgttgct cacaccactg gtggtgccga tcagcgcctg
    20521 gtggtggata taggcggtgc cagtactgaa ctggtaaccg gcacgggtgc acaaaccacc
    20581 tcgttgttca gcctgtcgat gggctgcgtc acctggctgg aacgctattt tgccgatcgt
    20641 aatctggggc aagaaaattt tgatgctgca gaaaaagcgg cacgcgaagt gttacgtccg
    20701 gttgccgatg aattacggta tcacggctgg aaagtgtgcg ttggtgcttc cggcaccgtg
    20761 caggcgttac aggaaatcat gatggcacag gggatggatg aacgcattac cctggaaaag
    20821 ttgcagcaat tgaaacagcg agccattcat tgcggtcggc tggaggaact ggagattgac
    20881 gggctgacgc tggaacgtgc attagtgttc ccgagtggtc tggcgatcct gatcgccatt
    20941 tttaccgaac tgaatattca gtgtatgacc ctggcgggcg gtgcgctgcg tgaaggcctg
    21001 gtctacggca tgttgcatct ggccgtcgag caggatattc gcagccgtac actgcgtaat
    21061 attcaacgcc gctttatgat cgatattgat caggcacagc gcgtagccaa agttgcggct
    21121 aacttcttcg atcaggtgga aaacgaatgg catcttgaag caataagccg cgatttgctc
    21181 atcagcgcct gtcagcttca tgaaatcggc ctgagcgttg acttcaaaca agctccgcaa
    21241 cacgctgctt atctggtgcg taacctggat cttcccggtt ttacccccgc acagaagaaa
    21301 ctgctggcga cgctactgct caaccagact aacccggtcg atctctcatc gctgcatcag
    21361 caaaatgccg taccaccgcg cgtcgcagaa caactctgcc gtttactccg cctggctatc
    21421 atttttgcca gccgtcgccg tgacgatctc gtgccagaga tgacattaca ggctaaccat
    21481 gaactgttga ccttgacgct tccgcaaggt tggctaaccc aacatccgct gggtaaagag
    21541 attattgctc aggaaaacca gtggcagagc tatgtccact ggccgctgga agtgcattaa
    21601 tgagtaagtg ccggatgcga tgctgacgca tcttctccgg ccttgattat ttccctcgtt
    21661 ttgccgccat catcgctttc agattcgcca gatggctttg ccctttctgc atccgttctt
    21721 cggcactgac cactttgcgc tcctgttccc aaatcagatc atcctgcggc agctccagca
    21781 aaaagcggct cggctccggg cgcaccagtt cgccgtactg acggcgttct ttacacagcg
    21841 taaaggtcaa ttccttctgg gcgcgggtaa tgccgacata ggccagccgc cgctcctcat
    21901 cgatattatc ttcatcgata ctgctctggt gcggcaaaaa cccttcttcc ataccgacca
    21961 tgtagacata aggaaactcc agcccttttg acgcgtggag agtcatcagt tgcacctgat
    22021 ccagctcttc ttcactctca ccacgctcca tcatgtcgcg taaagtaaag cgcgtcacca
    22081 cctgggtgag cgtcatcggc tcatccagtt cgctgccttc cagcatctcc gtcatccagc
    22141 taaacagttg gttgacgttc ttcatgcgca tttcggcagc tttcgtgctg ggcgatgttt
    22201 cgtacagcca ggattcataa tccatgccgt ggatcagatc gcgcaccgcg gcaatcggct
    22261 cccgctccgc cagacgctgg atttctgcca accagtgagt gaagcgggtc aatgcttcat
    22321 aaccacgtcc gctaagcgtc tgactcaggc ccatatcaaa gctggcggta aacatgcttt
    22381 tattgcgcgt catcgcccac tcacccagct ttttcagcgt cgccgggcca atctctcgct
    22441 tcggcgtgtt aacgatacgc agaaatgcgc tgtcatcgtc cgggttagtc agcacgcgca
    22501 gataagccag caagtccttg atttcaggac gagagaaaaa cgacgtacca ccagaaattt
    22561 tgtacgggat gcggttttgc atcaggaatt tttcaaacac ccgcgactga tagttaccgc
    22621 gataaagaat agcgtaatct ttgtactgcg ttttattgac gaagtgatgc gcaatcagtt
    22681 caccagtgac gcgctcggct tcatgttctt cgttattcgc gcttaaaact tttagctctg
    22741 tgccgtaacc cagttcggag aacagacgct tttcaaagac gtgcgggtta ttggcgatca
    22801 ggatgttcgc cgctttcaaa atacgtccgg aagagcggta gttctgctca agcttaatca
    22861 ccttcagcgc cggaaaatcc tggctcagca gcaccaggtt ttgcggacgt gcgccgcgcc
    22921 aggagtagat cgactggtca tcgtcaccca ccacggtaaa gcgcgcgcgg ctgcccacca
    22981 gcagtttcac cagttcatac tggctggtgt tggtgtcctg atattcgtcc accagcaggt
    23041 agcgaatttt gttctgccag cgctcgcgga cttcttcatt gcgttgcaga agtagcgtcg
    23101 gcaataaaat cagatcatcg aagtcgagca cattacaggc tttcaggtgt gcatcataca
    23161 gcccataaca atgggcaaaa atacggtccc gctcgccaat cgcactggct gccgcctggg
    23221 acggtgtttt gagatcgttc ttccagttag agatggtcga aatcaactgt tgcaggagaa
    23281 ctttgtcatc ttcaatcagc ccttcggtca actctttgag caacgccagc tgatcggtat
    23341 cgtcaaacag cgaaaagttc gccttcatcc ccagcgccgc atactcacgc ttgatgatat
    23401 ccagccccag cgtgtggaag gttgagatca tcagcccatg cgcctctttg cgacccagcg
    23461 tctgtccgac acgctctttc atctcgcgcg ccgccttatt ggtaaaggtc accgccgcga
    23521 tatgtcgcgc ctgataaccg cacccgcgga tcagatgggc gattttattg gtgataacac
    23581 gagttttacc ggaacccgca cccgccagca ccaggcaggg gccggtaacg aattcgacag
    23641 cttgttgttg gccggggttt agacgcatag gaaaatcgct caatgaaagt cgggagggga
    23701 aaataacagc gtggtagtat agccagcctc atccttacca ctcaaggcac gatcatggca
    23761 aaaacagcag cagcactgca tatccttgta aaagaagaga aactggctct ggatcttctc
    23821 gtgcagatta agaacggtgc cgatttcggc aagctggcga agaaacactc catttgccca
    23881 tcaggcaaac gcggcggtga tttaggtgaa ttccgccagg gtcagatggt tccggcgttc
    23941 gataaagttg tgttctcttg tccggtactg gagccaaccg gcccgctgca cacccagttc
    24001 ggttaccaca tcattaaggt gctgtaccgt aactaatagc aaggccttct ccgggagaag
    24061 gccttgaact ctgaatcgct attaacccgc aacagcaata cgtttcatat ccgtcatata
    24121 gccgcgcagt ttcttaccta cttgctcaat cgcatggctg cgaatcgctt catttacatc
    24181 acgcagctgc gcgttatcta ccgcaccttc cggaatagct ttacccaaat cgcccggttg
    24241 cagttctgcc ataaacggtt tcagcagcgg cacacaagcg taagagaaca gatagttacc
    24301 gtactcggca gtatcagaga taaccacgtt catttcatac agacgcttac gggcgatggt
    24361 gttggcaatc agcggcagtt cgtgcagtga ttcatagtaa gcagattctt cgatgatgcc
    24421 ggaatcaacc atggtttcga acgccagttc aacgcccgct ttcaccatcg cgatcatcag
    24481 tacgccttta tcgaagtact cctgctcgcc gattttgcct tcatactgcg gcgcggtttc
    24541 gaaggcggtt ttgccggtct cttcacgcca ggtcagcagt ttcttatcat cgttggccca
    24601 gtccgccatc ataccggagg agaattcgcc ggagatgatg tcgtccatat gtttctggaa
    24661 cagcggtgcc atgatctctt tcagctgttc agacaacgcg taagcacgca gtttcgccgg
    24721 gttagagaga cggtccatca tcagtgtgat accgccctgc ttcagcgctt cggtgatggt
    24781 ttcccaaccg aactgaatta gtttttctgc gtatgccgga tcggtgccct cttccaccag
    24841 cttgtcgaag cacagcagcg agccagcctg caacataccg cacaggatgg tttgctcacc
    24901 catcaggtca gatttcactt ccgcaacgaa ggacgattcc agcacgcccg cacggtgacc
    24961 accggttgct gccgcccatg ctttagcgat cgccatgcct tcgcctttcg gatcgttttc
    25021 cgggtgaacg gcaatcagcg tcggtacgcc gaatccacgt ttgtactctt cacgcacttc
    25081 ggtgccaggg catttcggcg caaccatgac tacggtgatg tctttacgga tctgctcgcc
    25141 cacttcgacg atgttgaaac cgtgcgagta acccagcgcc gcgccgtctt tcatcagtgg
    25201 ctgtacggtg cgcactacat cagagtgctg cttgtccggc gtcaggttaa ccaccagatc
    25261 cgcctgtgga atcagttctt cgtaagtacc cactttaaaa ccattttcgg tcgctttacg
    25321 ccaggacgcg cgcttctcgg caatcgcttc tttacgcaga gcgtaggaga tatcgagacc
    25381 agaatcacgc atgttcagac cctggttcag accttgtgcg ccacagccga cgatgactac
    25441 ttttttaccc tgaaggtagc tcgcgccatc ggcgaattca tcgcgcccca taaagcgaca
    25501 tttgcccagc tgtgccagct gctgacgcag attcagtgta ttgaagtagt tagccatggt
    25561 gattcctcgt gatgttgtgc ttcttattgt tcggttcgcg tttgcgagat tgaattcact
    25621 atatgacagg aaatttattg cggaaattga tatattcaca acgtcacatt gcaatttctg
    25681 caacgtcaac attgagggct gcgccattgg atttacgcga tctgaaaacc ttcctgcatc
    25741 tggcggaaag ccgccatttt ggccgcagcg cgcgggcgat gcacgttagc ccgtccacgc
    25801 tctcacggca gattcagcgc ctggaagaag atctcggcca gccgctgttt gtgcgcgata
    25861 accgcacggt gacgctgact gaagcgggcg aagaactgcg cgttttcgcc cagcaaacgc
    25921 tgttgcagta tcagcagctg cgccacacca tcgaccagca agggccgttg ctctatggcg
    25981 aattacatat cttctgctcg gtgaccgccg cttacagcca tctgccgccg attctggatc
    26041 gcttccgcgc ggaacacccg tcggtggaga tcaaactcac taccggtgat gcggcagatg
    26101 caatggaaaa agtcgtcaca ggtgaagctg atctggcgat tgccgggaag ccagagactc
    26161 tgcccggcgc ggtggcgttt tcgatgttgg agaatctggc agtggtgttg attgcacccg
    26221 cattgccctg cccggtgcgt aatcaggttt ctgtcgaaaa gccggactgg tcaacggtac
    26281 cgtttatcat ggccgatcag gggccggtgc gccgccgcat tgagttgtgg ttccgacgca
    26341 ataaaatcag taacccgatg atttacgcca cggttggcgg gcatgaagcg atggtatcga
    26401 tggtggcgct cggctgtggc gtggcattgt tgccggaagt ggtgctggaa aacagccccg
    26461 aaccggtgcg taaccgcgtg atgattttag agcgcagcga tgagaaaacg ccgtttgagc
    26521 tgggcgtgtg tgcgcagaaa aagcggctac atgagccgct aattgaggca ttctggaaga
    26581 ttttgccgaa ccattaaccc gccaaaaaga acctgaacgc cgggttattg gtttcgtcgt
    26641 ggcaatcgta gcccagctca ttcagccggg tttcgaaatc cggttcatgg tcgccaagtt
    26701 cgaacgccgc cagtacgcgc ccgtagtcgg tgccatggct gcgatagtgg aacaaagaaa
    26761 tgttccagtg cgtacccagc gtgttgagga agcgcagcag cgcgcccggt gattccggga
    26821 attcgaagct gtagaggcgt tcctgcaacg gatgtgacgg acgcccgccg accatatagc
    26881 gcacatgcaa cttcgccatt tcgtcgtcgg agagatccac cacgctgtag ccgccgtcgt
    26941 tgagcatctg caaaatttct ttgcgctctt cgaggccgcg gctcaggcgc acgccgacaa
    27001 agatgcaggc gtttttggca tcggcaaaac ggtagttgaa ctcggtgacc gatcgcccgc
    27061 caagcagttg gcagaatttg aggaagctgc ctttttcttc cggaatggtc accgccaaca
    27121 acgcttcacg ctgttcgccc agttcgcagc gttccgagac gtagcgcagg ccgtggaagt
    27181 tcacgttggc accggaaaga atatgcgcca gccgttcgcc gcgaatgttg tgctgggcga
    27241 tatatttttt cattcccgcc agcgccagcg cgccagaggg ttccgccacc gcacgcacat
    27301 cttcgaataa atccttcatc gccgcacaga tcgcatcgct atcgacggtg atgatgtcgt
    27361 cgagatactc ctggcataaa cggaaggttt cgtcaccgat gcgttttacc gcgacacctt
    27421 cggcgaacag tcccacgcgt ggcagatcaa ccggatgacc cgcatccagc gctgctttca
    27481 ggcaggcgga gtcttccgct tctacggcga tcactttgat ttgcggcatc agttgtttga
    27541 tcagcaccgc tacgcccgcc gccagaccgc cgccgccgac tggcacaaat acgcggtcga
    27601 gatgggcgtc ctgctggagc agttccagcg ccagcgtgcc ttgcccggca atcaccatcg
    27661 gatggtcgaa cggcggcacc caggtgaacc cctgctgctg tgacagttcg atcgctttag
    27721 ctttcgcttc gtcaaagttc gcgccatgaa gcagcacttc gccgccgaat ccacgtaccg
    27781 catcgacttt gatgtcggcg gtggcggttg gcataacgat cagggccttc acgcctaacc
    27841 gcgcagaaga aaacgcgacg ccctgcgcgt ggttacccgc agaagcagtg atcacgccgt
    27901 gcgctttttg ttcttccgtc aggcccgcca tcatggcgta tgcgccgcgc agcttaaagc
    27961 tgtgcactgg ctggcgatct tcgcgcttca ccagaatgac gttatcaaga cgcgacgaca
    28021 gtttttccat tttttgtagc ggcgtaacct gcgccgcctc gtaaaccggc gcgcgcagca
    28081 ctgctcttaa atattcggca ccttccggag caccggacag gggttgcgag tcagccatta
    28141 ttaacccccc agtttcgatt tatcgcgcac cgcacctttg tcggcgctgg ttgccaggct
    28201 ggcgtaggcg cacagggcaa aggaaacctg acgttcacgg tttttcggcg tccaggcttt
    28261 gtcaccccga gcttcctgcg cttcacgacg cgccgccagt tcggcatcgc ttacctgtaa
    28321 ctgaatgcca cggttcggga tgtcgatagc gatcaggtca ccatcttcaa tcagaccaat
    28381 gctgccgccg cttgccgctt ccggtgagac gtggccgatg gaaagaccag aggtgccgcc
    28441 agagaaacga ccgtcggtga tcagcgcaca ggctttaccg agacccattg atttcaggaa
    28501 gctggttggg tagagcattt cctgcatgcc agggccgcct ttcgggcctt catagcggat
    28561 caccaccaca tcgcccgcga caactttgcc accgaggatc gcttctaccg cgtcgtcctg
    28621 gctttcgtac actttcgccg ggccggtgaa tttgaggatg ctgtcatcga cgcccgcggt
    28681 tttcacgatg cagccgtttt cagcaaaatt accgtagagc accgccaggc cgccgtcttt
    28741 gctgtaggcg tgttccagcg agcggataca gccgtttgcg cgatcgtcat cgagagaatc
    28801 ccaacggcaa tcctgcgaga atgcctgtgt agtacgaatg cccgcagggc ctgcgcggaa
    28861 catatttttt accgcgtcat cctgggtcag cataacgtcg tattgttcca gagtttgcgg
    28921 caacgtcagg ccaagtacgt ttttcacatc acggttcagt aaccccgcgc gatccagttc
    28981 accgagaata ccgataacac caccagcacg gtgaacatct tccatatggt atttttgggt
    29041 gctcggcgca actttgcata actgcgggac tttacgcgaa agtttgtcga tatcactcat
    29101 ggtgaagtcg atttccgctt cctgcgccgc cgccagcagg tgaagtaccg tgttggtaga
    29161 gccgcccatc gcgatatcca gcgtcatggc attttcaaac gccgctttgc tagcaatatt
    29221 gcgcggcagt gcactttcgt cgttttgctc gtagtaacgt ttggtcaatt caacaatgcg
    29281 tttaccggca ttgaggaaca actgcttacg gtccgcgtgg gttgccagca gcgagccgtt
    29341 gcccggctgc gacaggccca gcgcttcggt caggcagttc attgagttag cggtaaacat
    29401 cccggagcag gagccgcagg tcggacacgc ggaacgttca acctgatcgc tctgggagtc
    29461 agaaactttc gggtctgcgc cctggatcat cgcatcaacc agatcgagtt tgattatctg
    29521 atcggacagt ttggttttcc cggcctccat cgggccgccg gaaacaaaga tcaccggaat
    29581 attcaggcgc agggaagcca tcagcatccc cggggtgatt ttgtcgcagt tagagatgca
    29641 gaccatggcg tcggcgcagt gggcgttgac catatactca acggaatcag cgatcagttc
    29701 gcgagatgga agtgaataaa gcatcccccc gtggcccatg gcaatcccat catccaccgc
    29761 aatggtgttg aactctttgg caacgccgcc agccgcttca atttgttcgg cgaccagttt
    29821 accgagatcg cgcagatgga cgtgacccgg tacaaattgg gtgaacgagt tcacaaccgc
    29881 gataatcggc ttgccaaaat cggcgtcggt cattccggtg gcgcgccaca gcgcacgagc
    29941 acccgccata ttacgaccat gagtggtggt ggcggaacgg tacttaggca tactttattt
    30001 actcccagtg tctgtctcgt aaatgggacg gtgcgtgccg tcccattttt tgtatttatt
    30061 gattaacttg atctaaccag ccccatttat cttcggtttc gccagtgaag aggccgaaga
    30121 aggcttgctg aatgcgtttg gtaaccgggc cacaacggcc ttcgccaatc tgaataccgt
    30181 ctacgctgcg cactggcgtg atttctgccg ccgtaccgga cataaagact tcatccgcca
    30241 agtacaggga ttcgcgcgac agcacctgct cacgcacttc aattcccagc tctttcgcca
    30301 gtttgatgat ggcatcacgg gtaatacccg gcagcgcgga ggaggtgaac ggtggggtga
    30361 acagcacacc atctttcact tcaaacaggt tttcgcctgc gccttcagag atataaccgt
    30421 tcacatccag cgcgatacct tcctgataac cgtggcggcg cgcttcgcta cccaccagca
    30481 gggaagagag atagttacca ccggcttttg ccgcagttgg gatggtgttt ggtgctgcgc
    30541 ggttccagga ggaaaccatc gcatcgatcc cctgctccag cgcttctgcg cccagatacg
    30601 ctccccacgg gaaagcggcg ataatcacat cggttgagta tcccgctggc gggtttactc
    30661 ccatgccaac attaccgacg aagatcagcg gacggatata ggcgctggtg agattgtttt
    30721 tgcggatcac gtcacgacaa gcttccatca gctcatcaat gctctgtgaa accgggaagc
    30781 gatagatttt ggcggagtca tgcagacgct gcatatgctc acgatggcgg aatacaaccg
    30841 gtcctttgtg tgagtcgtag caacggatgc cttcaaaaac cgaggtgcca tagtgcagcg
    30901 cgtgcgacat cacatgcacc ttcgcgtctt cccagcgaac catctcccca ttgaaccaaa
    30961 tgtaatcagc tttcttcgtg gtcattttta tattcctttt gcgctcaggc gcggatttgt
    31021 tgtgatgtgg ttgtgctctg gcagatggca acgtgtgcga cgtccaccag tttatttaac
    31081 tgactaaaca gtaagtcgac cgaccgtggg ctggcaacgg tcaattcgat atttatattt
    31141 tgtgcatcgc tggcggcagc catattcatt gagcagacgt ggaaaccacg atgacgcacc
    31201 acgcgtaaaa cacgttctaa ggtttccgga ttgaagcgag ccgatacatt gacctgatgt
    31261 tgcatcatga taatttctcc aacatttctg aattactggc acctggcggc accagcggcc
    31321 agacgttctc aagttcgtcg attgagacat gaagcaggta tggcccatca ctgttcagca
    31381 tggtgtcgag tgccgcttca acctggtctt tacgggtgat gtgttggcca gggatgccga
    31441 aggcgctggc taacatgagg aaatcggggt tatcggtaag ggtggtttcg ctgtatcgtt
    31501 cctgaaaaaa cagttgctgc cattgtcgaa ccatccctaa ccgttggtta tcgagtaaga
    31561 cgattttcaa cggtaactgc ttgcgtttta cggtgcccag ctcttgcaca ttcatcatga
    31621 aagagccgtc accggagata cagacaacgg tatcgttcgg tcgcgcgact tgtgcgccaa
    31681 ctgccgccgg taaaccaaaa cccatggtac ctaagccgct ggaggtgatg aaattttccg
    31741 ggcgagtgtg ggcgatgtgc tgcgcagccc acatctggtg ctgccccaca tctgtggtca
    31801 cgacgcaatc cgcaggttta cgatccgaca gttgttttaa caacaacgga gcgtagatag
    31861 cgtcaccggg atggtcgtaa cgccaggaat gttcatcacg cagctgcgcg cagtgttgct
    31921 gccagtcatt gatatttaac ggctgctgta atgctggtaa cagagcattt aaatcacctt
    31981 gtaatgccac atgtgcctga cgcagcttgt tcatttctgc cgggtcgata tccatatgga
    32041 taacactggc gtgtggtgcg aaggtgttca gtttgccggt cacccggtca tcaaaacgtg
    32101 cacccacggc gatcagcagg tcacactcct gcaccgcgaa gtttgccgct ttggttccgt
    32161 gcatccccag catgcccaga tagtacggat aatctgcttc tactgcgccc agccctttca
    32221 gcgtacaggt ggcaggcatt tttgtggcag cgagaaattc acgcaaagcc ggaactgcct
    32281 gcgccatccc cacgccaccg ccaacgtaca gcatcggttt ttgcgctttt gccagcatct
    32341 ggcgcgcttg ctcgacttcg gcatgtggga aagtcacttc gttttcaacg gtggtgaacc
    32401 acggttccag atcgccgctg gctaactgga tatcttttgg gatatcgacc agaaccggac
    32461 caggacgacc tgagctggca acgtcgaatg cttcagccat gatgcgcggc aactcttcca
    32521 gagactgcac cagaaagctg tgcttggtac aggctaacga caatcccagg acatccactt
    32581 cctgaaatgc gtcggtgccg ataaacggtg cggacacctg accagtgatg gcaacgacag
    32641 ggatggaatc taacagtgcg tccgcaagcc cggttatcag gttggttgcg cccggaccag
    32701 acgtggcgat acatacgcca gttttaccgg tagcacgggc ataaccgata gccgccattg
    32761 ccgcaccctg ctcatgtcgg cacagcaagt gctccacgcc gccgtcatac aatgcatcgt
    32821 aaaccggcat aattgcgcca cccggataac cgaaaacggt gttcacaccc tgtgcccgca
    32881 acgcatgtac cacccactgt gcgccattca tagttagttc cccgtcctga atcttgagaa
    32941 acagaatttt gtgctgttat tcattgtctg ctcctcggtt atgtttttaa ggtcaaaaaa
    33001 acccccggac ctttcggtgc gggggtctta gttcgttaag gcttgatctc taagcctttc
    33061 ctcgtccaag tgcagccccg cacggtggga taataatcac caccacgcta atcacgacca
    33121 ggctaatcac tcgtagaagg gctgtcattt tgtcttttct tgcatcttgt tcgaaggaat
    33181 gcctaaagag ttaccataga ttttgcgaat agcacaagat atttttgggc ctatttttat
    33241 gatggcttta ataatttcaa tagaaattgt tgttttttaa aaagaaaata gtgagtgaaa
    33301 aaattcaatt tcagtataaa tgttgttccc ttcctcgtag ttcatccttt gtccgctgca
    33361 tttttgcgag catcattcca ccgccagaac aactcaactt cacttgcaca aataaacagg
    33421 aatcagccac taaaattcac gaattagcgg gctacagcag cgatatctta tgcacataat
    33481 ggtgactcaa ggagggttta tgtcactgtc aattgttcat acccgcgcag ccctggaagt
    33541 aaatgcgccc ccaatcactg ttgaggtaca tatcagtaaa ggtctacccg gcttaacgat
    33601 ggtgggctta acagaaacaa cggtgaaaga agctcgcgat cgcgtgcgca gcgccattat
    33661 caatagcgga tatgaatatc cggcgaaaaa aatcaccatc aaccttgctc cagccgatct
    33721 gccaaaagaa gggggacgat atgatttacc tatcgccatt gcgttgctgg cggcctcaga
    33781 acagcttaca gccaataagt tagatgaata tgaattagtc ggagaactag cgcttacagg
    33841 cgctctgcgt ggcgttcccg gtgcaatctc cagtgcaact gaagctatta agtcgggcag
    33901 aaaaattatc gtcgcgaaag ataacgaaga tgaagtgggg ctaattaacg gtgaaggatg
    33961 cctgatagcc gatcatctgc aggctgtctg tgcgtttctg gaaggtaaga acactctcga
    34021 acgcccgaaa ccaactgatg cagtatcccg ggcgctacaa catgatctca gtgatgttgt
    34081 cggtcaggag caaggaaaac gaggactgga aattaccgcc gctggtgggc acaacctttt
    34141 actgattggg ccgccgggaa caggtaaaac aatgctcgcc agccgtatta atggcctgtt
    34201 gccagattta agcaatgaag aggcactgga gagcgctgcg atattaagtc tggtaaatgc
    34261 tgaatcagta caaaaacaat ggcggcagcg cccgttccgc tcacctcatc acagtgcgtc
    34321 gttaactgcg atggtgggcg gtggtgcaat tccagggcca ggtgaaattt cgttggcgca
    34381 taacggcgtg ctttttcttg atgagctacc tgaatttgaa cggcgtacac tggatgcctt
    34441 gcgagagccg attgaatccg ggcagatcca tctttcacgc acacgagcaa aaataaccta
    34501 tccagcccgt ttccagcttg ttgcggcgat gaatcccagc cctaccggac attatcaggg
    34561 aaaccataac cgctgcacgc cagaacagac attacgttat ctcaaccggc tctcggggcc
    34621 ctttctcgac cgcttcgatc tctcactgga gatcccatta ccaccccccg gcattttgag
    34681 taaaacggta gtgccgggag aaagcagcgc caccgttaaa caacgcgtaa tggccgccag
    34741 agagcgccaa tttaagcggc agaataaact gaatgcctgg ctggatagtc cggaaatacg
    34801 ccaattctgc aaacttgaga gcgaagatgc gatgtggttg gaagaaacgc tgatccatct
    34861 ggggttatcg attcgtgcct ggcagcggtt actgaaagtt gcacgaacca ttgctgatat
    34921 tgatcagtct gacattatca cacgtcagca tttgcaggag gcagttagct atcgtgcgat
    34981 tgaccgtttg ctcatccatc tgcaaaaact actgacataa aaaaagggca tttcgccctt
    35041 tttattaatc gtcagaatcg gtgtagtctt cagcaccttc aacctgcggt ttaccgccgg
    35101 aaagggtgtg aaaacgtttt ggacgcttaa tacgcgtcat atacttggac cacacgcgtt
    35161 ctgcttctgt cactggctca cgttcgccac ggcatactgc tacgaagagt ttctcttcct
    35221 cggtaaccgg ctcgcgtttg ccaagatcca actcattgaa ggcataacca tgacgctcaa
    35281 gcagttgtgc ctctttgatg gtgaaatcac catgacgaga gaatccacgt ggataatgtt
    35341 tattgtcgaa atatcgatta gtcgtcgtaa agctttccgc catcctgcac gctcctaatt
    35401 ctttgaccga gctagttatg gcgcggagta ttagttacgc ttgacagagt gtaaaacaaa
    35461 acatttaaat cataacgaca aataattttg cggagagcac tgtggatacg gaattgttaa
    35521 aaactttcct ggaagttagc cgaacgcgtc actttggtcg agcggctgaa tcgctctatc
    35581 tgacccagtc agcagtgagc tttcgaatca gacaactgga aaatcaactg ggtgtgaacc
    35641 ttttcacccg ccacagaaac aatatccgtt taaccgctgc cggtgaaaaa ctactgcctt
    35701 atgcagaaac gctcatgagc acctggcagg ccgcccgcaa ggaggtggcg catacctcac
    35761 gacataacga gttttctatc ggtgccagcg cctcgttgtg ggaatgtatg cttaatcagt
    35821 ggttgggacg cttgtatcaa aatcaggatg cccatacagg cttacagttc gaagcgcgaa
    35881 ttgcccaacg gcagtctctg gtaaaacagc tgcatgaacg ccagcttgat cttcttatta
    35941 ccactgaagc gcccaaaatg gacgaattta gtagtcagtt gctggggtat ttcactttag
    36001 cgctttatac cagtgcccct tctaaactaa agggagatct taactatctg cgactggagt
    36061 gggggccaga ttttcaacag catgaggcag gtttgatcgg tgctgacgaa gttcccattc
    36121 tgacaaccag ttctgctgaa ctggcacagc aacagattgc gatgcttaat ggttgcacct
    36181 ggctacccgt cagctgggcg cgtaaaaaag gcggcctgca taccgttgtc gatagcacaa
    36241 cactttcacg gccgctttat gccatatggc tgcaaaatag cgataaaaat gcgttgattc
    36301 gcgatctttt gaaaattaac gtgctggatg aagtgtatta atctgaatgg ctggcaagga
    36361 tgccggtaga aggatttact tcggagagga ttatttcaga taaaaaaaat ccttagcttt
    36421 cgctaaggat gatttctggc aggggcggag agactcgaac tcccaacacc cggttttgga
    36481 gaccggtgct ctaccaattg aactacgccc ctaattaggg tggcggaacg gacgggactc
    36541 gaacccgcga ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccacc
    36601 gaattctttt acaaccaccg gttttatgac cggcttac
//
LOCUS       DAJVRP010000031        36444 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVRP010000031 DAJVRP010000000
VERSION     DAJVRP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.031"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..661)
                     /gene="deoR"
                     /locus_tag="OQN12_001605"
     CDS             complement(<1..661)
                     /gene="deoR"
                     /locus_tag="OQN12_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS1653977.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RM"
     gene            complement(719..1315)
                     /gene="ybjG"
                     /locus_tag="OQN12_001606"
     CDS             complement(719..1315)
                     /gene="ybjG"
                     /locus_tag="OQN12_001606"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1653978.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1600..2826
                     /gene="mdfA"
                     /locus_tag="OQN12_001607"
     CDS             1600..2826
                     /gene="mdfA"
                     /locus_tag="OQN12_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCS1653979.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(2867..3151)
                     /locus_tag="OQN12_001608"
     CDS             complement(2867..3151)
                     /locus_tag="OQN12_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1653980.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3237..4052)
                     /gene="ybjI"
                     /locus_tag="OQN12_001609"
     CDS             complement(3237..4052)
                     /gene="ybjI"
                     /locus_tag="OQN12_001609"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1653981.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4052..5260)
                     /locus_tag="OQN12_001610"
     CDS             complement(4052..5260)
                     /locus_tag="OQN12_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1653982.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5344..5880
                     /gene="rcdA"
                     /locus_tag="OQN12_001611"
     CDS             5344..5880
                     /gene="rcdA"
                     /locus_tag="OQN12_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1653983.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6055..7740)
                     /locus_tag="OQN12_001612"
     CDS             complement(6055..7740)
                     /locus_tag="OQN12_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1653984.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8010..8387
                     /locus_tag="OQN12_001613"
     CDS             8010..8387
                     /locus_tag="OQN12_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1653985.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8417..8674)
                     /gene="grxA"
                     /locus_tag="OQN12_001614"
     CDS             complement(8417..8674)
                     /gene="grxA"
                     /locus_tag="OQN12_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1653986.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8834..9121
                     /locus_tag="OQN12_001615"
     CDS             8834..9121
                     /locus_tag="OQN12_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1653987.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9105..9827
                     /gene="nfsA"
                     /locus_tag="OQN12_001616"
                     /pseudo
     CDS             9105..9827
                     /gene="nfsA"
                     /locus_tag="OQN12_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            9888..10790
                     /gene="rimK"
                     /locus_tag="OQN12_001617"
     CDS             9888..10790
                     /gene="rimK"
                     /locus_tag="OQN12_001617"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1653988.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            10878..11354
                     /locus_tag="OQN12_001618"
     CDS             10878..11354
                     /locus_tag="OQN12_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1653989.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11706..12818
                     /gene="potF"
                     /locus_tag="OQN12_001619"
     CDS             11706..12818
                     /gene="potF"
                     /locus_tag="OQN12_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1653990.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            12913..14046
                     /gene="potG"
                     /locus_tag="OQN12_001620"
     CDS             12913..14046
                     /gene="potG"
                     /locus_tag="OQN12_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1653991.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14056..15009
                     /gene="potH"
                     /locus_tag="OQN12_001621"
     CDS             14056..15009
                     /gene="potH"
                     /locus_tag="OQN12_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1653992.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15006..15851
                     /gene="potI"
                     /locus_tag="OQN12_001622"
     CDS             15006..15851
                     /gene="potI"
                     /locus_tag="OQN12_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1653993.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            15911..16399
                     /locus_tag="OQN12_001623"
     CDS             15911..16399
                     /locus_tag="OQN12_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1653994.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16440..17567
                     /gene="rlmC"
                     /locus_tag="OQN12_001624"
     CDS             16440..17567
                     /gene="rlmC"
                     /locus_tag="OQN12_001624"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1653995.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17742..18473)
                     /gene="artJ"
                     /locus_tag="OQN12_001625"
     CDS             complement(17742..18473)
                     /gene="artJ"
                     /locus_tag="OQN12_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1653996.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18765..19433)
                     /gene="artM"
                     /locus_tag="OQN12_001626"
     CDS             complement(18765..19433)
                     /gene="artM"
                     /locus_tag="OQN12_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1653997.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19433..20149)
                     /gene="artQ"
                     /locus_tag="OQN12_001627"
     CDS             complement(19433..20149)
                     /gene="artQ"
                     /locus_tag="OQN12_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1653998.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20156..20887)
                     /gene="artJ"
                     /locus_tag="OQN12_001628"
     CDS             complement(20156..20887)
                     /gene="artJ"
                     /locus_tag="OQN12_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1653999.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(20905..21633)
                     /gene="artP"
                     /locus_tag="OQN12_001629"
     CDS             complement(20905..21633)
                     /gene="artP"
                     /locus_tag="OQN12_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1654000.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(21851..22366)
                     /locus_tag="OQN12_001630"
     CDS             complement(21851..22366)
                     /locus_tag="OQN12_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1654001.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23015..24784
                     /locus_tag="OQN12_001631"
     CDS             23015..24784
                     /locus_tag="OQN12_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1654002.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            24995..25318
                     /locus_tag="OQN12_001632"
     CDS             24995..25318
                     /locus_tag="OQN12_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1654003.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25315..26145
                     /gene="amiD"
                     /locus_tag="OQN12_001633"
     CDS             25315..26145
                     /gene="amiD"
                     /locus_tag="OQN12_001633"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1654004.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26142..27155)
                     /locus_tag="OQN12_001634"
     CDS             complement(26142..27155)
                     /locus_tag="OQN12_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1654005.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27254..28684)
                     /locus_tag="OQN12_001635"
     CDS             complement(27254..28684)
                     /locus_tag="OQN12_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1654006.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28695..29696)
                     /gene="ltaE"
                     /locus_tag="OQN12_001636"
     CDS             complement(28695..29696)
                     /gene="ltaE"
                     /locus_tag="OQN12_001636"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1654007.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29733..31451)
                     /gene="poxB"
                     /locus_tag="OQN12_001637"
     CDS             complement(29733..31451)
                     /gene="poxB"
                     /locus_tag="OQN12_001637"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1654008.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31584..32552)
                     /gene="hcr"
                     /locus_tag="OQN12_001638"
     CDS             complement(31584..32552)
                     /gene="hcr"
                     /locus_tag="OQN12_001638"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1654009.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32564..34216)
                     /gene="hcp"
                     /locus_tag="OQN12_001639"
                     /gene_synonym="priS"
     CDS             complement(32564..34216)
                     /gene="hcp"
                     /locus_tag="OQN12_001639"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1654010.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34360..35259)
                     /gene="lysO"
                     /locus_tag="OQN12_001640"
     CDS             complement(34360..35259)
                     /gene="lysO"
                     /locus_tag="OQN12_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1654011.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(35716..36411)
                     /gene="aqpZ"
                     /locus_tag="OQN12_001641"
     CDS             complement(35716..36411)
                     /gene="aqpZ"
                     /locus_tag="OQN12_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCS1654012.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9113 a         9063 c         9343 g         8925 t
ORIGIN      
        1 ccatgcgcgc cggacgcacc ttgccaaatt tactgtggtc gacaaccagc acatgctttt
       61 gcgccatcga catggcccag tgttttaccg gcaactcttc aagattaaaa caggtagcgc
      121 ctttactgac atgcacgccc gccgcggaat aaaaagcgat atccgggcaa aaattattca
      181 acgtttgctg aaaatcgatg ggtttgaaaa tggcgttgct ggcgtgaaat tcaccaccgc
      241 acagaaatgc gcggcaatgg ggtttctctt tcagcgccag aaaggtattt agcgaataac
      301 aaacggcggt aaaagggatt tcattatcaa tcgcttcaat aatccacggc gtggtggtgc
      361 cacagtcaaa aaagagggtc tgatcgggtt ctaccagtgt cgcagccagt tttgccgccc
      421 ggcgtttttc ttccaccagg cgggattttt gatcgcttaa caggtaatgg ctggcactgc
      481 gcggttccag aacaatatag ccgccgagca aaacgacggg cgcactgtgg ttgttcagat
      541 cgcgacgaat cgtcatctcc gaaaccccaa gcagggcggc ggcgtcttta agatgtaact
      601 tatcgctgcg ttttaattct tgcagcagct gcccgatacg ctcttcgcga cgtgtttcca
      661 taatccctct gaatagttat tgaagcgagc cgctcaatac tacacttttt agcagagatc
      721 agtcacgcac ccagcctttg cggatcggta atgcaaaaca gacgcgatac cacgattgca
      781 gacgttgata gagtttatgc cccatcgctt gccagataat ctgggcacta aggcagccaa
      841 tcatacctgc cagcaatcca ccgagcatat ccagcggcca gtggacgcca agataaacgc
      901 gcgaccaggc aatgacgacg gccagcacca ttaaaagtga gccggaccac aggcgatgcc
      961 agcataaaaa tgccagtgca aaggtgaaaa tcaccgtacc gtgatcgctc gggaatgagt
     1021 catccgccgc atgatgcagg aagttatagc cgatattttc gacaaagggt cggtcgtgcg
     1081 gaaaaagatg tcccatcgtc caggacacaa acaggctgac cgccagcgcg atagcgattt
     1141 ttatcaccag ttgccgttgt gctgtaagcc cccacaacca aagtaccgcg gccagcaacg
     1201 gcaccacggt aatcaaatct ttagcaataa aaatcgccaa cgagatcatc cacggagccg
     1261 agtctggcgt cgcgttaata agagagaata gagagagatt caaattttcc agcataactt
     1321 cccgacgcaa agtgattaaa agggagccaa tacaggcaag tcgttgagaa taaagtgcag
     1381 gttaaactgg gtaaagcggc atcgtcttat ttccctcaag cggcctgttt acggtgggtg
     1441 attgtaacgg gcataggtta aataaaactt aaagaaagcg tagctatact cgcaataatg
     1501 taagaatgtg cttaaccgtg gtttcagcta caaaattcgc tttctcgtta gctgcgcttt
     1561 tattaaactc tgcgcgatta ttattggcga agaaattgca tgcaaaataa attagcttcc
     1621 ggtgccaggc ttggacgtca ggcgttactt ttccctctct gtctggtgct ttacgaattt
     1681 tcaacctata tcggcaacga tatgatccaa cccggtatgt tggccgtggt ggaacaatat
     1741 caggcgggca ttgattgggt tcctacttcg atgaccgcgt atctggcggg cgggatgttt
     1801 ttacaatggc tcctggggcc gctgtcggat cgtattggtc gccgtccggt gatgctggcg
     1861 ggtgtggtgt ggtttatcgt cacctgtctg gcaatattac tggcgcaaaa cattgaacaa
     1921 ttcaccctgt tgcgcttctt gcagggcata agcttctgtt tcattggcgc tgtgggatac
     1981 gccgcaattc aggaatcctt cgaagaggcg gtttgtatca agatcaccgc gctgatggcg
     2041 aacgtggcgc tgattgctcc gctacttggt ccgctggtgg gcgcggcgtg gatccatgtg
     2101 ctgccttggg aggggatgtt tgttttgttt gccgcattgg cagcgatctc ctttttcggt
     2161 ctgcaacgag ctatgcctga aaccgccatg cgtataggcg agaaactgtc actgaaagaa
     2221 ctcggtcgtg actataagct ggtgctgaag aacggccgct ttgtggcggg ggcgctggga
     2281 ttcgttagcc tgccattgct ggcgtggatc gcccagtcgc cgattatcat cattaccggg
     2341 gagcagttga gcagctatga atatggcttg ctgcaagtgc ctattttcgg ggcgttaatt
     2401 gcgggtaact tgctgttagc gcgtctgacc tcgcgccgca ccgtacgttc gctgattatt
     2461 atgggcggct ggccgattat gattggtctg ttggtcgctg ctgcggcaac ggttatctca
     2521 tcgcacgcgt atttatggat gaccgccggg ttaagtattt atgctttcgg tattggtctg
     2581 gcgaatgcgg gactggtgcg attaaccctg tttgccagcg atatgagtaa aggtacggtt
     2641 tctgcggcga tgggaatgct gcaaatgctg atctttaccg tcggtattga aatcagcaaa
     2701 catgcctggc tgaacggggg caacggactg tttaatctct tcaaccttgt caacggaatt
     2761 ttgtggctgt cgctgatggt tatcttttta aaagataaac agatgggaaa ttctcacgaa
     2821 gggtaaaaaa atgcctgatt gatttgtgcg atcaggcatt ctcgaattaa tggtgatggt
     2881 cgtcaatctg gtgttcgata accatccctt cacctacgct ggcaagatgg cgaacataag
     2941 gatgcgggcg gtaagccgga gctggcgcag gagccacata gacggtttgc ggcacagtcg
     3001 cgacgctgac cgcttgtgga acgctgactg aatcaggaac cacaaccact ttgtatccac
     3061 tcggcacatc gacggtgaca ctttgcgcca tcgccacacc agtaaagccc attaaaagcg
     3121 cgcccaacag tagacaattt ttcataatca tttcgcctga aataatgcag cgggttaatt
     3181 tccgcgaatt atgcagcctt cattcaggca ttgtgttgcc gggcgtgctg ccgtttttat
     3241 tggtcaaatg gcgcttcgtg tttaagaact ttatcgatca catccagtac gccttcacgg
     3301 ttattgtagc ctgcccggta ttttgccgct gcgacgactg cgctgccggc attttccatt
     3361 gcaaaactaa agcctgcctg acgcagcatc tcaatatcgt taccgccatc gccaaagacc
     3421 accacttcgc tgtcgtctat tccccataat ttctgcagtt ggcgaaggcc attggcttta
     3481 tgtacgccgg ggataatcag atcgatgctg ccgttgccgg tgtggactgg caccataata
     3541 tcgccgatgg cctcatgtaa tgctttttgt acttgtggaa tcagttcatc ggaaagattc
     3601 aggccaaact taaagaaaat atcttctaag ttgtcaaagt tatcgacgta ttccagacgg
     3661 tgataataca tttccgccac cgttttcatg gcatcgtcat attttttgag tgtataggcg
     3721 ctattttttc cgcaggcaat aatttccact tccgggcgcg tcagcaaatg ttccacgaca
     3781 gtagcaaacg catccttcga cagctcgcca ttaaaaacat ctttgccttc actcattacc
     3841 cagccgccgt tttcagccac aaaggcaatt tcattcgcaa tttcagggaa gaaagagatc
     3901 aactgataat attgattccc gctggcgacc acaaagcgaa tgccttgtgc tttcatttgc
     3961 tgatactgag ccataaaccg ctcacggtta taggtttttt gatcgcttaa gaaagtacca
     4021 tccatgtcta ccgcaattaa tttaatgctc atcaactatt ctccatcgcc gtctgcgttt
     4081 tggtatcggg tttggcgacg gctttcgcga caatagccgc gagaataacc agcgccagta
     4141 caaccagcat tgcactacgt aatccataat gttcgccgag atagcccagc agcggcggcc
     4201 cgacgaggaa agccagataa ccggtcgttg ctaccacact gacgcgggtt ggtgcatcgg
     4261 ggccggtatc gctggcggca gaaatggtca gcgggaagcc cagcgaggca cccagtcccc
     4321 aaagtacaac agacactcct gcgacccagg cgctatcgac aaaaataatc agcccaatac
     4381 ccaacgcccc cattagtgca ctggcccgaa ccacggcaac gcgactgtaa ctgtcgatga
     4441 accaaccgcc ggtgaagcgt ccaacggtca tccccagggt aaaaccggca taaatcagtg
     4501 agccggaagt ggggctaaaa ccgtgaccat caaccattaa taagggtaac cagtcgttgg
     4561 cagaaccttc ggcaaaggcc atcgccagca ctacaacacc aatcaacagc aactggatat
     4621 cgcgataaaa aggaatgcct ttttcgccat gctgggtgcc atcggcagca tttttgcccg
     4681 taccgtcagg gattgcctga atggcgatat aaataggcgc gatacctacc agcgccgcca
     4741 ataaaatgtg caccgttgcc ggaacgccaa aggccgtcag tgccatcccg acaccagcgc
     4801 ctgccagcgt gcccaggcta taaaaaccgt gcatcatcgg caaaaccgtt ttattcattt
     4861 ctcgctcaac ggcggcacct tcaacgttta tcgccacttc cgcagaacca aaacttgccc
     4921 caaagacgcc gagaccaacg gcaaagagca ggggcgatgt cagccagagt gccagactta
     4981 atatcatcat cccgatcaat gcgcaggaca tcgtgaccag gatgacatta cgtgtcccaa
     5041 agcgtttcac taaccacgcc gagcagagaa taccgctcat cgaaccgatc gacagaccaa
     5101 agagaacacc gcccatttca gcgatcgaga cagagagaat atcgcggata gcaggcgtac
     5161 gggttgccca ggacgccatt aacaggcctg gcaaaaagaa gaacataaac agcgcccagg
     5221 ttcggcgttt caatgcatta cgtgaagaat ttacggtcat agatcacgtc aaaataagaa
     5281 gagggaagac aacattagca aggttgtgta catttgtaca caattgcaga cagaggaaat
     5341 gacatgcgtc gcgctaacga tccgcaacgg cgagaaaaaa ttatccaggc cacgctggag
     5401 gcggtgaaac tttacggaat acatgctgtt acgcaccgca aaattgctac ccttgccggg
     5461 gtaccgttgg gatcgatgac ctactatttt tcaggaattg atgagttgtt gctggaggcg
     5521 ttcagcagtt ttactgagat tatgtcccgg caatatcagg cattttttag cgatgttagt
     5581 gatgctcaag gcgcatgcca ggctatcacc gatatgatct acagctcaca ggttgcaacg
     5641 ccggataata tggagctgat gtaccagctc tacgcgctgg ctagccgaaa accgctatta
     5701 aaaacggtaa tgcaaaactg gatgcagcgc agtcagcaaa cgctcgaaca atggtttgaa
     5761 cccggaaccg cccgcgcgct tgatgcgttt attgagggga tgacgctgca ttttgtcacc
     5821 gaccgtaagc cgctatcgcg cgaggagatt ttgaggatgg ttgagagggt tgcagggtag
     5881 tagataaatt ttagataaca aaaaacccat caaccttgaa ccgaaatggc ggggttgatg
     5941 ggctccacaa aatggggaca tcaaagaaaa gcagtggcac taattaagac tgatgccctg
     6001 cggaaaagtt ctgcggttgt gcaaaaaaat ttcattttca gggcaacttc agttttatcc
     6061 taatcctggc cataccatga cgatgattgt ccctgccagc gtcagcagga cgttggcgat
     6121 tgcgtaggtg cccgcatagc ccagcgcagg gatgttactg cgagctgtat cactgatgat
     6181 ctccattgcc ggcgcgcagg tgcgtgcgcc catcattgcg ccgaacaaca gtgcgcggtt
     6241 cattcgcaat acataagcac cgaacaagaa acagataacc acgggcacaa gactgacaat
     6301 taatccggca atcaacatct gaccgccaat cgcgcccagg ccgttattaa taccgctacc
     6361 ggcgctcaga ccaacgcctg ccataaacac catcaagccg aactctttca ccatgcttaa
     6421 tgcaccttgc ggaatgtaac cgaaggtcgg gtggttagca cgcataaagc ccagcataat
     6481 tccggcgaat aacaacccgg cagcgttccc catgccgaaa ctgaatgtgc tgaactggaa
     6541 ggtgatcatc ccgatcatca gcccaataac aaagaaggcg caaaatgcca gcaggtcagt
     6601 gacctggctg tgaatcgaga taaagccgat gcgatcggcg atggttttta cgcgacgggc
     6661 atcaccgctg acttgtaaaa cgtcaccttt gttaagcacg acgttgtcat ctatcggcat
     6721 ctcaatctgg ctacgaatga cgcggttaag gaagcaaccg tgatcggtca acttcagttg
     6781 tgcgagacgt ttacctacag cgttatggtt tttaacgacc acttcttcag tgacgatacg
     6841 catgtcgaga aggtcacgat cgaaaacttc tttaccgtta cggaagctgg gatcgagtcg
     6901 ggcatgggcg tcgggatagc ctaccaacgc tatttcatcg cccatttgta gcacggcatc
     6961 accgtctgga tttgccagaa tcccgttacg tcgaatacgt tcaatgtagc agccggtttg
     7021 tcgataaata cccagttcac gcagattttt gccgtcggtc caggccacca gctccgggcc
     7081 gacgcgatag gcgcggatca ccggtaaata aaccttacgg ttggcatcag tgtccaggcc
     7141 acgttcgcgg gcgatttgct gggcgctggt ctgtaagtcc tgatgctgca atttcggcaa
     7201 gtaacgcgca ccaacaatca aactcaccag accgattaaa taggttaagg catacccgag
     7261 gctcagatta tccagtgcca gtgagagctg cctgctttcc atgccggaat gatgcagtgt
     7321 atcgccagca ccgaccagaa ccggtgtcga cgtcatagag cctgctaaca taccggccgt
     7381 caggccaata tcccagccaa acagcttacc taaccctaag gcgatcacca gcgcactgcc
     7441 aaccatcacc agtgctaaca ttaggtaatt tttcccatca cgaaaaaaaa tggaaaaaaa
     7501 gttcggtccg gcttcgaccc cgacgcagaa aataaacagc ataaagccaa gattaagcgc
     7561 atcggtgtta atgctgaaat gttgttggcc taataacagc gatacgacta aaacgccaat
     7621 ggaattaccc agttggatcg aaccaagtcg taactttccg agacatagcc caagcgcgag
     7681 gaccacaaat aataacagaa tgtaattccc atttaacaat tcggcgacgt ttatattcac
     7741 ggaggctaac ttcttgttta ctagtaagct gttgaaagaa atggtaattt acgataatgt
     7801 gttttaccag aattcagggc gcagattcat tcagcgcacc taaacgatag taaagtaaca
     7861 atatatttta ctagtgtaat cacattaggt atcaacggct atatgaattg cgttggccta
     7921 tattagcatg gaatgcgaag cggctttatc ttactgaacg ccacactggc gaaaaatgtg
     7981 ttcgatagac gcagtgtcag gaggaacgag tgaaacataa acaacgttgg gcgggggcaa
     8041 tctgctgttt tgtcctcttc attgtggtgt gcctttttct ggcgacgcac atgaaaggcg
     8101 cttttcgggc tgccgggcat cctgaaatcg gcttgctgtt tttcattctt cctggagcag
     8161 ttgccagttt cttttcacag cgtagagaag tcctgaaacc tctatttggc gcaatgctgg
     8221 cggcaccctg ttcaatgctc attatgcggc tgtttttttc accgacgcgc tcattctggc
     8281 aagagctggc atggttacta agcgcggtgt tctggtgtgc gctgggggca ctgtgtttct
     8341 tatttatcag tagtttgttt aaaccacagc acagaaaaaa tcagtaaagc cctcaacgcg
     8401 agggcttgtc agacgatcag gcgtccagat tttctttcac ccatgcagca aaatcggtat
     8461 agccgccgat atgttgctga tcgacaaaaa tctgcggcac ggtttctacg ggtttacctg
     8521 ccttttgttg tagatcttct ttagtgatcc cttccgcacg aatatctaca tactgatact
     8581 gaaaatcatc gcgttcattg ctcaatttct cagccagatc ttttgcacgc acacagtaag
     8641 ggcaacccga acgaccaaaa ataacggttt gcattatttc tctcctcata gatttatgcc
     8701 tgtaatgatc acgctaaaat gtattcgctg aaagtaggtt taacctgttg cattaattgc
     8761 taaaagctat aactgttaaa cacaatacag tgaaaagttt tagactgaag gctcactttg
     8821 cagagggaag cgtatgcgcg cgatcggtaa attgcctaaa ggcgtgttga tactggaatt
     8881 tatcggaatg atgctactgg cggtggcgct gctgtcggta agcgactccc tgtcgctgcc
     8941 tgagccattt tctcggccag aagtgcagat tctgatgatt tttctcggtg ttttgctcat
     9001 gcttcccgct gcggtggtgg ttattcttca ggtggcaaaa cgtcttgccc cacagctgat
     9061 gaaccgtcca ccgcaatatt cacgttcaga aagagaaaaa gataatgacg ccaaccattg
     9121 aacttatttg tggccatcgc tccattcgcc atttcactga tgaacccatt tccgaagcgc
     9181 agtgtgaggc gattattaac agcgcccgtg cgacgtccag ttccagtttt ttgcagtgta
     9241 gtagcattat tcgcattacc gacaaagcat tacgtgaaga actggtgacg ctgaccggcg
     9301 ggcaaaaaca cgtagcgcaa gcggcggagt tctgggtgtt ctgtgccgac tttaaccgcc
     9361 atttacagat ctgtccggat gctcagctcg gcctggcgga acaactgttg ctcggtgtcg
     9421 ttgatacggc aatgatggcg cagaatgcat taaccgcagc ggaatcgctg ggattgggcg
     9481 gggtatatat cggcggcctg cgcaataata ttgaagcggt gacgaaactg ctgaaattac
     9541 cgcagcatgt tctgccgctg tttgggctgt gccttggcta gcctgcggat aatccggatc
     9601 tcaagccgcg tttaccggcc tccattttgg tgcatgaaaa cagctatcaa ccgctggata
     9661 aagacgcact ggcgcagtat gacgagcaac tggcggaata ttacctcacc cgtggcagca
     9721 ataatcgctg ggatacctgg agcgatcata tccgccgaac aatcattaaa gaaagccgcc
     9781 catttattct ggattatttg cacaaacagg gttgggcgac gcgctaaaac cgctacgtcg
     9841 atgtatgata cgcgggcttt tgaccaggtc tgacagagag gtgcagggtg aaaattgcca
     9901 tattgtcccg ggatggaacg ctctattcgt gtaagcggct gcgtgaagcc gctatacagc
     9961 gcggtcacct ggttgaaatt cttgatccgc tttcttgcta catgaacata aatcctgcgg
    10021 cgtcttctat tcactacaaa ggccgcaagt taccccattt tgacgcagtg atcccgcgta
    10081 ttggcaccgc cattaccttt tatgggacgg cggcactgcg ccagttcgag atgctgggga
    10141 gctatccgct caatgagtcg gtcgccattg cccgggcgcg tgacaaattg cgttccatgc
    10201 aactgctggc gcgtcagggc atcgacctgc ctgtcacggg cattgcgcat tcgccggatg
    10261 ataccagcga tttaatcgac atggtcggtg gtgcgccgct ggtggtcaag ttggttgaag
    10321 gcacgcaggg aattggcgtc gtgctggcgg agactcgtca ggcggcggaa agcgtgattg
    10381 acgctttccg cggtctgaac gcgcatattc tggtgcagga atatatcaaa gaggcgcaag
    10441 ggtgcgatat ccgctgtctg gttgttggtg atgaagtggt cgctgcgatt gaacggcggg
    10501 cgaaagaggg ctattttcgt tccaatttgc atcgtggcgg cgcggcaagc gtcgccagta
    10561 tcacaccaca ggagcgtgaa atcgcgataa aagccgcgcg aacgatggcg ctggacgttg
    10621 ctggtgtgga tattctgcgt gctaatcgcg ggccgttggt gatggaagtg aatgcgtcgc
    10681 cggggctgga aggaatagaa aaaaccaccg gtatcgatat cgcgggtaaa atgatccgct
    10741 ggatcgaacg ccacgctacg acagaatatt gcctgaaaac gggtggttag tcgcaatcac
    10801 attactgatc atggttttgc ctgcgctttt tgcgtaagct gtgccggtct ttttatcgaa
    10861 agaggttgta caaaattatg acatcgctgg tcgttcctgg tctggatacg ctgcgtcaat
    10921 ggctcgatga cctggggatg agtttttttg aatgtgataa ctgtcaggct ctgcatctgc
    10981 cccatatgca gaatttcgac ggtgtctttg atgccaaaat cgatctgatc gataacacga
    11041 tcctgttttc tgccatggcg gaagtccgac cttcagccgt attgccgctg gcggcggatt
    11101 tatctgccat caatgccagt tcgctgaccg tgaaagcatt tcttgatatg caggatgata
    11161 atctgccaaa gctggtggtt tgccagtctt tatccgttat gcagggcgta acctatgagc
    11221 agtttgcatg gttcgtgcgt cagagcgaag agcagatttc gatggtcatt cttgaagcta
    11281 atgcccatca actgctgtta ccgactgacg atgaagggca aaacaacgtt accgaaaact
    11341 attttctcca ctgataattc ctttcgagca cgcagtcgct gctgcagtga ctgcgcgctg
    11401 caaaattatc tgctgttttt aaccttttct taaagattat ttcacttctc ttgtgtcgat
    11461 ttggctttat cacatagagc aaatacgcat aaaaatttgt taaataccgt tttttaatcc
    11521 gagctatagt ctcaaaccct ggctaaagtt attcttgcga tgcttctata tagcgagcag
    11581 tgctggccgg gagagagttc tcttttctta caccgcgccg ataaaaaaat atgcacgttt
    11641 attgcatatc tttcagtgtg acaacttttg ttcgtttgtt aacgaacttt cagaaggaaa
    11701 gagatgtgac cgccttaaat aaaaaatggc tatcgggtct ggttgcgggt gctctgatgg
    11761 ccgtctctgt cggcacgctc gcggctgaac aaaaaacact ccacatttat aactggtctg
    11821 attatatcgc ccctgacacg gtggccaatt ttgaaaaaga aaccggtatt aaagtcgtct
    11881 acgatgtttt cgactctaac gaagtactgg aaggcaaatt aatggcgggt agcaccggct
    11941 ttgacttggt ggttccatct gccagctttc tagaacgcca gttgactgcg ggagttttcc
    12001 agccgctgga caaaagcaaa ttgccggagt ggaagaatct cgacccggag ctgctgaagc
    12061 tggtcgccaa acacgatccc gacaataaat ttgctatgcc ctatatgtgg gcgacgaccg
    12121 ggattggcta taacgttgat aaagttaaag ctgtgctggg cgaaaacgcg cccgtcgata
    12181 gctgggactt gatcctcaaa cctgaaaatc tggaaaaact gaaaagctgc ggtgtctctt
    12241 tcctggatgc gccagaagaa gtttttgcta ccgtgttgaa ttatctcggc aaagatccca
    12301 acagcactaa agcggatgat tacaccggac cggcaaccga tctgctgtta aagctgcgcc
    12361 cgaacattcg ttatttccat tcatctcaat acattaacga cctggcaaac ggcgatattt
    12421 gcgtcgctat cggctgggca ggtgatgtct ggcaggcgtc aaaccgcgcg aaggaagcga
    12481 agaatggagt gaatgtctcg ttctcgattc caaaagaagg ggcgatggcg ttctttgatg
    12541 tattcgccat gcctgcggat gccaaaaaca aagacgaagc ctatcagttc ctgaattacc
    12601 tgctgcgccc ggatgtagta gcgcatattt ccgaccatgt gttctatgtc aacgccaata
    12661 aagcagccac gccgctggtg agtgcggaag tccgtgagaa cccgggtatt tatccgcctg
    12721 cggatgttcg tgcgaagctg ttcactctga aagtgcagga cccgaaaatc gaccgtgtgc
    12781 gcacccgcgc gtggaccaaa gtgaagagcg gaaaataatc cgcagcctta gatgccgggc
    12841 gggcgcacca cacccgccgg caattcgcac catcatggtg cgcttgcaca cattcaatgc
    12901 cggagagcag ccgtgaatga cgctatccct cgcccgcagg cgaaaacccg taaggcgctg
    12961 acgccgctat tagaaatccg caacctcacc aaatcctacg atggtcaaca tgcggtggat
    13021 gatgtcagcc tgaccatcta caaaggtgaa atcttcgcgc tgctgggcgc atccggctgt
    13081 ggcaagtcca cgctgctgcg tatgctggca ggtttcgaac aaccttctgc cggacagata
    13141 atgcttgatg gcgtcgattt gtcacaggtt ccgccttacc tgcgccccat caatatgatg
    13201 tttcagtctt acgcgctgtt tccgcatatg accgtggaac agaacatcgc ttttggcctg
    13261 aaacaggaca aactaccgaa agcggaaatt tccagtcggg tcaatgagat gctcggcctg
    13321 gtgcacatgc aggagttcgc caaacgtaaa ccgcatcagc tttctggtgg tcagcgacag
    13381 cgtgtggccc tggcccgaag ccttgccaag cgcccgaaac tattactgct cgacgagccg
    13441 atgggcgcgc tggataaaaa gctgcgtgac cggatgcagc ttgaagtggt ggatattctg
    13501 gagcgcgtcg gtgtgacttg tgtgatggtc acccacgatc aggaagaggc gatgaccatg
    13561 gcggggcgca tcgccattat gaatcgtggg aaatttgtcc agattggcga accggaagag
    13621 atctacgagc atccgactac ccgctatagc gccgaattta ttggctcggt aaacgtcttt
    13681 gaaggcgtac tcaaagagcg tcaggaagat ggcctggtgc ttgattcgcc ggggctggtg
    13741 catccactga aagtcgacgc ggatgcctcg gtggtcgata acgtgccggt acatgtggcg
    13801 ctgcgcccgg aaaaaatcat gctttgcgaa gagccgcccg ccaatggttg taacttcgcg
    13861 gtgggggagg tgatacacat tgcctatctc ggcgatcttt cggtgtatca cgttcgtctg
    13921 aaaagtgggc agatgattag tgcccagcta caaaacgccc atcgtcatcg taaagggtta
    13981 ccgacctggg gcgacgaagt gcgtttgtgc tgggaagtgg acagctgtgt ggtgctgacg
    14041 gtttaaggag caaagatgaa tacacttgaa cctgctgccc agtcgaaacc gccgggcgga
    14101 tttaagctgt ggttgtcgca gctgcaaatg aagcatgggc gcaaactggt cattgcgttg
    14161 ccatatatct ggttgatctt gctgtttctg ctgccatttc tgattgtctt taaaataagc
    14221 ctggcggaga tggcgcgcgc tattccacct tataccgaac tgatggagtg ggctgacggg
    14281 caactgtcca tcactcttaa tctcggtaat tttctgcaac tgaccgacga ttcgctctat
    14341 ttcgatgctt atctccagtc gctacaggtg gctgcgattt cgacattttg ctgtttactg
    14401 atcggctatc cgctggcgtg ggcggtggcg cacagtaagc cttcgacccg taatatttta
    14461 ttactactgg tgatcctgcc gtcgtggacc tcgtttctga tccgcgttta tgcttggatg
    14521 ggaatattaa aaaacaacgg tgtgctgaat aattttctgc tgtggctggg ggttatcgat
    14581 caaccgctga ccattcttca taccaatctg gcagtttata ttggcattgt ttacgcctac
    14641 gtgccgttta tggtgctgcc gatttatacc gcgttgattc gtattgatta ttcgctggtg
    14701 gaagcagcgc tggatctcgg tgcacgaccg ctgaaaacgt tctttactgt gatcgtgccg
    14761 ctgactaaag gtgggattat tgccggatcg atgctggtgt ttatcccggc tgtgggcgag
    14821 tttgtgatcc cggaactgct cggtggcccg gacagcatca tgatcgggcg cgtgctatgg
    14881 caagagtttt tcaataaccg cgactggccg gtggcctcgg cggtagcgat catcatgttg
    14941 ctgctgctaa ttgtgccgat aatgtggttc cacaaacacc agcaaaaaag cgtgggagaa
    15001 cacggatgaa taatttaccg gtagttcgtt cgccctggcg gattgtgatt ttgctgctgg
    15061 gctttacctt tctctacgcg ccaatgctga tgctggttat ctattcgttt aacagctcga
    15121 agctggtgac ggtgtgggcc ggctggtcaa cgcgctggta tggtgagtta ttgcgcgatg
    15181 acgcgatgat gagtgcggtt ggttcaagcc tgacaattgc tgcctgtgcg gcaacggcgg
    15241 cggcgatcct cgggactatt gcggcggtgg tgctggtgcg ctttggcagg tttcgcggat
    15301 caaatggctt tgcctttatg atcaccgcgc cgctggtaat gccagatgtc atcacgggct
    15361 tgtcgctgtt gttattattc gtcgcgcttg ctcatgccat tggctggcct gcggaccgcg
    15421 gtatgcttac catctggctg gcgcatgtca ctttctgtac ggcttatgtg gcggtcgtta
    15481 tttcgtcgcg tctgcgggaa ctggatcgct cgatagaaga agccgcgatg gatctcggtg
    15541 cgacgccgct gaaagtgttt ttcgtcatta cgctaccgat gatcatgccc gcgatcattt
    15601 ctggctggtt actggctttt actttgtcgc ttgatgatct ggtgatcgcc agctttgttt
    15661 ccgggccggg agccaccacg ttaccgatgc tggtcttttc cagcgtgcgg atgggggtga
    15721 atccggaaat caacgccctg gcaacattga tcctcggcgc ggtcggaatt gtcggattta
    15781 tcgcctggta tctgatggcg cgcgcagaaa aacagcggat acgcgacatc caacgtgcaa
    15841 gacgtggctg aagacactaa aatttgccaa cctggctaca taatgccgcg catgtcgcgg
    15901 cattgttttc atggaagacg aaacgttggg attttttaag aaaacatctt catctcatgc
    15961 tcgcctgaat gtgcctgcgc tggtgcaggt ggcggcgctc gccattatta tgatccgtgg
    16021 cctcgacgtg ctgatgattt tcaatacgct gggcgtgcgc ggtattggcg agttcattca
    16081 tcgcagcgta caaacctgga gtttaacgct ggtcttttta agcagtctgg tgctggtttt
    16141 cattgagatc tggtgtgcgt tttcactggt gaaagggcgt cgctgggcgc gctggctata
    16201 tctgctgaca caaatcaccg ccgcaagtta cttgtgggcg gcttcgctgg ggtacggtta
    16261 tccggagctg ttcagcattc ccggtgaatc aaaacgtgaa atcttccata gcctgatgct
    16321 gcagaagctg ccggatatgc tcatcctgat gctgctgttc gttccctcga ccagtcggcg
    16381 gttcttccag ttgcaataat gtgtataatc gtcgcccctg atgatgtgaa ggtcaatgta
    16441 tgcagtgcgc actttacgac gcgggtcgct gtcgttcctg tcagtggata acgcagccga
    16501 ttccagagca actctccgct aaaaccgccg atcttaaaaa tctgctcgcc gattttccgg
    16561 ttgaggaatg gtgcgcgccg gtgtcaggcc cggaacaagg gtttcgtaat aaagccaaaa
    16621 tggtggtgag tggtagcgtt gaaaaaacac tgctcggtat gctgcatcgc gatggcacgc
    16681 cggaagacct ttgtgactgc ccgctttatc cagcctcatt tgcgcccgtt tttgcggcgc
    16741 taaaaccgtt tatcgcccga gcggggttaa cgccctacaa cgtggcgcgt aagcgtggcg
    16801 aactgaaata cattctgctg actgaaagcc agagtgatgg cggcatgatg ctgcgctttg
    16861 tactgcgttc tgataccaag ctggcgcaac tgcgtaaggc gctgccgtgg ttacacgaac
    16921 aactaccgca gctgaaagtt attaccgtca atattcagcc ggtacatatg gcgattatgg
    16981 aaggggagac ggagatctac ctgaccgaac aacaggcact ggcggagcgt tttaatgacg
    17041 taccgctgtg gatccgtccg caaagtttct tccagactaa tccggcggtc gccagccagt
    17101 tgtacgccac cgcgcgcgac tgggtacgac agctgccggt taaacatatg tgggatctgt
    17161 tctgcggtgt ggggggcttt ggtttacact gcgcgacgcc tgacatgcag ttaaccggga
    17221 tcgaaattgc gccagaggcc attgcctgtg caaagcagtc agccgctgaa ctgggcttaa
    17281 ctcgtttaca atttcaggcg ctggactcca ctcagtttgc cactgctcag ggggaggtgc
    17341 cggagctggt gctggttaac ccgccgcgcc gcggcattgg taaaccgctg tgtgattatc
    17401 tttcaacgat ggcaccgcgt tttattatct actccagctg taacgcccaa accatggcga
    17461 aagatatccg cgaactgcca ggttaccgca tcgaacgggt acagcttttt gatatgttcc
    17521 cgcacaccgc gcactatgaa gtgttgacgc tgctggtgaa gcaataaaaa agccacaggt
    17581 gcggcttcag attgctgatc aggtgcgctt tgttcatgcc ggatgcggcg tgaacgcctt
    17641 atccggccta caaaatcatg ctaaatcaat acattgcaga gttgatgtgg gccggataag
    17701 cgcagcgcat caggcaatct tacttttgtc atcagtctca attactgcgg aaaccactgg
    17761 tcattgattt tctgatatgt accgtcagct ttaattgctg ccagcgcgtt attcagtttt
    17821 tccagcagag ctttgttatc cggacgtaca gcgataccca ggccggtgcc gaagtattgc
    17881 ggatcggtca ctttttcagt cgcaacgccc agttgcggat tggttttcag ccattcgttt
    17941 accaccgcgg tgtcaccaaa taccccatca atacgaccat ttttcagatc gataaaggca
    18001 ttctgataac tgtcatagga gacagttttc acttccgggt gctgatcctg aatatatttc
    18061 tggtgcgtcg taccgttttc catcccaatg cgtttgcctt tcaggtcggc aaacgttttg
    18121 taggtatctt ttttggaaat cacgacggct gagttttcat agtagggtgt ggtaaacgaa
    18181 acctgcttgc tacgctccgg ggtgatatcc ataccggaga ttacggcgtc atattttctg
    18241 aatttcagtg acgggatcag gctgtcgaac gcgtgattag taaaagtaca ttctgcctgc
    18301 atttgtttgc acaaggcttt tgccagatcg atatcaaagc cgacaatctc attattagca
    18361 cctatagatt caaagggtgg ataggtggct gaaacgccaa aattgatttt ctctgcggca
    18421 gaagcaccga aagtaaagga agcaagtaaa gcggcaagaa ctaacttttt catgatggaa
    18481 ctcccgtctg tcaatcttat gatttttggc cgtgtctgcg gcatgggata acaatgccat
    18541 caagtgaatt tatatgcaat aaacatgatt aaataattta aatgaaataa aaaaagacgg
    18601 ataacttagt gagttgtccg tcttcattat aagaatttat gcactatgta ggccggataa
    18661 ggcgtccacg ccgcatccgg cacaggcacc gtgctgatgt ctgatgcgac gctggcgcgt
    18721 cttatcagac ctacaaaccc ccccggcgaa tgtacgcagc tacattaatt ccgccgttcg
    18781 aatgccagtg ctttgcgctc gatcagacgc atcatcagcg tcagcaggcc gttgacgacc
    18841 aggtaaataa tccctgccgc accgaacacc attacatcgt aggtgcgtcc gtacaacaac
    18901 tggctgtatc ccatcacttc catcagcgta atggtgtaag ccagagaggt acttttgaac
    18961 accagcacca cttcgttgga ataagaagag agcgagcgtt taaaggcata cggcagcagg
    19021 atcgccagcg tatctttttt gctcattccc agggcgctac aggactgcca ctgaccttct
    19081 gggatcgcac gaattgcacc gtaaaacagc tgcgtggtat acgccgcact gttcagcgat
    19141 aacgcaatca gcgcacataa ccacggttct gacaacaaat gccacagtgc cggatactcc
    19201 tgcaacgtcg gaaattggcc cggcccgtaa taaatcagga agatctgcac cagcagcggc
    19261 gtaccggtaa acagcgtgat ataaccccgc accagccaca ccagcaccgg cgttttcagc
    19321 gtcaggatga tggtaaaaat caatgccaga atcagtgcca caatcagcga ggcaacggtt
    19381 agcgtcaggc tggtgtgcag ccctttcatc agctcgggta aatactcaaa cattagctgg
    19441 gcctccgctc aaaacgtgtc gcgcgcaggt caatgcgttt gagaatgtac tgactgagca
    19501 gggtgatcac caggtaaatc gccgccgcca caatgtacca ggtaaatggt tcctgggtac
    19561 gagtagcaat gcttttggtt tgtaacatta aatcattcac actaatcaaa ctgaccagcg
    19621 cggtatcttt cagcagcacc agccactggt taccgaggcc aggcagcgca tgacgccaca
    19681 tctgcggcat caccagacgg aaaaagatag ccgatttcga cagccccagc gcctgaccag
    19741 attcccactg gcccaccggt acagctttca acgcgccgcg cagcgtttgc gaggcatagg
    19801 cggcatacag cagtgacaga gcgatgacac cacaaaggaa cggactcacg tcgaagttct
    19861 caatgtccat ctgcaccggg atctgcacga acccaagatt gatagtgaag ccatccgaaa
    19921 gcgtcagcag taactgcgag gagccaaaat agataaacag cactaccaga atttctggca
    19981 ggccacgcag aatggttacc agcgctgaac ctgcccacgc gacaggacgc catttcgctg
    20041 actcccatac cgcaaagaac atcgccagcg ccagcccgac aatcaatgca caaacggcaa
    20101 ggccgacggt catcccggcg gcgcttgcta aaggaaaaaa ttcattcatc aggaattact
    20161 tctggaacca tttgttgtag atggtttcgt aagtgccatc tttcttcact ttttccagcg
    20221 cagtgttgag tttctgctgc agttcagtgt tgccctgacg taccgcgatg ccgaggccag
    20281 taccgaaata atctttatcg gtcactttat cgcccactgc cgccagtttt gggttatctt
    20341 tcagccactc agtgaccact gcggtgtcac caaatacgct gtcgatacgc ccgttttgca
    20401 gatccagttt tgcgttctgg tagctgtcat acggaacggt ggtgatttcc gggtgcttat
    20461 ccataatgaa cttctggtgt gtcgtcccgt tctgaacgcc gacttttttg cctttcagct
    20521 gatcaacact ggtgtatttg ccttgctgac ccacaaacag ggcagagttg tcatagtacg
    20581 gggtagtaaa cagtacctgc ttttcacgct ccggagtgat atccatgccc gccatcactg
    20641 cttctacgcg acggaatttc aggcttggga tcaggctgtc aaacgcctgg ttagagaaag
    20701 tacaggttgc atcaatctct ttacacagtg cctgtgccag gtcgacgtca aaaccaacga
    20761 tctggttgtt tgcatcaatc gattcaaacg gaggataggg ggcttcggta gcaaaacgaa
    20821 tggtttcggc agctgtggcg gaaagactaa aacctgcaat taacgcggca atcagaactt
    20881 ttttcattgt tgttatcccg aatcttagtg agagagatag tttttaaatg cttcggtttg
    20941 cggctcggta aagcagctcg cgtcgccttg ttctacgata tgaccatttt ccatatacac
    21001 cactcggctg gcggttttac gcgccacttc aacttcgtgg gtgacgatca cctgggtaat
    21061 attcgtttct gccagctcac gaatgatgct gacgatttgt gccgtaattt ccgggtccag
    21121 tgcggcggtc ggttcatcga acagcagtac ctgcggttcc atcatcaacg cacgggcaat
    21181 agcaacacgc tgctgctgac caccagaaag atgcagcggg taacgatcgc tataaggttt
    21241 gagacgcaga cgttccagca gtttttctgc acgggccagc gcctgatctt tactcaaccc
    21301 cagtacacgg cagggcgctt caatcaggtt ttgctgcacg gtcagatgcg gccacaggtt
    21361 gtattgctga aacaccatgc caacgttacg acgcaaatcg cgaatcgctt tgtcagaggg
    21421 tgttttggtg aaatcgaaat ggttgcctgc aatgttgagc gtaccggagc gcggcatctc
    21481 aagcagattg agtacacgca gcagcgagct tttacccgcg ccgctggggc caagtaacac
    21541 cagcgtttcg ccctgtgggc aatccagcgt gatatcgaac agcgcctgat gcgcgccgta
    21601 gaagcaatta atgccgttta attgaatact cattgacact cgtatactgg cagtctgata
    21661 gctattgagg tcgaagatag tacctttgac agaataatta tgcaatattt atgctttaaa
    21721 agttaaaagc aaagcgcatt attcaataaa catagcacaa aataacgggg gcggtggtcg
    21781 gtgagcataa atgtcggcat tcctcacgaa atgccggata atttacgggg tttattggtt
    21841 gatcaaggcg ttagcgattc tcgatggact gacggagcgt acccgccgtg gcatgaacgc
    21901 taccgcctaa gtagcgcaca tcgtcgatga cccaacactg gccttcctga atcattaaca
    21961 cttcatcctg ccagccctgg tcaccctgtt tgagatccac ccgcaatgga atgttacggg
    22021 catcacgatt agggatagtc gatgcactgg caacgtgggc gctgtctggc aaggtggttc
    22081 gactggagaa cggatcgctg cttaacaatt cacgatgact attatcgcgg ctggcatcgc
    22141 taagttgggt tgccagtttg tcactcaaat aggggcgcag ggcggtaatg tcattgctgc
    22201 gatgttgaat gcggtagtcg tagaattgtt gagccacact gtcaggccct ccctggacac
    22261 aggaaccggt gcgggggccg ttatctttat aagctggcgt gacggtggta caggcactca
    22321 gcagcagtgc acatggaata agtagagata gtgaacggta gcgcataatc atttccttat
    22381 tagcttgctg tctgcgtcac actacgacca aacgctatca gattatttaa tacattgttt
    22441 ttaatacaat tctgaaacaa tctgaacacc tgattgggtt tgagtccatt gctggctacc
    22501 cactcaaggt tgctcgtagc tgacttactt aaattaccca aacaatagac agtgtaaacc
    22561 atttgtgagg ttgagcgtgt tatatggctg aaatgatcat ttaaggtaaa tatgatagtt
    22621 ctgaaatggt ggtataaatc ctcctgtaga ggttaagagc atctgcaaga agccagtttc
    22681 acatttataa cgcgtgataa atgaatgcgg ttattgtcta atcattacaa aatttttcac
    22741 atgcgataag agatcctgcg attaagatga ttgccaaaat tacgttgtat ttttgaaaat
    22801 gagcgaaatt tgatagtccc agctttgact ctcgataata ttatttatgc tacaattgtg
    22861 ggtaaagaat ttaagctaat gaatttgagt gaagaataaa ttaaattgct tcagtatttc
    22921 gttaattaat ataaatacaa aaggcgttgt gcttttataa ttataaagcg cgaggagttc
    22981 ggtggattat cgtttttctt gggtgatttt taatatgata aacaatatat ctgatcaagc
    23041 atcatcattt ccaggcacac aactaaatca aagtgataat ttcctggatt cactgaggga
    23101 atttttcgcg atattaaatt ccagtcgtaa gggtgagttg tctacatggg ataccatcta
    23161 cttgcatctg atattagcaa taaatgctga cagtgatctc ataaaaaatg atgtgttgct
    23221 cgcggaaaat attccatcag cgaattacca atttaataca ttttttagca atactttcga
    23281 gattgatgtt aaaaaatatc tgggtaaatc agaagataac gaagttgaaa taaaagcggg
    23341 taatgaaaga atctctattg gaatacgtaa tgtatcgaat ggaaaacttg aaagacaaca
    23401 gtttcttttc ccactggact atgagaataa attacaagaa caattagata agtattttac
    23461 gattgaatcg cacccattat tatacaggta taccattggt agtaagattg cgaatgtgat
    23521 ttttgaaaag ttatacagtc gtattgactt taacaaagaa caatatatat cctttataaa
    23581 ggacgcattt attcattttt atgattatag caggcgatat gctattagtg agaatatcga
    23641 taaagacgct gtaacaaaca atatcgcatt gatgagcact ttttatgaca gtgataatac
    23701 gtcaggtgag gttttaaata atgattttac agaagaagag tcatttgaaa ctgcgttaga
    23761 tgtagagcat gcaatagtgc ttggatttgc tgatgataat tttgaaacaa aacctgttca
    23821 ttatcaggat ttattaacac gatttagtgc ctttcaagat accgtgttta atctgtttcc
    23881 tgaaatgcac tcttcgcatt accacgatat ttgtagcgta agtgtggata tgacaaaggg
    23941 aacgcaatgc atgattcact tgatggtgaa tgaagaagtt ttcatgtctc tgcctgttcc
    24001 agttgccacg atggtacgtg aagatgcaag taatttagtc aatcttaaaa cactactcaa
    24061 tgatggttgt ttcattaaat atagtcattt taatgacgtg gcattaataa agcaaaacat
    24121 aagtaatctc tatttatcac acactgttgt gaatgaaagc atattgaaaa aatgttgttt
    24181 tgaaaacggc agtttaggtg atgttaaaat aacaaatagc aatgtaataa acagtgcatt
    24241 caaaaatatt tcgtttagat ctgttaaaat taataacgta aatactcatt ctttaaaatt
    24301 tattaactgc aattttttca atgttgatat gataagagtt aatctcagca aatgtctttt
    24361 tcatgaatgt agtatgcatg gtgtcaagat aaaaccctgg ttgcctgtaa aatggacaaa
    24421 agaattaata agtgattatt tgtatggctg tttactatct ctgtactcta tctgtgcaag
    24481 ggatatatat aatatgaacg ccgggaataa cgtgaaggtt gctgcggatg ctttccttga
    24541 aattattttc tctcttaaaa ataaatattg tattaaacta ttaagcgctc aggatcgggc
    24601 gtttatatac gagtttgcaa gaatgatctt cgcttatatt aatgacaaaa gcattgagat
    24661 cttattactt tcttgttttg cggcggctga ccaaaaagca atccagcggt atagacctca
    24721 gtcacaagat ggggaagact ttaggagtca tttacaatat aagttgcctc tttccgctca
    24781 ttaatgttgt tggtgcaaca taataagaca ggtaagcggt accctataat gaaaccaata
    24841 tcgggttatg caacggataa ttgtaaagac ttaagtgggt gtttttgaca caatgagatg
    24901 atttcctaaa ctgatcaaaa ccttcttccc aacatggcat atcagtccga taatgcttta
    24961 gagatcgaag gcttaacaaa caaggagatc gatcatgcaa ttttctacaa ccccaactct
    25021 ggaaggcctg accatcgttg aatattgcgg tgtggtgacc ggcgaagcga ttttaggtgc
    25081 caatattttc cgtgatttct ttgccggtat ccgcgatatc gttggcggac gttccggtgc
    25141 gtatgaaaaa gaactgcgta aagcacggga gatcgccttt gaggaattag gctcccaggc
    25201 gcgggcgctg ggggccgatg ccgtagttgg tattgatatc gactacgaaa cggtcgggca
    25261 aaacggcagt atgctgatgg ttagcgtcag cggtacggcg gtgaaaacgc gtcgatgaga
    25321 agagtcttct ggctggtcgc tgccgctctg ttactggcag ggtgtgcagg cgaaaaaggc
    25381 attgtcgaga aagagggata tcagcttgat acccgacacc aggcgcaggc ggcgtatccg
    25441 cgcattaaag tgctggtgat ccactacacc gcagatgatt ttgatagctc gctggcgaca
    25501 ctgactgata agcaggtcag ctcgcattat ctgatccctg cggtgccacc gcgatacaac
    25561 ggtaaaccgc gcatctggca actggtgccg gaacaagaac tggcctggca tgcggggatt
    25621 agcgcctggc gcggggcaac gcgccttaac gacacctcta ttggcattga gctggaaaac
    25681 cgtggctggc aaaaatcatc cggagtgaaa tattttgccc cgtttgaaca ggcacagatt
    25741 caggcgctta ttccgctggc gaaagatatt attgcccgtt atcacatcaa gccggaaaac
    25801 gtagtggcac atgcggatat cgcaccgcag cgcaaagacg atccggggcc attatttcct
    25861 tggcagcaac tggcgcagca ggggattggt gcctggccgg atgcgcagcg ggtgaacttt
    25921 taccttgccg ggcgcgcgcc gcacacaccg gtagataccg cgtcattgct ggagcttttg
    25981 gcgcgctacg gttatgacgt aaaacctgat atgacaccgc gcgagcagcg gcgcgtgatt
    26041 atggcattcc agatgcattt ccgcccgacg ttatataacg gcgaagcgga tgcagaaact
    26101 caggcgattg ccgaagcatt gctggagaaa tacgggcagg attagcgcgg cagttttccg
    26161 tggtcgcgta gccaggcggc agttttctcg atgccttcat ccagggtgat gaccggctga
    26221 taacctaact cttcctgcgc acgcgtaata tccagcgtaa agtcaaaatt caacttggag
    26281 acgccgtagt gggtcagcgg cggctctttt gctgacttgc ggcctaaacg ctccatgctg
    26341 cgggcgatca tatccagcat cgggtaaggg acggaacgaa tacgacagtc aatattcaac
    26401 tcgtcgatca gcttctgcac gatgctgcgc agtgtgcgat gctcgccgtt ggtgatgttg
    26461 tacacacgcc cggaaggtag cttatcgcag gcttcctggc ttgccagcca cattgcgtgc
    26521 acggcatttt catagtaggt catatcgacc agcgcactgc cgccatgcgg taacagaata
    26581 ctgccgtagt ggtgcatcat atgcgccaga cggggaataa agactttatc gtgcggtccg
    26641 aacagacttt gtgggcgcag aatagtaaag cgcgtttgtg gattcgcctg cgaaagcata
    26701 ttgatcactt cttcgctggc tgctttgctg cgggcaaact cgttggcgaa gcggtgaggg
    26761 cgaaaatctt ctttaatatt gcgatggtgg tgataatcga agtacaggga gggggaagag
    26821 atatgaataa agttacgcac accccaggcg acagcccatt cacccaggcg gcgagtggcg
    26881 cgaacgttag ccagatcgaa agcctgttgt gtcccccagg gtgaggtaaa gctggagcag
    26941 tgccacagcg tatcaatgcc cgcgagcatc actttagctt gtgatgaaac cagctcggtc
    27001 agatccgtcg gaacaaactc tgcgcccatt ttttccagca atttacccat tgcttcgttg
    27061 cgaccgctcg cacgcacgct gatgcctttc tggcataaaa actctaccgc gtttcgacct
    27121 aagccgctgg tggcgccggt aaccagtacc ttcatatcaa tccactgttg ttgagaaaat
    27181 gacgtgcgca ttcttccgtg atttccccca tgatgcaatg ggaaacatga aagaataacg
    27241 caggttttgt cgattaatct gtgctttgtt ctgccagtct ggcgatttgt tttgccattc
    27301 cgcgaaaaat aaacagatgc gcggggatca tcaataacca gtaaaacagc cccggcatac
    27361 cgtgcggatg ccagaaagcg cggacatcga tagtacgata gtcaccttta tcttccaggg
    27421 taaaacacag tcgtcccagc cccggcgctt tcatgccaaa taacaacgta agttgctttt
    27481 tcggttcaac gacaatcact ttccagctat ccaccgcatc gccggtctgt aaatattcgc
    27541 gctccgggcg gcctttcgcc agattatgac cgatcgcgcg gtccatcaac gcccgtgtct
    27601 gccacaaaat attgccaaag aaataacgct ctttaccgcc gatttggttc accaccagcc
    27661 ataaagccgc aaggctggcg gacgttttaa cggtaaaccc cgcctgtttg gcgaaataac
    27721 cgtactccgg tcgccagcgg gcaaaggcct gagcgtcgta gccccagtcg ctggagttga
    27781 ccagtttttc ttcctctttc aacgtgctac gtaccgcgtc atcgaaagcg atcagccgtt
    27841 gtgggatgag tgcacgtagc gcggtatcat ctgccagcag atcgtgtttc agcccctgaa
    27901 tcaacgccct ggcggtggtg ggcggtacgg aagtaatcac attgagaaac cacaccgaaa
    27961 tccagcgggt ggggaggggg atggggatca accagcggcg cttaccgctc accgccataa
    28021 aatgttcaaa ctgttgctga taactgagca cctctggtcc ggcggcttcg aagatgcggt
    28081 gttcgttggc tggatggtct aacagcgcca ccagatagtg cagcaagttt tccagcgcga
    28141 tgggcgtggt gcgtgaacgt acccagcgtg gcggcgttaa cactggcagg ttgtagacca
    28201 tatcgcgcat gacttcgaac gccgctgaac ctgcgccaac gataattccg gcccgaagtt
    28261 cggtcactgg tacattcgct tcacgaagaa tgtccgccgt agcctgacga gctcgcagat
    28321 gatccgactg ctcatgtggc ggggcctgca acgaactgag aaagattaat tgcttaactg
    28381 gtacttcacg tagcgcatcg cggacgttga gagccagctg gcgctcctga gcgataaaat
    28441 cgccgccttc gcccatgctg tgcaccagaa aatagacggt atcgatatcc tgcaacaggg
    28501 ccggaaggtt atccggccag ctgagatcga ctttatggca actgacgttt gccagttgca
    28561 gctttgcaag cctgtcgaca tgacgtgccg ccgctaggat ctgatgccct tgctggctga
    28621 gtgtgcgcac cagatgctga ccaatgtagc cactggcacc gagaactaaa atgcgttgcg
    28681 gcacgtctct ctccttaacg cgccaggaat gcgcgccagt gggcggcgac ttccgccagt
    28741 tgttcgcgcg agacgtcaag atgcgtcacc aggcggacaa tcggcgaggc gttaatcagc
    28801 acgtttctcg ctttcatgta ttcgcctaac gcggcagcat tttcttcccc gacgcgaaca
    28861 aacagcatat tagtgtcctg acgcatcaca tccgcgcctg cttcacgcag ttgctccgcc
    28921 atccaggcgg cgttgtcgtg atcttcctgc aaccgtgcga cgttattttt cagggcatac
    28981 atcccggcgg cagccagaat cccggactga cgcatcccgc caccggccat tttccgccag
    29041 cggatggcac gtttaatgta atcacgatta ccgacgagta atgaaccgac tggcgtccca
    29101 agacctttcg acaggcaaat ggtgaacgaa tcacaatatt gcgtgatctc tttcagttcg
    29161 cagccgtaag ccaccacggc attaaagatg cgcgcaccgt caacatgcag cgccagattg
    29221 cgctcgcggg taaattccca tgcttctttc aggtattcac gcggcagcac tttgccgttg
    29281 tgggtgtttt ccagactgag taatttggtg cgggcgaaat ggatatcgtc gggtttgatt
    29341 ttcatcgcca ctttatccag cggtagcgtg ccgtcggcag ccgcgtcgat gggttgcggc
    29401 tgaatactgc ccagcaccgc tgcgccaccg gcttcaaaca gatagttatg cgcggcctga
    29461 ccgacaatat actcttcgcc acgttcgcag tgactgagca gagcgaccag gttggcctga
    29521 gtgccggtag gcagaaaaat ggcggcttct ttaccggaaa gctctgctgc atagtcctgc
    29581 agagcattaa cggtagggtc gtctccgtaa acgtcgtccc caaccggggc ggccatcatc
    29641 gcttcgagca tggcgcggct cggtcgggta acggtatcac tgcgtaaatc aatcatggca
    29701 tgtccttatt atgacgggaa atgccaccct ttttacctta gccagttcgt tttcgccagt
    29761 tcgatcactt catcgccgcg tccgctgata attgcgcgca acatatacag gctaaaacct
    29821 ttggcctgtt cgagtttgat ctgcggtgga atggctaatt cttctttggc gaccaccaca
    29881 tccaccaaca ccggaccgtc gatagagaag gcgcgttgca gggcttcatc aacttcagag
    29941 gctttttcta cacggatacc cgtaatgccg cacgcttcgg caatgcgggc aaagttggtg
    30001 tcgtgcagct cagtaccgtc tgtcaggtaa cctccggcct tcatctccat cgccacaaag
    30061 cccagcacgc tgttattaaa gacgataatt ttcaccggca gtttcatctg cactactgag
    30121 aggaaatcgc ccatcaacat gctaaaaccg ccatcgccgc acatggcgac cacctgacgt
    30181 tccggctcgg ttgcctgcgc acccaacgcc tgcggcatgg cgttagccat cgaaccgtgg
    30241 ttaaacgaac ctaacaggcg acgcttgccg ttcattttca gataacgtgc cgcccatacc
    30301 gttggcgtgc cgacgtcaca ggtaaagatg gcgtcatcgg cggcaaaatg actaatttgc
    30361 tgcgccagat attgcgggtg aatggctttc tcgctcggtt tagctaaatc gtccagccct
    30421 ttgcgggcat cgcggtaatc ttccagcgct ttatccagaa acttgcgatc ggttttttct
    30481 tccaccagtg gcagcaatgc gcgcagggtt gatttgatat cgccgaccag cgccatatct
    30541 accttgctat gcgcgccgat gctggctgga ttaatatcaa tctgaataat tttggcatcg
    30601 gtcgggtaga acgcacggta ggggaactga gtaccgagta gcactaacgt gtcggcgttc
    30661 atcatggtat ggaaacctga cgagaatccg attaacccgg tcattccaac atcatacgga
    30721 ttatcgtatt cgacatgctc tttaccgcgt agggcatgaa caataggtgc tttaattttc
    30781 ccggcaaact caactaactc tttatgcgcc cccgcgcagc cgctgccaca catcagggcg
    30841 atattgctgg aataacgcag cagttgcgcc agtttgcgta actcttcttc ttccggcatc
    30901 acgactggct gtggcgcatg ataccagtgc gtggttgccc cttctggcgc aggttttaac
    30961 gccacgtcgc ccggtaatac aacaacggaa acgccacggt taagcaccgc tttgcgcata
    31021 gcaattgcca gcacttgtgg gatctgctcc gggctggaaa ccagctcgca atagtgacta
    31081 cattcgcgga atagctcttg tgggtgggtt tcctggaaat agccgctgcc aatttcgctg
    31141 gagggaatat gagcggcaat cgccagtacc ggaacgttat tgcggtggca atcgaatagg
    31201 ccgttgatca agtgcaggtt gcctgggccg cacgatccgg cacagaccgc cagttctcct
    31261 gaaagttgtg cttcagcgcc agcggcaaac gccgccactt cttcatggcg ggtggacatc
    31321 cactcgatgg tgcccatgcg attaagactg tcgctaagac cgttcagaga gtcgcccgtg
    31381 actccccaga tgcgtttcac gcctgccgat tcgagtgttt tggcgatata agctgcaacc
    31441 gtttgtttca tggttctcca tctcctgaat gtgataacgg taacaagttt agttcatctg
    31501 acggaggggg aagggatggg agagaaagga ggcactaacg gttaaatagc ccgatgaaag
    31561 gaatatcatc gggcataagg cgattatgcg agaaccaaat ccccctgcgg atggcaggag
    31621 caggccagta cgtaaccttc agcgatttcg gcgtcggtca gcgtcattgt actgctcacc
    31681 gtatattcac cggaaaccac ttttgtctta cagcagccgc aaacacccgc acggcaggca
    31741 gcgacaaccg gaacgttatt gctttccagc gcctccagta gcgtggtgcc aaccggggcg
    31801 taaaattctc gtgccggttg cagtttggtg aatttcagac cgctggtcgc cgcttctgct
    31861 actggggtga agaatttctc tttaaagaaa cgcgtcacgc cgagcgcttt cacttcctgc
    31921 tctacccaat ccatatacgg agccgggccg caggtcatca cggtacgtga agctaagtca
    31981 ggtacatctg ccagcagttc gcgagtgaga cgaccagcga taaagccttc ggtaacgtta
    32041 ttttctgcca ccagcgttac cggatagtta cgccactcat cggcgaaaat aacatcctgc
    32101 ggcgtacgca cgttgtagat cacccgcaca tcggcctgtg gacggttctt cgcaagccag
    32161 cgacgcatcg acataatcgg cgtgacgccg cagcctgccg ccagcaacag gaatttatct
    32221 tctgctttat cgtcgcaggt aaattccccc atcgcgtccg aaagccagag ataatcaccg
    32281 cgttttacat cgcgcgtcag ccactgggag ccgacaccgt catcaatccg ccgcacggtc
    32341 agggtgatat attcactcac gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttcc
    32401 gctgagtttc gcacgctgac cagtgcatat tgcccggcgc gatatgggta gtgatcgtgg
    32461 caaatcaggg aaatcgtcca cacatccggc gtttcttgcg taatgtgatg aacctgtatc
    32521 cgccacgggc attgattcgt tggcatcgtc atcgacaaac tccttacgcg ttcaacagtt
    32581 gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc gaatttctcg ttcagcaccg
    32641 ccagcaggtc tggtgtcagg aaaccaggtg cagtcgggcc ggtgacgata tttttcacgc
    32701 ccagagaaag cagcgtcagc agaatgacaa tcgctttctg ttcaaaccag gagagcacca
    32761 gcgacagcgg cagatcgttc acaccacagc ccagtttctc tgccagagtg acagccagaa
    32821 taattgctga gtaagcatcg ttacattgac ctgcatctac cagacgcggc agaccttcga
    32881 tatcgccaaa ctcaagtttg ttaaagcgat atttaccaca ggcgagggtc aggatcaggc
    32941 agtcatccgg cacgctggtg gcgaaatcgg tgaagtagtg gcgctcgccg cgtgcgccgt
    33001 cacagccacc aagcaggaag atatgacgca gtttttcacg gctaaccaga tcaatcagcg
    33061 tatcggctgc accaagcagc gtctggcgac caaagcccac ggtgatcagg tgaggaattt
    33121 cgctgtacgg gaagcccgcc atttgttgcg cctgggcgat gaccgcagag aaatcttcgc
    33181 catccagatg acgcacgcca ggccagccaa cgatgctgcg ggtccagata cgatcgtcat
    33241 aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat cacgatgggg ccagggaaac
    33301 gagcgaactc cacttgctga ttctgccagc cgctgccgta gttaccgacc agatgcttga
    33361 atttacgcag ctccgggtag ccatgcgcag gcagcatttc gccgtgggtg tagacattaa
    33421 cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc tttgagatcg tgaccggaaa
    33481 tcagaatgca tttacccgcc gtcgctttga cgttgacctg ggttggcgtc gggtgaccgt
    33541 atttaccggt ttcgcctgca tccagaatgc tcatcacttt gaagttcatc tggccgattt
    33601 ccattgaaca ctcaagaagc gcgttcatat cggcaggcca ggtccccagc cacgccatga
    33661 ttttatggta ctgggcataa atatcgttgt cgtattgacc gagaacatgc gcgtgttcca
    33721 tataggccgc cgcacctttc aggccataca ggcacagcag acgcaggccg agaatgtttt
    33781 cgccaatcgc cgctttatct ttgttagggg taaattctgc tgcctgacgt tgcagctcgc
    33841 cgagatcatc gctcaccagt tgcaggtcag ccatcgggtt atcgacgcgc gcgttggcat
    33901 ctacagccag gcattgtgct ttcagcgcct cgcgcaaggc aatcgcttca cgagcgtagc
    33961 cgacaatacg cggagaatcg aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg
    34021 cgaagctgtc aacatcgtgg ttgatgatgc cgtattcacg cgcttttacc gcccaggcag
    34081 aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc agaagtttcc gccgttttac
    34141 cacacatccc ctgcgcgtat gagcagccgt tttctgccgg agtacggata gtttgttcac
    34201 attgcacaca aaacatgatc acacctttta aagttatatt taatatacat gtttaagggt
    34261 aagacgctta acgcggggat aaaagggatt tttcatgcaa ctttaaggga gattgattta
    34321 gcgcaatttt ggcggcaggg aactaccgcc agagaggtat tacgcagaga aaaaggcgat
    34381 gaggatcggc actaacaggc taagaataaa accgtgaaca attgccgccg ggaccatatc
    34441 cagcccgcca gtacgttgaa gaacgggcag ggtgaaatcc attgatgtgg caccgcataa
    34501 gcccagtgca gtagagcggc tgcggcgaat cagcccaggg atcaacataa tagcaatcag
    34561 ttcacgggcc agatcattaa aaaacgccgc gctcccgatt accggaccaa aagattcggt
    34621 caataaaata ccggaaagag aataccagcc gaaaccggag gccattgcca gcgcggtatt
    34681 gatggggaga tcaagaataa aggcgttaat taaaccacca attaatgaac tgacaaccac
    34741 caccacggcg acaatcattc cccggcgatt aaggacaatc tgctttaagg tcatgccatt
    34801 attgcgcaac tgaataccaa cgaggaaaag tagcaaaatt aacgtgtatt cactggcttc
    34861 ggtcgcgtgt tgtaagaaag ccagtccact cagaccaatg gcaaaaccaa tcactactac
    34921 gccgcacagt tttagcgact ccagcgccat cgcaatacgc gacgggagtt tttcttgctg
    34981 atggtggttg cgccacggca ggcctcgctc cagccacatc agggcggcaa tattacacag
    35041 taaaataacg gtaatactga cggcagaata atgcagaatc gccaacaggt tactggcgag
    35101 gttatcgaga aacgccagac tgatacccat aaaaaagaga ataaggtaaa ccatccagct
    35161 taatagctga ttaatagctt ttaacgcagc ttgttggcga agcggaatga ggtaacccac
    35221 aatcagggga accagaatga ttaacagccc agaaaacatg aaaatccagt ccttgcaaag
    35281 aagaagtcga aatgcgcgat gacacactac tgaaagcgga agggcgagta aagttgcaat
    35341 taaaaggaaa tgttatgcat cagaggggga tttttaatcc tatctgagtg tttagcattg
    35401 ttgcgctcct ttcatgtgca atgccatata aagccactgg cggagggagc gcgcaggggg
    35461 cagccagtcg ccgccgcccc ctgcaccccc gggctctggc gaacaaaatc gccgctgcgc
    35521 ggtaccttca gcttatcccc ttcggcaacc gggtcgggcg cgaggtaacg tcctgtaaga
    35581 cgcgccctca gcccacatcc atgtgggctg ccccggcctt cggggaacgc ttcagcgatt
    35641 ttgacgccac caatacccgt gcggtttgta atgtcgaaga taaaacaacc agggcagata
    35701 aaatgccaat gctaattaat cacgcttttc cagcagagtc cggtaaatca gaccaccgat
    35761 aatgccgccg acaattggca ccacccagaa gaaccacagt tgttctaatg cccagccgcc
    35821 ctggaagata gcaaccgcgg tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat
    35881 actgattaag tgaatcaggg ttaaggccag accaatagcg atcggcgcaa aacctgccgg
    35941 agcgaatttg tcggttgcac cgtggatcac taacaggaag cccgcactca ataccagttc
    36001 aactaccagc gcggaaagca tggaataacc gcctggtgaa tgctcgccat aaccgttaga
    36061 agcaaaaccg ctggctgccg cgtcaaaacc cgttttacca ctggcaatta aatacagcag
    36121 cgccgctgca acaataccgc cgacaacctg ggcaattacg tagccaacga cttcttttgc
    36181 cggaaaacgt ccgccagccc ataaaccaat agtgaccgcc gggttaaaat gaccaccaga
    36241 aatatgacca acagcgaagg ccatcgtcag aacggtcaaa ccaaatgcca gcgccacgcc
    36301 ggcaaaacca atgcctaatt ccgggaagcc tgcggccagt acagcactac cacagccacc
    36361 aaaaacaagc cagaaagtac caaaacattc agctgctaat tttctgaaca tatccacctc
    36421 aattaaaaat tgaccctgtg aaaa
//
LOCUS       DAJVRP010000032        34985 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVRP010000032 DAJVRP010000000
VERSION     DAJVRP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.032"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..815)
                     /locus_tag="OQN12_001642"
     CDS             complement(<1..815)
                     /locus_tag="OQN12_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1654013.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OQN12_001643"
                     /pseudo
     CDS             907..993
                     /locus_tag="OQN12_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1398..1874)
                     /locus_tag="OQN12_001644"
     CDS             complement(1398..1874)
                     /locus_tag="OQN12_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1654014.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OQN12_001645"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="OQN12_001645"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1654015.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="OQN12_001646"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="OQN12_001646"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1654016.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="OQN12_001647"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="OQN12_001647"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1654017.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="OQN12_001648"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="OQN12_001648"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1654018.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="OQN12_001649"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="OQN12_001649"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1654019.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="OQN12_001650"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="OQN12_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1654020.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OQN12_001651"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="OQN12_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1654021.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="OQN12_001652"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="OQN12_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1654022.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="OQN12_001653"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="OQN12_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1654023.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="OQN12_001654"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="OQN12_001654"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1654024.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="OQN12_001655"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="OQN12_001655"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1654025.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="OQN12_001656"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="OQN12_001656"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1654026.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="OQN12_001657"
     CDS             13849..14553
                     /locus_tag="OQN12_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1654027.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="OQN12_001658"
     CDS             14758..15471
                     /locus_tag="OQN12_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1654028.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="OQN12_001659"
     CDS             complement(15507..16463)
                     /locus_tag="OQN12_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCS1654029.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OQN12_001660"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="OQN12_001660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1654030.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="OQN12_001661"
     CDS             complement(17701..18462)
                     /locus_tag="OQN12_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1654031.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="OQN12_001662"
     CDS             18595..19005
                     /locus_tag="OQN12_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1654032.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="OQN12_001663"
     CDS             complement(18967..20073)
                     /locus_tag="OQN12_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1654033.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="OQN12_001664"
     CDS             complement(20084..21217)
                     /locus_tag="OQN12_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1654034.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="OQN12_001665"
     CDS             complement(21210..22946)
                     /locus_tag="OQN12_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1654035.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OQN12_001666"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="OQN12_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1654036.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OQN12_001667"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="OQN12_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1654037.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26024
                     /gene="rhlE"
                     /locus_tag="OQN12_001668"
                     /pseudo
     CDS             24837..26024
                     /gene="rhlE"
                     /locus_tag="OQN12_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OQN12_001669"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="OQN12_001669"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1654038.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="OQN12_001670"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="OQN12_001670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1654039.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="OQN12_001671"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="OQN12_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1654040.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="OQN12_001672"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="OQN12_001672"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1654041.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31428..31688)
                     /locus_tag="OQN12_001673"
     CDS             complement(31428..31688)
                     /locus_tag="OQN12_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1654042.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OQN12_001674"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="OQN12_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1654043.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OQN12_001675"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="OQN12_001675"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1654044.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34985)
                     /locus_tag="OQN12_001676"
                     /pseudo
     CDS             complement(33012..34985)
                     /locus_tag="OQN12_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8294 a         8966 c         9453 g         8272 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga acccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctagcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttgccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aacaggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgaccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaat gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 attatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgcggt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aaccatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgcgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgtccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgt gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaa
//
LOCUS       DAJVRP010000033        34951 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVRP010000033 DAJVRP010000000
VERSION     DAJVRP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.033"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..2262
                     /locus_tag="OQN12_001677"
     CDS             <1..2262
                     /locus_tag="OQN12_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCS1654045.1"
                     /translation="DAKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGI
                     TENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGG
                     NFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFY
                     LNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKT
                     TSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAIS
                     ASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISA
                     TVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRA
                     IVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRY
                     SEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVS
                     DSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQ
                     DLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRE
                     LMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEING
                     SEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKL
                     RGNVQTAQREVAP"
     gene            2259..3347
                     /locus_tag="OQN12_001678"
     CDS             2259..3347
                     /locus_tag="OQN12_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS1654046.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3435..4733)
                     /gene="exuT"
                     /locus_tag="OQN12_001679"
     CDS             complement(3435..4733)
                     /gene="exuT"
                     /locus_tag="OQN12_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCS1654047.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5217..6629
                     /gene="uxaC"
                     /locus_tag="OQN12_001680"
     CDS             5217..6629
                     /gene="uxaC"
                     /locus_tag="OQN12_001680"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS1654048.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6644..8131
                     /gene="uxaA"
                     /locus_tag="OQN12_001681"
     CDS             6644..8131
                     /gene="uxaA"
                     /locus_tag="OQN12_001681"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCS1654049.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8214..8765
                     /locus_tag="OQN12_001682"
     CDS             8214..8765
                     /locus_tag="OQN12_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1654050.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8770..10014)
                     /gene="sstT"
                     /locus_tag="OQN12_001683"
     CDS             complement(8770..10014)
                     /gene="sstT"
                     /locus_tag="OQN12_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1654051.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10610..11575)
                     /gene="alx"
                     /locus_tag="OQN12_001684"
     CDS             complement(10610..11575)
                     /gene="alx"
                     /locus_tag="OQN12_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1654052.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11846..12844)
                     /locus_tag="OQN12_001685"
     CDS             complement(11846..12844)
                     /locus_tag="OQN12_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1654053.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12923..13615)
                     /locus_tag="OQN12_001686"
     CDS             complement(12923..13615)
                     /locus_tag="OQN12_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1654054.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13688..14191)
                     /locus_tag="OQN12_001687"
     CDS             complement(13688..14191)
                     /locus_tag="OQN12_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1654055.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14276..15412
                     /gene="rlmG"
                     /locus_tag="OQN12_001688"
     CDS             14276..15412
                     /gene="rlmG"
                     /locus_tag="OQN12_001688"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1654056.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15697..16011
                     /gene="higB"
                     /locus_tag="OQN12_001689"
     CDS             15697..16011
                     /gene="higB"
                     /locus_tag="OQN12_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1654057.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16008..16424
                     /gene="higA"
                     /locus_tag="OQN12_001690"
     CDS             16008..16424
                     /gene="higA"
                     /locus_tag="OQN12_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1654058.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16469..18487)
                     /gene="fadH"
                     /locus_tag="OQN12_001691"
     CDS             complement(16469..18487)
                     /gene="fadH"
                     /locus_tag="OQN12_001691"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1654059.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18713..21064)
                     /gene="ygjK"
                     /locus_tag="OQN12_001692"
     CDS             complement(18713..21064)
                     /gene="ygjK"
                     /locus_tag="OQN12_001692"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCS1654060.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(21081..21500)
                     /locus_tag="OQN12_001693"
     CDS             complement(21081..21500)
                     /locus_tag="OQN12_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654061.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21478..21831)
                     /locus_tag="OQN12_001694"
     CDS             complement(21478..21831)
                     /locus_tag="OQN12_001694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654062.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21832..22152)
                     /locus_tag="OQN12_001695"
     CDS             complement(21832..22152)
                     /locus_tag="OQN12_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654063.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22286..23719)
                     /locus_tag="OQN12_001696"
     CDS             complement(22286..23719)
                     /locus_tag="OQN12_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1654064.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23782..24231)
                     /locus_tag="OQN12_001697"
     CDS             complement(23782..24231)
                     /locus_tag="OQN12_001697"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1654065.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24228..27305)
                     /gene="ebgA"
                     /locus_tag="OQN12_001698"
     CDS             complement(24228..27305)
                     /gene="ebgA"
                     /locus_tag="OQN12_001698"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCS1654066.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27488..28471)
                     /gene="ebgR"
                     /locus_tag="OQN12_001699"
     CDS             complement(27488..28471)
                     /gene="ebgR"
                     /locus_tag="OQN12_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1654067.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28689..29020
                     /locus_tag="OQN12_001700"
                     /pseudo
     CDS             28689..29020
                     /locus_tag="OQN12_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29062..30441)
                     /gene="ygjG"
                     /locus_tag="OQN12_001701"
     CDS             complement(29062..30441)
                     /gene="ygjG"
                     /locus_tag="OQN12_001701"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1654068.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30859..32379
                     /gene="aer"
                     /locus_tag="OQN12_001702"
                     /pseudo
     CDS             30859..32379
                     /gene="aer"
                     /locus_tag="OQN12_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32533..33156)
                     /gene="nfeR"
                     /locus_tag="OQN12_001703"
                     /gene_synonym="yqjI"
     CDS             complement(32533..33156)
                     /gene="nfeR"
                     /locus_tag="OQN12_001703"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1654069.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33433..34178
                     /gene="nfeF"
                     /locus_tag="OQN12_001704"
                     /pseudo
     CDS             33433..34178
                     /gene="nfeF"
                     /locus_tag="OQN12_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34232..34307)
                     /locus_tag="OQN12_001705"
     tRNA            complement(34232..34307)
                     /locus_tag="OQN12_001705"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34272..34274),aa:Ile,seq:cat)
     gene            34432..34938
                     /gene="mug"
                     /locus_tag="OQN12_001706"
                     /pseudo
     CDS             34432..34938
                     /gene="mug"
                     /locus_tag="OQN12_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8541 a         8978 c         9181 g         8251 t
ORIGIN      
        1 gatgcgaagt cattatattt aaatccagac aaaaatactg ttaacgcttt tatacatcag
       61 caagacatca atgttctggc acaggatgat taccaatcgt tgtctattca gggaaacggt
      121 gcgctgggaa taacagaaaa tagctatatt ggtgcacact ggaatttcaa cggttatgat
      181 gcagatgatg tcagtgacag taatactgac gtcagcgatc tctattatcg ttatgatttt
      241 ttacgtcgtt attatgtgca ggcgggtcgc atggacaacc gcacactatt taatgcacaa
      301 ggcgggaact ttacctttaa ctttctgcca ctcggtgcaa tcgatgggat gcgtatcggg
      361 tcgacactga gctatttaaa ccaggcgcaa agccagcaag gaaccccagt aatggttctg
      421 ctttcgcgca attctcgtgt tgacgcttat cgtaatgagc aacttttggg atcgttttat
      481 ctcaatagtg gttcgcaatt tattgatacc agttcctttc cgccaggcag ctacagcgtg
      541 gcattaaaag tctacgaaaa taaccaactc acccgcaccg agcttgtgcc gtttaccaaa
      601 accggcggtc tgactgacgg aaatgcgcaa tggttcttac aggcaggtaa aactacatca
      661 caggtttctg atgatgaaag ttcagcttat cagctaggag tacgcctgcc attacatccg
      721 caatatgagc tctacgcagg gctggcgaat gccgatgatg tgagtgcttt cgagttaggt
      781 aataactgga cggcagattt aggcagggtg gggaatcttg caatcagcgc cagcgtgttc
      841 cgtaacgatg acggcggcaa aggtgatatg caacaggcca actggagtaa tccgggatgg
      901 ccgacgttgg gcttttatcg gaccaactct gacggcgatg cttgtacaac cgacagcaga
      961 gagagctata acgccttaag ctgttatgaa agtatttccg cgacagtttc actgaatttt
     1021 gtcggctgga atatgatgct gggttatacc tgcacacaaa ataacactga tgatagtttg
     1081 cgttgggata aacagcagag ctttgaaaat aactatcttc gccagacaac agcgcaaagt
     1141 atctctgaaa ctgtacaact tagcgcttcc cgcgctattg tgatgcgtga ctggattttg
     1201 agtacgtcag tcggtgtttt ccatcgtaat gacaacggtg gcgataacga cgacaacggc
     1261 ttgtacttat cgttttcgtt atctgacacg ccaacgatgg atagcaataa caacagccat
     1321 tcaaccaatg tgcctacgga ttatcgttat agcgaacagg atggcgatca aacgtcatgg
     1381 cagttatcgc atacctttta taacgattca ttcagccata aagaacttgg cgtaaccgtc
     1441 ggaggcctga acaccgatac cataaacagc gcggttaacg ggcgttggga tgggcaatac
     1501 ggaaatgtct acgctaccgt atctgatagt tatgaccgta agaatcatga tcatctttcg
     1561 gcctttacgg gcacttatag ctctacgctg gctgtcagtc gctatggcgt taatttggga
     1621 gccagtggta cagacgattt gctgggtgcg gtactggtgg atgtgaaagg cttctctgaa
     1681 caggatgaag agagtcagga tctgcaactc gaagcgcggg tggcaggcag ccgaactttg
     1741 cagcttggtc aaagtgacag tgtgttgttc ccttatcctg gatttcagtc tggttttgtt
     1801 gaggttaacg acagtagcca gggcaatcag caggggacaa caaacatcat taacggtgca
     1861 gggaatcgtg aattaatgtt gttgcctggc aagctgcgct atcgcgaagt gtctgccagc
     1921 tttaactaca actatatcgg tcgtttatta ctgccggcag cggtgaaaaa attcccgatt
     1981 gtggggctga acagcgccat gttactggta gctgaagatg gcggatttac acttgaaatt
     2041 aacggcagcg aaaaagagct ctatctgctt tccgggcagc aattccttaa gtgtccactg
     2101 agtgttgtaa agaaacgcgc cagcattcgt tacagcggag atgttacttg tagtgtggtg
     2161 acttattcac aattaccgga gtcgattcag gttcaggcac agttgaaaca gcctaaatta
     2221 cgtggaaacg ttcagacggc gcaaagggag gttgcaccat gagaaatcga ttgattgcgg
     2281 cgatattggg cttgtttggc acgctcactg gcgttcaggc agctcctgac gtgaccagtg
     2341 aaattacgta tgatttggca tctggtagag cggattatta cttctggaag gatgaagcgt
     2401 cggcaggaaa taatggatat atgtggtatg aatgttcgta tcctgacctc caacaaacct
     2461 gtacagctaa tggaaatata tcgacagtac aaatctattt aactgaacaa cgcagtggga
     2521 tgcgttggcc ggtaaaactc aaaggattta aaacagccat tgtaagtagc gatgaagcgc
     2581 cgctaggatg caaggggggc aaagggcttc agacgaatct taaggattct aatagatctt
     2641 catgtacaga agatggtcaa cattattata tatacgatac aaagtttctt acgctctacc
     2701 ttgagcagac agagatgaag aatttgccga ttggtggcgt ctggaagggg aaagttaaat
     2761 tacattcgaa cagcccggcc caggactatt tcgcaaatat taccctgaat acgctcgacc
     2821 ccaaccatat tgacgtgttc ttcccggagt tcgcccacgc cacgccaagg gtgcagttag
     2881 acttgcatcc aacaggaagc gttaacggca gcaactacgc gcaagatctg accatgttgg
     2941 acatgtgcct gtacgatggt tttaacggta atgccatcag ttatgaaatc atgctcaaag
     3001 atgaagggcg accagctgca gggcgcagag acggttactt ctctatctat cgtcagggag
     3061 ggaccaccac cgacgaggga gaacgcattg attaccgggt caaaatgtac aacccggaaa
     3121 ccggtgggca aattgatgtg cgcaataatg aaaatatggt ctggaacagc attaacctga
     3181 aacgtgtgcg tccggtggta ctgcccggta ttcgctatgc cgtgatgtgt gtgccgacac
     3241 ccttgactct ggcagtcgat aaattcagcg tgatggataa acaggccggg tactacatgg
     3301 gcaaattgtc agtaatcttt acgccttcct tgccaaccat caattaaccc tgcaaaccgg
     3361 acaaacgctt ttttctatat aaacgacgaa gccgcctgat aaggcggctt cgtcgttata
     3421 acttacgtta aacgttaatg ttgcggtgcg ggatcgttat gtgtttcctg cgccacctca
     3481 atcgccggtt tgttctgcaa tacggtccag ataaccagcg cacccagcag gtcaaatact
     3541 gccagaactg cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc gacaaccagc
     3601 gcaaacagcg tacttgccag ccatgcggac atcccggtta aaccgttcgc cgtcgccact
     3661 tcgttacgac cgaacacgtc agaagagagc gtaatcagcg cgccagacag tgcctggtgg
     3721 gcaaaaccac cgatacacag cagcataatt gcgacatacg ggttggtgaa cagaccgatc
     3781 atacccgggc caatcatcag cacagcaccc agcgttacga ccatcttacg ggaaacgatc
     3841 aggttcacac caaaccaacg ctggaacagc ggcggcaggt aaccaccgag gatacaaccg
     3901 aggtcagcaa acagcatcgg catccaggcg aacatcgcga tctctttcag gttaaagccg
     3961 taaactttaa acatgaacag cgggatccac gcgttaaaag taccccaggc cggttctgca
     4021 aggaaacgcg gcagtgcgat accccagaac tgacggttac gcaggatctg accaacggac
     4081 attttcttcg ccgtgctcac ctggtgctgg gcttcctgac cattaataat atagtcgcgt
     4141 tcttcatcgg tcagatgctt ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc
     4201 gcccagataa agctcaatgc accggagatg ataaatgcca tctgccagct gtgcatcacg
     4261 attgcccata ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag
     4321 tagcctactg cgatggaacg ctctttcgcc gggaaccatt cggagctggc tttcagaccc
     4381 gccgggatca tcgctgcttc cgcggcaccg accgcaccac gagcaacagc taggccaccc
     4441 cagctacctg ccagcgcggt tgcaccacag aacacggccc acagcacagc aaacattgca
     4501 taaccgattt tcgtacccag cacatccagt acataacctg ctaccggttg catgaccgta
     4561 taagcagcag aataggctgc gatgatatag gaatactgtt gggtggagat gtttaactct
     4621 tccatcagag ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg
     4681 ccgagcgtca ccagtgcgat catataccaa cgtaaccctt taattttacg catgtaaaac
     4741 ctcatcgttt tgttatatcc gcaggaagcg cggccatcat cccgccagag gctgaagatg
     4801 ttagttacga ccgggtaacc gaaaaaaaaa ccggcacgcc ccgaacgttg ccatgaaagt
     4861 ttcgtgctaa ttcggcttcc gtaccggtta acccgattca tctaacaaat gaatgaatcg
     4921 gcaatttgca ttccgtcgac ttatgatttg cgacggcaga aagataactt gtcatacaac
     4981 cttaaaaggt gagagccatc acaaatgtgc gaatatttgt agggacatta cctgacgaca
     5041 gcgaggccag tactggcgcg gcctgcagcg agatttacca ctttgagagt aattttttta
     5101 actacgttta ttgatctaac tcaagaaaat atcttcggac tctggaaatt ggtgtgataa
     5161 ctttgtcagc atcgcaccat aagcaagcta gctcactcgt tgagaggaag acgaaaatga
     5221 ctccgtttat gactgaagat ttcctgttag ataccgaatt tgcccgccgt ctgtatcacg
     5281 actacgcaaa agaccagccg attttcgatt accattgcca tttgccgccg cagcagattg
     5341 cggaagacta tcgttttaaa aacctgtatg acatctggtt gaagggcgat cactacaaat
     5401 ggcgcgctat gcgtaccaac ggtgtggctg agcgtctgtg tactggtgat gcgtctgacc
     5461 gtgaaaaatt tgacgcctgg gcggcgactg ttccgcacac tatcggcaac ccgttatacc
     5521 actggacgca cctcgaactg cgtcgtccgt tcggtatcac tggcaaattg ctttctccgt
     5581 caactgccga tgaaatctgg aacgaatgta acgaactgct ggcgcaggat aacttctccg
     5641 cgcgcggcat catgcagcag atgaacgtga aaatggtcgg caccaccgat gacccgatcg
     5701 attctctgga gcatcacgca gagatcgcca aagacggctc tttcaccatc aaagtgctgc
     5761 cgagctggcg tccggacaaa gccttcaata tcgaacaggc gacctttaac gactacatgg
     5821 cgaagctggg cgaagtttcc gataccgaca ttcgccgctt tgctgacctg caaactgcct
     5881 tgaccaaacg tctggatcac ttcgccgctc acggctgtaa agtgtctgac catgcgctgg
     5941 acgtggtgat gtttgctgaa gcgaacgaag cggaactgga cagcattctg gcgcgccgtc
     6001 tggctggcga aaccctgagc gagcacgaag tggcacagtt caaaactgcg gtactggtgt
     6061 tccttggtgc cgaatatgca cgtcgcggct gggtacagca gtaccacatt ggcgcgctgc
     6121 gtaataacaa cctgcgtcag ttcaaactgc tggggccgga tgtaggcttt gactccatca
     6181 acgaccgtcc gatggcggaa gagttgtcta agctgctgag caagcagaac gaagaaaacc
     6241 tgctgccgaa aaccattctc tactgcctga acccgcgcga taacgaagtg ctgggcacca
     6301 tgatcggtaa cttccagggc gaaggtatgc cgggcaaaat gcagttcggt tccggctggt
     6361 ggtttaacga tcagaaagac ggtatggaac gtcagatgac ccaactggcg cagctcggtc
     6421 tgctgagccg ctttgtcggt atgctgactg acagccgtag cttcctgtca tacacccgtc
     6481 acgaatactt ccgccgcatt ctgtgccaga tgatcggtcg ctgggtggaa gcgggcgaag
     6541 caccggcgga catcaacctg ctgggcgaga tggtgaaaaa tatttgcttt aacaatgcgc
     6601 gtgactactt cgccattgaa ctgaactaag gtctggggtt gatatgcaat acatcaagat
     6661 ccatgcgctg gataacgtcg cggtcgcttt agcagatttg gctgaaggca cagaagtcag
     6721 tgtcgataac cagactgtta cgctgcgcca ggatgttgct cgtggacata aatttgcgtt
     6781 aacggatatc gcaaaagggg ccaacgtcat caaatatggc ctgccgattg gttatgcatt
     6841 ggcggatatt gcggcggggg aacacgtcca cgcccacaat acgcgcacga atctgagcga
     6901 tctggatcag tatcgctatc aacctgattt tcaggatctg cctgcgcaag cggcagatcc
     6961 tgaagtgcag atctatcgtc gcgctaacgg cgatgtcggg gtgcgtaacg agctgtggat
     7021 cctgccaacc gtgggctgtg tcaacggcat cgcgcggcaa atccagaacc gtttcctgaa
     7081 agagaccaac aacgccgaag gtaccgacgg cgtgttcctc ttcagccaca cctatggctg
     7141 ctcacaactg ggcgacgatc acatcaacac ccgcaccatg ctgcaaaaca tggtgcgcca
     7201 cccgaacgcg ggcgcagtgc tggtgattgg tctgggctgt gaaaacaacc aggttgccgc
     7261 attccgtgaa acgttgggcg atatcgatcc tgaacgcgtt catttcatga tctgccaaca
     7321 gcaggatgat gagatcgaag ccgggatcga gcatttgcat cagctgtata acgtgatgcg
     7381 caacgataaa cgcgagccag gcaaactcag cgaactgaag tttggtctgg agtgcggtgg
     7441 ttctgacggt ctttctggta ttactgctaa cccgatgctg gggcgtttct ctgactacgt
     7501 gattgctaac ggcggtacta ccgtactgac cgaagtgccg gagatgtttg gcgcagagca
     7561 gttgctgatg gaccattgcc gcgacgaagc aacgtttgaa aaactggtca ccatggtcaa
     7621 cgacttcaaa cagtacttta ttgcccatga tcagccgatc tacgaaaacc catcaccggg
     7681 gaacaaagcg ggcggtatca ccacgctgga agacaaatca cttggctgta cccagaaagc
     7741 gggttccagt gtcgtggttg acgtgctgcg ttacggcgag cgtctgaaaa cgccagggct
     7801 gaacttgtta agtgcgccgg gtaacgatgc cgtagcgacc agcgccctgg cgggtgcggg
     7861 ctgccatatg gtgctgttca gtactggtcg cggcacgcca tacggtggat ttgtgccgac
     7921 ggtgaaaatc gccaccaaca gtgaactggc ggcgaagaaa aaacactgga tcgactttga
     7981 cgcgggtcag ctgatccacg gtaaagcgat gccgcagttg ctggaagaat ttatcgacac
     8041 catcgttgag tttgccaacg gtaagcaaac ctgcaacgag cgtaacgact tccgcgaact
     8101 ggcgatcttc aaaagcggcg taacgctata aagtcgaaat gagttgttaa taaaaaacgg
     8161 cgtttcataa tgtgaagcgc cgtttgcttt tccatacctg ctaaggattt atcatgaccg
     8221 cgtattggct ggcccagggc gtgggtgtca tcgcctttct gattggtatc acaacatttt
     8281 tcaatcgtga cgaacgtcgc ttcaaaaagc agctttcggt ctatagcgcc gttattggcg
     8341 tacatttttt tcttctgggc acctatcccg ctggtgccag tgccatcctt aatgccattc
     8401 gtacattgat taccttacgc acgcgcagct tatgggtaat ggcgattttt attgtgctga
     8461 ctggcggaat tggcctcgcg aagttccatc atcctgtcga actattgccg gttatcggta
     8521 cgattgtcag tacctgggcg ctgttccgct gtaaagggct caccatgcgc tgcgtgatgt
     8581 ggttttcaac gtgttgctgg gtgattcaca acttctgggc ggggtcgata ggcggcacga
     8641 tgattgaggg tagttttctg cttatgaatg gcctgaatat cattcgtttc tggcggatgc
     8701 agaaaagggg aattgatccg tttaaagtag agaaaacccc tcccgccata gacgaaaggg
     8761 gttaaacaat taattacgca gggcgctatt tgccagacga tcgtcttccg cctggcaagc
     8821 tgccgcagtg aacagcacgt cagttgaaga gttcagcgcg gtttcgcaag agtcctgcaa
     8881 tacgccgata ataaagccga cggcaaccac ctgcatggcg atatcgttcg aaataccgaa
     8941 catattacag gccagtggga tcagcagcaa agaacccccc gccacgccgg atgcgccaca
     9001 ggcacacaga gaagccacta cgctcaacag cagcgctgtg ggcagatcga ccggaatacc
     9061 cagcgtatta accgcagcca gcgtcaacac ggtaatggtg attgctgcgc ccgccatatt
     9121 gatggtggct cccagcggaa tagaaactga ataggtatcg cgatccagat tcagcttttc
     9181 acacagcgcc atattcaccg gaatgttcgc tgcagagctg cgggtgaaga aggcatacac
     9241 accgctttcg cgcaggcaca gcagcaccag cgggaacggg ttacgacgaa ttttccacca
     9301 caccagcaat gggttaacca ccagcgccac cagtaacata cagccaacca gcacgaccag
     9361 cagttgcgcg tagccccaca gtgtggagaa accggtggtt gccagggtag aagaaaccag
     9421 cccaaaaata ccgaacggtg cgaagcgaat gaccagtttc accataaagg taacggcatt
     9481 cgacatatca ttgaccaggt ttttggtggt ctcgttaccg tgacgcagtg cgaagccgag
     9541 gccgatcgcc cacaccagaa tcccgatgta gttacctttc agcagggcgt cgatggggtt
     9601 ggaaaccatg ctcattacca gtccgcgcat cacttcgaca atgcctgacg gcggcgaaat
     9661 atcacccgcg ctactggaca agtgcagggt agaagggaag gcaaagctga agactactgc
     9721 ggccagagct gcagagaagg tgcccagcag atagaggaac aaaataggac ggatattggt
     9781 tttctgcccg tgctggtggt tagcaataga tgccattacc agcatcaaca ccaggatggg
     9841 ggcaacagct ttcagtgcgc cgacgaacaa agtacctaac agaccaacag cttccgccgc
     9901 gggttttgag atccatgcca gaagaatccc cagaacaagg ccgaccagga tttgttttac
     9961 caggctgcca tgagccagac gccggaatag ccccggtgaa cgttgcgtag tcatttttcg
    10021 atcctttcat tgtgttgtcg cacatccctg gtgcgttctg tcgacgcatc tttcaggatg
    10081 taacaaagtg tttgcaaggt cgagtataag gaaagttaca gcttcgggaa gcgaaaaatg
    10141 ctggatttta aagagaggtc atgttttgca acttaatggt tgcatttatt taacataaac
    10201 agagggaagg actggctact aaggctgatt agataacata tgggcaaaac tatttggaat
    10261 atgagaacgg ttgcgctcct ttaacgtgtt atgccatata aagtcactgg cgcagggagc
    10321 gcgcaggggg cggccaatcg ccgccgcccc ctgcaccccc aggctctggc gaacaaaatc
    10381 gccgctgcgc ggttccctca gcctatccct tgcggctacc gggtcgggcg caaggtaacg
    10441 tccctgtaag atgcgccctc gccccggcct ccagggaacg cttcggcgat tttgacgcca
    10501 ccagcacccg tgcggtcttt ttgtaaagag ctataagccg attacataat taatagctca
    10561 cctttgtctg gaatgagtta tctctaggaa tggcttaata tacagcgaat cattctcccc
    10621 gctgcttatc atgccgataa ttcacccagg cgttgataat aaacgtcatc accagaatgc
    10681 caaacaccac gcccagcgag acggcgattg gaatatggta gaagtcgaca atcagcatct
    10741 tgataccgat aaacaccaga atcaccgcca ggccatattt gagcatcgag aaacgctctg
    10801 ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag gattgcaaac aggtttgagg
    10861 tcagcacaat gaacgggtca gtggtcacgg cgaagatagc cggaatgcta tccacggcaa
    10921 aaatcacgtc gctcaattcc accagaatca gtaccagcat cagcggtgtg gcgtacagca
    10981 atccattctt acgcacaaag aaatgctcgt tgtcgatggt gtcggtcatg cgcaaatgac
    11041 cgcgtagcca gcgcaccagc ggcttgtcgc caatgcctga ttcatcttca tgggcgagcg
    11101 ccatcttcac gccggtaaac agcaggaagg caccgaagat atacaggatc cagtcgaact
    11161 gcgaaatcag ccagctgcct gtgaagatca tgatggtacg cagaacaatc gccccgagca
    11221 cgccatacac cagcacgcga cgttgtaatg ccgccggaac ggagaaatag ctgaacaaca
    11281 tcagccagac aaagacgtta tcgaccgcca gcgatttctc aatcagataa cccgtgagaa
    11341 aggccagggc ctgtggatcg gcaacggcgc gaccttcggt ttgcaccaga taccaccaga
    11401 aagcggcgtt aaacagtaac gacagcgtca cccagaccag cgaccaggcc gcagcctgtt
    11461 tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa caggtcgata gccagcataa
    11521 tggcgacaac aacagcgaat ccgccccata gcaacggcgt gccgacagta ttcatagaag
    11581 ttccttacac ataaaaaaac ggccaacgtc ggaagacgtc agccgctgct ttttatgcat
    11641 agacctcgcc ttccggcaag gtctcactta caacaaaaaa ggaatacgtc tgcgtattcc
    11701 tttcgggttg cccggtgacc ggatgtggtt tttcacacat cgtaatgacg atcgaccggc
    11761 aatgaagtta ctcccctttg caggtaacaa agtatgacag accattcagt cagtcaatgg
    11821 tctgttacat accttttaca ctgttttact ttggcaagta tagttttacg ctatctgccg
    11881 gaaaaatcac cccagtttgg cggcggatct cggtcagcag tttggctgtg atgcgactga
    11941 ccgccagccc tggatgatcc accagatgct catccaccag ggtagcgaac agctctgctt
    12001 cgtagagcat ggtattaata tgctgcggct gggtgagatc ctgcatctgg ctgccacgcg
    12061 gcacaaagca gactttctgg cattcagaca gtttttcaat caccagcgat cccgcttcgc
    12121 cctgaatctc gcttcccagg acagaatcac tgacctttga gtgctgcaag gtgacgctga
    12181 aatcaccgta atccatcacc accacgccgt gggcgtcaac gccgcttgcc agcaaactcg
    12241 ccgttgcctg cacgcttttc ggctcaccaa ataacgccac cgccgacgcc aggcagtaaa
    12301 agccgatatc cataattgaa ccgttagaga atgccggatt aaaggtgttg ggattctcac
    12361 cgtccaggta acgttgatac cgcgaggaat actggcaata gttgaaaaag actttacgca
    12421 gtttgcctgc tttcggcagc gcctggcgca acaaatgaaa gttcggcagg caggcggttt
    12481 taaatgcctc aaacagcacc acctgatttt cgcgcgcaca ggcaatggcg gcatccactt
    12541 ccgccagatt cgacgccagc ggtttttcgc aaatcacatg aattttatgg ctaaggaaaa
    12601 gttgtgtctg ggaaaagtgc agggaattcg ggctggcaat atacaccgcg tcaatggcat
    12661 cgctttccgc catcgcttcc agcgacgtaa acagatgctc gacagaaaaa tcattggcga
    12721 agtgctgagc ctgctcaagg ctgcgggaat atacggcggt taacttgtat ttaccgctct
    12781 catgggcggc ctcgacgaac tggcgagtga tccagttcgt accaatcaca gcgaaacgta
    12841 tcataacgcg tggatactcc ataaagggat tctgtcgtca atgtagcacg cctttttcat
    12901 cttcgacgtg cgcagatacg cattattctt tcgccggaca gccatgctca ctaaacggcc
    12961 caatcatgac cgatggcccg agatatttcg gctggcgatg gaggattttt gcgtcaatca
    13021 ccgcgctgac acgagcgagt ttctcccgca gttgagtata aaaaccgtgc ttcatattga
    13081 ggtctggcgc gttaaacccg atagcgtcga tgccgtactg ttttgccagc cagatggcgc
    13141 gttggttatg aaattcttgc gagataatgg tgatatggtt ttccccaaac acctttttgg
    13201 cacgcactac cgaatccagc gttgagaatc cggcatagtc gcaaaagatc actttcgccg
    13261 gtacgccttt agcgatcaaa gcctgctgca tacctgatgc ttcatcgtaa tttttacgtc
    13321 cgttatcgcc actcaccagt agccatttca cttttccggc gtggtacaac gctgctgcgg
    13381 tatcaatacg tcgggtaaag tagcgattac ccggcctcgc ccccagcaat aaacccacgt
    13441 tacgcgccgg gacggcgttg acatcgcccc aggtcagctg cttgctggcg ttgaccataa
    13501 cgcgatcggc gataaaaatg gtcgctcctg ccacaagtaa cagtaagcag gcaatcttta
    13561 gagtccggcg agaaaaacaa atacggagaa gaaggcgggc aaatgcgcgc agcatggtcg
    13621 ttatccttaa cgaaaggggg gaggtcgatt ttaggcgcta aagaaaagat aacgcctgac
    13681 atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc cgggtgtcga
    13741 actcaagctg gtggtactgc ggctccatat ggcagcacaa ttgatagaac gctttgttat
    13801 gctctttctc cttcaggtgc gccagctcgt gcaccacgat catgcgcaaa aacggttccg
    13861 gcgcgttgcg aaacacggta gcgacgcgga tctctgcttt tgctttcagc ttgccgccct
    13921 gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg atcttattgt
    13981 catacatcac tttattgatc ggcggggcat tacgcagaaa ctgatttttc agatcctgag
    14041 tatattgcca gagagcttta tcggtggcgt agtcgtgtgt tcccggatag cgttttgcca
    14101 gcacatcgcc cagacgctgt tcgttaatca gtgttcgcac ctgcgaaagc agctgctcgg
    14161 gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag aaaaagggta
    14221 tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag ggccgatgag
    14281 ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga cggatgacgt
    14341 taacccgcta caggcgtggg aagcggcaga tgaatatttg ctgcaacagt tggacgacac
    14401 agaaatcccc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa gctgcgcgct
    14461 ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg cgacgcgcga
    14521 gaatttacgc ctaaacggga ttgatgagtc gagcgtgaag tttctcgaca gcaccgccga
    14581 ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg cattgctgga
    14641 acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta ttgccggtgc
    14701 caaagcccgt gacattcaca cttccacgct ggaactgttc gaaaaagtgc tcggcccgac
    14761 caccaccacg ctggcatgga aaaaagcgcg cctgattaat tgcacgttca atgaaccgcc
    14821 gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact ggactatcca
    14881 taaccatgcg aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct tctttatgca
    14941 acatctgcca gagaatctcg aaggggagat tgtcgatctc ggttgcggta atggcgttat
    15001 tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg atgaatcgcc
    15061 gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg cgttggatcg
    15121 cagcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct ttaatgctgt
    15181 gctctgcaac cctccgtttc accaacaaca tgcgctgacc gataacgtcg cctgggagat
    15241 gttccactac gcccgccgct gcctgaaaat caacggcgag ctgtatatcg ttgccaaccg
    15301 ccatctggat tacttccata aactgaagaa aattttcggc aactgcacca ctattgcgac
    15361 gaataataaa tttgtggtgc tgaaagcggt gaagctggga cgtcgtcggt aagattggga
    15421 gttacctgcc ggatgcggcg taaacgcctt atccggccta cgttcagcac gttaccccta
    15481 ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgctcagttc tggaatcgcg
    15541 cttccataaa acaaaaaatc cctgtcacca tcctctacat tctcttgttt agcgtttttc
    15601 tacgtttatt cttccgtcac acagataaat tcatccgttg ccaatctgtc aacggatgtt
    15661 atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac tcaaaaagca
    15721 ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc tctggggaac
    15781 acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt cccatctctg
    15841 gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa tgaattacgt
    15901 gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt tatgacgcat
    15961 aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg
    16021 ccgacatctt gcaagcagga gaaaagctaa ctgctgtggc accttttctg gcgggtattc
    16081 agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg ctcaacgatc
    16141 ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc
    16201 ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc
    16261 gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat
    16321 ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat
    16381 tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac gtgccggatg
    16441 cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt gttccctggg
    16501 caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt aaatgcacag
    16561 ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc cctgcgcaga
    16621 tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca tgcagcccgt
    16681 catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc gagagcagga
    16741 tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg ctggctttgc
    16801 gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc gggcttaagc
    16861 caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg gcgatattct
    16921 ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac
    16981 aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca tcgagataac
    17041 tcaatacctt cggatgatcg atcccgtcga taggcggaac acgcggcacg atcccactgg
    17101 cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga ttgagtttca
    17161 gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg cagcgtttcg taaaactcct
    17221 ctttgccggg gatctgtttg gcgatattaa actgcccgcc gatctcgctg tgcgcatcaa
    17281 acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc
    17341 cagcgccgac caccgccaga ttttttttct gcacggcggg aaggattggc attttggttt
    17401 cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag atctgatcga
    17461 ggcagccctg gttgcagcca atacaagtgt tgatctcatc ggctcgccct gattgcgctt
    17521 ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat cgatactata tcggcatcgc
    17581 cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg gttaccagcg
    17641 gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg cgcggcacgg
    17701 gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat tccggtgttg ataatggtcg
    17761 cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc ggcaaaagtg ccgccgcctt
    17821 cgaccaggtc gagcatcgac aaccggtaaa taataatgaa gtcgttgccg acgcgttcgc
    17881 gcaccgtacg cactacttct acggcaaatc gcatccggtt gcggtaatcg ccgccccact
    17941 ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcagagc
    18001 ccatcacttc gacgccatcg tatccggcct cccgcgccag ttgcgcgcag cgggcgaaat
    18061 cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg
    18121 gggcctgcaa tgcggacggt gcgaccagat gcggttggta gctgtagcgc ccggtatgca
    18181 aaatttgcag ggcgattttg ccgccttcct gatgtacggc ttcggtaatg gtgcgatggt
    18241 gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg cctgttaaat
    18301 ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa
    18361 acgcagccag ccgttcagca ccgtccgggt attcctccag cccggtgtgc attgaaccca
    18421 tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt
    18481 agctcataaa attgtccagt atgtaaaata attgttatgt agtcggatga gttctaattt
    18541 agccgtcgat ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga
    18601 atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc ttgggggatt
    18661 gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agccgttgca aattactgct
    18721 tacggaaaaa atcgttatac aacatataca aatgagcggc actccaggag aaatttggtg
    18781 cgccttgctg cgcgcctgtc agcgggttgt aattctcctg aattgggcca tcggcggtta
    18841 atcctttggc gtgcctgaag aacgtatccg ccagcttcag ggcatcatca cgataaccgt
    18901 aacgctccat ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccgcgccagt
    18961 agatatcagc gccaaaagcc ggattggtta acgccgccgt tcccagcgga acaaaggtgt
    19021 tgaactcttt aggatcgagc atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg
    19081 caccgttaaa cagcggcgac cagccttccg gccctttacc gcgctcaaca atcggtttgc
    19141 ccgcgcagcc gttcgccagt ggtttatctt caatacgtac gtcatagtag aactgggtag
    19201 tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc
    19261 gtttggcctc ttctggctta ccgaggatgg ttgccatctc cgccagataa tggttatcgc
    19321 tgtacatata gctggcctga tccaccgact cctgcaatag cgagtagccc agcaacgttc
    19381 cgtcctgact gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccattag
    19441 cgacatattt atccaactgt tctttgtcga taaaaccaaa gacggcggca tcatcacggc
    19501 ccgattccca cgacgcagcg acctgtgccg gaatttccag actgtcgtac tggcctttct
    19561 ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc ttctttatcg ccttttttca
    19621 ccgtaaacag catctcgccg ctctcggtgt tgtgggcttt gtcgcgggtc gcgccatatt
    19681 ccggcacccc gttgccgtta tgatcgcggt tacgtaacca ccagtcgtga taggccacca
    19741 gtttcgggta catctctgcc aaccaggctt tatcctgggt gacgttgtat acttccatca
    19801 ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac
    19861 cacgctcggg gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac
    19921 gcacgctgtc gccaggctgg atctgccagg agaagaccgc gcggatattc tctttggcga
    19981 tatccggatt gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac ggccaggtct
    20041 gattgccgga gaaccagcgt ccggtcaccg acggcgtgac ggtgttataa ttcaccgcac
    20101 cgcccggtga gcgccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct
    20161 gttccggcgt cgcatccgga ttggttaacc ctttcttcag gtattcttcc cagcgttgct
    20221 gcgaggcggt gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt
    20281 tgctaacttc ctgagcggtc agcagatggg aataggtggt gtagagcgtg gtcgaaccgt
    20341 tgatatgcgc cttactggta aagcgattac cgttgatttc agtctgcacc ggcagggatt
    20401 tatgcacctg atattccgat tcgccggagg tcagcagatc ccaggtggcg cgcactttgc
    20461 caaaggtgac tttcaggcca tcacgggtgg cgctgatttt gcgctgatag tcagggtatt
    20521 cgccagcaat ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca
    20581 gttcgccatc ccacaccaga tccagcggtt tattgctgat gattttagtt tccagtagtg
    20641 acgtgcgcgg cgtggcgaag cgcagagtca tttcgacctg cacatctttt gctgtcagtt
    20701 tttgcaccag cgcgccggga atactgtatg cctccagcgt gaagttgact ttcttgccgt
    20761 cttgccagac agtcaggcgg tcgaaattgc tggccataaa gttgatgtat tcttccgtca
    20821 gcagcgcaac gcccggaaag ccgcccatgg tgttcgggcc gtctggcaac agatgaccat
    20881 gccaggctcc gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct
    20941 tcatgtactg cggcgcacca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg
    21001 catgagcgga aaaacttatc agcagagcgc aggttactgg cgttaaaata gttttgattt
    21061 tcatgagatt acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg
    21121 ggatcgttag aggcattttt catgtagaag cggctatcaa aagccgtggc gaaacgtccg
    21181 ccgccatatt cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagcgtgg
    21241 tcagggcgat gtttggcgta ggtgtaagcg gtggagagat aaacgccttt taacgcttca
    21301 tacatggcgc tcaccatcat cccgctttca cttcccggta cgtccagccc gtcggcacgt
    21361 ccggtatggt gccaggcgcg agcggcaaag ttaggggtaa acaggcccgt gagatagagt
    21421 tgtcctgtgc cgtcggtaat ttccagacca ttcatccagg tgatgccttc ggcaaggtca
    21481 tactggatgt aaccgttcat ccattgccgg ataggtgtat ttgtcgtcgt agcgatcgta
    21541 cttgccaaaa tgtagccagg ctttgctttc gtcatgatgt ccggcaggtg tggcggtgac
    21601 gctataacga aagttgccgc ttaagttctg taccttcagc gcggtcataa tgtcgcgggt
    21661 attgggaatg acgtagccca aatcgggtgt gaagtcgccc caccattgca ggtcatccag
    21721 cgaggaatcc tgacgcagac tgagcatgat ttcggtgcca tcattggtac gataaccgcc
    21781 atagaaacgg ttaatcccac cttcgaaacc accccagcta tggtcaggca cctaggcatt
    21841 accgtcgtgg tcggcctgca cggtccagcc ttcgccgtaa atcaggccga accaggagcc
    21901 gtacttcatc tccaggccac cttcaatata ggtgccgtcg ctgtatttat gtttattctc
    21961 accctctaaa tccatatatc caccaagccc taattcgccg taaaaacgaa atgaaggaga
    22021 agatgtcgag gtaattgcgg gtgtttttgt gtcgtcatgt tctgccggac gtgcttcttc
    22081 tcctgccgca gaaaaggcgt agcaaaacgg cagagcaagt aatgctctgc atggtgcagt
    22141 gattaatttc attgtgttaa tccatgagct aaagaaaaag tacagccttt tatcgttgta
    22201 attataaaaa tataaaggca gaaactattt tccctctcct catccaggaa gagggaaaat
    22261 gcctgctcat tttttattag tctgcttaat caacattgct ggctggcgtc gcttcaatat
    22321 ggcgctcttc agccgttaat ccctttatat atttgctgta tttccaccac gcaaagccga
    22381 ggaagataac aataccgccg acgttataaa aaatgatggt cagaatattc gcgccagtcg
    22441 ggaatgtcga agcgacaaac cctacggcaa atatcgcaat cagcattgaa acaacgataa
    22501 taccggtgcg gcgggagccc atacggaaat cgcgtggcag gtgatctaat ttggcgcgta
    22561 aattcaggta agccagcatg atgaataacg gcggaagcat ggacgctgcg gcggtcatat
    22621 taataatggt attcatcaga tcctgcacgg tattggaacc gagcatcggg ataatcatca
    22681 gcgggatgac gatcagaaac tggatccatg ctgcgcgcgc cggaacgccg ttttcgttaa
    22741 gttcgacggt tttcttacca aagatgcctt ccgggatttc ggagaagaaa attttcaccg
    22801 gcgttgcggt ccacatcagc agggaaccga acattgcggt aaaggacacc agaccgacaa
    22861 agcgattcat caacgcttcc ggcagaccaa aatacgccgc catgccgtgg aatacctgca
    22921 ccgatccgcc ggtaaatttc aactctttgc tgctgacgaa gacgttaatc agcaccgagg
    22981 agacggaata cagtacgccg ataaaaatcc cggcgaggat gatcacttta acgaacgatt
    23041 tcgaaccgcc tttgacgtcg ttaacgtaca ccgcgacgga ctccgcgcca cctgcggcca
    23101 taaagatcca ggtggtaacg ccgaggaacg cccagttgaa gttcgggatc atcgcgtcaa
    23161 cggtgatcgg atcggcaggc tgtacgccgc caaccagtgc cgtacctgcc agtaaaatgt
    23221 aggagagcgt taacagcagc atcagcgttg aagtgacgga ggtaattggc cccagcattt
    23281 tcgccccgtt ggtggaaacc caggtggaga aggcgaacag caccatactg ataacggtgg
    23341 tggcaaccgg cgtcataata tattcgtagc cgaggaaggc atacgaagca taagcaataa
    23401 cgcgcggcaa cagcgaggtg aaaaagaaca gatttacgaa ccagtaagtg taggcagtaa
    23461 taaatgccca acgtccgccc agcgaacttt ttacccacgc gtagacaccg gcttctgagt
    23521 ttttatttaa cgaaacaaat tctgcgatga tcagacagaa gggaataaaa taaaaaatcg
    23581 tcgcgaggaa aaacatcggt gccgaggcca gtccaagctc aatattatta ttgataacgt
    23641 tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa gccgaatttg ccgattgtat
    23701 tacgtttggt atcagacata acacatctcc gaaaatgtgt agggtagcgg tggcggcaaa
    23761 ctccttttgc cgccgtagtt gttatttgtt atggaaataa ccatcttcga tggtgacttt
    23821 gagaaccact tttttgaccg cgttattgca gataaaccga tacgcttcat ggatatcgca
    23881 aataacgatt tgcccttcgt ggacctctac ggtttctccg cagcctttta aatattcacg
    23941 gtcagtttca tcacgataat attccactac ctgtaatgtc tctttcggcg catattcaat
    24001 tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag cgtcgatgcc cggtaaatag
    24061 cgcatcggtc gcggagtcat tttccacacg gtaggtcaat gaatcaccga tggagtgggc
    24121 gacgccaggc tgaatgttgt cgatattttc aatcgcttca acgcagcgtt gccacttctt
    24181 gccagaggcg taaatctggc ggaactgttc taagttatcg atgatcctca ttgcttattc
    24241 tcgctgtgca aattcgtgga aaagaaccct gcgccgaact catacgacgc caggctttgc
    24301 gcggtagctt ctccgccaga aaccggcagc aacgtaaagc cgtagctgaa gtcacggaac
    24361 cagacgcgcc aggagtccag cacctcgctg ccccaggagt tggagccgag gccaagtagc
    24421 tggtggtcga ggttcagggt gatgtcatca ctgcgctgca gctcgttaca gtgctgggca
    24481 gcgtggatgt tttcctgggt atagtgccag gcgctgaagt taattgggcg ctgcggaacc
    24541 accagcagac cgttgccgtg gcggttagtc agtgccgtcc agcggacatg ctgacggttg
    24601 ccgttgttct gcgggaaggg atagttctcg aacatggcat cgacggtgct gcgccagata
    24661 tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc ccggtccacg accgtaatac
    24721 gccacctgat cgtattcgcc gttaatcccc atggtgaaac cgatgcacgg aatgatgtgc
    24781 ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa cctggccatc ggcagcgatg
    24841 cgccagatgt aggtacagcg catcccgaag tcaaacaccg gcggtgcaat aactgtgcgg
    24901 ctgatgatca acacttcacc atcgctctgt tctacggcaa agtcgcgcag atgttcctgc
    24961 atgatctgca aatgattcgg ctgccacaac ccttcgtact cctgcttgtg gttgtcgatc
    25021 atcggcttga agaagttgat ctttggctcg cgggtcagca gcgactcgcc atttacctgg
    25081 caggatgtcg gtttgccact catttttgag aaggtgatcg cgaagttgta gccgcgaacg
    25141 gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg cattatttgg tgcgaaaggc
    25201 actggctgcg cggtgttttc cttcagcggg aactgataag aggcgataga atgcccggct
    25261 tcgctgtagc gggtgcggga atctttggtc accgtaatgt tgaggaacgc ttcgcgggcg
    25321 tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt tcggcgcaac gtcgcgcagt
    25381 ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca cctctgcgtg cagggtgtag
    25441 tcatcaagcg tggtaaacca cagtttattt tcgactttca actcgccgcg agtcagcgcg
    25501 tggattttta ccggcgcgat aacctgtttg tactctttca ggcccgatcc cggcgtctga
    25561 tcggaataga tcaaaccatc aagacagaag ttatagttgt tgggatagtc gccgccgaat
    25621 ttataccaga cattgccgtg gtcgtcctgt gcctggatcc cgtggtcgca ccactcccag
    25681 acataatgac cctgaatgca atcgtgctta tagaagacgt tctggtactc cgtcagcccg
    25741 cccggtccgt tccccatcgc atgagcatat tcacagatga tgcgcggctt cggatgcggg
    25801 tattcaccaa actcattcat cagcggcacg cgggtgtaca tggtggaaat aatatcgacc
    25861 acttcagcat cgcgatcttc ttcgtaatga accagtcgcg tgtcatccag cgctttcgcc
    25921 gcatggtaca tcgcgcggat gttacagcca tagccggatt cattgcccag cgaccagatg
    25981 atgatcgacg gatggttttt ctgtgcgtgg atatggcgaa caatgcgctc gacgtagact
    26041 ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc cgacattagc aaagccgtgc
    26101 gattcgacgt cggtttccgc catcacaaac aggccgtaga tatcacacag ttcgtaaaaa
    26161 cgcggatcgt tcgggtagtg agcggtacgc acggagttga tattgtgctg cttcatcaac
    26221 tggagatctt tctcgacgcg atccattcca acggcgcggc ctttgcgatg atcgttgtcg
    26281 tgacggttga cgccgtgcag catcacataa cggttattga tccagaacag accgtcgcgc
    26341 actttgatat cacggaagcc aacgcgttgt ggcaccactt ccagaacgtt gccgttggcg
    26401 tctttcagcg tcatgaccag atggtaaaga taaggggatt ctgctgacca ttgctgcggc
    26461 tgttcgacag taaaagcaaa gctggcgctg gtcagttttt caattgccaa atgatcaatg
    26521 gcgctgctgt gcaccacgcg ttcgccatca aacagggtat attccagcgt cgtgacgaca
    26581 ggggaggcgg cgagattttc cagcaccact tcgcaggaaa gcgtggcatc gcaataggct
    26641 tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg ttaggtgttt tccgaccaga
    26701 taaacatcgc ggaagatccc cgccgaccac cacatatcct ggtcttccac gtaggtagag
    26761 tccgcccact gcatcacgcg cacacacaac aggttgtcgc cggttttaac catcgcgctg
    26821 atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac ccacatactg accgttaacg
    26881 tagacttcaa aataggtttc gacgccgtca aatttaatca gcgtctgttt accctgccag
    26941 ccgtcgctga gggtgaaaat acgttgatag gcaccggttg ggttatcgct ggggacaaac
    27001 ggcacatcga tggggaacgg aaaaccttcg tcggtatatt gcagtttgcc gtgaccttcc
    27061 atttgccaca tggcggggac ggtaatatgc ccccagtcag ccattaactc agaggtgaag
    27121 gcttctggta cttgcagcgg atggtcaaaa aagtggaaat tccactgact gcttaagggc
    27181 agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt gcgcaacaga atcatatgaa
    27241 aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga gctgaatgtt ttcccagcga
    27301 ttcatagggc ctcccggtag agagaaatcg gaaccattca tggtgatggt agtttgtgta
    27361 attgctgggg ggttagtaaa atttttacta aaaagcagca ggtaaatgca tttactttaa
    27421 ctggatcacg aaaataaacg gcagcactgt ccggcaaaaa tgattgccgg acagtgtgta
    27481 aggggattta acgggtcgtg ccgcgcagtt ttaatttgct gggaacgaag actagcagcg
    27541 gcagcgcgcg accatcgcgg gctttttcat acaccaggtt aacgccctga cttcccatca
    27601 tctcggaatg gatgcgcacg gtggagagcg gcggaaaggt aaatcgcgcg gtggggatat
    27661 cgttaacgct gataagcgaa atatcttgtg ggatgttcag gccacgttca tgaattgccc
    27721 gcagtacgcc gatagcaatg gaatcggaag caacaaacag tgccttcgga tagtcttccc
    27781 gcgccagcat ttgttttgcc agttcataac ccgacgaact ggaaaaaccg ccgcgccaga
    27841 tatcctcttc gcgtaccact tgtttcagtc ggccatattc cgcaaaggcg acctcacgaa
    27901 tatccgcctt gccaggctca tcttcaccgc caataaaacc aatacgatta acgccctggt
    27961 tgatatagaa gtcgatgatt tctttactga tgcgtgccag atcgatatcc accgcatcgt
    28021 aaccgctgcc gggttcgtga aagtcgataa aacagatatt gtcggtcaac gcgctggcag
    28081 cggcgcgcag ggcgggcgtg ggtttgccga caattaaaat accggtgacg tttttaatgt
    28141 ctggtaatcc gttgtgttca taacagttgg tcagttcgat cgccagcttt tcgcactgag
    28201 tttcaatgcc gtggcggatc gccagatagt aaggatcgtt gatctccagc tcctgctggt
    28261 agctgtagat agccagaata tggtgttggt tgactgcgcc tgtctgtagt ttacgggcgc
    28321 tactggtctt gtactccagc ttttcggcga tctcgagaat gcgatgtttc gtctcttctt
    28381 tcacattcaa tgtcggatcg tcatttaaga ccctggatac tgtcgccagg gatacgccag
    28441 cttcgattgc gatgtctttt aatgttgcca tgtttacctt ttttactttc cctgatgtgc
    28501 gcccccattg tagaggaagt gcttatcggg tcacctgttt tcagtaaata aagcgcgttt
    28561 ctttgacgct gtaacggtaa cacagcggct taggaatgtg atcacgcctg cattctccat
    28621 ttttagccta caggattgag aaaaactttt ctatagttta gtaaaatttt atccgaaggg
    28681 aggaagcaat ggaaaccgtg gcttacgctg attttgcacg tctggaaatg cgcgtcggaa
    28741 agattgtgga agtgaaacgc catgaaaacg ccgacaagct gtacatcgta caggttgatg
    28801 tggggaaaaa acactgcaaa ccgtgaccag cctggtgccg tactacagcg aagaggagct
    28861 gatggggaaa acggtggtgg tcttatgcaa tctgcaaaaa gcgaaaatgc gtggtgaaac
    28921 gtcggaatgc atgctgttgt gtgcggaaac cgatgacggc agtgaaagcg tgttgttaac
    28981 gccggagcgg atgatgcctg cgggcgtacg cgtcgtataa aaagatcgga tggcgacgtc
    29041 gtatcgccat ccgatttgat attacgcttc ttcgacactt actcgcatgg ccgccagcgc
    29101 cttacgcgcc gctttgatca ccagttcaca ctgttcaatg gtcagtgtca gtggcggttc
    29161 aatgcggatc gttttggcgt tattgagcgt tccggccacc agtacgcgct ggcggaacat
    29221 ctcgctggca aagttatagc cgatttcgtt atcaacaaac tcaatcgcca tcaacatccc
    29281 tttaccacgc gcttcctgta ccagatcggg atattcccgc gccagttgac ggaaaccgtc
    29341 cagcaacata tcgccttttt gctcagcctg agccggtaag ttctgctcca gcaacacatt
    29401 gatggtcgcc agcgccgccg cacaggccag cgggttgccg ccaaaggtgg tggtatgcag
    29461 gaatgggttg tcgaacagaa ctgaaaacac ctcttcagtg gcgatggtcg cgccaatcgg
    29521 catcacgccg ccgccgaggg cttttgccag acacagaatg tccggctgaa cgttctcatg
    29581 ttcgcaggca aacatcttgc ccgtgcgccc catgcccgtt tgtacttcat cgaggatcat
    29641 cagtgcgccg aactcatcgc acagcttacg cacggcggtg agatagcccg gcggcggcag
    29701 aattacgcca ccttcgccct gaatcggttc gaggatcacc gcagccacat catcaccggt
    29761 ttttttgcac tcattaagag ccgtgcgcat ggcttcgata ttgccaaacg gcacatgacg
    29821 gaaccctggc agcagtggca taaacggttt gcggaaggtc gatttcgccg tggccgacag
    29881 cgcgccaagt gatttaccgt ggaacgcgcc gctggtggca ataaaagtaa atttgccgcg
    29941 cggcgactga taagccttcg ccagcttcag cgccgcttca acggactcgg tgccgctatt
    30001 acagaagaag ctgtatttca gtttaccggg cgttagcgca gcaagggttt tcgccagcat
    30061 agcccgtaac ggatcgagta gctcctggct gtgcagtggt tgtttcgcaa gttgattctg
    30121 tacggcggaa accacaactg gattacggtg ccccacgttg aaaattccaa aacctcccag
    30181 gcagtcgata aactcctgtc cctgggtgtc gacaagcgta tttaaacctc ccgcttgcca
    30241 ctctacggct ccgtaatccc cgccggcggt aacagatttg cgatactcta aaaaccccgg
    30301 attgacatgc tctttgaagt attcaatcac ctctcggtta agtgctttca tctcctcatg
    30361 atccagcgtt cgcttctcaa tgagattcag ggcgtgggcg ctgcacgcta aagccgatgc
    30421 gctcgaaggt aacctgttca aaatatgctc cggaggctcg cgtatcacat gataccgatt
    30481 taagtattgc agggattgcg ccactccggc tgcgatcgcg aaaatcggga taaacacaaa
    30541 tgcggttatc atttgtgcaa tatttaatgc tatccgttaa catttgttgt tattttgcac
    30601 ggtttttgtc gggtatggat ttgatgattg ctgcgcaaat gcacgttttc agggcaataa
    30661 ttgcctgatt tttgggcaag ataattaacg ttttcgtata gtaacagagt gaaagtattt
    30721 taaatcaaag aattagattt gtgctttaat cgcaaattgc gatctaaatc aaattaatcg
    30781 gttaaagata accgcagcgg ggccgacata aactctgaca agaagttaac aaccatataa
    30841 cctgcacagg acgcgaacat gtcttctcat ccgtatgtca cccagcaaaa taccccgctg
    30901 gcggacgata ccactctgat gtccactacc gatctgcaaa gctatatcac tcatgctaat
    30961 gacacttttg tgcaggtgag cggctttacc ttgcaagagt tacaagggca gccgcacaac
    31021 atggtgcgtc acccggatat gccaaaagcg gcgtttgcgg atatgtggtt caccctgaaa
    31081 aaaggggagc cctggagcgg catcgtgaaa aatcgccgca aaaatggcga ccattattgg
    31141 gtgcgggcca atgcggtacc gatggtgcgc gagggaaaaa tcagtggcta tatgtcgatt
    31201 cgtacccggg cgacggatga agagattgcg gcggtggagc cgctgtacaa agcgttgaac
    31261 tccggacgta ccagtaagcg tattcataaa ggcctggtgg tgcgtaaagg ctggctgggt
    31321 aaactgcctt cattaccgct tcgctggcgg acgcgtggag tgatgaccct gatgtttatc
    31381 ttgctggcgg ccatgctttg gtttgttgct gccccggtgg tgacgtattt cctctgtgtg
    31441 ttagtggtat tgttggcaag cgcttgtttt gaatggcaga ttgtgcgccc gatagaaaat
    31501 gtcgcccgtc aggcactgaa ggtggcgacc ggagagcgta atagtgttga gcacctgaat
    31561 cgcagcgatg agctggggct gacattacgc gcggtagggc agcttggcct gatgtgccgt
    31621 tggctaatta acgatgtctc aagccaggtg tccagtgtca gaaatggcag tgagacgctg
    31681 gcgaaaggca ccgatgaact gaacgaacat acccagcaga cagttgataa cgttcagcaa
    31741 acggtggcga ccatgaacca aatggcggcg tcggtgaaac agaactctgc cacggcgtcg
    31801 gctgccgata aactttctat caccgccagt aatgcggcag tgcagggtgg ggaggcgatg
    31861 accacggtga tcaagacaat ggacgatatc gccgacagta cctagcgcat tggcaccatt
    31921 acttcgctga ttaacgatat tgcgtttcag accaatattc tggccctgaa tgcggcggtg
    31981 gaagcggcgc gtgccggcga acagggcaaa ggttttgcag tggtggcagg ggaagtgcgt
    32041 catttagcca gccgcagcgc taatgctgcc aacgatattc gcaagctgat tgatgccagt
    32101 gctgataagg tgcaatccgg ttcgcagcag gtacacgccg ccggacggac gatggaagat
    32161 attgtggcac aggtgaaaaa cgtcacccag ttgatcgccc agattagcca ttcaacgctg
    32221 gaacaggccg atgggctttc cagcctgacc cgtgcagtgg atgagcttaa cctgatcacc
    32281 cagaaaaatg ccgagctggt ggaagagagt gcgcaggtgt cggcgatggt gaaacaccgc
    32341 gccagccgac tggaagacgc ggtgacggtg ctgcattaat cggtatgccg gatccggcgg
    32401 ttggagcaca atgcctgatg cgatgctggc gcatcttatc aggcctgcgg ggtgtgtagc
    32461 gggtgtaggc cggataaggc gcgtcaagcg tcgcatccgg caattgcacc gcgccactgg
    32521 cggatgcggc gattaatcca gttgcgtaat atcaaaagcg gcgcggtcga tcaccgcaat
    32581 gatctttttt atttgtgcat cactgatatc gctctggttg acgcgtaaat ccagcaccgc
    32641 tttaaaatta tccagcgccc gcttcatttg cgggttctgg cgcagcgccg cgccaacgca
    32701 acgcgctttg atgcgttctt caatcatctc cacttgttcg cggttttctt ccagccactg
    32761 cgcaccttgt tcggtcagcg caatctgctt cttaccacct tcctcttcgc ggatggtaat
    32821 cagcgactgc tcctgcagaa aatccagcgt cgggtagatg acgcccgggc ttggggtgta
    32881 attcccctgg gttagattct caatcgcttt aatcaattcg taaccgtggc tgtcatcgcg
    32941 cgagagaata tccagaatca ccagacgcaa ttcaccgtga ccaaagaaac gttgccgacg
    33001 accgccgccg cgaccgtggc gaccaccgca acattgaccg tgttcatgct ggtgaccgtg
    33061 tccgcagtgc tcgtgttctg acttctcacc tttgcagcag ccctcatgcc gtggctggcc
    33121 ttcatgttta caacaccctt cgtgatgatg gctcatatca gcctcctttt gcttgattag
    33181 atatatctaa tttatatctg atgtgagcat tactgcaagt acaaaaatga tttttaaacg
    33241 tggaagcacc tcacatctag gccggataag acgttcacgc cgcatccggc agcattgcta
    33301 tggcactgaa tgccttcaca tacttcagac atatcagttc tacaaatcgc ttgcatttat
    33361 catgattaac aatcattatc atttgcgaat tttatttaga tatatctgat taccatcacg
    33421 aaggcgataa caatgaataa caccccccgc tacccgcagc gcgttcgcaa tgatctgcgc
    33481 ttccgtgaac tgactgtgct acgcgttgag cgcatcagcg ccggttttca gcgcattgtc
    33541 cttggcggcg aggcgttgga cggttttaca tcgcgcggct ttgacgatca cagcaaactc
    33601 ttctttcctc aacctgacgc tcactttgtg ccgccaacgg taacggaaga gagcatcgtc
    33661 tggccggaag gaccacgccc accgtcgcgt gactatacgc cactgtatga cgaactacgc
    33721 catgaactgg cgatcgattt ctttattcac gacggtgggg tcgccagcgg ctgggcgata
    33781 aacttacggt ggcaggtccg cgcggttcgc tggtggtgcc ggaagattac gcgtatcagc
    33841 tgtatgtctg cgatgaatcc ggaatgcccg cactgcgccg ccgcctggaa acgttgagca
    33901 aacttgccgt taaaccgcaa gttagtgcgc tggttagcgt gcgggataac gcctgtcagg
    33961 attatctcgc gcaccttgat ggttttaata tcgaatggct ggcacatgat gagcaggcgg
    34021 tagatgcgcg tctggcgcag atgcaaatcc ctgccgatga ttacttcatc tggataaccg
    34081 gcgaaggtaa agtcgttaag aatttaagcc gccgctttga agcggaacag tatgacccac
    34141 agcgggtacg tgcagcggct tactggcacg caaagtaact gatatataaa aagaaaaagg
    34201 ctgacgattt ctcgtcagcc tttgctatat ctggtggccc ctgctggact tgaaccagcg
    34261 accaagcgat tatgagtcgc ctgctctaac cactgagcta aggggccatg gtagcggatt
    34321 ataaagtaac tccgtgtcgc aatccagcca ttaccgcgcg cctgctgttt ttataaacaa
    34381 tgtattttca atccgttata cttttcctgg tgatgttaga aaggagtaaa tatggttgag
    34441 gatattttgg ctccagggtt acgggtcgtg ttttgcggta tcaaccctgg gctttcatcc
    34501 gccgggactg gttttccctt tgctcatccg gcaaatcgct tctggaaggt gatatatcag
    34561 gccgggttta ccgaccgtca gttgaagccg caggaggcac agcatctgct ggattatcgt
    34621 tgtggcatca ccaaactggt agaccgtcca acggtgcaag ccaatgaagt ttcaaagcag
    34681 gagctgcacg caggtgggcg taagctgatt gaaaaaattg aagattatca gccgcaggcg
    34741 ttggcgattc tgggcaaaca agcatatgaa cagggattca gctagcgcgg tgcacagtgg
    34801 gggaaacaaa cgctcaccat tggttcgacg cagatttggg tgctgccaaa tcccagcggt
    34861 ttaagtcgcg tttcactgga gaaactggtt gaagcgtatc gcgagctgga ccaggcgctg
    34921 gtagtgcgtg ggcgataaaa aacgtcaccg g
//
LOCUS       DAJVRP010000034        34445 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVRP010000034 DAJVRP010000000
VERSION     DAJVRP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.034"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..332)
                     /locus_tag="OQN12_001707"
     CDS             complement(<1..332)
                     /locus_tag="OQN12_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654070.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="OQN12_001708"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="OQN12_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS1654071.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="OQN12_001709"
     CDS             complement(1693..2073)
                     /locus_tag="OQN12_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCS1654072.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="OQN12_001710"
     CDS             complement(2087..2503)
                     /locus_tag="OQN12_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654073.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OQN12_001711"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="OQN12_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1654074.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="OQN12_001712"
     CDS             complement(3504..3971)
                     /locus_tag="OQN12_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1654075.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="OQN12_001713"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="OQN12_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1654076.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="OQN12_001714"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="OQN12_001714"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1654077.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="OQN12_001715"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="OQN12_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS1654078.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OQN12_001716"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="OQN12_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1654079.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="OQN12_001717"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="OQN12_001717"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1654080.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="OQN12_001718"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="OQN12_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1654081.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="OQN12_001719"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="OQN12_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1654082.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OQN12_001720"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="OQN12_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1654083.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="OQN12_001721"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="OQN12_001721"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1654084.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="OQN12_001722"
     CDS             complement(14626..15441)
                     /locus_tag="OQN12_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1654085.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="OQN12_001723"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="OQN12_001723"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1654086.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="OQN12_001724"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="OQN12_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1654087.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="OQN12_001725"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="OQN12_001725"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1654088.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="OQN12_001726"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="OQN12_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1654089.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="OQN12_001727"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="OQN12_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1654090.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="OQN12_001728"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="OQN12_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1654091.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="OQN12_001729"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="OQN12_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1654092.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="OQN12_001730"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="OQN12_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1654093.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="OQN12_001731"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="OQN12_001731"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1654094.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OQN12_001732"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="OQN12_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1654095.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="OQN12_001733"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="OQN12_001733"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1654096.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="OQN12_001734"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="OQN12_001734"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1654097.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OQN12_001735"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="OQN12_001735"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1654098.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OQN12_001736"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="OQN12_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS1654099.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="OQN12_001737"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="OQN12_001737"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1654100.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="OQN12_001738"
     CDS             31575..32189
                     /locus_tag="OQN12_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1654101.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="OQN12_001739"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="OQN12_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1654102.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="OQN12_001740"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="OQN12_001740"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1654103.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8104 a         8702 c         9305 g         8334 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggta
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaaccc accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc aggccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaaca
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gtgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtga tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgccg ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt atgaacaccc
    34441 tgcgc
//
LOCUS       DAJVRP010000035        34088 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVRP010000035 DAJVRP010000000
VERSION     DAJVRP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.035"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..579
                     /locus_tag="OQN12_001741"
     CDS             <1..579
                     /locus_tag="OQN12_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1654104.1"
                     /translation="LERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYA
                     SQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDN
                     TQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWR
                     VQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(576..1712)
                     /locus_tag="OQN12_001742"
     CDS             complement(576..1712)
                     /locus_tag="OQN12_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1654105.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1718..2041)
                     /locus_tag="OQN12_001743"
     CDS             complement(1718..2041)
                     /locus_tag="OQN12_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1654106.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2224..2367
                     /locus_tag="OQN12_001744"
     CDS             2224..2367
                     /locus_tag="OQN12_001744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654107.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2586..4124)
                     /gene="aldB"
                     /locus_tag="OQN12_001745"
     CDS             complement(2586..4124)
                     /gene="aldB"
                     /locus_tag="OQN12_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCS1654108.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4289..5440)
                     /gene="yiaY"
                     /locus_tag="OQN12_001746"
     CDS             complement(4289..5440)
                     /gene="yiaY"
                     /locus_tag="OQN12_001746"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCS1654109.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5630..7474)
                     /gene="selB"
                     /locus_tag="OQN12_001747"
     CDS             complement(5630..7474)
                     /gene="selB"
                     /locus_tag="OQN12_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1654110.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7471..8862)
                     /gene="selA"
                     /locus_tag="OQN12_001748"
     CDS             complement(7471..8862)
                     /gene="selA"
                     /locus_tag="OQN12_001748"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1654111.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8960..9568)
                     /locus_tag="OQN12_001749"
     CDS             complement(8960..9568)
                     /locus_tag="OQN12_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1654112.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9639..10724)
                     /locus_tag="OQN12_001750"
     CDS             complement(9639..10724)
                     /locus_tag="OQN12_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1654113.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10727..11089)
                     /locus_tag="OQN12_001751"
     CDS             complement(10727..11089)
                     /locus_tag="OQN12_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1654114.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11626..13539
                     /gene="mtlA"
                     /locus_tag="OQN12_001752"
     CDS             11626..13539
                     /gene="mtlA"
                     /locus_tag="OQN12_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1654115.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13634..14782
                     /gene="mtlD"
                     /locus_tag="OQN12_001753"
     CDS             13634..14782
                     /gene="mtlD"
                     /locus_tag="OQN12_001753"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1654116.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14782..15369
                     /gene="mtlR"
                     /locus_tag="OQN12_001754"
     CDS             14782..15369
                     /gene="mtlR"
                     /locus_tag="OQN12_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1654117.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15381..15590)
                     /locus_tag="OQN12_001755"
     CDS             complement(15381..15590)
                     /locus_tag="OQN12_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654118.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15874..16236
                     /locus_tag="OQN12_001756"
     CDS             15874..16236
                     /locus_tag="OQN12_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1654119.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16780..17463
                     /locus_tag="OQN12_001757"
     CDS             16780..17463
                     /locus_tag="OQN12_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1654120.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            17507..21872
                     /locus_tag="OQN12_001758"
                     /pseudo
     CDS             17507..21872
                     /locus_tag="OQN12_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            22240..23895
                     /gene="lldP"
                     /locus_tag="OQN12_001759"
     CDS             22240..23895
                     /gene="lldP"
                     /locus_tag="OQN12_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1654121.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23895..24671
                     /gene="lldR"
                     /locus_tag="OQN12_001760"
     CDS             23895..24671
                     /gene="lldR"
                     /locus_tag="OQN12_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1654122.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24668..25858
                     /gene="lldD"
                     /locus_tag="OQN12_001761"
     CDS             24668..25858
                     /gene="lldD"
                     /locus_tag="OQN12_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1654123.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26054..26527
                     /gene="trmL"
                     /locus_tag="OQN12_001762"
     CDS             26054..26527
                     /gene="trmL"
                     /locus_tag="OQN12_001762"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1654124.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26580..27401)
                     /gene="cysE"
                     /locus_tag="OQN12_001763"
     CDS             complement(26580..27401)
                     /gene="cysE"
                     /locus_tag="OQN12_001763"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1654125.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27481..28500)
                     /gene="gpsA"
                     /locus_tag="OQN12_001764"
     CDS             complement(27481..28500)
                     /gene="gpsA"
                     /locus_tag="OQN12_001764"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1654126.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28500..28967)
                     /gene="secB"
                     /locus_tag="OQN12_001765"
     CDS             complement(28500..28967)
                     /gene="secB"
                     /locus_tag="OQN12_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1654127.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29030..29281)
                     /gene="grxC"
                     /locus_tag="OQN12_001766"
     CDS             complement(29030..29281)
                     /gene="grxC"
                     /locus_tag="OQN12_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1654128.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29423..29854)
                     /locus_tag="OQN12_001767"
     CDS             complement(29423..29854)
                     /locus_tag="OQN12_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1654129.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30099..31643
                     /gene="gpmM"
                     /locus_tag="OQN12_001768"
                     /gene_synonym="pgmI"
     CDS             30099..31643
                     /gene="gpmM"
                     /locus_tag="OQN12_001768"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1654130.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31653..32936
                     /gene="envC"
                     /locus_tag="OQN12_001769"
     CDS             31653..32936
                     /gene="envC"
                     /locus_tag="OQN12_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1654131.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32940..33899
                     /locus_tag="OQN12_001770"
     CDS             32940..33899
                     /locus_tag="OQN12_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1654132.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33886..34088)
                     /locus_tag="OQN12_001771"
                     /pseudo
     CDS             complement(33886..34088)
                     /locus_tag="OQN12_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8470 a         8931 c         8689 g         7998 t
ORIGIN      
        1 cttgagcgta atgcctttta tgaaggcgtc gaatttcctg atgattgtga tgttttaatc
       61 agctacgctc caccaaaaga tgaatcgctg gttgccagtt ttattacaca atatgccgta
      121 accgcttatg ccagccagcg ctatcttgag aaacatccta ttagccgtcc tgacgaactc
      181 gaacatcact cctgtatttt gatcgactcg atgatgatcg acgacgcgaa tatctggcgc
      241 ttcaacgtcg ccgggagtaa agaggtgcgg gattatcgcg tgaagggaaa ttacgtttgc
      301 gataacacgc aatcggcgct ggagctggca cgaaatcacc tggggattgt gtttgcgcca
      361 gacaaaagtg tgcagagcga tctgcaagac ggcacgctgg tgccctgctt tcagcacccg
      421 tatgaatggt ggctggatct ggtggctatc ttccgtaaac gggaatacct gccctggcgg
      481 gtccagtatg tgctggatga aatgctgcgt gaaattcgcc accagcttgc tcagtcgcag
      541 caactgcggc ctgaacaagc ggcggaaagc gaagattaat gatcgagata aaggtaatgc
      601 acccagctgg tcatgcgtaa tagcactttg cgcatcaccg agacatggct gaaatgatca
      661 gagtagaccg ccacctgggc gtaaataccg tctggtaaag cgctcaaatc agtgtgctca
      721 tccagttcaa tggtcgcgat aacgccatct gaacccggcg ctgtgtttaa cgtctgtaag
      781 gtgccggtcg actgataagc tccgccggga acagcaggac taatggctgc cagcttgccg
      841 ctgaatacct tacctggcag agcattaaac accacttccg catcgtcgcc aggggccagt
      901 cgcagcaagg agttctgacg gaactgcgcc acaatttgtc gtttctgatc gggtataaac
      961 accatcaccg gacgtagcgg cagcgacgcg gcataggtac ccggacgaat cagcacctgg
     1021 gtcacataac catcgctcgg cgcacgcact atcgtctgct caaggttata ttttgcttcc
     1081 gcgagctgcg ctttcaggct ggcgatttga gaatgttcac ccaacaccag gctatccagc
     1141 tggctctgga tctgtttttg ttccgccgcc gatgacttta cggaggcttc ctgcgccaga
     1201 taattttgcc gcgccacatc aatatcgcgt tctgaaaacg ggtttacttt cgcctggctg
     1261 ccgcgtgcgt aacgctgata ctctttagcg aatttatccc gcgtggcctt tgcctgctga
     1321 gtattcgccg ccatctcatc taactctgcg cccaacgccc gctgtttatg ttctgcggta
     1381 acgatatccg ccatcagccg atccacccgc gcctgataac gcgtcgggtc cagtcgaaat
     1441 agcacctcac cttttttaat cagcgtattt ttcttatccg tcacttcgat caccacgcct
     1501 gtcacctgtg ggacaacagg aatagaaata accgcttttt gcgccttgaa ggtatacgga
     1561 tggttatagt tcattaacag aattaaacca ctgacaataa atatgccacc cagggccgct
     1621 gtggaaatgg tccatttatt tacaggaatt ttgaagatct taaacattgc ccatgcaaaa
     1681 gccacatagg tcaaaataat cagtaaatcc ataagtatta ctcggcagaa gaggatttga
     1741 tgtcggcgag ctgtttctcc agcccggcaa tacgctgttg tagttgcaca acggaggagt
     1801 catggatttg cattccccat ccccgctccg ggcggtaaag cgtggcccaa atccacaaaa
     1861 atggccagat aacatgcagc gtaaaaagac tcacccaacc agcaacatga atggcgtcgg
     1921 catgaggatg attacgtttt ttagcaatca ggtagggaat atcatgtaaa ataataatcc
     1981 catagaatat caccagaaat acaaaaataa gcactcccag tgcaaaatag tccaggaaca
     2041 tatttccctc aaagaatata aaaaagaaca attaacgcat attatgaaat gccatgatgc
     2101 agtgcaattt ccttatttat ataacgacat attgtttttt atatactttc tgaaatgtgc
     2161 tgtctggttt ttcaacagga cgaggtttat catattgaaa tagtggaact attggccaaa
     2221 ctaatgaata actccagtaa aacatccaca gtacagatta agcgtattaa accttcaatt
     2281 atctaccgtt tattgctgat tggcctcgga tcaccaatgg tgatttacgg cctggttcgc
     2341 ccgctcacca tcgaaacgcg agattaaact cctgacaaaa cacgccccag aaacgcactt
     2401 ctgttggcgt agtctgggtt attgcgcacg taggtttaaa acgtaaattg taacaacgtg
     2461 cgctttgttt atgccggatg cggcgtgtac gccttatccg gcctacggtc aggttcccgt
     2521 aggcatgata agacgcgtaa gcgtcgcatc aggcaatgaa tacccaatgc gaccagcttc
     2581 ttatatcaga acagccccaa cggtttatcc gagtagctca ccagcaggca cttggtttgc
     2641 tggtaatgct ccagcatcat cttgtgggtt tcgcgaccga tacctgattg tttgtagcca
     2701 ccaaacgcag catgtgccgg gtaagcgtga taacagttgg tccacacgcg cccagcctgt
     2761 atgccgcgcc ccatcttata ggccagatta ccgttgcggc tccagacgcc cgcgcccagg
     2821 ccatattgcg tatcgttcgc cagctccagc gcttcttcca tcgttttgaa ggtggtcacc
     2881 gccagcaccg ggccaaaaat ctcctcctgg aacacccgca tattgttctg accaaacaga
     2941 atcgtcggtt cgaggtagta gccgtctttc agttcacctt ccagcagctt gcgccgcccg
     3001 cctgtgagca cgtcagcgcc ctctttttta ccgatatcaa tgtagttgag gatggtttcc
     3061 agttgcccgt gagaaacctg cgcgcccatt tgcgtcacgc tgtcgagcgg gttaccgctg
     3121 cgaatgcttt cgacacggcg gatggcgcgt tccataaagc gttcgtagat tgactcctgc
     3181 actaaggcac gactcggaca ggtgcaaact tcgccctggt taaaggcaaa cagtgcaaag
     3241 ccttccagcg ctttatcgaa aaaggcatct tcttcatcca tcacatcagc aaagaagata
     3301 tttggcgact taccgcccaa ctccagcgtc accggaataa tgttttgcgt tgcgtattgc
     3361 ataatttgtt ggcccacttc cgttgagccg gtaaacgcca ctttggcgat gcgtttcgag
     3421 gtcgccagat attcgccaat ttccccacct gcgccattga ccacgttcac cacgcccggc
     3481 ggcagtaaat caccgacaat ttccattagc agcagtacag aaagcggggt aagacgtgcg
     3541 ggtttcagca ccacacagtt gcccgccgcc agcgcgggag ccattttcca gctcgccatc
     3601 agcagcggga agttccacgg gataatctgc cccaccacgc ctaacggttc atggaaatga
     3661 taggccacgg tttcgctatc aacttcactg atcccacctt cctgcgcccg aatacacgag
     3721 gcgaaatagc ggaaatggtc aatcgccagc ggtacatccg cagcactggt ttcgcgaatg
     3781 ggtttgccgt tatcccaggt ttcagctgtc gctaacagct cgaggttttg ttccattcga
     3841 tcggcaatct taaacagaat cgccgcgcga tcctgcaccg aggtgtgcgc ccatttatct
     3901 ttcactttgt gcgcagcatc cagcgccaga tcgatgtctc gtttgcccga agacgccact
     3961 tcgcacagca gctgcccggt caccggcgtc agattctggt aatactcacc atcggcaggg
     4021 gctacccatt caccgccaat aaagttgtcg tagcgggctt ttaacttgat ggggaaacca
     4081 tactcgccgg gcttaatctg tgctgaaggg ggattattgg tcatgatcgt ctccttgcgg
     4141 tgtgaggtat aacaagggta gacgtgactg gcgaaatctt cgccagtcgg taacagcttt
     4201 acgacagcta tcacgaattt acgggcaagg attacagaga tagttgaaaa agcggctgac
     4261 aatttgccag ccgttgttgg aatgatgatt acattgcggc gcgataaatc accacaattt
     4321 cttcgtgagt tgcctggatc gggttagtaa agccacaggc atctttcagg gcattagtcg
     4381 ccagaaccgc gaaatcttct tctttcacgt tcaggtcgcg taggcctgcc gggatatcca
     4441 ctttcttcgc cagttcacgg atggcgttaa tgcaggcttc agcaccttcc gcgtcgtttt
     4501 tacctgtcac gttcacgccc attgcagcgg cacagtcacg cagacgtgca gcggcgactt
     4561 tgctgttgaa tacctgaaca tgcggcagca gaacggcgtt acatacaccg tgtggcaggt
     4621 tgtagaaacc gcccagctgg tgcgccatcg catgaacata acccagagaa gcattattga
     4681 acgccatacc ggcaaggaac tgggcataag ccattgcttc acgcgctttc gcattactgc
     4741 catcttcaac ggctaacggc aggttttcgg caatcatggt cacggctttc agtgcacaag
     4801 cgtcagtgat cggcgtggcg gcaatagaaa catacgcttc gatagcgtgc gttaaggcat
     4861 ccatacccgt tgcggcggtc agtgacttcg gcataccaat catcagagag gagtcattga
     4921 cagaaagcag cggagtcacg tgcttatcaa caatcgccat tttgttatga cgcgcttcgt
     4981 cagtgatgat gcagaaacgg gtcatttcag aggccgtacc cgccgtggta ttgatggcga
     5041 tcatcggcag ctgcggtttt gcagagcggt caacgccttc gtaatcgcga atatcgccgc
     5101 cattggctgc caccagcgca ataccttttg cgcagtcgtg tggagaacca ccgcctaagg
     5161 agatcacgct atcgcaatta ttttctttaa gtaatttcaa acctgcggcg acgttttccg
     5221 tggtggggtt aggttgggtg ccatcataaa taacgctaaa aatattgcgt tcttccagtg
     5281 ctttttgcac atcacccgcc atacctaatt tcgttaacat attgtcagtg acaattaagg
     5341 tacgggtaaa tccataatct gccatcatat tcattgcatc agtcaatgaa tcagcgccga
     5401 tgacattcac agaaggaata aagaaagttg aagatgccat aacacactcc tgaaagtgta
     5461 aaaaagggtg ggcagcttac tgaggatttt cattcgaaaa catgatcatt attattaatt
     5521 atcaaaatta atctaataaa aagcacattg tattcattaa acaatgtgct ttatttaaaa
     5581 tcataagaaa tttgtattaa tttattttaa aaattaatta atcatttcct tatttttccg
     5641 gaaataataa tgcgtcgcgc aataaatgat catttccacg acgacgcgta aagccaatgc
     5701 ggtcaaaata ttccagaatc tgaattgcca gctttcggcc tacgcctaag cgatcgcgga
     5761 aatccgccgc gcaggttgaa ccacactcct gatcgagatc gcggatcata ttggcaaatt
     5821 cgacaatccg atcgttacgg taataacgat ctttgacgat cgcggtaatt attccttgct
     5881 gcgccgcctg gcgtagcgtc aggcgcattg cctgctcgtc ggtacccgtc tcttttgcca
     5941 gatcacgcac ccaccacggc tcgtcaccaa acagcggctc tgctttttgc cagatggcct
     6001 gctgctcttc gctgaagccc gctttgtgat ccggcagatg cagccaaccg tgatgactga
     6061 ggatgtcgcc gctttcgcgc atcttttcaa tcagcaacag tactagcgct tcatcttcca
     6121 ttggcaacgc catacgtcgc agacgttcgc gcccaggccc aggttcatcg cgatgttgct
     6181 catgataagt cgctaatgtg tcgagaattt tccgttgcca gcgagcggca accggcgcat
     6241 tcaacaagct ataaccagcc tgaatatagc caggctgttg cagcagttcg cgcatccctt
     6301 cgccgttgag ctggcgcgcc caggcgaaat caggaagatt aaccgcgccg cgttgcaggt
     6361 gaacggctaa cgcttcgcta tcattctgtg ccctggcgag ggaagccagc cattgcagat
     6421 actctggttt acgtttaccg cgacgcggcg ggttaagcat cacgacgcgc gctccggcca
     6481 gcgtgttgcg ggcagagata tcgcgcaata ccaggcggtc gttatctgcc agccataacg
     6541 gggtgtcgaa gaccagttca gcaaggttat cttccagcag tgaaacgcgt cccgtgacgt
     6601 ggctggcggc gtggtgaata tgcagcggct gccactgggt cagcggtgca tgggtttgaa
     6661 gctcgacaat cacccgtgtg aacggctctg gcggcacatc ggcaagcagc cagtcgccac
     6721 ggttaatctg ctctttttcc gcatcacccg cgatgtcaag cgcgatacgc tgcccggcat
     6781 tggcggtttc tgttggctgg ttttgcgcat gcagcgcacg tacacgcatc ggtttattta
     6841 caccagtcag ccagagtgaa tcgcctacct tcacttcccc gcttaacgcc gtaccggtga
     6901 cgaccagccc ggcacctttt acggtaaatg cgcggtcaat cgcgaggcgg aaactatgtt
     6961 ggctggcgtg ctcgcgttcc ggcaactgaa gcagatgctc gcgcagggca tcaattcccc
     7021 gaccttcggt tgctgcggtg ataaacagtt ttgcctcagc aaaaccgtat tcccgcagaa
     7081 cctcctttac ctggcgttca acctcatcaa cacgcgcttc gtccacgcga tcggctttgg
     7141 tcagcgccac tgtcagcatc gggttaccgg tcagctgcaa aatcgccaga tgctcacggg
     7201 tctgtgccat cacgccgtca tcgcacgcca ccaccaacag cgcgtgatcg ataccaccaa
     7261 cgcccgccag catgttggaa agaaactttt catgaccggg aacgtcgata aaaccaggca
     7321 cgcgaccatc cggctgcggc cagtaggcat agccgagatc gatggtcatg ccgcgctttt
     7381 tttcttccgg cagacggtca gcatttacgc cagtaatcgc ctgcaataag gttgttttgc
     7441 cgtggtcaac gtgtccggca gtcgcaataa tcatttcaac aacatctcca aaaaccgttg
     7501 ctcatcttca aggcagcgta aatccagcca caatcgtccg tcataaatac gaccaatcac
     7561 cggcactggc aattcacgcc agcgggcggc taatgactca aggtggctac cgcgtccatc
     7621 atggggtgta aacgttaatg ccgcgctcgg caggcgatca accggcagcg aaccactgcc
     7681 aatctgcgaa agacatggca taacctgtac cgcaaactcc gcgccgtaat gtgcgacaag
     7741 gggagcctgt aaacgttgtg cctggatttg aatgacctct gcgctgcggg taagcaggcg
     7801 cagggtcggt aatttttcac tcagagcttc agggtgtaaa taaagacgca acgtggcttc
     7861 cagcgccgcg agggtcattt tatccgcgcg taatgcacgc ttcagcgggt ggctttgcag
     7921 gcgggcgatc atctcttttt taccaacgat aatcccggcc tgcggcccgc ctaacaactt
     7981 gtcgccggag aagctcacca gactgacgcc cgccgcaatc aactcctgcg gcattggctc
     8041 tttcggcaaa ccgtactggc taagatcgac cagcgagcca ctgcctaaat cagtcaccac
     8101 tggaacatcc agctctttgc cgagcgccac cagttcagct tcgtctatcg ctttggtgaa
     8161 cccctgaatg ctgtagttac tggtatgtac tttcatcaac agtgcggtat tttcattcac
     8221 cgcctgacga taatcattcg cgtgcgtgcg gttggtggtc cctacttcgt gtagggtgca
     8281 gcctgcctga cgcataacat cgggaatacg aaacgcgccg ccaatctcca ccagttcgcc
     8341 gcgagatacc accacctctt tcccgctggc agtggccgcc aacatcaata acaccgccgc
     8401 cgcattgtta ttgacgatac aggcatcttc cgcccccgta atacggcaca gcagctgcgc
     8461 cagcgcccga tcgcgatgtc cgcgtccggc gtcgtccaga tcgtactcga gggtcactgg
     8521 cgaacgcata gcctgcgcaa cggcttccac cgcggcttcc gcctgtaaag ctcgcccaag
     8581 gttggtatgc agcacggttc ccgtcaggtt gatcaccgga cgcagcgcgc tctgcgcctc
     8641 tttcgtcaac cgggcatcga cttcttgcgc ccagttttca caccacgcag gcagcgtctg
     8701 gctgccacga atcacttctc gcgcttcgtc gagcatctga cgcaacaatt ccaccacgcg
     8761 ggtgtgacca taagtatcac gcaaagaaag gaaggagcta tcgcgcaata agcgatcaat
     8821 agccggaagt tgactataga gggaacgcgt ttcggttgtc ataggaaacc tggctgatca
     8881 aggccctctc acacggagaa gggcgtttaa cataaccacg gattgtaacg tgagatgggt
     8941 caggaggaca tatcgcgcat caagcctttg gcggttcggt gcgagcaaaa ctttcgcggc
     9001 taaacaggtt ttcgaccagt ttgactaagt gtgggcggtc gacacaccaa cctggcgcaa
     9061 cgcggcggaa attgagatat ccgacagcac aggcaatggc gatagtcgcc agattgaccg
     9121 tatcggtttt gagtgtgcca tcgaccagat atccttccag cacatccaga ctgcggttga
     9181 ttttctcccg ctggcgtaac aattcatctt cagactgctg cgccgccgga cgcgcctgtt
     9241 cacgcaccga taccagcccg gcatccataa tgccatccgc cagcgcctca attttgcgca
     9301 cccgcaacga ctccagcgga tcgcgcggca acatcgccgg agcgacattc attaactcaa
     9361 tatattcagc gatgatcggc gaatcaaacc agcattcgcc ctcttcggtc accagcaccg
     9421 gcactttccc taacgggtta aattgcgcca cgccgttatc cgcgttatag ggcagttcat
     9481 taataaattc gaaagttatg cccttttcta acaacagaat agaaagtttg cgtacaaacg
     9541 ggctggtgta gctaccgacg agtttcatgc cgagtccttt gtgcgaggaa aaatatcagt
     9601 atggctcagg ctcacgcaaa acacaggtga tataagtctc aatgatccaa ataaagataa
     9661 tgcatccaac tggtcattct tagcagcact ttccgcatca ccgaaacatg gctgaaatgg
     9721 tcggagtaaa ccgccacctg ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta
     9781 gggtccagtt caatggttcc cagcacaccg tccgtgccgg gaacgaccgt taatgattgc
     9841 aataccccct gcgcctgata agaaccgcct ggcacgacag gtaaaatact agtcaatttg
     9901 ccgtggaaca cctgcccagg tagcgcgtta aacaccactt ccgcatcatc gccaggtttc
     9961 agacgtaaca gcgagttttg ccgaaattgg gcgacaattt gccgtttttg ctcggggatg
    10021 aagaccatca ccggacgcag cggcaaggca gctgcgtatg tacctgggcg gatcagtacc
    10081 tgagtgacgt agccattgct tggtgcgcga atgacagtct gctcaagatt atattttgct
    10141 tcagtaagtt gcgctcttaa gctcacaatc tgcgattgct cgccgttgac catactgtcg
    10201 agctgactct ggatctgcgc ctgctccgcc accgagcctt tcactaacgc atcctgcgcg
    10261 aggaaatttt gccgcgcatc gtcgatgtca cgttccgaga acgaattcac cgccgcctgg
    10321 ctgcctttca ggtaacgttg ataattttta aacagacggt cgcgctccgc tgaaacctgg
    10381 gtggtgttgg cctgcgcttc agtgagctgc gcacgcagcg tctttatatt atgcgtcgcc
    10441 gtcatcaggt cagcctgaag tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta
    10501 aaaagcacct cgcccttttg aataagctga ttattcttgt cagtgacttc agtaacaatt
    10561 cccgtcacct gtggcgtgat agggatcgct atcactgcct tttgcgcggt aaaagtgtaa
    10621 gggtggttgt agttcatcaa caaaatcaaa ccactcacca gaaacacgcc tcccagcgcc
    10681 gccgtcgcca gcgccacgta agttaaaaca atcaatagat ccataattag atctccggga
    10741 acgtggaata gtcagtcttt ttctcggcgg cgagttgatg ctccagccgg gaaattcggt
    10801 cagaaagtgc ggcgatttcc ggttcagtcg ctttctcctg cgacgcaaca tgtgactgca
    10861 taccccagcc acgctccggt tgatagagcg tcgcccagat ccacagaaac ggccagataa
    10921 catgcagagt aaacaggctc acccagcccg ccgtatgaat agcgtcggca tgggggtgat
    10981 tgcgcttttt ggcaatcaga taagggatgt catgtatcgc gatgattcca taaaaaatca
    11041 ccagaaagac gaagatcagc actcccaacg cgaaatagtt tagaaacata tctgcctcgg
    11101 attcacgttt atcagtgttg ttttttggct ggcagccaga agggagtcag gctgatattt
    11161 tgacaataat ccgggttcgc gattctcgcc ataacaccaa agaataattt ttagaggtga
    11221 tgagttgctt agttacataa cgattgtatg acgaaggcat aacatgctgt agatcacatc
    11281 aggtgaacgc cgtaagaaaa tatcttgtga ttcagatcac aaagattcaa caaaccatca
    11341 aaacaaaaat gtgacattac tcacatttaa atgccatttt tagcgaaaat caccgccttg
    11401 tttctttttt atacaggcgt tttgtgatga gcgtcacgtc aattacctct ctacccccta
    11461 tatttatgtg actgatatca cacaaaagcc cgtcgactgg acagttaacc gattcagtgc
    11521 cagatttcgc agtatctaca aggtccggct acctctgccg ccacattaac aaaaaacctc
    11581 gggcttccag cctgcgcgac agcaaacata agaaggggtg tttttatgtc atccgatatt
    11641 aagatcaaag tgcaaagctt tggtcgtttc ctcagcaaca tggtgatgcc aaatatcggc
    11701 gcgtttatcg cgtggggtat catcaccgcg ttatttattc caacagggtg gttaccgaac
    11761 gagacgctgg cgaagctggt cgggccgatg atcacttatc tcctaccgct gctgatcggt
    11821 tataccggtg gtaagctggt aggcggcaaa cgtggtggcg tagtcggtgc cattaccacc
    11881 atgggcgtta tcgtcggcgc agacatgccg atgttcctcg gttctatgat tgcaggtccg
    11941 ctgggcggct ggtgcattaa gcacttcgac cgctgggtag acggtaagat caaatccggt
    12001 tttgagatgc tggtgaataa cttctccgca ggcatcatcg ggatgatcct cgctattctg
    12061 gcattcctcg gcattggccc gattgttgaa gccctgtcca aaatgctggc tgcgggcgtt
    12121 aacttcatgg ttgtccatga catgctgccg ctggcgtcta tctttgttga accggcgaaa
    12181 atcctgttcc tcaacaacgc cattaaccac ggtatcttct cgccgctggg tattcagcag
    12241 tcccatgaac tgggtaaatc aatcttcttc ctgattgaag ctaacccagg tccaggtatg
    12301 ggcgtgctgc tggcgtacat gttctttggt cgtggtagcg ctaaacagtc tgcgggcggt
    12361 gcggcaatca tccacttcct gggtggtatc cacgaaatct acttcccgta tgtgctgatg
    12421 aatccgcgtc tgatcctcgc agtcatcctc ggcggtatga ctggcgtgtt cacgctgact
    12481 atcctgggcg gtggtctggt ttctccggca tctccgggtt ctatccttgc tgtactggcg
    12541 atgacaccaa aaggtgctta tttcgctaac atcgcgggtg tgtgtgcggc gatggctgtc
    12601 tccttcgttg tctctgctat tttgctgaaa accagcaaag tgaaagaaga agatgatatt
    12661 gaagcagcaa ctcgtcgtat gcaggacatg aaagctgagt ctaaaggcac atctccgcta
    12721 tctgcgggcg atgtgactaa cgacctgagc cacgtacgta aaatcatcgt tgcctgtgac
    12781 gccggtatgg gttcaagtgc gatgggcgca ggcgtgctgc gtaagaaaat tcaggatgca
    12841 ggtctgtcgc agatttcggt cactaacagc gcgatcaaca acctgccgcc agatgtggac
    12901 ctcgtcatca ctcaccgtga cctgaccgaa cgcgctatgc gccaggttcc gcaggcacag
    12961 catatttcgc tgaccaactt cctcgacagc ggcctgtaca ccagcctaac cgaacgtctc
    13021 gttgctgccc agcgccatac tgaaaacgaa gtgaaagtga aagacagtct gaaagacagc
    13081 tttgacgatt ccagtgctaa cctgttcaaa ctgggcgcgg agaacatctt cctcggtcgc
    13141 aaagcagcaa ccaaagaaga agcgattcgt tttgctggcg agcagctggt gaaaggcggt
    13201 tacgttgagc cggaatacgt tcaggcgatg ctggatcgtg aaaaactgac cccgacttat
    13261 ctgggtgagt ctatcgcggt gccacacggt acggttgaag cgaaagatcg cgtactgaaa
    13321 acgggcgtgg tgttctgcca gtacccggaa ggcgtgcgct tcggtgaaga agaagatgac
    13381 attgcccgtc tggtgattgg tattgcagcc cgtaacaacg agcacattca ggttatcacc
    13441 agcctgacca atgcactgga tgatgagtcc gtcatcgagc gtctggcaca caccaccagc
    13501 gtggatgaag tgctggaact gctggcaggt cgtaagtaat ccaatcctac cctctcctca
    13561 cggagagggt ttgggtgagg gaaaagcctc accccagccc tctcgggtaa aaacattgat
    13621 gaaggttaat actatgaaag cattacattt tggcgcaggt aatatcggtc gtggctttat
    13681 cggtaaactg ctggcagacg cgggtatcca actgacgttt gccgatgtca atcaggtggt
    13741 acttgatgcc ctgaatgccc gtcatagcta tcaggttcat gtggtcggtg aaaccgagca
    13801 ggtagatacc gtttccggcg tcaatgctgt cagcagcatt ggtgatgatg tcgttgatct
    13861 gattgctcag gtcgatttag tcactaccgc cgttggcccg gttgtgctgg aacgtattgc
    13921 tccggctatc gccaaagggc tggtgaaacg caaagaacaa ggtaatgaat ccccgctgaa
    13981 catcatcgcc tgtgaaaaca tggtacgcgg caccacgcag ctgaaaggtc atgtgatgaa
    14041 cgccctgccg gaagacgcca aagcgtgggt agaagagcac gttggctttg tcgattccgc
    14101 cgttgaccgc atcgtaccgc cttcggcttc ggcaactaac gatccgctgg aagtgacggt
    14161 agaaactttc agcgaatgga ttgtcgataa aacgcagttc aaaggcgcac tgccgaacat
    14221 cccaggcatg gagttaaccg acaacctgat ggcatttgtc gaacgtaaac tcttcaccct
    14281 gaacacgggt catgctataa ccgcgtacct cggaaaactg gccggtcatc agaccattcg
    14341 tgacgctatt ctcgacgaga aaatccgcgc ggtggtaaaa ggtgcgatgg aagaaagtgg
    14401 tgcggtactg atcaagcgct acggctttga cgcagacaaa catgcggcgt acatccagaa
    14461 aatcctcggt cgttttgaga acccgtatct gaaagatgat gtagagcgcg taggccgtca
    14521 gccgctgcgt aaactgagtg ctggcgaccg tctgatcaag ccactgctcg gtacgctgga
    14581 atacagcctt ccgcacaaga atctgattca ggggattgct ggtgcaatgc acttccgcag
    14641 tgaagatgat ccgcaggctc aggaactggc agcactgatc gctgacaaag gtccgcaggc
    14701 ggcgctggca cagatttccg gtcttgatgc caacagcgag gttgtatccg aggcggtaac
    14761 cgcttataaa gcaatgcaat aatggtggac caggcgcagg acaccctgcg cccgaataac
    14821 agattgtcag atatgcaggc aacaatggaa caaacccagg ccgttgaaaa ccgtgtgctt
    14881 gagcgtctga atgctggcaa aaccgtgcga agctttctga tcaccgccgt cgagcttttg
    14941 accgaggcgg taaatcttct ggtgcttcag gtattccgca aagacgatta cgcggtgaag
    15001 tatgctgtag aaccgttact cgacggcgac ggtccgctgg gcgatctttc tgtgcgttta
    15061 aaactcattt acgggttggg cgtcattaac cgccaggaat acgaagatgc ggaactgctg
    15121 atggcattgc gtgaagagct aaatcacgac ggcaacgagt acgcctttac cgacgacgaa
    15181 atccttggac cctttggtga actgcactgc gtggcggcgt taccaccgcc gccacagttt
    15241 gaaccagcag actccagttt gtatgcaatg caaattcagc gctatcaaca ggctgtgcga
    15301 tcaacaatgg tcctttcact gactgagctg atttccaaaa tcagcttaaa aaaagccttt
    15361 caaaagtaag caacgtctgc ttactgcccc tctacctgct tcggccgata aagccgacga
    15421 taatactcca gacgttgtag atataacggc acgctttcat cgggtattcc ggacggaatc
    15481 gcgttacggg gaggaagttt tttcagatac tcccggaacg cctggcttga tgccatgaaa
    15541 tctacggctt tatcgataag aagcggaacg ttttcaccta atttgcccat gatgatatct
    15601 ccgtattacc ccgcgccggg aatgcgcggc ctccaatttt agtttaggtt cccggtaaag
    15661 atgacattaa ggaaaacgtg ctgaatcctc aataatcatc cgctgctgtt tattatcttt
    15721 ttctatagat ttcttaatta atcaacgaat tgcgtagttt ttgagcatgt taatgattac
    15781 gttgcagaac tattacaatg tcatcagttg tgaacaaagc acctggtcgc gcatactagg
    15841 ggctataaat ttatctttat cagaagccat cacatgaaag aagtcgaaaa aaacgaaatc
    15901 aagcgtctca gcgatcgcct ggacgccatc cgccaccagc aggccgatct gtcgctggtt
    15961 gaagccgcag acaaatatgc cgagctggaa aaagagaaag ccacgctgga agcagaaatt
    16021 gctcgcctgc gtgaggttca tagccaaaaa ctgagtaaag aagcacagaa gctgatgaag
    16081 atgccgttcc agcgcgcaat taccaaaaaa gagcaggctg atatgggcaa gctgaagaaa
    16141 agtgttcgcg gactggtggt tgtgcaccca atgaccgcgc tggggcgcga aatgggcctg
    16201 gaagagatga ctggattttc taagactacg ttttaagccc ctcagacgac tggttcgatc
    16261 gtatcaaccc gcatctggct tgccttagct ggtcaatgcg gggactctcc tgtctactcg
    16321 cctaattacc aaacaattca ctccctttac ttcgatttac cgctggttga tattctgaaa
    16381 atgcattttt accgaaatag ccatctataa acaataaaaa tatatagaag atatcatttg
    16441 ttaattaaca agcatttacg tctctttttt tcggatttaa ttgtgaaatt ttaatcatca
    16501 tattcacaaa atcaattatc tgtgaaattt ttcccagaca tggtttcttt catatttgac
    16561 atttattaat aatcatcatt agaatccacg aaaaataata tattcataca aacacagaca
    16621 tcttttgaaa cgaatgatac atttttatga tgaatgctta aaacaatatt taatcgacac
    16681 acatcactgt cattaatgta aagcaacata gctcaccaat aatacaatgg acaactctac
    16741 cggcattgat cgctggtcgg aggaaataag gatttttaca tgaacttgaa gaagattttt
    16801 ttcagtgctg tcactgtttc agttctctgc gcattaacgg gatgtgacta cattgaagaa
    16861 ggcaagccag aaagtagttt actgaaacaa caagaagaac ataataataa aattgtgcta
    16921 cttgaaaaac aacaagcaca gctaaagagt caactcgaaa ctattcaaaa acaacaaact
    16981 ggcataataa acagtacaaa gactttaact cacgtaatta aatccgttaa agaccaacaa
    17041 aatactttta tttttaccga gtttaaccca gcaaaaacga agtatttcat cctgaacaac
    17101 ggttcggtgg ctttagcggg tcgagtgtta tccattgacg ccacagagaa tggtagtgtt
    17161 attcatatct cactggtcaa cttattaagt acacctatct caaacattgg ttttaatgcg
    17221 acatggggtg gtgaaaaacc tgtcgatgcc aaagagtttg cccggtggca acaattactt
    17281 ttcaacacat caatgaaatc cacattgaaa ttattaccag gtcaatggca agacattaat
    17341 ttgaccctga agggtgtatc gcccaataac ctgggatatc tgaagttagc catcaacatg
    17401 gaaaatattc agttcgacaa tcttccctct gctgaaaatc ggcagaaaag aagcaaaaaa
    17461 taaatgcagt tagtacgctt ataacaagga atagcgagca tcattaatga acaaaatatt
    17521 taaagttatc tggaaccctg cgacagggaa ttatactgtt accagcgaaa cggcaaaaag
    17581 ccgtggcaag aaatctgggc gcagtaagct gttaatttct gcgctggttg cgggtggaat
    17641 gttgtcgtcg tttggggcat tggcgaatgc cgggaatgac aacggtcagg gtgttgatta
    17701 cggtagtgga tcagctggcg acggctgggt tgctataggc aaaggggcga aagcaaatac
    17761 ttttatgaac accagtggtt ccagtactgc tgtgggttat gacgctatag ctgaaggcca
    17821 atatagctct gccatcgggt caaaaaccca tgcgattggt ggtgcatcaa tggcctttgg
    17881 ggttagtgca atatcagaag gcgatagaag tatagcactg ggtgcctctt cgtattcatt
    17941 gggccaatac tcaatggccc tcggccgtta ttcaaaagca ttgggtaaat tgtctattgc
    18001 tatgggggac tcttccaaag cggaaggagc aaacgccatt gccctggggc gtagcagtgt
    18061 agctagcggt actgacagcc tcgcatttgg cagacaatca cttgccagcg cagcgaacgc
    18121 tattgcgata ggtgctgaga ccgaagccgc tgaaaatgca actgctattg gcaataatgc
    18181 gaaggcaaaa gggactaata gcatggcaat ggggttcgga agccttgccg ataaagtcaa
    18241 tactatcgca ttaggaaata gcagccaggc tctggcagat aatgcaatcg ccataggcca
    18301 gggcaacaaa gctgatggcg tggatgccat cgctctgggt aatggtagcc agtcgagagg
    18361 cttaaacacc attgccttag gcacagccag taatgcaact ggtgataaga gtcttgcgct
    18421 tggtagtaat agcagtgcca acggtattaa ctctgtcgcg ctgggcgcag attccattgc
    18481 ggatttagac aataccgtct ctgtcggcaa tagttcatta aaacgcaaga tcgttaatgt
    18541 gaaaaatggc gcgatcaagt ctgacagtta cgatgccatt aatggttcac agctttatgc
    18601 cattagcgac tcggtagcaa aaaaggcttg gaggaggggc tgcagtagat gttgatgacg
    18661 gtactgttac agcaccaacc tacaatttaa aaaatggtag caaaaataac gtaggggctg
    18721 cgctcgctgt acttgatgaa aacaccctgc aatgggacca aaccaaaggc aaatacagcg
    18781 ctgctcatgg tactagtagc ccaactgcca gcgtaatcac cgatgttgcg gatggcacga
    18841 tttcagcctc cagtaaggat gcggttaacg gttcccaact gaaagctacc aatgacgatg
    18901 tcgaagccaa caccaccaat atcaccaacc tgacggattc cgttggtgac cttcaggctg
    18961 atgccctgct ctggaacgaa actaaaaagg cattcagtgc agctcacggc caggatacca
    19021 ccagcaaaat caccaacgtt aaagatgccg acctgacggc tgacagcact gatgctgtta
    19081 acggctctca gctgaaaacc accaacgatg ctgtggcgac gaataccacc aatatcgcca
    19141 ataacacttc caatattgcc actaacacca ccaacatctc taacctgact gagacggtga
    19201 ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtattc acggcagctc
    19261 atggcaccga gaccaccagc aaaataacca acgttaaaga tggcgacctg acgactggca
    19321 gcaccgatgc cgttaacggc tctcagctga aaaccaccaa cgatgccgtg gcgacgaata
    19381 ccaccaatat cgccactaac accaccaaca tctctaatct gactgagacg gtgactaatc
    19441 ttggtgagga tgcgctgaaa tgggataagg acaatggtgt cttcactgca gctcatggca
    19501 acaataccgc cagcaaaatc accaatatcc tggacggcac agtcactgca accagttccg
    19561 atgccattaa cggtagccag ctttatgact taagcagcaa tatcgccacc tacttcggcg
    19621 gcaatgcttc tgtgaatact gacggtgtgt ttaccggtcc aacctacaaa atcggtgaaa
    19681 caaattatta taacgtcggc gatgcactgg ctgcgattaa ctcctcattt agcacgtctc
    19741 tcggcgatgc tctgctttgg gatgccaccg caggtaaatt cagtgccaaa cacggtacta
    19801 atggtgacgc aagcgtgatc actgatgtcg cagatggtga aatttcagac tccagttctg
    19861 acgcagtaaa cggctcacaa ctccacggcg tgagcagtta tgttgttgat gcgctggggg
    19921 gtggtgccga agtcaatgca gacggcacca tcaccgcacc gacttacacc attgcgaacg
    19981 ccgagtacaa caacgtcggt gacgccctgg atgcgcttga tgataacgcc ctgctgtggg
    20041 ataagactgc caatggcggt gctggagcct acaatgccag ccatgacggt aaagccagca
    20101 tcatcactaa tgtcgctaat ggcagtatta gtgaggacag taccgatgca gtgaacggtt
    20161 ctcagttgaa tgcgacgaat atgatgattg agcagaacac ccaaattatc aatcagctcg
    20221 ctggtaacac cgacgcaacc tatatcgaag aaaatggcgc tggtatcaac tacgttcgta
    20281 ctaacgacaa cgatttagcc tttaacgatg caagcgcctc tggtgtcggc gctacagctg
    20341 taggttataa cgctgtcgcg tctggtgcca gcagcgtagc cattggtcag aacagcagca
    20401 gcaccgttga taccggtatt gcgctgggta gcagctccgt ttccagccgt gtgattgcca
    20461 aaggttctcg tgacactagc gtaacggaaa atggcgtggt gattggttat gacaccactg
    20521 acggcgaact gctaggtgca ttgtcaattg gtgatgacgg taaataccgc caaatcatca
    20581 acgtagctga tggttcagaa gcccatgacg ccgttacggt tcgccagttg cagaacgcta
    20641 ttggagcggt cgccactacg ccaaccaagt actttcacgc caactcaacg gcagaagact
    20701 cactggccgt tggtgaagac tcgctggcaa tgggtgcgaa aactgtcgtt aatggtaatg
    20761 caggtattgg tatcggtttg aacactctgg ttctggctga tgcgatcaac ggcattgcta
    20821 tcggcagtaa cgcacgcgca aaccatgcaa acagtatcgc aatgggtaac ggttctcaga
    20881 ccacccgtgg tgcacagact ggctacaccg cctacaacat ggacgcaccg cagaactctg
    20941 taggtgagtt ttctgtcggt agtgaagacg gtcaacgtca gatcacaaac gtcgcagctg
    21001 gttcagcgga taccgatgcg gttaacgtgg gtcagttgaa agtcactgat gagcgcgtag
    21061 cgcaaaatac ccagagcatt actaacctga acaatcaggt cactaatctg gatactcgcg
    21121 ttactaatat cgaaaacggt attggcgaca ttgtcaccac cggtagcacc aagtacttca
    21181 agaccaacac cgatggcgta gatgccaacg cccagggtaa agatagcgtt gctattggtt
    21241 ctggttccat tgctgccgct gacaacagcg tcgcactggg taccggttcc gttgcagagg
    21301 aagaaaatac aatctctgta ggttcttcta ctaaccaacg ccgtatcacc aacgtagctg
    21361 ccagtgttaa tgccaccgat gcggttaacg tgtcgcagct gaaatcttct gaagcaggcg
    21421 gagtgcgcta cgacaccaaa gctgatggtt ctatcgacta tagcaatatc accctcggtg
    21481 gtggcaacgg cagtacgact cgtatcagca acgtctccgc tggcgtcaac aacaacgacg
    21541 cggtgaacta cgcgcagttg aagcaaagcg cgcaggaaac gaagcaatac accgatcagc
    21601 ggatggttga gatggataac aaactgtcta aaactgaaag caagttgagc ggtggtatcg
    21661 cttctgcaat ggcaatgacc ggtctgccgc aggcttatac accgggtgcc agcatggctt
    21721 ctattggtgg cggtacttac aacggtgaat cggcagttgc tttaggtgta tcgatggtga
    21781 gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag ggtgaatact
    21841 ccgccgcact cggtgccggt attcagtggt aatgatccat taagttagtg tgactaaggg
    21901 caaacgccaa attgcctgat gcgttacgcg catcaggcaa gataaggcgc tccgggtcaa
    21961 caatctaagg gcaattctct gatgaggatt gcccttttct ttaccagaca tctcccccca
    22021 caagaattgg ccctaccaat tcttcgctta tctgacctct ggttcacaat tccccaatta
    22081 aaactcacat caatattgcc aatacataac atttagttaa ccattcattg tcattatccc
    22141 tacacaacac aattggcagt gccactttta cacaacgtgt gacaaggaga tgagcaacag
    22201 actcattaca cgatgtgcgt ggactctagg agacctgcaa tgaatctctg gcaacaaaac
    22261 tacgatcccg ccgggaatat ctggctttcc agtctgatag catcgcttcc catcctgttt
    22321 ttcttctttg cgctgattaa gctcaaactg aaaggatacg tcgccgcctc atggacggta
    22381 gcaatcgccc ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc
    22441 tcagtggttt atggcttctt ctatggtttg tggcccatcg cgtggatcat tattgcagca
    22501 gtgttcgtct ataagatctc ggtgaaaacc gggcagtttg acatcattcg ctcgtctatt
    22561 ctttcgataa cccctgacca gcgtctgcaa atgctgatcg tcggtttctg tttcggcgct
    22621 ttccttgaag gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc attgctggtc
    22681 ggcctgggtt ttaaaccgct gtacgccgcc ggactgtgcc tgattgttaa caccgcgcca
    22741 gtggcatttg gcgcgatggg cattccaatc ctggttgccg gacaagtaac aggcatcgac
    22801 agctttgaga ttggccagat ggtggggcgt cagctaccgt ttatgaccat tatcgtgctg
    22861 ttctggatca tggcgattat ggacggctgg cgcggtatca aagagacgtg gcctgcggtc
    22921 gtggttgcag gcggctcgtt tgccatcgct cagtacctta gctctaactt cattgggccg
    22981 gagctgccgg acattatctc ttcgctggta tcactgctct gcctgacgct gttcctcaaa
    23041 cgctggcaac cagttcgcgt attccgcttc ggtgacttgg gggcgtcaca ggttgatatg
    23101 acgctggccc acaccggtta caccgcgggt caggtgttac gtgcctggac accgttcctg
    23161 ttcctgaccg ctaccgtaac actgtggagc atcccgccgt ttaaagccct gttcgcatcg
    23221 ggtggtgcgc tgtatgagtg ggtgatcaac attccggtgc cgtacctcaa taaactggtt
    23281 gcccgtatgc caccagtggt cagcgaggct acagcctatg ccgccgtgtt taagtttgac
    23341 tggttctctg ccaccggcac cgccattctg tttgctgcac tgctctcgat tgtctggctg
    23401 aagatgaaac cgtctgacgc tatcagcacc ttcggcagca cgctgaaaga actggctcta
    23461 cccatctact ccatcggcat ggtgctggcg ttcgccttta tctcgaacta ttccggactg
    23521 tcatcaacac tggcactggc actggcgcac accggtcatg cattcacctt cttctcgccg
    23581 ttcctcggct ggctgggggt attcctgacc gggtcggata cctcatctaa cgccctgttc
    23641 gccgcgctgc aagccaccgc agcacaacaa attggcgtct ctgatctgtt gctggttgcc
    23701 gccaatacca ccggtggcgt caccggtaag atgatctccc cgcaatctat cgctatcgcc
    23761 tgtgcggcgg taggcctggt gggcaaagag tctgatttgt tccgctttac tgtcaaacac
    23821 agcctgatct tcacctgtat ggtgggcgtg atcaccacgc ttcaggctta tgtcttaacg
    23881 tggatgattc cttaatgatt gttttaccca gacgcctgtc agacgaggtt gccgatcgtg
    23941 tgcgggcgct gattgatgaa aaaaacctgg aagcgggcat gaagttgccc gctgagcgcc
    24001 aactggcgat gcaactcggc gtatcacgta attcactacg cgaggcgctg gcaaaactgg
    24061 tgagcgaagg cgtgctgctc agtcgacgcg gcggcgggac gtttattcgc tggcgtcatg
    24121 acacatggtc agagcaaaac atcgtccagc cgctaaaaac gctgatggcc gatgatccgg
    24181 attacagttt cgatattctg gaagcccgct acgccattga agccagcacc gcatggcatg
    24241 cggcaatgcg cgccacacct ggcgacaaag aaaagattca gctttgcttt gaagcaacgc
    24301 taagtgaaga cccggatatc gcctcacaag cggacgttcg ttttcatctg gcgattgccg
    24361 aagcctcaca taacatcgtg ctgctgcaaa ccatgcgcgg tttcttcgat gtcctgcaat
    24421 cctcagtgaa gcatagccgt cagcggatgt atctggtgcc accggttttt tcacaactga
    24481 ccgaacaaca tcaggctgtc attgacgcca tttttgctgg tgatgccgac ggggcgcgta
    24541 aagcaatgat ggcgcacctt agttttgttc acaccaccat gaaacgattc gatgaagatc
    24601 aggctcgcca cgcacggatt acccgcctgc ccggtgagca taatgagcat tcgagggaga
    24661 aaaacgcatg attatttccg cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc
    24721 gccgttcctg ttccactata tggatggtgg tgcatattct gaatacacgc tgcgccgcaa
    24781 cgtggaagat ttgtcagaag tggcactgcg ccagcgtatt ctgaaaaaca tgtccgactt
    24841 aagcctggaa acgacgctgt ttaatgagaa attgtcgatg ccggtagcac tggcaccggt
    24901 gggtttgtgt ggcatgtatg cgcgtcgtgg cgaagttcag gcagccaaag cggcggacgc
    24961 gcatggcatt ccgtttactc tctcgacggt ttccgtttgc ccgattgaag aagtcgcgcc
    25021 agccatcaag cgcccaatgt ggttccagct ttatgtactg cgcgatcgcg gctttatgcg
    25081 taacgcgctg gagcgagcaa aagcagcggg ttgttcgacg ctggttttca ccgtggatat
    25141 gccgacaccg ggcgcacgct accgtgatgc gcattcaggt atgagcggcc caaatgcggc
    25201 aatgcgccgc tacttgcagg cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa
    25261 cggtcgtcca catgatttag gtaatatctc agcttatctc ggcaaaccga ccggactgga
    25321 agattacatc ggctggctgg ggaataactt cgatccgtcc atctcatgga aagaccttga
    25381 gtggatccgc gatttctggg atggcccgat ggtgatcaaa gggatcctcg atccggaaga
    25441 tgcgcgcgat gcagtacgtt ttggtgctga tggaattgtg gtttctaacc acggtggccg
    25501 ccagctggac ggtgtactct cttccgctcg tgcgttgccc gctattgcag atgcggtgaa
    25561 aggtgatatc gccattctgg cggatagcgg aattcgtaac gggcttgatg tcgtgcgtat
    25621 gattgcgctc ggtgccgaca ccatactgct gggtcgtgct ttcctgtatg cgctggcaac
    25681 agcgggccag gcgggtgtag ctaacctgct aaatctgatc gaaaaagaga tgaaagtggc
    25741 gatgacgctg actggcgcga aatcgatcag tgaaattacg caagattcgc tggtgcaggg
    25801 gctgggtaaa gagttgccta cggcactggc tccgatggcg aaagggaatg cggcatagtc
    25861 ttttgcgccc cctcacccta accctctccc tcagggagag gggaccgttc ggcgctgtat
    25921 gtactccctc actctgaaac gacaccgcac tctttttttc tccctcgccc ctccggggag
    25981 ggccggggtg aggggaaaag gccgcacggt tcaccaattt tctgctatcc tgcccccgca
    26041 ctaagggggc agtatgctaa acatcgtact ttacgaacca gaaattccgc caaatactgg
    26101 caacatcatc cgtctttgcg ctaataccgg ctttcgtcta catatcatcg aaccgatggg
    26161 atttgcctgg gacgacaagc gcctgcgccg cgcggggctg gactatcacg agtttaccgc
    26221 cgttacgcgt catcatgact atcgcgcgtt cctcgaagca gaaaatcccc agcgcctgtt
    26281 tgccctcacc acgaaaggta cgcctgctca cagcgccgta agctatcagg atggcgatta
    26341 tctgatgttc ggcccggaaa cacgcggcct gccagcgagc attcttgatg ccctgcccgc
    26401 tgaacaaaaa attcgcattc cgatggtgcc ggacagccgc agcatgaatc tgtccaatgc
    26461 ggtgtcggta gtggtgtatg aagcctggcg gcagctggga tatccgggag cgttgttgag
    26521 agattagtta ctgtatgccg gatagatgat tacatcgcat ccggcacgat cacaggacat
    26581 tagatcccat ccccatactc aaatgtatgg ttaataccgt tgaaatgctg gtccatatcc
    26641 attgatggct tatcgctgtc tggtttaccg acaatacgaa ccggaacgcc agcggcggtt
    26701 gtatgcggcg gcaccggttg cagcaccacg gaacctgcgc caatcttcgc gccgcgacca
    26761 acttcaatat tgccgaggat tttcgcgccc gcgccaatca tcacaccttc acgaattttc
    26821 gggtgacggt caccaccaga tttacccgta ccgccaagcg tcacagattg cagaatcgat
    26881 acgtcgtttt caattaccgc cgtttcaccc acaacgatgc ccgtggcgtg gtcgagcata
    26941 atgccacggc caatttttgc tgccgggtga atatcgacct ggaacgtcac agaaacctgg
    27001 ttttgcagaa agattgccaa tgcgcgacgc ccctgattcc acaaccagtg accgatgcga
    27061 taggcctgca aggcatgaaa acccttcagg tataacaacg gggttgagta tttatcgact
    27121 gccgggtcgc gggtacgcac cgcctgaata tcacaggccg cagaggcgat catttccggg
    27181 tcagcggcgt aggcttcttc aaccacttca cggatagcaa tagcaggcat aattggcgat
    27241 gacagcttgt tcgccagcat gtagctcagt gcactgccaa ggttttcgtg cttgagtagc
    27301 gtcgcgtggt aaaaactggc cagcattggc tcacagtccg ccagcgttct ggcttcggct
    27361 ttaatattgt tccagacaat ttccagttct tcacacgaca ttgcttactc cacacgatga
    27421 gataatgacc ggcccgttct gcgcgggccg ggtcatagcg gtaacaaagg ttccctgggg
    27481 ttagtgactg ctgcgctcgt ccttgcgtgc acgacctaat aatgtcaatg ctgcctcgcg
    27541 cgcgtttttt ccgcaatata atacttgata aatttcctcg gttattggca tttcaacgcc
    27601 gaagcgatgc gccagttcgc ggacttcttt cgtattgcgg tagccttcca ccacctgacc
    27661 aatcttctcc tgcgcgcttt gtacatccat gccctgaccg agcatcatac caaaacggcg
    27721 gttacgcgac tggttgtcgg tacaggtaag caccagatcg cccagccccg ccatgcccat
    27781 aaaggtggca ggatcggcac ccagcgccgc accaagacgc gacatttcag ccagcccacg
    27841 ggtgatcagc gccgtacgcg cattcgcacc aaaaccgata ccgtcagaca tccccgcgcc
    27901 aatggcaatg acgtttttca ccgcgccgcc aagctgcacg ccaatgaaat ccgggttgct
    27961 gtaaacgcgg aaacttttgc cgcagtgcag cagctgctgg agatcatcgg caaaggtctg
    28021 atcggtcgag gccagcgaaa tagctgtcgg taaacctgcc gccagttctt tcgcaaacgt
    28081 tgggccagag ataaccgcca gcggaatttg atcgcctaag gcctcacgcg ccacgtcctg
    28141 taacagacgt ccggtttccg cttccagccc tttggtcgcc cacaccagac gcgcatcagg
    28201 acgcatcagc ggtttaatct ggcgcagcac ttcaccaaag acatggctgg gtacgacgac
    28261 gagaatatta cgactggctg ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc
    28321 gggaaaaggc acatcgggga gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat
    28381 atgttcaggg tcatggcccc agaggacaac ctcgtggcca tttcttgcca gggtaatggc
    28441 aagagcggtg ccgtacgagc cggcaccgat cacagtcatt gaagcattac gttggttcat
    28501 caggcatcct gatgttcttc agtaccttcg ccagcctgct gctgcaaata gttcatgaac
    28561 agcgcatcga agttaaccgg cgcaaggttc agttgcggga atgtaccgcg ggataccatg
    28621 ctggtgatgc actcacgagc atacgggaac agaatgttcg ggcagtatgc gcccaggcaa
    28681 tgtgccatct gggtgccttc gatacccgcg atggagaaaa taccgccctg ctgaacttca
    28741 cacaggaacg cggtttcttc gcccaaggag gccgttacgg taacacgcag taccacttcg
    28801 tatacgtcat ctgccagttg ggaagatgcc gtatccagat caagtttaac ttctggttgc
    28861 caatctttct ggaaaacgtg cggcgcgttc ggcgcttcga aagagatatc cttggtataa
    28921 atacgttgga tctggaaagt catttcagtg ttgttttgtt ctgacatgtg tagaaaaccc
    28981 ttaagtgttg tccttaaata ctgcgcaatg taataccaac gttcacacgt tatttcagca
    29041 ggggatccag tccaccacgt gcatccaatg catacaagtc atcacagccg ccaatgtgct
    29101 gtgcgtcaat aaaaatctgg ggaaccgtgg tgcaaccgct gcgtttgatc atctcttcac
    29161 gcttggcggc attgccatcg atcggcaact cctggaaact cacgcccttg ctgctcagca
    29221 gtgcttttgc acgatggcaa tacgggcagg tttctttggt atagatttca acattggcca
    29281 taatttagct cctgtttttt taccctgaaa tttcatgttg cagggcggct gcaaaatgga
    29341 gtgtccccag aagcttataa caataagtcg ctgggggcct aattgcagct aacggcctgc
    29401 atcatgaaag acgacaggta aattatttgc cgcgcaccag aggcaagttt tcgccagccc
    29461 agccagcaac gccctctttc agcacgaata cttgcgcaaa accagctttc gtcagcgcgt
    29521 ttgcaggctc ctggcactgc atgccagaac cgtctaccac gataaccgat ttgtctttgt
    29581 gcttctcaag ctcaccaaca ttgttggctt tgatttcgct cggcaacaga ttgatagaac
    29641 ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa atccacaacc acagcgtctt
    29701 ctttgttgat cagacgcgta gcttcaccac gagtaatcac cttcactttc gaggtcaggc
    29761 tcttaaacgt agtcacaaga accgccacca gtaacgcgat ccaggcgata ctcagtatgg
    29821 gatgacggcc aacaaattgc ataatttctt gcatgggggg taacaactcc cgacgtagtg
    29881 attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg caaatacagc cagcgcgtta
    29941 actggaatgc aattttgcgg ggcacgacga aaaaaaagcg caaatctggc atcggcttgc
    30001 cgcgagcggt cgtatatttt gatcttcaga ggctatttta tcgattcagc tgtagtaaaa
    30061 ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta
    30121 tggtactggt gattctggat ggctatggct atcgtgaaga acagcaggat aacgccattt
    30181 ttagtgctaa aaccccggta atggatgcac tgtgggccaa tcgtccgcat accctgatcg
    30241 acgcttccgg tctggaagtc ggtctgcctg accgtcagat gggtaactcc gaagtaggcc
    30301 acgttaacct gggtgccggc cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa
    30361 tcaaagatcg cgctttcttt gctaatccag tgctggctgg cgcggtacat aaagcgaaaa
    30421 acgcaggtaa agcagtacac attatgggtc tgctctccgc aggcggcgta cacagccacg
    30481 aagatcacat catggcgatg gtagaactgg cagctgaacg cggcgcagaa aaaatctacc
    30541 tgcacgcatt ccttgacggt cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa
    30601 aatttgaaga gaaatttgct gcgctgggta aaggccgcgt agcgtccatc attggtcgtt
    30661 actacgcgat ggaccgcgat aaccgttggg atcgcgttga aaaagcctat gacctgctga
    30721 ctctggcgca gggtgagttc caggccgata ctgccgttgc tggtttgcag gcagcttatg
    30781 ctcgcgacga aaacgacgag ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag
    30841 atgcggcaat ggaagacggc gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc
    30901 gtgaaatcac tcgtgcattc gtgaacgctg atttcgatgg cttcgcgcgt aagaaagtgg
    30961 ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga catcaaaact gcggttgctt
    31021 acccacccgc ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc
    31081 agttgcgtat ttccgaaacc gaaaaatatg cccacgttac tttcttcttc aacggtggcg
    31141 tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct
    31201 acgatctgca accggaaatg agctccgcag agctgaccga aaaactggtt gcggccatca
    31261 agagcggcaa atacgacacc attatctgta actatccgaa cggcgacatg gtaggtcaca
    31321 ccggggtgat ggaagcggcg gttaaagcgg ttgaagcact ggatcactgc gtggaagaag
    31381 tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat caccgctgac cacggtaacg
    31441 ctgaacagat gcgcgatccg gcaacgggtc aggcacacac ggcacacacc aacctgccag
    31501 ttccgctgat ttacgttggt gataagaacg tgaaagcggt tgcaggcggc aaactttctg
    31561 acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat cccgcaagag atgactggta
    31621 agccgctgtt catcgtggaa taatccctcc ccatgagggg aaaggcgatt aataccatga
    31681 cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc agcgttctta
    31741 gcgctggcgt attgttgtgc gccttttccg cccacgcgga tgagcgtgac caactcaaat
    31801 ctattcaggc cgatatcgcc gcaaaagagc gcgcggtacg ccaaaagcaa caacaacgcg
    31861 caagcctgct cgcacaattg aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc
    31921 tgcgcgaaac gcaaaacacg ctcaatcaac tcaataaaca gattgatgag atgaacgcgt
    31981 cgattgccaa actggagcag caaaaagccg cccaggagcg cagcctcgcc gcacaactgg
    32041 atgccgcatt ccgtcagggc gagcataccg gtattcagct aattctcagc ggtgaagaaa
    32101 gccagcgtgg acagcgttta caggcttatt tcggctatct caaccaggcg cgacaagaaa
    32161 ccattgccca gttgaagcaa acgcgtgaag aagtcaccat gcagcgtgct gaactggaag
    32221 agaaacaaag cgagcaacaa acgctgttat atgaacagcg cgcccaacag gcgaaactga
    32281 ctcaggctct gaacgagcgt aaaaagacgc tggcggggct ggaatcttcc atccagcaag
    32341 gtcagcagca gctaagtgaa ctgcgtgcca acgaatcccg tctgcgtaac agcattgccc
    32401 gtgcggaagc cgcggcgaaa gcgcgtgctg aacgagaagc gcgtgaggcc caggcggttc
    32461 gcgagcgcca gaaagaagcg acgcgcaaag gcaccaccta caaaccgacc gaaagcgaaa
    32521 aatcgctgat gtcccgaact ggtggcctgg gtgcgccgcg tggtcaggca ttctggccgg
    32581 ttcgcgggcc gacgctgcat cgctatggcg agcagctaca gggtgaatta cgctggaaag
    32641 ggatggttat cggtgcctct gaaggtactg aagttaaagc aattgccgac ggtcgagtga
    32701 ttctggctga ctggctgcaa ggttacggtc tggtggtggt ggttgagcac ggtaaaggcg
    32761 acatgagtct ttacggctat aatcagagcg cactggtgag cgttggttcg caggttcgcg
    32821 cgggccagcc aattgcactg gtgggcagca gtggcggtca gggtcggcct tcactctatt
    32881 tcgaaattcg ccgccagggt caggcggtca atccacagcc gtggttggga agataagttt
    32941 tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct gttggcgctc tcctcccccg
    33001 tacttgctgg caaacttgcc atcgtcattg atgattttgg gtatcgcccg cacaacgaaa
    33061 accaggtgct ggcgatgcct tccgctatct ccgtcgctgt attacccgat tcaccgcacg
    33121 ccagagagat ggcgaccaaa gcgcataaca gcggtcacga agtgttgatt catctcccga
    33181 tggcaccgtt gagtaaacag ccgctggaga aaaatacgct acgcccggag atgagcagcg
    33241 acgaaattga gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc gtggggatca
    33301 acaaccacat gggcagcaag atgacctcta acctgtttgg tatgcagaaa gtgatgcagg
    33361 cgctggagcg ttacaatctt tacttcctcg acagcgtaac catcggtaat acccaggcga
    33421 tgcgcgccgc gcaaggtact ggcgtgaagg tgatcaaacg gaaggtgttc ctcgacgatt
    33481 cgcagaacga agcggacatc cgtgtgcaat ttaatcgcgc aattgatctg gcgcgtcgca
    33541 acggttcaac cattgccatt gggcatcctc acccttcaac ggtacgcgtg ctacaacaga
    33601 tggtttataa cctgccgcca gacattacgc tggtgaaagc cagcagcttg ctgaatgaac
    33661 cgcaggttga tacttctaca ccgccgaaaa acgctgtgcc tgatgcaccg cgtaatccgt
    33721 tccgtggcgt gaagctgtgc aaaccgaaga aaccgataga acctgtttat gctaatcgct
    33781 tctttgaagt attaagcgaa agcatcagcc agagcacgct gatcgtttac ttccagcatc
    33841 agtggcaagg ctggggcaaa cagcccgaag cggcgaagtt taacgctagc gcaaattaaa
    33901 ggcgctacgc gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa
    33961 agaccacagt aacgcctggt aaccgacctt cacgctgcgc acattggtaa tcaggcgctt
    34021 atacataccc ggaagtaaat atctcggcaa tcatccgttg gcgggtaaga atatccggct
    34081 ctttgcgc
//
LOCUS       DAJVRP010000036        33543 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVRP010000036 DAJVRP010000000
VERSION     DAJVRP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.036"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OQN12_001772"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OQN12_001772"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1654133.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OQN12_001773"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OQN12_001773"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1654134.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OQN12_001774"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OQN12_001774"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS1654135.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OQN12_001775"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OQN12_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1654136.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OQN12_001776"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OQN12_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1654137.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OQN12_001777"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OQN12_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCS1654138.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OQN12_001778"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OQN12_001778"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1654139.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OQN12_001779"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OQN12_001779"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1654140.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OQN12_001780"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OQN12_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1654141.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OQN12_001781"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OQN12_001781"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1654142.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OQN12_001782"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OQN12_001782"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1654143.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OQN12_001783"
     CDS             8212..9075
                     /locus_tag="OQN12_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1654144.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OQN12_001784"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OQN12_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1654145.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OQN12_001785"
     CDS             10411..11028
                     /locus_tag="OQN12_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1654146.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OQN12_001786"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OQN12_001786"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1654147.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OQN12_001787"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OQN12_001787"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1654148.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OQN12_001788"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OQN12_001788"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1654149.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OQN12_001789"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OQN12_001789"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1654150.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OQN12_001790"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OQN12_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1654151.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OQN12_001791"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OQN12_001791"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1654152.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OQN12_001792"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OQN12_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1654153.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OQN12_001793"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OQN12_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1654154.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OQN12_001794"
     CDS             21859..23568
                     /locus_tag="OQN12_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1654155.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OQN12_001795"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OQN12_001795"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1654156.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OQN12_001796"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OQN12_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OQN12_001797"
     tRNA            27625..27719
                     /locus_tag="OQN12_001797"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28020..29204
                     /locus_tag="OQN12_001798"
     CDS             28020..29204
                     /locus_tag="OQN12_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1654157.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29616..30659
                     /locus_tag="OQN12_001799"
     CDS             29616..30659
                     /locus_tag="OQN12_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS1654158.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31174..31602
                     /locus_tag="OQN12_001800"
                     /pseudo
     CDS             31174..31602
                     /locus_tag="OQN12_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31632..32783)
                     /locus_tag="OQN12_001801"
                     /pseudo
     CDS             complement(31632..32783)
                     /locus_tag="OQN12_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32848..33502
                     /locus_tag="OQN12_001802"
                     /pseudo
     CDS             32848..33502
                     /locus_tag="OQN12_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8403 a         8816 c         8267 g         8057 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcatgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaaagccg gaggatgtca
    28021 tggcattaac cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caagggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt
    29101 ccatcagggg tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat
    29161 ggtacgctga ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc
    29221 cctgatgttt tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg
    29281 ttattatatt atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt
    29341 gacattttta aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca
    29401 aaggaatagt tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa
    29461 tgagattcac ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag
    29521 caatattcat tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc
    29581 agactatatc accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag
    29641 tgcacgataa caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg
    29701 agtttgaaga agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca
    29761 acaaactccg acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg
    29821 catatgcatt tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac
    29881 tggcggcagg taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc
    29941 ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac
    30001 agcgccatca ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaaaata
    30061 atcagggggc tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag
    30121 ccttcagtgt actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc
    30181 tcctgcattt tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg
    30241 ccacaaacac ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg
    30301 tgtggcatga cttctccggc tctctggata cggcagaaga ttactatgca acatactcac
    30361 cattatgggg acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct
    30421 ccgtgctgca tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg
    30481 atttcgaaat atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca
    30541 agacactttc tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc
    30601 cctggatatt gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga
    30661 tattgcatta gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt
    30721 tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag
    30781 cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt
    30841 tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa
    30901 tcccctcctc tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat
    30961 aaacagtaaa aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg
    31021 tgcttgtgtt ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat
    31081 tgcatccaac ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa
    31141 atcaatggat ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc
    31201 tcatactggc gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa
    31261 gacgcccaaa atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt
    31321 gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc
    31381 ccaaaagttt atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat
    31441 attgttacac cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg
    31501 ccagcgacct ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt
    31561 ggtgagattg ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg
    31621 tggacacggc cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag
    31681 gcactgtgac gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc
    31741 attatttccc ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag
    31801 aagctttcca cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg
    31861 ttgtgtcgct tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta
    31921 tgaacaatga cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag
    31981 gccagttgtg ctgtcattcg cggtgacatc gaccagccaa tgacggagcg tgaccacagg
    32041 tcgatgacta ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc
    32101 gcccacttct ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac
    32161 gggcaggcca tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag
    32221 cccctgacga cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc
    32281 aaggcgggga gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc
    32341 gcaggtgagc cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata
    32401 ccagccgctg cgggcaactc gaagtacacg acacatcgct ttgatgctga actctgcccg
    32461 atgattttcg ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt
    32521 tggaggatgg acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca
    32581 gcggccagtt cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag
    32641 gcataaagct gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt
    32701 tcagcgagtt tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg
    32761 ctgattgata ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct
    32821 gtgtccacta ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat
    32881 atatcatctg taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt
    32941 gaatattata actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg
    33001 ttattatcac caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc
    33061 tttgtgacca aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta
    33121 ttttgctaca tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt
    33181 agttactcaa ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca
    33241 aagattgaaa aattatccaa cctatggttt ttattaccca atagccaatt ttatcgcatt
    33301 acaaataaat ggttcaaaaa caggatttgc cgcatctatt acaggtttta ctcaaacaat
    33361 aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc gacaacggtg ttggtcccgg
    33421 aactacttta ggtcttggtt cactaataac ttcattactg tcaatagccg taatcgccat
    33481 tgcaggaaat aataaaaagt aagtgtactc tgactgcgta tgtatctact tgaaccgccc
    33541 cgg
//
LOCUS       DAJVRP010000037        33169 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVRP010000037 DAJVRP010000000
VERSION     DAJVRP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.037"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(41..133)
                     /locus_tag="OQN12_001803"
     tRNA            complement(41..133)
                     /locus_tag="OQN12_001803"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Ser,seq:gct)
     gene            complement(449..634)
                     /gene="csrA"
                     /locus_tag="OQN12_001804"
     CDS             complement(449..634)
                     /gene="csrA"
                     /locus_tag="OQN12_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1654159.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(869..3499)
                     /gene="alaS"
                     /locus_tag="OQN12_001805"
     CDS             complement(869..3499)
                     /gene="alaS"
                     /locus_tag="OQN12_001805"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1654160.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3627..4127)
                     /gene="recX"
                     /locus_tag="OQN12_001806"
     CDS             complement(3627..4127)
                     /gene="recX"
                     /locus_tag="OQN12_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1654161.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4370..5431)
                     /gene="recA"
                     /locus_tag="OQN12_001807"
     CDS             complement(4370..5431)
                     /gene="recA"
                     /locus_tag="OQN12_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1654162.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5511..6008)
                     /gene="pncC"
                     /locus_tag="OQN12_001808"
     CDS             complement(5511..6008)
                     /gene="pncC"
                     /locus_tag="OQN12_001808"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1654163.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6153..7238)
                     /gene="mltB"
                     /locus_tag="OQN12_001809"
     CDS             complement(6153..7238)
                     /gene="mltB"
                     /locus_tag="OQN12_001809"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1654164.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7494..8056
                     /gene="srlA"
                     /locus_tag="OQN12_001810"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7494..8056
                     /gene="srlA"
                     /locus_tag="OQN12_001810"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8053..9012
                     /gene="srlE"
                     /locus_tag="OQN12_001811"
                     /gene_synonym="gutE"
     CDS             8053..9012
                     /gene="srlE"
                     /locus_tag="OQN12_001811"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1654165.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            9023..9394
                     /gene="srlB"
                     /locus_tag="OQN12_001812"
                     /gene_synonym="gutB"
     CDS             9023..9394
                     /gene="srlB"
                     /locus_tag="OQN12_001812"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1654166.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9398..10177
                     /gene="srlD"
                     /locus_tag="OQN12_001813"
     CDS             9398..10177
                     /gene="srlD"
                     /locus_tag="OQN12_001813"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCS1654167.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10282..10641
                     /gene="gutM"
                     /locus_tag="OQN12_001814"
                     /pseudo
     CDS             10282..10641
                     /gene="gutM"
                     /locus_tag="OQN12_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10708..11481
                     /gene="srlR"
                     /locus_tag="OQN12_001815"
                     /gene_synonym="gutR"
     CDS             10708..11481
                     /gene="srlR"
                     /locus_tag="OQN12_001815"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1654168.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11474..12439
                     /gene="gutQ"
                     /locus_tag="OQN12_001816"
     CDS             11474..12439
                     /gene="gutQ"
                     /locus_tag="OQN12_001816"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1654169.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12436..13950)
                     /gene="norR"
                     /locus_tag="OQN12_001817"
     CDS             complement(12436..13950)
                     /gene="norR"
                     /locus_tag="OQN12_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1654170.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14137..15576
                     /gene="norV"
                     /locus_tag="OQN12_001818"
     CDS             14137..15576
                     /gene="norV"
                     /locus_tag="OQN12_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1654171.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15573..16706
                     /gene="norW"
                     /locus_tag="OQN12_001819"
     CDS             15573..16706
                     /gene="norW"
                     /locus_tag="OQN12_001819"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCS1654172.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16834..19086)
                     /gene="hypF"
                     /locus_tag="OQN12_001820"
     CDS             complement(16834..19086)
                     /gene="hypF"
                     /locus_tag="OQN12_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1654173.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19239..19766)
                     /gene="hydN"
                     /locus_tag="OQN12_001821"
     CDS             complement(19239..19766)
                     /gene="hydN"
                     /locus_tag="OQN12_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS1654174.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19915..20925)
                     /gene="ascG"
                     /locus_tag="OQN12_001822"
     CDS             complement(19915..20925)
                     /gene="ascG"
                     /locus_tag="OQN12_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1654175.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20939..21058)
                     /locus_tag="OQN12_001823"
     CDS             complement(20939..21058)
                     /locus_tag="OQN12_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCS1654176.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21068..22777)
                     /gene="hycE"
                     /locus_tag="OQN12_001824"
     CDS             complement(21068..22777)
                     /gene="hycE"
                     /locus_tag="OQN12_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1654177.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22795..23718)
                     /gene="hycD"
                     /locus_tag="OQN12_001825"
     CDS             complement(22795..23718)
                     /gene="hycD"
                     /locus_tag="OQN12_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1654178.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23721..25520)
                     /gene="hycC"
                     /locus_tag="OQN12_001826"
     CDS             complement(23721..25520)
                     /gene="hycC"
                     /locus_tag="OQN12_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCS1654179.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGA
                     GSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKL
                     SNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMS
                     VSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFII
                     MAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSL
                     VPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25557..26168)
                     /gene="hycB"
                     /locus_tag="OQN12_001827"
     CDS             complement(25557..26168)
                     /gene="hycB"
                     /locus_tag="OQN12_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS1654180.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26293..26754)
                     /gene="hycA"
                     /locus_tag="OQN12_001828"
     CDS             complement(26293..26754)
                     /gene="hycA"
                     /locus_tag="OQN12_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS1654181.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26966..27316
                     /gene="hypA"
                     /locus_tag="OQN12_001829"
     CDS             26966..27316
                     /gene="hypA"
                     /locus_tag="OQN12_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1654182.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27320..28192
                     /gene="hypB"
                     /locus_tag="OQN12_001830"
     CDS             27320..28192
                     /gene="hypB"
                     /locus_tag="OQN12_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1654183.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28183..28455
                     /gene="hypC"
                     /locus_tag="OQN12_001831"
     CDS             28183..28455
                     /gene="hypC"
                     /locus_tag="OQN12_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1654184.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28455..29576
                     /gene="hypD"
                     /locus_tag="OQN12_001832"
     CDS             28455..29576
                     /gene="hypD"
                     /locus_tag="OQN12_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1654185.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29573..30583
                     /gene="hypE"
                     /locus_tag="OQN12_001833"
     CDS             29573..30583
                     /gene="hypE"
                     /locus_tag="OQN12_001833"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1654186.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30657..32735
                     /gene="flhA"
                     /locus_tag="OQN12_001834"
     CDS             30657..32735
                     /gene="flhA"
                     /locus_tag="OQN12_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCS1654187.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32772..33125)
                     /locus_tag="OQN12_001835"
     CDS             complement(32772..33125)
                     /locus_tag="OQN12_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1654188.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7817 a         9243 c         8631 g         7478 t
ORIGIN      
        1 cgtagccgag tactctatcc agctgagcta cggatgcaaa tggcggtgag gcggggattc
       61 gaaccccgga tgcagctttt gaccgcatac tcccttagca ggggagcgcc ttcagcctct
      121 cggccacctc accacacgcc tcttacgagt gcttcgaaga acttgtttat cgctcatcat
      181 cgctgcgtgg cgcacatatt actttctgag acttataagt caaacaattt ttcccacact
      241 tttatcgttt gcacacttca cgctcaatta gtctgtaaaa gcgtcaaaaa gagtgtttta
      301 tctacagaaa aacggaggtc tggcagatag cagtaatgca aaaaaatgga gacttaagtt
      361 gaatgaacgg gagtaaagcg aaaagactat agagtgaagg agaaattttg agggtgcgtc
      421 tcaccgataa agatgagacg cggaaagatt agtaactgga ctgctgggat ttttcagcct
      481 ggatacgctg gtagatctct tcacggtgaa cagaaacttc cttcggggca tttacgccaa
      541 tacgtacctg gttgcccttt acccctaaaa ctgtcacggt gacctcatcc ccaatcatga
      601 gggtctcacc aactcgacga gtcagaatca gcattctttg ctccttgaaa gattaaaaga
      661 gtcgggtctc tctgtatccc ggcattatcc atcatataac gccaaaaagt aagcaatgac
      721 aaacacatta catgtaagca gtcatggcat tacattctgt taaacctaag tttagccgat
      781 atacacaact tcaacctgac tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc
      841 gagtccacgg cattgcctga cgcttatatt attgcaattt cgcgctgacc cagcctttca
      901 cactggctaa cgctgcaggt aaggccgcag catccgtacc accggcttgc gccatgtcag
      961 gacgtccacc acccttgccg cccacctgct gagcgaccat accaatcagt tcccctgctt
     1021 tcacacgatc tgtgacgtcc ttagatacgc ctgcaatcag agaaacctta ccttcggcta
     1081 ccgttgccag cacgataatt gtcgacccca gctgattttt taaatcgtca accatggtac
     1141 gcaacatttt cggctcaaca ccgctaagct cgctaaccaa cagcttaaca ccattaacat
     1201 caattgcctt actggaaaga tttgcgctct cctgtgcggc agcttgttct ttaagctgtt
     1261 gtagttcttt ttctaactga cgcgtacgtt ccagtactga gcgcacttta tccgccagat
     1321 tattgctatc gcctttcagc agatgcgcga cttcgcttaa gcgatcgctg tctgcatgaa
     1381 cggtggtgat agcgccttct ccggttaccg cttcgatacg acgaacgcct gcagcagtac
     1441 ccgattcaga gatgatgcgg aacagaccaa tatcaccagt gcggctggcg tgagtaccgc
     1501 cacacagctc ggtggagaaa tcgcccatgc tcagcacgcg tacacgctca tcatacttct
     1561 cgccgaacag cgccatcgca cctttcgctt tcgccgcttc gagattcatg atgttggttt
     1621 cgatcggcaa attgcgacga atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt
     1681 ccggtttcat cgcttcgttg tgtgagaagt cgaagcgcag caccttgtcg ttaaccagtg
     1741 aacctttctg cgatacatga gtccccagaa cctggcgcag cgcagcgtgc atcaggtgcg
     1801 ttgcggagtg attcagacga atacgggcgc gacgagcctc atcaatatca gcctgcacag
     1861 cgtcgcccac tttcagagaa cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt
     1921 atttctgcgt atcttccacc gcgaaggaga agttagcgcc tttcagttcg cctttatcac
     1981 caacctgacc gccggattcc gcatagaatg gcgtttgatc cagcacgacc acagcttcct
     2041 ggcctgcatt gatggcatca accgctttac catcaacaaa cagcgcggtc actttgccgt
     2101 tcagttccag atggtcatag cctttaaatt cagatgcact atcaacacgg atcattgcgt
     2161 tgtaatcggc accaaagccg ctggcttcgc gcgcgcgacg acgctgctct tccattgctg
     2221 cttcaaaacc agcttcgtca actttgatgt tgcgctcacg acaaacatca gccgtcaggt
     2281 caaccgggaa gccataggtg tcgtacagac ggaaagccgt ttcaccatcc agcgtatcac
     2341 cagaaagttt tgccagctct tcatccagca acgccagacc gcgctccaga gtacgggcaa
     2401 actgctcttc ttcagtcttc agcacctgct caacctgcgc ctgctggcgt ttcaggtctt
     2461 cacccgcaga gcccataacg tcgatcagcg gaccaaccag tttgtagaag aaggtttctt
     2521 tcgcgccgag catattgccg tgacgcactg cgcgacgaat gatacgacgc agtacataac
     2581 cacggttttc attggacggc attacgccat ccgcgatcag gaacgcacaa gaacgaatgt
     2641 ggtcagcgat tacgcgcagc gatttattgc tcagatcggt cgcgccagtg actttcgcta
     2701 ccgcctggat caacgtgcgg aacaggtcga tgtcatagtt agagttaacg tgttgcagca
     2761 ccgcagcaat acgctccaga cccataccgg tatctacaga cggcttcggc agcggttcca
     2821 tcgtgccatc tgcctggcgg ttgaactgca tgaagacgat gttccagatc tcaatgtagc
     2881 ggtcgccgtc ttcttccgga cttcccggag ggccacccca aatgtggtcg ccgtgatcgt
     2941 agaagatttc ggtgcacggg ccgcacggac cagtgtcacc catctgccag aagttgtcag
     3001 atgcgtatgg cgcacctttg ttatcgccga tgcgaataat acgttcgcgc gggatcccga
     3061 cttctttttc ccagatttcg taggcttcgt cgtcgctttc atagacggta acccacagac
     3121 gctctttcgg cagggcaaac catttttcgc tggtcagcag ttcccatgca aactgaatgg
     3181 catcgtgttt gaaatagtcg ccgaagctga agttgcccag catttcgaag aaggtatggt
     3241 gacgcgcggt gtaaccgacg ttttccaggt cgttgtgttt accacccgca cgcacgcagc
     3301 gttgggaagt ggtagcgcgg gaataattac gcttgtcgag cccaaggaac acatccttga
     3361 actggttcat cccggcgttg gtaaacaaca aagttgggtc gttatggggt accagggagc
     3421 tgctggcaac tacctgatgt cccttactat ggaaaaagtc gagaaacgcc tgacggatct
     3481 cagcggtgct cttgctcata attatcctga aatcaagcta acgaaatatc gccaccagct
     3541 ccagcgtgtc ttaaccgccg ggctggtaac tgaaaagtgg gaataagata agttttcttg
     3601 actgggaagt aaaataccgt atgcgttcag tcggcaaaat ttcgccaaat atcctggata
     3661 tcttccatca gatagccacg atagagcaga aaacgctgga tcttaacttt ttctgaaaag
     3721 acagttggca aaggttcacc atattttcgc gtcgcctgat cgcgcgccag tgcgcaccag
     3781 tcgatgtcac attcacgcat cgctttttct gtcgcttcgc gggaaatacc tttctgattc
     3841 agttcctgac gaatacgtgc aggtccatac cctttgcggc tacggctggc gataaagcgc
     3901 gcaacaaatc ggctgtcatc gagatagcca tgttcatggc accaggcaat aacgcgctcg
     3961 taatcttctg ccgtagcatc aatctcttct gggccatttt tgcccataat cggtgccgcg
     4021 agtttacgtc gcagttcttg ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga
     4081 tccaacaggc gagcatatgc cgggcgacgg gatgttgatt ctgtcatggc atatccttac
     4141 aacttaaaaa agcaaaaggg ccgcagatgc gccccaggtt ttgctgataa acagatttaa
     4201 tagtcaatga gtatgcagaa tgttcccgca ggcccaattc gttagaggat cgaggtgaga
     4261 gtaatttttc acgccagtgc tggcctgttc ccctcaccct aaccctctcc ccaaaggggc
     4321 gaggggaccg tccgtgcaca cattatctat tatcatcaat aacgaacgat taaaaatctt
     4381 cgttagtttc tgctactcct tcgctgtcat ctacagagaa atccggcgtt gagttcgggt
     4441 tgctcagcag caactcacgt actttcttct cgatctcttt cgcggtttcc gggttatctt
     4501 tcagccaggc agttgcattc gctttaccct gaccgatctt ctcacctttg tagctgtacc
     4561 acgcgcctgc tttctcgatc agcttctctt ttacgcccag gtcaaccagt tcgccgtaga
     4621 agttgatacc ttcgccgtag aggatctgaa attcagcctg tttaaacggt gcagcgattt
     4681 tgttcttcac cactttcacg cgggtttcgc tacccaccac gttttcgccc tctttcaccg
     4741 cgccgatacg acggatgtcg agacgaacag aggcgtagaa tttcagcgcg ttaccaccgg
     4801 tagtggtttc cgggttaccg aacatcacac caattttcat acggatctgg ttgatgaaga
     4861 tcagcagcgt gttggactgc ttcaggttac ccgccagctt acgcatcgcc tggctcatca
     4921 tacgtgccgc aaggcccatg tgagagtcgc cgatttcgcc ttcgatttcc gctttcggcg
     4981 tcagtgccgc cacggagtca acgacgataa cgtctactgc gccagaacgc gccagggcgt
     5041 cacagatttc cagtgcctgc tcgccggtat ccggctggga gcacagcagg ttatcgatat
     5101 cgacgcccag tttacgtgcg tagattgggt ccagcgcgtg ttcagcatcg ataaacgcac
     5161 aggttttacc ttcacgctgc gctgcggcga tcacctgcag cgtcagcgtg gttttaccgg
     5221 aagattccgg tccgtagatt tcgacgatac ggcccatcgg cagaccacct gccccaagcg
     5281 cgatatccag tgaaagcgaa ccggtagaga tggtttccac atccatggaa cggtcttcac
     5341 ccaggcgcat gatggagcct ttaccaaatt gtttctcaat ctggcccagt gctgccgcca
     5401 acgctttctg tttgttttcg tcgatagcca tttttactcc tgtcatgccg ggtaataccg
     5461 gatagtcaat atgttctgtt gaaacaatta tactgtatgc tcatacagta tcaagtgttt
     5521 tgtagaaatt gttgccacaa ggtctgcaat gcatacgcag tagcctgacg acgcaccgca
     5581 tcacggtcgc cgctgaagca ttcccgccgg gtaatgcctt caccgcgggc agtggcaaaa
     5641 gcaaaccaga cggtgccgac aggcttctct tcactgccgc catccggccc ggcgatacca
     5701 ctaatagaca cggcataatc agcacgagcc gctttcagtg cacctatcgc catttccacc
     5761 acgacgggtt cactcaccgc gccatgctgc gccagcgtct cttcgcgtac gccgatcatc
     5821 tgcgctttgg cttcgttact gtaggtgaca aatccgcgtt caaaccaggc ggagctaccg
     5881 gcaatatcgg taatcacttt cgctacccaa ccaccggtac aagactcggc agttgttacg
     5941 gttgcgccac gggctttcag cgcctgccca acctgttcgc ttaactgcat cagttcactg
     6001 tcagtcatca caacctctgt cagtcaaaaa tttatgccgg acaagatagc actttcgtca
     6061 ggaagatggt gttggcgttg caattttacg aggaggattc agaaaaaagc tgattagcca
     6121 gagggaagct cacgcccccc tcttgtaaat agctactgta ctcgcgccag cgccacggct
     6181 tgtcctaact gccagaccgc cattgcgtaa tgggtgctgt ggttgtaacg ggtgatggtg
     6241 tagaagttcg gcagaccgta ccagtactgg tagccggtgc caacatccag acgcagcagg
     6301 ctggcttgtt gatggttgcc cagcggctgc tgtggcgtta aacctgcggc ggcaagctgc
     6361 gaaatgctgt acttagtttt gaaaccattt ggcaagcctg gagcctgacc gtttgccatt
     6421 atcgcgacct gatcgccttt cacccagccg tgcgctttga aatagttcgc cacgctaccg
     6481 atcgcatcaa ccggatccca caggttgata tgcccgtcgc cgctgaaatc taccgcatat
     6541 tgtttgtaag acgacggcat aaactgtccg tagcccatcg ccccggcaaa ggaacctttc
     6601 agattgagcg gatcgtcctg ctcgtcgcgc gccatcagca ggaaggtttc cagctcgcca
     6661 gagaaatact ccgcgcggcg tgggtagtta aatgacagcg ttgccagcgc atcgaggatg
     6721 cgagttttcc ccatcacgcg cccccagcgg gtttcaacgc cgataatccc gacgataatt
     6781 tccggcggta cgccatacac ctgccacgcg cgattcaacg catcttcata ctgattccag
     6841 aaaaccacac cgttctgcac gttgtccggc gtaataaatt ttttgcgata acgcagccat
     6901 gcgccgttcg gacctgatgg cggtttcacc gatgtggttg gtgcctggtt atccatcagc
     6961 cgcagtaccg aatccagacg cttcgcctgg gagagaattt cctgcaactg ctgacgatcg
     7021 aaaccgtgtt tgttcaccat tttgtcgatg aactgctggg cattcgggtt attagcgaaa
     7081 tcgccgccca tctgcatcac attgtgctgc ggctcaagca ggaagccgcc agacggcgtt
     7141 ccggtggtcg tttcagtctc agtaggtttt ggcttgctgc tacaggcggc aagcaacaca
     7201 aaaaggggaa gcaatgttac ataacgacgc ttgaacatga ggggtccatt taacagattc
     7261 aaccaggggc aagtatggta aagcatcaca ccccgcacaa ggaagcggta gtcactgccc
     7321 gatacggact ttacataact caactcattc ccctcgctat acttttattc aaactttcaa
     7381 attaaaatat ttatctttca ttttacgatc aaaataacac ttttaaacct ttcaacctga
     7441 ttagattagg ttgccgtttg gtaataaaac aataaatcct gaaggagaga acaatgatag
     7501 aaaccattac tcatggtgca gagtggttta tcgggctgtt ccaaaagggc ggagaggtgt
     7561 ttaccgggat ggtgaccggc attcttccgc tgttgattag cctgcaggtt atcatgaacg
     7621 cactgattaa ttttatcggt cagcatcgta ttgaacgttt tgctcaacgt tgcgccggta
     7681 accctgtttc ccgttaccta ctgttaccgt gcattggcac gtttgtcttt tgcaatccga
     7741 tgaccttaag cctgggtcgc tttatgccgg aaaagtacaa acccagctac tacgcggcgg
     7801 cctcttatag ctgccactca atgaatggcc tcttccccca tatcaaccct gcgaactgtt
     7861 tgtttatctt ggcattgcca gcggtctgac aacgctgaac ctgccacttg gcccactggc
     7921 ggtgagttat ctgctggttg gtctggtcac caatttcttc cgcggctggg tgaccgatct
     7981 gaccaccgcc atttttgaga aaaagatggg cattcaactt gaacaaaaag ttcacctggc
     8041 aggagcaaca tcatgacgcg tattcggatc gaaaaaggaa cgggtggctg gggcggcccg
     8101 cttgagctgg aagccacgcc gggcaaaaaa atcgtctata tcaccgccgg tacccggcct
     8161 gcgattgttg acaaactggc acaacttact ggctggcagg ctattgacgg atttaaagaa
     8221 ggtgaacccg cggaggcgga aattggtgtc gcggtaatcg actgtggcgg cacattacgc
     8281 tgcggcatct atccgaaacg acgtattccc accattaata tccactcgac gggcaagtcc
     8341 ggtccgctgg cgcagtacat tgtggaagat atttatgtct ctggcgtaaa agaagaaaac
     8401 atcactgtag tgggtgatgc gacaccacaa ccctcttccg tgggccgtga ctatgacacc
     8461 agtaagaaaa tcaccgaaca aagcgatggt ttactggcga aggtgggaat gggcatgggg
     8521 tccgccgttg ccgtgttgtt tcagtctggt cgtgacacca tcgacactgt attaaaaacc
     8581 attctgccgt ttatggcatt cgtctcggcg ctcattggca tcattatggc ttctggcctt
     8641 ggtgactgga ttgcccacgg tcttgctccg ctagcgagcc atccactggg tctggtcatg
     8701 ctagcgctca tctgctcctt cccactgctt tcacctttcc tcggcccagg cgcagttatc
     8761 gcacaggtta tcggcgtatt gattggcgtg cagattggtc tcggcaatat tccgccgcat
     8821 ctggctttac cggcactgtt tgccatcaac gcgcaggcgg cttgcgactt catcccggtc
     8881 ggtttgtcgc tggcggaagc ccgtcaggac acggttcgcg tcggtgtccc ttctgtactg
     8941 gtgagccgct ttttaaccgg cgcgccgact gtactgatcg cctggtttgt ctccagtttt
     9001 atctatcaat agaggctgaa acatgaccgt tatttatcag accaccatca cccgtatcgg
     9061 cgcgagtgcc actgacgccc tcagcgacca gatgctcatc acctttcgtg aaggcgcgcc
     9121 agcggacctc gaagagtatt gcttcattca ttgccacggc gagttgaaag gtgcactcca
     9181 tcccggtttg caattttcac tcgggcagca tcgctatccg gtgaccgctg ttggcagcgt
     9241 ggcggaagac aaccttcgcg aactgggtca tgtcaccctg cgcttcgatg gtttaaacga
     9301 agcggaattt ccgggcactg tccatgtggc aggccctgta cccgacgata tcgcgccggg
     9361 atcggttttg aagtttgaat ctgttaagga gtaaaaaatg aatcaggttg ccgttgtcat
     9421 cggtggtggg caaaccttag gcgcgttcct gtgccacggt ctggctgccg aggggtatcg
     9481 cgtcgcggtt gtcgatattc agagcgacaa agccgcaaat gtggcacaag aaattaacgc
     9541 cgaatatggt gaaggtatgg cgtacggttt tggtgctgac gccactagcg agcaaagcgt
     9601 tctggcgctc tctcgtgggg tagatgaaat ctttggtcgc gtggatttgc tggtctatag
     9661 cgccggaata gcaaaagcag cctttatcag cgacttccag ctcggcgatt ttgaccgttc
     9721 gctacaagtg aatctggtgg gttatttcct gtgtgcccgt gaattttcgc gtttgatgat
     9781 ccgcgacggg attcagggtc gcattattca gatcaactcg aaatccggca aagtgggcag
     9841 caaacacaac tctggctaca gcgcagcgaa atttggtggc gtcgggctga ctcaatcact
     9901 ggcgctggat ctggcggagt acggcattac ggtacattca ctgatgctcg gtaacctgct
     9961 gaaatcgcgg atgttccagt cactgttgcc acaatacgcg accaagctgg gtatcaaacc
    10021 ggatcaagtc gagcagtatt acatcgacaa agtaccgctc aaacgcggct gcgattatca
    10081 agatgtgctg aatatgctgc tgttctacgc cagtcctaag gcgtcgtact gcaccggaca
    10141 gtcgatcaat gtcaccggcg gtcaggtgat gttctgatca acagcggaga tccgttaagg
    10201 atctccgtga gactatagaa tgcctgatgc gctacgctca tcaggcatac aggacttccg
    10261 ccactacatt aaggaaaagt tatggtatcc gcactcatca ccgtcgccgt tatcgcctag
    10321 tgtgcgcaac tggccttagg cggctggcaa atttctcgtt ttaaccgtgc cttcgacaca
    10381 ctatgccagc aagggcgggt tggcgtgggc cgttccagcg ggcgctttaa accgcgggtc
    10441 gtggtcgcca tcgcgctgga cgatcagcag tgcatcgtcg acaccttgtt tatgaaagga
    10501 ctgactgtct tcgcccgacc gcaaaaaatt cccgcaatta ccggcatgca tgcgggtgat
    10561 ttacagcccg atgtgatctt tccccatgat ccactatcac agaatgctct atcattggcg
    10621 cttaaactga aacgtggata atttcgttgt gaatgttact tgcttgcgaa gttatcattt
    10681 tgaaacctaa atcaggtaat cacgcccatg aaacctcgtc agcgtcaggc cgccattctg
    10741 gagtatctgc aaaagcaggg taaatgctcg gttgaagaat tggcgcaata ctttgacacc
    10801 acaggcacaa ccattcgcaa agatctggtc attctggaac atgccggaac cgtcattcgt
    10861 acttatggcg gagtggtgtt gaataaagag gaatccgatc cgcctatcga tcataaaacg
    10921 ctcatcaaca cccacaagaa agagctgatt gcagaagctg ccgttagttt tatccatgat
    10981 ggcgattcga tcattcttga tgctggcagt accgttttgc agatggttcc cctgctctcg
    11041 cgctttaata acatcacggt gatgaccaac agcctgcata tcgtcaatgc gctatccgaa
    11101 ctggataacg aacaaactat cctgatgcca ggcggaacat ttcgcaaaaa atcggcctca
    11161 tttcacgggc aactggcaga gaatgccttc gagcatttca ccttcgataa attgtttatg
    11221 ggcaccgacg gcatcgatct caatgcgggc gtaaccacgt tcaacgaggt ttataccgtc
    11281 agtaaggcaa tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc atcaaagttt
    11341 ggccgtaaaa gccccaacgt agtttgcagt cttgaaagcg tcgataagct aattaccgac
    11401 gcaggtatcg atccggcgtt tcgtcaggcg ctggaagaga aagggatcga tgtgatcata
    11461 accggagaga gcaatgagtg aagcactact gaacacggga cgtcagacgt taatgctgga
    11521 gttgcaggaa gcaagccgtt taccggaacg tctgggcgat gattttgttc gcgccgccaa
    11581 tatcatcctg cactgcgaag gcaaagtggt ggtttcggga attggcaaat cgggccacat
    11641 tggtaagaaa atcgccgcaa cacttgccag taccggcaca ccggcttttt ttgtccatcc
    11701 ggcagaagca ctgcacggcg atctggggat gattgaaagc cgcgatgtga tgctgtttat
    11761 ctcttactcc ggtggcgcga aggaactgga tctgattatt ccgcgtctgg aagataaatc
    11821 tatcgcgctg ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg cggcgaaagc
    11881 ggtgctggat atctccgtag aacgcgaagc ctgcccgatg caccttgcgc cgacctccag
    11941 caccgtcaat accctgatga tgggtgacgc gctggcgatg gcggtcatgc aggcgcgcgg
    12001 ttttaatgaa gaagattttg cccgctccca cccagctggg gcactgggcg ctcgcttgct
    12061 gaataaagtg catcatctga tgcgtcgtga tgacgctatc ccacaggtag cgttaaccgc
    12121 cagcgtgatg gatgcgatgc tggaactcag ccgcaccggt ctggggctgg tggcggtatg
    12181 tgacgctcaa caacaggtac aaggcgtctt taccgacggc gatttacgtc gctggctggt
    12241 aggcggcggc gcactcacca cgccagttaa tgaagcgatg acgacaggcg gcaccacatt
    12301 acaggcacaa agtcgcgcca tcgacgccaa agaagttctg atgaaacgca aaatcactgc
    12361 cgcaccagtg gtagatgaaa acggcaaact caccggcgca attaacctgc aggatttcta
    12421 tcaggccggg attatttaat ccttcaatcc cagacgtttc gccagccgat gcaggttggc
    12481 gacgtcagtt tccagcattc gcgcacaggc tgcccagttg tgatgatttt gtgccagtgc
    12541 ctggcgaata gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt gcttaacaac
    12601 gagcaccgcc gccgcttctg gcggcggcaa cgtcacctca ggaaaagcaa aatgttgcgc
    12661 ctcaagaatc acttcatcgc cgttgcgggt ggctctcgcc agcactaccg cccgatgaat
    12721 agcatgttcc agttcgcgca cgtttcccgg aaaactgtag tgttgcagta aatttcgcgc
    12781 cccggcactt aataccacgc gggagagccc ctgccgcaaa cgacactgct cgcagaaata
    12841 ccccgccagc agaatgacat catcgccccg ctcacgcagc ggcggcaccg aaagtggaaa
    12901 cacgctcagg cgatgaaaca aatcggcgcg gaatcgccct gccagcacct cttcgcgtaa
    12961 gtcgcggtta gtcgccgcca gcacgcgcac atcgacccgc aaactacggt catcgccaac
    13021 gcgctgaata tcgccatact gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa
    13081 ctcgccgatc tcatcgagaa acagagtacc gttatccgcc atttcaaact tcccgctgcg
    13141 gttactgata gcgccagtaa acgctccttt cacatgcccg aacaactcac tttccgccac
    13201 actttccggc agtgcggcac agttgagata gaccagcgga ttcaccgccc gtggcgaggc
    13261 ttcatgaatc gctttcgcca ccagctcctt accggttccc gtctcaccgc tgatcagaac
    13321 gttgagatcg gacgccgcca caatctcaat ctcttttttc agttgcgtca tgccagggga
    13381 caagccaatc atctgcgtct gtttcaccgc ttcaaacggc gtggcatcgc ctggcagcat
    13441 attctggctt tccagttgtt caatcagcaa cgcattgctt aacgctcccg ccgccagcgc
    13501 ggcaatcagc cgtaactctt cgtcgctgaa aacatcgaac tgatcgggct gcatcccgtc
    13561 gagcgtcagt gcgccgatca ggttttgtcc gacaaacaat ggcagaccaa cgcaggcgtg
    13621 aaccttcaga ctctcctgcc caggaatcaa accgtcatag ggatcgggca attcgctgtc
    13681 tgcgggaaag cgcaccacat ccccggcgcg ggcaatcgct tccagccgtg gatgcccttc
    13741 cagcgcaaag cgtctaccga gtacatcctt tgccagaccg tcgatggcaa gcggaataaa
    13801 ctgccgcgaa tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct gacgtagcgt
    13861 ggtgatcagg cgctgaaaac gatcctgatg accaatccca cgctgcaatt cgatggcgat
    13921 attcgccagc acatcaacgg aaaaactcat ctttgcctca ctgtcaattt gactatagat
    13981 attgtcatat cgaccatttg attgatagtc attttgacta ctcattaatg agcataattt
    14041 tatttataga gtaaaaacaa tcagataaga aactggcacg caatctgcaa ttagcaagac
    14101 atctttttag aacacgctga ataaattgag gttgctatgt ctattgtggt gaaaaataac
    14161 attcattggg tgggtcaacg tgactgggaa gtgcgtgatt ttcacggcac ggaatataaa
    14221 acgctgcgcg gcagcagcta caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc
    14281 gacaccgtcg accataaatt cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat
    14341 ctggcggata tcgattacat cgtgattaac catgcagaag aggaccacgc cggggcgctg
    14401 accgaactga tggcgcaaat tcccgatacg ccgatctact gtactgccaa cgctatcgac
    14461 tcgataaatg gtcatcacca tcatccggag tggaatttta atgtggtgaa aactggcgac
    14521 acgctggata tcggcaacgg caaacagctc atttttgtcg aaacaccaat gctgcactgg
    14581 ccggacagca tgatgactta cctgacaggc gacgcggtgc tgttcagtaa cgatgctttc
    14641 ggtcagcact actgcgacga gcatctgttc aacgatgaag tggatcagac ggagcttttc
    14701 gagcagtgcc agcgttacta cgccaatatc ctgacgccgt tcagccgcct ggtaacgccg
    14761 aaaattaccg agatcctggg ctttaactta ccagtcgata tgatagcgac ctcccacggc
    14821 gtggtctggc gcgataaccc gacgcaaatt gtcgagctgt acctgaagtg ggccgctgat
    14881 tatcaggaag acagaatcac cattttctac gacaccatgt cgaataacac ccgcatgatg
    14941 gctgacgcta tcgcccaagg aattgcggaa accgacccac gcgtggcggt gaaaattttc
    15001 aacgtcgccc gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg
    15061 ctggtcggca cttcgacgat gaataacgtg atgatgccga aaatcgccgg gctggtggag
    15121 gagatgaccg gattacgctt ccgtaacaaa cgcgccagtg ctttcggatc tcacggctgg
    15181 agcggcggtg cgatggatcg cctttccacg cgcctgcagg atgcgggttt cgaaatgtcg
    15241 ctcagcctga aagccaaatg gcgaccagac caggacgctc tggagttatg ccgtgaacac
    15301 ggtcgcgaaa tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg
    15361 gtagttaaag aagaaacctc tgccacgacg acggctgacc tcggcccacg aatgcagtgc
    15421 agcgtctgcc agtggattta cgatccggca aaaggcgagc caatgcagga cgttgcgcca
    15481 ggaacgccgt ggagtgaagt cccggataac ttcctctgcc cggaatgctc cctcggcaaa
    15541 gacgtctttg acgaactggc atcggaggca aaatgagcaa cggcattgtg atcatcggtt
    15601 cgggcttcgc cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc actattccat
    15661 taaccctgat tgccgccgac agcatagatg agtacaacaa acccgacctc agccatgtta
    15721 tcagtcaggg gcaacgtgcc gacgacctta cccgccagac ggcgggtgaa tttgccgggc
    15781 agtttaatct gcgcctgttt ccgcacacct gggtgacgga tatcgatgcc gaagcccatg
    15841 tggtgaaaag ccagaataat cagtggcaat acgacaagtt agtgctggca accggtgcca
    15901 gcgcctttgt cccgcctgtg cctgggcgtg agttaatgct gacgttaaat agtcagcaag
    15961 agtatcgcgc ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt gtcggcggtg
    16021 gtttgattgg tagcgaactg gcgatggatt tttgtcgtgc aggcaaagcg gtcacgctaa
    16081 tcgacaacgc tgccagtatt ctggcgtcat taatgccacc agaagtaagc agccgcttgc
    16141 agcatcggtt gacggagatg ggcgttcatc tgctgttgaa atctcagttg caggggctgg
    16201 aaaaaacgga ttctggcatt ctggcaacgc tggaccacca gcgtagtatc gaagtggatg
    16261 cggtaattgc cgccaccgga ctgcgcccgg aaacagccct ggcacgacgc gccgggctga
    16321 cgattaatcg cggcgtttgc gtcgatagtt atctgcaaac cagtaatgcc gatatttacg
    16381 cgctgggcga ttgcgcggaa attaacggtc aggtattgcc gttcctgcag ccgattcaac
    16441 ttagcgggat ggtgctggca aaaaatcttc tcggcaataa cacgccgctg aaactaccgg
    16501 cgatgctggt gaaaatcaaa acgccggaat taccgctgca tctggcaggc gaaacccagc
    16561 gtcaggattt acgctggcag attaataccg aacgccaggg aatggtggcg cgcggcgttg
    16621 acgatgctga ccagcttcgc gcctttgtgg tcagtgagga tcggatgaaa gaggcatttg
    16681 tattgttgaa aacgttgccg gtgtaggtgg gctactgtgc ctaaaatgtc ggatgcgacg
    16741 ctggcgcgtc ttatccgacc tacggggacg catgtgtagg ccggataagg catttacgcc
    16801 gcatccggca agggtgtcca aatgcaacac gctttatccg ttctggactt cacccgctaa
    16861 ccaacgcgcc gcagcaataa ccccctgccc cagagacaaa ccgccatcac ccgccggtaa
    16921 actctgtgga aagagcaatg tgaaatcagc gagataatgc gccagacgtg cacgcagcaa
    16981 acggttatga ataaccccgc cgctaaatac cagcgtagtg ataccacgca tcgtggcctg
    17041 ctcacgcatc aacgcggcaa aaccctgcgc cagcgcatca tgaaacgccc acgcgcgttg
    17101 attaaccagt gcctgccagt tcagccactg ctgccagaaa gtggcgagat ccagttgatt
    17161 gtccacccgc ggcatcgtca ccggatgcgt cactccgtcg catgaggctg cgagcgcctc
    17221 cagagcacaa gccgcttcac cttcataact taacgtggct ggcgcacagc ccagtgccgc
    17281 cgccactgca tcgaacaacc gcccacacga tgatgccagc ggcgcgttaa ttccacgctc
    17341 aatggcccgc gccagtacgc tccagttttg ctgttgcaca cttgccgttt cagggtaatt
    17401 ctgccactcc ggcacaaagc gcaggcactg cgccagcagg tttcgccacg gctgcttcgc
    17461 cgccaaatcg ccacccggaa gcgccactgc aggcaagccg cccaggtgct cacattcgcg
    17521 atagttcacc cgcaggcact cgccgcccca caaagcgcca ttctccccca taccgatacc
    17581 gtcgagcgtc aaagcaatga catcaccgcc atccagcggc cactgatgct ctgccagaca
    17641 cgccgctgca tgggcatgat gatgcagtac cgtttgcgtc ggcagattca tttcgctcgc
    17701 ccactggctg gagacatagc ccggatgcac atcatgcaca atgtattgcg gagtgaaatc
    17761 gtagatgttt tgcatcaggc gtaacgcttc gcgccactgc atctggatgc catcgtcact
    17821 taaatcgccc agatgctgac tcaacaccgc ttgttcaccg cgcaccaggc agaaggtatt
    17881 tttcagatcc gcgccaagac acagcacagg cggaacattt ttaaagcccg gaggcaaaac
    17941 cagcgcatcc ggcacatacc cccgcgaacg gcgcagcatt tcgccgcttt cgcgcaccac
    18001 tgaatcatcc atccgctgca cgatgtcgcg gttatgtagc aagaatccgt cggcaatgcc
    18061 ctgcaaatcc gccagcgcct gttcgttgct gatagctggt ggtttaccgc tcaggttgcc
    18121 ggaggtcatc accagcgggc attgcagttc ctgtaacagc aaatgctgga gcgggttcgc
    18181 aggcaacatc accccgactt cgttaaggcc aggggcgata tcatcacaaa gctcaggtac
    18241 gtatttttta tccaccagca caatcggcgc agcgggcgtg gtaagcaact ggcgcgcagc
    18301 gtctggtaaa ctttctgcca ctggcaacat gactgccagc ggtttcgccg ggcgatgttt
    18361 gcgctcccgc agtgtcgcca ccgcgttact gttacgtgca tcgcaggcaa gatgaaatcc
    18421 gccaatccct ttgatggcga caatgttgcc catttttaac tgtgcgatag ctgcctgtaa
    18481 tgccgcctct tgttctgcat gttcaccatg acttacccat tcaagatgcg ggccacactc
    18541 cgggcaggcc accggctggg cgtggaagcg acgatcgagc gggtcgcggt actctttgtc
    18601 acaggccgga catagcggaa acgccgccat cacggtaaac gggcggtcgt aaggcatcgc
    18661 gcgaataatg gtgaaacgcg ggccgcagtg ggtacagttg ataaacggat aacgataacg
    18721 ccgctcgcct ggggtattca tttcggcaag gcaagcaggg caagtggcgg catccgggac
    18781 aatttgcgta ttcatggtgc cgccggtgct ctggcggatg gtgaactcgg tgggcagttg
    18841 tgaccagata aacggctcac gctcgacgct atcaatacgc gccagtggcg gacagtgctg
    18901 atgcaattga acaagaaacg tttccgggtc ttccagcagc cggacttcta cgccatcgcc
    18961 gtcattacat acatcgccgt gaagatttaa ttgctgtgcc agctgccaga caaacggacg
    19021 aaaaccgacg ccctgcactt tgccacgaat acgcagttgg acaccgcaag atgtgttttt
    19081 tgccattgag ttattcccgc catcatgaat tgcgtaaccc gccctgccgg acacgacagc
    19141 gtcgtatccg gcagtctcag gtcggcgatc ccgcccgctc cgtattctac gaatatttcc
    19201 gggaattcct ttgatgccag aacagttctg taagattttt agaacatcag cgccgtacgg
    19261 cggcgttttt ctgcgctcag ttgttcaagt ttattacgat cgacacaaat cagcgcatgg
    19321 gtcgggcaag ccgccataca cgccgggccg tcttcacgat ggttgcacag gtcgcattta
    19381 ttggcttcgg ctttgtcagc ccgtacattc agacccgcgc cgctgttgcg aatcacagga
    19441 cgtaccacca cttccatcgc gccatacggg caagccacca cgcaggtttt gcaaccaatg
    19501 caacgttcct gcatcacagg aacaaaccct ttatcacggc tgatagcacc attcgggcag
    19561 acgttagcgc acggtgcatc ttcacactga cggcaaactg tcgccgtgga aatgttcaca
    19621 cctttaatga catggatacg cggtaaaaat gtttccgggg tcagcgatgc acagtcctga
    19681 ttttcctgat gagaaaccac gcacgccact tcacaggtac ggcaaccaat acatttactc
    19741 gcgtcagcaa tgatgaaacg gttcatcaaa ttctccagca atgacagtta atgcgccgat
    19801 acattcacaa atcatgccag tttttaattt gctgttattt aaggaaatta atttctgtaa
    19861 tgcaggaaaa aagatgtcat cgacactagt gacgatgaca tgtgatgaca atgtttatcg
    19921 cgaaagggca atgagtgagt cgcggcggat cagttttccg ctaaaggttt tcggcggtga
    19981 gaaatccccg ccatcgagca taaaaatcag ccgtccaata atttcctgaa tcatctcagt
    20041 caccggaatt tttacgctgg agagcgccgg aacgatatag ggggcaattg cgatatcatc
    20101 gaatccgata actgacacct gctctggcac cgctacgccg cgctcgtgca acgctttgat
    20161 cgcacctatc gccatatcgt cgttactggc aactaacgcg ctaaatttag cccctcgttc
    20221 gagcaacgtt tctacccctt cggccccgct ggcgggcgtc catttaccgt tagcgataag
    20281 tttttcattg agcgcaatac catgctgcga cagcgcgtct ttatacccgg caagacgttc
    20341 aatgctggtg ggggaatcca tcgagccggt aaggaaagca atctcctgat gcccggcgtt
    20401 tatcaactct gccacggcgt taaagctggt ctgtttatga tcgcaccaga cgctatggct
    20461 gctattattg cgcaggcggc gattaagcac cattatcggc tgactgtgcg cgtcaatgat
    20521 gtcatcaatc tcatccacgc ttaaaaaacg cgggtaaatc ataatggcgt cgcagcgcaa
    20581 atccagcaga tactgaatcg cctggcgctc ttcttcggcg ctgtgtttac catctgccaa
    20641 tagcaactgt cgccctttct cttccgccat tcgcgcggca tgaaacagta attcactaaa
    20701 ataaatgccg tgataaagcg tgttggtcac taccagcccc agcgtctgag tactcttcgc
    20761 cgacagattg cgggccagca aatttgggcg gtaactgctc tcttctaccg cctgaaacac
    20821 gcgatcttta gtctcctggc tgacgtagcc attacctgaa agcacgcggg aaacggtcgc
    20881 ttttgaaacc ccggcgcgct tcgccacttc cagcatcgtc gtcatcattt tcatcccttt
    20941 acacgcaatc aacgcagtgt actgcaccgt ttgccgattg tccttgcgca atcggctcca
    21001 gcggataaga cgaggtcgcc gtgccggttt tgatgacttt tttgataaag gtaaacatgg
    21061 cgattcctta tttcagcggc gagtttttac gctcaatgct gtaacgctcg agttctttgt
    21121 acggcaccac tttgctcttc ttcttacgca catcgactac ggtcatgcgg tcggtacagg
    21181 agtagcaagg gtccaggcta ccgataatca gcggcgcatc ggaaacggta ttgccacgca
    21241 gcatgtagcg cagggtcggc cagttcgcgt aggtcgcggc acggcagcgc cagcggtaca
    21301 gcttctggtt gtcgccggtc atgctccagt ggatatcgtc gccgcgcggc gcttcggcaa
    21361 agcccagcgc gaaacggtgc ggaatgtagg taaagccttc caccatcagc ggaccgcccg
    21421 gcaggttatc cagaccgtag tcgatcatat tcagcgcggt atagacctcg ttgattcgca
    21481 ctttcagacg ggaaataacg tcgcagccct gctcgctgtg gacttccatt ggcagcaggc
    21541 cataaccgac aaacgggtga tcggcgcgag tatcgcgggc gagaccgctg gcgcgaacca
    21601 tcgggccaac gttactgaaa tcgcgggcga tttccgggtc cagacgacca atgccgacgg
    21661 tgcgttgttc catgttcggc gtactcagca gcacatccac cagctcctgc acttcacgac
    21721 gcatttgttg cgccagctgg cgggtctgga tcatgtcatc tttcagcaga tcgcgacgaa
    21781 tcccgccgat caggttcagg ccgtaggttt tacgcgcacc ggtaaggatc tctgccattt
    21841 tcatggaggt ttcacgcacg cggaagaact gcataaagcc ggagtcgaag ccggtaaagt
    21901 gacaggcgag gccgaggttg agcagatgcg ggtgcaggcg ttccacctcc agcagaatgg
    21961 cgcggatcat ctgcgcgcgt tctggcacct gaatacccat cgcgttttcc accgacgtgg
    22021 tgtaggcggt gctgtgggca aagccgcaga tcccgcacac acggtcagag aggaaggtca
    22081 cttcgttata acccatgcgg gtttccgcca gtttttccat accgcgatgg acgtagaaca
    22141 gacggtagtc ggcgtcgata atgttttcgc catcgacgaa cagacggaag tgacccggtt
    22201 cgtcagaagt gacgtgcagc ggaccaatcg gcacgacgtt gtttttcttg tcgcccagtt
    22261 cgttgatgaa ctcgtaggtt tcagcatcgg tagtcggtgc cggacgctga cgataatcca
    22321 tgctgtcttt acgcagcgga taaagttcat ccggccagtc atccggcagc accagacgac
    22381 gttcatccgg cagaccaacc ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc
    22441 acaccgccgc cggaacgcgc ggcgtcacgg acggatactc cggtttgttg gcgtcaactt
    22501 cgacgcgaac cgttacccaa cacttggtgc ccttctccat cgacagcacg taataaacgg
    22561 cgtaatgacc attcagtttg cgttcgtcgt taccaaacag caccgacagc cagccaccct
    22621 gcttgtagta gagaaactcc accacttccg gcagatagtt cacctttacg gtgacagtca
    22681 gctgatcttt ggtctgccag gcgtggtcca gcacgacgcc tagaaatgcc tcattcagcg
    22741 cggcgagata atgttgacct aatttttctt cagacatgct caaactctct ttaatcacgc
    22801 cgccagcaag gagacgaacg ctaaaaatgc aaagccaaac ccagcccagg taatgcgcgg
    22861 agtaatatca agacgcagac gggccatgct gttttcgaac agcgcgataa ccaggacgcc
    22921 gaccaccagt ttgacgatgg caatcaccag cgccagcagc agtccaccga cggtgaaggt
    22981 ttccatttgt ccccacggaa taaacacccc gacgaacatc tgcaacacca ccagctgttt
    23041 caggctgata ccccatttca tgacgccaaa gccgctaccg ctgtattcgg agagcggacc
    23101 ttcctgcaac tcctgctcgg cttccgccag gtcgaacggc agtttgccca tttcgataaa
    23161 agtggcgaac gcacaggcgc agagcgccag caccagcggg atgctttggc tcagcggcca
    23221 gtgataaacg gtgtcggtaa tgttgctgat gttggtggaa ccggcaacct gtgcggcaac
    23281 ccacagacca agcagcaaca tcggttcgac cagcacgcca agcatcgctt cacggctcgc
    23341 accgatagcg gtaaacgggc taccggtatc cagaccggaa atggcaaaga agaaacgcgc
    23401 gatggcgaag agatacagta aggtgatcaa atcgcccagt tgcggcagcg gagaaccgac
    23461 ggtcaccacc ggcagcgcag tagcgatagt cagcatgacg cccaccatca catacggcgt
    23521 caggcggaac atccagccag aggcatccgg gccaacgctc tggcggccca gcagtttgat
    23581 aatgtcgcga tactcctgca acacgcccgg cccgcgacgg ttatgcagac gggcgcgcgc
    23641 cacgcgggta ataccggaga gcagcggcgc aacggcaaat aacaccagcg cctgaattaa
    23701 cggatataaa acactcattc tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc
    23761 agttcaacca gcgccatccg gcggaacagc aacgcactac cttcgcactg ccagcccggc
    23821 actagcgaca ccggattcag ccatttgcgc agtttcagca ctggcgcaaa cgcctgttta
    23881 accggcatgg caaaaccgtg agcggtaatc accattgatt tttcatggtc gtagccgcac
    23941 acccaggccg caccgcggga acgtgacggc aggcggtcgc ctttgcaaat cgccataatg
    24001 atgaatggca gcagcgggca ggcaatcagc agcaatgtga tcattggttg agaaacggtg
    24061 gtgttagcag gctccagagg cagaggtaca gcagcagaga gcatcggcag cagccacggc
    24121 gcagcaacac cgccaattac gcagcaaatc gccagtgcca ctacgcttac gctcatcagg
    24181 agcggcgcac aggtggcgtt ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg
    24241 ccatagactt tcgccataca catcaccgcc agcgcaccgg taattgccag acccacagcg
    24301 agcagcggcc ccagcagacg ggcaacaaac gcgccgctat tgctcagttt gaagaaggat
    24361 tgatagataa cccattcccc ggcaaacccg ttcagtggcg gcagcgcagc cattgccatc
    24421 aacccgacta acatggcgat ggagataacc ggcattttct tgccaatacc accgagtttt
    24481 tcgatatcgc gatgaccggt acggaaccag acgctccccg ccccgaggaa cagtacgctt
    24541 ttgaacaggc tatggttaag cagatggtac agaccaccga ccaggccaag agcaatcagc
    24601 gccggttgtt cgagcgcgat acccgttacg ccagcgccca gccccagccc cagccccagc
    24661 cccagcagga taatgccgat attttccagg gtgtggtaag ccagcaggcg ctggatgttg
    24721 tgttccatca acgcatacag accaccgacg aacgcggtga tcatgccgag caccagcagc
    24781 gcgatccccc accacagcgg tgcattaccg cccagcagtg acagggttaa catgcccagc
    24841 aggccaattt tcatgactac cgtagaaaac aacgcggcag ctggcgcaga ggcgttagca
    24901 tgtgcctgcg gcacccagcc gtgcagcgga ataatcccag ccagcaggcc aaagccaatc
    24961 acaccgagca gccagatatc ggaaccgagc ggcagctgtt gcatacgcat atccagcagg
    25021 cgcagatcca gcgtgccata acgctgccac agcagccagc aagcaatcac cagcagcaga
    25081 gtgccaagac gccccagcgc aaaccacagt ttgccctctt tgctgttgct ggtgaggaac
    25141 accgcacaca aggccatgac ttcggccatt accacgaaca tgccgaggtt gctggcgatg
    25201 acggcgcaga cggcggcagc catcaacata ttgatctgca agccgttgca cttcacctgc
    25261 gcgtggcgat gccagtcaat gttatagagg ctgacaaaca gaccgcaaag gccgagggta
    25321 atcagccaaa tcgcgttaag cggggagatt tgcacatcgt agcttaccag cgacagcgca
    25381 ccgctcacgc caaccgcgcc agtcagtaca gtgaagcccg cggctgccgt atacagacta
    25441 ccaaccgcgc cgccaattcc ggctatccag ccacttagcg ctttttgaaa agagaaatgc
    25501 cagaacagcg gcggcgacaa accatgccac gccgctattg atcagggaaa ttgcgctcat
    25561 ttagcctctc cactttgagc ctgctgaaac aaggtgagat cgccaaagtc cgtgttaaag
    25621 gtcagctcac gcttacgttt gctgacgcgg gcgatatcgg tgttatccac cagatgcagg
    25681 gctttagtcg ggcacatccg cacacaggcc ggaccttgtt catcaaagct acaaaggtca
    25741 catttgacgg cgatcgcgcg aatacctggc acccagtcaa gcaatgtgct gacacgcgcc
    25801 ggagcaggcg gtgccggtgg cgctttcggg gtattggcgt ttgccggaat atccagcgga
    25861 cggctgccgg aaaattcaat tgcgccaaac gggcaggcga tgccgcacag tttgcagctt
    25921 acgcacagac tttcattcaa ctgcacggcc ccatcaacgc gggtgatggc gttaaccggg
    25981 cagaccaccg cgcagggtgc atcttcacag tggtgacaga gctgcggcgc agattctttt
    26041 tcattgagca tcactctcag gcgcggcatt gattgcaggc cgtgctggcg atgcgtctct
    26101 gaacaggcgg cctcacaggt gtggcagccg atacagagcg tggagtcggc aattacaaaa
    26161 cgattcacca ggcattcctc aggtgattgt catttttgac gaaaacatgc cgatgaaatg
    26221 tcattttcga cactcatcga cacgcccatc cccaaacagg cgtaacgcct gcaaaacggg
    26281 caaagcctca gctcatgctg ccgggctttg tccctttacc agttggctta aattcaccgg
    26341 cacattgttt tcaatggcgg tgtataagct gtcgtaaacg ccagcgattt tttcgagata
    26401 gtgttccaga acctgttcgc gatcgcggat gttgaccttg ccatcaatca tgccgtcaat
    26461 gctcagttgc gcctgcgcaa tccgctgttt gtcttcgcca tcttttgttt cgacgtaata
    26521 ctcgatggtg tggctgttaa agccatccgc cagggtgacg taaatacgaa aatgttcaaa
    26581 aaggacgaaa cagagttctt tgtccggcgc gcagctcatg gcgtgataca ggcgcgcttt
    26641 cgataacgtg atcccctgaa ccagcgaatt gcagtagatg tgccactggt cctgtaggcg
    26701 acgatgccgc tgtgcgatgt aatcggcttt ctcgcttatt tcccaaatag tcattgtcag
    26761 gttactcgtt taacagagat gccagcttta agcatttttt gtgccaactt ttaatttatt
    26821 gttattaaag agatttttaa gctaaagatg aatttcgtcg ccgtgtcgac gtgtcatttc
    26881 gacatcatcg acattattca ccgcagggat aatcaacact ggcgcaatta ttgcttgtag
    26941 ctggcaatag ttaatgggag gcgatatgca cgaaataacc ctctgccaac gggcactgga
    27001 attgatcgaa cagcaggccg caaaacacgg cgcaaaacgc gtaactgggg tctggctcaa
    27061 aattggcgca ttttcttgtg tcgaaaccag ctctcttgcc ttttgttttg atctggtttg
    27121 ccgcggcagc gtggcggaag gttgtaaact gcacctcgaa gaacaagagg ccgaatgctg
    27181 gtgtgaaaca tgccaacagt atgtgacgct actgacccag cgcgtccgcc gctgtccaca
    27241 gtgtcatggt gacatgctac agattgtggc agacgacggt ttacagattc ggcggataga
    27301 aatagaccag gagtgagcga tgtgtacaac atgcggttgc ggtgaaggca acctgtatat
    27361 cgagggtgat gaacataacc ctcattccgc gtttcgtagc gcgccatttg ccccggcggc
    27421 acgcccgcag atgaaaatca ccggcattaa agcgcctgaa tttaccccca gccagactga
    27481 agaaggcgac ctgcattacg gtcatggcga agcgggcact cacacaccgg gcatgagcca
    27541 gcgtcggatg ctggaagtcg aaattgacgt gctggacaaa aacaaccgtc tggctgaacg
    27601 caaccgcgcg cgctttgctg cccgcaagca actggtgctc aacctggttt ccagccctgg
    27661 ttccggtaaa accaccctgc tgacggaaac cttaatgcgc ctgaaagaca gcgttccgtg
    27721 cgcggttatt gaaggcgacc agcaaaccgt gaacgatgcc gcacgcattc gcgctaccgg
    27781 tacaccagcg attcaggtga acaccggtaa aggctgccat cttgacgcac agatgattgc
    27841 cgacgccgca ccgcgtctgc cactggacga taacggtatt ctgtttatcg aaaacgttgg
    27901 caacctcgta tgcccggcca gcttcgatct cggtgaaaaa cacaaagtag cggtgctttc
    27961 cgttaccgaa ggtgaagaca aaccgctgaa atatccgcat atgtttgccg ccgcctcgct
    28021 gatgctgctc aacaaagttg acctgttgcc gtatctcaac tttgacgttg agaagtgcat
    28081 cgcctgcgcc cgcgaagtca atccagaaat tgaaatcatc cttatttccg ccaccagcgg
    28141 cgaagggatg gaccagtggc tgaactggct ggagacacag cgatgtgcat aggcgttccc
    28201 ggccagatcc gcaccattga cggcaaccag gcgaaagtcg acgtctgcgg cattcagcgc
    28261 gatgtcgatt taacgttagt cggcagctgc gatgaaaacg gtcagccgcg cgtgggccag
    28321 tgggtactgg ttcacgttgg ctttgccatg agcgtaatta atgaagccga agcacgcgac
    28381 actctcgacg ccttacaaaa catgtttgac gttgagccgg atgtcggcgc gctgttgtat
    28441 ggcgaggaaa aataatgcgt tttgttgatg aatatcgcgc gccggaacag gtgatgcagt
    28501 taattgagca tctgcgcgaa cgtgcttcac atctctctta caccgccgaa cgccctctgc
    28561 ggattatgga agtgtgtggc ggtcataccc acgccatttt taaattcggc ctcgaccagt
    28621 tactgcctga aaacgttgag tttatccacg gtccgggttg cccggtgtgc gtactgccga
    28681 tgggtagaat cgacacctgc gtggagattg ccagccatcc ggaagtcatc ttctgtacct
    28741 ttggcgacgc catgcgcgta ccagggaaac agggatcgct gttgcaggca aaagcacgcg
    28801 gtgccgatgt gcgcatcgtt tactcgccga tggatgcgtt gaaactggcg caggagaatc
    28861 caacccgcaa agtggtgttc ttcggcttag gttttgaaac caccatgccg accaccgcca
    28921 tcactctgca acaggcgaaa gcgcgtgatg tgcagaattt ttacttcttc tgccagcaca
    28981 ttacgcttat cccgacgctg cgcagtttgc tggaacagcc ggataacggt atcgacgcgt
    29041 tcctcgcgcc gggccacgtt agtatggtta tcggcactga tgcctataat tttatcgcca
    29101 gcgattttca tcgtccgctg gtggtcgctg gtttcgaacc ccttgatcta ctgcaaggcg
    29161 tggtcatgct ggtggagcag aaaatagcgg cccacagcaa ggtagagaat cagtatcgtc
    29221 gggtggtacc ggatgccggt aacctgctgg cgcaacaggc gattgccgat gtgttctgtg
    29281 tcaacggcga cagcgaatgg cgcggcttag gcgtgattga atcttctggc gtgcacctga
    29341 cgccggatta tcaacgattc gatgccgaag cacatttccg cccggcaccg cagcaggtct
    29401 gcgatgaccc gcgcgcgcgt tgtggtgagg tattaacggg caaatgtaag ccgcatcaat
    29461 gcccgctgtt tggtaacacc tgtaatcctc aaaccgcatt tggtgcactg atggtctcct
    29521 ccgaaggagc gtgcgccgcg tggtatcagt atcgtcagca ggagagtgaa gcgtgaataa
    29581 tatccaactc gcccacggca gcggcggcca ggcgatgcag caattaatca acagcctgtt
    29641 tatggaagcc tttgccaacc cgtggctggc agagcaggaa gatcaggcac gtcttgatct
    29701 ggcgcagctg gtagcggaag gcgaccgtct ggcgttctcc accgacagtt acgtcattga
    29761 cccgctgttc tttcctggcg gtaatatcgg caagctggcg atttgcggca ccgcgaatga
    29821 cgttgcgggc agtggcgcta ttccgcgcta tctctcctgt ggctttatcc tcgaagaagg
    29881 attgccgatg gagacactga aagccgtagt gaccagcatg gcagaaaccg cccgcacggc
    29941 aggcattgcc atcgttactg gcgatactaa agtggtgcag cgcggcgcgg cagataaact
    30001 gtttatcaac accgctggca tgggcgcaat tccggcgaat attcactggg gcgcacagac
    30061 gctaaccgca ggcgatgtat tgctggttag cggtacactc ggcgaccacg gggcgactat
    30121 ccttaacctg cgtgagcagc tggggctgga tggcgaactg gtcagcgact gcgcggtgct
    30181 gacgccgctt attcagacgc tgcgtgacat tcccggcgtg aaagcgctgc gtgatgccac
    30241 ccgtggtggt gtaaacgcgg tggttcatga gttcgcggca gcctgcggtt gtggtattga
    30301 aatttctgaa tcagcgctgc cggttaaacc tgccgtgcgc ggcgtttgcg aattgctggg
    30361 actggacgcc ctgaactttg ccaacgaagg caaactggtg atcgccgttg aacgcaacgc
    30421 ggcagagcaa gtgctggcag cgttacattc ccatccactg gggaaagacg cggcgctgat
    30481 tggtgaagtg gtggaacgta aaggtgttcg tcttgccggt ctgtatggcg tgaaacgaac
    30541 cctcgattta ccacacgccg aaccgcttcc gcgtatatgc taataaaatt ctaaatctcc
    30601 tatagttagt caatgacctt ttgcaccgct ttgcggtgct ttcctggaag aacaaaatgt
    30661 catatacacc gatgagtgat ctcggacagc aagggttgtt cgacatcact cggacactat
    30721 tgcagcagcc cgatctggcc tcgctgtgtg aggctctttc gcaactggta aagcgttctg
    30781 cgctcgccga caacgcggct attgtgttgt ggcaagcgca gactcaacgt gcgtcttatt
    30841 acgcgtcgcg tgaaaaagac acccccatta aatatgaaga cgaaactgtt ctggcacacg
    30901 gtccggtacg cagcattttg tcgcgccctg atacgctgca ttgcagttac gaagaatttt
    30961 gtgaaacctg gccgcagctg gccgcaggtg ggctataccc caaatttggt cactattgcc
    31021 tgatgccact ggcggcggga gggcatattt ttggtggctg tgaatttatt cgttatgacg
    31081 agcgtccctg gagcgaaaaa gagttcaatc gtctgcaaac atttacgcag atcgtttctg
    31141 tcgtcaccga acaaatccag agccgcgtcg tcaacaatgt cgactatgag ttgttatgcc
    31201 gggagcgcga taacttccgc atcctggttg ccattaccaa cgcggtgctt tcccgcctgg
    31261 atatggacga actggtcagc gaagtcgcca aagaaatcca ttactatttc gacattgacg
    31321 atatcagcat tgtcttacgc agccaccgta aaaacaaact caacatctac tccactcact
    31381 atcttgataa acaacatccc gcccacgaac agagcgaagt cgatgaagcc ggaaccctca
    31441 ccgaacgcgt gttcaaaagt aaagagatgc tgctgatcaa tctctacgag cgggacgatt
    31501 tagcccccta tgaacgcatg ttgttcgaca cctggggcaa ccagattcaa accttgtgcc
    31561 tgttaccgct gatgtctggc gacaccatgc tgggcgtgct gaaactggcg caatgcgaag
    31621 agaaagtgtt taccactacc aatctgaatt tgctgcgcca gattgccgaa cgtgtggcaa
    31681 tcgctgtcga taacgccctc gcctatcagg aaatccatcg tctgaaagaa cggctggttg
    31741 atgaaaacct cgccctgacc gagcagctca acaatgttga tagtgaattt ggcgagatta
    31801 ttggccgcag cgaagccatg tacagcgtgc ttaaacaagt tgaaatggtg gcgcaaagtg
    31861 acagtaccgt gctgatcctc ggtgaaactg gctcgggtaa agagctgatt gcccgtgcga
    31921 tccataatct cagtgggcgt aataatcgcc gcatggtcaa aatgaactgc gcggcgatgc
    31981 ctgccggatt gctggaaagc gatctgtttg gtcatgagcg tggggctttt accggtgcca
    32041 gcgcccagcg cattggtcgt tttgaactgg cggataaaag ctctctgttc ctcgacgaag
    32101 tgggcgatat gccactggag ttacagccga agttgctgcg tgtattgcag gaacaggagt
    32161 ttgaacgtct cggcagcaac aaaatcattc agacggacgt acgtttaatt gccgcgacta
    32221 accgcgatct gaaaaaaatg gtcgccgacc gagagttccg tagcgatctc tattaccgcc
    32281 tgaacgtatt cccgattcac ctgccgccac tacgcgagcg tccggaagat attccgctgc
    32341 tggcgaaagc ctttaccttc aaaattgccc gtcgtctggg gcgcaatatc gacagcattc
    32401 ctgccgagac gctgcgcacc ttgagcaaca tggagtggcc gggtaacgta cgcgaactgg
    32461 aaaacgtcat tgagcgcgca gtattgctaa cacgcggcaa cgtgctgcag ctgtcattgc
    32521 cagatattgc tttaccggag cctgaaacgc cgcctgccgc aacggttgtc gcccaggagg
    32581 gcgaagatga atatcagttg attgtgcgcg tgctgaaaga aaccaacagc gtggttgccg
    32641 ggcctaaagg cgctgcgcaa cgtctggggc tgaaacgcac gaccctgctg tcacggatga
    32701 agcggctggg aattgataaa tcggcattga tttaactgca aattgccgga cagatctgcc
    32761 tgtccggcat actattcacg aggttttttc ggacgatatt tttccggcag ttctggcacc
    32821 ggacgcttgt catcgatgag atgacgcacg gttaagatcg gatgacgcca cagcattctc
    32881 ggcccggccc aacgcataat ctgtttcatc tcttcacgct ttgcaggctg gtaacagtgc
    32941 accggacact gcttacaggc tggtttctct tcgccgaaca cacatttatc cagccgcttt
    33001 tgcgcgtaaa caaacaacgc ctcgtaatgc tccggctccg ctgacgcctg cgggcatttc
    33061 gcttgataaa gatcgatcat ttttttaatc gtcagttttt cacgagagat acgcttgccg
    33121 gacatgctgc ctccacctca ttaagatgca tttatattac atcttaatc
//
LOCUS       DAJVRP010000038        32634 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVRP010000038 DAJVRP010000000
VERSION     DAJVRP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.038"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..956)
                     /locus_tag="OQN12_001836"
     CDS             complement(<1..956)
                     /locus_tag="OQN12_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCS1654189.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="OQN12_001837"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="OQN12_001837"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCS1654190.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OQN12_001838"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OQN12_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OQN12_001839"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OQN12_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1654191.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="OQN12_001840"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="OQN12_001840"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1654192.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="OQN12_001841"
     CDS             complement(5018..6346)
                     /locus_tag="OQN12_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1654193.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="OQN12_001842"
     CDS             complement(6354..8702)
                     /locus_tag="OQN12_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1654194.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="OQN12_001843"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="OQN12_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1654195.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="OQN12_001844"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="OQN12_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1654196.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="OQN12_001845"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="OQN12_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1654197.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="OQN12_001846"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="OQN12_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1654198.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="OQN12_001847"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="OQN12_001847"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1654199.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="OQN12_001848"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="OQN12_001848"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1654200.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="OQN12_001849"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="OQN12_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1654201.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="OQN12_001850"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="OQN12_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1654202.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19172
                     /locus_tag="OQN12_001851"
     CDS             18273..19172
                     /locus_tag="OQN12_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1654203.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19178..21610
                     /locus_tag="OQN12_001852"
     CDS             19178..21610
                     /locus_tag="OQN12_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1654204.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21756..22571
                     /gene="ybiV"
                     /locus_tag="OQN12_001853"
     CDS             21756..22571
                     /gene="ybiV"
                     /locus_tag="OQN12_001853"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1654205.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22723..23988
                     /locus_tag="OQN12_001854"
     CDS             22723..23988
                     /locus_tag="OQN12_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1654206.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24229..25821)
                     /locus_tag="OQN12_001855"
                     /pseudo
     CDS             complement(24229..25821)
                     /locus_tag="OQN12_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
     gene            26040..26960
                     /gene="ldtB"
                     /locus_tag="OQN12_001856"
     CDS             26040..26960
                     /gene="ldtB"
                     /locus_tag="OQN12_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1654207.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27018..28136)
                     /locus_tag="OQN12_001857"
     CDS             complement(27018..28136)
                     /locus_tag="OQN12_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1654208.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="OQN12_001858"
     CDS             complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="OQN12_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1654209.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28786..28914
                     /gene="mntS"
                     /locus_tag="OQN12_001859"
     CDS             28786..28914
                     /gene="mntS"
                     /locus_tag="OQN12_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1654210.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29186..30769
                     /gene="opgE"
                     /locus_tag="OQN12_001860"
     CDS             29186..30769
                     /gene="opgE"
                     /locus_tag="OQN12_001860"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1654211.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="OQN12_001861"
     CDS             complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="OQN12_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1654212.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31385..31450)
                     /locus_tag="OQN12_001862"
     CDS             complement(31385..31450)
                     /locus_tag="OQN12_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654213.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31685..32572
                     /gene="rhtA"
                     /locus_tag="OQN12_001863"
     CDS             31685..32572
                     /gene="rhtA"
                     /locus_tag="OQN12_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1654214.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8168 a         8700 c         7883 g         7883 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 ccccggaaaa accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc
    19141 agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac
    19201 gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt
    19261 ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat
    19321 cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa
    19381 acacgatgag ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc
    19441 ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc
    19501 tggctttgcg gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc catggtggcg
    19561 cggtcagacg gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct
    19621 ggcgaccggc atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt
    19681 caatttcccg ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg
    19741 tcgctcgcgc atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc
    19801 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    19861 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    19921 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    19981 ttacttcatc cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg
    20041 tatggatcag tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga
    20101 tcgcgaacac gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa
    20161 aatccgctcc ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac
    20221 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    20281 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    20341 ccatgcagga atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt
    20401 cgggatgccg gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat
    20461 tgaaccgcag gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg
    20521 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    20581 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    20641 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    20701 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    20761 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    20821 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa
    20881 cctcggcaac agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca
    20941 gcaacagctg gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg
    21001 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct
    21061 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta
    21121 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    21181 accgtttggc gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc
    21241 agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc
    21301 agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa
    21361 tccggcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt
    21421 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    21481 ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc
    21541 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca
    21601 tatgctgtaa tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca
    21661 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    21721 tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag
    21781 atatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc
    21841 aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt
    21901 accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    21961 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    22021 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    22081 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    22141 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    22201 tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc
    22261 tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    22321 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    22381 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    22441 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    22501 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt
    22561 ttaacagctg acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct
    22621 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg
    22681 caaattagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    22741 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    22801 gcgcagcttg gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    22861 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    22921 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    22981 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    23041 ctggactatc tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    23101 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    23161 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    23221 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    23281 cgtatccgcc gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    23341 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat
    23401 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    23461 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc
    23521 tcggatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    23581 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    23641 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    23701 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    23761 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    23821 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    23881 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    23941 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    24001 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    24061 ctttcttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc
    24121 aatatattac aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt
    24181 gtcatcagcc gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc
    24241 tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat
    24301 ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag
    24361 cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg
    24421 gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag
    24481 catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct
    24541 gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca
    24601 ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctaggc
    24661 gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc
    24721 gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag
    24781 tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc
    24841 tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg
    24901 gataaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg
    24961 gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa
    25021 agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg
    25081 cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc
    25141 gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat
    25201 gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat
    25261 gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg
    25321 cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg
    25381 ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc
    25441 gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat
    25501 ttccggcaaa gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc
    25561 catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt
    25621 accaatgcgc tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc
    25681 gagaatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt
    25741 gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt
    25801 gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta
    25861 tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc
    25921 aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg
    25981 cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca
    26041 tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta
    26101 ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc
    26161 aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc
    26221 agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag
    26281 gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca
    26341 tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta
    26401 tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca
    26461 aagttgagca taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt
    26521 accgcgctgc gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg
    26581 gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg
    26641 gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc
    26701 tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga
    26761 ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag
    26821 cccagtttga aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga
    26881 ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga
    26941 tgccggttcg cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct
    27001 tttttatttg ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc
    27061 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
    27121 ttcgccatac gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc
    27181 acgtttaccg cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta
    27241 ctcggcacgt tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca
    27301 gataaatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
    27361 acgtcgataa acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg
    27421 cgacgcgcca gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg
    27481 aactccagcg ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt
    27541 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
    27601 cagcacaaca gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata
    27661 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
    27721 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
    27781 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
    27841 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
    27901 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
    27961 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
    28021 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
    28081 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
    28141 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
    28201 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
    28261 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
    28321 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
    28381 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
    28441 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
    28501 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
    28561 tcactttttt tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
    28621 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
    28681 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
    28741 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
    28801 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
    28861 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt
    28921 cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa
    28981 accacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
    29041 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
    29101 acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
    29161 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
    29221 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
    29281 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
    29341 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
    29401 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
    29461 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
    29521 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
    29581 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
    29641 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
    29701 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
    29761 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
    29821 taagccgaag tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct
    29881 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
    29941 atttgctgac tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
    30001 tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
    30061 gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat
    30121 cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc
    30181 agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
    30241 gcactcgcaa ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga
    30301 caggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
    30361 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
    30421 cagcagcttc tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa
    30481 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
    30541 ggtcatttcc agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga
    30601 cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata
    30661 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
    30721 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
    30781 cgccccgaga ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc
    30841 aatccaagtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    30901 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    30961 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac
    31021 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    31081 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    31141 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    31201 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    31261 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    31321 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    31381 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    31441 acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag
    31501 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    31561 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    31621 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    31681 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    31741 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag
    31801 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    31861 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    31921 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    31981 tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc
    32041 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    32101 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    32161 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    32221 agcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    32281 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    32341 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    32401 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    32461 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    32521 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
    32581 ttctgtatgg ttatgcataa ccatacagaa tttctcgcta cttttcctct acac
//
LOCUS       DAJVRP010000039        32620 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVRP010000039 DAJVRP010000000
VERSION     DAJVRP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.039"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            688..1116
                     /gene="uspC"
                     /locus_tag="OQN12_001864"
     CDS             688..1116
                     /gene="uspC"
                     /locus_tag="OQN12_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCS1654215.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1123..2537)
                     /gene="otsA"
                     /locus_tag="OQN12_001865"
                     /pseudo
     CDS             complement(1123..2537)
                     /gene="otsA"
                     /locus_tag="OQN12_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2512..3312)
                     /gene="otsB"
                     /locus_tag="OQN12_001866"
     CDS             complement(2512..3312)
                     /gene="otsB"
                     /locus_tag="OQN12_001866"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCS1654216.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3479..4465)
                     /gene="araH"
                     /locus_tag="OQN12_001867"
     CDS             complement(3479..4465)
                     /gene="araH"
                     /locus_tag="OQN12_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1654217.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4480..5994)
                     /gene="araG"
                     /locus_tag="OQN12_001868"
     CDS             complement(4480..5994)
                     /gene="araG"
                     /locus_tag="OQN12_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS1654218.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6064..7053)
                     /gene="araF"
                     /locus_tag="OQN12_001869"
                     /pseudo
     CDS             complement(6064..7053)
                     /gene="araF"
                     /locus_tag="OQN12_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7850..8353
                     /locus_tag="OQN12_001870"
     CDS             7850..8353
                     /locus_tag="OQN12_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1654219.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8433..8684)
                     /locus_tag="OQN12_001871"
     CDS             complement(8433..8684)
                     /locus_tag="OQN12_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1654220.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8799..8885)
                     /gene="azuC"
                     /locus_tag="OQN12_001872"
     CDS             complement(8799..8885)
                     /gene="azuC"
                     /locus_tag="OQN12_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1654221.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9148..9471
                     /locus_tag="OQN12_001873"
     CDS             9148..9471
                     /locus_tag="OQN12_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1654222.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9642..10139
                     /gene="ftnA"
                     /locus_tag="OQN12_001874"
     CDS             9642..10139
                     /gene="ftnA"
                     /locus_tag="OQN12_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1654223.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10176..10415)
                     /locus_tag="OQN12_001875"
     CDS             complement(10176..10415)
                     /locus_tag="OQN12_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1654224.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10605..11816
                     /gene="tyrP"
                     /locus_tag="OQN12_001876"
     CDS             10605..11816
                     /gene="tyrP"
                     /locus_tag="OQN12_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1654225.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11878..12543)
                     /gene="yecA"
                     /locus_tag="OQN12_001877"
     CDS             complement(11878..12543)
                     /gene="yecA"
                     /locus_tag="OQN12_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1654226.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12739..12825)
                     /locus_tag="OQN12_001878"
     tRNA            complement(12739..12825)
                     /locus_tag="OQN12_001878"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12789..12791),aa:Leu,seq:taa)
     gene            complement(12838..12911)
                     /locus_tag="OQN12_001879"
     tRNA            complement(12838..12911)
                     /locus_tag="OQN12_001879"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12877..12879),aa:Cys,seq:gca)
     gene            complement(12966..13041)
                     /locus_tag="OQN12_001880"
     tRNA            complement(12966..13041)
                     /locus_tag="OQN12_001880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13006..13008),aa:Gly,seq:gcc)
     gene            complement(13193..13741)
                     /gene="pgsA"
                     /locus_tag="OQN12_001881"
     CDS             complement(13193..13741)
                     /gene="pgsA"
                     /locus_tag="OQN12_001881"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1654227.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13798..15630)
                     /gene="uvrC"
                     /locus_tag="OQN12_001882"
     CDS             complement(13798..15630)
                     /gene="uvrC"
                     /locus_tag="OQN12_001882"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1654228.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15627..16283)
                     /gene="uvrY"
                     /locus_tag="OQN12_001883"
     CDS             complement(15627..16283)
                     /gene="uvrY"
                     /locus_tag="OQN12_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1654229.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16579..16755
                     /locus_tag="OQN12_001884"
     CDS             16579..16755
                     /locus_tag="OQN12_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654230.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16742..16966
                     /locus_tag="OQN12_001885"
     CDS             16742..16966
                     /locus_tag="OQN12_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1654231.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(16999..17619)
                     /gene="sdiA"
                     /locus_tag="OQN12_001886"
                     /pseudo
     CDS             complement(16999..17619)
                     /gene="sdiA"
                     /locus_tag="OQN12_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17849..18601)
                     /gene="yecC"
                     /locus_tag="OQN12_001887"
     CDS             complement(17849..18601)
                     /gene="yecC"
                     /locus_tag="OQN12_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1654232.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18598..19266)
                     /gene="tcyL"
                     /locus_tag="OQN12_001888"
     CDS             complement(18598..19266)
                     /gene="tcyL"
                     /locus_tag="OQN12_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1654233.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19281..20267)
                     /gene="dcyD"
                     /locus_tag="OQN12_001889"
     CDS             complement(19281..20267)
                     /gene="dcyD"
                     /locus_tag="OQN12_001889"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1654234.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20372..21172)
                     /gene="tcyJ"
                     /locus_tag="OQN12_001890"
     CDS             complement(20372..21172)
                     /gene="tcyJ"
                     /locus_tag="OQN12_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1654235.1"
                     /translation="MKLAYLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21260..21811)
                     /gene="fliZ"
                     /locus_tag="OQN12_001891"
     CDS             complement(21260..21811)
                     /gene="fliZ"
                     /locus_tag="OQN12_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS1654236.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21857..22576)
                     /gene="fliA"
                     /locus_tag="OQN12_001892"
     CDS             complement(21857..22576)
                     /gene="fliA"
                     /locus_tag="OQN12_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS1654237.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22741..24393)
                     /gene="fliC"
                     /locus_tag="OQN12_001893"
     CDS             complement(22741..24393)
                     /gene="fliC"
                     /locus_tag="OQN12_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS1654238.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24659..26065
                     /gene="fliD"
                     /locus_tag="OQN12_001894"
     CDS             24659..26065
                     /gene="fliD"
                     /locus_tag="OQN12_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1654239.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26090..26500
                     /gene="fliS"
                     /locus_tag="OQN12_001895"
     CDS             26090..26500
                     /gene="fliS"
                     /locus_tag="OQN12_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1654240.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26500..26865
                     /gene="fliT"
                     /locus_tag="OQN12_001896"
     CDS             26500..26865
                     /gene="fliT"
                     /locus_tag="OQN12_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCS1654241.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26943..28430
                     /gene="amyA"
                     /locus_tag="OQN12_001897"
     CDS             26943..28430
                     /gene="amyA"
                     /locus_tag="OQN12_001897"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1654242.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28464..28877)
                     /locus_tag="OQN12_001898"
     CDS             complement(28464..28877)
                     /locus_tag="OQN12_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1654243.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29064..30269
                     /gene="yedE"
                     /locus_tag="OQN12_001899"
     CDS             29064..30269
                     /gene="yedE"
                     /locus_tag="OQN12_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1654244.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30266..30499
                     /gene="yedF"
                     /locus_tag="OQN12_001900"
     CDS             30266..30499
                     /gene="yedF"
                     /locus_tag="OQN12_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1654245.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30606..31276
                     /locus_tag="OQN12_001901"
                     /pseudo
     CDS             30606..31276
                     /locus_tag="OQN12_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31316..32524)
                     /locus_tag="OQN12_001902"
                     /pseudo
     CDS             complement(31316..32524)
                     /locus_tag="OQN12_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         8170 a         8333 c         7714 g         8403 t
ORIGIN      
        1 aatagttgcg ataagctgca ataagcagag ccaccttttt ggtttaataa gtccttacaa
       61 atagaagggg gtctttacac ttatctaaga tttttcctaa atcgacgcaa ctgtactcgt
      121 cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg atgcagatca
      181 cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt tgggtgtatt
      241 tcaatctgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag gaataatcga
      301 aatgggatgt tgcgcacggt taaaataact caccgtaaat aatcatctgc tataaataat
      361 cactttcatg caataccaga taagctattt ttaaacagac acttaccgca caacaaacta
      421 attaaataca aagaaaggca acaatcaact taatgttaat aaaatgtttt tcatatatta
      481 accacttgtt tgatgcgggg aatcagtaca ttattttctt ataattacag ttgaaatcat
      541 atgatcaccg agagtgattg attaattgtc tttactaata atcagactaa tatttacctg
      601 tttgactgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa tgtcacctga
      661 agttcacagc agaataagga acaggttatg agctatagca atattcttgt cgctgttgcg
      721 gttacaccgg aaagtcagca actgctggca aaagcggtat ctatcgccag gccagtaaag
      781 ggacacatca gtttaattac tctcgcttcc aacccggaaa tgtacaatca attagccgcg
      841 ccgatgcttg aagatttacg cagcgtgatg caagaagaaa cgcaaagctt tcttgataag
      901 ttaattcagg atgcggggta tcccgttgac aagacgttta ttgcctacgg tgagttaagc
      961 gaacatattc tggaagtatg tcacaaacat catttcaatt tggtgatttg cggtaatcac
     1021 aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa gagtgattgc ctcaagtgag
     1081 gtcgatgtgc tgttagttcc acttacggga gattaaccgc tcctacgcaa gctttggaaa
     1141 ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc ggaactatct gctttaggtc
     1201 gctaatgaag cactcctgcc agtggttaat atcgtttttc acgataacgt ccagcatttc
     1261 tgcatgacgg gaaatacgtt ccaccagcga catagtcaat gcacgatcca gcgcagctgc
     1321 aacttcgtca cgatcgtagg ggttaacaat taacgccgac gttaactcgt ttgccgctcc
     1381 cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg tcctgagcag caacatactc
     1441 ttttgctacc aggttcatcc cgtcacgcag tggcgtcact aagcccacgt cagagtagcg
     1501 gaatattttc atcagtaatt tacggtcaaa atgctgattc aaataataaa gcggcgtcca
     1561 gcctaattgc ccgtatttac cattaattcg tccagcttca ttttcgagct gatgacgaat
     1621 atcctggtag gcttgcacat caccacgcga cgttggtgca atctgggtat aacgaatttt
     1681 accatgatgc tgcggatatt tttccagcaa tgcctcatag gcgagaaaac gctccggcaa
     1741 acctttggaa taatccagcc gttcgacaga aaagatattt tgtacgtttt tcaattccgc
     1801 tttaagttgc gccagttttg gcggcagtgg cccggcagcc tgtttggcta tttctttcgg
     1861 ttcaatgccg atcgggtaga cttctgttcg aaatgctttt ccccaggctg tatggctttt
     1921 tgcgctacgt gtcgtgacgc gggtcaggtt agaaaggcaa tccaggaacg ccagacgatc
     1981 gttttctgtc tgtaaaccca gcaaatcata atcacaaagc tgttcaagca aggtgtcata
     2041 tgtcggcagc gcgttgaaaa tttccggtgt cgggaaagga atatgcaaga aaaaaccaat
     2101 gcgattattc accccacgtt tgcgtaattc atgcgcaaag ggcaacaggt gataatcgtg
     2161 gatccagata atgtcatcgt cttgcaacag cggcagtaat ttatctgcca gcaacgcatt
     2221 tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc accagatcga gccgataatg
     2281 aaaagcgggc cagagaacgg cattggagaa ttggttgtag tattcgtcaa ggtcctgttc
     2341 gctgaggtta aaagaggccc acgtaatgtt acctttttta gcggctgatc ctcattccct
     2401 gtttcaccac tccagccaaa ccacagtccg cctgcggctt tcagtgcccc cagtatgcca
     2461 acggcaaggc caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg gttagatact
     2521 acgactaaac gactcatagt catcactcct gttattttct cttttttgtt gtaatgcggc
     2581 ggttatcatt tcaagccagc tccagacatc cggcacaccc gccagtcgcc atgatgcctg
     2641 agttgcacct gtgccaattt ttactgacat tccgcccagt cggttaacga ctgcgaagcc
     2701 agattcatcg gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa aaggagcttc
     2761 ctgcataaag gctgcaatcg cttcaccttt actggtacct ctcggtttga tctcgacaac
     2821 acactttccc tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt gcgctaatgt
     2881 cattaatgcg tcttcatgct gcggagcctg acgataatgc agcgcaaaag ccatcccttt
     2941 cgcctccagc tccgcgccgg gatactgagc gatgaccgta tgcagttgca cgctaatatc
     3001 acgcgcaatc gcatccggca gatgaacgat atgtgtttta ccattgatgt cacggcgctc
     3061 cgccccatgc acgcccgcta acgggaagcg ataaggtttt gccagtgcgt caagctccac
     3121 cattgagcgc cctgatatca atgccaatgc accatcactt gcggttgcca gtagctgtag
     3181 tccttgcaga atattgtcag gcacgacgac ctgatcggga tgcggtttga tttccgccag
     3241 cgttccatca agatcaaaaa accaggcata tttcgcggat agttcagggg tttcggttaa
     3301 cggttctgtc acccggctct cctccttctc ttttcttatt gtgttgcgca gaccgcgctt
     3361 tacagacatg taagtataga cagtgtgacg gaggtcgcca tttgcaacga agaacagcca
     3421 accgttgtgg cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga aaaaagcatc
     3481 agacagtgcg cttcgctttt tgcttgtaac ggtcgaagat caccgctgcc agcaggatta
     3541 agccgcgaac cacgtactgc gcgaaaggag aaatattaag caggttcatg gcgttttcca
     3601 cggtgcctaa aattaagata cccgccacca catatgagat ttttccgatg ccacctttca
     3661 gagaaacgcc acctaaaacg caggcggaga taacaataag ctcataacca atcgacgtca
     3721 ttggctgccc actagtcata cgtgaagcca gaataattcc ggctatcgca gataccaggc
     3781 cagagagaac aaagataata attttggtgc gaacaaccgg tatacccgcc agacgcgcag
     3841 cctcttcgtt cccgccaatc gccagggtat tacgaccaaa ggtggtttta ttcagcagca
     3901 aaccaaagat aatcagacac gcgacggtga gccagattgg cgcaggcaga ccgaaccagt
     3961 tagcgtaacc aagggcaaag aagctttcat cttcgatacc gaccgcttta ccgtctgaaa
     4021 tgatgtacgc cagacctcga acaatctgca tcgttgccag tgtcgtgatc agagcattta
     4081 ttttcagttt ggcgataaca aagccattga ccaggccaca gagaacgccc agcaacaaac
     4141 ccgctgccac gccaatccac aggctttcag tcaggttgat aaccaccgcc gtggtgacac
     4201 ccgcacaggc aattacggag gcgacagaaa ggtcaaagtc accggaagca aggcagaaca
     4261 acatgccaca cgccaccatc cccgacatgg aaattgccag gcccaaccct ttcatattaa
     4321 tgaaggtggc aaaatttggg acaaaaatgg cacaggcgat aaagagcacc gcaaacacca
     4381 ccagcatgcc gtactgatcc cagatacgcc cgaagctgaa tgacgactta ggtgcgccag
     4441 accccgatgt agaaacagaa gacatcatac tctccttact caggcaactg cctggctgac
     4501 tttaggcatc gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta acaattcacc
     4561 ggcgatttcg ccttcccgca tcaccacaat ccggtcggca acgccgagga cttcaggtaa
     4621 gtcgctggag gcaaacagca ccgccacgcc ctgcgccgcc agcgcataaa ttacgttata
     4681 tatttcgtgc ttagcgccaa catcaatgcc gcgcgtaggt tcatccagca aaatgacctt
     4741 catctcttcc gataaccagc ggcccagaat ggctttttgc tgatttccgc ctgagagatt
     4801 catgatcagt tgctccgcgc ccggcgtttt gatgttgagc gaacgaatgt gctgatcggc
     4861 attgttttct tcccaactgt tgttgattac acaaccgccg agcacatgtt tgcgtctggc
     4921 actgatgttg atattgtcgc gaacggagtg cacgggaata atgccttccg ctttgcgagc
     4981 ttccgggcag agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa tatcgatcgg
     5041 ctgttggtcg atataaacct gaccggcggt gatttgcgtc ccgccaaaca agcctttcat
     5101 taattcgcta cgccccgccc ctaccagacc aaacagccca acaatttcac cactgcgaac
     5161 cgccagactt attggcgtac gcacgcctag tgctttcaca gcatcaagac gcaggcgctc
     5221 ctcgccataa ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc cgaccatcgc
     5281 ctgcaccagc gcgtcgtggt caacctgctg catatcggta aaggttttga cataacgtcc
     5341 atctttaaag acagtgatgg catcgctgag ggcaaatatt tcttccatac ggtgagaaac
     5401 gtataagatc acccgcccct cttttcgcag ttcacgaata acgcggaaaa gattgccgat
     5461 ttcgcgggca gaaagggagc tggttggctc atcaaaggcg ataattttag cgttacgcgc
     5521 cagcgctttg gcgatttcaa ccatctgcca ctgaccaatg gcgagatatt tcagcggcgt
     5581 gtccgggtca atatccatac caagatgttt aagttgtaaa cccgcctcat aattcagcaa
     5641 tgagcgattc acaatgccgc ctttatgcgg cagctggccg agatagatgt tttccgcgac
     5701 ggtcatttcc ggcacgagat gcagttcctg gtaaataatc gccacacccg cgttaagtgc
     5761 tgcggtcgtg tcggaaaagg acatttcctg cccattaatc actacagaac ccgtggttgg
     5821 cgcatagttg ccgctgagga tttttaagag agttgatttt cctgcgccat tttcacccat
     5881 caacgcatga acctgaccgg catagcagtc aaaactaata tccgtcagcg ccttaacgcc
     5941 gggaaacgtt ttaccgatgc cgcgaaatga gagatacggg gtagactgtt gcataacgtc
     6001 tccgtgaatc actggtcgtt cacaccccct ctgcatcatg cagaggggaa tttttccggc
     6061 aaattactta ccgcctaaac cttttttctc cagttcttct ttaaagttgt cacgcgtgat
     6121 cagtaccacg tctgtaactt cggtaaattt tggcgtttca acgtcttttg ctacccagtt
     6181 gtaaagcatt tcgctggatt tatagccatg tacgtccggg cttggcagca gggaaccgta
     6241 gaagccggtt gcctgtgctt tagacagttc gcttactgca tccacaccgt taataccaat
     6301 accgatgata tcggctgctt taaagccctg accttcggtt gcacgtacgc cgccaagtac
     6361 ggtgctgtcg ttcataccga cgatcagcca atgtttaact tccggatgtt gaaccagcat
     6421 tgagttggca gcgtcaaatg cccccgggat gtcgttagat ttggtaggta cctgataaat
     6481 ttgtttttcc gggaatcctg cagctttcag tgcgtccata gaacccgttg tacggcggcg
     6541 ggcggtatcc agttcgttgg cggtaatcgc catcactgcg ctttctttga catcccagcc
     6601 acgtttctgc atctctttat acagttccta gccctgacgt tcgccaattt tagtcgccgc
     6661 catcatcacc agaggaacgg tatccattgg cttacctttg gcgttaacaa actggtcatc
     6721 cactgcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag agccgagttt
     6781 cgggtccgga gtacaaataa cgaaaccttt tgcgccactg gctgccaggc tgtcgatcgc
     6841 gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc ctaaatcctt
     6901 cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg gttgcttcac
     6961 cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg ctgccagacc
     7021 aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta gtgtcgtttt
     7081 gtgtagtgca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc attacctttt
     7141 atctaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt ctccatagga
     7201 gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg ggaaaagaca
     7261 taaggaaaag ccaatttgtc agacaaattg ttgaatgcac agcagattaa tccataagat
     7321 tagcctggaa atccttgttg tcttcggtac ccattcggga tgtcttcttt ttaaccagtc
     7381 aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccac aactcaaaga
     7441 tgtggaaaat gcgcgtcatt catttcgcta ttaattatca ctgtgctcat taattaacag
     7501 aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa atcatcactt
     7561 catcatgaat tcaaattcat cgattaatat caacaagata caaaaagcac tatcattaaa
     7621 attcattgca tttacattga tttcaccaat gaaatgtaaa aatatataaa cttgatgatt
     7681 taagcatttt cttataaccg ttcagacgtt attcttattt cagatcatcg tcagaattga
     7741 ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt aattatggtg
     7801 ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta tggcaaccgc
     7861 tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat ccaatctcta
     7921 ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca ctttccttcg
     7981 cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta tgaagagtgt
     8041 cggcgctacc cccatcgtta aagccattga tgttccaggt gaaaaactga actctctgga
     8101 agaactgttc caaaaaacga tggaagaata cgagcaacgt tcaagcactc tggcgcagtt
     8161 agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc gcgatctgga
     8221 aaaagaacag cagcatgatg gcctgttgct gcaaaccatt cttgatgaag tgcgcagtgc
     8281 gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg tcgtgtcaca
     8341 ccagctgcat tgatcataca tcggcgctaa tacattgcgc cgatgaagtt tttgagaaac
     8401 cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg tggggtaaac
     8461 ttgagttcaa tcagcgcgat ggctttttgg attgcccgca tggtgaccgg gtctgcggca
     8521 gcgggatgat gagtaaagtc gatattcttc agctgactgg acattttttc acgaacttca
     8581 acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg gcaagctacc
     8641 acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa ctcctgatag
     8701 cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac atccaccgtc
     8761 atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat attgcctggc
     8821 ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat tttgcgcagt
     8881 ttcatcgcgg cactccgacc atttgttata caggtgttat tgtctttgcg cttataatat
     8941 gacaaccatc acaaaaatca atctttatgt gatacaaatc acataaatac ccctttaatg
     9001 ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag atggcaagaa
     9061 atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc gtaaaatagt
     9121 gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct ttcattaata
     9181 acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga cgcggcaaca
     9241 ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac cgatcgttat
     9301 gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca cgcgatcccc
     9361 tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt gaatacggaa
     9421 ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta aatattttaa
     9481 tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat tcccattcgt
     9541 atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa caaattatag
     9601 tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa ccagaaatga
     9661 ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt tatcagcaaa
     9721 tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg
     9781 cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat accggcaatt
     9841 taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt gatgaattat
     9901 tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa ctggctcatg
     9961 ctgcaatgac caatcaggac tacccaacat ttaatttcct gcagtggtat gtttctgagc
    10021 agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg gcaggcaaaa
    10081 gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca caaaactaat
    10141 gctctccggc ggcaggtttc tgccgccgtt tcaaatcagt gacggcaaat cttactctga
    10201 tcggtcgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag aaacgctacc
    10261 agctctcccg ctgtcacccc ttctgccgag cacgtgtgaa aacgtgcctg ttcaccaaaa
    10321 cgcgctttaa tcgcagcttc cagactggca tgcgtatatt gctcgcctga ttcaatcatc
    10381 atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa aaagcctagc
    10441 gtagcgatta ccgcttatga agactttgcg ccagcgcagg actgaatgct tttattgtac
    10501 atttatattt acacaatatg taacgtcggt ttgacgaagc agccgttatg ccttaacctg
    10561 cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa aacagaaccc
    10621 tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg gcaatgccgc
    10681 tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg ctttgggcgt
    10741 tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg gcagataccg
    10801 gtctgggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg acgggcttca
    10861 gtatgatgtt cttaatgtat gctctgactg cagcatacat cagcggtgcc ggtgaattgt
    10921 tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt
    10981 tgttcacttt tgttgccggt ggcgtggttt gtgttggaac atcgctggtc gatttattta
    11041 accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta ttgctgctac
    11101 cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg gcactgtctg
    11161 caatcccggt gatttttacg tcgtttggtt ttcacggtag cgtgccgagt attgtcagct
    11221 atatggatgg caacattcgt aagctacgct gggtgtttat aatcggtagt gccatccccc
    11281 tggtggcata tattttctgg caggtggcga cgcttggcag cattgattca acaaccttta
    11341 tgggattgct ggctaatcat gctggattaa acgggctgtt acaggcgtta cgcgaaatgg
    11401 tggcctctcc gcatgttgag ctggcggtgc atttatttgc tgatttagcc ctcgccacgt
    11461 catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttgttt cagcgttcaa
    11521 ataccgttgg tggacgattg caaactggtg caattacctt tctgccgccg ttggcgtttg
    11581 cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg
    11641 tactggcatt gattatccct tcactattga cctggcaaag cagaaagcac aatcctcagg
    11701 cgggttaccg ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg
    11761 ctgtgattgg cgtgcaattt ttgattgcgg caggcttgtt accagaagtg gggtgatcag
    11821 atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatg tgacctctta
    11881 atgcaggcag cactgcttaa atttcttacc actgccgcac ggacaaggat cgttacgccc
    11941 cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat gcgccatcca
    12001 gtatgcatgt aaatcaagcg ccgccagtcg aatggcatct acgctctctt caaacgcttc
    12061 tggcgacatt ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc
    12121 cagcgctggt tttaaagaat cgggcaacgt tgaccagtca gaaagtgcca cgccccgcat
    12181 atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt ctcgcaagcc
    12241 gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat cggccatatg
    12301 ttgaaaagcc aggttcataa agcgcgtcat ctctttctct gacgcccaac gcggcacata
    12361 gtcagcccca ccccacacgg caaccagcca ctgttctggt tcaatctctt gcggagaact
    12421 caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt ggtcagtgtt
    12481 gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg gtcccgtttt
    12541 catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc cggtccgtat
    12601 cagagatact ttttgagtgg ctttgccggt gattaaaaat taaggagggt gtaacgacaa
    12661 gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat tactttgatt
    12721 ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag cgcgaacgcc
    12781 gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg aagaaagtgg
    12841 aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca taaccgcttt
    12901 gttaacgcgc caaattcttc aggcctttca gccagacatc cgcttgacgc cgatgtcttt
    12961 taaactggag cgggaaacga gactcgaact cgagaccccg accttggcaa ggtcgtgctc
    13021 taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga ttttattatc
    13081 gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc gcgatgagtc
    13141 aacgatattt ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac gatcactgat
    13201 caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga gtcagtaccg
    13261 cagccacaaa gaaaagtgca ataccgacgt actcaaccca aatgttcgga cgccacagca
    13321 gccaggccaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag gagacggcca
    13381 cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata ataatttcac
    13441 gggcgatcat cgttgccgcc ggtaatgtca cccaccagct gtgataatgc tcggttacca
    13501 gcaccatggc gatagccacg agaactttat ctgccacagg gtcaaggaaa gcaccaaacc
    13561 gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga
    13621 cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa aagaccaata
    13681 caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata ttaaattgca
    13741 taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac agagacatca
    13801 atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg gcacttttgc
    13861 aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat
    13921 ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat ttttgacctt
    13981 cgcccgtttt ttacggtgcc cgccaatcgc gtgatcatgt gattcatcgc gaatatgctg
    14041 gataacatgc agcgcgggtg aatctggcgg caaactaaat ccctcacctt ccggctcaaa
    14101 gaacagcgtt tccagtccag ccttacgatc tgctcctttg gcaacgccaa gtagcagcgg
    14161 atgattttta tcccatgaga catccagttc ggcgaagaca tttttcgcct gcgcaagctg
    14221 gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa tggctttacc
    14281 ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg tgatgcctgt
    14341 aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca ccacacagga
    14401 agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc gcttcacttc
    14461 cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag attgctgcga
    14521 aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat aacgcgccct
    14581 atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg aaagggaatc
    14641 ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg gtaaggtgcg
    14701 catctggctg ccttgtaaat agaactgacc tacgaaggtt tctaccacct cgctcagttc
    14761 cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc cctgacgaat
    14821 gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa cgtcaaggtc
    14881 gtcgccggta ttggaaacaa actgtttttc ggtgacgcgt cgcaccgcct gaatttggtc
    14941 gcgaatacat gccgcttctt caaactccag attctggctg gcagtttcca tacggctaat
    15001 gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat actcgacctg
    15061 ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac agcgccctat
    15121 ctggtattgc agacacggac gcgagcgatt gcgataaaca ctattttcgc actggcgaat
    15181 ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg
    15241 gccgaaatat tcacctttgg catgctttgc accacgatgc atcgccagac gcgggtgggt
    15301 atcgccactc aggaagataa aaggataaga tttatcatcg cgtagcaaaa cgttgtaacg
    15361 cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt ctgtatgcgt
    15421 aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc gcgaagcgag
    15481 gttgctgcgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc cgacatagat
    15541 aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta cggtttttaa
    15601 aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc attacacaga
    15661 ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag tttactgaac
    15721 atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc tgagatctca
    15781 ttgaccttct ggcccttagt gatcatcagc ataatctgca attcacgttc agacaaactg
    15841 gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg ttgagcgata
    15901 tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac gacttcctgc
    15961 ggagccgcgc ctttgctgag gtagccagca gcacctgcct gcatgacttt cgctggtaaa
    16021 gggttttctg tatggaccgt aagcatgatg attttaacat cagctgtgga acgcgcgatt
    16081 ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag caccacgtca
    16141 acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc tccggcgact
    16201 tttataccct taatatcttc cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca
    16261 tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac gctactgata
    16321 gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa aatggtgggt
    16381 attcgatttt tctctcgttt ttgtcaattc gattgtccac agttatggtc acgcccgtca
    16441 ccaacggctt accggatcgt ttcacagtaa gccctacgat gaaatgtgac aaaaattgac
    16501 tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta acccgaacaa
    16561 tacgggcaaa aaacattcat gataaaaata tttattggtc attatattaa cgtattttat
    16621 agcactgccg atatcacgct caaaaaacaa ccactgctat ttttagcaaa gcttatggta
    16681 tactccgccg ccttaacatt tttcaccaca aattttcatt gcaacatgac gaggaaaata
    16741 aatgagtacg cctgattttt ctactgccga gaataatcaa gaactagcaa atgaagtctc
    16801 ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg acgcgggccg
    16861 cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc aggctgcagt
    16921 attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata aaaccggctg
    16981 agagcgtgcc tttcagccgg ttattgacaa aatcatcgct atctctgctg atgtcttccc
    17041 ttccgccgtc catttcagaa tttctttttc gcgcttgctg aaattcatct ctggcgtcat
    17101 cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca gtaactgcat
    17161 ttttaattgc agttcatcac taagaatggg tatttcgcgc gtgctgcaac gggaaaagga
    17221 caaaaagccc agcgcccgat ttggcagcat taaatactga gtgacaccgc ggcgtaaacc
    17281 atgtgcgtgc gcggcttccc ataatggctg tgcttcgctg aataagtcat cattccacat
    17341 taaatggccc tgactaaagt tttcagggtt cagcacagga tcaattgcga gaaaattttt
    17401 tgcctgataa taactaaccc acgactcagg gtaattggtg tagaaagcca ctttaggtcg
    17461 cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca gctgctgagc
    17521 ctgaagctct atttcatggt aggcctcttc tgcggcctcc atcctctgaa aacgcaacag
    17581 catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca acgcccctga
    17641 gagtgagtat catttataat gataattcaa actatcacat aaataagatt tatatataat
    17701 ttatattatt caggcaatga attacttttg caagccatcg cattctctta tgttattaat
    17761 gagttatgct gatttgttaa gcagttttat cacgcttgaa atggcgtccc gccccgacag
    17821 gtgaatcgtc ggggctgatt ttttcttatt attgcagcag aagcttctcg aggaactggc
    17881 gggtgcgagg ctgctgggga tcggcaaata acgcttttgc tgccccctgc tcgactatcc
    17941 gcccctggtc cataaagatc gcccggtccg caacatcccg ggcaaagctc atttcgtgtg
    18001 tcacaatcac catcgtgcgc ttttcctgcg ccagctggcg gatggtgttc aggacttcgc
    18061 ccaccagctc tggatccagc gccgacgttg gctcgtcaaa caaaatcacc tcaggacgca
    18121 ttgccagtgc acgcgcaatc gcaacacgct gctgttgacc gccagacaaa cgacgtggat
    18181 agctggtttc tttaccggcc agcccaactt tcgccagcag ctcgcgagcg cgcgccgtgg
    18241 cctcttcttt cggttcgcct ttgacgatca ccggcccttc aataatgttt tccagcaccg
    18301 tacgatgcgg aaacaaatta aagttctgga agacaaaccc gacgtgctga cgcaactggc
    18361 gaatcagcga tttttgctga cttaatgaac gtgcagtatc aatagtgata tcgccaacgg
    18421 tgatcgttcc cgcttccggt tgttccagca gatttatgct acgtagcaat gtggttttgc
    18481 cggaaccact cggaccgata attgccacca cttcgccagg ctttacctcg aggtcgatac
    18541 cgtgcagcac cgtctgaccg tggaattttt tcaccaggtt cttaacttca atggcactca
    18601 ttttggttct ctctcctggc gattaagttg attctcaaaa tggttctgca acgtcgataa
    18661 cacagtcgcc atgatccagt agatgagcga agccgccagg tacatggtga acacctccag
    18721 cgtgcgcgag gtaatcaact gcgcctgacg gaacagctcc ggcacctgga ttgtcgcggc
    18781 cagcgacgta tcttttacca gactgataaa gctgttcgac agcggcggca acgccacccg
    18841 cgccgcctgt ggcaaaatcg cgcgacgcat ggtctgccac ggcgtcatac caatactggc
    18901 agcggcttcc cactgacctt tatcaataga agaaatggcg gcgcgcagcg tttcggcggc
    18961 ataggcggca gtgttcagcg acagaccaat cattgctgac ggaatgggat ctaattcgat
    19021 accaaactgc ggcaggccgt aatagatcat aaacagttgt gcgatgagcg gcgtaccacg
    19081 aaaaatagag atataaaaac gcgccagcca gcgcacgggc cagattggcg acaggcgcat
    19141 cagcgcgaga ataaacccga gtagtaagcc aaaaaacatg ccgccgatac tgagttgcag
    19201 ggtataccca gcccctttca acaggaacgg caaggaatca ataaccagtt gtatactttc
    19261 ttgcatgagc gttcccggag ctaaacgtgg ggatgatagg cgaacagcgc aggcgcgccg
    19321 ccggtatgaa taaacagaat cggcccttca tctttgaagc gtttctgact gataccgtca
    19381 atcagccccg ccatcgcttt tccggtatac acaggatcaa gcagaatgcc ttccagacgc
    19441 gccagcaatt tcactgcttc catgccttcg tcgttcggca cgccgtagcc aggtgcaaaa
    19501 tagtcatccc agagtaaaat ttccgctgat gcggtcagct ccagttcttt cgcaatcgcc
    19561 tgttgtaggt taaccacttt cggcaattga tcggcaacgg aacgcgacac ggtcacgcca
    19621 atcagttcgc tttcaggcat caggtgttcc agcccaacag ccagtccggc gtgagttccg
    19681 gcactgcccg atgcgactac caccgacgaa atattaaccg ccccttcaca ctgttgcgcg
    19741 atttccagcg cactctccac ataacctagc gcgccaagag cattagaacc gccaaccgga
    19801 atgacatacg ggcgaaagcc ttgtgcttcg actcgcgtcg ccagttcttc cagttgggca
    19861 ttgggatcgg tcagtgcgtc gcacatttca atctgggtat tgaacagatc caacaacaaa
    19921 cgattgccgt tggttaaata gttttctgcg gttgtgccaa taggattttc cagcagcgcc
    19981 acgcagtgca gaccgagttt cgccgcgact gcggcagtct ggcgcacatg gttagactgg
    20041 atcgcgccgg cagtaatcag cgtatcggca ccttcgcgca gggcatctgc cgcgagaaat
    20101 tccagcttac gtaatttatt gccgcccatt gccatggggg tgacctcatc ccgtttgatg
    20161 aaaatttccc gtccaagata atcagaaaag cgcggcagat attcgagcgg cgttggcgcg
    20221 ccgataaact ccaaccgtgg aaaacgggtt aaattatgca gtggcataac agcctccgat
    20281 gtgtgttgtt gtgattttct tattatgcac gctgaaaacg cgtaaataaa aaaggcgcta
    20341 gtgaaagcgc ccttttttgt cattatgctg attatttggt cacatcagca ccaaaccatt
    20401 tttcggaaag agcttgcaga gtgccgtctt tttgcatttc cgcaattgca tcattcaccg
    20461 ctttcagcag gtcttcattt cctttacgca gcgccacgcc agactcctga cgggagaatg
    20521 cttcaccggt tactgccagc gtatcgttgg ttttcttcac cagatccagc gccgccagac
    20581 gatcaacgag gatcgcatcg atacgcccta cgcgcagatc ctgatatttg gtcgggtcat
    20641 catcataggt acgcacatcg acaccctgaa cattctgccg cagccactct tcatagttgg
    20701 tgcccagacc gacacccact tttttgcctt tcagatcagc agccgtttta atggtgcctt
    20761 cgttaccttt tttcaccagc gcctgaatac cggaaatggt gtaaggggta gagaaatcgt
    20821 attttttctt gcgctcgtca gaaatggtga cctgattaat caccacatca atgcgtttag
    20881 agtccagcga cgccagcata ccgtcccatt tggtcggttt tagtgacgcc tcaacgccaa
    20941 gatgttttgc cagctgttgg gcaaattcca cttcaaaacc ggttaatttg ccgtcatccc
    21001 cctgaaaact gaacggcgga taagtgcctt ccagccctac cagcagcgtg ccgctctctt
    21061 tgactttatt aagcagacct tcatctgcaa aacttttaac gctcatgccc gcaaccagcg
    21121 ccacggccat cacacccatc aatgcctgac gtcccagata tgctaatttc atattcaccc
    21181 cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca aaaacaactc
    21241 cgctacatct tattcttatt taatatatat cagaagcagg caggctggag gatttctgca
    21301 caagtccggt acaggtttgg cgctgatagt gttgatactt ccgtaatgcg atgcggtaat
    21361 tgttggtgct ggtggtgggc agccacgggg cgaggatttc atcaaggaaa ccgtcctgca
    21421 gctgatcgag ggaaatattt tgctcatgta gatgattacc caaacggcgc agacgaacga
    21481 catattcacg caccgtacct gggctcattt cagtttgctc aaaaagaaat ttcttaaagc
    21541 cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa aatcggcaca
    21601 atgccgccca tgattgttgt tccgtttgcc agcctttttc atcaagcagc gtgtccaggc
    21661 gggaaatctc gattttattc actattttgc cgtcgcgaac taaggtaatg cgatcgagta
    21721 atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga tagcggctta
    21781 atggccgtct tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aagttaaagt
    21841 gcggcattta ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc
    21901 tgtgtaactg actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat
    21961 tcagctcttc ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga
    22021 tggcttccat cacccgctgg cgcagattac tgtccagcag ttgttgtagc gggttttctc
    22081 gctgatgatc atcagtaacc agttcgatgc tatcgccgtg ctcttcacgc cactcatcgt
    22141 aggagaagag ctggctgtta ttggtgtcga gcagcatttg acgataatcg gcaatatcga
    22201 tccctaaacg ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt
    22261 gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac
    22321 ggctgcgaag ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg
    22381 ccgttccttg tagggcgtca tagcgttcga cagcattaag taacccaatg ccgcccgcct
    22441 gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt
    22501 gacgcaccag cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg
    22561 tatagagtga attcacgata aacagccctg cgttatatga gttatcggca tgattatccg
    22621 tttctgcagg gtttttaatc ggacgattag tgggtgaaat gaggggttat ttgggggtta
    22681 cgcaaaaagc cgaatgtaaa aaaccccgcc ggtggcgggg tttgagaggc attcagacga
    22741 ttaaccctgc agcagagaca gaacctgctg cggtacctgg ttggcttttg ccagcacgga
    22801 gttaccggcc tgctggataa tctgcgcttt cgacatgttg gacacttcgg tcgcatagtc
    22861 ggcgtcctga atacgggact gcgcttcaga caggttggta gtggtgttgt tcaggttggt
    22921 gactgcggaa tccaaacggt tttgcaccgc acccagggag gagcggaatt tgtcgatgga
    22981 gctgatggct tcatccagag ctttcagggg atcggctgtc gtagctgctt ttgtttcagc
    23041 atctgtcgtc aatttaccat cagcatcttt ataaactgcc ttaccgctgc cgttagtcac
    23101 tgaaccatca tcctgtaggt aaaaatctac agtatcagat gtggtcaacg cctcgttgtt
    23161 atcaacataa accgaatcaa caccttttgt tacagcgcca gttgtgccgt tgacgctata
    23221 acctgcagta gtgaaatcaa cattgtttcc cgaggttgat acagctttat cgaaagctgc
    23281 ctgatcaact tttgcacctg ttactgaata agtaatagtg tccggcgtag tactattacc
    23341 ggtaaaggag atagaggtac cgccaatgtc aatggttgtc gctttggtat tattggctgc
    23401 aagtgtggac agactagcct cagtcaaaga agcaccagag ccgtttttcg ttaagcgccc
    23461 agttgtatca atgtagagtt tatctccatt gcttgccgta attttaccat cactgctgat
    23521 ctgaacatcc tgtgcagaac catcgatagt aatagtgccc ttagcggtat caccaactcc
    23581 cggggtcaaa tatttctgaa catcggcagc tgaagccgtt gtggtatcaa aagagtatga
    23641 cttacttgca ctgtcatact tgtaattcgt tgcactagcc gccgttccga agccgttatt
    23701 aatggttgcc tgaacagtat caccatcact aactccagcc agcaaatcag acgctttagc
    23761 agcatcgtaa cccgcactca cagtatattt gttgccgacc actgttgcat tagcagctac
    23821 caagtcagct ttagatgcag cagtattagc cacagccccg ccaccattca cattaaaccc
    23881 attcagcccc agcgtatcag agtcaatttt cttcagatca atagtgatag tctggccgtc
    23941 attcgcacca acctgaattt tcatcgaacc gtcttttgcc agtacgttca cgccgttgaa
    24001 ctgggtctga ccggatacgc ggtcaatttc gtcgagacgg gatttgattt cgtcctgaat
    24061 ggagtccaga tccgaatcag agttagtccc ggtagaagcc tgaaccgtca gctcacggat
    24121 acgctgtaag ttgttgttga tttcggacag cgcgccttca gtggtctgtg caacagaaat
    24181 accgtcgttg gcgttacgtg cagcctgagt caggccttta atgttagaag taaaacggtt
    24241 agcaatcgcc tgacccgcgg cgtcatcctt cgcgctgtta atacgcaagc cagaagacag
    24301 acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt gagtgatcag
    24361 cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat tgcaagtcgt
    24421 tgattacgtt ttgggtttct acccgtcggc tcaatcgccg tcaaccctgt tatcgtctgt
    24481 cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt cgacgaaata
    24541 ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta aactttgcgc
    24601 aattcaggcc gataaccccg gtattcgttt tacgtgtcga aagataaaag gaaatcgcat
    24661 ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa tccttgatag
    24721 cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca aatcagcaat cgtcgtttac
    24781 cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc agaccgccaa
    24841 tactgcattg tctaaagccg atcttttttc cgccaccagc accaccagca gcaccaccgc
    24901 gttcagtgcc accaccgcgg gtaacgccat cgccgggaaa tacaccatca gcgtcaccca
    24961 tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga gatgatacga aaacggcgat
    25021 cgccaccagc gacagcaaac tcaccattca acaaggcgac gacaaagatc cgattaccat
    25081 tgatatcagc gcggctaact catcgttaag cgggatccgt gatgccatca acaacgcaaa
    25141 agcgggcgta agtgcgagca tcattaacgt gggtaacggt gaatatcgtc tgtcagtcac
    25201 atcaaatgac accggccttg ataatgcgat gacactctcg gtcagcggtg atgatgcgct
    25261 acaaagtttt atgggctatg acgccagtgc cagcagcaac ggtatggagg tctcggtcgc
    25321 cgcccagaat gcgcagctga cggtcaacaa cgtcgccatc gagaacagca gcaacactat
    25381 cagcgacgcg ctggaaaaca tcaccctgaa cctgaacgat gtcaccacgg gcaaccagac
    25441 gctaaccatc actcaggaca cctccaaagc gcaaacggcg attaaagact gggtgaatgc
    25501 ctataactcg ctaatagata ccttcagcag cctgaccaaa tacaccgccg tagatgcggg
    25561 agctgatagc cagagttcta gcaatggcgc actgctcggc gactccacgc tgcggacgat
    25621 tcagacgcag ttgaagtcga tgctgagtaa taccgtcagt tcttccagct ataaaacgct
    25681 ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa ctggaactgg atgccgacaa
    25741 actcaccgct gcactgaaaa aagatgccag cggcgtaggt gcattgattg ttggcgatgg
    25801 taaaaaaacc ggcatcacaa ccaccatcgg cagcaacctg accagttggc tttcgacaac
    25861 gggcattatt aaagccgcta ccgatggcgt tagtaaaacc ctgaataagt taactaaaga
    25921 ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg cgctacaaag aacaatttac
    25981 ccaactggac gttttaatga cctcgttaaa cagcaccagc agctacttaa cgcagcagtt
    26041 cgaaaacaac agtaattcca agtaatcatt attcatcggg agacaggtca tgtacgcggc
    26101 aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc accgtaatga gcgccagcca
    26161 acagcagttg gtcaccatgc tatttgatgg agtgctgagc gcactggtta gagcgcgact
    26221 gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag cgatcaacat
    26281 cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaat taacccaaaa
    26341 cttaattgct ctttatagct atatggtcag gcgtttgctg caagccaatt tacgcaacga
    26401 tgtctccgca gtcgaagagg tggaagcatt aatgcgtaat attgccgatg tctggaaaga
    26461 gtctttactc tccccttctt cgattcagga cccagtctga tgaacaatgc accgcatttg
    26521 tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct ggcaacggaa
    26581 gaacaatggg acgaactcat cgctagcgaa atggcgtatg tgaatgcggt gcaggagatc
    26641 gcacacctga ctgaagaggt tgccccgtcc actacgatgc aggagcagct ccgcccgatg
    26701 ctgcacctga ttctcgacaa cgaaagcaag gtaaagcagt tattacagat tcggatggat
    26761 gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag tgcctatggc
    26821 gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctt aatgagccga
    26881 tggctcgcga ataatccgat tacggctacg cttctaatat tccccttgaa tggagtcgaa
    26941 gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa ggcggtaagc
    27001 tctggcctga actggccgag cgcgcagacg gttttaatga tattggtatc aatatggtct
    27061 ggttgccgcc cgcctataaa ggcgcatcgg gcggctattc ggtcggctac gactcctatg
    27121 atttatttga tctaggtgag tttgatcaga aaggcagcat ccctactaaa tatggtgata
    27181 aagcacaact gctggccgcc attgacgccc tgaaacgtaa tgacattgcg gtgctgctgg
    27241 acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc gtgcagcgtg
    27301 taaatgctga tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa ggctggacgc
    27361 gttacacctt ccccgctcgt gccgggcaat actcgcagtt tatctgggat ttcaaatgtt
    27421 ttagcggtat cgaccatatc gaacaccctg atgaagatgg catttttaaa attgttaacg
    27481 actataccgg agaaggctgg aacgatcagg ttgatgatga actcggtaat ttcgattatc
    27541 tgatgggtga gaatatcgat tttcgcaatc atgccgtgac ggaagagatt aaatactggg
    27601 cgcgctgggt gatggaacaa acgcaatgcg acggctttcg tcttgatgcg gtcaaacata
    27661 ttccggcatg gttttataaa gagtggatcg aacacgtaca ggaagttgcg ccaaagccgc
    27721 tgtttattgt ggctgaatac tggtcgcatg aagtcgataa actgcaaacg tatattgatc
    27781 aggtggaagg caaaaccatg ctgtttgatg cgccgctgca gatgaaattc catgaagcat
    27841 cgcgcatggg tcgcgactac gacatgacgc agatcttcac tgggacgctg gtggaagccg
    27901 atccttttca cgccgtaacc ctcgttgcca accacgacac ccaaccgttg caagccctcg
    27961 aagcaccggt cgaaccgtgg tttaaaccgc tggcgtatgc cttaattttg ttgcgggaaa
    28021 atggcgttcc ttcggtgttc tatccggacc tctacggtgc acattacgaa gatgtcggtg
    28081 gtgacgggca aacctatccg atagatatgc caataatcga acagcttgat gagttaattc
    28141 tcgcccgtca gcgtttcgcc cacggtgtac agacattatt tttcgaccat ccgaactgca
    28201 ttgcctttag ccgcagtggc accgacgaat atcccggctg cgtggtggtc atgtcgaacg
    28261 gagatgatgg cgaaaaaacc attcatctgg gagagaatta cggcaataaa acctggcgtg
    28321 attttctggg gaaccggcaa gagagcgtag tgacagacga aaacggcgaa gcgaccttct
    28381 tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa atccatcccc
    28441 ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc tttatccagc
    28501 gcggcggcac actctgccgt cgggcgatcc acacgctcca tcgtcatacc atcgtactca
    28561 atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac gttagtgaga
    28621 tcatcagaca tcaatgtcaa cttacccggc acggcaatcc cccgctgcca ctgacggcaa
    28681 tccaaggtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg actgaccagc
    28741 gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc gggggttttc
    28801 acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag cgcgcctgca
    28861 attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt ggcattaaag
    28921 ctctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta ttatttttgt
    28981 gaattattac cccgaaaata aatttgcatg gtgtatgatt cgcggctctt gtttcaaacc
    29041 ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc tggttgatta
    29101 aattctgggc acccatccct gcggtcatcg cggcgggtat tctctccact tactattttg
    29161 gcattactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc ggtcagctcc
    29221 tgcagctgtt tggcgtccat gctgaagagt ggggttattt taaaattatc catctggaag
    29281 gatcgccatt aacccgcatc gacggcatga tgatcctcgg tatgtttggc ggctgctttg
    29341 ctgctgcgct gtgggccaac aatgtcaaac tacgcatgcc acgcagccgt atccgcatta
    29401 tgcaggccat cattggcggc attatcgccg gttttggcgc gcgtctggca atgggctgta
    29461 acctggcggc gttctttacc ggcattcctc agttctcgct gcatgcctgg ttctttgcca
    29521 tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc atcttccgta
    29581 ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa ccggatcagg
    29641 cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc tgggcgctgc
    29701 tcacggcgat gaaccaacca aaactggggc tggcaatgct gtttggcgtc ggctttggtt
    29761 tactgattga acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg tggatcaccg
    29821 gacgtaccca tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc atcgggatct
    29881 tcagttacgt acagttaggc gttgaaccta aaatcatgtg ggcgggacca aatgcggtaa
    29941 ttggtggttt actttttggt tttggcatcg tgctggcagg cggctgcgaa accggctgga
    30001 tgtaccgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc aacgtgatcg
    30061 gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc accgactggg
    30121 acaaaatcaa cctgctgaaa acttttggtc cgatgggagg cctgctggtg acatatttgc
    30181 tgctgtttgc tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc ccccgtgagg
    30241 caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc gccttgatat
    30301 ggtgggtgaa ccctgccctt atccggcagt cgcaaccctt gaggcgatgc cgcaattaaa
    30361 aaaaggtgag atcctggaag tggtgagcga ctgtccgcag tcgatcaata atattccact
    30421 ggatgcgcgt aatcacggct acacggtgct ggatattcag caagacgggc cgaccattcg
    30481 ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga acatcgttac
    30541 ctctgacaca tagattttac ccgccccctc tcctattctt ttcagtatga tctcaggagg
    30601 ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga ggattacctt gcgcttctcg
    30661 cggaagatat tgaacgcgat attccctacg atcccgaacc cattggcaga tacaatgtcg
    30721 cgccgggaac caaagttctt ctgctcagtg aacgtgatga acaccttcat ctggatccgg
    30781 ttttctgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac gcccgcgtag
    30841 aaactgcctc aaccagtcgc atgtttaaac cgctctggca acatggtcgg gcaatctgtt
    30901 ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa aaaaagcctt attttatcta
    30961 tcgcgctgac ggacaacctg tttttatggc cgcgataggt agcataccgt ttgagcgtgg
    31021 tgacgaagcc gaaggatttt tgatcgtcac tgcggcggca gatcaaggtc tggtagatat
    31081 tcatgaccga cgcccgctgg ttctgtcgcc agaagctgca cgggaatgga tgcggcaaga
    31141 cattggtggt aaagaagcct cagaaatcgc taccagaagc tgtgtgccag caaaccagtt
    31201 cacctggcac cctgtgtcgc gcacggtggg taatgtcaaa aaccagggcg cggagttaat
    31261 tcaacctgtt tgttgacatc ctccacgccc tgaatgacga ggacccctgc tacgttcagg
    31321 ctgtcgcctg aatcatttcg gtgggttcct gcttcaacga gcggcctgac tgcaccatct
    31381 ctccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagctcca tttacatcgg
    31441 cgttcgctgt atatccacat acctggcatc tgaatttact ttgtgacagg cgattttctt
    31501 tcgctgtatg accacagcac gcgcaacgct ggcttgtgta cgctggcgga acagcaagca
    31561 cctgaccgcc acgccagagc tgcttgtact caagctggcg gcgcatttca taccagccct
    31621 gatccagtat cgaacggttt aaacctgatt ttgcccggac attgcgcccc ggctggctga
    31681 ccgtacccgc tgctgacttt gacatgttgc tgaccttcaa atcctcaatg actatcatta
    31741 cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga
    31801 tacaggaatg cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga
    31861 ccttgcggct taactgtcgc tgaagtctcg ccagcttctt ctggtttttc tgaaaactgt
    31921 ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg ccagcatcca
    31981 gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct gcctgaatac
    32041 tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaaca cccgtgacct
    32101 gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg ctgttttcct
    32161 gagcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc tttttgaatc
    32221 aggggaacgc cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg tctttcagtg
    32281 actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg gcatttttcc
    32341 actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg gccttatgat
    32401 tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct gcgaagcgcc
    32461 tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc tgtagtcgtt
    32521 tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc ggaaggcact
    32581 gtgttttcct gatgcatgct caccgggtct ttgttaccaa
//
LOCUS       DAJVRP010000040        32125 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVRP010000040 DAJVRP010000000
VERSION     DAJVRP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.040"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OQN12_001903"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OQN12_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1654246.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OQN12_001904"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OQN12_001904"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1654247.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OQN12_001905"
     CDS             complement(3736..4092)
                     /locus_tag="OQN12_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1654248.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OQN12_001906"
     CDS             complement(4096..4512)
                     /locus_tag="OQN12_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1654249.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OQN12_001907"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OQN12_001907"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1654250.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OQN12_001908"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OQN12_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OQN12_001909"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OQN12_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1654251.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OQN12_001910"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OQN12_001910"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1654252.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OQN12_001911"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OQN12_001911"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1654253.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OQN12_001912"
     CDS             10521..11504
                     /locus_tag="OQN12_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1654254.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OQN12_001913"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OQN12_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1654255.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OQN12_001914"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OQN12_001914"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1654256.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OQN12_001915"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OQN12_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OQN12_001916"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OQN12_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1654257.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OQN12_001917"
     CDS             complement(15306..15515)
                     /locus_tag="OQN12_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1654258.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OQN12_001918"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OQN12_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1654259.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OQN12_001919"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OQN12_001919"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1654260.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OQN12_001920"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OQN12_001920"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1654261.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OQN12_001921"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OQN12_001921"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1654262.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OQN12_001922"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OQN12_001922"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1654263.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OQN12_001923"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OQN12_001923"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1654264.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OQN12_001924"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OQN12_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OQN12_001925"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OQN12_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCS1654265.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OQN12_001926"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OQN12_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCS1654266.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OQN12_001927"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OQN12_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCS1654267.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OQN12_001928"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OQN12_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCS1654268.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OQN12_001929"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OQN12_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCS1654269.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OQN12_001930"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OQN12_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCS1654270.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8172 a         8247 c         7782 g         7924 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctctctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgtctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gcctt
//
LOCUS       DAJVRP010000041        32066 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVRP010000041 DAJVRP010000000
VERSION     DAJVRP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.041"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..889
                     /gene="espL1"
                     /locus_tag="OQN12_001931"
                     /pseudo
     CDS             1..889
                     /gene="espL1"
                     /locus_tag="OQN12_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1061..1168
                     /locus_tag="OQN12_001932"
     CDS             1061..1168
                     /locus_tag="OQN12_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654271.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1221..1979)
                     /locus_tag="OQN12_001933"
     CDS             complement(1221..1979)
                     /locus_tag="OQN12_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1654272.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2266..3195
                     /gene="pfkB"
                     /locus_tag="OQN12_001934"
     CDS             2266..3195
                     /gene="pfkB"
                     /locus_tag="OQN12_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1654273.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3296..3586
                     /gene="ghoS"
                     /locus_tag="OQN12_001935"
     CDS             3296..3586
                     /gene="ghoS"
                     /locus_tag="OQN12_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1654274.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3692..4552
                     /locus_tag="OQN12_001936"
     CDS             3692..4552
                     /locus_tag="OQN12_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1654275.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4593..5129)
                     /locus_tag="OQN12_001937"
     CDS             complement(4593..5129)
                     /locus_tag="OQN12_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1654276.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5276..5944
                     /gene="hxpB"
                     /locus_tag="OQN12_001938"
     CDS             5276..5944
                     /gene="hxpB"
                     /locus_tag="OQN12_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS1654277.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6107..6697
                     /locus_tag="OQN12_001939"
     CDS             6107..6697
                     /locus_tag="OQN12_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1654278.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6830..8220
                     /gene="tcyP"
                     /locus_tag="OQN12_001940"
                     /pseudo
     CDS             6830..8220
                     /gene="tcyP"
                     /locus_tag="OQN12_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8224..9040)
                     /locus_tag="OQN12_001941"
                     /pseudo
     CDS             complement(8224..9040)
                     /locus_tag="OQN12_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9317..9580)
                     /gene="cedA"
                     /locus_tag="OQN12_001942"
     CDS             complement(9317..9580)
                     /gene="cedA"
                     /locus_tag="OQN12_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCS1654279.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9763..12024
                     /gene="katE"
                     /locus_tag="OQN12_001943"
     CDS             9763..12024
                     /gene="katE"
                     /locus_tag="OQN12_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCS1654280.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12071..12829)
                     /gene="chbG"
                     /locus_tag="OQN12_001944"
     CDS             complement(12071..12829)
                     /gene="chbG"
                     /locus_tag="OQN12_001944"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCS1654281.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12842..14194)
                     /gene="celF"
                     /locus_tag="OQN12_001945"
                     /gene_synonym="chbF"
     CDS             complement(12842..14194)
                     /gene="celF"
                     /locus_tag="OQN12_001945"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1654282.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14299..15141)
                     /gene="chbR"
                     /locus_tag="OQN12_001946"
                     /pseudo
     CDS             complement(14299..15141)
                     /gene="chbR"
                     /locus_tag="OQN12_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15149..15499)
                     /gene="chbA"
                     /locus_tag="OQN12_001947"
     CDS             complement(15149..15499)
                     /gene="chbA"
                     /locus_tag="OQN12_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCS1654283.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15558..16907)
                     /gene="chbC"
                     /locus_tag="OQN12_001948"
     CDS             complement(15558..16907)
                     /gene="chbC"
                     /locus_tag="OQN12_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCS1654284.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(16992..17312)
                     /gene="chbB"
                     /locus_tag="OQN12_001949"
     CDS             complement(16992..17312)
                     /gene="chbB"
                     /locus_tag="OQN12_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS1654285.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17611..17949)
                     /gene="osmE"
                     /locus_tag="OQN12_001950"
     CDS             complement(17611..17949)
                     /gene="osmE"
                     /locus_tag="OQN12_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1654286.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18151..18978
                     /gene="nadE"
                     /locus_tag="OQN12_001951"
     CDS             18151..18978
                     /gene="nadE"
                     /locus_tag="OQN12_001951"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1654287.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19208..20095
                     /gene="cho"
                     /locus_tag="OQN12_001952"
     CDS             19208..20095
                     /gene="cho"
                     /locus_tag="OQN12_001952"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1654288.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20055..20630)
                     /gene="ves"
                     /locus_tag="OQN12_001953"
     CDS             complement(20055..20630)
                     /gene="ves"
                     /locus_tag="OQN12_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1654289.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20833..21318)
                     /gene="spy"
                     /locus_tag="OQN12_001954"
     CDS             complement(20833..21318)
                     /gene="spy"
                     /locus_tag="OQN12_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1654290.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21648..22616)
                     /gene="astE"
                     /locus_tag="OQN12_001955"
     CDS             complement(21648..22616)
                     /gene="astE"
                     /locus_tag="OQN12_001955"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1654291.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22609..23942)
                     /gene="astB"
                     /locus_tag="OQN12_001956"
                     /pseudo
     CDS             complement(22609..23942)
                     /gene="astB"
                     /locus_tag="OQN12_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(23939..25417)
                     /gene="astD"
                     /locus_tag="OQN12_001957"
     CDS             complement(23939..25417)
                     /gene="astD"
                     /locus_tag="OQN12_001957"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCS1654292.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25414..26448)
                     /gene="astA"
                     /locus_tag="OQN12_001958"
     CDS             complement(25414..26448)
                     /gene="astA"
                     /locus_tag="OQN12_001958"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1654293.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26445..27665)
                     /gene="astC"
                     /locus_tag="OQN12_001959"
                     /pseudo
     CDS             complement(26445..27665)
                     /gene="astC"
                     /locus_tag="OQN12_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28111..28917
                     /gene="xthA"
                     /locus_tag="OQN12_001960"
     CDS             28111..28917
                     /gene="xthA"
                     /locus_tag="OQN12_001960"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1654294.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29084..29792
                     /locus_tag="OQN12_001961"
                     /pseudo
     CDS             29084..29792
                     /locus_tag="OQN12_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29797..30474
                     /gene="ydjY"
                     /locus_tag="OQN12_001962"
     CDS             29797..30474
                     /gene="ydjY"
                     /locus_tag="OQN12_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCS1654295.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30489..31196
                     /locus_tag="OQN12_001963"
     CDS             30489..31196
                     /locus_tag="OQN12_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1654296.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31196..31744
                     /locus_tag="OQN12_001964"
     CDS             31196..31744
                     /locus_tag="OQN12_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1654297.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31754..>32066
                     /locus_tag="OQN12_001965"
     CDS             31754..>32066
                     /locus_tag="OQN12_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1654298.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NF"
BASE COUNT         8230 a         8223 c         7595 g         8018 t
ORIGIN      
        1 ggagttccag gggctatttt cgcgggtatt gcatcacgaa tgccatttgc ccctatacat
       61 ccatcggcaa tgttgttaat aatggctaca ggccagactc aacagcttat tacattattc
      121 aaacagttgc ccatactccc tgaaaaagaa atcattgaaa taataactgc gcagaatagc
      181 gttggtacac ctgctttatt tttggctatg atgaacggac atactgacaa cgtgaaaata
      241 tttatgcaag aaattcagtc actggtagat aatcacatca ttcatgaaga taatctggtt
      301 aaattactgc aaactaaaag tgctaacgaa acacctggac tttatatctc catgttgtat
      361 ggattcgatg aaataatcga tatctttctg aatacattaa ccactccaat agcactaaga
      421 gcttttaaac aaaaaactgg tgatgagtat tttagccatg aaaatacatg atggtgagcc
      481 aggattatac gccgcaatgg aaaataatca ccctttgtgt gtcacacggt tcctctctaa
      541 aattaatggc atcgccttta aatacaagtt gagcaaagct aacatcatgg atttattaaa
      601 aggagctaca gcacagggaa cccctgcatt atacatcgcc atgagcaagg gtaatgaaga
      661 cgtcgtgtta tcttatatat cgacgctggg tgcttttgca aaaaaacatt cttttagtca
      721 acatcagtta tttacactat tggccgctaa aaatcatgac aacatgtcag ctgttcatat
      781 agccattcat cataagcatt ataaaactgt agaaacatat tatgctgcta taaatgtcat
      841 cagccaaagc ctgagtttta gtgctgatga aataaagacg tatttataac cagctattta
      901 cgatatatat cgatacataa ttttattttt ttcataaaac gcagaaacag tgaccatgtg
      961 cacaacgcca cccgacggct gtaataacaa aaaatccctt gatgcctgtc ccttttgtta
     1021 cactccgtta tcacgcacaa gagatatgca ggacactggt atgccgacta aacgctttga
     1081 taaaaaacac tggaagatgg tggtggtgct actggcaatc tgtggcgcta tgttgttgct
     1141 acgttgggca gcaatgattt ggggctgagc agtgacaaac gaagacagcc agaccgcata
     1201 acacggtccg gcagataatt ttacatgctg tagcccagcg ataaggttgt ccgacggtcg
     1261 gtatgttccg gtgcagactc tggaggttca gagttccatg tgacgttgta agccaccttc
     1321 agtccaaaat gttcattaat agcaacattt aatgcgcttt cggagttcag cgttgtgtct
     1381 tccgcgccaa agaccgaaac accctgcgta aatttagcgt tgtcagtcaa ctgccatgca
     1441 taagccccgg aggcatagcc cagcggctgg gtttcactgg cattgtcggt atatttgtcg
     1501 taacgcacac ctggaccgaa ttcaaagcgg aaactgtgta ccgggccatt caaaaactga
     1561 cgaccataac ccgcggtcaa cacatcgcgc tctcgatagc cgttataacg gtctgtcagc
     1621 cagcttgcct gaccaaataa atagtcataa tcagttaaat taaagcggct acgcccgccc
     1681 gccgcatatt tttctgaaga acgctcatca ttagaagaag tattactggc gttcccccac
     1741 agcgaccagg cagtggtttg tccataccag gtcatggtgg tgtcagccgt aagtgaggag
     1801 cttttcgtat tgcctgattg tgcaagatat cctgcgttca gattaccttc gaaaggtttt
     1861 ttagcgctgg caggatcgtc catgacagta aaaacggaat catcggcagc tgcattcagt
     1921 gacgcaaaca tgccccccgc caacataacg atggcaggaa ctgtcttcaa aagcttcatt
     1981 tatcaagagt ccgtacaaca aaaaaagaga ccatcgcggt cccggaaact ttcttaagga
     2041 tcaaagatta gcgtccctgg aaaggtaacg aattataaaa aggcgcgaat aacttagcaa
     2101 tgtattctta tttcattttt tgaataagca tgtggcgaaa acagattttt atttatatat
     2161 atttatctgc aaaattttaa ataaagctct aataaatcat attgttaatt tcttcacttt
     2221 ccgctgattc ggtgccagac tgaaatcagc ctataggagg aaatgatggt acgtatctat
     2281 acgttgacac ttgcgccctc tctcgatagc gcaacaatta ccccgcaaat ttatcccgaa
     2341 ggaaaactgc gctgtaccgc accggtgttc gaacccgggg gcggcggcat caacgtcgcc
     2401 cgcgccattg cccatcttgg aggcagtgcc acagcgatct tcccggcggg tggcgcgacc
     2461 ggcgaacacc tggtttcact gttggcggat gaaaatgtcc ccgtcgctac tgtagaagcc
     2521 aaagactgga cccggcagaa tttacacgta catgtggaag caagcggtga acagtatcgt
     2581 tttgttatgc caggcgcggc attaaatgaa gatgagtttc gccagcttga agagcaagtt
     2641 ctggaaattg aatccggggc catcctggtc ataagcggaa gcctgccgcc aggtgtgaag
     2701 ctggaaaaat taacccaact gatttccgct gcgcaaaacc aagggatccg ctgcatcatc
     2761 gacagttctg gcgaagcgtt aagtgcagca ctggcgattg gtaacatcga gttggttaag
     2821 cctaaccaaa aagaactcag tgcgctggtg aatcgcgaac tcacccagcc agacgatgtc
     2881 cgcaaagccg cgcaggaaat cgttaatagc ggcaaggcca aacgggttgt cgtttccctt
     2941 ggtccacaag gagcgctggg tgtcgatagt gaaaactgca ttcaggtggt gccaccaccg
     3001 gtgaaaagcc agagtaccgt tggcgctggt gacagcatgg tcggcgcgat gacactgaaa
     3061 ctggcagaaa atgcctctct tgaagagatg gtccgttttg gcgtagctgc ggggagtgca
     3121 gccacactca atcagggaac acgtctgtgc tcccatgacg atacgcaaaa aatttacgct
     3181 tacctttccc gctaacaaaa acattccccc agcattgggg gaatcaccac caacctgtcg
     3241 gcaacgcgtt tctccgacta tgctcaaaag tcatgtgata acaaaggggt gaactatggc
     3301 cagtggcgat cttgtccgtt atgtcataac cgtcatgttg catgaggata cattgactga
     3361 aattaacgag ttgaataatt acctgactcg cgacggtttt ttgctcacca tgacggatga
     3421 tgagggaaat atccatgagc tggggactaa cacttttgga cttatcagta cccaaagtga
     3481 agaagaaatt agagaactgg tttcggggct tacccaaagt gcaaccggca aagatcctga
     3541 aatcaccatc acgacctggg aggaatggaa tagcaacaga aaataaatgg tttttgggca
     3601 ataatcagtc tgtggtgtgc gttagctcgt gtttttacac cgcattcttg cgctaacctt
     3661 atgatctggc agacaacatg ggagagacat catgtggcag gcaatcagtc gtcttttgag
     3721 cgagcagtta ggtgaaggcg aaatcgaact gcgtaatgaa ctgcctggcg gagaagtcca
     3781 tgccgcatgg catttgcgct atgcaggaca tgactttttc gtcaaatgtg atgaaaggga
     3841 actgcttccc ggttttaccg ccgaagccga ccaactggag ttactgtcgc gtagtaaaac
     3901 cgtcaccgtg cctaaggttt gggcagtagg cgctgaccgt gactacagtt ttctggtgat
     3961 ggattatctc ccacctcgtc cgctggatgc gcatagcgca tttattcttg gtcagcaaat
     4021 tgcgcgttta catcaatgga gtgaccaacc acaatttggc ctcgattttg ataactcgct
     4081 ctccacaact ccacagccca acacctggca acgtcgctgg tcaacgtttt ttgctgaaca
     4141 acggattggc tggcagttgg aactggcagc agagaaaggg atcgctttcg gcaatatcga
     4201 cgccatcgtc gagcatattc agcagcgtct ggcctcgcat caaccgcagc cttctctgtt
     4261 gcacggcgat ttatggtccg gcaactgtgc gctgggtccg gatggcccgt acattttcga
     4321 ccctgcctgc gactggggtg accgagagtg cgacctagcg atgttaccgc tgcatactga
     4381 acagccgcca caaatctatg acggctatca gtcagtatcc ccgctacctg ccgatttcct
     4441 tgaacgtcaa ccggtttacc aactctacac gctgttaaat cgtgcaagat tatttggcgg
     4501 tcagcatttg gttattgctc agcagtcatt ggatagatta ttagcagcat gatatgggtt
     4561 gaggataatg gccgctccgt gcggcctttt gattaaataa acccaagcag agaaaagaac
     4621 acataacctg cagcaataca aataaccggc aggatatata gtgagaaaaa ctgcaggaaa
     4681 atagtatggt gcggaacaac aatacgggac tcaatttgtt cacgcgttag cccctcttcc
     4741 cctttggctt tttccagaat gagttgatct tcaacacctt cccgcaagaa gcgcgcctgg
     4801 cgactcatcc gggcaccgga atcctgcaac gccagtccga caaaaatcag gatgaaaatc
     4861 acccagaaca taacgttcac accaccatta aaattagggg tcggggagtt ataccagaac
     4921 agattcaaaa aaggcgtatt cgcctgcatc atatcgatca tgacatgagc aaagtcgagc
     4981 atgaccgcat taatgccttc ctggttttcc tgccgggtat tcataaattt cagcaaagaa
     5041 atcaatgtag agatcaatgc aggtataaaa atcacccaac ccaaaatcct tttcaaaaca
     5101 gcaatgcgtc cagcttgttg atacgtcatg agttctcctt gattaagacg cgtcgtttca
     5161 cttagtttac ctgtagatat ctgttttcgc ccattcttta aaggcgatat gataggcgct
     5221 taatcataag cacggcttaa taccttacac ataatgctct aaaggagagg ttgtaatgtc
     5281 aaccccgcgt cagattcttg ctgcaatttt tgatatggat ggattactta tcgactcaga
     5341 acctttatgg gatcgagccg aactggatgt gatggcaagc ctgggggtag atatctcccg
     5401 tcgtaacgag ctgccggaca ccttaggttt acgcatcgat atggtggtcg atctttggta
     5461 cgcccggcaa ccgtggaatg ggccaagccg tcaggaagta gtagaacggg ttattgcccg
     5521 tgccatttca ctggttgaag agacacgtcc attattacca ggcgtgcgcg aagctgttgc
     5581 gttatgcaaa gaacaaggtt tattggtggg actggcctcc gcgtcaccac tacatatgct
     5641 ggaaaaagtg ttgaccatgt ttgacttacg cgacagtttc gatgccctcg cctcggctga
     5701 aaaactgcct tacagcaagc cgcatccgca agtatatctc gactgcgcag caaaactggg
     5761 cgttgaccca ctgacctgcg tggcgctgga agactcggta aatggcatga tcgcttctaa
     5821 agcagcccgc atgcgttcca tcgtcgttcc tgcgccagaa gcgcaaaatg atccacgttt
     5881 tgtattagca aacgtcaaac tctcatcgct gacagaactc accgcaaaag accttctcgg
     5941 ttaatgacca ggggcagtga tcacgctgcc cctggttctt tatctgaatt tgcattcaac
     6001 tgacggatta atcgtcaatt taagagaaag agttacaccg tcaccacttc cgtgcactgt
     6061 ataaaaatcc tatactgtac gtatcgacag tttagtgagt tttatcatga cggcggaagg
     6121 tcaccttctc ttttctattg cttgtgcggt atttgccaaa aatgccgagc tgacgcccgt
     6181 gctggcacag ggtgactggt ggcatattgt cccttccgca atcctgacgt gtttgttacc
     6241 ggacatcgat cacccaaagt cgtttcttgg gcagcgatta aaatggatat caaaaccgat
     6301 cgcccgcgct tttgggcatc gtggttttac ccacagtctg ctggcggtat ttgcgctgct
     6361 ggcaaccttt taccttaagg ttccggaagg ctggttcatt ccggctgatg cgctacaagg
     6421 aatggtgctg ggttatttga gccacatact tgccgatatg ctgacacccg ccggtgttcc
     6481 cctgctctgg ccatgccgct ggcgtttccg cttgcctatc ctggttcccc aaaagggcaa
     6541 ccaactggaa cgttttatct gcatggcatt atttgtctgg tcggtatgga tgccccattc
     6601 attacccgag aacagcgctg ttcgttggtc atcgcaaatg atcaatacct tgcagatcca
     6661 gtttcatcgg cttattaagc atcaggttga atactaaaaa ggcaaaaatc acctttctgg
     6721 aataagcaat tccatttgaa tataagagcc agctcacagt tctgttaatc ttgcgccaac
     6781 actatgactg ctacgcagtg atagaaataa taagatcagg agaacgggga tgaactttcc
     6841 attaattgcg aacatcgtgg tgttcgttgt actgctgttt gcgctggctc agacccgcca
     6901 taaacagtgg agtctggcga aaaaagtgct ggtgggtctg gtgatgggtg tggtttttgg
     6961 ccttgccctg cataccattt atggttctga cagccaggta cttaaagatt ctgtacagtg
     7021 gtttaacatc gttggtaacg gctatgttca actgctgcaa atgatcgtta tgccgttagt
     7081 cttcgcctct attctgagcg cggttgcccg tctgcataac gcatctcagt taggcaaaat
     7141 cagttttctg accatcggta cgcttttgtt taccacgctg attgcggcgc tggtcggtgt
     7201 gctggtcacc aacctgtttg gtttgacggc tgaaggtctg gttcagggtg gtgcagaaac
     7261 tgcacgtctg aacgccattg aaagtaacta tgttggtaaa gtctctgatc agagcgttcc
     7321 gcagctggtc ttgtccttta tcccgaaaaa cccgtttgcc gatcttaccg gagccaatcc
     7381 gacgtcaatc atcagcgtgg taatttttgc cgcattcctc ggcgtagctg cattgaaact
     7441 gctgaaggat gatgcgccga aaggggaacg cgtcttaacc gctatcgata ccctacaaag
     7501 ctgggtgatg aaactggttc gccttgtcat gcagttgacc ccttacggcg ttctggctct
     7561 aatgaccaaa gtggttgcag gttctaactt gcaagacatc atcaaactgg gaagtttcgt
     7621 tgtcgcgtcc tacctcggtc tgctgattat gtttgctgtg catggcattc tgctgggcat
     7681 taatggcgtg agtccgctga agtacttccg taaggtatgg cctgtgctga cgtttgcctt
     7741 taccagccgt tccagtgctg cgtctatccc actgaatgtg gaagcacaaa cgcgtcgtct
     7801 gggcgttcct gaatccatcg ccagtttcgc cgcctctttc ggtgcaacca ttggtcagaa
     7861 cggctgcgcc ggtttgtatc cggcaatgct gcggtgatgg ttgcgcctac ggttggcatt
     7921 aacccgctgg acccgatgtg gattgcgacg ctggtcggta ttgttaccgt tagttccgca
     7981 ggcgttgccg gtgtcggtgg tggtgcaact ttcgccgcac tgattgtgct gcctgcgatg
     8041 ggcctgccag taaccctggt ggcgctgtta atctccgttg aaccgcttat cgacatgggc
     8101 cgtacggcgc taaacgttag tggctcgatg acagctggca cgctgaccag ccagtggctg
     8161 aagcaaaccg ataaagccat tctggatagc gaagacgacg ccgaactggc acaccgttaa
     8221 ttcttatgct ggcaacggtc cgttttgtat aggggccgtt gctttacttt ttaaatgttc
     8281 catcgcggcg atgcatactg actcatattt aaacagatcg tcagcgaaaa aaatgtccaa
     8341 cttatcaata cattcctggc tcaatgaatg tgcctgtagt gagaagctta aaacctcata
     8401 cccgagacgt tttctaagtg aactatctgg aatactcata atatcttcaa gcacaccact
     8461 tcccattccc ggaagattta gtggattaaa aagaagattg atattaagca ttgatattat
     8521 cgaaggaaac tttaggagac tctcattttt tttgaatcgc agcaaaaatg ctcccggcag
     8581 gatgtatata atttttttca tagtatatag tccttaaata atgaataacc aacctatttt
     8641 ttatattgat caaattcgtc attttttcgg gtcggaattc acaggtaaaa tccatcccaa
     8701 caaaattctc attaatatct cttatccgaa actcactgcc ataaagatta ttaattatca
     8761 ctctatattt aacattgtcc tgcagatacg tatcaaaact aagtgctggc gctcctagct
     8821 ttgaggcatc atagttaatg ttagacatat aggcaatcat acgattgaag ttcgactctg
     8881 ctatttcatc gcagtcacat tgacccgttg atataaagaa agtgagtgcc gccatggtag
     8941 actcaagcct ttctttaaaa gagtcgaaag ccaggctttt aatatttaaa tcaccataat
     9001 tactctgtat taagtttgta gaaaacatct cccgcctcat attgttaaca aaattattat
     9061 cttatttaaa tctaagtcat ttacaatata agtttaagag cgacgccaca ggatgaacta
     9121 tcaaaaataa ctcatcatga ttagcaaaac ttaaccattt taaaataaat aaataattaa
     9181 agaaaaaaga tcacttattt atagcaatag ctcgtcaaag gcagcttttt gttacaggtg
     9241 gtttgaatga atgtagcaac gaaatgcaga atttcaggtc ctgtaactcc cggcaaaacc
     9301 gggaggtatg taatccttac tcagtcactt ccccttcctg gcggatctga tttgcccaac
     9361 gttgggcaga ttcaggcaca gtaaacgccg gtgagcgcag aaatgactct cccatcagta
     9421 caaacgcaac atatttgcca cgcagcatcc agacatcacg aaacgaatcc atctttatcg
     9481 catgttctgg cggcgcgggt tccgtgcgtg ggacatagct aataatctgg cggttttgct
     9541 ggcggagcgg tttcttcatt actggcttca ctaaacgcat attaaaaatt agaaaaactg
     9601 tagtttagcc gatttagccc ctgtacgtcc cgctttgcgt gtatttcata acaccgtttc
     9661 cagaatagtc tccgaagcgg gatctggctg gtggtctata gttagagagt tttttgacca
     9721 aaacagcggc cctttcagta ataaattaag gagacgagtt caatgtcgca acataacgaa
     9781 aagaacccac atcagcacca gtcaccacta cacgattcca gcgaagcgaa accggggatg
     9841 gactcactgg cacctgagga cggatctcat cgtccagcgg ctgaaccaac accgccaggt
     9901 gcacaaccta ccgcccctgg gagcctgaaa gcccctgata cacgtaacga aaaacttaat
     9961 tctctggaag acgtacgcaa aggcagtgaa aattatgcgc tgaccactaa tcagggcgtg
    10021 cgcatcgccg atgatcaaaa ctcactgcgt gccggtaacc gtggtccaac gctgctggaa
    10081 gattttattc tgcgcgagaa aatcacccac tttgaccatg agcgcatccc ggaacgtatt
    10141 gttcatgcac gcggctcagc agctcacggt tatttccagc catataaaag cttaagcgat
    10201 atcaccaaag cggatttcct ctcagatccg aacaaaatca ccccagtatt tgttaggttc
    10261 tctaccgttc agggtggagc tggctctgcc gataccgtgc gtgatatccg tggttttgcc
    10321 accaagttct atactgaaga gggtattttt gacctcgttg gcaataacac gccaatcttc
    10381 tttatccagg atgcgcataa attccccgat tttgttcatg cggtaaaacc agaaccgcac
    10441 tgggcaattc cacaagggca aagtgcccac gatactttct gggattatgt ttctctgcaa
    10501 cctgaaactc tgcacaacgt gatgtgggcg atgtcggatc gcggcatccc ccgcagttac
    10561 cgcaccatgg aaggcttcgg tattcacacc ttccgcctga ttaatgccga agggaaggca
    10621 acgtttgtac gtttccactg gaaaccactg gcaggtaaag cctcactcgt ttgggatgaa
    10681 gcacaaaaac tcaccggacg tgacccggac ttccaccgcc gcgagttgtg ggaagccatt
    10741 gaagcaggcg attttccgga atacgaactg ggcttccagt tgattcctga agaagatgaa
    10801 ttcaagttcg acttcgatct tctcgatcca accaaactga tcccggaaga actggtgccc
    10861 gttcagcgtg tcggcaaaat ggtgctcaat cgcaatccgg ataacttctt tgccgaaaac
    10921 gaacaagcag cattccatcc aggtcatatt gttcctggtc tggacttcac caacgatccc
    10981 ctgttgcagg gacgtttgtt ctcctatacc gatacacaaa tcagtcgtct tggtgggccg
    11041 aatttccatg agattccgat taaccgcccg acctgccctt accataattt ccagcgtgac
    11101 ggcatgcatc gtatggggat cgacactaac ccggcgaatt atgaaccgaa ctcgatcaac
    11161 gataactggc cgcgcgaaac accaccgggg ccgaaacgcg gcggttttga atcataccaa
    11221 gagcgcgtgg aaggcaataa agttcgcgag cgcagcccat cgtttggcga atattattcc
    11281 catccgcgtc tgttctggct aagtcagacg ccattcgagc agcgccatat tgtcgatggt
    11341 ttcagttttg agttaagcaa agtcgttcgt ccgtatattc gtgagcgcgt tgttgaccag
    11401 ctggcgcata ttgatctcac tctggcccag gcggtggcga aaaatctcgg tatcgaactg
    11461 actgacgacc agctgaatat caccccacct ccggacgtca acggtctgaa aaaggatcca
    11521 tccttaagtt tgtacgccat tcctgacggt gatgtgaaag gtcgcgtggt agcgatttta
    11581 cttaatgatg aagtgagatc ggcagacctt ctggccattc tcaaggccat gaaggccaaa
    11641 ggcgttcatg ccaaactgct ctactcccga atgggtgaag tgactgcgga tgacggtacg
    11701 gtgttgccta tagccgctac ctttgccggt gcaccttcgc tgacggtcga tgcggtcatt
    11761 gtcccttgcg gcaatatcgc ggatatcgct gacaacggcg atgccaacta ctatctgatg
    11821 gaagcctaca aacaccttaa accgattgcg ctggcgggag acgcgcgcaa gtttaaagca
    11881 acaatcaagg tagctgacca gggtgaagaa gggattgtgg aagctgacag caccgacggt
    11941 agttttatgg atgaactgtt aacgctgatg gcagaacacc gcgtgtggtc acgcattcct
    12001 aagattgaca aaattccggc gtaaatatgg ataaggtcac gtcccctggg gagtgacctt
    12061 accaccttac ttacacatca agataactac ccaaccgata accacgctca gcaattgcat
    12121 atttcaacga cgctgatgtc agcacttcca gctccgttaa ccgtgggaaa cagtaagcac
    12181 tctgacggat agtattatcg ataaacgccg gatgacacat cacctccagc gaaggatcac
    12241 cccgatggct tgcgtcatcc agcacttgca ggaacagtgc ctcgctaatc tcttcaccat
    12301 aaaacgcgct actgaatccc tgcgaactgc gcagattaac cggtaaatca ccagcgttag
    12361 atagcggctg acggtcgatg cgcaacgcaa tcccctcttc agccgcaaac ctggcaacaa
    12421 tcgggaaaat ctgcgggaac atatgcacat gatgatggct atcaagatgc gtaggtttgc
    12481 gtccaaatag ctcaatgaaa cgcaaatact gactggcaag ctcctgagta atttcttcca
    12541 gcggtaaagc atcttcttct gccaactgcc agatccattt tcccagcaca ccatcgcggg
    12601 ttaaccccgg catagctgtc agtggcttgc ccatagtaag gacaaagtgc atccctatgg
    12661 ccagactcgg ttcatcacga ctcaactgca ccgcatggtc aatagcctgc ccattcacca
    12721 gcgccgtcgt cgatgtgaca atcccattgc gacaggcctc gataatgccg tagttctgac
    12781 ctttgcttaa gccaaaatca tcggcattaa caatcagtaa gcgttccata atcagcctcg
    12841 gttaatgtgc ttttttaagc tctgcgatgc agtcggcaaa atttggcagc catttctcgt
    12901 gcgccagaat catctcgcgt gccagcagct cagcatcgcg atcggaatgc accaacggac
    12961 taaggtttag cgccagtaac acatcgttaa attctccgct aagtgcggcg ttgctggcag
    13021 caatctcgaa gcctttaatg gtgtgaatca gccccatcac tttatcatcg aaatgcgtaa
    13081 tgcgcggatg tggcgtcgcg ccatcgtgcc ccagcttaca ggtcatttct accgcccagt
    13141 ctgccggaat attatcaata tgcccatgat gcgggatatt aacgtaatgt tcagcttgct
    13201 tgtcgttgta gatagcgttg atcacttcgc acgctgcatc agagtaataa gccccaccgc
    13261 gctgttccag ttctttcggc ttaactttca actccgggtt tttatacagc tcaaaaagtt
    13321 gtttctcgac tttctgtact acctgcgctc gtgcgccgcc tttgtagtat tcgcccattt
    13381 caatagccag catctctttt tgcttgaagt aataaagcaa ataagaacac ggcaacagat
    13441 tcagagaacg aattaagcct tcgctaaatg gcagatcgaa aatattttta acgccagata
    13501 cttttaactg cccggatgcc acaccatcaa gcaattcggc aaagcgcgac ttgccattta
    13561 ccagcacatc cttaatgaac accatatggt tgaggccaaa caaatcgata gataaatcat
    13621 cgctgtcttt cagcatcaga acatcgtgaa taaacatctt catgccgatc ggaatattac
    13681 acacgccgat aaaacgttta aatccggtat gacgataaac ggcttcagta accattcccg
    13741 ccgggttagt gaagttaatc acccatgcat tcggacaaag ttcttcgaca tcttttacga
    13801 tgtcaaaaat caccggaatg gtacgcagac ctttaaacaa accgcccgcg ccgttggttt
    13861 cctgaccaag ataaccatga cttaatggaa tacgttcatc cagttcacgc gccggtaatt
    13921 ggccaacgcg cagttgggta gtaacgaaat cagcatcttt caatgcttcg cggcaatcca
    13981 gcgttttata aagcttcatc gggacgccag cgttatcaat catccgttgg cagagatcga
    14041 aaataatatc cagtttagct ttgccaccct cgacatccac cagccataat tcgctgaccg
    14101 gcaattcgtg ataacgctta ataaatcctt ccagtaactc cggggtatag ctgctcccgc
    14161 caccaatagt gacgactttt aatttctggc tcataatttc tcccttcagt acagaatact
    14221 gatatctggc atatctgccc ccccggacat aaataatcca gcaacaggac agatatgtga
    14281 attgtcaggt ataacgactt actgattaaa ttcagtcaat ttcttacgat atctcttagg
    14341 cgtaaaggac gttaatttct taaacgtttt aataaacaaa ctcgggctac tataaccggc
    14401 ctcaaacgca atatccgtca cggaatagtt ggtcatttcc agttgttttt ttgcaaaatt
    14461 aatacggatc tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg
    14521 cgtcaaatat tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact
    14581 aaactgctct ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc
    14641 aatcacctgt tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc
    14701 aacaaactct tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata
    14761 ggtgagaaaa gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa
    14821 cggcaggtaa tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc
    14881 accaaactca taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg
    14941 ttccagtaac acgcgcttac cgttaatctc ctggaaataa cgcccggtta atactagagt
    15001 aaattcataa tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa
    15061 cacatggaag tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc
    15121 gttaatcact ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta
    15181 attcagtaat cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca
    15241 gcaccagact cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta
    15301 ccagatgcgc ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa
    15361 aatcgccctg cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt
    15421 tgatgataag ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa
    15481 tattatcgag atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac
    15541 cggcatcgat taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc
    15601 gcattttgtg ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca
    15661 aggttgaaga gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc
    15721 agaccggttg gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag
    15781 tacgctgcga gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc
    15841 accgggttca tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg
    15901 gacggcagcg ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata
    15961 gccaggatca ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc
    16021 ggcttggccc agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat
    16081 tgctgatagg tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg
    16141 gtcagcgcca gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag
    16201 gcccagccca ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc
    16261 tgatggaagt tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga
    16321 ataataaagc cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg
    16381 ggcagtttaa tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg
    16441 ccaataataa tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag
    16501 gcctcaccga cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc
    16561 gccagcgcat cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt
    16621 aaagacatta ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta
    16681 agtgtttcaa tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgacccctcc
    16741 ccaaagctta gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc
    16801 attaagcgaa taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact
    16861 gcaaaaggga ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct
    16921 taaaaaccgc aatttaaata ttacggtatt gatttatgaa ataactcttt gacgggaaaa
    16981 tttaaaataa attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg
    17041 cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt
    17101 aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg
    17161 gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact
    17221 tcatattttt ctgcctgtgc gcgcattttt gataccagta aagagataga catgcccgca
    17281 gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt
    17341 cagccagaca ctccgcaagc cttgacctgc ttccatgctc tgggtaactt gcgaaaccaa
    17401 catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt
    17461 tgaccttata aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa
    17521 ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc
    17581 cgttgttact caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta
    17641 ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa
    17701 gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag taccgcgagc
    17761 atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct
    17821 catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg tacgatcata
    17881 agctgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt
    17941 cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg
    18001 cgctttttca agcccgtgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt
    18061 taaccaccat tcagcccgct gtcgcttgtc gtttcagtag caacgggtta gctttaagga
    18121 agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg
    18181 ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt cgattttctg
    18241 aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt
    18301 caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgccaggaa
    18361 actcgcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggctgac
    18421 gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat
    18481 atcaagggcg cggtattggc tagcgagcag gcattgcggg aagcaggcat tgaactgagc
    18541 gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg
    18601 ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc
    18661 ttcactaaat atggtgacgg cggtacggat attaatccgc tgtatcgtct caacaaacgc
    18721 cagggtaaac agttactgac ggcattaggt tgcccggaac acctttataa gaaagcccca
    18781 actgccgatc ttgaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc
    18841 tatgacaata tcgacgacta tctggaaggg aaaaacctac ctgaacaggt cgccagaaca
    18901 atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattaa cgttttcgat
    18961 gatttctgga aaaagtaata atttgcacct attggattgt gcgaaaaaga gtaatttgtt
    19021 cacgccggat gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatacat
    19081 tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatca
    19141 acccgcctct tttttaatcg cctcccgcct gttacactgg atagataacc agcattcgga
    19201 gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta
    19261 tgaatatccc gaacatttac attcattcct taatgactta cccacccgac caggggtgta
    19321 tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg
    19381 cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg
    19441 gcgaattagc tggatctgca ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt
    19501 aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc
    19561 cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg
    19621 agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagag
    19681 catcgctgat gaacaaaaac tttgttatgg cctgttggga ctggaaccgt taagtcgcgg
    19741 tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag
    19801 ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcgag tggtgtgttg
    19861 gccttggcaa ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat
    19921 tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat
    19981 tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc
    20041 cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca
    20101 ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc atcgaaccag
    20161 cacgcgccct ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc gttaatcaca
    20221 aacaccaccc cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc
    20281 gccttacaga catccaggcg cgtcataata ttgaaatcca tcgacatctg ccctgccgtc
    20341 agtttcgctt ttaccacctg gtccgccgta aaggcaaaag gctgtaacgg ttttaaggta
    20401 tggttaaagc ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc
    20461 ctttccatgc cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca
    20521 cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca
    20581 gcgtttcgcc acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc
    20641 ggcccgttct tttcattgtt atcgttcagg taaccgtgga tgattatcgg caactcgggt
    20701 gatttacgtt agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc
    20761 gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt
    20821 ttggctgaaa gattattcag cagttgcagg cattttacct tttgccgctg gacgttctgt
    20881 cagacgcttc tcaaaattag cattaaattg ctttttctgt tccggcgtca ggatgttgta
    20941 aattttgttc tgggtttcca tgtgcgccag catgttagct ttgcgctgtt cttccatttt
    21001 tgcgatctgc gcttcagctt ttgctttatc gaaggtatcg ctggcaatga tgtcatgcat
    21061 tgcgcggcgt tcttccagcg gcggacgttt catctggtca cgctggcctt tcatgatttc
    21121 gtggatctgc tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca tgtcctgatg
    21181 cggaccgaac ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg ctgcggtagt
    21241 ggtgtctgcg gcatgggcca ggttagccgc gccaagagcc agggtagagg caacaaacag
    21301 tgcagttaat ttacgcatat tctatatcct tcctttcagt tatttattac ggctttctta
    21361 agtagcgtgc cgtgttgacg agattaactt tactgacttt agcgtcaatt aatcagagca
    21421 acggtaaaac aatgaaagtg taaaaaacac ttttgcgcca attatggaga aaaaaagaaa
    21481 atttgatgga gagtgatgag agaatattac aacacgatga ttttgcagag attatgaaga
    21541 actataccgg atgactggtg ataaataaag caaataacca ggattaatct gtattaattt
    21601 ataagaaagc aacttaatac ccgcagaatg atttctgcgg gtaagtatta gcttattttt
    21661 tcgagcatta atcccgcgcg taatcccaac gctaccaacg gattagggaa taacacatac
    21721 tctacatcat gggttacggt aaaacgttcc tctccgtcct gcgccagcaa tgttcctttc
    21781 tcaaacggca taaaattcag cgtgtcactt gccatatgca tttcgaagga cggcgagtgg
    21841 cgagtaattt gcgaaaccac ccgataacgg agcggcggtg ttctcacgat accgacactc
    21901 tcaccagata gcagcgcagc aattgcgctg gcagttactg caaactggcg aagatcgttt
    21961 tgcccaaagg gcaacgcttt gccaagttcc aacgtacaga ccagcgcgcc aaaatgtctg
    22021 acgctgaaat gggtaaacgt accaccaggc tcctgatgga acaccagcgc ctccagcccc
    22081 gccgcaccca gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg cggtaataca
    22141 ccgaactgcg gatgcaagga gccacgaatt gcggtatgta gatcaaggtg ccaacgcaca
    22201 gattctttgc cctggtcata aaaatcttcc aggcactgtt ccagttcgcg cgcccgacag
    22261 gtttctccgc tttcagcaaa tagctgccaa cgaccgccaa acattcggtt catatcgcta
    22321 tggcaataac gtttcccttg cttcagcgca ggaggattcc cgaggatcac cagcaaccgc
    22381 caacgtaacg ggatctcgcc gtgagatatc gcgccaagca acgcgtccag catttccaca
    22441 ggtgccgtct cattaccgtg gatccccgca gaaatcacca gcgcgccttg cggtggcgtt
    22501 aatggcgtca gttccagcac accatcgccc agccagcgcc agcgaacgcc gttgatttct
    22561 cgctcggtga taaccggttt tttacccgtt aaggtcagag caagaaaatt atccattgcc
    22621 ccctccctcg cgctggaacg gataaaccga accgagattc agtaattggc tcaatacatc
    22681 cagtgcttcc cgcccttcgc gcagcaattg cgggtcggcc agatcggcag cagtaagacg
    22741 atcgcggtag taacgatcca cccagtcatt gagcacatta aacaaggtat cgttcatcat
    22801 caccgccgga ttcaccgccc ggcgttcttc ttctgtcaat accccccgca accgcaggca
    22861 cgccgggccg ccgccattcg ccatgctttc acgtaagtca aagactttta gttcactaat
    22921 cgggttgtca gcggcaagga gttcattgag ataaccccat actccggcgt gctcccgaca
    22981 ctcctgaggc agcaccaaca tcatggaacc atcatcgcgg ctcaacagtt ggctgttaaa
    23041 cagataggta gacaccgcat ccgacacgga aacctgagtt gccggaactt ctatcgccat
    23101 aaaaccgttc acccgcgtgc cagtaactgt gactggcgag cgaacgcctg ttggtggcaa
    23161 aacagcacct ggcggttact cacggcaatc acgtcattat gaaaaacgct ctggtcgata
    23221 acgtccgggt tttgctgggc gaaaatcacc tgttggggat tcacctgatt aagccttgcc
    23281 accgcctcgc tggcttcgcg agtctgtcgc gccggataac gggaaggccg ggtatcattg
    23341 ccttcttctc gcccgtagac aaaaagtagc atacccggtt caccgtaatg accgccgaga
    23401 cgattgtggt ttgccgcccc ctcatcaccg agcaacgcta cctgcggcaa cgccgaatgg
    23461 acgctaaatt tctcttcgtc cttaaaaatc gcttttaaca gcgattcagt gacgggcgct
    23521 tccagcgaac ggtgaaattt attgttcagg ttggcaacgg tgagatgcac tttgccatcc
    23581 agcgtatcgg cagatggcgc gatcgttgcc gcattggcta cccacattgg cgaagcggaa
    23641 ctgacgctgg aaagccagtg cggtgcctgg cgtgcaactt tttccagtac ctgctcatcg
    23701 ctaccactga atcccaactg acgcagcacc ggaataaacg gacgctcgtg cggcgggatc
    23761 acggcctggg ggaatcccgc atcggcaagg gctttcattt tcagtaagcc ctgttttgcc
    23821 gccagtcgcg ggttagacac ctgaaaacgg tgacgggtag aggcttcatt accaaacgac
    23881 aggcccgcgt aatgatgcgt cagccctacc agcccgtcga aattaacttc ccaggcgttc
    23941 atcgcaccac ctcatcggaa aaatccagcc cggggttaag cgtggcgggc aatgttaacg
    24001 agtccgactc caggctcgcc ataggccatg cgcagtaatc tgcggcatac caggcgctgg
    24061 ggcgatggtt accggaagca ccaatgccgc cgaatggggc ggtactggca gcaccagtaa
    24121 gcggtttgtt ccagttaaca atccccgccc gcgcctccag caacagttga tcgaattttt
    24181 cccgctcggg ggaaaccaga ccgcaaaaga gtccgaagcg agtgttgttc gccatccgaa
    24241 tcgcctcatc aaaagtatca taacgccaga cgcgcaaaag cggcccgaac acctcttcat
    24301 ccggcacacc gccaacgccc gtcatttcaa tgatccctgg tgtcagcaac gatgtccctg
    24361 cttgtaataa gcgcggcgca agcagggttc gtccgcccat cgcttccagt tgctgccagg
    24421 cagtaaccac ctgctgtgcg gcctgttcag aaatcagccc gccaataaac ggctgcggtt
    24481 cgtcatccca gttgcccggc gttaatcgct ggctgacggc aaccagacta gcaagaaacg
    24541 catcgccctg cgctccgctt ttgagcaata aacggcgggc gcaggtgcag cgttgcccga
    24601 ctgtgacaaa cgccgactga atggtcagat ggacagccgc gtcgatatcc gccacctcat
    24661 cgataattag cggattatta ccgcccatct caagggcgag aattttctcc ggctgaccgg
    24721 agagctggcg atgcagctgg tagccggtat tggcgctacc ggtaaacagc aaaccgtcga
    24781 gatcctccag cgcactcagc gcctgacccg tttcacgccc gccctgcacc aggttcagca
    24841 cgcccggcgg caagccagcc tgctgccata aacgcattac cgcttcgcca ctccacggtg
    24901 tcagttcgct gggtttaaag ataatggtgt tacctgccag caatgccgga acgatatgtc
    24961 cgttcggcaa atgaccaggg aaattatacg gcccaaacac cgccagcacg ccgtgcgggc
    25021 gatgtcgcag gctcgccgca ccgtccggca tttcactacg ctgctcgccg gtacgaacgt
    25081 gatacgcctt aattgatatc gcgattttat tgatcatcgc cgtcacttcg gttgccgctt
    25141 cccagcgcgg cttacccgtt tctctggcaa taatcgcggt taattcggct ttattgcttt
    25201 ccagcaatcc ggcaaagcgt tcgacaacga cctgacgttc agccaatgag agccgcgccc
    25261 agcgcggaaa cgccgcacgg gctgcccgac aagcctgttc gacctgagcg gcatcggcat
    25321 cattgccttg ccataacacc tcgcccgata ccggattacg cttcacacgc gatgcgccct
    25381 ggcccgttat ccagtcaccg ttaatccata aagtcatgct gttttctcct ctgcgcacag
    25441 gcgcaccaga cgaacgcgat cccctgcgtg gcatttgagg gcatccagtt gtgcggcggt
    25501 taaaatcaaa cgctcggttg ccggatcggt acgcgccagc accacgcgga aatggtgata
    25561 attttcattg gcgaccaggc aggccggaaa atcgccctgc gcaggctgcc cttctgccac
    25621 ttccaccagc cgacttttac ggatggcgcg cacgcggtcg atgtcacact caagcgttgg
    25681 cccgccgtcg aagatgtcga tatagttgcg gtaacgaaaa ccttctttct ccagcaccgc
    25741 gcgggcaggc gcggtttgcg gatgtacctg accgatgacg tcctgggctt cctgggataa
    25801 aaagtgggta tagatcggat gtttcggcat cagttctgca ataaatgcct tttgcccggt
    25861 gccgcagaga aaatcggcgc ggctaaaatc catcgaaaag aagcgtttac cgaggctttg
    25921 ccagaacggt gaatagccgt gttcgtcaat caccccgcgc atttcagcaa ccactttgtc
    25981 attaaacttg tcgcgaaaag ccgccataaa cataaagcgc gatttcgaca gcaaatagcc
    26041 gttgccctct ttgcgccagt ccgggtcgag aaacagcgtg cacagctcgc tgctgccggt
    26101 gtgatcgtta ctaagaaaca gcgtcggcaa tgcgttatag acattcagct cttttgaggc
    26161 gtgaaccaac gtgccgacgc gatagttgta ccagggatcg ttcagcccaa ccgccacctc
    26221 aatagcgcaa atccccgcca cggtgcctgt ctcgctatct tccagcacga acacatagcc
    26281 ctgctcgctt ttgggcagtt caccttgcca ggttttgatt gccctttcga tacgcgccga
    26341 aagcgtggct tcattggcgg gaagcgacgt caggccgccg cccgttttgc tggcaagctg
    26401 catcagcgcc gagacatctg agcgctcaac gggacggatg accatcatga tgaacctcgg
    26461 ctaacaaagt gttcgcaagc agctgcaaag cgatccagtc cggtcgtcac ctcttcttcg
    26521 ctgacattga gcgcaggcgc aaaacgcacc acgttgccac ccgcaatcag taccatcacg
    26581 cctgctttcg ccgcttccag agagatctgt ttcgcttgcc cggcgtaatc ggcattcagt
    26641 acacagccaa tcaacaaacc taagccgcga acttcactga acaaaccata gcggtgatta
    26701 atagtattaa gacgctcaac aaaccagtcg tgacgctgtt taacgccatt aagcatctct
    26761 ggtgtgttga tgagctccag cactttgcct gctaccgccg aggccagcgg attaccgcca
    26821 taggtggtgc catgagtgcc aacggtcatc acgcgggcgc actcttcggt tgccaacaac
    26881 gcaccgaccg ggaaaccgcc gcccagcgct ttggcggtgg ttaacagatc gggcgtcacg
    26941 ccgtagtgca tataggcata cagctcacca gtgcgcccga cgccggtttg tacctcatca
    27001 aaaatcagca aggcattgtg gcggtcacac aattcacgca gaccttgtaa aaacgcgttg
    27061 ctggctggca ccacaccgcc ttcgccctag atgggctcga caatcaccgc acaggtggcg
    27121 tcgtcaatca gcgcgctggc agagttaata tcgttatatg cagcatgacg aatatccgcc
    27181 ggcagtggcg caaaatcctg tgaataggct ggctgcccac ccgcactgac agtaaacagc
    27241 gtgcgaccat gaaacgcatt tttgaacgcc acgatgccac tcttatggct gccgtagcgg
    27301 tcgtgagcga atttacgcgc cagttttagc gccgcttcgt tggcttccgc accggagtta
    27361 caaaagaaga cgcgatcggc aaacgtggcg tcgatcaatt ttttcgccag tcgcagtacc
    27421 ggctcgttgg tgtaaccgtt gccggtatgc cagaacttac tcgcctgttc gttcagcgct
    27481 tcacgcagtt ccggatgcgc atggcccagc gcgttcaccg caatgccacc cgcgaagtcg
    27541 atatactctt tcccctgctg atcccacaag cgcgaacctt cgccacgtac cggtataaag
    27601 ggtgccggag cgtaaacagg tatcatccat tcatcaaagt tttcacgcgt aattggctga
    27661 gacatagcga cctctacagt aaataattcg ttatttgcat gttaacaata agtaatgttt
    27721 gcgctgtaaa tgtagattgc agggttcgtg ccagccagtg ataaaagtgc ataaacggcg
    27781 gaggctaact ggaaatcaag gagttataac caaaccatat gcatttaaag tgcatataaa
    27841 gtgaatacgt ttgcgatgtg ggtgaataaa aagaataaaa aacgcaatgt tatgcagaag
    27901 taaaatataa ttctagaatt gtgatcattg acgaaattta ctggaaatta ctgcgccatt
    27961 ctgacgcagc gcgcaccaaa agcgggcatt ttttgcgcca tcattgacat cattaacaac
    28021 catcgatcaa atcacttaac aacaggcggt aagcaacgcg aaattctgct accatccacg
    28081 cactctttat ctgaataaat ggcagcgact atgaaatttg tctcttttaa tatcaacggc
    28141 ctgcgcgcca gacctcacca gcttgaagcc atcgtcgaaa agcaccaacc ggatgtgatt
    28201 ggcctgcagg agacaaaagt tcatgacgat atgtttccgc tcgaagaggt ggcgaagctc
    28261 ggctacaacg tgttttatca cgggcagaaa ggccattatg gcgtggcgct gctgaccaaa
    28321 gagacgccga ttgccgtgcg tcgcggcttt cccggtgacg acgaagaggc gcagcggcgg
    28381 attattatgg cggaaatccc ctcactgctg ggtaatgtca ccgtgatcaa cggttacttc
    28441 ccgcagggtg aaagccgcga ccatccgata aaattcccgg caaaagcgca gttttatcag
    28501 aatctgcaaa actacctgga aaccgaactc aaacgtgata atccggtact gattatgggc
    28561 gatatgaata tcagccctac agatctggat atcggcattg gcgaagaaaa ccgtaagcgc
    28621 tggctgcgta ccggtaaatg ctctttcctg ccggaagagc gcgaatggat ggacaggctg
    28681 atgagctggg gggtggtcga taccttccgc catgctaatc cgcaaacagc agatcgtttc
    28741 tcatggtttg attaccgctc aaaaggtttt gacgataacc gtggtctgcg catcgacctg
    28801 ctgctcgcca gccaaccgct ggcagaatgt tgcgtagaaa ccggcatcga ctatgaaatt
    28861 cgcagcatgg aaaaaccgtc cgatcacgcc cccgtctggg cgaccttccg ccgctaattt
    28921 agcagctctc ctggctcaaa ctgggtcagg agaattaacc ttgagaaaaa tcaacaaact
    28981 gtcagtaatg atttgttgcc tgccgtcctt tgttataccg tcactgcgtt tttagttgtc
    29041 tgaccacttc tctattatca agtttgatat aggaaactcc acgatgaacg ctgagcgtaa
    29101 ctttctgttt gcctgtctta tttttgcgct ggtcatttac gctatccacg ctttcggttt
    29161 attcgatctg ctcaccgatt taccccactt acagacactc attcgccaga gcggattgtt
    29221 cggttatagc ctctatattc tgttattcat caatgccccc tctttctgtt accaggaagc
    29281 atactggtga tcgctggagg aatagttttt ggcccgctct taggacacaa ctctcattaa
    29341 ttgccgcaac gctggcctcc tcgtgctcat tcctgctggc gcgctggctg ggacgtgatt
    29401 tactgctgaa atacgtgggc catagccata ccttccaggc cattgaaaaa ggcattgcgc
    29461 gtaacggtat tgattttctt attctgaccc gcttaatccc gttgtttcct tacaatattc
    29521 aaaattacgc ttacggattc accaccatcg ccttctggcc ttataccctt atttcggcac
    29581 tcacgaccct gcccggtatt gttatttata ccgtgatggc aagcgatctc gccaatgaag
    29641 gcattacgct gcgctttatt ttacaactct gtctggcggg cctggcgctg tttattctca
    29701 tccagctcgc aaaactctac gcccgacaca aacatgtgga tctgtctgct tcgcgccgca
    29761 gcccacttac tcacccaaaa aatgaaggat agaacgatgt cgcaacatta ttcagtgtca
    29821 tggaaaaaag gactggctgc actctgctta ctggctgttg ccgggcttag cggctgcgat
    29881 caaaaagaga atgcggcggc aaaagtggaa tacgacggac tttcgaacag ccaaccgctg
    29941 cgcgtcgatg ccaataacca tacggtaacc atgctggtgc aaattaatgg tcgtttcctc
    30001 accgacgaca ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg
    30061 tttatggctt atgcgccccc gaaagcattt tatgaagccc tgaaagaggc aggtggtacg
    30121 ccgggcgaaa acatgacgat ggataataaa gaaacgactc atgtcacagg cagcaaactg
    30181 gatatttcgg tcaactggca aggggcggca aaagcgtatt ccttcgatga agtgattgtt
    30241 gacagtaatg gcaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag
    30301 aagaaaacgg gttgcctggt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca
    30361 acatacactt atggtgcggt ggaaaaacac ggcgaagtga aattcaaagg caatgcctca
    30421 gttctcccgg cggataacac gctggcaacg gttaccttta aaatcaccga ataaagccaa
    30481 gataaaggat gatgaagata caatcgcgta aaatctggta ctaccgtata accctcatca
    30541 tcctgttatt cgctatgctg ctcgcatggg cgctgcttcc cggcgtccat gagtttatca
    30601 atcgcagcgt tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt
    30661 cttacggcgc actggcggca gttgtctcgt tcttgttgat gattttacag gccattgctg
    30721 caccgctacc tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg
    30781 gcggcttgct gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca
    30841 gagtgatggg ccgcgaagtg gtggaaaaat taaccggcaa aaccgtgctt gacagtatgg
    30901 acggcttttt cactcgctac ggcaaacaca ccatcctggt ctgtcggtta ttgccttttg
    30961 tccctttcga tccaatcagc tatgctgccg gtctgacttc aatacgtttt cgctcgtttt
    31021 ttatcgccac cgggcttggt caattaccgg cgactattgt ttattcctgg gcaggcagca
    31081 tgttaacagg cggtactttc tggtttgtca ccgaactgtt tattctgttt gccctgaccg
    31141 tggtgatttt tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gactgatggg
    31201 tttaccgccg cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg
    31261 ccattacggc gaggtgctaa gcccgattcg ctggtggggg cggatcccgt ttatctttta
    31321 tttggtgtca atgtttgttg ggtggctgga gcgcaaacgc tcaccgctcg atccggtagt
    31381 gcgatcgctt gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gcgtggatat
    31441 caccagtatg aaagtcgccg agcgtaccgg cagcaccgat aaactgctgg cagtggctga
    31501 ctggcggcaa agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc
    31561 cgcaagcgtc acaccgccaa cagtcgatga tgccctgcgt acccgactgg ctgcgcattt
    31621 tgacgctcag gcgctcaccg aactgacggc attgatcggc ctgcaaaacc tgtcggctcg
    31681 ttttaattct gccatggaca ttcccgctca ggggctgtgc cgtattccgg aaaaacgttc
    31741 ttaaggaggg aagatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttcttt
    31801 ggcaacacat gccagtgact ggcaggaaat taaaaatgag gccaaagggc aaaccgtctg
    31861 gtttaacgcc tggggcggcg ataccgcaat taaccgctat ctcgactggg ttagcggcga
    31921 gatgaaaacc cattacgcta taaacctgaa gattgttcgt ctcgcggatg ccgcagacgc
    31981 ggtgaagcgc attcagaccg aagctgctgc cggtcgtaaa acgggcggct cggtggatct
    32041 gctctgggtg aacggcgaaa acttcc
//
LOCUS       DAJVRP010000042        31599 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVRP010000042 DAJVRP010000000
VERSION     DAJVRP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.042"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..606)
                     /gene="capU"
                     /locus_tag="OQN12_001966"
     CDS             complement(<1..606)
                     /gene="capU"
                     /locus_tag="OQN12_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1654299.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
     gene            complement(609..1451)
                     /locus_tag="OQN12_001967"
     CDS             complement(609..1451)
                     /locus_tag="OQN12_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1654300.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1637..2354
                     /locus_tag="OQN12_001968"
                     /pseudo
     CDS             1637..2354
                     /locus_tag="OQN12_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3027..4535
                     /gene="ltrA"
                     /locus_tag="OQN12_001969"
     CDS             3027..4535
                     /gene="ltrA"
                     /locus_tag="OQN12_001969"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1654301.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPEPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            4616..5103
                     /locus_tag="OQN12_001970"
                     /pseudo
     CDS             4616..5103
                     /locus_tag="OQN12_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5186..5443
                     /locus_tag="OQN12_001971"
     CDS             5186..5443
                     /locus_tag="OQN12_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654302.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(6001..6768)
                     /gene="fecE"
                     /locus_tag="OQN12_001972"
     CDS             complement(6001..6768)
                     /gene="fecE"
                     /locus_tag="OQN12_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCS1654303.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(6769..7725)
                     /gene="fecD"
                     /locus_tag="OQN12_001973"
     CDS             complement(6769..7725)
                     /gene="fecD"
                     /locus_tag="OQN12_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCS1654304.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(7722..8720)
                     /gene="fecC"
                     /locus_tag="OQN12_001974"
     CDS             complement(7722..8720)
                     /gene="fecC"
                     /locus_tag="OQN12_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCS1654305.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(8717..9619)
                     /gene="fecB"
                     /locus_tag="OQN12_001975"
     CDS             complement(8717..9619)
                     /gene="fecB"
                     /locus_tag="OQN12_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCS1654306.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(9664..11988)
                     /gene="fecA"
                     /locus_tag="OQN12_001976"
     CDS             complement(9664..11988)
                     /gene="fecA"
                     /locus_tag="OQN12_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCS1654307.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(12075..13028)
                     /gene="fecR"
                     /locus_tag="OQN12_001977"
     CDS             complement(12075..13028)
                     /gene="fecR"
                     /locus_tag="OQN12_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCS1654308.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(13025..13546)
                     /gene="fecI"
                     /locus_tag="OQN12_001978"
     CDS             complement(13025..13546)
                     /gene="fecI"
                     /locus_tag="OQN12_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCS1654309.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            13844..14026
                     /locus_tag="OQN12_001979"
                     /pseudo
     CDS             13844..14026
                     /locus_tag="OQN12_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14020..14124
                     /locus_tag="OQN12_001980"
                     /pseudo
     CDS             14020..14124
                     /locus_tag="OQN12_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14464..14721
                     /locus_tag="OQN12_001981"
     CDS             14464..14721
                     /locus_tag="OQN12_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCS1654310.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(15041..15172)
                     /locus_tag="OQN12_001982"
                     /pseudo
     CDS             complement(15041..15172)
                     /locus_tag="OQN12_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            15454..16812
                     /locus_tag="OQN12_001983"
     CDS             15454..16812
                     /locus_tag="OQN12_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCS1654311.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(17051..18436)
                     /locus_tag="OQN12_001984"
     CDS             complement(17051..18436)
                     /locus_tag="OQN12_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1654312.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(18486..18833)
                     /gene="tnpB"
                     /locus_tag="OQN12_001985"
     CDS             complement(18486..18833)
                     /gene="tnpB"
                     /locus_tag="OQN12_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1654313.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(18830..19210)
                     /locus_tag="OQN12_001986"
     CDS             complement(18830..19210)
                     /locus_tag="OQN12_001986"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCS1654314.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(19565..19999)
                     /locus_tag="OQN12_001987"
     CDS             complement(19565..19999)
                     /locus_tag="OQN12_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCS1654315.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(19987..20388)
                     /locus_tag="OQN12_001988"
     CDS             complement(19987..20388)
                     /locus_tag="OQN12_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654316.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(20648..21217)
                     /locus_tag="OQN12_001989"
     CDS             complement(20648..21217)
                     /locus_tag="OQN12_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCS1654317.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(21962..22105)
                     /locus_tag="OQN12_001990"
                     /pseudo
     CDS             complement(21962..22105)
                     /locus_tag="OQN12_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(22439..22705)
                     /locus_tag="OQN12_001991"
     CDS             complement(22439..22705)
                     /locus_tag="OQN12_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654318.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            22846..23052
                     /locus_tag="OQN12_001992"
     CDS             22846..23052
                     /locus_tag="OQN12_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1654319.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            23147..23749
                     /locus_tag="OQN12_001993"
     CDS             23147..23749
                     /locus_tag="OQN12_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654320.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            23920..24117
                     /locus_tag="OQN12_001994"
     CDS             23920..24117
                     /locus_tag="OQN12_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654321.1"
                     /translation="MFFTARYTPGSQQTECAGAVIHQKWHAAVHTHPGDNMYVATAIP
                     ALWKRLTQPAGYSFRHPGMSA"
     gene            24949..25866
                     /locus_tag="OQN12_001995"
     CDS             24949..25866
                     /locus_tag="OQN12_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654322.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            25951..26823
                     /locus_tag="OQN12_001996"
     CDS             25951..26823
                     /locus_tag="OQN12_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCS1654323.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            27041..29425
                     /locus_tag="OQN12_001997"
     CDS             27041..29425
                     /locus_tag="OQN12_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCS1654324.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            29422..30327
                     /locus_tag="OQN12_001998"
     CDS             29422..30327
                     /locus_tag="OQN12_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCS1654325.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            30324..31394
                     /locus_tag="OQN12_001999"
     CDS             30324..31394
                     /locus_tag="OQN12_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCS1654326.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT         7739 a         8122 c         8349 g         7389 t
ORIGIN      
        1 aaattcgtgc cctttttccg ggcgcaacat tcctacctga gcaattacgg ggcatcctct
       61 tcgggacgcc agccaggaaa gaacactcgg aggcaaactg tttcgggaat caacgtataa
      121 ttcctggaaa tcaaagccag gaggcacaac agtaacccga gttcgacatc cttcctgctc
      181 cagatgtgtc ttcatatttg ttccgggaac aatcacctca tcgcaaaaat gatttattga
      241 aaaaaatttt gtttttcgtg tcaaatatgt cttttgcctg ataattctga atggatgttt
      301 ccaggtaaaa aaccgtacca aaccaacaat attactatca tgtccactgt gacaaacgat
      361 tgcatcgggc tgaaaactat gaactattcc gagtaatctc catacagtag gaatgtgaag
      421 actgtttcga aataacgcga atgtgatatc aattccaaac ttactggctt caaaagcaat
      481 tttgctgttc tccctgcaga caagcagaac tgaatgcccc attttcttca gggccttcat
      541 ttgagcaaca gcctgtaatt cctgaccacc tatattcggt gatgattccg taaatagtat
      601 attcattatt agtcatccgg taatcttggc cccttatgaa gggccagcaa cgatgaaaaa
      661 aacggtgttg tataataaaa aagtcgtctt ttcagccata cgcaattttc ccgttccttg
      721 gtgcttattc ttcctatccg tgtagttcct ttagcaggag cattcattct tctttctgtt
      781 gtatataaat aatggaatcc aagttcttca gcgatctgaa tataatcctt gttataataa
      841 ccttccggcc aacacaaatg tttactgcat tttcctgtca ttttttttaa atattctctc
      901 ccgctcaata agtcctgccg aagatgttta cactgttctt ctcgtgaagt gaacttttta
      961 tcccaacgtg tatgagtatg tgtatgaaca tgaaattcga ccagaccaga ttgcaacatt
     1021 tcgttcactt cagaccagcg aagcataaca ttatcagcat tcccggttgc tatttgatgt
     1081 tcgcaatcgc gatgagaata ttcctttcca ggagagtgac gaaccggacc atttccaata
     1141 aaactggtaa ttaaaaaaat atgtgccttg agattaaact cgtttaataa aggatatacc
     1201 tgaaaccagt tgtcaaggta tccatcatca aatgtcaaca taacgctttt acggggaagt
     1261 tttccgcccc gataaaaaaa ctccagttca tctgatgaca gtgttttcca gttgttttca
     1321 gccaaccatt tcatttgttt ccggaatgtc tcgggagaaa gtgtaaccag tcctgggcaa
     1381 tggctgacat gatgatacat taataccgga acatgatttg ctttaaacaa aatgccccct
     1441 ccgtttaaaa tttaaagttt ctggcaccca gtaagtagag ataaaaggaa agcctaataa
     1501 aggtggtgaa ctttatatac tactaaatca aacatcaaca atttataaac aataatatat
     1561 tatcatatct tttaaaaacg gctcactaag aaccttgagg tggcacacta aatttagcta
     1621 cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgccataatg aaaaaagaaa
     1681 tttcagcgca gagtttaaac gcgaatccgc tcaactggtc gttgaccaga aatacaccgt
     1741 ggcagatgca gccagcgcta tggatgtcgg cctttccaca atgacgcgat gggtgaaaca
     1801 attacgtgat gagcggcagg gaaaaacacc aaaagcctcc cccattaccc cggaacaaat
     1861 tgaaatccgt gagctcagga aaaagctaca acgtattgaa atggaaaatg aaatattaaa
     1921 aaggctaccg cgctcttgat gtcagactcc ctgaacagtt ttcgataatc gggaaactca
     1981 gggcgcgtta tcctgtggcc actctctgcc atgtgttcgg ggtccatcgc agcagctaca
     2041 aatactggaa aaaccgtcct gaaaaaccag acggcagacg ggctgtatta cgcagccagg
     2101 tacttgaact gcatggcatc agccacggct ctgccggagc aagaagcatc gccacaatgg
     2161 caacccagag aggctaccag atggggcgct ggcttgctgg cagactcatg aaagagctgg
     2221 ggctggtcag ttgccagcag ccgactcacc ggtataagcg tggcggtcat gagcacgttg
     2281 ctatcccgaa tcatcttgag cgacagttcg ccgtaacgga accaaatcag gtgtggtgcg
     2341 gtgatgtgac ctatagtgtg cccggagttc agggcgggca tggatgctta aatgaaccgc
     2401 gagtctgtct ggaatattga accggtaact cacgatgaga aacccaacaa tcccaccggg
     2461 tgtgacggtg gagaacctga gcggcagtga cctgcggcat gcccgcaggg tgatgtaacc
     2521 cgctgacaac ggggattgag gcgagatcac taagccgaga tgatcctcaa ggttaagtac
     2581 tgaaaggctg aagaacatga acccgttaat ccgcctctgt gggttgaaaa cgtcaccacg
     2641 gcctacgtga tctgacaggc cgtgcaggag gaactggcag tgatacgtaa gcactgccgg
     2701 tcgaaggtgt tttgacatgt atgcgaaaca ccggggcagc agcgtctatc acgcttgcgt
     2761 tgctgacttc tgccaacttg cggcaagcaa ggataaagag tgcgacgggc agcctcctca
     2821 gtatgcctga gtccaggcag gtaaaccggg gaaggtcagc gacggatgtt aagggggcat
     2881 ggctccgatg acgcgctgac tggcggagct tccgtagtag tccgcgatgg ggaaagccca
     2941 ttacatggcg aagggaagca gtttgaatgt gtttgcgacg tgaattaact ggcctaacga
     3001 ggtgaagacc tttgataatc agcgaaatgc aacgcaagct tgccacatgg gcagccaccg
     3061 atccgtccct acggattcaa cggctgctgc gtctgataac acaaccagaa tggctggctg
     3121 aagcggcgcg gatcacgctt tcatcaaagg gggcgcatac ccccggcgtt gatggcgtga
     3181 acaaaacaat gctacaggcc agactggctg ttgagctgca aatcctcagg gatgaattac
     3241 tctcaggcca ctaccagccc ttgcccgcca gacgggttta catccctaaa agcaacggca
     3301 aactgcgacc actgggtatc cccgcgttgc gggatcgtat tgttcagcgg gccatgctga
     3361 tggcgatgga gccgatatgg gagagtgatt ttcatacgct ctcatatggc ttccggcctg
     3421 agcgcagtgt ccaccacgcg atccgcacgg tgaaattaca gctcacagac tgcggtgaaa
     3481 cccggggacg ctgggtgatt gaaggcgacc tgtccagtta cttcgacacc gtacatcatc
     3541 gactgctgat gaaggctgta cgccgcagga tcagtgacgc acgtttcatg actctgctgt
     3601 ggaaaaccat caaggcggga catatcgatg tcggtctctt tcgggcggcc agtgaaggtg
     3661 tgccacaggg cggtgttata tcgccgctat tatcgaacat catgctgaat gagttcgatc
     3721 aatacctgca tgagcgctac ctgagcggga aagccagaaa agatcggtgg tactggaata
     3781 acagtatcca acggggccga agtacggcgg tcagagaaaa ctggcagtgg aaacccgcgg
     3841 tggcgtactg ccgctatgcc gatgattttg tcctcatcgt caaaggcacc aaagcacagg
     3901 cggaagccat cagggaggag tgtcggggtg tgctcgaagg cagtctgaaa ctcaggctga
     3961 acatggataa gactaaaatc acccatgtta atgacggctt tatctttctg gggcacagga
     4021 tcattcgcaa acgcagtcgt tatggcgaga tgcgagtggt ctcaacgatc ccgcaggaga
     4081 aagccagaaa cttcgccgca tcgctgacag cactgttatc aggcaactac agtgaaagca
     4141 aagtcgatat ggctgaacaa ctcaaccgaa aactgaaagg ctgggccatg ttctatcagt
     4201 tcgttgattt taaggccaaa gtcttcagtt atatcgaccg tgtcgtgttc tggaagctgg
     4261 ctcactggct ggcccgcaaa taccgtacag gtatcgcttc cctgatgagg tggtggtgta
     4321 aatcaccgga accgggtcag agcaaaacgt gggttttatt tggtaaaacc aatcacggca
     4381 agctcagcgg cgaaatactg taccggttgg tggggcaagg caagaagctg ttccgctggc
     4441 ggctacccga aggtaatccc tatctgagga cggagaccag aaacacgtat acatcgcgct
     4501 ttacagaagt ggcaatggcg ttcgccagca tttaaatgga gagccggatg cgctgaaagg
     4561 tgcacgtccg gttcggggag gagaggcagg gaaatagtcc gactacgccc tgcctcttac
     4621 tctactctgg acgggtaagc gctgggcgta cctcgccgtt gttctcgacc tgttcgcaag
     4681 aaaaccagtg ggctgggcca tctcgttctc gccggacagc aggcttacca tgaaagcact
     4741 ggaaatggca tgggaaaccc gtggtaagcc cgtcggggtg atgttccaag cgatcaaggc
     4801 agtcattata cgagcaggca gttccggcag ttactgtggc gataccagat cagacagagt
     4861 atgagccggc gtggaaactg ctgggataac agcccaatgg agcgcttctt caggagtctg
     4921 aagaactaat gggtgccagc gacgggctat gtaagcttca gcgatgcagc tcacgcaata
     4981 acggactata tcgttggata ttacagcgca ctaagaccgc acgaatataa tggtgggtta
     5041 ccaccaaacg aatcagaaaa ccgatactgg aaaaactcta acgcggaggc cagttttagt
     5101 tgaccacaac agactacctg aagggagccg cggtcgccta gcagttgcag tagcaggaga
     5161 tcatccagcc gcagtacagg tcacgatgac tctggttaat gataccggct ttgaccccgt
     5221 attttccggc tctatcgctg aatcatggcg tcagcagccg tgcacaccat cctattgttg
     5281 tgactgggag gctgccacca tgcttcgcgc tttccctctg gcgaaaaagg gagaaggacg
     5341 ggcccgtctg ccttcacttt atgccagctt cggtaagctg ggtgagacac cgactcatga
     5401 agatatcatt gataacaatc gatccatcaa ctggcctgta taacgtggct gccggtgatt
     5461 aagaaagctg cacctaccta agtagtagca aacgcacact ttttagaaaa atcgatggtc
     5521 agaaactgga ttagcaattc cgttccaggg ttgcttttga tttacgttgg cgtctgatca
     5581 ttgatttatc ctcaaaagcc caacctcatt ggtaatgaac cagctccgtg aatgtccgct
     5641 ctggcacaga gcgaaatttt ttgatctccc cccctgaaat ctaaacttag tcatgtcacg
     5701 tttttgggtt tctaaaattt taacttcgcg tttttcgttg ccgtaagggt tatacagaaa
     5761 tgtccgttaa gcagagttca aaattgattg ccgtgatcac gactggtttg aaagccgcgc
     5821 ccaagcctgt acagctctgg tttgcgttga ttatgaacct gtcagcctaa agcaagcgga
     5881 tggacgatga gtattggtaa tctttcagag tccggaaaag ttcagcccca gtctgaacag
     5941 gcttgctggc gccagtccag tttcattcag tcgtggtttg gttcttacgg cctgtgcaat
     6001 ctacctcatt aggcacatcg gcctgccaga taccggctcg gggtgtattt ccgcttccac
     6061 gctgaatact gttctcagca atcctggggt catcacctct tctggtgtgc cttgcgccat
     6121 aacatgtccg tttgccatta ccaccagttg atcgcagtac cggctagcct gattaaggtc
     6181 gtgcagcaca gcgaccaccg ttttcccctg agtccggagt tcgcccatca accgcatcag
     6241 gtccacctgg tgattgatat caagataggt ggttggctca tcaagtaata caacgggcgt
     6301 attctgggcc aggaccatcg ccagaaatgc gcgctggcgc tgaccgccgg aaagctcggt
     6361 taaccgacga acggcaagat gattgatccg ggtctggttc atggcgacat taactcgtgc
     6421 attgtcttca gcggagagac gcccccagag tgacagccag ggattacgac catacgaaac
     6481 cagctcctgg actgtgatcc cctctggcgt taaatggtgc tgaggcagca gcgaaagcct
     6541 gcgggccaac tggcgtgatg agagcatatt tatgggatta tcgccgagaa atacggtgcc
     6601 agactgcggc attaaaagcc gcgaaaaaca gtttaacagc gtcgatttcc cgcaaccgtt
     6661 agggccgatc agggcggtga tcttccccgt tggcagtgag agtgaaacgt cgttaagtac
     6721 cttgtctgtc ccgtaactga ccgtcagatt ttcagttcgt aaagtcattt atcgcattct
     6781 cacaagcaac cagacaaacc acggcgcacc gataatggcg gtcagcacgc caaccgggag
     6841 ctccagtggg ggatgaataa ttctcgccag cagatcggca accaccaaca gcaacgcacc
     6901 tgtcagggct gaaacaggca gcagtctgcg gtgacgtcca ccggtgatgc tacgcatcat
     6961 atgcggcacc acgagaccaa taaagctaat cgggccgcag gcggccacgc cggtagatgt
     7021 catggcgaca gctagtaaca aagcccagaa tcgggtatgg ggcaccgaca caccgagcgt
     7081 ggtggcgcgc gcatcgccga gtgcaaggag gtcgagatcg cggcaaaaac tcaggctcag
     7141 cggcagaaat aaaatcatca gcgggatggc aatcttcaca aagctccagt cacggcccca
     7201 taagctgccg gtcagccaca gcagggcgtt gttcacatcc tgcgggcgcg agagcatcag
     7261 ataatccgtc aggctggccc agcatgcaga aagcgccacg ccggtgagcg ccagcttcat
     7321 cggctggtgg gtctttgcca gcatcttcag taatatcaac cccgccatgc cgcccgcaaa
     7381 ggccagcagc ggcagcacca tcacgggcag tgacggcata agaagtagag cccccacaga
     7441 ggccaggctg gcggcatggt taacgccgag aatatccggt gatgccagag ggttgcgcac
     7501 aatcccctgt atcagcacgc ccgccacggc gagggctgca ccgacaaaca gtgccagcag
     7561 caagcgcggc agtcggtact ccatcaatac ataataatgc tcgcgtccgg cctgccagtc
     7621 ggtcagcagc gcgcgccacg gcacggggat cactcccata tggagtgata acagcgcaca
     7681 gcccgccagg gcaagggtga tgaaaataac cagcgcaatt ttcatcctcg cctcctcaca
     7741 agccagacaa agcaagggct gccaatcagc gccagcactg cgcctgcggg cagatctccg
     7801 gggaaggcca gcgcgcgtgc gagtacatct gccagcagca tcagcgtggc ccccagcagc
     7861 atgctcactg gcagtacgtt gcgctgatcg aagcctgccc agaagcgcgc cagatgtggc
     7921 accagcagac cgataaacgc caccggacct gccacgctga cgcacgcacc aaccagaagc
     7981 agcactaaca tattgatgac caaacgtagc ctcgtcaggt tcactcccag cgtatgggcg
     8041 gtgctgtcgc tgaggttgag caggttcagt tgattcgcca gcagtaacac gacagggact
     8101 gcagtgacca ccaccggcaa gagctgccag acatcctgcc agcgggcgtg ggacactccg
     8161 cctgccagcc agtaaaagat gccgtaagca tgatcttcgg ccagcagcag ggtgatgcgg
     8221 gtaaggccca tacaaaaggc cgacagcgcg atacccgcga ggatcagttt gtttctgtca
     8281 tgggtatgac gaaatccgcc tcctgcggtc atgaccagca gccagctcac gccgcccccg
     8341 catgccgcga tgaacgacag agaatagcct gcaatcggcg tcggactcag cgcgctggta
     8401 agcgccatag ccagcgccgc gccgctgtta atgccgagca gtgaaggaga ggccattggg
     8461 ttgtgggtca gggtttgcag cagcgtgccc gcgagcgcca ggcttgcgcc gatcagaacg
     8521 gcgaccaggc ttcgtggcaa acgaaggttt tgcaccagcg cttctggtag cgttggcgtg
     8581 tgtccaggca gcagggcgcg ggttgcatct gctccggaaa caggaatggc cgagtagcaa
     8641 aacagactca gccagaaaat aataataagt gctgcaacgg gaagccccca cagcagcatc
     8701 gggtgtttta tcgcggtcat ttcacaacgg taagcggctg atggtggaag atttttaccg
     8761 tgtcagcggc aatacgctct gcagcaaaaa taccgcgcat ccgcgcccag gtgttactgt
     8821 cgaccgaagc aacctgctgc ttctgcgcgg cggttaacat ctgccagagc ggatcttgtt
     8881 gccagcgttt aacaatgctc tcttcgcgat agtgggcaac cagcagccag gcaggattga
     8941 ccgccagcag ttgctccagg ccgatggacg gcatggacga acccgccatc gcagcgggaa
     9001 cgttcagccc cagagaggcc agcacgctgc cggtccaggt ctcctgagta tgcaggttga
     9061 attgctgttc gcgtgatgtg ccaaaggcca cgcgtgtccc tttgggaagc tggctggccc
     9121 actgcgccat cctctcttta tgttgttcca gacgtgcctg catctctcgc tttttaccca
     9181 ccatttcgcc gatgatagcc gcagattgca aattttcagc gtaggtttcg ttgcgggact
     9241 taagcagcag taccggcgcg atttgctgca aggcgatgta aacccccgca tggcgactgc
     9301 tgtcggcaat gatcaggtct ggtttcagag cggcaatggc ttccaggctc ggctgcgcgc
     9361 gcgttccgac ggactgccac ggtttcaggt gcgcacgcac ttcgggcagg atgcgttttg
     9421 catcgttatc gtcggcaata ccgatcgggc tgacgtccac ggcggccagc gcatcggcga
     9481 acgagagttc cagcaccaca atccgttgtg gcgttttttc gagtgtaaac gtgccgtgtt
     9541 cgtcctgaac catggcggca aaggcgtggc tgatcaccag cagcaggcct gcaaaaagaa
     9601 aacggataaa tgccaacata atcacattcc agctaaaagc ccggcaagcc gggcgttaac
     9661 acatcagaac ttcaacgacc cctgcatata cagcgtgcgc ggctggcctg catagatgcc
     9721 tttgttgttg tcgtcataag agcggatgaa gtagtcctgg tcgaagatgt ttttcacacc
     9781 gaatgctagg ttcagatctg ccatctgcgg gccaaagtca tacgccacgc gtgcacccca
     9841 gagcatgaag ccgggaatgc ggccggtact gccgtcggcg ctctctttca ccgtattggc
     9901 gttatccgca aactggctgg actggaaatc gctgttcaga ttgaacgtcc agtttcctgg
     9961 cttgtagtcc acgcccagcg tgcctttatg tttcggggag aatggtacca gattgccgta
    10021 ggtgtcgcct ttctcgcgga tttccgcgtt cacatacgca tagctggcgt agatggaaac
    10081 gttatcaagc gttggcgtta gcgtacccag atcgtaacgt gcctgcgttt ccagcccggt
    10141 atggcgcgtt ttgccacgtg cagtgacggt gtcgttggtc tggttggagt cgtactgatt
    10201 gttaaagtta atcaggaaca gccccatttc cgccgtcagc gcgccgtcgt cgtagcgggt
    10261 accgagttcc caggttcgcg ctttttccgg ttccacattg ccgctttgca cagccttgcc
    10321 aatctggctg tactgtacgg tgccgaacga gccttcagtg tttgcataaa gattccagct
    10381 gtcagtcagg tgatagagca cgttcaacgc cggaagcggt gcgttatagc tcacttcttc
    10441 gtgcgtgcct gtgatggcgt tgttctggta tgactcgata tgttcgaaac gcatacccgg
    10501 cgtgatggtc cagttgccga tgtcgatttt gtcatccaga taccaggcgt gcgcctcggt
    10561 gccggaacgc gtatcgcggt cgtaagggct tgagccggac ggcaactgcc cgctgctggt
    10621 ggcggtgtag taacgcattt catgcgttga ttcattcaca tagcgatagc ccacgcccac
    10681 ttcgtgcgcg gaagggccga tcataaagat ctggctgtag cgtggctcaa taccgcgcac
    10741 ccagtagtta cgcggcgaga gggtgatgcg tttgccttgc tccaggtagc cgctgcgcag
    10801 ggtttgggtg tagaacccct gaatgttgaa tttatgctgg ctgtctggct ggaactggta
    10861 gcccaggctc gccagcttgc gacgacccca gaagcggtca tacgggcggg tggattgcca
    10921 gcgatcggcg tcgtaatccg cgcgagacag gccaccgggc atgtcggctt caccgtcgta
    10981 atattgcagc aggctgttga aggtgtgcac ctcatccggc gcatatttgc ttttcagcat
    11041 caggtcgtcg atgcgggtgg cgctgtgctc gcgccagtca ctgccgcgcg tgccggagta
    11101 gagcagcgcg gtgccaaaac cgttgtccgc tgtgccgccc accatcaggt tgtgcgtctc
    11161 tttcgggttg ttttgtgaag aggttgggct gagctgacct tccacgcccg cctcgatacc
    11221 aaagtcctgc ggaatggcac gggtaacaaa gttcaccacg ccgcccacgc tctgcggtcc
    11281 gtaacgcacc gcaccaccac cgcgtaccac gtcaatggca tccatgttgc cgagcgaaat
    11341 gggagccagt gaaagctgcg gctgaccgta aggggcgaag gggacgggga tgccgtccat
    11401 caggacggtc gagcggctgg cgaggcgcgg gttcaggccc cggatgccaa agttcatcgc
    11461 caggtcgtgg ctgccggtgc cgttgttttc cggcgcgctg acgccaggga tgcggttaag
    11521 tacctcacgc atggtggttg cgccggtttt ggcgaaatcc tcacggcgga tcacgtcacg
    11581 cgcgccagca tgttcaaata cgtcgttttc acgcgcatca cccagccagt cgccgaccac
    11641 ggtcagggca tcttcttttg gtgcgggcgc gggctccagc gtccagctgt tatttcccag
    11701 cggttttacc tgcagtccgc tgccgtccag cagttgttgc aggccgctct cgacgtcgta
    11761 atcgccgtgc aggccgttgc tctgcttgcc gcgcgtcagg ctggcgtcaa ccgagagggt
    11821 aaatccgctg tgtgcggcat actgattgag cgctttatcg agcgatcccg gtgcgatatt
    11881 aacctgtgca gcaaaagcgg aaaacgagag accggccagc ggcagcaggc tcaggcgaat
    11941 ggcgttaacc aaaggtgttg ttttacgaaa aacgcgtaac ggcgtcatac cttccccatc
    12001 atcatttttg ttgtgttcag ctatgagtcg aacgagaagg gtaaaaagga caatcgaaat
    12061 aagaattatt ttccttacag tggtgaaatg tttatccagt accgcgtaat agactgaatt
    12121 ttaacgggaa gcgtttgcgc gataacgttc aggatcgcat cggtattttt cagcgggaac
    12181 gtcccgctca ggcgcagccc ggcaacggcg ggatcgcagc gcagcacgcc gttgcggtaa
    12241 cgggttagcg tggctatcac ctcacccagc ggtttatcgc tgaagctcag gatgtccttc
    12301 gtccagcttg tactctcgtc atccagcggt ttcactgcgc caaactcaga ggcgctgaac
    12361 tgcaggcttt caccagcgtt cacgatgcgt ttttgcgcgg gggcactggc gagaagcact
    12421 tccacagcgt gctgctgaac gtcaagctgc gtgaaattat cctgctggcg gacggtaaat
    12481 tctgtcccta aagcagtgag ctggccctga cgggtcagga cacggaaggg gcgttgcagg
    12541 gcatctttcg cggtagtaat ggcgatttca ccgtaccaga gccggacggt gcgctgatgc
    12601 gcatcaaaac gcacatccgc cgcgctttgg gtattcagcg tgagcaggga gccatcttcc
    12661 agttgctgac ggctgactgt gcctttggcg gtgcggtaat ctgcccgcag accttcgccg
    12721 gtttccgact gccagagctg ccagcctccg ccagcgccga gcaacagcag taatcctttc
    12781 atcacgtgac ggcgggtgag gcgggtatcg tgcaacgccc ggctggcaac gtcgccaggc
    12841 acaccgccaa gctggttgcg caggttttca acctgctgcc aggcccactg gttatcctga
    12901 tcctgttcat accactgttg ccagcgcgct tcctgttgtg ggctgacgcg ctcgccgctt
    12961 agcacggcat accagtggga agctgaacgc agcgcctgac ggcgggaatc ggttaacaaa
    13021 ggattcataa cccatactcc agacggaaca gcaggcagtg ctcgacggct ttcgccacgt
    13081 attttttcac ggagctgatg gaaacaccga gtttgtgcgc aatctcgctg tatgtcagac
    13141 catccagttg cgaaagcaga aacgcttcac gtgttttgcc gtttagcccg tccagcatgc
    13201 tgtcgaggag ttgtagggtc tcgagttggc tttcgcgttc ctcaggtgaa ggcgctcccc
    13261 cctccggcat aagcgccagc atctccagat acgctttttc cagcgcgttt cggcgaaaca
    13321 ggtccaccat cacgcgtttg gcgatagtgc agaggaagga gcgaggatcg cggatcgtcg
    13381 agagcgtttc gctgaccatt acccgcaaaa aagtgtcctg ggcaatgtca tctgcatcaa
    13441 aagcagactg gagtttgcgc gtcagccagc ttttcaacca gccgtgatgt gtgccataaa
    13501 gcgactcgaa cgttaaggaa gctgtggtag tggcgcggtc agacatgcgg agtgcatcaa
    13561 aagttaatta tcacgtagtc atattaatat gagaatggtt atcattacaa ttggaaataa
    13621 aattgtttcc aatagacatt tttaacatgt tgtttttcta agtgttataa ggtaggtata
    13681 aaatgggatg gagcctctgc ttctgacatg tgtcggtcag aatgactcat gatgtggtct
    13741 gctattattg acatcctcac tgccctaaag gatggggatt tcggtaatga tcttacccag
    13801 caatagtgga cacgcggcta agtgagtaaa ctctcagtca gaggtgactc acaagacaaa
    13861 acagtttcaa ccagtaaaaa aacgtaaaca ccattcgcat gaatttcgta gttaagatct
    13921 gaagcttgca gaatgcatcg gtgttactgc cggagtccgt gaacttagtc tgcatgaatc
    13981 accacagctt tacatctgtc gcagtaaaca gcaaaatcag caaacttact taattatatc
    14041 gagtgcgatt acaatcgctg gcgatggtac agttggtgtg gcggtctcag tccggaacaa
    14101 tttgaaaatc ataatctcac ttaaaacggc atccatttta caaaagatgg ccgtctgtgt
    14161 atttgtaatg aataccttct acatgtttag agtgcataat ccacgacaag gcgatatcct
    14221 aaaaattagc tagcaggcaa ctttgatggc atagataatg tttttagtaa aaaagagttg
    14281 actgaatata atacagcctc ttcctacata agaaggaatg tgctcacaaa aaaaaaccat
    14341 tagtctgctt cctgttctta caataatcag tattatgtat tttatttata tagcaaaata
    14401 tgcttgttac acctgtcata attaggcatg gtaaaattat tgactgaacc ctgggggaga
    14461 aagatgcaca gtgattatta taatatggtt tttggtgaaa aattggccaa tattttatat
    14521 gaagctaact ctcaattttt ttacgagaga aatgtcatag aagaggcagt caacgcttta
    14581 ttctgtgagc gtgaaataat aaacaacaag aacataataa agaagctgat gttctttttg
    14641 tcagatgtta atcacaccaa gaaagatgtt gttcagagcg cacttaatat tatcattgac
    14701 attacatcag gtgatatttg agattttaca taaaagataa tctctcaagg agtcttatct
    14761 gaaaacgaaa tatatagtcc ctctcgtttt gtaatatcga ttggctttgt tgaatcggtt
    14821 acgtaccccg gagccggtca tattttcagg taatacacac gctttcagac atttcggctt
    14881 tcgtcatttt tgtatcatgg gcatagcttc tgctacctga ccatcataat cacgcagctt
    14941 cagtgaagca gtgaccacct gtttaacccg gtgtatcgtt atgatcttat ccagcaattg
    15001 aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc agatcatcgt
    15061 ttccgatgga agcataataa gctttttctg cttctgccgg tgggatatgg ccctgccttt
    15121 ccagcaatcg tcgattgtta taccagtcca cccacgtgag tgtggtcagc tcatcatact
    15181 gacacgtgca ttacccctac cgcgataatg caaccgtaca atatagggcg tttcctaaac
    15241 gcagccctca tcactccaat ccaacaagac acagcatttt tgatcacgaa gaaagacaaa
    15301 taatttccgt aaatgtttcc gttgttacaa ataacaacag tgttttacat ttctttggcc
    15361 tcaggcccgc gcatgttgcc attcgatgtc attttcctga ctcgtttctt ctttatggtg
    15421 tagtgtgatc ttcactttat agatgctaac aatatgaaaa gaaaaattat tcccgtgcta
    15481 atcggttgta cgctctcttt ctctgccctg gcggcacaac ctacggctga gcgctatgtc
    15541 gtcagttttc cagaaggcac gcacgtgaac tacgccggcg catttgccag tgcattcccg
    15601 aacggccttc cggttgggat aggttctggc ctgctgttca ccggtaaaca gggcgatgcc
    15661 ctgacattcg caaccattac cgatcgcggt ccgaacgcgg attcaccgaa agaggggaaa
    15721 aacgaaacta aaattttcgt tacgccagat ttcgcgccgc tgctgatgac gattcgtgta
    15781 caaaacggca aagcggaggc catcgatccc cgtcctctgc atgacgataa aggcgcgatc
    15841 aacggtctgc cgctggcaag cgacgtgatt ggctccacca atgaagtggc cttcagcgac
    15901 acgttacata gactgaaggg ggataatcgc gggctggata cggaagggat cactccggat
    15961 ggtaaaggag gttactggct gtgcgatgag tatggcccgt tcctgattaa catcgacagt
    16021 aaagggaaaa tcctggcaat ccacggtccg caggcggcgg aaggggaaaa agcgattgcg
    16081 ggcggtttgc caaatatcct caaatggcgt caggcgaacc gcggctttga aggcctcacc
    16141 cgtatgccgg acgggcgtat tattgtcgcc gtgcaaagta cgctggatat cgacgctaag
    16201 agcaagaaaa aggcgctgtt tacgcgtctg gtgagcttcg atccggcaag cggaaaaacc
    16261 gcgatgtacg gttatcctat cgacagcgcg gcctacagca aaaacagcga cgctaaaatc
    16321 ggagacatcg tggcgctcga taaccagcac atcctgctga tcgaacaggg aagagataaa
    16381 aacaacagaa tgcgtaacct gatttacgag gtcgatctca ataaggcgag cgacctgtca
    16441 ggcttcgaca agccgggtga gtatccggag tttgatgatg agaaaaccct gtcacagcgc
    16501 ggcattaccc ttgcgcagaa aacgcaggtt gttgatctgc gctccttggg ctggcagcag
    16561 gagaaggctg aagggttggc gctgatcgac agcaaaacgc tggcggtggc gaacgataac
    16621 gactttggcg tgaaggtggc gatgcaacat ccggtcgagg gcaagacatt caaggattat
    16681 cgggtgaacg cggaaggaaa gctgacgctt gacgacaagc aggttgaaac gaccctcaga
    16741 gtgaagccac tggagaaacc ggagtctgac agtgaactgt ggattgtgac gctgccggaa
    16801 gcgttgaaat aagtttataa ggtacggaca gcagtatcgc ttagtttttc aaattcatgt
    16861 atgtgaacaa tattggggtt ccggcgtaga cccccaatga aagcgtcacc acgtgaaaac
    16921 atgcctgcac ggtttacacg tcatcccatg gtggcagccg gttaactttc ctgcgccacc
    16981 agtggaaaaa ggccataagc agtccgaaca aaagtaagcg tacagcgagg gccgtattga
    17041 cggggatgtg ttattcagct ggcagtgcta tgcgccacgg aagcagttcg ctgacccggt
    17101 tgaccggcca gtctgctatg acgccaagca catggcgaag gtagctttct ggatccacgt
    17161 cattcagttt gcacgtcccg atcaggctgt acagtagcgc tccccgctca ccaccatggc
    17221 cagagccgaa gaacagggtc ttcatctttt cacgcaacca gctttccagg gatttcaaca
    17281 gcggtttcgt ttttcgctga cgttcagcaa gccgctgctc tgccggcatt ccccttatat
    17341 ccgcctctat ggcgtacaac tgaccgatct gctccagggc ttcttccgtc agtgctgacg
    17401 ggatgcggac gtgcacatcg tggatctttc ggcgggcatg agcccagcag gcagcttccg
    17461 ttatcccacc attgcgatac agctcgttga acccggcgta cgcatccgct tgcagcacac
    17521 cgctgaagca agcaagatga gtctgcggat ggatgctttt tctgtccggg ctgtaagcga
    17581 accacactgc aggtgccaac gctgacccgg cattgcggtc atcacgaaca tacgcccaca
    17641 accgcccggt cttcgtcttc ttattacccg gcagcagtac ctggaccggg gtatcatcgg
    17701 catggagttt gccgtcagtc atgacatagc catgaagcgc ctcttccagc ggagacagca
    17761 gccggcagca tgcatccacc cagcccgaca gcagtgaacg gctcagctcc acaccttgcc
    17821 ggccgtatat ttctgactgg caatacagcg gggtgtgctc tgcatacttc gaggtcagca
    17881 cgcgggccag cagccccggt ccggcgatac cccgctcgat gggccgcgaa ggtgcaggtg
    17941 cctgcacgat ggtatcgcac tgagtacagg catgtttttc ccgtaccgtc cggataaccc
    18001 ggaaggcgct acgcatcaac tccagctgtt cggcggtatc ctcgcccaga tagctcagtg
    18061 aaccgccgca gttcgggcag cacggcgccg caggcaacag tcgcttttcg tcacggggta
    18121 gtgattcagg gaacggctta cgggtgcggg tctgacgcaa cggacgctgt actgccgggt
    18181 catacaccct accagtcagc gtatcgctct ctttctgaag ccggttcaga tcggcttcca
    18241 tttgtgcgat acggcgggag actttttcgg aacgactgcc gaagttcatc cggcggagtt
    18301 tatccagctg cgcctgcaga tggtctattt cgcgctcccg gttgctcagc ttttcctgca
    18361 gggcgtggat cagcgcttcc tgttcggcca ggcgctgttt cagcaggaag atgtcgtcag
    18421 aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa ccaggataaa
    18481 gagatttaca gcatggtcag ggaggtcagc agccgcttgg gctgtcgcca gtcgatacct
    18541 tccagcagca tcgccagctg cgcctgcgta aggaacactt tgccatcacg ggctgacggc
    18601 caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc gccggtggac
    18661 cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaatatg gccggacatg
    18721 ggatcgtctt tcagcgccgt ttgtactttc gcagccaggc cgttgaagct atttctcata
    18781 tcggtgatac cggcaaccag ccaaattttg gtcccggaag gtaacgggat catcgcttca
    18841 gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag cggagcgtcc
    18901 cgtgccggaa cgttacctca cagctgatac tgagggtttc cgggtcctct gcgagcgatt
    18961 ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc tctgaagaag
    19021 gtaatagcag ctttccctcg cgccattgtt gtcgccattt gaacaacaga ttggcgttaa
    19081 tgccattttc aagagcaagt tttgagatgg atatcccggg ttcacaggag gcagcaacga
    19141 gcagctgttt aaattcggga ggataattag ggcagccttt tcgcctgccg ggagtcacat
    19201 ttttctgcat atctgacact ttggttccca ctacttattt ggtggacacc actttgtcta
    19261 attcgtcaga ttctgaccag acggttcagg ctgtacgctt accactattt tatccctgtt
    19321 gaatttggtt aagccggtgc agacgcgccg ggaaacgcac acctgaaccg gcacaataaa
    19381 atacaataca accccctgtt actggagata cgaatggaat gacgaggcag cctgtcagtc
    19441 acagggggct gttggcataa aatcaacgga aaggccacca acaccggaat taagctccac
    19501 agtccccgca ctggcaatcc attcccggga aaggcatcac cgcaaactgc tccggaggca
    19561 accattacag cctctgtaac ggattatcag ccctggcatc ttccgacggc acctcctgcc
    19621 gcccccacgc cctgctgtat accccggtac cgcagcgtgg gcagcacttt tccccctcat
    19681 aatcttcgtc acacatcaca caaaaccact ttctcacctt caccgtataa tccggaaccg
    19741 tcatgccctg cagatgcaaa cgacgggcct ctttctctgc cgggtcaagc caggtacatc
    19801 gcagcaggat tttttcagcg acagtcatct ggtcatgttc aaaccacggt ggttgtccgt
    19861 cattgccccg ctgaagtgtt aacgggcatc cacaggacac acagcacaaa acgtcctgtc
    19921 ggttgtgctt cgcagcttct ttcgccgtaa caaaatggcc tttgctgtca ttcgccatat
    19981 aacagttcag aattcgcata tttcctcctg agttgtccgg ggaatactga atcctccggt
    20041 ctggcagtgt gtgcagtact gctccccata ataatcgtgg tgacattgtc tgcagtgcca
    20101 gctggcttta cgcaccacgg gtaaggcatc cggtacgaat tgctgcagac gtttaatcaa
    20161 ctgtatttct ctgcgctccg gcctgacata agggcactgt tgaccgtgct ctgtcagcct
    20221 gtcgtcagtg tgttcaaacc agggaagttc agtttcgtat tgcggatggt atctgagtgc
    20281 actgccacac aagtggcagg tgtagttagc ataaggtgcg gcctgtgcag tacgggcgcc
    20341 cgtcagacgt ccgttgccat caagagcgat aaaggatttt gcgtacatag tcatatacct
    20401 cagccgccag acgacacgca ggcaacagcc gtcccggtac gggcagcgtg gtcagggtgt
    20461 gaatggcggt tcagttaaaa tctggactgg agtttgggta aagcaggctg gttgctgtta
    20521 cagcactgtg gttgtcacac aatggaacac tgacgtaaaa aacagtcagt gggcgagtgg
    20581 atacatcgtt gccgccagac aacaacccat acttttggtt tgccggatat tggcgcgcca
    20641 gtcgtgctca actccggcta cagccaaaat tgcgctcacc ggtgcagtga gctttggtat
    20701 gctccttcgc cagatagtca gcacgttcca gcacttgctg aaagccggtg tcatcattac
    20761 gcgccagcca caccgccggc caggccggaa aatgcaccag cgtggcatgg cacccgacat
    20821 ccactcccag ggcactgcac cacgcctgtt taatcatccc ggccagagac tccggagcgc
    20881 ggtaatcacc ggcacgacac caggtatccc ggttgaccag cagcatcagg tgatagtgtt
    20941 ttttaccttt gcactcccca aactcccggg cccagacgta atgcagggtt gtgggatgca
    21001 cgcgtttacc ttcccgatgc ttacgtttct ggtaagcgtc gattcgggct ttcagggcat
    21061 tgatgaagcg ggatatcaca gctgcgtccg ttgctgccgg tacatccggg agacgcaggt
    21121 caacccgcaa tgccgtcagg cggggatgaa cattcagtgc gtgccgcacc gtctcacgaa
    21181 tacgttgctg ccagaagggg ttgtatttgt aggtcatggt taaatctccg tatggttcat
    21241 acggaatagc cacgtcgtaa aaaatacgca gagtccctga agtgacctcc ggcaggacac
    21301 tgtctcaggc actctgtgat aacccgaacg gtcgtctccg gactgacggt tgaattttca
    21361 gcgtcgtttt ttaatgtaaa aaaccggtta cgggtaaggc tgtgaggagg gagtgatggt
    21421 gatacgcaaa aagaagtgca gggactgtgg aaacgcgatt actcataata cagtgtgttg
    21481 cccgtattgt ggttctgtcg accccttcgg ctactaccgg aatacagacc gtatcgttac
    21541 catactgctg gcgctgatta ttgtggtcct gctgactact gtcagcgtca gtgtgtgtat
    21601 actgtgtagc tggtgagata acggttcatg tatcccctga gctgacagtg taccgtgtat
    21661 attaagcgta accgacaggt tctccttcat accatattaa attttccagg gattgccgct
    21721 gccttatttc gtcccataca ggaaaactca tctgggtttc tgacaaatac gcgaaactca
    21781 cagcccctca atatcaacca cacgttgtaa gccggtaata cctgaaaatc agcctattac
    21841 cgcccacgcc tccggcacca ttcccttttg atttcgaatt ctttgttgtc gctataccgc
    21901 ttaatgttcc cggtcctccg gtgcagataa caccggagtc atcgtagcgc tgcttcaata
    21961 ttcaggtgac gtatttccag acggaagccg gcgcgcgatc gtaaagtttg ttcattgtca
    22021 gttctgccag ccggttgtca acggcagagt taaaggcttc cagctccgca agggtaagtt
    22081 tataacgcgt gtgggaattc acttttcccg cgtctcctga cgtattacgg gctgccactt
    22141 acctggtgtc ccgcaataca gccttagtgt tccgttatcg gggatccgga cgcaccgcaa
    22201 caaccgggga agtatttctg gtttagagca gtgtcaagag ggatttctca gcccctcact
    22261 caccggctca gtctcagggc aggagcatta atacgctcag ttcatgtctt atacaggaat
    22321 gggcacaggg ggagctggcg tgtcactgct gattttacgc atcagcgttc atgtatctgc
    22381 actctgatac atgaacgatt cacagcgctc tgttaccgga gagatttact ctcttagctt
    22441 actctggcaa atcctttacg ttactctctg atgacttctt atatttataa taccaatcag
    22501 acagccttat cccatcggtg atatcagtct cctcctcgcc aggaacgggc tgactcactg
    22561 taactacctc cgaaaacaac tctgcaatta tcagaacgcc tgcttctctc cctgtcctca
    22621 cgaaaactat cccctcttta tcgcgcgtgc gtgcggaagc atcttttcgc aacaaccacc
    22681 cggtataccg gtacggctca gccaccgcaa cctccccgtt tatctcctgg cggtcacatt
    22741 cccgattatt ttttacgttt ctccccggtt gttatgccgg tgaaggtggc gcgctgtttt
    22801 catgaccaca ccggctggtt taacaacatc cggaggaaca ttctcatggc taccccagtt
    22861 tcactgatgg atgaccagat ggtcgacatg gcatttatca ctcaactgac cggcctgaca
    22921 gataagtggt tttacaggct catcagggat ggggcctttc cggctcccat caagctgggc
    22981 cgcagctccc gctggctgaa aagtgaagtg gaagcctggc tacaggcgcg tattgcacag
    23041 ccccgcccgt aatttctgac cgttatccgt tcacccgcag cagacgtaat ccccggcctg
    23101 ccggcggcat tctgctgcct gttatatccc cgtgaggaat atgaaaatga aacaacagta
    23161 tcagacccgc tacgaatggc tccacgaaag ctaccagaaa tggctgaccg gcttcacccg
    23221 gcacgccgta tcctggggcg tgtgtcatcc gaatatctac tacttccata atctgacgcc
    23281 cggttggggg tcattcaatg gtgaaaaacc ggagattgcc atcgtacccc agagcctgca
    23341 ccggctgatt tatggccctg acaaacgggc cacgccgccc ctggatgatg atctgatagt
    23401 gaatttatgc accagtgaac atctgctggt tcatcatccg atgctggaag gcattctcct
    23461 gtctgaatgt gaacgtctga ggcagcgttc tctggcaaat aaactgatca gcctcttccg
    23521 tcagtttggt ggcacggagt tgcgcctcaa actggtctgg ctttgctggc ttgatttaat
    23581 gaccggaaac agccttgaag actggaaaga gaacctgaaa cggaaatcag aaaaagagct
    23641 ggaggaatgg atcatcaacc gtcagaggca gagcgcagca ctgacggatc tgatggatca
    23701 gtacgtgctc ctggcatacc gcacaacggt tgacgatagc cgcaactgat gccggttgct
    23761 tccggaaggt gtgacaaaat acggaaatga tgatgctccc ggcccccatg ctcacctgca
    23821 gagcgtgttc gtcgtcatca tactgagcca tgacgggcgt cttaagggca tcctgtcatt
    23881 gctgagcagg caacaccacc agaggagcca cggacaggga tgttctttac agcccgatac
    23941 acgccaggtt ctcagcagac tgaatgtgca ggtgcggtta ttcaccagaa atggcatgct
    24001 gcggtacata cccatcctgg tgacaatatg tacgtggcga cagcgatccc ggcactgtgg
    24061 aagagactca ctcagcctgc aggatattct ttccggcatc cgggaatgtc cgcctgaatg
    24121 caaacaggat gatatttgaa tccgtaatac tgttctttca tcataaataa tttatgcaga
    24181 tacagttaac cgttacagta gccggtttgt tttaccggga aataatatcc gtcagactga
    24241 taccggaaca tatctgaaac acgttatgat gttttgttat cagaaatgtt gtatattctt
    24301 atccgtggcg acagtttaat gccctctggt atcacctgat aatgtatatc ctgcgatgat
    24361 tcgttatatt tacgggacac gcctcttttc attgtgctcc ttttcagact tcattttccg
    24421 taagtgttcc ccggcataaa ggaggtacct ttctggcgtt tataactctc tttatcggtg
    24481 acgccggtaa tacagggaat accacaccga caaacgaggt attaacagac gatatttcag
    24541 tgtccataat ttttctgtcc ggttaaataa cgtcagtctg ctgccctctg actgtaatca
    24601 tcaaagccgg aatgcatacc gggttacaac gtaaaaaaag tgatgctggc ccccggtgca
    24661 gctggcgggc gaaacatgtt tacctttcaa taccccctgt ctgcagtctc cactcttcca
    24721 tcctcttaag ctacgtcggc cacatgagtg cacttccatg ataacaggta ttcctgtcag
    24781 cataacgaag ccggttacct tcccttcctc ctgcatttcc tgtcagaatg actatacagc
    24841 gcgcagattg cctttttatt gaccatctta atataccgtt ataaacttct ttttatcata
    24901 ttattatcac cttattatgt ttaattttta agcagtcgga gaaagaggat ggttcagaaa
    24961 attttgtcgg ataaggttat gaatgaaaga acaaatgcgt attactctta ttatcttgga
    25021 gagcgtaata tctccgtact gccccttaat gtttatgatc ccccggaacg ttttatcgct
    25081 tacataaaaa aaaacagaga aaatctgaac atcacacttt cagattttga acttgaacaa
    25141 ataatttctg gtatgcgtct gaaggccctg gccttcctgg tccccctgga aaaaatatcc
    25201 tggatcgcgg gcagtgaacg cgcatgctta ttttcctggt atctgttgat gcagtttatc
    25261 cagaataaca gggcaaaaat aagcgcagac ttgcttcaaa agaataaact gtatcttaaa
    25321 gaagagtatc tggaagggaa tgccttcccg tcggactcat cgacacagtt cagacaaata
    25381 ctcagagttc tcgatattct ttctgataag aatttgcggg atgagtggat aatccagacc
    25441 aaagacagat ggatgcgggc atttaaatca aagagtcctt tcagttacct cctcccggag
    25501 aacgaacatg aatgcatatg gacatggaat tatttgaagg gaaaaaatat tgcactggaa
    25561 aaactggcca gttttcctgg ttctgctgat atttaccacg caattcacct ttcatttgac
    25621 atatgggtta cctgtccact cacctcacct gacgatataa aaaatttcag aaacagtttc
    25681 aataaggcca aggcgcagcg caagtacaaa aaaatgcagg aagataaagt caatgtacag
    25741 ttttttcttg acgcagaaac cagggcgcag ctcaaagaat tatcgagggt cagaagactc
    25801 agtaccggcg aaatgctgca tgacctgatt gttgaagagt ataaaaggta ccgacattcc
    25861 agataatttc agacatatat tatctccgtg agtcccccgc cacctttccg gtgtgcgggg
    25921 gttttgtctt ttttcaccgg gaatacatgt atgaatccgt ctgatgccat tgaggcaatt
    25981 gaaaaaccgc tctcctccct gccttactca ctttcccgtc acatcctgga acatctgcgc
    26041 aaactcacca gtcacgaacc cgtgattggc attatgggta aaagcggggc cggtaaatcc
    26101 tcactctgta atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc
    26161 acccgggaag tgcagcgttt ccgtctgagt ggccatggtc acagcatggt tatcactgac
    26221 ctgcccgggg tgggcgagag ccgggacaga gatgcagagt atgaagccct gtaccgtgac
    26281 attctgcctg aacttgacct ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct
    26341 gtggatgagt atttctggcg acacatcctg caccggggac atcagcaggt gctgtttgtg
    26401 gtgacgcagg ccgacaaaac ggagccctgc catgaatggg atatggccgg tattcagcct
    26461 tctcccgcac aggcacagaa tattcgcgaa aaaacggagg cggtattccg tctgttccgg
    26521 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
    26581 agtgcgctaa tgacagcact tcccgaccat gccgccagtc ccctgatgac ccgcctgcag
    26641 gacgggctgc gcacggaatc tgtccggggt caggcccgtg aacagtttac cggtgcggtg
    26701 gaccggatat ttgacacggc ggagagcgtc tgtgttgcct ctgttgcccg taaggttctg
    26761 cgcgctgttc gtgacaccgt ggtctctgtt gcccgcgcgg tatggaactg gattttcttc
    26821 tgaacccggt ggtgatgcac cagactcttg ttacagattt ttaaccccat gaaaacacaa
    26881 tgcaaacaca gccatacccg ggttgtcccc ttgcgttgtg tttctcaagt taatagaatg
    26941 catcgatcgt tactatcata tcgatcgcta taaacgatcg tttgtggtaa atgacctcct
    27001 ccgtggttgt catgaaaatg cgtctcagga aggttaaata atgcacgaaa agaacatcgc
    27061 cctgctttgt gatgaagccg accgactttt gcaactgaac attaatctgc tccggcaaat
    27121 ggttgatgaa ccggatgtgt tatctgacag taagaacgaa aacagactgc tttttgataa
    27181 acagaaagca ctgaaaagaa ttgaggagct ggagggcgaa caaatcaaaa ccgcccgcag
    27241 ggagatggtg ctggctgttg tcggcacgat gaaagcaggt aaatcaacta ccatcaacgc
    27301 cattgtgggg caggaaattc tgcctaaccg taaccgcccc atgacctctg taccgacgct
    27361 catccgccac gttcccggaa aaactgagcc ggtcctccat ctggaacata ttcagcccgt
    27421 ccgcaattta ttaagcacac tgcaacaaaa actcgacacc ccggcaggac agcaagtcgc
    27481 acagaccctg cagcaaaccg gggatacctg cgaactgctg gatattctgg caaatgatgg
    27541 ctggctcaaa aatgaatacc acggggaaga cgaaatcttt accggactgg catcgttaaa
    27601 cgatctggtt cgccttgctg cggcaatggg gagtgagttt ccttttgatg aatacgcaga
    27661 agtgcagaaa ctgccggtga tcgacgtgga attcagccat ctggtgggga tggatgcatg
    27721 ccagggaaca ctcacactgc tggatacccc cggccctaat gaggccggac aaccgcagat
    27781 ggaagtgatg atgcgggatc aactgcagaa agcctctgcg gtgctggcgg tgatggatta
    27841 cacccagatg aactcaaaag cggatgaaga cgtccgtaaa gagcttaatg ccattgctga
    27901 cgtatcagcc ggccgcctgt ttgtgctggt caataaattt gatgagaaag accgcaatgg
    27961 cgatggggca gatgccgtac gccagaaagt tccggcaatg ctgaacagcg atgtgctgcc
    28021 cgcctcccgc gtttatccag gctcatcacg tcaggcgtac ctggctaacc gtgcgcttca
    28081 tgagttacgg aaaaacagaa cccttcctgt tgatgaagcc tgggtcgatg attttgtcag
    28141 ggaagccttc ggtcgcatga aaaaagaata cgtctgtaaa gacagtgaaa tggcaaccga
    28201 aggggcgaca gacctgtggg aaggctcact tatcgatcaa ctgataacgg aagtcataca
    28261 gagctcccat tccagagcgg cggcactggc ggttgattct gccgccgcaa aactgatgca
    28321 gaatgcggaa aatgtcagcg aatatctgtc attacgccat caggggctac agcaaagcat
    28381 tcagtcactg cagtcgcata tcaccagcct gcttgcggat atccgggaaa tcgcggactg
    28441 tcaggagcag gtgaccgctg atgtcagaat ggccatggag gagatcgatg ccagaacccg
    28501 ggaattactg acgggggtct gcacctcact ggaagaggag ctgaatgact atttcagaag
    28561 cggtaaacgc aaagaacagc aaatgctgga ggaagaaaat tcagcgcagc cccgggagag
    28621 aaacgcattc gctttctttc acgatatttt cggaaccgga aatcagcatg acaggatgcg
    28681 ggactttgat ccggacagcc ccgaaataaa attcagtgat cgcagggaag cccttgaact
    28741 gatgacgcaa atcgaatcga ccgtgaccag cctgcaccgt gaggctgaag cacagttccg
    28801 tcctgaactg gaaaaaatcg tcaggggtat tgaaacaggt tttcgtggta cggccctgta
    28861 cgccacagaa aatattgccg gacgcatcaa tgcccgtctg gaggatgaag gtttcactgt
    28921 aaaaatcagt tttccggcag tcagccagtt acagacccgg ctcgcagtaa aaacaaatct
    28981 gagcgcgctt atggaggaaa gaacggagac agtaacccgt cgctgtcgac aggatggtgt
    29041 ctggggtacg ctttgtcgat gggcaaacac cagtgactgg ggctggaaag agtacagtgt
    29101 ggatgtgagc cgcagcgtga tcaatatcaa caaggtcaga gaggaagtga tgtcactgac
    29161 ccgggcatat ttcggggagc tgcaggcatc cattgagcag gatattaacc agcccgtccg
    29221 ccaggaaatc gatgcctttt tctgtacatt cagggagaaa gttgaacagc tgcgtaacac
    29281 gctgattcag agctctgaag atcataaacg cgatcagcag gcgcaggaac ggctgaccgg
    29341 gcgtcttcag gcgttaaatg aaagggttcc tgagctcatt actgacagta aggcgttaag
    29401 ggaagaactg gagacaatgc tgtgacttca ccatttattc aacaaattgc tgataaccgg
    29461 gtatgtcagg tactcacctg tcttcctgaa aaatttgtgg ttgatttcgc aaacggtatt
    29521 gatgttgcac aggagcatat ccgtacggcc ggagagcgca cgttcttccg gcggttaaaa
    29581 gaggggctga gcggcgaagg tactgcacgg cagaatgcca tcaatgcttc gcttgcacag
    29641 ggcgttgagg cgtccctgcg ctggctgacg gagctgacaa cgtcacttgc caccacaaac
    29701 tacgcgatta cccgggtaaa cgacagggtc agttcactgg tcagtgatac tgccaggctg
    29761 gcgcattatt ccgcagatac gcgggagcag ttactcaccc tggccgatca ggttcaccag
    29821 aaactgaatc atcttgaaga aaaactccac cgtgttgacc tggttcagcg ggcgcagtta
    29881 catcttgagc agatattctc ctggtggagc gccgggcgat atgcgtcatt ttcccctgcc
    29941 ggacgttgtt atgtggcact tgaagagctt cgctggggcg cgtttggtga tgtgatacgt
    30001 cagggcgaaa caggccaggt taaccagcta ctggatattc tcagacataa agcattaacg
    30061 cagatggcac aggagagtgg cggtagtgca accgtgcgtc tgaacactct ggactggctt
    30121 ggtggtcagg gccgggagca ggcagataac gagtggcatg acgcgattaa ctggctgggg
    30181 gactggtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
    30241 ttaccggttc gcatgccccg tctctgctct gcagaacgcc tctctgaaag tatggtcgat
    30301 gaaatatttc agaagggggc tgcatgagta tagaaataaa aacagggctg gtgctgtccg
    30361 gcggtggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca gagtgtggta
    30421 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcgg
    30481 cctctcccga tctgtcagaa gctgccgtac gcctggaggc gctctgggat catcttggga
    30541 ataatcaggt gctgtcggta aaccgatcgg tttacttttc attgctgaaa aaattgttcc
    30601 aggcaatgaa cctctgccag atccccggac gtgcaggagc actgcttacg acacttcttc
    30661 gccatatatc gataatcaac ggcttcgaca atccgatgac tcagccgttg ttgtcagatg
    30721 ggcccctgac agcgttgatg gatcattatc ttgatacaga tgctctggca ggcgggctac
    30781 cgctgtatgt gtcgctgtac cccacagaag ggggcatgca ggatattatt gactgcattc
    30841 gtgctgaact gggttccgga accacgaaaa acgccgtttt tcagcatatc cagagtctgc
    30901 cccgcggaca gcagaaagag gctctgcttg cgtcagccgc actgcccctg ttgttccgtc
    30961 cccgtgaggt tcaggggaca atgttcggtg atggtggtat gggaggatgg cgaaatatgc
    31021 agggaaatac ccctgtaacg cctctggtcg atgccggatg caatatggtg attgtgtcgc
    31081 atctgagtga cggttcttta tgggatcgcc aggcttttcc ggacaccaca atccttgaga
    31141 tccgtccccg gaaaaggctg aaatatgcag gtgatggtgg caacagtggc ggtctgctca
    31201 gtttcacgtc ggcacatacc gacgcctggc gtcagcaggg ctacgaggac acgatgctga
    31261 cgatggagca tatccggaaa ccgctggcag cacgtcaggc actgtcccgg tcagaaacgg
    31321 tgttgcagaa aagtctggag ataacggaag aggcagattt ggcactgaga agcgcgatgg
    31381 cccggattaa ataaagacgc cccaaagaaa cgccaccgcc tgtggtggtg tttttcctgt
    31441 ctggtgttgt tgaaaatatt ttcagatata tattacctgg gtgagattta agattaacgc
    31501 atttattaat gtcataagga gagttatcat gaaattcatc gtaaaagcac ttaggtgatg
    31561 ctgccaactt actgatttag tgtatgatgg tgtttttga
//
LOCUS       DAJVRP010000043        31421 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVRP010000043 DAJVRP010000000
VERSION     DAJVRP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.043"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..1013)
                     /gene="yeaG"
                     /locus_tag="OQN12_002000"
     CDS             complement(<1..1013)
                     /gene="yeaG"
                     /locus_tag="OQN12_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1654327.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISE"
     gene            1449..2195
                     /gene="mipA"
                     /locus_tag="OQN12_002001"
     CDS             1449..2195
                     /gene="mipA"
                     /locus_tag="OQN12_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1654328.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2285..3046
                     /locus_tag="OQN12_002002"
     CDS             2285..3046
                     /locus_tag="OQN12_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS1654329.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3097..3981)
                     /locus_tag="OQN12_002003"
     CDS             complement(3097..3981)
                     /locus_tag="OQN12_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1654330.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4065..5060)
                     /gene="gapA"
                     /locus_tag="OQN12_002004"
     CDS             complement(4065..5060)
                     /gene="gapA"
                     /locus_tag="OQN12_002004"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1654331.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5402..5815
                     /gene="msrB"
                     /locus_tag="OQN12_002005"
     CDS             5402..5815
                     /gene="msrB"
                     /locus_tag="OQN12_002005"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1654332.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5857..6129
                     /locus_tag="OQN12_002006"
     CDS             5857..6129
                     /locus_tag="OQN12_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1654333.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6499..7575
                     /locus_tag="OQN12_002007"
     CDS             6499..7575
                     /locus_tag="OQN12_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1654334.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7602..8981
                     /locus_tag="OQN12_002008"
     CDS             7602..8981
                     /locus_tag="OQN12_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1654335.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            8998..10043
                     /locus_tag="OQN12_002009"
                     /pseudo
     CDS             8998..10043
                     /locus_tag="OQN12_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10064..10900
                     /locus_tag="OQN12_002010"
     CDS             10064..10900
                     /locus_tag="OQN12_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCS1654336.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            10905..11852
                     /locus_tag="OQN12_002011"
     CDS             10905..11852
                     /locus_tag="OQN12_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCS1654337.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            11862..12842
                     /gene="ydjG"
                     /locus_tag="OQN12_002012"
     CDS             11862..12842
                     /gene="ydjG"
                     /locus_tag="OQN12_002012"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCS1654338.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            12979..13737
                     /locus_tag="OQN12_002013"
     CDS             12979..13737
                     /locus_tag="OQN12_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1654339.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            13855..15213
                     /locus_tag="OQN12_002014"
     CDS             13855..15213
                     /locus_tag="OQN12_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1654340.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15306..15947)
                     /gene="pncA"
                     /locus_tag="OQN12_002015"
     CDS             complement(15306..15947)
                     /gene="pncA"
                     /locus_tag="OQN12_002015"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1654341.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(15958..16974)
                     /gene="ansA"
                     /locus_tag="OQN12_002016"
     CDS             complement(15958..16974)
                     /gene="ansA"
                     /locus_tag="OQN12_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1654342.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17141..18997)
                     /gene="sppA"
                     /locus_tag="OQN12_002017"
     CDS             complement(17141..18997)
                     /gene="sppA"
                     /locus_tag="OQN12_002017"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1654343.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19158..19709
                     /locus_tag="OQN12_002018"
     CDS             19158..19709
                     /locus_tag="OQN12_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1654344.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            19826..20869
                     /gene="selD"
                     /locus_tag="OQN12_002019"
     CDS             19826..20869
                     /gene="selD"
                     /locus_tag="OQN12_002019"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1654345.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            20874..22835
                     /gene="topB"
                     /locus_tag="OQN12_002020"
     CDS             20874..22835
                     /gene="topB"
                     /locus_tag="OQN12_002020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1654346.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            22962..24002
                     /locus_tag="OQN12_002021"
     CDS             22962..24002
                     /locus_tag="OQN12_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1654347.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24119..25462)
                     /gene="gdhA"
                     /locus_tag="OQN12_002022"
     CDS             complement(24119..25462)
                     /gene="gdhA"
                     /locus_tag="OQN12_002022"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS1654348.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            25698..25970
                     /locus_tag="OQN12_002023"
     CDS             25698..25970
                     /locus_tag="OQN12_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1654349.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWWRVKP"
     gene            complement(25936..26343)
                     /gene="nudG"
                     /locus_tag="OQN12_002024"
     CDS             complement(25936..26343)
                     /gene="nudG"
                     /locus_tag="OQN12_002024"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS1654350.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRATRAAD"
     gene            26430..27050
                     /locus_tag="OQN12_002025"
     CDS             26430..27050
                     /locus_tag="OQN12_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1654351.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27059..28366)
                     /gene="ynjE"
                     /locus_tag="OQN12_002026"
                     /pseudo
     CDS             complement(27059..28366)
                     /gene="ynjE"
                     /locus_tag="OQN12_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28433..29086)
                     /locus_tag="OQN12_002027"
     CDS             complement(28433..29086)
                     /locus_tag="OQN12_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1654352.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29086..30603)
                     /locus_tag="OQN12_002028"
                     /pseudo
     CDS             complement(29086..30603)
                     /locus_tag="OQN12_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30576..31421)
                     /locus_tag="OQN12_002029"
                     /pseudo
     CDS             complement(30576..31421)
                     /locus_tag="OQN12_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7874 a         7523 c         8151 g         7873 t
ORIGIN      
        1 tcttcggaaa tgcgcaagca atacggcacc ttcacgatgt aaacacggtc gaggaaggct
       61 tcgttgtttt tgttattacg gaaagtgacc cattcggact tgttcgagtg ggcgagaata
      121 atcccgttga acggcagggc ggagatacct tccgtcccgt tgtagttacc ttcctgagtg
      181 gcggttaaca agggatgcag cacttttatc ggtgctttaa acatctcaac gaattccatg
      241 atcccctgat tggcgcggca cagcgcaccg gaatagccgt aggcgtccgg gtcattctgc
      301 gcgtagtgtt cgagtttacg aatatcgact ttcccaacca gcgcggagat gtcctggttg
      361 ttctcatcac cgggttccgt tttggcgata gcaatttgtt gcagaattga cggccagacc
      421 ttcactaccc ggaacttagt gatatcgcca ccaaattcat gcaggcgttt tgccgcccat
      481 ggcgacatga tggtgccgag ataacggcga ggaatgccat actctttttc cagaatctga
      541 gcatcttcct gcggattgaa aagacagaac ggatgatcgt tgaccgggct acgctcaccg
      601 ttggcgctca atacataaat cggtacgagc tgcattaatg atttcagtcg ctcagcaagc
      661 gatgatttac ccccacccac aggccccagc agatacagga tttgtttctt ctcttccagc
      721 ccctgagccg cgtgtttcag ataagagaca atctgttcaa tcgcgtcttc catgccgtaa
      781 aactcttcaa atgccggata acgtgcaatg acccggttag aaaagagtcg agaaagtctg
      841 gggtcctggg ctgtatcgac catgacaggc tcaccgatag ccatcaatag ccgctcagcc
      901 gcgttggcat aagcactgcg atcttgccga caagtggtaa gaaactcctg cagtgtgaac
      961 tcttcgtcct tggcagcttc atatcgctgg cgatagtgat caaatatatt catggtatgc
     1021 cgtcctttcg ttttttagca caggttaaga gccgttcata tgaatagtag aggctcccgg
     1081 aagaggtaaa ctgaacgacg tctcttgcta aaacacagca actctcatgc cattttatcg
     1141 acgatgagaa caacgtgccc ggtgatacac cttcaaaatt aagcgtagat ggcaaatgga
     1201 aaacttgcct gcacggcttt cttaatttca atggcatatc aacaactcat ccataaattt
     1261 tgcataatta atgtaaagac caggctcgcc agtaacgcta aatccatttg gctgtaagcg
     1321 cggtgtcatc cgcggcagga aaattaaaca gttactttaa aaaatgaaaa cgtaaaaagg
     1381 ttgggtttcg atgtattgac gggtaaactt tgtcgcccgc taaacatttg tttaaggaat
     1441 gattaattgt gaccaaactc aaacttctgg cacttggagt gcttatcgca acgtctgcag
     1501 gcgtagcgca cgctgaaggt aaattttccc tgggcgcagg cgtaggtgtc gttgagcacc
     1561 catataaaga ttacgatacc gatgtttacc cagtaccggt aatcaactat gaaggcgata
     1621 acttctggtt ccgtggctta ggtggtggtt actacctgtg gaatgacgca acggataaac
     1681 tttcaattac cgcttactgg tcgccgcttt acttcaaagc gaaagacagt ggcgatcacc
     1741 aaatgcgtca cctggatgac cgtaagagca ccatgatggc tggtctgtct tatgctcact
     1801 ttacccagta cggttacctg cgtaccaccc tggctggcga taccctggat aacagcaacg
     1861 gcattgtctg ggatatggcc tggttgtatc gttacaccaa cggtggcctg accgtgactc
     1921 cgggtattgg tgtgcagtgg aacagcgaaa accagaacga atactattat ggcgtatcgc
     1981 gcaaagagtc cgctcgcagc ggtctgcgtg gctataaccc gaatgacagc tggagccctt
     2041 accttgagct gagcgccagc tacaacttcc tcggcgactg gagtgtttac ggtaccgcgc
     2101 gctacacccg tctgtctgat gaagttactg acagcccgat ggtggataaa tcctggactg
     2161 gcctgatttc taccgggatc acctacaaat tctgataatg catctttcca gggcattttg
     2221 tgcataaaaa cagggcgtca cgccctgttt tgcattacgg tggtgcaata cctgggagga
     2281 aacaatgcaa caaaaaatga ttcaatttag tggcgatgtc tcactgccag ccatagggca
     2341 gggaacatgg tatatgggcg aagatgccag tcagcgcaaa acagaagttg ctgcactacg
     2401 cgcaggcatt gaactcggtt taaccctcat tgataccgcc gaaatgtatg ccgatggcgg
     2461 tgccgaaaag gtggttgggg aagcattaac cggtctgcga gagaaggtct ttctcgtctc
     2521 taaagtctat ccgtggaatg ctggcgggca aaaagcgata aatgcatgcg aagccagttt
     2581 acgccgtctc aatactgact atctcgatct ctacttatta cactggtctg gcagtttcgc
     2641 ttttgaagag actgttgccg cgatggaaaa attgatcgcc cagggaaaaa tccgtcgctg
     2701 gggcgtttct aaccttggtt atgccgatat gcaggaactc tggcagctgc cggggggaaa
     2761 tcagtgtgcc actaatcagg tgctttacca tctcggttca cgagggattg agtacgatct
     2821 actcccctgg tgccagcaac agcagatgcc ggtgatggct tacagtccat tagcccaggc
     2881 cgggcggttg cgcaatggac tgttaaaaaa cgcggtagtc aacgaaattg cacatgtcca
     2941 acaacatgca gctgcgcttg aggtcgaact ttcgtcagcg gaattagcta tgctggataa
     3001 ggcatatccg gcaccaaaag gaaaaactgc gctggatatg gtgtgatgtt gatacgtgcc
     3061 ggatgcatcg cacccggcac acaacattaa cgcaaattaa cgtttggcaa cgcgaatgga
     3121 ttgcgccaga tgtgcaggtt tctctttggt gactttttgc gtttctgaag cgtaagccgt
     3181 ttctacacaa acaaaggtct tgtagccatc gtccggcatg tcgcccatgc taactgaaag
     3241 cgccggtccc gggttccagc cgacaacgtt cagatgatgc tggtggccta cggcgataat
     3301 acgattcagc gcttcatcat taatcacgct gcaatcttgc ggattcagat acacgcgatc
     3361 ggtacggtca gggaaggtct gaataccgtc ggtcagtaca tcttctttcg cgtcattcac
     3421 tttatcaatg aagcgatcgc ccagcccact gacgcttacc ttagcgatat cacccacgtt
     3481 aaagtaggta tgcagggcag aggtggtttc aaattcgcca tgtgattcaa gatcgatttc
     3541 acaggtttta cccacgcgga aatgcgctaa cagcgtaaag tcgtgcggcc agaatttttt
     3601 cgtctcttcg ctttgtgtca attcaaaagt cagcgctacg ccatcagcat cttcatgatg
     3661 tgatttcagc gtccacggca ggttgcgggc aaaaccgtgc gcaggcagac cttgttgtgc
     3721 cgccggacca aaccacggcc agcaaaccgg tacgccaccg cgaatagcga cgccattttt
     3781 gaacggtgtg ttgttgctca accacagaac ttcttcttca cccgcaggtt tccacgagag
     3841 aaggtgtgcg ccctgtaatg caaaagaggc ttttacctgg ggatgatcga ccacaatgag
     3901 gtccagttca tccagtttac gacgggagag gacaggggag atttgttcga tgaccggaag
     3961 ggcaaaaatt ttcttaatca tgacgcagtc ctttaacttc attttatcag gtaaaaaaaa
     4021 gagcgaccga agtcgctctt tttagatcac agtgtcatct caacttattt ggagatgtga
     4081 gcgatcaggt ccagaacttt gttggagtaa ccggtttcgt tgtcgtacca ggataccagt
     4141 ttcacgaagt tgtcgttcag agcgatacca gctttagcat cgaacacgga agtgcaaact
     4201 tcgccgttga aatcggtaga tactacgtca tcttcggtgt agcccagaac gcctttcatt
     4261 tcgccttcag cagcagcttt aacggcagct ttgatctgct cgtaagttgc agctttttcc
     4321 agacgaacgg tcaggtcaac tacggatacg ttcggggtcg gaacgcggaa cgccatacca
     4381 gtcagtttgc cattcagttc tggcagtact ttacctacag ctttagcagc accggtagag
     4441 gacgggatga tgttctggga agcgccgcgg ccgccgcgcc agtctttgtg agacgggcca
     4501 tcaacggttt tctgagtagc ggtagtagcg tgaacggtgg tcatcagacc ttcgatgatg
     4561 ccgaagttat cgttgataac tttagccagc ggagctaggc agttggtggt gcaggaagcg
     4621 ttggaaacga tgtcctggcc agcatatttg tcgaagttag cgcctttaac gaacatcgga
     4681 gtgttgtctt tagacggacc agtcataacc actttcttcg caccagcggt gatgtgttta
     4741 cgagcagttt cgtcagtcag gaacagacca gttgcttcag cgacaacgtc aacacccact
     4801 tcgtcccatt tcaggttagc cggatcacgt tcagcggtaa cacggatttt tttaccgtta
     4861 acgatcagat gaccgtcttt cacttcaacg gtaccgtcga aacggccgtg agtggagtca
     4921 tatttcagca tgtatgccat gtaatcagcg tctaacaggt cgttgattgc aacgatctcg
     4981 atgtcagaac gtttctgagc agcacggaaa acaatgcgac cgatacggcc aaaaccgttg
     5041 atacctactt tgatagtcat atattccacc agctatttgt tagtgaataa aaggttgcct
     5101 gtaaaattac aaaaacctta cgcagcgtca agcggaatcg tgtcaatcat tgcgacaaat
     5161 caatcctgtg cctaagcatt acgcgactga ctcgcctcac ttttcctttg ggcttgagac
     5221 cacatggggt cggcgccccg aattttaaag ggcaattacg ataaaaatgt gattttcatc
     5281 acgatttagg tgcagccatt tcgtcgagat caagtttagg acaaaatttt gccagttggt
     5341 gttaatgttt tgttagaatc agtcacgtaa tgtgagcacg attaaagtga gatgtgagta
     5401 aatggcgaat aaaccttcgg cagaagaact gaaaaaaaat ttgtccgaga tgcagtttta
     5461 cgtgacgcag aatcatggga cagaaccgcc atttacgggt cgtttactgc ataacaagcg
     5521 tgacggcgta tatcactgtt tgatctgcga tgccccgctg tttcattccg aaaccaagta
     5581 tgattccggc tgtggctggc ccagtttcta cgaaccggtg agtgaagaat ccattcgtta
     5641 tatcaaagac ttgtcacatg gaatgcagcg catagaaatt cgttgcggta actgtgatgc
     5701 ccatctgggg catgtctttc ccgacgggcc gcaaccaaca ggcgaacgtt attgtgttaa
     5761 ctctgcttct ttacgcttca ctgatggcga aaacggcgaa gaaatcaacg gttgaaaaaa
     5821 cgattcagca acttattcca caggagcgga aaaattatga atcttgatga cattatcaac
     5881 agcatgacgc ctgaggtata ccagcgtttg tcgaccgccg ttgaactggg gaaatggcct
     5941 gatggcgttg cgttgaccga ggaacaaaaa gagaattgcc tgcaactggt gatgctgtgg
     6001 caagcccgcc ataataccga agcacagcat atgacaattg acacaaatgg tcagatggtc
     6061 atgaagagca aacagcagtt aaaagaagat tttggtatct cagcaaagcc tattgccatg
     6121 tttaagtaag tggttcatcc cctttttgcc gcggctatga aggggatagc caacaatgac
     6181 cccacgactc gtctcaatta tctcgacccg acctggctaa gtcccagtcc gatctcttgc
     6241 agtcgcgttc acgcctgaat gacttctcct gaacttaagt ctcagaccta tttggccggt
     6301 aatccctctc gaatttgctc tttttgcgtc ccgccttcca ccaacctgta acagacccag
     6361 ccattttctg cgtaattgtg atctgagttg taaaatgcag gtcattatct gtgttgtgca
     6421 gatttaatat tgacctcaaa aatcatgtgg gtaagtatga tggccatcta agcaaaacaa
     6481 aacacagagg atgacgcaat gaaagcactg gctcggtttg gcaaggcctt tggcggctac
     6541 aagatgattc atgtcccaca acccatttgt ggcccggaag acgtagtgat tgaaattaaa
     6601 gccgcggcaa tctgcggcgc agacatgaag cactacaatg tcgatagcgg ttctgatgag
     6661 tttaactcta tccgcggcca tgagttcgca ggttgtattg cgcaggttgg tgaaaaagtc
     6721 aaagactgga aagtggggca acgcgtcgtg tcggataaca gcggtcacgt ttgcggcgtt
     6781 tgtccggcct gtgaacaggg tgattttctg tgttgtacag aaaaagtaaa ccttggtctg
     6841 gataacaata cctggggcgg tggtttttcc aaatattgtc tggttcctgg tgaaattctc
     6901 aaaattcatc gtcatgcgtt gtgggaaatc cctgatggtg ttgattatga ggacgcagcc
     6961 gtacttgacc ctatctgtaa tgcctataaa tccatcgcgc agcaatcgaa attccttcct
     7021 ggtcaggatg ttgtcgtcat tggcactggc ccactcgggc tgttttccgt acaaatggcg
     7081 cggattatgg gggcggtaaa tatcgtcgtc gttggtctgc aagaagatgt ggcggtccgc
     7141 ttcccggttg caaaagaact gggggcgacg gcagtagtaa atggttctac cgaagatatg
     7201 gtggcgcgct gccagcaaat ttgtggcaaa gataatctgg gactggtgat tgaatgctcc
     7261 ggtgccaata tcgcactgaa acaagccatc gaaatgcttc gcccgaatgg ggaagtggta
     7321 cgcgttggaa tgggcttcaa acctcttgat ttctcgatta atgacattac cgcctggaat
     7381 aaaagcatca ttgggcatat ggcctatgac tccacctcat ggcgtaacgc tatccggcta
     7441 ttagccagcg gcgctatcaa agtcaaaccg atgatcacgc atcgtatcgg cctgtcgcaa
     7501 tggcgcgaag ggtttgatgc gatggtcgat aaaaccgcaa tcaaagtgat catgacttac
     7561 gactttgatg aataactact ctcctgaagg aacgttatcg catggaacag ataacaaaac
     7621 cgcattgtgg tgcacggctg gatcgcttac cggattgccg ctggcattcg tcaatgtttg
     7681 ccatcgtcgc gtttggcttg cttgtctgct ggagtaatgc cgttggtggc ttgatcctcg
     7741 cgcagctgaa agcgttgggc tggacagata attccactac agccacattc tcagcaatca
     7801 cgaccgccgg aatgtttctc ggtgcgctgg tgggcggcat cattggcgac aaaaccggtc
     7861 gcagaaatgc gttcatcctc tatgaggcca ttcatattgc ctcgatggtt gtcggtgctt
     7921 tctcaccgaa tatggatttt cttattgcct gccgttttgt gatggacgtt gggctgggag
     7981 ctttactggt tacactgttt gctggtttca ccgaatatat gcccggtaga aatcgcggaa
     8041 cgtggtccag tcgggtttcc tttattggca actggtcata tccgctctgc tcactgattg
     8101 cgatgggact cacgccgctg attagtgcag agtggaactg gcgggtacaa ctgcttattc
     8161 ctgcaatatt gtcgcttatc gctacggcgc tggcctggcg ctactttcct gaatccccgc
     8221 gctggctgga atcgcgcgga cggtatcagg aagccgagaa agtcatgcgg agtatagaag
     8281 aaggcgtcat acgccagacg ggtaaacctt tgccgcctgt ggttattgct gatgacggta
     8341 aagcgccaca agcggtgccg tattcagcct tactgacagg agtattactg aaacgcgtga
     8401 tattaggctc ttgtgtgctg attaccatga acgttgtgca gtacacacta attaactggt
     8461 tgccaacaat attcatgacc caggggatta atttaaaaga ctcgattgtt ttaaatacca
     8521 tgagtatgtt tggcgcgcca tttggtattt ttattgccat gctggtgatg gataaaattc
     8581 cgcgtaaaac aatgggtgtg gggctattaa tcctgattgc ggtgctcgga tatatctatt
     8641 cactgcaaac cagtatgttg ctcataacgc tgattggttt cttcctgatt actttcgtct
     8701 atatgtacgt ttgctatgcc tcggcagtgt atgtccctga aatctggccg acagaggcca
     8761 aactccgtgg ctccggtctg gcgaatgcgg tagggcgaat cagtggtatt gccgcacctt
     8821 atgccgttgc agtgctgctc agtagttatg gtgtaacggg agtctttatt cttctggggg
     8881 ctgtttcaaa tattgtcgca attgctatcg ccaccattgg aattgaaacc aaaggtgtct
     8941 ccgttgaaag tttaagtatt gatgcagtag ccaataaata atacgggtgg attcaaaatg
     9001 aaaaattcaa aagcaatatt gcaggtgccg ggcacaatga aaattatttc agcagaaata
     9061 ccagtgccta aagaagatga agttttgatt aaagtagaat atgtcggtat ttgtggttca
     9121 gatgtacatg gttttgaatc aggcccgttt attccgccta aagacccaaa tcaagaaatt
     9181 ggcctgggtc atgaatgcgc cgggacggtt gtggctgtgg gaagccgcgt gcgcaaattt
     9241 aaaccggggg atcgggtaaa tatcgaacct ggcgttcctt gcggtcactg tcgttactgt
     9301 ctggaaggca aatataacat ctgcccggac gttgatttta tggcgacaca acccaactac
     9361 cgcggcgcat taacgcacta tctgtgtcat ccggagagct ttacttacaa actgcccgac
     9421 aatatggaca cgatggaagg ggcgctggtg gagcctgccg cagtcgggat gcatgccgcg
     9481 atgctggcag atgttaaacc gggtaagaag ataattattc tgggagcagg ttgtattggt
     9541 ttgatgacgt tgcaagcgtg caaatgcctg ggcgcaacgg aaattgccgt cgttgatgta
     9601 ctggaaaaac gtctggcaat gggggaacag cctggtgcga cagtggttat taatggcgca
     9661 aaagaggaca ctattgcacg ctgtcagcaa tttaccgaag acatgggcgc agatattgtt
     9721 ttcgaaacag cgggttctgc ggtcaccgtt aaacaggcac ctttaatctg gtaatgcgcg
     9781 gcggtaaaat tatgattgtt ggtactgtac ccggcgattc ggcaatcaat ttcctcaaaa
     9841 tcaatcgcga agtcactatc cagacggtat tccgctatgc caatcgttat ccggtcacga
     9901 ttgaagctat ttcttcaggg cgattcgatg tgaaatcgat ggtgacgcat atttacgatt
     9961 atcgggatgt acaacaggca tttgaagagt cagttaacaa caaacgcgac attattaaag
    10021 gcgttattaa aattagcgat taattaatac gggagaacag aacatgctcg cagatatcag
    10081 atattgggaa aacgatgcca caaataaata ttatgcaatt gcccatttta acgtatggaa
    10141 tgcagaaatg ttgatgggcg ttaaagacgc agccgaagaa gcgaaatccc cggttattat
    10201 ttcttttagt acaggttttg tcggtaacac ctcatttgaa gatttctctc acatgatggt
    10261 atcaatggca caaaaagcaa cggtgccggt aataactcat tgggatcatg gtcggagtat
    10321 tgagattatt cataacgcct ggactcatgg tatgaattca ttaatgcgtg atgcttctgc
    10381 atttgatttc gaagaaaata ttcgtttaac caaagaggct gttgatttct ttcatccgct
    10441 gggtattccg gtagaggcgg aattagggca tgtcggtaat gaaaccgttt atgaggaagc
    10501 gctggcgggc tatcactata ccgatcctga ccaggcggct gaatttgttg aacgtacggg
    10561 ctgtgactca ttggctgtcg ccatcggcaa ccagcatggg gtttatacgt cagagccgca
    10621 attgaacttt gaggtcgtca aacgcgtacg cgatgctgtt tctgttccgc tggttttgca
    10681 tggcgcatcg gggatcagtg atgccgacat taaaactgca atttcgttgg gtattgcgaa
    10741 aatcaacatt catacggagc tctgtcaggc cgcaatggta gcagtgaaag aaaaccagga
    10801 tcagcctttc ctgcatttag aacgtgaagt acgtaaagct gtaaaagaac gggcattgga
    10861 aaaaattgaa ctgttcggtt ctgatggcaa agcggaataa tgatatggat aatctcgacg
    10921 ttatttgtat aggtgccgct attgttgata ttccattaca accggtcagt aaaaatatct
    10981 ttgatgtaga ttcttatcct cttgaaagaa tcgcaatgag caccgggggc gatgcaataa
    11041 atgaagcaac aattatttct cgtctggggc atcgtaccgc attaatgagc cgcattggta
    11101 aagatgcctc agggcaattt attctcgacc attgtcgcaa agagaatatt gatattcaaa
    11161 gcctgaaaca ggacgtcagc atagatacat ctattaacgt tggactggtg acggaggatg
    11221 gcgagcggac gtttgtcacg aaccgtaacg gcagtttgtg gaaattaaat attgacgatg
    11281 ttgattttgc gcggtttcct caggcgaaat tattatcact ggccagtatt ttcaacagtc
    11341 ctctattgga tggtaaagcg ctaacagaaa tttttacgca agcgaaagcc cggcagatga
    11401 tcatttgtgc cgatatgatc aaaccgcggt tgaatgaaac gctggatgat atttgcgaag
    11461 cattgagtta tgtcgattat ctgtttccaa attttgccga ggaaaaatta ctcacaggga
    11521 aagagacact ggatgaaatt gctgactgct ttcttgcgtg cggcgtaaaa acggtggtga
    11581 ttaaaacggg taaagacggc tgctttatca agcgtggtga catgacgatg aaggtgccgg
    11641 cggtcgcagg aataaccgcc atcgacacca ttggcgcggg cgataacttt gcttcaggtt
    11701 ttattgcggc actgttagaa ggcaaaaatc tgcgtgaatg cgcacgcttt gccaatgcaa
    11761 cggcggctat ctcggttcta agcgtcggtg ccaccaccgg cgtaaaaaac agaaagctgg
    11821 tcgaacaatt gctggaagaa tacgaaggat aatgaaggca aatgaaaaag atacctttag
    11881 gcacgacgga tataacgctt tcgcgaatgg ggttggggac atgggccatt ggcggcggtc
    11941 ctgcatggaa tggcgatctc gatcggcaaa tatgtattga tacgattctt gaagcccatc
    12001 gttgtggcat taatctgatt gatactgcgc taggatataa ctttggcaat agtgaagtta
    12061 tcgtcggtca ggcgttaaaa aaactgcccc gtgaacaggt tgtagtagaa accaaatgcg
    12121 gcattgtctg ggaacgaaaa ggaagtttat tcaacaaagt tggcgatcgg cagttgtata
    12181 aaaacctttc cccggaatct atccgcgaag aggtagaagc cagcttgcaa cgtctgggta
    12241 ttgattacat cgatatctat atgacgcact ggcagtcggt gccgccattt tttacgccga
    12301 tagctgaaac tgtcgcagtg cttaatgagt taaaggctga agggaaaatt cgcgctatag
    12361 gcgctgctaa cgtcgatgct gaccatatcc gcgagtatct gcaatatggt gaactggata
    12421 ttattcaggc gaaatacagt attctcgacc gggcaatgga aaacgaactg ctgccgctat
    12481 gtcgtgataa tggcattgtg gttcaggttt attccccgct agagcaggga ttgttgaccg
    12541 gcaccatcac tcgtgattac gttccgggcg gcgctcgggc aaataaagtc tggttccagc
    12601 gtgaaaacat gctgaaagtg attgatatgc ttgaacagtg gcagccactt tgtgctcgtt
    12661 atcagtgcac aattcccact ctggcactgg cgtggatatt aaaacagagt gatttaatct
    12721 ccattcttag tggggctact gcaccggaac aggtacgcga aaatgtcgcg gcactgaata
    12781 tcaacttatc ggatgcagac gcaacattga tgagggaaat ggcagaggcc ctggagcgtt
    12841 aaatactcaa cacgattcac ctctgtttct gtgaaaactg tgatgaaaat ctgtgattca
    12901 aataggttat tttgaaagta aacatcggtt atgcgataat cgcgctaatg tgatgtaaaa
    12961 agatacaggg gtgtaatcgt ggcggcaaaa gacaggattc aggcaattaa gcaaatggtt
    13021 gccaacgata aaaaggtgac agtctcaaat ttgagtggga tttttcaggt aaccgaagaa
    13081 accattcgcc gcgatcttga gaagctggaa gatgaaggct ttttgaccag aacctatggt
    13141 ggtgctgttt taaatacagc ggtgttgacg gagaatatcc atttttataa gcgcgcttca
    13201 tcgttttatg aagagaagct gcttattgca cgcaaggcac taccctttat cgacaataaa
    13261 accaccatgg cagccgattc cagtagtacc gtaatggaat tgctcaaatt gttacaggac
    13321 cgtagtgacc tgacgttgct aaccaactcg gcggaagcga ttcatgttct ggctcagtca
    13381 gaaattaaag tcgtttcaac gggtggggaa ctaaacaaaa atacactttc cctgcaagga
    13441 agaattacta aagagatcat cagccgctac catgtcgata tcatggtaat gagttgtaaa
    13501 ggtcttgata ttaacagcgg cgcgctggac tctaatgaag cggaagctga aatcaaaaag
    13561 acaatgatcc gtcaggcgac agaagttgcg ttattggttg atcactctaa gtttgatcgt
    13621 aaagcttttg tccagttagc tgactttagt catattaatt atataataac tgataaatca
    13681 ccgggtgcag agtggatagc attttgcaaa gacaataata tccagctggt gtggtaattt
    13741 aagcacacgt tgtcaatatt atttgttcct gcgactggaa ataaaatgat gataaagatg
    13801 cggcaagtga atttgccgca attgctacct tttatagcaa atccgaggca cccaatggaa
    13861 caatatgatc aaattggcgc aagactggac cgcttgcctt tggcccggtt tcattatcgt
    13921 atatttggta taataagctt tagtctgtta ttaacggggt ttttgagtta ctcaggtaat
    13981 gtcgtcttag caaagctggt aagcaatgga tggtcaaata atttcctcta tgccgccttt
    14041 acctcggcat taatgtttgg ttatttcatc ggctcactta ctggtgggtt tattggtgac
    14101 tactttgggc ggcgcagggc gtttcgcata aatcttctca tcgtcggtat tgctgcaaca
    14161 ggggccgctt ttgtccctga tatgtactgg ctcattttct ttcgcttcct gatgggaaca
    14221 ggaatggggg cgctgattat ggttggctat gcctcattta cggagtttat ccccgcgacg
    14281 gtgcgtggaa aatggtccgc gcggctctca tttgttggta actggtcgcc catgctgtct
    14341 gcggcgatag gcgtggtggt tatcgctttt tttagttggc gaataatgtt tcttctgggt
    14401 ggtattggca tactgttagc ctggtttctc tcaggtaaat actttatcga gtcgccacga
    14461 tggctggaag ggaaagggca aatcgccggt gcagaaagcc aacttcgtga agtagagcag
    14521 caaattgaaa gggagaagag tattcgttta ccccagctta ctttgaacca gagcaacagc
    14581 aaggttaagg taatcaaggg taccttcagg ctcctgttta aaggggaaat gttacgacgt
    14641 acattagttg cgataactgt tttaattgca atgaacattt cgctttatac cattaccgta
    14701 tggataccga ccatatttgt taactccggc attgatgtcg ataaatcaat attaatgacc
    14761 gctgttatta tgattggtgc tccggtagga atatttattg cagcattaat tattgatcat
    14821 tttcctcgtc gattatttgg ctccgcctta cttattatta ttgccgtgtt aggctatatc
    14881 tattcaagtc agactacaga gtgggcgatt ttaatctatg gtctggtgat gatcttcttt
    14941 ttatacatgt atgtttgctt cgcgtcggcg gtttatatcc cggagctttg gccaacgcat
    15001 ttacgcctgc gcggttcggg tttcgttaat gccgtcggac ggatcgtcgc agttttcacg
    15061 ccctatggcg ttgcggcatt attaacacat tatgggtcga tcacggtgtt tatggtgctt
    15121 ggtgtcatgt tagtgctctg tgcgctggtt ctctccattt ttggcatcga aacgcggaag
    15181 gtgtcgctgg aagagatttc tgaggtgaat taaatctttc gttgatggca tcaattgcca
    15241 gatgtaccac tgacgtgttt tatttggcct acaatgccag tacagaattg taggccagcg
    15301 taaaattacc cctgcgtctc ttcccagtct gccagcgtat atagcgttgc cccagctgct
    15361 gacatctcca taaacgcgtg tgcactgtcc tggggctgga tattcacgcc acgacagcca
    15421 tcggtaatca cgtttacctt ataacctaac tgtaacgcgt ccagcacggt aaacttcacg
    15481 caatagtcag tagccaggcc cataacgatc aattcatcga tttcatgatc gcgcaaccag
    15541 tcatcaagcg cggttttctg ccgacgtccg ttatcaaaaa aggcactgta actgtcaact
    15601 aaaggatttt cgcctttatg gaacaccgct gcgatctctt tttgattcag taacggatgt
    15661 aattgtgcgc cttcactgtt ctgcacacag tgttcaggcc agaaagtttg ccgcaggccg
    15721 ccgagttggc ctggcgtata aggctctacg ccgtgctggc tggcaaagct gccgtgattc
    15781 gccggatgcc agtcctggct ggcgataacc gcttctccgc gcgactggca ccagtcaata
    15841 aggcggttag caacctccac cgtactgtca ccttccggta cggcgagcgc gccaccagca
    15901 cagaaatcat tttgtaaatc gaccagtaac agggcgcgat ggggcattgc agtctcctta
    15961 atcatccggc gtcagttcgc cgcgcaggtt ttggctcatg gccttgcgaa tggtttcagt
    16021 atccagttcc tggctcagca ggtaatgcag tttggttagc gtggcttcta cagtcatatc
    16081 tgcaccgtca ataacgccgg catgggcgag ggcgttaccg gtggcataac cactcatatt
    16141 cactttaccg gacatacatt gtgtcaggtt gaccaccaca ataccgcgat cgctggcttc
    16201 ttgtaattcc tgcaggaagg ctttgttttg tggcgcatta cccacgccat aggagcgcag
    16261 aatcaatgct ttcaccggtt ggcgcagaaa attgcgcacg acgtcagcag aaatccctgg
    16321 ataaatcgtc accacgccaa ttggttgtgg cgtgattgga tgaacgatta attccccttc
    16381 accgtgcggg gcgggtggcg tattcaaacg acgaatatgg ataccagctt ccagtaacgg
    16441 aggaaggttt ggagaggcaa acgcatcaaa accatcggca tgggctttgg tagtgcggtt
    16501 gccgcgatac aatcggttat tgaaaaagag cgttacttcg ttgatcggat aattcgccgc
    16561 aacgtacaac gcattcagca gattaatttg tccgtcagag cgtaactcag ccagcgggat
    16621 ttgtgaccct gtcacaatga ccggtttacc gagattctcg agcatgaacg acagcgcaga
    16681 ggcggtatac gccatcgtgt cggtaccgtg tagaatgaca aaaccatcat agtcgtcata
    16741 gtgcgcttta atatcttcag caatatgctg ccagtcttcc ggcgtcatat cagaagaatc
    16801 catcaacggc gtatattcat gaatggtgaa atctggcatc tccgggcgat ggaattccgg
    16861 catcagcgcc agttggcgtt gtagatgacc tgacaccgga atataaccct gctcggaacg
    16921 ctgcatcccg atggtcccgc ccgtgtaggc gacgtaaatt gatttctttt gcatgatatt
    16981 gatatgaatg atatcgaaag agcaaaagta tatacgtgag gcaggaggag cgcacctgat
    17041 actatgcgga taaggaaata tggggcgaga cagcgggaag aggattcgtc gaggtaaaaa
    17101 ataatgtggc cgacgatctg tcggccactc agtacaagac ttaacgcacg ttggcgcagg
    17161 tcaggcaaaa cgcataacgg ttttgtgggt cgttaaacgc ggccagcttg tcgctttcac
    17221 ttttaacggt agaggctacc gaggcaagcg gtgcaggtaa catggcctgg aacgcatctg
    17281 gcaacattgc ccggacagaa ccagacatgt tgtccatcac tttgtcgaag aaggtcggtt
    17341 catcaacgta gtattccaga tgccactgtt tcacttttgc gagctctgct gctttggcaa
    17401 ccgcatcatc gaaatccccg agactatcga ccagcccgtt agcttttgca tcctgaccgg
    17461 tccagacgtg gccctgggcg attttatcaa tttgctccgg cgtcgaatga cgcgcatcag
    17521 caaccagcgt gataaagcgt ttatagccat tctcaatgct taattgcatc atctgctgcg
    17581 cttccggcgg cagtgccctg gtgatagaaa catccgccag cggtgaagtt gagacaccat
    17641 cagtatgaac accaatcgaa tccagactat tttctacggt ggtgatcacg ccgaagatac
    17701 cgatagaacc ggtcagggtg ctggggttag ccacaatgta attagctggc gtggaaatcc
    17761 agtaaccacc agatgccgcc atgccgccca tcgatacaac cacagactta cccgctgccc
    17821 gggctgctgc cagttcagcg cgaatcactt cagacgcggt aacgctgccg cctgggctat
    17881 taacacgcag gacaatcgct ttcactttcg ggtcaaggcg agcgtcgcgg atttgtgccg
    17941 cagtggtatc accgccaaca ttcccctgag tttcctcgcc atccataatt gcgccattag
    18001 cgaagacgac accgatgctg tcaccggtat ctgccggcgt tttcaatgcg taatcgtaat
    18061 aactgatggc gcgataattt ttatcagtct tactccagcc gaactctttg gtcagagctt
    18121 tttcgatttc ggcactcgat gccagtgcat cgaccagctt gttttccagt gcatatttcg
    18181 cggtatcgcc accggttttg gttaaaccct caagcaaccc ttgcgcgcca gggaatacct
    18241 gctgagcagg gatctgccgg ttagcggcaa cagtattcag atagttttgc cacagctcac
    18301 caatccagcg gctgtcagct tcgcgggctg ccggtgacat atcatcacga ataaacggtt
    18361 caacggcaga tttatacgta cccacgcgga acacatgggt ggaaactttc agcttatcca
    18421 gcaacgattt gtagtacaga ccgttggtag caaaaccgtg cagatcaacc acgccttgcg
    18481 gtgacagcca aattttattg gcgaaactgg cgagataata ttgcccctgg ctgtagttct
    18541 cgccaacggc ataaaccggt ttcccgctgt cacgaaactc tttcagggct ttgccgatgt
    18601 actgcataga cggctggtcg ccgcctgcga agtttttcag atccatcacg ataccggtga
    18661 tattgcggtc gtccttcgcc tggcgaatgg tgttgacgat atcaaacagt gagttttcct
    18721 gcagacgatc ggaactggca ccaagcagct ggcggcttaa tttactaaac cgctgagaac
    18781 tgtctggttt atcgacgatc acaccagaaa tgtccagcag cagtgcgcca cgactggccg
    18841 tttctttcga atcaccacca ctgacctgca tccagatccc cacaccaacc agtacgagga
    18901 aaataaagaa caggttaagt accatttcac ggacgaaatt cagcagacgc cacgtccatt
    18961 taaaaaatcc ggcaataaat cgccaaaggg ttcgcatgta ttctcccaac ttaaggtcac
    19021 acctgtcaca ggcgcaatag atgtggttat cgtaatgacc caaccggcac ttgtcagcag
    19081 gaattgcgga ctgtgctgta acaaaatcca ctctcgtgtt aattttgtga ataaatatca
    19141 cgacaggagt taatcaaatg gatgcactcg aactattgat caatcgccgt agcgcctccc
    19201 gcctggctga acccgcgcca acgggtgaac aactgcaaaa catcctgcgt gcgggtatgc
    19261 gtgcgccgga ccataagtcc atgcaaccgt ggcatttttt tgtgattgaa ggggaagggt
    19321 gcgagcgttt cagcgccgta ctggaacagg gggcgattgc tgccggtagt gatgataaag
    19381 ctatcgacaa agcccgtaat gcgccgttcc gcgcaccgct catcatcacg gtggtggcga
    19441 aatgcaaaga aaatcataaa gtcccgcgat gggaacagga aatgtctgcc ggatgcgcgg
    19501 tcatggcgat gcaaatggca gccgttgccc agggatttgg cggcatctgg cgcagtggcg
    19561 cattaactga aagtccggta gtgcgtgaag cattcggttg ccgtgagcag gataaaattg
    19621 tcggttttct ctacctcggc acgccgcagt tgaaagcatc tacgtcgatt aacgtcccgg
    19681 acccgacgcc gtttgtaact tatttctgat aattgtcgcg aaactgtctg gattgtgagc
    19741 aaaggcgcag gaattcagac actctcactt atcacttcac ggaatgaggg ttaccatacc
    19801 cggattgcat tgacaggaga tgtccatgag cgagaactcg attcgtttga cccaatacag
    19861 ccacggagct ggttgcggct gtaaaatttc cccaaaagtg ttggaaacca tcctgcacag
    19921 tgagcaggcg aagtttgttg atctgaattt gcttgtgggt aatgaaaccc gcgacgatgc
    19981 ggcggtgtac gatctgggca atggcaccag cgttatcagt accaccgact tctttatgcc
    20041 gatcgttgat aatcctttcg attttggccg cattgcggcg actaacgcca tcagcgatat
    20101 cttcgcgatg gggggcaaac cgattatggc gattgcgatc ctcggctggc cgattaacaa
    20161 actttccccg gaaattgccc gcgaagtaac cgaaggtgga cgctatgcat gccgtcaggc
    20221 gggtattgcg ctggctggcg gtcactccat cgatgcgccg gagccgattt ttggtctggc
    20281 ggtaacgggg atcgtaccga ccgagcgggt gaagaaaaac agtaccgcac aagccggatg
    20341 caaactgttc ctgacgaaac cgctggggat cggcgttctt accacggctg agaaaaaatc
    20401 actgttgaaa ccagaacatc agggactggc gacggaagtg atgtgccgga tgaacatcgc
    20461 aggcgcgtcc tttgccaaca tcgaaggcgt aaaagcgatg accgacgtta cgggctttgg
    20521 tctgctgggc cacttgagcg aaatgtgtca gggggctggt gtgcaggcac gcgtcgacta
    20581 tgaagcgatc ccgaaactcc ccggtgttga agagtacatt aagttgggcg cagtacctgg
    20641 cggcactgaa cgtaactttg ccagctacgg tcatctgatg ggtgaaatgc cgcgtgaagt
    20701 gcgcgatctg ctgtgcgatc cgcaaacttc tggcggtttg ctgctggcgg tcatgccgga
    20761 agcagaaaat gaggttaaaa ctacagccgc cgagtttggc attgaactga cggcgattgg
    20821 cgaactggtg ccagcgcgcg gcggtcgtgc catggttgag atccgttaat ttaatgcggt
    20881 tgtttattgc cgaaaaaccg agtctggcgc gcgccattgc tgatgtcctg cccaaaccgc
    20941 accggaaagg cgatggcttt atcgagtgcg gtaatggtca ggtggtgact tggtgtatcg
    21001 gtcacctgct tgagcaggcg cagccagacg cctacgacag ccgctatgcg cgctggaatc
    21061 ttgcggattt gccgattgtc ccggaaaagt ggcaattaca gccacgaccc tccgtgacca
    21121 aacaacttaa cgtcatcaaa cgcttcctgc atgaagccag cgaaatcgtt cacgccgggg
    21181 acccggatcg tgaagggcaa ttgctggtgg atgaagtgct ggactatctg caactggcac
    21241 cggaaaagcg ccagcaggtg cagcgttgct tgataaacga cctgaacccg caggcggttg
    21301 agcgggcgat cgaccgtctt cgttccaaca gtgagtttgt gccattgtgc gtttctgcgc
    21361 tggcgcgagc gcgtgccgac tggctgtacg gcatcaatat gacccgtgcg tataccattc
    21421 tcggtcgcaa tgccggttat cagggcgtac tttccgtggg acgcgtgcag acacccgtgc
    21481 tcgggctggt ggtgcgccgc gatgaagaga ttgaaaactt cgtggcgaaa gacttctttg
    21541 aagtcaaagc acatatcgtg acacctgccg atgagcggtt taccgctatc tggcaaccga
    21601 gcgaagcgtg tgaaccgtac caggatgaag aagggcgctt gttacatcgt ccactggcgg
    21661 agcatgtggt taaccgcatt agtggtcaac cggctattgt caccagctat aacgataaac
    21721 gggaatcaga atccgcgccg ctgccgtttt cgctttcggc gttgcagatt gaagcggcaa
    21781 aacgcttcgg tctgagcgcg cagaacgtgc ttgatatctg ccagaagctg tacgaaacgc
    21841 acaagctaat cacttatccg cgttccgatt gtcgctattt gccagaagaa cattttgccg
    21901 gacgccacgc ggtgatgaat gccatcagcg ttcatgcacc ggatctgttg ccgcagccag
    21961 tggtagatcc agatatacgc aaccgctgtt gggatgacaa aaaggtcgat gcgcaccacg
    22021 ccatcatccc gaccgcacgg agttctgcga tcaacctgac ggagaacgaa gcgaaggtct
    22081 ataacctgat tgcccgtcaa tacctgatgc agttctgccc ggatgcggtg ttccgcaagt
    22141 gtgttatcga actggacatt gccaaaggca aatttgtcgc taaagcccgt tttcttgctg
    22201 aagcaggctg gcgcacgctg ttaggcagca aagagcgcga tgaagaaaac gacggcacgc
    22261 cattgcctgt ggtggcgaaa ggcgatgagt tgctgtgtga aaaaggtgaa gtggtagagc
    22321 ggcaaaccca gccgccgcgc cattttaccg atgcaacact gctttcggcg atgaccggga
    22381 tcgcgcgctt tgtgcaggac aaagatctga aaaaggtcct tcgtgcgacc gatggtctgg
    22441 ggacagaagc aacccgtgcc gggattattg aactgctgtt caagcgtggt tttctgacca
    22501 aaaaagggcg ctatatccac tccaccgacg ccggaaaagc gttattccat tcgctgccag
    22561 aaatggcgac gcgaccggac atgaccgcgc actgggaatc ggtgctgacg caaatcagcg
    22621 aaaagcagtg tcgctatcag gactttatgc agccgctggt ggggacgcta tatcagctta
    22681 ttgatcaagc caaacgtacg ccggtgcggc agtttcgcgg cattgtggct ccgggcagtg
    22741 gtggcagtgc tgataagaaa aaggctgcac cgcgtaaacg tagtgcgaaa aaaagtccgc
    22801 cagcagatga agccggaagc ggggcgatag cgtaagcgag cgaatctttc gtgctattcg
    22861 agtcatattc tgaaatatcc agcggatcaa gaaaattcgt tggatatttt tttgcatgga
    22921 taaaattatc gcctctaaag tatgtaataa cagggaatgt gatgaaaaaa gttttattgc
    22981 aaaaccatcc tgggagcgag aagtattctt ttaatggctg gggaatattt aatagtaatt
    23041 ttgaacgggt gattaaagaa aataaggcca tgctgctttg taagtggggg ttttatttaa
    23101 catgtgttgt cgctgtaatg tttgtattcg cagcgataac atccaacggt ttgaatgaaa
    23161 gaggcctgat taccgcggga tgctcttttc tttatctatt aattatgatg gggcttattg
    23221 ttcgggccgg ttttaaagca aaaaaagaac aactgcatta ttatcaggct aaaggtattg
    23281 agccgctcag tatcgagaag ttacaggcgc tacaattgat cgcaccttat cgattctatc
    23341 ataagcaatg gtctgaaacg ctagagttct ggccgcgaaa gcctgaacct ggcaaagata
    23401 ccttccaata tcatgtgctt ccttttgact cgatcgatat catcagtaaa agacgcgagt
    23461 ctttagagaa tcaatggggt atcgaagata gcgaaagtta ttgtgcctta atggagcatt
    23521 ttctttctgg cgaccatgga gccaatacct ttaaagcaaa catggaggaa gccccagagc
    23581 aggttatcgc cttgttgaat aaatttgctg tttttccctc agactatatc tctgattgcg
    23641 ctaataatcg ctccggtaaa tcctcggcga agctaatatg ggcggcggaa ttatcatgga
    23701 tgatctcgat atcaagcaca gcttttcaaa acgggacaat tgaagaagaa ctggcctggc
    23761 attatataat gcttgcttct cgaaaggcgc acgagttgtt cgaaagcgaa gaagattatc
    23821 aaaaaaatag tctaatggga tttctttact ggcatatctg ctgctatcgc agaaagttaa
    23881 cggatgctga acttgaagcg tgttatcgtt acgacaagca gttttgggag cactacagta
    23941 agaaatgccg ttggcccata agaaatgttc cctggggagc atcatccgtt aaatactcat
    24001 aaacgcctga aattttgccg ggggcgctgc gcttgaccgg cctacattag agcgtaactg
    24061 caatgaattg tgcccatctg taggcctgat aagcgtagcg ccatcaggca tttacaactt
    24121 aaatcacacc ctgcgccagc atcgcatcgg caaccttcac aaaaccggca atgttcgcgc
    24181 cctgcacgta gttggtttgc tcaccttcac caccatgctc aacacaggca tggtggatat
    24241 ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc tttccagccc aggcgcgcag
    24301 cgttttgtgc catttccagg cccgacgttg ctacaccgcc agcattagcc gctttacccg
    24361 gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc ttcgatggtg gtcggcatat
    24421 ttgccccttc agcgacggct ttaacgccat tggcgataag ctgatgcgcg gcgtcaacat
    24481 ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac cggtacagac cacggctgtt
    24541 ggccttcgag atagaccaga ccaaattctt tggcgtaatc tgccactcga ccatcgcggc
    24601 tggctttgat ttcgataaga cgtgccagtt tctctttcgt gaatccgctt tcatcaacta
    24661 cagtgccgct ggagtctgac gcagtgatca cacgagcacc aaattccatc gctttttcga
    24721 tagcgtactg ggcgacgttg ccggagccag aaacggaaac gctcatccct tcaaaaccca
    24781 taccgtggcg ttttaacatt gcttctgtga aataaaccag accgtagccg gtagcttccg
    24841 ggcgaataag actaccgcca aatgaaaggc ccttaccggt gaagacgcag gcggtattgt
    24901 tggagagctt tttcatcatc cccgccataa agccgacttc acgaccacca acgccgatat
    24961 cacctgccgg aacgtcggta tccgcgccca ggtggcgata cagttcagtc atcagcgtct
    25021 ggcaaaaacg catcacttta ccttcgcttt ttcctttcgg atcgaaatcg ctgccgcctt
    25081 taccaccgcc catcggcagg gtagtcaggg catttttgaa ggtttgttca aagccgagga
    25141 atttgagaat ggaaaggtta actgacggat ggaagcgcat accgcctttg tacgggccga
    25201 tggcagagct gaactgcaca cgccatgcac ggttgacctg tatctggttg cgatcatcaa
    25261 cccataccac gcgaaactgg atcacgcgct ccggttcaac cagacgctcc agtaatgaca
    25321 tctggcgata ttttggattt tgttcaagaa aaggccagag tgtggtcatt acttcacgaa
    25381 cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg gacatggttg aggaatgact
    25441 ccagagaata tgtctgatcc atagatataa aaaccttata tattaatacg attgtgctta
    25501 tgttgtttat gtcatgtgct tttgcagttt tcgactatac catcaagaat gtgacggtaa
    25561 gcaagaaaaa aatgacccag gaaagcaaat taataacgag agtaatctca taaccaaatg
    25621 cgataactca gacgctaaag ttttgccacc cggctaccgt tatcagtagc agagacaata
    25681 acgttaagga gttccctgtg agtcgagcat tgttcgccgt agtattagca tttccattaa
    25741 ttgcactggc taacccgcat tatcgtccgg acgtggaggt aaacgtaccg ccagaagtgt
    25801 tcagttcagg cgggcagtca gcgcagccct gtactcagtg ctgcgtttat caggatcaaa
    25861 attattctga aggggcggta attaaagccg aaggaatact gctgcaatgc cagcgtgatg
    25921 acaaaaccct tagcactaat ccgctgctct ggtggcgcgt aaagccataa acgcctctaa
    25981 taatggaatg tcagcagggg ccagcggata ttgcagcgcc tcttcaggtg agcaccagac
    26041 cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag tcgggtacgt gccaggcatg
    26101 aagatggata atccggcccg aaacttctcg ctgatggctg gcaacatact cacccacagt
    26161 tgcttcgatg tccaattctt cgtttaactc acgcaccagc acctgctgct ggctttcatc
    26221 cagctcgact ttacctccgg caaactccca taatcccgcc tgatcgctct gggcggggcg
    26281 ttgcgcgagt aaaattttgc catcacgttc aatgatggcg gcaacaactt caatcatttt
    26341 cataaattac acacgggcag ataagcgaaa aatgccataa tatccgcact gttgctcaat
    26401 cagaaaggct ttatatttaa ggagtgcctg tgctagaccg ccatcttcat ccccggatta
    26461 aaccgttgtt gcatcagtgc gtgcgggttc ttgataaacc gggcattacg ccagatggtt
    26521 taacattagt gggatttgcc atcggcgtgc tggctctgcc gtttctggcg ctgggctggt
    26581 atctggcggc gctagtcgtc attttgttga acaggctgct tgatggtctg gacggtgcgc
    26641 tggcgcggcg cagagggctt accgacgcgg gcggctttct tgatatttct ctcgattttc
    26701 tcttttacgc gctggtgccg tttggcttta ttcttgctgc accggagcaa aatgcgctgg
    26761 caggcggctg gttgttgttt gcgtttatcg gcacgggcag cagttttctc gcctttgctg
    26821 cactggcagc aaaacatcag attgataacc ccggttatgc acataagtcg ttttactatc
    26881 tggggggatt aaccgaaggc acagaaacga tcttactgtt tgtgctggga tgtttattcc
    26941 ctgcatggtt tgcgtggttt gcgtggatct tcggtgcgtt gtgctggatg acgaccttta
    27001 cgcgggtgtg gagtgggtat ctgacgctga agtcgctcca gcgtcagtaa ttgcaatgtt
    27061 atttgctact gcccgggccg cgttcaccgg ttgcaaccgg attttttgga tcgctgctcc
    27121 attcgtacca gccgccgtca tacacggaaa cattcttcca acccatggcg cgtgcgtaca
    27181 taaaggtttc ggacgcgcgc cagccggtgc cgcagtagaa tgaaacttgc tgatctggtt
    27241 tgatattcca tgctttccac atagcggtaa tatcatcggc gctacgcatg gtgccatccg
    27301 ggttatagaa atcttccata tgcgtcgagt cgctaccagc gtgtccccaa cgtgctccgg
    27361 ctatttcacc ttttggttta atatagctgt aaccgctggt cgtaccgata aattctggcc
    27421 acgaacgaat gctcaccagc gatgcatcct ggcgatgcag cagtccacgg gcctgttcca
    27481 tatcgagcat caactgcggt tgtgccggga tcttcacgcc gaaatccggt tccgctttca
    27541 ctttcggtgg cgttcctcgc tcaacaggca gtcccgcgtc ggaccaggtt tgccagccgc
    27601 catccagcag acgcacatct ttcacgcctg cgtagagcat aatttgcgcc acacgcgctc
    27661 cagcgtatac gtcacgcccg tacagaatga ccgtggtgtc atggcgaatg ccgtgttttg
    27721 ccagcatcgc ttttagttgt tcatcagaaa ctttgttcca cagcggttcg ctttccactt
    27781 cgttggtatc gatgtagtca gcgccaggaa tatggctgat aaggtaaagc ttaggagcgc
    27841 cctaggccgc ttcaatgact ttccagtcac cgacaggttt cgccgtaacc tctttacctt
    27901 gttgcaggtc atgcagccat tgcggataaa ccagctgctc aaagtgcggc agtttttgta
    27961 gacgggaagg ttcgcttagc gcgtcactca gaatggagat atgcgtaaaa cctgcttttt
    28021 gcagtcgcgt tttgacggcg tcgacatctt tgccattacc gtacagcgcc accggggcat
    28081 cggctttcag gttatgttgc ttgatccacg cgttgagctg ttcggtgctc attttgtcga
    28141 gccagctagc agagaggttt aaggcggcag gttcatgacc agaagggcca tttaaggttt
    28201 gtggccagcc gttataaaac gcgctggggc gagtatctat cgctttgcca ttttgttgct
    28261 gaagttggtc aagtgtaaga ggcttcgcca gttcagcggc ccacgaagaa gcgcaagcca
    28321 gccctaaagc cattgccagc gcggtcattt gagaaacacg tttcatcgaa aatcccgaca
    28381 ttgtagtttt gagtgcgcca ttctgaactg acgcattgag aaaaactttg cgttatcgca
    28441 gtttgttgta attttctgac cactgcgcca tatccagaac agaactatca gcaggaacat
    28501 cctggagatc gtgcgttacc tgaacgacgg ggatcgccag ggcgcgaact tcgctgaaca
    28561 cccactggcg aaaattatcg cgcagagcca catcaagacg gctgaatggc tcatccagga
    28621 gtaacgcttt tggctgggcg agaagggcgc gtagtagagc aacgcgcgct cgctgaccgc
    28681 cagacaaagt ggcaggatcc tgatggaaag ctccgtcaag gcctgaacgc tcaagtgcat
    28741 cattcacggc attacgtcgg gcattcccct taagtgtcgc cggtagcgcc agcagtaaat
    28801 tttgcccgac gctgaactgg tcaaataaca gtgcatcctg aaaaagaatg ccaatctgac
    28861 gctgtgcggt gggtagcatg tcaatccgtt gctcattgag ccatagctca cctgtacaag
    28921 aaaactgtcc ggccagtgca ccaatcatcc atgaaaacag agtggatttt ccacagccag
    28981 acggccccat taacgtgacg atgtcacctt tatcaaccgt aaagttaacg tttgtcagca
    29041 agcggctttc tggtaaacgt agcgaaacat ttttcacgca gagcattagc ggattccttg
    29101 tctgacataa cctacccatt ttgcgactaa cgcggtcagg gcaaaaataa taagcggtaa
    29161 caacagttgc cataacgcct gggcggcgag aataccgttg ctgccgccgc tgcttaatgc
    29221 caccgcctca gtggtgagcg tcggaaaacg ccccgcgccc agccacagcg tcggcatata
    29281 ctgcgcaata ctgactgaaa atcccaccgc gaaggcagtc agcacagggc gcaacatgag
    29341 tgggcatttc acgtaaaaga agattttggc ccgcgaccag cccagtgttt gcgcaatcaa
    29401 aattaaccgt gaatcaatgc gctgccaggc gggttgcagg ataaacagca tccacggcat
    29461 tacccacagc agatgccccc agaccaccgc cgtccagctt ccatccaggt tcagccatag
    29521 cgccagcgtg tactggcccg ccaccagtgg cagagcgggc aatagaatgg gcaaccatag
    29581 ccacaactgg cgacgctgtg ccccccattc cagccacagc agtaacagga gcaaagcgat
    29641 gaatgttgct accagcccca ttgtcagact gttgatgagt gcttcactat tgatcgtcga
    29701 ctgatccgcg aggatcgcca gcagaaccac acacagcaca ccggttaagg gtaaaaaact
    29761 cgccagcgta gtgcctggta ataaaggtgt ggcaggcttg cgaatgccat ctacgcgggg
    29821 aatagtgcgc cgccagctgc gccaaaacgt aggcggcgag taacagcatc agcagcagac
    29881 tggcgagtgc gcctttcgtt tgttgatccg cgtcgccctg agttaaccac tgccagctaa
    29941 ttactgccag cgtcggtgga ttacctggcc caagaataat cgccacatcc acgaccgaaa
    30001 gcgaccaggc aacaatcgcc agcatcgcca ttgccagcga aggcgcgacg gagggcaaca
    30061 gcagccagtt caggcattgc cagcggctgt agcccagcga atccagcaca atgacctgct
    30121 gcaacagccg tttttcgctc aatactgcca ctaagatcca cagcagaaat gcgctttctt
    30181 tcactgcaag ggtgaggccc agaccgatcc aaatcggtcc attggcggag tgaaatacgg
    30241 gaaatagtca tatagcagcc ctccgtcagc aaagagcagc agggcgctgg tggcaaaagc
    30301 cacatgggga atggcgagca gccacggcag acgggcgcac atacgctgcc atttcggccc
    30361 aggccatagc gccacaatca ccagcagggc aatcaacaat gccccgaccg ccgcgatggt
    30421 tgtcgacacc agcgttgcca gtaatgcctg cggtaactgt ggatcggcaa ataacgcctg
    30481 ccagcgtgtc aacgacaacg caggcgatat cagcgtgaga gctgccggga tcagcggtgc
    30541 ataaatcacc gccaccatcg cccacagcaa aaagattaat gcgtaccgta acggcgtaac
    30601 cattcttgtt ccagtgcatt cacccatcct gcgtgcggtt cagccagtac cggcggcaga
    30661 tcctgcggca ttcttgattg caatatttcg cgctgcccgt caggcagttt ttgcggatcg
    30721 agaacagaag gatcgcccca gacaacggga tctgctttac gcagttgcgc atcgggtgag
    30781 agcaggaaat tggcaactac cttcgccgca gcactggcat tcgcgttggc tggagtggtg
    30841 acgaaatgca cgttgccaat catcccctcg cgaaagccaa aactgtaact gcttgccggc
    30901 aaatcgccgc tggcgatttt ttgctgcgca tgtgcggggt taaaggtcag cgacaggcgc
    30961 aatgtgccgg ctttcagcag agcatccatc cgcgcgggcg aaggcgggaa atcctttcct
    31021 tcgcgccaca aatacggatg cagtgcatca agatattgct acaagggcgc agtgacacgg
    31081 gcgaaagtcg catcgtccgg cgcttctttt aatgcggcgg gatctggcgt cagcataatc
    31141 agcaactgtt caagaaacgc cgtgccggta aagtccggtg ggcgcggata agtaaccgtg
    31201 ccgggattgg ctttagcaaa ctccagtaag gcttgcggcg tttgtggtgg ctgtggcgta
    31261 acatcgcggc gggcgataaa cgtcagttgt gcgccgcccc agggcgattc cgcaccttgt
    31321 gtcggcactg aaaaatctcc ccgcaccggc agctgtgtgt cgacatagcg ccagttgggc
    31381 agagtctccg cccagcccgt ttgtaataaa ttggcctctt t
//
LOCUS       DAJVRP010000044        31186 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVRP010000044 DAJVRP010000000
VERSION     DAJVRP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.044"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            389..2047
                     /locus_tag="OQN12_002030"
     CDS             389..2047
                     /locus_tag="OQN12_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1654353.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="OQN12_002031"
     CDS             complement(2044..3000)
                     /locus_tag="OQN12_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCS1654354.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="OQN12_002032"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="OQN12_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1654355.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="OQN12_002033"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="OQN12_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1654356.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OQN12_002034"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="OQN12_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1654357.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="OQN12_002035"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="OQN12_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1654358.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="OQN12_002036"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="OQN12_002036"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1654359.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="OQN12_002037"
     tRNA            complement(9859..9946)
                     /locus_tag="OQN12_002037"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OQN12_002038"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="OQN12_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1654360.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OQN12_002039"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="OQN12_002039"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1654361.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OQN12_002040"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="OQN12_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1654362.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OQN12_002041"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="OQN12_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1654363.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OQN12_002042"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="OQN12_002042"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1654364.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="OQN12_002043"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="OQN12_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1654365.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="OQN12_002044"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="OQN12_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1654366.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="OQN12_002045"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="OQN12_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1654367.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="OQN12_002046"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="OQN12_002046"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1654368.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="OQN12_002047"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="OQN12_002047"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1654369.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27413
                     /gene="dmsA"
                     /locus_tag="OQN12_002048"
                     /pseudo
     CDS             24969..27413
                     /gene="dmsA"
                     /locus_tag="OQN12_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27424..28041
                     /gene="dmsB"
                     /locus_tag="OQN12_002049"
     CDS             27424..28041
                     /gene="dmsB"
                     /locus_tag="OQN12_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1654370.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28043..28834
                     /locus_tag="OQN12_002050"
     CDS             28043..28834
                     /locus_tag="OQN12_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1654371.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28870..29496)
                     /locus_tag="OQN12_002051"
     CDS             complement(28870..29496)
                     /locus_tag="OQN12_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1654372.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29811..30959
                     /locus_tag="OQN12_002052"
     CDS             29811..30959
                     /locus_tag="OQN12_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1654373.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7686 a         7643 c         8378 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 gcgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ttgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct agtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacggtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatgg cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgtg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagccgcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccgctgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgatcgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgatcg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctaccgat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc
    25261 tacactaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcaccgtgtc tacaatccgg
    25321 accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca
    25381 ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt
    25441 acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc accatgaccc
    25501 gctcctggcc gccgggaaat accctggtcg cgcgcctgat gaactgctgc ggcggttatc
    25561 ttaaccatta cggtgactac tcttccgcgc aaattgcgga aggtttgaac catacctacg
    25621 gcggctgggc agatggtaac agcccgtcgg atatcgaaaa cagtaaattg gtagtgctgt
    25681 ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac tatcttgaac
    25741 aggcacgcca gaaatctaat gctcgcatga tcatcatcga tccgcgctat accgacaccg
    25801 gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc gcactggtta
    25861 acggtctggc gtacgtcatg atcactaaaa acctggtgga tcaggcattc ctcgataaat
    25921 attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc gccgaaaaat ggtcattata
    25981 aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa tgggcctcgc
    26041 aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca
    26101 aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa
    26161 cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat ggagggaaca
    26221 gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc ttggaaaacc
    26281 cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa
    26341 tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg atcaaaatga
    26401 tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg
    26461 aaatcctcca ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct
    26521 catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact
    26581 ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta
    26641 aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg
    26701 gcgttgagca acagtttact gaaggccgta cctaggaaga gtggatgcgg catctgtatg
    26761 cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc aagcagggga
    26821 tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc cgtgaagatc
    26881 cgcaggcaaa cccactgact acgccatcgg gcaaaattga gatttattcg caggcgctgg
    26941 ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca ctgccgatct
    27001 acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg ctgcagctta
    27061 caggtttcca ctataaatct cgcgttcact caacttacag caacgttgat gtgctgaaag
    27121 cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc ggtatccaca
    27181 acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag gcgaaagtga
    27241 cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg cgcctggtat gacccggatg
    27301 caaaacgtgt cgataagggc ggttgtatca acgtactgac cactcaacgt ccgtctcctc
    27361 tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa
    27421 ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc
    27481 tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag aagtcagttt ccgccgcatt
    27541 tatgaatatg ctggcggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt
    27601 gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc
    27661 ccgagcggtg cgatgcataa acgcgaagat ggttttgttg tggtcgatga agatgtatgc
    27721 attggctgcc gttactgtca tatggcttgc ccgtatggcg caccgcaata taacgaaacg
    27781 aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg
    27841 atctgtgttg aatcttgccc gctgcgggcg ctggatttcg gccctatcga cgagctgcgt
    27901 aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg
    27961 aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg
    28021 gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt
    28081 cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa
    28141 aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt
    28201 actgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga tgcgcgcctt
    28261 taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat
    28321 cttttttgct gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc
    28381 attgcgtacg ctatggctgg tggtgacgat ggttcttggc gtcgtctttg tctggatgat
    28441 ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat
    28501 gggctgggcg atgcgtctgc tgccagccat ttctgtactg gcactggtgg tgagtggcgt
    28561 gatgtcagtg atgcagggca cagagctggc aactattcat agttctgtgc agcaggccgc
    28621 agcgctggtg ccggactatg gtgcgctgat gtcctggcgg atcgtgcttt tggccgttgc
    28681 cctgtgcttg tggattgcac cacagctaaa aggttatcag cctgcggttc cgctactatc
    28741 cgtctcattc attctgcttc tggcagggga attaatcggt cgcggcgtat tctatggttt
    28801 gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg tttcggcccc
    28861 ggttctcttt tatttctgct tcgttaacgt gtcataactg gtcatcaaat tacgataatc
    28921 aggaatatgg ttagagaaca acgtcgccag tccttcaata tcattacgcc agtcacggtg
    28981 cagctcgcaa gccacgccga accatgtcat caattgcgca ccagcttgcg acatgcgatc
    29041 ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat gcatcagtca cgacaaagac
    29101 atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt cggttaccac
    29161 accggcaata attaactgtt ttttacctgt cgctttgact gcttttacaa aatcttcgtt
    29221 atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt gcatcgggga attgtgcttt
    29281 cagttctaga actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa gaatagttgg
    29341 taagttgaaa tacttagcca ggtcacccag cgccagcacg ttatttttaa acttatcggg
    29401 ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga tcaacaagca aaacggcagc
    29461 atcattttta tcaagacgaa catacggttt ggtcatcctc ttctcctttc gagagtcagt
    29521 ggtacgtctg aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg
    29581 agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc
    29641 aataaaaaca atcagatgag attaatcatc tcttcagcat tagcgttatg aatgagtaat
    29701 ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt
    29761 ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt
    29821 atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg
    29881 tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca tggcaggtag
    29941 gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg
    30001 tcattaaacg cattggcttt aaccgcagct attatctgtc ctccttcatt tttgctgctg
    30061 gctgtgccgg ccttggcctg atgattggtt tctggagctg gttggcgtgg cgttttgtcg
    30121 cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg tgcagtggga
    30181 cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac gtgggaacgt
    30241 ttttaggcca gttactggtc agcaaagtct caaccgagct gatgagcgta ttgccgtggg
    30301 ttacaggttt gacattggca gggatcttac cgctgttgtt tacgcgtgtc ctgaatcagc
    30361 aggctgaaaa ccatgattcg acgtcaatta cggcaatgct aaaactccgt caggcgcggc
    30421 ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg atctctatat ggcctgatgc
    30481 ctctgtacct caatcacaaa ggggtgagca atgccagtat tggtttctgg atggcggtac
    30541 tggtcagtgc gggtatcctc ggacaatggc cgattggacg tctggcggat aagtttggtc
    30601 gactgctggt tttgcgtgtt caggtctttg tcgtcattct cggcagtatt gcgatgctta
    30661 gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc
    30721 cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc
    30781 aagccttact gttgagctat accgtgggaa gtctgcttgg cccgtcattt agcgctatgc
    30841 taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta tcgtttatct
    30901 atttgctgat gctgcttcgc aacgccggtc atacgccgaa acccgttgct cacgtgtaaa
    30961 tgaattcaag caaagtgtga acttactgtt tcacactctg cttttttgtt tcttctatct
    31021 gacttgcttt tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag gttataattt
    31081 tgcatttcgc tatttccgca cttcttattt gccgcgcata atccctcgtt ttacagatgc
    31141 ccctttaatt ttggcgaagg atttgtctat ggctgggaat gttcag
//
LOCUS       DAJVRP010000045        30114 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVRP010000045 DAJVRP010000000
VERSION     DAJVRP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.045"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OQN12_002053"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OQN12_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1654374.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OQN12_002054"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OQN12_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1654375.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OQN12_002055"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OQN12_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1654376.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OQN12_002056"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OQN12_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1654377.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OQN12_002057"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OQN12_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1654378.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OQN12_002058"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OQN12_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1654379.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OQN12_002059"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OQN12_002059"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1654380.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OQN12_002060"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OQN12_002060"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1654381.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAIHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OQN12_002061"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OQN12_002061"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1654382.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OQN12_002062"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OQN12_002062"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1654383.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OQN12_002063"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OQN12_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1654384.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OQN12_002064"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OQN12_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCS1654385.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OQN12_002065"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OQN12_002065"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1654386.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OQN12_002066"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OQN12_002066"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1654387.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OQN12_002067"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OQN12_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1654388.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OQN12_002068"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OQN12_002068"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1654389.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OQN12_002069"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OQN12_002069"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1654390.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OQN12_002070"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OQN12_002070"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1654391.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OQN12_002071"
     CDS             21622..22212
                     /locus_tag="OQN12_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1654392.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OQN12_002072"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OQN12_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1654393.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OQN12_002073"
     CDS             22684..23379
                     /locus_tag="OQN12_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1654394.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OQN12_002074"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OQN12_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1654395.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OQN12_002075"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OQN12_002075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1654396.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OQN12_002076"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OQN12_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCS1654397.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OQN12_002077"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OQN12_002077"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1654398.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OQN12_002078"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OQN12_002078"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1654399.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7063 a         7847 c         8277 g         6927 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgattcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacttg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct attcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatgtcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa gagaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
    30061 tacgccacct cgcgacagtg cgctaaagcg cgtcgcaact gctctttaac aatt
//
LOCUS       DAJVRP010000046        29798 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVRP010000046 DAJVRP010000000
VERSION     DAJVRP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.046"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            56..850
                     /locus_tag="OQN12_002079"
     CDS             56..850
                     /locus_tag="OQN12_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1654400.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="OQN12_002080"
     CDS             872..2281
                     /locus_tag="OQN12_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1654401.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="OQN12_002081"
     CDS             2259..3038
                     /locus_tag="OQN12_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1654402.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3035..3895
                     /locus_tag="OQN12_002082"
     CDS             3035..3895
                     /locus_tag="OQN12_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1654403.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="OQN12_002083"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="OQN12_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="OQN12_002084"
     CDS             complement(4635..4895)
                     /locus_tag="OQN12_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1654404.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="OQN12_002085"
     CDS             complement(4886..6157)
                     /locus_tag="OQN12_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1654405.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6597)
                     /gene="queD"
                     /locus_tag="OQN12_002086"
     CDS             complement(6235..6597)
                     /gene="queD"
                     /locus_tag="OQN12_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1654406.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="OQN12_002087"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="OQN12_002087"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1654407.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="OQN12_002088"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="OQN12_002088"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1654408.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="OQN12_002089"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="OQN12_002089"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1654409.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="OQN12_002090"
     CDS             11499..11651
                     /locus_tag="OQN12_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS1654410.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12936
                     /locus_tag="OQN12_002091"
                     /pseudo
     CDS             11845..12936
                     /locus_tag="OQN12_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="OQN12_002092"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="OQN12_002092"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1654411.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="OQN12_002093"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="OQN12_002093"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1654412.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="OQN12_002094"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="OQN12_002094"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1654413.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="OQN12_002095"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="OQN12_002095"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1654414.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="OQN12_002096"
     CDS             17363..17686
                     /locus_tag="OQN12_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1654415.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="OQN12_002097"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="OQN12_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1654416.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="OQN12_002098"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="OQN12_002098"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1654417.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="OQN12_002099"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="OQN12_002099"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1654418.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..20445
                     /gene="truD"
                     /locus_tag="OQN12_002100"
     CDS             19396..20445
                     /gene="truD"
                     /locus_tag="OQN12_002100"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1654419.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20426..21187
                     /gene="surE"
                     /locus_tag="OQN12_002101"
     CDS             20426..21187
                     /gene="surE"
                     /locus_tag="OQN12_002101"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1654420.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21181..21807
                     /gene="pcm"
                     /locus_tag="OQN12_002102"
     CDS             21181..21807
                     /gene="pcm"
                     /locus_tag="OQN12_002102"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1654421.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21947..23086
                     /gene="nlpD"
                     /locus_tag="OQN12_002103"
     CDS             21947..23086
                     /gene="nlpD"
                     /locus_tag="OQN12_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1654422.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23149..24141
                     /gene="rpoS"
                     /locus_tag="OQN12_002104"
     CDS             23149..24141
                     /gene="rpoS"
                     /locus_tag="OQN12_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1654423.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24261..24668)
                     /locus_tag="OQN12_002105"
     CDS             complement(24261..24668)
                     /locus_tag="OQN12_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1654424.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24815..25408
                     /locus_tag="OQN12_002106"
     CDS             24815..25408
                     /locus_tag="OQN12_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCS1654425.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25408..26355
                     /locus_tag="OQN12_002107"
                     /pseudo
     CDS             25408..26355
                     /locus_tag="OQN12_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26628..27191)
                     /locus_tag="OQN12_002108"
     CDS             complement(26628..27191)
                     /locus_tag="OQN12_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654426.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27356..27607
                     /locus_tag="OQN12_002109"
     CDS             27356..27607
                     /locus_tag="OQN12_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1654427.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27609..29705
                     /locus_tag="OQN12_002110"
     CDS             27609..29705
                     /locus_tag="OQN12_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1654428.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7300 a         7382 c         8058 g         7058 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggcgtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggctgc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacca gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacactccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ttggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcaattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacacccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggatcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt caggggcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacggc acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccggataa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggct cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatggacaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctc gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac
    20341 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
    20401 gaacttatca acacaacagg tgattatgcg catattgctg agtaatgatg acggggtaca
    20461 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
    20521 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg
    20581 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt
    20641 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
    20701 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
    20761 aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga
    20821 cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
    20881 cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg
    20941 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
    21001 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac
    21061 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac
    21121 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
    21181 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
    21241 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
    21301 gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag
    21361 ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
    21421 gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
    21481 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
    21541 gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg
    21601 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
    21661 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
    21721 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
    21781 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
    21841 ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
    21901 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
    21961 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
    22021 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
    22081 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga tgtctacagc
    22141 gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca
    22201 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    22261 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    22321 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    22381 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    22441 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    22501 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    22561 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    22621 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    22681 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    22741 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    22801 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    22861 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    22921 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    22981 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    23041 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    23101 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    23161 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    23221 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    23281 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    23341 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    23401 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    23461 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    23521 ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    23581 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    23641 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    23701 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    23761 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    23821 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    23881 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    23941 gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa
    24001 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    24061 attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc
    24121 gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    24181 gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt
    24241 cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc
    24301 tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt
    24361 atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg
    24421 tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct
    24481 gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt
    24541 ttatcagcaa tggcacgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc
    24601 tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt
    24661 aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga
    24721 agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat
    24781 aacgttcgta tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg
    24841 ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt
    24901 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    24961 cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc
    25021 gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa
    25081 aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt
    25141 cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    25201 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    25261 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt
    25321 gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat taccgcaggc
    25381 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    25441 gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat
    25501 ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt
    25561 gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    25621 aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag
    25681 tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg
    25741 gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc
    25801 ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    25861 ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc
    25921 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    25981 gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc
    26041 acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    26101 ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg
    26161 ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg
    26221 ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat
    26281 aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc
    26341 gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    26401 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    26461 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    26521 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    26581 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    26641 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    26701 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    26761 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    26821 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    26881 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    26941 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    27001 tttaactgat gtgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    27061 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    27121 aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt
    27181 gcttttctca ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa
    27241 taaaccataa aaacaccaaa gaacccttta cagggaatct ttggtatctt aatctttatc
    27301 catacggata tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag
    27361 acaaagagaa gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga
    27421 taacgggtgg tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact
    27481 gaaagcggta gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg
    27541 tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc
    27601 gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag
    27661 ccaccacaaa aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta
    27721 gtcgccgtcg ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa
    27781 cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt
    27841 ctgaagtgaa agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag
    27901 catatcgcgg ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc
    27961 gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg
    28021 gtaaccccgg gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc
    28081 tgccggagcg cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta
    28141 ctatcagccg cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg
    28201 ccgaacggct gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca
    28261 gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag
    28321 cctacgagga ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg
    28381 aggtgatgcc ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga
    28441 aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg
    28501 actggacagc tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga
    28561 aatgtgcgca tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg
    28621 gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg
    28681 ccggtgcgat acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata
    28741 acggttccgg tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc
    28801 tggggattaa acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac
    28861 gcctgaaccg gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg
    28921 gtgctgaccg ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc
    28981 tgcaacagga caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt
    29041 gggcggagct tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt
    29101 ctgagctgcc gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac
    29161 gccaggcaga agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt
    29221 tccgcccaat ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt
    29281 actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc
    29341 atgacgccga gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat
    29401 ggaacggcaa taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc
    29461 gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta
    29521 ttcagattct ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg
    29581 gtaatgtggt ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac
    29641 gcgatttccg gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg
    29701 cctgaataaa aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt
    29761 gaaataaccg aggaaatact atgactgata ttgatgat
//
LOCUS       DAJVRP010000047        29638 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVRP010000047 DAJVRP010000000
VERSION     DAJVRP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.047"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..188)
                     /locus_tag="OQN12_002111"
     CDS             complement(<1..188)
                     /locus_tag="OQN12_002111"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCS1654429.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
     gene            complement(329..2362)
                     /gene="metG"
                     /locus_tag="OQN12_002112"
     CDS             complement(329..2362)
                     /gene="metG"
                     /locus_tag="OQN12_002112"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS1654430.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2494..3603
                     /gene="apbC"
                     /locus_tag="OQN12_002113"
     CDS             2494..3603
                     /gene="apbC"
                     /locus_tag="OQN12_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1654431.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3865..4146
                     /locus_tag="OQN12_002114"
     CDS             3865..4146
                     /locus_tag="OQN12_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS1654432.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4439..4963
                     /locus_tag="OQN12_002115"
     CDS             4439..4963
                     /locus_tag="OQN12_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1654433.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5044..5718
                     /locus_tag="OQN12_002116"
     CDS             5044..5718
                     /locus_tag="OQN12_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1654434.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5734..8214
                     /locus_tag="OQN12_002117"
                     /pseudo
     CDS             5734..8214
                     /locus_tag="OQN12_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8230..9263
                     /locus_tag="OQN12_002118"
                     /pseudo
     CDS             8230..9263
                     /locus_tag="OQN12_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9345..9683)
                     /gene="rcnB"
                     /locus_tag="OQN12_002119"
     CDS             complement(9345..9683)
                     /gene="rcnB"
                     /locus_tag="OQN12_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS1654435.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9902..10750)
                     /gene="rcnA"
                     /locus_tag="OQN12_002120"
                     /pseudo
     CDS             complement(9902..10750)
                     /gene="rcnA"
                     /locus_tag="OQN12_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10872..11144
                     /gene="rcnR"
                     /locus_tag="OQN12_002121"
     CDS             10872..11144
                     /gene="rcnR"
                     /locus_tag="OQN12_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCS1654436.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11236..11332
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11367..12155
                     /gene="thiM"
                     /locus_tag="OQN12_002122"
     CDS             11367..12155
                     /gene="thiM"
                     /locus_tag="OQN12_002122"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1654437.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12152..12952
                     /gene="thiD"
                     /locus_tag="OQN12_002123"
     CDS             12152..12952
                     /gene="thiD"
                     /locus_tag="OQN12_002123"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1654438.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13017..13838
                     /locus_tag="OQN12_002124"
     CDS             13017..13838
                     /locus_tag="OQN12_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1654439.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13886..14632
                     /locus_tag="OQN12_002125"
     CDS             13886..14632
                     /locus_tag="OQN12_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1654440.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14606..15571)
                     /locus_tag="OQN12_002126"
     CDS             complement(14606..15571)
                     /locus_tag="OQN12_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCS1654441.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15568..16571)
                     /locus_tag="OQN12_002127"
                     /pseudo
     CDS             complement(15568..16571)
                     /locus_tag="OQN12_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16568..17845)
                     /locus_tag="OQN12_002128"
     CDS             complement(16568..17845)
                     /locus_tag="OQN12_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1654442.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18102..19154
                     /gene="fbaB"
                     /locus_tag="OQN12_002129"
     CDS             18102..19154
                     /gene="fbaB"
                     /locus_tag="OQN12_002129"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1654443.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19462..20316
                     /gene="gatY"
                     /locus_tag="OQN12_002130"
     CDS             19462..20316
                     /gene="gatY"
                     /locus_tag="OQN12_002130"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1654444.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20345..21607
                     /gene="gatZ"
                     /locus_tag="OQN12_002131"
     CDS             20345..21607
                     /gene="gatZ"
                     /locus_tag="OQN12_002131"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCS1654445.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21617..22069
                     /gene="gatA"
                     /locus_tag="OQN12_002132"
     CDS             21617..22069
                     /gene="gatA"
                     /locus_tag="OQN12_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1654446.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22100..22384
                     /gene="gatB"
                     /locus_tag="OQN12_002133"
     CDS             22100..22384
                     /gene="gatB"
                     /locus_tag="OQN12_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1654447.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22388..23743
                     /locus_tag="OQN12_002134"
     CDS             22388..23743
                     /locus_tag="OQN12_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS1654448.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23791..24831
                     /gene="gatD"
                     /locus_tag="OQN12_002135"
     CDS             23791..24831
                     /gene="gatD"
                     /locus_tag="OQN12_002135"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1654449.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24937..25710
                     /locus_tag="OQN12_002136"
     CDS             24937..25710
                     /locus_tag="OQN12_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1654450.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25792..26691)
                     /gene="yegS"
                     /locus_tag="OQN12_002137"
     CDS             complement(25792..26691)
                     /gene="yegS"
                     /locus_tag="OQN12_002137"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCS1654451.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27097..27414
                     /locus_tag="OQN12_002138"
     CDS             27097..27414
                     /locus_tag="OQN12_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654452.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27756..29117)
                     /gene="yegQ"
                     /locus_tag="OQN12_002139"
     CDS             complement(27756..29117)
                     /gene="yegQ"
                     /locus_tag="OQN12_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1654453.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29264..29595)
                     /locus_tag="OQN12_002140"
                     /pseudo
     CDS             complement(29264..29595)
                     /locus_tag="OQN12_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         7702 a         6879 c         7477 g         7580 t
ORIGIN      
        1 cccttcttca ccttctccgc aatcagcttc agttccgctt tttccgccgc cgcagcatct
       61 gaaaaacttt ttatctgact ttgccctttg gtgccaactt ttccccaact gatatgcaac
      121 tcgtttccct gttgctccac cgtccagaat ttatgtgatt tttcgtcctg atagataaag
      181 tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac cagtatttat
      241 acgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata aagaaaaggc
      301 gctgtcgatg cagcgccttg aagggggatt atttcacctg atgacccggt ttagcaccgg
      361 catccgggct tagcaggaaa atatctttcc cgccaggacc ggcagccatc accatgcctt
      421 cagagatacc gaagcgcatt ttacgtggtg ccaggttagc caccataatg gtgtgacgac
      481 caatcagtgc ctgcggatcc gggtaagcag aacgaatacc ggagaagaca ttgcgttttt
      541 caccgccgag atccagcgtc aggcgcagca gtttgtcaga accttcaaca aactctgcgt
      601 tttcgatcag cgccacgcgc aggtcaactt tggcgaagtc gtcaaaggtg atggtttcct
      661 gaatcggatc atctgccagt gggccagtaa ctggcgcggt agtggctttc acttcttctt
      721 tagaggcttc caccagagct tcaacctgtt tcatatcgat gcggttatac agcgccttga
      781 acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc agttccgtat
      841 tgaggaaggc ttctgcacgc tcggtcagtt tcggcagtac cggcttcagg taagtcatca
      901 gcacgcggaa caggttgatg cccattgagc aaatcgcctg caggtcagca tcgcgacctt
      961 cctgttttgc gactacccac ggagcctgct cgtcgacata gcggttagcc aggtcagcca
     1021 gtgccatgat ttcgcgcact gctttaccaa attcacggct ttcccacgct tcaccaatca
     1081 cttcagcggc atcggtaaag gttttgtaca gctcagggtc agccagctcg cttgccagca
     1141 caccgtcaaa acgcttgttg ataaagcccg cattacggga ggccaggtta accactttgt
     1201 tcacgatatc ggcattcaca cgctgaacga aatcttccag gttgagatcg atatcatcaa
     1261 tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca aaatgattca
     1321 gccaggtgct ggctttaata aaggtgccgc gagacttgga catcttcgcg ccgttcaccg
     1381 tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct tccagcatgg
     1441 caggccagaa caggctgtgg aagtaaacga tatctttacc gatgaagtgg tacagctcgg
     1501 cggtggagtc tttcttccag tattcatcga agcttacgct gtcgccgcgc ttgtcgcaca
     1561 gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag aaatatttgc
     1621 ccggtgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata tcccactgtt
     1681 gcaggccgga ttcaaaccac tcctgcattt tgtttgccac ctgctcctgc aacgcaccgc
     1741 tgcgggtcca tgcctgcagc atttcgctga aagagggcag atcaaagaag aagtgttcag
     1801 aatcacgcat cactggtgta gcaccagaaa ccaccgattt cggctcgatc aattcagtcg
     1861 ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg gatttacatt
     1921 tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc ggatcgtaca
     1981 gctgagagat ggtgcggttt ttaataaaac cgttttcttt caggcgagag tagataagtt
     2041 ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa
     2101 agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc tccggggtga
     2161 taccgagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga
     2221 agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc tggatgtgct
     2281 ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa
     2341 ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa ggggttagat
     2401 agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt aaactatcag
     2461 ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca
     2521 ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg
     2581 aagcataacc tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg
     2641 catgtggaac tggttatgcc gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa
     2701 tgtagcgccg aactgctgcg catcaccggg gcgaaagcta tcgactggaa actgtcgcat
     2761 aacatagcca ctctgaaacg cgtgaagaat cagccgggaa ttaacggtgt gaagaacatt
     2821 atcgcggtga gttccggtaa aggcggcgtg ggtaaatcgt ccacggcggt aaacctggcg
     2881 ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat ctacggccca
     2941 tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga cggtacccac
     3001 atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct ggtcactgac
     3061 gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca gatgttgcag
     3121 gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg caccggtgac
     3181 attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt caccaccccg
     3241 caggacatcg cgttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt
     3301 ccggtgctcg gtattgtcga gaacatgagt gtgcatattt gcagtaactg tggtcaccat
     3361 gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca tacacagctg
     3421 ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg cacgccaacg
     3481 gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc tgaccgcgtt
     3541 gcagctcagc tctactggca gggtgaagtc attcctggcg agatttcctt ccgcgcggtc
     3601 taacgcctat taaccattct ggttattctt catacaccgc agagatgtta tattgatgcg
     3661 gtgtatttgt ttttgccgca ttaatgaaca cttatttaat actgcgaata ttctgcggac
     3721 cttcgtccga aactctacaa agtcgagtga tcgtacaggc aatgcttcat tatcatcctt
     3781 cgtgctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat ccaggcataa
     3841 cctttactac agggataacg ttcaatgaat aagtattggc tgtcaggaat tatttttctt
     3901 gcctatggat tggcttctcc cgcagtctct tcagagactg ccacattaac gattaatggc
     3961 aggatcagtc ccccaacatg cagtatggct atggttaata gtcaaccaca acagcattgt
     4021 ggtcagttaa cgtataatgt tgacacccgt catcaggttt cttcgcccgt caaaggtgtt
     4081 acgacagaag tggttgttgc aggtagcgat agcaaacgtc gaatcgtgtt aaatcgctac
     4141 gattaatttg gcaacctgat tttaattgca tttagaaaga cgaaatcatt tatataaatg
     4201 atttcgtctt ttttattgat atcaacaata ccatttacat aaatctacat taattaatag
     4261 atgtgataaa tatttaatat tcttaagtat ttgttgtatt tatattgcat tggttaattt
     4321 gttttaaatc aataaaacaa tatgtttaca ttgcggcagc ttttttgatg tgagaaattt
     4381 gtttccaaga gatacagact cttaacaacc aaaaataaat tttattaagg aacatattat
     4441 gaaacgttca attattgctg ccgctgtctt ttcttctttt tttatgagcg ctggagtatt
     4501 tgctgcagac gttgataccg gaacattaac cattaaagga aatattgcag aatctccgtg
     4561 taaattcgaa gcgggtggtg attcagtaag tattaatatg ccgactgtac caaccactgt
     4621 ctttgaaggt aaagctaaat attctaccta tgatgatgca gtcggtgtaa ccagcagcat
     4681 gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa ctctcgttga tcacaaacga
     4741 caaaataacc ggtaacgata aggcgatagc cagtagcaac gataccgtag gtgataacag
     4801 cgatgtccta gatgtttctg caccttttaa cattgagagt tataaaacag cggaaggtca
     4861 atatgctatt ccgtttaaag caaaatacct gaaactgaca gataactcag tgcaatcagg
     4921 tgatgtgtta tcttctctgg ttatgcgtgt ggcgcaggat taataaataa aattgcaagt
     4981 ttctccaggt aataaacatg gctgctatcc ccatggcggc tttttaattt aagaggcatt
     5041 aagatgaaag gattattatc tttactcatt ttttctatgg tccttcctgc acatgccgga
     5101 attgttatct acgggacgcg cattatttac ccggcagaaa ataaagaagt tatggtgcag
     5161 ttgatgaacc agggaaatcg ttcttcgctg ctgcaggcgt ggattgatga tggcgatacg
     5221 tcgttaccac cagaaaaaat tcaggttcct ttcatgttaa cgccaccagt ggcaaaaata
     5281 ggggcaaatt ccgggcagca agtaaaaatc aaaattatgc cgaataaact gcccactaat
     5341 aaagaaagca ttttttatct gaatgttctg tatattccac caaatagtcc agagcaagaa
     5401 ggcaagaatg cactgaagtt tgcgatgcaa aacagaatta agttgtttta ccggccagcg
     5461 ggtattgctc cggtaaataa agcgaccttt aaaaagttac tggtaaatcg cagtggcaat
     5521 ggattggtga taaaaaatga ctcagctaat tgggtgacga tttcggatgt caaagctaat
     5581 aatgttaaag tcaattatga aactattatg attgccccct tagaaagtca gagtgttaat
     5641 atcaaaagta ataatgcaaa taactggtat ctgaccatta tcaatgacca cggcaactat
     5701 attagtgaca aaatttaatt gcaggagctg cctatgttga gaatgacccc acttgcatca
     5761 gcaatcgtag cgttattgct cggcattgaa gcttatgcag ctgaagaaac ctttgatacc
     5821 cattttatga taggtggaat gaaagaccag caggttgcaa atattcgtct tgatgataat
     5881 caacccttac cggggcagta tgacatcgat atttatgtca ataagcaatg gcgcgggaaa
     5941 tatgagatta ttgttaaaga caacccgcaa gaaacatgtt tatcaagaga agttatcaag
     6001 cggttaggca ttaatagcga taacttcgcc agcggtaagc aatgtttaac atttgagcaa
     6061 cttgttcagg gtgggagcta tagctgggat atcggggttt ttcgtctcga tttcagtgtc
     6121 ccgcaggctt gggtggaaga actggaaagt ggctatgttc caccggaaaa ctgggagcgg
     6181 ggtattaatg cgttttatac ttcttattat gtgagtcagt attacagcga ctataaagcg
     6241 tcgggtaaca acaagagtac atatgtacgt tttaacagcg ggttaaattt actggagtgg
     6301 caactgcatt ctgatgccag tttcagtaaa acaaataaca atccaggggt gtggaaaagc
     6361 aataccctgt atctggaacg tggatttgcc caatttctcg gcacgcttcg cgtgggtgat
     6421 atgtacacat caagcgatat ttttgattct gttcgcttca gcggtgtgcg gttgtttcgt
     6481 gatatgcaga tgttgcctaa ctcgaaacaa aattttacgc cacgggtgca ggggattgct
     6541 cagagtaacg cgctggtaac tattgaacag aatggttttg tggtttatca gaaagaggtt
     6601 cctcctggcc cgtttgcgat tacagatttg cagttggccg gtggtggagc agatcttgat
     6661 gttagcgtga aagaggcgga cggctcggta accacctatc tggtgcctta tgcagcggtg
     6721 ccaaatatgc tgcaacccgg cgtgtcgaaa tatgattttg cggcgggccg tagccatatt
     6781 gaaggggcga gcaagcaaag tgattttgtc caggcaggtt atcagtatgg ttttaataac
     6841 ttattgacgc tgtatggcgg cacgatggtt gctaataatt actatgcgtt taccctctga
     6901 acgggttgga acacgcgcat tggcgcaatt tccgtcgatg ccacgaagtc gcatagtaaa
     6961 caagacaacg gcgatgtgtt tgacgggcaa agttatcaaa ttgcctacaa caaatttgtg
     7021 agccaaacgt cgacgcgttt tggtctggcg gcctggcgtt attcgtcgcg tgattaccgg
     7081 acatttaacg atcacgtttg ggcaaacaat aaagataatt atcgccgtga tgaaaacgat
     7141 atctatgaca ttgccgatta ttaccagaac gattttggcc gtaaaaatag cttttctgcc
     7201 aatatgagcc agtcattgcc agaaggctgg ggttctgtgt ccttaagtac gttatggcga
     7261 gattactggg ggcgtagcgg cagcagtaag gattatcagt tgagttattc caataactgg
     7321 cggcggataa gctataccct cgcggcaagc caggcgtatg gcgagaatca tcatgaagag
     7381 aaacgtttta atatttttat atcaattccc tgtgattggg gtgatgacgt tacgacgcct
     7441 cgtcggcaaa tatatatgtc taactcaacg acgtttgatg atcagggttt tgcctcaaat
     7501 aatacgggat tatcaggaac agtagggagt cgggatcagt tcaattatgg tgtcaacctg
     7561 agtcatcaac atcagggaaa tgaaacgaca gctggggcga atttgacctg gaacgcgccg
     7621 gttgcgacag tgaatggcag ttatagtcag tcgagtactt atcgacagac tggagccagt
     7681 gtttcagggg gcattgtcgc ctggtcgggt ggcgttaatc tggcgaaccg tctttccgaa
     7741 acgtttgctg tgatgaatgc gccaggaatt aaagatgctt atgtcaatgg gcaaaaatat
     7801 cgcacaacaa accgtaatgg agtggtggta tacgacggaa tgacacctta tcgggaaaat
     7861 cacctgatgc tggatgtgtc gcaaagcgat agcgaagcag aattacgtgg caaccggaaa
     7921 attgccgccc cttatcgcgg cgcggttgta ctggttaatt ttgataccga tcagcgcaag
     7981 ccctggttta taaaagcgtt aagagcggat ggtcaaccat taacgtttgg ttatgaagtc
     8041 aatgatatcc atggtcataa tattggcgtt gtcggccagg gaagccagtt atttattcgc
     8101 accaatgaaa taccgccatc ggttaatgta gcaattgata agcaacaagg actttcatgc
     8161 acaatcacct tcggtaaaga gattgatgaa agtagaaatt atatttgcca gtaatagtca
     8221 ggtggttcta tggaaattcg cataatgcta tttatattaa tgatgatggt tatgcctgtg
     8281 agctatgcgg catgttatag tgagttatct gttcagcaca atttggttgt tcaggggaat
     8341 tttgcactta ctcaaacaca aatagcgaca tatgagcata attttaatga ttcgtcatgc
     8401 gtgagtacaa atactatcac ccctatgagc ccatcggata ttattgttgg actttataac
     8461 gataccatta aattaaattt acattttgaa tggaccaata aaaacaacat cacgttgtca
     8521 aataatcaga gcagtttcac cagtggttat tcggtaacgg tgacacctgc ggccagtaat
     8581 gcaaaagtga atatttctgc ggggggcggt ggttcagtga tgattaatgg tgttgcgaca
     8641 ttatccagtg cttcatcatc gacacgcggg agtgccgcag tacaatttct actgtgttta
     8701 ttaggtggca agtcatgggg tgcatgtgta aatagctaca gaaatgcatt ggcacaaaat
     8761 gcaggtgtct attccttcaa tctgacattg tcatacaacc cgataaccac aacctgcaaa
     8821 caggacgatt tattaattac tttagacagt attcccgttt cacaattacc agccacaggt
     8881 aacaaagcaa caataaatag taaaaaaagg gatattattc tgcgttgtaa aaatttatta
     8941 ggtcaacaaa atcaaacatc acggaaaatg caggtgtatt tatcaagttc tgacttgtta
     9001 accaacagca acacaatact gaaaggtgcg gaagataatg cgtaggattt attcttgaaa
     9061 gtaatggttc gccagtcaca cttttaaata tcactaacag cagtaaagga tacacaaatt
     9121 taaaagaaat tgcggcaaag tcaaaactta cagatacaac ggtttcaatt ccgataacag
     9181 ccagttacta cgtctacgat acaaacaaag ttaaatctgg cgcactggag gcaaccgcat
     9241 taatcaacgt gaaatacgac taataacaag caaaacgagc aagtggctaa taataaaaaa
     9301 taacaaggtg tctgcatttc cccctcatga tgagggggct ttttttagcg atgataaaaa
     9361 atctcaccgt cgtaggcttt aatgatttta ccgtcggtgt cgctgatcaa cacgtacgcg
     9421 ccacccatat aggtccagtg cgtcccggca tcaggcgcgg gcaggttacg taactgccac
     9481 tgcttaatgt tgtactcatc ggtgcggtac atctctggga cggtatcacc tggtttgaaa
     9541 tgcttaaagt cagcaatgaa actacttaac tcatacttag aaattcccga ggtattggtc
     9601 gaacccgcgg tggctggtgc ggcccaggcg gcactggtaa ccagcaaaag cacacccagc
     9661 aacatcttat ttttaatggt cataaactct ccactttggg gatctgtaat atcagtcgca
     9721 tatctttcct gtcatacaat ataactgaca agcgatattt tgttagtgat cccatgattt
     9781 cgtaaaaagt gtaaatgaaa ggatgcccta aaagcatcct tagcgagcag agagtcagcg
     9841 gaaatagctg atttaagaat catcctgggc tgaagataac atgcaagcct aaaggattga
     9901 gttatcgcat tatgcccatg aagccatgta cgcccatata cacaccgact aagccaatca
     9961 acagactgga aaaatagggg gcgcgtttag cgagagtgtt aaatccgctc cagcgttttg
    10021 cgacctgctg aacgctgatt gctgcgccaa cgcctacggt gacaagcgtt aacgccaggc
    10081 caatgctgaa actgacgacc agtgttgcgc ccagtgtcag ggctttcaac tgaatgcata
    10141 tcaacagcac ggtaattgct gccgggcagg ggataaagcc accggttaag ccaaataaca
    10201 aaatttgcca gttgatgacc tctctaccat caaagcgtcg tttaatgtca ttggcatggg
    10261 ctcgtgcatg ggcatcctga tactcgccat attcgtgatg gtgatgatgt ccttggtcgt
    10321 ggtgatgttc gtggtcttga tgatgttcgt gatcgtgatg atggtgatga tgctcataat
    10381 catgcccgtg catattctcc agccagttgc gttcgccgcg ctaggtacgc cagaacatcc
    10441 agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt tctgctgatt
    10501 gagcagtaaa gcgcttactg atcaccatcc cgccaaaggc aatcaaccag accaccgcgg
    10561 tatgcgaaat ggttgctgcc agtccgagca tcaccgcctg tttaatggtg cctttgatgg
    10621 cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca tgaagcgcac
    10681 caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga agagttgtaa
    10741 attcggtcat gataataatt cttagtatta attcggcaat ctgattctac tcccccccag
    10801 tacctgatac taccccccca gtagattaat agtgctatga tttttcatat tcttgttaac
    10861 caggtgttgc catgtctcat acaatccgtg ataaacagaa actgaaagcg cgtgccagta
    10921 agattcaggg ccaggtcgtg gcgctcaaga aaatgctcga cgagccgcac gaatgcgctg
    10981 cagttttaca acagattgct gctatccgtg gagcggtaaa cggtctgatg cgggaagtga
    11041 ttaaaggtca tctgacggaa cacatcgttc accaggggga tgagctaaaa cgtgaagaag
    11101 atctggatgt cgttctgaag gtgctggatt catatatcaa ataatttatt aacgcgattg
    11161 taaaactgcc gtttttcctc gtttacaacg ggggcgctgg acattaccat cctcctctgc
    11221 gatttatcat cgcaaccaaa cgactcgggg tgcccttctg cgtgaaggct gagaaatacc
    11281 cgtatcacct gatctggata atgccagcgt agggaagtca cggaccacca ggtcattgct
    11341 tcttcacgtt atggcaggag caaactatgc aagtcgacct gctgagttca gcgcaatctg
    11401 cgcacgcgtt acaccttttt caccaacatt cccctcttgt gcactgcatg accaatgatg
    11461 tggtgcaaac ctttaccgcc aataccttgc tggcgctcgg tgcatcgcca gcgatggtta
    11521 tcgaaaccga agaggccagt cagtttgcgg ctatcgccag tgccttgttg attaacgttg
    11581 gcacactgac gcagccacgc gcacaggcga tgcgtgctgc cgttgagcaa gcaaaaagct
    11641 ctcaaacacg ctggacgctt gatccagtag cggttggtgc gctcgattat cgccgccatt
    11701 tttgtcatga acttttatct tttaaaccgg cagcgatacg tggtaatgct tcggaaatca
    11761 tggcattagc tggcattgct aatggcggac ggggagtgga taccactgac gccgcagcta
    11821 acgcgatacc cgctgcacaa acactggcac gggaaactgg cgcaatcgtc gtggtcactg
    11881 gcgaggtgga ttatgttacc gatggacatc gtatcattgg tattcacggt ggcgatccgt
    11941 taatgaccaa agtggtagga actggctgtg cattatcggc ggttgtcgct gcctgctgtg
    12001 cgttaccagg cgatacgctg gaaaatgtcg catctgcctg tcactggatg aaacaagccg
    12061 gagaacgcgc agtcgccaga agcgaggggc caggcagttt tgttccacat ttccttgatg
    12121 cgctctggca attgacgcag gaggtgcagg catgaaacga attaacgctc tgacgattgc
    12181 cggtactgat ccgagtggtg gtgcggggat tcaggccgat cttaaaacct tctcggcact
    12241 tggcgcttat ggttgctcag ttattactgc actggtggcg caaaataccc gtggcgtaca
    12301 gtcggtgtat cgcattgagc ctgattttgt cgccgcccag ctcgattcgg tgttcagcga
    12361 tgtgcgaatc gataccacta aaatcggtat gttggcggaa accgatattg ttgaagcggt
    12421 ggcagaacgg ttgcaacgtt atcagatcca aaacgtggta ctcgacaccg ttatgctggc
    12481 aaaaagcggc gacccgctgc tttcaccttc ggcggttgct acgctgcgca gtcgattatt
    12541 gccacaggtt tcattaataa cgccaaactt gcccgaagct gccgccttgc tcgacgcgcc
    12601 acacgcgcgc accgaacagg aaatgctgga acaagggcga tcgctgttgg cgatgggctg
    12661 tggcgcagtg ctaatgaaag gtggtcatct ggatgatgag caaagcccgg actggctgtt
    12721 tacccgcgag ggtgaacaac ggtttaccgc accgcgcatt atgaccaaaa acacccacgg
    12781 cactggttgt acactctctg cggcgttggc tgcactacgc ccgcgccata caaactgggc
    12841 tgacaccgta caggaggcaa aaatctggct ttcatcggcg ttagcccagg ccgacacgct
    12901 ggaagttggt cacggtattg gtccggttca ccacttccac gcctggtggt gagcaccgta
    12961 ctatagctgc tttggttatt gtcagccttt ggcactgaca ttcagggatg aaaccaatgc
    13021 agctaagaat taccagtcga aaaaaactca ccgctctatt atgcgctctt gggctaattt
    13081 ccatcgttgc aatttatccg cgtcagacgg tgaatttttt ctattcgaca gcagttcaga
    13141 ttacagacta catccacttc tacggttatc gcccggttaa atctttcgct attcgcattc
    13201 ctgccagtta caccattcac ggattagatg tttcgcgctg gcaggagcgt atcgactggc
    13261 agcgggtggc aaaaatgcgc gacaacgata tccgcttaca gtttgctttt attaaggcta
    13321 cggaaggcga aaagctggtg gacccctatt tttcgcgtaa ctggcaatta agccgcgaaa
    13381 atggcctgct gcgcggggcg tatcattatt tctccccgtc ggtatccgct tcagttcagg
    13441 cgcgattatt tctgcaaacg gtggatttct cacaaggcga tttccctgcc gtgctggacg
    13501 tagacgaacg gggaaaatta tcggcaaaag aattacgcaa gcgggtaagt cagtggctaa
    13561 aaatggtcga aaaacgcacg ggaaaaaagc cgattattta ctcaggagcc gttttttatc
    13621 acaccaatct ggcgggctat ttcaatgaat atccgtggtg ggtggctcac tattatcaac
    13681 gtcgtccgga caatgacggc atggcctggc gcttctggca gcattccgac cgtggacagg
    13741 tagatggcat caatggtccg gtggatttta atgtgtttaa tggcacgggg atgagttgca
    13801 ggcattcgtt gatgggatta aagaaacgcc ttaaataaga agcagtgata tactcccacc
    13861 agactccctg gcaggaaaaa gacaaatgga acaagcgcat acccagctga ttgcccaact
    13921 gaacgagcgt attttagcgg cggataacac gccgctttat attaagtttg ccgaaacggt
    13981 aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat attttgcccg gtgagcgtga
    14041 tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc aaggcgatgc aggcgctgga
    14101 agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg cagatcaaca acatcttcga
    14161 atactcgttg aaagaagcga gaggtttttc tcagcaggtg gtattgtgcg ggaaaaagcc
    14221 cgatacgcta tgggttaata agcgtgtcgt gaaatgtccc gaagaagttg cgcagcagct
    14281 ggcggtcgaa gcgggaagtg atgtcttttt gcttaagcgt attcgctatg tcgatgaaga
    14341 agcggtatcg attgaggaat cgtgggttcc ggcgcattta attcatgatg ttgatgccat
    14401 cggaatttct ctttatgact atttccgcag ccagcacatt tacccacaac gtacgcgttc
    14461 ccgcgttagc gcccggatgc cggatgccga gtttcagtca catattcagt tagatagcaa
    14521 aataccggtg ctggtgatca agcaagtggc gcttgaccaa cagcaacggc ctattgagta
    14581 cagcattagc cactgtcgca gcgatctata cgtttttgtg tgcgaggagt agttcctcgc
    14641 gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca cgacgccact gcattgccca
    14701 gtaatacggc atccgccagt ggcagaccag aggccagccc ggcaagtacg ccacctgcat
    14761 gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg aaatgccgga atgcagccag
    14821 aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag acgaacgatc aacggcgcgg
    14881 caaatttctc ctgccattgc ttgccaagtg ttgttatctc tgcggataaa gcaaaacgtt
    14941 cggcggcaat ctcagcctct tgacgattga gtgacactaa aggtcgacag gccatgatcc
    15001 gcgccagtaa tgcgtccggg atatcgccaa tacgtgggca aaaatcgata aacggcgtca
    15061 cgtcttgcag cttttccagc cattccacta acaattcgcc gcagggcgag gccagttgat
    15121 aaccggaaaa atagagcagg ttgccaggcg caacggttaa tcgtgccaac cattggcgat
    15181 tccattgatt ttccacaccg ctgaatgaca taaaagtgcg ttcgccatcc ggctcaacca
    15241 gtgccagaca ccagccgtta tcgctttcgg cgttatcgat caggctgatt aacccctctt
    15301 tcgccatccg gttgcgaata atctccgccc acacgccctg accgagcggc aaggcattac
    15361 ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt cagtgcgcag ccgccaacgt
    15421 taacgctctg ctgtttcagt tcgatatcgc acccacgcca ggggagggca taagcgtcgg
    15481 cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg ctgacgcgtg gttaattcag
    15541 gcagcagtgt gtgtaatcga gcgccgctca taccgcctcc cgttgttgac gatatttcgc
    15601 cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc gcatccagct ccgcctttaa
    15661 cgcaggatcg acagcgttaa tgccatgcaa cgcgccgcaa atcgccgtcg ccatagcacc
    15721 tatggtgtct gtgtcgccgc caagattggc gcacaggacg gcgcagcggt ttgggtcggt
    15781 ttgtgccagt tcaactagcg caatggcgca cggaacggac tcaatagtgc tggtgcctgc
    15841 gccaacgacc tggtaaagct gttcgctggc ggattcggtg ccgtcggcat tacgcacaat
    15901 tttcagcgca atctccagac gtgccgccag tgaggcgctg aacgtggtgg tgcgtttttg
    15961 ctgtgcatgt cgcgcaattg aaggcaatga atcaacaatc gctgaccagc gttctccgtc
    16021 aatggcacga gaaatcgccc atgcgatgac taccgcgcct gcaaccgcca gatcggattt
    16081 atgggtcggg ctggacgcca gcgccacatc atcaataaag gaatcaacat cacgcgctgg
    16141 aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg ttggttacgc cgttgttttc
    16201 cagttcagca acgggtttac cgtcgcgaat ggcgttaagc gcaatcttcg aggtcggacc
    16261 tagtacgttt ttgttaaagg cgtcgaaacg caacgcccag tcgagaatat tacgcccaat
    16321 cagattcgga tcgatcttgc cttcacattc cagtaacgca tccgccagac acaggccatc
    16381 gaggtatcgt cggtgaattc ggcgcggtta aaataacagg ccgcgttatt ctcctttggt
    16441 ccaggcagaa aacggtcaat ccagccaaag tgtgctttaa cgcggctgcg tggccaaagc
    16501 tcggagggca tccccatcgc atcccctaac gcctgcccat aaagagcacc gagaatacgt
    16561 tctgttttca tttaacttcc ccttgtgtca acgcaatatc tcgatcatcg accttgatag
    16621 ccgtaatttc gttgtcggat tcgcggaaaa aaatcatgaa cagcacggcg ataatggcaa
    16681 tcatcaccgc gccgaaagtc cacatcccgg accagttgaa agtcagtccg tttaccggtt
    16741 cctgataagc gaacatcttt tccatcatca caccgccaag acgatagccg agcaaactgc
    16801 cgaagccctg gcagcagagc gtgatcagcc cctgcgcagc ggtacgcata tgcacggggg
    16861 cttttttatc gacatagatg taagcggtaa cgtagtaaaa atcgtaactt acgccgtgaa
    16921 gcaaaatacc gaggaacagt aacgcgtagg tgaaatattc atccgcacta ccgtaaataa
    16981 agaagccata gcagatcgca gcggtgacca gaccaagcaa taataccttt ttgataccaa
    17041 agcgtttagt gaaaaacggc aatgccagca taaagaagat ttcagagaac tggccgagcg
    17101 tcatccagcc ggtggcgttt ttcatgccaa cttcggtcag ataaccgttg gcaaagatgt
    17161 aatagaacgc cagtggcatc gcaaacagga atgaacagaa gaaaaagacg aggaagtttt
    17221 tatcgcgcag caggatcagc gcatccaggc cgagcatgac tttaatgtcc attttgccgg
    17281 tgctttttgg cggcgtgtcg ggcaggaaaa acgcaaacac accgagcaga gcagaacttc
    17341 cggcggtaat cagcagcggg atgttagtcg gtgagatatc ggcatacccc agcatttgcg
    17401 gcaagaaacc acatgccaga ccggaggcaa tccagccgat agtgcccatc acacgaatgc
    17461 gtgggaaatc acgctccaca tccggcacgt tggcaaaagc gatgctgtta gtcagcgcaa
    17521 tggtcggcat ataggttagc gagtaggcca gcagtaacgg aaagaacccg gcaaaagtgg
    17581 tctgttgcgc agcgaaatac atcagcacag caccagcgaa catcaatacc gccagcactt
    17641 tctgcgccga gaaaaagcgg tcagtgatgg agccaaccag aattggcgac aggatcgccg
    17701 caatggctgt gcaggcatac gaccagccaa tttctccggc actaaaaccg cttttactta
    17761 accacaacca caatggcaca aaccacgcgc cccagataaa ccattcaaca aacatcatga
    17821 acgacagctt tgctgttgtt ttcatctttt aattccttca tgacaggtaa tgtaaggtac
    17881 gcgatgacaa taccacataa taatgccttt tgaatacctt tgcggtgaaa acttgaccgc
    17941 gctaacattt tttctgatga atcgagccaa cagaaaacgc tgaaaaaaca tccaaaagat
    18001 ggaaaaactc gcccgtaacg actaggatta tgattgcccg caaaaggcgg gccaggacaa
    18061 tccatagccg atatccaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt
    18121 tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc
    18181 agctttatct ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc
    18241 cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat
    18301 atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg
    18361 ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta
    18421 actgtgtggc atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca
    18481 ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc
    18541 aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga
    18601 ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg
    18661 aacgcgcgca cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct
    18721 ttaagaaaga tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc
    18781 tggcggcaac cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct
    18841 ataaagcgat taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa
    18901 acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga
    18961 taaactccgg cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg
    19021 ttatcaacaa acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat
    19081 cgatggctga cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa
    19141 ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt
    19201 tacactgcat ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat
    19261 agcactgcca cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc
    19321 ttccctatcg ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt
    19381 ttttgtgatc attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa
    19441 agcaaacgac aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca
    19501 cagcgcggcg gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg
    19561 gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca
    19621 tttactcatg ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat
    19681 caccatccac tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag
    19741 gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat
    19801 atttcacggg tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg
    19861 gagctggggc aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg
    19921 tacaccaacc ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg
    19981 gtcgccatcg gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga
    20041 ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta
    20101 tcgactaagg atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg
    20161 gagctgaaaa atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg
    20221 accgatcccc gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa
    20281 gtgattgccg attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa
    20341 aaacatgaaa acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt
    20401 ctgttctgcc catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg
    20461 caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat
    20521 gacaccggca gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg
    20581 cgaacgcatt attctcggcg gcgatcatct gggaccaaac tgctggcagc aagaaaatgc
    20641 ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag
    20701 taaaattcat cttgatgcgt caatgtcctg cgcgggggat accataccgt tagcaccaga
    20761 aacggttgcg gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg
    20821 ccagcgtgag caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc
    20881 cagcgccatt cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac
    20941 gcatcaaaag gcctttattg cccgtgggct gacagaggcg ttaacacgtg tgattgccat
    21001 cgtggtgcag ccgggtgtgg aatttgatca cagcaatatt atccattatc agccgcagga
    21061 agcgcaggcg ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac
    21121 cgattaccag acccggacgg cttattggga attagtccgc gatcactttg caatattgaa
    21181 agtcggtccc gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga
    21241 gcaggaactt atcgcccccg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat
    21301 gctcgacgaa ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg attcattact
    21361 ggatattcgt tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa
    21421 aaatagcgtt gaaacgatga tggtgaatct gcaaggcgtg gacatcccac tgggcatgat
    21481 tagtcagtat cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatgcc
    21541 gcatcagctt attatgaata aaatttatga tgttttgcgc gcctatcgct acggctgtgc
    21601 ggaataagga cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg
    21661 tagcgaagtt ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac
    21721 ctggccacag gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca
    21781 gcacgccatt gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct
    21841 gttaaggcca acaaataaag ttcattttca gcaagcggat gatgataacg acgtggcggt
    21901 atcgttggtt attgcgttga ttgtggaaaa tccgcagcag caattgaaac ttttacgctg
    21961 tttatttggc aagttacaac agcccgatat cgtcgagaca ctaattactc ttcctgaaac
    22021 tcagttaaag gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta
    22081 acaataaaaa gtactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc
    22141 gacctctacg atggcggcgg aagaaattaa agagttgtgt cagagtcata atattcctgt
    22201 tgaattaatc cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat
    22261 atgtaccact gccagggtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc
    22321 ttttgtttct ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg
    22381 gtgacctatg ttttcagaag tcatgcgtta tattctcgac ctcggcccta cagtgatgct
    22441 gccggttgtc atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa
    22501 agcgggtctg catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct
    22561 ggattccatt ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt
    22621 ggtcgatgtc ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt
    22681 ggcgattccg attgcgatat tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt
    22741 ggtaaatgtt gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcacct
    22801 ggcaaccggt tcatggatga tagggatggc gggtgtggta attcacgcgg cgtttgttta
    22861 taagctctgc gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc
    22921 tattccgcac ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat
    22981 cgagaaaatc ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt
    23041 tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct
    23101 cgcgggttac gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct
    23161 gctgatgcca cggatgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc
    23221 ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc
    23281 attactgctg gggcatacgg cggtggtatc ggcaagcctg atttttattc cgctcaccat
    23341 tttaattgct gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccactat
    23401 cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat
    23461 ctcgggtgtc atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca
    23521 cacccaaccg gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga
    23581 tcagggcggt tctcccatta cctggttact gattcaggtt ttctccccgc aaaatattcc
    23641 cggtttcatt attatcggcg caatttatct gaccggtatt ttcatgacct ggcgtagagc
    23701 gcgtggtttt actaaacaag agaaagccgt tctcgcagaa taatttttac ctgaggggga
    23761 ggttatcccc cttaacaatc aggagttttt atgaaatcag tagtgaatga tactgatggt
    23821 atcgtgcgcg ttgcaaaaag cgtcattcct gaaattaaac atcaggatga ggtgcgggta
    23881 aaaattgcca gctcgggctt atgtggttcc gatttaccca ggatatttaa aaatggtgca
    23941 cattattatc caataacgtt aggccatgaa tttagcggct atattgatgc ggtgggatcc
    24001 ggtgttgatg atttacaccc tggcgatgcg gttgcctgtg tgccgttatt accctgtttt
    24061 acttgtccag agtgtttgaa agggttttat tcccagtgcg caaaatatga ttttattggc
    24121 tcgcggcgtg atggtggatt tgctgaatat attgtcgtta agcgaaaaaa tgtctttgct
    24181 ctacccacgg atatgcctat tgaggatggg gcttttattg agccgattac cgttggcctg
    24241 catgcttttc atttagcgca aggttgtgag aataaaaacg ttattattat tggtgccgga
    24301 accattggcc tgctggctat tcagtgcgct gtcgcgctgg gagcaaagag tgtgacggcg
    24361 atcgacatta gctcagaaaa actggcactg gcaaaatctt tcggtgcgat gcaaacattt
    24421 aacagtagcg aaatgagcgc gccgcaaatg cagagcgttt tacgcgaact gcgctttaat
    24481 cagcttatcc tcgagacggc tggcgtaccg caaactgtcg aactggcggt agagattgcc
    24541 ggtcctcatg cccaactggc gctggtgggc acgttgcatc aggatctgca tttaacatcg
    24601 gcaacgtttg gcaaaatatt gcgtaaagag ctgacggtta tcggcagttg gatgaactat
    24661 tccagccctt ggccggggca ggagtgggaa acggcgagcc ggttgctgac agaacgtaag
    24721 ttaagcctgg agccattaat cgctcaccgt ggaagctttg aaagcttcac ccaggcggtg
    24781 cgtgacatcg ctcgtaatgc catgccgggc aaagtgttgc tcattccatg aaaccgcggg
    24841 ccagcgtgat gctggcccgg tattgtgcaa aacagatcat tcaccaatgg tcccccttcg
    24901 tttacactag ccacaattga atatgggtaa atgacgatga attcattcga gcgaaggaat
    24961 aagatcatcc aattagtgaa tgaacaggga accgtgcttg ttcaggatct ggcgggagta
    25021 tttgctgcct cggaagcgac aatccgtgcc gatttgcgct ttctcgaaca aaaaggcgtg
    25081 gttacgcgct ttcatggcgg tgcggcgaaa ataatgtccg gtaatagtga aaccgagacc
    25141 caggaagtcg ggtttaaaga gcgatttcag ctcgccagcg cgccaaaaaa cagaatagcg
    25201 caggcggcag tcaaaatgat ccatgaaggg atgaccgtta ttctcgacag cggaagtacc
    25261 acaatgctta tcgccgaagg attaatgacc gctaaaaata tcacggtgat taccaacagt
    25321 ctcccggcag cgtttgccct ttccgaaaat aaagacatta ctctggtcgt ctgtggtggc
    25381 acggtccgcc ataaaacgcg ctcgatgcat ggttccattg ccgagcgttc attgcaggat
    25441 attaatgccg atttaatgtt tgtcggtgct gatggtattg atgcggttaa tggtattacg
    25501 acctttaatg aaggttattc gattagcggg gcgatggtca ccgccgcaaa taaagtgatt
    25561 gctgttctcg attcatcgaa atttaatcgt cgcggtttta atcaggtact gccaatcgaa
    25621 aaaattgaca ttattgttac cgatgatgct gtgtcagagg tggataaatt ggcattgcag
    25681 aagacacggg taaaattaat tacggtgtag tattgttctg tatgccggat aaggtgttta
    25741 caccgcatcc ggcattacgg attctccctg gttcaagaga gaatagtttg attaacgcaa
    25801 taatggacaa tctggtggta atcgacaacg caacgccgcc ggaagtattt caatatggaa
    25861 gttttgccca ctcaacggtt cgccatcaag attaaaagtg atttcgtgtg gggcttgtat
    25921 atcaaaccac gacgaagcgc cttcgataat attcgggtta tcttcgtcag attttaaggt
    25981 tgatacgaga gccggaagaa tttcatcgcc ggtaaaaatg cgcagttgca gcaagccatc
    26041 gttaattaac gcgttcgggc acaattgttg accgccaccg gcctgacgcc cgttaccaat
    26101 accaatgacc agggcgtcac cttgccagtg aaagttttca ccgcggattt cacaacggtc
    26161 cggttgcaga gtatccatgc gcattaagcc atgaatgatg taagagacgc cacccagcgc
    26221 ggcttttaat ttttccggcg tttctgtggt aatacgcgtc ccaaatccgc ctgtcgccat
    26281 attaataaaa caggtttgtt tgttgacctg cgccatatct atcgcaatgg cattaccggc
    26341 aattgccagt ttcagcgcct tatccagtgc ctcaggaatc cctacactgg tggcaaaatc
    26401 attggcggtt cctaatggca aaattcccag cgcgggtatg tcatccccct cacactgaat
    26461 caacgccgta gaaacttcat taatggtgcc atcgccacca ccggcaatca ccgttgcgac
    26521 gcccaacttc cgggcctcct ctacaaatcg tgcggcatca cctttctccc aggtgacccg
    26581 cacatggatc gtcattcctt cctcacgcaa cagcataatt gcttcgcgca agggtagatt
    26641 gtcagtactt ttaccattaa gaatcagtaa gctggcggga aattctgcca tgatcgtatg
    26701 tgcctttatg attggtctgt agatagtgta gagcagaaaa caaaaggtgg agtcagaaca
    26761 ggatgaaagt cggaggataa aaagtcagtt caagtatgga ggattcagtg gttaccacca
    26821 ggagctttca atggggacag cgaatactaa cggctgccac cgataaattt caaaaaagag
    26881 catataccta atattcaact aaacagtggc atcttcaata taatatatta aagcccccat
    26941 ggagttaccc tgaagggcct caatgtccgt aattcctact tatgtaggaa atgttgtaca
    27001 gagcatttat tataatccta ttcaattata ataatcatgt cattattata tttaaacact
    27061 agagagtgtc gttggtattt aatgggggaa ggtgagatga aaaagatagc tgctatatca
    27121 ttaattagta tttttcttat atctggttgt gctgtgcata atgatgaaac aagtatcggt
    27181 aaatttggtc ttgcatataa aagtaatatt cagcgtaaac tcgataacca atactacacc
    27241 gaagccgaag cttctttagc caggggcaga atatctggtg cagaaaatat agtaaaaaat
    27301 gatgcagccc atttctgtgt tactcagggc aaaaaaatgc agatagttga cctgaagaca
    27361 gaaggtgcag gattacatgg cgtcgctcgt ctgacattca aatgtggaga gtgagaatat
    27421 ttttttgtaa gcgtcaaaca tgcgcgttct ggctgtgcgt agccggaacc tgggcgagca
    27481 cgataccgtt acgtgaaagg cattgtgcta tgaaggggga ttctatcgat gtggtcaatg
    27541 gaagacggtt atcagggatc aatccttctt tacagcttat gtacaaaaaa taagcccgtg
    27601 taagggagat tacacaggct aaggaggtgg ttcctggtac agctagcatt ttatggatta
    27661 tgtttttcag cgaaacggat gataacctta ataaatgcag ctgtatgtga tcggtttcta
    27721 agaattttcc atccgggaaa aataatcgaa attaatcact taccgtgggg gttacgcgtg
    27781 gtttccccgg agaaattacg cattagcagc gcgtaattta gctcaagatc ctgcgggacc
    27841 gggagccaca cagtataacc atcgcctggt gcgaccggca tagcttcgcc tttggcgttt
    27901 tccatgtgct caagggtaaa gttaatgttg ccttgcggcg tcatcagctc aagactgtcg
    27961 ccaacggaga atttattttt caccgctacc gctgcgaggt cccccttgcg ctcaccggta
    28021 aactcaccaa caaactgctg gcggtcagaa actgaataac cgtattcgta gttctgataa
    28081 tcgtcgtgag tatgacgacg caggaaacct tcggtatagc cacgatgcgc cagaccttcc
    28141 agagtttcca gcaggctggt atcgaacggt tttcccgcag cggcgtcatc gatagctttg
    28201 cggtaaacct gtgcggtgcg tgcgcaatag tagaaagatt tggtacgacc ttcgattttc
    28261 agcgaatgca cgcccatttt ggtcaggcgt tctacatggg cgatggcgcg cagatctttc
    28321 gagttcatga tgtaagtgcc gtgctcatct tcaaacgcgg tcatatactc gcccggacgc
    28381 tgggcttctt cgatcataaa cactttgtcg gttggcgcgc cgatacccag cgtcggctca
    28441 acattttgca ccggaatcgg ctcgtacttg tgtacgatgt tgccaacgtc atcttctttc
    28501 ccttcctgga cgttgtactc ccagcggcag gcgttggtgc aggtgccctg gttcgggtcg
    28561 cgcttgttga tatagccaga gagcaggcag cgaccggagt aggccatgca cagtgcgccg
    28621 tgaacgaaga tctcgatctc catatccggc acctgattat ggatctcttc aatctcttcc
    28681 agcgacagct cgcgagagag gatcacgcgg gtcagtccca tttgctgcca gaatttcacc
    28741 gtcgcccagt tcacggcgtt agcctgcacc gagaggtgga tcggcatttc agggaagtgc
    28801 tcacgcacca gcataatcag ccctggatcg gacataatca gcgcatccgg ccccatttcc
    28861 accaccggtt tcaggtcacg gataaaggtt ttcagcttgg cgttgtgcgg tgcaatgttg
    28921 accacgacat aaaacttttt ccccagcgcg tgggcttcat tgatgccgag ctgaagattt
    28981 tcgtggttga attcgttgtt gcgcacacgt agggagtaac gcggctggcc cgcataaaca
    29041 gcatctgcgc cataagcgaa agcgtaacgc atatttttca gcgttcccgc cggggaaagg
    29101 agttccggtt taaacataat tctctcgttc tgatgacagg tcagatccgc ttcacctgat
    29161 gaagcggtta ggggaggccc ccactttaag ggcgggcatt gtagcgctgt aggggggatc
    29221 tggtaaagcg ccggacgcca acaacccacg cccggcatcg taattacgta ttgtctttca
    29281 ccgtctggct tgtgccattg gctttaacgg aggcaattcc ggtttcacga gattgcgcgg
    29341 tggcgtacat ctggctggag ccgataattt gatgattagc ggctttcaga ttgaaataga
    29401 atttgccgtt gcttgccgtt ttttctcata gcgctcttct tgcgggctgt tgctacgcac
    29461 cgacgcgatg cccttttccg cagaggcttt tgaggtatac agctcgctgg tgaggatagt
    29521 ctcaccattg cccgctttta gcacaaaccg gaactgatta tcactgctct tactgagttc
    29581 aaaccaacca gccataatga ctccttgatt gaaattgctt atcgaaaaca gcatctaa
//
LOCUS       DAJVRP010000048        29080 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVRP010000048 DAJVRP010000000
VERSION     DAJVRP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.048"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(173..1069)
                     /gene="yhaJ"
                     /locus_tag="OQN12_002141"
     CDS             complement(173..1069)
                     /gene="yhaJ"
                     /locus_tag="OQN12_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCS1654454.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1174..1875
                     /locus_tag="OQN12_002142"
     CDS             1174..1875
                     /locus_tag="OQN12_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1654455.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1898..2062
                     /locus_tag="OQN12_002143"
     CDS             1898..2062
                     /locus_tag="OQN12_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654456.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2274..3584)
                     /locus_tag="OQN12_002144"
     CDS             complement(2274..3584)
                     /locus_tag="OQN12_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1654457.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3612..4943)
                     /locus_tag="OQN12_002145"
     CDS             complement(3612..4943)
                     /locus_tag="OQN12_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS1654458.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5218..6582)
                     /gene="tdcG"
                     /locus_tag="OQN12_002146"
     CDS             complement(5218..6582)
                     /gene="tdcG"
                     /locus_tag="OQN12_002146"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1654459.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6654..7043)
                     /locus_tag="OQN12_002147"
     CDS             complement(6654..7043)
                     /locus_tag="OQN12_002147"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1654460.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7057..9351)
                     /gene="tdcE"
                     /locus_tag="OQN12_002148"
     CDS             complement(7057..9351)
                     /gene="tdcE"
                     /locus_tag="OQN12_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS1654461.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9385..10593)
                     /gene="tdcD"
                     /locus_tag="OQN12_002149"
     CDS             complement(9385..10593)
                     /gene="tdcD"
                     /locus_tag="OQN12_002149"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1654462.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10619..11950)
                     /gene="tdcC"
                     /locus_tag="OQN12_002150"
     CDS             complement(10619..11950)
                     /gene="tdcC"
                     /locus_tag="OQN12_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1654463.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11972..12961)
                     /gene="tdcB"
                     /locus_tag="OQN12_002151"
     CDS             complement(11972..12961)
                     /gene="tdcB"
                     /locus_tag="OQN12_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1654464.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13060..13998)
                     /gene="tdcA"
                     /locus_tag="OQN12_002152"
     CDS             complement(13060..13998)
                     /gene="tdcA"
                     /locus_tag="OQN12_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1654465.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14188..14532
                     /gene="tdcR"
                     /locus_tag="OQN12_002153"
     CDS             14188..14532
                     /gene="tdcR"
                     /locus_tag="OQN12_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1654466.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14788..15327
                     /locus_tag="OQN12_002154"
     CDS             14788..15327
                     /locus_tag="OQN12_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654467.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15349..15696
                     /locus_tag="OQN12_002155"
     CDS             15349..15696
                     /locus_tag="OQN12_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654468.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15865..16515
                     /locus_tag="OQN12_002156"
     CDS             15865..16515
                     /locus_tag="OQN12_002156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654469.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(17023..17399)
                     /gene="rnpB"
                     /locus_tag="OQN12_002157"
     ncRNA           complement(17023..17399)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQN12_002157"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17432..18577)
                     /gene="garK"
                     /locus_tag="OQN12_002158"
                     /pseudo
     CDS             complement(17432..18577)
                     /gene="garK"
                     /locus_tag="OQN12_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(18674..19564)
                     /gene="garR"
                     /locus_tag="OQN12_002159"
     CDS             complement(18674..19564)
                     /gene="garR"
                     /locus_tag="OQN12_002159"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1654470.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(19594..20364)
                     /gene="garL"
                     /locus_tag="OQN12_002160"
                     /pseudo
     CDS             complement(19594..20364)
                     /gene="garL"
                     /locus_tag="OQN12_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(20380..21714)
                     /gene="garP"
                     /locus_tag="OQN12_002161"
     CDS             complement(20380..21714)
                     /gene="garP"
                     /locus_tag="OQN12_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1654471.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            22089..23660
                     /gene="garD"
                     /locus_tag="OQN12_002162"
     CDS             22089..23660
                     /gene="garD"
                     /locus_tag="OQN12_002162"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCS1654472.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(23712..24536)
                     /gene="agaR"
                     /locus_tag="OQN12_002163"
     CDS             complement(23712..24536)
                     /gene="agaR"
                     /locus_tag="OQN12_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCS1654473.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            24785..26065
                     /gene="kbaZ"
                     /locus_tag="OQN12_002164"
     CDS             24785..26065
                     /gene="kbaZ"
                     /locus_tag="OQN12_002164"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCS1654474.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            26088..26561
                     /gene="agaV"
                     /locus_tag="OQN12_002165"
     CDS             26088..26561
                     /gene="agaV"
                     /locus_tag="OQN12_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCS1654475.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            26572..27351
                     /locus_tag="OQN12_002166"
     CDS             26572..27351
                     /locus_tag="OQN12_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCS1654476.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            27341..28150
                     /locus_tag="OQN12_002167"
     CDS             27341..28150
                     /locus_tag="OQN12_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCS1654477.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            28237..28671
                     /locus_tag="OQN12_002168"
     CDS             28237..28671
                     /locus_tag="OQN12_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1654478.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            28668..>29080
                     /locus_tag="OQN12_002169"
     CDS             28668..>29080
                     /locus_tag="OQN12_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1654479.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         7564 a         7592 c         6865 g         7059 t
ORIGIN      
        1 ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc agcctacaaa
       61 ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag cgcatcaggc
      121 agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata gattattttc
      181 cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc atactgtcac
      241 gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg acacgcaacc
      301 gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg ccaagccccg
      361 ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc tgtttgtcca
      421 gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca attccacgat
      481 atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg tgatcaggcg
      541 cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc gacgaggaac
      601 gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc cgttcccacg
      661 cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc gccagtttgt
      721 cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg gtgagatgcg
      781 tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg gcttccagca
      841 aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg cgatggcccg
      901 agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatggtg tagctaagtg
      961 cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg cgatcgatcg
     1021 catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt tcgttctcat
     1081 tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt ccagcccctt
     1141 accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac tgccaggcag
     1201 tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt tggacactac
     1261 ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga agtgctggcc
     1321 ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa tgtgattctg
     1381 gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag cgccggagag
     1441 gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct cagcaaagac
     1501 aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga gaatccgctg
     1561 attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc agagggtgcg
     1621 atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct cgacaaaggc
     1681 gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat tcacgggaaa
     1741 tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga tggggcgttt
     1801 attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc tttgctgata
     1861 gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat cgaccaaaaa
     1921 gcgccagcca gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca aaaattttgg
     1981 ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa cgcgtttagc
     2041 cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg ccttatccgg
     2101 cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata agatgcgcca
     2161 gcatcgtatc cggcatcagg tacaaaacac aacaacatca acaagttgag caccccgtag
     2221 gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg cgtttatctg
     2281 gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga atggcttgct
     2341 aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc ttcattgccg
     2401 gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga agcactggtc
     2461 gaaaccttca tcgcgcagct gttcgacgca ccatcgcaaa tcatgccgct gacatcgccg
     2521 atcatactgc tgatcgccat cgagatagtt tcataacgcc catccaccag ccatgccatc
     2581 ccggcggcgg cccccattgc tgcggtcgtt gcggcacaaa gtgcagacaa acgcggtaac
     2641 tggttatgga tgtaaattgc gctcaaatga gaaagcatca gcgcacgcgc cagtcgttca
     2701 tcatccgctc cgaagtgttc tgctacaacc accacaggca ttattgcagt gatcccctgg
     2761 ttacccgagc cggagttact catagccgga agcgtagcgc cgcccatacg cgcatcggat
     2821 gccgcgctgg tacgaatcac aatggatgaa gagagatctt tcgccagcaa accgcgctcg
     2881 cactgttttt ccagcgtcgc gccaatatgc agcccccact taccgctcaa accttcctgc
     2941 gataacgcac aatttagctt cgcggaatcg agaataaagc ggatcgccgc aaatgggact
     3001 tcattgacga acttcaggat ctcagccagc gtcgttctgg aaagcaccgt cagcggcgac
     3061 tcttgctcgc cctctgccac acacgcctgg tgggtaaaca ccacaccatt gtgcgtctca
     3121 atatgcacaa tgttggtatg cccgccgacg atggtgacac acgcccactt ctcaccgttc
     3181 cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt cctggatctt aacagagact
     3241 ttccccgccg ccagcagtgc tttggcatcg gcaattgcct gcgcagttgc gtctttcagc
     3301 acttccagcc cggcgttggc atttccacct aacgccccca gcgccgccgc aatcggcagc
     3361 cccaccattc ccgtgccggg aacggtgacg cccagaccgt tcttcatcag atttggcgaa
     3421 acccaggctt ctacacgttc aaccggacct tccagttctg ccgcagcaac cgccgccgcc
     3481 agcgccagtg aaatcggttc agtacatccc agcgccggtt ttacttcctc ctgaacggcg
     3541 aggatgtaac gctgccataa cggatttaaa gtcgaatcaa acatattaaa aaccttaaaa
     3601 tttcaggtaa atcaagaaaa tgccaggaac agagaaacac aaagcaacaa accagtgacg
     3661 ataatcaggt acagagacat ccctttgtat ttgtgcaatg ccggtacttt gtaaaccagc
     3721 cacgccggga tcaggcaccc taccatgccg aaaatcgggc tacagataga ggtgaagctc
     3781 aacaccggtg cgttcagtac gatggcgctc caggccagca aaatggcgaa aatcatgatg
     3841 ccgcgctgaa caagattttc gttaatcttc tcggcaggca tcttgcgacg caggatgttc
     3901 attacgatcc cttgcgttgc ttcgcgaaag cctaaataga cgccaaagaa tgcagtcatg
     3961 acagcaaaga tattgagaat gacgctgacc actttcaccc acgctgcgcc gtcaccgcta
     4021 ataaactgtg cggcaatcgc cagcgcggaa atattctgct cgtaggcttt taccgcttcg
     4081 tcatgtccca tcgccagcgt gaacgacacg gcgtagaaaa agacggtgac aaacaaaatg
     4141 ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg ccacttcaat tgatttttcc
     4201 cgcgagcgat aagagatcac catcgggctt aacgtctgga taaacagaat cgacgtcagg
     4261 gtaaacggca gcgtaataat ggcgtttttc accagcagcc ccagcggcgg tagcgaaccg
     4321 acgttataca gatgccacat tcccaccatc gacacgccca gcgcagcgac taccagcagc
     4381 ttggtcagca ccatgtcggt cgaaattttg aataacaatt tctcgccgcg tgaagagatc
     4441 gccaccagaa tgcaaatcag taccagacca taaaagggac tgtctgacag caacccttcc
     4501 gtcacgccga aggtatgcag ataggaagca ctatcgttgg tgatggcggt ggaatagacg
     4561 aacatccaaa tcaccagcat tacgaaatag agcgcaccta acaggatgcc ccagttttta
     4621 cctaaataac cgctaatgac actcgggtaa tctttacatt ctggtgattc tgccagcgta
     4681 ttaataaaca accgctgaaa cagatacatt gccgggtaac caatcaccga tgagagcaaa
     4741 aatacccaca atcccatcaa accgacctgc accgggagaa aaacaatccc cgcgccaatc
     4801 gccatcccga tactcataat cacccagccg gtgtcagtac tgtcgaattt gatcgcttct
     4861 cgccactcgc tctcactcat tccagcacga cccgccgggg tcgaagcgtc tgcaatgacg
     4921 cctttattcg atgcaatttc cataatttct cgctcaatat tttgtagggc ttattatttt
     4981 tttccgagcc gcatcaaggc gatatgcggt ctacgttttt gcaggcgagt tattagaata
     5041 aagaaatgat acgcgcgagg tcagagaaaa tcttcacaat caagacttta aaataacgga
     5101 gatggataag aattttctac taaattaatc gcaagagaat attcattctc tatttgcggc
     5161 agatcacaaa aaaaaggtgc acatttgtgc acccaaggat gaaagctgac agcaatgtca
     5221 gccgcagacc actttaatgg ccagtcctcc gcgtgatgtt tcgcggtatt tatcgttcat
     5281 atctttgccg gtttcataca tcgtctcgat cactttatcg agtgaaacac gcggtgccga
     5341 ggtgcggcgc atcgccatcc gcgcggcgtt tactgctttc acggcattaa tggcattacg
     5401 ttcaatgcac gggatttgta cctgtccggc aaccggatcg caggtcagcc caaggttatg
     5461 ctccatcgcg atttccgccg cattgcatac ctgcgccgga ctgccgccca gtagttcagt
     5521 taaccctgcc gctgccattg aacaggccac gccaatctcc ccctgacagc cgacctccgc
     5581 gccagagatg gaggcgttca ttttatacag cgcgccaata gcccccgctg ccagaaaata
     5641 gcgggcaatt gaccgctcgt ttaccggacg acggaactta tcgtaataag ccagcactgc
     5701 cggaataatg ccgcacgcac cgttagtcgg tgccgttacc acgcgcccgc cagctgcgtt
     5761 ttcttcacta accgccagcg cgtacatgtt gatccagtcg atgacattca tcggatcgtt
     5821 agagatgtta tcgctagaaa ccagctgacg acgcagcgct acggcacgac acggcacatt
     5881 gagcggacca ggcagcacgc cttcagtgtt catcccgcgt tcaataccgt catgcatcac
     5941 ttgccagata cgggcaaacc ctgcgtcaat ttccgctttg ctgcgcagcg ccagctcgtt
     6001 gtgcatcatc aggccagata tagacaggcc gttgtaatca cacattttca gcagttcacc
     6061 tgctgagtgg aaatcgtaag gtacggacgt ttcgacatcg tgcgacaggc cgaagtgttc
     6121 ttcttcgaca ataaacccgc cgccgacaga gtaataggtt ttacttaata gcgcttcctg
     6181 tcccttccag gcagtgatcc gcattccgtt ctcatggcga ggcaacattt cgggatggaa
     6241 gataatgttc tttgctacag gaaaatcaac aatatgcgca ccagatgcca ctggcagccg
     6301 cccgctgcgc gttactaact ctataaatgc agggatctca tcaatgacaa catcctgcgg
     6361 actgtttcct gccagtccca tgataatggc gacatccgtg gcatggcctt tgcccgtcag
     6421 tgacaacgac ccgtacagat cgaccacaat atggctcgtc gcggttaata agccgctact
     6481 ttccagccga tcaataaaac tttttccggc attcattggc cccacggtat gcgaactgga
     6541 gggaccaatc ccaattttga aaatatcgaa tgcactaatc atatccacac cctcggattg
     6601 ccgttcagtg aagtggagcg gaacgacctt gcgaccgtcc cgctcacgag gctttacgca
     6661 ctacgtactg cgatagcttc aatttccagc ttcacatctt tcggcaaacg cgcaacctgc
     6721 acacagctcc gggtcgggta ggtcgcctga tgctcatcga agaactgctt atagacttca
     6781 ttgatggtgg caaaatcatt cagatcggtg atgaacacgg tcatcttgat gatatcgccc
     6841 acgctcagcc cggcagcaac cacgatcgct ttgacgtttt cgaggcttaa acgtgcctga
     6901 tcttgcacat cagccgggat ctcaccggtc tgcgggcaaa ccggaatttg cccggaggtg
     6961 aagaccatgc tgcctaaatc aacgccctga acataagggc cgattgcgcc tggggcacgt
     7021 tgcgtttcga taatcttttt catacatcct ccggcgtcag agcgcctggg taaaagtacg
     7081 tgaaataaca tcctgttgct gttcacgggt cagtgcgttg aagcgcacgg cgtagccaga
     7141 gacacggatt gtcaggttag gatatttttc cgggtgctcg atggcatcca gcagcatttc
     7201 ccgattcatt acgttgacgt tgaggtgttg accgccttcg acatccgctt cgtggtggaa
     7261 atacccatcc agcaggccaa caaggttggt tttacgtact ggatcttctt tgcccagcgc
     7321 cgcaggaacg attgagaagg tgtacgagat cccatctttg gcgtaggtga acggcagttt
     7381 cgccaccgac gtcaatgagg ccacggcacc tttgcggtca cgaccatgca tcgggttagc
     7441 gcccggcgcg aacggtgttc cggcgcgacg accgtccggc gtattaccgg ttttctggcc
     7501 gtacaccacg ttagaagtga tagtcagaat cgactgggta gggacggcgt tgcgataggt
     7561 tggcagcgct ttaattttct tcataaagcg ttcaaccagg tcgcaggcaa tgctgtctac
     7621 gcgctcgtcg ttgttgccgt actgcggata ttcaccgtcg atttcaaagt ccaccgccag
     7681 gccgttttcg tcacggattg gtttcacgcg ggcatatttg atggcagaca gggagtccgt
     7741 cgccaccgac aggcccgcga tgccgcatgc catagtgcga tagacatcac gatcgtgcag
     7801 cgccatcagc gaagcttcgt agctgtactt gtcgtgcatg tagtgaatga tattcagcgc
     7861 gctgatgtac tgcaccgcca gccagtccat gaagtgatcg aggctgtcca tcactttgtc
     7921 gtaatccagc acgtcgtcca tcagcggtgc tgttttcggc ccgacctgaa tcttcagctt
     7981 ctcgtccacc ccgccgttaa ttgcgtagag cagcgttttc gccaggttag cgcgtgcacc
     8041 aaagaactgc atttgcttac caatcaccat tgggctgacg cagcaggcaa tcgcgtaatc
     8101 gtcgctgttg aagtcagtac gcatcagatc gtcattttca tactgcaagg aagaggtgac
     8161 gatcgacacc tgcgcggcat attttttgaa ggcaatcggt aattcttccg accaaagaat
     8221 ggtcaggtta ggttccggtg ccggccccat agtgtgcagg gtgtgcaaat agcggaagga
     8281 gtttttggtc accagcgtac gaccgtccag ccccatcccg ccgatcactt ccgtcgccca
     8341 gattgggtcg ccggagaaca gcgaatcaaa ttccggtgta cgcaggaagc gtaccatacg
     8401 gatcttcatg atgaagtgat cgatcagttc ctgtgcctgc tgctcattga ggacgccagc
     8461 tttaaagtcg cgctcaatgt agatatccag gaacgatgcc gtgcgcccca gcgacatcgc
     8521 gccgccattt tgcgatttca ctgccgccag ataagcgaag tagagccact gcaccgcttc
     8581 ctgtgcattc tgcgccgggc gagagatatc aaagccatat ttcgccgcca tttcctgaat
     8641 ctgcaacagc gcatgacgat gctctgccag ctcctcacgc agacggatgg tggcttccag
     8701 atcctcgcct ttttccagac gagactggag atcggcaaat tgcagttcgc gttcacgcac
     8761 cagataactg atgccataca gcgctacgcg gcgatagtca ccgataatgc gcccacgacc
     8821 atagccatct ggtaaaccgg tcagcacgcc agatttacgg cagcgcagca tatccggtga
     8881 gtaaacatca aatacgccct ggttatgggt tttacgcaga tcggtaaaca gatattcaaa
     8941 ttcactgtcc atttcacggc cataggcgtg gaatgaactt ttaatcatat taatgccacc
     9001 gaacgggtgt agcgcacgtt tcaacggcgc atccgtttgc aggccaacaa ttttttccag
     9061 cggctggtta atatatcccg catcatgagc ggtaattgtg gtggcaatat tggtatcgaa
     9121 atcaaccggc gcgtgggttg cgttttcgat acggatgcct tccattacct tttcccacaa
     9181 ttccgtggtg gcaggcgtcg cttcggcgag aaaagattca tcgccttcat acggtgtata
     9241 gttatgttga ataaaatcgc ggacattaat ttcgcttttc cagtccgtac ctttaaagcc
     9301 aagccatgcg tcagcgtaca gcttatcgct ggtatcaata tctaccttca tgaaaaataa
     9361 tctctctaca atacttcaac taaattatgc aaattctgcg ggcgcgttaa ctttacctaa
     9421 atgaatggca tccaaagcaa tcattttttc ttcgttagtc ggaataacga cgcaaatgac
     9481 atgcgcattt tcactggaaa caattcgctc gccaaaagag ttagatcgat tattcatttc
     9541 tgtatcaatc tctacgccta atacagccaa atgttccatg accagacgac gaattaaact
     9601 tgaattctct cctattccgc cggtgaatat aattccatcc agccgacgta atgaagctgc
     9661 atgtccggca atatgacggg caattcggtg aacaaaggtt ttaattgcca gttgcgcgcg
     9721 ttcgtgacct tcatgccagg ctttttccag aacacgtaaa tccgaagaaa gaccggaaat
     9781 acctaataat cccgactctt tattcactac gcgttccagg tcacccaggc tctggttggt
     9841 ttggctggcg acccaggaca tcgcaccaaa gtcgacatcg ccactgcggg tacccatcat
     9901 caagccttcc agcggcgtca ttcccattga ggtgtcaaca ctctgaccgt tgcgaactgc
     9961 gcagattgac gcgccattgc caagatgcgc cacaaccagg ccggagtcat cttccgccag
    10021 attcagcagc gaatgtgcgc gctgggaaac atagcggtgc gacgtgccgt ggaaaccata
    10081 acggcgtaca cctaactctt cataatattt ccacggcagg ccgtataaat aagcttccgg
    10141 agccatcgtc tggtggaaac tggtatcaaa taccgccacc tgagttacgc ccggaaataa
    10201 ttgctgcgcc gattcaatac cacttaaatt tgcgtaatta tgcaggggtg ccagtggaga
    10261 aacgcgacgg atattatcaa tgacttcatc ggtaataatg gcggactcgg taaaaatact
    10321 gccgccgtga gcgatgcggt ggccaattaa ggccacgctg tcatttaaat tccgtttttc
    10381 cagttcaaat gcaattgcct tcaatgcacc ttcgtagctg tggtgagcca gcggtgctgg
    10441 ctctccccca tttacggata agaatgcatt ttccgagtta ataccgtcgg caatacctga
    10501 cattaatact tcacaatcgc tggcatcgag tacggaaaac ttaatcgaag acgaaccaca
    10561 gttaataacc aaaacaaccg gaaattcatt catctctttt ctcatcctga gttacggatt
    10621 aaaacagttt gtatacgatg ttcaggatgg tcagcagacc aatcacggta acaaacacgt
    10681 tatccagacg accacggtat ttcgccagag acggcgcttt acggatagca tacatcggca
    10741 acaggcacag cagggatgcg ataatcggtg cgcccatggc ttcaatcagg tcgaggatgt
    10801 tcgggttggc gtaggcaaca acccaggtgg agcccatgat gaagatcatg ctgagagtat
    10861 tcagtttacc cagcgacact ttggttttgt cacctttata accgaacttc agaatcagac
    10921 cattcaagcc ttccagcgtc cccagatagt gaccgaagaa agatttgaag atagccacga
    10981 gtgcgatgat ggaagccgca tattccagtg taatcgcgaa cgttgttttc gtaccggtca
    11041 tggacgcaaa gtggttagcc agataagaaa gcactggaat attctgcgct ttggcttccg
    11101 ccatgttggc cggagacaga gtaaacaggc agctaaaggc aaagaacatc accactgcaa
    11161 ccatcagcat gctggcacga gaaatgattt gggaacattt acgttcggtg aagtcgcgac
    11221 cgaagtcttt ctcatactct tcacgtttag aaaccacgaa ggaagagacg attggcgaga
    11281 agttaaagga gaaaaccatg atggaaatcc ccagccagac agtgatcagg ataccgtcat
    11341 gaccggttaa cgacagcgaa ccgaggtcaa cctggtcgat aactgcagag ttccagtaag
    11401 ggatcagcga cagagaaatc agcaccaggc tggcgataaa cggccatacc aggtagctca
    11461 tcactttaac catcagatcc ttaccaaacc agatgacgaa agccatcagc agcaacagga
    11521 acagcgccac aaagccgcga ttcagcggtg caaatccgag ctggttttcc cagaacgtca
    11581 taaaggtatt ggtaatagta acgccataaa tccacagcag tgggcaaatc gcgaagaagt
    11641 acaggaacgt gataaccacg ccgccagttt taccaaaatg ctcttccacc gtttccgtaa
    11701 tgttgccgga agggttagag ccagaaagac acagacgcgc cagcgcccgg tggcaataaa
    11761 atgcgatggg gtatgccaat accaacatca gaagaatcgg gatcagtccg ccaaaacctg
    11821 cgcggatagg gaagaacagc accccggcgc cgattgccgt accaaacaag cctaacgtcc
    11881 atgtggtatc tgatttacgc caggacgatt gttttgtctg gctggataca atgctatctg
    11941 aagtactcat atcctatcct caacgaatta attaagcgtc aacgaaaccg gtgatttgag
    12001 agacgcgaga aagatcgata ttgccgccgg aaataatact gacggttttt ctgttttgaa
    12061 tatattggtc taatttaccg cttaataatg cagcacatgc cagagcgcct gcgccttcgg
    12121 tgacgacttt attgcgctga attaaggcaa tcatactgtt tctgatttcg tcttcgctga
    12181 ccagcacgat gtcatcgact aattcacgaa cgatttcgta agttaaatta cccgggcggg
    12241 agacatcaca accatccgcc agggtgccgg tagttcggtg cgtggttatt tctccggagt
    12301 ggaaagaagc cgccatgccg tgaacgtttt ctgactgtac gccaataaca cgaatggtcg
    12361 ggttaataga tttaattgcc accgcaatac cagcaattaa accaccacca ccaattggca
    12421 caatcacgtt atcgacatca tagagatctt ccataatttc cagaccaatc gttccctggc
    12481 cagcaatcac tttcggatca tcgtaaggtg ggataaaaat acgaccttcc atttcgacaa
    12541 tttcgctcac tttagcgata gtgtcgttga agttatcacc atgcagaacg acttctgcgg
    12601 agtagtcgca cgttgccgct actttggatt ttggcgcacc ttttggcatc accactttac
    12661 cgtcgatacc cagcatcgcg caggagagcg aaaccccttg cgcatggttg cccgcagaac
    12721 aggccaccac gcctttgcgt ttttccgcat cggtcagtga acttaattta ttaaatgcgc
    12781 cacgaatttt aaatgaaccc gtacgctgca tattttcaaa cttcaggaat atttcacctt
    12841 tgcaacgttc actaaaatag ttggagcgag gcatgcctgt tttataaatt cgcccagcca
    12901 gtcgttgttt cgcttcaata atatcatcaa tagcaaccgg cagatcgtat gtaatatgca
    12961 ttataaaacc tcttcgcctg aattaaatat gtaggtaacc gtaaccgaca cctgcaaaac
    13021 agacaggtgg attatttatg tttaaataaa aacaaacaac taaccaactt ctattaattg
    13081 ccttcgtcta cacccattat aagatgaata ctctttggct aattccacca aaaccgatgc
    13141 tgctttttta atacgataat ttttcgacca taccgcggca tattgtgcca caggtaatgt
    13201 ttcttcaacc ggaatagtaa taaattgatt agaaccaaaa ggtgacgtca tatcacaagg
    13261 aattacagtt aagaaatcag cattgagaac aagattataa attgtcacga ctgagtcggt
    13321 tttaacgatg ttttcaatac tgatgccatt tctttgtaac gtagtaagca gttcgctgta
    13381 gtaccccata ttagtttgtg gcaacaccca ctgttcgttc ttcaacgact ccagcgtggt
    13441 ggtgccggtg catgttcggg acttactggc taccagcaca aactcggact cgaacagcgg
    13501 ctcaacatgt aaatcctgaa gcttcatttc tgcacttaac gtaccaatcg caaaatccag
    13561 tcgaccgtcg cggattgccg gtaagaacga agacagttgc gcttcataca tagaaacctg
    13621 cgctttcggg aacacctctt tgaacttgtt gatcatccct gacataaaag taaaaccaat
    13681 caatgaagga aaaccaaatg agacttccac caccgcttca gaagacatac cgcttatctc
    13741 attaaccata tttttcattt cacgggtaat ggattcggaa cgggagagta acaattgacc
    13801 ggcaggtgtt aatgttacac cggtattttt ccgcaccact aattccacac caaaataatc
    13861 ttcaatatcg ttaatgattt tactgacggc cggttgagtt aaccctaatt cttttgcagc
    13921 cgagccgata gagccacttc taatgacttc ctgaaagact accaggtgct gcgttttcgg
    13981 aagaagaata gtgctcataa cgacctacgt taattacctc attgacggca tgaagtgtat
    14041 caaaatgaaa taaacaggat atgtgcgacc actcacaaat taactttcat tactttccaa
    14101 ggtatcgtta ttacaattaa atatctttaa atatcaacaa gttaaagcat aaaaatcagc
    14161 ataaaccctg atttttatca aaaaaaaatg acggggataa ccatatttta tggcgataac
    14221 atcattcgtt atgtggttaa tacaaaaaag gggctgagac catatttcaa gcaattaccg
    14281 gataattacc aggcaaaatt tgagttaaat ttaatgagca agttctcaaa ttttattata
    14341 aataaaccat tttcagcgat aaatactgcg gcacgtcaca ttttttcacg ctatttgttg
    14401 gagaacaaac atttatttta tcaatatttt aaaatttcga atacaggtat tgatcatctc
    14461 gaacaattga ttaacgtcaa ctttttctct tctgacagga cgtcattttg tgaatgcaat
    14521 cgttttccat aaattcttct cccctcatag gcgacgaata gcattttgtg ttgaggatca
    14581 caaaacgaat aattgctggt cgccgcgata aggtcagacg aagtcaacaa gggggatttt
    14641 cactgtgctt tacatcagcg taggacacag aatgttgctt cgtttactgt aacgccgggt
    14701 aaatgagcgt tttttgatag tgcgtaagtg tcgtgcgagg aaaataagca tctattattg
    14761 tttgtcatga cgacaaggca ggcatcaatg aaaggcttcc caattgcaca tatttttcac
    14821 ccttcaatcc cgccaatgca cgcagtggtt aacaatcaca atagaaatat tgattattgg
    14881 acggtaaaaa gaaagtttgc agaaattgtc tccaccaatg acgttaataa aatttacagt
    14941 ataagtaatg aactccggag agtattatct gcaattactg cattgaattt ctatcaaggc
    15001 gatgttcctt ctgtcatgat ccgaatccaa ccggaaaata tgagtccatt catcatagat
    15061 atttctacag gagaacatga tgattatatc atacaaacat tagatgtagg cacttttgca
    15121 ccttttggtg aacaatgtac ttgctcagcc gtcaataaaa aagagctgga atgtattaag
    15181 gagacgattt ctaagtattg cgcaaagttc acccaaaaag aagccatctt aacaccccct
    15241 aggcacttta ataaaacctg cattacttca gattgttggc aaattctctt tttttcaccc
    15301 gatcatttta ataatgattt ttattgatta tttataaagg ataaaataat gttccctgtt
    15361 tcatcaattg gtaatgatat tagtagtgat ttagtccgtc gaaaaatgaa tgaccttcct
    15421 gaaagcccca taggaaataa cctcgaagca ctagcaccag gtatagaaaa actaaaacag
    15481 acctctattc aaatggtcac tttacttaac gcgttacaac caagtggaaa atgcattatc
    15541 actggtgatt ttcaaaaaga attagcgtac ttacaaaatg taattcttta taatgactcg
    15601 tctcttcgtc tggatttttt aggttataac acccaaatta ttcaacgatc ggacaatacc
    15661 tgcgaactta ccattaatga accgttaaaa aactaggaaa tatctacagg caatatcaat
    15721 gttaattgcc cattaaaaga tatttacaat gaaatcagga ggttgaacgt aatctttagt
    15781 tgtgggactg gagatatagt tgatctatcc tctctggact tacgtaatgt cgatttagat
    15841 tattatgatt tcacagataa acatatggct aatactattc taaatcattt taaattggat
    15901 tcaacaaatt ttactaatgc caacatgttt caggttaatt ttgttagttc aacacaaaac
    15961 gccacaatct cctgggatta tttactaaaa ataacgcctg ttttaataag cattagcgat
    16021 atgtattctg aagaaaaaat caagtttgtc gaaagttgtt taaatgagct tggagacatt
    16081 accgaagaac aattaaaaat tatgagattt gcaattataa aatctatacc aagggcaact
    16141 cttacagata aattagaaaa tgaattaaca aaagaaatat ataaaagctc atcgaaaatc
    16201 aacaattgct tgaacagaat taaattacca gagatgaaag aattctcatc aaaaaaaata
    16261 tatgattaca tcgatacaat cattgaagat tatgaaaata tcaaagaaaa tgcttatctg
    16321 gtcgtccccc aaattaatta tactatggat ttaaacatag aagactctag ctcagaagag
    16381 ttaataccct cgagaaagac gaaaattctc cggacaatgg ctttgaggtc ggggaatata
    16441 acacatatga agcatataac tcagagaatc aatattttac cagagaggac tatacgtata
    16501 attacgacct tttaaatgca atatagactt aataacttct tacaaagtct attattaata
    16561 ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa cttatcgacg cgggtttgaa
    16621 actgatggca accgcaaaaa tgcctgatgc gctacgctta tcaggcctac gccatctctg
    16681 caatatattg aatttgcgtg cttttgtagg caggataagg cgttcacgca gcatccggca
    16741 tgaataaagc gcactcaaca atctgaaacc cgccggaacg gtttattacg tacatcaggt
    16801 gaaactgacc gataagccgc tttcttttgg gtatagtgtc gtggacagtc attcatcttt
    16861 ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt acgtatatca ggtgaaactg
    16921 accgataagc cgctttcttt tgggtatagc gtcgtggaca gtcattcatc tttctgcccc
    16981 tccaaaagca aaaacccgcc gaagcgggtt tttacgtaaa acaggtgaaa ctgaccgata
    17041 agccgggttc tgtcgtggac agtcattcat ctaggccagc aatcgctcac tggctcaagc
    17101 agcctacccg ggttcagtac gggccgtacc ttatgaaccc ctatttggcc ttgctccggg
    17161 tggagtttac cgtgccacgg actgttacca gccgcgcggt gcgctcttac cgcacccttt
    17221 cacccttacc tgatcccgct tgcacgggcc atcggcggtt tgctctctgt tgcactggtc
    17281 gtgggtttcc cccccaggcg ttacctggca ccctgcccta tggagcccgg actttcctcc
    17341 cctccgcccg tctcccccga agaggacgac gacgaagcgg cgactgtctg gtcagcttcg
    17401 gcgcgcagta tagagggttt gcgcgccctt gtcaccccgt gttgcgcatt ccaatcgcca
    17461 gtgtcgcggc aatattacgt gaagcacggc agatattgtc ataagccccg cggaatgctt
    17521 cgtccaacgt acctatgctg gtcaatacgc tgaagaccgc atcaatgcca tgctgatgta
    17581 caacgccaac atcatcggtc aggctacccg caatgccaat caccggttta tggtacttct
    17641 tcgccacgtt tgcgacgcca atcggtacct tgccgtgaat actctggctg tcaatacgcc
    17701 cttcaccggt gatcaccagc gtacaatcgt gaatatgttc ctccagattc agcgccgtag
    17761 tgacgatttc aataccactt ttcagttccg caccaagaaa cgccatcagc accgcaccca
    17821 taccgcccgc agctcctgcg ccggggacat cttttacatc aacatgcagc gcttttttaa
    17881 tgacatcggc atagtgagag aggttattgt ccagctcaac aatcatcgct tcactggctc
    17941 ccttttgtgg gccaaagatg cgcgatgcgc cgttatcgcc caccagcgga ttggtgacat
    18001 cacaagcgac gcgaatgacg caatctttta agcgcggatc gaggccggaa atatcaatat
    18061 cattcagagt attaagacta ccgccgccaa aaccaatttc attgccgttg gcgtcgcata
    18121 atttcgcccc cagcgcctgt accatgcctg cgccgccatc atttgtagcg ctaccgccaa
    18181 tgccgataat aatgtttgtc gcgccgctct ccagcgccta caggattaac tcgcctgtgc
    18241 cgcgtgaagt ggtcacgagt ggatcgcgtt tttccgcagg taccagctcc agcccactgg
    18301 ccgccgccat ttcaataaac gcggttttgc catcgccgga gatcccccaa ctggcattca
    18361 ctttctcgcc cagcggccct gtaacccagg cgtgacgttc agaaccctgg gtggctgcaa
    18421 tcatcgcttc caccgttcct tcgccaccgt cggcaaccgg aatagaaacg tactgtgcat
    18481 caggaaaaat ttcccgaaat cctttttcta tcgcctgcgc aacctcgctg gcagataaac
    18541 tttctttata agagtctggg gcgattacga ttttcatacc tatgcctgtt accacatgac
    18601 gccggagggc gtttctctta ttcggcctgg attccaggcc cggattgcaa tacgccatcc
    18661 gggcacgacg tcattaacga gtaacttcga ctttcgccag tttttcgtag tagcacgcca
    18721 gggcgctatg atccgccgtt cctaaaccat ctgctcgcag tgcctgcatc atctccataa
    18781 ccgcagctgt gagcggcagt tgcgcgccga cgccgtggga agtatccagc gcatttgcca
    18841 gatccttaat atgcagatca atacggaagc ccggcttgaa gttgcggtcc atcaccatcg
    18901 gcgctttggc atccagcacg gtactgcccg ccagtccacc gcgaattgcc tgatacacca
    18961 ggtccgggtt aacgcccgct ttagttgcca gcgttaacgc ttctgacatc gcggcaatat
    19021 tcagcgccac aatgacctga ttcgccagtt tggtgacgtt acctgcacca attgccccgg
    19081 tatgcaccac ggaacccgcc attgctttca tcaaatcata gtatttgtcg aaaatagcct
    19141 tgtcgccgcc caccatcact gacagcgtac cgtcgatggc tttcggttca ccgccgctca
    19201 ccggagcatc cagcatatca atgcctttcg ctttcagcgc ttcgctgatt tcacggcttg
    19261 ccagcggtgc gatagaactc atatcgatca ataccgtacc tggcttcgcg ccttcaataa
    19321 tgccattctc acccagcgcc acctctttca catgagggga gtttggcagc atggttatga
    19381 tgacgtcgca ctgttcagcg atcgctttag ccgtagaggc tgtttctgca cctgcagcaa
    19441 tcacgtcagc aatagcttct gggttacggt cagcaaccac cagcgagtaa cctgctttca
    19501 gaaggttttt actcattggt ttacccataa tccccaggcc aataaaacca actttcatag
    19561 tcatatcaat catctctctt gttgcggtgg tggttatttt ttaaaggtat cagccagttt
    19621 atgagtggca gagcggaaga cgccgagatc gctgccgaca gccacaaacg tcgcgcccca
    19681 ttccagataa cgacgcgcat cggcttcgac cggcgcgagg ataccgctgg gtttgccgtg
    19741 cgcgctggca cggttaaaaa tgtgctgaat tgctttttgt acatccgggt gtgatgcatt
    19801 gccgagatgg cctaatgccg cggccagatc gctggggccg acgaagatgc cgtctacgcc
    19861 ttcggtagcg gcaatggcat cgacgttatc tacgccctgc tgactttcta tctggaccag
    19921 aatagtgatg ttcttgttcg actgagcgaa ataatccgcc acggtgccaa acatattggc
    19981 gcggtgagaa acggagacgc cgcgaatgcc ttccggtggg taacgggttg atgccaccgc
    20041 ctgctctgct tcctcttttg tttctacaaa aggaatcagg aagttataga aaccgatatc
    20101 cagaagacgc ttaataattt ccggctcgtt ggtcggcact cacactactg gcgcgctggc
    20161 gctgcctttc aaggccatta actgcggaat aaacgtggag atatcgtttg gcgcatgttc
    20221 gccatccagc accagccagt caaacccagc caaaccaaga acttcagtgc taatcgggtt
    20281 agagagtgct gaccagcaac caatttgtac ctgtttcgca gccagtgcgg ctttgaattt
    20341 attcgggaaa acatcgttat tcatcgctta tacctttgct tatttctgca attccatacg
    20401 tttaatgtcg ccaactacga agaggtagca gaccatcgcc atcagcgctg aacatcccac
    20461 gaaaaccagt gctgcattga aggagtgcag ttcacttacc aggtagccaa tcaccagtgg
    20521 agtgacaatg gaggcaacat tgccaaagac gttaaagacg ccgccacaga ggccaacaat
    20581 ctctttcggc gcggtgtcag aaatcaccgg ccagcccaga gcaccaaatc ctttgccaaa
    20641 aaaagccagc gccatcagca tgaccaccag cgtggtgttg ttggtgtagt tacataagat
    20701 gatggtggaa gccagcaaca ttcccagcac aatcggtagc ttacgtgcca gggtcaggga
    20761 taaaccgcgt ttgatcagat aatccgagaa gacacctccc agcacgccgc ccgcaaaacc
    20821 acacagtgcc ggaatcgagg cgaccagacc cactttcaga atcgacatgc ctttttcctg
    20881 caccagataa atcgggaacc aggtgaggaa gaaccaggtg atggtgttga taaaatattg
    20941 tccgaaaaat acgcccaaca tcatgcggtt agagagcaat tgcttgatgt aatgcagttt
    21001 gagaccgctt gctgccgcgc tgcccggctt tttgtggtcc atatcgacca ccgcgccatt
    21061 ttcagagata aacttcagct cttcctcaga catacgtggg tgatctgtcg ggttatgaat
    21121 caacttgatc cacagcgccg tcagcacaaa acaaatcacc cccataacgg taaagacgtg
    21181 ctcccatccc caggcgaaag tcagccagcc aagcagaggc gaaaagagcg ccagcgagaa
    21241 atattgtgcc gagttaaaga tggcggaggc agtaccacgt tctttcgtcg ggaaccaggc
    21301 ggcgacaata cgggcgttcg ccgggaatga tggagcttcc gagaatccca gcataaagcg
    21361 cataaagaac atggagatcc ctgcccaggc cagcgggaac atatcaacaa agccttgcag
    21421 gaaggtgaac agcgaccaga aaaagaggct gtaggtgtaa acttttttcg agccaaactt
    21481 atcaagcaac cagccgccgg ggatttgcat cagcaagtag gcccagccaa aagcggagaa
    21541 gatgtaaccc atcgaaaccg cacttaactg caactctttt gccacttcgg taccagcaat
    21601 agacagcgtt gcacgatcgg cgtagttaac ggcggtaaca ataaaaataa tcagtaatat
    21661 taaatagcgg gtatgcacac ctttcctttt ttcgtcaacg gtgtccagaa tcattttatt
    21721 tacctcgggt acttatgctg atttttatta ttatggggaa ggtgttattt ataagtttca
    21781 tttatgccgt aacgacaatg aactcgggaa ttagtataag cagcgcgaga ataataatca
    21841 ttgtgcaaat gctaatttaa ttaatacttt ttaaatatta ttttgagcat atgcacataa
    21901 ggttgcgcgc taaagcacag atttgcgctt taccttgccg ggcggcactg caatccctga
    21961 aatgattgac attgatcaca tttctgcgtt taaactcctg acattcttat ttcacccaat
    22021 gaaatcattt atttttaaat gagaccaggt cctcatttta ataacccctg gctggagaat
    22081 attgcacaat ggccaacatc gaaatcagac aagaaacgcc aactgcgttt tatataaaag
    22141 ttcacgacac agataatgtg gcaattattg ttaatgataa tggcctgaaa gcaggaacgc
    22201 gttttccgga tgggctggaa ttaattgaac atattcccca ggggcataaa gtcgcattgc
    22261 tggacattcc ggctaatggt gaaattattc gttatggcga agtgattggt tacgccgtgc
    22321 gtgcaatccc acgcggaagc tggatcgacg aatcaatggt tgtactaccg gaagcgccgc
    22381 cgttacacac gctgccactg gcaaccaaag tcccggaacc cttaccgccg ctggaaggat
    22441 acacctttga gggctatcgc aatgccgatg gcagcgtggg caccaaaaac ctgctcggca
    22501 tcaccaccag cgtccactgt gtggcaggcg tggtggacta tgtagtaaaa atcattgaac
    22561 gcgatctgct accgaaatac ccgaacgtcg atggcgtggt ggggctgaat catttgtacg
    22621 gttgtggcgt ggcgattaac gcaccggcag cagttgtacc tattcgtacc attcacaata
    22681 tttcgctgaa ccctaacttt ggcggcgaag taatggtgat tggcctgggt tgtgaaaagt
    22741 tgcagcctga gcgcctgctg actggaacgg atgatgtgca agctattcca gtagaaatcg
    22801 ccagcattgt cagtttgcag gatgaaaagc atgtcggttt tcagtccatg gtcgaggata
    22861 ttttgcaggt cgccgaacgc catctacaaa aactgaatca acgtcagcga gaaacctgtc
    22921 cggcttcaga actggttgtc ggcatgcatt gcggtggcag cgatgcgttt tctggcgtaa
    22981 cggcaaaccc ggcagttggc tatgcgtctg atctactggt gcgctgcggc gcaacggtga
    23041 tgttttcaga agtcacggaa gtgcgtgacg cgatccatct gctgacacca cgcgcagtga
    23101 acgaagaggt cggcaaacgg ctgctggagg agatggagtg gtacgataac tatctcaata
    23161 tgggaaaaac cgaccgcagc gccaaccctt cgccgggcaa caagaaaggc ggtctggcaa
    23221 acgtggtaga gaaagcgctc ggctccattg ctaaatcggg caaaagtgca attgttgaag
    23281 tgctgtcgcc cggtcaacgc ccgactaaac gcggattaat ttacgccgcg acgccagcca
    23341 gcgattttgt ctgtggcacg caacaggtgg cttcgggtat caccgtgcaa gtgtttacga
    23401 ccggtcgtgg tacgccgtac ggcctgatgg cggtacccgt cattaaaatg gcaacccgca
    23461 ccgagctggc gaaccgctgg tttgatttaa tggatattaa tgcgggcacc atcgctaccg
    23521 gcgaagaaac tattgaagag gtgggctgga agttgttcca ctttattctc gacgtcgcca
    23581 gcgggaagaa gaaaaccttc tcggatcaat ggggactgca taaccagctg gcggtgttta
    23641 acccagcacc ggtgacctga taattcaatt cgtattatgc ccgatagcac acgtttatcg
    23701 ggcatattga gtcacagcga agacgcgtga tccccgacca gaatcacttc aaccccagcc
    23761 tttcgcagtc cttccagact atccgcagga atgccttcat caacaatgat catgtcgata
    23821 cgttgagtat caatgatctt atgtaaactg gaacgattga atttactgga atcggtgacc
    23881 acgatgatcc gttccgcaac ttcgcacatc cgacggttta aacgggcttc atcttcatta
    23941 tgtgtgctga cgccgcgctc cagatcgatc gcatctacac caagaaacag catatcgaag
    24001 tggtaatttt gcagcgattg ctcagcctga tcgccgtaaa aagattgcga ctgacggcgc
    24061 aaatgcccgc cggtcatcag cagctcaacg ccttccgctt ccagcaacgc attagccacg
    24121 ttcataccgt tggtcatcgc aattacgtca gtgtacttgc gcatcagacg agcaatctca
    24181 aaagtggtgg tcccggaatc gaggatcacc cgatgacctg gctgaatcaa ctcaacggca
    24241 gctttcgcaa cgctgcgttt catcgcggtg ttcagtgcgc ttttatcttc cactgatggc
    24301 tcgactgacg gcgtcgtgct atcgcagatc aacgcgccac cataggcacg cacagcgatc
    24361 ccctgctttt ccagaaacgc cagatcattg cggatcgtca cagtagatac gccatacaat
    24421 gccgacagat cgttaacctg cacactccct tgctgtcgca gacgctgaat gatctgttct
    24481 cgtcgctcgc tggtgcctgt cactcgcttc tcacctgaag cgtcggtatt actcatagta
    24541 agtcctttcg taaaactttc gtttcatttc gttttgccta ttaacgcctt tctattaagc
    24601 aaatgcaagc ccaccttgcc cattggcgca agctactctc gtttcagtga ctttcattac
    24661 gtttcttttg tgaatcagat cagaaaacta ttatctttcg ttttattttt atctcaccat
    24721 gacgcagtat caattgaaac aaaacgaaag atcaatatcg cagtaatctg aactggagag
    24781 gaaagtgaaa catctgacag aaatggtgag acagcacaaa gcgggcaaaa caaatgcaat
    24841 ttatgccgtt tgttccgcac atccgctggt gctggaggct gcaatccgct acgccagtgc
    24901 aaaccaaacg ccgttactga ttgaagcaac ctccaatcag gtagaccagt tcggcggtta
    24961 taccggaatg acgcccgccg attttcgcgg ctttgtttgt cagctcgccg actcgttgaa
    25021 tttcccgcag gatgcgctga ttctgggtgg tgaccatctg gggccaaacc gctggcaaaa
    25081 cctgccagcc gctcaggcaa tggccaatgc cgatgatttg attaaaagct acgttgcggc
    25141 aggattcaaa aaaatccacc ttgattgcag catgtcctgt caggacgatc caattccctt
    25201 aactgatgac atcgtggctg aacgcgccgc ccgtctggcg aaagtggcgg aagaaacctg
    25261 tcgcgaacac tttggcgaag ccgatctgga gtatgtcatt ggtaccgaag tgccggtacc
    25321 tggcggcgcg catgaaacct taagcgagct ggcggtcacc acgccggatg ccgcccgcgc
    25381 cacgctggaa gcccatcgtc acgcctttga aaagcaaggt ttgaatgcca tctggccacg
    25441 cattattgcc ctggtggttc aacccggcgt cgaattcgat cacaccaacg ttattgatta
    25501 tcagcccgcc aaagcgagcg ccttaagcca gatggtcgaa aactacgaaa cgctgatttt
    25561 cgaagcgcac tctaccgatt atcaaacgcc gcaatctctg cgccagctgg tgattgacca
    25621 ctttgccatt ctgaaagttg gcccggcgct gaccttcgcc ctgcgtgaag cccttttttc
    25681 tctggcggcg attgaagaag aactggtacc agcgaaagcc tgttccggtc tgcgtcaggt
    25741 gctggaaaac gtgatgctcg accgcccgga atactggcaa agccactacc acggtgacgg
    25801 caacgcgcgt cgtctggcgc gtggttacag ctactcagat cgcgtgcgct attactggcc
    25861 ggacagccag attgatgacg ctttcgctca tctggttcgt aatctggcgg attcaccaat
    25921 tccgctaccg ctcatcagcc agtatctgcc gctgcagtac gtgaaagttc gctccggcga
    25981 gctgcaaccg acgccacggg aactcattat aaaccatatt caggacatcc tggcgcagta
    26041 ccacacagcc tgtgaaggcc aataagcaaa acaaagagga acatgctatg ccaaatattg
    26101 ttttaagccg gattgatgaa cgcttgattc acggtcaggt cggcgttcag tgggtcggat
    26161 ttgcgggggc aaatctggtg ctggtagcca acgatgaggt tgccgaagat ccggtacaac
    26221 aaaacctgat ggaaatggta ctggcagaag ggatcgccgt acgtttctgg acgctgcaaa
    26281 aagttatcga caacattcat cgcgccgccg atcgacagaa aatcctgctg gtttgtaaaa
    26341 cacccgccga tttcctgacg ctggtgaaag gtggcgttcc ggtgaatcgc attaacgttg
    26401 gcaatatgca ctacgccaat ggcaaacaac aaatcgccaa aacggtttct gtggatgcgg
    26461 gcgatatcgc agcatttaac gacctgaaag ccgctggggt ggaatgcttc gttcagggcg
    26521 tcccgacaga gcctgctgtg gacctcttta aattactttg agggattcat catggaaatc
    26581 agcctgttgc aggcatttgc gttgggcatt atcgccttta tcgctggcct agatatgttt
    26641 aacggcttaa cccatatgca ccgcccggtg gtcctcggcc cgttggtcgg gctggtactt
    26701 ggcgatctgc ataccggaat tttaaccggc gatacgctgg aactagtgtg gatggggctg
    26761 gccccgctgg cgggcgcaca gccgcctaac gtgattatcg gtactatcgt cggcacggcg
    26821 tttgccatta ctactggcgt gaaacccgat gtcgcggtcg gtgtcgccgt acctttcgct
    26881 gtcgcagtac agatggggat taccttcctg ttctcggtga tgtctggcgt gatgtctcgc
    26941 tgcgaccgga tggcggagaa cgccgatact cgcggcattg aacgcgtgaa ctatctggca
    27001 ctgctggcac tcggcacttt ctattttctc tgcgctttcc tgcctatcta cttcggcgcg
    27061 gaacatgcaa aaaccatcat tgatgtcctg ccgcaacgat taatcgacgg cctcggcgtc
    27121 gcgggcggca tcatgccagc aatcggtttt gccgtgctgc tgaaaatcat gatgaaaaac
    27181 gtctacatac cctacttcat cctgggcttt gttgccgcaa cctggctcaa gttaccggtg
    27241 ctggctattg ctgccgccgc gctggcgatg gcgctgatcg atctgctgcg taaatctcct
    27301 gaaccgactc aacctgcggc acagaaagag gaatttgaag atggcatcta atcaaaccac
    27361 cctgccgaac gtcagcgaaa atgaagaaac actgctgact ggcgtcaatg aaaacgtgta
    27421 tgaagatcgg agcattgccg cggagctgac gaaaaaagat atcaaccgcg tcgcctggcg
    27481 ttccatgctg ttacaagcgt cttttaacta cgaacgtatg caggcttctg gctggttgta
    27541 cggtctgctg cctgctctga aaaagatcca cactaataaa cgcgacctgg cgcgcgccat
    27601 gaaggggcat atgggtttct tcaataccca tccgtttctg gtgacgtttg ttatcggcat
    27661 tatccttgcg atggagcgtt ctaagcagga cgttaacagt attcagagca ccaaaattgc
    27721 cgtcggtgcg ccgcttggcg ggattggcga tgcgatgttc tggctaacgc tactgccgat
    27781 ttgtggcggg ataggcgcaa gcctcgcgct acaaggctct attcttggcg cagtcgtctt
    27841 tattgtgctg ttcaacgtgg tacacttggg gctgcgtttt ggtctggcgc attatgctta
    27901 ccgcatgggc gtggcggcga ttccccttat taaagcgaat accaaaaaag tcggccatgc
    27961 tgcgtcaatc gttggaatga cggtaatcgg cgcactggtg gcaacctatg ttcgtttaag
    28021 caccacgctg gaaatcacgg cgggcgacgc agtagttaag ttacaggctg atgttatcga
    28081 caaactgatg cctgccttct taccgctggt ctacaccctg accatgttct ggctggtacg
    28141 ccgcggctag agtccgctgc gcctgattgc ggttaccgtg gttctaggga tcgtcggtaa
    28201 attctgccat ttcctttaaa tacaatgagg tattagatgt taagtattat tttgacaggg
    28261 catggcgggt ttgccagtgg catggaaaaa gcaatgaagc aaatcctcgg cgatcaatcg
    28321 cagtttattg ccatcgattt tcccgaaacc tcaagcgccg cgctgcttac atcgcagctt
    28381 gaagaggcca tcgcccaact ggattgtgaa gacggcattg tttttctgac ggatttgctg
    28441 ggcggcacgc cgtttcgcgt agcttcaacg ctggcgatgc aaaaaccggg ctgtgaagtg
    28501 attaccggca ccaatttgca actgctactg gagatggtgc tggagcgcga agggttaagc
    28561 ggtgaagaat tccgtgtaca ggcgctggag tgtggacacc gagggctgac cagtctggtg
    28621 gacgagttag gccgctgcca tgaagaatgt ccggtcgagg aaggaatatg acacacgttc
    28681 tgcgcgccag aaggctgctg actgaagagg gatggttcga tgaccatcag ttgcgtgttg
    28741 ctgacggtgt catcgcagca atcgaaccga ttccagtggg cgtgactgaa cgcgatgcgg
    28801 aactgctctg ccccgcttac atcgacaccc atgtacacgg tggtgcgggc gttgatgtaa
    28861 tggatgacgc gccggatgta ctcgacaagc tggcaatgca caaggcacgc gaaggtgtcg
    28921 gcagttggtt accgaccacc gtaaccgcgc cgcttaatac cattcatgcg gcgctgaaac
    28981 gtattgctca acgttgccaa cgcggcggac ctggtgcgca agtgctgggg agttatctcg
    29041 aaggaccgta cttcacgccg cagaataaag gcgcgcatcc
//
LOCUS       DAJVRP010000049        28982 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVRP010000049 DAJVRP010000000
VERSION     DAJVRP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.049"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..218
                     /locus_tag="OQN12_002170"
     CDS             <1..218
                     /locus_tag="OQN12_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1654480.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="OQN12_002171"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="OQN12_002171"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1654481.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="OQN12_002172"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="OQN12_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1654482.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="OQN12_002173"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="OQN12_002173"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1654483.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="OQN12_002174"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="OQN12_002174"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1654484.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="OQN12_002175"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="OQN12_002175"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1654485.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="OQN12_002176"
     CDS             7229..8179
                     /locus_tag="OQN12_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1654486.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="OQN12_002177"
     CDS             complement(8220..8690)
                     /locus_tag="OQN12_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1654487.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="OQN12_002178"
     CDS             complement(8745..9947)
                     /locus_tag="OQN12_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCS1654488.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="OQN12_002179"
     CDS             complement(10034..10576)
                     /locus_tag="OQN12_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654489.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="OQN12_002180"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="OQN12_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1654490.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="OQN12_002181"
     CDS             complement(11319..11921)
                     /locus_tag="OQN12_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS1654491.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="OQN12_002182"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="OQN12_002182"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1654492.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="OQN12_002183"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="OQN12_002183"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1654493.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="OQN12_002184"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="OQN12_002184"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS1654494.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="OQN12_002185"
     CDS             16319..17209
                     /locus_tag="OQN12_002185"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1654495.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="OQN12_002186"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="OQN12_002186"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1654496.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="OQN12_002187"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="OQN12_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1654497.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="OQN12_002188"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="OQN12_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1654498.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="OQN12_002189"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="OQN12_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1654499.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="OQN12_002190"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="OQN12_002190"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1654500.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="OQN12_002191"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="OQN12_002191"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1654501.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="OQN12_002192"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="OQN12_002192"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1654502.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="OQN12_002193"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="OQN12_002193"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1654503.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="OQN12_002194"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="OQN12_002194"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1654504.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="OQN12_002195"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="OQN12_002195"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1654505.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="OQN12_002196"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="OQN12_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1654506.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="OQN12_002197"
     CDS             complement(27384..27845)
                     /locus_tag="OQN12_002197"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1654507.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="OQN12_002198"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="OQN12_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCS1654508.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         6999 a         7814 c         7249 g         6920 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 ga
//
LOCUS       DAJVRP010000050        28434 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVRP010000050 DAJVRP010000000
VERSION     DAJVRP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.050"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            564..1334
                     /locus_tag="OQN12_002199"
     CDS             564..1334
                     /locus_tag="OQN12_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS1654509.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OQN12_002200"
     CDS             1325..1798
                     /locus_tag="OQN12_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1654510.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OQN12_002201"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OQN12_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1654511.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OQN12_002202"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OQN12_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1654512.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OQN12_002203"
     CDS             3233..3727
                     /locus_tag="OQN12_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1654513.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="OQN12_002204"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="OQN12_002204"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1654514.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OQN12_002205"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OQN12_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="OQN12_002206"
     CDS             7571..8485
                     /locus_tag="OQN12_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1654515.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="OQN12_002207"
     CDS             8700..10061
                     /locus_tag="OQN12_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1654516.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="OQN12_002208"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="OQN12_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCS1654517.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="OQN12_002209"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="OQN12_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1654518.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="OQN12_002210"
     CDS             12611..13900
                     /locus_tag="OQN12_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1654519.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="OQN12_002211"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="OQN12_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1654520.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16216
                     /gene="hpaD"
                     /locus_tag="OQN12_002212"
     CDS             15365..16216
                     /gene="hpaD"
                     /locus_tag="OQN12_002212"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1654521.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16226..16606
                     /locus_tag="OQN12_002213"
     CDS             16226..16606
                     /locus_tag="OQN12_002213"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1654522.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16674..17477
                     /gene="hpaH"
                     /locus_tag="OQN12_002214"
     CDS             16674..17477
                     /gene="hpaH"
                     /locus_tag="OQN12_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1654523.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17488..18291
                     /gene="hpaI"
                     /locus_tag="OQN12_002215"
     CDS             17488..18291
                     /gene="hpaI"
                     /locus_tag="OQN12_002215"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS1654524.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18313..19668
                     /gene="hpaX"
                     /locus_tag="OQN12_002216"
     CDS             18313..19668
                     /gene="hpaX"
                     /locus_tag="OQN12_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1654525.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19678..20568
                     /gene="hpaA"
                     /locus_tag="OQN12_002217"
     CDS             19678..20568
                     /gene="hpaA"
                     /locus_tag="OQN12_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCS1654526.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20595..21532
                     /locus_tag="OQN12_002218"
                     /pseudo
     CDS             20595..21532
                     /locus_tag="OQN12_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21759..23317
                     /gene="hpaB"
                     /locus_tag="OQN12_002219"
                     /pseudo
     CDS             21759..23317
                     /gene="hpaB"
                     /locus_tag="OQN12_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23335..23847
                     /locus_tag="OQN12_002220"
     CDS             23335..23847
                     /locus_tag="OQN12_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1654527.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24240..26390
                     /gene="btsT"
                     /locus_tag="OQN12_002221"
     CDS             24240..26390
                     /gene="btsT"
                     /locus_tag="OQN12_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1654528.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26440..26643
                     /locus_tag="OQN12_002222"
     CDS             26440..26643
                     /locus_tag="OQN12_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1654529.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26654..27610
                     /gene="yjiA"
                     /locus_tag="OQN12_002223"
     CDS             26654..27610
                     /gene="yjiA"
                     /locus_tag="OQN12_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1654530.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27679..>28434)
                     /locus_tag="OQN12_002224"
     CDS             complement(27679..>28434)
                     /locus_tag="OQN12_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCS1654531.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6961 a         7432 c         7127 g         6914 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggccccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg tctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcaa tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
    15841 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
    15901 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
    15961 ctacacccgc gagttcgacc gccagatgga cgagcgtgtg gtgaagctgt ggcgcgaagg
    16021 ccagttcaaa gagttttgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
    16081 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
    16141 ggtggagttt attaccgagc tgttcccaag ctctggcacc ggtcaggtta acgctgtttt
    16201 cccgctgccc gcgtaaggac gttttatgcc gcactttatc gtggaatgta ctgaaaatat
    16261 tcgtgaagaa gcccgcttac ctgagctgtt tgccagtgtt aacactgcac tggcggcaac
    16321 ggggattttt ccgcttgggg gaatacgtag ccgggcgcac tggatcgata cctggcaaat
    16381 ggctgatggc aagcacgatt acgcctttgt gcatatgacg ctaaaaatcg gcagcggacg
    16441 cagtctggaa agccgtcagg aagtgggcga aatgctgttt gatctgatta aaacgcactt
    16501 cgcctcactg atggagagcc gttacctagc gctttcattt gagattgcag agctgcaccc
    16561 gacgttgaat ttcaaacaga acaacgtaca cgcgttgttt aaataaattt gcaccagctt
    16621 ccaggccgga tgataaggcg ctgccgcatc cggcaatgcc acaggatatc gctatgttcg
    16681 ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac
    16741 agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgcc tacgccgtgc
    16801 agcgcgaatg ggttcgcctg aaaatcgccg aaagtcgcac gctgaaaggc cacaaaatcg
    16861 gtctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggtgcac
    16921 tgatggacga catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc
    16981 cgcgcattga agtggagctg gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca
    17041 cgctgttcga cgtttacaac gccacggact atgtgatccc ggcgctggag ctgatcgacg
    17101 ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagtattc gacaccattt
    17161 ccgataacgc cgccaacgcc ggagtgatcc tcggaggtcg tccgatcaaa ccagacgaac
    17221 tggatctgcg ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg
    17281 tcgccgctgg tgtgctgaat catccggcaa acggcgtggc ctggctggcg aacaaactcg
    17341 caccgtatga cgtacaactg gaagccgggc aaatcattct cggtggttcg ttcacccgcc
    17401 cggttccggc gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggctccatta
    17461 gctgccgctt tgtttaagga gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca
    17521 ggccgtccgc agattggatt atggctgggg ctgagcagca gctacagcgc ggagttactg
    17581 gccggaccag gattcgactg gttattgatc gacggtgagc acgcgccgaa taacgtgcaa
    17641 accgtgctca cccaactaca ggcgattgcg ccctacccca gccagccagt ggtacgccca
    17701 tcgtggaacg atccggtgca aatcaaacaa ctgctggacg taggcacaca aaccttactg
    17761 gtgccgatgg tacaaaacgc cgacgaagcc cgtaaagcgg tacgcgccac ccgttatccc
    17821 cccgccagta ttcgcggtgt gggcagtgcg ctggctcgcg cctcgcgctg gaatcgcatt
    17881 cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt
    17941 gaggcaatga agaacttacc gcagattctg gacgtggaag gggtcgacgg cgtgtttatc
    18001 ggcccggcgg atctgagcgc cgatatgggt tatgccggta atccgcagca cccggaagta
    18061 caggccgcca ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc gccggggatc
    18121 ctgatcgcca atgagcaact ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc
    18181 gtcggcgttg acaccaccct gctcgcccgc gccgccgaag cgctggcagc acggtttggt
    18241 aatagttctt ctatttcgat ggcgaagcaa aacaacaact ctgtctacta aacgtacgat
    18301 gtggagcgca tcatgacgac ctctaccctg caatataaag ataataaaac tgttgaaatt
    18361 gaacatcgtg tcattaataa attatttcgt cgcctaatca tttttctttt tattctgttt
    18421 gttttttcgt ttcttgatcg catcaatata ggatttgccg gactgacaat gggcaaggat
    18481 cttggcctga cgtccacaat gtttggcctc gccgcaacac tcttttatgt cacgtacgtc
    18541 atgtgcggga tccccagtaa tatgatgtta gcgaaaattg gcgctcgtcg atggatcgct
    18601 gggatcatgg tggtctgggg aattgcttcc acctgcacaa tgtttgcaac aagccccgaa
    18661 acactctatc tactgcgtat gctggttggt attgctgagg cgggtttttt acctggtatt
    18721 ttggtttatc tgacctggtg gttcccggca tatcatcggg ccagagccaa tgccttattt
    18781 atgatagcga tgccagtcac tatgatgctg ggattgatcc tttcaggcta cattctggcc
    18841 ttggatggac tgtggaatct aaaaggctgg caatggctct ttttgctgga aggtctgcca
    18901 tcggtcattc ttggtgtcgt cacctggttt tatctcaacg acacaccaga ccaggcgacc
    18961 tggcttgatg aaaacgaaaa acaggcttta aaaacgatga ttctccgtga acaagagagt
    19021 gcatttgccc atgcatctgt tccacgctca accttacgtg aagtcctgac cccagccatc
    19081 atgctatata cacttgctta tttttgcctg actaatactc tgagtgcgat taatatctgg
    19141 acgccgcaaa tcctacagag tttcaatact ggcagtagca acatcatgat tggtttgctg
    19201 gcggccatcc cccaattctg cacaattttt ggcatgatct ggtggagtcg tcgttccgac
    19261 cgtcttaaag aacgcaaaaa gcatacgatt cttccctacc tgtttgccgc tgccggatgg
    19321 ttactcgctt ctgcgacaga tcacagcctc attcaactga gcagcatcat tatggcctca
    19381 atgggatcat ttaccgcgat gactatcttt tggacaacac cggatcaagt tataagcttg
    19441 caatcgcgtg ccgttgcgat ggctgtcatc aatgctgtcg gcaatgtagg ttcagcagtc
    19501 agtccattac ttatcggtat tttgaaagat gcaaccggca gttttagctc aggtttgtgg
    19561 tttgtagcag gattgctggt actgggcgcg ttagtcatga cccgaatccc tatggggagt
    19621 cgggaacact ctctttcatc tgaacttatc gcaccaaaac cgcaataagg agtcatgatg
    19681 tccagtgaag ctatctttga acatggttat gaaaatattg atttggtccg ggaatatgat
    19741 gcccgctacg ctggtgaaga tgtgcattat gaaacatttg cccggttagc gaatttcttt
    19801 ggacgcgata tgcgccctca ctggcacgat agatattttc aactccatta tctggtcact
    19861 ggacgcatta ctttacaact ggatgagcac ttctattctg tgcgcgcacc gctttttgta
    19921 ctaacaccac catcggtgcc acatactttc tttactgatc ctgatagcga tgggcatgtt
    19981 ctgaccgtac gtcaggagtt aatttggccc ctggtagaaa agctctggcc tggtagtgga
    20041 gatgccgcaa tgctacaagg tatctgtctg tcactggagg gaatggaaca tacacaggcg
    20101 gcattaaatc attattggcc gataatatcc ctggagtttc aacaaaataa gcaaggacgg
    20161 gaaattctat tgcaatcact ggcgcaaagt atttttacaa tcctactgcg tgaagcacca
    20221 ccaaatgata tttcaacatg cagcgtgcgc ggcgagatgt gtttattcca gaagtttaat
    20281 cggctgattg acgaaaaata tcgccaacat ttaatggtcc ctgaatatgc aacaatgctc
    20341 ggtatgagtg aatcacggct taccgagctg tgccgacgtt ttgccaatca atcgccgaag
    20401 agattgattt tcgaacgagt gagccgggaa gccagacggc ttctgctcta tagcgagcaa
    20461 agcatcaacc agatagccct tgatcttggc tacaaagatc ccgcctattt cgcccgtttt
    20521 ttcaaccgaa tgatgggttg cccaccaaca caattccgtg gtcgctgacc atttatcaac
    20581 ctgagagact cgacatgaaa atatctttac gctcactatt tgtggcgtgc ctgattgcgc
    20641 cagtggcatt cacctacgcc tccgaaccgc aataaatgaa aattgtacta cttggaacga
    20701 aaggtggccc ttctttatta aaagctgacc gtttaccgat ggcaacggca cttattattg
    20761 gcaataaaac ctagttaatt gatgctggtt acggggccag cttacaactg gtaaaaaata
    20821 atattccact gcgccagatt gataatattt tactcaccca tttacacagt gatcatattc
    20881 tggactatcc atcagtctta atgaatgcat gggctagcgg tctgcaggac aaaactattc
    20941 atgtattcgg tccgccaggc acccgagcca tgaccgctgc cagttggcag gtcttcgatc
    21001 gggacatcac actgcgtatg gaagaagaag gaaaacccga tcctcgcaaa ctcgttacag
    21061 ctcaagatgt tggtgaaggc gtagtttata aagatgatac cgtgaccgtg actgcattaa
    21121 aggtccctca ttctcctttc cctgacggtg aggcttttgc ttatcgcttc gacacacaag
    21181 gcaagcgaat tgtcttctca ggagatacct cgtactttcc cccactggca actttctcag
    21241 aaggcgctga tatacttgtg catgaggccg tacatgagcc ctccgtcgct aagctggcag
    21301 acagtattgg caatggtaaa acactggcaa aagccattgc ttctcaccat acgactattg
    21361 aggatgtagg caaaattgcc aatgaagcaa aagtgaaaaa actcatcctt agccacctgg
    21421 taccaatcac cgttccggat gaaacatgga agcaagaagc acagaaaact attcaggccc
    21481 tatcgtcgtt ggtcatgaaa atatgacgat taccattcca ttcgccccat aaaaaattcc
    21541 gtccccgtta gcccttgtta gcggggaata ttatgatttg ccttactgta agttgatcac
    21601 gatcgcagtt tactatttaa ctccaccttg ctgtcgaaaa gtaccaacgt tcgcctgaaa
    21661 cgtctcttcc cgacaaccgc gttcaacgct tcaattaaat aacaaaaaca acacacaaaa
    21721 ttaacaatcg tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg
    21781 ccagtaccca acgtcctttc accggggaag agtatctgaa aagcctgcag gatggtcgcg
    21841 agatctatat ctatggcgag cgagtgaaag acgtcaccac tcatccggca tttcgtaatg
    21901 cggcagcgtc tgttgcccaa ctgtacgacg cgctgcacaa accggagatg caggactctc
    21961 tgtgttggaa caccgacacc ggcagcggcg gctataccca taaattcttc cgcgtggcga
    22021 aaagtgccga cgacctgcgc cagcaacgcg acgccatcgc tgaatggtca cgcctgagct
    22081 atggctggat gggccgtacc ccagactaca aagccgcttt cggttgcgca ctgggcgcga
    22141 atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg
    22201 aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgtcatt tgccgaccga
    22261 taaagtgaaa gacgtttaca tcaagctgga aaaagagact gacgccggga ttatcgtcag
    22321 cggtgcgaaa gtggttgcca ccaactcggc gctgactcac tacaacatga ttggcttcgg
    22381 ctcggcacaa gtgatgggcg aaaacccgga cttcgcactg atgttcgttg cgccaatgga
    22441 tgccgatggc gtgaaattaa tctcccgcgc ctcttatgag atggtcgcgg gtgctaccgg
    22501 ctcgccatac gactacccgc tctccagccg cttcgatgag aacgatgcga ttctggtgat
    22561 ggataacgta ctgatcccat gggaaaacgt gctgatctac cgcgattttg atcgctgccg
    22621 tcgctggacg atggaaggcg gtttcgcccg tatgtatccg ctgcaagcct gtgtgcgcct
    22681 ggcagtgaaa ctcgacttca ttacggcact gctgaaaaaa tcactcgaat gtaccggcac
    22741 cctggagttc cgtggtgtgc aggccgatct cggtgaagtg gtggcgtggc gcaacacctt
    22801 ctgggcattg agtgactcga tgtgttcaga agcgacgccg tgggtcaacg gggcttattt
    22861 accggatcat gccgcactgc aaacctatcg cgtactggca ccaatggcct acgcgaagat
    22921 caaaaacatt atagaacgca acgttaccag tggcctgatc tacctccctt ccagtgcccg
    22981 tgacctgaac aatccgcaga tcgaccagta tctggcgaag tatgtgcgcg gttcgaacgg
    23041 tatggatcac gtccagcgca tcaagatcct caaactgatg tgggacgcca ttggcagcga
    23101 atttggtggt cgtcacgagc tgtatgaaat caactactcc ggtagccagg atgagattcg
    23161 cctgcaatgt ctgcgccagg cacaaagctc cggcaatatg gacaagatga tggcgatggt
    23221 tgatcgctgc ctgtcggaat acgaccagaa cggctggact gtgccgcacc tgcacaacaa
    23281 cgacgatatc aacatgctgg ataagctgct gaaataacgc agcaggaggt taagatgcaa
    23341 ttagatgaac aacgcctgcg ctttcatgac gcgatggcca gcctgtcggc agcggtaaat
    23401 attatcacca ccgagggcga cgccggacaa tgcgggatta cggcaacggc tgtctgctcg
    23461 gtcacggata caccaccgtc gctgatggtg tgcattaacg ccaacagtgc gatgaacccg
    23521 gtttttcagg gcaacggcaa gttgtgcgtc aacgtcctca accatgagca ggaactgatg
    23581 gcgcgccact tcgcgggcat gacaggcatg gcgatggaag agcgttttag cctctcatgc
    23641 tggcaaaaag gtccgctggc gcagccggtg ctaaaaggtt cgctggccag tcttgaagga
    23701 gagatccgcg atgtgcaggc gattggcaca catctggtgt atctggtgga gattaaaaac
    23761 atcatcctca gtgcagaagg tcacggactt atttacttta aacgccgttt ccatccggtg
    23821 atgctggaaa tggaagctgc gatttaagct ccagcccctt cacgcattcg tcgaaggggc
    23881 atttttattg tgatctcaat cacatatcta cccgatgaat aattcttgcc caaccgctca
    23941 ccgccattcc tgaccgctta catccctaaa ataaccactc agttatttac cttactttac
    24001 gcgcacgtat ctctggcaac atcactgcag gatagcggtc aatttacctc ctcaaacgca
    24061 acgcaaacct agaacggctt cggcctatga ttaacaaatt actctatgga ttttggtttg
    24121 caggaaggca gcaagtgagt gaatccccgg gagcttacaa aagtaagtgt cagaggtgaa
    24181 cgaacgcagc tgccgcacct gtaagccaaa agacgacgag taaaatgcca ggttttacta
    24241 tggatacgaa aaaactattc aagcacatac cctgggtgat tctcggaatc atcggtgcat
    24301 tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg
    24361 tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta cagtctgtac atcgcccaga
    24421 aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt tattaacaac gacggtctga
    24481 actacgttcc gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct atcgccggtg
    24541 ctggtccgct ggtgggaccg gttctcgccg cgcagatggg ctacctgcct ggcacgctgt
    24601 ggctgctggc gggggtagtg ctggccggtg cggtacagga ctttatggtg ctgtttatct
    24661 cctctcgccg taacggcgca tctctcggtg agatgatcaa agaagagatg gggccagtac
    24721 cggggactat cgcgctgttt ggctgtttct taatcatgat catcatcctc gccgttctgg
    24781 cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa
    24841 ccgtaccgat tgcgctgttt atgggtatct acatgcgctt tatccgtccg gggcgtgtgg
    24901 gtgaagtctc tgttattggt atcgtgctgc tggttgcctc tatctacttc ggtggcgtga
    24961 ttgctcacga tccgtactgg ggtccggcac tgacctttaa agacaccacc attaccttcg
    25021 cgctgattgg ctatgcgttt gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc
    25081 gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg
    25141 tggtgctgaa cccggaactg aaaatgcctg cgatgaccca gtacattgac ggtactggcc
    25201 cgctgtggaa aggcgctctg ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat
    25261 ctggcttcca cgcgctgatc tcttccggta cgacgccgaa actgctggct aacgaaaccg
    25321 acgcgcgttt cattggctac ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc
    25381 tggttgcagc gtccatcatc gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg
    25441 gccttggcat caccatgcct aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca
    25501 tggcgcagct gaaagacgtt accgcacacg cggcagcgac cgtcagctcc tggggcttcg
    25561 tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga
    25621 accgtgcagg tggtgcgccg acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc
    25681 tgccgatggc tgacatgggc ttctggtatc acttcggtat tctgttcgaa gccctgttca
    25741 tcctgaccgc gctggatgcg ggtacccgtt ctggccgctt tatgctgcaa gacctgctgg
    25801 gtaacttcat cccgttcctg aaaaaaaccg actctctggt tgccggtatc atcggtactg
    25861 cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg
    25921 gcgttaagag cctgtggccg ctgttcggta tctccaacca gatgctggca gccgtagcgc
    25981 tggtactggg caccgttgtg ctgattaaga tgaagcgcac ccaatacatc tgggtaactg
    26041 ttgttccggc tgtatggctg cttatctgca ccacctgggc gctgggtctg aaactgttca
    26101 gcaccaaccc gcagatggaa ggcttcttct acatggcaag ccagtacaaa gagaagattg
    26161 ctaacggtac tgacctgacg gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca
    26221 actacaccaa cgcaggcctg agtattctgt tcctgattgt ggtgtacagc atcatcttct
    26281 acggtttcaa aacctggctt gcggtgcgta acagcgacaa acgtactgac aaagaaacac
    26341 cgtacgttcc aatcccggaa ggcggcgtga agatctcttc gcaccactaa ccgtgtttag
    26401 ccccgcttcg gcggggcttt gttcttatca gagtgaacta tgtttggtaa cttaggacag
    26461 gcaaaaaaat atctcggcca ggcggcgaag atgttgattg gtattccaga ctacgacaac
    26521 tacgtcgagc atatgaagac caaccatccc gacaagccgt acatgagcta tgaagaattc
    26581 ttccgcgaac gccagaacgc gcgctacggc ggcgatggta aaggcggtat gcgctgttgt
    26641 taatggagag agcatgaacc cgattgcagt taccctactc accggttttt taggcgcggg
    26701 caaaactacc ctgctgcgcc atattcttaa cgaacaacac ggctacaaga ttgccgtgat
    26761 tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg attggcgacc gcgccacgca
    26821 gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc tccaacgagc tggaggacgc
    26881 actactcgac ctgctggata atctcgacaa gggcaatatt cagttcgatc gtctggtgat
    26941 tgaatgcacc ggcatggccg atcccggccc gattattcag acctttttct cccatgagat
    27001 tttatgccag ctctacctgc tggacggcgt gattgcgctg gtggatgcgg tacatgccga
    27061 tgagcagatg aaccagttca ccatcgccca gtcccaggtg ggctacgccg accgtattct
    27121 gctgactaaa accgacgtcg caggcgaagc agagaaactg cgtgaacgcc tggcgcgcat
    27181 caacgcccgc gcaccggtct acaccgtcac ccacggcgac atcgacctgg gcctgctgtt
    27241 caacaccaac ggttttatgc tggaagagaa cgtcgtcagc accaaaccgc gtttccactt
    27301 tatcgcggat aaacaaaacg atatttcgtc aattgtggtg gaactggatt acccggtaga
    27361 tatcagcgaa gtttcccgcg tgatggaaaa cctgctgctg gaatcggcgg ataaactgct
    27421 gcgttacaaa gggatgctgt ggattgacgg cgaacctaac cgcctgctgt tccagggcgt
    27481 ccagcgcctc tacagcgccg actgggacag gccgtggggc gatgaaaaac cgcatagcac
    27541 aatggtgttt atcgggattc agttgccgga ggaagagatt cgggcggcgt ttgcgggatt
    27601 gaggaagtaa gttcgggaaa gatcaacttt ttgagagttc caccgcaaca ttattacgag
    27661 tggaactctt tagatatgtc agaccaacca aagcttcttc aattgcgttt tcattttttc
    27721 atgtctcatt ttcagttcag ccaatcccca ttctgaagaa ttacaaacat cttttgttaa
    27781 ataaaaagag cttttactat taagtttctc ataaatagat ttctttttga taaaatcata
    27841 gtgttgtgtt tcagaatttt tggaaccact tatgagtgtt aaattaccta ttttatgcag
    27901 ccagtagaca tgctcttgag gttggaatct attaatccag tattcattaa ctaatgcttg
    27961 aggaagaata tgctcaattg tgattctccc cgtataggtt ttaatcacgc tctcatcttg
    28021 ttgctccata tctaagcgaa gcaaaattgc ttttattaag ttcacttgat tcggacgagg
    28081 ctcatataaa tcttcatcca gagcagctat aaaacctgaa ttatctgcat gttggtttat
    28141 ctgatttatc acagaatcaa aaggcatatc attatttatt gctaccagag cagaataaca
    28201 aaccatttct ctttggcttt taatttgttt ttttaaccac ccatgcatat ataccttctc
    28261 aaaggcggta ataaattgag aaaaatcatc taaattaaaa tcttctgtac gagccattcg
    28321 attcatgaat gccataaccg gaggaatcca ctcatctacc cctaaattac ttagcgatgc
    28381 tatttttcgt cgtattgaag ggtgttcaaa atcattctct aaaatcttag tata
//
LOCUS       DAJVRP010000051        28403 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVRP010000051 DAJVRP010000000
VERSION     DAJVRP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.051"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OQN12_002225"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OQN12_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCS1654532.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OQN12_002226"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OQN12_002226"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1654533.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OQN12_002227"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OQN12_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1654534.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OQN12_002228"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OQN12_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1654535.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OQN12_002229"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OQN12_002229"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1654536.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OQN12_002230"
     CDS             6426..7055
                     /locus_tag="OQN12_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1654537.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OQN12_002231"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OQN12_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS1654538.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8730..10073)
                     /gene="melA"
                     /locus_tag="OQN12_002232"
     CDS             complement(8730..10073)
                     /gene="melA"
                     /locus_tag="OQN12_002232"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS1654539.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OQN12_002233"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OQN12_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS1654540.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="OQN12_002234"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="OQN12_002234"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1654541.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="OQN12_002235"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="OQN12_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1654542.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="OQN12_002236"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="OQN12_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1654543.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="OQN12_002237"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="OQN12_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1654544.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="OQN12_002238"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="OQN12_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1654545.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="OQN12_002239"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="OQN12_002239"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1654546.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OQN12_002240"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OQN12_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1654547.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OQN12_002241"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OQN12_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1654548.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="OQN12_002242"
     CDS             complement(21746..22624)
                     /locus_tag="OQN12_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCS1654549.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OQN12_002243"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OQN12_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1654550.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25251..25586
                     /locus_tag="OQN12_002244"
     CDS             25251..25586
                     /locus_tag="OQN12_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1654551.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25744..26187
                     /gene="yjdN"
                     /locus_tag="OQN12_002245"
     CDS             25744..26187
                     /gene="yjdN"
                     /locus_tag="OQN12_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCS1654552.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26320..27108
                     /gene="phnC"
                     /locus_tag="OQN12_002246"
     CDS             26320..27108
                     /gene="phnC"
                     /locus_tag="OQN12_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1654553.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27133..28149
                     /gene="phnD"
                     /locus_tag="OQN12_002247"
     CDS             27133..28149
                     /gene="phnD"
                     /locus_tag="OQN12_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1654554.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28255..>28403
                     /locus_tag="OQN12_002248"
     CDS             28255..>28403
                     /locus_tag="OQN12_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCS1654555.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7296 a         7421 c         6934 g         6752 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agcactgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc accgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctgacagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac tagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgcaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctgg atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccgcgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgt atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 cgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctgc
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgca actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac caatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc attagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgtttttta tcagaataaa taacgtcttc tgatgcgttt aaaacgtcag aaagataaaa
    25021 atatcatgtg aataaaaaaa agaacaagta gagcattaac attatcttaa ataataaata
    25081 gaggcaaaaa gattattttc tttttacgtt tcctttcaaa tgaaaacgat cgtcgtctaa
    25141 aatcagcagt acccccgaca aactcaggga ttttgtgtat aattgcggcc tttttcggca
    25201 atctgccgtt ttttggcgct tttgccctgc tgacttttga ggaaatccac atgtcattac
    25261 cacactgccc aaaatgcaac tccgaataca cttacgaaga taacggcatg tacatctgcc
    25321 cggaatgtgc ctacgaatgg aacgacgcag aacctgcaca ggaaagcgac gagctgatcg
    25381 ttaaagatgc taacggcaat ctgctggctg acggcgacag cgttaccatc attaaagatc
    25441 tgaaggtgaa aggtagctct tcgatgctga aaattggcac caaagtgaaa aacatccgcc
    25501 tggttgaagg cgaccataac atcgattgca aaatcgacgg ttttggtccg atgaaactga
    25561 aatctgagtt tgtgaaaaag aactgattgt attgtgatcg gtaagccgga taaggcgctc
    25621 gcgccgcatc cggcaacgat gccgactgcc tgatgcgacg cttgcgcgtc ttatcaggca
    25681 gcctctcatc tgtataaatt tcgaactaca cttaactggc ttctcttaac tgaggtcacc
    25741 atcatgccgt taagtcccta cctctctttt gccggtaact gttccgacgc gattgcctat
    25801 tatcaacgta cgttgggcgc ggaactgctc tataaaatca gcttcggcga aatgccgaaa
    25861 tcagcgcagg acagcgccga gaactgccct tccggaatgc aatttcccga taccgccatc
    25921 gctcatgcca acgtgcgcat tgccggaagc gacatcatga tgagcgatgc tataccgtca
    25981 ggaaaagcca gctactccgg ctttacgctg gtgctcgatt cgcaacaggt cgaagaagga
    26041 aaacgctggt ttgataacct tgccgctaac ggaaaaatcg aaatggcctg gcaggaaact
    26101 ttctgggcgc atggctttgg caaagtcacc gataaatttg gcgtaccgtg gatgattaat
    26161 gtcgtcaaac aacaaccaac gcaataaccc gccgggaggc ccgccctccc gcactgtcat
    26221 cgaattcccg ttaactcttc atctgttagt cacttttaat taaccaaatc gtcacaataa
    26281 tccgccacga tggagccact tttttaggga ggctgcatca tgcaaacgat tatccgtgtc
    26341 gagaagctcg ccaaaacctt caatcagcat caagcgctgc atgcggttga tctgaacatt
    26401 catcacagtg aaatggtggc tctgcttggg ccgtcgggtt ccggcaaatc caccctttta
    26461 cgtcatttaa gcggtttgat taccggcgat aaatccgccg gtagccatat cgagctactg
    26521 ggccgcactg tccagcgcga aggccgcctg gcccgcgata tccgcaaaag ccgcgccaac
    26581 accggctaca tctttcaaca attcaacctg gtgaaccgcc tgagcgtact ggagaacgtg
    26641 ctgattggcg cgctcggcag cacgccgttc tggcgcacct gttttagctg gttcaccggc
    26701 gagcagaaac agcgcgcgtt acaggcgctg acccgcgttg gcatggtgca ttttgcccat
    26761 cagcgcgttt ccaccctctc cggcggccag cagcaacgtg tggcgattgc ccgtgcgctg
    26821 atgcagcagg cgaaagtgat tctggccgat gaacccatcg cctcgctgga cccggaatca
    26881 gcgcgcatcg tgatggacac cctgcgcgac atcaaccaga acgacggcat caccgtggtc
    26941 gtcacgctgc atcaggtgga ttacgccctg cgctactgcg aacgcatcgt cgccctgcgt
    27001 caggggcacg tcttctacga cggcagcagc caactgtttg ataacgaacg ttttgaccat
    27061 ctctaccgca gcattaaccg catcgaagag aacgcgaaag ctgcctgata tccccatcat
    27121 tgaggaaaac gaatgaacgc taagataatt gcctcgctgg ccttcaccag catgttcagc
    27181 ctcagcaccc tgttaacccc ggcacacgcc gaagagcagg aaaaggcgct gaatttcggc
    27241 attatttcaa cggaatcaca gcaaaacctg aaaccgcaat ggacgccgtt tttacaggat
    27301 atggagaaga aactgggcgt gaaggtcaac gccttctttg ccccggacta cgcgggcatt
    27361 atccagggga tgcgcttcaa taaagtggat attgcctggt acggcaacct gtcggcgatg
    27421 gaagcggtgg atcgcgccaa tggccaggtc ttcgcccaga cggtcgcggc ggatggatcg
    27481 ccgggttact ggagcgtgtt gatcgtcaac aaagacagcc cgatcaacaa cctgaacgat
    27541 ctgctggcga agcggaaaga tctcaccttc ggcaatggcg atcctaactc cacctctggc
    27601 ttcctcgtcc ccggttacta cgtcttcgct aaaaacaata tctccgccag cgacttcaag
    27661 cgcaccgtca acgccgggca tgaaaccaac gcgctggccg tcgccaacaa gcaagtggat
    27721 gttgccacca acaacaccga aaacctcgac aagctgaaaa cctccgcgcc ggagaagctt
    27781 aaagagctga aggtgatctg gaaatcgccg ctgatcctag gcgatccgat cgtctggcgc
    27841 aagaatctgt ccgaaactac caaagacaag atctacgact tctttatgaa ctacggaaaa
    27901 acgccggaag agaaagcggt gctggaacgc ctgggctggg cgccgttccg cgcttccagc
    27961 gacctgcaac tggtgccgat tcgccagctc gcactgttta aagagataca aagcgtgaaa
    28021 ggcaataaag ggctgaatga gcaggacaag ctggcgaaaa ccaccgagat tcaggcgcag
    28081 ctggatgacc tggaccgcct gaacaacgcg ttaagcgcga tgagttcggt gagtaaagcg
    28141 gtgcagtaaa tcgtaggtcg gataagacgc cccggcgtcg catccgacaa tgtgtaggcg
    28201 ttgatgccgg atgcggtgca agcaccttat ccggcctaca gaccggagcc aaacatgcaa
    28261 accatcacca tcgccccacc caagcgtagc tggttctcgc ttctgagctg ggccgttgtt
    28321 ctcgacgtgc tggtcgtctc gtggcagggc gcggaaatgg ccccgcttac gctgattaaa
    28381 gacggcggca acatggcaac ctt
//
LOCUS       DAJVRP010000052        28328 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVRP010000052 DAJVRP010000000
VERSION     DAJVRP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.052"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(6..1001)
                     /gene="yjfF"
                     /locus_tag="OQN12_002249"
                     /pseudo
     CDS             complement(6..1001)
                     /gene="yjfF"
                     /locus_tag="OQN12_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(988..2010)
                     /locus_tag="OQN12_002250"
     CDS             complement(988..2010)
                     /locus_tag="OQN12_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1654556.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2024..3526)
                     /locus_tag="OQN12_002251"
     CDS             complement(2024..3526)
                     /locus_tag="OQN12_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1654557.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3666..4622)
                     /gene="ytfQ"
                     /locus_tag="OQN12_002252"
                     /pseudo
     CDS             complement(3666..4622)
                     /gene="ytfQ"
                     /locus_tag="OQN12_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4932..5462
                     /gene="ppa"
                     /locus_tag="OQN12_002253"
     CDS             4932..5462
                     /gene="ppa"
                     /locus_tag="OQN12_002253"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1654558.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5841..6182)
                     /locus_tag="OQN12_002254"
     CDS             complement(5841..6182)
                     /locus_tag="OQN12_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1654559.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6185..9964)
                     /gene="tamB"
                     /locus_tag="OQN12_002255"
     CDS             complement(6185..9964)
                     /gene="tamB"
                     /locus_tag="OQN12_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1654560.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9961..11694)
                     /gene="tamA"
                     /locus_tag="OQN12_002256"
     CDS             complement(9961..11694)
                     /gene="tamA"
                     /locus_tag="OQN12_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1654561.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11900..12538
                     /gene="msrA"
                     /locus_tag="OQN12_002257"
     CDS             11900..12538
                     /gene="msrA"
                     /locus_tag="OQN12_002257"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1654562.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12861..14204
                     /locus_tag="OQN12_002258"
     CDS             12861..14204
                     /locus_tag="OQN12_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1654563.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14266..14472)
                     /locus_tag="OQN12_002259"
     CDS             complement(14266..14472)
                     /locus_tag="OQN12_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1654564.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14797..15351
                     /locus_tag="OQN12_002260"
     CDS             14797..15351
                     /locus_tag="OQN12_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1654565.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15414..16352)
                     /locus_tag="OQN12_002261"
     CDS             complement(15414..16352)
                     /locus_tag="OQN12_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654566.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16564..17304)
                     /gene="cysQ"
                     /locus_tag="OQN12_002262"
     CDS             complement(16564..17304)
                     /gene="cysQ"
                     /locus_tag="OQN12_002262"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1654567.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17494..19437
                     /gene="cpdB"
                     /locus_tag="OQN12_002263"
     CDS             17494..19437
                     /gene="cpdB"
                     /locus_tag="OQN12_002263"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1654568.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19563..19943)
                     /locus_tag="OQN12_002264"
     CDS             complement(19563..19943)
                     /locus_tag="OQN12_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1654569.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20032..20892
                     /gene="qorB"
                     /locus_tag="OQN12_002265"
     CDS             20032..20892
                     /gene="qorB"
                     /locus_tag="OQN12_002265"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1654570.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21001..21966
                     /locus_tag="OQN12_002266"
     CDS             21001..21966
                     /locus_tag="OQN12_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1654571.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22074..22736
                     /gene="ytfE"
                     /locus_tag="OQN12_002267"
     CDS             22074..22736
                     /gene="ytfE"
                     /locus_tag="OQN12_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1654572.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22781..24193)
                     /gene="cycA"
                     /locus_tag="OQN12_002268"
                     /gene_synonym="dagA"
     CDS             complement(22781..24193)
                     /gene="cycA"
                     /locus_tag="OQN12_002268"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1654573.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24501..25121)
                     /gene="fklB"
                     /locus_tag="OQN12_002269"
     CDS             complement(24501..25121)
                     /gene="fklB"
                     /locus_tag="OQN12_002269"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1654574.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25340..25885
                     /locus_tag="OQN12_002270"
     CDS             25340..25885
                     /locus_tag="OQN12_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCS1654575.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25869..26516)
                     /locus_tag="OQN12_002271"
     CDS             complement(25869..26516)
                     /locus_tag="OQN12_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1654576.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26728..27519
                     /locus_tag="OQN12_002272"
     CDS             26728..27519
                     /locus_tag="OQN12_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCS1654577.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27529..>28328
                     /locus_tag="OQN12_002273"
     CDS             27529..>28328
                     /locus_tag="OQN12_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCS1654578.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6950 a         7539 c         6984 g         6855 t
ORIGIN      
        1 catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg attctcccac
       61 agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc gatggcgatt
      121 ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat cagtccctga
      181 attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa caacgtaccg
      241 ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag tgcatatccg
      301 gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt ggagagcata
      361 taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga cgtcgcgttg
      421 ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa aataccgata
      481 accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc ggggattttc
      541 cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga ctcttccgaa
      601 acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat aaatgccggg
      661 atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc catcaccagc
      721 actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt tgccagaaac
      781 acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga gaggatcaca
      841 aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat attgcagatc
      901 acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc cagcacaaag
      961 acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta ctcctttaat
     1021 cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca ccgctttcac
     1081 cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca tcccctgaat
     1141 aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca gcgatccgcc
     1201 gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt tgttggcatc
     1261 ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata gcccgctcaa
     1321 cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg ccgcccgaat
     1381 gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca acagccagaa
     1441 caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca gcgatccgct
     1501 gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg tgatcagttg
     1561 cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct gaattttgag
     1621 gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca gggcgcttaa
     1681 caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg ctccggcgat
     1741 agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca gcgtcatacc
     1801 aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc taccgaacaa
     1861 acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat cgaccagcag
     1921 caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac gcctttttgg
     1981 cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc gcaatggcgt
     2041 tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt ttgcgatcgc
     2101 gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag gagatcacca
     2161 gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg gcgtgggcac
     2221 caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg gtcagtagcc
     2281 aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc ggttgttcag
     2341 ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct tgctgctctt
     2401 tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg atattttccc
     2461 gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag aatccgatgc
     2521 ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg atcaacgccg
     2581 tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta cgtccggatc
     2641 ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca aacggtgcta
     2701 tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc aacaatgttc
     2761 gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt tttaccagtt
     2821 cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg cgtaagacgg
     2881 tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag atcaggctga
     2941 cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc tgggtgtcga
     3001 ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata gcccggcaaa
     3061 tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc acgtcgaggg
     3121 agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta cggcgtagaa
     3181 ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac atattgggta
     3241 gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg gcggtatttt
     3301 tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg tatacaccag
     3361 ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc gccatgattt
     3421 cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg aaaaatttac
     3481 ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt tcccccggtg
     3541 taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag gcttcagtgc
     3601 accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat caggcatagc
     3661 aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt atcaggcagg
     3721 taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt tttgtatttc
     3781 tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact ggcgttcgct
     3841 tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc cgtgaggata
     3901 tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat caccatgtcg
     3961 tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt gataaagctc
     4021 tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg gataattttg
     4081 atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat ggcgacgctg
     4141 gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac ttcttttacc
     4201 agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt ggtcatatag
     4261 agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat ttcggcatct
     4321 ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat aaagatcgca
     4381 tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg cttttgctga
     4441 ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc cacattggtt
     4501 tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt taatggagcg
     4561 gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa gcgtttccac
     4621 atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg aaaaaactat
     4681 agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta aataaaacgt
     4741 taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac ggttatggat
     4801 tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga cgaaaacaag
     4861 caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac atatttttaa
     4921 aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg gaagacatct
     4981 acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc gacaaagaga
     5041 gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg tgcaactacg
     5101 gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg gtcccgactc
     5161 cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt ctgaaaatga
     5221 ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag ctgagcaaag
     5281 aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg caaatcgctc
     5341 acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt gaaggttggg
     5401 aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca aagaataaat
     5461 aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg tttttttatg
     5521 cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc aaggcttgaa
     5581 cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg ctggaagtta
     5641 gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct gcacatttgt
     5701 gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg agagaaaagc
     5761 aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca acgccacccg
     5821 aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct ttcaattagc
     5881 tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata cggcgtctga
     5941 atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc ggccagcgtg
     6001 gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac tgcgcctgga
     6061 tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag taactgggca
     6121 ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac aaatattcgc
     6181 attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca gacacggctt
     6241 ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata gagtcaaata
     6301 tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc actacctggg
     6361 aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac gtctcgccga
     6421 ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc gaggtcattg
     6481 ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa gacaatgcag
     6541 cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc gccagaccag
     6601 tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga atagcttcaa
     6661 tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta cgcacaatca
     6721 gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg ttcaggccca
     6781 gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc aggccaaagg
     6841 cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc ggaatcgacg
     6901 ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg ctggagacgc
     6961 ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca tccacgcgac
     7021 catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat acatccattc
     7081 gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact cgtgcccgcc
     7141 agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc tgctgggtcc
     7201 gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc tggctcggct
     7261 gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga agctgaccaa
     7321 acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc atccccgctg
     7381 ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta cggatattga
     7441 cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt ccatcaaact
     7501 ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta cgcaattccg
     7561 ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg gtttgcgtta
     7621 cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct ttcgtggtgt
     7681 cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt tctggcataa
     7741 acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc ccttcggccc
     7801 ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag cagtgtggcc
     7861 cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg gtcagcgacc
     7921 acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag cgttcttctt
     7981 tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc tgaatccgca
     8041 actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg ttgatattta
     8101 catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc tgatcggtgg
     8161 atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag cgcaggccgc
     8221 gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact ttcaccagcc
     8281 ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg ttgatggtgg
     8341 catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg tttggcccga
     8401 gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc agcgtgccgt
     8461 caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc acctccatct
     8521 gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt ttcgacggcc
     8581 actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg cgccaactaa
     8641 tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc gccgcgacgg
     8701 tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta atggtggctg
     8761 gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg gtcattttgc
     8821 cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg aacggccagt
     8881 aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc agtcgcgtct
     8941 gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc agctgttcgc
     9001 gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc acgttcagtg
     9061 tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg ccgctggcgt
     9121 tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg ccgtcaatgc
     9181 tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc acgcgcagct
     9241 gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg gtcatttccg
     9301 gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt tcctccggct
     9361 gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc actttcggca
     9421 gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc tcctgccagt
     9481 tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg atgttgacgt
     9541 tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc agcggaccgc
     9601 tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg atgttgacct
     9661 gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg cactcaagac
     9721 cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca taacgaacgt
     9781 cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt cctggcaccc
     9841 agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc agaaacgcca
     9901 ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt ttccataaac
     9961 tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt tatccgcgac
    10021 cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta cgccgacccc
    10081 ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac tatcgacaaa
    10141 caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc cggttatcaa
    10201 cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt tgtatttgta
    10261 gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta ctttgtcgaa
    10321 atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc gatcgtacag
    10381 tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg aaccccaggc
    10441 cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca tcaggccacc
    10501 acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg tgttggtaat
    10561 ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct gccagccgct
    10621 ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg tatcgttcag
    10681 gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat tcttcagcag
    10741 cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg tactggtggt
    10801 caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca cgcgcggtcc
    10861 cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg gcgaaaccac
    10921 gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag ccaccaccac
    10981 cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat ctttcgattc
    11041 atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc ggatttgtga
    11101 tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat caatatccca
    11161 gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt cgctatcgaa
    11221 ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat aatcgccctg
    11281 gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt ctttatcggt
    11341 ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca cacctggcgt
    11401 gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat caaattcaat
    11461 ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat cgacgcgtgc
    11521 gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct gcgcacgaac
    11581 gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg cggcgacggc
    11641 agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc gcacattttc
    11701 tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac aaaaatccag
    11761 taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac tttagaccta
    11821 ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc caatgaccga
    11881 ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt cccccgccga
    11941 tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg tcaacggtca
    12001 ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg gttgtttctg
    12061 gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg caggctatac
    12121 cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata cgggtcatgc
    12181 cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt tgctacaggt
    12241 attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc acggcacgca
    12301 gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc gcgccagtct
    12361 ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca ccacggaaat
    12421 cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc tgcataaaaa
    12481 cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg aagcatagcg
    12541 ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg atgcggctta
    12601 aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga ctgcgtaggc
    12661 ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat tttaaccctc
    12721 tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt cactttcttc
    12781 gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc gagggtctgg
    12841 cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc ttgatcgctg
    12901 taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa atcaaactta
    12961 aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg caggaaaatc
    13021 ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt ctcggcggta
    13081 tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc tatatgtcgg
    13141 cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact ggcatgttta
    13201 tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa gcggtggctt
    13261 tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg gtgtggttct
    13321 tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta cgtaaagatg
    13381 acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg ggcgtgttac
    13441 gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt accgttccgt
    13501 cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat gagcaaagcc
    13561 tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat gaagatattg
    13621 accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg gctaaccaaa
    13681 gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg gatacgttaa
    13741 ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg gtgatcatga
    13801 acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc acgctgatgg
    13861 gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag aactcatggc
    13921 tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt gacgagttcc
    13981 cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg cgtaagatcc
    14041 cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg gatatcgata
    14101 actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc gccctttcgc
    14161 caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca gaaacatcaa
    14221 cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag ccatctctgt
    14281 ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca gcttatcctt
    14341 cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt ccttgatgta
    14401 taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt tgtaccgttg
    14461 gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca caatcggccc
    14521 aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg gcaatgtttc
    14581 caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc tttttaccga
    14641 agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg accgcaaact
    14701 ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc agacccaagt
    14761 ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa gattctggca
    14821 ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat cggtcagcga
    14881 gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga tcagtttagc
    14941 tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca acatattgct
    15001 ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa atcagcgaag
    15061 ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc gattccgggt
    15121 tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc ctggtcgcag
    15181 tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga agaaagttcc
    15241 gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg ggcgctcaca
    15301 ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata atggcaataa
    15361 cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg gaattaagtc
    15421 tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt aaacgcctca
    15481 atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc tgcggccaga
    15541 ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca acctgcatta
    15601 accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga ggaaccatta
    15661 cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc tatgactatg
    15721 gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc tttaattcca
    15781 ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac atcctccaat
    15841 aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt aatccttttt
    15901 ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct gttttgttct
    15961 aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat tgttatggga
    16021 atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc accgttatct
    16081 ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt ttttgatgaa
    16141 agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc ccgcaaagtt
    16201 aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg aatattatta
    16261 aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc caccgcacca
    16321 ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat ttggttattt
    16381 tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct cattattaaa
    16441 atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt tatttataac
    16501 tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt ctgccatctg
    16561 aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg gagtgtaatc
    16621 cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca cagcatgtcc
    16681 ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg cctgtccttc
    16741 cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt cgccaagctg
    16801 ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca ccagcggcgg
    16861 gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc aggccttgcc
    16921 ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta aaatcggttt
    16981 gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa taaactcttt
    17041 agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga cttcccaacc
    17101 gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta aaccgtccat
    17161 gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg ctttgctgac
    17221 gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg cattccgtgc
    17281 aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt gttaacttat
    17341 tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc atcattttca
    17401 aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt ctcccttttc
    17461 tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc aacgctcctg
    17521 gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat catggaaacc
    17581 actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc cacggaaaaa
    17641 ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt gaaaaacagc
    17701 gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta catgtcggcg
    17761 aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac gctggattat
    17821 acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct gaaaaatgcg
    17881 ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag aaccaaacag
    17941 ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga cggaaaaaaa
    18001 cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg ctgggataaa
    18061 gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg caaatacgtg
    18121 cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc cgggctgtct
    18181 gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga aattccgggc
    18241 gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga ttttgctgat
    18301 atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt aatgccgggc
    18361 atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag cggtaaatgg
    18421 caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa taaaaaatcc
    18481 ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga tgccacacgc
    18541 cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta tctggcgctg
    18601 gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta tgtcgaacat
    18661 tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc cgcaccgttt
    18721 aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa aggccagttg
    18781 accttccgta atgctgccga tctttatctc taccccaata cgctgattgt agtgaaagcc
    18841 agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa ccagattgct
    18901 cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac ttataacttt
    18961 gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg ctatgacggc
    19021 gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt taatggcaag
    19081 ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc ttacggcggc
    19141 aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga gaaccgctcg
    19201 gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca tccggcggca
    19261 gataacaact ggcgtttagc accgatagct gccgataaga aactggatat ccgtttcgaa
    19321 acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta tccgatgaat
    19381 aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag taagtaaaac
    19441 acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat gcgatgctgc
    19501 cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag cgcatccggc
    19561 agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa ctcaatccag
    19621 tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag gctatattcc
    19681 acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg ttctaacgcc
    19741 tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg caggtcgcta
    19801 aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg gctggtgacg
    19861 tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc tttcagttgt
    19921 tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt actaaaagta
    19981 agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc catgatcgct
    20041 attactggtg ccactggcca acttggtcac tatattattg aatccttgat gaaaacggtt
    20101 cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc cctggcagca
    20161 caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac atctgcactt
    20221 cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg tgccccgcag
    20281 catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc ttataccagc
    20341 ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga gacggagaaa
    20401 atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta cagcgaaaac
    20461 tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc ggcgggcgat
    20521 ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg cgtgattagc
    20581 gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg gacgttgaca
    20641 cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca aaatctgagc
    20701 gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact ggcggatatg
    20761 ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag caaaacgctt
    20821 agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca tctttttaat
    20881 gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa tgacgggatg
    20941 ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg gatgccagta
    21001 atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct tatttttgtc
    21061 gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg ttatctggcg
    21121 ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg tcagttggcg
    21181 cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat ttattacttc
    21241 tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat tatcggcacc
    21301 ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga cggcaaactc
    21361 gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc gagtgtgaat
    21421 attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac ctctggcatc
    21481 gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa cgcccgctgg
    21541 ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc gctggtcacg
    21601 ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat acaaacgccg
    21661 gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat ggttgcgata
    21721 gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca gcgattaccg
    21781 acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct gtacaccttt
    21841 ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct gttagtgatt
    21901 ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc tgtctcagaa
    21961 agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta tagcttttgc
    22021 cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca gctatggctt
    22081 atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg gctctgtttc
    22141 gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc gcggcggcac
    22201 gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct gaacaaccga
    22261 ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc atcgtgcgct
    22321 accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact aaagtcgagc
    22381 gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg accatgctgc
    22441 atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg atgatcaaac
    22501 aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag cacgatgaag
    22561 cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg ccgccagaag
    22621 cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat gacctgatgg
    22681 atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt gagtgatcta
    22741 aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc agttcagcag
    22801 cccgcttctt accaataaac agccagccca accccagcgc gataaaccac agcggggtaa
    22861 ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg aacacaaaga
    22921 acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc gacttttcat
    22981 gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc gtccagacga
    23041 acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc acgctaggat
    23101 tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc aaccctttcg
    23161 cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct tcctgcgcca
    23221 gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac gccgcagagg
    23281 tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac acaaacagct
    23341 caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac ataatcacaa
    23401 tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc gcgcgtggca
    23461 gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca atccccacga
    23521 aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc gggaaccagc
    23581 cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac tgaaagtgca
    23641 tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt ttgatcatcg
    23701 caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc gtcagcagca
    23761 gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag aactgagcat
    23821 aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac cagtaagtcc
    23881 agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca ctaaaagatt
    23941 tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa aagagcataa
    24001 aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc gttttgccgg
    24061 accccataaa caacccagta ccaatggcac ctccaatggc aataagctga acatgtcggt
    24121 ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca acgactttta
    24181 cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct ctacggcctt
    24241 tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg taacaatacg
    24301 cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg agtgtcatat
    24361 cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt tgcaacacaa
    24421 aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc ctctggttcg
    24481 aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa caccagggtg
    24541 ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc ctgcgggata
    24601 gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca gccagggatc
    24661 acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc aaacacggtg
    24721 ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc cggaattgcg
    24781 ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt cacaccttct
    24841 tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg gaaacgctga
    24901 cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac cacatcaacc
    24961 ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag tgcttctggc
    25021 agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc aatgccgtag
    25081 cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc tttcttaggg
    25141 tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt gtcgcaggaa
    25201 tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat ataataaagg
    25261 cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg aggaacaaga
    25321 cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc tggagaaagt
    25381 ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc atcgtcgggg
    25441 gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg atgatacgcc
    25501 caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat cacaaccgcc
    25561 gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata gcggaattga
    25621 taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc tgttccgcga
    25681 ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg cgggtaagcc
    25741 gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca gcggcgtggt
    25801 gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc gccagccgga
    25861 tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct aaggattgtc
    25921 tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact gatatgataa
    25981 actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc aatattaata
    26041 ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc ttcatccaat
    26101 aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg tgattttgca
    26161 taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca aggattagca
    26221 atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg cggaagatct
    26281 gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc cagcacttcg
    26341 ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact acaccctgct
    26401 gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa ttcattaacc
    26461 gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat atacataacg
    26521 cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag cttcagaaaa
    26581 aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc catcgcatat
    26641 caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat caccaaggaa
    26701 acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc cgtgattacc
    26761 ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg cgggtttaac
    26821 ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat cacacaacaa
    26881 gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg tgatctatct
    26941 ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga ctgtctgctc
    27001 aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga accagacagc
    27061 ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca ggcattcagt
    27121 aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc gattattttc
    27181 gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac cattgccaaa
    27241 accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga acaaattggc
    27301 gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc aacggcttat
    27361 tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta tccggctgat
    27421 attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg tggtcaggcg
    27481 gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat gagagatctg
    27541 ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa aacgccgcta
    27601 cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc ctggcgcagg
    27661 gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag taacatgagc
    27721 gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga acgcaaactg
    27781 gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc cgcctgttat
    27841 atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc gcgagaacaa
    27901 gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt gccaattgcg
    27961 ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac gttacgtcag
    28021 gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg cgtggcggtg
    28081 gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg tgccggaaaa
    28141 cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct ggtcaatgac
    28201 cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct ggttgaaaac
    28261 gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg gcagaaagat
    28321 attaacag
//
LOCUS       DAJVRP010000053        27601 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVRP010000053 DAJVRP010000000
VERSION     DAJVRP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.053"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OQN12_002274"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OQN12_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1654579.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OQN12_002275"
     CDS             complement(1614..1937)
                     /locus_tag="OQN12_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1654580.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQN12_002276"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQN12_002276"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1654581.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQN12_002277"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQN12_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1654582.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQN12_002278"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQN12_002278"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1654583.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQN12_002279"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQN12_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1654584.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OQN12_002280"
     CDS             7537..8574
                     /locus_tag="OQN12_002280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1654585.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQN12_002281"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQN12_002281"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1654586.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OQN12_002282"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OQN12_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1654587.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQN12_002283"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQN12_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1654588.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OQN12_002284"
     CDS             10745..11626
                     /locus_tag="OQN12_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1654589.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQN12_002285"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQN12_002285"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1654590.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OQN12_002286"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OQN12_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1654591.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQN12_002287"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQN12_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OQN12_002288"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OQN12_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1654592.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OQN12_002289"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OQN12_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1654593.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OQN12_002290"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OQN12_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1654594.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OQN12_002291"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OQN12_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1654595.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OQN12_002292"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OQN12_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1654596.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OQN12_002293"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OQN12_002293"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1654597.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OQN12_002294"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OQN12_002294"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1654598.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OQN12_002295"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OQN12_002295"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1654599.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OQN12_002296"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OQN12_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1654600.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OQN12_002297"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OQN12_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1654601.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQN12_002298"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQN12_002298"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1654602.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OQN12_002299"
     CDS             24893..25273
                     /locus_tag="OQN12_002299"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1654603.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OQN12_002300"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OQN12_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1654604.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQN12_002301"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQN12_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1654605.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="OQN12_002302"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="OQN12_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6812 a         6867 c         7233 g         6689 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgaggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtacgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAJVRP010000054        27546 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVRP010000054 DAJVRP010000000
VERSION     DAJVRP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.054"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQN12_002303"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQN12_002303"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1654606.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQN12_002304"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQN12_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1654607.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OQN12_002305"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OQN12_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1654608.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OQN12_002306"
     CDS             3807..3995
                     /locus_tag="OQN12_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1654609.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OQN12_002307"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OQN12_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1654610.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OQN12_002308"
     CDS             4756..5115
                     /locus_tag="OQN12_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCS1654611.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OQN12_002309"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OQN12_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OQN12_002310"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OQN12_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS1654612.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OQN12_002311"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OQN12_002311"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1654613.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10895..11071
                     /locus_tag="OQN12_002312"
                     /pseudo
     CDS             10895..11071
                     /locus_tag="OQN12_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            11399..13147
                     /gene="bcsC"
                     /locus_tag="OQN12_002313"
                     /pseudo
     CDS             11399..13147
                     /gene="bcsC"
                     /locus_tag="OQN12_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            13229..15217
                     /gene="hmsP"
                     /locus_tag="OQN12_002314"
     CDS             13229..15217
                     /gene="hmsP"
                     /locus_tag="OQN12_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1654614.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            15402..16688
                     /gene="dctA"
                     /locus_tag="OQN12_002315"
     CDS             15402..16688
                     /gene="dctA"
                     /locus_tag="OQN12_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1654615.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            16909..18405
                     /locus_tag="OQN12_002316"
     CDS             16909..18405
                     /locus_tag="OQN12_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1654616.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(18501..19430)
                     /gene="kdgK"
                     /locus_tag="OQN12_002317"
     CDS             complement(18501..19430)
                     /gene="kdgK"
                     /locus_tag="OQN12_002317"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1654617.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            19662..20429
                     /gene="pdeH"
                     /locus_tag="OQN12_002318"
     CDS             19662..20429
                     /gene="pdeH"
                     /locus_tag="OQN12_002318"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1654618.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            20499..22559
                     /locus_tag="OQN12_002319"
     CDS             20499..22559
                     /locus_tag="OQN12_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1654619.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(22741..24063)
                     /locus_tag="OQN12_002320"
     CDS             complement(22741..24063)
                     /locus_tag="OQN12_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1654620.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(24474..25487)
                     /gene="yhjD"
                     /locus_tag="OQN12_002321"
     CDS             complement(24474..25487)
                     /gene="yhjD"
                     /locus_tag="OQN12_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1654621.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(25536..26435)
                     /locus_tag="OQN12_002322"
     CDS             complement(25536..26435)
                     /locus_tag="OQN12_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1654622.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            26955..>27546
                     /locus_tag="OQN12_002323"
     CDS             26955..>27546
                     /locus_tag="OQN12_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1654623.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLN"
BASE COUNT         6565 a         6946 c         7551 g         6484 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccaag
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaataatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgtgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caactctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtagtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactttcgc aggatctctg gcaggccgga caacgcagcc aggccgatac
    11221 gttaatgcgc aatctggcgc agcagaagcc gaacaacccg gagcaggttt acgcttacgg
    11281 gctgtacctc tcaggtcaca accaggacag agcggcgctg gcgcatatca acagcctgcc
    11341 gcgcgcgcag tggaacagca atattcagga gctggttaat cgactgcaaa gcgatcaggt
    11401 gctggaaacc gctaatcgcc tgcgagaaag cggcaaagag gctgaagcgg aagcgatgct
    11461 gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg cgtaacaacg
    11521 acgtgattac accgccgccc gcactgcata tcagaatgtc ctgacgcggg agccaactaa
    11581 cgccgatgcc attcttggtc tgacggaagt ggatattgct gccggtgaca aagcggcggc
    11641 acgtagccag ctggcgaaac tgcctgccac cgataacacc tcgctgaaca ctcagcggcg
    11701 cgtggcgctg gcacaggcgc agcttggaga taccgcagcg gcgcagcaga cgtttaataa
    11761 gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg tactgcgcga
    11821 cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag gcgctggaaa cctacaaaga
    11881 cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca cctttacccg
    11941 actgacccgt aacgacgaga aagatgactg gctgaaacgc ggcgtgcgca gcgatgcggc
    12001 ggacctctat cgccagcagg atcttaacgt cacccttgag cacgattact ggggttcgag
    12061 cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg tggatgcgcc
    12121 gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg tcggcagttt
    12181 ctccactaat gccgatggca aatgggatga caactggggc acctgtacat tacaggactg
    12241 tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct ggcgaaatga
    12301 cgtctggagc tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg atgtggtcgg
    12361 cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg cccaccgtcg
    12421 gcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga gcaataccgg
    12481 gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta agtctgagct acgataaagg
    12541 tgaagtaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta aaaatgtcga
    12601 agataactgg cgcgtgcgct ggatgacggg ctattactat aaggtcatta accagaacaa
    12661 tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag atctgagtgg
    12721 ctactcactc ggtcagggcg gttactacag tccgcaggaa tacctgtcgt ttgccatacc
    12781 ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt ctggctcgtg
    12841 gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga tcccgaccga
    12901 ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg gcttcggcta
    12961 cacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgttg gtacggcaat
    13021 tgatatccag caggcgaaag attacgcacc cagccatttc ctgctctacg tacgttattc
    13081 cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac cttacgccga
    13141 ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat actcgggcgg
    13201 caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa caatcaagca
    13261 gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca ccgttttgct
    13321 gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa gcattgctcg
    13381 ctctgtccgc gaacccttat cttcagctat tttgaaaggc gatattcccg aagcggaagc
    13441 tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag tgctgcctaa
    13501 ccagttccag gcgctgcgta aaagttttat tccagagcgt ccggtgccgg taatggttac
    13561 tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtc tactcgctcg aacgtccggc
    13621 aaatccgcag ccaatagcct atctggtgct acaggcggat tccttccgta tgtataagtt
    13681 cgtgatgagc actctctcaa cgttagtgac catttactta cttttgtcgc ttatcctgac
    13741 cgtcgccatc agctggtgca ttaaccgcct gattttgcat ccgttacgca atattgctcg
    13801 cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat taccgcgtct
    13861 gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc agcaattgct
    13921 gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt cggatttgcc
    13981 gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac aaaccaccgc
    14041 gctgatgatc atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga aagaggcgca
    14101 acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt cgccacgtat
    14161 gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg tacaggaacc
    14221 gtggcacgca atcactttgg gtcagcaagt actcactatc atgagcgagc gcctgccgat
    14281 tgaacgtatt caactccgtc cgcactgtag cattggcgtg gcgatgttct acggcgatct
    14341 caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc gccataaagg
    14401 caagaatcag attcagttct ttgatccgca gcagatggaa gcagcccaga agcggttgac
    14461 ggaagagagc gatatcctta atgcactgga aaatcatcag tttgcaattt ggttacagcc
    14521 acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc gtatccagca
    14581 accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt gctgtgggct
    14641 gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg cagcctggca
    14701 agagcgcggc attatgctgc ccttgtcggt aaacttctct gcgctgcaac tgatgcaccc
    14761 gaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc cgggaacact
    14821 gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg tggcaatcct
    14881 ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat gatttcggca tgggctacgc
    14941 agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgtactga aaatcgacaa
    15001 aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta tcatgctggc
    15061 gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac aacgagactg
    15121 gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcgcc cactccctat
    15181 tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg attacaaaac
    15241 tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt gtttcaatta
    15301 tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc aggtaccccc
    15361 cataacctta caagacctgt ggttttacta aaggacaccc tatgaaaacc tctctgttta
    15421 aaagccttta ctttcaggtc ctgacagcga tagccattgg tattctcctt ggccatttct
    15481 atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg cttcgttaag ctcattaaga
    15541 tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg cattgcgggc atggaaagca
    15601 tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta ctttgaaatt gtcagtacca
    15661 tcgcgctgat tattggtctt atcatcgtta acgtcgtgca gcctggtgcc ggaatgaacg
    15721 tcgatccggc aacgcttgat gcgaaagcgg tagcggttta cgccgatcag gcgaaagacc
    15781 agggcattgt cgccttcatt atggatgtca tcccggcgag cgtcattggc gcatttgcca
    15841 gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt tggttttgcg ctccaccgtc
    15901 tgggcagcaa aggccaactg atttttaacg tgatcgaaag tttctcgcag gtcatcttcg
    15961 gcatcatcaa tatgatcatg cgtctggcac ctattggtgc gttcggggca atggcgttta
    16021 ccatcggtaa atacggcgtc ggcacactgg tgcaactggg gcagctgatt atctgtttct
    16081 acattacctg tatcctgttt gtggtgctgg tattgggttc aatcgctaaa gcgactggtt
    16141 tcagtatctt caaatttatc cgctacatcc gtgaagaact gctgattgta ctggggactt
    16201 catcttccga gtcggcgctg ccgcgtatgc tcgacaagat ggagaaactc ggctgccgta
    16261 aatcggtggt ggggctggtt atcccgacag gctactcgtt taaccttgat ggcacatcga
    16321 tatacctgac aatggcggcg gtgtttatcg cccaggccac taacagtcag atggatatcg
    16381 tccaccaaat cacgctgtta atcgtgttgc tgctttcttc taaaggggcg gcaggggtaa
    16441 cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc ggtgggccat ttgccggtag
    16501 cgggtctggc gctgatcctc ggtatcgacc gctttatgtc agaagctcgt gcgctaacta
    16561 acctggtcgg taacggcgta gcgaccattg tcgttgctaa gtgggtgaaa gaactggacc
    16621 acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga tggcaaaacg cacgaattat
    16681 cctcttaatc tcaccactta tgcccgcact cccttccccg agtgcgggtg tttgcgcata
    16741 attgacccct gtgcctgaac ttaccacctg ggtgagttca ggtttatttc acttcttccg
    16801 tgacattttc atgttcttgc ggtctaacac gaagtgtttt taacgtcatt tagactgacc
    16861 gacaacgggc agttttttta ttaccaggat agttgatcag gggttcacat gcagggcata
    16921 aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta tgtgcaggca
    16981 gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg tttacagtgg
    17041 caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct gctggttaat
    17101 accggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat ccctcgtatt
    17161 gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg gcagcagggg
    17221 atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac gctgtttaat
    17281 ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta tctggcaaat
    17341 gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca aagtcaggac
    17401 atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg tctgaaaggg
    17461 tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga agcggaaaaa
    17521 attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact ggtggtggga
    17581 aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga actgaaaggc
    17641 aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt
    17701 attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac gccgtggcag
    17761 ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc ccgcgaggcg
    17821 ctgttctggc atgttcagca agcgttaagc gccagtgaca gcaaagacat cggtcttgga
    17881 tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac
    17941 gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa
    18001 ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga gctgcagaaa
    18061 ctgtttgccg cctatgcacg ggctgatacc gatattctga tgggccagcg gatgcgttcg
    18121 ttgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg gcaggatttc
    18181 cttaacagcc tgactgtgga gatgttaaat caggatctgc gtcagcagtt gtcgaatgat
    18241 atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa agcgttgcag
    18301 gcggcctggg atcaaatcat ggccccatct accgccgctg cgaccacctc tgtcgccacg
    18361 gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct gatggtgatg
    18421 agttagcaca accatcaggt atttatggat gagctggata gaggggtaac actttatcca
    18481 gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat cgcgccgcga
    18541 tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc cgcactgccg
    18601 cccgtcaaac gtaccgccag ataaccggcg ctgaaagagt cgccagctgc ggtggtatcg
    18661 atcacttttt ctttcggcag tttcaccgcc ggaacatcaa ctaacgcttc accagcaatg
    18721 gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc cgcgttatgg
    18781 gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc gtcgtccagc
    18841 gtcaggaagg cgatatccgt gcattccagc atttgctggt acacctgctg tgtctcttct
    18901 ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc gcctttggcg
    18961 cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg
    19021 ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat cgccgcagac
    19081 tgctcgctcg ccagccagaa tttggcggcg gcttcgttac gccagtagta gaacgtacgt
    19141 tcgccggtgc tgtcggtttc aatgtagtaa aggcccggaa gacggttttc catccgttgg
    19201 gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatctgctg gctaaaactg
    19261 tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac ctgacgggcg
    19321 atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc cgcgcctttc
    19381 tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat agtcgtgaac
    19441 ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc aaccatatta
    19501 aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc cgttgtggag
    19561 tgaggaaatt taccccggcc aggcggtaaa gttctgccct tacgcgccga taatctttgt
    19621 cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg caggttatcc
    19681 agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt ttttggttgc
    19741 agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcgggcgg ttaatggccg
    19801 tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg ccgccggatc
    19861 gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag cagattgatt
    19921 tgctggcgca aaaagccgac ttctttgtag agcacggcct gctggcatcg gtcaatattg
    19981 atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccag attgagcgtc
    20041 ttccctggct gcgtttcgaa ctggtggagc atatccgtct gccgaaagat tcaacctttg
    20101 cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggtaccggg atggcaaatt
    20161 tctctgcact aagtgaagtg cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga
    20221 tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat ctaatgaatc
    20281 gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg cgtgatgttc
    20341 agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca ccgatggaaa
    20401 cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgccggg ctggactatc
    20461 tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca ggcaaaataa
    20521 ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa
    20581 cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg gagttgaatc
    20641 gtccgttcgc tatccgtggc aatctgggcg tggtgtggga gcggcaaaaa caagaaactg
    20701 gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt cttggtaatc
    20761 caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca acgctggcac
    20821 cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa aagcccgacg
    20881 cgcgcctgat tcgcctctct gaaaagaaca ataactggac gtttaatctt gccaacgatg
    20941 acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat aatattcttt
    21001 tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg gaaatttttg
    21061 ttgatccgtt aggcaagccg ctgccattca gcgaagttac tggatcgaaa ggtaaagcgg
    21121 ataaagaaaa ggtgggcgat tacatttttg gcctgaaggc gcagggacgt tataacggtg
    21181 aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc gaagggacgc
    21241 cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt gatggcgtcg
    21301 tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct ggcgattcac
    21361 tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg tttgaaacgg
    21421 atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat tatcgcggtt
    21481 ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc accggaaagc
    21541 cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg gacctgggac
    21601 cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct gaacagaaga
    21661 agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg
    21721 ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt gagcatggca
    21781 gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct gacctgcgcc
    21841 tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt catctggaag
    21901 gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg aaactgaaag
    21961 aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg
    22021 aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac ggcaacctga
    22081 aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt gggctgaatg
    22141 tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg aactgcgcgg
    22201 cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct ttcgatactg
    22261 agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcggaacag ctggatttga
    22321 ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg ctgtatgtgc
    22381 gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt gcccgtggtg
    22441 ctgttgctgc ggcactggca acgctggtaa caccggcggc ggcgttactg gcactgatct
    22501 caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg aagaagtgat
    22561 gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt ggcaaagtgt
    22621 gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc cttgtgaatt
    22681 caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt ttcacactta
    22741 ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg tcaagccagc
    22801 catcgccgcc agatataacc ccaccgcacc taacccgtag ttagcctgca accaggctgc
    22861 gatatacggc gcaacggaag ccccgagaat cgacgatacg ttgtaagaga acgatgctcc
    22921 ggtgtaacgc acttctgtcg gaaacagctc tggtaacagc gcacccatcg gcccgaaggt
    22981 cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg ggttgccaga
    23041 accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg tgatgatcac
    23101 catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca ccatcacgcc
    23161 aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca ggccaagccc
    23221 aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt aaaacagcgt
    23281 ataggttgcc agcataatga acgtacccag tacggttacg cgaacatgtt tggtcagcag
    23341 cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa acaccggcga
    23401 ctcatgcagc gacacgcgaa catacaggcc gataatgacc agcaccgccg agaagataaa
    23461 tggcacacgc cagccccagc tcataaactg ctcatcggtc agtagccagg aaagcagcaa
    23521 gaaagtgccg ttggcaaaga agaagccaat cggtgcgcct agctgtggga aggagccata
    23581 cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcac cgccccattc
    23641 accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagtg gggcgaaaat
    23701 accaatcgtg gcatagcccg gcagcagacc aatcaccacg gtcgaaatcc ccatcgttag
    23761 caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac caaaaacggc
    23821 agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact gtagcgttgc
    23881 tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg ccgcagtggc
    23941 gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga caaggacttt
    24001 attacgcgag ttgatcggcg tgtattcttg ttcgtggtcg agtgttgtgg ctgttgcttg
    24061 cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc aaaagtgata
    24121 tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg gataatgttt
    24181 aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag tggataaaac
    24241 tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta tgagttggag
    24301 tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt ttcaagaggt
    24361 tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta cggggagcca
    24421 ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct attttaaggc
    24481 tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat ccacgccgcg
    24541 caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag cacggaacca
    24601 aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat cacgatttta
    24661 atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt tttgcgcgga
    24721 cggtggcgcg gcaaccgcca gaagatccag aagaaaagca gatagttggc gaagatggaa
    24781 atcgccaggc caatcaatcg ccacgtcggt ttcagccact caatgctgtt caggtgcagg
    24841 gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag cgtcacaatc
    24901 agcgcaatca acaaaccaat cagtgaaata aaatcacgca gatattttac ccagaacttc
    24961 tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcacgaat cgcttcacgc
    25021 aggttaccca tccagttgat gccggaataa agtgccaccg ccaggccgac aagccctaca
    25081 gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc tagtgtcgga
    25141 tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg atgggaggcc
    25201 agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga caaaaatgag
    25261 aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg ttctgtcgcg
    25321 cgaataaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt ggtgacggtt
    25381 tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt aatcggctcg
    25441 tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg ctcttttgtc
    25501 cttctgtcgt ggtcattctt aaaaagtata gtcggtcagt ccaggtgctc tttcattacg
    25561 ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg ctgcgggtaa
    25621 accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat aagccgcccg
    25681 gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag accggcaaga
    25741 ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga cttaaaccac
    25801 tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag gtgcggtgta
    25861 taacgcacta ttgcgtgtga agtcagatca tcggggcttt gcggataacc aaagcgcgtc
    25921 agatagtgcg gactggcaca gttgaccatg gttagtttgc cgaggggacg ggcgataacg
    25981 ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg aagaatatct
    26041 accggacggt tatggctact cagttccagc tcaattcccg gatgctgata gagaaattcc
    26101 gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat gcgtaattta
    26161 ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag attactcaac
    26221 acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac ccgtcgcgta
    26281 gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg gcgcgaaaca
    26341 cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag ctccgcgact
    26401 ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg ttgttcacag
    26461 cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta atgccgggtt
    26521 tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg gaatagcttt
    26581 atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct ttaaagtgaa
    26641 tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt tattttggct
    26701 tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat aaggaaatcc
    26761 tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc tgtaactatt
    26821 tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc aaatggcgaa
    26881 ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta acgtattcag
    26941 gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt attcatggaa
    27001 taaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggtc agtcaggcag
    27061 acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc gatggcgatc
    27121 aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt cctgaggtta
    27181 aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt atccatttta
    27241 atgtgctggc cattgtgcct cgcgattcaa ccgtcgagac atttgcgttg gcggtaaata
    27301 gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg gaaaaggaca
    27361 ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg ttagcggcgg
    27421 gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg gtaaaagcgc
    27481 atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg gcaatgatgt
    27541 taaata
//
LOCUS       DAJVRP010000055        26628 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVRP010000055 DAJVRP010000000
VERSION     DAJVRP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.055"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(31..171)
                     /locus_tag="OQN12_002324"
     CDS             complement(31..171)
                     /locus_tag="OQN12_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCS1654624.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            413..706
                     /locus_tag="OQN12_002325"
     CDS             413..706
                     /locus_tag="OQN12_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1654625.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            778..1437
                     /locus_tag="OQN12_002326"
     CDS             778..1437
                     /locus_tag="OQN12_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1654626.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1529..1975
                     /locus_tag="OQN12_002327"
     CDS             1529..1975
                     /locus_tag="OQN12_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1654627.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2182..3082)
                     /gene="hlyE"
                     /locus_tag="OQN12_002328"
                     /pseudo
     CDS             complement(2182..3082)
                     /gene="hlyE"
                     /locus_tag="OQN12_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3455..3874
                     /locus_tag="OQN12_002329"
     CDS             3455..3874
                     /locus_tag="OQN12_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1654628.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3874..5142
                     /gene="umuC"
                     /locus_tag="OQN12_002330"
     CDS             3874..5142
                     /gene="umuC"
                     /locus_tag="OQN12_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCS1654629.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5188..5718)
                     /gene="dsbB"
                     /locus_tag="OQN12_002331"
     CDS             complement(5188..5718)
                     /gene="dsbB"
                     /locus_tag="OQN12_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1654630.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5864..7405)
                     /gene="nhaB"
                     /locus_tag="OQN12_002332"
     CDS             complement(5864..7405)
                     /gene="nhaB"
                     /locus_tag="OQN12_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1654631.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7626..8345
                     /gene="fadR"
                     /locus_tag="OQN12_002333"
     CDS             7626..8345
                     /gene="fadR"
                     /locus_tag="OQN12_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1654632.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8397..9929)
                     /locus_tag="OQN12_002334"
     CDS             complement(8397..9929)
                     /locus_tag="OQN12_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS1654633.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIIINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10259..11557
                     /gene="dadA"
                     /locus_tag="OQN12_002335"
     CDS             10259..11557
                     /gene="dadA"
                     /locus_tag="OQN12_002335"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1654634.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11567..12637
                     /gene="dadX"
                     /locus_tag="OQN12_002336"
     CDS             11567..12637
                     /gene="dadX"
                     /locus_tag="OQN12_002336"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCS1654635.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12805..14219)
                     /locus_tag="OQN12_002337"
                     /pseudo
     CDS             complement(12805..14219)
                     /locus_tag="OQN12_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14830..16566)
                     /locus_tag="OQN12_002338"
     CDS             complement(14830..16566)
                     /locus_tag="OQN12_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1654636.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16661..17575)
                     /gene="ldcA"
                     /locus_tag="OQN12_002339"
     CDS             complement(16661..17575)
                     /gene="ldcA"
                     /locus_tag="OQN12_002339"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1654637.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17675..18286
                     /gene="emtA"
                     /locus_tag="OQN12_002340"
     CDS             17675..18286
                     /gene="emtA"
                     /locus_tag="OQN12_002340"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1654638.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18288..19022)
                     /locus_tag="OQN12_002341"
     CDS             complement(18288..19022)
                     /locus_tag="OQN12_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS1654639.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19223..19477
                     /locus_tag="OQN12_002342"
     CDS             19223..19477
                     /locus_tag="OQN12_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1654640.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19527..20987)
                     /locus_tag="OQN12_002343"
     CDS             complement(19527..20987)
                     /locus_tag="OQN12_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1654641.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20924..21481)
                     /locus_tag="OQN12_002344"
     CDS             complement(20924..21481)
                     /locus_tag="OQN12_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCS1654642.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21507..22361)
                     /locus_tag="OQN12_002345"
     CDS             complement(21507..22361)
                     /locus_tag="OQN12_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCS1654643.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22361..23170)
                     /locus_tag="OQN12_002346"
     CDS             complement(22361..23170)
                     /locus_tag="OQN12_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCS1654644.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23180..23938)
                     /locus_tag="OQN12_002347"
     CDS             complement(23180..23938)
                     /locus_tag="OQN12_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1654645.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23935..24915)
                     /locus_tag="OQN12_002348"
     CDS             complement(23935..24915)
                     /locus_tag="OQN12_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCS1654646.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24915..25937)
                     /locus_tag="OQN12_002349"
     CDS             complement(24915..25937)
                     /locus_tag="OQN12_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCS1654647.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6701 a         6487 c         6865 g         6575 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcactatgg cctttgctga actcaacatt gacattctta cccgcagcaa agctcgtgga
      121 ggttagcagt gcaagcagca atacagcctt actgatgctc ttgattttca ttggtagtct
      181 cctgttcaga atgagtacga tgaactgtta taatataaca atcccttacg ggagaattcc
      241 agtggctttt ctgcgttcgg taaaatcccg gcgcggcatt ttttgccggg gggattactt
      301 ataaaggggc atggactcaa caaaccgcag aaatctacag ctggctggct tgtggcgcgg
      361 gtttcattga tgttagctta tgccaaaacc tggcatctta aagagtaaaa gtatgttttg
      421 tgtgatttat cgaagcagca agcgtgacca gacctattta tatgtcgaaa aaaaagacga
      481 tttttcgcgt gttcctgagg aactgatgaa aggttttggt cagcctcagt tagcgatgat
      541 tctgccgttg gatgggcgga agaaactggt caatgctgat attgaaaagg ttaaactggc
      601 attaaccgag caaggttact atttgcagtt accgccacca cccgaagatt tgctgaagca
      661 acatctttcc gtcatggggc agaaaacaga cgacactaac aaataaccga tatccggcgg
      721 tggcattatc tttgtcggcg cgggttttca tatccacgat aaggtgaggg gaacgttatg
      781 tatcaacatc acaactggca aggtgcgctg ctggattatc cggtgagtaa agtagtctgt
      841 gttggcagta actatgccaa acatattaaa gagatgggca gcgcagtgcc cgaagagcca
      901 gtgctgttta ttaaaccaga aacggcactg tgcgatctgc ggcagccatt ggcgatccca
      961 tccgatttcg gttcagttca tcatgaagtc gaactggcgg tgttgattgg cgcgacgctg
     1021 cgtcaggcaa cggaagagca tgtccgcaaa gccattgccg gttacggcgt ggcgcttgat
     1081 ctgacattgc gtgatgttca gggaaaaatg aagaaagccg ggcagccgtg ggaaaaggct
     1141 aaagcgtttg ataactcttg tccgctttcc gggtttatcc ccgcggcgga atttaccggc
     1201 gatccacaaa atacaacgct gggcctgagc gtaaacggcg aaaaacgcca gcaaggtacg
     1261 actgcggaca tgatccataa aatcgttccg ttgatcgctt atatgagcaa gttttttacc
     1321 ctcaaggccg gtgacgttgt gttgacaggc acgcctgatg gcgtcggccc gttgcaaagc
     1381 ggtgatgagc tgacagtcac tttcgatggg cattctttga caactcgcgt tttgtaatac
     1441 tttttgccgc cagaaagcgg cggcaaaact tgcatcgctg tgccagactg gttataaggt
     1501 gcgttttaac gtaatggcgg aacacctgat gagcgatgta cctttctggc aaagtaaaac
     1561 cctggacgaa atgagcgatg cggaatggga gtcgttgtgt gatggttgcg gtcagtgttg
     1621 cttgcataaa ctgatggatg aagacaccga cgaaatctac ttcactaacg tcgcctgtcg
     1681 ccagctcaat attaaaacct gtcaatgtcg gaactacgaa cgtcgtttcg agtttgaacc
     1741 cgactgcatt aaattaaccc gtgaaaatct gccaacattc gaatggctgc caatgacctg
     1801 cgcttatcgg ttgctggcgg aaggtaaaga tttacctgcg tggcatccgc tacttactgg
     1861 ttcgaaagcg gcaatgcatg gtgaacgtat ctctgtgcgt catatcgcag tgaaagaatc
     1921 agaagtgatt gactggcagg atcatatctt aaataaacct gactgggcgc agtgataaat
     1981 ttaatagatg ctgatggggg ctttcttaat atacgtctgg atgctcgcca tttcgtagcc
     2041 ctgtttttga aagcgctctg tcataatgag agttacccga taccttatta tcaacaggta
     2101 actctcaatc tttaaaaaac gtctgtgttt ttaaatgtga taaaccttat accttgagta
     2161 catggagaga ataatcgctt atcagacttc aggtacttca aagagtgtct ttttaccgtg
     2221 tcttttctga tactcattac aggtgttaat catttttttg gccgctgctt ttagcaaaga
     2281 aagcattaaa tcatcataat caacatagaa tctggttgtt tcagtttccg tttttatctc
     2341 cccgatggcg gctatttcgg tggttaattt caatttggcg gcatcgatat ctttattcgc
     2401 ttgtttaacc gtgttagaca gggtggtaaa gaaactctgc acagatttta acttgttctt
     2461 caattctgga atcagtttcc cttcaactac gcccgcagca atagaatagg aaatgattaa
     2521 accaaatgga ccggcgacga catcggctgc ggcaccggca tacgcttcct tcctgatttt
     2581 atctacctgt gactggaaat agctgctttt ttctgaaaaa tcattggtta actggctatc
     2641 taacgccagc agtttcccgg aagcgttgtt gaaactttgt gagcttacca gcagggattt
     2701 ttgcgcttca ttcagcttcg tgatgccgtc atccagtacc ttaatgaggg gcggatgctt
     2761 tcttctcatt gtactcatca aatagcaaaa tatacgctgc gagcaattgc gtcgcaacac
     2821 cacaccattc atacactgtt tgggttgctt caaaatactt atcctggcta tccataagta
     2881 aggttttaat atcgccgact aaaacggagg ctgcctgtga atactcctgt ttaaagcgac
     2941 ttaactcttt tatggtttca tcaaaggtct gccaggggat gacctgatcg agatatttat
     3001 tataaagatc taatgctcca tctgcggttt cgattgcgtt tttaactact tctaccgttt
     3061 tatctgcaac gatttcagtc ataatcattc gcctctttaa atatataaat tgtaatgaaa
     3121 ctcctgtttt acaactatta ataaatttta cttcatctaa ttcatagtta gccgggcggg
     3181 atgcgtcaat gtctttattt ctattaatat gataaatatc aaacaatgtt taatgtcatt
     3241 atggcgaatg cttctattct atttttttgc cgggtgatat ttttcatttc tgctggatga
     3301 gcgtcgtcgc cagaaggcca cgtgagcaca ggataagaga acgaaaaatc agcagtctat
     3361 gcagcgacaa atattgatag cctgaatcag tattgatctg ctggcaagaa cagaccactg
     3421 tatataaaaa cagtataact tcaggcagat tattatgttg tttatcaagc ctgcggatct
     3481 ccgcgaaatt gtgacttttc cgctatttag cgatcttgtt cagtgtggct ttccttcacc
     3541 ggcagcagat tacgttgaac agcgcatcga tctgaatcaa ctgttgatcc agcatcccag
     3601 cgcgacttac ttcgtcaaag caagtggtga ttctatgatt gatggtggaa ttagtgacgg
     3661 tgatttactg attgtcgata gcgctattac cgccagccat ggtgatattg tcatcgctgc
     3721 tgttgacggc gagtttacgg tgaaaaaatt gcaactacgc ccgacggtac agcttattcc
     3781 catgaacagt gcgtactcgc ccattaccat cagtagcgaa gatacgctgg atgtctttgg
     3841 tgtggtgatc cacgtcgtta aggcgatgcg ctgatgtttg ccctctgtga tgtaaacgcg
     3901 ttttatgcca gctgtgagac ggtgtttcgc cctgatttat ggggtaaacc ggtggttgtg
     3961 ctatcgaata atgacggttg cgttatcgcc cgaaacgctg aggcaaaggc gctgggcgtt
     4021 aaaatgggcg atccctggtt caaacaaaaa gatctgtttc gtcgctgtgg tgtggtttgc
     4081 tttagcagca attatgagct ttacgcagac atgagcaatc gggtgatgtc gacgctggaa
     4141 gaactatcgc cccgcgtcga gatttacagt attgatgaag cattctgcga tctgacaggt
     4201 gtgcgtaatt gtcgcgatct gactgatttt ggcagagaaa ttcgcgcaac ggtgctacaa
     4261 cgtacccatc ttactgttgg tgtggggatc gcccagacca aaacgttggc taagcttgcc
     4321 aatcatgcgg caaaaaaatg gcagcggcag acgggtgggg tggtggattt atcaaatctg
     4381 gaacgccagc gtaaattaat gtctgctctc cccgtggatg acgtctgggg gattggacgg
     4441 cggatcagca aaaaactgga cgcgatgggg atcaaaaccg ttctcgattt ggcggataca
     4501 gatatccggt ttatccgtaa acattttaat gtcatgctcg aaagaacggt gcgtgaactg
     4561 cgcggcgaac cctgtttgca actggaagag tttgcaccga cgaagcagga aattatctgt
     4621 tcccgctcgt ttggtgaacg catcacggat tatacgtcga tgcggcaggc catttgtagt
     4681 tacgctgccc gggcggcgga aaaacttcgc agtgagcatc aatattgtcg gtttatctcc
     4741 acatttatta agacgtcacc atttgcgctc aatgaacctt attacggcaa tagcgcgtcg
     4801 gtaaaactgc tgacgcccac tcaggacagc agggatatca ttaacgctgc tacgcgatct
     4861 ctggatgcca tctggcaagc gggccatcgc taccaaaaag cgggcgtgat gctgggggat
     4921 ttcttcagtc agggagtcgc gcagctcaat ttattcgatg acaacgcacc gcgccccggg
     4981 agtgagcaat tgatggcggt aatggatact ctgaatgcta aagagggcag aggaacactc
     5041 tattttgccg ggcaggggat ccaacaacaa tggcagatga agcgagctat gctttcacca
     5101 cgttatacaa cgcgaagttc tgatttactg cgggtcaaat aaatatagcg gcaggaaaaa
     5161 agcgctcccg caggagcgcc gaatggatta gcgaccgaac agatcacgtt ttttcgcttt
     5221 aaacggctgg gaaatcacca ccagcaccgc gacaatcagg taagcgataa aaataccgag
     5281 cagccactgc ggcatttcca tacctaaaaa atcccactga cgctcggcgc aatcgccaga
     5341 ggcgacaaac acttgcggca cccatttatc cagcggcagc cattccggga aacgaaccat
     5401 aaaatcacag gtggcaaatg gcgaaggata gagctgaagc atggtgtgct cgtaagttaa
     5461 ctgcacaccg cggaacgcac tatacaacca gataaccatc gctacataac gcagcggagt
     5521 tttcggggcg atcgcgccaa tcagcgcagc acccagaacg ccgaataacg cgcagcgttc
     5581 ataaatacag agcacgcaag gtttcagtaa catcacatgc tggaaccaca gcgccgtcag
     5641 ttccagtgcc agagcagtaa acgccatcaa cagccacgcg ccccggcctt gtgaacattg
     5701 gttcaaaaat cgcaacataa tcatttccct gcaatatgca tagagtgcgc agtttaaacc
     5761 aattcattcg ctgcgccacc aggaggcagc aaaaatagga cgcaatagga caattatcgg
     5821 gcgaaaaggc aaaccgggca tttatatgcc cggtaagttg ttatcaaagc gttgctatcc
     5881 agcccatttg cataaaccat tcggttacag gggcaagcgt aaactcgacg cagagcaagc
     5941 cgacgagtgt cagtacgagg gtgtaaggca gggccatcca caccatgcgg ccataagaga
     6001 ggcgaatcaa tggcgcgagt gcagaggtca gcaggaacag gaacgcagcc tgaccgttcg
     6061 gcgtagcgac agagggcaga ttggtaccgg tattaatggc gaccgccagc agctcgtatt
     6121 gcttcaacgt gatagcgcca ctttccattg ccgctttcgc ttcgttgata taaatcgtcc
     6181 ccacgaagac gttatccgaa atggatgaca gcagaccgtt gaaaatatag aacagcgaca
     6241 gctgagcatg ttcagatgcc tgcaacacaa actgaataat cggcgaaaac agttgttggt
     6301 cgataatcac cgcgactacc gagaaaaaca ccgtcaacag tgcggtgaat ggcagagatt
     6361 cggtgaaggc tttaccgata gaatgttcat cggtgacacc ggtcaatgat gttgccagaa
     6421 taatgactga caaaccaatc aagccaactt ccgccagatg caacgccagc gcagtcacca
     6481 gccagacgcc aataatcgcc tggacaatca gacggatttt atcctgacgg gtacgctggt
     6541 ggcggctttg atcgtcaaac tgttgcaaca cttcgcggac tttctccggc agcgtttcac
     6601 cgtagccaaa ccaacgcagc ttctctacca gcaggcaggt taacaggcca caaatcagaa
     6661 ccggaacggt caccggcgac atgcgcagga agaaatcgcc aaaatgccag ccagccgctt
     6721 tagcgatgat caggttctgt ggttcgccca ccatggtcat tacgccgcct aatgcggtgc
     6781 cgacaccggc atgcatcatc aggctacgca gaaagccacg gaactgttcc agaaccacgt
     6841 tgtaatgctt gtcgatatga ctatcgtctt gcaggtcggt gtcttcggta cgggaagagg
     6901 ctacgcgatg ataaatacca taaaaaccga ctgcaacgct gatcaccacc gccacgacgg
     6961 ttaaggcatc gaggaacgcg gagaggaacg ctgccgccac gcaaaaagag agcgacagca
     7021 gcattttgga gcgaatgctt aacagcaaac gggtaaatat gaacagcaac agctgtttca
     7081 taaaatagat acccgccacc ataaacatca ataacagcaa gacttcaaga tttgccgcca
     7141 cctcttcacg gacgtgttcc gcgctggtca tgccgatgaa taccgcttcg atagccaaca
     7201 gaccaccggg gagtagcggg tagcatttca gggccatcgc cagagtgaaa ataaattccg
     7261 ctaccagcaa ccagcccgcg acgaaagggc tgatgaggaa aattaacggg tttacgatta
     7321 agaaaatgat gagggcgagt ttgtaccagt cgggggactg gcccaaaaaa ttgcgccata
     7381 gcgcgcggcc ccaggagatc tccatgatgg tttcccttac cttacaaata atcaatgatg
     7441 tttttatgtt taaacgcaaa gcttaacggt caggcaggag tgaggcaagt cttgatagtc
     7501 aaggggaaag agatgcggaa aatgaagcct tgatcccttt ttcttctttt tgtctgctat
     7561 cagcgtagtt agccctctgg tatgatgagt ccaactttgt tttgctgtgt tatggaaatc
     7621 tcactatggt cattaaggcg caaagcccgg cgggtttcgc ggaagagtac attattgaaa
     7681 gtatctggaa taaccgcttc cctcccggga ctattttgcc cgcagaacgt gaactttcag
     7741 aattaattgg cgtaacgcgt actacgttac gtgaagtgtt acagcgtctg gcacgagatg
     7801 gctggttgac cattcaacat ggcaagccga cgaaggtgaa taatttctgg gaaacttccg
     7861 gtttaaatat ccttgaaaca ctggcgcgac tggatcacga aagtgtgccg cagcttattg
     7921 ataatttgct gtcggtgcgt accaatattt ccactatttt tattcgcacc gcgtttcgtc
     7981 agcatcccga taaagcgcag gaagtgctgg ctaccgctaa tgaagtggcc gatcacgccg
     8041 atgcctttgc cgagctggat tacaacatat tccgcggcct ggcgtttgct tccggcaacc
     8101 cgatttacgg tctgatcctt aacgggatga aagggctgta tacgcgcatt ggtcgtcact
     8161 atttcgccaa tccggaagcg cgcagtctgg cgctgggctt ctaccacaaa ctgtcggcgt
     8221 tgtgcagtga aggcgcgcac gatcaggtgt acgaaacagt gcgtcgctat gggcatgaga
     8281 gtggcgagat ttggcaccgg atgcagaaaa atctgccggg tgatttagcc attcaggggc
     8341 gataatccct tccgtttaaa gagcaacccc ctcaaacgag gggttttttg ttgtttttac
     8401 agatttccca ttcttggcgg gcaacgttcc agcaactcga tgctgccgtc ttcgttttgc
     8461 tgttcgagca tcacatcaaa tccccacagg cgatgcacat gcttcaggac ttctttgcgc
     8521 ccccgatcca gcggtgcgcg attatgtgga atataacgca gcgtcagcga acggtcgccg
     8581 cgcaaatcca cgttccagat ctgaatattc ggctccagat tacttaagtt atattgcgac
     8641 gataaccggt tacggatctc ccgataacct tcttcattat gaatagcgga aatctccaga
     8701 taattatgcc gatcatcatc cagcacggtg aagaagcgga aatcacgcat cactttcggt
     8761 gacagaaact ggctgataaa actctcatct ttgaaatcac gcatcgcgaa atgcagcgtt
     8821 tccagccagt cggaaccggc gatatccggg aaccagtatt tgtcttcttc cgttggcgac
     8881 tgacaaatcc gtttaatatc ctggaacatg gcgaacccga gggcatacgg gttgatgccg
     8941 ctgtaccacg ggctgttata ggggggctgg aagaccacat tggtgtggct gtgcaaaaac
     9001 tccagcataa aacgttccgt tactttccct tcatcataca gatggttaag gatggtatag
     9061 tgccagaagg tcgcacagcc ttcgttcatc acctgagtct gtttttgcgg ataaaaatac
     9121 tggctcacct tacgcacaat acgcaggatt tcacgctgcc atgattccag cagtggcgca
     9181 tttttctcca taaaatagag caggttttct tgtggttcgg acggatagcg gcgcgcttca
     9241 gcaaccgttt tctcttcctc gcgcttcggc agggtacgcc agagcatatt gacctgactt
     9301 tgtagatact cttcgcgact tttctgccgg gctttctctt cttgcagcga gattttttgc
     9361 gggcgtttgt accggtccac gccgtagttc atcagcgcgt ggcacgagtc cagaagccgt
     9421 tctacttcat caacgccata acgttcttcg cactcggtaa tatatttacg ggcaaaaatc
     9481 agataatcga caatcgaact ggcgtcggtc caactgcgga ataagcaatt gtttttaaag
     9541 aaagagttat gtccatagca ggcgtgtgcc atcaccagcg cctgcatagt aatggtgttc
     9601 tcttccatca agtaagcgat acacgggtta gagttaatga tgatttcata ggccagccct
     9661 tgctgaccgt gcttatacag ccgttcagtc tcgataaact ttttaccgaa tgaccagtgc
     9721 ggatagttaa ttggcatgcc gacgctggaa taggcatcca tcatctgttc tgaggttatc
     9781 acttcaatct ggtgcgggta ggtatccagc cggtagagtt tcgccacccg gtctatctct
     9841 gccagataaa catccagcag gtcgaacgtc cagtcgggtc catcgctcaa acgtgtggtg
     9901 tccttattca tagaatcgat cgtcgccata cacgcacctc attgttgtcg gcgctctctg
     9961 tgtggagcac ctcatttcaa gcatagaaca cctgttaaaa accgcgtcgc cggagaattt
    10021 ttttctttgc gatttcttat tatcagagtg ccactaatcc gcttctgaac ggaattttat
    10081 gctggataaa aagggcgttc agcaggagat actaaagacg ccatattgcc gcagagtcag
    10141 ggagatgtga gtcagctcac cataaaaaag ccgtatgttg aataatattt tcaactgagt
    10201 tatcaagatg taattagatt attattcttt tactgtatct accgttatcg gagtggctat
    10261 gcgagttgtc atactgggaa gtggtgtggt aggcgttgcc agcgcctggt acttaaatca
    10321 ggcaggacat gaggtcaccg tcattgatcg ggagccgggg gccgccctgg aaaccagtgc
    10381 cgcgaatgcc gggcaaatct cccccggata tgctgcaccg tgggcagcac caggtgtgcc
    10441 tttaaaagcg attaaatgga tgttccagcg ccatgcgccg ctggcggttc gtctcgacgg
    10501 tacgcagttt cagttgaaat ggatgtggca aatgttacgt aactgcgaca ccagccacta
    10561 catggaaaac aaagggcgga tggtgcgtct ggcggaatac agccgtgatt gcctgaaagc
    10621 attgcgcgcc gaaaccaata ttcagtatga agggcgtcag ggtgggacgc tgcaactgtt
    10681 ccgtaccgaa caacagtatg aaaatgcgac ccgcgatatc gccgtgctgg aagatgccgg
    10741 cgtaccgtat cagctgctgg aatccagccg cctggcggaa gtggatcccg cgctggcaga
    10801 agtagcgcac aaactgacgg gcggcctgca gttacccaat gatgaaaccg gagactgcca
    10861 gctgtttacc cagaatctgg cgcggatggc agagcaggcg ggggttaaat tccgctttaa
    10921 tacgcccgtt gaccaactgc tttgcgacgg cgagcaaatc tacggcgtga agtttggcga
    10981 tgaagtgatt aaggccgatg cgtatgtgat ggcgtttggt tcttactcga cggcgatgct
    11041 caaaggcatt gttgatattc cggtgtatcc gctgaaaggc tactcgctga ctattccaat
    11101 tgcgcaggaa gatggtgcgc cagtatccac cattcttgat gaaacctaca aaatcgccat
    11161 tacccgtttc gataaccgca ttcgtgttgg cggaatggcg gaaattgttg gttttaacac
    11221 cgagctgttg caaccgcgtc gtgaaacgct ggaaatggtg gttcgcgatc tctatccacg
    11281 cggcggtcat gtcgagcagg cgactttctg gacgggtttg cgcccgatga cgccagacgg
    11341 tacgccggtt gtcgggagta cacgctttaa aaatctgtgg ctgaataccg gtcacggcac
    11401 gctcggctgg acgatggctt gcggttccgg tcagttgtta agcgatctgc tctctggtcg
    11461 cacgccagcg atcccatatg aggatctaag cgtagcgcgc tacagccgtg gatttacgcc
    11521 atcacgtccg ggccatttac atggcgcaca cagctaagga aacgagatga cccgtccgat
    11581 acaggccagc ctcgatctgc aggcattaaa acagaatctg tccattgtcc gccaggccgc
    11641 gccgcacgcg cgcgtctggt cggtggtaaa agcgaacgct tacgggcatg gtattgagcg
    11701 tatctggagc gcactcgggg ccaccgatgg ctttgcatta cttaacctgg aagaggcaat
    11761 aacgttacgt gagcgcggct ggaaggggcc gatcctgatg ctggaaggat ttttccatgc
    11821 tcaggatctg gagatttatg accagcaccg cctgaccacc tgcgtacaca gcaactggca
    11881 gctcaaagca ctgcaaaatg cgcggctaaa agcaccgttg gatatttatc ttaaagtgaa
    11941 cagtgggatg aatcggttag gcttccagtc cgatcgcgtg cttaccgtct ggcagcagtt
    12001 gcgggcaatg gcgaatgttg gcgaaatgac cctgatgtcg cattttgctg aagcggaaca
    12061 tcctgatgga atttccggcg cgatggcgcg tattgagcag gcggcggagg ggctggagtg
    12121 tcggcgttcg ttgtccaatt cggcggcgac tctgtggcac ccggaagcgc attttgactg
    12181 ggttcggcct ggcattattt tgtatggcgc ttcgccgtcc ggtcagtggc gtgatatcgc
    12241 caataccgga ttacgtccgg tgatgacgct aagcagtgag attattggtg tccagacgct
    12301 aaaagcgggc gagcgtgtgg gctacggcgg tcgctatact gcgcgcgatg aacagcgaat
    12361 cggcattgtc gccgcaggat acgccgacgg ttatccgcgc catgcgccta ccggtactcc
    12421 tgttttagtg gacggcgtgc gcaccatgac ggtggggacc gtctcgatgg atatgctggc
    12481 ggttgattta acgccttgcc cgcaggcggg gattggtacg ccggttgagc tgtggggcaa
    12541 ggagatcaaa attgatgatg tcgccgccgc tgccggaacg gtgggctatg agttgatgtg
    12601 cgcgctggcg ttacgcgtcc cggttgtgac agtgtaactt gttgtaagcc ggatcggagg
    12661 cagcgtcttc tggttgcgaa aaaatcatcc atccggctgg tcagcaactg tagttgttaa
    12721 tgtgacagag ccattgccca tgatagtgtc cattaaaaga atggacacta tttccccgga
    12781 acctgaactc gccgcacagg cgttctacat aaaacgctta cgcttcattg ttgactcgga
    12841 gtcgacttca gataaatcac gcttcaccct tgatgcagat tcgggttcat gttcagattg
    12901 ttgattaaca ggctgcgagt cctgttgtct ttccagactc aacaggccca actgttgatc
    12961 caggagctcc aggtcctcct gtaccttcac gcgctccagg agttccgggt catccaggaa
    13021 ctccagatcc ataaggtcct ccagctcctc gagtccttcc aggtcaacct agccttcctt
    13081 gtgcaaaaag tccaaaaggt ccttcaggcc ctgctgtttt ttcaggccct cgacggcttt
    13141 gagcagcgtc ggtaaattgt ctttgtccca cgggttacca ttacagtcaa acaccttcag
    13201 gctctggggc tgcggcacct gtaccgtttt aaacttgttt tctccacagt agtaaattat
    13261 tagtttgggc ggcagccgtg gtgaagagat caaatcgtta ctagaacagt caaatacttt
    13321 caggcgagtc aagttctcca gcatctcatc aggtattgag ttaatattgt tgccggcaca
    13381 ggatagttta tacaggtact ggggcatcga cggcggtaaa aattgcaaga agttttcaga
    13441 acagttaatc actctcaggt tagtcgacag ctcaggcagc cagataatac tgttttcata
    13501 gcacttcagt accatcagtt ttgagggctg caccttcggt aacaagatca agacgttatc
    13561 tgtacagtca agcaccagca gccactttgg catcaccttc ggtaacgcgg tcaacacgtt
    13621 tttactgcag taaaattcaa tcagatgtgg aggcaaattc tcagggagtg aagtcaatcc
    13681 caggttgcta agattgagca tactccgtct tgtaatgaga cattccttca ttctgcgcaa
    13741 tgcttctgcc cggttttcaa tccggcttgc tggcaggttt tcttccagga tattttcctt
    13801 gatttcttcc agactttctt tcaggtcttc ttggggaatt tctgactgtt tgttttgctg
    13861 gttttcttcc aggatttcgt acaggttttt ttgcaggttt tcttgcaaac catcctgctg
    13921 tatagcccat tcgcaccatt tggcgaccat catgaactcg ttatgttgct gattcagacg
    13981 agtccccaac gtgatgatgg tattgttgtc cacctgtatg ttcactttgc ccgcgatact
    14041 gaggtcaatg atctgccagg gggcatcacc ccagctgaat ttttgcgggc tgagatactt
    14101 catgttctgc aaaatttgat tcttgagaag agccgccctg gcgggcgtcg tacgcctggg
    14161 ttaacggaag atgggggaat ggcgggggcg aatgggcaga tgtctttaaa gctagccata
    14221 atgctacgtt tccttgaaat aaatatcctc tggcagcgct ggagacgtga atgtggtcac
    14281 cgcaaaggag ccacaggacg tcatttgcgc tgatatcact actcatcgtt gactttttcc
    14341 ctggtcagaa atgccgtgtc gggtctggta catgctgtat tacggcctag gtagctctga
    14401 cgtattaaag aaaatacgag agtgaacgac ccggaaataa tcgttattaa gcaaaggaat
    14461 aactataaga aatcgctcat gcatccggtg ctacaggcgg atgaaacgcc tattgtgtgg
    14521 gctatgtcag tgcggcaggt tgcgtggggg aatatgttca ggcgatgtca ggcaggagcc
    14581 cctgatggtt gaccggtaat gcggggctgg tttttattac ctgttacagt cggaaaaagc
    14641 ccttacgctg actttcttga gccggtgttt tgagttcatt agccagagcc tgcaatggtt
    14701 tgtcatccat aatttaacct tcttctgatg ttgggatgag catatcaaaa tcaggaaatg
    14761 acaagtttat tacaggctca ggctgcgttc agtaaagcat cgcatccggc gatagatgct
    14821 gcattaaatt taagattcag cttcctcttc agccacccga acgccaatct tcaacacttc
    14881 attatcttct ttctcggcca ccgtccagat catcccggca aactctacct ggtcaccgac
    14941 aaccggtgcc gcgcctagca actgctggac aatttcaccc agcgtttgct gtttatcacg
    15001 atattctcgc ccgtcttcca ggccatatat cagcgcaaca tcagcatatt tggcgctggc
    15061 ttcgagaatg aagtcaccaa agaagcgttg atccagcgcg accggtggcg actggctgaa
    15121 cagtttaccg agcgccggga gatcgcgttc ccgaccaatt acacacaata catcgccttc
    15181 gcgcagtcgg gtgctgccgg taggatgaag caactggtta tcacgaaaca gggctgcaat
    15241 acgcgtctct tttggcatat gcaaatcgcg cagtgccgca cctacacacc atttatcagc
    15301 actcaattga taaacaaact gctcccacgg attttccgga tgaatatcca ggccaacgcg
    15361 tgacaccgga cgtccgacgg gcggaaccac cactttggct tttttcgccg cccacgagag
    15421 tgatgttccc tgcaagagca gtgaaaccag aaccacaaag aaggcgacat taaagaacag
    15481 tcgcgcattc tccagccccg ccatcatcgg gaacacggct aggatgattg gcaccgcgcc
    15541 gcgtaatccc acccagctga taaacacgcg ctcacgcaga ttgaaaccgc ggaaagggag
    15601 caatccagca aataccgaaa ggggacgggc gaagaatatc atccatgccg acaaaatgag
    15661 cgccggaatg gcaataggca gcagatcgct tgggttaacc aatagcccca gcaccaggaa
    15721 catggcgatt tgcgccagcc aggcgaggcc gtcgaaattt tgcaggatgc cgtagcggtt
    15781 gcgaatcggc cgattaccca gcagaaaacc gcacagataa acagccagaa taccgctgcc
    15841 ttccagcgcc gtggttaaag caaaaattaa aataccgccg cttaatgcca gcaatggata
    15901 taatccggca ggcagggcga tgcgattaat catttgcagc agtaaataac caccgccaag
    15961 accgatgaca atcccgaggc caaattgctg cagaatatcg accacgaaca tccaactgac
    16021 actgctttca tgctgctgga tcattgcaat cagggtaatc gtcagaaaga ccgccattgg
    16081 atcattactg ccggattcaa tttccagcgt cgagccaaca cgttcgttaa gccccttacc
    16141 acccagcaga gaaaagaccg ctgcagcatc ggtggagccg acgatagcgc cgattaataa
    16201 gccttcaatc aaatcaagat taaacagcca cgccgccatc atgccggtta aaccagaggt
    16261 gataagtacg cccagcgtcg ccagcgacag cgccggtcct aacgccacac gaaaggaact
    16321 ggcctgagtg cgcatcccgc cgtcgagcaa aataatcgcc agcgccaggt tacttaccat
    16381 gtaggcgaag gggtaattat caaacgggat accgccgacg ccatcgactc ccgccagcat
    16441 gccgatcgcc agaaaaataa ccagaatagg aatgccaaga cgggaagaaa atgaactaag
    16501 taatatactg ctggtgacaa ggatggatcc taagatgaaa aggctaatta ttgttgtggc
    16561 atccaacggt cggttactcc tgattacgct gtctcttata taaaccctac catattagcg
    16621 gcagagacag cgttttactt agccctgaag cgtgattttt ttacatttta agaacaggat
    16681 gaccgctaat cgtcaactga gcgccttccc gggtgttatt cagaatggca tgtgcgccca
    16741 acggcagcgt aaccgtgcgt tgttcatgac caaaatcgag gccggtaatg agtggaatcg
    16801 acaggcggga acgcaaaaac gcgtacactg actccaggtt gtaacctgcg tcataatcat
    16861 tgggcgtgct gccgctaaag ctaccgagaa taatcgcctt ctgacgtggc aaaattcccg
    16921 catgatagag ctgcaacagc atacgttcga cgcggaaagg gtgcacgtta atatcttcca
    16981 gcaccagaat accgttctca atttttggca tccacggtgt accaatcagt gaaatcagca
    17041 tcgcaagatt gcctccccac aacgtgcctt cggtctgaca tgtcgggcct tccccttgcc
    17101 attcaatggt gaaggtttca ttgcgtaacg ccagccagaa atggtgctcg gtaaaggcgt
    17161 tcagttcatc cgcaccaaag tttgctacca gcatggggcc gctaaaggtg atgacattgc
    17221 cttgcgccag aagaccgcac tgaatggcgg taaaatcgct atgtccgcaa atgagtaacg
    17281 ggtcatgttg ttggcgagcc accagtgcct gccagtcaat atctgccagt aaacgactgg
    17341 caccgtaacc gccgcgtaca gccagcacga tggtgttggg ggtagtcagt ctggcaaggg
    17401 aattgagatc ttccagacgc tccgtttccg tacccgcaaa acgctcacaa cgacgggcaa
    17461 taacctcgac gttattgact tgatgccccg cgtcggtaag gcgctggata ccacgcagcg
    17521 cggcgtgctg tttaatgctg taacccgatg gggcaattaa gtgaaacaga gacatggcaa
    17581 ttccttgctg acaacagaaa cgaaatgtat atcatgccgc ttaggtgtgc cgttgtcacc
    17641 tcaacggcga ttccaggcta taaggataga agaagtgaaa ttgagatggt ttgccttttt
    17701 gattgtgtta ttagcgggtt gttcatcaaa gcatgactat acgaacccgc cgtggaacgc
    17761 gaaagttccg gtgcaacgtg cgatgcagtg gatgccaata agccagaaag ccggtgcagc
    17821 ctggggcgtc gatccacaat tgatcacggc gattatcgct atcgaatcgg gtggtaatcc
    17881 gaacgcggtg agtaaatcga atgccattgg tttgatgcag ttaaaagctt caacctccgg
    17941 acgtgatgtt tatcgccgta tgggctggag tggtgagccg acgaccagcg agctgaaaaa
    18001 tccagagcgt aatatttcaa tgggggcggc ttacctgaat attctggaaa ccggcccgct
    18061 ggcaggcatt gaagatccga aggtactgca atatgcgctg gtggtgtcat acgctaacgg
    18121 ggcaggtgcg ctgctacgga ctttctcgtc agatcggaaa aaggcgatca gcaaaatcaa
    18181 cgatttggat gctgacgagt tcctcgatca cgtagcgcga aatcatcctg cgccgcaggc
    18241 tccgcgctat atctacaaac ttgagcaggc actggacgcg atgtaaatca gtcgcgcact
    18301 ttgtccgctt tttcccgggc ttctcgctcg agagagaaaa taatccgctg taattgccgc
    18361 tccaccgtcg ggctgacgtt aagaaaacgg aagctcagac ggggagtggt gatggtttca
    18421 ttcttgccat caatcacttt gcgctcgctg gtggagatta actgggcgtc aaagtgaaaa
    18481 acaccccatt gccccatgtt gacttcaatt tgagcgaagc gcataccttc ttgtaattcg
    18541 gcaggctttg ctgtttccag taatgcgccc atgccgccta acgacaaatc atacaagcgg
    18601 aaacgtaacg tactgttatc cgccagtttg gtctggcaaa aataaggcgg atggagtggg
    18661 gcggagatgc ggaaatatcg gcgtcgttgt acaaaccata aggtgggagg cggtacggta
    18721 ataaatgccg gaagctgcaa gtattcactc tgctgtagtt gttcaacagt aaactcgact
    18781 ttcgcaccct gagtttcggc ggtaatggta atgtgctgtg cctttagcac ggcgatgttg
    18841 tcttcggctt gactgccgaa atccagcacc agtttatccg gggttattgc cagtattttg
    18901 ctgatcagct gcccgccatt ccaactgaga cgcaaaggaa ttgcggcttt gtgcaaatcg
    18961 cgtaacacgc ccaggacggc taacggattt tgtttcagga actgctcatg gtaatgactc
    19021 acgcggaaaa actcctgact gacaaactgt ctttggttta tcggtcatag ttaacaaaac
    19081 ttaatacaaa tgcgtgaata ttttttacat gttgttctta aatcagccga caaacgccca
    19141 gcatcgatcc cgcccttgcc tatacttaga gcgttgacgt aagcatttct tgcgaccgat
    19201 tcatcgaaac gagggcctga acatgggaat tattgcctgg attatttttg gcctgatagc
    19261 cggcattatc ggcaagctaa tcatgccggg gcgtgatggt ggtggatttt tcctgacctg
    19321 tattctcggg atagtcggtg cggtggtcgg cggctggctg gcgaccatgt ttggcattga
    19381 cggctccatc agcggtttta atttgcacag cttcctggtg gcggtggtgg gcgctatcct
    19441 ggtgctgggc gtattccgcc tcctgcgaag agaataagat tttcataagg cggatagcga
    19501 tacagatgcc gctatccgct ttcacatcag aacgtatact cgacacctgc ccagaaattg
    19561 cgaccttctt caataaaccc ttcactgtag gcatattggg tatcaaacag gttattgacc
    19621 gacgcattga cgctgaagcc atgacctaag gtgtaatcgg ctcgaatgtg ggtcaccgca
    19681 aaaccggcgg ctttttgtga accgtcactg ttgctgtagc tggaggaacg cgcctcttcc
    19741 gacagcgtta cgcttaacgg ctcccatggt ttgagtgtca tccatgcggt cattgtctgc
    19801 gttggaagat cggttatctt gccgatgtct ttgcgtttgg cgtcagcgtg gatcagagcg
    19861 tagctcaatc ctacatccag tatattgctg attttcccct taataccggc atccagaccg
    19921 ctgtaatcca ccgtgccgct gttctgattt tgaatggtat cggcattgat attgtgcgag
    19981 aggatggcat cactcacccg gttatagtaa acgctgacct caaatcccca gtcgcgcgtg
    20041 aaggcaccat tccaggttaa atccaccccg cgagcgcgtt ccggcttgag ctgcgggtta
    20101 acaatcgcta tctggttata cgcaggtttg gatgtggtat agcgttcttt cagcgtcgga
    20161 aagcgtgtgc ggtcatagta cgaaagcgcc agagtgtctt cattggcaaa gtggtatttc
    20221 cccatcacct gccagttaaa agctgactga ttgttgtcgt cataatgggt gatgctgcca
    20281 tctttctcgt gttttttcgc ttctacgcta tcgcgccagt catagctgat tcccgccacg
    20341 acatcgacat tatcagcagc agcccattga tattcactgg cgagcgacca ggtacgatct
    20401 tcatagcgat cgtaagcggc gtgcggcgca cctttttccc ggtgtacgtc atctttccag
    20461 ttaacggcaa acgacagcag atcgttttca cgcacatcgg ctgccagttg taatccggca
    20521 ccgtcgctgt aatcggaata atggctgtag ctgccttttt tatttttcaa atcagccagc
    20581 gagttgtaca tcatcagcgt attttcaaag gtgtcgcgat acagccgact tttcagggta
    20641 aaacggtcgt ttagttgggt cgctccctga taataaaaac tttctttgtc atactctggc
    20701 cactgccagt agcgtgattt ttgaccacaa tttccgctgt atggcgggtt atctttttca
    20761 ccatcctgct taatgtaagt cagcgtgtat tcatcgtttt cgcgcggtgt aaaaccgagc
    20821 ttcacaatgc cgcgtttatc atcagccgat gaattaatca tcttgccgtg tttgcctgca
    20881 atatcattat ttacaccatg cggcaggccg agaaaatcct gctttagctg gctaccgctg
    20941 acttgcaaat acccaagatc gctgctggcg gcaaatgaag catgcatatc gtaggcattg
    21001 tcctgactac ggctccatcc ctggcgatat cccagacttg cttcttcagg gtggtgatat
    21061 taatggctcc gcccatctga ttaggcccat gaagcagcga cgaataccct ttggaaactt
    21121 cgactgcccc cagattgttg gtcagaatcc gcgccagatc gaggttgccg tcatagggga
    21181 cataaatggg cacaccgtcg aaatagaccg gcacctgccg actatcaaag ccacgaactt
    21241 ttacctgttc ttcgttgcgg ctgcctgact tttgcagcac cacgccgggg acgacactta
    21301 atgcctgagc gacattctgt ttatccaagg ccttcatggt gggttgatcg agaacggtgg
    21361 tcgtcgtcga tgataccgga ctggaccata cggtcagcgt gtcgctctcc tgagccgcta
    21421 cggcctgctg ggtaaaggca agtgataatg ctgtgcagag ataacgtttt ttgagtctca
    21481 tagaataaat tccgtttaac aaagaattaa atagaggctg tgggttgcag actgacttta
    21541 atgtcagctg gtgccgcgta ataaggcgcg gaggtaataa aaaggcgaat gccgcagtcg
    21601 aggtaatttt tgagtgtcgt cagattaatt ccgccggtca gagccagcgt gcagtgagga
    21661 gccagcgacg gcgctatttg cgcaatttca gttgcctgtt gcggactaaa tttgtcgagc
    21721 tgaagtacgt ctggttgtgc gcgtaacgcg gcgatagcct ctttcggcgt gtcggcttca
    21781 acaacaattt tcttctcggg tgcgtggcga cgtaactgag cgattgcgcc tgaccagccc
    21841 tgattgtcat gaagaaaatg gcggtggttg gcaaacaata atatggtttc cgcacatccg
    21901 gcacgatgaa ttagtcctcc ggcagccaga attgcctgcg aagccagtaa gcgggtgccc
    21961 ggaattgctt ttcgggtgca ggcgatatta ccgtcaggat aacgttcacg aagcaatgcc
    22021 agcatttgag cgagataatc agaaacaccg caactccact ccagcacatt ctggactgcc
    22081 ttccatccct gatgaagtgc tgcggcatta ccctgcgcac ggattagccg ttgacccgcg
    22141 ttcgcttgtg aaccgtcgct gaccgcgtca tcaatagtta atcccagcgt agttaacatc
    22201 ttacacgcga cagaaacgcc gctgacgcag ccgccctgac ggtgaaaaaa cgctatatag
    22261 ccaggctggt gtccaatatt taatgctcgc gtggtcaggt cacctccctg gatatcttcc
    22321 agcagtaacg catcgatttg tgcctgagaa aggaaaatca tcacagtgcc tcctgcggta
    22381 cgctatccca ccagatgagc gcccagtcgc ggctggcagg ggcgagggca cgagcatctt
    22441 gttgttgata ccagtgatat aaccgctcac gctcatcgtc gttaagcgcg ccaagactcc
    22501 agctgacgtt ctgctcaaaa cgttcccagg tgctgttatc ctgttggcaa ttttgtccac
    22561 ggataaaatc aacgtgggca taaattccca tctgatacag gacgttgagc gcaaagatat
    22621 agttgggtaa ttcgatcact tcccgaccag ccgcccgctg aatggctgga gagacgaacg
    22681 aggtgctgac cagatgagtt gtatagacgc gaagccgtgc ctgattattc agtttgctca
    22741 ttgcatggcg catatcagcc accagagtgg agcgggaagc cacggcaata tcgcagcgtg
    22801 gcaggtcact ccagtcctct tcccaggcgc gctggatcca gtggacattg tcagccttca
    22861 gtgcggcggc gcggcgggcg gcaacgttca gcatcccctg actgtaatca acgccataaa
    22921 ccgtagtgag tttgtcagcc agcgccagcg atacggtgcc tggtccgcag cccatatcga
    22981 ataaggtctg agcgccttgc aggtcaattt tagcgatcag ttgttgcagg tagctgtccg
    23041 ttggcgaggc gcaattttcc gccattttct ccgcccgttg atcccagtga tcgggcgttt
    23101 tttctgtgcg gtgagccagt ttgagttgct gcagataaag gtctgcaaaa tcaatatcat
    23161 caatgagcat gattgttcct tagcggctaa ttgcagagag gtgatgatga atttggtcgg
    23221 cagaaacccg atacagggcc gccagtttgt tcgttgttaa ctgttcggtc ggtaagcctt
    23281 gtgttactcg tccatctggc tctacttgga taatgctgtc ggcaatggcg ttggcatgga
    23341 gcggatggtg agtcgacatt aacattgtca tgccgttatt tttgagctgc gccagcgtgt
    23401 ccagaagttg gatctgatga ccgaaatcga ggctggaggc gggctcatcc agtaacaaca
    23461 agcgcggttg ttggacgagt gcacgggcga ttaaagccag ctgacgttca ccgccgctaa
    23521 gcgtattcca gcgtcgtgtc gccagatgca aaatgcccag cttttccagt tgctccgtgg
    23581 ctttcaggcg ttcttgctta ccgggaacgg aaaaagcgcc tatggtgggg gccaggccca
    23641 tcaacaccat atccagcaca ctgaaagcaa aaataccgtc gtgcgcctgc ggcacccagg
    23701 caatagcctg tgcgcgctgg cgatggctga gtgtctgcac agagaggcta tccagtagga
    23761 tctcgccttt taataacggt ataacgccaa gaatggagcg catgagtgtg gttttgccgc
    23821 aaccattcgc gccaagcaga caacaaattg ttccttgcga cagggtgaag gacacgtcgc
    23881 gtagcactgg atggcgggag taacccagag acgcatgacg aacggcaagc aaagtcactg
    23941 gcgacctccg cgtaacaaca aaagcaggaa aaacggtgcg ccaacaaaag cggtcagaat
    24001 gccaagtggg atttctgttg ttccaatgct gcgcgccagc gtatcggtaa gcaggagcag
    24061 aatagcacca gtacacattg ccataggcaa cagctgctga tggttatgac cggtcaacaa
    24121 gcgggcaatg tgcggaacga ccagaccgat ccaaccaatt atgcccgcta ttgctaccgt
    24181 gctggcggtg attagtgtgg cgcagacaat caatccaaag cgcagacgcg tcacgttcag
    24241 tcccagcgaa cgggcttcgt cgtcggaaag cgtgagtaaa ttcatgcgcc agcggagaaa
    24301 aaacaggggc aaggagccta tgagaatgat cggtgcagca tagcagagat cgcgtaaggt
    24361 cacggtggac agtcccccca gcaaccagaa ggtaattgac ggtaactggg tgtaaggatc
    24421 ggccagcgtt ttaattagcg atatccccgc tccgcacaac gttcctaacg caatgcctac
    24481 cagcaccagg gttaagatcg gatcgtggcg ggtcacccga cgcgtaatca accagacgcc
    24541 cgcgaccacc atcagcccgc cgcaaaatgc gtacagctgg atgtaaacaa tgggtaatcc
    24601 ccacaggatt gccgcgcagg caccaagtcc ggcaccggcg gagacgccta aaatatccgg
    24661 tgaaactaag ggattacgca acattccctg ataggtggtt ccggctcctg ccagcgctgc
    24721 gcccagtaac agcgcagcaa gaatacgcgg cacgcggatt tgccagaaga caatttgttc
    24781 ctgcacagac tcattttctt gacgaagaat tagcgccagc agttgctgca tatcgagatg
    24841 ataagcccca cttacggcgg caaaagtgat actgatagct gccagcagca aaatacatcc
    24901 gtttacgatt ctcattagct cgccaccaat ttttgatagt cggcatcact gagtgaacaa
    24961 tgccagaaca gttgggcgta atgctgcatg tcacttttaa actggcgatt gatggcggga
    25021 tccagccagg cgtgaagtct gcgtaatccc agaagacggt tgattcccgg cggggcatcg
    25081 agccagccaa agggcaagcc acttaaaaat aagatgcgtt gttccgcaac ggctttcacg
    25141 ccctgccaga gaggatcacg acgaataaaa tctgcagtaa cggcctcctg aaccagaata
    25201 atatccggct gccagcgcag gagattttcc atggaaacct gtgtcaggcc gtggcgatcg
    25261 gctatttgcg cgacgttgtg caaacctaat aattccgccg cctcggtatg caacgaaccc
    25321 tgtaagcccg tttccagccc tcggggaccg cgcgcagcat aaaagcggag gttagcggcg
    25381 ggtgaggtgg cgaatgcctg agcttcacca acgaagtggc tggcgagatt ggcttgttct
    25441 gcggcgcggt gctcttcgcc taacgttttg ccaagcgttg ttaactgttc tgctgattgc
    25501 gccagttttc cgttaagcaa taaccagggg atttgtgtct gttcgctaac ctgccgtgcc
    25561 tgggagatca aggtttcatc cgtattgccg caatcaacga ccaaatcggg atgtaacgcc
    25621 ataagccctt ccagcgatag tgtgctggcg cgcccggcga gtcgtcccag cctgggtaac
    25681 tggcgaatgt gctctggcaa tggaattaat gcctgacggg caaaatcaaa agaggaaaaa
    25741 ccgaccattt tttctggcgc gacagccagc aagagcaaat cggcgggcgc gcctgcgctg
    25801 acgatgcgtg taatgttttg ggcttcggga agagattgcg aagcaaaacg attaactgtt
    25861 tgtgaaaagg acggtagaga ctgcaggagc agggtagatg caagtgcctg cgcaaacgtc
    25921 cgtctggtta tgggcatgga gaacatcctg aaaagtaaaa tcgatatata ctttcatata
    25981 tatcgataaa aatcatgttc aatatcaaag tttagctatt gaaaacatga agatgtgacg
    26041 acttccgcaa gcctgttgac cctctctatc attaaaaatg tatttatcag gtggctaata
    26101 ttatttaaat catttaaggc taaatcattt aaatatgaat gacattattg tttttttatc
    26161 gtccacggtt acgcgacgtt gaagttactt gttttataat gagcgatttt tgatatctcg
    26221 ccgagaaaat tatcagcatt ctctcgtttc agtgtcgtga atatactgtt aacggaggtg
    26281 aaaagaatgc tgggggttat acaaaaccta tgttgttgtc gtccggattc accgcttgct
    26341 gatagtaagc agcgtaacca gaagttaccg caacaagact ttgaggattt taagaacgac
    26401 gttattaatg tcattaacaa caatgaagat atttattttc ttagagagag taatttactg
    26461 aatgcgttta ttcggacgtg caaaaaaaga gatgataaca gttacggaaa tatgatattt
    26521 acgctttatt ttgataacga tatctatggg aacagaatta tcaaagcatc gctgaacggc
    26581 ggagagttgc agttattaag cacagggtgt agcaatgata acattctc
//
LOCUS       DAJVRP010000056        26077 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVRP010000056 DAJVRP010000000
VERSION     DAJVRP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.056"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OQN12_002350"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OQN12_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1654648.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OQN12_002351"
     CDS             complement(2138..2584)
                     /locus_tag="OQN12_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1654649.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OQN12_002352"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OQN12_002352"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1654650.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OQN12_002353"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OQN12_002353"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1654651.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OQN12_002354"
     CDS             complement(5347..6009)
                     /locus_tag="OQN12_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1654652.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OQN12_002355"
     CDS             6182..6595
                     /locus_tag="OQN12_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1654653.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OQN12_002356"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OQN12_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1654654.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OQN12_002357"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OQN12_002357"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCS1654655.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OQN12_002358"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OQN12_002358"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1654656.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OQN12_002359"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OQN12_002359"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1654657.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OQN12_002360"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OQN12_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1654658.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OQN12_002361"
     tRNA            complement(9861..9948)
                     /locus_tag="OQN12_002361"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OQN12_002362"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OQN12_002362"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1654659.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OQN12_002363"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OQN12_002363"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCS1654660.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OQN12_002364"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OQN12_002364"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCS1654661.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OQN12_002365"
     CDS             14005..14592
                     /locus_tag="OQN12_002365"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1654662.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OQN12_002366"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OQN12_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1654663.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OQN12_002367"
     CDS             14984..15841
                     /locus_tag="OQN12_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1654664.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OQN12_002368"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OQN12_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1654665.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OQN12_002369"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OQN12_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1654666.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OQN12_002370"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OQN12_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1654667.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OQN12_002371"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OQN12_002371"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1654668.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OQN12_002372"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="OQN12_002372"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS1654669.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OQN12_002373"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="OQN12_002373"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1654670.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="OQN12_002374"
     CDS             complement(22898..24037)
                     /locus_tag="OQN12_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS1654671.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25243)
                     /locus_tag="OQN12_002375"
                     /pseudo
     CDS             complement(24083..25243)
                     /locus_tag="OQN12_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25322..26017)
                     /locus_tag="OQN12_002376"
     CDS             complement(25322..26017)
                     /locus_tag="OQN12_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1654672.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
BASE COUNT         6217 a         6648 c         6906 g         6306 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
    25741 ttctgcacaa atcgccgact ctctgccctg taacgacagc caaatccatt gctagcctca
    25801 gccagattgg gagaggttcg gctgcatggt aaatcgcgac atactcatta gctgtcagcc
    25861 ttgagcgcct tacttctgac tttgctgtgc gggttgctgt taccggattc gttgctacat
    25921 gcccctcggc tattgcctca cgaaaaacgt caacaagggt tgacctgatt aattttgcgg
    25981 aagccgcttt accttctgct acgtaggtgt ttagcattgc tgccacctct ttcgttgata
    26041 tgtcagcgag cggtttgtcc ggcaattttc ttcggat
//
LOCUS       DAJVRP010000057        25390 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVRP010000057 DAJVRP010000000
VERSION     DAJVRP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.057"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(397..684)
                     /locus_tag="OQN12_002377"
     CDS             complement(397..684)
                     /locus_tag="OQN12_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654673.1"
                     /translation="MIKPRKSVKAPEVKDLDLERRIEAFASKADLVPGEQPEDNKVLD
                     KDAPRDFKSIRVGFNEYEYQVLDALSKKHNRSKLNMIRHAILMLAESEEAK"
     gene            complement(677..1318)
                     /locus_tag="OQN12_002378"
     CDS             complement(677..1318)
                     /locus_tag="OQN12_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000585023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1654674.1"
                     /translation="MIILVASQKGGCGKSTTSVNICAELARANKDVVLLDADKQGTSA
                     RWASDRNAAEVSPVIHCVQKFGNIRETLLDLDKRYEFVVVDTAGRDSKEMRTGITAAD
                     VLLVPFRPSQPDLDTLAHFVEVFEEALDLMPNPSIKACAVLTMAPSNPVVNEANEAKE
                     YLAEYPQLKLLKTIIRDRKVYRDCMAEGKGVVEMDNGKAKGEIQMLVKELLSD"
     gene            complement(1606..1944)
                     /locus_tag="OQN12_002379"
     CDS             complement(1606..1944)
                     /locus_tag="OQN12_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HCS1654675.1"
                     /translation="MSQERKKVMIYLRPEAYANEKAASEKIKKHSDMARTALLAGLAL
                     GEVDSRLPGLLASLLTEDNNPELIRKMLASFLELPAAVEERPASIEPVKEQVVAKSAS
                     ARNLADSLPD"
     gene            complement(1958..2914)
                     /locus_tag="OQN12_002380"
     CDS             complement(1958..2914)
                     /locus_tag="OQN12_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCS1654676.1"
                     /translation="MRIFIDDGSTNIKMLWEHDGETRTHISPNSFKRGWSATFGAGKP
                     FNYVIDDEKYSYDLISPDVLPTNNVEWQYSPLNVLAVHHALLTSGIEPQEVEIVVTLP
                     LAEFYDDDAQYNLDNIERKKASLMRPVTLNKGNVFTIKKVTVRPESIPAGIGLCDNLN
                     PAHSVLIVDLGGTTLDVSMVAGQMTAVSRVFGDSNLGVSLVTREVRQALARANTETSN
                     YNVDQLIINRHDEDYLNDNINDPSAIDDVKKAIAASIDRLRTRVLDVIGDFKGYTHVM
                     VIGGGAPLVADAIREQVNIRDDRFFVADDPQLALVHGLKAIG"
     gene            3181..3465
                     /locus_tag="OQN12_002381"
     CDS             3181..3465
                     /locus_tag="OQN12_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1654677.1"
                     /translation="MSAIATSDTLCSGHGGFPPRLVAETVPWFTVNGKPIVVDGAMFP
                     SHTDGNSAHPGAAVSTRVWFSIGGKGVVCVGDPLSCGSVIANGEDIFQVA"
     gene            3465..4268
                     /locus_tag="OQN12_002382"
     CDS             3465..4268
                     /locus_tag="OQN12_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654678.1"
                     /translation="MLDSIGEVKAFKILSDAGISTPESITISRAASVKASSLASAIQG
                     IVHADKTYPDSVSAWTTQLLSFSEQLNEASKASSLLADSLSPYTKPSELLQMKIGWEC
                     YVKGNELTPIPAFALVEGMGNVSIPQSLTDALTALKLDALKTAMNAINAKIEAAGSAG
                     GDSNSGQGGAGGAQAPVITQDEIDALREAVTAAEVLLSEINSASESVVALTGRIKTST
                     TQATKGLENAVAITLTGSLLDDAVMSPAISLIMPQGVIDALQKNTKKEQ"
     gene            4339..4797
                     /locus_tag="OQN12_002383"
     CDS             4339..4797
                     /locus_tag="OQN12_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654679.1"
                     /translation="MVLKKIILIVIALLAISGIYRTTTAVIEHIRYVRSLEDSKSQLE
                     STNNTLSTALQLSENARSAMLTENERIKALEKEYQRKTAELNANLIKQREESQNEITR
                     LENALRRAGINDVRVPDDVIRMQRERAKAINQRASENYRRSHQQAAGKSD"
     gene            complement(4951..5409)
                     /locus_tag="OQN12_002384"
     CDS             complement(4951..5409)
                     /locus_tag="OQN12_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654680.1"
                     /translation="MSHVNPSKTQYRLMLAIASAIPTSLNPPTGYPAVVDDCFQYYGE
                     DILSQSKALKQLCKAGILHCIGDPDDFVVMLADRDSFLLSWKAGAREARLGNGIGYID
                     YSDCPLAFAGGYMHWHERNRGRQRQYRLSDFNVCHGFEEADSQDIWLQEP"
     gene            complement(5426..5617)
                     /locus_tag="OQN12_002385"
     CDS             complement(5426..5617)
                     /locus_tag="OQN12_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654681.1"
                     /translation="MESIKWPVCGCPARVEEPVTATLYMPKAVRDWLAMVGNGKVSAG
                     LRKLIEMADIPELKNAWRQ"
     gene            5977..7674
                     /locus_tag="OQN12_002386"
     CDS             5977..7674
                     /locus_tag="OQN12_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HCS1654682.1"
                     /translation="MNKNIQPARDREREIMNRAASVMAMTVDPTTDAAGNMIADQAVM
                     MENLDKAIQKVPMFEGVNPEVARQITGGWAMSLHEYKRQHGHYPASDILANAHLALER
                     LMTECASDTHEGTGKAMFESVAQSMRSSDGVMKVAQYAALILPASLGAATSDACTFVP
                     CDRDESNIYELVNVAGTKFGTFNQGDELNMQSAGVYSQMKRLYALSTKGDGSTKTFQF
                     NIKQFEGQSCPLRAGYNKLLINRKPSKVDDGDGNLYFNAKDSKGNTFSATAKVAYDTG
                     VIDITFTQAPPVGTEIAVQVEINIERNPSLIPVINQAMRKYEVRPSQYVLASEHTVMS
                     ASDLSREHGLELAALQFSAMRNWISHETDIMRLRTLVFHTVYGREFDVALPEAQNYES
                     WVGLLRHAVNALSQEMANRTLTTGIRGGFAGSDAANFLRSLPPQHFQIAPGYVQSPYV
                     QYIGTLFGTIRIYEVPQPVCEQFLAQGYDFGLDDIFFYGRGEGIGKAGLIAGDAVPAI
                     PYVHETNPSLVNRTTLWGSAINEVHPRNGENYFTRLRLTRAKEGAIDMLTGKVNEKK"
     gene            7821..8099
                     /locus_tag="OQN12_002387"
     CDS             7821..8099
                     /locus_tag="OQN12_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1654683.1"
                     /translation="MANIQVTITPPDATDKSFTVESDHPDIVQVDGTTCTALKAGEAV
                     LTIKTNDGNKTAQCTVTVREAHKSVSTVSVEPTTKEVTVGDTFTVSES"
     gene            8167..9825
                     /locus_tag="OQN12_002388"
     CDS             8167..9825
                     /locus_tag="OQN12_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1654684.1"
                     /translation="MNKISFSVGQAAGVAVMSVNADATLTNTSGGASVFAGLVISRRG
                     APGKVLKVDDTTYQSVLGSPIHPRLGAAFEPYRHVERAVKGGSGYVVRVCAKDMKVPG
                     ISVSVVGKAKAAKASKELSVEPTETTANLGDTLSVGVAPTATTQSVTFTPKETPQIKG
                     GEKALFFIKDGDASQNRTLSLTRDDEESKLFTLTLKEKQTDGSIEVLESHQVSFNPEG
                     TNDMGQPAWIPTLLESQSTRIGAVLADNAEASAAQLIFEDVAFEGGTDGNLSEIAAAD
                     YLEALKVLEASEVNYTALLSLGCYDASALAAIKKLAEDVRVDMFYDLKGNQTPENAIS
                     EAKSHSFGGSHQPSRYYFPLSCRDTFTGMNVVYGISCDAFVAKAKGVALVPDVGGWHY
                     APAGISRAIIDRQNIARIPNIGAVDREAFVLARINPVSVAADGSVYIDDSLTTYSKNN
                     YLRFQHVSSLMNAIARNFYEVAQAIKHEPDGITKEALMKAMTELLDRYVAAGALVTPR
                     DKSQGEDPYVVQVVQKDIDLWEVAWSVCPTGTARRIVGKPILMR"
     gene            9869..10603
                     /locus_tag="OQN12_002389"
     CDS             9869..10603
                     /locus_tag="OQN12_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654685.1"
                     /translation="MKNYTADPFMRAIFGSGCFESGDKSTQDNNDNAMLESAGQKGKD
                     TQPKPKDDIETAMMEAVESRAQGDMRSLAASMLAGWVEDGDPEADSFDALAITMAGLA
                     DIDEGTDFTDEQIDAYNDALAALADAAVALGADQDDVTEMIDDEDDSAAERVFDALSE
                     SDTDMMETAIAIYTVAGGDSAMLEAVRKKVVRDGKVTIIRKRPRPRRMTSLQKQALKK
                     ARRKAHTSVANINRKKSMRIRKKRGL"
     gene            10671..11243
                     /locus_tag="OQN12_002390"
     CDS             10671..11243
                     /locus_tag="OQN12_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654686.1"
                     /translation="MPDGISPLMKVYILSSEDMVVGYIGEGSTAELSSMWQSPFENQS
                     VGGLLGGISAAAGSLADTLQTATGVTTKTLFNSMLVWEGQQPPEFNLVIDFMATTNAQ
                     LEVNAAITALLKMESPELNNVAPFGRRPETVTLNIGRNIALTDVVIKSVSYQLDAPRT
                     PEGYFTHNTVTLQCSGNTSINRSNISSVFV"
     gene            11252..11743
                     /locus_tag="OQN12_002391"
     CDS             11252..11743
                     /locus_tag="OQN12_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS1654687.1"
                     /translation="MSGFANTKADIAFLKSRFNKNLAAGEKLIGSEFWMTIKGYENLS
                     VLVRTTQLPEMTREDVEDYAPGGMKFNQHGPLRNSGEFQVTCSETIEGDVLAAVKQMV
                     YGKEYLDITFQAAAESNSGNHKGLIRTYSHCKVYSDAVDFSSEDVTAVVKPSLRIVYN
                     WVE"
     gene            11798..12349
                     /locus_tag="OQN12_002392"
     CDS             11798..12349
                     /locus_tag="OQN12_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654688.1"
                     /translation="MTPTELLESVKTRFNPLLVREEDTLKAFLIKALTTYQDRAGVVK
                     TLKLEKTGGTAIPLPEDYLSLVHVTDSNGLLVYSDELSGFIELELTGSERWPFRMLYL
                     VNLRDRKLDEWQVPPAIIGMLEEYLEALINVRNVARQRRASIDGKFDYSDLPDEATLY
                     ARVQEIEEKMSSNRAIIPGATIF"
     gene            12365..13072
                     /locus_tag="OQN12_002393"
     CDS             12365..13072
                     /locus_tag="OQN12_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1654689.1"
                     /translation="MSIFSSVGRTLTTALSFNTKSFTSNLISDILDKAISGGGVSGNY
                     SSDIAYGKNIVAAAMRIRYAQGWQWTVEVDGLNGFDMFVKDITYSSGNIETENKVIGS
                     VEFSKPTYVTAGPVTMTVRDTEDGKIMNWFKERRSRVTNPDGTINLPPEYLMKIRLYR
                     VTQDGGKELEEEMRVFPTQLGEITRSRDQVSEFLSYPITFQKYTSAGSGVSALVNGAA
                     GMATSALKGAVSGVIKF"
     gene            complement(13454..14209)
                     /locus_tag="OQN12_002394"
     CDS             complement(13454..14209)
                     /locus_tag="OQN12_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCS1654690.1"
                     /translation="MNRAELTAKAVSLIESATPISRSLAQANEITEAAYHLTRDQKRL
                     LFIVVGRLRYASKDGVLGSGACELTVNEYAEMYNLPSAEASKDIRKAISGLSEKKVTI
                     YNPDESTESEDSYESYPWMIKDAYSPRRGTYIIHLNPYLMPFFTLLDKRFTRLNFTEV
                     SRLTNPYSMRLYESLCQYRKDDGSGFAILGVEWMRERYGLPKSYQRYAEFKRSFLTKA
                     VAEIEKNTKMKIVFSEVTEGGKVTRIKFTYQQS"
     gene            14598..15419
                     /locus_tag="OQN12_002395"
     CDS             14598..15419
                     /locus_tag="OQN12_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654691.1"
                     /translation="MNIPKFPLPSRPETEIQFHAPTVKDALKYSDLNPAEDEATTTEY
                     LNSMQDGEINDSANWTVQDRRTALWWIFVNSRPDAVMTYSYECSHCGNTHHADINLSD
                     LAQTVEILTVPPYVKTNVPVNGVPTDWILKPLTGKGAELLERMRASLPDMKSPEYSAG
                     VARMRIAELALCTALKDDPEDFTQAANRRFDIIESMALETEFTPLVARIQLMQKDLRH
                     GLKMSIERGSSRLILPPQHCKNAKEGADVTTTLYVPFLNREFIPSIRSEWMANHY"
     gene            15567..18503
                     /locus_tag="OQN12_002396"
                     /pseudo
     CDS             15567..18503
                     /locus_tag="OQN12_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
     gene            18975..19604
                     /locus_tag="OQN12_002397"
                     /pseudo
     CDS             18975..19604
                     /locus_tag="OQN12_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
     gene            19610..19984
                     /locus_tag="OQN12_002398"
     CDS             19610..19984
                     /locus_tag="OQN12_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654692.1"
                     /translation="MLYEIDARLQTNESGVIIAEGSTAAWMARLDEWLRTPEGSVYGL
                     PSWGSPMEEFKHEPFGSETSHIVEVAIEGRMMKKLRQDLPGLDVQGIRCTSISEDSLL
                     ISFYAKGGSMDIVMQKSSGVGA"
     gene            19981..21411
                     /locus_tag="OQN12_002399"
     CDS             19981..21411
                     /locus_tag="OQN12_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS1654693.1"
                     /translation="MTITELLDKFNAKLNENTWWSRFVNSQFVQMHAIFGSQLIYIAR
                     TFASRGLTEGLISTATRRSSILAVAEDRSYVGRFVSASYGTTSITNKTDRDITLPAGA
                     ELLANDQTPLAIINSVVIPAGGTISGVETKQHEAVSITFDIEKETLFLTLLLSRELTK
                     EVSSLDVYVITDGVEEKWTYNPLFRMSRDKSKHYSLAYKPTEQLGVKFGDGSMGMMPP
                     AGCQVRIDVMASLGDYTLAEGQKLEPAGNIAQYVESLEFKTDSIITGGSGMETTEETR
                     NRAQYYVAYDEQVVWGGDYRQFIQNVVHGTSWLNVWGEALQEKITGFDVRNINKIFFC
                     GHKPGVSQAQLKSEILKALENVPNELNKRFEYVDTNEQPFTINFIGIVRKNVLIDDAQ
                     NAIKAALEDNFGRDSSSFSLLLQSDDDSQQCYAQVKVKDIWRVIESLDMFLSYDITIQ
                     NMKDAVYFNDFIYLNVKSSTFSISYP"
     gene            21422..22267
                     /locus_tag="OQN12_002400"
     CDS             21422..22267
                     /locus_tag="OQN12_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1654694.1"
                     /translation="MKDNWLKERLTKVKQDSHLWSAFIDALQDVWNEEVEPILTRISN
                     RKSFFTMDSEDMDARIAEYGRFFVITEKDKDRRPMLLAQRLDEVHFKGTILPIEQTFW
                     REFGCIPVSWEPLYAPVNIEKHPYGSYFATEIEIPTAQAQFGEFFLTSRGLVVVDQNK
                     LYRSYGEQDKEAAVQKLLSDFETVIAPLLPLHIVFDGVSFRLSAVFPEVAEILNCLST
                     DVSVIEGVYVTESMADMLSHSDTSSQVDNISLNAIPNRTAEKQLHLDVTPLDAWPLDY
                     HLQPV"
     gene            22362..22808
                     /locus_tag="OQN12_002401"
     CDS             22362..22808
                     /locus_tag="OQN12_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1654695.1"
                     /translation="MAENLKVRDGNRLYKAQLLSYYYSRRAESAIGKGARFVISKAYW
                     CKSSLVTANGAGGWNIADIPLNFKLSNAQQFAVSDLILSSVDGIITINAAFPQEKMPD
                     NTPYDFNTLVLVDAEEQAFAVLCTQQDTLYKGKRYSILMTIEQVEG"
     gene            22838..>25390
                     /locus_tag="OQN12_002402"
     CDS             22838..>25390
                     /locus_tag="OQN12_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCS1654696.1"
                     /translation="MTLSSGVSQPLLADVQYFELYSSSALNRKLKNIVLPGFYCGFEP
                     VPGTGLSVRITSENSEGKGAASVDVNNVQISVQQIEDVTVSVKAGATNIIVLEANFEH
                     GVKTTQVDSASSVSAARIFARTDNAIGQNQIELCRVIVPGGATAVTKEMIVLKYRVNR
                     AVGVEFSNEISSTEERKAATPLAVKTLHDLVDTKAPLNSPHLSGTPTVPTASQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNYSTTINNALALKAPLASPAL
                     TGVPTAPTAAQGTNNTQIATTAYVRAAISALVGSSPEALDTLNELAAALGNDPNFATT
                     MTNALAGKQPLDATLTALAGLATGANKLPYFTGKDTVAQTDLTSVGRDILAKTSTLAV
                     IQYLGLRELGTSGEKIPLLSTANTWSARQTFNGGITGALTGNADTATKLATARNINGV
                     KFDGSVDISIPTITSRGRVTALTGTTQGAATGLQMYEAYNNGYPSAYGNVLHLKGATA
                     VGEGELFIGWSGTSGAHAPVHVRSRRDTDTASWSEWAQVYTSKDSIPGVNTTGNQNTT
                     GNAASATKLQTARTIGGVSFNGTANIDLPGVNKTGNQSTTGNAATATKLQTARTINGV
                     SFDGTANISLSPANIGCPASPTGWLVTGDNGASITTEQLVTLLRDNGAFNAKAWIARC
                     AWAYANSASIPDSETGCGIIPLAGAVIEVFNNGNSSNNYTIRITTATTTGVSGALTNA
                     EFVYVFNGTSYSPGWRRAYNTKNKPTAADVGALPLSGGALTGGLTAAGEIISKSANGL
                     RIAYGNYGFFIRNDGGNTYFMLTASGDTLGSWNSLRPITINNTSGVVSIGNGL"
BASE COUNT         7329 a         5837 c         5955 g         6269 t
ORIGIN      
        1 ccaatatgca gatagccatt cggttccggc gggaaacggg tatgtactgt ggtgtgctta
       61 ccacttgcca gatcttcatc gatgatctga cgaataaagt tagtcgggcg ggcttctgcc
      121 tcactcattg tggattcctc aaagcgtaaa aaacgtataa cagcgtatga tcttataagc
      181 cgaataggct gacaaccact agttgattag aaattgcttt ctatcgttat ttcaataatg
      241 agctttttca tgaagcccac ttttgcgcat aagtaaatta atgacaaatg gtacacaatt
      301 ggaaccaaaa aggatgtaaa ggtgcaccaa attagtagtg tattggtgca tctttggttc
      361 aagatttttt aagttctaaa accaccctga aagactttat tttgcttcct cagactccgc
      421 taacatgagg atagcatggc ggatcatatt caatttgctg cgattatgct ttttgcttaa
      481 cgcatcaagt acctggtact cgtattcatt gaagccaaca cgaatggatt taaaatcacg
      541 tggggcgtcc ttatcgagca ctttgttgtc ttctggttgc tcacccggca ccaaatcagc
      601 tttgctggca aaagcctcaa ttcggcgttc aagatcgagg tcttttactt cgggggcttt
      661 taccgattta cgaggcttaa tcacttaata actccttaac caacatctgg atttcacctt
      721 tagctttccc gttgtccatc tcaacaacgc ccttcccttc agccatacaa tcgcggtaaa
      781 ccttgcgatc gcgaatgatg gttttcagca acttcagttg cggatattca gccaggtact
      841 ctttggcctc attggcttca ttcacaaccg gattggatgg ggccattgtt aagactgcgc
      901 acgccttaat gctaggatta ggcatcagat ccaaagcctc ttcaaagact tcaacaaaat
      961 gcgccagagt gtctaaatct ggctgagatg gcctgaatgg aacaagcaaa acatccgccg
     1021 cagtaatacc ggtgcgcatt tctttgctat cacgaccagc ggtatcaaca accacaaatt
     1081 cataacgctt gtccagatca agaagtgttt cacgaatatt cccaaacttc tggacgcaat
     1141 gaattactgg agaaacctct gccgcattac ggtccgatgc ccaacgggca gatgttcctt
     1201 gcttatctgc atcaagtaga actacgtcct tatttgcgcg ggcaagctcc gcacaaatat
     1261 ttacgctggt ggttgatttg ccacagccac ctttttggct ggctaccaat ataatcatat
     1321 ccgttcctcg gtgtgatttt ggtatcaaaa cggttccaga taagagcctt tttgatgcgg
     1381 gacaggctct ataatggagt catgaatgtt tggcaaagta taacatcaat cagaccaaat
     1441 acaccttata ttgcatcctg tttaacacga aatcagcccc aaattggaac cagatacaaa
     1501 actgcaccat aacagcacct aactggttcc aaattaacac caattagaca ccattaatgc
     1561 ataaaaaagg ctgcaatttg cagccttaaa ccagcacatc ttctgtcaat caggtagaga
     1621 gtcagccaga ttgcgcgccg acgcgctttt ggcaacaact tgctccttca ctggctcaat
     1681 ggaggcaggg cgctcctcaa ccgcagctgg tagttccagg aaggatgcca gcatttttcg
     1741 gatcagctcc gggttattgt cttcggtcag cagggaagcc agtaatcctg gaagcctgct
     1801 atcgacttct cccagcgcaa gccctgccaa caatgcggtt cttgccatat cgctatgttt
     1861 ttttatcttc tcgctcgccg ccttttcatt ggcataagcc tctgggcgaa gataaatcat
     1921 cactttctta cgttcctgag acatggacat tacctcgtta accgattgct ttcaggccat
     1981 gaacaagagc aagttgcggg tcatccgcca cgaagaaacg gtcatcacga atattaactt
     2041 gctcgcgaat tgcatctgcc accagcggtg cgccaccacc gatcaccatg acatgcgtat
     2101 atcctttaaa gtcgccaatc acatcaagaa cgcgggtacg caaacggtca atgcttgcgg
     2161 caatggcctt tttcacatca tcaatcgctg atgggtcatt gatattgtcg ttcagataat
     2221 cttcatcgtg gcggttaata atgagctgat cgacattgta atttgacgtt tcggtattgg
     2281 cccttgcaag tgcttgcctt acttccctgg tgaccagtga tacaccaaga ttcgaatcgc
     2341 caaaaacacg ggaaactgcc gtcatttgcc cggcgaccat tgaaacatca agggtagttc
     2401 cacccaaatc gacgataaga acagaatggg cagggttcag attgtcgcac aggccaattc
     2461 ctgccggaat agactccggt cgtaccgtaa ctttcttaat cgtgaacaca ttgcctttat
     2521 tcagcgtgac ggggcgcata agactggctt tcttgcgctc gatgttatcg agattgtatt
     2581 gcgcgtcgtc gtcgtaaaac tccgccagag gcagcgtgac cacaatttct acttcctgcg
     2641 gctcaatgcc acttgtcagc agggcatggt gaacagccag gacgttaagc gggctgtatt
     2701 gccattccac gttattcgtc ggcagaacat ctggcgagat caaatcatac gaatactttt
     2761 cgtcgtcaat gacatagtta aacggcttgc cagcaccaaa tgttgctgac catccgcgct
     2821 taaagctgtt agggctgatg tgagtgcgag tttccccgtc gtgctcccac agcattttga
     2881 tattggttga accatcatcg ataaaaatgc gcatcttctt gctccacttc tgagacacat
     2941 ttgagaacca aaatgcaatt ttaaaagaga agcacccaaa aatccacacc aaaagagata
     3001 aaaaggagtg attattgagt taattttgag aataccttaa tcacccaatg aattatggtc
     3061 tgacagtgag atttatttga gaatgaagtg agaatacaga aatattccga aatttaactc
     3121 tcagtttgaa tgacgaaata acaaagtgat gacaatagga ggataacccg gaggtgaaga
     3181 atgagcgcaa tagccaccag cgacaccctt tgttctggtc acggaggatt tccgccgcga
     3241 ctggtagctg aaactgttcc ctggttcacc gttaacggta agccaattgt tgtagatggt
     3301 gccatgtttc ccagtcacac tgacggtaac agtgcacatc caggggcagc tgtatcgacg
     3361 cgagtatggt tttcgatagg tggaaaggga gttgtctgtg taggcgatcc gctttcttgt
     3421 ggttctgtta tcgccaacgg cgaggatatt tttcaggttg cataatgctt gattcaatag
     3481 gtgaagttaa agccttcaaa attctgtcag atgccggtat ctctacacca gaatctataa
     3541 ccataagccg ggcagcgtct gtaaaagcct catcactcgc cagcgccata cagggaatag
     3601 ttcacgccga taaaacctat cctgattctg tttcggcctg gactactcaa ttgcttagct
     3661 tttctgaaca gctcaatgag gccagcaaag cgtcttcatt actggctgat tctctttccc
     3721 catacaccaa gccatcagag ctgttacaga tgaagattgg ctgggaatgc tacgtaaaag
     3781 gtaacgagct gacccccata cctgctttcg cccttgttga ggggatgggg aatgtttcca
     3841 ttccgcaatc tctaacggac gcattgacgg cgctaaaact tgatgctctg aaaactgcca
     3901 tgaacgccat taacgcaaaa atagaagctg ctggaagtgc tggtggtgat agcaatagtg
     3961 gacagggtgg tgctgggggc gctcaggctc cggtcattac tcaggatgaa attgatgctc
     4021 tacgggaagc agtgacagcc gccgaagttt tactctccga aattaactca gcaagtgaga
     4081 gtgtcgtagc ccttacaggt cgcattaaaa cgtcaaccac tcaggcaacc aaaggtttag
     4141 agaacgctgt cgccatcacg ttaactggtt ctctactgga tgatgcagta atgagtccgg
     4201 caatcagttt aatcatgcca caaggcgtta tcgatgcgct ccaaaagaac accaaaaagg
     4261 aacaatagca acaccacttt ggaacacaaa aggatgtaaa ttggtacatg tttaacacca
     4321 atagtaaacc tgaagaggat ggttttgaaa aagataattt tgattgtaat tgctctttta
     4381 gccatttctg gaatttaccg aacaacgacg gcagttatcg aacacatcag gtatgtccga
     4441 tcgcttgaag actcaaaatc acaactggaa agcacgaaca acacgctttc tacagcatta
     4501 cagttaagtg agaacgctcg ctcagccatg ctgaccgaaa acgaaagaat taaagcactg
     4561 gaaaaggagt accaacgcaa aacagcagaa ctgaatgcca acctaatcaa acaacgggag
     4621 gaaagccaga atgaaatcac tcgtcttgaa aatgcgttac gccgtgctgg gattaacgat
     4681 gtacgtgtgc ctgatgacgt tatcaggatg cagcgggaac gggcaaaagc catcaaccag
     4741 cgtgccagtg aaaattaccg tagaagccac cagcaggccg caggcaaatc tgattgacta
     4801 ctccccttgt ttagtgccag aagacacgcc agtgctgtta aacaagtatc cgacatacgc
     4861 tgaacagtta ttcagcacga tcgacgaatg caaccaacga aacgcactga ataaccaaaa
     4921 aaacagggag atttgggaag ggaaggggga tcaaggctcc tgaagccaga tgtcctggct
     4981 gtcagcttct tcgaaaccgt gacagacgtt aaagtcactc aaacgatact gacgctgacg
     5041 gcccctattt cgctcatgcc aatgcatata tccaccagca aatgccagcg gacaatcgct
     5101 atagtctatg taaccaatac cattccccaa acgtgcttcg cgcgcaccag ctttccagga
     5161 cagtagaaaa gagtcacgat ccgccagcat aacaacaaaa tcgtccggat ctccgataca
     5221 gtgaagaata cctgccttac ataactgctt gagcgctttt gactggctca ggatgtcttc
     5281 tccgtaatac tgaaaacaat catcaacaac agcgggatag cctgtcgggg gattcaggct
     5341 ggttggtata gctgacgcga tcgccagcat taagcgatat tgtgtttttg aggggttaac
     5401 gtgactcatt ttgtcccttg tttactcatt gtcgccatgc attttttaac tcaggaatat
     5461 ctgccatttc aattaacttg cgaagtccag cactgacttt cccattgcca accatagcaa
     5521 gccaatctct tacagctttc ggcatgtaga gcgttgcagt taccggctct tcaaccctgg
     5581 caggacatcc gcatacaggc catttaattg attccataac ttcggatgcc ggggtgaggg
     5641 tggggacatt gagtatatct ctaccagaaa actggataac gatccgttca gcgagcgaga
     5701 gtatctcact ttcggcaaga attgatgcca actgcgcctc aacaagaaag cgacttgttg
     5761 tgttcagaga gtggctctta gcgccatttt tatacgaaac aaagattcga tacgccataa
     5821 ctctaatatt ttgtgttaga aaattaaaat taaatcacat aacaacgtga ctgttaatag
     5881 attttatttt actaaaacta ttgactaaat cacaaagtag gtattgttag cccgtcaaaa
     5941 gaaccttcac gccgcgcccg aatttagggc ttaaccgtga ataaaaacat tcagccagct
     6001 cgtgatcgcg agcgcgagat tatgaatcgg gcggcaagcg tcatggctat gaccgtcgat
     6061 ccgaccaccg atgcagcagg caacatgatt gctgaccagg cagtaatgat ggaaaacctg
     6121 gataaagcaa tccagaaagt gcctatgttt gaaggcgtaa atccagaggt cgctcgtcag
     6181 atcactggtg ggtgggctat gtctctgcat gagtataaac gtcaacatgg tcattacccg
     6241 gcaagtgaca tcctggcgaa cgcacacctg gctcttgaac gcctaatgac tgaatgtgcc
     6301 agtgataccc atgaaggcac gggtaaggca atgtttgagt ctgtcgcgca atcaatgcgt
     6361 agctctgacg gcgtaatgaa agttgctcaa tatgctgctc taattctgcc tgcttctctg
     6421 ggcgctgcta ccagtgatgc ctgtactttt gttccttgtg atcgtgacga gtcaaatatt
     6481 tatgaactgg tcaacgtagc aggcacaaaa ttcggcacct tcaaccaagg tgacgaactg
     6541 aatatgcagt cagctggtgt ttattctcaa atgaagcgcc tgtatgccct ttccactaaa
     6601 ggggatggca gcaccaaaac cttccagttc aacatcaaac aatttgaggg acaatcctgc
     6661 ccgcttcgcg ctggttataa caaactgctg atcaaccgca aaccgtccaa agttgacgac
     6721 ggcgatggca acctttattt caatgctaaa gacagcaaag gtaatacttt ctcagcgact
     6781 gccaaagttg cctatgacac tggtgtcatt gacattacat ttacccaggc tccgccagta
     6841 ggtactgaaa ttgctgttca ggtcgaaatt aacatcgagc gcaatccaag cctgattccg
     6901 gtgatcaacc aggctatgcg taagtacgaa gttcgtccgt cccagtacgt acttgcttcc
     6961 gaacacacgg taatgtccgc atccgattta agccgtgagc acggccttga attagcggca
     7021 cttcagttct ccgcgatgcg taactggatc tcccatgaaa ccgacatcat gcgtctgcgc
     7081 accctggtat tccataccgt ttatggtcgt gaatttgatg tggctctgcc ggaagctcaa
     7141 aactatgagt cctgggtcgg cctcctgcgt cacgcagtaa acgctctgtc tcaagaaatg
     7201 gcaaatcgta ccctgacaac gggtattcgt ggcggctttg ctggtagtga cgccgcgaac
     7261 ttcctgcgct ctctgcctcc acagcacttc cagatcgcac caggttacgt tcagtcgccg
     7321 tatgtacagt acatcggcac cctgtttggc acgattcgta tttacgaagt gccgcaacct
     7381 gtctgtgagc aattcctggc acagggatat gacttcgggc tggatgacat cttcttctat
     7441 gggcgtggcg aaggcatcgg taaagccggt ctgattgctg gtgatgcagt cccagcaatc
     7501 ccttacgttc acgaaaccaa tccgtctctc gttaaccgta ccactctctg gggcagtgcc
     7561 atcaacgaag tacatcctcg caacggtgaa aactacttca cccgcctgcg tctgactcgt
     7621 gccaaagaag gtgctatcga catgctgaca ggcaaggtta acgaaaaaaa gtgatggtgg
     7681 cgaacgtgtc ggtatcacca acgtcacttg acgtagatga aggtgaaact atcgccgcga
     7741 acacttcaaa ggcacagaaa aaaacaaagc agaagtaagg caaaaacacg cccctgatta
     7801 aggggcgtcc aggagaaaaa atggcaaaca ttcaagtaac tatcacgcca cctgacgcaa
     7861 cagacaaatc tttcactgtt gagtcagatc accctgatat tgtccaggtt gatggcacta
     7921 catgtaccgc tcttaaggcc ggggaggcag tgctgacaat caaaacaaat gacggcaata
     7981 aaaccgcaca gtgcacagtc actgttaggg aggcacataa aagcgtaagc accgtcagtg
     8041 ttgaaccaac tacgaaagaa gtaaccgtag gcgatacctt cacggtaagc gaatcctgat
     8101 cagaggctgc ccattaaccg tgggcagcct tgcctcgttc tctcgcttta gaggataaga
     8161 accagcatga ataagattag tttttcggta gggcaggcag ctggcgttgc tgtaatgtca
     8221 gtaaatgctg acgctaccct caccaatacc agcggtggcg catccgtctt tgctgggctt
     8281 gtaatatcgc gtcgtggcgc accgggtaag gtactgaaag ttgacgatac gacttatcaa
     8341 agcgtattag gttcaccgat tcaccctcgc ctgggcgcgg catttgaacc atatcgccac
     8401 gtggagcgtg cagtaaaagg cggctctgga tatgtcgttc gagtttgcgc taaagacatg
     8461 aaggtgccgg gtatttctgt ttccgttgtg ggcaaagcaa aagcggcaaa ggcgagcaaa
     8521 gaactttccg ttgaacctac tgaaaccaca gcaaatctag gtgacacatt aagcgttggt
     8581 gttgctccaa ctgcaacgac tcaatcagtg accttcacac ccaaagagac gccccagatt
     8641 aaaggtggag agaaggcatt attcttcatc aaagatggtg atgcatctca aaatcgcacg
     8701 ttatcactaa ctcgcgatga tgaagaaagc aaactcttta cgctgacgct gaaagaaaaa
     8761 caaaccgatg ggtccattga ggtgctggaa agccaccaag tgtctttcaa tccagaaggc
     8821 accaacgaca tgggccaacc agcatggatt ccgacactgc ttgaaagcca gtccactcgc
     8881 attggtgccg tactggcaga taatgcagaa gcatccgctg ctcaactcat ttttgaggat
     8941 gtggcttttg aaggtggtac tgatggaaat ctgtccgaaa ttgccgctgc agactatctg
     9001 gaagcattaa aggttctcga agcttcagag gttaattaca ccgcattgct gtcattgggt
     9061 tgttatgacg catccgcctt agccgcaatc aaaaagctgg ctgaagatgt tcgcgtagac
     9121 atgttctatg acctgaaggg caaccagaca cctgaaaacg ctatttcaga agcgaaaagc
     9181 catagttttg gcggctcaca tcaaccaagc cgttactact tcccgctctc ctgccgagat
     9241 actttcactg gaatgaatgt cgtctatggc attagctgcg acgcattcgt ggcgaaggca
     9301 aaaggagtgg cgctggtacc ggatgttggt ggttggcatt acgcaccggc aggtatatca
     9361 cgcgcgatta ttgatcgaca gaacattgcc cgaattccaa atatcggtgc agttgatcgt
     9421 gaagcgttcg ttcttgcgcg cattaatcca gtttcagttg ctgctgacgg gtccgtttac
     9481 attgacgact ctttaacaac ttacagcaaa aacaactatc tgcgattcca gcacgtatcg
     9541 tccctgatga acgctatcgc acgcaatttc tacgaagttg ctcaggcgat taagcacgaa
     9601 ccggatggca ttaccaaaga agcgctaatg aaagcaatga ctgaattgct tgaccgttac
     9661 gttgcagccg gtgcgctggt tactccacgt gacaagtccc agggtgaaga tccatatgtt
     9721 gttcaggttg tccaaaagga catcgatctg tgggaagtgg cctggtctgt ttgtccgacc
     9781 ggtacagctc gccgaatcgt cggtaagcca attctgatgc gctgatttat aacccccgct
     9841 aaatgatgcg ggggagagag gtttgaatat gaaaaattat actgctgatc cgtttatgcg
     9901 tgcgattttc ggttctggct gctttgaaag cggtgataaa tccactcagg acaataatga
     9961 taacgccatg ctggaaagcg caggacagaa aggaaaggac actcaaccaa agcccaaaga
    10021 cgacattgaa accgcgatga tggaagcggt tgaatcccgt gcacaaggtg atatgcgcag
    10081 cctggcagcg tccatgcttg ctggttgggt tgaagatggt gatccggagg ctgactcatt
    10141 tgatgcatta gcaatcacta tggctggcct ggctgatatc gatgaaggca ccgatttcac
    10201 cgatgaacaa atcgatgctt ataacgatgc tctcgcagct cttgctgatg cagctgttgc
    10261 gcttggcgct gatcaggacg atgtaaccga aatgatcgac gatgaagatg actcagctgc
    10321 cgaacgtgtt tttgacgctc tttccgaaag cgacaccgac atgatggaaa cagctattgc
    10381 catttacacc gttgctggtg gtgatagcgc aatgctggaa gcggtacgta aaaaagtggt
    10441 tcgtgatggc aaggttacca tcatccgtaa acgcccgcgt cctcgccgca tgacgtcatt
    10501 gcaaaaacag gcgctgaaaa aagcacgccg caaagcacat acatccgtag cgaatatcaa
    10561 ccgcaaaaaa tcaatgcgca ttcgcaaaaa acgcggtttg taagtgacat aggccgcctg
    10621 caaaggtggc ctctaccagg gagaaatagc gtttgatttg cggcgcgata atgcctgacg
    10681 gaataagtcc gttaatgaag gtttatatcc tctcttcaga ggatatggtc gtcggttata
    10741 tcggtgaagg atctaccgca gaactatcat caatgtggca atcaccattt gaaaaccagt
    10801 cagtaggagg tttgcttggt ggtattagcg ctgcggctgg ctctcttgca gatacgcttc
    10861 agaccgcaac cggcgtaaca acaaaaaccc tcttcaactc aatgcttgtt tgggaaggtc
    10921 agcaaccgcc tgaatttaat ctggtaattg atttcatggc gacaacgaac gcgcaacttg
    10981 aagtgaacgc agccattaca gcattgctga agatggaatc gccggaactg aacaatgtag
    11041 cgccattcgg tcgtcgcccg gagacagtaa cgttaaacat aggtaggaac atagccctta
    11101 ccgacgttgt tatcaaaagc gtgagttacc aactggacgc cccgcgcaca ccagaaggct
    11161 atttcactca taacacggta accctgcaat gcagcggtaa tacatcgatc aaccgtagca
    11221 atatctcatc tgtttttgtg taggagtaat tatgtccggt tttgcaaata caaaagccga
    11281 tatcgccttc ctgaaatccc ggtttaataa aaacctggca gcaggcgaaa aacttattgg
    11341 ttcagaattc tggatgacta ttaaaggtta cgaaaacctg tctgtacttg ttcgtaccac
    11401 tcagttaccg gaaatgacac gcgaagacgt ggaggattat gcccccggcg gcatgaagtt
    11461 caaccaacac gggccattgc gcaactctgg tgagttccag gtcacctgct ctgagacaat
    11521 cgaaggggat gtactcgcag ccgttaaaca aatggtttat ggcaaagagt atcttgatat
    11581 caccttccag gcagcggctg aatccaacag cggcaaccac aaaggtctga ttcgtaccta
    11641 ctctcactgc aaagtgtatt ccgacgccgt ggacttctct tccgaagacg taacggcagt
    11701 cgtgaaacct tccctgcgta ttgtttacaa ctgggtagag tgatctcttt cccgtccttc
    11761 cggacgggat ttcctctgaa tttgagttgt gactacgatg acaccaaccg aattattaga
    11821 aagcgttaaa acgcgattta acccactgct tgtgcgtgaa gaagacacgc tgaaagcctt
    11881 cctgattaaa gcgctgacca cgtatcagga cagggccggg gtagtgaaga cactaaaact
    11941 cgaaaaaact ggtggcaccg caatcccatt gccggaagat tatctatccc ttgttcacgt
    12001 caccgacagc aatggccttt tggtctattc ggatgaacta tccggcttta ttgaattaga
    12061 gctgacaggt tcagaacgat ggccttttcg gatgctgtat ctggttaatc tccgcgacag
    12121 aaaactggac gaatggcaag tgccgcctgc aatcattggt atgttggaag aatacctgga
    12181 ggcgcttatc aatgtcagaa atgtcgcccg ccagcgtcga gcgtccatcg acgggaaatt
    12241 tgactactca gatctacctg atgaagccac gttatatgcc cgcgttcagg agattgaaga
    12301 aaaaatgtcc tcaaaccggg cgattattcc gggggctacc attttttaac gctggaggcg
    12361 cagagtgagt attttcagca gcgttggcag aacgttgacc accgcactgt catttaacac
    12421 aaaatcattc accagcaacc tcattagcga cattctggat aaagcaatat ccggcggcgg
    12481 ggtaagtggg aactacagca gcgatattgc ctacgggaaa aatattgttg ctgccgctat
    12541 gcgtattcgt tacgcccagg gatggcagtg gacggttgaa gtggacggtc tgaacggttt
    12601 cgatatgttc gtgaaggata tcacctacag cagcggaaat atcgagacag aaaacaaggt
    12661 gataggtagc gtagagttca gcaagccaac ctacgtcacc gccggaccag tcacgatgac
    12721 cgtaagggat accgaagacg gcaagatcat gaactggttc aaagagcgtc gttcccgcgt
    12781 gacaaacccg gacggaacaa taaaccttcc tcctgaatat ctaatgaaga tccggctcta
    12841 ccgggtaact caggatggcg gtaaagaact ggaagaggaa atgcgcgtat tccccactca
    12901 acttggggaa atcacccgct cacgagacca ggtgtcagag ttcttgtcat atccaatcac
    12961 tttccagaaa tacacatccg caggctcagg tgtttctgct ttggtcaacg gtgccgctgg
    13021 catggctaca agcgcgttaa agggggctgt gagtggagtg atcaaattct gaatagcttt
    13081 aattatgtgt gctggagggt tgctcatttc ccgtcagcac ccacgacctc aacgatagcg
    13141 ggtagggaag gtagcgcaat gttgagggca ttttttacat cataaatcca tcagcaatga
    13201 aggcattctt tacatatttc aataatatca atcagttaac acagtgaatg ataataaaaa
    13261 agcgcaataa gaatgcacga tacgcacgat attggttgag ggcgattttt acataatcct
    13321 tacatatcaa tacgttatat aaaaacacaa cttgctaata acgagataca tgcaattaag
    13381 atgtaaaaat tgccctcaat tgccgatcgt taagggcaag ttctacacct tgagaggggt
    13441 gtaaaaattg cccttaagac tgctgatagg taaattttat cctggtaact ttgccgcctt
    13501 ccgtcacctc tgagaagact attttcattt ttgtattttt ttcaatctct gctacagcct
    13561 tcgttaaaaa acttctctta aattcggcat accgctgata gctttttggt aacccataac
    13621 gctcccgcat ccattcgacg ccaagtatgg caaagccgct tccatcatcc ttcctgtact
    13681 ggcataagga ctcataaagc cgcatagaat aaggatttgt aaggcgagat acttcggtaa
    13741 aattcagcct tgtgaatctc ttatcaagca gagtaaaaaa cggcatgaga tatggattaa
    13801 gatgaataat gtaagtcccg cgccgtggtg aataggcatc cttaatcatc cagggataag
    13861 actcataact gtcttctgat tcggtcgatt catcagggtt atatatcgta actttcttct
    13921 cgctaagccc tgaaatggcc ttacgaatat ccttgctggc ttcagcagag ggcaaattat
    13981 acatctctgc atattcgttg accgtcaact cacaggcacc tgaaccaaga acgccatcct
    14041 tagaagcata gcgaagtctt cccaccacaa taaacaacag tcgcttttgg tcgcgagtca
    14101 ggtgataagc agcctccgtg atctcattgg cttgtgccag actacgacta ataggtgtcg
    14161 ctgactcaat caggctgacg gcttttgctg ttaattccgc tctgttcata accctgtgtg
    14221 atccttcaaa tggtgagggc aatttttaca ccactttgag ggcaattact acaccttttg
    14281 agggcaaatc ttacaccttc tgagggcaaa tcttacaccg atcgtattta attgagggca
    14341 aatcttacac cagcctcctc tacagcccgc gccactactg gctccagcga cgcctaaaaa
    14401 ggatctaaaa aggattaaaa atggaaaaag gtttataaaa atactctgtg gatatgtgag
    14461 tagcgtcact taaggtgtaa aaatcaccct caataatcgc ttctcgaccg gcaaagatct
    14521 tcattcaatc cccatcgcaa atcacacact ccctatcctc tggaaaatga acagatacct
    14581 gcttttcgga ggaataattg aacattccaa aattcccatt gccttcccgt cctgagacag
    14641 aaattcagtt ccacgcccct accgtgaagg atgcgctgaa atattctgac ctgaacccgg
    14701 cagaagatga ggcaacgact acagagtacc ttaactctat gcaggatggt gaaattaacg
    14761 acagtgctaa ctggacagtt caggaccgca gaactgctct ttggtggata tttgttaatt
    14821 cgcgccccga tgcagtaatg acctactcct atgagtgcag tcactgcggt aatacgcatc
    14881 atgccgatat taacctgagc gacttggccc aaacagtaga aatactcact gtacctcctt
    14941 acgtgaaaac caacgtacca gtaaatggag taccaactga ctggatactt aaaccattaa
    15001 ccgggaaagg cgcggaactc cttgaacgaa tgcgagcgtc acttcctgat atgaaaagtc
    15061 ccgaatacag tgctggcgta gcacggatgc gaattgctga actcgcttta tgcacggcgc
    15121 ttaaagacga tccggaggac ttcacgcagg ccgctaaccg acgctttgac atcattgaaa
    15181 gcatggcgct tgaaaccgaa tttaccccgc ttgtggctcg catacagctt atgcaaaaag
    15241 acctacgcca cggcctgaaa atgtctattg agagaggctc aagccgactg atcctgcctc
    15301 cgcaacattg caaaaacgct aaggagggtg cagatgtgac aaccacactg tacgtcccct
    15361 ttctcaatag agagtttatc ccatcaatta gatctgaatg gatggctaac cattattaac
    15421 aacctgactc tgtatggata tcagcccgtt agtgacgttg aacaattgcc acaatggcga
    15481 gcactccata tgtcaaaagc cctggaagaa aaatacaaag ctcaagccgg gaaacgttga
    15541 tcgccgctgg caaaagagag gttattttga aagagaataa agaccgtatc gcaataattg
    15601 acgctattca gggggcgagc acaaacgaac ttaacgctct tgctgagatc aaagacgcca
    15661 ttctctctgg tagagggtct ataactcaac ttggtgaaaa tcaaggtaaa gtaagtcaaa
    15721 gactatcccg tagactcata gcaagcgaac cctatgtctc tgatgatgcc aagaacaccc
    15781 aagctaaccc atatataaac cgccttccga tgaaaaaagg gaggacgcag atcaacaacg
    15841 gagaagaacg tctcattgtt gcaaccaaca catcagagcg cgttcatttt gcgtcatcac
    15901 gccagtcagc ccaaaagttg aaaggttctg tgccagtagc cgctaattct gctcctcttg
    15961 aaccacaatc cccccaaagc caaaaaggac cgcttcggga tagtaacgga cgctttgtat
    16021 cccagaaaaa caacgaagat atagccagaa aaaaagaatg gcagaaagca cataaggccg
    16081 acgccaaact acaagaaggc ttcctccgga aattaggctc cattataggg gtagatggca
    16141 atcagtcttc cagtgaagag tctttaacag atgccgcagg tgttggcgct ggtggtccgt
    16201 tgtggatggc tgctcgcggc atgtacgaca ttactaaaga gatcacaggt aaagcagaat
    16261 ctcttaaaga atgggtagaa aaggggaaag gtgaggcttc accttcgaaa gcgacatctc
    16321 cagctatcac ttatccggcg gtggttaata ctcaaaaagc aacctctgca acagcattta
    16381 ataatgctgt tgagacaaaa tcagcacaag cagtagaaga gcaaacaaaa attcttcaga
    16441 ccaatgacaa caaaataatt gatggtctgg aaaatgtctc cgacgaagta gttaagctac
    16501 gcaaatcagt atcttctggg aataagttcg gcttaagtga tctctggaga aatcgagcaa
    16561 gcagaagaaa taaaatcaat attggcgatc aggctggtaa aaataaacgg aacaaaccga
    16621 aaaggaaagg tcgcaatctg gggaaaaaag ccctttctgt tggtgaaaaa gccgctgccg
    16681 gaaccgctgc tgctggagct ggtgccggag ctgcaaagac tgttaaaaca aaggttagta
    16741 aagccaaaga catcagcacc acaagcgacg catctaaggg tattgctaaa gaaacaaaaa
    16801 acacagcaaa ggcagttaag ccagcaggtg ttgttgccga agaggccaca gttaaaacag
    16861 gtgaaactgt tgcaaagaaa aaatccgaat ccgtagccct caaaagtgct gcaaaaatag
    16921 gtgttaaatc agctgcttct acagctgcaa gagctatccc tatcatcgga tcgctggcta
    16981 tggcgggata tgacgctata gatggttaca ctgatacaga tgcgcaaaag gcagcctttg
    17041 gattaagtga cgatgatgct gtatccgaac agcaaaaaac ggcttacgca acagctaatg
    17101 tgctcgatat ggggggcttg gtgtcgggcg caacgaactt gatagggaaa ggaatttctg
    17161 cgctaggatt tgaaagagca ggtgaaaaac ttcagaattt tgataccgga gatattgctc
    17221 gtggtgttaa cggcgcggta gatgtcacca aatctgtttt tggtagcctt aaggatacat
    17281 ttttatccac tgacgaaaac acgaaacaag taaaaaaagc tgttgaagac ggcaccaaaa
    17341 aaactgtcga tgcgattcat tctctaggtg aacaattaca aggtgggcgt aatggcgagg
    17401 atggagttgg tgagcacggg tatacttctc caactgaatt tagttcacct gccaacaaca
    17461 ccattgcagc ggatctgaat attggcggca gcaacgctaa aaaccgcaat taccgaaata
    17521 ataatctcgg caatcttgtt tttgccaacc aggaaggggc gacgctggaa tcgccaaatg
    17581 caaaaggtga acaacgtttt gcgcgattta acacacctga agaagggatc agggcactgg
    17641 caaaccaggt ttccagctat tacaacggca ccagtgccgc agcgggatat cagaagttac
    17701 agacggtatc cagcatcatt tcaaaatggg caccgccaaa tgagaacaat actaaccaat
    17761 atattgataa cgtcagtaaa tatcttggtg tatcgcctaa tgaaaaaata gatgtcagca
    17821 aaccagaggt tatgacgcaa ttagttcgtg caattgcgac gaaagaaggc ggaaatccgg
    17881 cagttaataa tgagttcatt aagaacgcac ttggagcatt taacacaaac actggtcgat
    17941 gggagggaca attctctgac gaaacattgg cgcggattaa taaaattcag aaagaaaatg
    18001 gtggtcagct aattgcccgc gattcgcaat atagtgttgg tcgcaaggta aaatatgcta
    18061 atggaaaatc tccagctcaa ccagttttga atgccgttcc gacagcaaca caacagccaa
    18121 ttgaggttgc acaacatgcc caggcagtca aaaagccaca aaaaacaggt aaccaaccac
    18181 aaccgattaa tccagagaac gctgatgtta ataccggctc tccatcgagt ttgcttgagc
    18241 ggttaatcgc aataaatgaa agcggaatca gtggtgcaag taatttattt ggcggcgcgg
    18301 ctaccagcct tgctggaaca agtctggacc tggtaaagga ttttgcgttg gctacttcgt
    18361 ttggttctat atcttcgctt tccgaaaaag cgaaaggaat ggatcaggct cttactgaaa
    18421 agatatccgg cttaaccggt aaatcttttg gatttaaaaa agcttctcaa gtggctgata
    18481 tcgcaacagc catacaacaa aaacgcactg ttaatcacga tacagaaatc attgatctac
    18541 ttccgggaga tcaagtatcc ccttctactc cgaaaactcc actgcaggag caggctctag
    18601 caatcagcca ggctatggcg ggcttttcgt ctgcagcgcc agcaccgaaa accccacagc
    18661 aggagcaggc tctagcaatc agccaggcta tggagggctt ttcatctgca gcgccagcac
    18721 cgaaaacccc acagcaggag caggctctag caatcagcca ggctatggag ggcttttcat
    18781 ctgcagcgcc agcaccgaaa actccacagc aggagcaggc tctagcaatc ggccaggcta
    18841 tggagggctt ttcatctgca gcgccagcac cgaaaactcc acagcaggag caggctctag
    18901 caatcagcca ggctatggag ggagctgcgc cagtagttac ctctggaaaa cgaactatca
    18961 cggcaccaag tgatgtgcag atttatgaca acggctacaa ggtggttaca ggtgaggata
    19021 agagcttctt tggttcgcta tttgattcca cggtgtcagg gcttaaacag acaggaaccg
    19081 cagtaattcc tgccgttggc gataaccttt cacgacttgt tggggggatt gatagtacag
    19141 ggatattgaa cgatcttgta atgcagtcta ctgggcagaa tagtgcgatt gcgcgagcta
    19201 ttagcccatt aacaagaaat gttggagact ggctgaatgg ggggattcaa caaaccgcta
    19261 atagcattag gggaatagca actggtgcca ataacgccat atttggctct gcttcagctg
    19321 tgcaagaacc ttttcttgct atgccgccac aactccctac tgtaaccgat ctcgcacgaa
    19381 gcggaataag acaaccatta actactgaca ctataaataa cgatcctgct atgttgaagg
    19441 cgctggataa catctgctct atcctgaacg atctgctgaa tgtgaacaag aacaatacaa
    19501 aaggcgatcc ggataatgtt gtcaaaacat cgcagccgca gccgcgacca cgtgcgagca
    19561 caactattaa tgaccagtcg ctggatgctc tgcttgagga ttaatactga tgctttacga
    19621 aatagacgca agacttcaaa cgaatgagag cggtgtcatt attgcagaag gaagtaccgc
    19681 agcatggatg gcccgacttg acgagtggct acgaacccct gaagggagtg tttatggttt
    19741 accctcttgg gggagtccaa tggaagaatt taagcatgaa ccattcggct ctgaaacttc
    19801 gcacatagtt gaagtggcta ttgaagggag aatgatgaaa aaactacgac aagacttgcc
    19861 aggtttggat gtgcagggga ttcgttgcac atcaatttct gaagattctt tattaattag
    19921 tttttatgcg aaaggtggga gcatggatat tgttatgcag aaatcaagtg gggtgggcgc
    19981 gtgacgatta cagaattact ggacaaattt aacgccaagt taaatgaaaa cacatggtgg
    20041 tcacgatttg taaatagtca gtttgtgcag atgcacgcta tattcggatc gcagcttatt
    20101 tatattgccc gtacatttgc aagccgcggc ctcactgagg gacttatttc aacggcaacg
    20161 cgtcgctcaa gtattttagc ggttgctgaa gaccgtagct acgtagggcg atttgttagt
    20221 gcttcatacg gaacaacgtc tataaccaat aaaactgatc gggatattac gttaccagcc
    20281 ggggctgaat tgcttgccaa tgaccaaaca cctttggcaa ttattaacag tgttgtaatt
    20341 cctgctggag gaactatttc tggcgtagaa actaaacagc atgaagctgt tagcattaca
    20401 tttgatattg agaaggaaac tttatttctg acattgttgc tttccagaga gttaacaaaa
    20461 gaagtctcta gcctggatgt ttatgttatt acagatggag tagaagaaaa atggacatat
    20521 aacccattat ttagaatgtc cagagacaag agtaagcact attcattggc atataaacct
    20581 acagaacaac ttggagtcaa gtttggtgat ggttctatgg gtatgatgcc tccagcaggc
    20641 tgtcaggttc gaatcgatgt tatggctagc cttggcgact atactttggc tgaaggacaa
    20701 aagttagaac cggctggaaa tatcgctcaa tatgtggagt cactagagtt taaaactgat
    20761 tcgatcatta ccggtggtag tggtatggaa actacagaag aaactcgaaa tcgtgctcag
    20821 tattatgttg catacgatga acaagtggta tggggcggtg attatcgcca attcattcag
    20881 aatgttgttc atggaacttc atggttgaac gtttggggcg aagcgttaca agagaaaata
    20941 actgggtttg acgttcgaaa catcaacaaa attttctttt gcggacataa gccgggtgta
    21001 agccaggctc agctaaaatc agaaatactg aaagccctgg agaatgtgcc aaatgaattg
    21061 aacaagcggt tcgagtatgt agatacaaat gaacagccat ttactataaa ttttataggt
    21121 attgtacgta aaaatgttct gatagacgat gctcaaaatg ctattaaagc agcgttggaa
    21181 gataattttg gccgtgattc gtcatcattc agcttattac tacagagtga tgatgattcc
    21241 caacaatgtt atgcacaggt aaaagttaaa gatatttggc gagtaataga gtctctggat
    21301 atgtttctgt cttacgacat aacaatacaa aatatgaaag atgctgttta ctttaatgac
    21361 ttcatttatc ttaacgtaaa atcttcaacg ttcagcattt cttacccgta atgaggttag
    21421 catgaaagat aattggctta aggaacgatt aactaaagta aaacaggatt cacatctctg
    21481 gagtgcattt atagacgctt tacaggatgt ctggaatgag gaagtagaac caatattaac
    21541 aagaataagt aacagaaaat ccttcttcac tatggacagt gaggatatgg atgcccgcat
    21601 tgctgaatac gggcgttttt tcgttatcac tgaaaaagat aaggaccgta ggccaatgct
    21661 gctggcgcaa cgtttagatg aagtgcactt taaaggcact atcttgccta ttgagcagac
    21721 attctggcgt gaattcggtt gtattcctgt tagttgggaa ccgctgtatg cacctgtaaa
    21781 tatagaaaaa cacccctatg gttcatattt tgcgaccgaa atagaaatac ctacggcaca
    21841 ggctcaattc ggtgagttct tcctgacatc aagggggctg gtcgttgttg atcagaacaa
    21901 gctatatcgc tcatatggag agcaggataa agaagccgct gtgcagaaat tgttatctga
    21961 cttcgaaaca gtaatagccc ctttgttgcc attgcatatt gtttttgatg gcgtctcgtt
    22021 tcggcttagt gctgtattcc ctgaagtggc agaaatacta aattgcttgt ctacggatgt
    22081 ttcggtaatt gaaggtgttt atgttactga gagtatggct gatatgctat cccatagtga
    22141 tacctccagc caggtagaca atatttctct taacgccata ccgaaccgaa cggcagaaaa
    22201 gcaattacat cttgatgtaa ccccactcga tgcgtggcct ttggattacc accttcaacc
    22261 tgtttaataa ttcatccccc gcatttgggg gaagaacaac ggaaatcaca aactgagcat
    22321 actctccttt ggttcattgc ttatgaaggg gctatgttcg tatggctgaa aatttaaagg
    22381 taagagatgg taaccgcctg tataaggcgc aattgctttc ctactattat tcacgccgcg
    22441 ctgagtcggc gatcggcaaa ggtgcgcgtt ttgttatctc taaggcgtat tggtgcaaat
    22501 ctagtctggt aactgctaat ggtgcaggag gctggaatat agcggacatt ccactcaatt
    22561 ttaaattgag taacgctcaa cagtttgctg tgtccgatct gatattgtcc agtgtggatg
    22621 ggattatcac tatcaatgca gccttccctc aagaaaaaat gccggataac acgccttacg
    22681 actttaacac gcttgtgctt gttgatgctg aggaacaggc ttttgctgtg ctttgcaccc
    22741 agcaagacac cctctataaa ggtaaacgtt acagtattct catgacaatc gagcaagttg
    22801 agggttgatt atgggtgctg ataaaacgaa caacataatg acactatcct ctggtgtctc
    22861 acagccgttg cttgctgatg ttcaatattt cgaactctat agtagttctg ctcttaacag
    22921 aaaacttaaa aatattgttt tgccgggctt ttactgtgga tttgaaccag ttcccggcac
    22981 agggttgagc gtccgtataa cttctgaaaa ctcagaaggt aaaggggctg cttcagtaga
    23041 tgtaaataat gttcagatat ccgttcagca aatagaagat gtgactgtct cggtaaaggc
    23101 tggggctacc aacattattg tgctggaagc caattttgaa catggtgtaa aaacgacaca
    23161 ggtagatagc gcatcttctg tcagtgctgc aagaattttc gcgcgtacgg acaatgctat
    23221 tgggcagaat caaattgaat tatgtcgagt tatcgtgcct ggcggcgcaa cggctgtgac
    23281 taaagaaatg attgtgctta aataccgagt taaccgtgct gttggtgtcg aattctctaa
    23341 tgaaataagc agtacagaag aaagaaaagc ggctacacct ctggctgtca aaactcttca
    23401 tgatttggtt gatacaaaag ctccgctcaa tagtccgcat ctgtcaggta cgccgacagt
    23461 gcctacggcc tcacaaggca caaacagcac gcagatcgca aatacagcct ttgttaaggc
    23521 agctataact gcacttatca acggtgcgcc tggcacactg gatacgctga aagaaatagc
    23581 ggctgcgatc aataacgacc cgaattacag cacaactatc aacaatgcct tggctctcaa
    23641 agcgcctttg gcaagccctg cattaacggg tgtccctact gcgcctacgg ctgcacaggg
    23701 cacaaacaat acgcagatcg ctacgactgc ttacgtacgg gctgctatct ctgcattggt
    23761 cggctcatca cctgaagctc ttgataccct gaatgagctt gcagcagcac tgggcaatga
    23821 cccgaacttt gcgacaacaa tgacaaatgc gctggcaggg aaacagccac ttgatgcaac
    23881 tttaaccgcg cttgctggtc ttgcgacagg cgcaaacaaa ctgccttatt tcactggtaa
    23941 ggatacggta gcgcagactg atttaacgtc agtcggtcga gatattctgg ctaaaacaag
    24001 cacactggcc gttatccaat accttggttt aagagaactc ggtaccagcg gtgaaaagat
    24061 ccccctgttg agcacggcta acacatggag tgcgcgccag actttcaacg gcgggatcac
    24121 cggggcgctg acagggaacg ccgacaccgc gacgaaactt gcgacggcaa gaaacatcaa
    24181 cggtgttaaa tttgatggtt ctgtggacat ttcgatacca acaattacgt ccagagggcg
    24241 tgttactgcg ctcaccggta caacacaagg tgctgctact ggattgcaaa tgtatgaggc
    24301 gtacaacaac ggctatcctt ctgcttacgg gaatgtgctt catcttaagg gcgcaacggc
    24361 agttggtgaa ggtgagttgt tcataggttg gagcggtaca agtggcgctc atgcacctgt
    24421 acatgtacgt tcgcgacgag atactgatac agccagttgg tcagagtggg cgcaggtcta
    24481 cacatcgaag gattccatcc ctggtgtaaa taccacgggc aaccagaaca caaccggaaa
    24541 cgcggcgtct gcaacgaaat tacaaacggc aagaactatt ggtggtgttt catttaatgg
    24601 caccgcaaac atcgacctgc cgggcgtgaa taaaacaggc aatcagagca ctaccggcaa
    24661 tgcagctaca gccacaaaac tgcaaacagc acgcaccatt aatggggttt catttgacgg
    24721 aacggctaat atttctctgt caccggcaaa tattggttgc ccggcatctc ctactggttg
    24781 gcttgtaaca ggtgataatg gagcttcaat aacaacagag caattagtga ctctgctacg
    24841 agataatggt gcttttaatg caaaggcatg gatagcgcgt tgtgcctggg cgtatgcaaa
    24901 tagtgcatcc ataccagaca gcgaaacagg atgcggcatt attccactgg ctggcgcagt
    24961 tattgaggta tttaataacg gcaatagctc aaacaattat acgattcgta taacaacggc
    25021 aacaacgaca ggtgtctctg gcgcactcac taatgcggag tttgtctatg tgtttaacgg
    25081 gacgagctat tcaccaggat ggcgcagagc gtataacact aaaaacaagc ctactgcggc
    25141 tgatgtcgga gctttaccac tatctggagg agcgttaact ggcggattaa ctgctgctgg
    25201 cgagattatt tccaaatcgg cgaatggtct tcgcattgcc tatggcaact atggattctt
    25261 tatccgtaat gacggcggca acacatattt catgctaaca gcatcaggag atacgttagg
    25321 ttcatggaac agtttgcgac cgattacaat taataatacc agcggtgttg tgtctattgg
    25381 taatggacta
//
LOCUS       DAJVRP010000058        24983 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVRP010000058 DAJVRP010000000
VERSION     DAJVRP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.058"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..479
                     /gene="tnpA"
                     /locus_tag="OQN12_002403"
                     /pseudo
     CDS             1..479
                     /gene="tnpA"
                     /locus_tag="OQN12_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            807..1889
                     /locus_tag="OQN12_002404"
     CDS             807..1889
                     /locus_tag="OQN12_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCS1654697.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(2378..3176)
                     /locus_tag="OQN12_002405"
                     /pseudo
     CDS             complement(2378..3176)
                     /locus_tag="OQN12_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3186..3737)
                     /gene="ybcL"
                     /locus_tag="OQN12_002406"
     CDS             complement(3186..3737)
                     /gene="ybcL"
                     /locus_tag="OQN12_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCS1654698.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3906..4238)
                     /gene="emrE"
                     /locus_tag="OQN12_002407"
     CDS             complement(3906..4238)
                     /gene="emrE"
                     /locus_tag="OQN12_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCS1654699.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(4583..4897)
                     /gene="fliE"
                     /locus_tag="OQN12_002408"
     CDS             complement(4583..4897)
                     /gene="fliE"
                     /locus_tag="OQN12_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1654700.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5111..6776
                     /gene="fliF"
                     /locus_tag="OQN12_002409"
                     /pseudo
     CDS             5111..6776
                     /gene="fliF"
                     /locus_tag="OQN12_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            6769..7764
                     /gene="fliG"
                     /locus_tag="OQN12_002410"
     CDS             6769..7764
                     /gene="fliG"
                     /locus_tag="OQN12_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS1654701.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7757..8443
                     /gene="fliH"
                     /locus_tag="OQN12_002411"
     CDS             7757..8443
                     /gene="fliH"
                     /locus_tag="OQN12_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1654702.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            8443..9816
                     /gene="fliI"
                     /locus_tag="OQN12_002412"
     CDS             8443..9816
                     /gene="fliI"
                     /locus_tag="OQN12_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCS1654703.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9838..10179
                     /gene="fliJ"
                     /locus_tag="OQN12_002413"
                     /pseudo
     CDS             9838..10179
                     /gene="fliJ"
                     /locus_tag="OQN12_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10176..11303
                     /gene="fliK"
                     /locus_tag="OQN12_002414"
     CDS             10176..11303
                     /gene="fliK"
                     /locus_tag="OQN12_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1654704.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            11408..11872
                     /gene="fliL"
                     /locus_tag="OQN12_002415"
     CDS             11408..11872
                     /gene="fliL"
                     /locus_tag="OQN12_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1654705.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11877..12881
                     /gene="fliM"
                     /locus_tag="OQN12_002416"
     CDS             11877..12881
                     /gene="fliM"
                     /locus_tag="OQN12_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS1654706.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12878..13291
                     /gene="fliN"
                     /locus_tag="OQN12_002417"
     CDS             12878..13291
                     /gene="fliN"
                     /locus_tag="OQN12_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1654707.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            13294..13659
                     /gene="fliO"
                     /locus_tag="OQN12_002418"
     CDS             13294..13659
                     /gene="fliO"
                     /locus_tag="OQN12_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1654708.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            13659..14396
                     /gene="fliP"
                     /locus_tag="OQN12_002419"
     CDS             13659..14396
                     /gene="fliP"
                     /locus_tag="OQN12_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCS1654709.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            14406..14675
                     /gene="fliQ"
                     /locus_tag="OQN12_002420"
     CDS             14406..14675
                     /gene="fliQ"
                     /locus_tag="OQN12_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1654710.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14684..15469
                     /gene="fliR"
                     /locus_tag="OQN12_002421"
     CDS             14684..15469
                     /gene="fliR"
                     /locus_tag="OQN12_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS1654711.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            15754..16377
                     /gene="rcsA"
                     /locus_tag="OQN12_002422"
     CDS             15754..16377
                     /gene="rcsA"
                     /locus_tag="OQN12_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1654712.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(16421..16609)
                     /gene="dsrB"
                     /locus_tag="OQN12_002423"
     CDS             complement(16421..16609)
                     /gene="dsrB"
                     /locus_tag="OQN12_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1654713.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16772..16999
                     /gene="yodD"
                     /locus_tag="OQN12_002424"
     CDS             16772..16999
                     /gene="yodD"
                     /locus_tag="OQN12_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCS1654714.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            17297..18112
                     /locus_tag="OQN12_002425"
                     /pseudo
     CDS             17297..18112
                     /locus_tag="OQN12_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18109..19803)
                     /gene="yedQ"
                     /locus_tag="OQN12_002426"
                     /pseudo
     CDS             complement(18109..19803)
                     /gene="yedQ"
                     /locus_tag="OQN12_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(19974..20156)
                     /locus_tag="OQN12_002427"
     CDS             complement(19974..20156)
                     /locus_tag="OQN12_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1654715.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20235..21152)
                     /locus_tag="OQN12_002428"
     CDS             complement(20235..21152)
                     /locus_tag="OQN12_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1654716.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            21325..22245
                     /gene="yedA"
                     /locus_tag="OQN12_002429"
     CDS             21325..22245
                     /gene="yedA"
                     /locus_tag="OQN12_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCS1654717.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22234..22704)
                     /gene="vsr"
                     /locus_tag="OQN12_002430"
     CDS             complement(22234..22704)
                     /gene="vsr"
                     /locus_tag="OQN12_002430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1654718.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22685..24103)
                     /gene="dcm"
                     /locus_tag="OQN12_002431"
     CDS             complement(22685..24103)
                     /gene="dcm"
                     /locus_tag="OQN12_002431"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1654719.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24170..24710)
                     /locus_tag="OQN12_002432"
                     /pseudo
     CDS             complement(24170..24710)
                     /locus_tag="OQN12_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(24750..>24983)
                     /locus_tag="OQN12_002433"
     CDS             complement(24750..>24983)
                     /locus_tag="OQN12_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654720.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6312 a         6412 c         6156 g         6103 t
ORIGIN      
        1 agatcacgtc catctgttaa ttaactatcc ccccaaactg gcgatatcca gtctggtaaa
       61 caaacagcct caaaggcgta tcgggtaggt tactgcgacg ggatcgacca gatattgctg
      121 ccaggtatta ccgcaaagat gttttgtggc gtcccggcta ttttgccagt agctgcggag
      181 gtgcgccaat atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcagatga
      241 aaaaacgagc cttatatccc cgccctgaag gacgggcttt tacggtgcac tggataagcc
      301 tggagattat ctacacctgc atggacatca ttgtactatg ccccgtacat gccccatcac
      361 atcctcaaca tcaaagtatt agagccttgc aaaccattat gcgtctcagt ttgtctcatc
      421 tatcttacgc aaaaaaaata gaactaatat caagcggcgc tatattgcgt attaaataaa
      481 accacctcaa ttacattaat aaatctaaat aaatatcatg caaataattt ccatggttat
      541 ttttaggcta tttaaattaa aaaaataaat tatgagtcaa tcataaataa caacctttaa
      601 tataatttga ctaccaaatt tatattcgtt cacaaaatag tcatgcattg tgtgcaatag
      661 aaacagttca gataaaaata gggatctact tcacaattta aatgagaaac taaaacttac
      721 atcttgaaat aatctcattg attggatgaa tatttgtagt gcagtgacat catttttaaa
      781 taatagttca aataaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      841 ctgcatcagt actgatggcg atgtctgctc aggcagcaga gatttacaat aaagacagta
      901 acaagctgga tctgtacgga aaagttaatg ctaagcacta cttctcttct aacgatgcag
      961 atgatggcga cacaacttat gttcgtctgg gcttcaaagg cgaaactcaa atcaacgatc
     1021 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgtgct gaatctcaag
     1081 gttcttccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
     1141 ccatcgatta tggccgtaac tacggtgtag catacgatat cggtgcatgg accgacgttc
     1201 tgccagaatt cggtggcgat acctggaccc aaacagatgt gttcatgact ggtcgcacca
     1261 caggtgttgc aacctatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
     1321 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaac ggtgatggtt
     1381 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1441 ctgaccgcac taatgatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1501 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1561 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1621 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1681 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg attgaatata
     1741 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1801 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1861 ttgctgtagg attggtttac cagttctaat ctgattactc aaagatatgt tgtgggaggc
     1921 tttgcctccc caacatataa gtggcgctct caagccactt tccttagaag cactatcttg
     1981 cttctgacta tataaattac cctttatttt ctgggacgtt tcaacgcccc atttttaata
     2041 atactcagtg aacaattaac gtatcaatta gatttaataa caacgatatc cgtacctaac
     2101 ctgatatcta atgtcataag catcccttta attatcccct caacctcttg taaatttccc
     2161 actcttgagc tccacatagc ccaaacgccg gatgcaaaaa aaatcacacg tgcatctctg
     2221 aaattgtgat ggaggaagtg agtatcaatt atatgtgctt ataaacgttc ccgattggca
     2281 cagaagtcac cggattgttc aggctccaat aggttgagta tgtcgtattt attttccaaa
     2341 ataaaaatcg atgctgttgc tccttaataa aatactttta ctctctggac gcttttctta
     2401 gctctcttgg tgttatatta aaacgattat ggaacctttc agtaaaacga gaaggacact
     2461 tgtaaccatt ttccctggca atctcgctga tgggttttac tgtcgtttgt atagcaggac
     2521 aacgcattat ttaacctcac gtcgtccaat atactttgaa aacttattcc ctcgcttgcc
     2581 agacggcgat gcaatgtgga aacagaaatg tagagatatc tcgctacctt gtttgctgtc
     2641 cattttgttc taggttcgga tagcattagg ttataacaac gacttattaa tgattgttta
     2701 ctatctgata aaagtaaaca attaacatga tgtgctccta atgaaagtag aacgcccatt
     2761 gctaagtgtt cctgaatttg cgttgagaag cctcgggaaa tagatgtttt tagttgctcc
     2821 caacaatata ttaactcggg gttctgaggt aagcagaaac ctgtcgtgtt acgaatttga
     2881 tcagttatcg cataaaattt ttgaaacttt tcaattagat caatgggata ataaagcatt
     2941 tctgcaagat aaagacctgc ttctggataa ttcacaatat aaaattcata tccacaggga
     3001 aataatatta tctgattatt atcaacagtt agactatgcg tctcccaatt gataactttt
     3061 tttccctgac ggatacgaca taaagctggc ataagaggct taaccctgtg aatctcatga
     3121 tgtttatgca tccgtatttc ttcaatcttt aggttagact tatctcttgc cagcatactc
     3181 ttaccctatt ttatctcata aactggtgtt atctcagcgg ttgcaatttt attggcatta
     3241 agcatataac caactaacgc tccgctggaa ttagaatcca caggaatctt atcagttttt
     3301 agagcccata ctttaaactg gtaatgatgg ggtttatctc ctttgggagg acatgctcca
     3361 ccaaatcctg cataaccaaa atcatttcgg ccttgaacag caccagtcgg aagttttgta
     3421 ccatcacgtc ttcctgcatc agtgggcaaa tatgttacag ttgctggaat attagcaaca
     3481 gtccagtgcc accatccact gcctgtagga gcatctggat catacacagt tacggcaaag
     3541 cttttggtgc cttcaggagc accagaccag gttaatgagg gcgatgtatt accaccttca
     3601 cacccaaatc cagaaaatac atgagacgtt gtaagttgct ctcctgtttt tatttcatta
     3661 ctagtgacct gaaatgctgc ggcctgcgca gaaaatgtta tgaatgccaa tacacttgaa
     3721 acgataagtt ttttcataaa aacctcattg ttgtgaccta tcgttatttt atttgatgtg
     3781 ccttgatctc attatgaata aaggcgcaat gatcgcgcaa atgcaatcat aattataaag
     3841 gtgccccgaa cattagccat aatatgcaca aatcagattc ctgttatctc aaggagaatt
     3901 attttttaat gcggtgcact tcgtgacaac aagttaatga ctaacacacc ggcacaaatc
     3961 aacatcatgc ctatgatagc tggcaggtcc agtcgttgac caaaaaatcc ccaggaaagc
     4021 aaactaatca gaacgatacc gactcctgac cagatagcat aagcaatccc tgtaggtata
     4081 taagccagcg tctgagctaa taaccagaat gatgcacaat agcaaataat tgtgccaaca
     4141 gagggccata accgtgtaaa cccctctgaa aacttcatta aggttgtacc aatgacctct
     4201 gcaagtattg cgccaccaag ataaatataa gggttcatag catattcttt cctgttcata
     4261 ctggagagaa ttgtactaca gtttgaacac aactcaccgt tttcatcctg gtgtatgtgt
     4321 atccaagggg ctctggcgtg gcaagagata aaggcaggaa caataatgat cagaaggaac
     4381 cccaggctaa cgaatgagtt cactcttcct gggagcaaag cgaagacaaa acatgttatc
     4441 gcgtttacgg atcgtggatg gcagaaaaca accaggatca ggtatccatt ttaaaccaga
     4501 aattgagtga gtttgcccca tccatgcccc acgctgtggg atcggatata attaaacaag
     4561 catagtcaac aggttacaca acctacacct gcatggacat cacttcctga tacgccgcca
     4621 ccagcttatt acgcacctga atccccattt gcatagaaac tgaggctttt tgcatatcgg
     4681 tcatcacatc gtttagcgcc acgccgggtt caccgagggt gaatttttct gcctgcgtgc
     4741 gggcgactgt ttgtgtatcg cttatgcgat cgagcgcggc gtgcagctgc ccggcaaaac
     4801 taatggtcgg ttgcggcagt gattcctgcg cacacgcact catcgccgta gcctgtaact
     4861 ggctgataac cccttcaatc ccctgtatcg ctgacatttt cgtctcccgg ataatttctg
     4921 gtaacaaagc ctaccagtaa gtcaataaga caaaggcgct aaatagcaac aaaaaaactg
     4981 gtttattggc ggatagaaaa aacgaaagca caaataatgg gagcgtcaat ttttcgagtt
     5041 tgctgacccg agagtgagtc ttgttccact ttgccaataa cgccgtccat aatcagccac
     5101 gaggtgcgcg atgaatgcga ctgcagccca gacaaaatct cttgagtggc ttaatcgcct
     5161 gcgtgcgaat ccgaaaattc cattgattgt taccggttcc gcggcagtgg cggtcatggt
     5221 cgcactgatc ctgtgggcga aagcccccga ctaccgcaca ttattcagca atctttccga
     5281 tcaggatggt ggcgcaattg tcagccaact gacgcaaatg aatattcctt accgcttcag
     5341 cgaagccagc ggcgctattg aggttccggc agataaagtt cacgaactgc gcctgcgcct
     5401 ggcgcaacaa ggtttgccca aaggcggcgc ggtcggtttc gaactgctcg atcaggaaaa
     5461 gtttggtatc agccagttca gcgaacaggt gaattatcag cgggcgctgg aaggcgagct
     5521 ttctcgtacc atcgaaacta tcggcccggt aaaaggggcg cgcgtacatc tggcaatgcc
     5581 gaaaccgtct ttattcgtcc gtgaacaaaa atccccttct gcatcggtga cggtaaatct
     5641 gttacccggc cgcgcactcg atgaagggca aattagcgct attgtacatc tggtttccag
     5701 cgcggttgct ggtctgccgc ctggaaacgt cacgctggtg gatcagggcg ggcatctgtt
     5761 aacccagtcc aataccagcg ggcgcgatct taatgacgct cagttgaaat atgccagcga
     5821 tgtcgaaggc cgtattcagc agcgtattga agcgatcctg tcacctattg ttggtaacgg
     5881 taatattcac gcctaggtca cggcgcaact ggacttcgcc agtaaagaac aaacggaaga
     5941 acagtatcgc cctaacggtg atgaatctca tgcggcgctt cgttcacgcc agcttaatga
     6001 gagcgagcaa agcggttccg gttatccggg cggcgtaccg ggggcgttgt cgaatcaacc
     6061 ggcacctgcg aataacgcgc caatcagcac gcctccgaca aatcaaaata accgccagca
     6121 gcaggcgagc accaccagca atagtgggcc gcgtagcaca cagcggaatg aaaccagtaa
     6181 ctacgaagtc gatcgcacca ttcgtcatac caaaatgaac gtgggcgatg tgcaacgtct
     6241 gtcagtcgcg gtagtggtga attacaaaac cttgccagac ggtaaaccat tgcctctcag
     6301 caacgaacag atgaagcaaa ttgaagctct gacccgcgag gcgatgggct tttctgaaaa
     6361 acgcggtgat tcgctcaatg ttgttaactc gccgttcaat agcagtgacg aaagcggcgg
     6421 agcactgccg ttttggcaac agcaagtgtt tatcgatcag ttgctggctg ccggtcgctg
     6481 gttgctggtt gctggtactg ctggtggcgt ggttgctgtg gcggaaagcg gtacgcccgc
     6541 agctaacacg tcgcgccgag gcggtgaaga ctgtgcagca acaggcgcag gcccacgagg
     6601 aagtggaaga tgcggtggaa gtccgtctga gcaaagacga acaactccaa caacggcgcg
     6661 ctaaccaacg actgggggca gaagtcatga gccagcgtat ccgtgaaatg tctgataacg
     6721 atccgcgcgt ggtggcgctg gtcattcgcc agtggataaa taacgatcat gagtaacctg
     6781 acaggcaccg ataaaagcgt catcctgctg atgaccattg gcgaagaccg ggcggcagag
     6841 gtgttcaagc acctctccca gcgcgaagtg caaaccctga gcgctgcaat ggcgaacgtc
     6901 acgcagatct ccaacaagca gctaaccgat gtgctggcgg agtttgagca agaagctgaa
     6961 cagtttgctg cactgaatat caacgccaac gattatctgc gttcggtatt ggtcaaagct
     7021 ctgggtgaag aacgtgccgc cagcctgctg gaagatattc tcgaaacccg cgataccgcc
     7081 agcggtattg aaacgctcaa ctttatggag ccgcagagcg ccgccgatct gattcgcgat
     7141 gagcatccgc aaattatcgc caccattctg gtgcatctga agcgcgccca ggccgccgat
     7201 attctggcgt tgttcgatga acgtctgcgc cacgacgtga tgttgcgtat cgccaccttt
     7261 ggcggcgtgc agccagccgc gctggccgaa ctgaccgaag tactgaatgg cttgctcgac
     7321 ggtcagaatc tcaagcgcag caaaatgggc ggcgtgagaa cggcagccga aattatcaac
     7381 ctgatgaaaa cgcagcagga agaagccgtt attaccgccg tgcgtgaatt cgacggcgag
     7441 ctggcgcaga aaatcatcga cgagatgttc ctgttcgaga atctggtgga tgtcgatgat
     7501 cgcagcattc agcgtctgtt gcaggaagtg gattccgaat cgctgctgat cgcgctgaaa
     7561 ggggccgagc agccactgcg cgagaaattc ctgcgcaata tgtcgcagcg tgccgccgat
     7621 attctgcgcg acgatctcgc caaccgtggt ccggtgcgtc tgtcgcaggt cgaaaacgaa
     7681 cagaaagcga ttctgctgat tgtgcgccgt cttgccgaaa ctggcgagat ggtgattggc
     7741 agcggcgagg atacctatgt ctgataatct gccgtggaaa acctggacgc cggacgatct
     7801 cgcgccacca ccggcagagt ttgtgccaat ggtcgagtcg gaagaaacca ttattgaaga
     7861 ggccgaaccc agccttgagc agcaactggc gcaactgcaa atgcaggccc acgagcaggg
     7921 ttatcaggcg gggattgccg aaggtcgcca gcaaggtcac gaacagggct atcaggaagg
     7981 actggcccag gggctggaac aaggtctggc agaggcgaag gctcaacaag cgccaattca
     8041 tgcccggatg cagcaactgg tcagcgaatt tcaaactacc cttgatgcgc ttgatagtgt
     8101 gatagcttcg cgcctgatgc agatggcgct ggaggcagca cgtcaggtga tcggtcagac
     8161 accaacggtg gataactcgg cactgatcaa acagatccaa cagttgttgc agcaagaacc
     8221 gttattcagc gggaaaccac agctgcgcgt acacccggat gatctgcaac gtgtggatga
     8281 tatgctcggc gcaactttaa gtttgcatgg ctggcgcttg cggggcgatc ccaccctcca
     8341 ccctggcgga tgtaaagtct ccgccgatga aggcgatctc gacgccagtg tcgccactcg
     8401 ctggcaagaa ctctgccgtc tggcagcacc aggagtggtg taatgaccac gcgcctgact
     8461 cgctggctaa ccacgctgga taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt
     8521 cgctacgggc gattaacccg cgctaccggg ctggtgctgg aagccaccgg attacaattg
     8581 ccgctcggcg caacctgtgt cattgagcgc cagaacggca gcgaaacgca cgaagtagaa
     8641 agcgaagtcg ttggctttaa cggtcaacgg ctgtttttaa tgccgctgga ggaagtggaa
     8701 ggtgtcctgc ccggcgcgcg tgtatatgcc aaaaacattt cggcagaagg gctgcaaagc
     8761 ggcaagcagt tgccgctcgg tccggcgtta ttaggtcgtg ttctggacgg tagcggtaaa
     8821 ccgctcgatg gcctgccctt ccccgatacg acggaaaccg gtgcgctgat taccccgcca
     8881 tttaacccgt tgcaacgtac accgattgaa catgtgctgg acaccggcgt gcgcccaatc
     8941 aatgccctgc ttaccgtcgg acgcgggcag cgtatggggc tgtttgccgg gtcgggcgtt
     9001 ggtaaaagtg tgctgctggg gatgatggca cgctacaccc gcgccgatgt cattgtcgtg
     9061 ggtttgattg gtgaacgtgg gcgcgaagta aaagatttta ttgagaatat cctcggtgcc
     9121 gaaggacgtg cacgctcagt ggtgattgcc gctccggcgg atgtttctcc gctcctgcga
     9181 atgcaaggtg ccgcctatgc cacgcgcatt gccgaagatt ttcgcgatcg tggtcagcat
     9241 gtgttgctga ttatggactc cctcacccgc tacgcgatgg cccagcgtga aattgcgctg
     9301 gcgattggcg aaccacctgc cactaaaggt tatccaccgt cggtgtttgc caaattaccg
     9361 gcactggtcg agcgtgccgg taatggcatt agcggcggcg gctcgattac cgcgttttat
     9421 accgtgctca ccgaaggcga tgaccagcag gaccccattg ctgactccgc gcgggcaatc
     9481 ctcgacggtc acattgtgct gtctcgccga ctggcggaag ccgggcacta tccggctatc
     9541 gatattgaag cgtcgatcag tcgcgcaatg acggcgttga tcagtgagca acattacgcg
     9601 cgagtgcgca ccttcaaaca gctgttgtcg agttttcagc gtaaccgcga tctggttagc
     9661 gtcggcgcgt atgccaaagg cagcgatccg atgctcgata aagccatcgc cctgtggccg
     9721 cagctggagg gctatttgca acaaggcatt tttgaacgcg cggactggga agcgtctctc
     9781 caggggctgg agcgtatttt cccgacagtg tcataaccca ggagataacg gcagatggcg
     9841 gaagaacagc tcaaaatgtt gatcgattat cagaatgaat atcgcaataa cctcaatagc
     9901 gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg
     9961 ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca gaaagttgac
    10021 attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca gacactgcag
    10081 gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat
    10141 gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg cccttgatta
    10201 ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct gcacaagatt
    10261 ttctcacgtt gttgagcgaa gcattagcag gcgagacaac aactgacaaa gcggcccccc
    10321 agttgctggt agcaacagat aagcccacga caaaaggcga gccgctggtc agcgatattc
    10381 ttgccgatgc gcaacaagcg gatttactga tccctgtgga tgaaacactt cctgtcatca
    10441 acgacgaaca atccacatca acaccgttaa ccaccgctca gacgatgacg ttggctgcgg
    10501 tggctgacaa aaatacgaca aaagacgaaa aagcggatga tcttaatgaa gacgtcaccg
    10561 caagtctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc aaagtgactg
    10621 atgcaccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa ctgacttctg
    10681 cgcaactcac tacagcacag cctgatgacg cccccggcac accagctcag ccattaacac
    10741 cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacgccttcg ccggtgaccg
    10801 ctgacgccag cccgctaatc actccacacc agacacagcc actgcccacc gtcgccgcgc
    10861 ctgttttgag tgcaccgctg ggttctcacg aatggcaaca atcattgagc cagcatattt
    10921 cgctgttcac ccgccagggg caacaaagtg cagagttgcg tctgcaccca caggatttag
    10981 gtgaagtgca aatttccctc aaagtggatg ataaccaggc gcagatccag atgatttcac
    11041 cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt acgcagctgg
    11101 ccgaaagtgg cattcagtta gggcaaagca acattagtgg cgaaagcttt agtggtcagc
    11161 agcaggccgc ttcccagcaa cagcaaagcc aacgcacagc aaaccatgaa cctctggcgg
    11221 gggaagacga cgatacgctt ccggttcccg tctctttaca agggcgtgta acaggtaaca
    11281 gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg
    11341 ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc
    11401 gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat
    11461 tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca
    11521 tcaggttgcc gctgacgaca aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc
    11581 gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata tcggcataac
    11641 cctgcgactg aaagatgaag caacccgctc gcggctgagt gagtatttgc cggaagtccg
    11701 tagtcgcttg ctgttactgt tttcacgtca ggatgctgcc gtactggcga cagaagaagg
    11761 caagaaaaac ctgattgccg agattaaaac cacactttcc accccgcttg ttgccgggca
    11821 accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat aacgacatgg
    11881 gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac agcgaagtca
    11941 aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata
    12001 cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg
    12061 cccgccattt tcgtatgggg ctgttcaacc tgctgcgccg tagcccggac attaccgtcg
    12121 gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc
    12181 tgaacctaat ccacctgaaa ccgctacgcg gcacagggct ggtagtgttc tcgccgagtc
    12241 tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg accaaagtgg
    12301 aaggtcgcga gtttacccat actgaacagc gcgtcatcaa ccgtatgttg aagctggcgc
    12361 tggaaggcta tagcgacgcc tggaaagcga ttaatccgct ggaagttgag tacgtgcgtt
    12421 cggaaatgca ggtggaattt accaatatca ccacctcgcc gaacgacatt gtggttaaca
    12481 cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgc ctgccattca
    12541 gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg
    12601 aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctggaactgg
    12661 tggccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg
    12721 acgtcctgcc gatagaaaaa cccgatcgca tcatcgccca tgttgacggc gtcccggttc
    12781 tgaccagtca gtacggtact ctcaacggtc agtatgcgtt acggatagaa catttgatta
    12841 acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga ataatccggc
    12901 cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg agcaaaaatc
    12961 aaccagcgaa aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag
    13021 cggaacgttg caggatatcg acctgattat ggatattccg gtcaagctga ccgtcgagct
    13081 gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt cagtcgtggc
    13141 gctggacggt ctggcgggcg aaccgctgga tattctgatc aacggttatt taatcgccca
    13201 gggcgaagtg gtggttgttg ccgataaata tggcgtgcgg atcaccgata tcattacccc
    13261 gtccgagcgg atgcgccgcc tgagccgtta gtgatgaata accatgctac tgtgcaatct
    13321 tccgcgccgg tttctgctga gccactgctg caggtgagcg gcgcactcat cgccattatt
    13381 gccctgatcc tcgctgctgc ctggctggta aaacggctgg gatttgcccc taaacgcact
    13441 ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggtcgtg
    13501 gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg
    13561 ctgcataaac ttcccccttc agcaccaacg gaagagatac cgcagaccga ttttcagtcg
    13621 gtcatgaaaa atttgcttaa gcgtggcggg agatcctgat gcgtcgtttg ttttctgtcg
    13681 caccggtcct tctctggctg attacgcccc tcgccttcgc gcaactgccg ggtatcacca
    13741 gccagccgct acctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca
    13801 tcacctcgtt gacgttcatt ccagcgattt tactgatgat gaccagtttc acccgcatca
    13861 ttattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg
    13921 tattgctggg gctggcactg tttttgacct tttttattat gtcgccagtg atcaacaaaa
    13981 tttatgtcga tgcgtaccag ccattcagcg aagagaaaat ctcaatgcag gaggcgctgg
    14041 aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgcgag gcagatttag
    14101 ggttgtttgc cagactggcg aataccggcc cgttgcaggg gcctgaagct gtgccgatgc
    14161 gcattttgct cccggcctac gtgaccagcg agttgaaaac cgcatttcag ataggcttca
    14221 cgattttcat cccttttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg
    14281 ggatgatgat ggttcccccc gccaccattg ctctgccctt taaactgatg ctgtttgtac
    14341 tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag
    14401 gcaaaatgac acctgaatcg gtcatgatga tgggaactga agcgatgaaa gtcgcgctgg
    14461 cactggcagc cccgctgttg ctggtagcgc tggtcacggg ccttatcatc agtattttgc
    14521 aggcagccac gcagattaac gaaatgacgc tgtcgtttat tccgaaaatc atcgccgtat
    14581 ttatcgccat cattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca
    14641 ccttgttcac taacctgccg tatatcatcg ggtagcccgt acaatgatgc aggagacaag
    14701 cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg tgctggcgct
    14761 catttctacc gcgccaattc tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct
    14821 ggcaatgatg atcacgttcg ccattgcccc atcattacct gccaacgatg ttcctgtttt
    14881 ttcgttcttt gctctgtggc tggccgtaca gcagatcctg atcggcattg cgcttggttt
    14941 taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat
    15001 ggggctgtca tttgcgacat ttgtcgatcc tggcagccat ctcaatatgc ccgttttagc
    15061 gcgcatcatg gatatgctgg cattactgct gttcctgaca tttaacggtc atttatggtt
    15121 gatttcactg ctggtcgata cctttcacac cctgccgatt ggtagcgaac cgttgaacag
    15181 caatgcgttt ctggcaccca ccaaagcagg gagtttgatt ttccttaacg ggctgatgct
    15241 ggcgttaccg ctcattactc tgttgctgac cctgaatctg gcattaggtt tactgaatcg
    15301 tatggccccg caattatcca tttttgttat tggatttcca ttaactctga ctgtcggcat
    15361 ctctttaatg gcggcattaa tgccgttaat tgcgcctttt tgcgaacatt tattcagtga
    15421 aatttttaat ttgctggctg atattattag tgaattacca ttaatataat tccgtaacgt
    15481 ttatcatgct accctaagga ttatccgaaa aataatatct gcgaacatct tccaggatac
    15541 tcctgtagcg aaatatttgt tttaagctca ctcacatatc gcaacattta ctttaagaca
    15601 attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa aattgcgaga
    15661 gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt caggtaaggg
    15721 gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat ggatttatgt
    15781 agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa aaaaagagaa
    15841 atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca gcgtccttca
    15901 gtggtgttta ttaatgagga ctgtttcatc cacgatgctt ctaacagtca gcgcatcaag
    15961 cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat tgccaatgtt
    16021 cattttgatg aatatctatt ggtcagaaaa aatttattga tcagttctaa atcgattaaa
    16081 ccggaatctc tcgacgatat ccttggcaat attctgaaaa aagagacaac gataacctcg
    16141 tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg
    16201 atggcaggtc agggaaccat tcaaatctcc gaccaaatga atatcaaagc caagaccgtt
    16261 tcatcgcata aaggtaatat taaacgtaag atcaaaacgc ataataaaca ggttatctac
    16321 catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat gcgctaacac
    16381 attctgactg gtgatttccc accagtcagg ctgaataaga ttactctgct ttctcaacaa
    16441 agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg tagtcttcca
    16501 gcgcaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc actccaggac
    16561 gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc gttttcctcc
    16621 tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagcgccaag cgcgcgcgag
    16681 tgctgatttt ctcgacggtc tatacttaag agatgctagg cggacttaac gactggcggc
    16741 aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagtgt acagcgatac
    16801 cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca atgaaattag
    16861 cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg atggacgtga
    16921 atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg acttcagcgt
    16981 ctctgaagtg aatcgttgaa ttcacaataa aaaaatcccg accctgaggg tgtcgggatg
    17041 aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc
    17101 atcaccttat ctgcaatttt tttcgctgac aagaaaatat tcgcgctatg aatgattaac
    17161 gctacttatg aatagaaatg tgacgcgcgt caccccttgg tggctctggt gtcaacggga
    17221 tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat cctggtaagg
    17281 ggagtactgc gcgaccatgt tttcaattca acaaccacta ctggttttta gcgatcttga
    17341 tggcaccctg ctggacagtc atagttatga ctggcaaccg gcagccccct ggctcagccg
    17401 tttacacgaa gccaatattc ccgtcattct ctgtagcagt aaaactttag cggaaatgct
    17461 gtacttgcaa aaaatgttgg ggctacaagg cttaccgctg attgcagaga atggcgcagt
    17521 gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca tctcaggtat
    17581 tagccatggc gaaatctgcc aggttttaaa tacgctacgc gagaaagaac attttaaatt
    17641 cacgactttt gatgatgtcg acgatgcaac cattgccgaa tggacgggat taagccgtag
    17701 ctaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc gcgacagtga
    17761 cgagcgtatg gcacaattta tcgctcgtct gaacgaactg ggcttgcagt ttatgcaggg
    17821 tgcgcgcttc tggcacgtac tggatgcctc tgccggaaaa gatcaggctg ccaattggat
    17881 tatcgcgacc tatcaacaat tgtcaggcag acgcccaacc acacttggcc tgggcgatgg
    17941 gccaaacgat gcgcctttac tggaggtaat ggattacgcg gtgattgtga aagggctaaa
    18001 ccgtgaaggg gtacatctgc atgatgagga tccggcccgc gtctggcgaa cgcagcgtga
    18061 aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgcccgtt aagcgttatc
    18121 gctcgcgaat acccgattgc gcccggcctg ttttgcgagg taaagccgac ggtcggccaa
    18181 tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta cccccagcga
    18241 ggcactgatg cgtatcgttg tactcttggc gatcagcatc tctttttcat ttaacttcag
    18301 gcgaatacgt tctgcgacct ccgcagcctg cgtcagattc gcgcctggca gaatcacaca
    18361 aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac tgctaattaa
    18421 tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt cattaatcgc
    18481 tttaaaatgg tcaagatcga cctgaatgac agaaaaagga tgttggtgcg tctgacacaa
    18541 tttagcgagc ggacgggctt tttcgaacag tgcgccacgg ttatataaac gcgttaaggt
    18601 gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc tgaccatccg
    18661 gcgaatcaca taccaggaga ggagtaacat ggtggtaaag agcgcccaca gcagggttaa
    18721 tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat ggacgcgcgc
    18781 cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt ccatacgaat
    18841 gccgccccgc gtgtcatgtt ccattgcctg cgccagcaag gccagttcac gaggatcaaa
    18901 aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct tactgtcata
    18961 gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt gctgcatagt
    19021 acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt ctaccggaac
    19081 actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag tgaaccagcg
    19141 taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt gggtgacata
    19201 gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg aaacgtaaaa
    19261 tccagcacgc gagacataca tcgcctgttc aaccatcgac gaggagttgt gcgccagtcg
    19321 cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt cattttcgcg
    19381 agacaggaaa ttcccctcgc tgactaatgc atccgatacg ccattgactg gcagggtgcg
    19441 ccgtcggttg agttcgattt gccaggcgtg ctcgtcgcga tgctgttcga actcggttac
    19501 ggcattacgc agagaggtga aatccagtgg cgccacgaga gcttcgcgca tgccattacg
    19561 caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt tggctacgtt
    19621 ttccagatga ttacgctagc tggagatata ggcatcttcc agcaccacca cttcacgcca
    19681 ggtgagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga catgaccggg
    19741 ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg tctcgtgctg
    19801 caccctgacg actcccaacc tgctttttta tgattctggc ataacccggc aatagcgtac
    19861 cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc attcattgca
    19921 ggcattggga caccaggccg gatgtgatcc ggcctggtgt taatgatacc ctttcaaact
    19981 tcttccgcag aacgactccc ctcccccgga acaagatcgg tttcatgaaa agcttcccgc
    20041 ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta tccagtgaca
    20101 atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa gctcatactc
    20161 gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat tttgcataat
    20221 agccagtgcg agtattaatg tgcctgaccg cgcattttcg ctactgcttt cactcccagc
    20281 accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg taatatcatc
    20341 gccaccactg cactttgctg cccggcgata tgttcaatag cgtgatgcag cggcgcaata
    20401 ccatgtacca caatcccgcc gccgacgagg aacatcgcca gcgtgccgac aatcgataat
    20461 gctttcatca gccaaggcgc gataatcaat aaccctttac ctaatgcctg catcagcgcg
    20521 ctagattttt ccgccagcca ataccctagg tcatcaatct taacgataac ccctaccaga
    20581 ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac ctgattaagc
    20641 aacggcgcct ctgccacaat ccccagcgtg atggcgacga tttccgcaga caaaataaaa
    20701 tcggtacgaa tcgccccttt aattttgtcc ttttcaaact tcagcggatc ctgcgccgcc
    20761 agcttctcca gacgctgctg gctctgcgct ggatcttctt tatgtttacg cgcctccagc
    20821 atatgcagca ctttctctac cccttcaaag cagagaaacg cgccaccaat catcaacagt
    20881 ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac cagaatcact
    20941 ttattgatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg gttggcccgc
    21001 acgcctgaaa cttgttgcgc attgagcgat aaatcatccc ctaatacacc ggcggttttc
    21061 ttcgccgcca gtttgcccat cacagagata tcgtccagca gtgtggcgat atcatcgagc
    21121 aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg ttgaagggtg
    21181 taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca tttatttaac
    21241 aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact atgaaccacc
    21301 tttttatttc cgccgtgagg gattatgcgt ttccgccagt tgttaccgct ttttggcgcg
    21361 ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat tggcgtggga
    21421 agctggcctc cgttaatgat ggcgggcgtt cgattccagg cagccggtat tttattgctg
    21481 gcatttttgc tactgcgcgg acacaaactt cccccgctac gtccgctgct caatgccgcg
    21541 ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc cgaacatcaa
    21601 aatgttcctt ccggcatcgc cgccgtagtg attgcaaccg tgcccctctt taccctgtgc
    21661 ttcagtcgcc tgtttggcat taaaacgcgc aaactggaat gggtgggtat tgccattggg
    21721 cttgccggaa tcatcatgct caatagcggt ggaaatttaa gcggcaatcc gtggggcgcg
    21781 attctgattt taatcggctc gattagctgg gcgtttggct cagtttatgg ctcgcgcatt
    21841 accttacctg tagggatgat ggcgggtgcg attgagatgc tggcggcagg cgtggtgtta
    21901 atgatcgcat cgatgattgc gggtgaaaaa ctgacggcgc tcccttccct gtcaggcttc
    21961 cttgcggtcg gctatctggc gctgtttggt tcgattatcg ccattaacgc ttatatgtat
    22021 ttaatccgta atgtcagtcc ggctctcgcc accagctacg cctacgttaa cccggtggtc
    22081 gcggtcttgc tgggtacggg actgggtggc gaaacgctgt cgaagattga atggctggcg
    22141 ctcggcgtaa ttgttttcgc ggtggtactg gtcacgttgg gaaaatatct cttcccggcc
    22201 aaacccgttg tcgcgccagt tattcaggat gcatcaagcg agtaaatgaa tcccctgcgt
    22261 gtcgatctgc gcgctggcac cttcgccgca gatccactct tccagacgct cggtaagcgc
    22321 cgcatccgtc agcttctcgc gcctacgtaa cgcacactcc cagacaatca gtacgcgcca
    22381 gccgagctcc tgcaagcgac tgatatcgcg gcgatcgcgt tcaacatttt tacctatctt
    22441 ctccagccag aactcagttc gagtcgcagg cactttaaac agatagcagt gatgatgatg
    22501 ccagaagcag ccgtgggtaa atatcacgca gcgatattca tcaacgacaa aatccgggcg
    22561 tccgggcaga ctggcgtcct gaacgcgaaa cgcaaggccc tgcccggtta acaaactggc
    22621 gaggcgcttc tcaatcgccg tatcacgcgt ggcaatcgcg cgcatatttt tgctgcgggt
    22681 ggccttatcg tgaacgtcga ccatgttgtg cctcttgctg acgcaacgcc accgcctgtt
    22741 tgatttttgg ctcaagcagt tttgccaccg cggcaaagac cggcacgacc accgagttgc
    22801 cgaactggcg ataggcctga gtgtccgaaa ccggaatgcg gaatttcgct tctcccggcg
    22861 cttcaaaacc cattaagcgc gcgcattcac gaggcgttaa ccgacgtgga cgatgttgct
    22921 gattcagcgg atcgtcaaag tctttctcac ccgtggccat atcccaaccg cgatcgatta
    22981 aaatttccgc gccatctttg taataacgag cagacagcgt acgggtgacg ctttgcggat
    23041 tgttcggata aaccattcca taaccgaagc cgttaccgcg cgcctgatgt tttttcgcat
    23101 atcgatagag gtacttccac agcaccggcg tcaggatata tttcgcctca accatcgggt
    23161 ccagcagctg cgccagcgtc actcgctgtg cagggaaaca ttcgctgata tcacgcagag
    23221 taaaatcggc tttcagattc agatcgcgac gaaaacccac cagcacgatg cgttcacggt
    23281 gctgcggcag gaaatgttta ccatcgatga ttttcggatc gtccggcccg ttatcttctg
    23341 catcggcaac gtcatagccc agttcgtcca gcgtctgcat gatgatgcgg aacgtttttc
    23401 ccttatcgtg actcttcagg tttttgacgt tttcgagcac aaacatcgcc ggacgacgcg
    23461 cgtcgataat gcgcaccaca tcaaagaaca gcgtgccctg ggtatcgcag gcaaaaccgt
    23521 gcgcccgccc gagcgagttc tttttcgata cgccagccag cgaaaatggc tgacaaggga
    23581 aaccggccag taaaacatcg tgttcaggaa tgtgttgacg aatatgttcc gccgccgcct
    23641 catcactcac gccttcttga tgactgaggg tgatgtcgcg gatatcttca ttaaaatgat
    23701 gcgtcgccgg gtcgcaataa tggttggctt tataagtgcg taccgcatgt ttgttccatt
    23761 cgctggtaaa cacgcactgt ccaccaatcg attcaaaacc acgacggatg ccgccaattc
    23821 cggcaaatag atcgataaag cgaaacgcat aatgcggatg atgttccggt ggtttgggta
    23881 acaacgtttg cagatgggca aactctttct cacttaaacg gtgccatgcc gagtcattcg
    23941 ccagcgcacg ttttaaaatt gccgcgctcc agtgattctc acccacacca ttaagctgcg
    24001 ccaccagcat tttaacatca taaatttgca gcagtttctc cagcattgcc tgtgcggcat
    24061 tcccggtgct gtatgaatcg gttactgata tattttcctg catagattta accggccatc
    24121 tgagatggcc gacaggttaa cataattact gccgtttagg gccacgcgct cagccagcgg
    24181 gtgaaaacgc atctatcacc ttgtgatcga taccttcatt ctcccccgcc agttcggcac
    24241 cgagcttcgc catcaaacag cgccaagctc aatgcccctg aaacggcaaa tacgcgcgcc
    24301 aggccaatcg cccccagggc ttccagccga tccgcatcct ggacgatttt agcttccgtt
    24361 gttaaggggg cgatttgcgc gctgaaactg tgagcggcaa tggcatgaca aacggcctca
    24421 attttctccg ccggaaattg cacgaactct tcacggagca ggcgacgcgt ctcttctgcc
    24481 gccaggattg aagaacgctg ccgttgcgga tgatttttcg ccaggctcac gatatcgtga
    24541 aaataacagg cggttaaaat caccagcata tcgacatcgt catctgccgc cagtttctgt
    24601 gccgtagccc agacacggcg aaaatgacac aggtcatgcg ccgcgtcctg atgttgatga
    24661 tggtttttca accagttctc gaactgtgcc tgccagtgtt gtaactccat acgcgctccg
    24721 ttgccaaaga gtcgcaacct tagcagtttt tattcatagc gtctgcaacg tgcgggacga
    24781 ggtcgggtgt accatgcaat gccgcctaac aaggcaccaa ccagggccat aaacagaaat
    24841 ccgaccaatg ctcctaacca cttccccgcg gtagatccta aatcactttc aaatcccgac
    24901 acgggggcat cggggatccg gctcatcacc cagatgtatc gcgccattgc ccagacgaaa
    24961 tagccacacc atgtatagaa agc
//
LOCUS       DAJVRP010000059        24600 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVRP010000059 DAJVRP010000000
VERSION     DAJVRP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.059"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(1..374)
                     /locus_tag="OQN12_002434"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="OQN12_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="OQN12_002435"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="OQN12_002435"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1654721.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="OQN12_002436"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="OQN12_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1654722.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="OQN12_002437"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="OQN12_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1654723.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="OQN12_002438"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="OQN12_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1654724.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="OQN12_002439"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="OQN12_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1654725.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="OQN12_002440"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="OQN12_002440"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1654726.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="OQN12_002441"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="OQN12_002441"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1654727.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="OQN12_002442"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="OQN12_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1654728.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="OQN12_002443"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="OQN12_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1654729.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="OQN12_002444"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="OQN12_002444"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1654730.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="OQN12_002445"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="OQN12_002445"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1654731.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="OQN12_002446"
     CDS             10736..11161
                     /locus_tag="OQN12_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1654732.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="OQN12_002447"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="OQN12_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS1654733.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="OQN12_002448"
     CDS             12185..13621
                     /locus_tag="OQN12_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1654734.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="OQN12_002449"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="OQN12_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1654735.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="OQN12_002450"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="OQN12_002450"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1654736.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="OQN12_002451"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="OQN12_002451"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1654737.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="OQN12_002452"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="OQN12_002452"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1654738.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="OQN12_002453"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="OQN12_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCS1654739.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="OQN12_002454"
     CDS             complement(20454..20747)
                     /locus_tag="OQN12_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1654740.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="OQN12_002455"
     CDS             complement(20744..22033)
                     /locus_tag="OQN12_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1654741.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="OQN12_002456"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="OQN12_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="OQN12_002457"
     CDS             complement(23047..23811)
                     /locus_tag="OQN12_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCS1654742.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24600
                     /locus_tag="OQN12_002458"
     CDS             24127..>24600
                     /locus_tag="OQN12_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCS1654743.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLA"
BASE COUNT         6094 a         5940 c         6465 g         6101 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaatgaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cgggggggtc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca
//
LOCUS       DAJVRP010000060        24180 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVRP010000060 DAJVRP010000000
VERSION     DAJVRP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.060"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(22..594)
                     /locus_tag="OQN12_002459"
     CDS             complement(22..594)
                     /locus_tag="OQN12_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCS1654744.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OQN12_002460"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OQN12_002460"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1654745.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OQN12_002461"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OQN12_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1654746.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OQN12_002462"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OQN12_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1654747.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OQN12_002463"
     CDS             complement(3556..5337)
                     /locus_tag="OQN12_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1654748.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OQN12_002464"
     CDS             complement(5330..7102)
                     /locus_tag="OQN12_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1654749.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OQN12_002465"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OQN12_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS1654750.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OQN12_002466"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OQN12_002466"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS1654751.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8661..10353
                     /locus_tag="OQN12_002467"
                     /pseudo
     CDS             8661..10353
                     /locus_tag="OQN12_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10418..11113
                     /gene="queC"
                     /locus_tag="OQN12_002468"
     CDS             10418..11113
                     /gene="queC"
                     /locus_tag="OQN12_002468"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1654752.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11165..11563)
                     /gene="fadM"
                     /locus_tag="OQN12_002469"
     CDS             complement(11165..11563)
                     /gene="fadM"
                     /locus_tag="OQN12_002469"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1654753.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11657..12028)
                     /locus_tag="OQN12_002470"
     CDS             complement(11657..12028)
                     /locus_tag="OQN12_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1654754.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="OQN12_002471"
     CDS             complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="OQN12_002471"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1654755.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14242..14514)
                     /gene="hupB"
                     /locus_tag="OQN12_002472"
     CDS             complement(14242..14514)
                     /gene="hupB"
                     /locus_tag="OQN12_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1654756.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14723..17077)
                     /gene="lon"
                     /locus_tag="OQN12_002473"
     CDS             complement(14723..17077)
                     /gene="lon"
                     /locus_tag="OQN12_002473"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1654757.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="OQN12_002474"
     CDS             complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="OQN12_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1654758.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="OQN12_002475"
     CDS             complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="OQN12_002475"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1654759.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19535..20833)
                     /gene="tig"
                     /locus_tag="OQN12_002476"
     CDS             complement(19535..20833)
                     /gene="tig"
                     /locus_tag="OQN12_002476"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1654760.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="OQN12_002477"
     CDS             complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="OQN12_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1654761.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21799..22377
                     /locus_tag="OQN12_002478"
     CDS             21799..22377
                     /locus_tag="OQN12_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1654762.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22421..23896
                     /gene="ampG"
                     /locus_tag="OQN12_002479"
     CDS             22421..23896
                     /gene="ampG"
                     /locus_tag="OQN12_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1654763.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5665 a         6664 c         5827 g         6024 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcattcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 taatattgtt ccgggtttat atgcgattgc tcaaccgtct taaccagtat caacgtctgt
     8701 ggcaaccttc agccgcaaac cgtcaccgtc agcgaactgg ccgaacgctg tttttgcagc
     8761 gaacgccatg ttcgtacgct gttgcgtcag gcacaggagg cgggatggct ggagtggcag
     8821 gcgcagtcag gacgcggaaa gcgcggacaa ttacgctttc tggtcacgcc ggaatcgcta
     8881 cgcaatgcga tgatggaaca ggcactggaa accggaaagc agcaagatgt gctggagctg
     8941 gcgcaactgg ccccaggtga gctgcgcact ctgttacagc catttatggg cggacaatgg
     9001 caaaacgata cacccacgtt gcgtattccc tactatcgcc cgctcgaacc gctacaacca
     9061 ggctttttgc ccggccgtgc cgagcagcat ctcgccgggc agatattttc cggcctgacc
     9121 cgcttcgata ataatactca gcgcccgatt ggcgatttag cgcatcactg ggaaacctct
     9181 actgacaggt tacgctggga cttttatctt cgttcaaccc tacactggca taacggcgat
     9241 gcagtaaaag cctcacactt acaccagcga ttattgatgc tgttacaact gccagcactg
     9301 gatcaattat ttattagcgt gaagcgtatt gaagtcaccc atccgcagtg tctgaccttc
     9361 tttttacatc gccccgatta ctggcttgcg caccggctgg cgagctattg cagccatctg
     9421 gcgcatccgc aattcccact gatcggcact ggtccttttc gcttaacaca attcacagcg
     9481 gagctggtgc gcctggagag ccatgattat taccatttgc gtcatccgct gcttaaagcg
     9541 gttgagtact ggataactcc gccgcttttc gaaaaagatt tgggaaccag ttgtcggcat
     9601 cccgtgcaaa tcaccatcgg caaaccggag gagctgcaac gggtcagcca agtcagtagt
     9661 ggcatcagtt taggtttttg ctatttaacg ttgcgcaaaa gtccccgact ctccctctgg
     9721 caggcgcgaa aagtgatctc cattattcat caatccggtt tattacaaac gttagaagtc
     9781 ggagaaaacc tgatcaccgc cagtcatgca ttactgctag gctggactat tccgcactgg
     9841 caagtaccgg atgaagtcaa actaccgaaa accttgacgc tggtttatca cctaccgata
     9901 gaacttcata ccatggcaga acgcctacag gcgacactgg cagcggaagg ctgtgaactc
     9961 acaattattt ttcataacgc aaaaaactgg gacgacacga ccctactggc acacgcagac
    10021 ctcatgatgg gcgacagatt aattggcgaa gcaccggaat atactctgga gcaatggcta
    10081 cgttgcgatc cactatggcc acatgttttc gacgctccag catatgcaca tctgcaatcg
    10141 acgctggacg cggtgcaagt aatgcctgat gaggaaaacc gatttaatgc cctgaaagcg
    10201 gtttttagcc agttaatggc agacgcgacg ctgacgccgc tgttcaacta tcactatcgc
    10261 attagtgccc ctcccggcgt gaacggtgtg cgactgacac cgcgcggctg gtttgaattt
    10321 accgaagcct ggcttcccgc gccgtcgcaa tgaatagctg gtccgggtta atcgcaggcg
    10381 ttaccataac gcttttattg ttcaagcagg attatctatg aaacgtgctg tcgttgtgtt
    10441 cagtggaggc caggattcca ccacctgtct ggtgcaggca ttacaacaat atgatgaagt
    10501 ccattgcgtg acgttcgatt acggtcagcg acatcgcgca gaaatcgacg tggcacgcga
    10561 actggcgctg aaactgggag cacgcgcgca taaggttctt gatgtaacgc tgctcaacga
    10621 gctggcggtc agcagcctga cacgtgacag cattccggtg cctgattatg aacctgaagc
    10681 cgatggcatc ccgaatacgt ttgtcccagg gcgtaatatt ttgttcctga cgctggcggc
    10741 aatatatgcg tatcaggtaa aagcagaagc cgtaattacc ggcgtctgtg aaacggattt
    10801 ctccggctac ccggattgcc gcgatgagtt tgtgaaagca ctaaaccatg ccgtcagttt
    10861 gggcatggcg aaagatattc gttttgaaac gccgctgatg tggattgata aagcggaaac
    10921 ctgggcgctg gcagattatt acggcaaact ggatttagtc cgtaacgaaa cgttgacctg
    10981 ctataacggc attaaaggcg acggttgcgg tcattgtgcg gcatgtaatt tacgcgccaa
    11041 cggtttgaat cattatctgg ccgataaacc gacggtgatg gcagcgatga agcagaaaac
    11101 cgggttgagg taattattcc gggtgtcgcc ggatgcggct tgagcatccg gcaccacaaa
    11161 acgtttactt aaccatctgc tccagctttt cgcgcaattc cccttccaga gctaatgctt
    11221 tctgcgtttt aagatcaata caaacaaacg taataagcgc atccgctacc acctgccctt
    11281 ccggctccag tgtaatgacc tggtttaaga tgccgctttt accgtttaat tgctgcaact
    11341 gactggtaat agttaacagg tcgcttaata ccgctgggcg acgatagtta atattgatat
    11401 tgaccacgac gaaggcgatg ttatgggccg tcatccactg aaaactgtcg ctattttcca
    11461 ccccatccca gcgggcttct tcgagaaatt caaggtagcg ggcgttgttg acgtgctggt
    11521 aaacgtcgag atgatatcca cgaactttga tttgtgtttg catagcgcaa taaccttacg
    11581 ttgttgttat aagtacagag gtcataactg gtatgacctc ttcagtctgg caaaaaattg
    11641 ccactatgca aattaattac agggttaata ccgccagatt acgttccacc agcgaattgc
    11701 ccatccccgg cacctgcttt agatcctcca ccgttttaaa cggaccgtac tcttcgcgat
    11761 aactgacaat cgcctgcgct ttcttcaggc caacgccatt catcgcgcgg gctagctctt
    11821 ccgcgctggc attattaatg ctgacccggg tgccttcttc gtcactggct ttcgccggta
    11881 ctgctgcttt actttgtgct gcaggagctt ccgctttggt ttctaccgcc gtcggtttcg
    11941 ccacagaagc tgccgccagc gcgctatgag acattccggc acaggccagg gacagggtaa
    12001 tgagcagtgc tttaattccg tgtttcatac tgttttctcc ttgtttgtta acagtggaat
    12061 caccttaacg ggagtgaaaa cagtaaacaa atggcaatgt tcagaaatgg aaaaggccgc
    12121 gaaagcgacc ttttcttgtt acagctgcaa cgcatttttt tgcgaggcgg cttcaggatt
    12181 attgctgttc cagcgcatcg ccaattttga ttttcgcctc tttacgcagg ttactcatca
    12241 gagcttcaaa gacgatttgt gcgttgttct gggtgatacc ctgcaccatc gcttttttct
    12301 gatcttccgg cattgaacct tgtttcactt catccagcgc cagcagaacc acattacctt
    12361 gcatatcggt cgccataccg tagctcggtt tgtctttcgc tggcagtggc agtgcaaacg
    12421 ccgcctggct aatcgggtca cgaccggaac ggcttaaggt tttcggctcg ccaaatttca
    12481 gaccggcagc ctgcatagct tccgcacctt tgccggcttt caaatcaacc agcagtttct
    12541 cagcatccac tttcgcctgt tgttcagctt tgttgtgctg aaccagtgcc ttaacttgtt
    12601 cctgaacatc tgccaacggt ttcaccgctt ccggtttgtg ctcgctgacg cgcagcacga
    12661 atgcgcggtc gccgtctacg gtgatgatgt cagagttgat gcccgacgcg ccgttttcac
    12721 ctaccagacc gccgttaaag atagcgtcgg caaccggctt gaagttcaac tcttccggca
    12781 ggttatcttt gctgaaccaa cccgtctgag tggctttaac gccggcagct tgctctgcac
    12841 cggccagaga ctcggtgtcg ttgcttgccg catcgctcac tttctgctgc agcgcatagt
    12901 acgcatcgag ggctttttcg tgtttcactt tcgccgcaat gtcgtcacgt acttcgtcta
    12961 acgatttcac tttcgctggc tgaatgtcgt ccagacgtac aatcaggaaa ccgaccgaag
    13021 atttgatgac accagacagt tggccttttt ctttcagacc agcatttttc agttcgtcgg
    13081 ggatagtggc atcttctaac caacccatat cgccgccgtt acgggcagag ataatatcgg
    13141 cagatttttc tttggctaat gcagcaaaat caccgccttt attcagctca tcaagtaccg
    13201 ctttcgcttc atcttcagtt ttggtctgga tgatgctgta gcgggtacgc tgcggctggg
    13261 tgaattgatc ctgatgctga tcgtagtagc tctggatatc cgcatcgcta accggttgct
    13321 gcatcgttgc ggcaaccagc ttgatgtaac tcacgcggaa ttgttccggc gtcatgaaat
    13381 tgtttttgtt ttgttcgtag tagctggcaa tctcctgttc ggtcacaggc tgcttcgccg
    13441 ccagcgcgtt aacatcgata gtcgcctcac gcaccacgcg ttgttgcgcg accagtgccg
    13501 ccagctcgtc ggtttcacct ttcagcataa aatcggtacc ggcaacgccg ttaatcagct
    13561 gttgggtagt gagctggtta cgcagcgcct gggcgtactg atcggcggtc atccccatct
    13621 ggttgaggat accgttatag cggctgttat caaatttgcc gtcaacctgg aaggctgggg
    13681 tcgcgaaaat cgcctgttta acctgctcat cactgatacc cagtttcagc tcacgagcgt
    13741 actgatccaa cagcgcctcg tcgatcagac gattcagcac ctgttgacgc agggttttca
    13801 tatagccttc gttcgctgcc agctcggagt attgatcgcc cagctgttgc tgcatacgat
    13861 tacgctcgct gttaaaggcg ttctcgaact gcccacggct gatttcctgg tcattcactt
    13921 ttgcggcgta gttattgcct ccgccaatca ggtaaccact cacgccggtc aatatgaacg
    13981 acacgataat gataccgaaa ataatcttga gcacgagact gtttgcagcc gtgcgtaagc
    14041 tgtccatcat ggtgtaacaa cactccgctg tagatgactg tgtacctcac gcaacgtatc
    14101 gatgcgcgca aaggcctatt gtgacaagaa acgggggcaa ttgtcagccc acaagacaca
    14161 gaaagtcacc gaatacaaat aaaaaaggca catcagtaga tgcgcccttg aacttcgtca
    14221 catccccact ggggacaacg cttagtttac cgcgtctttc agtgctttac ctgcacggaa
    14281 gctcggtact ttagcggcag cgatggtgat ctctttaccg gtctgcgggt tgcggccagt
    14341 acgggcagca cgctctttaa cggcaaaagt accaaaacct accagtgcta catcatcccc
    14401 ttctttcaga gattcagtta cggaagcaat aatagcatct aacgcacggc cagccgcagc
    14461 tttagagata tcagcccctg cagcaatctt gtcgatcaat tgagatttat tcactcttct
    14521 cttcctcttt ataatttata tcgcacttga atccttcaag gtacgaacgc gcagcagtta
    14581 tatcaggcca gccatcccct tacaacaccc cttaataagg gcaagcccga tccgctatct
    14641 aacttagcga gacaaaaaaa ggctggcaag cccgaattag cctgccagcc ctgtttttat
    14701 tagtgcattt tgcgcgaggt cactattttg cagtcacaac ctgcatgcca gacggttcat
    14761 tttgcagcgc cagagtcaga acttcctcaa tgcgcttcac aggatgaatg tccagatcgg
    14821 caattacgtt gtcaggaatc tcttccagat cgcgtttatt ttcgaacgga attagcactg
    14881 ttttaatccc gccgcgatgc gctgccagga gtttttcttt caaaccaccg atcggcagta
    14941 cctgaccacg cagagtgatc tcaccggtca ttgccacatc ggcacgaacc gggttaccgg
    15001 tcaggcaaga aaccagcgcg gtgcacatag caataccggc actcggacca tctttcggcg
    15061 tcgcaccttc cggtacgtgg acgtggatgt cacgtttttc gtaaaaatca gggttgatcc
    15121 ccagtttttc cgcacgcgca cgaaccaccg ttaacgccgc ctgaatagac tcctgcatca
    15181 cttcgccgag cgaaccggta taggtcagtt tgcctttacc cggaacacat gcggtttcaa
    15241 tggtcagcaa gtcaccgccc acttccgtcc acgccagacc gattacctga ccgacacggt
    15301 tttcgttatc agcgcgacca tagtcgaaac gctgaacacc gaggtagtca tgcaggttat
    15361 cgccgttaat ttcgatatgt tttaatgact tatcgagcag taactgctta accgctttgc
    15421 ggcacagttt ggagatttca cgctccagac cacgcacgcc cgcctcacgg gtgtagtaac
    15481 gaataatgcc gataatggcg ctatcgtcga cggtcagctc accttttttc agtgcattac
    15541 gttcaatctg cttcggcagc aggtgacgtt tggcgatgtt cagtttttca tcttcggtat
    15601 aaccggagag gcgaatcact tccatacgat ccagcagcgg tgccggaatg ttcatggagt
    15661 tcgacgtcgc gacaaacatc acgtcgctga gatcgtaatc cacttccagg tagtggtcgc
    15721 tgaacgctac gttctgctct ggatccagca cttcaagcag tgcagaggcc ggatcgcctc
    15781 gcatgtcaga agacattttg tcgatctcat cgagcaggaa cagcggattt ttcacgccca
    15841 ctttcgccat tttctggatc agtttacccg gcatagaacc gatgtaagta cggcggtgac
    15901 cacggatttc cgcttcatca cgcacgccgc ccagcgccat acggacatat ttacgcccgg
    15961 tggctttggc aatggactga ccaagagagg ttttacctac ccccggcggc cctaccaggc
    16021 agaggatcgg tcccttgatt ttgttgacac ggctttgaac cgcaaggtac tcaaggatgc
    16081 gatctttcac gcgctccaga ccataatggt cggtatcaag gatttcctgc gcctgacgca
    16141 ggtctttttt gaccttgctg cgcgcattcc acggtacctg taccatccag tcgatataac
    16201 cacgcactac ggtcgcttct gccgacatcg gagacatcat tttcagcttc tgcaactctg
    16261 cttccgcttt ctcttttgcc tctttcggca tcttcgccgc gtcgattttg cgcttcaggg
    16321 cttcgttttc gtccggcacg tcgtccattt caccgagttc tttctgaata gctttcattt
    16381 gctcgttcag atagtactca cgctgggatt tctccatctg ctttttaacg cggttgcgaa
    16441 tgcgtttctc aacctgcagc agatcgattt ccgattccat cattgccatc agatattcca
    16501 gacgttcgtt aacgtcggac atctccagaa cggactgttt gtcagccagt ttcagcggca
    16561 tatgtgcagc aatggtatcc gccagacgcg ccggatcgtc gatgctattc agcgacgtca
    16621 gcacttctgg tgggattttt ttgttcagct tgatgtagcc ttcgaactgg ctgattgcag
    16681 tacgcaccag cacttcctgt tcccgctcat caatggtcgg cgactccaga tactccgcct
    16741 tcgcagaaaa gtgttcgcca ttgtcagaga gcgcagaaat acgcgcgcgc tgtaacccct
    16801 cgaccagcac tttgacggtg ccgtcaggca gtttcagcat ctgcaatata gaggccacgg
    16861 tcccgacggt gaaaagatcg tttacacccg gctcatccgt tgaagcttct ttctgcgcga
    16921 ccagcataat ttttttatca tggtccatcg ccgcttccag acaacggata gatttttccc
    16981 gcccgacaaa taaggggatg accatgtgcg gataaaccac cacatcgcgc agcggcaata
    17041 cggggatttc aatgcgttca gaacgctcag gattcataga gctctctctt agtttaattt
    17101 ccgccaggta atcagatgac acgactgtgc ttcacgccat ctattaacat gtacgtcagt
    17161 atatggggat gtttccccca cattcaacgc cgagaataga ggaaaaatta aaggggagat
    17221 aaaatccccc ctttttggtt aactaattgt atgagaatgg ttaattattc accagatgcc
    17281 tgttgcgctt ccggcttacc ataaatcagc aacggtttgc tttgaccatc aattaccgac
    17341 tcgtcgataa ccactttttc gacgtcttcc atggacggca gatcgtacat ggtatcgagc
    17401 agtgcggctt ctacgatgga acgcaggcca cgggcaccgg ttttacgcgc catcgctttc
    17461 ttagcgatag catccagcgc ctcgtcacgg aattccagat ccacgccttc cagattaaac
    17521 agcgcctgat actgcttggt cagggcgttt ttcggctctt tgaggatctg aatcagagct
    17581 tcttcgctca gttcattcaa cgttgctaca accggcagac gaccaatgaa ctcagggata
    17641 agaccaaact tgatcagatc ttccggttca acctgcgcca gcagctcgcc ttcgcttgct
    17701 ttgtcggact tcgcttttac cgtcgcgcca aaaccaatgc cggagccggt ttctacacgg
    17761 tgggaaatca ctttatccag accggcaaac gcaccgccac aaataaacag gatcttagag
    17821 gtatcaacct gcaagaattc ctgctgcgga tgtttacgcc caccttgcgg tggaacagca
    17881 gctaccgtac cttcgatcag tttcaacagt gcctgctgta ctccttcacc ggaaacgtct
    17941 cgggtaatgg acgggttgtc tgacttacga gaaatcttgt cgatttcatc gatgtagaca
    18001 atcccgcgct gcgctttctg tacgtcgtaa tcgcactttt gcaacagctt ctgaatgatg
    18061 ttttcaacgt cttcgcccac ataaccggct tcggtcagcg tggttgcgtc ggccatggtg
    18121 aacgggacgt ccagcaggcg cgccagcgtt tcagccagca gcgttttacc ggaaccggtc
    18181 ggaccgatca gcagaatgtt acttttgccc aactcgacac cattgctggt atcgccgttg
    18241 cgcagacgtt tgtaatggtt gtataccgcg accgccagca cttttttcgc ctgttcctgg
    18301 ccgataacgt aatcgtccag gtggttgcga atttcatgcg gcgtcggtag cgcactgcgt
    18361 tcacgatgcg gtgcaacttc tttaatctct tcgcgaatga tgtcgttaca taaatcaaca
    18421 cattcgtcgc agatatacac ggatggaccg gcaatcagct tgcgcacttc atgctggctt
    18481 ttgccgcaaa aagagcaata cagcaatttg cctgagccat ctttgcgttt atctgtcatg
    18541 ggtcaaaacc tcttctttgt tctttgtgcc gcacacgacg acgcaaatgc cattctcagg
    18601 cgcaagccgc ttacagcgtt gtgccgccct agataagtat agcggcacag ttgcgcctct
    18661 ggcatcaatt acgatgggtc agaatcgaat cgaccagacc gtattccacc gcttcagggg
    18721 cggaaaggaa gcgatcgcgc tcggtatcac gttcaatctg ttctaatgat tgacccgtat
    18781 gaagcgccat aagttcattc atgcgccctt taactttcag aatttcacgg gcatgaattt
    18841 cgatatcggt cgcctggccc tggtagccgc ccaacggttg gtgaatcatc acgcgcgaat
    18901 tcggcaggca aaaacgttta ccttttgccc ctgcggtcaa caagaaagcg cccatcgagg
    18961 ccgcctggcc catacagatg gtgctgacat caggcttgat aaactgcatg gtgtcataga
    19021 tagacatccc ggcagtgatc accccgcctg gggagttaat gtacagatag atatcttttt
    19081 ctgggttttc cgcttccagg aacagcatct gcgccacaat caggttagcc atgtggtctt
    19141 caacctggcc agtcagaaaa atgacgcgtt ccttaagtag acgagaatag atatcaaaag
    19201 agcgctcacc gcgtgaggtc tgttcaatga ccatcggcac cagcgccata tggggtgcaa
    19261 agttatctcg ttcgccgctg tatgacattt ccgtctcctg gataaaattg aaaaaaacct
    19321 gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg
    19381 catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga aagaggcttt
    19441 tcgcacggct ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc ggtgacgggc
    19501 ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc atcagctcgt
    19561 tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca acagcctgtt
    19621 cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg tagaactcga
    19681 taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct ttcacgcgct
    19741 cttcgtcagc tttcagctcg ttggtgcgga taacttcgcc cagcagcagg ccaacaacta
    19801 cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt
    19861 tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg tcgatcagcg
    19921 cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga gacttaacgc
    19981 ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca gcgcgcagac
    20041 cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc agttccggca
    20101 gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta cctttcaggt
    20161 tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg ccagctttgt
    20221 ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc gccagtacga
    20281 aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg aagtcgatgg
    20341 ttacgcggtc ttctgcttca acagcgccgt ctttttcttt ccaggtcgcc tgctgtttac
    20401 gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa
    20461 cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac tctacagagt
    20521 aagtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg ccagccggat
    20581 tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca cccagaacgt
    20641 cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact ttgcctttgc
    20701 ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta acagcggtct
    20761 cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga gtggtttcaa
    20821 ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca
    20881 agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac attccctgtt
    20941 accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca tcacttttat
    21001 gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg ggcaattttc
    21061 gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc gcaggccgtt
    21121 tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat gcgtttttac
    21181 gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg caacccttcc
    21241 cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga gagttcctcc
    21301 gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg
    21361 ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg acgatagctt
    21421 tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc ttctatccgc
    21481 tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca tccctttaaa
    21541 tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg tttcgtcctg
    21601 gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag gcctcgcatc
    21661 agcgtttctt ttaccagggg tgattatttc gtcaacatga tgaatttaca tgccttaccc
    21721 acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac acgcttaaga
    21781 gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg
    21841 cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat tacactgcca
    21901 cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga tcagcgtacc
    21961 gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac cgcctcccgc
    22021 gctctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg
    22081 gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta tgctgacgtg
    22141 tcccagggca acgtgcgcta caacatcgct accaaagcgg atatcgccat catcgctacc
    22201 gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg
    22261 ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac cgataccatc
    22321 gctgatatgt ctcaggacac cagcattcac gaattcatca agcagaacgc gcgttaattt
    22381 ctgccctctg gcccggtgca agccgggcct gtagacgccc atgtccagtc aatatttacg
    22441 tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt ccgggctacc
    22501 gctcgccctg acatccggca ccttacaggc ctggatgacg gtcgaaaata tcgatctcaa
    22561 aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc tctggtcacc
    22621 gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc tgctcgccac
    22681 gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaaccag gcacccaact
    22741 ccgctggatg gcggcgctgg cggtggtgat cgctttttgc tctgcctccc aggatattgt
    22801 cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg gcgcggcaat
    22861 cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcctagccc tgtggctggc
    22921 agataaatgg ttgggctggc agggcatgta ctggttgatg gcggcgctgt tgatcccctg
    22981 tattatcgcg acgttgcttg caccagaacc aaccgacacc attcctgtgc ccaaaacgct
    23041 ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg cctggcttat
    23101 tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc tgacaaccac
    23161 gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtaa acaaaacgct
    23221 tggcttactg gcgaccattg ttggcgcatt gtacggtggg attttgatgc agcgcctgtc
    23281 gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg ctggttactg
    23341 gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct ttttcgaaaa
    23401 cctctgtggc gggatgggca catcagcctt tgtcgcgctg ttaatgacgc tgtgtaataa
    23461 gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag ggcgagttta
    23521 tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat tctatctatt
    23581 ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc agacgcttga
    23641 atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccggcag gttatgcctt
    23701 tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac ttctgttgac
    23761 gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg aagtcggggt
    23821 tttagtcgcc ctttctggcg tcatgcttgg tggtttgctg gattatctgg cgctacgaaa
    23881 aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg ccgtaattat
    23941 tacggctaaa taaacatcag catcgcttat taatatttgt tcttttgtgc ggcttagcgt
    24001 ttggaaatta ttgacgccat ttataaattc tatttttctt acacgattca gctaatgagt
    24061 ctttattttc tcaccaccca gttgtcactt taatgataat tatttgttaa ataattgttt
    24121 tatttcgcat tggttatacc aattgcccgc caagactcct tctgcactcc ccttttgtta
//
LOCUS       DAJVRP010000061        23068 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVRP010000061 DAJVRP010000000
VERSION     DAJVRP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.061"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OQN12_002480"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OQN12_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1654764.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OQN12_002481"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OQN12_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS1654765.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OQN12_002482"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OQN12_002482"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1654766.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OQN12_002483"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OQN12_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1654767.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OQN12_002484"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OQN12_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1654768.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OQN12_002485"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OQN12_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1654769.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OQN12_002486"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OQN12_002486"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1654770.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OQN12_002487"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OQN12_002487"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1654771.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OQN12_002488"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OQN12_002488"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1654772.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OQN12_002489"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OQN12_002489"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1654773.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OQN12_002490"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OQN12_002490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1654774.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OQN12_002491"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OQN12_002491"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1654775.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OQN12_002492"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OQN12_002492"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1654776.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OQN12_002493"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OQN12_002493"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1654777.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OQN12_002494"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OQN12_002494"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1654778.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OQN12_002495"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OQN12_002495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1654779.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OQN12_002496"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OQN12_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1654780.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OQN12_002497"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OQN12_002497"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1654781.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19506..20327
                     /locus_tag="OQN12_002498"
     CDS             19506..20327
                     /locus_tag="OQN12_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCS1654782.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20314..22422
                     /locus_tag="OQN12_002499"
     CDS             20314..22422
                     /locus_tag="OQN12_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS1654783.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22419..>23068
                     /locus_tag="OQN12_002500"
     CDS             22419..>23068
                     /locus_tag="OQN12_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1654784.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLAL"
BASE COUNT         5841 a         5549 c         6005 g         5673 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc ttgtgggcgg
    19381 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta
    19441 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt
    19501 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag
    19561 aggggcgtgg ccaagggcat ggtaaagact atattccatg gctaacagta caagaagttc
    19621 cttcttcagg tcgttcccac cgtatttatt ctcataagac gggacgagtc catcatttgc
    19681 tatctgactt agagcttgct gtttttctca gtcttgagtg ggagagcagc gtgctagata
    19741 tacgcgagca gttcccctta ttacctagtg ataccaggca gattgcaata gatagtggta
    19801 ttaagcatcc tgttattcgt ggtgtagatc aggttatgtc tactgatttt ttagtggact
    19861 gcaaagatgg tccttttgag cagtttgcta ttcaagtcaa acctgcagca gccttacaag
    19921 acgagcgtac cttagaaaaa ctagaactag agcgtcgcta ttggcagcaa aagcaaattc
    19981 cttggttcat ttttactgat aaagaaataa atcccgtagt aaaagaaaat attgaatggc
    20041 tttattcagt gaaaacagaa gaagtttctg cggagctttt agcacaacta tccccattgg
    20101 cccatatcct gcaagaaaaa ggagatgaaa acattatcaa tgtctgtaag caggttgata
    20161 ttgcttatga tttggagtta ggcaaaacat tgagtgagat acgagcctta accgcaaatg
    20221 gttttattaa gttcaatatt tataagtctt tcagggcaaa taagtgtgca gatctctgta
    20281 ttagccaagt agtgaatatg gaggagttgc gctatgtggc aaattaatga ggttgtgcta
    20341 tttgataatg atccgtatcg cattttggct atagaggatg gccaagttgt ctggatgcaa
    20401 ataagcgctg ataaaggagt tccacaagct agggctgagt tgttgctaat gcagtattta
    20461 gatgaaggcc gcttagttag aactgatgac ccttatgtac atcttgattt agaagagccg
    20521 tctgtagatt ctgtcagctt ccagaagcgc gaggaggatt atcgaaaaat tcttcctatt
    20581 attaatagta aggatcgttt cgaccctaaa gtcagaagcg aactcgttga gcatgtggtc
    20641 caagaacata aggttactaa ggctacagtt tataagttgt tacgccgtta ctggcagcgt
    20701 ggtcaaacgc ctaatgcatt aattcctgac tacaaaaaca gcggtgcacc aggggaaaga
    20761 cgttcagcga caggaacagc aaagattggc cgagccagag aatatggtaa gggtgaagga
    20821 accaaggtaa cgcccgagat tgaacgcctt tttaggttga ccatagaaaa gcacctgtta
    20881 aatcaaaaag gtacaaagac caccgttgcc tatagacgat ttgtggactt gtttgctcag
    20941 tattttcctc gcattcccca agaggattac ccaacactac gtcagtttcg ttatttttat
    21001 gatcgagaat accctaaagc tcagcgctta aagtctagag ttaaagcagg ggtatataaa
    21061 aaagacgtac gacccttaag tagtacagcc acttctcagg cgttaggccc tgggagtcgt
    21121 tatgagattg atgccacgat tgctgatatt tatttagtgg atcatcatga tcgccaaaaa
    21181 atcataggaa gaccaacgct ttacattgtg attgatgtgt ttagtcggat gatcacgggc
    21241 ttttatatcg gctttgaaaa tccgtcttat gtggtggcga tgcaggcttt tgtaaatgct
    21301 tgctctgaca aaacggccat ttgtgcccag catgatattg agattagtag ctcagactgg
    21361 ccgtgtgtag gtttgccaga tgtgttgcta gcggaccgtg gcgaattaat gagtcatcag
    21421 gtcgaagcct tagtttctag ttttaatgtg cgagtggaaa gtgctccacc tagacgtggc
    21481 gatgctaaag gcatagtgga aagcactttt agaacactac aagccgagtt taagtccttt
    21541 gcacctggca ttgtagaggg cagtcggatc aaaagccatg gtgaaacaga ctataggtta
    21601 gatgcatctc tgtcggtatt tgagttcaca caaattattt tgcgtacgat cttattcaga
    21661 aataaccatc tggtgatgga taaatacgat cgagatgctg attttcctac agatttaccg
    21721 tctattcctg tccagctatg gcaatggggt atgcagcatc gtacaggtag tttaagggct
    21781 gtggagcaag agcagttgcg agtagcgtta ctgcctcgcc gaaaggtctc tatttcttca
    21841 tttggcgtta atttgtgggg tttgtattac tcggggtcag agattctgcg tgagggttgg
    21901 ttgcagcgga gcactgatat agctagacct caacatttag aagcggctta tgacccagtg
    21961 ctggttgata cgatttattt gtttccgcaa gttggcagcc gtgtattttg gcgctgtaat
    22021 ctgacggaac gtagtcggca gtttaaaggt ctctcatttt gggaggtttg ggatatacaa
    22081 gcacaagaaa aacacaataa agccaatgcg aagcaggatg agttaactaa acgcagggag
    22141 cttgaggcgt ttattcagca aaccattcag aaagcgaata agttaacgcc cagtactact
    22201 gagcccaaat caacacgcat taagcagatt aaaactaata aaaaagaagc cgtgacctcg
    22261 gagcgtaaaa aacgtgcgga gcatttgaag ccaagctctt caggtgatga ggctaaagtt
    22321 attcctttca acgcagtgga agcggatgat caagaagatt acagcctacc cacatacgtg
    22381 cctgaattat ttcaggatcc accagaaaag gatgagtcat gagtgctacc cggattcaag
    22441 cagtttatcg tgatacgggg gtagaggctt atcgtgataa tccttttatc gaggccttac
    22501 caccattaca agagtcagtg aatagtgctg catcactgaa atcctcttta cagcttactt
    22561 cctctgactt gcaaaagtcc cgtgttatca gagctcatac catttgtcgt attccagatg
    22621 actattttca gccattaggt acgcatttgc tactaagtga gcgtatttcg gtcatgattc
    22681 gaggtggcta cgtaggcaga aatcctaaaa caggagattt acaaaagcat ttacaaaatg
    22741 gttatgagcg tgttcaaacg ggagagttgg agacatttcg ctttgaggag gcacgatcta
    22801 cggcacaaag cttattgtta attggttgtt ctggtagtgg gaagacgacc tctcttcatc
    22861 gtattctagc cacgtatcct caggtgattt accatcgtga actcaatgta gagcaggtgg
    22921 tgtatttgaa aatagactgc tcgcataatg gttcgctaaa agaaatctgc ttgaattttt
    22981 tcagagcgtt ggatcgagcc ttgggctcga actatgagcg tcgttatggc ttaaaacgtc
    23041 atggtataga aaccatgttg gctttgat
//
LOCUS       DAJVRP010000062        22963 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVRP010000062 DAJVRP010000000
VERSION     DAJVRP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.062"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            29..238
                     /gene="fumD"
                     /locus_tag="OQN12_002501"
     CDS             29..238
                     /gene="fumD"
                     /locus_tag="OQN12_002501"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS1654785.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            694..1320
                     /locus_tag="OQN12_002502"
     CDS             694..1320
                     /locus_tag="OQN12_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS1654786.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1341..3442
                     /locus_tag="OQN12_002503"
                     /pseudo
     CDS             1341..3442
                     /locus_tag="OQN12_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3455..4093
                     /locus_tag="OQN12_002504"
     CDS             3455..4093
                     /locus_tag="OQN12_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS1654787.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4157..4825
                     /locus_tag="OQN12_002505"
     CDS             4157..4825
                     /locus_tag="OQN12_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1654788.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4822..5607
                     /locus_tag="OQN12_002506"
     CDS             4822..5607
                     /locus_tag="OQN12_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS1654789.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5611..6423
                     /locus_tag="OQN12_002507"
     CDS             5611..6423
                     /locus_tag="OQN12_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654790.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6435..8039)
                     /locus_tag="OQN12_002508"
     CDS             complement(6435..8039)
                     /locus_tag="OQN12_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCS1654791.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(8165..8470)
                     /locus_tag="OQN12_002509"
     CDS             complement(8165..8470)
                     /locus_tag="OQN12_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1654792.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8578..8654)
                     /locus_tag="OQN12_002510"
     tRNA            complement(8578..8654)
                     /locus_tag="OQN12_002510"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8618..8620),aa:Val,seq:gac)
     gene            complement(8659..8735)
                     /locus_tag="OQN12_002511"
     tRNA            complement(8659..8735)
                     /locus_tag="OQN12_002511"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8699..8701),aa:Val,seq:gac)
     gene            9043..10299
                     /locus_tag="OQN12_002512"
     CDS             9043..10299
                     /locus_tag="OQN12_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1654793.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10340..11713)
                     /gene="mdtK"
                     /locus_tag="OQN12_002513"
     CDS             complement(10340..11713)
                     /gene="mdtK"
                     /locus_tag="OQN12_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1654794.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11928..12569
                     /gene="ribE"
                     /locus_tag="OQN12_002514"
     CDS             11928..12569
                     /gene="ribE"
                     /locus_tag="OQN12_002514"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1654795.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12609..13757)
                     /gene="cfa"
                     /locus_tag="OQN12_002515"
     CDS             complement(12609..13757)
                     /gene="cfa"
                     /locus_tag="OQN12_002515"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1654796.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(14048..15259)
                     /locus_tag="OQN12_002516"
     CDS             complement(14048..15259)
                     /locus_tag="OQN12_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1654797.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15372..16304
                     /locus_tag="OQN12_002517"
     CDS             15372..16304
                     /locus_tag="OQN12_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1654798.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(16301..17326)
                     /gene="purR"
                     /locus_tag="OQN12_002518"
     CDS             complement(16301..17326)
                     /gene="purR"
                     /locus_tag="OQN12_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1654799.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17625..17714
                     /gene="ynhF"
                     /locus_tag="OQN12_002519"
     CDS             17625..17714
                     /gene="ynhF"
                     /locus_tag="OQN12_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1654800.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17880..19049
                     /locus_tag="OQN12_002520"
     CDS             17880..19049
                     /locus_tag="OQN12_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1654801.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19195..19776)
                     /gene="sodB"
                     /locus_tag="OQN12_002521"
     CDS             complement(19195..19776)
                     /gene="sodB"
                     /locus_tag="OQN12_002521"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1654802.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19904..20731)
                     /gene="mepH"
                     /locus_tag="OQN12_002522"
     CDS             complement(19904..20731)
                     /gene="mepH"
                     /locus_tag="OQN12_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1654803.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            21066..21413
                     /gene="grxD"
                     /locus_tag="OQN12_002523"
     CDS             21066..21413
                     /gene="grxD"
                     /locus_tag="OQN12_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS1654804.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21452..21952)
                     /gene="tnpA"
                     /locus_tag="OQN12_002524"
     CDS             complement(21452..21952)
                     /gene="tnpA"
                     /locus_tag="OQN12_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1654805.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            21960..>22963
                     /locus_tag="OQN12_002525"
     CDS             21960..>22963
                     /locus_tag="OQN12_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1654806.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     A"
BASE COUNT         5962 a         5343 c         5942 g         5716 t
ORIGIN      
        1 tagttggcat gataaatagg tataaagaat gggaaatcgg acaaaagagg atgaactgta
       61 ccgagaaatg tgcagagttg tcggtaaagt tgtgctggaa atgcgcgatc tggggcagga
      121 accaaagcat attgttatcg caggcgtatt acgtacagca ttagccaata aacgcattca
      181 gcgcagtgag ctggaaaagc aggcgatgga aaccgtcatt aacgcactgg taaaatgacc
      241 attcaggatg aggcggtaag cttcatcctg atattcttgc ttcagttgcg gtccagttaa
      301 tcattctaaa taacaactgc tgaaattata cgaatttcgg tatgtatttt aatttatagc
      361 gataatgtta tttttatttt ctgcaatgaa cgtcacaaat gattgtcgct taagttaaat
      421 ttatttcaaa tgataataaa tatcagttcg cttataattt cttgctttgt aattttaatt
      481 gataacgatc aatgttaagg gctcagaata atcacattat tttttctcct aaccataaag
      541 gattagttta tcggcgttaa atcttttctc atacactatc gggttaggga aaatatctgg
      601 tgttggggac agagtcagca gcgtattagt tgaatgctta atatcaggct gctgaattgt
      661 cagcactgtg tttgcgaaga ggatgcgaaa taaatgaacc cggttgatcg tccactatta
      721 gatattggct taacgcgatt agaattcttg cgtatatcag gaaaaggcct tgctggtcta
      781 acaattgccc ctgcgttact gtcacttttg ggttgcaaac aggaagatat tgatagcggc
      841 acagtagggt tgataaatac acccaaaggg gtgctggtga cccagcgagc gcgatgcacg
      901 ggttgccacc gctgtgaaat ctcttgtacc aacttcaacg atggctcagt agggacattc
      961 ttctcccgta tcaaaatcca tcgcaattat ttctttggcg acaacggggt tggctctggc
     1021 ggcggcctgt atggcgatct caactatacc gcggacacct gccgtcaatg caaagagccg
     1081 caatgcatga acgtctgccc gattggtgcg attacctggc agcagaaaga aggctgtatt
     1141 accgtcgatc ataaacgttg tattggctgt agcgcctgta ctacggcatg cccgtggatg
     1201 atggccaccg taaataccga aagtaaaaaa tcctcgaaat gtgtgttatg cggtgaatgc
     1261 gcaaacgcct gcccaacagg ggcgttaaaa attatcgagt ggaaagatat tactgtgtga
     1321 atcttgcaaa ggaaaaaatc atggctaacg gttggacagg taatatatta agagtcaatc
     1381 tcacgacagg aaatattacc ctcgaagatt ccagtaagtt taaaagtttt gtcggtggca
     1441 tgggcttcgg ctacaaaatt atgtatgacg aagtaccgct aggcacgaaa cctttcgatg
     1501 aagcgaataa attagtcttt gctaccggcc cattaactgg atctggtgcc ccctgtagtt
     1561 ctcgcgtaaa tatcacctca ctttctactt ttaccaaagg aaatttagtc gcgatgccca
     1621 tatgggtggc ttttttgcag cgcaaatgaa attcgctgga tacgacgtca ttattatcga
     1681 agggaaggcg aaatcaccgg tatggctgaa gattaaagat gacaaagtta gcctggaaaa
     1741 agccgatttc ttatggggaa aagggacgcg cgcaacgacg gaagaaattt gtcgattgac
     1801 cagcccggaa acctgtgtgg cggctattgg tcaggctggg gaaaacctta ttcctctctc
     1861 tggcatgttg aatagccgta accacagcgg cggtgcggga actggcgcaa taatgggttc
     1921 gaaaaacctg aaagcgattg cggttgaagg gacgaaaggg gtcaacattg ccgatcgtca
     1981 ggagatgaag cgtctcaatg attacatgat gactgaactt attggtgcga ataacaacca
     2041 tgtcgtgcca agtacgccac aatcgtgggc agagtattca gatcccaagt cacgctggac
     2101 agcacgtaaa gggctgtttt ggggcgcggc tgaaggtggt ccgattgaaa cgggtgaaat
     2161 tccgccaggc aatcagaata cggtcggctt tcgtacctat aaatccgttt ttgacttagg
     2221 accggcggca gagaaataca cagtaaaaat gagcggctgc cactcttgcc cgatccgttg
     2281 tatgacccaa atgaatattc ctcgggtgaa agagtttggc gtgcccagca caggtggtaa
     2341 cacttgtgta gcaaactttg tccataccac catcttcccg aacgggccga aagattttga
     2401 agataaagac gatggtcgtg tgattggtaa cctggttggt ctgaatttgt tcgatgacta
     2461 cggcctatgg tgtaactacg ggcagttgca tcgcgacttt acttattgtt acagcaaagg
     2521 tgtgttcaag cgtgttctgc cagctgaaga gtatgcagaa attcgctggg atcaactgga
     2581 agcgggtgac gttaacttca ttaaagattt ttactaccgt ctggcgcatc gtgtgggtga
     2641 gctgagtcac ctggctgatg gttcatatgc catcgcagaa cgctggaatt tgggtaaaga
     2701 gtactggggc tacgcgaaaa ataaactctg gtcgccgttt ggctatccgg ttcaccatgc
     2761 caatgaagcg tcagcgcagg tcggttccat tgttaactgt atgttcaacc gtgactgcat
     2821 gacgcatacc catatcaact ttattggttc cggcttgcca ttgaaactgc aacgtgaagt
     2881 ggcgaaagaa ctttttggtt ctgaagatgc ttacgatgaa accaaaaact acacgccaat
     2941 caatgacgca aaaattaaat atgcaaagtg gtcgctgttg cgggtctgtt tgcataacgc
     3001 cgtcactctg tgcaactggg tctggccaat gaccgtttcg ccgctgaaaa gccgtaatta
     3061 ccggggcgat ctggcgcttt aagccaaatt cttcaaagcg atcaccggcg aagagatgac
     3121 tcaggaaaaa ttagatttag ctgcagagcg tatctttacg ttgcatcgtg cctacacggt
     3181 aaaaccgatg caaaccaaag atatgcgtaa tgaacacgat cttatctgtt cctgggtttt
     3241 cgacaaggat ccgcagatcc cggtctttac tgaaggtact gacaaaatgg atcgtgacga
     3301 tatgcatgct tcgctcacga tgttctacaa agaaatgggc tgggacccac agcttggttg
     3361 tccaacccgc gaaacattgc agcgtctggg gctggaagat attgccgccg atctggcagc
     3421 acacaatcta ctgcctgcgt aaggaatggg taagatgaac catcaggatg aattgccgtt
     3481 agcgaaagtc agcgaagttg acgaggcgaa gcggcaatgg ttgcagggga tgcgtcaccc
     3541 cgttgatacg gtaaccgaac cagaacctgc tgaaatcctg gctgagttca ttcgccagca
     3601 ttctgcggct gggcagctgg tggcgcgcac cgtgtttctc tcgccaccct attcggttgc
     3661 agaagaggaa ctgtcagttt tgctggaaag cattaagcag aacggagact acgcggatat
     3721 cgcttgtatg acgggaagtc aggatgatta ctattactca acgcaggcca tgagtgaaaa
     3781 ctacgcggcg atgtctttgc aagttgtgga gcaggacatt tgtcgcgcca ttgcacacgc
     3841 cgttcgtttt gagtgccaga cgtatcctcg tccttacaaa gtcgccatgc tgatgcaagc
     3901 gccatattac ttccaggaag cgcagattga agccgcgatc gccgcgatgg atgttgcacc
     3961 tgaatatgcc gatatacggc aggtggagtc atcgacggcg gtgttgtact tgtttagcga
     4021 gcgatttatg acctatggca aggcttatgg cttatgcgaa tggttcgagg tagagcagtt
     4081 tcagaacccc tgaaatttca gggagatgtc atggataggg tggacagttg cagccactgc
     4141 attaggggat aatcagatgt cctttactcg acgcaaattt gttctgggga tgggaacagt
     4201 aatctttttt actggttctg cgtcttctct gttagcgaac acgaggcaag aaaaggaagt
     4261 tcggtatgcc atgattcatg acgagtcacg gtgcaatggc tgtaatattt gcgcccgcgc
     4321 atgccgtaaa actaaccacg tccccgctca gggaagccgt ttatcgatag cacacattcc
     4381 agtcaccgat aacgacaacg aaacgcagta tcactttttt cgccagtcat gtcagcactg
     4441 cgaagatgct ccgtgcatcg acgtatgccc gacgggggca tcgtggcgtg atgagcaggg
     4501 gatcgtgcgg gtagaaaaat cgcagtgcat cggctgcagt tattgcattg gcgcttgtcc
     4561 gtaccaggtt cgctacctta accctgtcac taaagtggca gataaatgtg atttctgcgc
     4621 tgagtcccga ctggcaaaag gctttccgcc catttgcgtc agcgcctgtc cggaacacgc
     4681 attgatcttt ggtcgcgaag atagcccaga aattcaggcg tggttgcagg ataataaata
     4741 ctatcaatat cagctacctg gtgccggaaa accgcatctg tatcgccggt tcggtcaaca
     4801 tttgattaaa aaggaaaatg tatgaacccg tcgcaacatg ctgaacagtt tcagagccag
     4861 ttagcgaact atgtgccaca gttcactccc gaattttggc cggtgtggtt gatcattgcc
     4921 ggagtattgc tggttgggat gtggttggtg ctggggctgc acgccttgct tcgtgctcgt
     4981 ggcgtgaaga aatcagccac cgatcatggt gagaaggttt atctttacag caaagcggtc
     5041 agattatggc actggtcgaa tgcgttactc tttgtattgt tgctggcaag tgggctgata
     5101 aatcactttg cgatggtggg cgcaactgcg gttaaaagtc tggttgcggt gcatgaagtt
     5161 tgcggatttt tgttactggc atgctggctc ggctttgtgc tgatcaatgc cgttggggat
     5221 aatggtcacc actatcgcat tcgtcgtcag gggtggctgg aacgagcggc aaaacaaacg
     5281 cgattttatt tgtttggcat tatgcagggg gaagaacatc ctttcccggc aacaacacag
     5341 tctaaattta atcccttaca gcaggtcgcc tatgttggtg tcatgtatgg attgctgccg
     5401 ttgttactat tgacggggct gttgtgtctc tatccgcaag ccgtgggaga tgtgtttcct
     5461 ggcgtaagat actggttatt gcaggcgcat tttgctctgg catttataag cctctttttt
     5521 atcttcggtc atctttatct ttgcaccacg gggcgtacgc cacacgaaac ctttaaaagc
     5581 atggtcgatg gctatcaccg gcactaacga atgatcatta cacgagcaga cctgcgagaa
     5641 tggcgtatcg gagcggtgat gtatcgctgg ttcctgcgcc atttcccacg cggcggtagt
     5701 tatgccgata ttcgtcatgc actgattgag gaaggatata cggactgggc ggagagtctg
     5761 gttgagtatg cgtggaaaaa atggctggcg gatgaaaact tcgcgcatca ggaagtttcc
     5821 tctatgcaaa aactggccac agaccccgga gagataccat tttgcagtca gtttgcccgc
     5881 agcgacgatc atgctcgcat cggctgttgt gaggataacg cccgaatcgc aaccgctggg
     5941 tatgccgcgc aaattgccag tatgggttat agcgtgcgga ttggaagtgt cggctttaac
     6001 agtcacattg gcagctccgg tgaacgcgca cgggttgctg taaccggtaa ttcttcgcgg
     6061 ataagcagcg ctggagatag tagtcggatt gcgaataccg gaatgcgagt acgcgtttgt
     6121 acattgggtg aacggtgtca tgtcgccagt aatggtgacc tggtgcaaat cgccagcttt
     6181 ggcgcaaacg ccagaattgc caacagcggc gacaatgtac acatcattgc cagcggtgaa
     6241 aattcaacgg tagtgagcac gggcgtggtc gactcaatta ttctcgggct agggggaagc
     6301 gccgcactgg catatcatga cggcgaaaga gttcgcttcg ccgtcgccat tgaaggtgaa
     6361 aacaatattc gaaccggagt ccgctatcgt cttaatgaac agcatcagtt tgttgagtgc
     6421 tgatagcttt acctttaatc aaacgaaaga cgccgacgag cacgggatgc aggctttacg
     6481 accgcccgag ccatcgcctc accaatttct gcacatgccg taagtccctg aacgaaaggc
     6541 tggtcgtgca tcaaccaggg caacgcgccg aacgctacgc gtccacgaat atcttcgggt
     6601 tgctgtaacg tataatcttc gccaacatca gggatttcat cccctgtttt ctgtaattgc
     6661 tcgcgtagcc cagggaaagg aatatctttc actttaagcg gacgctgtcc gcgggcatca
     6721 ataaaaacgt caaacgagta gctgttgtct tccgttttca ggacggtgcg cgactcatta
     6781 atttccattt cgtagtcttc accgagggcg agaatatgaa tgatccccgc ttcacgtaag
     6841 gccagtagtc gacgaataga ctctgaaggg attgccgcat aattatcgat gaaaaccctg
     6901 gcaaggcctt tactgaaccg tttatggtcg tgttcattca gatgaggaac aatttcctgt
     6961 acggcttcat gcaggcgcag aatgacataa cgccagggaa cagtatgttt ttctcgttta
     7021 ttgcgttcga cttcctggag atttttttct gcccagtcaa atgggtcgcg ttgtttgcgc
     7081 tcggcaaacc aggcttgagc aaaggaatcg acattcaggc tctctaaggc tatgcgttgg
     7141 ctccagtctg gatcagcaaa cttgatttcc tctactatca atctaaatac ccgatccaaa
     7201 aggccctctt cacctttttg aatctcagca tttaatgcct gatcggtgac gatgtgtaag
     7261 ggctcgtagg gaatagggca atagaaatcg gcttcgggta aaatacccgt gcgcgacatc
     7321 aacgtaatat ttagcttttc acttgcgtta tcgcggtgaa aaacgacgtg ttgtttatca
     7381 tcttcaatga acgaaccatg ctgaatagcc actgccattg ccgcatccag tccgctcaag
     7441 gatgttccca taatacccac gttacacgca tcgacctttg cttccatcag gcctgaccac
     7501 gggctgggaa aatacgttct ggttgcttct tcttcatcag gccagacgtg acccgtggca
     7561 atcaccgcta aatcaaacgt ctcgctgggt aaatcctgat ttgtagcgag catgacgcca
     7621 gcatttgtaa tttgcagatc ggtaacctgg catgattcat aaacagccac tgcaaatttt
     7681 tgctgtcgtg cctggtctac taatcgtaaa aattgatcgc ggaaatattc gcccagcaga
     7741 attcgcggta aaaactgacg atcgtgcaag gtttcttttt taacgccata acgctggaga
     7801 tggctggctt cttgcttttg tagccattcg agatacgtac aattaatcgg cggtatttca
     7861 atactggcaa tatttgccag catcattttt gagttttcct catcactgta tggcatcccg
     7921 acaccggcct cgtcagcctg ctcgaagata gaaatagaaa gtggagtttg ttgctgtaga
     7981 agcgagaata aggtgtagat ccccgtaggc ccggcaccca caatagcaat ttttttcatt
     8041 gttacttacc tgtacgcatt ctttcgaatt tagtgttatc agataagttt ggttgagatt
     8101 tttagaattt gagaaatttc agacagggat gaaaagggcg ctaaccgccc ttgattaaat
     8161 tctgtcaggc gagttttgcc tgattgattt gactaagtgg ctcattgaca tcgaaaactt
     8221 tggcgacaac ttcctcaacg ccgagatttt tcagtcgggc agtatgtttt tccagatagg
     8281 caagggcgct tttttcatca gtgaaaaggt agatcccacc ggcttcgtgg ttcttttcac
     8341 tttctgtcca taccttccac agaaaaccag gttcctgatt aatcgactca gcaagtggtt
     8401 taagctgctc agccattgcg tcgccaaatg ggccattaaa agcaaaatga agttgtaaca
     8461 aagttgccat agcgattctc cttctgataa cgcatacgaa gacagtgtaa atcttttaac
     8521 gtattggata cagagaagaa acagagggaa aaaggaaaag accagattaa gaaaatctgg
     8581 tgcgtccgag tggactcgaa ccaccgaccc ccaccatgtc aaggtggtgc tctaaccaac
     8641 tgagctacgg acgcaggatg gtgcgttcaa ttggactcga accaacgacc cccaccatgt
     8701 caaggtggtg ctctaaccaa ctgagctatg aacgcaacgt tgtaggtgac aacggggacg
     8761 aatattagcg gcagagtgga aaggtggcaa gaggcaaaac gtaattttct gcgctatttc
     8821 gaccgtttgc agagctttta agcaaattgg ctatattttg ttgatttgca agggcgattt
     8881 ttattcagga tcgcatttac atctgataca acccaataat tcgtgtatca gagtttaaac
     8941 agagttgttt gccaccatct caaatctgtc gtggataaat cctccgtagc ctttacactt
     9001 aggtagcaat aaaggcacac agatgcagca aaggaataaa tcatgggatc tgatgcgaaa
     9061 aacttgatga gcgacgggaa tgtgcaaatt gttaagaccg gcgaggtcct tggcgcgacg
     9121 caacttactg aaggcgagtt gattgttgaa gctggcggaa gagccgaaaa taccgtggtc
     9181 acgggggctg gctggttgaa agtggcaacc ggtgggatcg ccaaatgcac acagtacggt
     9241 aacaatggca cgctatcggt cagcgacggt gccattgcca cagatattgt tcagtccgag
     9301 ggaggcgcaa ttagtctctc tacgctcgct acggttaatg gccgccatcc cgaaggtgaa
     9361 ttcagcgttg atcagggtta tgcctgcggt ttgttgctgg aaaatggcgg taacctgcgt
     9421 gtactggaag gacatcgcgc ggaaaaaatc attctcgatc aagagggcgg cctgttggtt
     9481 aatgggacaa cctcagcggt cgtggtagat gaaggtggtg aattgttggt gtatccaagt
     9541 ggagaagcca gcaattgtga gattaatcag ggcggcgttt ttatgctggc cgggaaagcc
     9601 agtgatacgt tgcttgctgg tggcaccatg aataatctcg gtggtgaaga ctctgacact
     9661 attgttgaga atggatccat ttatcgtctg gggacggatg gccttcagct ctacagttcc
     9721 ggtaagacgc aaaacctgtc ggtgaatgtg ggggggcggg ctgaagtgca tgccggtacg
     9781 ctggaaaatg cggtaataca aggtggaaca gtgatcctgt tgtcacccac cagcgcggac
     9841 gaaaattttg tcgtagagga agatcgcgca ccggttgaac tgaccgggag tgttgcatta
     9901 ctggacggcg cttcaatgat tattggttat ggcgcagagc tgcaacaatc aacgattact
     9961 gtacagcagg gcggtgtgtt gattctcgac ggcagtacgg taaaaggtga cagcgtcact
    10021 ttcattgttg gtaacatcaa tctgaatggc ggaaaactgt ggctgatcac tggtgcggca
    10081 acgcatgtgc aactgaaagt gaaacgcctg cgcggagagg gagcgatttg cctgcaaacc
    10141 agtgcgaaag aaatttcacc tgacttcatc aatgtgaaag gggaagttaa cggggatata
    10201 cgcgttgaga taacagatgc cagtcggcaa actctgtgta acgctctgaa attacagcca
    10261 gacgaagacg ggattggcgc aacgctccag cctgcgtaaa aacacaggcg caacccgtga
    10321 aggttgcgcc ttgtctttat tagcgggacg ctcgttgcag aatgatggct gacggcatac
    10381 gttgcaggaa ccgcatacgc agcatcatca taatggctgc cgatgtcagg ccgataataa
    10441 agcctatcca gaagcctgct ggccccatag gttcaacgac cagatcggtc agtgccagaa
    10501 tatagccgct tggcaagccc agcacccagt aagctgtaaa ggtgatatag aaaatggaac
    10561 gcgtatcttt ataaccacgc aaaatcccac tgccaatcac ctggattgag tcagaaatct
    10621 gatataccgc cgccagcaac atcaaatgcg cagccagcgt tacaacctcg ggattgtcgt
    10681 tgtacaacag ggcgatttgc tcccgcagtg aaaccgtgaa aatggccgtc agggttgcca
    10741 tacagacacc caccataagc ccggtccgcg cagcggtttg cgcatccagc gttgagccct
    10801 gacccagacg ataacctacg cggatagtta ccgctgccgc cagcgacatt ggaagcacaa
    10861 acatcagtga actaaagttc agggcaatct ggtgtcctgc gacatcaaca ataccgagcg
    10921 gagacactaa cagagccacg acggcaaaca gtgtcacttc aaagaacagt gccagcgcaa
    10981 tcggcaaacc gagttgaatc agtcgtttca taaccgcagg ctcgggctta gcggtgcctt
    11041 tttcgttacg aatatcgcgc atggagcggg cgcgtttaat gtaagaaacc atggcaagga
    11101 acatgaccca atacaccgcc gcagtagcca cgccacaacc aacgccaccg agctcaggca
    11161 taccgaaatg accataaata aagatatagt tcaccgggat gttcaccagc aggccgataa
    11221 agcccattac catacccggc ttggtttttg ccagaccttc acattggtta cgggcaacct
    11281 ggaagaacag atatcccggc gcgccccaca acaacgcacg cagataaccc acggctttgt
    11341 ccgccagagc cggatcgatg ttttccatgt agcggataat gtaacctgca ttccacagca
    11401 ccagcataat gagaacggaa acaaaacctg ccagccagaa accttgtcgc acctgatgcg
    11461 caatgcgctc acgtcgaccg gaaccattta attgcgcgat aaccggcgtt aatgccagca
    11521 gcagtccgtg accaaagagg atcgccggaa gccagataga agtaccgata gcgaccgccg
    11581 ccatgtcggt ggcactatag ccgcccgcca tcacggtatc gacaaaaccc atcgcagttt
    11641 gggcgatttg cgcgagaatc accgggattg ctaatgctaa taacagacgc gcttcactga
    11701 tatacttctg cacgtgaaca ccttttattt gtagttatat gagagactaa aaagccgcct
    11761 gaatgggcag caaaaagaag aagcagggga aattcagtct attgtagcga ggatttatta
    11821 attctccagt gaaaaaatag ccattcttac cccttcactg gtaagggcgt tttccacctg
    11881 ctattgtgct gaacagaatg tcttaactga tttcaggagt tgtaagtatg tttacgggga
    11941 ttgtacaggg caccgcaaaa ctggtgtcga ttgacgagaa accaaatttt cgtacgcatg
    12001 tggtggagtt acccgaccac atgctggacg gcctggaaac cggtgcttcc gtggcgcata
    12061 acggttgctg cctgaccgtg acggaaatta acggcaacca tgtcagtttt gacctgatga
    12121 aagaaacgtt acgcattacc aatcttggcg atttaaaagt gggggattgg gtaaacgttg
    12181 agcgtgcggc gaaattcagt gatgaaattg gcggacactt aatgtcaggt catattatga
    12241 ccaccgctga agtggcgaaa atattaacct cggaaaataa tcgccagatc tggtttaaag
    12301 tccaggatag tcagttgatg aaatatatcc tgtacaaagg atttattggc atcgacggta
    12361 ttagcctgac cgtcggcgaa gtcacgccaa cgcgtttttg cgtccattta attccggaaa
    12421 cactggaacg cacgactctt gggaagaaaa aacttggcgc acgcgtcaac attgaaatcg
    12481 atccacaaac tcaggcagtg gtagatacgg tagaacgtgt gctggcggca cgagaaaatg
    12541 ccatgaatca accaggtaca gaagcctgat ctgaactcgc ccccgaagag ggggcgatag
    12601 aatagccttt agcgagcgac tcgaaggccg ttttcaacac cgcgtgagaa caccacctgc
    12661 cagagctgaa tatcacgggc gcggaaagca cctgcgcagg cattcagata ataggtaaac
    12721 atccgtttaa agcgttcact gtagttatcc gcaatttctg gccatgcggc gaggaatcgt
    12781 tcataccacg ccatcaacgt agtatcgtaa tcagcaccga agttatgcca gtcttccatc
    12841 acaaagtggg gttcgctgga ttgagcaatc tggcgtacag agggcaggca accgttcgga
    12901 aaaatatatt tattaatcca gggatcaaca ttcagatcgg tttttttcga accgatagta
    12961 tggagcagga atataccttc cggtttcaaa ttacgatcca ccaccgcaaa ataggtatcg
    13021 taatttttcg gtccgacgtg ctcgaacatc cccacagaaa caatacgatc aaactggtcg
    13081 ttcaggtcac gataatcttg cagcaaaatg gtgacatcca ggcctttaca gcgttcctga
    13141 gccatttttt gctgttcggc agaaatggtg acgccgacca cgcttacgtc atagttagat
    13201 gccatgtagt gcgctagtcc gccccagccg cagccaatat ccagtacgcg catccctggt
    13261 tttaactgca atttttcaca aatcattttg agcttcgcct gctgggcaga ttccagatta
    13321 tcggcatctt tccagtaagc gcaggaatat tgcatgaagg gatcaagcat gcggctgaac
    13381 aagtcattac ccaaatcgta atgctccttg ccgactatcc aggcacgttt tttactctgc
    13441 agattgaaga gacgagcgcc ggcaatacgc agcgtgtctt tgaaatgatg ggggagttgg
    13501 ttctcgagac ctgcgcgtaa gactttgcta aaaaacatat ccagtcggtc acattcccac
    13561 cagccatcca tataactttc gcctaacccc aaagagcctt cctgcagaac gcgtttaaaa
    13621 aaatcggggt ttttcacacg aatatccgcc ggggcagaac cgttaatggc tataccggca
    13681 cggctaagta attcgttggc gatacggtac cagttgtcat ccggtacact gacttcttct
    13741 atacacgatg aactcatagt ttctccatca ctggtcggtg atcagaacct taaaacagcg
    13801 tagacgcttt ttgggctttg tgagaaatct cgcagaaaaa accgcgagtc tataatccga
    13861 cgtagaacag agggaaagga ggaacccttg ccgaaatgac catccatagt gagtcgggaa
    13921 cgatcctgtc cccgtaaaaa cataatattt atcgtagctt taacaactcg aaattagtat
    13981 aggccgctaa tttcaatcat acaacaaaat tttgttagca gcctaagtat aagtatatcg
    14041 atatagatca gtgtgattcg ctatgagcga cttcggcatt gccatgattc gggcagtcaa
    14101 cttcttcaca acgttgcatc atgtaaccca gcgcgaccag cactactgtt gataacatca
    14161 cgctggtggt ggtgagcaat ggcgtgctga tactaataag ccaggaaact accagacttg
    14221 cgaggaagca cagacccagt tgcagagtgt tctgcaacgc tgcggcgcga ccggttgcat
    14281 gtgggaaggg acgcagcgcc tgggcgacaa caatagggta gatcgcgcca ttggcaatcg
    14341 ccatcacaca gaatgggatc aggatttcga ccagcgacac atggctaatg aagcccgcag
    14401 cccaggtcgc aatgacgctg acagcaaaca gcaccagcaa ccacggtaat aactgcttgc
    14461 cttgccattt ctgcagtgcg gcgcgacagc cataaccacc aatcagaaac gcgatagttt
    14521 gcgggacata acttaaacca ataactgccg ggctgtagcc catttcacta aggatgaacg
    14581 gtgaaccggt cagccatgca aaaaaactgg ctgaacaggc tgcgtatatc agcacgttgc
    14641 cgcgataggt tttagaacgt agcaggtcgg taaaggtcag accatcctga ctattgttac
    14701 gggccttcgt cgtgggtttg agccagaaaa taggcagaat cagcaccacg gtaatggcaa
    14761 acagggtggc gaaaatcgcc tgccaggaaa aatggaccag cagccagctt cctaacaggg
    14821 gagccagtgc cggagacaga cccaccagcg gcatgatggt cgcaaaaata cggttgactt
    14881 tctgtgaagg ataataatct gtcactaatg cttgccagat aaccgccgcg gcgcagaccc
    14941 ccacagcctg tacaaaacgc aataccagca gcgtggcggc gttttctacc cacagcatcc
    15001 ccagactacc taacgcaaaa attgtcaggc cgattaataa caccggttta cgaccataac
    15061 ggtcggagag tggcccccac agaagctggg ctgcggcaaa accggcaagg aacagactaa
    15121 ggctggcact gacagcagac gcaggcgttt gcaggtcggc ctgtatggcg gcgaaagcag
    15181 gcagatacat atcggttgcc agaaaaccga gtacgctcaa acccgccagc cagactaaaa
    15241 atcttttccc aggttgcatg tatattctct aaatctctca attaaacacc gccgcaaagt
    15301 gtagggagtg aaattagtgt tgtgaaacgg taatatttga tgactggttt caaaaatttt
    15361 gcaggcagac tatgtggtca gaatattcac tcgaagttgt tgatgcggta gcgcgtaatg
    15421 gtagttttag cgctgcggca caggagctgc atcgtgttcc ttctgcggtc agctataccg
    15481 tgcgtcagct ggaagagtgg ctggcggtac cgctctttga acgacggcac cgtgatgtgg
    15541 agctgaccgc tgctggagcg tggtttctca aagaagggcg ctctgttgtc aaaaaaatgc
    15601 agatcacccg ccagcaatgt cagcagatag cgaacggctg gcgcggtcag ttagctatcg
    15661 cagtggataa tattgtcagg ccagaacgta cacggcagat gatcgttgat ttttatcgcc
    15721 attttgatga tgtcgaactt cttgtctttc aggaagtgtt caacggtgtc tgggatgcgc
    15781 tttccgacgg gcgcgtggaa ctggcaattg gcgcaacacg cgcgattccg gtaggcggtc
    15841 gttatgcctt ccgggatatg gggatgctaa gctggagttg cgttgttgcc agtcaccacc
    15901 cgctggcgtt gatggatggc ccgttcagcg atgatacgtt gcgcaactgg ccgtcgttgg
    15961 tgcgcgaaga cacctcgcga acgctaccta aacgtattac ctggttgctg gataaccaaa
    16021 aaagacttgt cgtacctgac tgggaatcat cggcaacctg tatctcggca ggattatgca
    16081 tagggatggt gccaacacat ttcgccaaac cgtggcttaa tgaggggaag tgggtagcac
    16141 ttgaactgga aaaccccttc ccggattcgg catgttgtct gacatggcag caaaatgata
    16201 tgtcgcctgc gttaacctgg ctgcttgagt atctcggcga tagtgagacg ctgaataagg
    16261 agtggctacg ggagccggaa gagactcccg caacgggtga ttaacgacga tagtcgcgga
    16321 acgggccgtc agccacggag cggcgttcaa tcaaacgcgg atgcacttca atggactgcg
    16381 gttcttcacg tttgttgacg atacgatcca acagcatgtt gaacgctgtt tcacccagcg
    16441 aatcttttgg ctgatggatc gtggtcagcg ccggcgtaaa atagcgcgcg ttgcgcacgt
    16501 tatcataacc gatcagcgaa acatcctgcg ggacgcgcag gcccatttca tcagcagcac
    16561 aaagtgcgcc cattgccatg atatcgccac cacagaagac ggcagtaggg cgatgcggct
    16621 gcgacaggat ttgctgcatg gcgcgataac cggattcagg ttcaaagtca ccctgcacaa
    16681 tccagctttc cggcaccttg atcatcgctt cttccatcgc cttcataaaa ccggcaaggc
    16741 ggcctgcgcc ggtgttacgt tccagcgggc cggggatgac gccgatttcg cggtgaccgc
    16801 gttcaatcag ataacgcccg gccatgtagc cgccttcgaa cgcgttatca atgaccgcat
    16861 cggtgaagtc agcttttgct tcaccccagt ccatcaccac cattgggata tggcgatact
    16921 cttccagcat cgccagcaac ggctctgggt actcagaaca catcaccagc agaccatcga
    16981 cgcgtttttg cgccatcatc gacagataag cccgctgttt ctcaagattg ttccacgcat
    17041 tacccagaat cagggtgtaa cctttctgga agcaattttt ttcaactgct tcaatgatct
    17101 cggcaaaata ggccgcttcg ctgctggtcg ccagcaaacc gatagacttg gtgtggttaa
    17161 ccttcaggct acgcgccacc gcgctagggg agtagtgtaa ttctttaatc gctgcccaca
    17221 cggcgttgcg cgtttcttca gcgacgaaac gtgttttgtt gatcacgtgt gacacagttg
    17281 tagtggaaac gtttgctcgt ttcgctacat cttttattgt tgccattcca tttcactcca
    17341 gaccctaact tcagctcctg caaaatcgca aggtaaacgt ttgcctttac acaccttacg
    17401 cacaacaaaa gtgttgcggt acgccggaaa aacgacacgg agcgtcagga aggggtcaat
    17461 ggccagtacg caaatagtgt aagcgtggaa ttttggccga tcttgacgaa aaggggaagt
    17521 ggtaaaaaca cttatcatta taaatgacgg gagatttttt catcacagtg tgtaaaaatg
    17581 cgtaatatca ctttttgtgt ggtaataaaa aaggagaaaa cttgatgagc accgacctta
    17641 aattttcact ggtaacaacg attatcgtcc tcggtttgat cgtagccgtg ggtttgactg
    17701 ccgcgctgca ctgatttcct ctcgagggag cgcgttctcc ctcgcatctc cttcctgttt
    17761 gttagcaaaa tggcaaaagt gttttctctt tttgttattt tcattttttt gtgattgctg
    17821 tcatgctttc gccactttaa tgaatatcgc gtaatgacgc gaagaggtgg agataaggca
    17881 tgaaaattaa ctatccgttg ctggcgctgg cgattggcgc gtttggtatc gggacaacgg
    17941 agttctcgcc aatgggcttg ttgcccgtca ttgcgcgcgg tgtggatgtc tcgattcccg
    18001 ctgccggaat gttaatcagt gcctatgcag ttggcgtaat ggtgggcgcg ccgctgatga
    18061 cgcttctact ttctcatcgt gcccgccgca gtgcgttgat tttcctgatg gcaattttca
    18121 cgctaggcaa cgttctttcc gccatcgcgc cggattatat gaccctgatg ctttcacgta
    18181 ttttgaccag cctgaatcac ggagcatttt ttggtttggg ttcagtcgtg gccgcaagcg
    18241 tggtgccaaa acataaacag gccagcgcag ttgccactat gtttatgggg ttaaccctgg
    18301 caaatatcgg tggcgtgccg gcggcgacct ggttgggtga aaccatcggc tggcggatgt
    18361 catttctggc aacggcgggg ctgggagtga tttcaatggt aagtctgttc ttctcattac
    18421 ctaaaggtgg tacaggggca cgacctgaag tgaaaaaaga gctggcggta ttaatgcgtc
    18481 cgcaggtgct gtctgcattg ctgacgacgg tactgggagc tggtgcaatg tttaccctct
    18541 acacctatat ctctccggta ctgcaaagta ttacccacgc aacaccggtg ttcgtcacgg
    18601 caatgctggt gctgattggt gtcggattct ctatcggtaa ctatctcggc ggcaaactgg
    18661 cagatcgttc agttaacggc acgttgaaag gctttttgtt gctgctgatg gtgattatgc
    18721 tggcaatccc gttcctggcc cgcaatgagt tcggcgcagc tattagcatg gtggtgtggg
    18781 gcgcagcaac ctttgcggtt gtacctccgt tacagatgcg cgtgatgcat gtcgccagtg
    18841 aagcgccagg tctgtcttca tcagtcaata ttggtgcctt taatcttgga aatgcgctgg
    18901 gagcagctgc tggtggtgcg gtaatttccg ctgggctggg atacagcttt gtgccggtga
    18961 cgggggcgat tgtcgcggga ctggcattat tgctggtgtt tatgtcagcc agaaaacaac
    19021 ctgaaacagt ttgcgttgct aacagctaat taaacaaaaa cgcagaagga taaccttctg
    19081 cgttacagtt gctgacaacg tgcgatttat cttgccgaat gcgctgttaa cgcctcatcc
    19141 gcgttggcat ggtaagcgaa gcgcatcagg caatgctgca tttgccatca gttattatgc
    19201 agcgagattt ttcgctacga attcccagtt caccagcgcc cagaagtgct ccagatagcc
    19261 aggacgtgca ttgcgatagt cgatgtaata agcgtgttcc cagacatcaa cggtcagcag
    19321 cggagtcgca tcggtggtca gcggagtacc cgcgttagag gttgaaacga tagccagttt
    19381 gccatcgctg tttttcacca gccaggtcca gccagaacca aagtttttga tcgctgcatc
    19441 agtaaactgc gctttgaaat cggcaaagct gccaaaagat gcggcgatag cttcagcgac
    19501 ttttccagtc ggttcgccac cggcgttcgg tgccaggcag ttccagtaga aagtatggtt
    19561 ccagacctga gctgcgttgt tgaatacgcc accttcagag ctgcgaataa tctcttccag
    19621 tgatttacct tcaaacgcgg tacctttaat caggttgttc aggttagtga cataagtctg
    19681 atgatgcttg ccgtagtgat actcgatagt ttccgcagaa atgtgcggtg ccagagcatc
    19741 tttagcatat ggtagtgcag gtaattcgaa tgacattgct actctccttt attattaatt
    19801 tgcatacgta caatagcctt attgtgcgta tggatagggt agcaaattaa aaggtgagac
    19861 aaaagatgaa cttaccctgt tgccgtaaca acagggtaaa gttttagcga agtgtttttg
    19921 gggtcattac ccgacgagcg ccaacatagt ggcgctgcca gtagtcttca ctgagagaag
    19981 tgatttgaat ttcctgacct gtgcgcggtg actgaataaa tttgccgttg ccgacataca
    20041 cgccgacatg atcggctgta ccgcgtccct gagtacggaa aaagaccagg tcgccgtttt
    20101 tcagctcact acgttcgata ggtcctgcat cacgcaggtg atacatttca ttcgccgtac
    20161 gcggaatacg aattttcacc aaatctttat aagcgtaata aaccaggccg ctgcaatcaa
    20221 aaccggtatg cggtgagctg ccgccccaac gatatggctt accaatttgc tgcatcagtt
    20281 tattcattgc cactttcgtg gctttttgca ccttcgcttt gtgcgcgtcg gcaagggttc
    20341 ctgacgcggc atttttgact ttcacacaat gcgatttata gcccttacgt ttggtacact
    20401 tttcggtaaa gctggctgag gccgttttat tgacggcgtg tttgctgcga gttttggcgt
    20461 ttttagaagc ggtaatggaa gattttttaa cagaagaagc ggttttgctt ttggtcatac
    20521 ttttactggc ggcttttttc gctgttttct ggctggcttt ctgggtcttt ttggtggttg
    20581 ccgtgctttt ctttttatct gcttttttgg tgatatgagt ggtagcagaa ctctccctgg
    20641 cttgctttga agcatgggcc ataggcgtta aaggcagggt ggtaaaaagt aaagcacaga
    20701 gcgtgatcga aatacggttt atccgcgcca ctgagcgtcc ctctgttaag gcaggtctat
    20761 catgcctgcg tctgatttac aaaacttgat gattctaatg catagaagaa gagaaagtta
    20821 atagctttta ttgttaaaaa atacgtttcg ttggcgagcg caaaaaaatg cagcagtgaa
    20881 agctaaaaat ggttataaat acgccagttt gccgcgcatc agagaagaaa cgaatatttg
    20941 aaatgcaaca aatagagcaa agggataaaa tagcaaaagc gtgatgaaaa tgttattttc
    21001 cgatgccgca ctgacccgct acaatgtcag attactgccg taaagaagtt tgaggaagca
    21061 agacaatgag caccactatc gaaaaaatcc aacgccagat tgctgaaaac ccgatcctgc
    21121 tgtacatgaa aggttcaccg aaactgccga gctgcggttt ctctgcccag gcagtccagg
    21181 cgcttgccgc atgtggcgaa cgttttgcct atgttgatat tctgcagaat ccggacattc
    21241 gtgcggaact gccgaaatat gctaactggc cgaccttccc gcaactgtgg gttgacggcg
    21301 agctggtcgg cggttgtgat atcgtgatcg aaatgtatca gcgtggcgaa ctgcagcagc
    21361 tgatcaaaga aactgccgct aaatacaagt ctgaagagcc ggacgcggaa taatccgtcc
    21421 tgcaatactg acggatgcga acgggcatcc gtcagtcctt tatccggtgc gtcgtaaaac
    21481 ctcgtccttc agggcgggga tataaggcgc ggttttccac ctgaccaggt gtttgctgtt
    21541 gttcaatgta ctggcggatg acggatattg gcgcaccttc gcagctactg gcaaaatagc
    21601 caggactcca caaaacgcct ttgtagtaat acctgactgc aatatctggt cgatctcgtc
    21661 gcagtaacct attcgatatg cctttgaggc tgtttaccag actggatatc gccagtttgg
    21721 gaggatagtt aattaacaaa tggacgtgat ctggttcgcc atccatttca accagttcag
    21781 ctgcaaaatc agcacataca tttgaaaagt aagtgcgtag tttttctgtc gtgtcatggt
    21841 caaaaatctg gcgtcggtat ttggtgacaa agaccaggtg aacatgcatc aggaaaacac
    21901 aatgccttcc acgccggata tcggtttctt ttttcacaga ccaaagtatc atttgacctg
    21961 tgaaacgatt acaggcattt aaattccagt taagaccagg tggtcaacag gagcgtgaaa
    22021 tgaggcgctt cgcaggcgca tgtcgtttcg ttttcaatcg tgctctggcg cttcagaatg
    22081 agaatcatga ggccggtaat aaatacatcc cttacgggaa aatggcttcc tggctggttg
    22141 agtggaaaaa cgccactgaa acgcaatggc ttaaagattc gccctcacag ccattgcaac
    22201 agtcactgaa agaccttgag cgggcttaca aaaacttctt ccagaagcgg gctgcttttc
    22261 cccgattcaa aaagcgggga cagaatgctg cattccgcta cccgcaggac gttaagctcg
    22321 atcaggaaaa cagccgtatt tttctgccga aacttggctg gatgcgctac cggaatagcc
    22381 gtcaggtcac gggagttgtg aaaaatgtca ctgtcagcca gtcctgcggt acgtggtaca
    22441 tcagtattca gacagaaagt gaagtatcaa ctccggttca cccttcagca tcaatgatcg
    22501 ggctggatgc tggcgtggct aaactcgcca cgctgtcaga tggcacagtc tttgagcctg
    22561 taaacagttt tcaaaaaaac cagaagacgc tggcgagact tcagcgacag ttaagccgga
    22621 aggtcaaatt cagcaacaac tggcagaagc agaaacgcaa aatacagcga ctgcattcct
    22681 gtatcgcaaa tatccgcagg gactaccttc acaaagtcac aacgaccgtc agcaaaaacc
    22741 acgcaatgat agtcattgag gatttgaagg tcagcaacat gtcaaagtca gcagcgggta
    22801 cggtcagcca gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg
    22861 atcagggctg gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg agtggcggtc
    22921 aggtgcttgc tgttccgcca gcgtacacaa gccagcgttg cgc
//
LOCUS       DAJVRP010000063        22616 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVRP010000063 DAJVRP010000000
VERSION     DAJVRP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.063"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..434)
                     /gene="pgrR"
                     /locus_tag="OQN12_002526"
     CDS             complement(<1..434)
                     /gene="pgrR"
                     /locus_tag="OQN12_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1654807.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
     gene            572..1492
                     /locus_tag="OQN12_002527"
     CDS             572..1492
                     /locus_tag="OQN12_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1654808.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1492..2421
                     /locus_tag="OQN12_002528"
                     /pseudo
     CDS             1492..2421
                     /locus_tag="OQN12_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            2439..3077
                     /locus_tag="OQN12_002529"
     CDS             2439..3077
                     /locus_tag="OQN12_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1654809.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            3368..4096
                     /gene="mpaA"
                     /locus_tag="OQN12_002530"
     CDS             3368..4096
                     /gene="mpaA"
                     /locus_tag="OQN12_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1654810.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4071..5030)
                     /gene="ycjG"
                     /locus_tag="OQN12_002531"
     CDS             complement(4071..5030)
                     /gene="ycjG"
                     /locus_tag="OQN12_002531"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS1654811.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5149..5655
                     /gene="tpx"
                     /locus_tag="OQN12_002532"
     CDS             5149..5655
                     /gene="tpx"
                     /locus_tag="OQN12_002532"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1654812.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5699..7240)
                     /gene="tyrR"
                     /locus_tag="OQN12_002533"
     CDS             complement(5699..7240)
                     /gene="tyrR"
                     /locus_tag="OQN12_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1654813.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(7388..8449)
                     /locus_tag="OQN12_002534"
     CDS             complement(7388..8449)
                     /locus_tag="OQN12_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1654814.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(8446..9843)
                     /locus_tag="OQN12_002535"
     CDS             complement(8446..9843)
                     /locus_tag="OQN12_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1654815.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            9999..10997
                     /locus_tag="OQN12_002536"
     CDS             9999..10997
                     /locus_tag="OQN12_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1654816.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYARV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11108..11980)
                     /gene="ompG"
                     /locus_tag="OQN12_002537"
                     /pseudo
     CDS             complement(11108..11980)
                     /gene="ompG"
                     /locus_tag="OQN12_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(12053..13134)
                     /locus_tag="OQN12_002538"
                     /pseudo
     CDS             complement(12053..13134)
                     /locus_tag="OQN12_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(13148..13807)
                     /gene="pgmB"
                     /locus_tag="OQN12_002539"
     CDS             complement(13148..13807)
                     /gene="pgmB"
                     /locus_tag="OQN12_002539"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCS1654817.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(13804..16071)
                     /gene="ycjT"
                     /locus_tag="OQN12_002540"
     CDS             complement(13804..16071)
                     /gene="ycjT"
                     /locus_tag="OQN12_002540"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCS1654818.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16068..16691)
                     /locus_tag="OQN12_002541"
     CDS             complement(16068..16691)
                     /locus_tag="OQN12_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1654819.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(16688..17110)
                     /locus_tag="OQN12_002542"
     CDS             complement(16688..17110)
                     /locus_tag="OQN12_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1654820.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(17132..17907)
                     /locus_tag="OQN12_002543"
                     /pseudo
     CDS             complement(17132..17907)
                     /locus_tag="OQN12_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(17925..18977)
                     /locus_tag="OQN12_002544"
     CDS             complement(17925..18977)
                     /locus_tag="OQN12_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCS1654821.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19008..19850)
                     /locus_tag="OQN12_002545"
     CDS             complement(19008..19850)
                     /locus_tag="OQN12_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCS1654822.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19837..20718)
                     /locus_tag="OQN12_002546"
     CDS             complement(19837..20718)
                     /locus_tag="OQN12_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCS1654823.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20739..22022)
                     /locus_tag="OQN12_002547"
                     /pseudo
     CDS             complement(20739..22022)
                     /locus_tag="OQN12_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(22036..>22616)
                     /gene="ycjM"
                     /locus_tag="OQN12_002548"
     CDS             complement(22036..>22616)
                     /gene="ycjM"
                     /locus_tag="OQN12_002548"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCS1654824.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5573 a         6086 c         5507 g         5450 t
ORIGIN      
        1 acgcctgcat caaaacgttc agaaacgaca tcggtcaaac cataatcaat ggtaagctga
       61 atatcaattt caggatgcga tttcaggaat gtgcgcatgg ctggtaacaa tatcgttttt
      121 gctgcatgtt ctacagtagt aatacgtatt gtcccggatg ggcggttctg cagatcgctc
      181 agggatgtca gggcagaatc tatgtcatgc aatatagggc caagaacaga caaaagatgc
      241 tcgcccgctt cagtgggtac aacgctgcgc gtggttcgcg tcagaagccg caatcccaat
      301 cgttcttcta tacggcgcac tatctggctt aaagctgact gcaccatgct caggcgggct
      361 gctgcgcgag tgaagctacg ctcctctgca acgacgacaa acgccatcag atcagcgatt
      421 tcttcacgct tcattcagta tctccataca atctattaat catttaaatg ataggtctaa
      481 tcatgattta gggtctaatc aacttaatga tcaatatcta tagtgatatc accaaccgtc
      541 aaaacgaacc gacaactcca tgaggaactg aatgatgaat aataaagtca gcttcactaa
      601 cagcaataat ccaaccatct ctttgtctgc agtgatctat ttccctccaa aatttgatga
      661 aacccgtcag tatccggcaa ttgtggtatc tcacccaggt gggggcgtta aagagcaaac
      721 ggccggaacc tatgccgaaa agctggcgga aaagggattt gtcactattg cttatgacgc
      781 atcttatcaa ggtgaaagtg gcggcgagcc gcgtcagtta gaaaacccat acattcgtac
      841 tgaagacgtc agtgccgtga ttgattacct gacaacgctt tcttatgtcg acaatacccg
      901 aattggtgcg atgggaattt gtgccggtgc aggatataca gccaatgctg caattcagga
      961 tcgccgcatc aaggccatcg gtaccgttag tgcggtcaat attggttcga tgttccgcaa
     1021 tggttgggaa aacaatgtga aatctattga tgctctgcca cacattgaag ctggctcaaa
     1081 cgcccgaacc agcgatatta gcagcgaaga atacgccatt atgccactgg ctccaatgaa
     1141 ggaggccgat gcgccgaacg aagaactgcg tcaggcctgg gagtattacc acacacctcg
     1201 tgcgcagtat ccaacagcac cgggttacgc tactttgcgc agccttaacc agattattac
     1261 ctatgatgct taccatatgg cggaagtgta cctgactcag ccgatgcaga ttgtggcggg
     1321 cagccaggca gggagcaaat ggatgagtga cgacctgtac gatcgggcat caagccagga
     1381 taaacgctat cacattgttg aaggcgcaaa ccatatggat ctttatgacg gcaaagccta
     1441 tgtggctgaa gcaatttcag tattagctcc gttctttgag gaaactctgt aatgaaacat
     1501 attcaaatcc gcaatagtga tatggcctgg catattgctg ccaatattca gtttccacca
     1561 aattttgatg agtcaaaaca gtatccggcg attatcagcg tccatccttt tggcagttgt
     1621 aaggagcaga cgtctggcaa tatctatggc aaggcattgg ctgaaaaagg ctatgtcgtt
     1681 ctcgcttatg acgccagttt ccagggagaa tctggtgggt agcctcgttg gatagaagat
     1741 ccgacacagc gcgtagagga tattagccgt gttatcgatt atgcagtaac tctgccatat
     1801 gttgatgctg aacgcattgg cgtgctgggt gtgtgggggg gtggctatgc cattaatgcc
     1861 gcgctgacag aaaaacgaat taaagctgta gtgagtatta cgggtgttaa tatcgggcgt
     1921 ctttttcggg aaggtttcag caattatgat ccgattggcg tattgaatgc aatggcgtca
     1981 caacgagcaa aagaggctcg tggtggagaa ttacagatca acgaattact tcccgccagc
     2041 ctggatgcag caaaagccca tggactgacc gaacgcgatg tttatgaagc aactgactac
     2101 tataaaacac cacgcgggca acaaccggga ggtgcgacaa aaatgttgtt ctcacatgca
     2161 cagaaaacgc tggcctggga tgccttcgca tttactgaag tgttactgac ccagccggtg
     2221 atggtagtag ttggtgaaaa agtcggtgcc ttcggtgctt atcgtgatgg actggaagtc
     2281 tatggccggg cgatggtttc gcaggatcgt cagttagttt cgttacccga tttctcacat
     2341 tatgaactat atgacaaacc ggaagcagtt caggaggcgc tggcaaaagt tattcctttt
     2401 ttcaacaccc atctgggata agtttcatga gaaatattat gaaaaatgta ctgattcttg
     2461 gtgctggcgg tcagatagcc cgccatgtga ttaatcaact ggcagataaa caaactatta
     2521 aacaaacttt atttgcccgt cagccagcaa aaatacacaa accctatcca actaatagcc
     2581 agattattat gggggatgta ctgaatcatg cagcactgaa gcaggccatg cagggacagg
     2641 atattgtgta tgccaacctt acgggggaag atttagatat tcaggcaaat tcagtgatcg
     2701 ctgcgatgaa agcctgtgat gttaaacgtc tgatctttgt tctttccctc ggtatttatg
     2761 atgaagttcc aggaaaattt gtcgaatgga ataatgcagt tattggtgaa cccctaaaac
     2821 cattccgcag ggctgcggat gcaattgagg cttctggact ggaatataca attcttcgcc
     2881 ctgcctggct gactgatgaa gatattattg attatgagct tacctctcgc aatgaaccat
     2941 tcaaaggtac gatcgtatca cgtaagagtg tcgcagcatt aattacagat atcatcgata
     3001 aacctgagaa acacattggt gagaatatag gcatcaatca acccgggact gatggcgata
     3061 agccattctt catgtaaatc aattatatag ataccatcgt actgtactat gtgcagtacg
     3121 atttattaaa cttattaagg tgaactgatc aatcagatgc gattaacatg tgtagaacca
     3181 gtaagaataa aatacgccat gtatataaca ttacgacaat tctaatatct gcacatcaat
     3241 gaaaatgcaa acaacttccg gcaaaaaagt gatacaagat cgatgacata gcgttgaaga
     3301 aaaggatttg ataatccgca aaatttgcac aacactgcct gtaatacttc ctgaaccaga
     3361 gacgatcatg accgtaaccc gctcacgcgc cgaacgcggc gcatttccgc ccggaacaga
     3421 acattacgga cgttcattat tgggcgcgcc gttaatctgg tttcctgccc ctgcagccag
     3481 ccgcgaaagt ggtttgattc tggctggcac tcacggtgat gaaaactctt cggtcgtcac
     3541 gctctcctgt gctctgcgga ctttgacacc ttctttgcgc cgccatcatg tggtgctgtg
     3601 tgtgaatcct gacggctgcc agctggggtt acgggccaat gctaatggtg tggatttaaa
     3661 cagaaacttt ccggcggcga actggaagga aggtgaaacg gtttatcgct ggaacagcgc
     3721 cgctgaagaa cgtgatgtcg tattactgac aggtgacaaa cctggctccg aacctgaaac
     3781 ccaggcgctg tgccagctta tacaccgcat tcaacctgcc tgggtggtct cattccacga
     3841 tccactggct tgtattgaag atcccagaca cagcgaatta ggcgaatggc tggcccagtc
     3901 gtttgaattg cctctggtaa ccagcgtcgg ttatgaaacg cctggctctt tcggcagttg
     3961 gtgtgcagat ctcaacttgc attgcatcac cgcagagttt ccgcccatct cctccgacga
     4021 agccagcgaa aaatatctct ttgcgatggc aaacttgctg cgctggcatc ctaaagatgc
     4081 aattcgcccg tcgtgaactg aagcgccggt tccacatcta ccgccagcca ggtcggtccg
     4141 tcaagatcgg cgaaactgac ctgcggcacc agcggtaaag cggcgctaat ggcacgagag
     4201 gtacacaaca tgcagcccag catcagactg aaaccttgtg cacgcgcttc agtcgccagc
     4261 gcttccgtca gaccaccggt tttatcgagc ttaatgttaa ccatctcata gcgccctttc
     4321 agcgccttca gattgctacg agtatgacaa ctttcatcag cacaaatcgg caacggatga
     4381 ataaaattct ccagcgccgc atcgtcctgc gcaggaagcg gttgttcaag catcgcaacg
     4441 cctaaatccg ccaatagctg gcaacgcgcc gccaaccctt ctgcacgcca ggattcattt
     4501 gcatcaacga tcagcgtggc atcgggcaca gcggtgcgaa ttgccaccat ccgctcactg
     4561 ataagatggt tatccagctt cactttcagt aatttcgcgc ctgcctgcca gagtgttgat
     4621 gcactattgg ccatctgatc aggcgtaccg atgacaaccg tctgtgcagt gataactgtc
     4681 tcgggaagcg ttatgccgat caaatcagcc agcgattgct gctgttttcg cgccgcgaga
     4741 tcccacaatg cacaatccag cgcattacgt gctgcgccgg cagggagaat tttttgcaac
     4801 tcctcccgtg tcagcccttt ctctaattgc ggcacgacgc tcataatttg cgccattacc
     4861 gaggcatcac tttctccata acgcggatac ggcgtgcatt cgccggtgcc tttaataccc
     4921 tcttcttcca gttcaaccac caccacgcgc gcttcactgc gacttccccg ggcaatcaca
     4981 aacggggtat gtaagggcca ggcttcctca aataccttaa cgattctcat ctttactcct
     5041 tgttgtcgcg gtggtctgcg gattaagcgc aaatagcgtt tgctgtgtta ttgacagtta
     5101 gcataaacta ggtgtgacgt taactatatg taaacaggaa agataatcat gtcacaaacc
     5161 gttcatttcc agggcaaccc ggttacagtc gccaattcca tcccgcaggc gggtagcaaa
     5221 gcgcagactt ttactctcgt ggcaaaagat ctgtctgacg tcaccctcgg tcagtttgcg
     5281 ggtaaacgca aagtgctgaa cattttcccg agtattgata ccggtgtttg cgccgcatca
     5341 gtacgtaagt ttaaccaact ggcaactgag atcgacaaca ccgttgtgct gtgtatctct
     5401 gccgatctgc cgttcgccca gtctcgtttc tgcggcgcag aaggtctgaa caacgttatc
     5461 accctctcca ctttccgtaa cgctgaattt ctgcaagcct acggtgtggc aattgctgat
     5521 ggcccactga aaggtctggc agcgcgtgcc gttgtggtta ttgacgaaaa tgacaatgtg
     5581 attttcagcc agctggtgga tgaaatcacc accgagccgg attacgaagc agctctggct
     5641 gtactgaaag cataagttaa tatgcctgat ggtgttgcac catcaggcat attcgcgctt
     5701 actcttcgtt cttcttctga ctcagaccat attcccgcaa cttattggca atcgcggtat
     5761 gtgaaacgcc gagacgtttt gccagtttgc gcgtgctggg ataattgcga taaagctggg
     5821 ttaataccga gcgttcaaaa cggctggtga tttcgtccag cgaaccttcc atcgcatctt
     5881 cgcccacggc taccgttgcg gcgtcataat ccggcaacaa aatatcctgt ggacgcagct
     5941 cataaccgtc cagttgtgtc agtgcgcgat agatagcgtt ctttaactgc cgcacatttc
     6001 ccggccacgc ataacgtgta agtacagtat tcaggtcagc ggccagtttc ggacgcggca
     6061 cgccctgctc gtcggcaaag cgggcgacga acagctcagt taacggcata atgtcctgcg
     6121 gacagtcacg tagcggcggc agattgagcg tcaacacgtt cagacgataa tagagatctt
     6181 cacggaacac gcctttttgc accagttcga ccagattctt ctgcgtagcg caaatcaccc
     6241 gcacatcgac atgcacctca tggtcttcgc caacccgacg gaaagtgcca tcattaagaa
     6301 aacgcagtaa tttcgcctgc atccgtggtg acatttcccc tatttcatcc aacagcaccg
     6361 aaccaccgtt cgcctgctca aagaaccctt tcttcccttc cggagcatga ccaaacagtt
     6421 cactctcgac cgcatcttct ggtatagacg cgcaattcag cgccaggtaa ggtttgcccg
     6481 ctctggggct tgcctgatgg caggcgtagg caaaaagatc tttacctgta cctgtgtcac
     6541 ccgtaatcag cagcggcgcg cttagcatcg ccagtttctg cgcctgttcg acaacgtgct
     6601 tcattttcgg gctgacggcg acaatttggc tgaaggcgct gacgtcctgg gcggcgacat
     6661 tttgcaactg gcggcccata cgaatcgttg atcgcaacat caccaccgca ccggtcagga
     6721 cgtgttgatc attttcatcc tgaagataaa caggcgtaat ctccatcagg aaattctgcc
     6781 cattaataac gacatgctcg ttatgcgaat cttgcggttc gctttccagc caacgtaaaa
     6841 aattaaagcc gttaatcaat tgtgcggcgg tatggttgcg caggcgatcc aatttttgcc
     6901 caaaaagctg acagctcgcc gggttcgcca tatccacttt gcttttcata tcgacagaga
     6961 gcacaggttc aggcaacgcc tccagtaacg cgctcaacgc cagatgctca cgttcggaag
     7021 gcatccacgg gacagtacgc acatcggtaa cacctgcaat acggcgtatt tcggccatca
     7081 gactgctgaa actctcaaac tccagttcag caaaattgag gtagattcgc ccaatgggat
     7141 caatctcaat accgcgtaaa tcaatgcctc ttagcacgag tagatcgagt aattcgcggg
     7201 tcagaccgag tcggtcttca caaaagactt ccagacgcat gggaaccttc acctgaaaaa
     7261 agaacaatta atatgatgat atgacactat ttggatagca ggaaggtttc tgtcaacaat
     7321 cattgacaca aagacggaaa aacgggcact tcggcgtaaa gatatccggt cagcacccga
     7381 tattgcatta tttttcgctg ggcgttttgc ctttttgcag agtttctttc acctgaccga
     7441 taagctgacg acggaaatcc ccaaggcgag gtttgtcatc gtcaatccac ggcagcgggc
     7501 ggcaaagctc catagcttta atcccgagtc gtaccgtcag aagtccagca ccaattccct
     7561 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
     7621 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
     7681 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcaatgcga ttaattaaac
     7741 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
     7801 tcaacgttga ttccgccgcc gaatggctga tttcgcgccg cgcctgggca tctaaaactg
     7861 gctggaccaa atgcgcatac aggctgacca cttcacgatc gttctgcgtt tcatggattg
     7921 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
     7981 tttcgcaaaa tgcgcgacct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
     8041 cgcgttcatg ggcgcgctgt cgcaagcgcc ataagcgccg ccactctgtt accacagaac
     8101 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
     8161 gggtttgcca ggcattcatt gtccactgta taccctgccc gacaacgctt gcgccaaaca
     8221 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcgt
     8281 ccattaccgc ttcgacttgc ccctcttctt cctgcgcttc gtcgagcgtg gccggggcaa
     8341 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgttcga
     8401 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     8461 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     8521 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     8581 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     8641 gttaccgcgc aatgccggaa ttttctcgcc gttgacgtca ataatgccgc tggtggtcgc
     8701 ctgcaccgag gccagcccca ggcaatccat actaatcctt tcaaacgccg cattttgcca
     8761 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     8821 atggtccgct ttcatcgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     8881 aaacagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     8941 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     9001 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accactttct cgcagtaata
     9061 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     9121 ctcgtcccag gcatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     9181 atctcccggc aagacaaagc gcccaggctg aataaagtgc agcccctgct gtttacagtg
     9241 gtggagataa tcggtccaca cggcggcaat gtctgccagc cggttttcgt cggcaggcgc
     9301 tagcgggtca agtccttcgc acatcattcg ccatttcgcc gaccattcgc cgcgctgacc
     9361 attgagtaag cccgtcatct ggcgcgacca gcttaagtaa tcctgcgcca gcatcggcag
     9421 gtcgagcaac cattcgccgg gataatccac aatttccaga tacagcgtgg aggtatcctt
     9481 aaagtggcgc agcagcgaat cgttcgattt aaaacgtaac gccaggcgaa tttcactgac
     9541 gccgcgcgta ggcgtcggcc aggcgggagg atcaccatat aactgcgcca gtccttcatc
     9601 ataggtgaag cgcggaatgc caaagtcacg ctggggaatg cgttttacgc ccagcaagcg
     9661 ctcttcacgc accgcgctta acagcggcaa acgtgctccg gcatgaatat tgagcaactg
     9721 attaaccatc gcggtgataa acgctgtttt gccgctgcgg ctaagtccgg ttacagcgag
     9781 gcgcagatgt ctgtcgacac cccgattcac cagcgcattg agttcatttt taagtcgctt
     9841 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaaat cattcgtaag gatatgaatg
     9901 tgggggccag gccattgatt atcaactgcc cgtgttgtca tcgacgggcg aactccataa
     9961 gatgtgcagc attcacgttc ggacggagtt cctgacctat gtcccctact atttatgata
    10021 ttgccagggt tgcaggcgta tcaaaatcca ccgtatcacg cgtgctgaat aagcaaacta
    10081 atatctcccc ggaagcgcgc gaaaaagtgt tacgggccat tgaagaatta cagtatcagc
    10141 caaacaagct ggcccgcgcg ctgacctctt cgggttttga tgccattatg gtgatttcta
    10201 cccgttcgac caaaactacg gcgggtaatc cgtttttctc tgaagttttg catgccatca
    10261 ccgccaaagc ggaagaagaa ggtttcgacg tgatattaca aacgtcgcac aatccggcag
    10321 aagatctgca aaaatgcgaa agcaaaatta aacagaaaat gattaaaggc attattatgc
    10381 tcagttcgcc agcggataag tcattttttg cccaactcga taaatacgat attccggtgg
    10441 tggtaattgg caaagttgaa ggtcaatatg cccgtgttta ttctgtcgat accgataatt
    10501 ttggtgacag cattgcgttg accgatgcat taattgaaag tgggcatcaa aatattgcct
    10561 gcctgcatgc tccgcttgat gtccatgttt cggttgatcg cgttaatggc tataagcaaa
    10621 gtctgtccgc gcataatatt gcagtgcgtg ataaatggat tgttgacggc ggttataccc
    10681 atgaaacagc actgcaagca gcacggcaat tattaagtca gtcaccgttg cctgaggcag
    10741 tgtttgccac tgacagcctg aaattaatga gcatttatcg tgcggcggca gagaaaaata
    10801 ttgctattcc gcagcagtta gcggtggtgg gttatagtaa tgaaacgctg tcgtttattt
    10861 taacgcctgc accaggcggc atcgatgttc cgacgcagga gttaggtcaa caaagctgcg
    10921 agttattatt ccgcttaatt tcgggaaaac cgtcaccaca gaatattacc gttgccacgc
    10981 atatgacgct gaaataaata ccagctacct tgcctgaatg ccggatgcga tgctggcgca
    11041 tcttatccgg cctacattta tagatcggat aaagtactcg cgccgcatcc ggcaatttag
    11101 cccattatca gaacgagtaa tttacgccga cacctgcata atggaattta tcgctgtcgc
    11161 cttcgtcgtg atcctgccac tcaaacgcgt attccagcga aacggaaagt ccgttctgga
    11221 aatcataacc gtaaaataaa cccacacggt taaaatcatg gccttcacgt tcaatatcat
    11281 cctgccagtc ccagttactc tagcgatcca gcccaatgcg cgtatacggc gtcaccgagt
    11341 ggttgccgag cgtcagcggc aaataggcgc gaatttcttg tgtggaaaac tcaccgttat
    11401 tgcggctgtc gtccatattg aagccgcgct cgagataata gttcactcgc aaagcaaccg
    11461 tttcgttgaa ggtatattgc agacctgttt ccgtttcgac acgggtatca gcgtaaccgg
    11521 tcgtgttcaa atcgttggca aatttataca tcgacaacca accgttgaaa cgtaaatcat
    11581 cagtcagttt cacatcccag tctggcgcga ttttccagcg ctgcatattc gccgtgtctt
    11641 tgcccggttc atcaacgtag tgataaccat aattacggaa accgccggtc aggccgaaac
    11701 tgaaatcatc gctttcgagg aactgataat gcacctccag ctccgggcga tcaaacctcg
    11761 ttccacgttt acccgcgcta taatctaccg gcccttcctg ataataggcc agagcaattc
    11821 tccacggccc gttggcggca ttaaaataga ctgaaggctc cgccagccca tccatatctt
    11881 cgccataacc ctcgacgttt tctatttcgt acatcgcacc gatattaaag tgccagtcgc
    11941 tcttttcctc ggcctgtgcg caggccattc ccgcacacat caccagtgcg gtacagggta
    12001 ataacttttt ttgttccttg tattatcctg ggggaatgcc ttccccctgt atttagcgaa
    12061 ttgctatttc cgtttctgca tcaaagaaat gacatttcgt catatcaaaa tgaatagtga
    12121 tattttctcc tgcatgataa tcatttaacg caccagcacg gaccactaac tcgtgccccc
    12181 aaccgtggtg tagagcataa attccgcacc ggttaattct gccacgctaa ttttggcgga
    12241 aatgttattt tcctcttgcg cgtccggatg aatatcttcc gggcgtattc ccatcacgat
    12301 gggcttatgc aaactttcct gtgttttcag aaccgctaat ttctcttcga gaatggttaa
    12361 tttaagcgtt tccgtaacga atttatcgcc atcgatcgtg ccgcgaataa aattcatcgc
    12421 tggtgatcca ataaatccgg caacaaacat attcgcgggt tggttataaa cggttttcgg
    12481 cgcacctact tgctgaacaa tcccgtcttt cataatcaca atccgcgccg ccatggtcat
    12541 cgcttcggtc tgatcgtggg tcacgtagat catggtggtg ttcagtttct gatgcagctt
    12601 gctgatctct gcacgcattt gtacgcgcag cttggcatca aggttagaga gcggttcatc
    12661 cattaaaaac acgcccgctt cgcgcacaat cgcccgcccg agcgccacac gctggcgttg
    12721 cccgccggaa agcgcgcccg gcttgcgttt caggtactca cgcaggccga gaatttgcgc
    12781 cgcccagttc acccgctcat caatcacctc tttggcgatt ttttgcatct tcaggccaaa
    12841 cgccatgttg tcgtaaaccg tcatatgcgg atacaacgcg tagttctgga acaccatcgc
    12901 gatattgcgt gctttggctg gaacgtcatt cattcgtttg ccgtcgatca acagatcgcc
    12961 gccgctgatc tcctcaagcc cggcaatcat gcgcagggtg gtcgatttac cgcagcccga
    13021 cgggccgaca aatacgatga actctttatc ggcaatttcc aggttgaagt ccttcaccac
    13081 atgcacctgg ttatcgtaga ttttttgaat atgttgtaac gaaagctgag ccatgttgat
    13141 tcctttgcta tacgttttgc cagaaggccg ataaccgcga ccaggtcagg gagtccgttg
    13201 agggcaacag taattgcgcc ccggttaagc ccgcaccgat ccccaccgag cgcataccgc
    13261 tggcgttaat ggcgtcaatg cccgcctgcg catcttcaat gccgatacat gcctgcggcg
    13321 gcacgcccag cccggcacag gcagcgagaa agatttccgg atccggtttc gagtttttaa
    13381 gttgggaagc atccgcgcag aaggtgaaaa actcgcgcag ctccagcgcc gctaaaatcg
    13441 tcggcgcatt cagggagacg gaagccagcc ctaccggaat ctgctgtgca cggagctctg
    13501 ccagcaaatt gcgaataccg ggcagcacag cattaaccgt cagctcgcgc agtgagtgga
    13561 cgtaaagcag atttttgcga tacgccagtt gcgcccgctc ctgcgggtta aagtcgccct
    13621 ctttgccccc gtgttgcaga atgcgccgca gagactcatc gcggctgatc ccttttagag
    13681 attcgttaaa ctgcgcatca atactgatgc caatttcagc ggcaatctgc tgccacgcct
    13741 ggaaatgcag atgcgcggta tcggtgatta caccatccag atcgaaaatt accccttgca
    13801 gtttcattca tcctcctgat gcgtggtagc ggtcccattg aagggcaaaa taaaatcacc
    13861 caaacagaaa acagattctt cagccacgga tataagctga ccgttcaaac gcagtgaaac
    13921 gggcgcagaa gttcgaatcg caatacgctg cgcgtcgaga gtgacctgta attcgcagcc
    13981 ctgccagaac agagggaaag acaactgttg ccactgctca agtaacgccg gatttagatg
    14041 caattcaccg tcacgcacgc tcaccccggc aaaaccctga atcgccccca gccagatagc
    14101 gccagttgcg gcagcatgga taccatcatc acaactatgc ggatcagcac caagatcgat
    14161 ttcagtcccc tcgcgccaga actgataact ttgggtcagc aggccacagc gtgcagcaac
    14221 aatgccgtgg attgctttac ttaatgacga gtcgtgaata gtgcgcggtt cataaaattg
    14281 cagattggca agacacgatg ccgatgagaa ctgctctggc agcatgtaat tgagcatcac
    14341 cacatcagct tgtttgagga tctgcatctc gttcacttct gcgcgtgaat aatccagcag
    14401 aatggtttgc ttccccgccg ccgctttgta tttcgccaga ttaatcgccg gcttagtcat
    14461 aaacgaatca tcctgcggca aaacaccgtc gggctgaatt tctggcagcc gcagttcttt
    14521 aaggaacatt tcggcacgat gaataaacgc gtcgtcacta cagccaaact ggcgggcaat
    14581 actcagcgcc tgctggacgt tgtaatacac catatagctg gtgaaggcgt tattattgac
    14641 atgttcggta tattcgtctg gcccaataac atcatgaatt tccagacggt cgttaaccct
    14701 caccgcgcgg ctaatccaga actttgcagt ttccagaagt agcgccatgc cttcatgcgc
    14761 aatgaaactt tcatcccccg tggtctgcca gtattgaata accgcccagg cgatatcggc
    14821 caccagatga tgttccgcct gcgccgaggc cactttttgc cgcagcccgg tgcgaatgtt
    14881 aatggcggca aattccggcg tctcttcttc gccgctgcgc gcgctttccc acggaaatag
    14941 cgcgccctgc cagccgttgc gtcgcgcttt ctcctgcgcg cctggcaagt tgtgccagcg
    15001 ataacgcagt aaacttcggg caaccgtcgg atcgctaaac agatggaacg gcagcaaaaa
    15061 tacttctgta tcccagaaaa cgtggccttt gtagccttcg ccggttaagc cttttgccgc
    15121 aatactgctg cgctcgccgt gagccggcgt catgatgcgc agatgataaa gcgcataatc
    15181 taacgcttgc tgatcgtgcg catcgccgcc atttaccgtg atacgacgtt tctgccacca
    15241 ttgacgccag ttttctgttg atgctgcaag atgttggtcg taactctgtt gcgcgcacat
    15301 ttcaagctgg cgaagcgaca cgctgcccca ctcgtctaaa gcagcttgcc tgtcatcccg
    15361 ccagtcgatc cacaccagtt tttgcaacgt cactgtctcg cctgcatgaa gctgcgtact
    15421 ggtatgttgc agtaaacggc gctctttggc ggtatagcat tgctgcacat caccgctcac
    15481 ctggcagcaa caactgatgg ccacatcact gcgcccatcc tgggtggtgt agatcccctg
    15541 catcaaatgc tgaccaaaca cccgcacctg ggtttcgtcg agatgctggc gaccgtggtt
    15601 ggtttgcgtg gcgtcaatgc ctgttgaaat cagcactgac gcgtcggcgt ccagtggcat
    15661 aatagtgatt tccagcgcaa tgagcggcag ttggtctgcc gaaacaaagc gacggctggc
    15721 gatggtgtaa cctgcgccgt tgctggtacg ccagacaacg ctacggcgta attctccact
    15781 ggcaaagtca agttcacgct gccaggcttc gcgggataac gagaaaacct caccatttat
    15841 ggcaatctcc atccccaaga tatcaggcag attcaccagt tcgttgattt caccttttcc
    15901 cgcccgatga tacagccccg ccagatacat ccctcgcgtc tggcgcgtgt aatcttcttc
    15961 atggctggcg cgaagcccaa gatagccgtt cccctgcgcc atcagcgatg catacttgtt
    16021 cagggtatgc tggctgaaat ggggttctga taacgttact ggcctggtca taattccaca
    16081 cgcgtccctg tttctgcgga ttgatataac gccgccacca gttgctggat gatgtacccc
    16141 tgctcggcgt cggctatcat cacgggcttg ccctgtacgt gattgataaa ggcttccatg
    16201 ctgcgcaaat ggcggttgtc gtctgccatt tcccgttgca tcagcgtcat taattcaccg
    16261 ttgttatcgg tgtagatatg tgctggaaac agcgtcgcac cggctttatc accacaaaag
    16321 ctgacgttca taatcaactg ttcgcggatg ttgagtgcaa atgacgtttc cagccacaga
    16381 atgccgccgt tatgaaattc aatggtgcca aacagcgaat cttcgacgct gtaagttgcc
    16441 ggatcccact cgccaaattg accacagctc ttttgcgtgc cgatcttttg aaaactatgc
    16501 gcattcacgc ttttcaccgc cggaaagccc agcacataca tcgcagcatc cagcatatga
    16561 atgccgatgt cgatcagcag gccaccaccc tgcagttctt tattggtaaa gacaccccag
    16621 ccgggaacgc cgcagcgacg cagggcgcgg gcggtggtaa cgtaaatctc tcccaaaacg
    16681 ccgttggtca cctgttcacg cagctgttgc gtatcgagcg caaacgatgg tgaaagtcgt
    16741 aggccagcac cttgcccagt ttgcgcgcgg tatcgcacat ttcccacgcc tgttctgtcg
    16801 tcatggcggg cggtttttcg cacatcacat ggcagcctgc ttccagtgcc atcagggtat
    16861 gttcgtaatg aaaacggtta ggtgagccga cgctaaccac atcaggtttc accgccagca
    16921 gcatggcctg cggatcgtcc cacacggatg cattcccgta tttttctgcc agcgcctgcg
    16981 cctgggaaag gcggctgtca cagacagcca ccagttccag atcgttgcgg gtgcagtagt
    17041 acgaagcatg aaccttatcc gccacctggc ccgcgcctat tatcgcgacc cgcagcggag
    17101 agcttgtcat tgcacttttc acaaagacct cttagcaggt acgcaaccag gccaacgaat
    17161 cacggtacgc ctgggcagga tcttctgccc ggatacgccc ttcatacacc acataaccct
    17221 gatagttatc ggcgcgcagc tgttcaaaca gcgcgtggaa atcgagggtg ccactgcccg
    17281 gctggtaacg atggttatcg gcaatatgca catgaccgag caagtcacgg ttgtcgtgca
    17341 gcgcctgcgc caggttatct tcttcgatgt tcatgtgata gaaatcgccg ataatctgca
    17401 catgtttaag attgttttcg acgatgtaac gacgagcatc ggcgagggtg ttgatcatat
    17461 gatcctgata gcggtttaac ggttcgagat acaccacggt tccggtacgc gcggcgacct
    17521 gttccagtac gcgcagggaa tcactcacca gtttgcggtc gccgtccagg ctacgcggcg
    17581 aggtcatcgg cggtaagcgg aaggtaaaca tgccccacgc tgccggaaac cccccacttc
    17641 tgccagcgcc tcgagaatgc gttcgatctg ctttaagcca ttaagacgac gctcttcgat
    17701 aaagtcacca atccagccgt catagccgcc gcaggcggtg gtcactggta aaccggtttc
    17761 tttgatcgcc gctttgactt cttcgatgtt gttgaccagc agtttgccgt caatctcaaa
    17821 accatcgaag cccatctctt tgatataacg aaatctctcg cggatatttt ccggaaagaa
    17881 tgcctgattc tgtgtgccga ttttcatttt ttcattcctt tgaattaaaa cgtaacgccc
    17941 attttgatgc tctgttccgg atgctgatcg acatactgca tgtagctttc cgggctgttg
    18001 gcaaaggtca ctaccgggtc gattaaatct tcgcaattga gataaccgtt catcagcagt
    18061 tcccagcagg tttcttcaat acgcttacgg ctccagcgcg gataatccgg gttcggttcg
    18121 ctgcacgcgc gggagaaaac aattttggcg ttattgaaat gcgcttcgcg tccgaggtta
    18181 aaaccttcag caaatggttt ggcaaacgcg acataggaga tggtgccgcc gtaggccagg
    18241 ccgcgcagcg ccgattgcag cgcgtcggcg taaccgctgg tttcgataat cacatcagcc
    18301 ccctgcttgc cggtcagcgt tttgatctct ttgcctacgt cagtgccaat ggggttaagg
    18361 cagaaatcag caccgtggcg acgggcaata tcacagcgat gggcgatagg atcgacgcca
    18421 attaccaccg atgcgccagc gcgtttcgcc agttggatgg caatttgacc gatcgcgcca
    18481 agccctacca ccacgacaaa atcccctacg cgtacgttgg catcgcgcac gccgctcatg
    18541 gcaaactgcg ccgggtcgta acagacggcg tttttccagg agctgccttg cggcattttg
    18601 cgcagcttgt agttattcac tgcgttaatg atgaccgtct cggagagcgg gccgtagccg
    18661 catacgctgt cgcccaccgc gtagtctgta acgtcgctgc cgcactcgat aatgtcgcca
    18721 accaccatgt tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc atctgccgga
    18781 cgcggggtga acatctgcca ctcgccgtta aagtcttcat cgataaacgg gctggcggcg
    18841 cggaagtcga ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg gatcttcact
    18901 tcattggcta aaatcgcccg atcttcatac tcaaccagcg cagcaacacg cggtgctgtg
    18961 gctactaact ttttcatgta aaactccttg taaagaaact ggccgcttta gcccttcacg
    19021 ccaccggcgg tcaaaccact cttaataaaa cgttccgaaa gcgcatacat aatcaccacc
    19081 ggcaatgcgg tcaccagcga ggccgccatc atccgtcccc agatgtaatc aggcgtactg
    19141 aacagcgcgt tcaggcccac tggtaaggtg aaattgctgg cgctggagag gaaaatcgac
    19201 gcaaacagat agtcgttcca cgccaccata aagcagtaga cgaacaccga tatcagcccg
    19261 gacatcgcca ggggcacggt gatacggaag atgatttgca gacggttgag gccatccatc
    19321 atcgccgctt cttcgatctc atcagggatg gtgtcgaagt aacttttcag cataaacacg
    19381 gcggtgggca gggtttgcgt caccatggta atgatcagcg ccatctcggt gtcgtaaata
    19441 ccaagcgcgg tgatgatttt gaacagaggc accaccagca aaatgccaga gaacatatac
    19501 accgtgtaaa agctggcgtt gatggtcatc cggcctttaa agcgcaggcg ggaaagcgcg
    19561 taagctccga gaatgccgag aaataccgcc accacggagg aaaccaccga caccaccatg
    19621 ctgttacgga agtagtcgac aaacgggaaa atcatcgggt taaaaatgtc gacgtaatgc
    19681 tccagcgtcc actgttgcgg cagcagcgta ggatgcagcg atatcgcctc tttcgcgctt
    19741 ttgaacgagg tcatcagcat cacaaaaaac ggaaacagcg tgatgatgag aaacagcgcc
    19801 agcccgcagt aaaaaccgat acgactgagc gtgcgtttat ttgttgccat tgaggttcac
    19861 ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg aagagcacca ccgagatcgc
    19921 cgccgctttc cctaagtcat taaaggcaaa ggcggttttg tagagataga caccaagaat
    19981 atcgaccttg gttgtcagca gataaacatc agcgaacatg tagaacatcc agatggtgcg
    20041 cagggtcacc accgtcgcca ggaccggcat aattgcgggc agcgtgacga tgcgaaaccg
    20101 ctgccaggca ttagcgccat ccatctctgc cgcttcgtac agcgatttgt cgatggtctg
    20161 caaaatcgcc agaaacgaga taaaggcata cgggaagtag cgccagatgg cgaacagcac
    20221 caccagcaca aagctactgc ccggattatc aaaccacagc ggtgcctgct catagagatg
    20281 cagaagatcg acgccgaggt agttcacaat gccgtagccg ttgttgaaca tgtatttcca
    20341 ggcaaacacc agcgaaatgg acggcgttac gtaggagagg atcaccagcg aacgcgcggt
    20401 tttgcgcagg cggaactcgc ggttgaaaaa catcgccacg gccagcccga gcacggtgct
    20461 gcccgccacc accagcgccg tataccagac cgtcatccac agcgagtgcc agaagccggg
    20521 atcggagaga atgcgcacat agttactcac cccaacaaac gttgactcga tgttgggatt
    20581 gagcggcaga cgtaaaaaac tgatttcgat attcgacacc atcggccacg ccaccagacc
    20641 gcccagcagt aataagctgg gcgcgagaag cagcagcgca aacggcatat cggaacgacc
    20701 tgaaaaaagc ctgttcattg cggattcctg tcctgggttt agtgctgttc gatcagttca
    20761 tccagctttt tctggctcgc ttgcagcgtg ccagggagat cggctttacc cacggtgacg
    20821 ttatgcacca tggagctcac cacaccagaa cccgtcacat cgcccatgcg ggtaaagttt
    20881 ttatccccta ctgcgccaaa gacctgaata tttggcagtt caccgattag ctgattcggt
    20941 agttcaccca gcgccttaat aacgtcgttg tctttccagg tggcggtagt gacaaccgct
    21001 ttattcaccg gcagcgcagc acctggcgac atcatcaccc agtcggcaat gttgtccgcc
    21061 tgctccataa aggtgacaaa tttttctgct gcttcggtct tttgtccggc ggtaatggtc
    21121 agcgaggtca acatgccgta gaccgcagag tttttctcgg ttggcaccac gaaaccgacg
    21181 tttttcgggt cgccttcttt aatcacagcc ggaaggatat aggtggagta aatcgccatc
    21241 ggcgcggtgc cgttcataaa ggcgtctttc acttccatga tgtcgttaga acccggcatg
    21301 gtgttggcag caaggtcgcg gtaataggtc agcgcctgca tcatctctgg tgtatcaagg
    21361 gtgattttgc cttcggcgtt aaagacgttg gcctagttgg ataacgcaaa ctgggagaag
    21421 gattgttccg tcagcacgct ttctgccgta ggcagcgcaa taccgtattt tttattcgcc
    21481 gggtcattca gtttctgtgc aacgtccagc agctgttgcc agtttttcgg ctcctccagc
    21541 cccgcttttg ccagcacatc tttgcgatac cagatgtcgc caatccaggc gctgacagga
    21601 acaccggtcc atgcgctacc atcttcggta cgcacaatac gcagtacgcc atcgtaaaac
    21661 gcgccttcac caacgttgct gatgaccgtg gcaaccgctt tgcgatcgat aagctgctct
    21721 ttatccataa ctttggcgta gtcatggctg gtttcgatca cttccggcag cgagccgcta
    21781 cgtgaaaggg tgatgacttt ggtgttatga gcgtcttctt ccacgggcac ttgtttaacg
    21841 gtgatacctg ggttttcttt ttcaaaacgg gcaatcaatt tgctgataac tgcctgacgc
    21901 tcctgctcca ccgaagaatg cataaattcg atggatacat gggttttatt ttcttcttta
    21961 cagcctgaaa tcagggcgca ggaaaccagt gctgataaca gcacgatttt tgatttaatc
    22021 atgagatgtt cctttttatt taatccacat aacctgccag gggcgcagcg ttatttcatt
    22081 acccaatata tcaacttcac taatcagatc ccgaccatgc agatcagtaa tatttacatg
    22141 ctgaatattt ttactgacat taaacaatcc agtcagacaa ttaccatcag cgttacttct
    22201 ttgaatacgc attacggatg aattaaccgt atcgatggta aaattattat ccggatgaaa
    22261 ctcgttatgg ctgcgacgaa gtgtaattaa acgcgacaac tcatgatata ccgcatgcct
    22321 taatgtagct tcatcgttca gttctcgtgt tatctcttca ctgtgatatt ttttacggtt
    22381 aatcgcacgg ttatatccga gtttttcgac acctgcgtaa tcattacgcg agccaagaat
    22441 actttgaata tatatcgcgg gtacaccggg gaaacttaac aaaatcgcat gggcaaggat
    22501 aaacctggtg caacgttctt catcgctact ctcgcggcgg cttaacgcat tcatataggt
    22561 gacattcatc tcatagggac tgcgcgtgcc gtcgggatta tttttccagt ttacta
//
LOCUS       DAJVRP010000064        22218 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVRP010000064 DAJVRP010000000
VERSION     DAJVRP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.064"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..510)
                     /locus_tag="OQN12_002549"
     CDS             complement(<1..510)
                     /locus_tag="OQN12_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1654825.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVS"
     gene            complement(534..731)
                     /locus_tag="OQN12_002550"
     CDS             complement(534..731)
                     /locus_tag="OQN12_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS1654826.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            complement(903..1823)
                     /gene="lpxL"
                     /locus_tag="OQN12_002551"
     CDS             complement(903..1823)
                     /gene="lpxL"
                     /locus_tag="OQN12_002551"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1654827.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            2048..3100
                     /locus_tag="OQN12_002552"
     CDS             2048..3100
                     /locus_tag="OQN12_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS1654828.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            complement(3142..3717)
                     /locus_tag="OQN12_002553"
     CDS             complement(3142..3717)
                     /locus_tag="OQN12_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1654829.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3721..4287)
                     /locus_tag="OQN12_002554"
     CDS             complement(3721..4287)
                     /locus_tag="OQN12_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS1654830.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            complement(4548..4661)
                     /locus_tag="OQN12_002555"
     CDS             complement(4548..4661)
                     /locus_tag="OQN12_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCS1654831.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            complement(4709..5827)
                     /gene="solA"
                     /locus_tag="OQN12_002556"
     CDS             complement(4709..5827)
                     /gene="solA"
                     /locus_tag="OQN12_002556"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1654832.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            complement(5942..6196)
                     /gene="bssS"
                     /locus_tag="OQN12_002557"
     CDS             complement(5942..6196)
                     /gene="bssS"
                     /locus_tag="OQN12_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1654833.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            complement(6486..6731)
                     /gene="dinI"
                     /locus_tag="OQN12_002558"
     CDS             complement(6486..6731)
                     /gene="dinI"
                     /locus_tag="OQN12_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1654834.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            complement(6805..7851)
                     /gene="pyrC"
                     /locus_tag="OQN12_002559"
     CDS             complement(6805..7851)
                     /gene="pyrC"
                     /locus_tag="OQN12_002559"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1654835.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            complement(7957..8517)
                     /locus_tag="OQN12_002560"
     CDS             complement(7957..8517)
                     /locus_tag="OQN12_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1654836.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            complement(8651..9298)
                     /gene="grxB"
                     /locus_tag="OQN12_002561"
     CDS             complement(8651..9298)
                     /gene="grxB"
                     /locus_tag="OQN12_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1654837.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            complement(9362..10570)
                     /gene="mdtH"
                     /locus_tag="OQN12_002562"
     CDS             complement(9362..10570)
                     /gene="mdtH"
                     /locus_tag="OQN12_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1654838.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            10806..11390
                     /gene="rimJ"
                     /locus_tag="OQN12_002563"
     CDS             10806..11390
                     /gene="rimJ"
                     /locus_tag="OQN12_002563"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1654839.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            11401..12048
                     /locus_tag="OQN12_002564"
                     /pseudo
     CDS             11401..12048
                     /locus_tag="OQN12_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            12050..12916
                     /locus_tag="OQN12_002565"
     CDS             12050..12916
                     /locus_tag="OQN12_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1654840.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            13083..14618
                     /gene="murJ"
                     /locus_tag="OQN12_002566"
     CDS             13083..14618
                     /gene="murJ"
                     /locus_tag="OQN12_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1654841.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(14658..15074)
                     /gene="flgN"
                     /locus_tag="OQN12_002567"
     CDS             complement(14658..15074)
                     /gene="flgN"
                     /locus_tag="OQN12_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1654842.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(15079..15372)
                     /gene="flgM"
                     /locus_tag="OQN12_002568"
     CDS             complement(15079..15372)
                     /gene="flgM"
                     /locus_tag="OQN12_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS1654843.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            complement(15448..16107)
                     /gene="flgA"
                     /locus_tag="OQN12_002569"
     CDS             complement(15448..16107)
                     /gene="flgA"
                     /locus_tag="OQN12_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1654844.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            16262..16678
                     /gene="flgB"
                     /locus_tag="OQN12_002570"
     CDS             16262..16678
                     /gene="flgB"
                     /locus_tag="OQN12_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS1654845.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            16682..17085
                     /gene="flgC"
                     /locus_tag="OQN12_002571"
                     /pseudo
     CDS             16682..17085
                     /gene="flgC"
                     /locus_tag="OQN12_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            17097..17792
                     /gene="flgD"
                     /locus_tag="OQN12_002572"
     CDS             17097..17792
                     /gene="flgD"
                     /locus_tag="OQN12_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS1654846.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            17817..19025
                     /gene="flgE"
                     /locus_tag="OQN12_002573"
     CDS             17817..19025
                     /gene="flgE"
                     /locus_tag="OQN12_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1654847.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            19045..19796
                     /gene="flgF"
                     /locus_tag="OQN12_002574"
                     /pseudo
     CDS             19045..19796
                     /gene="flgF"
                     /locus_tag="OQN12_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            19934..20716
                     /gene="flgG"
                     /locus_tag="OQN12_002575"
     CDS             19934..20716
                     /gene="flgG"
                     /locus_tag="OQN12_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCS1654848.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            20769..21467
                     /gene="flgH"
                     /locus_tag="OQN12_002576"
     CDS             20769..21467
                     /gene="flgH"
                     /locus_tag="OQN12_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1654849.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            21479..>22218
                     /gene="flgI"
                     /locus_tag="OQN12_002577"
     CDS             21479..>22218
                     /gene="flgI"
                     /locus_tag="OQN12_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1654850.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVT"
BASE COUNT         5475 a         5551 c         5778 g         5414 t
ORIGIN      
        1 agataccttg ataccttcgg agatgagttc ttttttacgg ttccatgcag cttggacacg
       61 ctcagacttt ctcaaacttt cctcatgcgc ccgctgcatt atcagaatgc ttttaatcag
      121 ggctaacggg tcttttaaac tttctcgtgt gtaatgttct ccatcgctca gtgtgactac
      181 atctacacca gcccttaaga tacggcgtaa tagctcagaa gcaatatcaa tatcctgacg
      241 tgacagacgg tctaaactct cgattagtag cgtatcacca gctttaacaa gccctttctc
      301 gatagcggtt aaaaagtcac ctagaccacc tttcaggtgc ttaccactaa aagcactttt
      361 ccccaaatct ttaaagctca aggatgaatc caagtaaaac tcagggttct tatcaagcca
      421 gtcagtaacc agcttagatt gtcggcgtac agagtcccct ttcagttgtc gttcacttga
      481 aaatctgatg tagctgattg cccgtctcat attcaatacc taaaatgatg tgtttaggaa
      541 cccattatac tgaagacaat accggaccac atattcaggg cggagtggcc ggtaacacca
      601 agctgctcaa cgtagagggg taagaagggc attaccagac tgaaggcggc cccggtaaga
      661 aaacagccta gccaggcgac gatcaggttt cgtttccagt ttataggggt gtcattttca
      721 cagggtgaca tagcaatccg ctgttggtgc gccaggcgcg gtgaacataa gaagaaaaga
      781 taaacacact aattatgcgc ccgacttcca gggggcgcaa tccagagagc ttttatcgct
      841 aaatcagggg gatttgctgt ggtaatgccg gatgccattc tgaagcatcc ggcatgggag
      901 atttaatagc gtgaaggaac gccttccggg cgtgttttaa agcgacggtg taaccacata
      961 tactgctctg gtgccatcat gatgcatttt tcgaccactt tgttcatcca cgcggcggta
     1021 gtttcggcat catccagtgg cggagaacac tctggtggca gcataatcaa ttgataccct
     1081 ttgccatctg gcttacggcg tggaacgaag ggcaccagac atgcgccgga catccgtgcc
     1141 agcatccagg ttccggtcgt ggtcgcagcc tgctcaacgg caaataacgg gacgaaaacg
     1201 cttgagcgcg ggccgtaatc atgatctggt gcgtaccaga ccacttcgcc ttttttcagg
     1261 gctttaatca tgccttttaa atctttgcgg tcgagcatcg atttatttga gcgcaaacgg
     1321 ccccaggttt gtagccagtc aatcagtgga ttatcgttcg ggcggtaaac gccaatcccc
     1381 ggttcctgca taccaaactg ccgcgcgccc agctccagtg tcagaaaatg gatgccaact
     1441 aacaggatgc cgcgtttttg cgcctgcacg tcacgaatgt gttccatgcc gatcacttcc
     1501 gtccagcggg cgattcggcg gtccggccag aaccacgcca taccggtttc catcaggccc
     1561 atgccaacgg attcgaaatt cttcaccacc attttacggc gttcttgttc gctcatttcc
     1621 gggaagcaca gttccaggtt gcgatgcaca atttttgcgc gtcgtttcat aaaacgtaac
     1681 gccagttttc ctaatccaca accgaggcgg tagataatcg ggtagggcaa ttgcacgacc
     1741 aaccaaagta cgccaatacc caaccaggtt aaccaataac gcggatgaag cagtgcggtg
     1801 gagaacttgg gtagattcgt catatcaatc ctgtttttca acctatacgg gcaattgtat
     1861 gtattgtcgc attttttcgc ccgcaaccaa aatttgtggc tgaagactgg gcgaaattgc
     1921 cgcgcttgta aataacaaat aatttttaat gcgcaaatgt agcgtaaaat gtgtggatgt
     1981 taattatcga taattgctat atcatgccgc ggatttttac tttcccatct cgcaggaacc
     2041 gtacaccatg ccagtgttac acaaccgcat ttccaacgac gcgttaaaag ccaaaatgtt
     2101 ggctgagagc gaaccgcgaa ccaccatttc gttttacaag tatttccaca tcgccgatcc
     2161 taaggcgacc cgtgacgctt tatatcagct gtttaccgcg ctgaatgttt ttgggcgagt
     2221 gtatctggcg catgagggca ttaacgcgca aatcagcgta cctgcgagca atgttgaaac
     2281 atttcgcgcg cagctctatg ccttcgaccc ggctttagag ggtttacgcc tgaatatcgc
     2341 gttggatgat gacgggaaat ccttctgggt actgcgcatg aaggtacgcg atcgcattgt
     2401 tgccgacggt attgacgatc ctcactttga tgccagcaat gttggtgagt atctgcaagc
     2461 ggcggaagtg aacgccatgc ttgacgatcc cgatgcacta tttatcgaca tgcgtaacca
     2521 ctatgagtat gaagtggggc actttgaaaa cgcgctcgaa attccggcag ataccttccg
     2581 tgagcagctg ccaaaagcag tcgagatgat gcaggcacat aaagataaaa aaatcgtcat
     2641 gtactgcacc ggcggcattc gttgtgaaaa ggccagtgcc tggatgaaac ataacggatt
     2701 caacaaagtc tggcatatcg agggcggaat tattgaatac gcccgtaagg cgcgcgagca
     2761 gggcttgccg gtgcgtttta ttggcaaaaa ttttgttttt gacgagcgga tgggcgaacg
     2821 tatatcggat gagattatcg cgcattgcca ccagtgcggc gcgccgtgcg acagccatac
     2881 caactgtaaa aatgatggct gccacctgct gtttattcag tgtccagtat gcgcggaaaa
     2941 atacaaaggt tgttgtagtg agatttgctg cgaagaaagc gcgttaccgc cagaagaaca
     3001 gcgacgccgt cgggcaggac gtgaaaatgg caataagatc tttaataagt ctcgtggacg
     3061 tctgaataca acactgggta ttcctgatcc aacagaataa atatcattgc cggatgcgtg
     3121 ccatccggca acatttcact cttacttctg ctgtacgcct tccactgaaa taatcagatc
     3181 cacttcctga gaagctggac ctaaatctgt cttgatattg aagtctttga gcttaatttt
     3241 gccttcggcc tcaaagcctg cacgtttacc gccccatggg tcgtcgccct gaccaattaa
     3301 tttcgcttcc agcgtgacgg gtttggttac gccattcagc gtcagatcgc cggtaatatc
     3361 cagttcgtca ccgtctttct tcacgctggt ggaggtgaat gttgcctgtg gatattttgt
     3421 ggtattgaga aaatccgcac tgcgaagatg tttatcgcgt tcggcgtggt tagtatcgac
     3481 gctggtggtg ttaattgtca cattcacttt atcggcagcc ggattttttt catcaaaggt
     3541 aaaagtaccg tcgaaatctt taaaggtgcc atataaccag ctatagccaa gatgctgtat
     3601 gcggaagtta acaaaggcgt gctgaccttc tttgtcaatt ttgtaatcgg cggcaaccgc
     3661 tgaaccggca gagaacatca gggacgcgaa ggttaaacca agcaggcttt ttttcatttt
     3721 ttatactcca tagtcagatg acgactttcc cagcatgcgc ttcagagtgt cgtctttatc
     3781 gatgaaatga tgcttcaggg ccataaatcc gtgcataaca gacagtacta cgacgctcca
     3841 cgcgagccaa aaatgcaggg caccggcaaa gtctgcctgt gcgccagcgt cggcaagggt
     3901 cgcggggacg tcaaaccagc caaaaacgct gatcggttta ccatcggcag ttgagatcag
     3961 atagccgctg atgccgatgg cgaacagcaa taggtacaat gcgagatgtc cagcttttgc
     4021 ggcaagacga gtcatgggcg aataactcgg cagcggaccc ggcggtggag atatgacacg
     4081 ccacagaacg cgaataacca gccccatcat taacaaaata ccgatgcttt tatgcagttc
     4141 gggtgctttg tgataccagc catcgtaata actgagcgtg accatccaca gacctaaagc
     4201 aaacatgccg tagacaataa ttgcgcttag ccaatggaag gccgcagata taacaccata
     4261 gcgttcaggg gtatttgtga atgacataag tgcatccaat acgaatttgc gagagaaaga
     4321 aaatggcgtg tcggagaatg aattgcaaca caaaaataac aaattgaatg atatttattt
     4381 ttatttcaat aattttgaat taataatgaa agaatcttca taagtttagt gggttcagca
     4441 tcagagatgt ctggtgagtg ctgtagaagc aggaataata tcattcaatt tttcacgcta
     4501 aatgaaaggg agattaaata tttgtcagtt tatgtcagtg cggaaaacta aaaataaaac
     4561 acatagatca gatccataat tgccaacaat ccccagagaa aaagataaag catcaaatgc
     4621 tcgcgaatat tattgatgag atagtgcaac agccgacgca ttaaatactc ctgtaaaaac
     4681 ggcctcattc tggaggccgt aatgatgatt attggaagcg ggaaagcctg aatggcgtca
     4741 aatcaaaatc gcttttttgg tcttgcgcaa aatcggcagc tatttcccct aaaactgacg
     4801 caaatttaaa accgtgccca ctcaggccgg taatgagcag tgtattatcg tggccgggta
     4861 gggtatcgat aataaaatct tcgtcaggcg aattatcata ggtgcaggca gcgccgtaca
     4921 ggcagcaacc gatacccggc aatacattgc gcaagaacgg gaaggcttct gacccatcac
     4981 tggccacttc cgcaaacgga acacgttcat ccgctgaatg gataacctgg cctccgttat
     5041 gtttgccaat cttcaacgcg tcgttttctg ccggaaaacc ataatattga tcgccattgg
     5101 gcagttcacc ggtaaacgcc gggaatttat tcttcacgct atagcggcca tcggcctgat
     5161 accaggcaaa tactttacgt acgggctgga caggcagctc cgggagcagg tcttttaccc
     5221 atgttcccgc gcagacaatc gcttttttcg cctgatactc accgtcaacc gtttcaatag
     5281 ttacgccatc atcgtcatga cgaattgcgg tgaccgggca gttgaacagt tgcgcacagc
     5341 ccgcttcctt cgccagttgg atccaggttt taatcgccag ttcgctgcgc aaaaaaccgg
     5401 aatcagtctc aaataagccg atgtagttgt ccgggacgcg tatttctggc cagcgggcca
     5461 taatcccttg cgcatcgagc ttttcaacgt tgagttgcca ctgttcggcg ctgtgggcga
     5521 cgttggcgag aaatgcggag tcagccgggc cgaggttaat gacaccagag cgtacaaaaa
     5581 tgggatcatc ttcgttgtgg cgggaaagtt catcccacag cgtttgcgcg cggaggacca
     5641 acgggacata cttttcgcct tcaccataag catggcgaat taatcgcgta tcgccgtggt
     5701 ggctgccgtg ttgatgcggt ggcatatggg cgtcggtcat tagcacgttt aaaccggcgc
     5761 gggttgcata atacccggcg gcagcgccta cggaaccgct gccaataatg atgagatcgt
     5821 atttcatttt tttctctctg ctctcatggt ttaagcagag taattaactg aatgcagata
     5881 tacaacccag aaatggtaaa ggcaccggtg aggtgccttt tgggtggatg gtcatgtcat
     5941 gttaatgacg ccgatactcg tttaccggga aatcaccgga ttcaattttg gcgattcctg
     6001 cttctaatat cgaaataaat tgtctggcaa catcagtggt taaccagagc gtctgaccaa
     6061 cttcagtccc ttcctgctcg gacttatttg gggtctggta gtgcaaacgc aacatcagcg
     6121 catcatagct atcaacggtg ctgatgtccc accctacgag cggatgagtc tgaatgactt
     6181 cattattttt ttccatcatg gccccctaat tcgtgttact agacaacgtt attcgaggtt
     6241 caatgcgttt ttatctgaag caacttcagt ataccaataa gtaagggtat tcaccgaatt
     6301 tttaaagggg ggaacagata aatttctgct tttaagattt atatgaacaa taaaacagca
     6361 tgtcattcat atttttttag catattgtgc aattattttg aggaagtgta gaaattttgt
     6421 actcaaaatt cgtaagtaaa ataaaaaagc cggggcgatc cggcaaaaac aaaaatcact
     6481 gcatattatt cgctgacaaa ccagtcatcg gcgctttccc acgtttcctg gagaatttcg
     6541 ctaatgcgct gtttatcttc ttttgttgcg ccaataaccg ataaattatt cgctgcggca
     6601 taacgtaccg atacgtggcc ttcattatca ggaaacgcat actgaatacg gcgggaaagt
     6661 tcgccagcca gggcgtcaat agccccagct ggcaatggag aagttttcgc tatggtgact
     6721 tcaattcgca taatagcccc ctgttgaaca tactggttat ttatacaggt aaaataacct
     6781 aatgacaaca ggaagctacg atttttattg tttaacggac cagcgtaccg tttccccggc
     6841 gaggaatggc accagggtgt catcagtcag tgcgatgctt tcagcaacct gttgctcttc
     6901 acgtaccagt tcgatgaatg tgtcgttgac cggcaagcca tagaactgcg ggccgtttac
     6961 agaacagaat gcttcaaagt gctgcagagc attcatctct tcaaagacgg tagcgtaact
     7021 gcccagcgcg gttggggcgt tgaagcagcc cgcgcagccg caactgctct ctttgcgatg
     7081 acgtgcatgt ggcgcagaat ccgtaccgag gaatactcga ttaaaaccgc tggcgaccag
     7141 ttcacgcaat gcctgttggt gaatattgcg tttgaggatg ggtaaacaat acaggtgcgg
     7201 acgcacgcca ccaaccagca tatggttgcg gttaaacatc agatgctgcg gagtgatggt
     7261 ggcagccagc cgttcatttc cgtcacggac atagtcggca gcatctttgg tggtgatgtg
     7321 ctcaaaaacg actttcagcg cagtcaggcg ctggcgcaga ggttccatca cgctttctat
     7381 aaagcgcgct tcacgatcaa aaatgtcgat atctgcatgt gtcacttcac catgcaccag
     7441 tagcggcata ccgatttttt ccatgcgctc aagtaccggc atgattgcgt caattgacgt
     7501 cacgccgtgg ctggagttag tggttgcgtt tgccgggtaa agttttgcag cggtgaacac
     7561 ggcttcatta aatccgcgct ccagctcatt aggatccagc gaatctgtta aataacaggt
     7621 catcaatggg gtgaaatcgt gcccggcagg tacggcgtca agaatacgtt ggcgatacgc
     7681 cacggcagcc tcaactgtgg tcacgggcgg agccagattg ggcattacga tagcccgtcc
     7741 ataaatttcg ctggtatacg gcacgacagt ttttaacatg tcgccatcgc ggaggtgaag
     7801 gtgccagtcg tctgggcggc ggatctttaa tacctgggat ggtgcagtca ttaatctcta
     7861 tgctccggct gaagggatgt ttttgccgga cacaaaggat aagcggaaac gttttccttt
     7921 gcacgaaaaa taaagggcgc gaatgcgccc tcgtcattaa tccgtaaacg gaatgacaat
     7981 ttcgcctggc ttcacttcaa tgcctttcgc cagttttttc gccattgctt cgccctgact
     8041 gccatcttcg cgcaggacgt aagcaggttg ctggttaaag taattgcgta atgcctggtt
     8101 caaataggga agcaacgttt gcatcaccgt ttgcattttt tccggttgta ccgtcgcatc
     8161 gaccacttcc atctctttca ggaagatcgc acctttttct ttatcaaaca cgggcagcgc
     8221 tttcagcttc agtttcatgg tcgctttctg actaccgaac agggagttca tgtccagatt
     8281 ggcgtctccg gttagggtaa ccttattcgg ctcttcgcga ccaatttggc tggtcaagtt
     8341 tgtcagaaca atatgggcgt cagccacgcc gggtaaaccg atatcttttg agaaattatt
     8401 atgtttcgca agcgactggt taatttcttg ttcggtgatg gtgtattggg tgagttgatt
     8461 acagccaacg agcaggccac tgacgatcaa tgcagcggca aataaaaact tgttcatggt
     8521 agtcctcgac atgaaatctg cgtcaatatc ctgacacaac gcagcatgtg tcaccagcga
     8581 taaactcgcc agcagaaaaa actgaaaacg gcggcaaccc gcgaatacag gctgccgcgg
     8641 cgggtcagga ttaaatcgcc attgatgata acaaattgat ttgtgtctgt ttcgccatat
     8701 tatcgcggta attagcaacg cggcttggcc agttgattcc ggctaccagc gtcagattac
     8761 gcagtagcgg aaatagctga atatcatctt ccgaaagttc gccattcacg gcgttcggtt
     8821 tgacgatcag tttgtccagc gcacgcaaat catcgctgat attcttaatc agaccgtcag
     8881 agtgggccag caggtcggca aaattacccg cgctggcctc tttcttgtcg acgaaatatt
     8941 tgcgcgcggc gggagtagaa aactcatcaa atgccgattt ggcaaaacgc ggcaacagca
     9001 gtttgttggc gtagccattg accttgcgca gccactcttc aatggcaggg gaacgtttgc
     9061 cggtcagtaa cggtttgccg tcgagtttat cgacatagtg aacgatatcc atgctttctg
     9121 gcatatagcg gctgtcatct ttttgcagaa tgggaacctg tttttgaccg accatccggg
     9181 tgggtgtttc tgcgtcgtcg ttgagcagaa catgtaattc gacggggata tttttcaggc
     9241 cgaaaatcat gcgggctttg aggcagtaag ggcagtgatc gtaaatgtat agcttcacgt
     9301 gactcctcca tttggctgtc agttcacttt cagtatggag gagtcagggg ggagtatcaa
     9361 atcaggcgtc gcgttccagc aaacgacgcg cggcgcgttt ctgactaaac tgccaaccca
     9421 gcgcaaggaa agtgaagatg ccaataattc ccagcatcat ccacggaagc tctggctggt
     9481 gcgccgattt gcccaggtca aacagccagc caccaccgat ataaccaata gcgccgccaa
     9541 tcgccagacc cagacggcta aaccccatat agctgccgcg agctcttgcg tccgccagcg
     9601 aagcacttaa ggtttcacgc gcaggctcgg caatgatcga cccgatataa aacagacaaa
     9661 tcagggtgaa aagttgttgt aggccgctga ccatgcccac cggcatcatg cttaatgaca
     9721 ttatcaacag cccagccatc aaccggtgtt ccagacgaaa atgcttttca ctccagcggg
     9781 cgatagggta gagcaacgtt aacgacagac acgcttcaat ggcatacatc catttaacgg
     9841 cagagggcgc gccagccacg tcgttgacca taattggcag catcagcatc acttgtacag
     9901 ccagcatgta gtaacccgcc agcgtcagga cataggtgac aaaacgcttg tcacgcatca
     9961 cgcgggtcat gccttcgcga acgggcgtgc gtacggtgga gagtttccat gccggtaaca
    10021 accacgcatt gaacgccgca catagcacaa acaaaacggc ccctgtggcg cagaccaggc
    10081 gaaagtcgta ttgcaacagc cagctcccca acaatgcgcc aatgaccgca ctggcactgt
    10141 cctgcatcat caacagcgag aaaaaacgac cacgttgttg tggacggatt agtttcacca
    10201 ccagcgccga acgcggcgga tcaaacaacg tgccgccgag tcccgagagc aggcatgaaa
    10261 accacaatag ccacggttcg tgggcgatac ccattgtggc gaatccggcg gcgcgcatca
    10321 gcataccggt aacaatcatc ggtttggcac caaagcggtc ggcaattgcg ccgccgaaaa
    10381 tacccagacc ttgctgaata aactggcgta gaccgagagc aataccgacc atgacggcgg
    10441 cccagcccat ttgatcaacg aagcggatag agatcagcgg gaagacaaca aagaacccca
    10501 gcacgaccag catattatcg atgagcagga aatatttacc caggttcctc gcctgcgata
    10561 cgcgggacat ttcccctccc gggaaataaa agatgagcac tttctattct gcattgccgc
    10621 taagactttt cccatcactt cggggacaat atttttttat caaaacgtcg ctttgataga
    10681 gagtttttat caaatatgtg aataatgcag aaaatggcat tttggacttt tcacagggtc
    10741 tggttgcgca ggtatagtaa tacttacagc gtattaaaga cgttacggga aggagtaggt
    10801 atagaatgtt tggctatcgc agtaacgtgc caaaagtgcg cttaaccaca gaccgactgg
    10861 tcgtgcgtct ggtgcatgat cgtgatgcct ggcgtctggc ggattattac gcagagaatc
    10921 gccatttcct caagccctgg gagccagtgc gcgacgaaag ccactgttat ccatcaggct
    10981 ggcaggccag gctagggatg attaacgaat ttcataaaca aggttcagct ttctactttg
    11041 gcttattcga cccggacgaa aaagagatta ttggcgttgc caatttttcc aatgttgttc
    11101 gcggctcttt tcatgcctgc tatctcggtt attcgattgg gcaaaaatgg cagggcaaag
    11161 gactcatgtt tgaagccctg accgcagcca ttcgttatat gcagcgcacc caacatattc
    11221 atcgcattat ggctaattat atgccgcaca ataaacgcag cggtgattta ctggcgcgac
    11281 tgggttttga aaaagaaggc tatgcgaaag actatctgtt gattgatgga caatggcgcg
    11341 atcacgtact gacggcatta actaccccag actggacgcc cggccgctaa ggagaatctc
    11401 atgaaatatc aacttactgc cttggaagct cgcgtaattg gttgcttgct ggaaaagcag
    11461 gtgaccacgc cggagcaata cccgctctca gtcaatggtg tagtcacggc ctgtaatcag
    11521 aaaacgaacc gtaaaccggt catgaatctg agtgaatccg aagtgcagga acagctggat
    11581 aatctggtca aacgtcatta tctacgcaca gtgagcggtt ttggtaatcg ggtcaccaaa
    11641 tatgagcaac gtttttgtaa ttcagaattt ggcgatctga aactgagcgc cgcagaagtg
    11701 gcgttaatca ccacgttgtt attgcgtggt gcccagacgc cgggtgaact gcgcagccgc
    11761 gccgcgcgaa tgtatgaatt cagcgatatg gcggaagtgg agtcgacgct ggaacaactg
    11821 gcgaatcgcg aagatggtcc ttttgtggtg cgtctggccc gcgaaccggg taaacgcgaa
    11881 agccgctaca tgcatctttt cagtggtgag gttgaagatc agccggcggt aacggctatg
    11941 tcgaacatgg ttgacggtga tttatagacc cgcgtcgaag ccctggaaat cgaagtggca
    12001 gaactgaaac aacgtcttga ttcgttgctg gcccatctgg gagattaaag tgaaaaaatt
    12061 acgtatcggc gtagtgggat taggcggcat tgcgcaaaaa gcgtggttac cggtgctggc
    12121 ggcagcgtct gactggacgt tacaaggagc ctggtcgcct acgcgcgcca aagccctgcc
    12181 aatttgtgac agctggcgca ttccttatgc cgattcgtta tccagccttg ccgccagttg
    12241 tgatgcggtt tttgtgcact ccagcaccgc cagccacttt gacgtggtca gtacgttact
    12301 caatgcgggg gtacatgtct gtgtcgataa accgctggca gaaaatctgc gcgatgctga
    12361 aaggctggtg gaactggcgg cgcggaaaaa actgacgttg atggtcggtt ttaaccgtcg
    12421 tttcgcacca ctctacggtg agttaaaaac gcaactcgcc accgcagcct cgctaagaat
    12481 ggataaacat cgtagcaata gcgtcgggcc acacgatctt tatttcacgt tgctggatga
    12541 ttatctgcat gtggtggata ccgcgctgtg gttgtcgggc ggcaaagcct ctctggatgg
    12601 cggtacgcta ctgactaacg acgctggcga aatgctgttt gccgagcacc atttttcggc
    12661 cggtcctttg cagatcacca cttgtatgca tcgccgtgcc ggaagtcagc gtgaaaccgt
    12721 gcaggccgtg actgacggtg cgctcatcga cattacggat atgcgcgaat ggcgtgagga
    12781 gcgcgggcag ggtgtagtga ataaaccgat tcctggttgg cagagcactc ttgagcaacg
    12841 tggatttgtc ggctgtgcgc ggcacttcat tgaatgcgtg caaaatcaga cagttccgca
    12901 aaccgccggc gaataggcag tgctggcgca acgtatcgtt gacaagatct ggcgcgatgc
    12961 gatgagtgaa taaaccctgt aacatctggc ggtagcaatt caccataatc taggtagact
    13021 attcgcctct ttcagcgcct gccttgcagg cgttttgccc gtgggtccgg attagaacac
    13081 cgatgaattt attaaaatcg ctggccgccg tcagctcgat gaccatgttt tcgcgtgtgc
    13141 ttggcttcgc acgagacgca attgtcgcca gaatctttgg cgcagggatg gcaaccgacg
    13201 cctttttcgt cgcttttaaa cttcctaact tgttacgccg tatctttgcc gaaggggcat
    13261 tttcccaggc atttgtaccg attctggcgg aatataaaag taagcagggt gaagacgcca
    13321 cgcgggtctt tgtctcttat gtttctggcc tgctgacact tgcgctggcg gttgtgacgg
    13381 tcgctggcat gctcgccgca ccgtgggtga tcatggtgac cgcgccaggc ttcgctgaca
    13441 cagctgacaa atttgccctg accagccagc tactaaagat tacctttccc tatatcttgc
    13501 tgatctccct ggcgtcgctg gtgggagcga ttctgaatac gtggaaccgc ttctcgattc
    13561 cggcgtttgc gccaacactg cttaacatca gcatgattgg tttcgcgctg tttgccgcac
    13621 cgtactttaa cccaccggtg ctggcgctgg cgtgggctgt tacggtcggc ggcatcctgc
    13681 agctggtgta tcagctaccg cacctgaaga agatcggcat gctggtcctg ccgcgcatta
    13741 acttccacga tgctggagca atgcgcgtgg tgaaacagat gggaccggcg atccttggcg
    13801 tctctgtgag ccagatctcc ttaatcatca acaccatttt tgcctcgttt cttgcttccg
    13861 gttcggtgtc ttggatgtat tacgccgacc gcttaatgga gtttccctcc ggtgtgctgg
    13921 gcgtggcgct tggcaccatt ttgctgccgt cgctgtcgaa aagttttgcc agtggcaatc
    13981 atgatgaata caaccgtttg atggactggg ggttgcgtct ttgtttcctg ttggcactgc
    14041 cgagtgcggt tgcgttgggc attctttcag gtccgttaac cgtttcgctg ttccagtacg
    14101 gtaaatttac cgcgtttgat gcgctgatga cccagcgggc gttaattgcc tactcggtgg
    14161 gtttgattgg cctgattgta gtgaaagtgt tggctcctgg cttttattcc cgccaggaca
    14221 ttaaaacgcc agtgaaaatt gccatcgtta cgctgatttt aacgcaattg atgaacctgg
    14281 cgtttattgg tccgttgaaa catgccgggc tgtcactttc tattggtctg gcggcgtgtc
    14341 tgaatgcttc gctgctttac tggcagttgc gtaagcagaa aatctttact ccgcaacccg
    14401 gctggatggc gtttctgttg cgtctggtgg tggcggtact ggtgatgtct ggcgtgcttt
    14461 taggtatgtt acatatcatg ccggagtggt cattgggtac catgccctgg cgcttactgc
    14521 gtttaatggc ggtcgtgctg gcggggattg ccgcgtactt cgctgcactg gcggtactgg
    14581 gcttcaaagt taaagaattt gcccgccgga cggtgtaaca atgcattccg gcctgcagtg
    14641 caggccggag ataatattca gatcgaaatc tttttactgc cgcgatgggg tgttgaggtc
    14701 tgaccgttcg ccccatatag cgtcggttcc tgatgcggtt tcaacatttc cagcgcctgt
    14761 tgattgcgct caatctgtcc ttccagtaac cagccgttat gttgattcat ttggcgtagt
    14821 tgctgcgttt tcacagtaat ttcctgccag cgttgactaa tatcaacgct atttgctgta
    14881 ttgggttctt tcttgcgtaa ctgttcgagg taatccagcg tcgccagcag tgagcttttt
    14941 tgttctgtaa tccattgcaa ctggctgccg ttgatctgcc ccatagagag atgttgctgc
    15001 tcttgatcca ttaccgtttt gagatcgtta agcacagcgg acatctggtc gaggatctct
    15061 gcaagacgtg tcatacgatc agttactctg caagtcttgc tgcgcttcgt tgatcagcgc
    15121 atcggcaatt ttgccggtgt ccatttttag ttcaccgtta cgaatcgcca gttttaacgc
    15181 ttcgacacgt tcaagattga tatcactgct gccgggttgc atcagttttg cttgcgcgtc
    15241 gcttaacgtc acactggtgc tggtggaggc ggttgttttt gccgcccggc tgttcgttac
    15301 cggcgcgtcg gtggtttcgc gcggttgaac ggtgcttaca ggcttcagag gcgaagtgcg
    15361 atcaatactc atggtttatt cctcattgag ggcgctttta tcatgtgttg cttatttatc
    15421 ggcaagggac gggtaatctt taacagctta caggtttata agaatattcc catctgcatc
    15481 aacaacgccg ctgactacct gtcccgatac catgcgcacc cgcgcattct gtgcgacggc
    15541 tgcattgttc agcgcctgac cttctgcgtt ggcgctaaac ccatcaccgc tggcgatcac
    15601 attgacgcgt tgtcccgctt ttacccgcca tgcctggcga aactgggtta actggatagg
    15661 ttgatcgggt gatagatcgc gcaggctaac ggcatcaaca agttgattga tatccagcac
    15721 cgtacgcggt ggcagggtat ccaggcgtcc acgtttcagt ttgacattgc cagcttccag
    15781 ctttcctccc cgcacaatag gcatcgcggc aaccacataa tttcctgtgg cctgcacatt
    15841 aacctgtaaa tatcgtttgt cgttaccgca gcgtgccaac acattcacat tgccccacag
    15901 gcgggaatta ttgctcatcg aaagcaatgg ctgctcgcat ggcggtagta gattgggcgc
    15961 tgtacgaata gaaacacgaa cctcatcact tacccccgcg agttgggcgc taaaaaagtt
    16021 gtgcaattgc gatgtgagat tgctcgccgt acttaacgga ctgaacagta tcgcgatgat
    16081 cgccacgcta cgttttattg tcagcatttt cgcccccagc catttctaca acgtgaattg
    16141 tacctgtccg caatgaccat caacggcata aatagcgacc cattttgcgt ttattccgcc
    16201 gataacgcgc gcgtaaaggc atttaagctg atggcagaat tttgatacct gcggaggaga
    16261 tatgctcgat aagctcgacg ccgccttacg ttttcaacaa gaggcgctca atctgcgcgc
    16321 ccagcgtcag gaagtgctgg cagcaaacat cgccaatgcc gatacccctg gttatcaggc
    16381 gcgcgatatc gattttgcca gtgaacttaa aaaagtcatg caacgtggac gggatgcaac
    16441 cagtgtggtt gcactgacga tgacctcaac gcaacacatc ccggcgcagg cgctgacgcc
    16501 tcctaccgca gaactgcaat accgtattcc ggaccagcct tcgcttgacg gtaataccgt
    16561 cgatatggat cgcgaacgca cccagtttgc cgataacagc ctgcaatacc agatgagcct
    16621 tagcgcgttg agcgggcaaa tcaaaggcat gatgaacgtt ttacagagcg gaaattaacg
    16681 gatggcactg ctgaatattt ttgatatcgc cgggtcggcg ttaactgccc agtcccagcg
    16741 cctgaacgtg gcggccagta atctggcaaa tgctgatagc gtgaccggtc ctgatggaca
    16801 gccatatcgg gcaaaacagg tggtattcca ggttaacgct gcaccaggtg ctgcgacagg
    16861 cggcgtaaag gttgccgatg ttaagaaagt caggccccgg acaaactggt ttatgaacca
    16921 ggtaatccgc tggcagatgc aaagggctac gtaaaaatgc cgaacgttga tgtcgtcggg
    16981 gagatggtta acaccatgtc ggcgtcacgc agctatcagg ccaatgttga agtgctcaac
    17041 acggtgaaaa gcatgatgct gaaaaccctt acgctcggtc aataaaggag aaagctatgt
    17101 ccattgcggt aaccaccacc gatccgacaa ataccggcgt cagtaccacc agcagtagtt
    17161 cgctcacggg cagcaacgcc gcagatttac aaagcagttt tctgactttg ctggtggcgc
    17221 agctgaaaaa ccaggacccg accaatccaa tggaaaacaa cgagctgacg tcgcaattgg
    17281 cacaaatcag cacggtcagc gggattgaaa aactcaatac cacgctcgga tctatttccg
    17341 gacagattga taacagccag tcgttacagg ccagtaacct gatcggtcac ggcgtgatga
    17401 tccccggcac aactgttctt gcgggaaccg gcagtgaaga aggggctgtg accacgacca
    17461 cgccgtttgg tgttgagctg caacaggcgg cagacaaagt tacggccacc atcaccgata
    17521 aaaatggcgc ggttgtgcgc accattgata ttggtgaact gaccgccgga gttcacagtt
    17581 tcacctggga cggttcgttg actgatggca gcactgcgcc gaacggttct tacaatgtag
    17641 cgattagcgc cagtaacggt ggtacacaac tggttgccca gccgctgcag tttgctctgg
    17701 tgcagggtgt gatccgcggc aacagcggta atacgctgga tctcggcact tacggcacca
    17761 ccaccctcga cgaagtacgg cagataattt aagccttcac atttcaggag tcagtcatgg
    17821 ccttttctca agcggttagc ggattaaacg ctgccgccac caacctcgat gttattggca
    17881 acaatatcgc caactccgcc acctacggct ttaaatcagg cacggcctct tttgccgata
    17941 tgtttgccgg ttcgaaagtg ggactggggg taaaagttgc cggtatcact caggacttta
    18001 ccgatggcac gaccaccaac accgggcgag gtctggacgt tgctatcagc cagaacggtt
    18061 ttttccgtct ggtagacagc aacggttcgg tgttctacag ccgtaacgga caatttaagc
    18121 tggatgaaaa ccgtaacctg ttgaatacgc aaggtttaca gctgacgggt tacccggtaa
    18181 ccggtacgcc gccgactatt cagcaagggg cgaatccgac caatatttcg atcccgaata
    18241 ccctgatggc agcgaaaact accaccacgg cgtcgatgca gatcaacctg aattccagtg
    18301 atccgcttcc tactgttacg ccattcagcg ccagcaatgc ggatagctat aacaaaaaag
    18361 gttcggtgac tgttttcgac agtcagggta atgctcatga catgagcgtc tattttgtga
    18421 agaccgggga taataactgg caggtctaca cccaggatag cagtgatcca aacagcattg
    18481 cgaagacagc gacaacactg gaatttaatg ctaatggcac attagtggat ggtgcgatgg
    18541 cgaataatat cgcaaccggc gcaattaacg gtgcagaacc cgccacgttt agtctgagct
    18601 tcctcaactc catgcagcaa aataccggcg ctaacaacat tgtggcaacc acccagaatg
    18661 gctacaaacc gggcgatctg gtgagttatc aaatcaatga tgacggtacg gttgtcggca
    18721 acaattccaa cgaacaaacc caactgctgg ggcagattgt actggcgaac tttgccaaca
    18781 acgaaggtct ggcatccgaa ggcgacaacg tctggtctgc gacgcaatct tctggcgtgg
    18841 cgctgttggg gacagccggg acgggaaact ttggcaccct gaccaacggt gcgctggaag
    18901 cgtccaacgt cgatctcagt aaagaactgg tcaatatgat cgttgcccag cgtaactata
    18961 agtctaacgc ccagaccatc aaaacccagg accagatcct caacacgcgg gttaacttac
    19021 gctaatcgct gacgggatag ctcaatggat cacgcaattt atactgcgat gggagcagcc
    19081 agccagacgc tgaatcaaca ggcggtaacc gccagtaatc tggccaatgc ctctacgccc
    19141 ggttttcgcg cgcagttaaa cgccttgcgc gcagtgccgg tggaagggct ttcactgccc
    19201 acgcgcacgc tggtaacggc gtcaacaccg ggtgcagaca tgacgcccgg caagatggat
    19261 tacacctcgc gcccgctgga cgttgcgttg cagcaggatg gctggccgtg cagaccgctg
    19321 acggcagcga agggtatacg cgtaatggca gcattcaggt tgatcccacc gggcaactga
    19381 caattcaggg gcatccggtg ataggcgagg cggggccaat tgctgtgccg gaaggggcgg
    19441 aaatcacgat tgctgccgat ggcacaatct cggcgctcaa tccgggcgat ccggcaaata
    19501 cagttgcgcc agtagggcgt ctgaaactgg tgaaagccac gggcagcgaa gtgcagcgcg
    19561 gtgacgacgg catttttcgt ttaagcgcag aaacccaggc cacgcgaggg ccggtactgc
    19621 aggcagatcc aaccttgcgt gtgatgtcgg gggttctgga aggcagtaac gtcaatgccg
    19681 ttgcggcaat gagcgacatg attgccagcg cgcggcgttt tgaaatgcag atgaaggtga
    19741 tcagcagcgt cgatgataac gcaggccgtg ccaaccaact gctgtcgatg agttaattga
    19801 acgattgatg acattgcaat aaaagcgcgc ggactattcc ctcgcccctt tggggagagg
    19861 gttagggtga ggggaaattc acactgtcgc gtgcttctgc tctgcttgtc agcaatccac
    19921 tacaggacat tatatgatca gttcattatg gatcgccaaa acgggccttg acgcccagca
    19981 aaccaatatg gacgtcattg ccaacaacct ggcaaacgtc agtactaacg gttttaagcg
    20041 tcagcgcgcg gtgtttgaag atctgcttta tcaaaccatt cgccagccgg gggcacagtc
    20101 ttccgaacaa accaccttac cctccggatt acaaatcggc acgggggtac gcccggtcgc
    20161 cactgaacgc ttacacagcc agggcaatct gtcgcagacc aataacagca aagatgtcgc
    20221 gattaaaggg cagggctttt tccaggtaat gttgccagat ggctcatcag cctatatccg
    20281 tgacggctct ttccaggtgg atcagaacgg gcagctggtg acggctggtg gttttcaggt
    20341 gcagccagcg atcaccattc cggcgaatgc gttaagtatc accatcggtc gtgatggcgt
    20401 ggtcagcgta acccaacaag gccaggcagc tccggttcag gttgggcagc tcaatctcac
    20461 cacctttatg aatgataccg gactggagag cattggcgaa aacctctaca ccgaaacgca
    20521 atcctccggt gcaccgaacg aaagcacgcc gggcctgaac ggcgcgggac tgctgtatca
    20581 agggtatgtt gaaacgtcta acgtcaacgt ggcggaagaa ctggtcaata tgattcaggt
    20641 gcaacgcgct tacgaaatca acagtaaagc ggtgtccacc accgatcaga tgctgcaaaa
    20701 actgacgcaa ctctaaggca taaccggtgg caggttcacc ggtttactga tttttgaaga
    20761 tgatagccat gcaaaaaaac gctgcgcata cttatgccat ttccagcttg ttggtgcttt
    20821 cactaaccgg ctgcgcctgg ataccctcca cgccgctggt gcagggggcg accagtgcac
    20881 aaccggttcc cggtccgacg cccgtcgcca acggttctat tttccagtct gctcagccga
    20941 ttaactatgg ctatcaaccg ctgtttgaag atcgtcgacc acgcaatatt ggcgatacgc
    21001 tgaccatcgt gttgcaggag aacgtcagcg ccagcaaaag ctcctctgcg aatgccagcc
    21061 gtgacggtaa aactaatttt ggctttgata ctgtgccgcg ctatttgcag gggctgtttg
    21121 gtaacgctcg tgccgatgtc gaagcctccg gtggtaacac gttcaacgga aagggcgggg
    21181 ccaatgccag caataccttt agcggcacgt tgacggtgac ggttgaccag gtactggtca
    21241 acggcaacct gcatgtggtg ggtgaaaaac agattgccat taatcagggt accgaattta
    21301 ttcgcttctc gggcgtggtt aatccacgca ctatcagcgg cagcaatacc gtaccgtcta
    21361 ctcaggtggc ggatgcgcgt attgaatacg taggcaatgg ctacattaac gaagcgcaaa
    21421 atatgggctg gttgcagcgt ttcttcctta acctgtcgcc aatgtaagtg aggctgtggt
    21481 gattaaattt ctctctgcat taattcttct actggttacg acggcggctc aggctgagcg
    21541 tattcgcgat ctcaccagtg ttcagggggt aaggcaaaac tcactgattg gctatggctt
    21601 ggtagtgggg ctggatggca ctggtgacca gacaacccag acgccgttta ccacacaaac
    21661 gcttaataac atgctctcac agctgggaat taccgttccg acgggcacca atatgcagct
    21721 aaaaaacgtc gctgcggtaa tggtgacagc gtcacttcct ccgtttggac gtcaggggca
    21781 aaccatcgat gtggtggttt cttccatggg taatgccaaa agcttgcgtg gaggtacgtt
    21841 gttgatgaca ccgcttaagg gcgttgacag tcaggtgtat gcgctggcgc agggcaatat
    21901 tctggttggc ggcgcaggag cctccgcagg cggtagcagt gttcaggtta accaactgaa
    21961 cggtggacgg atcaccaatg gtgcggttat tgaacgtgaa ttgcccagcc agtttggcgt
    22021 cgggaatacc cttaatttgc aacttaacga cgaagatttc agcatggcgc agcaaatcgc
    22081 tgacaccatc aaccgcgtgc gtggatatgg cagcgccacc gcgttagatg cgcggactat
    22141 tcaggtgcgc gtaccaagtg gcaacagttc ccaggtccgt ttccttgccg atatccagaa
    22201 tatgcaggtt aatgtcac
//
LOCUS       DAJVRP010000065        22102 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVRP010000065 DAJVRP010000000
VERSION     DAJVRP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.065"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..407)
                     /gene="sxy"
                     /locus_tag="OQN12_002578"
     CDS             complement(<1..407)
                     /gene="sxy"
                     /locus_tag="OQN12_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS1654851.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            626..1135
                     /gene="sulA"
                     /locus_tag="OQN12_002579"
     CDS             626..1135
                     /gene="sulA"
                     /locus_tag="OQN12_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1654852.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1491..2537
                     /gene="ompA"
                     /locus_tag="OQN12_002580"
     CDS             1491..2537
                     /gene="ompA"
                     /locus_tag="OQN12_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1654853.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2613..3065)
                     /gene="matP"
                     /locus_tag="OQN12_002581"
     CDS             complement(2613..3065)
                     /gene="matP"
                     /locus_tag="OQN12_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1654854.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3250..5010
                     /locus_tag="OQN12_002582"
     CDS             3250..5010
                     /locus_tag="OQN12_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1654855.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEIASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5079..5597
                     /gene="fabA"
                     /locus_tag="OQN12_002583"
     CDS             5079..5597
                     /gene="fabA"
                     /locus_tag="OQN12_002583"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1654856.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5667..5834)
                     /gene="rmf"
                     /locus_tag="OQN12_002584"
     CDS             complement(5667..5834)
                     /gene="rmf"
                     /locus_tag="OQN12_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1654857.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6090..6653)
                     /gene="pqiC"
                     /locus_tag="OQN12_002585"
     CDS             complement(6090..6653)
                     /gene="pqiC"
                     /locus_tag="OQN12_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1654858.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6650..8290)
                     /gene="pqiB"
                     /locus_tag="OQN12_002586"
     CDS             complement(6650..8290)
                     /gene="pqiB"
                     /locus_tag="OQN12_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1654859.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8295..9548)
                     /gene="pqiA"
                     /locus_tag="OQN12_002587"
     CDS             complement(8295..9548)
                     /gene="pqiA"
                     /locus_tag="OQN12_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1654860.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9678..11585)
                     /locus_tag="OQN12_002588"
     CDS             complement(9678..11585)
                     /locus_tag="OQN12_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1654861.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11597..13705)
                     /gene="rlmKL"
                     /locus_tag="OQN12_002589"
     CDS             complement(11597..13705)
                     /gene="rlmKL"
                     /locus_tag="OQN12_002589"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1654862.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13949..15058
                     /gene="ycbX"
                     /locus_tag="OQN12_002590"
     CDS             13949..15058
                     /gene="ycbX"
                     /locus_tag="OQN12_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1654863.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15055..15597)
                     /gene="zapC"
                     /locus_tag="OQN12_002591"
     CDS             complement(15055..15597)
                     /gene="zapC"
                     /locus_tag="OQN12_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1654864.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15771..16781)
                     /gene="pyrD"
                     /locus_tag="OQN12_002592"
     CDS             complement(15771..16781)
                     /gene="pyrD"
                     /locus_tag="OQN12_002592"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1654865.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16892..17631)
                     /locus_tag="OQN12_002593"
                     /pseudo
     CDS             complement(16892..17631)
                     /locus_tag="OQN12_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17597..18112)
                     /locus_tag="OQN12_002594"
     CDS             complement(17597..18112)
                     /locus_tag="OQN12_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1654866.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18120..18662)
                     /locus_tag="OQN12_002595"
     CDS             complement(18120..18662)
                     /locus_tag="OQN12_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1654867.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18760..19743)
                     /locus_tag="OQN12_002596"
     CDS             complement(18760..19743)
                     /locus_tag="OQN12_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1654868.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19734..>22102)
                     /locus_tag="OQN12_002597"
     CDS             complement(19734..>22102)
                     /locus_tag="OQN12_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCS1654869.1"
                     /translation="LNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAFPAF
                     KQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGINAL
                     FSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWKNLSSYLQ
                     RAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVRGIAKSNA
                     QITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSVPFSSVPL
                     LQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAEYYRAAMF
                     GLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQLMGYRYST
                     SGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQLGEYGLF
                     YLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVFALNFSLP
                     LNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSYSVQQGYN
                     SEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGITLGQSLG
                     ETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKRNVDLEN
                     AVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDDG
                     SLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
BASE COUNT         5478 a         5792 c         5193 g         5639 t
ORIGIN      
        1 acctccccga gaatcgcttc cagatgaaaa gacatattgg gcaaatcttt cagtctttcc
       61 cgggtattgc gcgtgctttt ctctttcagc gctgcatcga gagaatattt cgacagacgc
      121 accagcttca gttgatttcg ccatagactt tcatcaaccc gatagtaatt gagggtaacg
      181 gatcggccac actttttata tgtcagccag acaggcggat gttttacaca gtactgtgca
      241 ctttgctcac aagcccgaag atacaactca ccatcagaaa ccatcgcaaa caccgtgtcg
      301 tcaacggtca ggctgtaact gccaaacaat gatcggtatt caattgtgcc caacgttgcc
      361 aggtattctt gtgatttata gatccgctta taggagaggc ttttcataaa attcctttta
      421 aaatcataac ataaaagaat gattcacatt aacggatccg ttaactacga aaataggcaa
      481 cttattctta aggggcaaga ttaatttatg ttttcccgtc accaacgaca aaatttgcga
      541 ggctctttcc gaaaataggg ttgatctttg ttgtcactgg atgtactgca catccataca
      601 gtaactcaca ggggctggat tgattatgta cacttcaggc tatgcacatc gttcttcgtc
      661 gttctcatcc gcagcaagta aaattgcgcg tgtctctacg gaaaacacta cagccgggct
      721 tatcagtgaa gttgtctatc gcgaagatca gcccatgatg acgcaacttc tactgttgcc
      781 attgttacag caactcggtc agcaatcgcg ctggcaactc tggttaacac cgcaacaaaa
      841 actgagtcgg gaatgggttc aggcatctgg gctaccctta acgaaagtaa tgcagattag
      901 ccagctctcc ccttgccaca cagtggagtc aatggttcgc gctttacgca cgggcaatta
      961 cagtgtggtg atcggttggt tgacagatga tttgactgaa gaagagcatg ctgaacttgt
     1021 tgatgcggca aatgaaggta acgctatggg gtttattatg cgtccggtaa gcgcatcctc
     1081 tcacacaacg agacaacttt ccgggctaaa aattcactct aatttgtatc attaagtaaa
     1141 tttaggatta atcctagaac tttttttgtc gcccagccaa tgctttcagt cgtgactaat
     1201 tttccttgcg aaggcttgtc tgaagcggtt tccgcgtttt ttttctgtaa attgtcgctg
     1261 acaaaaagat taaacatacc ttatacaaga cttttttttc atatgcctga cggagttcac
     1321 acttgtaagt tttcaactac gttgtagact ttacatcgcc aggggtgctc ggcataagcc
     1381 gaagatatcg gtagagttaa tattgagcag atcccccggt gaaggattta accgtgttat
     1441 ctcgttggag atattcatgg cgtattttgg atgataacga ggcgcaaaaa atgaaaaaga
     1501 cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag gccgctccga
     1561 aagataacac ctggtacact ggtgctaaac tgggctggtc ccagtaccat gacactggtt
     1621 ttattcctaa caatggtccg acccacgaaa accaactggg tgcaggtgct tttggtggtt
     1681 accaggttaa cccgtatgtt ggttttgaaa tgggttacga ctggttaggt cgtatgccgt
     1741 acaaaggcga caacatcaac ggcgcataca aagctcaggg cgttcagctg accgctaaac
     1801 tgggttaccc aatcactgac gatctggaca tctacactcg tctgggtggt atggtatggc
     1861 gtgcagacac caaggctaac gtacctggtg gcgcatcctt taaagaccac gacactggcg
     1921 tttctccggt tttcgcaggt ggtgttgagt acgcgatcac tcctgaaatc gctacccgtc
     1981 tggaatacca gtggaccaac aacatcggtg acgcaaacac catcggtact cgtccggaca
     2041 acggtctgct gagcctgggt gtttcctacc gtttcggtca gggcgaagca gctccggtag
     2101 ttgctccagc tccggcaccg gaagtacaga ccaagcactt cactctgaag tctgacgttc
     2161 tgttcaactt caacaaagca accctgaaac cggaaggtca ggctgctctg gatcagctgt
     2221 acagccagct gagcaaccta gatccgaaag acggttccgt agttgttctg ggttacactg
     2281 accgcatcgg ttctgacgct tacaaccagg gtctgtccga gcgccgtgct cagtctgttg
     2341 ttgattacct gatctccaaa ggtatcccgg cagacaagat ctccgcacgt ggtatgggcg
     2401 aatccaaccc ggttactggc aacacctgtg acaacgtgaa acagcgtgct gcactgatcg
     2461 actgcttggc tccggatcgt cgcgtagaga tcgaagttaa aggtatcaaa gacgttgtaa
     2521 ctcagccgca ggcttaagtt atcgtctggt agaaaaaccc cgctgctgcg gggttttttt
     2581 tgcctttagt aaattgaact gactttcgtc agttattcct tacccagcaa tgcctgcaga
     2641 tcctgcttaa gagaagacat tttattcgcg tatttctctt tgttttccgc atcttcaatc
     2701 agctgaacaa tcgtttcaga aagcgtttta ccgcgacgct gtgcaagacc agccagacgt
     2761 tgccagacaa taaattccag atcgatcgat tttttgcgcg tatgctggtg ttccgcatta
     2821 aaatggcgct tccgtctggc ccgaatggtc tgcttcatgc gattgaccag ttccggattc
     2881 atatgcttgt caatccagcc atttaccagc acgggttcat tttcgagcga gagcaacaca
     2941 tcgacggctt cctgggcggc actggcttct atgtaacggg taattaactc cccttcgcga
     3001 tgcttcttca ccagatactt ccatttccaa ccgctttcaa gattttcaag ttgttgatat
     3061 ttcatagcga tctcaatgtt accgtgtaac tctttacaga atatcagctt tttaggccag
     3121 tgaagaaaag aatctccatc ctgtgagctt taacgcccag ccaggagtat tgtcggtgct
     3181 ttctacgtgt gctgtagtgc cggttacggt ataatcgcgg ctttgacaac agactaaaaa
     3241 acatcaactt tgaccattac gaaacttgca tggcgtgacc tggttcctga taccgatagc
     3301 tatcaggaaa tatttgctca gccacatttg attgacgaaa acgatccttt attcagtgat
     3361 actcaaccgc gactgcaatt tgcgctggag cagttgctgc atacgcgagc atcctcctct
     3421 tttatgctgg ctaaggcccc ggaagagtct gagtatctga atcttattgc cgatgccgcg
     3481 cgtacgctac aaagcgatgc aggccaactg gtgggcgatc actatgaggt ttccggccac
     3541 tccatccgct tacgtcacgc agtgagtgca gatgataatt ttgcgacttt aacgcaagtt
     3601 gtcgctgccg actgggtaga agcggagcaa ctctttggct gcctgcgcca gtttaatggc
     3661 gacattaccc tgcagcctgg tctggtgcat caggcaaatg gcggtattct catcatctct
     3721 ttgcgtacac tgctggcgca acctctgctg tggatgcggc tgaaaaatat cgttaaccgc
     3781 gagcgttttg actgggttgc gtttgatgag tcgcgccctc tccccgtctc tgtgccttcg
     3841 atgccattga agctgaaagt cattctggta ggcgaacgcg aatcattggc tgatttccag
     3901 gagatggagc cagagctttc agagcaggct atttatagcg aatttgaaga tactctgcag
     3961 attgtcgatg cggagtcagt aagccagtgg tgtcgctggg tgacatttac cgccagacat
     4021 aatcacttac ctgccccggg agcggatgct tggccggtac ttatccgcga agcagcacgc
     4081 tacaccggtg aacaagaaac acttccgctt agcccgcagt ggatcctccg ccagtgtaaa
     4141 gagatcgcct ccctgtgtga tggcgacacc ttctccggcg agcagctaaa cttaatgctg
     4201 cagcagcgtg aatggcgcga aggtttcctc gctgaacgta tgcaggatga gatccttcag
     4261 gagcaaatcc tgattgaaac cgaaggcgaa cgcatcgggc aaattaacgc cctttcggtc
     4321 attgaatttc cgggtcatcc acgcgctttt ggcgaacctt ctcgcattag ctgcgttgtg
     4381 catattggcg atggtgaatt caccgacatc gaacgcaaag cggagcttgg cggcaatatc
     4441 catgcgaaag ggatgatgat catgcaggcg ttcctgatgt cggaactaca gcttgagcaa
     4501 cagatcccct tctcagcatc gctgacattt gagcagtcat acagtgaagt ggatggcgat
     4561 agtgcctcga tggctgaact ctgcgccctg ataagcgccc tcgctgatgt gccagtgaat
     4621 cagagtatcg ctatcacagg ttcagtcgat cagttcggcc gcgtccagcc ggtcggtggt
     4681 ttaaatgaga aaatcgaagg cttctttgct atttgccagc aacgtgagtt aacggggaaa
     4741 caaggtgtca ttatccccac tgctaacgtt cgccatttaa gtcttcacag tgaactggtg
     4801 aaagcggtag aagaaggcaa attcaccatc tgggcagtag acgatgtgac tgacgcactg
     4861 ccgttattat taaatctggt gtgggatggc gaaggccaaa cgacgctgat gcaaaccatc
     4921 caggaacgta tcgcgcaagc atcgcaacag gaaggacgtc accgttttcc atggccatta
     4981 cgttggctga actggtttat tccgaactga tcggacttgt tcagcgtaca cgtgttagct
     5041 atcctgcgtg cttcaataaa ataaggctta cagagaacat ggtagataaa cgcgaatcct
     5101 atacaaaaga agaccttctt gcctctggtc acggtgaact gtttggcgct aaaggcccgc
     5161 aattgccagc accgaacatg ctgatgatag accgtgtggt caaaatgacc gaaacgggtg
     5221 gtaacttcga caaagggtat gttgaagcag aactggatat caatccggat ctgtggttct
     5281 tcggatgcca cttcattggc gatccggtta tgccgggatg cctgggcctg gacgcaatgt
     5341 ggcagctggt agggttctac ctcggctggc tgggcggcga aggtaaaggc cgcgcgctgg
     5401 gcgttggcga agtgaaattc actggtcagg tactgccgac agcgaaaaaa gtgacctacc
     5461 gtattcactt taaacgcatt gttaatcgtc gtctgattat gggtctggca gatggcgaag
     5521 tgctggttga ttgtcgtctg atctataccg ccagcgacct gaaagtcggt ctgttccagg
     5581 atacgtctgc cttctgatta tcatcagaca gagtatcaaa aggcgaaacc tccgcaatgc
     5641 ggaggtttct ttttaaagag acagaatcag gccattacta ccctgtccgc catggcttct
     5701 cgccagcctc ccagccattg tgacctttga ctcagcgtct gatagggaca catttctttt
     5761 gagcgtccgg cgatgccggc ctgataacca cgttgatgtg cccgttccag gcgatctcgt
     5821 ttttgtctct tcatgcctcg tttccctcat actggtttct ggtggaaaag aaaacagtga
     5881 ttactaaatg tgcaatcaca gttaagcaat accgcgaatc ataactgccg tcaatgcgca
     5941 aaattcacgc cactgtcata tttgtgagct acacgggcat gtcggttgta caaaaaatgt
     6001 gaagaggaac aaatttttcc gcacgttaga aacaaaaaaa ccgcctcagg caacataccg
     6061 gagacggtta tatttacaaa tctttataat taaggtagac gctttatctc ttgtgcaata
     6121 gaagcggctt cctgactcca gacaccggcc agcactttaa ccatctcatc gtaaccatcc
     6181 tgagtttgca ctccttccag acggaacgga cgtttgatca gttgtccctg gtggttcaac
     6241 agccactcac cactgacaat gaccttgcca tcatagcgac cgttaaactc cgttacggta
     6301 acattgagcg tgtcctgggc gcttcccaga ggctgggagg caaccaccca gccgggcagt
     6361 tgcgtactca ggttggcaac cagggtgttg cgcaactgtt gatccaacgg gctggcccac
     6421 aagttgttgt tggcaatcac atacttcaca tcactggttt gataaaccac accattcccc
     6481 gccagatagt caggaacagt gacctgctct acccataaca gacgattgcc ctggctggcg
     6541 gtactttgtg taccgctctg caccacaggt aactggtaat agtttttatt aatttcgccg
     6601 gagctgcatc cggccagcca cagtgctgca atcgtcacta gccacttttt cattgtttcg
     6661 ccctcttcgg ctctggatct tttttgtcct tcgcttcaaa taccagcgcg ttactcttct
     6721 cattgagcgt tttcagcacc ggttgcagtt ctcgcaacac ctgatcaagg cgctgcatat
     6781 ccgccaccat cttgttgtag gctgcggagc caggctggaa gccctgcatg ctgcgattca
     6841 attcacgcaa cgttgactgc atatccgtcg gcaactgctg catcgactgg ctggcgagga
     6901 tcttgttcat gctatccagc gtcgtttgca ggtttttcat tgtgcgctga ctttcagaaa
     6961 gcgtactggt tgcctgttca atcatcggat tcaatggcag tttgttgatc ttatccaacg
     7021 cttccatcag tcgttgctgg atttgcgcca agccgccgct aacagtcggg ataatctgat
     7081 aaccattaaa ttcacgaata ccggttattg caggcgtatt tggatagaaa tcgagatcaa
     7141 catacagtgc accagtgacc aggtttccgg ttttcagcga tccgcgtaaa ccacgtttca
     7201 acaattcgcc aaggtgctca acaacatccg cattttcgcc aagctgcatt ttcagccgct
     7261 ctggctcgat acgaatcagt accggaatac ggtaatcatc gttaaatgtc tgacgcatat
     7321 tcggcgcaaa gaatggcact ttgcttacgg tacccaggcg aataccccgg aattctaccg
     7381 gagctcccgg ttgcagaccg cgtaccgaat ctttaaagaa catcagataa tcaatgtgat
     7441 cggtgtacaa cgaatcctga atgctcttct gatcatcata caaaacgaaa gctgttttcg
     7501 gtgccactgg ctgccctaaa tccagacctt ccggcacatc aaagctaaca ccgccactaa
     7561 gcagcgttgt caatgagccc atctccacac gcatccccgc tgacgtcaga tcaaccgcaa
     7621 tgccactatc tttccagaag cgaacattgc tggtcaccag tcggtcataa ggtgcattga
     7681 tgaacagttg ataactgata ttgcgttttt gcgtatcgaa ggtgctggtt tcaaccgaac
     7741 ctacccgata gccacggaac agcaccggat ctcctggcga gagctgcccg gcttttttgc
     7801 tatcgagaat cacacggata cctttcgcat caggcggggc caacggtggt gagtccagca
     7861 aatcgtattt atccattttg ctgcctttcg cgcctggctg cagttcgata taaactccag
     7921 acagcagcgt tcccaggccg ctaatccctt cgcgaccaat ctgcggtttc accacccaaa
     7981 agacggtgtc tttatgcagc aatttttcca taccggaatt cagccgcgct ttgatttcaa
     8041 cgtgcgtcaa atcatcagcc agtgtggcgc tttcaaccac gccgacgtca acgctacggc
     8101 ttttaatggt ggttttgcca ccttcaattc cttccgcatt cgcggtgatc agggtcactt
     8161 ccggtccctg atggctgtaa tgataaaaaa gaacccaggc cccaatgagc gccgtgacga
     8221 taggaaatat ccacacggga gaccagttct tcactttctg gattttggct tccccattat
     8281 tagattccat actgtcagga ctcctcatgc tctgattcag gttgacgatc ccacgacaaa
     8341 cgcgggtcaa acgtcatagc agaaaacatt gtcattatga cgactaaagc aaacattaat
     8401 gcacccattg ccggataaat actcattaaa cctcccatac gcaccagcgc cgagagcacc
     8461 gcgataacga aaacgtcaat catcgaccag cggcctacaa actcaacaac ttcataaatc
     8521 aaatgcattc tttcactgtc gcgcttgcca tgccctttgg catcccaaca cagccacgcg
     8581 atggcgatca tctttaacgt tggcaccata atactggcca gaaagatcac cgcagcgacg
     8641 ggataagagc cttcgctcca taacagaatg accccagcga gaatcgtcga cggcatcttc
     8701 gagcctaata aatccgtcac catgatgggc aaaatattag ccggaagata cagcatgatg
     8761 gacgttacaa gcagcgcgag tgtccactgc aggctgtttc tacgccgaac gtaccctttg
     8821 gtactacaac gcgggcacac gggttcatca gcaggaagga ttgccgtaca acaggagcac
     8881 gaacgcagcc cctgacgtat ccccgtgacg cctggtttta gcggctggcg cagttctggc
     8941 atcggggcga tgtcgtccca taaccagcga cgatcaacgc actgaaaagc gcgcagttgc
     9001 aggacacaaa ataaacacca ggggagaaag ctgctgccta ccccaatgct gccgtaagcc
     9061 atcagtttaa cgaaactgac cagcacaccc gcgaggaaaa tctccgccat tccccaggtt
     9121 ttgagttgaa aaagcacccg tgccagttgc tcttttaaac ggaccggtaa ttccgcgcga
     9181 ttcaccagta acagaatggt tatcagacaa aacgcgggaa ccagttgcac aaacaacagg
     9241 aaaaaggtgc cgaggctggc gtagtcctca gaaaaaagca cgccgggaat ttccagtaat
     9301 gtaatttcac tggtaactcc tgcaacgttc atattcacaa aaggaaacaa gttggacagc
     9361 aacagcatga acagtgcagc caacgcatag gcggtgggac gctgccgagg ggcatcccac
     9421 gccacggtta acgttgtgcc acaccgggga catgccgctt tctgaccatg ctcaaggcgc
     9481 ggtaacgcca ccagcatgtc acactgcgag cacaggatgt gcttcgcggc atgatgatgt
     9541 tcgcacattg gtactcctta atgatgcgct attttacaga tgactaacaa agtgttggtt
     9601 ttcatgcctg ataaacccag ttcatcaggc aattttaccg ttgtcagctg tctctgttta
     9661 aatcgactat tttgcgatca gccaccattt tttaacgctt caagatactc ccagcgttca
     9721 aaggcttgct ccagctcctg ctctgcagca gccatatcag caagcacttt ttgcgtctgc
     9781 tcatgcggct gactgaagaa ggaagcatcc gccacttgcg tctgtagggc ttccagcttc
     9841 gcctccagat cttcgagcaa ttgcggtagc tgctccagtt cgcgctgcaa tttatagctt
     9901 agtttgctac tgctgcgttt tacagtttct gcttttggcg cggcggcttc ttcgcttttt
     9961 ttcaccgcag gctgtttgag tgccacatat tgttcttgct gaccacgggc atcatgataa
    10021 ccgccaacat aacgaccaat tttaccgccg ccttcgaaga tccagcattc tgtaacggtg
    10081 ttatcgacaa actgacgatc gtggctaacc agcaataccg tgccctgata gctgtcgatc
    10141 agttcttcca gcagttccag cgtttcgaca tcaagatcgt tggtcggttc gtcaagaatc
    10201 aataagttgc ttggtttgag gaacaaacgc gccagcagca agcggttccg ctcaccgcca
    10261 gaaaacgcac gtactggcgt catcgcccgt ttcggatgga acagaaagtc ctgcaaatag
    10321 cccaatacgt ggcgtggctt gccattaacc atcacctctt gcttaccttc ggcaaggtta
    10381 tccatcaccg ttttatcggg atccagttcc gcgcggtgct gatcgaaata agccacttcc
    10441 agtttggtgc caacgtgaat acgcccgctg tccgcttgaa gctgaccgag catcagtttc
    10501 agcagcgtgg ttttgccgca cccattcgga ccaatcaggg caattttgtc gccacgtaga
    10561 acctgggcag aaaaatcttt caccagttgc ttaccgttaa cctggtagca aacgtcttcc
    10621 atttcgaaaa cgatcttacc ggagcggctg gcctcttcca cctgcatctt tgcggtaccc
    10681 atcacttcgc gacgttcacc acgttcgcga cgcatcgctt tcagggcgcg tacgcggcct
    10741 tcattacggg tacggcgtgc tttgatcccc tggcgaatcc acacttcttc ctgcgccagt
    10801 ttgagatcga actcggcatt ttgtagttct tccacgcgca gggcttcttc tttttcctgc
    10861 aggtactggt cgtaattccc tggataggtc accagcttgc cgcgatcgag atcaacaatg
    10921 cgcgtcgcca tattgcggat aaacgaacgg tcgtgggaga tgaaaataat cgtcccattg
    10981 aaggttttaa ggaacccctc cagccagtcg atggtttcga tatccagatg gttagtcggt
    11041 tcgtccagca gcaatacgcg cggattgctc accagcgcgc gtcctaatgc cgctttacgc
    11101 aaccagccgc cggaaagcga cgacagcgca acgttaggat ctaaccccag ttgcgccagc
    11161 acttcgttga tgcggttttc cagctgccac aggttgtggt gatccagctg ttcctgaacc
    11221 ttcgccagtt cgttgagatt tttctcgctc gggtcgttca tcaccaggcg cgaaatatcg
    11281 tgatagcgtt tcagatattc cgcttgttct tcaatgcctt cggcaacgaa atcataaacg
    11341 ctaccctcaa cgttacgcgg tggatcctgt tgcagacgag ctacaatcaa atcttgctca
    11401 taaataatgc gaccgtcatc cagcccttgt tcacggttga ggattttcat taacgtcgat
    11461 tttcctgcgc cgttgcggcc caccagacaa acacgttcgt tatcttcgat atgcagttct
    11521 gcgttatcga gaagcggcgc gtcgctgaac gacagccatg cgccatgcat actgattaat
    11581 gacattacta ttcctttcag gctgcggtaa tcagccagca gttgtggatc tggcggttac
    11641 gggcgaaatc ctgggagagc gttttttgcg taatttcttg tgctttcagt cccagttttg
    11701 ccaggccgtc gagatccata cggaagccac gtttgttgtt cgagaacatg atcgtcccac
    11761 ctgcacgcag cagacgtttc aaatctttca tcagcgccag atgatcgcgc tgaacatcaa
    11821 acgcatcttc cattcgtttt gagttagaga aggttggcgg atcgataaag atcagatcga
    11881 actgttcatt tgcctcacgc agccacgcca ggcaatcggc ctgaatcagg cgatgcgcac
    11941 gcctagtcag gccattcaga cgcaggttgc gttctgccca ttccagataa gtacgcgaca
    12001 tatccacggt ggtggtgctg cgtgcaccgc ctaatcccgc atgcacggtg gcgctgccgg
    12061 tataagaaaa caggttgagg aaatctttgc ctttgctcat ctgaccgagc atacggcggg
    12121 cgatacggtg atcgaggaac agaccagtat cgagataatc cgtcaggttc acccacaagt
    12181 gagcgttata ttcggtaact tcaagaaact cgcccttctc gcccagtttc tggtactggt
    12241 ttttgccctt atggcgttca cgggttttca gcaccagttt gtttggcgca atccccagta
    12301 ccgaaatggt tgcagcgata atatcgaaca gacgctgacg cgctttgtgc gcatcaatag
    12361 tttttggcgg cgcatactcc tgcaccacca cccagtcggc ataacggtca acggcaacgt
    12421 tatattccgg cagatcggcg tcatacaggc ggtaacattc aatcccttcc tggcgtgccc
    12481 acttctcgaa ttttttgagg ttcttacgca ggcggttggc gtaatcttcc gctaccattg
    12541 ccggtttact gtctggtgtg ctttcggcaa cgtggtaatt tttctgtacg cagtccagcg
    12601 ggccgttttt cgccttgtac tgtttgtctg cacgcagctg caggcagctt agcagatccg
    12661 gcgaggcact aaacaaagag agattccagc caccgaactg gtttttcatg attcggccca
    12721 gcagactatg cagcgcaatc agcgccggtt cgctgtccag acgttcaccg tatggcgggt
    12781 tgctcaacac tgtaccgtac ggccctttcg gcagcggatt ggtcagttgc gcgacatctt
    12841 tcacctcaaa ggtgatcagt tcaccaatcc ccgcaagacg ggcgttagtg cgcgcacgtt
    12901 gaatcacccg tgcgtcgctg tccgaaccgt aaaaatgaga gctatactca gccaggcctt
    12961 tacgggcgcg agtttgcgct tccgctttca cttcctgcca gatagcttca tcatgctgcg
    13021 cccagccgct aaagccccaa cgcccacggt gcaagcctgg tgcgcgatcg gtcgccagca
    13081 tcgccgcttc aatcagcaac gtaccggaac cacacatcgg atcgagcagc ggtgttcctg
    13141 actgccagcc ggatcgcatc acaatagcgg ctgccagggt ttctttgatc ggcgcaatac
    13201 cagcacgatc gcgatagcca cgcagatgta aaccatcacc actcagatca agagcgatac
    13261 tggcggtttc tttatgcagc cagacgttaa cgcggatatc cggcgcatcg cgatcaacat
    13321 ttggacgcgg cagatttttc cgcgtgaaag catcgacgat cgcgtctttc actttcatcg
    13381 cgccgtactg actgttgcgg atggtgtcat tcaaaccact gaagtggaca gcgaaggtcg
    13441 cgccaggatt aaacatctct gtccagttga tcgcctgaac accgagatag aggtctaaat
    13501 cgctgtaaac cttacactcg cccagcggca acataatacg cgaggccagg cggctccaca
    13561 tcaggctctg gtaaacaagc cgtgtgtcgc ccttgaaatg gaccccaccc tgaaccacct
    13621 ggcattcaac ggcccccaga ttttccagtt cagtttttaa cagctcttcc agcccacggg
    13681 ccgtactggc aaacagagaa ttcatatcgt cacttttact ctaagagcct accgcagtag
    13741 gcgtaattgt tgcagtcagt ctggacatgg agagcgcgga gaaaccggag cgtacacaag
    13801 tacgtgagga tttcgagcac aacccagggc caaactgaca aataaaatag cctaacggga
    13861 caggcgctaa gaaaattgtt gcgcattata gctaataagc gcgtcatgtc ataaagttga
    13921 gggcttattt tcatttgagg accgcaccgt ggcgacatta acccggcttt ttattcatcc
    13981 tgttaaatcg atgcgcggca ttggtcttac acatgctctg gctgatgtca gtggtctggc
    14041 cttcgatcgc atctttatga tcacggaacc tgacggtacg tttattaccg ctcgccagtt
    14101 tccccagatg gtacggttta ctccttcacc tgtgcatgat ggcttgcatc tcaccgcacc
    14161 agatggcagt agcgcatatg ttcgttttgc tgatttcgcc acacaagacg caccaaccga
    14221 agtttggggc acacatttta ccgcgcgaat tgcgccagac gcgatcaaca aatggctaag
    14281 tggatttttc tcccgcgaag tgcaattacg ctgggtgggg ccacaaatga cccggcgcgt
    14341 gaaacgccac aacactgtac ctctgtcatt tgctgatggc tatccttacc ttcttgctaa
    14401 cgaagcctcg ttacgtgatc tccaacaacg ttgtccggcc agtgtaaaaa tggagcaatt
    14461 ccgcaccaat ctggtggttt ccggcgcgtc agcttgggaa gaagatagct ggaaagtgat
    14521 tcgcattggt gatgtggtgt ttgatgtggt taaaccttgt agccgctgta ttttcaccac
    14581 cgtcagccca gaaaaagggc aaaaacatcc ggcaggcgaa ccattaaaaa cattgcaatc
    14641 tttccgcact gcccaggata acggcgatgt cgattttggt cagaatttaa ttgcccgtaa
    14701 tagcggcgtg attcgcgttg gcgatgaggt ggaaattctg gcaacggctc cggcaaaaat
    14761 ttacggcgca gctgccgctg atgataccgc caacatcacg caacaaccgg acgcaaatgt
    14821 agatattgac tggcagggac aggcatttcg tggaaataac caacaagtgt tgctggagca
    14881 attagaaaat cagggaattc gtatccctta ttcttgccgc gcgggcattt gtggaagttg
    14941 ccgtgttcag cttttagaag gcgaagtcac gccgctgaaa aaatcagcaa tgggcgatga
    15001 tggcaccatt ctttgctgta gctgtgtacc gaagactgcc cttaagctga cgcgttagac
    15061 tgcctgttcg aggctgaagc tgtcaacatt cacctgcggt ttcagcctgt cgttcatgat
    15121 tttaatggcg tcgcccaact gcatcgcgcg acccgcaata acaacgcagg gttgtgccag
    15181 caggcatagc gcggcatttt cgccagattc aacgaccagc aaattgacct gctcgtgagt
    15241 atcgctcaac caaacgcagg ccgcatcgcc cggcatgggc acccacattt caccatggga
    15301 gacaaaatgc cagcttttcg gcatttgcgg tttgagataa cgaattgcta ccagtgcatt
    15361 cagcaccagt tcggcttttt ggtctttaga taaattaaaa tcgcggcact tttcttcaaa
    15421 agagaaatag agcgcggcat catcaacaca aaagcctgcg ggagaaaagg catctggagt
    15481 caacattttg cgcgcaaaac gtgagcgaaa tagcatacca ttggctaaat cgagcatcat
    15541 tcgatcgtgc tcttcatcgt aataccaacg ccagttatcg tctggtttaa ttcgcatctg
    15601 cctctcccct accctataaa accgcagcaa cacaatccat tgtcgcttcg cttaataacc
    15661 tcaacaataa aagtacaaaa tgtctaaata agcaacaacg atgaatataa aacaaccagg
    15721 gctgaaaata aagccctgat tatcttataa agaggaattc gaagaaataa ttagatatgg
    15781 gtaacgattt ctttaatcag cggcggacct ttaaaaataa aaccagaata aatttgcacc
    15841 agtgaggcac ccgcagcaat cttttcacgc gcagcgataa ccgagtcgat gccgccaaca
    15901 ccgatgatcg gtaagcgacc gtttaattct agtgacaagc ggcgaataat ttctgtgctt
    15961 tttaactgaa gcggacgacc acttaagcca ccggtttgat cgcaattttt cattccctga
    16021 accagagaac gatcgagagt ggtattggtt gcaataacgc catcaatatt atggcgaact
    16081 aaactatcgg caacctggat caattcgtct tcagaaagat ccggcgcgat cttcactgcg
    16141 atcggcacat atttatggtg catcgcttgc aaatcatttt gcttattttt aatcgcggtt
    16201 aagagatcat ccagcgcttc accatattgc agcgtgcgta atcctggggt attcggcgat
    16261 gaaatattga tggcgatata tcccgcatag gcatagattt tttccataca aatcagatag
    16321 tcatctttgc cctgctccac tggcgtatct ttatttttgc cgatattaat acccaagacg
    16381 ccgtcataat gggccttttt tacgttctct acgaggttat caacgccaag attattaaag
    16441 cccatacggt tgatcaaacc ttcggcatct accagacgaa agagacgcgg cttgtcatta
    16501 ccaggctgtg gacgtggcgt gacggtaccg atctcgatcg atccaaatcc catcgcgcct
    16561 aacgcatcaa tgcactcccc gtctttatca agaccggctg ccagaccaag cggattttta
    16621 aacgtcaggc ccatgcagtt aacaggtttc gcaggcactt tctgccgcac cagtgcttca
    16681 aacggtgttc cggtaatgcg gcgtaattgc tgaaaagtaa actcatgagc gcgctctgga
    16741 tcgagctgga aaagggcttt acgaacgaag gggtagtaca tgaactctcc tggattcccg
    16801 gtgtgcaaac cgggggcgta ttatgtgcga gttcggacca aaagggaatt gacctgcggc
    16861 aaaaaaaaag caaacgtttt cagaacctgt ttcagggcag attttgctcc ttctttgcac
    16921 tcaacccgcc gtaatcattt atcgtccgcc aggcgacatg acacggtgct attacctgac
    16981 accagtttgt ctgacgctgg cttttaggcg gcacgactcc cgcattactt aacgctttac
    17041 catttacgct cacctgaatc aggttaatgt agtaaggcgt tgggttagtg agttgtacac
    17101 cctgcgaatt ccgtgtccag cgtaattgtt gataagcttc cagcgggtcg cccggcaaac
    17161 cttcgggtcg ccagaacagt ttaaataccg agcgcatcgc cacttttacg ctctgatttt
    17221 caactttgcc ggatggcacg ctggcaatgc ttaactcaaa tagcgtttcg cgatccacag
    17281 gcaagatgtc gctttccgtt ctcagcaagc gcaacgtgcc tgtagtaccg ggcttcagga
    17341 gaataagtgg cggagcggcc agtaatagtg ctggcactgt cgacgcttcc cccccccggc
    17401 ccaacgcgtt gggcgattga ttttgctgtt aataagccaa gattcctgac tggtattagt
    17461 cagtaaaata gatatcgatt ctctgtctgc cggaaagata aatcgcgttc cgccgaccac
    17521 cactcctgcc tgcaattcac ccgccaccag cagcgataaa acggcgaaaa tcaagagcgc
    17581 taatctgttc cacgtattag tcataatgaa gtacaaatgt cgcgctggca ttcactttac
    17641 ccgactgcac atcgctattg acaggtcgca attgcgcata aaaagttaat ggcaccttac
    17701 cgctatgtag gctgtagtct ttactcgcct ggttaagcgg aatcaatatc ttcgcatcat
    17761 ccagaatcgc aattgccagc ccactggctg catccgtacc actttcaatg gacagcattt
    17821 cgctctgcac tccgtcgggg gttcctgaaa aggtgacatc aaccgtgctg acatctgttc
    17881 cgcaatcctg caaattaatg atgaaagatt tctccgggcc ataattccca tgaaaaaaac
    17941 gtgtcagcga gacatcgcct aactgcacat tgatttgctt gctatcctgt gccagcacgc
    18001 atgtattacg gcgaaacgat ccgctaatgt tgaccgtgcc atcatgggca aatagctcac
    18061 agccccccgt taatcccagt aagaacagaa tccagattat ccgtttcaac atcactcctt
    18121 cactggtaaa ccagatcaaa atagagaacc gccgtcgcat taccgcccgt tgctcccgcc
    18181 tttgtggact tataacgaag ttgatatttg agtgtgttat ccccggcttt taagggcgta
    18241 agaggctgga cctgattgag cgggatttct tgctgacttt gcgcatcaag aatttgcacg
    18301 gcaatccccg ttgccatacc accgcttccg cctgtgtcag ttagtttgag cagtgtcggc
    18361 gcaagggtgt cagcctcgcc gctaaaaagg acgtccgcgc cagtaatacc agcggcacaa
    18421 ccggtgatat caatattcaa atcagcagca gtgcttacgg tattcgcggc ctgaaaatca
    18481 ctgatattaa aataaccaat atgaatattt tgtaatgcac ttttggtggc gacatcacag
    18541 ccctgatcga ccacattccc ataaatatgc atactgactt gttcaggccc agccatcccg
    18601 acatggatgt taagaaggct aaagaacaaa ataacgcact gatatatcgt ttttttcttc
    18661 atacgttcac gcgttaatca aaatttattc tcaccgttgc cgtggcttca aatcgcccgg
    18721 tttctggtgg tacgcctgtc gtgctgactg gatagacatt cattcgcgga tactgtctgc
    18781 atcatcagga atagagagcg gtagtgtccc accagcaggg gaaataatgt tgttctggct
    18841 atcggtcacc accacgccca cgccaggacg tgacgtctta atcgcctggg gatagctggg
    18901 gtctgaggtt gccgtcagac tcagattgac gttaactaac cctgcgtttc ccgtgttaca
    18961 cctgaccgga attgcaagtt ctgccgttct ggccccaaga ggcttatgcc ctacaacacg
    19021 aaagttagcc gcaaatacgt cgcccagatc aacctcgatt gtttctccgg cattgacgga
    19081 acatgagcca agtgaattga tcgtaccaga caggtaatac actaaaaccg gtgtaccagt
    19141 agtcgtgcag gaatcacctg cgggtatgtt gtagcattca aataatcgcg ccagcgccat
    19201 aggaggaatg acgaccgaac caacgaacgg ctgcaatatt ttcagggaaa agctaccgct
    19261 attaccgcta gccccctggc cacctaaacg tagaccgccg ggatcgcaaa tatgccatcg
    19321 tgtatctgcc cctgtgccca cgttttcgaa aggaaccgca gtaggagaac tgcctttaac
    19381 ctgcaataaa actttggcgg caagatagtc attaattttg taccagttgt cgcctaaata
    19441 gactaacggt gaatccgcgg cgaatgccca tcggccactt ctgacattgt ctttatcgca
    19501 atcacaggaa acataataat cattcgattc gttccactga taaaatttgt ctttaactac
    19561 tccagccgta ttttcttctt catccagagt tttaacgcca aaattgacgc caatatacgg
    19621 attcccctga gtggtatgac actgcccgat ataagcagca gtttgagcgc accatagcat
    19681 taagacggcg gcaaaaaata gtcgtagtaa taacacgcat ttttctccaa agattatctg
    19741 catatggcat ccattcgcgt aatcgcagag ttaatttgtt gttcggtaag ctggtaactg
    19801 atatgacaaa tttgatcggg agcttcgccc cagcgtacag ttatcgctcc ctgagcgggc
    19861 aaaccggaca tatagagcga gccatcatca tcaataatgc cgctattagt ctgtatgccg
    19921 tccagcgtcg ctatcgcgcc aaaacgcagc ggtataccct gctttgatgt tttcattaat
    19981 acccttgctc ccgcatgggc attgaactcc gccagaacca acgccccttt ggtgggaacc
    20041 acgttgacta ctgcattttc aagatccacg ttacgtttta acgatgccgc atcaagtgcg
    20101 attcgatttt cccgataaga agtggcatag gggacaacgg tatatccgcg ccagtcagtt
    20161 ttcagcccgg tgctgttcgc cacacgagta ttctctgcgc ctggtgctgc aatcagcacg
    20221 ttagtttccc ccagcgattg ccccagggta atgccctgtg aatgggcaac taaactgcct
    20281 gaaagagcgt agttcagttg ttgttgactg ccgttatcgc tgtagttgta gcccactcgg
    20341 gcatctgcaa acgccccttt gtagtccatg ctggcgctac cattagccgt tttcccctcg
    20401 ctgttatatc cctgttgcac gctgtagctc aggttaccgt catcgagtag tgtctccgtt
    20461 aaacccaggt tttgtgtaat gtgcccttcg ttatcgatac tggtgttaga ggtcatccag
    20521 gcgtaatttt ttttcctggt atagctatca ttacttctgg ggagcaacag attgagcggc
    20581 agggaaaaat ttagtgcaaa cacctgatca gcatcaggtt gaccacggga cttactgtag
    20641 ttccaggaaa cccccagcga gagatcttta atttgcgtgt tgtagccaaa ctgtaatagc
    20701 cgatcctgtt gatcggtatg ccagtaagtt tgctggctac cacttaaata aaacaaaccg
    20761 tactcgccta attgctggga gatattgacc tgcagtttgc cacgtttggt gtaaaacaaa
    20821 ttgtaataac gcgaccacat cggcgtatct tcatcatcac cgtcattaaa ttcgtagcca
    20881 tccatatgtt tatacatggt gtcggaaagg gtgtagaaac ccgacgtcga atagcgatag
    20941 cccatcaatt gaaaattagt gcccaattgg ttgagcgttt tggcatacag aaaacgatat
    21001 gactgacctt tatgttcgct ttggtctgcc agcgtactct tcgcctgggt cgcatcgaac
    21061 gaaattgctc cgaaatcgcc aaggttaaat cccagaccaa acatggcggc acggtaatat
    21121 tcagcatatt gtccaccacc ataccatgtc gttccccacg gtccgcccca ctgcaacgtg
    21181 gtctgggcaa atttgctctc ctgctgttca ttactattgg ttctgtattt cgccagtgtc
    21241 accgcgtatt tgattcgccc ctgacgctgg agtaatggca cgctggaaaa cggtacgctg
    21301 tagctattga cgctgccgtc cgcttctttg atttcaacta acaaatcacc gctcgacgac
    21361 gtggaataga gatcactaat ttcaaaagca ccaggcgata cataagtttg gtaaatggtg
    21421 taaccatttt gcttaatcgt tatctgggca ttgcttttgg cgataccacg caccacaggc
    21481 gcaaaccctt tcaggctgtc tggcagcatg ttgtcatctg acgccagctg cacaccacga
    21541 aagctgacac tatcgaaaaa atcgcctgca gtataatcat cacctacggt cagttcgcct
    21601 tttagtggaa taaccgcccg ctgcaaatag ctgctgagat tcttccattc tgcggtttgg
    21661 ccactactgc gactccatgt tgaattgttg cgcaatctcc atgggcctaa attaacgcca
    21721 ctgtttaaat tcagaaaata gctgtcgcca gaatcactgt ctgcgctgct atgaatactg
    21781 ttagccccgc taaatagcgc gttgatccct tcatcccaac gttcaggggg gacataacca
    21841 cgggcattac ttttgatggc gatttgcggt actgaaattt ccagacggag tttattcaca
    21901 ttaaacgtta cgctggcatc cggaataatc tccgccaatg gcacacacac ttgtttgtcc
    21961 agttgtttaa aagccggaaa cgcatcagta ttaacaccca ttgcatccag gctttcagtc
    22021 gtcaaacagg cggcaatccc cttctctggc gttatctcaa caaaattgac gttgcgcgta
    22081 tctacaattg tctgattcag aa
//
LOCUS       DAJVRP010000066        21506 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVRP010000066 DAJVRP010000000
VERSION     DAJVRP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.066"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OQN12_002598"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OQN12_002598"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1654870.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OQN12_002599"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OQN12_002599"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1654871.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OQN12_002600"
     CDS             complement(3121..3420)
                     /locus_tag="OQN12_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1654872.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OQN12_002601"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OQN12_002601"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1654873.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OQN12_002602"
     CDS             complement(4632..5069)
                     /locus_tag="OQN12_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCS1654874.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OQN12_002603"
     CDS             complement(5115..5456)
                     /locus_tag="OQN12_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1654875.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OQN12_002604"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OQN12_002604"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1654876.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OQN12_002605"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OQN12_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OQN12_002606"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OQN12_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1654877.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OQN12_002607"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OQN12_002607"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCS1654878.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OQN12_002608"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OQN12_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCS1654879.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13864
                     /gene="xylR"
                     /locus_tag="OQN12_002609"
                     /pseudo
     CDS             12687..13864
                     /gene="xylR"
                     /locus_tag="OQN12_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13972..14796)
                     /locus_tag="OQN12_002610"
     CDS             complement(13972..14796)
                     /locus_tag="OQN12_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCS1654880.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="OQN12_002611"
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="OQN12_002611"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1654881.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="OQN12_002612"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="OQN12_002612"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCS1654882.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="OQN12_002613"
     CDS             complement(18728..19201)
                     /locus_tag="OQN12_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1654883.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OQN12_002614"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OQN12_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCS1654884.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="OQN12_002615"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="OQN12_002615"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCS1654885.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21506
                     /locus_tag="OQN12_002616"
     CDS             21362..>21506
                     /locus_tag="OQN12_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1654886.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5401 a         5488 c         5282 g         5335 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaactggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gtcttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgggcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcgcccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatata catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggcg cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acgcctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctgg ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag ggcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagcct gggctgaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgttgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgctcag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgcg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagttccgc atattgaaat tttaagccaa aaaagcgatc aaaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgcaaa
//
LOCUS       DAJVRP010000067        21387 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVRP010000067 DAJVRP010000000
VERSION     DAJVRP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.067"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..134)
                     /locus_tag="OQN12_002617"
     CDS             complement(<1..134)
                     /locus_tag="OQN12_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1654887.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="OQN12_002618"
     CDS             complement(236..1216)
                     /locus_tag="OQN12_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCS1654888.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="OQN12_002619"
     CDS             complement(1224..1934)
                     /locus_tag="OQN12_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCS1654889.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="OQN12_002620"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="OQN12_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1654890.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="OQN12_002621"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="OQN12_002621"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1654891.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="OQN12_002622"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="OQN12_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1654892.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="OQN12_002623"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="OQN12_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1654893.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /locus_tag="OQN12_002624"
     CDS             8511..8621
                     /locus_tag="OQN12_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1654894.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="OQN12_002625"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="OQN12_002625"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1654895.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="OQN12_002626"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="OQN12_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1654896.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="OQN12_002627"
     CDS             11503..12135
                     /locus_tag="OQN12_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1654897.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="OQN12_002628"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="OQN12_002628"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1654898.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15667..16599
                     /locus_tag="OQN12_002629"
     CDS             15667..16599
                     /locus_tag="OQN12_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1654899.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="OQN12_002630"
     CDS             complement(16640..18070)
                     /locus_tag="OQN12_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1654900.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="OQN12_002631"
     CDS             complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="OQN12_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1654901.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="OQN12_002632"
     CDS             complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="OQN12_002632"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1654902.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19931..20200)
                     /locus_tag="OQN12_002633"
     CDS             complement(19931..20200)
                     /locus_tag="OQN12_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1654903.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20270..20854
                     /gene="mobA"
                     /locus_tag="OQN12_002634"
     CDS             20270..20854
                     /gene="mobA"
                     /locus_tag="OQN12_002634"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1654904.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20836..21363
                     /gene="mobB"
                     /locus_tag="OQN12_002635"
     CDS             20836..21363
                     /gene="mobB"
                     /locus_tag="OQN12_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1654905.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5233 a         5365 c         5143 g         5646 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg
    18121 aaataatgtc cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa
    18181 aaaagcccgt gaatattcac gggctttatg taatttacat tgaattattt ttcctcggac
    18241 agatatttca ccgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata
    18301 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc
    18361 acatcagctg cggctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac
    18421 gccgcgtcgt actcttcacc tttaatacct gcattgataa atacatcgcg gatatcagat
    18481 gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct
    18541 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca
    18601 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca
    18661 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa
    18721 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca
    18781 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa
    18841 atctttttca tgatctactc tctccgatta atacataggt gttaattgca aagggggttc
    18901 ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc
    18961 ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caatgccgca ttagccaggc
    19021 aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc
    19081 gaactcgcta aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctgggc
    19141 tttatcgcca ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc
    19201 cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg
    19261 tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc
    19321 tgtcgctttc agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag
    19381 cttgcgaggc tcaatgagat attcattcag accgatggtc gggcgatgaa taaaaagctg
    19441 tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat
    19501 attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg
    19561 atgattcaat aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt
    19621 taccagctgc aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg
    19681 gcgataaaat ttgacgacaa aacgtcgacg ctcttcgtcc tgaaattgat agacacggtt
    19741 ttcatagctg ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc
    19801 gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc
    19861 atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc
    19921 ttacaagctt ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt
    19981 cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca
    20041 ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt
    20101 tcgccagttt ctgcaaaaat tgcaacaagt taaagtctgg ctctttttgc caggctggct
    20161 ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg
    20221 agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat
    20281 gacgacgata acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga
    20341 taaaggattg ctggacttaa atggcaaacc gctgtggcaa catgtcgctg acgcgcttaa
    20401 gacgcagctc tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg
    20461 cggtctgaaa gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct
    20521 ttcagtaatg cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta
    20581 cattccccat gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg
    20641 ggtccatgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc
    20701 tttattactg gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc
    20761 tggcggtcat gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc
    20821 agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg
    20881 agtggtaccg gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg
    20941 atccgtccgg ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa
    21001 gatagctatg agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga
    21061 tgggccttga tgacggaaac accagacgaa gaagagctgg atctacattt tctcgcaagt
    21121 cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc
    21181 gcaaagattg tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac
    21241 aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat
    21301 attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga
    21361 taagcaagag taggctggat cagacgc
//
LOCUS       DAJVRP010000068        21275 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVRP010000068 DAJVRP010000000
VERSION     DAJVRP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.068"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..444)
                     /locus_tag="OQN12_002636"
     CDS             complement(<1..444)
                     /locus_tag="OQN12_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS1654906.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OQN12_002637"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="OQN12_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1654907.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OQN12_002638"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="OQN12_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1654908.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OQN12_002639"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="OQN12_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1654909.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="OQN12_002640"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="OQN12_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1654910.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="OQN12_002641"
     CDS             complement(5214..6812)
                     /locus_tag="OQN12_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1654911.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OQN12_002642"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="OQN12_002642"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1654912.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="OQN12_002643"
     CDS             complement(8491..9069)
                     /locus_tag="OQN12_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1654913.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OQN12_002644"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="OQN12_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1654914.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="OQN12_002645"
     CDS             10508..10687
                     /locus_tag="OQN12_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1654915.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="OQN12_002646"
     CDS             complement(10807..11166)
                     /locus_tag="OQN12_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS1654916.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="OQN12_002647"
     CDS             complement(11528..11872)
                     /locus_tag="OQN12_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1654917.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="OQN12_002648"
     CDS             12004..13914
                     /locus_tag="OQN12_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1654918.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="OQN12_002649"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="OQN12_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1654919.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="OQN12_002650"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="OQN12_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1654920.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15493..17178
                     /gene="fadD"
                     /locus_tag="OQN12_002651"
     CDS             15493..17178
                     /gene="fadD"
                     /locus_tag="OQN12_002651"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1654921.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17260..18375
                     /gene="rnd"
                     /locus_tag="OQN12_002652"
     CDS             17260..18375
                     /gene="rnd"
                     /locus_tag="OQN12_002652"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1654922.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="OQN12_002653"
     CDS             complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="OQN12_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1654923.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19450..20067)
                     /gene="yeaW"
                     /locus_tag="OQN12_002654"
                     /pseudo
     CDS             complement(19450..20067)
                     /gene="yeaW"
                     /locus_tag="OQN12_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20409..20975)
                     /locus_tag="OQN12_002655"
     CDS             complement(20409..20975)
                     /locus_tag="OQN12_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1654924.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21187..>21275
                     /locus_tag="OQN12_002656"
     CDS             21187..>21275
                     /locus_tag="OQN12_002656"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1654925.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5268 a         5273 c         5466 g         5268 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta caacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcacc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat tgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatccttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcaggggttc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgggtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgacg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtcc cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaaaaag aaacagcggc tggtccgctg tttctgcatt cttacggtaa agataaaaat
    15361 aaatagtgac gcgcttcgca accttttcgt tgggtaatta tcaagctggt atgatgagtt
    15421 aatattatgt taacagcgtg tatatcattt ggggttgcga tgacgacgaa cacgcatttt
    15481 agaggtgaag aattgaagaa ggtttggctt aaccgttatc ccgcggacgt tccgacggag
    15541 atcaaccctg accgttatca atctctggta gatatgtttg agcagtcggt cgcgcgctac
    15601 gccgatcagc ctgcgtttgt gaatatgggg gaggtgatga ccttccgcaa gctggaagaa
    15661 cgcagtcgcg cgtttgccgc ttatttgcaa caagggttgg ggctgaagaa aggcgatcgc
    15721 gttgcgttga tgatgcctaa cttattgcaa tatccggtgg cgctatttgg cattttgcgt
    15781 gccgggataa tcgtcgtaaa cgttaacccg ttgtataccc cgcgtgagct tgagcatcag
    15841 cttaacgata gcggcgcatc ggcgattgtt atcgtgtcta actttgccca cgctctggaa
    15901 aaagtggttg ataaaaccgc cgttcagcac gtaattctga cccgtatggg cgatcagcta
    15961 tctacggcaa aaggcacggt agtcaatttc gttgttaaat acatcaagcg cttggtgccg
    16021 aaataccatc tgccagatgc catttcattt cgtagcgcac tgcataacgg ctaccggatg
    16081 cagtacgtca aacccgaact ggtgccggaa gatttagcct tcctgcaata caccggcggc
    16141 accacaggtg tggcgaaagg cgcgatgctg actcaccgca atatgctggc gaacctggaa
    16201 caggtcaacg ccacctatgg gccgttgctg catccaggta aagaactggt agtaacggca
    16261 ctgccgctgt atcacatttt tgccctgacc attaactgcc tgctgtttat cgaactgggt
    16321 gggcagaacc tgcttatcac taacccgcgt gatattccag ggttggtaaa agagttagcg
    16381 aaatatccgt ttaccgctat cactggcgtt aacaccttgt tcaatgcgtt gctgaacaat
    16441 aaagagttcc agcagctgga tttctccagt ctgcatcttt ccgcaggcgg tgggatgcct
    16501 gttcagcaag tggtggcaga acgctgggtg aaactgaccg gacagtatct gctggaaggt
    16561 tatggcctga ccgagtgcgc gccgctggtg agcgttaacc cgtatgatat tgattatcat
    16621 agcggtagca tcggtttgcc ggtgccatcg acggaagcca aactggtgga tgacgacgac
    16681 aacgaagtac caccgggtca accgggtgag ctttgtgtca aaggaccgca ggtgatgctg
    16741 ggttactggc agcgtccgga tgctacagat gagatcatca aaaatggctg gttacacacc
    16801 ggcgacatcg cggtgatgga tgaagaagga ttcctgcgca ttgtcgatcg taaaaaagac
    16861 atgattttgg tttccggttt taacgtctat cccaacgaga ttgaagatgt cgtcatgcag
    16921 catcctggcg tacaggaagt cgcggctgtt ggcgtacctt ccggctccag tggtgaagcg
    16981 gtgaaaatct tcgtagtgaa aaaagatcca tcgcttaccg aagagtcact ggtgactttt
    17041 tgccgccgtc agctcacggg atacaaagta ccgaagctgg tggagtttcg tgatgagtta
    17101 ccgaaatcta acgtcggaaa aattttgcga cgagaattac gtgacgaagc gcgcggcaaa
    17161 gtggacaata aagcctgagc gttaagtcag tcgtcagacg ccggttaatc cggcgttttt
    17221 tttgacgccc actaaagaga aaacaatttg aattaccaaa tgattaccac ggacgatgcg
    17281 ctggcttctt tgtgtgaagc cgtccgtgtc tttccggcga tagccctgga tactgaattt
    17341 gttcgtacgc gcacttatta cccgcagctg gggttgattc aacttttcga tggcgagcat
    17401 ctggcgctaa ttgatcctct cgggatcacc gactggtcac cgctgaaagc gatcctgcgc
    17461 gatccgtcca tcacaaaatt tctccatgca ggcagtgaag atctggaagt gttcctcaat
    17521 gtctttggcg aattaccaca acccttgatt gacacgcaaa tccttgctgc cttctgcgga
    17581 cgcccgatgt catggggttt cgcttccatg gtggaagagt attccggcgt tacgctggac
    17641 aagagtgaat cgcgcaccga ctggctggcc agaccgctga ccgaacgtca gtgtgaatac
    17701 gcagcggcgg atgtctggta tctgttaccg atcaccgcca agcttatggt agaaacggag
    17761 gcctccggct ggctacctgc ggcgctggat gaatgccgcc tgatgcaaat gcgtcgtcag
    17821 gaagtcgttg cgccggaaga tgcctggcgt gatatcacca atgcctggca gttacgcaca
    17881 cgccaactgg cctgtctgca actgttagcc gactggcgac tgcgcaaggc gcgagagcgt
    17941 gatctggcgg tgaactttgt cgtgcgtgaa gagcatttgt ggtcggtcgc gcgttatatg
    18001 ccgggaagtt taggcgaact ggacagcctg ggtttatccg gtagcgaaat ccgctttcac
    18061 ggtaaaacgc tgctggcgct ggtggaaaaa gcgcagacat tgccggaaga tgccttaccg
    18121 cagccgatgc ttaacctgat ggacatgcct ggttatcgta aagcgtttaa agcgattaag
    18181 tcgctgatta ctgacgtgag cgaaacgcat aagatcagcg ccgaattgct ggcatcgcgt
    18241 cggcaaatca accaactgct gaactggcac tggaaactga aaccgcagaa caatttgccg
    18301 gagctgattt ccggctggcg tggtgagctg atggcggaag cattacacaa tttattgcag
    18361 gaatatccgc agtaaaatct tccgaagccg gactgggcgc gctcagcgcc acatccggct
    18421 ttggcaaact acaaatccaa caccaggcgt ttacccttcg cacgcgaaca acagatcaac
    18481 atactttgct ggctggcacg ctcttcatcg ctaaaatact gatcccgatg gtcagcttca
    18541 ccttccagta ttgccgtttc gcaggttccg catacccctt cacgacataa acattccact
    18601 ttcgcggctt tattattttc aataacctgc aaaatagtca tctcttccgg caccacaaac
    18661 tcttttccgg aacgggcaag caccagggta aatgcatcgc cggttttgtc ttcgatagca
    18721 aattgctcaa agtgcagtgt atcggcggcg atgtccagac gcgcagcttc acttcttacc
    18781 gcttcaatta gcgcctcggg gccacaggta taaacgtgtg tgccaggttc gatatccgcc
    18841 aataatcgcg ctaattccag tcgtgttccg gttgatgaat gatgcaaatg gactttctca
    18901 gcctgcggat gctggactag ctcagcacga aatgcgcaac tttctggatt tcgcgagcag
    18961 taatgtagct gccagtcgat gtcgctgtgt tgcaactctg ccatgtgcga caggaaaggg
    19021 gggataccaa taccgcccgc gataaacaga tgctttctgg ctgagggaat tagcgcaaac
    19081 aggttattag gcgttgagat cgttaaccga tcgcccactt ttacctgctg atgcaaaaag
    19141 cgggaaccgc cgcgcgagtt ttcttccagt cgaacggcaa tctgataact agaggtgtta
    19201 tgcggtgaac tcagtagtga atacgcattg ctgtactggt tatcaccatc gctcatctgc
    19261 actaggatgt gacttcctcc ggtgaacgca ggcagcggtt tgccatcggt tgccaccagc
    19321 gtgaagcgtt tcacctgttc ggtaaggggt tcaacctggc tcacctgtac ttcaaacatt
    19381 tgatagtctg acattgctgt actccatcaa aaatcagccc agcggcggaa aataccgccg
    19441 ccgatgtcat tagtccttaa acacctgcgc cagcagatta tggaaatggg cgataccatg
    19501 ttcggaaatg ccactaccgc tactgtcggc catgatgcgc ccctgaccac gatagccacg
    19561 cgatttcagc cctttctgta cgctttcaac cagacgtaaa tcttccggat ggaacacatc
    19621 gcgataccac tcaatcagcg atttttgctc gtcggttaac tcttcattgg tgaagtaaat
    19681 atcgtagttt tgcagggtag tttcagaatc caccgggaat tcataaatga ccgtcatcat
    19741 ccctttgatc ggggtgacgt tcagcatcgt gcacggccac agccagaaac cgtggaatgc
    19801 cgcatccgta ccctcttcaa atttaaacga ctgttcggac ggtttggcga aaccgtattg
    19861 cagcgtccag ttaccgtgca tggtgtgcca gtaacgatca acctgtacgg agtcggagaa
    19921 acctagatgc gccggaccac agtgatagca ctcgagatag ttatcgacaa tgttcttcca
    19981 gttggcaggc gtgcgggtgg taaagcgggc cgctagtttc agattgtgga cttccgggca
    20041 ggcttccagc actttcgcgc ccagaccggt gtattgattc acttgaagta cgaaaaaaaa
    20101 ccgggaggac attggattat tcgggttctg atgggattag atttggtggg gcttgcaagc
    20161 ctgtagcgca aattttagtc gttaatcaat gaaacgcgaa agatagtaaa aaattgcttt
    20221 tgtttcattg aaaatacgaa aaacaaaaac actgcaaatc atttcaataa cagcttcaaa
    20281 aaacgttcaa aaccgataac aaccaggctg taaccaaatg actcatatca caaatcagct
    20341 catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt ttagctggtt
    20401 catggttgtt atacggcttt ttttacatct tgtggttctt gtgaaggagc tacaagacct
    20461 tcctgttcat aaataccata ctttgacaaa atctttttac gagtatctgt tggtaatgat
    20521 tcaagcattt tggctgtttg taaggtttgc tgcgaaatac cttgcaaatc agcctggatg
    20581 gaatcatcgg aagaaaaaat ctcaggcata agagcttcaa atcctttctt tctgaacaac
    20641 tcaatcgcag aatgcagttc cttgtcctct aacaattcga caattgcgat cagtttagtt
    20701 ttcaatgtgc tgcggtcagg ttgagaattt ttattctctt gattattttt atgctcaacc
    20761 attggcatgc tctgactttg gataaaccac tctattggac gccctgttgc gtaggctatt
    20821 tgtgctaatc gatctaaaga cgggtatgtc ttgccagaca ggtagtttct gataacccca
    20881 tcagaaagtc cgcacttagc tgcaaacgcc cggcttgatt ctccattcat tgcctcttca
    20941 aggcgttcct tgaaacgcat tatttccggg ggcataaaaa tgcgctctgc atttttgctt
    21001 tctttttcac ctgtcatgcc caatctctct gttatataaa gaattttaca gaattctttg
    21061 atttgcactg gttaaaataa aaatgagcat agctcttgaa ttgagcattt ttctgcggta
    21121 tcgtttttcc cgatagcggt agccggtatc agttaccgaa aacgttaaga gagtaaggat
    21181 cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc agataaaggc tgctgttcgt
    21241 atgaagggga agacactatc tcaattatcc agaga
//
LOCUS       DAJVRP010000069        21257 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVRP010000069 DAJVRP010000000
VERSION     DAJVRP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.069"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..793
                     /locus_tag="OQN12_002657"
     CDS             <1..793
                     /locus_tag="OQN12_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCS1654926.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="OQN12_002658"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="OQN12_002658"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1654927.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="OQN12_002659"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="OQN12_002659"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1654928.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="OQN12_002660"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="OQN12_002660"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1654929.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="OQN12_002661"
     CDS             complement(4991..7225)
                     /locus_tag="OQN12_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS1654930.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="OQN12_002662"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="OQN12_002662"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1654931.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="OQN12_002663"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="OQN12_002663"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1654932.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="OQN12_002664"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="OQN12_002664"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1654933.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="OQN12_002665"
     CDS             complement(12980..14320)
                     /locus_tag="OQN12_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1654934.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="OQN12_002666"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="OQN12_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1654935.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="OQN12_002667"
     CDS             complement(16108..16557)
                     /locus_tag="OQN12_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1654936.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="OQN12_002668"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="OQN12_002668"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1654937.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="OQN12_002669"
     CDS             complement(17357..17686)
                     /locus_tag="OQN12_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1654938.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="OQN12_002670"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="OQN12_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1654939.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="OQN12_002671"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="OQN12_002671"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1654940.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="OQN12_002672"
                     /gene_synonym="ygdH"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="OQN12_002672"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1654941.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5226 a         5672 c         5073 g         5286 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctggt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc atgccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatat
    16861 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctcccc
//
LOCUS       DAJVRP010000070        20837 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVRP010000070 DAJVRP010000000
VERSION     DAJVRP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.070"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OQN12_002673"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OQN12_002673"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1654942.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OQN12_002674"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OQN12_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1654943.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OQN12_002675"
     CDS             complement(2649..3200)
                     /locus_tag="OQN12_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1654944.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OQN12_002676"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OQN12_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1654945.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="OQN12_002677"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="OQN12_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS1654946.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OQN12_002678"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OQN12_002678"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1654947.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OQN12_002679"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OQN12_002679"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1654948.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OQN12_002680"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OQN12_002680"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1654949.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OQN12_002681"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OQN12_002681"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS1654950.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OQN12_002682"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OQN12_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1654951.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="OQN12_002683"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="OQN12_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1654952.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="OQN12_002684"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="OQN12_002684"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1654953.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENDWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OQN12_002685"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OQN12_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1654954.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OQN12_002686"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OQN12_002686"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1654955.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OQN12_002687"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OQN12_002687"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1654956.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OQN12_002688"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OQN12_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1654957.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="OQN12_002689"
     CDS             complement(18174..18569)
                     /locus_tag="OQN12_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1654958.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OQN12_002690"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OQN12_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1654959.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="OQN12_002691"
     CDS             19485..20078
                     /locus_tag="OQN12_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1654960.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="OQN12_002692"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="OQN12_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1654961.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4964 a         5226 c         5519 g         5128 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gatgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtaat tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tgactggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacatta ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg ggcaaaatat cagatttgaa
    20821 ttattctcaa cacatta
//
LOCUS       DAJVRP010000071        20825 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVRP010000071 DAJVRP010000000
VERSION     DAJVRP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.071"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..579
                     /locus_tag="OQN12_002693"
     CDS             <1..579
                     /locus_tag="OQN12_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCS1654962.1"
                     /translation="IKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVN
                     GRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQY
                     RMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVT
                     EYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            681..833
                     /gene="hokA"
                     /locus_tag="OQN12_002694"
     CDS             681..833
                     /gene="hokA"
                     /locus_tag="OQN12_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1654963.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1020..1232)
                     /gene="cspA"
                     /locus_tag="OQN12_002695"
     CDS             complement(1020..1232)
                     /gene="cspA"
                     /locus_tag="OQN12_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1654964.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1513..1803)
                     /locus_tag="OQN12_002696"
                     /pseudo
     CDS             complement(1513..1803)
                     /locus_tag="OQN12_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2237..2947
                     /locus_tag="OQN12_002697"
     CDS             2237..2947
                     /locus_tag="OQN12_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1654965.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2997..3971)
                     /gene="ghrB"
                     /locus_tag="OQN12_002698"
     CDS             complement(2997..3971)
                     /gene="ghrB"
                     /locus_tag="OQN12_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1654966.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4075..4725)
                     /locus_tag="OQN12_002699"
     CDS             complement(4075..4725)
                     /locus_tag="OQN12_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1654967.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4888..7221
                     /gene="bisC"
                     /locus_tag="OQN12_002700"
     CDS             4888..7221
                     /gene="bisC"
                     /locus_tag="OQN12_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS1654968.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7190..7630)
                     /locus_tag="OQN12_002701"
     CDS             complement(7190..7630)
                     /locus_tag="OQN12_002701"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1654969.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7627..8190)
                     /gene="tag"
                     /locus_tag="OQN12_002702"
     CDS             complement(7627..8190)
                     /gene="tag"
                     /locus_tag="OQN12_002702"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1654970.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8348..9046
                     /locus_tag="OQN12_002703"
     CDS             8348..9046
                     /locus_tag="OQN12_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1654971.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9275..10483
                     /locus_tag="OQN12_002704"
     CDS             9275..10483
                     /locus_tag="OQN12_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1654972.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10807..12498
                     /gene="eptB"
                     /locus_tag="OQN12_002705"
     CDS             10807..12498
                     /gene="eptB"
                     /locus_tag="OQN12_002705"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1654973.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12590..12666
                     /locus_tag="OQN12_002706"
     tRNA            12590..12666
                     /locus_tag="OQN12_002706"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12624..12626,aa:Pro,seq:cgg)
     gene            13410..15017
                     /gene="dppA"
                     /locus_tag="OQN12_002707"
     CDS             13410..15017
                     /gene="dppA"
                     /locus_tag="OQN12_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCS1654974.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15325..16344
                     /gene="dppB"
                     /locus_tag="OQN12_002708"
     CDS             15325..16344
                     /gene="dppB"
                     /locus_tag="OQN12_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1654975.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16354..17256
                     /gene="dppC"
                     /locus_tag="OQN12_002709"
     CDS             16354..17256
                     /gene="dppC"
                     /locus_tag="OQN12_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1654976.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17267..18250
                     /gene="dppD"
                     /locus_tag="OQN12_002710"
     CDS             17267..18250
                     /gene="dppD"
                     /locus_tag="OQN12_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1654977.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18247..19251
                     /gene="dppF"
                     /locus_tag="OQN12_002711"
     CDS             18247..19251
                     /gene="dppF"
                     /locus_tag="OQN12_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1654978.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19281..20552)
                     /locus_tag="OQN12_002712"
     CDS             complement(19281..20552)
                     /locus_tag="OQN12_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1654979.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5034 a         5119 c         5444 g         5228 t
ORIGIN      
        1 attaaggtca agcgataccg ctcttacaga ggagaggtag ggcaaaccgc ccctaatgtt
       61 ctccaaagag atttcaaggc tacgcggcca aacgagaagt gggttaccga tgttactgaa
      121 tttgcagtca atgggcgcaa gctgtatttg tctccagtaa tagatctctt caacaacgaa
      181 gttatttctt acagcctttc ggaaagacca gtgatgaaca tggttgagaa tatgctcgat
      241 caggcattca aaaagcttaa tcctcacgag catcctgttc tgcactctga ccagggatgg
      301 cagtatcgta tgagaagata tcaaaatatc cttaaagaac atggtattaa acaaagcatg
      361 tccagaaaag gcaattgtct ggataatgct gtggtggagt gtttctttgg aaccttaaag
      421 tcggagtgtt tttatcttga tgagttcagt aatataagcg aactgaagga tgctgttacg
      481 gaatatattg aatactataa cagcagaaga attagcctga aattaaaagg tctgactcca
      541 attgaatatc ggaatcagac ctatatgcct cgtgtttaac tgtccaactt tttggggtca
      601 gtacaaagtg aggctaccct atgcctgaac accataaggt tagcctctta ctcgttggaa
      661 atcaacacag ggggctggga atgccgcaga aatatagatt actttcttta atagtgattt
      721 gtttcacgct tttatttttc acctggatga taagagattc actgtgtgaa ttgcatatta
      781 aacaggagag ttatgagctg gcggcgtttt tagcctacaa attgaaagag taagagtctt
      841 cggcgggaaa ttattcccgc cttacttacg gcgttgcgca ttctcattgc acccatattt
      901 attcttcaca aaaataataa tagattttat tacgcgatcg attatttatt tcctgaaaaa
      961 aaataaaaaa atccccgcca aatggcaggg atcttagatt ctgtgctttt aagcagagat
     1021 tacaggctgg ttacgttacc agctgccggg cctttagcgc cgctttcgat ggtgaaggac
     1081 actttctgac cttcgtccag agatttgtaa ccatcgttct ggatagcaga gaagtgtacg
     1141 aacacatctt tagagccatc gtcaggagtg atgaagccga agcctttgtc agcgttgaac
     1201 cattttacga taccagtcat tttaccggac atagtgtatt acctttaata attaagtgtg
     1261 cctttcggcg atatggcgtg ctttacagat tttgaagcgt taaaggaatg tgcactacga
     1321 ggggtatcaa cgataactct tgaagggact tgccttacta cactgcttta atggtctgta
     1381 cgtcaaaccg ttgatgtgca ttaagccact cattggcggg tgatgcaaca attatttttc
     1441 atatttatga ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa cagcaataaa
     1501 gtggataaaa gtctatttca tcaactgctt acttaatgcc gggttggctt gaatcaaacg
     1561 catcaatttt agttcggcac ttgaaggctt cacgcgtctg gattcccatt cctttaccat
     1621 ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg tcccttttca
     1681 taactgctca atttcggtac aggacgttgt tctgtgcgtc agggtaattt tctgcgtttc
     1741 atctttaaaa acaatctgtt ccaggctgct caacagctca tgcattggat ctttatattc
     1801 cattgaaaac tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc tcattaagaa
     1861 tagtcgggaa aagcagacac agcggggtga agtcagggca tgctctggct atgattcgat
     1921 tcagcctgtc atttagcgtg acttatagcc acctaacgat ctgataaatg ccgatatatc
     1981 tcttatcact cctgttttgt aaagatttcg caaatctaac cttctgcgaa ggcgtaaatc
     2041 tctggctaca aaaaaggcaa cggcactatc attttttaac aatgaaacaa caaattaacc
     2101 ttgtttcatt ttctccatga ttttaccagg aagattacgt agaaagggta tcctgcaagc
     2161 ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc tttttttggg
     2221 tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg cgcctcttgc
     2281 ggcgttatta atggtagtta gcctgagtgg gtgttttgat aaagaaggcg atcagcgtaa
     2341 agcgtttatc gacttcctgc aaaatacagt gatgcgtagc ggtgaacgtc taccaaccct
     2401 gactgccgat cagaaaaaac agtttggtcc ttttgtctct gattacgcga ttctgtatgg
     2461 ttattctcag caggtgaatc aggcgatgga ttccggtctg cgcccggtcg tagacagcgt
     2521 taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc ggtccgctgc gtgaaatgaa
     2581 tggttcgctg ggtgtgctgg cgcaacaact gcaaaatgcg aagttgcagg ccgatgctgc
     2641 gcactctgcg ctaaaacaga gcgatgacct gaaaccggta tttgatcagg cattcaccaa
     2701 agtggtaacg acgccagctg atgcattgca accgttgatt ccggcggcac aaactttcac
     2761 gcaacaactg gtgatggttg gagactacat tgctcagcaa ggtactcagg tgagctttgt
     2821 cgcaaatggc atccagttcc cgacttcaca gcaggcaagt gaatataaca aactgattgc
     2881 gccattaccg gcacaacatc aggcgtttaa tcaggcctgg actacagcag ttactgcaac
     2941 ccaataaaga gtaaaagccc gagcgattat cgggctttac ttcacgcagt cgcagcttag
     3001 tccgcgacgt gaggattcac acagttcttc tcaacctttc cttgtaacgc atcaatcaaa
     3061 ttatccacgg cacaggcggc catgccataa cgcgtctcat gggtggcaga tccaatatgc
     3121 ggtactgcga cgacgttggc cattgagagc aacggcgaat ctacggacag tggctcttgt
     3181 tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct gcaatgctgc gatcagtgca
     3241 ttttcgtcaa ccaccgggcc acgtccggca ttaatgaaaa tggcggagga tttcattttg
     3301 gcgaattgtt ctgcgccaaa cagatgatgc gtctcatcag ttaacggcag gatcaggcaa
     3361 acgaaatctg actcttgtaa cagagtatcc aaatcgcagt agcgggcgtt gaagcgttct
     3421 tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa gccaaagtgc
     3481 acacgttgtg ccagcgccat gccgatccgt cccatcccga caatgcccag tgttttatgg
     3541 tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg tccattcgcc tgcttttacc
     3601 cgttctgcta cctccacaac ccgacgagcg gtagacaaca ccagcgccat cagcgtatcg
     3661 gcgacggttt ctgttaatac ggttggcgtg tgcatcagca gaatttttcg ggcggtaagc
     3721 gcatcgacat caaaattgtc atagccgacg gagatcgttg atgtggcacg cagtttcggc
     3781 attttttcca gcaatgcggc atctacattc tcgtttgaac ccagtaaacc ttcagcttct
     3841 gcaaaaattg ctgcattttg aacgacggtt tgtgggctga ggtttgccac ctggtgaacg
     3901 gtgaaatgcg cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta gaggataacg
     3961 gacggcttca tgcttctctc cattcactga taaggcgatc accttagcct gaaatatgat
     4021 gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa gggattacag
     4081 cgggcttaag gtaatttcta cacggcggtt ttgcgcctta ccttctgcgg tgctgttgct
     4141 ggcgattggg ttagccgggc caaggccctg agtacggatg cggctggcgt ccacgccctg
     4201 ggtgatcaac gcgctggcaa cggaatccgc acgctgctgg gagagacgca tgttcaggtc
     4261 gtgacccccc gtgctgtcgg tataaccaat cacgttaacc gccgttttcg gatactcttt
     4321 cagcaccatt gccacgccgg tcagggtgtt agcgcctgcc ggtttcaggg gagcgctgct
     4381 gctgtcgaag gtcacattgt tcggcatatt gaggataatg ttatccccgc tgcgggttac
     4441 gctaacacca gtgccgcgca ttttgtcacg cagcttcgct tcctgcacat ccatgtaata
     4501 acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca atcagcgcgc ctttaccgcg
     4561 atctttcttc gaagaagaga gcgcaccaat acccgcgccc acgagagagc ccagacctgc
     4621 gccgatagca gatttgcctg cttcgcgttc gccggtgtaa gggttagttg tgcagccaga
     4681 taccgccaga gcgccactca ctacggcggc aagtaagata aacacgtttc ttcattgtta
     4741 atccttaata acctttttat tctttgtcac gggttccgtg gcgggagatt atgccgcgtg
     4801 aacatgaaga ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat
     4861 acatctaacc tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg
     4921 actgccgccc actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg
     4981 cgtggcgcgt tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt
     5041 cacagcaata cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa
     5101 aacccgcaag gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg
     5161 gatcttattc accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt
     5221 gctggttcct acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa
     5281 cgctatatgg cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg
     5341 gcacaggcga tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt
     5401 tggccgctgg tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat
     5461 acgctgaaaa ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg
     5521 cgtgacagtg ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc
     5581 tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg
     5641 gggatcgccc atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc
     5701 accacaggtt atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa
     5761 aacgccgaat gggcggcaga gatttgtggt gttggcgcag cgaaaatccg cgagctggcg
     5821 gctattttcc accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag
     5881 tttggtgagc aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc
     5941 acacccggcg gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg
     6001 cgttctgcgg tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat
     6061 aaaatccctg ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac
     6121 aacggtatga accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac
     6181 tttactcatc atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg
     6241 gtgatctctg aatgcttctg gacggcggcg gcgaaacacg cggatattgt tctgcctgcg
     6301 actacctctt ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg
     6361 gtgccgatga agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt
     6421 gccgagttga gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt
     6481 cagctgcaat ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag
     6541 gttgaattgc cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa
     6601 aacccggaca gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat
     6661 ccgttaaaaa ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt
     6721 tacccggatt gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc
     6781 gaaccagaac agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg
     6841 aattacagtt ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat
     6901 cctgacgatg ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc
     6961 cgtgggcaga tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc
     7021 attcacgaag gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc
     7081 gcagtgaacg tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt
     7141 aatacgacgc tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt
     7201 gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat
     7261 cgacaatgtg aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa
     7321 cctccagcat cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac
     7381 cgcgtctgac ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc
     7441 tgacaaaacc gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat
     7501 aggcatcccg caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc
     7561 cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt
     7621 cccgaatcat ggtttatttc ccagatagca acagcagcca accacatgat cattcaccag
     7681 cccacatgcc tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt
     7741 tttcagtgcc ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt
     7801 tgtggcttgt gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cgggaaacgg
     7861 ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt
     7921 ccctcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat
     7981 tgctgcgacc ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt
     8041 gaggacggtg atccacgata acccagcctg ctgcccttca aagcagatca tttcgaacag
     8101 ttttttactg tcagtttcag gcacgcccca ctcattatca tggtaggcaa tataaagcgg
     8161 gccctgactc acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa
     8221 aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact
     8281 atgcccgata tccttcatac aatgacaata atattgccga attcggctct tccctggagt
     8341 cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt
     8401 ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga
     8461 tacgcaaccc ggacattcca cagagcgtta cacctgggat agtgatcatc aacctgatta
     8521 caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa
     8581 tctggcggaa gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc
     8641 tttatatgaa caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac
     8701 ctcgatgtat aacgagtttg gcgacagtac taccacgctg accgatccgt tatggcatgc
     8761 cagcgtgagt agcttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc
     8821 gcaaatcagc tataatcagc aatttggcga gaatatctgg aaggcgcaat caggcctgag
     8881 ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat
     8941 gttgctcaat caaaatattg ccgcctatgc tgcgctaact caggcagaaa ataccactaa
     9001 taatagcgac tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa
     9061 ttgaaaccat tgaataacaa cagttgtggc gatagtgggt gctaacttac caaataataa
     9121 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     9181 tggcatttca tgccgttttt ccccaagcat aaaatgcact tcgttatggt tgtcggcaga
     9241 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     9301 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     9361 ctggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc
     9421 tttctctttc ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt
     9481 gcaggaacgt tttggcgtta aacgcgtcac cgtggcttcc ggcattttgc tgggattagg
     9541 cttcttcctg accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct
     9601 ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt
     9661 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct
     9721 gggtttcaaa tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacctttgt
     9781 gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9841 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9901 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag
     9961 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
    10021 ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt
    10081 gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat
    10141 ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc
    10201 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt
    10261 cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt
    10321 cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac
    10381 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
    10441 gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta
    10501 ctgggcttca gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt
    10561 aaaatttggg ccagttaaca ggcccttttt actgcttttt gtaacgctaa agtctctttt
    10621 caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct
    10681 ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt
    10741 tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg tccagggttt
    10801 gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc
    10861 tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac
    10921 gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg
    10981 accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg
    11041 tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc
    11101 attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt
    11161 gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc
    11221 tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt
    11281 cgcagcctgg tggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg
    11341 gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt
    11401 gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg
    11461 gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca
    11521 taccaggctc cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg
    11581 cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag
    11641 gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg
    11701 cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa
    11761 cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag
    11821 agcgaaatgt ggttctacag caacacgatg gcagacaaca ttgcttatcg tgagcagatt
    11881 ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg
    11941 cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt
    12001 tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt
    12061 attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg
    12121 acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt
    12181 gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg
    12241 ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat
    12301 aaatatctgg aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac
    12361 atgaaagtgc cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat
    12421 acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa
    12481 gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg cattttgcta
    12541 ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc
    12601 gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac
    12661 cgaccaaatt cgaaaagcct gctcaacgag caggtttttt tgcatttgta gctcatgagg
    12721 atgagaacct ccgggggcag ggaggctcga ctcgacgtag gcctgataag acgcgccagc
    12781 gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg gcgcgaccgc cttattcgac
    12841 caacacacca cacattttcc tgccaacttt gcgccccata tctcactatc cattaacgga
    12901 tttgtgacag aaaccattgt ctttttttat atttgcttaa atcttttttg cgttacttat
    12961 ggctggcgct ggcattttcc gctgtgcgtt ttagcgttca tggtattaat cgctcagata
    13021 tccacctttt cctcagattt ttttcattaa atgcataaaa cttaatcacc aaatgttgct
    13081 aaatattaag ctgattgttc tggttatgtt ggagtagcac aaatttctct gctgcaaata
    13141 gctgtttgaa gtttttttaa tctttgtttg tggattctca ccgttggtgt aaatcccggt
    13201 tggctgtatt gacgttttca cattctgttg acagattgta ggtcacgagg ggcattttat
    13261 ggaggatccg cactgttaca ctgatgttaa ttagtacggc atccccacct cataacgttg
    13321 acccgaccgg gcaaaaaaca aaaaaggtca ggcagcgaca acccactgca aagggttaaa
    13381 acaacaaaca tcacaattgg agcagaataa tgcgtatttc cttgaaaaag tcagggatgc
    13441 tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc aagtgttcag gctaaaactc
    13501 tggtttattg ctcagaagga tctccggaag ggtttaaccc gcagctgttt acctccggca
    13561 ccacctatga cgcctcttcc gtcccgcttt ataaccgcct ggttgaattt aaaatcggca
    13621 ccaccgaagt gatcccgggc ctcgctgaaa agtgggaagt cagcgaagac ggtaaaacct
    13681 ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa taaagaattc aaaccgacgc
    13741 gcgaaatgaa cgccgatgac gtggtgttct cgttcgatcg tcagaaaaac gcgcaaaacc
    13801 cgtaccataa agtttctggc ggcagctacg aatacttcga aggtatgggc ttgccggagc
    13861 tgatcagcga agtgaaaaag gtggacgata acaccgttca gtttgtgctg actcgaccgg
    13921 aagcgccgtt cctcgctgac ctggcaatgg acttcgcctc tattctgtca aaagaatatg
    13981 ctgacgcgat gatgaaagcc ggtacaccgg aaaaactgga cctcaaccca atcggaaccg
    14041 gtccgttcca gttacagcag taccaaaaag attcccgtat ccgctacaaa gcgtttgatg
    14101 gctactgggg caccaaaccg cagatcgata cgctggtctt ctctattacc cctgatgctt
    14161 ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt gatgccgtac ccgaacccgg
    14221 cagacatcgc tcgcatgaag caggataaat ccatcaacct gatggaaatg ccggggctga
    14281 acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct ggatgacgtg aaagttcgcc
    14341 aagcactaac ctacgcggtg aacaaagacg cgatcatcaa agcggtttat cagggcgcgg
    14401 gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg ctataacgac gacgttcagg
    14461 attacaccta cgatcctgaa aaagcgaaag ccttgctgaa agaagcgggt ctggaaaaag
    14521 gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc gtataacccg aacgctcgcc
    14581 gcatggcgga gatgattcag gcagactggg cgaaagtggg cgtgcaggcc aaaatcgtca
    14641 cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg cgaacatcag acggtaatga
    14701 tgggctggac tggcgataac ggggacccgg ataacttctt cgccaccctg ttcagctgcg
    14761 ccgcctctga acaaggttct aactactcaa aatggtgcta caaaccgttt gaagatctga
    14821 ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt tgaactgtac aagcaagcgc
    14881 aggtggtgat gcacgatcag gctccggcac tgatcatcgc tcactccacc gtgtttgaac
    14941 cggtacgcaa agaagtcaaa ggctatgtgg ttgatccatt aggcaaacat cacttcgaaa
    15001 acgtctctat cgaataatta aaagccatac aagactgatg gcaaaggcaa aactgcctga
    15061 tgtgctccgc ttatcaggcc tacgaaaatt ctgcaatgta ttgaatttgc acgattttgt
    15121 aggccggata aggcgttaac gccgcatccg gcataaacaa agcgcacttt gtcaacaatc
    15181 tgtatacccg gtggcgctgt gcctctgcgt gcagcgccat ccggcagcaa tacttcattc
    15241 tctgtccgac aagggcggga attgatttgt gagcaataca gacacgcagt tccaggctgc
    15301 gagtcactat agagaatccg ggttatgttg cagtttattc tccgacgttt gggactcgtc
    15361 atccccacgt ttatcggtat tacccttctc acatttgcct ttgtccacat gatcccgggc
    15421 gatccggtga tgatcatggc aggcgaacgt gggatctccc ctgagcgtca cgtgcagctg
    15481 ctggctgaac tcggcttaga taaaccgatg tggcagcagt atctccatta catttggggc
    15541 gtaatgcacg gcgatctagg catttcaatg aaaagccgaa ttccagtatg ggaagagttc
    15601 gtgccgcgct ttcaggccac gctggaactt ggcgtctgcg cgatgatttt tgctacggca
    15661 gtcggtattc cggttggtgt gctggctgcg gttaaacgcg gttccatttt cgatcacact
    15721 gcagttggcc tggcgctgac cggctactcg atgccgatct tctggtgggg catgatgctg
    15781 atcatgctgg tttcggtgca ctggaacctg acgcccgtct ccggtcgcgt gagcgatatg
    15841 gtgttcctcg atgactccaa tccgttaacc ggttttatgc taatcgacac cgccatctgg
    15901 ggtgaagacg gcaactttat cgatgccgtc gcccatatga tcttgcccgc cattgtgctg
    15961 ggtactattc cgctagcggt cattgtgcgt atgacacgct cctcgatgct ggaagtgctg
    16021 ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa cccgcatgcg ggtgattatc
    16081 gtccatgcgc tgcgtaacgc gatgctgccg gtagtgaccg ttatcggcct ccaggtggga
    16141 acattgctgg cgggggcgat tctgaccgaa accatcttct cgtggcccgg tctggggcgc
    16201 tggttgattg acgcactgca acgccgcgac tatccggtag tgcagggcgg cgtattgctg
    16261 gtggcgacga tgattatcct cgtcaacttg ctggtcgatc tgctgtacgg cgtggtgaac
    16321 ccgcgtattc gtcataagaa gtaaggggac atcatgtcac aggttactga aaataaagtg
    16381 attagcgcac cggtgccgat gaccccgtta caggagttct ggcactattt taaacgcaac
    16441 aaaggcgcgg tcgtcgggct ggtttacgtc gccatcgtgc tgttcatcgc gatctttgcc
    16501 aactggattg cgccttataa cccggcggat cagttccgcg atgcgctgct cgccccgcca
    16561 gcctggcagg aaggcggcag tatggcacac ttgctgggca ccgatgacgt cggtcgtgat
    16621 gtgctgtcgc ggctgatgta cggcgcgcgt ttatcgctgc tggttggctg tctggtggtg
    16681 gtgttgtcgc tgattatggg cgttattctc ggcctgatcg ccggttactt cggcggcctg
    16741 gttgataaca ttatcatgcg cgtggtcgat atcatgctgg cgctgccaag cctgctgttg
    16801 gcactggtgc tggtggcgat tttcggcccg tcgattggta acgccgcgct ggcgctgacc
    16861 ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg ccgtgctggt ggaggtgaat
    16921 cgcgattacg tcaccgcctc tcgcgtggca ggtgccgggg cgatgcgcca gatgtttatt
    16981 aacatcttcc cgaactgcct tgcgccgctg attgttcagg cgtcgctcgg tttctctaac
    17041 gccattctcg atatggctgc tcttggtttc ctcggcatgg gggcacagcc gccaacgcct
    17101 gagtggggca ctatgctctc cgacgtgttg cagttcgcgc aaagcgcctg gtgggtcgtg
    17161 accttccctg gtctggcgat cctgttgacg gtgctggcat ttaacctgat gggtgacggt
    17221 ctgcgtgacg cgctcgatcc caaactaaag cagtaagagg ttcgagatgg cgttattaaa
    17281 tgtagataaa ttatcggtgc atttcggcga cgaaagcgca ccgttccgcg ccgtagaccg
    17341 catcagctac agcgtaaaac agggcgaagt ggtcgggatt gtgggtgagt ccggctccgg
    17401 taagtcggtc agttcactgg cgattatggg gctgattgat tatccgggcc gcgtaatggc
    17461 agaaaaactg gagtttaacg gccaggattt gcagcgtatc tcagaaaaag agcgccgcaa
    17521 cctggtgggt gccgaagtgg cgatgatctt ccaggacccg atgaccagcc ttaacccgtg
    17581 ctacaccgtg ggtttccaga ttatggaagc gattaaagtg catcagggcg gcaacaaaag
    17641 tacccgccgt cagcgggcga ttgacctgct gaatcaggtc ggtattcccg atccggcttc
    17701 gcgtctggat gtttacccgc atcagctttc cggcggcatg agccagcgcg tgatgatcgc
    17761 catggcgatt gcctgtcggc caaaactgct gattgccgat gaaccgacca ccgcgctgga
    17821 cgtgaccatt caggcgcaaa tcatcgaact actgctggag ctacagcaga aagagaacat
    17881 ggcgctggtg ttaattaccc atgacctggc gctggtggcg gaagctgcgc ataaaatcat
    17941 tgtgatgtat gcaggtcagg tagtagaaac gggcgatgca cacgccatct tccatgcgcc
    18001 gcgtcacccg tatactcagg cattgctgcg tgcgctgcca gaatttgctc aggacaaaga
    18061 acgtctggcg tcgttgccag gtgttgttcc cggcaagtat gaccgcccga acggctgcct
    18121 gcttaacccg cgctgcccct atgccactga cagatgtcgc gctgaagaac cggcgctgaa
    18181 tatgctcgct gatgggcgtc agtccaaatg ccattaccca cttgatgatg ccgggaggcc
    18241 cacactatga gtacgcaaga ggccacctcg caacaaccgc tgttgcaggc tatcgacctg
    18301 aaaaaacatt atccggtgaa gaaaggcatg ttcgtgccgg aacgtctggt gaaggcgctg
    18361 gacggcgttt cgtttaacct tgaacgtggc aaaacgctgg cagtagtggg cgaatctggc
    18421 tgcggtaaat cgaccctcgg tcggttgctg acgatgatcg aaacgcccac cggcggcgag
    18481 ctgtattatc aggggcagga tctgctcaag cacgatccgc aggcgcagaa gctgcgtcgg
    18541 cagaaaatcc agatcgtctt ccagaatccc tacggttcgc tgaatccgcg taagaaagtt
    18601 gggcaaattc tcgaagagcc gctgcttatt aacaccagct taagcaaaga acagcgtcgg
    18661 gaaaaagccc tgtcgatgat ggcaaaagtc ggcctgaaaa ccgagcacta tgaccgctat
    18721 ccgcacatgt tttccggcgg acaacgtcag cgtatcgcta ttgcccgtgg tctgatgctc
    18781 gacccggatg tggtgattgc cgatgagccg gtttctgcgc tggacgtctc agtgcgtgcg
    18841 caggtgctga atctgatgat ggatttgcag caggagttgg ggctttctta tgtctttatc
    18901 tcccacgacc tgtcggttgt ggagcacatt gctgatgaag tgatggtgat gtacctaggc
    18961 cgctgcgtgg agaaaggaac gaaggaccaa atcttcaata acccgcgcca tccgtacacc
    19021 caggcgctgc tctccgcgac gccacgcctg aacccggacg atcgtcgtga acgcatcaaa
    19081 ctcaccggtg aactgccaag cccgctgaat cctccgccgg gttgcgcctt caacgcccgc
    19141 tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc agctaaaaga ctacggaggt
    19201 caactggtag cttgttttgc tgttgatcag gatgaaaatc cgcagcgtta actcacaaaa
    19261 ccggggccat gcgctccggt ttactcaatc agcttcaaca gtggcgacaa gcatagcaaa
    19321 atgccgtaaa gcagaatcag ccaggcttta aatccgcgaa gtttttccag ttgtgcgact
    19381 ttatagatca ggaaaaacgg aatgaggcac gacacaatgc catataacgg gcttcccaac
    19441 tgaaagaaca ccagcactga tacacgaaac gaaacccaaa tcgtcaacgt aatgacgata
    19501 aaagcgcaga tcgccagagt cagcatgcgt gagttaattt tcttggtatc aataattcgg
    19561 cttaacagat tgagaataat gcctttaata gcctcgtgga aaccgagata aatgccaaag
    19621 aatgcggtca gtacggcaaa gatattcagc accgtagagg tgatatgaat gatatgtcca
    19681 gggatcacct gcgcggccag tgccagtgcc gagatatttt gttcaaaggc agaaacggct
    19741 tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa acaggatcac cgcgatgagc
    19801 gtgatataac taattcggtg ggtacgcagc gcgagccggg ttgccagtac tttatctgct
    19861 tcccgtttac gataggcaat attcattggg ttaagtacct gaataaatac cgcagaaaag
    19921 aagcaaaatg gaatggtaag caggacatcg cggaaaaaga cggaggcttg cgggaaggcg
    19981 gttatattgg cgaaattcca gtgcgggatc atcgcaaaac cgaacacgac aataatccct
    20041 actttgacca ccaccattgg cccagaaatc ttaaacagta atcgttcacc accagacgca
    20101 atcgctatca gtacggcgaa aatagcgact ttataaagta gagattgtga aagatcggca
    20161 tcggttaaac cgaaggtttt caggtacgag gcgctgtcga aaaccacgga gagagagtag
    20221 ataaaaatcc cgtggataat catcaaaaag tagataaccc cgaggaaaat tccccagttc
    20281 ttccccaggt aatgactgat aatatcggtg tagtcattac aggaatcgct ttcagaaagg
    20341 gtttttaaat aaatgtcctg cactacccag gtgacaggat aagcaatgat cgctgcggta
    20401 ataaataccc aaattccctt caagccaatt tgtactggca tcagcacggt tccggcacca
    20461 atcgccatgc ctatgcataa taaaacccag ccgaagtcgt agcgtgtaaa cggcaatttc
    20521 tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct gtccagaaag aagtcggcct
    20581 cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt ggagaagtcg aatacgcagt
    20641 atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg aggaggcgca attctacaaa
    20701 attgcgcatt tcactaccag tctggagtca gcccggatat gaactgcaac tattgcaaat
    20761 aaagaaatta agctggaagg catcgcgcaa tgaggaagat tgccgggtgc tgacagtaaa
    20821 aagca
//
LOCUS       DAJVRP010000072        20703 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVRP010000072 DAJVRP010000000
VERSION     DAJVRP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.072"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OQN12_002713"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="OQN12_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1654980.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OQN12_002714"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="OQN12_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1654981.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OQN12_002715"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="OQN12_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1654982.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OQN12_002716"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="OQN12_002716"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1654983.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="OQN12_002717"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="OQN12_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1654984.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="OQN12_002718"
     CDS             complement(8217..9110)
                     /locus_tag="OQN12_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1654985.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OQN12_002719"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="OQN12_002719"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1654986.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OQN12_002720"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="OQN12_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1654987.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="OQN12_002721"
     CDS             complement(10241..11473)
                     /locus_tag="OQN12_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1654988.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OQN12_002722"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="OQN12_002722"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1654989.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OQN12_002723"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="OQN12_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1654990.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="OQN12_002724"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="OQN12_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OQN12_002725"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="OQN12_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1654991.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OQN12_002726"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="OQN12_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1654992.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OQN12_002727"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="OQN12_002727"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1654993.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="OQN12_002728"
     CDS             complement(19832..20593)
                     /gene="ycaL"
                     /locus_tag="OQN12_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS1654994.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4980 a         5723 c         5029 g         4971 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac aacgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttctag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 agcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttagccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gcc
//
LOCUS       DAJVRP010000073        20697 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVRP010000073 DAJVRP010000000
VERSION     DAJVRP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.073"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            82..351
                     /locus_tag="OQN12_002729"
     CDS             82..351
                     /locus_tag="OQN12_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCS1654995.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="OQN12_002730"
     CDS             345..710
                     /locus_tag="OQN12_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1654996.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVVLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OQN12_002731"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OQN12_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1654997.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="OQN12_002732"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="OQN12_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1654998.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="OQN12_002733"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="OQN12_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1654999.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="OQN12_002734"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="OQN12_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1655000.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OQN12_002735"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OQN12_002735"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1655001.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OQN12_002736"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OQN12_002736"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1655002.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="OQN12_002737"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="OQN12_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OQN12_002738"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OQN12_002738"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS1655003.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OQN12_002739"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OQN12_002739"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS1655004.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14858..16456
                     /gene="rtcR"
                     /locus_tag="OQN12_002740"
     CDS             14858..16456
                     /gene="rtcR"
                     /locus_tag="OQN12_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCS1655005.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16438..17196)
                     /locus_tag="OQN12_002741"
     CDS             complement(16438..17196)
                     /locus_tag="OQN12_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1655006.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17213..18043)
                     /gene="glpG"
                     /locus_tag="OQN12_002742"
     CDS             complement(17213..18043)
                     /gene="glpG"
                     /locus_tag="OQN12_002742"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1655007.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18088..18414)
                     /gene="glpE"
                     /locus_tag="OQN12_002743"
     CDS             complement(18088..18414)
                     /gene="glpE"
                     /locus_tag="OQN12_002743"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1655008.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18604..20109
                     /gene="glpD"
                     /locus_tag="OQN12_002744"
     CDS             18604..20109
                     /gene="glpD"
                     /locus_tag="OQN12_002744"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1655009.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20378..20608)
                     /locus_tag="OQN12_002745"
                     /pseudo
     CDS             complement(20378..20608)
                     /locus_tag="OQN12_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4889 a         5692 c         5217 g         4899 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgttcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt tcagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcgcttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcaccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaattttt taatttattg
    14761 tttctaaaaa gattatatct ttatgtccgt aaacggagat ttcccgcaaa gccgatttac
    14821 cgataatgaa atatcgcctt ttataaggat atctaagatg cgtaaaacag tggcttttgg
    14881 ctttgtcggt accgtgctgg attatgccgg acgcggcagt cagcgctggt caaaatggcg
    14941 tccgtcactc tgtatatacc agcaagaatc gttggtcatc gatcgactgg aattattgca
    15001 cgatgcccgc tcgcgctcgc tatttgaaac gcttaaacgc gatatcgcca gcgtttcgcc
    15061 agaaacagaa gtggtgggcg ttgagattga actgcataac ccgtgggatt tcgaagaggt
    15121 ttacgcctgc ctgcatgatt tcgcccgtgg ttacgagttt cagccagaaa aagaagacta
    15181 tttaattcac atcaccaccg gcacccacgt cgcgcagatt tgctggtttc tgctggcgga
    15241 agcgcgttat ctacccgccc ggttgataca atcttcaact ccgcgcaaaa aagaacagcc
    15301 gcgcggccca ggtgaagtga cgattatcga tctcgattta agccgttata acgccatcgc
    15361 cagccgcttt gccgaggaac ggcagcaaac gctcgatttc ctcaaatccg gtattgccac
    15421 gcgtaacccc cacttcaacc gcatgattga gcagatcgaa aaagtggcga ttaaatcccg
    15481 cgcgccgatt ctgcttaacg gcccgaccgg cgcaggtaag tcatttctgg cgcgacgcat
    15541 cttcgagtta aaacaggcac ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc
    15601 caccctgcgc ggcgataccg cgatgtcgac gctgtttggt catgtgaaag gcgcgtttac
    15661 cggggcgcgg gaatctcgtg aaggattatt acgcagcgcc aacggcggaa tgttgtttct
    15721 tgatgagatt ggcgaactgg gcgcagacga acaggcaatg ctgctgaaag ctattgaaga
    15781 gaaaaccttt tacccgtttg gcagcgatcg ccaggtgagc agtgattttc agcttatcgc
    15841 cggaacggtg cgcgatttgc gccagctggt tgccgaaggc aaattccgcg aagatctgta
    15901 cgcgcggatc aatctctgga ccttcaccct gccgggtcta cgccagcgcc aggaagatat
    15961 tgaaccgaac ctggattatg aagtggagcg ccacgccaca ctcactggtg acagcgtgcg
    16021 ttttaacacc gaagcgcggc gtgcctggct ggcctttgca acatctcccc aggcaacatg
    16081 gcgcggtaac tttcgcgaac tttctgccag cgtcacgcgg atggccacct ttgccactag
    16141 cggacgcatc actctggacg tggtggaaga tgagataaac cgtctgcgct ataactggca
    16201 ggagagccgc ccctccgcgc ttacggcgtt gctgggcgca gaggcagaaa acatcgatct
    16261 cttcgaccgt atgcaactgg aacacgttat cgctatctgc cgccaggcaa agtcgctttc
    16321 tgctgccgga cgacagcttt ttgatgtttc gcgccagggc aaagccagcg tcaatgacgc
    16381 ggatcggcta cgcaaatacc tggcgcgttt tggtctgacg tgggaagccg tgcaggatca
    16441 gcacagctcc agttgaatat ggtggtccgt cagcacctgc atcacgctta ctggcggcgg
    16501 ggcgtcggtg tagacggcat caaccatgct gatgctcccc atattgacca tcgcattacg
    16561 gccaaatttt gaatggtcaa ccaccagcat aacgtggcgc gagttctcaa taatggcgcg
    16621 cttggtacga acttcgtggt aatcgaactc cagcagcgag ccgtcgctgt cgatgccgct
    16681 gatccccaga atgccgaaat caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc
    16741 aatgatcccg ccatcgcggc tacgtaattc gccaccggcg agaataatgc ggaaatcttc
    16801 ttttaccatc aacgtgttag caacgttgag attgttggtg acaatgcgca aattgctgtg
    16861 attgagcagc gcgtgcgcca ccgcttccgg cgtggtgccg atatcgataa acagcgtcga
    16921 gccattgggg atttgctccg ccactttgcg ggcgatgcgc tctttttctt cggtctgggt
    16981 ggctttacga tcgtgccacg gcgtgttaac cgaactggaa ggcagcgccg cgccgccatg
    17041 atggcgcagg atcaggtttt gctctgccag ctcgttgagg tcgcggcgaa tagtctgcgg
    17101 gctgacggag aagtgctcca ccagttcctc ggtactgaca taaccctgct gtttaaccag
    17161 ttcgataata ccgttgtgac gttgtgtttg tttcatttat aaatccctgg aattattttc
    17221 gttttcgcgc attgagcgaa tcaacaaaag ccatcgctaa acccacggct aatccggcga
    17281 tgtgtgctcc gttcgccatc gacatcccaa acaaatcaaa ccatccggcg acaatccaga
    17341 tcagcgcaaa gataattaac ccacgttgca ggtaaatgcc actttgcgga tcgcgttcgc
    17401 cacgtagcca gacgtagccc atcagcgcat acaccacgcc agaaagcccg ccaaaccacg
    17461 gcccgctgaa tttttgctgc atatagccgc ttaacagggc gctgataagc gtaatgacaa
    17521 ttagcttacc gctaccgagg cgtttttcca ccgcgccgcc gagataccac caccaaagca
    17581 ggttaaagag gatatgcatc agcgagaagt gcattaacgc gtgggtgaag taacgccaga
    17641 actcaaattt cagtgttgga tcgaatggcc aggccagcca taacatcact tcctgatcgc
    17701 cgagaatttg catggcaata aacaccacca cgcaggcgat catcatcacc caggtcaccg
    17761 gacctgcgcg ttcacgcaag gcagcaaaga aaggataacg gcgataatgc aggccactgc
    17821 cggtatggcc tgccagccag ctggccgcca gataacgcgg atctgccggg ttttcgagaa
    17881 aacgcgccag ctccgcccgc acgcgctcgg cctgggactc atccgccagc cagacatcgc
    17941 tttggttatg ttgttgaatc gtgaggataa caccctgcgt cgccatgtaa tcaacaaacg
    18001 cctgcgccac gcgggggtta gcaaaagagg taatcatcaa catcgttgct gtcgcttatt
    18061 ccacacaaaa ggggacagta taaagcgtta cgcgccgtac gccacctctg cgggaaactg
    18121 tcgctgccag gcttcaaagc cgccgtcaat gctatagacc acatcgtagc cctgttgcag
    18181 cagatactgc gccgcccctt tgctgctatt gccgtgataa cacatcacca tcaccggagt
    18241 gtcaaagtcg ttatcacgca taaacgcgcc cagcgtgtcg ttggttaaat ggaaagcctg
    18301 caccgcatgc cccatagcga aactctgtgg atcgcgaata tcgaccagca ccgcctcttt
    18361 ttcctgcaac ttctggtgcg cgtcggcaac gttaatacat tcgaactgat ccatgcgtct
    18421 ctctttcttt acaaacaagt gggcaaattt accgcacagt ttacgtcgaa gcggcagata
    18481 aacgccataa tgttatacat atcactctaa aatgtttttt caatgttacc caaagcgcga
    18541 ttctttgcta atatgttcga taacgaacat ttatgagctt taacgaaagt gaatgagggc
    18601 agcatggaaa ccaaagatct gattgtgata ggtggcggca tcaatggtgc tggtatcgcg
    18661 gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg aggcgcagga tctcgcttgc
    18721 gcgacctctt ccgccagttc aaaactcatt cacggtggcc tgcgctacct tgagcactat
    18781 gaattccgcc tggtcagcga ggcgctggct gaacgtgaag tgctgctgaa aatggctccg
    18841 catatcgcct tcccgatgcg ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg
    18901 atgattcgca ttggtctgtt tatgtacgat catctgggta aacgcaccag cctgccggga
    18961 tcaactggtt tgcgttttgg cgcaaattca gtgttaaaac cggaaattaa gcgcggattc
    19021 gaatattctg actgttgggt agacgacgcc cgtctggtac tcgccaacgc ccagatggtg
    19081 gtgcgtaaag gcggcgaagt gcttactcgg actcgcgcca cctctgctcg ccgcgaaaac
    19141 ggcctgtgga ttgtggaagc ggaagatatc gataccggca aaaaatatag ctggcaagcg
    19201 cgcggcctgg ttaacgccac cggcccgtgg gtgaaacagt tcttcgacga ggggatgcat
    19261 ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc atattgtggt gccgcgcgtg
    19321 catacccaaa agcaggccta cattctgcaa aacgaagata aacgcattgt gttcgtgatc
    19381 ccgtggatgg acgagttttc catcatcggc accaccgatg tggaatacaa aggtgatccg
    19441 aaagcagtga agattgaaga gagtgaaatc aattacctgc tgaaagtgta taacgcgcac
    19501 tttaaaaagc agctaagccg tgacgatatc gtctggacct actccggtgt gcgtccgctg
    19561 tgtgatgatg agtccgactc gccgcaagcc attacccgtg attacaccct tgatatccat
    19621 gatgaaaacg gcaaagcacc gctgctgtcg gtattcggcg gtaagctgac cacctaccga
    19681 aaactggcag aacatgcgct ggaaaaactg acgccgtatt atcagggtat tggcccggcg
    19741 tggacgaaag agagcgtgct gccgggtggt gccattgaag gcgaccgcga cgattacgcc
    19801 gctcgcctgc gccgccgtta cccgttcctg actgaatcgc tggcacgtca ctacacgcgc
    19861 acttacggca gcaacagcga gctgctgctc ggcaatgcgg gaacggtaag cgatctcggg
    19921 gaagatttcg gtcatgagtt ctacgaagcg gaactgaaat acctggtgga tcacgaatgg
    19981 gtccgccgca ccgacgatgc cctgtggcgt cgcacaaaac aaggcatgtg gctgaatgcg
    20041 gatcaacaat ctcgtgtgag tcagtggctg gtggaatata cgcagcagaa gttgtcgctg
    20101 gcgtcgtaaa ttaacgtaag gtgatcaggt cagatttcaa tctggcctga gactgatgac
    20161 aaacacaaaa ctgcctgatg cgctacgctt atcaggccta cgtggtttat gcaatatatt
    20221 gaatttgcat ggtcttgtag gccagataag acgttcacgt cgcatccggc atgaacaaag
    20281 cgcactttgt caaaaatcta acctactttt taattcaggg aattaccgca aagcccacgc
    20341 catcataccc aacgtaacaa aactcaggca cgttccccac tcgccccgag aaaaatagca
    20401 ttaatgcgcc cagcgtcagc ataaaaatat tgagcagaag gtaaattgtt ggcgtaaaga
    20461 gtaaattgca aacctcagga atagcggcaa gcccagcccc cagcatggca ataaccgctg
    20521 caaaaaattg cgtcagtatt gggatgccga aaaaagtcat taccagcgag gtcaaaatcc
    20581 attttctatt ttgcattatt ctttccattc tttctgaatg gcgaaattat actccccgag
    20641 tccccttgcc ccttctggac acttttccga aatgatggcg aaaaaaacgg gaccctt
//
LOCUS       DAJVRP010000074        20614 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVRP010000074 DAJVRP010000000
VERSION     DAJVRP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.074"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            98..397
                     /locus_tag="OQN12_002746"
     CDS             98..397
                     /locus_tag="OQN12_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1655010.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            407..931
                     /gene="ygaP"
                     /locus_tag="OQN12_002747"
     CDS             407..931
                     /gene="ygaP"
                     /locus_tag="OQN12_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1655011.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(978..1382)
                     /gene="stpA"
                     /locus_tag="OQN12_002748"
     CDS             complement(978..1382)
                     /gene="stpA"
                     /locus_tag="OQN12_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1655012.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2050..2499
                     /gene="alaE"
                     /locus_tag="OQN12_002749"
     CDS             2050..2499
                     /gene="alaE"
                     /locus_tag="OQN12_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1655013.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2536..2880)
                     /locus_tag="OQN12_002750"
     CDS             complement(2536..2880)
                     /locus_tag="OQN12_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS1655014.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3032..3262
                     /locus_tag="OQN12_002751"
     CDS             3032..3262
                     /locus_tag="OQN12_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655015.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3609..3854
                     /gene="nrdH"
                     /locus_tag="OQN12_002752"
     CDS             3609..3854
                     /gene="nrdH"
                     /locus_tag="OQN12_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1655016.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3851..4261
                     /gene="nrdI"
                     /locus_tag="OQN12_002753"
     CDS             3851..4261
                     /gene="nrdI"
                     /locus_tag="OQN12_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCS1655017.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4234..6378
                     /gene="nrdE"
                     /locus_tag="OQN12_002754"
     CDS             4234..6378
                     /gene="nrdE"
                     /locus_tag="OQN12_002754"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1655018.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6388..7347
                     /gene="nrdF"
                     /locus_tag="OQN12_002755"
     CDS             6388..7347
                     /gene="nrdF"
                     /locus_tag="OQN12_002755"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1655019.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7702..8904
                     /gene="proV"
                     /locus_tag="OQN12_002756"
     CDS             7702..8904
                     /gene="proV"
                     /locus_tag="OQN12_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1655020.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8897..9961
                     /gene="proW"
                     /locus_tag="OQN12_002757"
     CDS             8897..9961
                     /gene="proW"
                     /locus_tag="OQN12_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1655021.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10019..11011
                     /gene="proX"
                     /locus_tag="OQN12_002758"
     CDS             10019..11011
                     /gene="proX"
                     /locus_tag="OQN12_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1655022.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11203..12382
                     /locus_tag="OQN12_002759"
                     /pseudo
     CDS             11203..12382
                     /locus_tag="OQN12_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12506..13243
                     /gene="ygaZ"
                     /locus_tag="OQN12_002760"
     CDS             12506..13243
                     /gene="ygaZ"
                     /locus_tag="OQN12_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1655023.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13233..13568
                     /gene="ygaH"
                     /locus_tag="OQN12_002761"
     CDS             13233..13568
                     /gene="ygaH"
                     /locus_tag="OQN12_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1655024.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13659..14189
                     /gene="emrR"
                     /locus_tag="OQN12_002762"
     CDS             13659..14189
                     /gene="emrR"
                     /locus_tag="OQN12_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1655025.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14316..15488
                     /gene="emrA"
                     /locus_tag="OQN12_002763"
                     /pseudo
     CDS             14316..15488
                     /gene="emrA"
                     /locus_tag="OQN12_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15505..17043
                     /gene="emrB"
                     /locus_tag="OQN12_002764"
     CDS             15505..17043
                     /gene="emrB"
                     /locus_tag="OQN12_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1655026.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="OQN12_002765"
     CDS             complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="OQN12_002765"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1655027.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="OQN12_002766"
     CDS             complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="OQN12_002766"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1655028.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19401..19829)
                     /locus_tag="OQN12_002767"
     CDS             complement(19401..19829)
                     /locus_tag="OQN12_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1655029.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="OQN12_002768"
     CDS             complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="OQN12_002768"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS1655030.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4969 a         5276 c         5151 g         5218 t
ORIGIN      
        1 tcagcttacg cttttactct catttacgct gctgaggctg gtatatgttg catttttagt
       61 ttagagttta cttaatttag aaaatactta aataaatatg actgaactcg cgcaattaca
      121 ggccagtacc gaacaggcag cggccttatt gaaagcaatg agccacccta aacggttgct
      181 gattctgtgc atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac
      241 cggactgagc gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat
      301 caacagccaa cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc
      361 cattatcgcc accctgaaaa atgtctattg tccgtaagga gtcactatgg ctttgacaac
      421 catttcgccg catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg
      481 tgacgctgat gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt
      541 gctggaacag tcaggtcttc cacctaaatt acgtcgcgag caaattatat tccactgcca
      601 ggcaggtaaa cgcaccagta ataacgccga taaattagca gcgattgccg cccccgcaga
      661 aatattttta ctcgaagatg ggattgatgg ctggaaaaga gctggattgc cagtagcggt
      721 aaataaatct caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat
      781 attaatcggc gttgtactgg gttataccgt aaatagcggt ttcttcttat taagtggctt
      841 tgttggtgcc gggttactgt ttgcaggaat cagcggtttt tgtggaatgg caaggttgtt
      901 agataagatg ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc cctagcagcg
      961 acatccggcc tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc
     1021 tgagcaattg gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca
     1081 tcagtgaatt tatatttcgc cggacgcggc tggcgttttt taccagcacg tggtgcagca
     1141 gcagagctat tacccaataa ctcttccggg ttaattccgt cagctttcat cagctccagc
     1201 caggtgctaa ttttttcctg acgctcagcc agttcacgct gctgctgttc ttcttcttca
     1261 cgtctttctt tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag
     1321 aattcgcgag ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac
     1381 ataataaaga aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt agtgtaagcg
     1441 atcacttatt atatttccac aaaatttaat ctatttacag tcctgcgcga gattatttca
     1501 gctgttattt tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga
     1561 gtaatttagg ttattggtga acagagtggc gctctctgct cgctaactcc gcaagcctga
     1621 caaaaccgaa gtgaataaaa atgcaatata acagaataaa agaaaacacc cctcactggt
     1681 gctttctgct gttaatgtac attttctggc atcaaaaatg ctgtaaatga cccggtcacg
     1741 acaaacaccg aattctctac gcttatagcg ttacctcccc cccaaacttt cattagtgaa
     1801 attaaaggca agatcatatc taattgattt aattgaaaat aaatcatatc ggcatgtaaa
     1861 aatccacaat gtacaaaaaa cgagccgtta cggaatattt tatctacaaa aactaactaa
     1921 ataaaaattt ttcactaatt gattagtcat agacagcgat atacgctatg caaaaatgca
     1981 gatggcaatg agatccactg ctttcatctc cattaacatc ccattacgct tttattaagg
     2041 agcattagca tgttctcacc gcagtcacgc ttgcgtcatg cagttgcaga tacgttcgcg
     2101 atggttgttt actgttctgt cgtgaacatg tgtattgaag ttttcctctc cggaatgagc
     2161 ttcgaacagt ctttttattc cagattggta gcgattccgg tgaacatctt aattgcatgg
     2221 ccatacggta tgtaccgcga tctgtttatg cgcgcggcac gcaaagttag cccgtcgggc
     2281 tggataaaaa atctggctga tatcctggct tatgtgacgt tccagtcacc ggtgtatgtg
     2341 gcgatcttgt tagtggtggg cgcagactgg catcagatta tggcggcggt cagttcaaac
     2401 atcgttgttt cgatgttgat gggggcggtt tatggctact tcctcgatta ttgccgccga
     2461 ctgtttaaag tcagccgtta ccagcaggta aaagcctgac tcttcttttc gcgactggca
     2521 tcgccagtcg cgtctttaac tggcttcgcc aaataaacca ttcaaataac gttcaagcgc
     2581 aacacgcgaa ctaaagccat gcgggatacc gtaatgctcg tgtcgctcgc ctgctaaata
     2641 gagcggaaat tgctggtaat gatcgcaagt tttaatatcg gaagcgggtt cagccagtgt
     2701 tgaactgcgt tcttttaatg ttggatgaat taccagagcg gtacgtccca ttcgcgcttc
     2761 gcgattaaca tagacataat tttccccacg gcggtaacca tacgctttct gggttaccac
     2821 atcgacagta aaaccgactt tttcaagtac gcgcgccacc tcgtctggtc gtaaatacat
     2881 atttgatcct cgttattttc aaccgcgggc ctaccttacc tgattgcgca ttcacaacgc
     2941 tttcagaaaa gtccataaag ccgcgatgaa cctcagttaa gaaaattatg gtctacactg
     3001 aaaattacat cgaattctaa tgggggatca tatgtataac cgaccgaacc gcaacgacgt
     3061 tgatgatggc gtgcaggata tccagaatga tgtcaatcaa ttagctgaca gcctcgaatc
     3121 tgtattgaag tcctggggca gcgacgccaa aggggaagct gaagccgcac gcagtaaagc
     3181 tcaggcgttg ctgaaagaaa ccagggcgcg aatgcatggt cgtactcgcg tccagcaagc
     3241 cgcgcgcgat gccgttggct gagcggattc ttttgttcgt gaaagaccct ggtgtagcgt
     3301 gggtacagca gctgcggtag gtatttttat cggcgcactg ttaagcgtgc gcaaatcgta
     3361 gtgcaaaaat gataataaat acgcgtcttt gaccccgaag cctgtcttcg gggtttcttt
     3421 ttgcctggtg aatcacaaaa atccccctcc cccgtcacgc tcatatccag ggtaatttcg
     3481 atcactattt gctatatatt gtgtggttga atcttttttc aactacatct agtatctctg
     3541 tatcaacaga gagacaaccc gacgcgtatc atcgcgccgt atcttcattt taaacggaaa
     3601 tacgaatcat gcgcataact atttatactc gtaacgattg cgttcagtgc cacgccacca
     3661 aacgggcgat ggaaaaccgg ggctttgatt ttgaaatgat taatgtcgat cgcgttcctg
     3721 aagcagcgga agcgttgcgt gctcagggct ttcgtcagtt gccggtagtg attgctggcg
     3781 atcttagctg gtctggtttc cgtccggaca tgattaaccg tctgcatcca gcgccacacg
     3841 cggccagtgc atgagccagc tcgtctactt ctccagcagc tccgaaaaca cgcagcgttt
     3901 tatcgaacgt ttaggtctgc ccgcggtgcg catcccgctc aatgagcggg aacggattca
     3961 ggtagacgag ccttacatcc tgatcgtgcc ctcttacggc ggcggcggta cggctggcgc
     4021 ggtgccacga caggtaattc gctttttaaa cgacgagcac aaccgggcgt tgcttcgcgg
     4081 cgttattgct tctggtaatc gcaactttgg tgaggcgtat ggccgcgccg gagatgtgat
     4141 tgcccggaaa tgcggcgtgc cgtggctgta ccgttttgaa ctcatgggta cgcaaagcga
     4201 tatcgaaaac gttcgtaaag gagtaaccga attttggcaa cgacaaccgc agaatgcctg
     4261 acgcaggaaa cgatggatta ccacgcgctg aatgcgatgc ttaatcttta cgatagcgca
     4321 ggtcgcattc agttcgataa agaccgccag gccgttgacg cctttattgc gacgcatgtg
     4381 cgtccgaaca gtgtgacctt cagtagccag cagcagcgcc tgaactggct ggtcaacgaa
     4441 ggttactatg atgaaagcgt tcttaatcgc tactctcgcg actttgtcat tacgctgttt
     4501 gcccacgcac acaccagcgg ttttcgtttc cagacattcc tcggggcatg gaagttttac
     4561 accagctata cgttgaagac attcgacggt aaacgttatc tggaagattt tgccgatcga
     4621 gtcacgatgg tggcgctgac gctggcacaa ggcgatgaga cgctggcgtt gcaactgacc
     4681 gatgaaatgc tgtcaggacg ctttcagcca gccacgccaa cattcctcaa ctgcggtaag
     4741 cagcagcgcg gcgaactggt ttcctgtttt ttgctgcgta ttgaagacaa tatggagtcg
     4801 attggtcggg cggtaaattc cgcactgcag ctgtcgaaac gcggcggcgg cgtagcattt
     4861 ttgctgtcga atctgcggga agcgggcgcg ccaattaaac gtattgaaaa tcaatcttct
     4921 ggcgtaattc cggtgatgaa aatgttggaa gacgcatttt cctatgccaa ccaactcggc
     4981 gcgcgtcagg gggcaggtgc agtctattta catgctcatc atcccgatat tctgcgtttt
     5041 ctcgacacga aacgggaaaa tgccgacgaa aaaatccgca ttaaaacact gtcgctgggc
     5101 gtggtgatcc cggatatcac tttccatctg gcaaaagaga atgcgcagat ggcgctgttt
     5161 tcgccctatg acgttgagcg agtttatggc aagccgtttg ccgatatcgc cattagcgaa
     5221 cactatgacg aactggttgc cgatgaacgc atccgcaaaa aatacctcaa cgcccgtgat
     5281 ttcttccagc gactggcaga aatccagttt gagtccggct atccctacat catgtatgaa
     5341 gacacagtaa accgtgctag ccctatcgcc gggcgcataa atatgagtaa tctctgctca
     5401 gaaattttgc aggttaacag cgcctcagag tatgacgaga atctcgacta tgcccgcaca
     5461 ggccatgata tttcctgcaa tttaggttcg ttgaatattg cgcacaccat ggattccccc
     5521 gatttttccc gcacggtaga gactgccgtg cgcggtttaa cggcggtgtc agatatgagt
     5581 catatccgca gcgtgccgtc catcgaagcc ggaaatgccg cctcgcacgc catcggactg
     5641 gggcagatga atttacatgg ttatctggcg agggaaggca tcgcttatgg ttcgccggaa
     5701 gccctggatt tcaccaatct ctatttctat accatcacct ggcacgcact gcgtacctcg
     5761 atgttgctgg cgcgcgaacg cggtgaaacc ttcgccgggt tcaaacagtc acgctatgcc
     5821 agtggtgaat atttcagcca atatctgcaa ggtaactggc agccgaaaac ggcgaaagtt
     5881 ggcgaactgt ttgcccgcag ctgtattacg ttacctaccc gtgagatgtg ggcgcaactg
     5941 cgcgacgacg tgatgcgcta cggcatatac aaccagaatc tccaggcggt gccgccaacc
     6001 gggtctatct cttatatcaa ccatgctacg tcgagtattc atccgattgt ggcgaaagta
     6061 gagatacgca aagagggcaa aacaggacgc gtttactacc ctgccccgtt tatgactaac
     6121 gaaaatctgg cgctgtatca ggacgcttac gaaattggcg cagaaaagat catcgacacc
     6181 tacgcggaag cgactcgcca tgtcgatcag gggctgtcgc tgacgctgtt tttccccgat
     6241 accgccacca ctcgcgatat caacaaagcg cagatttacg cctggcgcaa aggtatcaaa
     6301 acgctctatt acatccgcct gcgtcagatg gcgctggaag gcactgaaat tgaaggctgc
     6361 gtctcctgtg cactttaagg aatatctatg aaactctcac gtatcagcgc catcaactgg
     6421 aacaagatat ctgacgataa agatctggag gtgtggaatc gcctgaccag caatttctgg
     6481 ctaccagaaa aggtgccgct gtcgaacgat attcccgcct ggcagacatt aactgtcgta
     6541 gaacaacaac tgacgatgcg cgtttttact ggcctgacgc tgctcgacac gctgcaaaat
     6601 gttatcggcg cgccttcgct gatgcccgat gcgctcacgc ctcatgaaga ggcggtatta
     6661 tcgaatatca gctttatgga agcggttcat gcccgctctt acagttcgat tttctcgacg
     6721 ctgtgccaga ccaaagatgt cgatgccgcc tacgcctgga gtgaagaaaa cgcaccgttg
     6781 cagcgaaaag ctcagattat tcagcaacat tatcgcggtg atgatccgct gaaaaagaaa
     6841 atcgccagtg tgtttcttga atcttttttg ttctattccg gtttctggct gccgatgtat
     6901 ttttccagcc gcggaaagct gaccaatacc gcggacctga tccgtctgat tatccgcgat
     6961 gaagcagtcc acggttacta cataggctat aaatatcaga aaaacatgga aaagatatct
     7021 ctgggacaac gtgaagagtt gaagagtttc gccttcgatt tgttgctgga actctacgac
     7081 aacgagttgc aatacaccga tgagctgtac gccgaaaccc cgtgggctga cgatgtgaaa
     7141 gcgtttctct gttacaacac caataaggct ttgatgaatc tgggctacga accgttattt
     7201 cccgcagaaa tggcggaagt gaatccggca atcctcgccg cgctttcgcc gaatgccgat
     7261 gaaaatcacg atttcttttc cggttcaggc tcctcttatg tgatggggaa agcggttgaa
     7321 acagaagatg aagactggaa tttctgaggg tgttattttc aaaaatatca ctacccgcag
     7381 cagggaaata attcccgcca aatagctttt tatcacgcaa atattctgta gtgatctaca
     7441 ctgatactct gttgcattat tcgcctgaaa ctacaatatt caggcgtttt ttcgctatct
     7501 atgacaaaaa atatcaactt ttctcgattt gctctcagcc cttatatcac ggaaaattcc
     7561 ggcgatttgc tcgcatcaat attcatgcca catttgccct caggggttgc ctcagattct
     7621 cagtatgtta gggtagaaaa aagtgactat tcccattggg taatatatcg acataaacaa
     7681 ataaaggaat cattctattg catggccatt aaattaaaaa ttaaaaatct ttataaaata
     7741 tttggtgagc atccacagcg agcgttcaaa tatatcgaac aaggactttc aaaagaacaa
     7801 attctggaaa aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa
     7861 ggcgagatat ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt
     7921 ctcaatcgcc tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc
     7981 aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag
     8041 tcctttgcct taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaattg
     8101 gccggaatta atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg
     8161 ctggaaaatt atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg
     8221 ggattagccc gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg
     8281 gcgctcgatc cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa
     8341 catcagcgca ccattgtctt tatttcccac gatcttgatg aagccatgcg tattggcgac
     8401 cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc
     8461 aataatccgg cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggtg
     8521 ttcagtgcga aagatattgc ccgccggaca ccgaatggct taattcgtaa aactcctggc
     8581 ttcggcccac gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtt
     8641 atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta
     8701 acgcagcagc aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa
     8761 acgcctctta gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc
     8821 gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat
     8881 cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga
     8941 cagtgccgcg caatccgcag acgcctgggg tacaccgacg actgcaccgg ctgacggcgg
     9001 tggtgctgac tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga
     9061 tccgttccat aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt
     9121 tgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa
     9181 cggtttccag caattgctgc tgggtatgcc agcgccagtg gcgattatcg ttttcgctct
     9241 catcacctgg cagatttccg gggtcggaat gggcgtggcg acgctggtct cgctgattgc
     9301 catcggcgca atcggtgcct ggtcgcaggc aatggtgact ctggcgctgg tgttaactgc
     9361 cctgctgttc tgtatcgtca tcggtttgcc gttggggata tggctggcga gaagtccgcg
     9421 agcggcgaaa attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta
     9481 tctggtgcca atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat
     9541 catctttgcg ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc
     9601 ggatctgatt gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt
     9661 tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc
     9721 cctttctatg gtggtcattg cctcgatgat tgccgtcggc gggctgggtc agatggtact
     9781 tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat
     9841 cctcgccatt atcctcgatc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg
     9901 caaccgtcgc tggtacacca ctggccctgt tggtctgctg acccgcccat tcattaagta
     9961 actctgcact tgcccggtgg cgccgggcat tatcaccctg ccaaaaaaag gaataacaat
    10021 gcgacatagc gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc
    10081 tgccgatctg ccgggcaaag gcattactgt taatccagtt cagagcacca tcactgaaga
    10141 aaccttccag acgctgctgg tcagtcgtgc gctggagaaa ttaggttata ccgtcaacaa
    10201 acccagcgaa gtggattaca acgttggcta cacctcgctt gcttccggcg atgcaacctt
    10261 caccgccgtg aactggacgc cactccatga caacatgtac gaagctgccg gtggcgataa
    10321 gaaattttat cgtgaagggg tatttgttaa cggcgcagca cagggttacc tgatcgataa
    10381 gaaaaccgcc gaccaataca aaatcaccaa catcgcacaa ctgaaagatc cgaagatcgc
    10441 caaactgttc gataccaacg gcgacggtaa agcggattta accggttgta accctggctg
    10501 gggctgcgaa ggtgcgatca accaccagct tgccgcgtat gaactgacca acaccgtgac
    10561 tcataatcag gggaactacg cagcgatgat ggccgacacc atcagtcgct acaaagaggg
    10621 caaaccggtg ttttattaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg
    10681 gaaagatgtg gtctggttgc aggtgccgtt ctccgcactg ccgggcgata aaaatgccga
    10741 taccaaactg ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc
    10801 caacaaagcc tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca ttatgcagtt
    10861 gccagtggcc gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg
    10921 cgatattcag ggccacgttg atggctggat caaagcccac cagcagcagt tcgatggctg
    10981 ggtgaatgag gcgctggcag cgcagaagta atttttattc gggcggataa ggcgttttcg
    11041 cctcatccgc cgctctgtac acaatgcctg atgcgacgct gacgcgtcct atcatgccta
    11101 caaaccacat cgcacatttc aacaagctat ctttcatccc atattcatca acatccgcta
    11161 ttattgattt ccagcttaat catcacctga tgaacaaaaa taatgactaa acctaatcat
    11221 gagcttagcc cggcgctgat cgtgctgatg tctatcgcca ccggtctggc ggtagccagt
    11281 aactattacg cccagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt
    11341 tcggcaggct ttattgttac cgccgcgcag ttgggctatg ccgcaggtct actgtttctt
    11401 gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggca
    11461 gcgggcggta tgttaatcac tgccagcagt cagtcactgg cgatgatgat cctcggtacg
    11521 gcattaaccg gtttattctc agtcgtggca caaattctgg ttccgctggc agcgacgctg
    11581 gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta tgagcggtct gctgttgggg
    11641 atattgctgg cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg gcgcaccgtc
    11701 ttttgggttg cttcggtgtt aatggcactg atggcgctgg cattatggcg tggtctgcca
    11761 caaatgaaat cagaaaccca cctcaactac ccacagttgt tgggttccgt tttcagcatg
    11821 ttcatcagcg ataagatcct gcgcacccgc gcgttgctgg gctgcatgac ctttgccaac
    11881 ttcagcattc tctggacctc aatggccttt ttgcttgccg ctccaccttt taactacagc
    11941 gatggtgaca ttggtctgtt tgggcttgcg ggagctgccg gagcgttggg cgctcgtccg
    12001 gcgggcggtt ttgccgacaa gggcaaatcg caccacaact ttcggtctgc ttctgctgtt
    12061 actttcatgg ctggcaatct ggtttggtca cacttccgta ctggcgttga ttatcggcat
    12121 tctggtgctg gacctcaccg tacagggcgt gcatatcact aaccagacgg taatttatcg
    12181 aatacatcct gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg
    12241 cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa catggcggtt gggctggcgt
    12301 ttgtctggct ggcgcgacga ttgccctggt taacttactg gtctggtggc gaggttttca
    12361 tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag
    12421 ggtgttaaga agcccatcag tctgataagg ttaagatatt cattcagtct atttataata
    12481 ttaacaatcg ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc
    12541 ggcgaccttc atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt
    12601 ggcctttgcg ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt
    12661 tttctcctgc atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc
    12721 cgggagtagt ttgtgggttg ctgcactgag cgtcatggca atggatgttc gccatgtttt
    12781 gtatggcccg tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct
    12841 atgggcgttt ggcctgacgg atgaggtttt tgccgccgcc accgcaaaac tggtacgcaa
    12901 taatcgccgc tggagcgaga actggatgat cggcattgcc ttcagttcat ggtcatcgtg
    12961 ggtatttggt acggtaatag gggcattctc cggcagcggc ttgctgcaag gttatcccgc
    13021 cgttgaagct gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc
    13081 tttccagcgc aaacaatctc tttgcgttac cgcagcttta gttggtgccc ttgcaggcgt
    13141 aacgctattt tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc
    13201 gttaatccag gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt
    13261 tactggttgg cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta
    13321 atgcccgccc aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct
    13381 cgatatgcgc tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt
    13441 tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca
    13501 ttatcatccc aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga
    13561 ttatataacc cacaagaatc atttttctaa aacaatacat ttactttatt tgtcactgtc
    13621 gttactatat cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca
    13681 ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc
    13741 ttctgactcg tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc
    13801 tgaaggctca ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg
    13861 aaaaccacag tattcagcct tctgaattaa gttgtgctct tggatcatcc cgtaccaacg
    13921 cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt aaaagcgata
    13981 acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg
    14041 ttttaccacc gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa
    14101 aagatcagct cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacaag
    14161 acggtgtggt tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat
    14221 aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc tttttggcaa gcaggtcggc
    14281 tcagccgatg agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa
    14341 ccccgcagca accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct
    14401 tgctctttat aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt
    14461 tcgaagaaac cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt
    14521 ctggcagcgt gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgatgttc
    14581 tggtcactct cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa actgcactgg
    14641 cttccagcgt tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata
    14701 ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc
    14761 tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca
    14821 gtgcccaggc gcaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc
    14881 tggggactaa actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg
    14941 cctagctggc gctggagcgt acccgtattg tcagtccgat gaccggttat gtttcccgtc
    15001 gtgccgtgca accgggtgcg caaattagcc caacgacgcc gctgatggcg gtcgttccag
    15061 ccaccaatat gtgggtggat gccaacttta aagagacgca gattgccaat atgcgcatcg
    15121 gtcagccggt caccatcacc accgatattt acggcgatga tgtgaaatac accggtaaag
    15181 tggttggtct ggatatgggc acaggtagcg cgttctcact gcttccggcg caaaatgcga
    15241 ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac
    15301 agctggagca atacccgctg cgcattggtt tgtccacgct ggtgagcgtc aataccacta
    15361 accgtgacgg tcaggtactg gcaaataaag tacgttcaac accggtcgcg gtaagtaccg
    15421 cgcgtgaaat cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg
    15481 ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg
    15541 gtcattatga cgattgcgct gtcactggcg acattcatgc aggtgctgaa ctccaccatt
    15601 gctaacgtgg cgatccccac tatcgccggg aatctgggct catcgctcag ccaaggaacg
    15661 tgggtaatca cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg
    15721 gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca ccatcgcctt tgctattgcg
    15781 tcttgggcgt gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag
    15841 gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa taactacccg
    15901 ccagccaaac gctcgatcgc gctggcgttg tggtcgatga cggtgattgt cgcgccaatt
    15961 tgcggcccga tcctcggcgg ttatatcagc gataattacc actggggctg gatattcttc
    16021 atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgt
    16081 gaaacccgca ccgaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt
    16141 atcggcagcc tgcagattat gctcgaccgc ggtaaagagc tggactgttt ttcatcacag
    16201 gaaattatca ttcttaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag
    16261 ctgaccgacg ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc
    16321 ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg acgctattgt tctgctgccg
    16381 cagttgttgc aggaggtcta cggttacacg gcgacctggg caggtttggc ctctgcgccg
    16441 gtagggatta ttccggtgat tctgtcgccg attatcggcc gcttcgcgca taaactggat
    16501 atgcggcggc tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc
    16561 tatacctttg aaccaggtat ggatttttgc gcgtcggcct ggccgcagtt tatccagggg
    16621 tttgcggtgg tctgcttctt tatgccgctg accaccatta cgctgtctgg tttgccaccg
    16681 gaacgactgg cggcggcatc gagcctctct aactttacgc gaacgctggc ggggtctatc
    16741 ggcacgtcga taaccacgac catgtggacc aaccgcgagt cgatgcacca tgcgcagttg
    16801 actgagtcgg taaacccgtt caacccgaat gcccaggcga tgtacagtca actggaaggg
    16861 cttgggatga cgcaacagca ggcatcaggc tggattgccc agcagatcac caatcagggg
    16921 ctgattattt ccgccaatga gatcttctgg atgtcagccg ggatattcct cgtactgctg
    16981 gggctggtgt ggtttgctaa accgccattt ggcgcaggtg gcggcggagg cggtgcgcac
    17041 taagtacaac taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt
    17101 tactgactag atgtgcagtt cctgcaactt ctctttcggc agtgccagtt cttcgttgct
    17161 gttgatgcgt acctcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga
    17221 gtgcatctgg taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg
    17281 cactttcagc acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg
    17341 cgtaccaatc agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat
    17401 ctctacacca ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt
    17461 atggatccct ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac
    17521 ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc
    17581 catccgggta tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat
    17641 ttttttaaaa attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg
    17701 catttgttat catcatccct gaattcagag atgaaatttt ggccactccc gagtggcctt
    17761 tttcttttct gtcaggcgtg tttttccagc cacaccgcaa acggttcggt atcagcggtt
    17821 tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc
    17881 agaatttcca gcggctcttc acgcagcaga ttacggtagg cttctgcaaa cgctttgcct
    17941 gttccgccaa taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg
    18001 ggattatcga agcaggcaac cagttcatca cacactttct gatacgcttc gccgccgtta
    18061 atgctatcca gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc
    18121 agtaacggga actgcgcggt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct
    18181 tcaaggatca cccggttcca gttaacgcgc gtacaggcaa gttcgctact gctcatttcc
    18241 ggtgcatcag ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca
    18301 tctacaccaa tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg
    18361 ccgccacgta acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg ttttggacga
    18421 atcggcgcat acagttcgtt ttcaatctgc agcacgttgc tattgatttg cagcctctta
    18481 ccgtctttct caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta
    18541 aggcccgcta cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta
    18601 ttggtatagc cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg
    18661 cactcggttt tctcaaacgg cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc
    18721 gccggagatg caccaaacag ataaggaatg acccaaccga aacgatagta attgcggata
    18781 acgcggaaat agcccgcaga aattttctct ttggcatcag cgcccgagat atcaccgcac
    18841 ttcgcttgcc agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc
    18901 atcagcgcgc cgtagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg
    18961 ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata
    19021 cttaacggcc acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc
    19081 ataaaggtca gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct
    19141 tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga
    19201 cctgttgttg ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc
    19261 tgtatcccct ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc
    19321 gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga
    19381 ccatatgtgc aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca
    19441 actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa agattactgg cccccaggag
    19501 atgcgcatcc atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat
    19561 aatagcgtga ctgcaccata gcgtttcagc cagccggtag ctttctcttg ccagcgcgat
    19621 gttttacgca atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta
    19681 cccattgttg ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc
    19741 accagcacga cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac
    19801 aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac atccacagcg tccatgcctg
    19861 ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt
    19921 gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga
    19981 cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg
    20041 cctcagcgat ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccaac
    20101 ttttcaccac ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg
    20161 cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa
    20221 ttgcctgagc aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt
    20281 actgaagacc gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctccgtat
    20341 ccaggattgt gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct
    20401 cgcaagtcga aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg
    20461 aaggagaaga aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa
    20521 ggaaattttt tggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
    20581 gtagccgagt actctatcca gctgagctac ggat
//
LOCUS       DAJVRP010000075        20569 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVRP010000075 DAJVRP010000000
VERSION     DAJVRP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.075"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            409..1239
                     /locus_tag="OQN12_002769"
     CDS             409..1239
                     /locus_tag="OQN12_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1655031.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OQN12_002770"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OQN12_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1655032.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OQN12_002771"
     CDS             complement(2368..3828)
                     /locus_tag="OQN12_002771"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1655033.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OQN12_002772"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OQN12_002772"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1655034.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OQN12_002773"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OQN12_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCS1655035.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OQN12_002774"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OQN12_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCS1655036.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OQN12_002775"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OQN12_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCS1655037.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OQN12_002776"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OQN12_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCS1655038.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OQN12_002777"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="OQN12_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCS1655039.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OQN12_002778"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="OQN12_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="OQN12_002779"
     CDS             complement(12470..13009)
                     /locus_tag="OQN12_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1655040.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OQN12_002780"
     CDS             complement(13074..13622)
                     /gene="fimA"
                     /locus_tag="OQN12_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1655041.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14102..14698)
                     /gene="fimE"
                     /locus_tag="OQN12_002781"
     CDS             complement(14102..14698)
                     /gene="fimE"
                     /locus_tag="OQN12_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCS1655042.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15176..15778)
                     /gene="fimB"
                     /locus_tag="OQN12_002782"
                     /pseudo
     CDS             complement(15176..15778)
                     /gene="fimB"
                     /locus_tag="OQN12_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            17232..17948
                     /gene="nanC"
                     /locus_tag="OQN12_002783"
     CDS             17232..17948
                     /gene="nanC"
                     /locus_tag="OQN12_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1655043.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17968..19074
                     /gene="nanM"
                     /locus_tag="OQN12_002784"
     CDS             17968..19074
                     /gene="nanM"
                     /locus_tag="OQN12_002784"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1655044.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19139..20119
                     /locus_tag="OQN12_002785"
     CDS             19139..20119
                     /locus_tag="OQN12_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1655045.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5478 a         4908 c         4672 g         5511 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 ttgtcgtttt ttaatatttt tatgcttgag aaaaaatacg taacttattt atgatatgga
    14041 cagtttggcc ccaattgtct tgtgtttatt tgttttttaa atttattatc aataagttaa
    14101 atcaaacctc ttctcttttt aatttttcgt ttatgagatt atttctttcc cataatccgg
    14161 caaaacgagc agcattactg gcggtataac gcacagtatg gcgaatattt cgattcccga
    14221 gataatcctg aattaaacgg gtatctgcac cacgctccgc cagttcataa ccgcaagcat
    14281 gccttaacat atgaggatga gtctgcgtta cggttccagc ttcaataccg gcatcgcgaa
    14341 taatgcgata ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata aatatagcgt
    14401 cggtccggtc agcgcctttc cagttagcac gttcctgggt ccagcgttcc acggcttcac
    14461 gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg cgaatattta
    14521 ttcgaccttc attaaggtca aggtcctgat aatgcagatc aagcagttca ctaatacgca
    14581 tcccatgccg atatgccaac agaataagac aataatctct ggctcccgtt gccccgtaac
    14641 aaaccgcctg catcatggcc tgaacttctt taccggtaag ataacgacgt ttactcacaa
    14701 tagtagtact cctaactgag atatatttga atgaatacct ataggaaacc tcaatcggtc
    14761 aaaattagcc caatagataa aaagataacc acacacgaag ttatgtggct atcagtcaat
    14821 tacattaaac atcatacaaa aataaaatat cactgatgtg acaacatcta ttttcattga
    14881 tttgattcaa tataacaata gattacagag gattaacttc cagttactta aaaaataaag
    14941 aaaaaagtgt ggaaacagga gaatttaagt gatgtgtaaa cttatgaatg attttgttta
    15001 tttattttga agaacataaa ccattagatt ttgtatgggt atggttaact taatgtttat
    15061 tatgataatt aagaatcaca cttacaataa catcaattaa taaatggtta atcatttttg
    15121 atatatcgta agaataatgt agtttttaac accatccctg gtatctcaac tatctctata
    15181 aaacagcatg acgctgtcgt cctctggctc tatcccagat gccgtaaaaa cgccctgcat
    15241 tgctggcggt ataccagaca gtatgacgaa tattgcgatg cccaagataa tcctagataa
    15301 gtcgcgtatc tattcccata ttcgccaaag caaaaccaca cgaatagcgt aacatgtgcg
    15361 gatgaatctc cagtgacaac ccggcattac caccagaagt cgagataata tggtaaaact
    15421 gttgccgaga aagcggattc cccttacgtg ataaaaatac ccactcgctc tcagcatgcg
    15481 gataagaagt acggatactc aaccagtttt ttaaagcctg aacttctttg ttcaatagcg
    15541 ggtgcgttgt tgaaaagcct ttttttaatc gatggatata tatacacttt gctttaagat
    15601 caatatccga aatcctcaat cgacaaattt cactcgcccg gaaaccatga ataaaacaaa
    15661 gcaaaatcag acaataatta cgtgttgcat gaggcccggt atttgctgct ttaaggagtg
    15721 actcgatttc actatgggta aggaagttcc tttttttgtt atcagcctta ttcctcatcg
    15781 ttttccctta taattacaga cgcgcactag ctgtgctggg ttagcaataa tccaacattt
    15841 ttatcctgat tatgtttata aaagcgaacg tttgcttaat aacttacgta agtggaccag
    15901 ttcttctgag cgaacttaaa tggagtagcg atagttactt ataataactt tgccatacaa
    15961 acgccatgct cttgcatgct atgtaccttt atatatttat caatctgata cgaatctaat
    16021 aataaccctc atcattaatg caactaatct tatctatata tcatgtgata tgttctgtaa
    16081 caagtaaaat caacataaaa aattcagcaa aatttaatat ttgttaaact agccgtctca
    16141 aatatgacat tattagttaa ctgcatttgc cacaaaatgg ctatttcatg gaacgcagta
    16201 atttcttagt ggctattatc atgctaattc ttagatccct agcgattatt cctgcctggt
    16261 tcgtcagctt aggccatgag attgttacat tcctttttat cctggagata tgggggaacg
    16321 caggcgacgc tttttcatct acatccgttg gtaaatatag ctttggtcac tacgacttaa
    16381 gccttcctca atcacagcta ttcgccgggc gatacggcaa acaaactaag tgaaacatca
    16441 tcctgaaaac accaacatca acaagcctct ccagatcgac atcaggagtg accagttaca
    16501 agccacaaac caagaaacat atttcatgat gagaattatg ctcagcctga tggcgagtaa
    16561 acccttattt tcaccactct cactgagtta gcttgcaaag ctttctttgt ttagattcta
    16621 aataaattat ttataaaaca tatagttaaa agaaaatctt tagtttttga agaccaccgc
    16681 aagtgttcgt ctggcttcac atggcatctt cttcttagaa aaagatcgac atattttgtg
    16741 acgcgaattg caaatctggt tttgttgtat ggattgcgtg atttttgatc tggtataaca
    16801 ggtataaaga tgcaccacga tagtcaatga gactgggcat ctcgtaatct atggcaaaca
    16861 tcacttcagt tctttcacat cgggtgatga aaatgcactt cagtctgaaa ggaatatgaa
    16921 aatgagatca acagacactc tattttatga ctctgggtaa aatgggttga gtaagtgata
    16981 taacttacga atattcaaat caattaaaaa tcagaagaga ttttatactc aggtatttaa
    17041 tctggatctc tgtttattta aataatgtta aaagagaatt ttcacaggag accttataca
    17101 aaaaatataa aatacagtta cccgttgcca aagacactat aagcctggca aaaaaatatt
    17161 acacaacata aatgctaatt gtttatgcgg gctttgtatt gctttctgta tcctacaaat
    17221 gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt attattactg tgcttttcgt
    17281 ccccattaat ttctcaggct gcgacactgg acgtacgtgg tggatatcgt agtggaagcc
    17341 acgcctatga gactcgactc aaagtcagtg agggatggca aaatggatgg tgggcaagca
    17401 tggaaagtaa tacctggaat accattcatg ataataaaaa ggaaaatgcc gcactcaatg
    17461 atgttcaggt tgaagttaat tacgcaatta aacttgatga tcaatggacg gtgcgcccgg
    17521 gaatgttaac gcattttagc agcaacggca cacgctacgg accctacgta aaactgtcct
    17581 gggacgcgac aaaagatctt aattttggca ttcgctatcg ttacgactgg aaagcttacc
    17641 gacaacaaga cttatccggt gatatgtctc gtgataacgt tcatcgttgg gatggatatg
    17701 tcacttacca tattaatagt gatttcacct tcgcatggca aacgacgcta tacagcaaac
    17761 agaatgatta tcgctatgca aaccacaaga aatgggcgac ggaaaatgcc tttgttctac
    17821 aataccatat gacgcccgat attacgccat acatagaata tgactacctt gaccgccagg
    17881 gtgtttacaa cggcagagat aatttatcgg aaaacagtta tcgcattggt gtgtcattta
    17941 aactgtagta gacaggagac agtcacaatg aataaaacta taatggcgct tgctatcatg
    18001 atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa ctcctgtgcc atttaaaagt
    18061 ggtaccggag caattgataa cgacactgtc tacattggtt taggtagcgc aggtacagca
    18121 tggtacaagc tagatacaca ggccaaagat aaaaaatgga ctgcgttagc tgcattccct
    18181 ggtggaccga gagagcaagc aacctctgca tttattgatg gcaatctgta tgtgtttggc
    18241 ggcattggca aaaacagcga gggattgact caggtattta atgacgtaca caaatacaac
    18301 cccaaaacca atagctgggt taaattgatg tcgcacgcgc cgatgggcat ggcgggtcat
    18361 gtgacttttg tacacaacgg caaggcttat gttactggcg gtgttaacca gaatatcttc
    18421 aatggctatt ttgaagatct caacgaggct ggaaaagatt caaccgctat agataaaatc
    18481 aacgcccact attttgacaa aaaagcagaa gattatttct tcaataagtt tctgttgtct
    18541 tttgatccct caacacagca atggagttac gctggcgaat cgccctggta cggaacggct
    18601 ggtgcggcgg ttgtgaataa aggtgataaa acctggctta ttaatggcga agccaaacca
    18661 ggattgcgaa cggatgccgt atttgaactt gatttcaccg gtaataattt aaaatggaat
    18721 aagcttgatc ccgtctcatc accagatggc gtcgctggcg gttttgcggg gataagcaat
    18781 gattctctta tatttgccgg aggggccgga ttcaaaggtt cacgagaaaa ttaccagaac
    18841 ggtaagaact atgcgcatga aggcctgaaa aaatcatata gcactgatat tcatctttgg
    18901 cataacggga aatgggataa atcgggtgaa ttatcgcaag gtcgggccta cggagtatca
    18961 ttgccctgga ataatagttt attaattatt ggcggtgaaa ctgcaggcgg caaagcggtg
    19021 acggattcag ttttaatttc tgtgaaggat aataaagtca cagtacaaaa ctaacgcttc
    19081 agggccccgg taaaggggcc ctgctatcaa tttgtcattt gtattacgcg aaaattatat
    19141 gaacgcaata atatcacccg attattacta tgttcttacc gttgctggtc agtctaatgc
    19201 catggcgtat ggcgaaggac tgccattacc ggacagggaa gatgcgcctc attccagaat
    19261 taaacaatta gcgagatttg cgcatacgca tcccggaggc ccgacatgtc actttaacga
    19321 cattattcca ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc
    19381 tctggcaacg aatcatcaaa cacagtacgg caccgttggc caggcactgc atattgcacg
    19441 gaaattactg cccttcattc ctgataatgc agggattctt atcgttccgt gttgccgtgg
    19501 cggatcggct tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca
    19561 tgatgcttgt cgttggggaa cggatactcc gctgtaccag gatttagtca gcagaacgcg
    19621 agccgcactg gcaaaaaatc cgcagaacaa attcctcggc gtatgctgga tgcaaggcga
    19681 atttgactta atgaccagtg actacgcgtc acaccctcaa cactttaatc atatggttga
    19741 agcctttcgt agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcgcc
    19801 gtggttttgc ggcgatacca cctggtactg gaaagaaaat ttcccgcatt cgtatgaagc
    19861 tatttatggc aattatcaaa ataatgtttt agccaatatt attttcgtcg acttccagca
    19921 acaaggtgaa agaggactga cgaacgcgcc tgatgaagac ccggacgatt taagcacggg
    19981 atattacggt tcagcgtacc ggtcaccgga gaactggacg acggcactgc gaagcagtca
    20041 tttcagcacg gcagcccgtc gggggattat ttctgacaga tttgtagaag caattttgca
    20101 gttttggcgc gaaaggtgag cggggagttt attcatgggg gatgtccttg ttcgttctca
    20161 gagtagacaa caaaaaacaa atgagatcaa ggtaatatct cttcacttgc gggctcgaac
    20221 agcaggcaat tgccaatata ataatctggc acaagggtga aaaacgcgca ccgcataaac
    20281 cgttgttatt gcttacagat tagccggatg ccttaataga catccgcgcc ttttcgatta
    20341 tggctcggaa atctacgaac atctacacag tttactggaa cgttttgggc cacagcgttc
    20401 gcagcaccgt cctgatatgc cgttctggcg attataagtc gacggattct ggcaaataga
    20461 cgatgatgat atgcttaatg atgttcaaag aaattaggcc attattatat tattgcttgc
    20521 attttctttc attcctaaaa gcacccgcca ttaatcccag gcgcattat
//
LOCUS       DAJVRP010000076        20495 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVRP010000076 DAJVRP010000000
VERSION     DAJVRP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.076"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(435..641)
                     /locus_tag="OQN12_002786"
     CDS             complement(435..641)
                     /locus_tag="OQN12_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS1655046.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="OQN12_002787"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="OQN12_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1655047.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="OQN12_002788"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="OQN12_002788"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS1655048.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="OQN12_002789"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="OQN12_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="OQN12_002790"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="OQN12_002790"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCS1655049.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="OQN12_002791"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="OQN12_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8714
                     /gene="kdpE"
                     /locus_tag="OQN12_002792"
     CDS             8037..8714
                     /gene="kdpE"
                     /locus_tag="OQN12_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCS1655050.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8989..9093
                     /locus_tag="OQN12_002793"
     CDS             8989..9093
                     /locus_tag="OQN12_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCS1655051.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9404..11601
                     /gene="speF"
                     /locus_tag="OQN12_002794"
                     /pseudo
     CDS             9404..11601
                     /gene="speF"
                     /locus_tag="OQN12_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11598..12917
                     /gene="potE"
                     /locus_tag="OQN12_002795"
     CDS             11598..12917
                     /gene="potE"
                     /locus_tag="OQN12_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS1655052.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="OQN12_002796"
     CDS             complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="OQN12_002796"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1655053.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="OQN12_002797"
     CDS             complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="OQN12_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1655054.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15369..16133
                     /gene="ybfF"
                     /locus_tag="OQN12_002798"
     CDS             15369..16133
                     /gene="ybfF"
                     /locus_tag="OQN12_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1655055.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16273..16566
                     /gene="ybfE"
                     /locus_tag="OQN12_002799"
     CDS             16273..16566
                     /gene="ybfE"
                     /locus_tag="OQN12_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1655056.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16706..17236
                     /gene="fldA"
                     /locus_tag="OQN12_002800"
     CDS             16706..17236
                     /gene="fldA"
                     /locus_tag="OQN12_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1655057.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17446..17532
                     /gene="uof"
                     /locus_tag="OQN12_002801"
     CDS             17446..17532
                     /gene="uof"
                     /locus_tag="OQN12_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1655058.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17525..17971
                     /gene="fur"
                     /locus_tag="OQN12_002802"
     CDS             17525..17971
                     /gene="fur"
                     /locus_tag="OQN12_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1655059.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18055..18381)
                     /locus_tag="OQN12_002803"
     CDS             complement(18055..18381)
                     /locus_tag="OQN12_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655060.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="OQN12_002804"
     CDS             complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="OQN12_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCS1655061.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4768 a         5106 c         5419 g         5202 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggagttacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ctactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta gccgcccgcg
     8461 tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc ctgctggcgg
     8521 tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac caggtgtggg
     8581 ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat ctgcgacaaa
     8641 aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt attgggtatc
     8701 ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa tttattatta
     8761 aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat ataaaacacg
     8821 aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat aattaatgat
     8881 ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat
     8941 ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat ggaaaataat
     9001 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
     9061 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt
     9121 acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata
     9181 ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga
     9241 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
     9301 atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt ctcctttgag
     9361 aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag aattaaaaat
     9421 tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatattga
     9481 cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg ttgaacgtgg
     9541 aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg caacggaaaa
     9601 tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg agcattgcga
     9661 atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt atgaaacgca
     9721 actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca atagcgcttt
     9781 tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag ggaatcagtt
     9841 cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag acgtagcaat
     9901 gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt
     9961 gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta acaaagtggt
    10021 tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata accacaaatc
    10081 taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg aaacggcacg
    10141 taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg
    10201 tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
    10261 tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg tggtggataa
    10321 aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct atgagcaatt
    10381 tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg
    10441 tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat
    10501 ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
    10561 caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga
    10621 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
    10681 aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt
    10741 acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga
    10801 tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga
    10861 gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg
    10921 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
    10981 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc
    11041 agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga aaaactgctt
    11101 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
    11161 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgccac
    11221 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
    11281 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
    11341 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
    11401 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc tctggaagaa
    11461 gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
    11521 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg
    11581 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa
    11641 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
    11701 ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc tcaatggcac
    11761 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt atgggcggtt
    11821 atgccgagta tgctttcggt aaatccggta actttattgc gaactatacc tacggcgtct
    11881 cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
    11941 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
    12001 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
    12061 gggggggcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
    12121 cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca gtaggttctt
    12181 ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg
    12241 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
    12301 cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat atggagctgg
    12361 caaattcaac ggcaccattt ggcctggcct tcgcacagat gtttacacca gaagtgggta
    12421 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
    12481 ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc
    12541 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg
    12601 ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg ctggttaacc
    12661 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
    12721 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg
    12781 gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg ctgtacggtt
    12841 ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc tttgaactga
    12901 aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc gctctttttt
    12961 tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc
    13021 aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc
    13081 cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccaat
    13141 agaagcaccg ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac ctgccagggt
    13201 gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc
    13261 agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg
    13321 ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac acataatgat
    13381 gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct
    13441 ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc atttgaaacc
    13501 taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga tcatcgcaga
    13561 agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat gctggaacag
    13621 gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat
    13681 accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc
    13741 cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac cgtctttatc
    13801 gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca ggttgaggtt
    13861 gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg gatcaacgcc
    13921 cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca gcccttccac
    13981 gaacggctgc accagatcct gctctttcac atagccggat gccatcgctt cgtcgaggga
    14041 gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccctgt cttccaccac
    14101 tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga taccaccatc
    14161 ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc cacctttttt
    14221 attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat tgttttcctg
    14281 cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga acgcaggctc
    14341 ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg
    14401 ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc
    14461 tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc
    14521 tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg
    14581 tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt
    14641 agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt
    14701 gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc
    14761 ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg
    14821 caaagtacac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt
    14881 caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg
    14941 cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga
    15001 ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag
    15061 caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc
    15121 cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt
    15181 tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta tatgaataaa
    15241 tcccggcagc ggatagcgtc aagcttggct tgcagaaaaa ctcggaataa accttatttg
    15301 ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga
    15361 actttgccat gaaattaaat atccgcgcgc aaactgcaca aaaccagcac aataattctc
    15421 ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta ctggctcgcg
    15481 atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga
    15541 gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc ctggatgcac
    15601 tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg gtaatggcac
    15661 ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg
    15721 actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc agtgaatcgg
    15781 acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat gaagaagggg
    15841 tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat
    15901 tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg
    15961 ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt gatgatttac
    16021 tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg
    16081 ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact
    16141 gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg
    16201 ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa
    16261 gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac
    16321 gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt
    16381 aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag
    16441 ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa tatgagccgt
    16501 agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc
    16561 gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca
    16621 agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa
    16681 taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac
    16741 cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga
    16801 tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttacgaca ttctgctgct
    16861 gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact tcttcccgac
    16921 tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg gtgaccagga
    16981 agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg
    17041 cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg
    17101 tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact
    17161 gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga
    17221 aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa
    17281 tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtacctg tacaatgtcc
    17341 cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata
    17401 tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc
    17461 tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt
    17521 ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg
    17581 tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg cggaagattt
    17641 atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact
    17701 gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt
    17761 atttgaactg acacagcaac atcaccacga tcacctgatt tgcctcgact gcggcaaggt
    17821 tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg
    17881 cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag gcgattgccg
    17941 cgaagatgag cacgcgcacg aaggcaaata agccagcctg aacgagaaaa gccaacctgc
    18001 gggttggctt ttttatacaa gggaaataag aaaagcgaga gttacagctc tcacttattt
    18061 gttattactg cgtatttcct gccagacctt atcacaatcg gctttcaccg cctgatcatt
    18121 tccggtttgc gttgcacgca gacactgctg ataatccagt acgcgtacac tttcctggtc
    18181 ggcaaactgc tgatgttgtt tctctttctt cagcacgttt aacacgctct ggcaggcttc
    18241 aattttttct ggcgaacctt gcgcggtgtt gatacaggcg ctgtatgcct ctttcagacg
    18301 gctgtcttcc tgtggtgcgg ttgattgcgc acaagctacc agccccgatg ccatcatggc
    18361 gatgagaatc agttttttca ttgaatgcgg caggcacaaa acctgccgcg tcgggcatca
    18421 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
    18481 accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg tgaagtgcag
    18541 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
    18601 ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgccaggt
    18661 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
    18721 ccagtttttc aggtcataca tcgcactgaa gaagaccgct ttttcgccgt tagcgttgaa
    18781 gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt aggttggggt
    18841 catacgttgc aggaagtatc cctgctgact gtcagcctta atccaggtgc cttcgaggcg
    18901 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc
    18961 gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgggtaccat agaactggta
    19021 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    19081 atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat ttttgaagtc
    19141 gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt tctgataaaa
    19201 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    19261 caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct gataagtacc
    19321 cggcataaag ctccagtgcg gcgctaacag cgtttgaccg gttggctgaa tgtaacctgc
    19381 cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc ttataccgct
    19441 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    19501 cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag caatatcaag
    19561 gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc aggtggagtg
    19621 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    19681 ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    19741 cggcattgcc ataaagcccg acattgctgt aacaccggca atagccagcg ccagcgtact
    19801 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    19861 aaggtgaaca gcgaaaaaaa ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga
    19921 ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg
    19981 taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt aagtttgcgg
    20041 ttgaaaggct tgcagaagaa taaaaacgtg aaattccagc aagatgaaag cggttacatt
    20101 ttatttaatt atgttttgtg attttaaatt tattttatgg ataaattgcg aagcggaatt
    20161 aattcgcgaa gaaaagcaga aaaaacgccg cagagcgcgg cgtttgggat tgtttgctat
    20221 ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaagcacgca aattcaatat
    20281 attgcagaga tcatgtaggc ctgataagcg aagcgcatca ggcaatttag cgtttgaaat
    20341 ttattgtcta tttattgccg gatgcgacgt aaatgcctca cccggcttgg tagaacttaa
    20401 aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt
    20461 ttttcggccg tggaatggcg gctgtcgagg cagaa
//
LOCUS       DAJVRP010000077        20448 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVRP010000077 DAJVRP010000000
VERSION     DAJVRP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.077"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OQN12_002805"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OQN12_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1655062.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OQN12_002806"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OQN12_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCS1655063.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OQN12_002807"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OQN12_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1655064.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OQN12_002808"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OQN12_002808"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1655065.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OQN12_002809"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OQN12_002809"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1655066.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="OQN12_002810"
     CDS             4449..4814
                     /locus_tag="OQN12_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1655067.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="OQN12_002811"
     CDS             4964..5482
                     /locus_tag="OQN12_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS1655068.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="OQN12_002812"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="OQN12_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="OQN12_002813"
     CDS             6713..7195
                     /locus_tag="OQN12_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1655069.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDNYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OQN12_002814"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="OQN12_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1655070.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OQN12_002815"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="OQN12_002815"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1655071.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OQN12_002816"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="OQN12_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1655072.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OQN12_002817"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="OQN12_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1655073.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OQN12_002818"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="OQN12_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1655074.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="OQN12_002819"
     CDS             10939..11730
                     /locus_tag="OQN12_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1655075.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="OQN12_002820"
     CDS             11751..13037
                     /locus_tag="OQN12_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1655076.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OQN12_002821"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="OQN12_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1655077.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="OQN12_002822"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="OQN12_002822"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1655078.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="OQN12_002823"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="OQN12_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1655079.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="OQN12_002824"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="OQN12_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1655080.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="OQN12_002825"
     CDS             complement(16985..17275)
                     /locus_tag="OQN12_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1655081.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OQN12_002826"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="OQN12_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1655082.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="OQN12_002827"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="OQN12_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1655083.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="OQN12_002828"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="OQN12_002828"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="OQN12_002829"
     CDS             19137..20378
                     /locus_tag="OQN12_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1655084.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5288 a         5346 c         4791 g         5023 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacatgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacagattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acagacaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acattccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacaatt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gactggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgagccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaac
//
LOCUS       DAJVRP010000078        20256 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVRP010000078 DAJVRP010000000
VERSION     DAJVRP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.078"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="OQN12_002830"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="OQN12_002830"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1655085.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OQN12_002831"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OQN12_002831"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCS1655086.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2999)
                     /locus_tag="OQN12_002832"
     CDS             complement(1728..2999)
                     /locus_tag="OQN12_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1655087.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3182..4387)
                     /locus_tag="OQN12_002833"
     CDS             complement(3182..4387)
                     /locus_tag="OQN12_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1655088.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="OQN12_002834"
     CDS             complement(4487..4843)
                     /locus_tag="OQN12_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCS1655089.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="OQN12_002835"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQN12_002835"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OQN12_002836"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OQN12_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1655090.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="OQN12_002837"
     CDS             6773..9829
                     /locus_tag="OQN12_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1655091.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="OQN12_002838"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="OQN12_002838"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1655092.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="OQN12_002839"
     CDS             10336..10524
                     /locus_tag="OQN12_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1655093.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="OQN12_002840"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="OQN12_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1655094.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="OQN12_002841"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="OQN12_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1655095.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="OQN12_002842"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="OQN12_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1655096.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="OQN12_002843"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="OQN12_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1655097.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="OQN12_002844"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="OQN12_002844"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1655098.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="OQN12_002845"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="OQN12_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1655099.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="OQN12_002846"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="OQN12_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1655100.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OQN12_002847"
     CDS             complement(17800..18036)
                     /gene="lpp"
                     /locus_tag="OQN12_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1655101.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OQN12_002848"
     CDS             complement(18347..19759)
                     /gene="pykF"
                     /locus_tag="OQN12_002848"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1655102.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5306 a         4856 c         5169 g         4925 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacaccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa actgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt ttgctgttca tttgcggtaa ttcattagct
     2401 acgctggcgt ccaccaactg gctggggaat ttgcttttca acagcatcag cgtgatcagt
     2461 acaaacagaa tacccggaat aatcagccca tagccgtacc agatattatt gagcaacata
     2521 tagctcacca gcattgggta gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg
     2581 accgccgaac cagaggcttt cggaaagcat tccatgagcg cggggtagcc acccgtatcc
     2641 agcgctgagt tagcgatacc tacgcacact gccagaccgt aggcgagagt taaattcggg
     2701 caagcgggaa taccaaagaa gaatagcaga tacattatta ctgccattaa tatcaccgcc
     2761 cgacgaccaa acttatcgga gatcacaccg aagaataaaa tactgatcaa tcgccccaaa
     2821 ccgataccgg aaattaaata ggcaatgccc gcgttgtcag tggaaaactt ttctgccaga
     2881 gatgtcatat tttgggcaag cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa
     2941 atacaaagaa cagccaggat aaatggcgtg ctgaaagata atttcgttac cgtacgtatt
     3001 gttctccgac ggtttacata attactatac tgaagatgat tttccctggg tacatggcat
     3061 aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt
     3121 agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc
     3181 attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc
     3241 gtaagccgat aaacagtgcc agtaaaaaac cgatggcagt atcgaaccac ataatatcgg
     3301 caatacttct ttgggacaga tgagccgtaa tcaacggaat agtaaaggtc gcaatactgc
     3361 ccgtactgta atagatccct gtagctttac ctttagcgta agggaaacgt tcagccatta
     3421 acgtcaggcc aatttgcaca acacctccag cagaggtaaa accaatgaca aaagcaaata
     3481 ttatcaccac ataaaatgtg ggatgcaggc agacggtaaa cagagcaata aatgagataa
     3541 aggtgtacag catcagtaat gttgttgggc gaacggtatt acgaatgagt ggagcggtaa
     3601 taaatacaca aagcagcgaa cccacggtgt agatactgag tagtttgatc gacatagtgt
     3661 atgacatgcc tgcaacaaat tgtccgtact gtgccagcca ctggctaacc agataaaacg
     3721 ttgccattga gatatagcca tataaggtat aactggctaa atcgataatt gaacagcgat
     3781 gttccgtaga gctggtggtt ttctttatga caggtaagcg acgacccgga tggggtggga
     3841 acgtacaacg gtataaaaac agagcgttaa taaacataat gcctgcagca atcataaagg
     3901 accaaccgaa ccacagttca gcccacacta acaggctaat gattagcggt aataaaaatt
     3961 gtccgctgga aacaaatgct ttaattaaaa tattggctgt cccaggtgag cgtggaaaag
     4021 cttccatcaa actgggataa gtgcctgcat cgagaaaact gtttgccatt cccgccagaa
     4081 agccaaaaac ataagcgata atgatgttat tggtctgcag gatgtcaaaa aagaaggcca
     4141 tatagcagca catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc
     4201 ctgcaaaaag caagacactt aatcgaccaa tgcccagcga tgagataact atcgagacac
     4261 ccgcggcatt agtctgccaa agtgtctcca gcgaggccat attcaggctc atcaaaatga
     4321 cgcccatacc atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggaaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggtggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcaacg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc ctggcacccg gtgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa ggtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gactgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gaatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagcctg gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaagtcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac cttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga ttccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca taacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggag cgtctggtga ccggcctgca acgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcagcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    18301 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    18361 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacgacgt
    18421 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
    18481 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
    18541 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
    18601 ctgcgcgagc agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt
    18661 cagcagtttc aacggcacca cggcataccg cttcggtgat gcgcagttta cggttgtcat
    18721 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
    18781 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt
    18841 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
    18901 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
    18961 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
    19021 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
    19081 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    19141 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    19201 ctacgccttt ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    19261 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtca ccgttgttca
    19321 gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    19381 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    19441 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    19501 ctttcagaga aacatcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    19561 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    19621 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    19681 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    19741 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    19801 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    19861 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    19921 cgtgagtgtc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    19981 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    20041 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    20101 aaggttacgc aaaaacttcg ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    20161 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    20221 aatttatcgc gttatttgtc tgattttgtt tgattt
//
LOCUS       DAJVRP010000079        20256 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVRP010000079 DAJVRP010000000
VERSION     DAJVRP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.079"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            113..1168
                     /gene="phoE"
                     /locus_tag="OQN12_002849"
     CDS             113..1168
                     /gene="phoE"
                     /locus_tag="OQN12_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1655103.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1207..1608)
                     /gene="crl"
                     /locus_tag="OQN12_002850"
     CDS             complement(1207..1608)
                     /gene="crl"
                     /locus_tag="OQN12_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1655104.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1666..2910)
                     /gene="frsA"
                     /locus_tag="OQN12_002851"
     CDS             complement(1666..2910)
                     /gene="frsA"
                     /locus_tag="OQN12_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1655105.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3002..3460)
                     /gene="gpt"
                     /locus_tag="OQN12_002852"
     CDS             complement(3002..3460)
                     /gene="gpt"
                     /locus_tag="OQN12_002852"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1655106.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3722..5179
                     /gene="pepD"
                     /locus_tag="OQN12_002853"
     CDS             3722..5179
                     /gene="pepD"
                     /locus_tag="OQN12_002853"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1655107.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5236..5850)
                     /locus_tag="OQN12_002854"
                     /pseudo
     CDS             complement(5236..5850)
                     /locus_tag="OQN12_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5912..6955)
                     /locus_tag="OQN12_002855"
     CDS             complement(5912..6955)
                     /locus_tag="OQN12_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCS1655108.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7201..7653)
                     /locus_tag="OQN12_002856"
     CDS             complement(7201..7653)
                     /locus_tag="OQN12_002856"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1655109.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7663..8061)
                     /gene="yafO"
                     /locus_tag="OQN12_002857"
     CDS             complement(7663..8061)
                     /gene="yafO"
                     /locus_tag="OQN12_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCS1655110.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8064..8357)
                     /gene="yafN"
                     /locus_tag="OQN12_002858"
     CDS             complement(8064..8357)
                     /gene="yafN"
                     /locus_tag="OQN12_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCS1655111.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8409..9464)
                     /gene="dinB"
                     /locus_tag="OQN12_002859"
     CDS             complement(8409..9464)
                     /gene="dinB"
                     /locus_tag="OQN12_002859"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS1655112.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9535..10320)
                     /gene="lafU"
                     /locus_tag="OQN12_002860"
     CDS             complement(9535..10320)
                     /gene="lafU"
                     /locus_tag="OQN12_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS1655113.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10292..12004
                     /locus_tag="OQN12_002861"
                     /pseudo
     CDS             10292..12004
                     /locus_tag="OQN12_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12221..12718)
                     /gene="rayT"
                     /locus_tag="OQN12_002862"
     CDS             complement(12221..12718)
                     /gene="rayT"
                     /locus_tag="OQN12_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCS1655114.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12894..13415)
                     /locus_tag="OQN12_002863"
                     /pseudo
     CDS             complement(12894..13415)
                     /locus_tag="OQN12_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            13799..14539
                     /locus_tag="OQN12_002864"
     CDS             13799..14539
                     /locus_tag="OQN12_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1655115.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14510..15277)
                     /locus_tag="OQN12_002865"
     CDS             complement(14510..15277)
                     /locus_tag="OQN12_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1655116.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(15483..16061)
                     /gene="lpcA"
                     /locus_tag="OQN12_002866"
     CDS             complement(15483..16061)
                     /gene="lpcA"
                     /locus_tag="OQN12_002866"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1655117.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16301..18745
                     /gene="fadE"
                     /locus_tag="OQN12_002867"
                     /pseudo
     CDS             16301..18745
                     /gene="fadE"
                     /locus_tag="OQN12_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(18788..19234)
                     /gene="ivy"
                     /locus_tag="OQN12_002868"
     CDS             complement(18788..19234)
                     /gene="ivy"
                     /locus_tag="OQN12_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1655118.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19415..20185
                     /gene="yafV"
                     /locus_tag="OQN12_002869"
     CDS             19415..20185
                     /gene="yafV"
                     /locus_tag="OQN12_002869"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1655119.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
BASE COUNT         4943 a         5211 c         5203 g         4899 t
ORIGIN      
        1 gaatatcatt aatctgtaat atatctttaa caatctcagg ttaaaaactt tcctgttttc
       61 aacggggctc tcccgctgaa tattcgcgcg ttaattaaaa tcaggaatga aaatgaaaaa
      121 gagcactctg gcattagtgg tgatgggcat tgtggcatct gcatctgtac aggctgcaga
      181 aatatataat aaagacggta ataaactgga tgtctatggc aaagttaaag ccatgcatta
      241 tatgagtgat aacgccagta aagatggcga ccagagttat atccgttttg gttttaaagg
      301 tgaaactcaa attaacgatc aactgactgg ttatggtcgg tgggaagcgg agtttgccgg
      361 aaataaagcg gagagtgata ctgcacagca aaaaacgcgt ctcgcttttg ccgggttgaa
      421 atataaagat ttgggttctt tcgattatgg tcgtaacctg ggcgcgttgt atgacgtgga
      481 agcctggacc gatatgttcc cggaatttgg tggcgattcc tcggcgcaga ccgacaactt
      541 tatgaccaaa cgcgccagcg gtctggcgac gtatcggaac accgacttct tcggcgttat
      601 cgatggcctg aacttaaccc tgcaatatca agggaaaaac gaaaaccgcg acgttaaaaa
      661 gcaaaacggc gatggcttcg gcacgtcatt gacatatgac tttggcggca gcgatttcgc
      721 cattagtggg gcctatacca actcagatcg caccaacgag cagaacctgc aaagccgtgg
      781 cacaggcaag cgtgcagaag catgggcaac aggtctgaaa tacgatgcca ataatattta
      841 tctggcaact ttctattctg aaacacgcaa aatgacgcca ataactggcg gctttgccaa
      901 taagacacag aactttgaag cggtcgctca ataccagttt gactttggtc tgcgtccatc
      961 gctgggttat gtcttatcga aagggaaaga tattgagggc atcggtgatg aagatctggt
     1021 caattatatc gacgtcggtg ctacgtatta tttcaacaaa aatatgtcag cgtttgttga
     1081 ttataaaatc aaccaactgg atagcgataa taagctgaat attaataatg atgatactgt
     1141 cgcggttggg atgacgtatc agttttaatg aatattgccg gatgtgatgc atccggcaca
     1201 tttcactcac gccgttaact tcaccggttc atcacgaaaa tcattcgccg gttccagctt
     1261 cagattcagc gtcgtcagca gttcacgcag cttctcgtga aactcacgca gggtgtgctc
     1321 cagtcgttca accacttcag tatcttttac cggaacactc ttccagtcgc ctgctttatc
     1381 gaacagacca aactggtagc tgtaggtaaa acgggattcc tgcgcttcca gctccatcca
     1441 ccagccccag aattcacgca cttccggtgc cggtttcacg ttgacgcata cagccagaca
     1501 atcgaaaaag aatcgattat cttcgcactt accttcacga atatacgggc ctagtgcggt
     1561 aaattttttg atcaatctgc tcttcgggtg tccactcggt aacgtcattg ctatctcctg
     1621 ttgtgatgca actgttttac caaattggca aaatttagca aatttttaac acaagcgttt
     1681 ttcgatccag ccggtgattt cctgaagacc tttgtcaaaa ttccgataca ccgggttaaa
     1741 tgggatctct aataatttac cgtcagcaga tgatgaggtg attaagcgtg agtcctcttc
     1801 cgggctgaac ggatcgttct tccagtagcc tgataacatt ggcgttgggc agcgacgccc
     1861 cagcaatcct tgcactttta atgaatagcg attcagctcc acgcgtaacg cttcgtcgga
     1921 agcatcatgc atccccaaac gactcgccag aacgtcaaga tacatttccg gcacctgttg
     1981 ctggcactta aaatcactca acagggtatg aactaccgga ccaagacagg caaccgcttt
     2041 cagacgcggc gattcaaggt atgccagacg cacggcaacg ttagcgccga aacggaaacc
     2101 aaaggccgcg acgcgagtgt gatccaccca cggtacgtta ggcagcgcct ttaagacgtg
     2161 ctgatgcaac aggctggagt cctgggtgag cttccatttt gaagaaaagc ccaccgacgg
     2221 catatcaata gtcagcatcg caatgccgcg cggcgcaaaa taacgttcat acaggctgta
     2281 atagtccgtc tgcatcgcat ccagaccacc acacattaat actgtcggga acgggccatc
     2341 gcctttcggc atatgcaaaa agccggtgat gggcgcaccg ccgggtacgg taaactccat
     2401 ctgccgcatc gtgcccggca gacgctgggc ggcctcttca taggcgcggt ttgataaagc
     2461 ctgcgcttgc tcggccaggt catctccttt cagatgagga taggcggcaa tgttgtacaa
     2521 cgtagccgca tgcagccagt gtcgaccact gagtttcgga tcttcttccg tacaggcttt
     2581 ttgttgccac accatcgcct gggtggccca ctcataaatc cagttgccac cacggtagcc
     2641 aatcacggta tcgtataaat catcgtcggt acgttcagca tcgctcatca caatacgtgc
     2701 ctggacgtcg aggatttcac gtgggtcaat accgcgccag atccacatca gacggttaat
     2761 catgcgatac cagtgaggga tggttttacc atcaagagcc gactgcacag gcggttgtgc
     2821 gccgtgatta aagcggcgga ctagcgtcga ggtttcagga tgtttaaagc ggggtttgaa
     2881 cagggtttcg ctcaggtttg cctgtgtcat ggatgcagcc tccagaatac ttactggaaa
     2941 ctattgtaac ctgcctgaag ttaaaaagaa caacgcccgg caatgccagg cgttgaaaag
     3001 attagcgacc ggagattggc gggacgaata cgacgcccat atcccacggc tgttcgatcc
     3061 aggtatcttg cgggatatca acaacatagt catcaaccag cggacgacca gccggttttg
     3121 cgaagatggt gacaaagtgc gcttttggat acatttcacg aatcgcaaca gcagtaccac
     3181 cggtatccac caggtcatca ataacgatga agccttcgcc atcgccttct gcgcgtttca
     3241 gcactttaag ctcgcgctgg ttgtcgtgat cgtagctgga aatacaaacg gtatcgacat
     3301 gacgaatacc cagttcacgc gccagtaacg cccccggtac cagaccgcca cggcttacgg
     3361 caataatgcc tttccattgt tcagaaggca tcagtcggct tgcgagttta cgtgcatgga
     3421 tctgcaacat gtcccaggtg acgatgtatt tttcgctcat gtgaagtgtc ccagcctgtt
     3481 tatctacggc ttaaaaagtg ttcgagggga aaataggttg cgcgagatta tagagatctg
     3541 gcgcactaaa aaccagtatt tcacatgagt ccgcgtcttt ttacgcactg cctctccctg
     3601 acgcgggata aagtggtatt ctcaatacat atctcgcaag cctgtcttgt gttgacaaca
     3661 ttttctgcta accctgtgac ctgcaatact gttttgcggg tgatcgacaa ggagacttaa
     3721 cgtgtctgaa ctgtctcaat tatctccaca gccgctgtgg gatatttttg ccaaaatctg
     3781 ttctattcct cacccgtcct atcatgaaga gcaactcgct gaatacattg ttggttgggc
     3841 aaaagagaaa ggtttccatg tcgaacgcga tcaggtaggt aatatcctga ttcgtaaacc
     3901 tgctaccgca ggtatggaaa atcgtaaacc ggtcgtctta caggcccacc tcgatatggt
     3961 gccgcagaaa aataacgaca ccgtgcatga cttcacgaaa gatcctatcc agccttatat
     4021 tgatggcgaa tgggttaaag cgcgcggcac cacgctgggt gcggataacg gcattggtat
     4081 ggcctctgca ctggcggttc tggctgacga aaacgtggtt cacggcccgc tggaagtgct
     4141 gctgaccatg accgaagaag ccggtatgga cggtgcgttc ggcttacaga gcaactggtt
     4201 gcaggctgat attctgatta acaccgactc cgaagaagaa ggtgaaatct acatgggttg
     4261 tgcggggggt atcgacttca cctccaacct gcatttagat cgtgaagcgg ttccagctgg
     4321 ttttgaaacc ttcaagttaa ccttaaaagg tctgaaaggc ggtcactccg gcggtgaaat
     4381 ccacgttggc ctgggtaatg ccaacaaact gctggtgcgc ttcctggcgg gtcatgcaga
     4441 agaactggac ctgcgcctta tcgatttcaa cggcggcaca ctgcgtaacg ccatcccgcg
     4501 tgaagccttt gcgaccattg ctgtcgcagc tgataaagtc gacgcactga aatctctggt
     4561 gaatacctat caggagatcc tgaaaaacga gctggcagag aaagagaaaa atctggcctt
     4621 gttgctggac tctgtagcga acgataaagc tgccctgatt gcgaaatctc gcgatacctt
     4681 tattcgtctg ctgaacgcca ccccgaacgg tgtgatccgc aattccgacg tggcaaaagg
     4741 tgtggtcgaa acctccctga acgtcggtgt ggtgaccatg actgacaata acgtagaaat
     4801 tcactgcctg atccgttcac tgatcgacag cggtaaagac tacgtggtga gcatgctgga
     4861 ttcgctgggt aaactggctg gcgcgaaaac cgaagcgaaa ggcgcatatc ctggctggca
     4921 gccggacgct aattctccgg tgatgcatct ggtacgtgaa acctatcagc gcctgttcaa
     4981 caagacgccg aacatccaga ttatccacgc gggcctggaa tgtggtctgt tcaaaaaacc
     5041 gtatccggaa atggacatgg tttctatcgg gccaactatc accggtccac actctccgga
     5101 tgagcaagtt cacatcgaaa gcgtaggtca ttactggaca ctgctgactg aactgctgaa
     5161 agaaattccg gcgaagtaat tatttgattt gctgccggat ggcgtttaat cgccttccgg
     5221 cagtttcatc cttcattatc cttcgataaa agccatccct gtaaacgttc gtcgcgggtt
     5281 gccacgttca atctggtgat ggaacattcg ccgctgtgat ttcagcaccg cgctattttc
     5341 ctattgctgc tgatccagct tccaggcaat cagcaatcgc gccagccgtt tgttagcatg
     5401 ctggctgcgc tctgactgaa ccttcacgct aataccggat gccaaatgcg tggcgcgtac
     5461 cgccgagtcg gttttattga catgttgacc gcccggcccc gacgaacgca gcgtctcata
     5521 ccggatcgca tcggattgct cctgctcatc agcggtaaaa cgcccaatgc ccagaaacca
     5581 gtttttacgc ccgtgatgtg ggcgatacgg gctcgggcaa atccactgaa tagtgccaca
     5641 ccagctttcg cttaacgccc atgcgttgtc gccatccaga gaaatcagcg ccgaacgcag
     5701 cgtgtcagag tagcggcctg tttctgtttc cagcaccgtt accgcgacgt cttgtcgggc
     5761 agcttcttta attagcctgt ccagcgcctt tttcactgcg agacaacatt cttccggccc
     5821 ctgcgctgag gagagttata gcaagatcat ccttttttcc ctccactgtt tttgagcgtt
     5881 agcaccggac gcagccgcgc gacaggaata attaatccgg cctgcccaga cattgcacca
     5941 cactttcagc cgatttatag gcttgtggcg cttcttcgaa gatgagttgt ttatcgcgac
     6001 aaattacccg actgccaagt tcagtacgcg agagttgcgt cgctgtgtat ttcgctgcca
     6061 gacgcccttt acactcggtg cgcccccatt tacgcccagc cccatgcgcc agcgaatgca
     6121 atgtttcctc actcctgacg ggctgaacca gcctggagta atcaccgcgt gaaccgggaa
     6181 taaccaccag accacagtca tcaggcgtgg cacctttacg atgtaagacc ccttgttgat
     6241 caccgattcg acacgcgcta acaaagttat gcgccacatc cagaaccgga cttccggtgg
     6301 ccttaacctg ttgcattatg cgcaaagcga tcaggtggcg attaaaacgc gcaaacgcca
     6361 gcgcgtcgtc actgccttca ggcaaaccat gatgcgaaaa cgaggcaata tgccgctgta
     6421 aaatagactg ccctagcccc ctcgagccgc tatgaaccag cagttgcaga tgctgcgcat
     6481 ccagacctgc cagtgcaaac agttcagcgt tgatgatttg atcaacttgt tgcagttcag
     6541 cgaaatgatt gccaccaccg atagagccaa gcgagctgcg ccacggatgc tgtgcaaatg
     6601 ctgacggcag gttttcctcc agccagcttt cttcagcaac gtcatccaaa gctgataatc
     6661 gcttttcaaa cttatcggcg ttgtatttgc gagcgagaat atctgtttgc catagcgcca
     6721 tgccacagcc gatatcattg ccgaccagtg ctgggtaaaa acggcctgcg gagaagaacg
     6781 ctgcgccaat cgggtagccg cgtccggggt gtaaatctgg catccccact acgcgctgca
     6841 tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc taaactctcg atccacaggt
     6901 catcagatgc gatggtaaat accgcatcgg ataagggacg aatataattg cccatatacc
     6961 attccgtaaa tagactcgtc atacttcaag ttgcatgtgc tgcgacttga attattgaga
     7021 gtaacaatta aaaaatcagg aaatggcaga cgaatccagg gcaagaaaaa aagaattaat
     7081 cctggaaagg tctgcaaaga gtcattgata aaggatttgt catgatttac ctccttgcag
     7141 tttgtggaat aagaaaacgc gcacatacta ggcgggcgtt attttcattg caagctggat
     7201 ttaatgttgc ggtttataac gcatataaaa attaataaac cacgctcccc ggcattcaac
     7261 gcgctgctgc ttaactgtct gaaaaccata acgttcaaaa aagggttttg cggttatgct
     7321 tgcgtccacc gtaagttcgg attcagactt aataaaaggt tttaacaaag cgctggcaac
     7381 cccacggcgg gtgtattcag ggtcaacaaa taacatatcg atatagtgtt caacgcaggt
     7441 aataaagccg accagttttg cattaataac tgcaacccgc acttgtgatt tcgcgagttt
     7501 ctccttccag cgagattcgt caatctgcgc ccaggcggca atttgttgta gtgaataatg
     7561 ctgactgacg gtcatcgtaa ccgctctaat gaaaatagcg catagttgct gaaaatcgcc
     7621 aggatgataa tctcttattt gtatgttatt cataatataa attcaaaaac gcatgcgaaa
     7681 cgcttctgcc atttttccaa ttttatgcat ttggttgtta tctcgagcca gtttatgagg
     7741 ttcaggcttc agaatggcaa tgagcaacca tgcttgctca tcaaacgcac cctgacaata
     7801 taccaaatgc gcttcgtcat tcgttctgct gaattggcgc aactgtggcg gaaatggatt
     7861 attcacattt gccagatgaa tatgagcaac tcgctcaaat ttgattaatg gccaggtaaa
     7921 cgagtcgtcg tagagtgcat cgcgaccaaa tatatctggc aaaacaccgt cacgcttata
     7981 ggaaataaaa tccgccgtta acgcatcaag ttcctctgct gtaagttgca ggcgaataag
     8041 ttttgttttg aatacccgca tccttattcc ttaaagtcat tgaaatcatc atccgtcata
     8101 tcattaagat attgtttagc gcggcgtgta atttcctcta atcttgcagc acttggacga
     8161 aagcgcgcct ttatgggctt tttctcctct tgttcagcaa gcatgtccat taacgcttcg
     8221 aatgcgctgg cacttaagag atatcctgcg gggcgattat tagaaagaac cgcaactggt
     8281 tgatcaataa agtatttagc tgggttttta cgtaactcag tgatattgac cgatttttca
     8341 gcgagaattc gatgcataca gtgataccct cataataatg cacaccagaa tatacataat
     8401 agtatacatc ataatcccag caccagttgt ctttccattt gcgggtcaag caacgtcaca
     8461 tgcagcccca ccagacgcac accgcgcccg ccgcggcgtt catcccaggt tttacgcgcg
     8521 gtggcgatta agtcagcttt attcagccgc ggccagacgt gctcctgggt ggtctgctga
     8581 aaatcgtcga actttaattt caccccctgg cgagcaatca gtaaatcagg ttttaccttt
     8641 gccagacggc gttcaagttc cggatacagc cgctcgataa tcgcttcaca ttcagaccag
     8701 tgatgaatat cttccgccat cgtgcgttcc acgccgacgg attttcgtaa ccgttcgctg
     8761 ttaacgtcgc gctcgtcaat cccctgacta cgctcccaca aaatgcggcc aaatttgcca
     8821 aagcgtttaa gcagaatcac cagatcacac ttttgtacat caccgcaggt ccgcagcccc
     8881 atcgcttcca gttttgccgc cgagactttg ccgacgccgg ggattttttc cagtggtaag
     8941 gtttgtaaaa atgccggaac ttctgccggc gtaatcacaa actgaccgtt gggtttattc
     9001 atgtcggagg cgattttggc gagaaacttt actggtgcca cgcccgcaga tgccgtcagt
     9061 tgcagctcgt tgaagatcgt ctggcggatt tcctgggcga tgagggtcgc agaaccgtgg
     9121 caatggacgc tatcggtgac atcgagataa gcctcatcca gtgacaacgg ttcaatgcgc
     9181 gaggtgtagc gcgagaagat ttcacggata tgatttgagg cttctttgta ggcgtcaaag
     9241 cgccccggaa gcaaggtgag atgtgggcat aatttgagcg ccatcccagt aggcatagcg
     9301 ctacgtacgc caaatttacg cgcgggataa ttggcggtgc tgatcacccc ccgacgttcg
     9361 cggctgccgc caatagcaat agggatatcg cgcagagcgg gattgtcgcg catctccacc
     9421 gctgcgaaaa agcagtccat atccacatga atgattttac gcattgctca cctctcaaca
     9481 ctggtaaagt atacagtgat ttcaggtttt gagaaatgcg taaagattca gcatttagcg
     9541 cattcgctgc gaaagcacct gctgcttatc cagcttttgc accagcggct gcaccacttt
     9601 atccccatgc tgaccaaagt attgatacag cgtatcggac gcacttttgg tcagcaccat
     9661 aatctcaata cgccgattgc ccgcgctttg cggattcttc gcgtccagca gcatctggtc
     9721 cgccattgcg cttacctgca tcactttatc ttccggcatt ccggcctctt ccagcacccg
     9781 acgagctgaa agcgcgcgat cgcccgaaag gttccagttg ttgtagatat tgtttttgta
     9841 ggccatcgca tcggtatgcc cggtaataat aattttgtta tagagcgagt cgaacactgg
     9901 cgcgagctcc accagcaaag ttttaaagaa cggcataatc tgcgcgctgc cgcgttcgaa
     9961 catattgcga ttctggtcgt ctttaatcag cacgcgtaat ccctggggaa caatctccat
    10021 ctccagattc gcttccatat gcgcatcacg ggcaatggtg ttgatactgg ttgccaactc
    10081 gccaagctcg gtggcggatt tcttcttcag cagcgccgtt ttctcattca catcacgcgc
    10141 ttttttctcc gtctcttccg gcaccgccac ggtggcaggt tttggcggat gagacgggct
    10201 gattttattt aacggcgaca gcccaccacc gttaaaaatc gactgcccgt gcagcgcggc
    10261 gataatgctt tcacgctcgg atttactgac gctgttgaca atcaacttta tcgtcgtcac
    10321 caaagaggcc gagcgtattt ccgaggtttc tgcccgcttt accctggacg cgatgcccgg
    10381 caaacagatg gcgattgacg ccgatcttaa cgccggattg atcaaccagg cgcaggcgca
    10441 aacccggcgt aaagatgttg ccagcgaggc cgatttctac ggcgcgatgg acggggcatc
    10501 gaagtttgta cgcggagacg ccatcgccgg gatgatgatt ctggcgatca acctgagcgg
    10561 cggcgtctgt atcggcatct tcaaatacaa cctgagcgcc gacgccgcct tccagcaata
    10621 cgtgctgatg accatcggcg acggcctggt ggcgcagatc ccttccctgc tgctctccac
    10681 cgcggcggcg attatcgtca cccgcgtcag cgacaacggc gatatcgccc atgacgtgcg
    10741 ccaccagttg ctggcaagcc cgtcggtgct ctacaccgcc accgggatca tgttcgtgct
    10801 ggcggtggtg ccggggatgc cgcatctgcc gtttttgctg ttcagcgccc tgcttggctt
    10861 taccggctgg cggatgagca aacgcccgca ggcggcggag gcggaagaga aaagcctcga
    10921 aacgctgacc cgcaccatca ctgaaaccag cgaacaacag gtcagttggg aaactattcc
    10981 gctgatcgag cccatcagct taagcctcgg ctacaagctg gtggcgctgg tggacaaagc
    11041 gcagggcaac ccgctcaccc agcggattcg cggcgttcgg caggtgattt ccgacggtaa
    11101 cggcgtgctg ctgccggaga tccgcattcg ggaaaacttc cgcctcaagc ccagccagta
    11161 cgctattttc atcaacggca ttgaggctga tgaagcggat attccggcgg ataaactgat
    11221 ggcgctgccc tccagcgaaa cctacggcga gattgacggc gtgctgggga acgacccggc
    11281 gtacgggata ccggtcacct ggatccagcc tgcgcagaaa gcgaaggctc tgaatatggg
    11341 gtatcaggtg atcgacagcg ccagtgtgat tgccacccat gtgaacaaga ttgtgcgcag
    11401 ctatattcct gatttgttta actatgatga catcacgcag ttgcataacc gtttgtcgtc
    11461 gatggcaccg cgcctggcgg aggatttaag cgcggcgctc aattacagcc aattgctgaa
    11521 agtgtaccgg gcgctgctga ccgaaggcgt ttccctgcgc gatatcgtca ccatcgccac
    11581 cgtgctggtc gccagtagca cggtgactaa agatcatatt ctgctggcgg ccgatgtgcg
    11641 cctggcgctg cggcgcagca ttactcatcc gttcgttcgc aagcaggagc tgacggtgta
    11701 tacgctgaat aatgagctgg aaaatctgct gaccaacgta gtgaatcagg cgcaacaggc
    11761 cggcaaagtg atgctcgaca gcgtgccggt tgacccgaat atgctcaacc agttccagag
    11821 cacgatgcca caggtgaaag agcagatgaa agcggcgggg aaagatccgg tgctgctggt
    11881 gccgccgcag ctacgccctt tgctggcgcg ttatgcaagg ttgtttgcgc cggggctgca
    11941 tgtgctgtcg tataacgaag tgccggatga gctggagttg aagattatgg gggcgttgat
    12001 gtaagaatcc ggcatcggtg cacaattgcc ggatgcggcg taaacgcctt atccggccta
    12061 ccgttcaggc acaagttttt aggcatgata agacgcgcca gcgtcgcatc aggcatctgc
    12121 gcataactgc cggatgcggc gtgaacgcct tatccggcct accgttaaag cacgatttgt
    12181 aggccggata agcgtaagcg catccggcac cagcgcacag ttataaaatg atacgctccc
    12241 cggcacttaa atccgttatg tctcccgccc aatcgatggg atataaccct cgcgcgacat
    12301 cgcgatggaa cgttgagaat ggccaatcac tcacctgctt tacccagcca tgtttctctg
    12361 gatttatata aatataatca acatgatggc gataatcttt tgtattgcga atggcatgct
    12421 cccaaaaacg cggttgccag atatttttca atccgcaagc atgggtgaac tgctttttaa
    12481 tctcccgcca gcgagaggaa aaatcatcat cgctttcggg taatgtccat atgcaatgca
    12541 tatgctccgg caaaacgacc caggcgttaa tttcaaaagg tctgtctcgc ttaacgttaa
    12601 taatggcgtt acggagcgtc tggaactggg tggtcagaag atggcttcga cgattgcgta
    12661 aatttaccgt gaaaaaccat gttcccccct tgatgtaata acgacgatat tccgacatag
    12721 cgactccttt ttctcaacgg taagaggaaa aggaaaggcc gacaatgacg ctgtgttata
    12781 tcgggaaagc ggctcgcaaa aacgcacaga tttgtaggca tgataagacg cgtcagcgtc
    12841 gcatcaggca tccgtgcaca accgccggat gcggcgtaaa cgccttatcc ggcctacaaa
    12901 atcatctctt ccgtcaaaat cctgcgcgcg cctaaaaaat ggtcctgcca gaaaggttcg
    12961 gctaaccggc ttacccgaat ggtttccccg gtgcgcagtg actcaataaa ctgaccgtcg
    13021 cccagataca cccccatatg gtcggcaatc tcgcggctgt ggatatggaa aaatagcaaa
    13081 tctccccggc gcaggtcgtt attcgccaca atcgttgccc gacggtagtg gtacatctca
    13141 ttggccgtgc gcgggagttt cgcctcaagg atcttgttgt aggcataaaa aactaaccca
    13201 ctacaatcga agccttgatc gggccgcgtg ccgccccaga cgtaaggctt gccaagctgc
    13261 tgctccaggc ggtgaatcgc cacttcggtg atgttatgca ggtgatcgct gcttaaaaag
    13321 tggttgtttt ccgccagcgc ctgccagcgt ctgtcactcg ccttgagcgg cgcaggaaac
    13381 cattctggat gctgttttat ctgctcccgg ttgtggtggc gctggcgagt aaagggagca
    13441 aaacgcataa cgaaaacacg cctggatgca aaaatctgcc gagcaagaac gaagacatga
    13501 aagacataaa ccggctgaat gaaggtgaga attttaaaag accggaggag taaacgggat
    13561 tattaattgc ttatttgcat attactattg agatttcata atattaggaa ataaatgata
    13621 catgcgcttt tcggactatt gaaaactgcc cctgtgctaa atttgtaaac ccagcgccga
    13681 caggcttgcg taaaactctt ttggtgataa tgtgagcctt ccaaatccag actattccga
    13741 tttgggatcc attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa taagcagtat
    13801 gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg tgcgtttcgt ttgccggttt
    13861 gctgggcagc agtagctcga ccacgccagt gagcaaagag tataagcagc agttgatggg
    13921 atcccctgtc tacatccaga tcttcaagga agaacgtacg ctcgatcttt acgtcaaaat
    13981 gggcgagcaa tatcaactgc tcgacagtta taaaatctgt aaatattccg gcggcttagg
    14041 gccaaaacag cgtcagggcg atttcaaaag cccggaaggg ttttatagcg tccagcgtaa
    14101 tcagttaaaa ccagacagcc gttactacaa agcgattaat attggattcc ccaatgctta
    14161 tgaccgtgcg catggttatg aagggaaata cctgatgatc cacggcgatt gtgtatccat
    14221 cggctgctac gcaatgacca atcagggtat tgatgagatc ttccagttcg ttactggtgc
    14281 gctggtgttt ggtcagccga gcgtgcaggt gagtatttac ccattccgca tgaccgacgc
    14341 caatatgaag cgccataaat attccaactt taaggacttc tgggagcagc tgaagccggg
    14401 ctacgactac tttgagcaaa cccgtaagcc accgaccgtt tctgtggcca atggccgtta
    14461 cgtggtcagc aagccgttaa gccacgaagt ggtgcagcca caactggcat caaactacac
    14521 gctccccgag gcaaaataag tgccattcgc ctggcataat tttttgccag gtttcattgc
    14581 ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt cggtgtggtc tgcgagctga
    14641 agtcgatttc cacatcctga tccagcaacg ttgccacgcc aaacggcgcg cggcgggtga
    14701 tccagtgtaa attagtcgag caatacgcca ttacatagcg cccgtccgaa agcagcatgt
    14761 tgaaaacgcc cttcgcccgc agttcatccg ccagtgaggc gatatattta aacactgccg
    14821 ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt atgcagcagc cagcaaaagg
    14881 ctttttcgct gtcggtttca ccgaccgggc ggaagttgcc ggtttccagt gatttgtagc
    14941 ccgtcagttg tccgttatgg gcataagtcc agttacgccc ccataactcg cgggtaaatg
    15001 ggtgagtatt ttccagcgcc acctcgcccc gattagcctg gcgaatatga gccaccaccg
    15061 aacaggattt tatcgggtag tcctggacaa gtttggcgat gggggaatta aagctgggtt
    15121 gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc cagccatctt
    15181 tatgtggccc ggttccacca ccacgctgta caagtccggt gaaactaaag cagatatcgg
    15241 tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg taacctccac aacagcggga
    15301 gcgcatggac cccggcgaag attgatttaa gaattttcag gtcggatgcg gcgtaaacga
    15361 attattcggc ctacggttcg ggtacagatt tgtaggcatg ataagacgcg tcagcatcgc
    15421 atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg ccttatccgg cctacgccag
    15481 acttacttaa ccatctcttt ttcaattaac tggatcagga tatggatcac tttaatgtga
    15541 atctcctgaa tgcggtcggc ataaccaaag tgcggaacgc gaatttcgat atccgccgtg
    15601 ccagccattt taccgccgtc tttaccggtc agggtgatca ctttcatccc cttctcacgc
    15661 gccgctgcga tcgctttgat cacgtttgca gagttaccgg aggtggagat ccccagcagt
    15721 acatcgcctt cgcgacctac cgcttcaacg tagcgggaga aaatatcatt gaaaccgaaa
    15781 tcattaccga cgcaggaaat atgactaacg tcagaaatag caatcgccgg gtagcccgga
    15841 cggttttcac ggtagcgacc ggtcaactct tcggcaaagt gcatagcgtc gcaatgggaa
    15901 ccgccgttgc cgcaggaaag cactttgcca ccggctttaa agctgtctgc taacaggacc
    15961 gccgcgcgct gaatggcgtg aatattggcg tcatctttta aaaagttagc cagcgtttcc
    16021 gccgcttcgt tcagttcgtt acgaataaga tcctggtaca tgaggatatc cttcagcata
    16081 aatgtaatag acaaaatgca gtgtaccgga taccgccaaa agcgagaagt acgggcaggt
    16141 gctatgacca ggactttttg acctgaagtg cggataaaaa cagcaacaat gtgagctttg
    16201 ttgtaattat attgtaaaca cattgctaaa tgtttttaca tccactacaa ccatatcatc
    16261 acaagtggtc agacctccta caagtaaggg gcttttcgtt atgatgattt tgagtattct
    16321 cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat ttatcagcag
    16381 tctgattttg ctcgcctaga cagccgccct cggcgttgct ggtctgtggt cggcgtgggt
    16441 actggtgcct ctgaccatta tcctcgtgcc atttaacttt gcgcctatgc gtaagtcgat
    16501 gatttccgcg ccggtatttc gcggtttccg taaggtgatg ccgccgatgt cgcgcactga
    16561 gaaagaagcg attgatgcgg gcaccacctg gtgggagggc gacttgttcc agggcaagcc
    16621 ggactggaaa aagctgcata actatccgca gccgcgcctg accgctgaag agcaagcgtt
    16681 tctcgacggc ccggtagaag aagcctgccg gatggcgaat gatttccaga ttactcatga
    16741 gctggcggat ctgccgccgg agttgtgggc gtaccttaaa gagcatcgct tcttcgcgat
    16801 gattatcaaa aaagagtacg gcggactgga gttctcagct tatgcccagt ctcgtgtgct
    16861 gcaaaaactc tctggcgtga gcgggatcct ggcgattacc gtcggcgtgc caaactcatt
    16921 aggcccgggc gaactgttgc aacattacgg cactgacgag cagaaagatc actatctgcc
    16981 gcgtctggcg cgtggtcagg agatcccctg ctttgcactg accagcccgg aagcgggttc
    17041 cgatgcgggc gcgattccgg acaccgggat tgtctgcatg ggcgaatggc agggccagca
    17101 ggtgctgggg atgcgtctga cctggaacaa acgctacatt acgctggcac cgattgcgac
    17161 cgtgcttggg ctggcgttta aactctccga cccggaaaaa ttactcggcg gtgcagaaga
    17221 tttaggcatt acctgtgcgc tgatcccaac caccacgccg ggcgtggaaa ttggtcgtcg
    17281 ccacttcccg ctgaacgtac cgttccagaa cggaccgacg cgcggtaaag atgtcttcgt
    17341 gccgatcgat tacatcatcg gcgggccgaa aatggccggg caaggctggc ggatgctggt
    17401 ggagtgcctc tcggtaggcc gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa
    17461 atcggtagcg ctggcaacgg gcgcgtattc tcacattcgc cgtcagttca aaatctctat
    17521 cggtaagatg gaagggattg aagagccgct ggcgcgtatt gccgggaacg cttatgtaat
    17581 ggatgctgcg gcatcgctga ttacctacgg cattatgctc ggcgaaaaac ctgccgtgct
    17641 gtcggctatc gttaagtatc actgtaccca ccgcgggcag cagtcgatta ttgatgcgat
    17701 ggatattacc ggcggtaaag gcattatgct cgggcaaagc aacttcctgg cgcgtgctta
    17761 ccagggcgca ccgattgcca tcaccgttga aggggctaac attctgaccc gcagcatgat
    17821 gatcttcgga caaggagcga ttcgttgcca tccgtacgtg ctggaagaga tggaagcggc
    17881 gaagaacaat gacgtcaacg cgttcgataa actgttgttc aaacatatcg gtcacgtcgg
    17941 tagcaacaaa gttcgcagct tctggctggg cctgacgcgc ggtttaacca gcagcacgcc
    18001 aaccggcgat gccactaaac gctactatca gcacctgaac cgcctgagcg ccaacctcgc
    18061 cctgctttct gatgtctcga tggcactgct gggcggcagc ctgaaacgtc gtgagcgtat
    18121 ctcggcacgt ctgggggata ttttaagcca gctttacctc gcctctgccg ttctgaagcg
    18181 ttatgacgac gaaggccgta atgaagccga tctgccgctg gtgcactggg gcgtacagga
    18241 tgcgctgtat caggcagaac aggcaatgga caatttactg caaaacttcc cgaaccgcgt
    18301 ggttgccggg ctgctgaatg tggtgatctt cccgaccgga cgtcattatc tggcaccgtc
    18361 tgacaagctg gatcataaag tggcgaagat tttacaagtg ccgaacgcca ctcgttcacg
    18421 tattggtcgc ggtcagtacc tgacgccaag cgagcataac ccggttggtt tactggaaga
    18481 ggcgttggtg gatgtgattg ccgccgaccc gattcatcag cggatctgta aagagctggg
    18541 taaaaatctg ccatttaccc gtctggatga actggcgcac aacgcgctga cgaaggggct
    18601 gattgataaa gatgaagccg ctattctggt gaaagcggaa gaaagccgtc tgcgcagtat
    18661 taacgttgat gactttgatc cggaagagct ggcgacgaag ccggtaaagt tgccggagaa
    18721 agtgcggaaa gttgaagccg cgtaattgaa taacggagcc gaaaggctcc gtttctttat
    18781 ccgctaatta tttaaaatta aagccatccg gatggttttc caggctgccg gtcaacgccg
    18841 cgaacagcac cgttttacca tcaatcgaaa gcgcatcgtt cacattcagc caggtgagtt
    18901 tctcttgcga cgttttctca tcaatagccg agaacagccc cgtcatctga ttagatttct
    18961 cggaccacat cacagcgata cgttgcgagc cacagtcatg cggtttgcac gcgctcatca
    19021 cctgatacgt ctcatctccc aacgttacgg tttgtgcggg agtataagta ccgcctttca
    19081 tcacccatgc aggtagctta tgcccttgca ccatctgatt aaacgcagct ttggtggttt
    19141 cgccctttgc aaggctgcta atggttaaat catcctgcgc cattgcactg gtggcgatga
    19201 ccagtgcggc gactgtcgtt attgccttaa acatcattcc tcccgagctt atcctgccca
    19261 tgttattaac ctcctaaagc ctggcgataa cacgataatt tgcaaatcag atatttccaa
    19321 tatcagcatt tgttatcaat ttattctgcg ctaaggcaac tgcctgataa ggtgttaaag
    19381 ttataaaacc cttcgcataa aggagttgta caccgtgcct agtttgaaga ttacgctttt
    19441 gcagcaaccg ttggtgtggg tggatggtcc tgccaacctg cgccattttg atcgtcaact
    19501 ggaaggtatt accgggcgcg atgtgatcgt tctaccggag atgtttacca gcggctttgc
    19561 catggaagcg gcagcttcgt cgcttgcaca agatgacgta gtgaactgga tgacagctaa
    19621 ggcgcagcag tgcaatgcgc tgattgcagg cagtgttgca ttacaaacgg agtctggttc
    19681 ggttaaccgc tttttgctgg ttgagccggg cggcacggta catttttatg ataagcgtca
    19741 tctgttccgc atggcagatg agcatctgca ttataaagcg ggcaatgcgc gagtgattgt
    19801 ggaatggcgc ggctggcgta ttttgccgct ggtgtgctac gacttacgtt ttccagtgtg
    19861 gtcgcgcaat ctcaacgatt atgacctcgc cctgtacgtc gccaactggc cagctccgcg
    19921 ctctctgcac tggcaggcat tgctgacggc ccgcgcgatt gagaatcagg cgtatgtggc
    19981 gggatgcaat cgcgtcggca gcgatggcaa cggctgccat tatcgcggtg acagccgggt
    20041 gattaatccg caaggcgaga ttatcgctac cgccgacgcg cataaggcaa cgcgtattga
    20101 tgcggagctg tcgatggtgg cattgcggga atatagagaa aagtttccgg catggcggga
    20161 tgcggatgag tttaggttgt ggtgagtggg ggttgtgttt agggccgggg gctgggctag
    20221 attacgaaag cccctaactt aaacacaata ttacgc
//
LOCUS       DAJVRP010000080        20209 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVRP010000080 DAJVRP010000000
VERSION     DAJVRP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.080"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..1462)
                     /locus_tag="OQN12_002870"
     CDS             complement(<1..1462)
                     /locus_tag="OQN12_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HCS1655120.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQIL"
     gene            complement(1459..1773)
                     /locus_tag="OQN12_002871"
     CDS             complement(1459..1773)
                     /locus_tag="OQN12_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCS1655121.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(1973..3931)
                     /gene="acs"
                     /locus_tag="OQN12_002872"
     CDS             complement(1973..3931)
                     /gene="acs"
                     /locus_tag="OQN12_002872"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCS1655122.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4324..5760
                     /gene="nrfA"
                     /locus_tag="OQN12_002873"
     CDS             4324..5760
                     /gene="nrfA"
                     /locus_tag="OQN12_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1655123.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5805..6371
                     /gene="nrfB"
                     /locus_tag="OQN12_002874"
     CDS             5805..6371
                     /gene="nrfB"
                     /locus_tag="OQN12_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1655124.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6368..7039
                     /gene="nrfC"
                     /locus_tag="OQN12_002875"
     CDS             6368..7039
                     /gene="nrfC"
                     /locus_tag="OQN12_002875"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1655125.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7036..7992
                     /gene="nrfD"
                     /locus_tag="OQN12_002876"
     CDS             7036..7992
                     /gene="nrfD"
                     /locus_tag="OQN12_002876"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1655126.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8012..9730
                     /locus_tag="OQN12_002877"
     CDS             8012..9730
                     /locus_tag="OQN12_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1655127.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9723..10106
                     /gene="nrfF"
                     /locus_tag="OQN12_002878"
     CDS             9723..10106
                     /gene="nrfF"
                     /locus_tag="OQN12_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1655128.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10103..10699
                     /gene="nrfG"
                     /locus_tag="OQN12_002879"
     CDS             10103..10699
                     /gene="nrfG"
                     /locus_tag="OQN12_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1655129.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11041..12354
                     /gene="gltP"
                     /locus_tag="OQN12_002880"
     CDS             11041..12354
                     /gene="gltP"
                     /locus_tag="OQN12_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1655130.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12432..13121)
                     /locus_tag="OQN12_002881"
     CDS             complement(12432..13121)
                     /locus_tag="OQN12_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1655131.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13215..15362)
                     /gene="fdhF"
                     /locus_tag="OQN12_002882"
                     /pseudo
     CDS             complement(13215..15362)
                     /gene="fdhF"
                     /locus_tag="OQN12_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(15560..17026)
                     /gene="mdtP"
                     /locus_tag="OQN12_002883"
     CDS             complement(15560..17026)
                     /gene="mdtP"
                     /locus_tag="OQN12_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1655132.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17023..19071)
                     /gene="mdtO"
                     /locus_tag="OQN12_002884"
     CDS             complement(17023..19071)
                     /gene="mdtO"
                     /locus_tag="OQN12_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1655133.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            complement(19071..20102)
                     /gene="mdtN"
                     /locus_tag="OQN12_002885"
     CDS             complement(19071..20102)
                     /gene="mdtN"
                     /locus_tag="OQN12_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1655134.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
BASE COUNT         4509 a         5368 c         5541 g         4791 t
ORIGIN      
        1 cgaggatctg tacccaaata gtcgggccga ggatcatcag caccactgcg gtaatcagcc
       61 ccagccagcc gcccagcatc gcgccacggg tagtcagttt cgaccagtac atggaaagca
      121 gaatgatcgg gaagttacag ctcgccgcga tggcaaacgc aaggcctacc ataaaggcga
      181 tgttctgatt ctcaaacagc acgccgagga taatcgcaat cacgccgagg atcagtacgg
      241 tgatttttga tacccgcagc tcttcacgtt cggtcgcgcc ttttttgaag acgttagcgt
      301 acaagtcatg cgaaaccgcc gatgcgcccg ccagtgtcag atccgcaacc accgcgagga
      361 tagtggcgaa agcaaccgct gaaataaaac cgaggaatag gttgccgccc actgcattcg
      421 ccaggtgaac ggccgccatg ttgttaccac caatcagatg gcccgccgcg tctttatatt
      481 ccggattcgc accaacaagc atgatcgcgc cgaagccgat aataaaggtc agaatataga
      541 agtagcccat aaatccggtg gcgtagaaca cgctcttacg tgcttcgcgg gcatcgctga
      601 ctgtaaagaa gcgcatcaga atgtgcggca agcccgccgt accaaacatc agtcccagcc
      661 ccagagagag cgcggagatc ggatctttca ccagtccgcc cggtttcatg atgtcgacac
      721 ctttcgggtg taccgccatc gcttcgctga acagattgtt gaagctaaag ccgacgtgtt
      781 tcatcaccat aaaggccata aagctggcac cgaacagcaa cagcacagct ttgataattt
      841 gtacccaggt ggtcgccagc atgccgccga acaggacgta catcatcatc agcacgccga
      901 ccagcaccac cgcaatgtga tagttaaggc caaacagcag ctcgatcagt ttacctgcgc
      961 ctaccatctg agcgataagg taaagcgcca ccactaccag agaaccacag gccgaaagaa
     1021 tacgaatcgg cccttgtttc agacgataag aggccacatc ggcagaggtg tagcgtccca
     1081 ggttacgcag acgttcggca atcagaaaca gaatgattgg ccagcccacc aggaagccca
     1141 acgagtagat cagcccgtca tagccggagg taaacaccag cgcggagatc cccaagaatg
     1201 aggcggcgga catatagtcc ccggcaatcg ccagcccgtt ctggaagcca gtgatattgc
     1261 cgcctgcggt gtagtagtcg ctacgagaac gtacgcgttt tgatgcccag taggtaatgc
     1321 cgagcgtaaa cacgacgaaa atcaggaaca taataatcgc ctgccagttc gttggctggc
     1381 gctctacggc cccgctaata gcatccgcgg cgttagctgc gaaagggagt gtggcggcaa
     1441 gcgccgtcag aactcttttc atgatgcttg tacctcatgc aggacttcat tattaagacg
     1501 gtcgaattcg ccgttcgccc gccagatgta gataccggtg agaacaaagg agatcacaat
     1561 cactccaaca ccaattggaa taccgcgtgt gacgctggtg ttcggattca gcggcgtgcc
     1621 cagccagccg ggcgcgaagg cgatcagtaa aataaagccg atataaactg ccagcataat
     1681 aatcgacagg atggtggcaa accgttgccg tttttcgact aactccctga aatgcgcatt
     1741 gtcttctatc cgctgataaa tcgtaccatt catcacagat tctccagagg taatgtaggg
     1801 attgttttaa ttcccgctcc cttatgggag aaggttaacg ctcgggtaac ccttgccgaa
     1861 tgtaggccgg ataaggcgtt tacgccgcat ccggcaatca atgcctgatg cgacgctgtc
     1921 gcgtcttatc aggcctacaa accgttaccg attcgcatcc ggcaattgtg ggttacgatg
     1981 gcatcgcgat agcctgcttc tcttcaagca gcttctcgac cacgccagga tcggcaagcg
     2041 tcgaggtatc gcccaggttg ctggtatcgc ccgccgcaat tttgcgcaga atacggcgca
     2101 taattttgcc ggagcgggtt ttaggcaggg agtcggtcca gtgcagcacg tctggcgtcg
     2161 ccagcgggcc aatctcttta cgcacccagt tgcggacttc tgcgtacagt tctggtgacg
     2221 gttcctcccc gtgattaagc gtgacatagg cgtagatcgc ctgaccttta atattgtgcg
     2281 gaatacctac tacggcggct tcggcaatct tcggatgcgc caccagcgcc gactctatct
     2341 ctgccgtccc cagacggtga ccggagacgt tcagcacgtc gtccacacgc ccggtgatcc
     2401 agtaatagcc atcttcatcg cgacgcgcgc cgtcgccgct gaaatacata tttttgaagg
     2461 tggaaaaata ggtctgctca aaacgttcgt gatcgccaaa cagcgtacgc gcctgaccag
     2521 gccaggagtc ggtgatcacc aggctacctt cggtagcccc ttccagcggg ttaccttcgt
     2581 tatcgaccag cgccggttgc acgccgaaga acggacgtgt tgccgaaccg gctttcagct
     2641 cggtagcgcc aggcagcggg gtgatcatga aaccgccggt ttcggtctgc caccaggtat
     2701 cgaccaccgg acatttctcg ttgccgattt ttttccagta ccactcccac gcttccgggt
     2761 taattggctc gcccacggaa ccgagaatgc gtagcgacga acggtcggtg ccttcaatcg
     2821 ctttatcgcc ttccgccatc agcgcgcgga tcgccgtggg tgcggtatag agaatattga
     2881 cctgatgctt gtccaccacc tgcgccatac gggcaggcgt cggccagttc ggtacgcctt
     2941 caaacatcag cgtggtcgcg ccgcaggcca gcgggccgta cagcaaataa ctgtgtccgg
     3001 taacccagcc cacatcggcg gtgcaccagt agatatcacc cggatgataa tcaaagacat
     3061 atttaaaggt cagcgccgcg tacaccagat aaccgccggt agtgtgcagt acgccttttg
     3121 gttttccggt agaaccggag gtatagagaa taaacagcgg atcttcggcg ttcatctctt
     3181 ccgcctggtg ctgatcgctg gcttgctcaa ccaggtcgtg ccaccacagg tcgcgccctt
     3241 cctgccagtc gatgtttccg ccagtacgct tcagtaccac cacatgctct acgctggtga
     3301 cgttcgggtt ttttagtgcg tcatcaacgt ttttcttcag cggaatacta cgcccggcgc
     3361 gcacgccttc gtcggaagtg atcaccagtc gtgagttgga atcaataatg cgcccggcaa
     3421 cggcttccgg cgagaagccg ccgaaaatca ccgaatgcat cgcgccaatg cgggcgcagg
     3481 ccagcatcgc aaccgcggct tccggcacca tcggcatata aatcgccacc acatcacctt
     3541 ttttaatgcc cagctcgagc agggtattgg cgaagcggca gacgtcgcgg tgcagctctt
     3601 tatagctgat atgtttgctc tggctggcgt cgtcgccttc ccagatgatg gcggtgcgat
     3661 cgccgttttc ttgcagatgg cggtcaaggc agtttgccgc cagattcagc gtgccgtcct
     3721 cgtaccattt aatggacaca ttaccggggg caaaggaggt atttttcacc ttctggtacg
     3781 gtttgatcca gtcgagaatt tttccctgtt cgccccagaa ggtatcaggt acgttaatag
     3841 attgttgata catcgcctcg tactgctgag ggtttatcag gcaacggtct gcgatgttgg
     3901 caggaatggt gtgtttgtga atttggctca tgcttttgtt ctccttgtag gatgttaata
     3961 atatgtggca taagcgttaa atgtaggggt attggcagtt ttgtttagta tttgggcgac
     4021 agatcacgca aaagtagaat ggtgcaaata aacggcaggg taatttttga aggtcaccaa
     4081 caaaagttga ttaattcttt gaggaacatg cagttatgca tgctgttgaa aaagaggaag
     4141 atactgacta actctaaagt ggtatttcac atgcacttac aattgattaa agacaacatt
     4201 cacagtgtgg ttatttgtta cacatagggg tgagcaatgt catgacagtg taggtgcggt
     4261 tactgtcgtg aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc
     4321 cctatgacaa ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc
     4381 tttttcactt ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt aactgtggaa
     4441 gcgaagaatg aaacctttgc cccgcagcat cacgatcaat atctctcctg gaaagccacc
     4501 tcggaacagt cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg
     4561 gcggggtatc ccttctcgcg cgattacaac aagcctcgtg gacatgcctt tgctgtgacc
     4621 gatgtgcgtg aaaccctgcg taccggtgcg ccgaaaaacg ctgaagatgg tccgctaccg
     4681 atggcgtgct ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa
     4741 gatggctact tccacggtaa atgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt
     4801 tgtgccgact gccataacac cgcctcacca gagttcgcca aaggcaaacc ggagttaacc
     4861 ctttcccgtc cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc
     4921 ggacgtttcg accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac
     4981 ggcaaaaaca aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag
     5041 cagtattacg acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg
     5101 ctgaaagcgc agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac
     5161 gtgacctgta tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc
     5221 gaccataaaa ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc
     5281 caggataaag ctgccttgca aaaagtggtc gcggaacgta agcaatcgat taacgacctg
     5341 aaaatcaagg ttgaagatca actggttcac gctcacttcg aagcgaaagc ggcgctggat
     5401 gcaggcgcga cggaagccga aatgaagccg attcaggacg atatccgtca tgcccagtgg
     5461 cgttgggatc tggcgatcgc ttcccacggt attcatatgc acgcaccgga agaaggtcta
     5521 cggatgctcg gtacggcgat ggataaagcg gcggatgcgc gcaccaaact ggcgcgcctg
     5581 ctggcgacca aaggtatcac ccatgaaatc cagatcccgg atatctcgac caaagagaaa
     5641 gcccagcagg ccattggcct gaacatggaa caaatcaagg ccgagaagca ggacttcatc
     5701 aaaacggtga tcccgcagtg ggaagagcag gcacgtaaaa acggtctgtt aagccaataa
     5761 ccccgttccg cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt
     5821 cgttattaac tgctggggtg ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg
     5881 ctacccctgc ggtgcaggca tctggtgatc gctacgaagt tacccagcag cgtaacccgg
     5941 atgccgcctg tctggactgt cataaaccag ataccgaagg tatgcatggc aaacatgctt
     6001 ccgttatcaa cccgaataac aaattgccgg tcacctgcac caactgtcac ggccaagcat
     6061 caccgcaaca ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtataagg
     6121 tcggagagca gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg caaaaagcgt
     6181 tctggccgca cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc
     6241 cacagcaaga tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt
     6301 gccacagcga tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg
     6361 agcagccatg acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt
     6421 cagcggcacc gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg
     6481 catggtgcat gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcggga
     6541 agtgaacaag gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg
     6601 cgaatttcct gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgt
     6661 gccgtgcgtt gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt
     6721 cgatgttaat cctgatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg
     6781 cgtgcgcttt atccatccgg tcacgaagac ggcggacaaa tgcgatttct gtcgtaagac
     6841 caatttgcag gccggtaagc ttcccgcgtg cgttgaagct tgcccgacga aggcgctgac
     6901 gtttggcaat ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac
     6961 ttaccgctac aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata
     7021 cggggaggtg agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac
     7081 tggccgattg ccatctacct gtttttgatt ggtatttctg ccggtctggt gacgctggcc
     7141 gtgctgttac gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt gctgcgcacc
     7201 acgctgattg tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg
     7261 acaagaccgt ggaccttctg gaagctgatg ttccactaca gttttacctc ggtgatgtcg
     7321 atgggggtga tgctgtttca gctctacatg gtggtgctgg tgctgtggct ggcgaaaatc
     7381 tttgaacatg atttgcttgc cctgcaacaa cgctggttgc cgaagctggg gatcgtgcaa
     7441 aaggttctga gcctgctgac gcccgttcat cgcggactgg aaacgctgat gctggtgttg
     7501 gcggtgctgc tgggggctta taccggcttt ctgctgtcgg cgctgaaatc gtatccgttc
     7561 ctcaataacc cgagcctgcc ggtgctgctc ctcttctccg gcatctcgtc cggtgcggcg
     7621 gtggcgctga tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcac
     7681 tttgttcacc ggatggaaat ccccgtggta tggggtgaaa tcttcctgct ggtggcgttt
     7741 tttgtcggtc tggcgctggg cgatgacggt aaagttcgtg cgctggtggc ggcattaggt
     7801 ggcggtttct ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca
     7861 atgttgctca aaccgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt
     7921 ggggccagtc tggtcggcgt gttgatgctg cgctttttca ttctctacgc cggacagttg
     7981 acggtggcgt aagccagaaa agaggtggtt tctggacgta ttccttcctg aagtcggttt
     8041 tctggcgttg ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg
     8101 agtgcggttg cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcgcagtt
     8161 cgcgctcctg ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc
     8221 ggtcatttat gtcgcccaac atagctacag cctgctgtcg tgggaactca aactggcggc
     8281 ggtgtggggc ggtcatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag
     8341 cgcgctgttt gcctggcatt atcggcagca aagcgatccg ctatttccgc tgacgctagc
     8401 cgttttatct ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt ccgatccctt
     8461 cgtgcggata tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc
     8521 cggtcttatc tttcatccac cgctgctttg ccttggctat ggcggtttga tggtagcggc
     8581 gagcgtggcg ctggcgagtc tactgcgcgg cgagtttgat gcggccagcg cccgaatttg
     8641 ctggcgctgg gcgttacctg gctggagtgc attaacggcg gggatcatcc tcggttcctg
     8701 gtgggcctac tgcgaactcg gctggggcgg ctggtggttc tgggatccgg tggaaaacgc
     8761 ctctttatta ccctggcttt ctgccactgc gctgctgcac agtttgtccc tgacacgcca
     8821 gcgggggatt ttccgccact ggtcgctgtt gctggcgata gttactctga tgctgtcgct
     8881 gctgggcacc ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgcactgga
     8941 taacgtccgc gccgtgccgt tgttcagcct gtttgcactg attagccttg cgtctttggc
     9001 tctgtatggc tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg
     9061 ggaaatgtta atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt
     9121 gggaacgctt tatccgatga tttacggcct gctgggctgg ggacgcctct ccgttggcgc
     9181 gccgtatttt aaccgcgcga cgttaccgtt tggtctgttg atgctggtgg tgattgtgct
     9241 ggcgacgttt gtctctggca aacgcgtgca gcttccggcg ctggtagctc atgctggcgt
     9301 gctgttattt gccgcgggga tcgtggtttc cagtgtcagt cgtcaggaga tcagcctgaa
     9361 tttacagccg ggtcagcagg tgacgctggc aggatacacc ttccgttttg agcgactcga
     9421 tctgcaagcc agaggcaatt acaccagcga aaaagcgata gtggcgctgt ttgaccatca
     9481 gcaacgcatt ggtgaattaa cgccggagcg gcgtttttat gaagcacgcc gtcagcaaat
     9541 gatggaaccg tcaattcgct ggaacggcat ccatgactgg tatgcggtca tgggtgaaaa
     9601 aaccagagcg gatcgttacg cttttcgttt gtatgtacaa agcggtgtgc gctggatctg
     9661 ggggggagga ttgttgatga ttgcgggcgc attgttaagc ggatggcggg ggaagaagcg
     9721 cgatgaataa agggcttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg
     9781 ttgtagacac ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca
     9841 gccagttacg ttgtccgcag tgccagaatc aaaacttact cgaatccaat gcgccggtgg
     9901 cggtcagtat gcgccatcag gtttatagca tggtgtcgga ggggaagaac gaagtcgaaa
     9961 tcattggctg gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg
    10021 gtcagacgct ggtgttgtgg gcgctgccag tggtgttgtt attgctgatg gcactgatcc
    10081 tctggcgagt gagggctaag cgatgaaaca gcctcaaata ccggtgaaaa tgctgacaac
    10141 actcacgata ttgatggtat tcctctgtgt cggcagttat ctgttaagcc caaaatggca
    10201 ggcggtacgt gcggagtatc agcgtcagcg cgatccgcta catcagtttg ccagccagca
    10261 aaccccggaa gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag
    10321 cgaacaatgg gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct
    10381 gctggcgtat cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgcggcgct
    10441 ggcgacggtg ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat
    10501 cgacaaagcc ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga
    10561 tgcgtttatg caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct
    10621 caactcaccc cggatcaacc ggacacagct ggtcgagtcg attaatatgg caaaactgtt
    10681 gcagcggaga tcggattaat catctggtga tgaagattat gtcattgagc tgcataaaaa
    10741 ataatcgaat gaacatatgc caaaaataat caccaatcag tattattgca gattaaacaa
    10801 ataaaaatct ttccataata aatggttatt cattaatcct gccataaact cgcgttatta
    10861 tgcattaatg cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga
    10921 tactgatcat aagcgttaaa aaaatctaca aaccaacgca acacaattca tgccctggca
    10981 gtatgtcacg ttctcgcgtt tctgaacggg gaacggcgct ccattgagga agttattctg
    11041 atgaaaaata taaaattcag cctggcctgg cagattctgt ttgctatggt gctgggcatt
    11101 ctcctgggaa gctacctgca ctaccatagc gacagccgcg actggctggt cgtcaatttg
    11161 ctctctccgg cgggtgatat cttcatccat ctgatcaaaa tgattgttgt gccgattgtg
    11221 atctccacgc tggtggtggg tatcgcgggt gttggtgatg ccaaacagct cgggcgtatt
    11281 ggcgcgaaaa ccattattta cttcgaggtg atcaccaccg tcgccatcat tttggggatc
    11341 actctggcga acgtcttcca gcccggtgcc ggggtggata tgtcgcagct ggcgaccgtc
    11401 gatatctcga aatatcagag cactacggaa gcggtacaaa gcagttccca cggcattatg
    11461 ggcacgattt tgtcgctggt gccgacgaac attgtggcgt cgatggcgaa aggcgaaatg
    11521 ctgccgatca tctttttctc ggtgctgttt ggtctggggc tttcttccct gcccgcgacg
    11581 catcgtgaac cgctggtgac cgtgttccgc tccatctctg aaaccatgtt taaagtgact
    11641 cacatggtga tgcgttatgc accggtgggt gtgtttgcgc tgattgcggt gacggtggct
    11701 aactttggtt tctcgtctct gtggccactg gcgaaactgg tgctgctggt gcatttcgcc
    11761 attctgttct tcgcgctggt agtgctggga attgtggcac gtctgtgcgg gttaagcgtc
    11821 tggatcctga ttcgtattct gaaagatgag ctgattctgg cgtactccac tgccagctct
    11881 gaaagcgtac tgccgcgaat tattgagaag atggaagcct acggagcacc ggcgtcgatc
    11941 accagcttcg tggtgccgac cggttactct tttaaccttg atggttcgac gctgtatcaa
    12001 agtattgccg ctattttcat cgctcagctg tatggcattg acctgtccat ctggcaggaa
    12061 atcattctgg tgctgacgct gatggtgacc tcgaaaggga ttgctggcgt ggctggcgtg
    12121 tcgtttgtgg tgttgctggc aacgctgggt agcgtaggta ttccgctgga aggtctggcg
    12181 tttattgctg gtgttgaccg tatcctcgac atggcgcgta ctgcgctgaa cgtggtgggt
    12241 aatgcgctgg cggtgctggt gattgccaag tgggaacaca aatttgaccg caaaaaggcg
    12301 ctggcttatg agcgtgaagt gctgggcaaa tttgataaaa ctgcggatca ataattgaag
    12361 attgccgggg atatcccccg gcaatgtgtg gatgcctgat gcgacgcttg ccgcgtctta
    12421 tcaggcctac cttaaccgtt ggttaatttt tcaaactctt cacacccggt atcaaaccct
    12481 tccatacagc tcaggttcaa ccagtgcagc gccttttgtt tgttcttctc gataaaacct
    12541 tcctcaccgt ttaagaacat cattcccgcc cagtactcgg aataaccggt tcgggaaatt
    12601 gcagagctgc gtttgaaata ccaggttgcc ttgtcatcgt cggccttaat gcccacgccg
    12661 ttggcgtaaa tcaaaccaag cagcatttgg gcatcgacgg cggagtcatt ctccagatct
    12721 tccgaggcgt tttctaacag cgaaatcgcc tttggataat ccggtttacc cgcttgagta
    12781 tttaccagaa tatgcgccag cgttacttca ccttctttac tgcccgcttg cgccgctttc
    12841 tctgcaagaa cttttgcttg tggatagtcc agactgaccg gattggtgat tttgatctgc
    12901 gccaacagcg cgcaggcatc agcatcaccg ctgtcggcgg ctttctgtgc ccaatattcg
    12961 gctttgctca aatcgccgga gctaaaccag ctgtcggcaa gaaaatattg tgcacgtcgg
    13021 tccccggcct ctgctgcctt taaatactgg ctgccaggct cggagtcgtt cgcgtgggca
    13081 aagaatgtca aaaacaacat caatgcgata atttttttca ttttgataat acgattgggt
    13141 agggagtaac cagtataaag agttcgaatg gataaaaaaa cagcctccga aaggaggctg
    13201 tagaaaggac tgtattacgc cagtgccgct tcgcgcaggc gagttttcaa cttgttgtac
    13261 tcgtcaatca cgtactgctc ggcggcgcgc tgatcggaga tcggctcgac gcgcacggcg
    13321 cagtatttgt attccggcgt tttcgtaatc gggcttaagc tttcggtaac cagctcgtta
    13381 caggcaccaa tccaccactg gtaggtcatg taaatcgccc ctttgttcgg acgatcgctg
    13441 acctgcgcac gggtgataat tttgccttta cgcgagtgca ctcaaaccaa tgcctcatct
    13501 tcaataccca gacgtttggc gtcttcggta ttgatttgtg cgtagccagg ttcatcagcc
    13561 agcgccgcca gtgccgcaca gttaccggtc atcgaacggc aagagtagtg accaacttca
    13621 cgcaccgttg acagtaccat cgggtactcg tcggtgagtt tgtcgattgg cgctacccag
    13681 tcgcaggtga agaactgcgc cagaccgttc ggggtatcaa acttctcttt aaacagataa
    13741 gaagtcccct gatcggcatc tgaagtatcg cggcaaggcc actgaatgaa gcccagttcg
    13801 cccattttct cgtaagtcgc accgtagaaa tccgggcaca gatgacgcaa ctcatcccag
    13861 atctcctggg tgttgttgta gtgcatcgga taacccatac gggtggcgat ttcactgatg
    13921 atttgccagt ccgttttcag atcccatttc ggttcaactg ctttgaagaa acgctggaag
    13981 ccacggtcag ccgcagtaaa cacgccttca tgctcgcccc acgacgttga cggcaaaatg
    14041 acatccgccg ccgacgcggt tttggttata aagatgtcct gaacgatgac cagttccaga
    14101 tcttcaaagg ctttacgtac tgccgacagc tccgcatcag tttgcagcgg atcttcgccc
    14161 ataatgtacg cggcacgcac ttcgccatgc gctgcgcggt gcggcagctc gctgatgcga
    14221 tagccggtat gagcaggcag gctttccacg ccccaggctt tggcgaattt ttcgcggtta
    14281 gccggatctt tcacgtactg gtatcccgga tacgtatccg gcagcgcgcc catatcgcag
    14341 gcaccctgca cgttgttctg accacgaacc gggtttacac ccgcatgcgg cttaccgagg
    14401 ttactggtca gcatcgcgag gctggtcaga gaacgcacgg tttccacgcc ctggtagaac
    14461 tgggttacac ccatgcccca caggatggcg gcgcttttcg cctgggcata catccgtgcc
    14521 gcctgacgaa tctcactggc gctgacaccg gtgatatctt caaccgactc cggcgtgtag
    14581 ccttcaacga ttttacgata ctcttcaaag ccttctgtac gggaagcgac gaacgctttg
    14641 tcgtacagat tttcttcaat aatgacatgg cccatcgcat tcaacagcgc gatgttcgag
    14701 ccgtttttca gtgcaatgtg catgtcagca atgcgcgcgg tttcaatttt gcgcggatcg
    14761 cagacgataa ttttcgcccc gttacgttta gcgttaatta cgtgattcgc cacgattggg
    14821 tgggaatccg ccgggttgta cccgaaaacg aacactaaat cggtattatc aatttcgtta
    14881 atagcattgc tcattgcgcc attaccgacc gattggtgca gacctgcaac cgatgggccg
    14941 tgtcagacac gagcgcagca gtcaacgtta ttggtaccaa taacggcgcg cgcaaatttt
    15001 tgcattacat agttggtttc gttacccgta ccacgcgagg agccggtcgt ctggatggca
    15061 tccggaccgt acttctcttt gatggcgctc aggcgctcgg caacgtaatt cagtgcctca
    15121 tctcaggaaa caggttcgag tttgccgcca cgctggcgac ggatcatggg ggttttcagg
    15181 cgcggggtca ggatctgggt atcgttaatg aaatcccagc cataataacc cttcagacac
    15241 agggtaccct ggttggtttt cccctgcgct gcctccgccc ggacgatttt gccgttatcg
    15301 acgaccaggt tgattttgca acctgatgcg caataggggc aaaccgtgac gacttttttc
    15361 atcggtctcg ctccagttaa tcaaatcacg catacgcgct ctcgactaca gtatgcatct
    15421 tttatgccac attttatttg gggtcattcc ctgatattac gggcactatt tattcaaaac
    15481 tctgacgaaa aacaggctgt cgtcagtttt gacgtgacga aacgaaatac cgcgtgacag
    15541 ccatcacgcg gcagacattt tattttttct cgacgacggg agccgcctga tacccgccgc
    15601 ccagcgattt catcaactga atgctttgga tcacccggcg gctgtccagc atcagtaatg
    15661 acatctcttc ggcaagtact ggcaaccggg cttcagtggc ctgtaagcgg ctggttaagc
    15721 cgcgctgata ggcggcctcg gcagcgcgct gggtgaagcg cgttgcttcc acgtgttcag
    15781 cctgcatttc tcgctcgtcg ttgagcgttt gcagacgcgt gccgttgacg gcaacgtcac
    15841 gcaccgcgtt cagtactgac tggttgtaac gttcaatcat catgttgctg gcggcgcgcg
    15901 tgccttcgag attggcattc aaccgtccac cgtcaaacag cggcaatttc agacccggga
    15961 tgaagttgaa ctggcgactg gtttttttga ataaggtatc caggtggatg gcatcaaggc
    16021 cgaaaaacgc tttgatatca aagctcggat agaacaacgc ccgcgcggaa tccacctgat
    16081 ctaatgacgc ctgaacatac cagcgcatgg cttgcagatc cgggcgtctg gcgagcaact
    16141 cataagagag tgtcgccgga atgccggtct ggactcgtgg taatgccaca ggtttgatct
    16201 ccggcatatc gctggctccc gcgccaatca atgcgcgcag agattctctc gtttcggtga
    16261 tttgcccttt gacggcagca atttgtttat cgaccgccag aatctgtgcc cgcgcgccgt
    16321 ggaaaggcac ttgtgcttcc agaccgtgcg ccactttact ttggtgcgct ttcaccgcgt
    16381 aatcaatcac atcgcgagtt tgttctaaca gatcgagcat ctgatagctg gcctgcatac
    16441 tgtaataaag ctgcgctacg cccgtggtca gcgatagctc tactgctgcg gtttctgcca
    16501 gcgcggcatt atgcgcgcca atggcggcgg caaccgctga gcgatgcaca ccccacaaat
    16561 caagatccag tccggcaaac aaacctactg tggcttccgt atagtacggc ccgtccatcc
    16621 ccagtgcggg cgcatccatc gcataagggc ttaaaaagcc gttcgccgag acacgttggc
    16681 ggttaagcat ccctaacgct gccacctgca actgtgaacc ggcatctaac aaatcggcct
    16741 gcgattgcgc tttttcttcc cgcagtttcg cttcggcgag ggtgtgtgaa ccacttagcg
    16801 tccgttggat cagggcatcc aactgcgggt cattgagttg tttccaccac tgcgcctgcg
    16861 gccagccgga gctggcaaga tgaatatcat cggccagttt gatttgttcc ggtttgagct
    16921 gttgatgagg cgcagaatcc ttacgtacca gggcacagcc ggaaatcagc gtcgtgctgc
    16981 cgagaatgct gcacaacagc agacgtgaaa gttgacgatt gatcattgcg tggctccttg
    17041 tgcctgttcc gtggcgggcg ttaatgccgg ggctgtccag tctggcaagc gggcgaaaag
    17101 ctggcagacg tgttgctcct gttttaataa ggtgggcagg atggcctgcg aggtgggtgt
    17161 ctcttcaagc gttatttgag gagaacggct gagtgcggtt gccagaccgg cagcgtattt
    17221 ctccagggcg tcggcaaact gacctgctct gtccggctgt agtgcgttat ccagcgcctg
    17281 cgccgaattc caggtggcat cccaggcgtg aaggatatcg cggccctgac gaataaccgt
    17341 ttgcgaacgt tcaatcagta aggcgcgttc ttcgctgtcc agttgacgct ccagcgccac
    17401 gcgttggcac atctcttcac aggcattaaa cgccgcatgc agaccgatac gaatttgcag
    17461 ataagtgcgc agagccgtga cttcctgctg gcgtgggatc cgcattactt tacttaacat
    17521 acccagcgcg ccagccagtt tttgcggcag cgtgcgcgct tcactttcag gccagacaaa
    17581 ggtgtaaatc atcgcggaca ccacggtacc aatgatgata cccagggcgc gatcgcgaat
    17641 ttccaccagg tcgtacactg ggccaaaaac gttttcgagc gtggcgagcg cgaaggtgac
    17701 caccatctgt gtgccgatat aagaagagcg ttcagagctg gtggcaatcc atgcggccaa
    17761 caggaaaatc ggtgccagca caaacagcaa ttcgacaata ttgtccagcc agggcatgac
    17821 cagtagcgtg aataacagcg ccagaatcgc gccgcaaaag gccccgccaa aacgcagcac
    17881 catcttctgg tacgacgaac cgacatttgg attagcgacg atcacgcagg tcagcataca
    17941 ggtgtgaatg ccttcccagt ccacgccgct gtaaaaggtg taacagatca aacaggcgag
    18001 caatgttttt accgcgtagc gtatatagtc tggattggta aaagcatcgg cgaccattga
    18061 tggcggtttg gcggcgggcg ttggcggcgt attcgggttc atctggccca gttgtaacaa
    18121 cgtctggcag atattctcca ggttacattc ccgtgccgcc accgcttcac tttcactgat
    18181 ccgccagtcg ctttgccagc actgaccttc agcaacggca tgctgcagct tgttgatttc
    18241 tgaagctaat ttttgccgaa attcaataat tgcctgagaa tcagcaaaag aggtgggatc
    18301 gtagcgattc agcgtcgagt aaatgtaggt taccgttgcc acgcagcttt gccaccatgc
    18361 gctttgagtt cgccagttgg catcgtccgc gagacaaaag acattcagtt tttgcagcgc
    18421 cagcgcctct ctttcaatcc gcgtttcggg tagcggtgcg aggctgtccg tcaggtggct
    18481 aatggcatca tcaagccgat cattaagcgc ttgatgcatt tgcgtaatgg cacgattggg
    18541 aaaccacagc acgccgatta acgtcatcag caaggttgga tagaggccaa caacgataca
    18601 ccacagcgtt aagcgcacga ctacttccgg atagtcgagc atggcgggga aggtttgccc
    18661 gtaaatggcg acagcgaaaa agaccagtcc cagccgatgg gtgcgcatca aaaacatgca
    18721 gcccatcagg atcggcccgg cgatgatcaa tcggatcaac ggttcgccgt atgaccattt
    18781 gtagatcaaa aacaggctgc cgatctccag caccgtggca accacaaaca agatcgccac
    18841 aaatttggtg taaaacgcgt tcgactgaat accgataccg taaaacagca ctgccagcga
    18901 taacgccaca aaagggatct caaaagtcat cgagatcaga atcaccagca ggcagcctac
    18961 ccagagttgc acgatctgcg gcacgcggcc gggtcgccgc tcgcttaact cttcatgaaa
    19021 gaacgccagc agcctgacca ccggtaatgg cagggagttg agcgcgctca ttattgcggc
    19081 tcaagattag cgactgccga agcgccgatg cggaacattt ccgggtcagg tttatcgacc
    19141 atgattttga ccggaaaacg ctgggcaacg cggacccagt taatagaacg agacactttt
    19201 ggcaggccgc ccagcaccag gccgccgtca tccggtagca cgccgtagcc aatcgaatcc
    19261 actttaccct cgaaggtttt gccgctgtca ctcatcaggc gaatcgttgc gggtgtacct
    19321 gagcgaatat ttttcagatc ggtttcgcgg aagttggcga tcacgtacca gtgacgagtg
    19381 tcgatgaggg taaaaatagg gcgcatggca gaagcaaatt gcccgacgga ggttttgagg
    19441 gaaatgatcc ggccatcaaa tggcgcgcga acggtcgtca tttccagatg tagtttggtc
    19501 agggcaatat ccgcttcgac cgccgcacgt tgcgccacca gcgcatcgac gccgctgacg
    19561 gcgctggcgg ctgactgcgc ctgtaacaat acggcattaa gatccgcttc tgcggcgcgc
    19621 tgcgccgttc ttgcacggtc aacgtcttct gctgagacaa aaccctcttt cagtaatggc
    19681 tcggtgcggc gtaatgtatc tgtggcctgt ttcgcggcgg cacgggcttt ttctaccgtg
    19741 gcattaaccg agtcggcacc aaactgttgg gcgtcaacgc tacgctgggt gagcataatt
    19801 tgcttatcca gcgccgcgag ggaggcttcg gctttcgcca ggttggcttc gtacgggcgc
    19861 gggtcgatgc ggaacagcaa atcgccctgt ttcaccgcct ggttgtcggt gaccgccagt
    19921 tctacaatgc gaccgctgac ttccggcacc acatcaatgg tatctgctga cgcgtaagcg
    19981 tcattagttg atggcgcact gtctacgagc cagataacga aaacaagggc aaccagcgcc
    20041 aacgcaacca ctaacagagc agggaattta ctgcgaggtg cttttttcgg cgtactttcc
    20101 atgagcgcgt ctcaaataga ttagaaaaat gccagccaga acagcatggc gtagagagca
    20161 aaaagggcgg tataaataat tccgacaaat gccagattga tatttgccc
//
LOCUS       DAJVRP010000081        19927 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVRP010000081 DAJVRP010000000
VERSION     DAJVRP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.081"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..304
                     /locus_tag="OQN12_002886"
     CDS             <1..304
                     /locus_tag="OQN12_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCS1655135.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            276..2204
                     /locus_tag="OQN12_002887"
     CDS             276..2204
                     /locus_tag="OQN12_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCS1655136.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2188..2391
                     /locus_tag="OQN12_002888"
     CDS             2188..2391
                     /locus_tag="OQN12_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1655137.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="OQN12_002889"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="OQN12_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="OQN12_002890"
     CDS             3971..5476
                     /locus_tag="OQN12_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCS1655138.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="OQN12_002891"
     CDS             5513..5860
                     /locus_tag="OQN12_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1655139.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="OQN12_002892"
     CDS             5918..6946
                     /locus_tag="OQN12_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1655140.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /locus_tag="OQN12_002893"
     CDS             6998..7384
                     /locus_tag="OQN12_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1655141.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="OQN12_002894"
     CDS             7396..7773
                     /locus_tag="OQN12_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1655142.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..8344
                     /locus_tag="OQN12_002895"
     CDS             7760..8344
                     /locus_tag="OQN12_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1655143.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8341..8742
                     /locus_tag="OQN12_002896"
     CDS             8341..8742
                     /locus_tag="OQN12_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1655144.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8753..9493
                     /locus_tag="OQN12_002897"
     CDS             8753..9493
                     /locus_tag="OQN12_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1655145.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            9552..9938
                     /locus_tag="OQN12_002898"
     CDS             9552..9938
                     /locus_tag="OQN12_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1655146.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            9947..10276
                     /locus_tag="OQN12_002899"
     CDS             9947..10276
                     /locus_tag="OQN12_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS1655147.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10248..12986
                     /locus_tag="OQN12_002900"
     CDS             10248..12986
                     /locus_tag="OQN12_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1655148.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12986..13315
                     /locus_tag="OQN12_002901"
     CDS             12986..13315
                     /locus_tag="OQN12_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1655149.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            13315..14013
                     /locus_tag="OQN12_002902"
     CDS             13315..14013
                     /locus_tag="OQN12_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1655150.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            14018..14761
                     /locus_tag="OQN12_002903"
     CDS             14018..14761
                     /locus_tag="OQN12_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1655151.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            14698..15300
                     /locus_tag="OQN12_002904"
     CDS             14698..15300
                     /locus_tag="OQN12_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1655152.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15361..18841
                     /locus_tag="OQN12_002905"
                     /pseudo
     CDS             15361..18841
                     /locus_tag="OQN12_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            18909..19508
                     /locus_tag="OQN12_002906"
     CDS             18909..19508
                     /locus_tag="OQN12_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1655153.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19530..>19927
                     /locus_tag="OQN12_002907"
     CDS             19530..>19927
                     /locus_tag="OQN12_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1655154.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT         4644 a         4699 c         6288 g         4296 t
ORIGIN      
        1 actcattgcc agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca
       61 ggtgtcaaaa ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc
      121 gatggcttta tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga
      181 tttaacgccg cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg
      241 cgcgcgtgcg ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga
      301 gtaatatgat ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga
      361 cggctgtaga atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg
      421 gggaatggaa aacgctgccg tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga
      481 ttcgtacagt taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg
      541 tgatcgggta ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact
      601 cagctgctga agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc
      661 tgaaaaaact ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc
      721 gtttttcctc tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg
      781 aaaaatctgt cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa
      841 aagaaggttc gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat
      901 ccatacgcgg ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg
      961 aatctgcgca tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc
     1021 tgaagtttgg cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa
     1081 cggtgtatta cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga
     1141 ccggggggcg ctggatttgt gacaataccg ggatgtggac gcgtgacggt ctgacgtttt
     1201 tcagtgcttc gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg
     1261 cgtacagtcc gttcaccacc tgggtacaga tagtctgtga ctggctggat gcactgaaag
     1321 atcccaacgg cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg
     1381 tgggcgaaaa actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg
     1441 tgcctgtccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga
     1501 tgtatgtctg ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca
     1561 tgggacgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat
     1621 accgccatgc agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga
     1681 tcgatggcga aatcgtgtat cagaggtcaa aaaaacacgg tgttttccgg gtactgccgg
     1741 taaaaggtgc ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc
     1801 ggggtgtgta tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa
     1861 tgaaggccga tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata
     1921 atccggagat tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa
     1981 agtgggaaaa aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc
     2041 tggactgcct ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg
     2101 atctggctgt gctggcggca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ccaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgtgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 ggctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagtcctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggcagcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacgacccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattgatg tggtgaaaat
     8221 cccgatgtcc ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc
     8281 ggaaatgccg aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa
     8341 atgaaccgac acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga
     8401 gacagcgcca cgttttttga cgggcttccg gcatttattg atgcgcagga actgcctgcc
     8461 gtggcggtgt ggctgagtga tgctcagtac accggaaaaa tgacggatga agatgactgg
     8521 caggctgttc tgcatattgc cgtcttcatc cgggcacagg caccggattc agagctggat
     8581 atgtggatgg agagcaccat tttcccggcc ctgaatgatg taccggcact ttccggactc
     8641 atcgacaccc tgaacccact cggttttaac tatcaacgtg ataatgagat ggctacctgg
     8701 gcgatggcgg aaatcacgta ccagatcacg tacacgaatt aaggaggtgg taatgaccac
     8761 accaaatcca ctggcaaaga cgaaaggtgc ggggacgacg ttctggatgt ataccggcaa
     8821 cggcgatgcg tttgcgaacc ctttgtcgga cactgactgg ctgcgtcttg cgatggtgaa
     8881 ggatctgcaa cctggcgaaa tgaccgctga tgcagaagat gacacttatc tcgatgatga
     8941 agatgcagac tggaaaacga caacccaggg gcagaaatcc gttggtgata cttcggcgac
     9001 gctggcctgg cgtccgggtg acagcgggca gaaaaaactg gttcagttgt tcgactccgg
     9061 tgaagtctgc gcgtttcgta tcaaatatcc caacggcact gttgatgttt tccgcggctg
     9121 gctgagctca ctgggtaaaa ccattgcctc aaaagacgtg atgacccgca ctgtgaaaat
     9181 cagcggtgtg gggcgtccgt atctggcaga ggaaggcact gaaaccgtgg gtgttaccgg
     9241 actgacggtg gcaccggcat ctgtcagtgt caaagcggga gcaaccacca cactgacctt
     9301 tacagtaaaa cctgacgggg ccagtgacaa agcgatcagt gtgcattcgt cagatcctca
     9361 gactgcttcg gtgaccctga gcgggcttgt ggccacggtg aaaggcgtga agcagggcag
     9421 tgtcagcatc gtgggcatga cctctgacgg agagtttgtg gcagtggctg cggtgaccgt
     9481 cagcgcacca taacaggacg atactcatca ttgccccggt tatccggggc tttttgcatc
     9541 cggagaacat gatgtttctg aaacaggata cgtttaatta tgaaaaacag tccgtggtgc
     9601 tcagtgagct gtccgggctg cagagaattg aatatctgac gtttgttcag cagcgaacgg
     9661 caaagtttga tgcacaggag ggagaactgc cggaggctga acgacagatt gcttttctgc
     9721 gtatgggaat ggatatcaat gcctggctgg tttcccgctc actgtggaat gctgagcagt
     9781 ctcaggatgt tgagacgctt tgcgcatcca ttatgacaac atggtcgtat gatgcgctgg
     9841 gcgcgggggc ggagagggtt ctgtcgctga gcggtatggg gaccattgag aatgccgggg
     9901 atgatgatca tgaggcgctg acgccggaaa agtcctgacg cgggaaatgc agtttgtcat
     9961 gcggcttgcc cgggagttcc ggcgggcaga ctggcggcgg atgctgtcgg atatgtcggc
    10021 cactgagctt ggtgagtggg gcgattattt ccggatgcag agcttcagtg atgtgtggat
    10081 ggatgcgcag tttgcctcgc tgaaggcatt gatcgtgaga atggtgtccg gcagcagcga
    10141 tgctgcggtg gctgatttca gccttttacc ggaagagaac gggataccgg agcgaacgga
    10201 cgaagaactg atgcatcttg gggaaggtat ttccggaggt gtgcgttatg gaccagatag
    10261 ccaacctggt cattgatttg gggattgatg cggcagagtt taaaaatgaa attccccgta
    10321 tcaaaaacct tctgaatggt gcagccagcg atgcagaacg gtcttctgcc cgtatgcagc
    10381 gttttatgga gcgtcagact caggcggccc ggcagacaat gcaggcggct tcttcggctg
    10441 caacagcagc atcagcccat gcgcagacgg tggagaagaa tgcacgggct catgaacgca
    10501 tggcccgtga ggtggaacaa acccgtctgc gcgtggatgc cctgaatcag aaaatgcgcg
    10561 aggaacaggc gcaggccagg gcggctcagc tgggggtaag cagtgctgca gaagtgtata
    10621 tccgcaaaat ggagcgggca ggaaaagcca cacattcgct ggggctgaaa agtgcggcag
    10681 cccggcggga gctgggggtg ttaatcagtc agatggcgcg cggcaatttt ggtgcgctga
    10741 ggggatccgg gataacgctg gctaaccgtg ccggatggac aggcgcactg atgtcgccga
    10801 aaggcatgat gactggcggc gttattggcg gacttgtcgc ggcggtcctg ggtctgggta
    10861 aagcctggca tgacggccgg aaggagggcg agaaatttaa ccgtcagctg gcgctgacgg
    10921 gacattatgc cggtgtcact gtcgggcagt tgtggaaact cagccgggcc atatccggga
    10981 atggtatcac gcaacatgcg gcagccggtg cgctggcaca ggtggtgggc agtggggcat
    11041 ttcatggaaa cgatattggt atggtggcga aagctgccgc acagatggag cgatcggttg
    11101 gccagtcggt cagcgatacc ataaatcagt ttaagcggct gaaggatgat cctgtaaatg
    11161 ccgcgaaggc gctggacaat gcgctgcatt ttttgaatgc cactcagctt gagcagatac
    11221 gcgtccttgg ggagcagggg cggtccagtg atgcggcccg gatcgccatg tctgcgctgg
    11281 cagaggaaac cggtaaacgc acatctgata ttgataataa tctcaatgcg ctgggtagta
    11341 cgctgcaaac cttgtctgac tggtggaagc agttctggga tgcggccatg aatattggtc
    11401 gtgaagactc gctggatgcg cagattgatg cgttacagga aaaaattcag cgcgcgaaaa
    11461 aatatccgtg gacaaacgcc tccacacagg tggagtacga tcagcagcgt cttaacgatc
    11521 ttcaggagaa aaaacgccgg aaggatttgc aggatgcaaa agcgcaggca gaacggaatt
    11581 accaggagca acagaaacgc cggaatgctg aaaatgccgc gctgaaccgg atgaatgaaa
    11641 cggaagctgc acgacatcag cgggaaattg cgcgtattaa tgccatgcag tacgccgacc
    11701 aggctgtcag ggatgcggcg atacagcgtg aaaatgaacg ttacgaaaaa gccattaaga
    11761 aaaatacacg ggcaacccgt aatgatgagg ccacccggtt attgctgcag tacagtcagc
    11821 agcaggcaca ggtggaaggg cagattgctg ctgccagaca gtcagcaggc attgccactg
    11881 aaaggatgac tgaagcgcat aaacagcttc tggccctgca gcagcgcatc agcgacctgg
    11941 acgggaaaaa gctgacggca gatgaaaaga gtgtgctggc ccgtaagaat gagctgattc
    12001 aggcgctgac gctgctggat gtgaaacagc aggagctgca gaaacagaca gcgcttaacg
    12061 acctgagaaa aaaaacggtt cagctgacca gccagctggc agacaaagaa cgtgcactgc
    12121 gtgagcagca caatctggat attgccactg caggtatggg ggataagcag cggcagcgct
    12181 accaggcaca gttgcgcatc cggcaggaat accggcaaca gttgcaacag cttgagaatg
    12241 acagtcgcca gaaaggcact tacgggacgg aggactaccg gagggctgag gaggtgctga
    12301 aggggagcct gaagcgacaa ctgaatgaaa acaaacgcta ctggcaggaa ctggaagtgg
    12361 cgcagggcga ctggaaaaac ggtgccatgc gggcgtttca gaattttacg gcggatgcgg
    12421 ataatgcggc gggaacggca gaacagatgt ttacagtggc attcagcagt gccggtaatg
    12481 cactggcgac attctgtacc accggtaagc tgaattttaa atccttcacc tcttccctgt
    12541 tgtcagatat ggccagaatt atggcacaga tggccatgat gcaggcggta aagggcgtcg
    12601 gttctttatt cggcttcacg actaatgctg atggcggtgt ttaccagtct gctgatttga
    12661 gtcgctacag tggcacggtg gttaaccgtc cgacgttttt tgcttttgca aaaggcgcgg
    12721 gtgtgatggg ggaagcggga cctgaagcca ttctgccact gcgtcgcggt gctgatggta
    12781 agctgggggt tgtggcggat actggtggtt caggcatggt gatgtttgcc ccgcagtaca
    12841 acattgagat caataacgac ggcacgaacg ggcagatagg tccggctgcc ctgaaggtgg
    12901 tttatgacct cgggaaaaaa gcggccgcgg actttatgca acagcaggcc cgtgatggtg
    12961 gccggttaag tggagcatac cggtaatgga gacgtttcac tggaaagtgc gcccggatat
    13021 gaatgtggta tcagagccga aagtggtgac agtgaagctg ggcgatggtt atgaacagcg
    13081 tcgtgcggcg ggactgaata accagttgtc gacttacagc gtgacgatac gtgttcgtaa
    13141 aggtgaacac ccatctttaa aagcctttct ggaacggcac ggtggcgtcc gcgcatttca
    13201 gtggacgcca ccttatgact ggaaactgat cagggtggtt tgtcgtaaat ggtcggcaag
    13261 cgtgggggcg ttatgggtga ctgtaacggc cgattttgaa caggtggtga actgatgcag
    13321 gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcagc ccgggtggag
    13381 ctctgggaaa tcgatctgac agaggtcggt ggtgagcgtt attttttctg taatgagcag
    13441 aacgaaaaag gtgagccggt cacctggcag gggcggcagt atcaggcata ccccattcag
    13501 gggtcggggt ttgaactgaa cggcaggggc tgtgctgccc gtccgacact gacggtctct
    13561 aacctgcacg gcatggtcac cgggatggcg gaagatctgc agagtctggt cggcggaacg
    13621 gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac
    13681 agcgacgccg atccggaaca ggaggtgatc agccgctggc gcatcgagca gtgcagcgaa
    13741 ctgagcgcgg tcagtgcctc ttttgtgctg gccacaccaa cggagacgga tggcgcggtt
    13801 ttcccggggc gtatcatgct ggcgaatacc tgtatgtgga cttaccgttc tgatgagtgt
    13861 ggttacacgg gcagggcagt ggctgacgag ttcgacaaac caacaacgga tatccggaag
    13921 gacaaatgca gcaagtgtat gcgcgggtgt gagttgcgca acaataccgg taatttcggc
    13981 ggtttccttt ccatcaataa actttctcag taaatccatg acacagacag aatcagcgat
    14041 tctggcgcac gcccggcgat gtgcgccagc ggagtcgtgt ggcttcgtgg tgagaacgcc
    14101 ggagggagac aggtatcttc ccagcgagaa tatctccggt gagccggagg aacggttccg
    14161 gatggctccg gaggactggc tgcgggcaca aatgcagggt gagattgtgg cactggtcca
    14221 cagtcatccc ggtggtctgc cctggctgag tgaggctgac cggcggctgc aggtgcagag
    14281 tgatttgccg tggtggctgg tctgccgggg ggcgattcac aagttccgct gtgtgccgca
    14341 tctcaccggg cggcgctttg agcacggggt gacggactgt tacacgctgt tccgggatgc
    14401 ttatcatctg gcggggattg agatgccgga ttttcatcgc ggggatgact ggtggcgtca
    14461 cggtcagaat ctctatctgg ataatctgga ggccacaggg ctgtatcagg tgccgttgtc
    14521 atcagcacaa ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc
    14581 cgccatttac tgcggcgatg gtgagctgct gcaccatatt cctgaacaac tgagtaaacg
    14641 agagaggtat accgacaaat ggcagcgacg cacacactcc ctctggcgtc accgggaatg
    14701 gcacgcatct gcctttacgg ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg
    14761 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggcgtt tcgtcagaaa
    14821 ctgagtgacg gctggtatca ggtgcgcatt gccgggcgtg atgcaggtga aaccgaattg
    14881 tctgcccgtc ttaatgagcc gctggcaaat ggtgcagtga tccacatagt accgcgtctg
    14941 gtgggagcta aaagtggcgg tgtgtttcag gcggtgctgg gggcagctgt tatggcggtt
    15001 gctatatgga tgccgggggt aggaattatg gcgagtaatc tgctgttttc tctcggtgcc
    15061 agtatgacgc ttggcggtgt tgcacagatg ctggcaccga aagccagaac tccccgtaca
    15121 cagacaacgg ataacggcaa acagaacacc tgtttctcct cactggataa catggttgcc
    15181 cagggcaatg ttctgcctgt tctgtacggt gaaatgcgcg tggggtcgcg ggtggtatct
    15241 caggagatca gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat tggtcgctga
    15301 tgcaaaatgt ttcatgtgaa accgcctgcg ggcggttttg tcgtttatgg agcgtgagga
    15361 atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaaatcc
    15421 acgcagttgc tgagtgtgat tgatgccatc agcgaagggc cggttgacgg tccggtggat
    15481 ggattaaaaa gcgtgctgct gaacggtacg ccggtcctgg atagcgaggg gaagacaaac
    15541 ttttccggtg ttacggtggt gttccgcgcc ggcgagcagg agcagacacc gccggagggg
    15601 tttgaatctt ccggctcaga gactgtgctg ggtacggaag tgaaatacga cacgccgatc
    15661 acccggacca tcacgtcggc aaacattgat cgtctgcgcc tgaccttcgg tgtgcaggca
    15721 ctggtggaaa ccacctcaaa gggggaccgg aatccgtcgg aagtccgcct gctggttcag
    15781 atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccact
    15841 tcgcagtatc tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt taatatccgg
    15901 atgcgcagga tgacaccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg
    15961 tcgtacaccg aaatcatcga tgtgaaacag tgctacccga acacggcgct ggtcggcgtg
    16021 caggtggatt cagagcagtt cggtaaccag caggtgagtc gcaattatca tcttcgcggg
    16081 cgcattctgc aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg
    16141 gacggaacgc ttaagccagc atacagcaac aacatggcct ggtgtctgtg ggacatgctc
    16201 actcatccgc gctacggcat ggggaaacgt cttggtgcgg cagatgtgga caaatgggcg
    16261 ctgtatgtca tcggccagta ctgcgaccag tcagtgccgg acggatttgg cggcacggag
    16321 ccgcgcatca cctgtaacgc ttacctgacc acacagcgta aggcgtggga tgttctcagt
    16381 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg
    16441 caggaccgac cgtcggataa agtgtggacc tataaccgca gtaatgtggt gatgccggat
    16501 gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa tgtcgttgag
    16561 gtgaactgga ttgatccgga caacggtcat gagacggcga cagagcttgt ggaggacacg
    16621 caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc
    16681 cggggacagg cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag
    16741 accgtggact tcagcgtcgg cgcagaaggg cttcgccatg taccgggtga tgtcattgaa
    16801 atctgcgatg atgattatgc cggtatcagc accggtggtc gcgtactggc ggtgaacagc
    16861 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc cgtcctccgg taccacgctg
    16921 ataagcctgg ttgacggaaa tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac
    16981 ggcgtgaagg tgaaagtgag ccgggttcct gacggcgttg cagaatacag cgtgtggggg
    17041 ctgaagctgc cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg tgagaacgac
    17101 gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat
    17161 aacggggcac actttgacgg tgaccggcgc ggcacggtga atggtgtcac gccgccagcg
    17221 gtgcagcatc tgaccgccga agtcaccgca gacagcgggg aatatcaggt gctggcgcga
    17281 tgggacacgc cgaaggtggt gaagggtgtg agcttcctgc ttcgcctgac cgtggcagcg
    17341 gacgacggca gtgagcggct ggtcagcaca gccaggacga cggaaaccac ataccgcttc
    17401 aggcagctgg cgctggggaa ttacagtctg acagtccggg cggtaaatgc ccgggggcag
    17461 cagggcgatc cggcgtcggt atcgttccgg attgcggcac cggcagcgcc tgtcactatt
    17521 gaactgatac cggggtattt tcagataacg gtggtcccga aactggctgt atatgacccg
    17581 acggtgcagt ttgagttctg gttttcggaa aagcggattg cggatatcag gcaggttgaa
    17641 accagcgccc gctatcttgg cacggcgctg tactggatag ccgccagtat caatatcagg
    17701 ccgggccatg attattattt ttacgttcgc agtgtgaaca ccgttggcaa atcggcattt
    17761 gtggaggccg tcggtcgggc gagcgatgat gcggaaggtt atctgagttt ttataaaggg
    17821 ttgatcaata aaacgcatct cggcaaggag ttgtggacgc agattgataa cggtcagctt
    17881 gcgccggacc tgactgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcaa
    17941 accgtcaata aaaaactgga aaatcagagt gctgcaatcc agcagataca gaaagttcag
    18001 gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcaaca gatgaaggac
    18061 ggacgccttt atattgcgtg gtatcggtgc cggtattgag aatacgccag caggaatgca
    18121 gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt aatcctgcga atggcaacac
    18181 aaagccgatg tttgttggtc agggcgatca gatattcatg aacgacgtgt tcctgaaacg
    18241 cctgacggct ccgaccatta ccagcggcgg taatcctccg gcattttccc tgacacctgg
    18301 cggacggctg acggcgaaaa atgccgatat cagcggtaac gtgaacgcga actccgggac
    18361 gctcaacaac gtcacgatta ataagaactg tcgggttctg ggaaaattgt ccgcgaacca
    18421 gattgaaggc gatctcgtta aaacagtggg caaacctttc ccacgggact cccgggcacc
    18481 ggagaggtgg ccatcaggga ccattaccgt cagggtttat gacgatcagc cgtttgatcg
    18541 gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa catgagcgga agaataacaa
    18601 tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt gctgaaattt ataaccgaac
    18661 gaccctggat aatacgctga tatatacggg tgttattgat atgcctgccg gtcacggtca
    18721 catgacgctg gagttttctg tatcggcatg gctggtaaat ggctggtatc ccacagcaag
    18781 tatcagcgat ttgctggttg ttgtgatgaa gaaatccaca gcaggtatca gtatcagctg
    18841 aattttataa cccagatacg ggcaccagaa atggtgcctt ttttattgca gaaaagcgag
    18901 aggtaattat gcgtaaactt tatgctgcca ttttgtccgc agccatctgt ctggctgtat
    18961 ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct gagcgcgggg tatcttcatg
    19021 cctcgacgaa cgctcccggc agtgatgatc tgaacgggat taacgtgaaa taccgttatg
    19081 agtttacgga cacgctgggg ctggtgacgt cattcagcta tgccaacgct gaagatgagc
    19141 aaaaaacgca ttacagtgat acccgctggc atgaggattc cgtgcgtaac cgctggttca
    19201 gcgtgatggc ggggctgtct gtgcgcgtga atgaatggtt cagcgcgtat gcgatggcgg
    19261 gcgtggctta cagccgtgtg tcgaccttct ccggggatta tctccgcgta actgacaaca
    19321 aggggaaaac gcacgatgtg ctgaccggga gtgatgacaa ccgccacagc aacacgtctc
    19381 tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc cgtggccatt gaccttgctt
    19441 atgaaggttc cggcagtggc gactggcgca ctgacggttt catcgtgggt gtcggttata
    19501 agttctgatt agccaggtaa cacagtgtta tgacagcccg ctggttcagg cgggcttttt
    19561 gtggggtgaa tatggcagta aagatttcag gtgtactgaa agatggggcg ggtaaacctg
    19621 tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag tccgaccgtt gtggcacacg
    19681 ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt gattgaggct gagccggggt
    19741 attacgaggt tgcgcttcac tgtaacggct ggcagccaac ccgtgtcggg gatattgatg
    19801 tggcaccgac tgatgcaccg gggacactga acgcgtttct gaatgcacca aaggatggtg
    19861 atttacgtcc agaggtgatg aagcgctttg aggaaatggt ggcgcaggcg cagcagagtg
    19921 ccggggc
//
LOCUS       DAJVRP010000082        19414 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVRP010000082 DAJVRP010000000
VERSION     DAJVRP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.082"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="OQN12_002908"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="OQN12_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="OQN12_002909"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="OQN12_002909"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1655155.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="OQN12_002910"
     CDS             2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="OQN12_002910"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1655156.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="OQN12_002911"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="OQN12_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1655157.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="OQN12_002912"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="OQN12_002912"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1655158.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="OQN12_002913"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="OQN12_002913"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1655159.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="OQN12_002914"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="OQN12_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1655160.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="OQN12_002915"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="OQN12_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1655161.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="OQN12_002916"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="OQN12_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1655162.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="OQN12_002917"
     CDS             11402..11617
                     /locus_tag="OQN12_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1655163.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="OQN12_002918"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="OQN12_002918"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1655164.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="OQN12_002919"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="OQN12_002919"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1655165.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="OQN12_002920"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="OQN12_002920"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1655166.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="OQN12_002921"
     CDS             complement(16353..16892)
                     /locus_tag="OQN12_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS1655167.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="OQN12_002922"
     CDS             complement(16908..17426)
                     /locus_tag="OQN12_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1655168.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17737..17928)
                     /locus_tag="OQN12_002923"
     CDS             complement(17737..17928)
                     /locus_tag="OQN12_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCS1655169.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17946..18098
                     /locus_tag="OQN12_002924"
     CDS             17946..18098
                     /locus_tag="OQN12_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655170.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18280..>19414
                     /gene="pdeF"
                     /locus_tag="OQN12_002925"
     CDS             18280..>19414
                     /gene="pdeF"
                     /locus_tag="OQN12_002925"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1655171.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4568 a         4729 c         5278 g         4839 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgtc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cactgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 attggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtcgatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt
    17521 attaaaattc acgcagggaa taatttataa aaatatatca ttacaacata tatattaatt
    17581 ttatatttat ttcgcagcca aacaggataa acatcagtca ggacaaagac gtaaaattta
    17641 agatgaaaga atatctgccg ctttgataat cccaaagagc aaaagcatcc gacagcgaag
    17701 tgccgttatg ttctgtgaaa gtggagaagg taaccgctaa cgttgtaccg gtgattcgac
    17761 ggagagtgtg gattgctgag cttctttttt gctctgatgg tgctgccagg caccgacgga
    17821 cgagtagata aaacggccaa agaagaagat aaagctgatg agcagtacga tacgggtcat
    17881 gcgactgtta aatcggtgtc gttttcgcat actagttgcc tgactcacaa aaggttcctt
    17941 gaagtatgtc ccacgctgtg gacggtactt acattaaggc acaacaggac aaaatggtca
    18001 attcttcgtt atgtaaaaaa gcgtcagtgg atacatattt taatgttatg gaagttaatt
    18061 taattattta caatgatgat gtaataatga tgaattaaca tgataaacgt aataattcat
    18121 taatcagggt tattttgttt gatatatatc aattggaact ttcatatgac acttagaatc
    18181 aatgctctct atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat
    18241 acacctcctt caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat
    18301 ataaaaatac gtgataaatg gtgggggctt ccgctgttcc tgccttcttt aatcttgccc
    18361 attttcgccc acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat
    18421 ctgcctttgg cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg
    18481 atcaccttag ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctctca
    18541 ttaacggcta attttattat cattatcatt ctctgttggg gcggttacag ggtctttact
    18601 ccccggcgta acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg
    18661 cagatagtgt ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga
    18721 ttactggcga gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc
    18781 aattatcagg ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt
    18841 cgggtagtgc gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt
    18901 gatgccaaag tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg
    18961 ttgctgttat gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg
    19021 tcattattgc tgcccctgat gatgtgggga gcaatgcgct atggttataa gctgatttcg
    19081 ctgctctggg cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt
    19141 tatcctggct ataccacgca gctgaccata acctcctcca gttatctggt attctctttt
    19201 attgtcaatt atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag
    19261 cggcttgcgt atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg
    19321 ttgcgtgatg ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg
    19381 gttaagaact atggcatcat gctgcggatt caat
//
LOCUS       DAJVRP010000083        19261 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVRP010000083 DAJVRP010000000
VERSION     DAJVRP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.083"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            31..330
                     /locus_tag="OQN12_002926"
     CDS             31..330
                     /locus_tag="OQN12_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1655172.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            327..2075
                     /locus_tag="OQN12_002927"
     CDS             327..2075
                     /locus_tag="OQN12_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1655173.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2364..2642
                     /locus_tag="OQN12_002928"
     CDS             2364..2642
                     /locus_tag="OQN12_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655174.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2639..2944
                     /locus_tag="OQN12_002929"
     CDS             2639..2944
                     /locus_tag="OQN12_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655175.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2954..3115
                     /locus_tag="OQN12_002930"
     CDS             2954..3115
                     /locus_tag="OQN12_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1655176.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3257..3481
                     /locus_tag="OQN12_002931"
     CDS             3257..3481
                     /locus_tag="OQN12_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655177.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3782..4939
                     /locus_tag="OQN12_002932"
                     /pseudo
     CDS             3782..4939
                     /locus_tag="OQN12_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4979..5551
                     /locus_tag="OQN12_002933"
     CDS             4979..5551
                     /locus_tag="OQN12_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1655178.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5553..6764
                     /locus_tag="OQN12_002934"
     CDS             5553..6764
                     /locus_tag="OQN12_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1655179.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6761..7099
                     /locus_tag="OQN12_002935"
     CDS             6761..7099
                     /locus_tag="OQN12_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1655180.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7096..7392
                     /locus_tag="OQN12_002936"
     CDS             7096..7392
                     /locus_tag="OQN12_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1655181.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7392..7832
                     /locus_tag="OQN12_002937"
     CDS             7392..7832
                     /locus_tag="OQN12_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1655182.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7816..7998
                     /locus_tag="OQN12_002938"
     CDS             7816..7998
                     /locus_tag="OQN12_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655183.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8054..8344
                     /locus_tag="OQN12_002939"
     CDS             8054..8344
                     /locus_tag="OQN12_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1655184.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8364..10025
                     /locus_tag="OQN12_002940"
     CDS             8364..10025
                     /locus_tag="OQN12_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1655185.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10039..10320
                     /locus_tag="OQN12_002941"
     CDS             10039..10320
                     /locus_tag="OQN12_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655186.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11117..12577)
                     /gene="phoQ"
                     /locus_tag="OQN12_002942"
     CDS             complement(11117..12577)
                     /gene="phoQ"
                     /locus_tag="OQN12_002942"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1655187.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12577..13248)
                     /gene="phoP"
                     /locus_tag="OQN12_002943"
     CDS             complement(12577..13248)
                     /gene="phoP"
                     /locus_tag="OQN12_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1655188.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13417..14787)
                     /gene="purB"
                     /locus_tag="OQN12_002944"
     CDS             complement(13417..14787)
                     /gene="purB"
                     /locus_tag="OQN12_002944"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1655189.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14791..15432)
                     /gene="hflD"
                     /locus_tag="OQN12_002945"
     CDS             complement(14791..15432)
                     /gene="hflD"
                     /locus_tag="OQN12_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1655190.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15468..16574)
                     /gene="mnmA"
                     /locus_tag="OQN12_002946"
     CDS             complement(15468..16574)
                     /gene="mnmA"
                     /locus_tag="OQN12_002946"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1655191.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16628..17089)
                     /gene="nudJ"
                     /locus_tag="OQN12_002947"
     CDS             complement(16628..17089)
                     /gene="nudJ"
                     /locus_tag="OQN12_002947"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1655192.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17099..17752)
                     /gene="rluE"
                     /locus_tag="OQN12_002948"
     CDS             complement(17099..17752)
                     /gene="rluE"
                     /locus_tag="OQN12_002948"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1655193.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17924..19174
                     /gene="icd"
                     /locus_tag="OQN12_002949"
     CDS             17924..19174
                     /gene="icd"
                     /locus_tag="OQN12_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1655194.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5015 a         4728 c         5159 g         4359 t
ORIGIN      
        1 taaagaacaa cacaccggag aataaaaata atgaaactta aatattctgg cttaactgcc
       61 agtggcaaca ctcaccctaa atttacgcgc ggtgatattt accgcgacca gtacggcggc
      121 atggtaacga ttaaaggcgt ggcagaacgg cgcatcacct accgccgtga aggttacgaa
      181 tatgattgcg tgatgcctgt ttatcaattc cggcgtgatt tcacgctggt acaggcagcg
      241 ccccgcagta aaccaaccag cagggagaaa gcacgcgcca atattcagga aataaaaaag
      301 atgcttaacg tattcagggg taaaaaatga aactggcacc gaacgtaaaa aaacagccac
      361 gcggaataaa acacaaagac acagaggtaa ttatctttgc gggtagtgat gcctggtcgc
      421 acgcgaaaca atggcaggag caggatggcc ccgcatccgg cgataatgtg ccgcctgtgt
      481 ggcttgggcc aaatcagctt gccgaacttg atgcactgaa aattgttccg gatggaaaaa
      541 aacgcgtaag gctgtaccag gccggagaac tggatttggt ggagaccaaa aagattggtc
      601 agaagctggc ggcggcagat attcaggacg caaattttta ccccgaagga atgcacgtcc
      661 agaagtgtga aaactggcgg cgctatctga atgctgagcg tgaaaatatt gccgcagggc
      721 ttaccatgcc ggagcagaaa aatacgcaac tggcacaaat ggcagacagt gagcgcgcgc
      781 agctgcttgc cgagcgcttt gatggcgttt gtgtgcatca ggaaagtgaa atcgttcacg
      841 tatggcgcgg cggggtatgg tgtccggtca gcacaatgga acttagccgc gaaatggtgg
      901 cgatctattc agagcacagg gccactttca gcaagcgcgt aatcaataac gccgtggaag
      961 cgttaaaagt tattgccgaa cccatgggcg agccgtccgg cgatttgctg ccgttcgcca
     1021 atggtgcgct tgacctgaaa acgggggaat tttccccgca cacgccggag aactggatca
     1081 ccacgcacaa cggcattgag tacacgccac cagcaccagg ggagaacatc cgcgataacg
     1141 cgctaaactt tcataaatgg cttgagcacg cagccggaaa agaccagcgc aagatgatgc
     1201 gtatatgtgc cgcgctgtac atgattatgg cgaaccggta cgactggcag atgtttattg
     1261 aggccaccgg agacggcggg agcggtaaaa gtacattcac acacatagcc agccttctgg
     1321 cagggaaaca gaacacggta agcgctgaaa tgacatcgct tgatgatgct ggtggacgtg
     1381 cgcaggttgt cgggagtcgt cttatcgtcc tggctgacca gccgaaatat acaggcgaag
     1441 gaacgggcat caagaaaatc acgggcggtg accccgcgga aattaacccg aaatacgaaa
     1501 agcgtttcac ggctgtaatc agggcggtgg tgctggcgac caacaataac ccgatgatat
     1561 tcaccgaacg ggccggaggt gtggcacgtc gtcgcgtgat tttccgtttc gacaatatcg
     1621 ttagtgaggc cgaaaaagac agggagctac cggaaaagat tgcggctgaa atccctgtca
     1681 ttatccgccg attgctggcg aactttaccg actctgagaa agcacgggct ttactactgg
     1741 aacagcgtga cggtgatgaa gctctggcaa taaagcaaca gacggatccg gttattgagt
     1801 tttgccagtt cctgaatttt ctggaggaag cacgcggcct gatgatgggc ggcggtggcg
     1861 attcagtgaa gtacacgacc agaaacagcc tttaccgcgt ctatctggcg tttatggcgt
     1921 acgcaggcag gagcaaaccg ctaaacgtaa atgactttgg caaggctatg aagccagccg
     1981 cgaaagttta cggacatgaa tatattacgc ggaaagttaa gggagtaacg cagactaacg
     2041 caataacaac agacgagtgc gacgcgtttt tataattttt tgtaattgct gtctaccctg
     2101 tctacctgag taaagaaaaa tacatttaat tcagtgcatt aagttaggta gatagccttt
     2161 ttttactgtc tacccactat ctaccctctc tacctgattt tacctgaatc agacagggag
     2221 gtagacacgg ggtagatagt ggataaaagc actctacccc gctgaaagca gcgccattac
     2281 tggcatgagg gccactaagg tagataaggt agacaagggg aggcacaact caaaactttt
     2341 taaacgaggg ggtaaaaata aatatgcaca cttcagggaa atttaataaa tcattcaaaa
     2401 aacgcagaga cagaacagaa ccgaaatatc gcgcgttaga catgacagag cacgctttaa
     2461 aggtggcaat cagaacgata gaccgccacg cgggggaagg atacgcgaaa gcacatcccg
     2521 aactgataag cgcattcatg accacgacgg cggcaaattt tgccacgctg acagagcggg
     2581 agattgccga agcggaacag gtaacaacaa tcaacgttaa aaccggagag gtggaatcat
     2641 gagcgaacca aaatttggtg aaaaatttta caagcacaac ggacggatca cgattctgca
     2701 aataagcgca gctacaccag gctggtgggt tgaaacagac gaaggatcct cacccgtggc
     2761 atcatgggcg ctttgtgtgc ttagttaccc tgaccgcgat gtgtatcagg atattctgcc
     2821 tgtgatatcc actgacaaag gcatgaaacc cgtagacatc aaaaacatgg ggtttcagtg
     2881 cgtaatgctc actgaaaaaa tgatggaaga gatgaaaaag aacaatgccg gatcgcttca
     2941 ctgaggtggc aatatgacag cacaaattgc agcttacggg cggctggtgg gcgacccgct
     3001 ggtaaaacag agcagcaagg gcacaccgga gccgccaacg gagaactacg cagacctgag
     3061 aaatgcagta aatcgcactt acgcagacat ggggctgagc aaattagcag agtaggaata
     3121 tcagaaccac accacgcagc cggagcaatc cggctttttt tgcgtcattt gaaacggtat
     3181 ggagcactac tggcttttcg tcacggtccg gcatagttac tatctgaaac aaacagacac
     3241 aacagaggaa aacgcaatgc cgatgaaatt tgatgagata cttaaacagc gtgataaata
     3301 ccatgctgac aacatggaga cgatgaatat cactgattac cgcgcatttc tggaaacggg
     3361 ggcgctgatt gaaaaggatc atcatggttt tgttcgttgc gcgctatccg gtgaaatgct
     3421 ggcagtaaat cctgaacaga cagatgcatt gatagaattt ctgaaaggga tcagagactg
     3481 agcacccata cagcacatac agccggagca atccggcttt ttgttatttt ttgtaaatta
     3541 tttgttcgtg gttgttccac gttgttcact gagcggatcg gcatatttta cccgaactga
     3601 atcatgatta ttctcgcccg tggtgccagg acgctggggc cactttcccg cctgttaatg
     3661 tgctcgccaa tattcattac caggcgggaa aacgatcggt gcgattactg atttccttat
     3721 gaaaaacggt tgagtgtttg ccgcgtcctg gagttcctta cttaacccca ggactttttt
     3781 tatgcagaga ataatcgaat tacgccagca gaaaaccgcc attaaaaatc agatgcgcga
     3841 catgctggag aacgctgaaa aagaaaaccg cagtcttaac gatgctgagg gcacaaaatt
     3901 tgacgaatta cgcgctaaag ctgaatccct cgataaagac atttcccgcc ttgaagccat
     3961 tgcagacgaa gagcgcagca agccaggtaa aagcagccag accactgacc ccgcagaact
     4021 acgcaactac attctgaccg gtgaaacccg cgcattaagt acaggcgttc ccgctgatgg
     4081 tggttatacc gttatccctg aactgaacac cgaaatcatg cgaatgctgg cggatgaatc
     4141 caccatgcgt cgcatctgta ccgtgaagaa aatcagcagc aacgagttta agcagcttgt
     4201 ttccgctggc ggtgcgaccg ttaaccacgg tgaagagggt aaggcacgcg aacagaccag
     4261 caccccgcag attaacgagg tgagcattaa gctgtatccg gtctatgcgt acccgcgcac
     4321 cacacaggaa atcgtggatt tttccgatgt ggacatcctt tcatggctga cgggtgagat
     4381 tggcgacacc ttcacggaaa ccgaagaaag cgatctggtt gtgggcgacg gtgacaaaaa
     4441 agcaaaaggt tttttatccg taccccgtgc agagaagaac gacaaagagc gtgattttgg
     4501 tacgttgcag gtaattaaac cttccgaatc tctggcgtgg acatctgcgg acccgctgat
     4561 cgacctgaaa tttgcattac gtaaaaaata ccgcaaaaac gcggtctgag tggttaactc
     4621 cacgacggcg gcaaaacttc agaaggtgaa gaacgcgaac ggtgattata tctggcgcga
     4681 ccgtttacag acgggtgatc ctgatacgtt gctgggcctt ccggtcgaat atctggagtt
     4741 tatgcctgat aacgttattg ccctgggtga cttcaaacgc ggttactaca ttgttgatca
     4801 cgaaacaggt gttcgcacca gaccggacaa cctgacagaa ccaggattca tcaaaatttt
     4861 cacgcaaaaa tatttaggcg gtggcgtggt ggattcgaac gcgatcaaga ttctggcact
     4921 gccggaagac gacgattaac agcatacaga aggggctaaa aagccccttt agtgttttat
     4981 gggtgaaaaa attatgaaga gtatggaaat ccggtcatcg gaaatcacca ccagcggagc
     5041 cggtacgctg acgggctacg ttgttcgctg ggataagctt tcagaactgt tatggggtga
     5101 gttttacgaa aaattccagc ggggggcgtt tactgaatgg cttgcggcgg gtaatgacgt
     5161 tcgcggcctg tatgagcatg accacagcat gttactgggg cgcacccgtt ccggcacgct
     5221 gaaactggaa gaggacgaaa cagggttacg ctttgaactg accccaccgg ataccagtac
     5281 agggcgcgac gttatcgaac tggttaagcg cggggacatc tcggggatga gctttggctt
     5341 tcgctcccgt aaggatgtat gggataccac aacagatcca tgcgtgcgca ccgtgctggt
     5401 ggcggaactg tacgaaatta ccgttacatc ggtaccggct taccccgatt ccggcgtgga
     5461 gctggcccgc cgttccctgt atgagcagca ccccgaaaaa atgccgcgtg cggataatcg
     5521 ccgctggtgg gcggatttag cgggggtgtg atatgtggcc tttcagacga aaaaaagagc
     5581 agcgcagcat gacgcttgat gaatttatgg cgctggccgg cacatcgaac acgggggcgg
     5641 gtgagtacgt atcatctggg acagcggaat cactccccgc cgtcatgaac gccgtcacgg
     5701 tcatctctga cgcggtggct accatgccgt gttacctgta cctggtacgc aatgagaagg
     5761 ggaaggaggc ccgcgaatgg cttgattctc atccggtcga tcacatcctc aacgagcgcc
     5821 cgaacgcgtg gcagacaccc taccagttta agcgaatgat gatccgctac tgcctgttaa
     5881 gtggtaatgc ttatgcggtt attcagtggg ggcgtgatgg ttttccggcg gctttacatc
     5941 cttacccgcc gcagtcggtg aacgtggagc agacaggtga tcataactgg cgctactgca
     6001 tcactgacgc ctacaccgga aacacccgca actatttacc gtgggaggtt ctccaccttc
     6061 gttactccac ggatgacggt tttatggggc gctcacctgt aaccatctgc cgcgaatcgc
     6121 tggggcttgg gctggcccaa caacgccacg gcgcgagcgt gatgcgtgat ggcatgatgg
     6181 cggcaggggt tatcacgtca ggcgaatggc tggacggcgt gaagggcaaa caggcattag
     6241 ccgcactgga acgctacaaa ggggccagaa acgccggaaa aacgcccatc cttgaagggg
     6301 ggatgagcta tcagcagctg ggcatgagta atcaggatgc cgaatggctg gcctcccgtc
     6361 gcttcaccat tgaagacatc gcccgaatgt tcaacgtctc gccgattttt ttgcaggaat
     6421 acagcaacag cacctacagc aacttcagcg aggcaagccg cgcatttctc accatgacga
     6481 tgcgcccgtg gctggcgaac tttgagcagc agataaaaaa cgccttgctg gtggcctcgc
     6541 ctgtacctgg tatccggtat caggtggagt ttgacagcgc ggacctgtta cgggccacac
     6601 ctggcgaacg ctttgccacc tatgagcgcg gcataaaatc cgacgttatg tgtccgaacg
     6661 aagcccgcga acgtgaaggg ctgtcaccgc gtgatggtgg tgatgagttc agccaggcat
     6721 ggaaacagga agtaaaaatc agcgagggag aaaaaccgga atgaacatag ggcgactgcg
     6781 tgacaggata acgattcaga ccctgaaaca gaccagggat ataaccggcg aaatactcga
     6841 aacgtgggag gacggtcaca cactctgggc aagcgtgaac atggtcagca gcaaggaggc
     6901 catttcatcg ggcgcagagc tggcgattgg taccgtaagg atctggatac ggtaccggaa
     6961 ggacatcaac gccaccagcc ggataaaggt cagtacgggg ccgctggcgg ggcgtgtact
     7021 gaatatcatc gggcagccgc tgccggatgc cgccaggaca cgccttgaaa ttctttgtcg
     7081 tgagggcgcg gaaaaatgac agaagaactt atcaccctgg aagaagtgaa actccattgc
     7141 cgcatcgatg gcgacgatga agaccagtta atcagcggat acattgccgc atcgcttgag
     7201 gcgtgccaga tacacatagg caggcgcttt gatgacgggc tggcgttcac gccagccata
     7261 aagattggct gcatgatgtt tatcgctcac ctgtatgaaa atcgccagat tgtcgcggat
     7321 aacgcaaaaa cacgcgtacc catgacgata ggcgcactct ggacggctta ccgtgatgtg
     7381 ggggggtact gatgccgtgg caaccattaa gacgatgcac agaaccaggc tgtaacaggc
     7441 gcgttaaatc cggcaagtgt gaggagcaca ggcgggcggc atggcgtgca gaggatgcca
     7501 gacgaggaca ccgccgcgcg cgtggatatt ccagacagtg ggacaaatac cgcgccatgt
     7561 atctgagtaa aaacccgtta tgcgtgcgat gccttgagaa ggggatatat acgcccgccg
     7621 tggtggtgga tcacattatc ccgattaatg gcggtgatga tgttctcttc tggcccgaat
     7681 ggaaccatca accattgtgc caggcgtgtc ataaccagaa aacgaaatgg cttgatccgg
     7741 caacaaaaag caagcgtgcc gcaggtggat ttcgtgaaga ggaagaacgg gctgctaacc
     7801 gcaataactg gatgtatgac gctgatgaat gagcgggaac aaaaccgcct tatccgtgga
     7861 cttataaggc agcgtgacgc atggaagaca caggaaacag ggcataaaga taaagcgtca
     7921 ggacgcgcag aacgcatcac agcgaagcga ttaaccgacc gtgaccgcga ggttatggaa
     7981 tgtttccgca atcgctgatg aggctgtatg acggggtggg gggcgttttc aggacaaacc
     8041 cgaccccgcc tacctggatg atatcgccgt gaagcagtgg cgggaaaaat cgcggcagct
     8101 tgcggaacag ggagacctga cccccgccga ctggagcaat ctggaactgt attgcgtcaa
     8161 ctactccatt taccggaaag ccgttgcaga ccttgcggcg cgcgggttca gcattgttaa
     8221 cagtcagggc ggcgagagca gaaaccccgc attaagcgca aaatccgacg ctgaaagagt
     8281 gatgataaaa atggcctcct tgctcggttt tgacccgata agccgccgta aaaatccacc
     8341 gtaaacagaa gaagaggacg agcttgaccg cctggaataa gtacgcagaa gacgtaaaaa
     8401 cgggcaaaat tccggcctgt aaacggttaa aacaggctgt taaacggtac ttttcggacc
     8461 ttaaaagccc cctttacacg ttcgatcgtg aggttgtgga gcggtttatt gccttttcca
     8521 gggtgtgccc gcacgtaaaa ggaccgatgc gtggcagacc cattgagctg gagccgtggc
     8581 agcagttcgc ctttgcgtgc atcctcggct ttaaggttaa ggccaccgga cggcgcaaat
     8641 acacgagcgc ctttatcgaa gtgccgcgca aaaatgcgaa atccacgacc gccgcgattc
     8701 tggctaactg gtttctgatt atggagaacg gtcagcagga tatctacacc gcagcggtga
     8761 gccgtgacca ggcgcggatc gtgtttgata atgcgcgtca gatgtgcctt ttatcccgac
     8821 cgttacgcag gcgggtgaat attcaggcgc ataaggtgat acacccgaaa agcaacagcc
     8881 tgttaaagcc gctggcagca aaagcggcaa ccatcgaggg gacaaacccc agtctttcca
     8941 tcgtggacga ataccaccta caccccgata acggcgttta ttccgcgctt gagctgggga
     9001 tgggcgcacg tcccgaaggg ctgttatttg ccattaccac atcgggcagt aacgtcgttt
     9061 cagcctgtaa acagcactat gattattgct gccagatcct ggacggcgaa gaggtcaacg
     9121 attcaatttt tgtactgatt tacgaactgg acgacgaaaa cgaggttgat gatccggcga
     9181 tgtggataaa ggcgaaccct aatattgatg tttccgtgga tcgtgaaaaa ctggcctcaa
     9241 ccatccagaa agcgcggggt attccgtcgc agtgggtgga gatgatgaca aagcgattta
     9301 atatctggtg tcaggggact accccgtgga tgggtaatgg tgcatgggct gagtgtgccg
     9361 gaacgttcac ggaggaagat ttacacggtc aggagtgtta cgcggggctg gacttatcat
     9421 caaccagcga tatttccagc gtatgctatg cctttccggt cggtaaaacc attatgctga
     9481 tttcccgtca ttatctgccg gagttccagc tacagaaccc cgccaataaa aaccgcgctg
     9541 tctatcgcca gtgggctaaa gcgggctgga tacgcacaac gccaggtgac tgcattgatt
     9601 acgaccggat cagagatgac atcatgcagg atgcggagaa atttaatatc aggctggtgg
     9661 gctttgatac gtggaacgcc acgcatctca ggacgcaatt acagggggct ggttttgagg
     9721 tggagccgtt cccgcaaacc tacctcagat tcagtccggc ggcgaaatcg ttcgaagttt
     9781 ttgttaaccg cagggtgatt gtgcatcgtg gcgatccggt gttgtcctgg tcgatgagta
     9841 acgtcgtgat gcagagtgac gcgaacgcca atatcaagcc gaacaagaaa aaatcaccga
     9901 acaagataga cccgagcgta gcggcgctga tggcgtttgg tacattccag gcagagcatg
     9961 aggattttgc ttttgatata agcgacagcc accgccagaa actggaagaa tttagcgggg
    10021 tgtaatgagg tcagcagcat gacagaagcc gaaatactgc gattaatccg ccgcgtctct
    10081 ggaatcagcc agcaggctga cgaacagacc acgcagccgg acagcgtgac agccgaaaat
    10141 tatgcgcgtg ttgttgctga ggtgatgcgc cgtgatggta tccagcttaa tgatgcggat
    10201 atgcgcgaca tacggatccg cgttctggaa atgctggcct acaatcgccg cgttgaactg
    10261 tatcgggaga aggagaaaat cacgtaccac tggaagaagc cggagcggtt acggcggtaa
    10321 ctcactgaga ttaaagcaaa gcgcaaaatt gcgctggcta catccctcac atctgaggac
    10381 tgttaaccag caggccggaa tatattcaga aattacgaaa ttttcgcagt ttaattactc
    10441 gtcagcttaa tcgctaaccc gctctgatat tttcggaaac ctcaatttga ggaagtcggc
    10501 gaggtaactg tttgatattc ccgatagcgc aaaaatgcgc ggtcgggcag aatgaccacc
    10561 acctcaatcg tgacggagag tgacaaccag tgacacaccg tgacaaaaag acggtttaac
    10621 cgttcattaa aaatcgacgt gattttgtgc tttccggatg acgtggccaa catcattttt
    10681 tagcgaaaaa ttctgattaa ggtcactggc tgaacggtca taatgaccac gcagaccatc
    10741 gaggcaacca tagcgatcat cgttacggtt ggagcttttc cgccattggt ggaaaaggtg
    10801 cacatctgga tcatcattac ggtgatcaag ggtatgtaaa acccaccagc ctgattaaag
    10861 gttaaccgga aaaaaaatcc ggtagccccg ttatggggat ccccatatcg acattaacgc
    10921 ccctcatgaa ttggtgcgct tccccctgga aagattaccc gcctgtatat ttcttgtgtc
    10981 tatttgttcc acgttgtttc atacagtgca ccgaacggtg tagttaatgg tgtagtcaat
    11041 ttgcgatttt tggcactttt tcaatgaaaa ttgtttgatc gaatcaatga cttgatgtag
    11101 tggtaaaagg acatatttat tcatctttcg gcgcagaatg ctggcgacca aaaatcacct
    11161 ccatccgcgc accgcccagc atgctctctc cggcgacgat tttaccctca tattgctcgg
    11221 tgatttcgcg ggctaccgcc agccctacac cttgcccagg gcgtaaagta tcaacccgtt
    11281 gaccacggtc gaaaatgacc tctcgcttgc ttaatggaat accggggcca tcatcctcga
    11341 ccacaatata gagatgctcg tcggtttgcc ttgcagaaat ttcgacaaac tcgaggcaat
    11401 atttacaggc attatccagc acgttgccca tcacctcgac aaaatcgttc tgctcaccga
    11461 caaagctgat ctctggcgaa atatcgagag agatattgac ccctttgcgt tgatacactt
    11521 tgttcagcgc tgaggtgaga ttgtccagca gtggggcgac cggatgcagc tcgcggctga
    11581 gcaatgtccc gccgcgcata ctggcacgat gcaggtagta gccaatttgc tgtgaaatgc
    11641 ggctgatttg ctccagcatt accggctcag catcactgac gctcatcttt tcactacgca
    11701 gagaacgcag cgtactttgc agcaccgcca gtggcgtttt cagactatgg gtcaggtcgg
    11761 tgagcgtcgt acggtatttg tcgtaacgtt cgcgttcact ttttaacaat cggttcaggt
    11821 ttcgtaccag actggtcagt tctcgcgttg tggctggatt gagcaattcg cggttatgtt
    11881 cttccagttc gcggacttct tttgccaggg cttcgatggg gcgtaaactc caccaggcgg
    11941 cgacccacag cagcgggatc actaacagca gattggctga gagcacatag ataaaccagc
    12001 tccagaccat ataggaactt tttagctcca ccggaatggt atccaccacc acaatggtta
    12061 attttggcat ccgcgatgtt gccgggtaga cgtttaccgc caccgagtgg gtcatctccg
    12121 cgtcgtcatc atcttcccgc acttcctgca actgttgctg tatcgaatga tctccactca
    12181 gcaagaggct ggtatcgtta acatccgctt caatttcatg aaaaccattc gatttcagcc
    12241 agtcaggctg gatcattttc atcagccagg gcacgtcacg ttgcgcccat aaaagctgcc
    12301 cgttctcatc ataaattagc gtcatggtgg ggctttgctt gtcgatattt tcgggtaact
    12361 cgacatgcaa cttattgttt tcccactgcg caagggtata gaacagattg ctctcgccac
    12421 gtaacagccg aaacgtagtt ttatcgaaac tgacgctata accgatcagc gcgaccattc
    12481 cgtaggcaag cgaaagcacc agtactaccg ctgccgttgc caacagaaaa cgtacccgca
    12541 gcgagagcgg gaaaaaaaga cgcagtaatt ttttcatcag cgcaattcga acaggtagcc
    12601 ctggccgcga acggtggtaa tgacttcttg cggatattgt gcctgaattt ttttgcgcag
    12661 acgtcccatc agtacatcaa tggtatggct ttcccgcagc tccgcatccg gatagagttg
    12721 tagcattaac gaatctttgc tgaccacttt gccattattg cgtatcaacg tttccataat
    12781 ggtgtattcg aacgcggtca gtttgatcac ttcgtcatta atagataatt cacggcgaga
    12841 gagatcaacc tgaaacgggg ggagcgaaat gacctgtgaa gccagaccgc tattacgccg
    12901 cattaatgcc tgcattcgcg ccatcacctc ttcaatatga aacggtttag tcacgtaatc
    12961 atcagcaccg gcacttaata cttcgacttt gtcctgccag ctttcacggg cggttaatac
    13021 cagaatcggc agtgaaacat cgttgctacg ccagcggcga atcagtgaca gaccgtcctc
    13081 gtcaggcaat ccgagatcga caatcgcaat atccggtaaa tgttcattga gataataatc
    13141 ggcttctttg gcatcttctg cgtcatcgac ctgatgacca gcatcctgaa tctgaacttt
    13201 aaggtggtga cgtaacaacg cattgtcttc aacaaccagt acgcgcattt ttatttctcc
    13261 ctgtcttaat tattaaaata gtgtaacgcg attatgtgtt tatgggggta aacattaaat
    13321 aaaccagcgg ggaggggagg taaagtgaaa aaagaaaaag tggataatct taataagcag
    13381 gccggacagc atcgccatcc ggcactgatt tgaggcttat ttcagctcat caaccatcgt
    13441 gatagctcga ccaatatagt tagccggcgt catcgctttc aggcgggctt tctcttcttc
    13501 tggcaacgcc agaccatcga taaactgctt catgccttcg gcgtcaacgc gcttaccgcg
    13561 agtaagctct ttcagcttct cgtacggttt ttcgatgcca tagcgacgca taactgtctg
    13621 gattggttct gccagcactt cccagttgtg atccagctca tccagcagat ggtcacggtt
    13681 cacttccagt ttgctcacgc ctttcagggt ggattgatac gcaatcagcg cataaccgat
    13741 acccacgccg aggttacgca gcacggtgga gtcggtcagg tcacgctgcc agcgggaaac
    13801 cggcagtttg cttgccagat gctgcaatac cgcgttggaa aggcccagat tcccttcgga
    13861 gttttcgaag tcgatcgggt taactttatg cggcatggtg gaagaaccaa tctcaccagc
    13921 aatggttttc tgtttgaagt ggttaagggc gatataaccc cagacgtcac ggtcaaagtc
    13981 gatcagaatg gtgttgaagc gcgcaacgca atcaaacagt tcggcaatgt agtcgtgcgg
    14041 ttcaatctgg gtagtgtacg ggttccattg aatacccagc gaggtgacga actcttcgct
    14101 gaactgatgc cagtcaactt ccgggtaagc ggcgatgtgg gcgttatagt taccgaccgc
    14161 gccgttgatt ttgccgagga tctccacctg gttaagctgg cggtgctggc gctccatacg
    14221 gtaggcgacg tttgccatct ctttaccgat ggttgacggc gtggctggct gaccgtgggt
    14281 acgagacagc agcgggatat cgcggtactg aacggcgaga tctttaatgc catcaatcag
    14341 ttgacgccag tacggcagga tcacttcatc acgcgcggtt ttcagcatta atgcgtggga
    14401 gaggttattg atatcttccg aagtacaggc aaagtggatg aattcagaaa ccgcgtgcag
    14461 ttccgggatc tccgccactt tttctttcag gaaatactca accgctttaa cgtcgtggtt
    14521 agtggtacgc tcgatggttt tgatgcgtgc ggcatcttct tcgctgaaat tggcgacaat
    14581 tgcatcaagg taaccgattg cgtcggcagc aaaagcagga acttccttga tcgctgcgtg
    14641 cgcggccagt ttttgcagcc aacgtacttc aacttgtaca cggaatttca gcaaaccata
    14701 ttcgctgaaa atcccgcgca gcgcgctgac tttatcgccg tagcgtccat cgacagggga
    14761 aacggcggtc agtgaggata attccataga tcacaactcc ggggttaaat gagcaagaat
    14821 ttgttttgcc tgagtggtca ggcgattacg agaaaacatc agttgtagac gtccgccgcc
    14881 gacctggtgc cagagcacgg cggcgcgaat gcctgccagc agggttgcgc gaactttcgc
    14941 ctgcacttgt gggctttgca gtacagcagg ggaaccggtg acctgaatgc gcgggccaag
    15001 cgggctgatc acatcaacat agatagcagc catcgcgctc atcagcgttt cggactgtaa
    15061 atcgaagtgt tcgagctggc gttgcaggcc gttgatccgg ttgcccagag tgtcgagcgc
    15121 gcctttcgct gaggagagtt tgcgctcaag caccatcaag ctgagtgtgt agcgggttaa
    15181 ttcggcgttt aagccctggc aactgctggc attgagcacg ccgagcaggg tttccagccc
    15241 gacgcgcagg ttggcttcgc taccgccaaa aaccgccagc gtcgagctgg ggttcatatc
    15301 aataatactg ttgagtgaga cgtgtagcgc atcggcatca caatgcccct ggtgagcgag
    15361 ttgttgcacc aggcgtgccg actgacaaat accggccagg gcgagggtga tgtcatagta
    15421 attctttgcc acgttcactg cttccttgtt ttaagtaaag ataataatca gaccggcagc
    15481 ggcagacgct gctcaataat accgccaccg aggcacactt caccgttata gaagacggca
    15541 gactggcccg gcgtcacggc ggcaaccggc tcatcgaaaa tcactttaat gcgatcgtcg
    15601 tccagcgcct tgacggtgca cggaatgtcg gtctggcgat agcgggtttt taccgtgcaa
    15661 cgcatagtgc cggtgaatgg ttcgcgatcg acccagtgca gctgctgggc aatcaacccg
    15721 acagacatca gccgcgggtg ttcatggccc tgagcgacaa ccagaatgtt gttttcgacg
    15781 tctttgtcca ccacatacca cggttcttcg gtgccttctt tggtgccacc gatacccaga
    15841 cctttacgct gaccgagagt gtgatacatc agcccctggt gctcaccaat ttcatcgcca
    15901 tcgacggtaa tgattttgcc cggttgcgcc gggagataac ggcccaggaa ctcgcggaat
    15961 ttacgttcgc caatgaagca aatgccggta gagtcttttt tcttcgcggt gaccagacca
    16021 agatcttcag caatcttacg cacctgcggt ttttccagtt cgcctaccgg gaacaggctt
    16081 tgcgcaatct gctcatggct gagcgtataa aggaagtagc tctggtcttt attgctgtcc
    16141 agaccacgca gcaggcggct cttgccatcg acatcggccc gacgtacgta atgaccggta
    16201 gcgatataat cggcacctaa atcttcggcg gcaaattcga ggaaggcttt aaatttgatc
    16261 tccttgttgc acagaatatc cggattcggc gtgcgaccgg ctttatattc ggcaaggaac
    16321 agttcgaaga cgttgtccca gtactcggca gcaaagttaa cggtgtgcag ttcaatgccg
    16381 agcttgtcgc agaccgcctg ggcatcagcc agatccgctg ccgctgtgca atattcctca
    16441 ccgtcgtctt cttcccagtt cttcataaac aggccttcga cctgatatcc ctgttgttgc
    16501 aacagccagg cagaaacgga ggaatcgaca ccgccggaca tgccgacgat tacttttttt
    16561 gcggtttcag acattggatc actcacgaca ttgaacttca aggcggcgta ttctatcacg
    16621 cagcctttta gatgacaccc tttgtaaaag gccagttaaa atcgccaatc atctccagcg
    16681 gataacgttg cccgctttga taacaacgaa tactttccgc caccagcggc gaacgaagat
    16741 ttgacgcctg taaaatttct tcggcgctga cccaacggca gcagtcgata tcgctgtcat
    16801 gaggctgggt cgggcatatt tgctcaagct caatggcaaa gaggaaacgc aaaaacggcg
    16861 ttttatctgg cgcaatccac tgatgcatac gaataaagtg ttgcggctgc gcgctgatgc
    16921 cggtttcttc ccacagttca cgggcggcgg cttccactaa ggtttcatcg gcttccagat
    16981 gcccggcagg ttggttccat aacgctttac cattaatcgt ctcttcaacg actaaaaatt
    17041 tgccttctgc gtgcaccacg caagcaacgg taacgtgcgg tttaaacatt ttgcatcctt
    17101 aatctgtcac ttctcgccat tcgccattgg caagattatc caaagagtaa tctcccatcg
    17161 catagcgaat cagtcgcagc gtggggaaac caacatgggc ggtcatgcgg cgcacctggc
    17221 gattacgtcc ttcatataag gtgatcttca gccagctggt gggaatactt ttgcgttcac
    17281 gaattggtgg attccgcggc cataaccacg cgggttcgtc aaccagctcc gcgcctgcgg
    17341 gcagggtagg gccatcattt aaggttacgc cattgcgcaa ggcttcaagt gcgtcttgtg
    17401 tgggaatacc ttccacctgc acataataga tttttccggt gcgtttaccc ggctgggtta
    17461 aacgcgcctg caacgcgccg ttattggtca gcaccagcaa cccttcgcta tcgcggtcaa
    17521 ggcgacctgc tgcataaacg ccctgaaccg ggatgaattc ttttaatgtt ttgcgtccgg
    17581 tttcatcggt gaactgcgga agaacatcgt agggtttatt gaagaggatc acacgcgtgg
    17641 gctggttttc atgtttacgc ctggtagaac gttgtgagct gaatcgctta acctggtgat
    17701 ttctaaaaga agttttttgc atggtatttt cagagattat gaattgccgc attatagcct
    17761 aataacgcgc atctttcatg acggcaaaca atagggtagt attgacaagt caattacaaa
    17821 tcattaacaa aaaattgctc taaagcatcc gtatcgcagg acgcaaacgc atatgcaacg
    17881 tggtggcaga cgagcaaacc agtagcgctc gaaggagagg tgaatggaaa gtaaagtagt
    17941 tgttccggca caaggcaaga agatcaccct gcaaaacggc aaactcaacg ttcctgaaaa
    18001 tccgattatc ccttacattg aaggtgatgg aatcggtgta gatgtaaccc cagccatgct
    18061 gaaagtggtc gacgctgcag tcgagaaagc ctataaaggc gagcgtaaaa tctcctggat
    18121 ggaaatttac accggtgaaa aatccacaca ggtttatggt caggatgtct ggctgcctgc
    18181 tgaaaccctt gatctgattc gtgaatatcg cgttgccatt aaaggcccgc tgaccactcc
    18241 ggttggtggc ggtattcgtt ctctcaacgt tgccctgcgt caggaactgg atctctacat
    18301 ctgcctgcgt ccggtacgtt actatcaggg cactccaagc ccggttaaac accctgaact
    18361 gaccgatatg gttatcttcc gtgaaaactc ggaagacatt tatgcgggta tcgaatggaa
    18421 agctgactct gccgacgccg agaaagtgat taaattcctg cgtgaagaga tgggcgtgaa
    18481 gaaaattcgc ttcccggaac attgcggtat cggtattaag ccgtgttctg aagaaggcac
    18541 caaacgtctg gttcgtgcag cgatcgaata cgcaattgct aacgatcgtg actctgtgac
    18601 cctggtgcac aaaggcaaca tcatgaagtt caccgaaggc gcgtttaaag actggggcta
    18661 ccagctggcg cgtgaagaat ttggcggtga actgatcgac ggcggcccgt ggctgaaagt
    18721 taaaaacccg aataccggca aagagatcgt cattaaagac gtgattgctg atgcattcct
    18781 gcaacagatc ctgctgcgtc cggctgaata tgatgttatc gcctgtatga acctgaacgg
    18841 tgactacatt tctgacgctc tggcagcgca ggttggcggt atcggtatcg cccctggagc
    18901 aaacatcggt gacgaatgcg ccctgtttga agccacccac ggtactgcgc cgaaatacgc
    18961 cggtcaggac aaagtaaacc ctggctctat tattctctcc gctgagatga tgttacgcca
    19021 tatgggctgg actgaagccg cagacctgat tgttaaaggt atggaaggcg cgatcaacgc
    19081 caagaccgta acctatgact tcgagcgtct gatggaaggc gctaaactgc tgaaatgttc
    19141 agagtttggt gatgcgatca tcaagaatat gtaatcacta catgtgttaa atatttaaac
    19201 gggcgtataa cacgcccgtt gttttattta tgtggatatt attaatagca tatcgagcat
    19261 a
//
LOCUS       DAJVRP010000084        17413 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVRP010000084 DAJVRP010000000
VERSION     DAJVRP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.084"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            122..565
                     /locus_tag="OQN12_002950"
     CDS             122..565
                     /locus_tag="OQN12_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1655195.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            801..1619
                     /gene="ybhA"
                     /locus_tag="OQN12_002951"
     CDS             801..1619
                     /gene="ybhA"
                     /locus_tag="OQN12_002951"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCS1655196.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1620..2678)
                     /gene="modC"
                     /locus_tag="OQN12_002952"
     CDS             complement(1620..2678)
                     /gene="modC"
                     /locus_tag="OQN12_002952"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1655197.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2681..3370)
                     /gene="modB"
                     /locus_tag="OQN12_002953"
     CDS             complement(2681..3370)
                     /gene="modB"
                     /locus_tag="OQN12_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1655198.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3370..4143)
                     /gene="modA"
                     /locus_tag="OQN12_002954"
     CDS             complement(3370..4143)
                     /gene="modA"
                     /locus_tag="OQN12_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1655199.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4310..4459)
                     /gene="acrZ"
                     /locus_tag="OQN12_002955"
     CDS             complement(4310..4459)
                     /gene="acrZ"
                     /locus_tag="OQN12_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1655200.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4588..5376
                     /gene="modE"
                     /locus_tag="OQN12_002956"
     CDS             4588..5376
                     /gene="modE"
                     /locus_tag="OQN12_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1655201.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5444..6916
                     /gene="modF"
                     /locus_tag="OQN12_002957"
     CDS             5444..6916
                     /gene="modF"
                     /locus_tag="OQN12_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1655202.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7177..8193
                     /gene="galE"
                     /locus_tag="OQN12_002958"
     CDS             7177..8193
                     /gene="galE"
                     /locus_tag="OQN12_002958"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1655203.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8203..9249
                     /gene="galT"
                     /locus_tag="OQN12_002959"
     CDS             8203..9249
                     /gene="galT"
                     /locus_tag="OQN12_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1655204.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9253..10401
                     /gene="galK"
                     /locus_tag="OQN12_002960"
     CDS             9253..10401
                     /gene="galK"
                     /locus_tag="OQN12_002960"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1655205.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10395..11435
                     /gene="galM"
                     /locus_tag="OQN12_002961"
     CDS             10395..11435
                     /gene="galM"
                     /locus_tag="OQN12_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1655206.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11638..12390
                     /gene="gpmA"
                     /locus_tag="OQN12_002962"
     CDS             11638..12390
                     /gene="gpmA"
                     /locus_tag="OQN12_002962"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1655207.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12557..13609)
                     /gene="aroG"
                     /locus_tag="OQN12_002963"
     CDS             complement(12557..13609)
                     /gene="aroG"
                     /locus_tag="OQN12_002963"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1655208.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13925..14305
                     /locus_tag="OQN12_002964"
     CDS             13925..14305
                     /locus_tag="OQN12_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1655209.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14419..15360
                     /gene="zitB"
                     /locus_tag="OQN12_002965"
     CDS             14419..15360
                     /gene="zitB"
                     /locus_tag="OQN12_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1655210.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15357..16076)
                     /gene="pnuC"
                     /locus_tag="OQN12_002966"
     CDS             complement(15357..16076)
                     /gene="pnuC"
                     /locus_tag="OQN12_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1655211.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16114..17157)
                     /gene="nadA"
                     /locus_tag="OQN12_002967"
     CDS             complement(16114..17157)
                     /gene="nadA"
                     /locus_tag="OQN12_002967"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1655212.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4439 a         4545 c         4325 g         4104 t
ORIGIN      
        1 agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc gactgttaat
       61 tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt tcggcctttg
      121 catggatctt gcactggtaa caggacagcg caaaggagat ctggcggcta tgagatggga
      181 ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa aaattcgcat
      241 ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac tggataactt
      301 gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca ggacaattgc
      361 agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg acccaccgcc
      421 atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag gcagtgattt
      481 cactcaggcg ctccttggac atcgttcatc cagcatgacg gataaatacc gtgacggacg
      541 agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat gccacttgta
      601 attaattgat tataaagaat taattcctaa agagcatttg cttcatgaat gctcctttgc
      661 atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat gctgaattaa
      721 aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg aaaattctta
      781 accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta gacggcacct
      841 tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc cgcgctcgcg
      901 aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt catccttttt
      961 atcaggcgct ggcgctggat acacctgcta tttgctgtaa tggcacctat ttgtatgatt
     1021 atcatgcaaa aaccgtgctg gaagcggacc caatgcccgt taataaagcc ctgcaactca
     1081 ttgagatgct gaatgaacac cacattcacg gtctgatgta tgtcgatgat gcaatggtct
     1141 atgagcaccc gaccgggcat gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg
     1201 aacagcgtcc gactttcaca caagtcgctt ctctggctga aacgacgcaa caagttaacg
     1261 ccgtatggaa gttcgccctg acgcacgatg acctgccgca attgcagcat tttggtaagc
     1321 atgtcgaaca tgaactggga ctggagtgtg aatggtcctg gcacgatcag gttgatattg
     1381 cacacggcgg caacagcaaa ggtaaacgtt tgacgaaatg ggttgaggcg caaggttggt
     1441 cgatggaaaa cgtcgtggca ttcggcgata actttaatga tatcagtatg ctggaagccg
     1501 ctggtacagg cgtggcgatg ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg
     1561 tgattggtga taacaccacc gacagcattg cccagttcat ttatagccac ctgatttaat
     1621 caggcggtta tcgacacact tttaatttgc gcgtacagcc acaggccagg tttgatcgcc
     1681 agttcatccc tggcccacgg gctgatacgc gcccacagcg ttttaccgcc gacttccagc
     1741 tccacttcca cctggccgtt gtcgtcataa ctattaacaa cttttgcccg caatacgtta
     1801 cgaatgctgg tttgctgcgg cggttgtaaa accagagaaa catcggaagc ctgaatgcgg
     1861 atacgtagcg cagcttgcag cggttcgccc agcttattga cccacaaatg ctgatcgccc
     1921 agcgccagcg cggtcatcgc gtaatgcgga tgatgctcaa gcaccgtcac tttcagaatg
     1981 ctactttgtt gctctttcgg cagccacgga ttcatcacgc tactgcccca cacttcctcc
     2041 agcgcgccaa aggctttcac ctgaccgttt tccagtacca tcactctgtc tgccagatgg
     2101 aggatctcat ccagcgaatg gctgacatac aacatcggaa tgttgatttc ccgtgtcagc
     2161 cgttgcagat aaggcaacag ttcgcgttta cgcggaatat ccagtgacgc cagcggttca
     2221 tccagcaaca gcaattccgg tgctgtcagc aaagcccgac caatcgccac gcgctgtttt
     2281 tcgcctccgg acaggctgcc tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc
     2341 accagcttat cgaactgatc gaccatactt ttcgacatgc cgtagcgcag attgccacgc
     2401 actttgtaat gcgggaacaa ccgcgcatcc tgaaaaacat agccaacacg acgcttttcc
     2461 ggcgtcaggc agataccttt ttcggcatca tttagtaccc gcccattgag gacaatccgc
     2521 cctttttgcg ggcgcgtcag tccactgatg gcgttaatca gcgaagtttt tccggcaccg
     2581 gagacgccaa agatagcagt gatgccattg gcgggcagcg tttcattaat ggtcaggcaa
     2641 tggttgccca acgtctggga aaaattcagc tccagcatga ttagcgcccc gcccgttcac
     2701 ggctgattct ggccagccat tctgaaatca acagggagat catcgccagc gcaatagaaa
     2761 taatgcacag tctcgccgcg ccactttccc cgccgggggt ctggatcagg gtatacatgg
     2821 cagaaggaat ggttcgcgtt tcgctaggaa tgttcgacac aaaggtgatg gttgcaccaa
     2881 actcaccgag agaacgagca aaagccagta ccgtaccaac aataattccc ggtaaggtca
     2941 gcggtaacgt gatagtaaag aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct
     3001 gttccagttt gacatcaacc ccttccagcg ccagacgaat tgcccgcacc atcagcggaa
     3061 acgacatgac ggcggcagcg agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa
     3121 accagtcata cagacgttca ccgataaatc cgcgccgtcc catcgaaact aataataagt
     3181 aaccgacgac cacgggcggt aacaccagcg gtagatgcag tacgctgtcg agcagagctt
     3241 tgcccggaaa cgtgcaacgc accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa
     3301 acagcacagc cagggaagaa actttcaggc ttaataaaac tgcctgccat tctggatcgg
     3361 tcagtatcat tacttggttg taaatccgta acgtttaaag atttccgctg cctgcggtcc
     3421 cttcagataa tcataaaatg ctttcactgt cgcattgtta tgcccttcca caacagcaac
     3481 cggatattcc acttttttat gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt
     3541 gctggcaact gcgtcagaac cgtagacaat gcccagaggc gcttcgttac gttcgaccag
     3601 cgccagcgcc ccacgaacat cttccgctgg ggccagtttc ggagagagcg tatcccatgc
     3661 gcccagtttt tgcagtgctt cttttgcata aatgccagcg ggaacatgtt ccggatcgcc
     3721 aaccgccagg cgaccgccat tcagcagcga agtccagtta gttttgctgt cgatggtgaa
     3781 atctttctgc tcgctggctt tcggtgctac aacgaccagg ctattgccga gcagtgtctg
     3841 acgcgtagca gtatcgatcg cttttttatc aaccgcataa tccatccatt tctgatcggc
     3901 agaaataaac agatccgcag gggcacccgc ttcaatctga cgggcgagag tagatgacga
     3961 agcgaaagaa gaaaccacat ccacgccttt ctctttttta tactgcgtag caatgtcctg
     4021 cattgcgtta gtcagtgatg cggcggcgaa caccgtgatt ttcccttcat ctgccagtgc
     4081 attgccagca actgcgaaag agagtgccgc cccggcaaac aagttcaacc atttacgagc
     4141 cattggtaac cccttaatgt aacgttatgt aggcgacaat ataacgacta actcggaatt
     4201 ttcccagcag ttattgctaa cctttgtgag gtagataaga aaaaatatcg gcaggaaaag
     4261 caggaagttg agaaaaagaa aatgcccgac taagcgggca ctcagggaat caatgatttt
     4321 gtccgggctg gtctttttta ccaacaccag aaaagatgtt gaatacttca ccaagaccgt
     4381 aaatcagacc caggatgatg gccatcacga caggtaccat gattacggcg aataccagac
     4441 tttttaataa ctctaacatg gtcaactcca gatatagtca tgagactatt ctaaccgcta
     4501 agcacagaaa agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg ttttccgtca
     4561 caataagact tttgccagga cattgttatg caggccgaaa tccttctcac ccttaagctc
     4621 caacaaaaat tattcgccga cccgcgccgc atttcgctac taaaacacat tgcgctttcc
     4681 ggttccatta gccagggagc gaaagatgcc ggtatcagct ataaaagcgc ctgggatgcc
     4741 attaacgaga tgaatcagtt aagtgagcat attctggtcg agcgcgcaac aggcggtaaa
     4801 ggtggcggag gcgcagtact gacccgctat ggtcagcgac tgattcagct ctatgactta
     4861 ctggcgcaaa tccagcaaaa agcctttgat gtgttaagtg acgatgacgc cctgccgctg
     4921 aacagcctgc tggccgcgat ctcacgtttt tcactgcaaa ccagcgcccg taaccagtgg
     4981 ttcggtacca tcaccgcccg cgatcatgat gacgttcaac agcatgttga tgtcttactg
     5041 gctgacggaa aaacacgcct gaaagtcgca attaccgcac aaagcggcgc acgtctgggg
     5101 ctggatgaag gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg cattactcag
     5161 gacgaagcgg tcgcacaaaa cgccgacaac caattaccgg gtattattag tcatattgag
     5221 cgcggcgcag agcagtgcga agtattaatg gcgctacccg acgggcaaac actgtgcgcc
     5281 acagtgccgg taaatgaagc gacttctctt cagcaagggc agaatgtcac ggcctacttt
     5341 aatgccgaca gcgtgattat cgccacgctg tgctaagcgt gttgacaatt tgttatgaaa
     5401 cacgtatccc tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat cgttgcaaat
     5461 tttgcaaggc acgtttcgtc ttagcgacac aaaaacgctg caattgcctc agctaacgtt
     5521 aaacgcgggt gatagttggg cgtttgtcgg ttcgaatgga agtggaaagt cggcgctggc
     5581 ccgcgcgctg gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc agttttccca
     5641 catcactcgt ctctccttcg agcaattgca aaagctcgtc agcgacgaat ggcaacggaa
     5701 taacaccgat atgctcggcc ctagcgaaga ttacaccgga cgcactacgg ctgagattat
     5761 tcaggatgaa gtaaaggatg caccgcgttg catgcaactg gcgcagcagt tcggtattac
     5821 cgccctcctc gaccgacgct ttaaatacct ttccactggc gagacgcgaa aaaccctgct
     5881 gtgtcaggcg ctgatgtcgg agcctgactt gttgattctt gatgagccgt tcgatggcct
     5941 ggatgttgcc tcacgtcagc agctggctga gcgactcgcc tcgttacatc agtccggtat
     6001 tactttggta ctggtgctca atcgcttcga taagatcccg gaatttgtcc agtttgctgg
     6061 cgtgctggcg gattgtacgt tagcggaaac tggcgctaaa gaggaactgc tccagcaagc
     6121 actcgtcgcg caacttgcgc atagtgaaca gcttgaaggt gtgcaactgc cggagccgga
     6181 tgaaccttca gcacgtcacg ccttacccgc caacgaatcg cgcattgtgc tgaacaatgg
     6241 cgtggtttct tataatgatc gccccattct taataacctt agctggcagg tgaatccagg
     6301 cgaacactgg caaattgtcg ggccaaatgg cgcgggaaaa tcgacgttat taagcctgat
     6361 tactggcgat catccgcaag gttacagcaa cgatttgacg cttttcggcc gacgtcgcgg
     6421 cagcagcgaa accatctggg acatcaaaaa gcatatcggt tacgtcagca gtagtttgca
     6481 tctggattac cgggtcagca ctaccgtgcg taatgtgatt ctttctggct attttgattc
     6541 gattggcatt tatcaggtcg tttcggatcg ccagcaaaaa ctggtgcagc agtggctgga
     6601 tattctcggc attgataaac acacggctga cgctccgttc catagtcttt cctggggaca
     6661 gcagcgtctg gcgctgatcg tccgcgcact ggtgaaacat ccgacgttgc ttattctcga
     6721 tgaaccacta caggggcttg atccgctcaa tcgccaactt atccgccgtt ttgttgatgt
     6781 gctgattagc gaaggtgaaa cgcaattgtt gtttgtttcg caccacgctg aagatgcgcc
     6841 tgcctgtatt acccatcgcc ttgagttcgt gccggacggt ggactctatc gctatgtgct
     6901 gacaaaaata tattgagtcg gtagtgctga ccttgccgga ggcggcctaa gcgcactctc
     6961 cggccaacgg ttcgacgcat gcaggcatga aaccgcttct tttttcagat aaaaagcgca
     7021 atcattcata aaccctctgt tttataatca cttaatcgcg cataaaaaac ggctaaattc
     7081 ttgtgtaaac gattccacta atttattcca tgtcacactt ttcgcatctt tgttatgcta
     7141 tggttatttc ataccacaag cctaatggag cgaattatga gagttctggt taccggtggt
     7201 agcggttaca ttggaagtca tacctgcgtg caattactgc aaaacggtca tgatgtcatc
     7261 attcttgata acctctgtaa cagtaagcgc agcgtactgc ctgttatcga gcgtttaggc
     7321 ggcaaacatc caacgtttgt tgaaggcgat attcgtaacg aagcgttgat gaccgagatc
     7381 ctgcacgatc acgctatcga caccgtgatc cacttcgccg ggctgaaagc cgtcgccgaa
     7441 tcggtacaaa aaccgctgga atattacgac aacaatgtca acggcactct gcgcctgatt
     7501 agcgccatgc gcgccgctaa cgtcaaaaac tttattttta gctcctccgc caccgtttat
     7561 ggcgatcagc cccaaattcc atacgttgaa agcttcccga ccggcacacc gcaaagccct
     7621 tatggcaaaa gcaaattgat ggtggaacag atcctcaccg acctgcaaaa agcccagccg
     7681 gactggagca ttgccctgct gcgctacttc aacccggttg gcgcgcatcc gtcgggcgat
     7741 atgggcgaag atccgcaagg cattccgaat aacctgatgc catacatcgc ccaagttgct
     7801 gtaggccgtc gcgactcgct ggcgattttt ggtaacgatt atccgaccga agatggtact
     7861 ggcgtacgcg attacatcca cgtaatggat ctggcggacg gtcacgtcgt ggcgatggaa
     7921 aaactggcga acaagccagg cgtacacatc tacaacctcg gtgctggcgt aggcagcagc
     7981 gtgctggacg tggttaatgc cttcagcaaa gcctgcggca aaccggttaa ctatcatttt
     8041 gcaccgcgtc gcgaaggcga ccttccggcc tactgggcgg acgccagcaa agccgaccgt
     8101 gaactgaact ggcgcgtaac gcgcacactc gatgaaatgg cgcaggacac ctggcactgg
     8161 cagtcacgcc atccacaggg atatcccgat taaggaacga ccatgacgca atttaatctc
     8221 gttgatcatc cacatcgccg ctacaacccg ctcaccgggc aatggattct ggtttcaccg
     8281 caccgcgcta agcgcccctg gcagggggcg caggaaacgc cagccaaaca ggtgttacct
     8341 gcgcacgatc cagattgctt cctctgcgca ggtaatgtgc gggtgacagg cgataaaaac
     8401 cccgattaca ccgggactta cgttttcact aatgactttg cggccttgat gtctgacacg
     8461 ccagatgcgc cagaaagcaa cgatcctttg atgcgttgcc agagcgcgcg cggtaccagc
     8521 cgggtgatct gcttttcacc ggatcacagt aaaacgttgc cagaactgag cgttgcggca
     8581 ttgacggaaa tcgtcaaaac ctggcaggag caaaccgcag agctgggaaa aacgtacccg
     8641 tgggtgcagg tctttgaaaa caaaggtgcg gcgatgggct gctctaaccc gcatccgcac
     8701 ggacagattt gggcaaatag cttcctgcct aacgaagctg agcgcgaaga ccgcctgcaa
     8761 aaagaatatt ttgccgaaca gaaatcacca atgctggtgg attatgttca gcgcgagctg
     8821 gcagacggta gccgtaccgt tgtcgaaacc gaacactggt tagccgttgt gccttactgg
     8881 gctgcctggc cgttcgaaac gctactgctg cccaaagccc acgttttgcg gatcaccgat
     8941 ttgaccgacg cccagcgcag cgatttggcg ctggcgttga aaaagctgac cagtcgttat
     9001 gacaacctct tccagtgctc cttcccctac tctatgggct ggcacggcgc accatttaat
     9061 ggcgaagaga atcaacactg gcagctgcac gcgcactttt atccgcctct gctgcgctcc
     9121 gccaccgtac gtaaatttat ggttggttat gaaatgctgg cagagaccca gcgagacctg
     9181 accgcagaac aggcagcaga gcgtttgcgc gcagtcagcg atatccattt tcgcgaatcc
     9241 ggagtgtaag aaatgagtct gaaagaaaaa acacaatctc tgtttgccaa cgcatttggc
     9301 taccctgcca ctcacaccat tcaggcgcct ggccgcgtga atttgattgg tgaacacacc
     9361 gactacaacg acggtttcgt tctgccctgc gcgattgatt atcaaaccgt gatcagctgt
     9421 gcaccacgcg atgaccgtaa agttcgcgtg atggcagccg attatgaaaa tcagctcgac
     9481 gagttttccc tcaatgcgcc cattgtcgcg catgaaaact atcaatgggc gaactacgtt
     9541 cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct tcggcggtgt ggacatggtg
     9601 atcagcggca atgtgccgca gggtgccggg ttaagttctt ccgcttcact ggaagtcgcg
     9661 gtcggaaccg tattgcagca gctttatcat ctgccgctgg acggcgcaca aatcgcgctt
     9721 aacggtcagg aagcagaaaa ccagtttgtt ggctgtaact gcgggatcat ggatcagcta
     9781 atttccgcac tcggcaagaa agatcatgcc ttgctgattg actgccgctc actggggact
     9841 aaagcagttt ccatgccgaa aggtgtggct gtcgtcatca tcaacagtaa cttcaaacgt
     9901 accctggttg gcagcgaata caacacccgt cgtgaacagt gcgaaaccgg tgcgcgtttc
     9961 ttccagcagc cagctctgcg cgatgtcacc attgaagagt tcaacgctgt tgcacatgag
    10021 ctggacccaa tcgtggcgaa acgcgtgcgt catatcctga ctgaaaacgc ccgcaccgtt
    10081 gaagctgcca gcgcgctgga gcaaggcgac ctgaaacgta tgggcgagtt gatggccgag
    10141 tctcatgcct ctatgcgcga tgatttcgaa atcaccgtgc cgcaaattga cactctggta
    10201 gaaatcgtca aagctgtgat tggcgacaaa ggtggcgtac gcatgaccgg cggcggattt
    10261 ggcggctgta tcgtcgcgct gatcccggaa gagctggtgc ctgccgtaca gcaagctgtc
    10321 gctgaacaat atgaagcaaa aacaggtatt aaagagactt tttacgtttg taaaccatca
    10381 caaggagcag gacagtgctg aacgaaactc ccgcactggc acccgatggt cagccgtacc
    10441 gactgttaac tttgcgtaac aacgcaggga tggtagtcac gctgatggac tggggtgcga
    10501 ctttactttc cgcccgtatt ccgctttccg atggcagcgt ccgcgaggcg ctgctcggct
    10561 gtgccagtcc ggaatgctat caggatcagg ctgcgtttct gggggcctct attggtcgtt
    10621 atgccaaccg catcgccaat agtcgctata cctttgacgg tgaaaccgtg acgctttcgc
    10681 caagccaggg cgttaaccag ctgcacggcg gcccggaagg gttcgacaaa cgtcgctggc
    10741 agattgtgaa ccagaacgat cgtcaggtgc tgtttgccct gagttcagat gatggcgatc
    10801 agggcttccc gggtaatctc ggcgcgacgg tgcaatatcg tctgaccgac gataaccgta
    10861 tctccattac ttatcgcgcc acagttgata aaccttgccc ggtgaatatg actaatcacg
    10921 tctatttcaa tcttgacggc gagcagtctg acgtgcgcaa tcacaagttg cagattctgg
    10981 cggacgaata tcttccggtt gatgaaggcg gcattccgca cgacggcttg aaatctgtcg
    11041 ccggaacgtc ttttgatttc cgcagcgcca aaatcatcgc cagtgagttt cttgccgacg
    11101 acgatcagcg caaagtgaaa ggttacgatc acgcattctt gttacaggcc aaaggcgatg
    11161 gcaagaaagt ggcggcgcat gtctggtcag cagatgaaaa attgcagctg aaggtctaca
    11221 ccaccgctcc ggctcttcaa ttctactccg gcaacttcct cggcggcaca ccgtcgcggg
    11281 gaaccgaacc ttacgccgac tggcaagggc tggcgttgga aagcgagttt ctgccggaca
    11341 gcccgaacca ccctgaatgg ccgcaaccgg actgcttcct gcgtcctggc gaagagtatt
    11401 ccagcctgac ggaatatcag tttattgctg agtaatgtta agccctcgtc atgagggctt
    11461 tatcttcata ttgttcaaat caccagcaaa caccgacata tttgcaactc aatattcaca
    11521 acaaccttac actgcgccac tattttcgct atggttatgc gtaagcattg ctgttgcttc
    11581 gtcacggcaa tataatgaga attattatca ttaaaagatg atttgaggag taagcatatg
    11641 gctgtaacta agctggttct ggttcgtcat ggcgaaagtc agtggaacaa agaaaaccgt
    11701 ttcaccggtt ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc aaaagcagca
    11761 ggtaagctgc tgaaagagga aggttacagc tttgactttg cttacacttc tgtgctgaaa
    11821 cgcgctatcc ataccctgtg gaatgtgctg gacgaactgg atcaggcatg gctgcccgtt
    11881 gagaaatcct ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct gaacaaagcg
    11941 gaaactgctg aaaagtatgg cgacgagcag gtgaaacagt ggcgtcgtgg ttttgcagtg
    12001 actccgccgg aactgactaa agatgatgag cgttatccgg gtcacgatcc gcgttacgcg
    12061 aaactgagcg agaaagaatt gccgctgacg gaaagcctgg cgctgaccat tgaccgcgtg
    12121 atcccttact ggaatgaaac cattctgccg cgtatgaaga gcggtgagcg cgtaatcatc
    12181 gccgcacacg gtaactccct gcgtgcgctg gtgaaatatc ttgataacat gagcgaagaa
    12241 gagattcttg agctgaatat cccgactggc gtgccgctgg tgtatgagtt cgacgagaat
    12301 ttcaaaccgc tgaaacgcta ttatctgggt aatgctgacg agatcgcggc gaaagcagca
    12361 gccgttgcga accagggtaa agcgaagtaa acgtcattcg tttaaaaaat gagaaagccg
    12421 actgcaagtg agtcggcttt tttgtttgtc gttgctaaca atggagcaca ttgcctgatg
    12481 cgacgctgcg cgtcttatca ggcctgtggt gattcatcgg atacgccact ctgacggcgc
    12541 atccgacaat taaaccttac ccgcgacgcg cttttactgc attcaccagt tgacgtaaca
    12601 gagcatcggt atcttcccag ccaatgcagg catcggtgat gctcttaccg taggccagcg
    12661 gctccccgct atcgaggctc tgattgcctt ccaccagatg gctttccacc atcacgccaa
    12721 taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca tccatctgct
    12781 ttttgaattg tttggacgaa ttagcatggc tgaaatctat catcacctga gctggcagac
    12841 ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag ttaggctctt
    12901 taccgccgcg cagaatgata tggcaatcgc cgttaccact ggtattcaca atcgccgaat
    12961 gcccccattt agttacggac aggaagcagt gcggcgcacc ggcggcatta atggcatcga
    13021 tagccacttt aatcgtaccg tctgtgccat ttttaaaacc taccggacaa gaaagaccag
    13081 acgccagttc gcggtgcacc tgcgattccg tggtacgtgc accaattgcg ccccagctca
    13141 tcaggtcagc gagatactga ggagtgatca tatcgagaaa ctcacccgcc gctggcagac
    13201 cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg ttgatctgga
    13261 agctgttatc catatgcgga tcgttaatca accctttcca gcccaccgtg gtacgcggct
    13321 tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca cgcagcgcca
    13381 gcaagcgagt ggcatactct ttagccgcga caggatcatg aattgagcac gggccaatca
    13441 ccaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt cgggcatggg
    13501 caaccgtatt cgcggcattt tcagttgcgg ggaatttttc cagcaatgcg acaggaggaa
    13561 gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg tctgttccag
    13621 tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc gtcagaatgt
    13681 gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa aaacgccaga
    13741 ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt gtttgttaat
    13801 tatttcaaga ttctctgctg cgtttcataa actggctgaa atcttaaacc aatgccttat
    13861 attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat aacaggagca
    13921 tcctatgaaa atgacaaaac tggccacatt atttctgact gccactctaa gccttgccag
    13981 cggtgccgca ctggctgccg atagcggagc gcaaactaat aacggccagg caaacgccgc
    14041 agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa ataacgtcga
    14101 caataacggg gtaaataccg gttctggcgg cacaatgctg cattcggatg gttcttcaat
    14161 gaacaatgac ggaatgacca aagatgaaga gcacaagaac accatgtgca aagatggtcg
    14221 atgtccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg atgtcgatac
    14281 caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga gagcggagtc
    14341 tctccctttt aatttttcac ttctaaaagc agtatgatgt cataaagtta agtgagaata
    14401 attaagggat gactcgtcat ggcgcactca cactcacaca cgtcctcaca cctgccagaa
    14461 gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt tatgctggta
    14521 gaagtcgttg gtggttttct ttctggttct ctggcattgc tggccgatgc gggtcatatg
    14581 ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc acgtcgtcct
    14641 cccaccattc gccacacttt cggttggcta agactgacca cgctcgcggc gtttgtgaat
    14701 gccatcgccc tcgtggtaat taccattttg attgtctggg aggcgataga acgcttccgc
    14761 acgccgcgtc cggtcgaggg aggcatgatg atggcaattg ccgtggctgg gttgctggca
    14821 aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct caacgtacga
    14881 gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat tattgccgcg
    14941 ttgattatta tctggactgg ctggacacct gctgacccca ttctctcgat actggtgtcg
    15001 cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga attacttgaa
    15061 ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg ggaaatcccg
    15121 gaagtccgca atgtgcacca tgtacatgta tggatggtag gcgagaagcc ggtgatgacg
    15181 ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca gatccaacac
    15241 tacctgatgg atcactatca gattgagcac accaccattc agatggaata tcaaccttgt
    15301 cataggccag actgccacct gaatgaaggg gtgtctggcc attcacatca tcaccattaa
    15361 tgggacagcg cgcgtgagcc tctttcacgt gcgctgttga tccacatccg gctgccgttg
    15421 agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc ctgaagtgca
    15481 aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc cacatactta
    15541 cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc ccagaacggg
    15601 aaagcgtccg gttgcagttc aggcatcaca acctgtaatc ctaatgcttg catgatcatg
    15661 actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat cagaccaatc
    15721 gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca gcgaattttc
    15781 aactccgcct cgttctgact ggtttgtcgc gaccacgcat accagccgta aatattcgcg
    15841 gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa gaaaataatg
    15901 ccaaacaagg taacgttgat caggccaaag aagtagttgc tgatcttctc cagactggca
    15961 aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga gagatcataa
    16021 ccgcccgccc ctattggtat atgtaccagg atattctgca cactaaaaaa atccatcttt
    16081 cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag ccgcaaaatc
    16141 cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctctcatcga catgaacctc
    16201 gtgattactt ccttcctgtt ctaatgcctc tgcgatagcc tgaaggtcat tcatggccat
    16261 ccacggacaa tgcgcgcagc tgcggcaggt tgcgccctca cccgcggttg gtgcttccag
    16321 taactcttta tctggcaccg cctgctgcat tttgtagaaa ataccccgat cggttgccac
    16381 aataagcctc tgatgaggca atgcttttgc agcagcgatc agttgactgg tggaaccgac
    16441 cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta tggcagcatc
    16501 cgggtattct tcttgcaagc gggttaacgc ctgagtctta aattcatcat gcacaatgca
    16561 ggcaccctgc cagcataaaa tgtctccacc cgtctgtttt tgcacgtaac gccccagatg
    16621 tttgtcgggt gcccagatga ttttttcacc caaactatca agatgatcaa taagttcgac
    16681 ggcaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag aagtgttggc
    16741 gtagacgacg acagtacgat cgggatgggc atcgtaaaat gcgttaaatt cttcaacagg
    16801 gcagccgaga tccagtgaac actcaacctg aagtgtcggc atcagaattg ttttttccgg
    16861 actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca aagtagaagc
    16921 gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc caccggtttc
    16981 ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca ccgcattacg
    17041 ttcttttagc aaacgtttta tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg
    17101 cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatta cgctcatctt
    17161 accatctcgt tttacaggct taacgttaaa accgacatta gcgtaacatt cgctgttatc
    17221 ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa
    17281 ttacgcctaa ttccaccgac gtagagttgc ttgcacagta agctctgacg atgtcatcag
    17341 gtttacaaag tgaaattgag gatgcctgaa tagcaaaaaa gcgcctttag ggcgcttttt
    17401 tacattggtg ggt
//
LOCUS       DAJVRP010000085        17385 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVRP010000085 DAJVRP010000000
VERSION     DAJVRP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.085"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(42..773)
                     /gene="rlmB"
                     /locus_tag="OQN12_002968"
     CDS             complement(42..773)
                     /gene="rlmB"
                     /locus_tag="OQN12_002968"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1655213.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(953..3394)
                     /gene="rnr"
                     /locus_tag="OQN12_002969"
     CDS             complement(953..3394)
                     /gene="rnr"
                     /locus_tag="OQN12_002969"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1655214.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="OQN12_002970"
     CDS             complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="OQN12_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1655215.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4063..5361)
                     /gene="purA"
                     /locus_tag="OQN12_002971"
     CDS             complement(4063..5361)
                     /gene="purA"
                     /locus_tag="OQN12_002971"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1655216.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5465..5662)
                     /locus_tag="OQN12_002972"
     CDS             complement(5465..5662)
                     /locus_tag="OQN12_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1655217.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="OQN12_002973"
     CDS             complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="OQN12_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1655218.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="OQN12_002974"
     CDS             complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="OQN12_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1655219.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="OQN12_002975"
     CDS             complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="OQN12_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1655220.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRACTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="OQN12_002976"
     CDS             complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="OQN12_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1655221.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="OQN12_002977"
     CDS             complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="OQN12_002977"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1655222.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="OQN12_002978"
     CDS             complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="OQN12_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1655223.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="OQN12_002979"
     CDS             complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="OQN12_002979"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1655224.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="OQN12_002980"
     CDS             complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="OQN12_002980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1655225.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="OQN12_002981"
     CDS             complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="OQN12_002981"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1655226.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15981..17120
                     /gene="queG"
                     /locus_tag="OQN12_002982"
     CDS             15981..17120
                     /gene="queG"
                     /locus_tag="OQN12_002982"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1655227.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17103..17156)
                     /locus_tag="OQN12_002983"
     CDS             complement(17103..17156)
                     /locus_tag="OQN12_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655228.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3942 a         4959 c         4367 g         4117 t
ORIGIN      
        1 aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg ctgacgaacc
       61 gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc
      121 attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct
      181 tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag tgtatgatcc
      241 gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc
      301 gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc tacttttttc
      361 gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg aacgcctgcg
      421 gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc gtcgaggatc
      481 agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc ctgatactga
      541 cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg
      601 agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc
      661 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
      721 gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta
      781 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat
      841 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
      901 caaagcgcat tttgtcagca atctaaccct ctttttttaa agagggtatt gatcactctg
      961 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
     1021 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
     1081 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga cgacgcttgc
     1141 cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
     1201 gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg
     1261 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca
     1321 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
     1381 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
     1441 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
     1501 cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1561 gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat
     1621 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
     1681 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
     1741 tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag catggtttgc agcatttctg
     1801 catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt
     1861 taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
     1921 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     1981 gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac
     2041 gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     2101 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
     2161 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     2221 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     2281 cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     2341 acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca
     2401 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     2461 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     2521 cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat
     2581 caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct tcttccggca
     2641 cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata tacggaattt
     2701 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2761 cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga
     2821 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
     2881 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     2941 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     3001 cagcacccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     3061 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     3121 gaccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     3181 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     3241 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     3301 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     3361 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     3421 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     3481 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3541 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3601 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
     3661 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3721 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3781 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3841 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3901 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     3961 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     4021 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
     4081 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     4141 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
     4201 tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     4261 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     4321 agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     4381 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     4441 acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     4501 agtttcatca aacagttcgg ccgggaaagg acctgcacct acacgagttg agtaagcttt
     4561 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4621 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4681 cgtaccctgc gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4741 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4801 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4861 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4921 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     4981 gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agagcaacgt gataatcaag
     5041 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     5101 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     5161 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     5221 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     5281 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     5341 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     5401 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     5461 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     5521 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5581 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5641 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5701 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5761 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5821 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
     5881 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcataa
     5941 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
     6001 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     6061 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     6121 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     6181 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg
     6241 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     6301 cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtccagg cgaccaattt
     6361 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     6421 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     6481 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6541 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag
     6601 gctcataaac caaaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6661 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6721 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6781 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6841 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6901 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     6961 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     7021 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     7081 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     7141 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggtata
     7201 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     7261 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     7321 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     7381 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     7441 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     7501 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7561 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7621 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7681 gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7741 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7801 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7861 tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7921 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     7981 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     8041 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     8101 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     8161 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     8221 gctgataaaa acgactcctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     8281 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     8341 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     8401 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     8461 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     8521 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8581 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8641 caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8701 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8761 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8821 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8881 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     8941 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     9001 gctcaacctg caacttaccc tcatgggtac acgcacgttg ggcgaaaata tctaaaataa
     9061 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     9121 ggctcagggc atggtcaaaa agaacgaccg aagcacccat cgctttgaca gcttccgcaa
     9181 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     9241 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     9301 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     9361 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     9421 ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     9481 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9541 tgttactgtg atgagaaacc gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa
     9601 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
     9661 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9721 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9781 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9841 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
     9901 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
     9961 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    10021 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    10081 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    10141 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    10201 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    10261 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    10321 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
    10381 tctgccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
    10441 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    10501 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10561 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10621 gcgttcggct tcgccgtccc gataaccatc cctttgtaaa taagggcaga atcaacgctt
    10681 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10741 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10801 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10861 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10921 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    10981 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    11041 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    11101 aatacccaat tccgctaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    11161 aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt
    11221 caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc
    11281 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    11341 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    11401 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    11461 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    11521 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11581 cgggatggca cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg
    11641 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11701 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11761 cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg
    11821 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11881 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc
    11941 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc
    12001 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    12061 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    12121 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    12181 gtagaacaga tccagcacct ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt
    12241 cacgtccatg tcgcgtcctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    12301 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    12361 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    12421 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    12481 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12541 ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12601 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12661 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    12721 catcggatgc gcaaggaagt aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    12781 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12841 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    12901 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    12961 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    13021 agactgactg ttcgccagca catcacccgc cccacccagc agctccgaca gtttctcgta
    13081 ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca cccatacgga
    13141 agcgccagtc gcactacggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    13201 gttttgctta tgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    13261 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    13321 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    13381 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    13441 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    13501 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13561 tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac
    13621 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag
    13681 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac
    13741 cagattattg ccgctgaaca acaacggcag tccctgaatc acgcccgttt gtttgatatc
    13801 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    13861 caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc
    13921 acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat
    13981 catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga
    14041 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    14101 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    14161 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    14221 caaatcaaag tgatagacca ttaagttgtc gagcgtatag ggttcgacca gcgtataagt
    14281 ggggcttttg acattaccct gatgacccag agcctgtaaa aagccccggc taaaggtggt
    14341 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    14401 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    14461 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    14521 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg
    14581 cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    14641 taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa
    14701 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    14761 cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag
    14821 cgacggaaca gcctaacaac cgtgcggcct cgctaggatg cggcgtgatc acgcgattgt
    14881 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    14941 tgcgaaaatt ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg
    15001 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt
    15061 catgcaccat caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca
    15121 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    15181 ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag
    15241 tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    15301 tctcctgacc tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa
    15361 catcccgcgc ttttccagtg agcaagccta gtttcagcgc aataaaagtg atggtgtgat
    15421 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    15481 caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    15541 cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt
    15601 cgggccagac aatattcgaa gcatggatct cgccacccgc gtttaaccat gcttcgcgtg
    15661 ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac gtgacctcaa
    15721 tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    15781 cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    15841 cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcacatct gccgcctcgc
    15901 gttctccgcg gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt
    15961 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    16021 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    16081 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    16141 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattaccc ggtacgctgc
    16201 gtgtaatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    16261 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    16321 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    16381 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc
    16441 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt
    16501 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    16561 gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    16621 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact tgaaggggcg ctcccggaag
    16681 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    16741 cgtggaatcg ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    16801 acgcaccgga actcattgag ttatttgcct ggagcgaaga gaagttttta aaagtcacgg
    16861 aaggatcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    16921 taggcaatgc cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc
    16981 acccacttct cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg
    17041 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag
    17101 ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    17161 attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacggaatct tgatttttaa
    17221 cttatttatc aataggttaa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga
    17281 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc
    17341 agcaataacg catcgcgtcg ctgtagatat tttattgaga gaaga
//
LOCUS       DAJVRP010000086        17042 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVRP010000086 DAJVRP010000000
VERSION     DAJVRP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.086"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..214)
                     /locus_tag="OQN12_002984"
     CDS             complement(<1..214)
                     /locus_tag="OQN12_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCS1655229.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            524..2296
                     /gene="aspS"
                     /locus_tag="OQN12_002985"
     CDS             524..2296
                     /gene="aspS"
                     /locus_tag="OQN12_002985"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1655230.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2414..2866
                     /gene="nudB"
                     /locus_tag="OQN12_002986"
     CDS             2414..2866
                     /gene="nudB"
                     /locus_tag="OQN12_002986"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1655231.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2895..3635
                     /locus_tag="OQN12_002987"
     CDS             2895..3635
                     /locus_tag="OQN12_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1655232.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3670..4191
                     /gene="ruvC"
                     /locus_tag="OQN12_002988"
     CDS             3670..4191
                     /gene="ruvC"
                     /locus_tag="OQN12_002988"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1655233.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4193..4795)
                     /locus_tag="OQN12_002989"
     CDS             complement(4193..4795)
                     /locus_tag="OQN12_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCS1655234.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="OQN12_002990"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="OQN12_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS1655235.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="OQN12_002991"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="OQN12_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS1655236.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="OQN12_002992"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="OQN12_002992"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1655237.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OQN12_002993"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="OQN12_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1655238.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OQN12_002994"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="OQN12_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1655239.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="OQN12_002995"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="OQN12_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1655240.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="OQN12_002996"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="OQN12_002996"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1655241.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="OQN12_002997"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="OQN12_002997"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1655242.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OQN12_002998"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="OQN12_002998"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1655243.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OQN12_002999"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="OQN12_002999"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1655244.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="OQN12_003000"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="OQN12_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1655245.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..>17042
                     /locus_tag="OQN12_003001"
     CDS             16439..>17042
                     /locus_tag="OQN12_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1655246.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGK"
BASE COUNT         4350 a         4203 c         4430 g         4059 t
ORIGIN      
        1 catcaaccgg ctgttttaat gcttcggcgt aatcggcaga ccagccaact cgcaccagaa
       61 acacgggctg accgctggcg cgaaatttcg ccgccagctt cccggcgcga ttaactacct
      121 catcggcagt atgtggacca ccggcaaaag gtaagatgcc ttcttgtaaa tcgatcacca
      181 ccaacgcagt ggttttagca ttaagttcaa gcatgctgac tcctgaaaaa acaaaatcgt
      241 gcctcacacc ttaatgcgat tagccgatgg cggaacgaca aattttgtta atttttatga
      301 gttcgggcaa cacctttata gcaaactggc gtagagtctg atttcaggcc cgatataaag
      361 tggtaacgag gcgttccagc gcgggccagg ctcttacggt gcggcagaat ttccagtata
      421 atagccgcct tttttcattc agttgtgaca tacagctaac gctgcgactt ggtcacctgc
      481 gcagcagggg gcatccgccg gcagctaagt taagggatat ctcatgcgta cagaatattg
      541 tggacagctc cgtttgtccc acgtggggca gcaggtgact ctgtgtggtt gggtcaaccg
      601 tcgtcgtgat cttggtagcc tgatcttcat cgatatgcgc gaccgcgaag gtatcgtgca
      661 ggtatttttc gatccggatc gtgcggacgc gttaaagctg gcctctgaac tgcgtaatga
      721 gttctgcatt caggttacgg gcaccgtacg tgcgcgtgac gaaaaaaata ttaaccgcga
      781 tatggcgacc ggcgaaattg aagtgctggc gtcctcgctg actatcatca accgcgcaga
      841 tgttctgccg cttgactcta accacgtcaa caccgaagaa gcgcgtctga aataccgcta
      901 cctcgacctg cgtcgcccgg aaatggcgca gcgcctgaaa acccgcgcta aaatcaccag
      961 cctggtgcgc cgttttatgg atgaccacgg cttcctcgac atcgaaactc cgatgctgac
     1021 caaagccacg ccggaaggcg cgcgtgacta cctggtgcct tctcgtgtgc acaaaggtaa
     1081 attctacgca ctgccgcaat ccccacagtt gttcaaacag ctgctgatga tgtccggttt
     1141 tgaccgttac tatcagatcg ttaaatgctt ccgtgacgaa gacctgcgcg ctgatcgtca
     1201 gcctgaattt actcagatcg atgtggaaac ttctttcatg accgcgccgc aagtgcgtga
     1261 agtgatggaa gcgctggtgc gtcatttgtg gctggaagtg aagggcgtgg atctgggcga
     1321 tttcccggta atgacctttg cggaagcaga acgccgttat ggttctgata aaccggatct
     1381 gcgtaacccg atggaactga ccgacgttgc cgatctgctg aaatctgttg agtttgcagt
     1441 atttgcgggg ccggcgaacg atccgaaagg ccgtgttgcc gctctacgtg ttccgggtgg
     1501 cgcatcgctg acccgtaagc agatcgacga atacggtaac ttcgttaaaa tctacggcgc
     1561 gaaaggtctg gcttacatca aagttaacga acgcgcgaaa ggtctggaag gtatcaacag
     1621 cccggtagcg aagttcctta atgcagaaat catcgaagcc atcctggatc gtactgccgc
     1681 gcaagatggc gatatgattt tcttcggtgc cgacaacaag aaaattgttg ccgacgggat
     1741 gggcgcactg ctcctgaaag tgggtaaaga ccttggtctg accgacgaaa gcaaatgggc
     1801 accgctgtgg gttatcgact tcccgatgtt tgaagacgac ggtgaaggcg gcctgacggc
     1861 aatgcaccat ccgttcacct caccgaaaga tatgactgct gcagaactga aagctgcacc
     1921 ggaaaatgcg gtggcgaacg cttacgatat ggtcatcaat ggttacgaag tgggcggtgg
     1981 ttcagtacgt atccataatg gtgatatgca gcagacggtg tttggtattc tgggtatcaa
     2041 cgaagaggaa cagcgcgaga aattcggctt cctgctcgac gccctgaaat acggtactcc
     2101 gccgcacgca ggtctggcat tcggtcttga ccgtctgacc atgctgctga ctggcaccga
     2161 caatatccgt gacgttatcg ccttcccgaa aaccacggcg gcagcgtgtc tgatgactga
     2221 agcaccgagc tttgctaacc cgactgcact ggctgagctg agcattcagg ttgtgaagaa
     2281 ggctgagaat aactgatatg actcaaatac acgaaatcat tcgcgttgca tcaaggcggc
     2341 aactgagtga actcccatga gcatagataa ctatgtgaat gggatgagcg aaggcagtca
     2401 acgaagaggc agcgtgaagg ataaagtgta taagcgtccc gtttcgatct tagtggtcat
     2461 ctatgcacaa gatacgaaac gggtgctgat gttgcagcgg cgtgacgatc ccgatttctg
     2521 gcagtcggta accggcagcg tggaagaggg tgaaaccgcg ccgcaagctg ccatgcgcga
     2581 agtaaaggaa gaggttacca ttgatgttgt cgctgaacaa ctgaccttaa ttgactgtca
     2641 gcgcacggta gagtttgaaa ttttttcaca tttacgtcat cgctatgcgc cgggcgtgac
     2701 gcgtaatacg gaatcatggt tctgtcttgc gcttccgcac gagcggcaga tcgttttcac
     2761 tgaacatctg gcttacaagt ggcttgatgc gcctgctgcg gcggcgctca ctaagtcctg
     2821 gagcaaccgg caggcgattg aacagtttgt aattaacgct gcctgaacag gcagggctat
     2881 ctggagatat ttttatggca ggtcatagta aatgggccaa caccagacat cgtaaagctg
     2941 cgcaggatgc taagcgcggt aaaatcttca ctaaaatcat tcgtgagctg gtaaccgcgg
     3001 ctaagctggg cggtggcgat ccggacgcta acccgcgtct gcgtgcggcg gtggataaag
     3061 cactgtctaa caacatgacc cgtgacacac tgaaccgcgc aattgcacgt ggtgtgggcg
     3121 gtgatgatga tgcaaacatg gaaaccatca tctacgaagg ttacggtcct ggcggcacgg
     3181 caatcatgat tgaatgtctg tctgacaacc gcaaccgtac cgttgctgaa gtgcgtcacg
     3241 cattcagcaa atgtggcggt aacctcggca ctgacggttc cgtagcttat ctgttcagca
     3301 aaaaaggcgt gatctccttc gagaaaggcg acgaagacac catcatggaa gcagctctgg
     3361 aagcaggtgc tgaagacgtt gtgacatatg atgacggcgc gattgatgtc tacactgcat
     3421 gggaagaaat gggtaaagtg cgcgacgctc tggaagcggc aggtctgaaa gcagacagcg
     3481 cggaagtttc catgatcccg tccaccaaag ctgatatgga tgcagaaacc gcgccgaaac
     3541 tgatgcgtct gatcgatatg ctggaagatt gcgacgacgt gcaggaagtt taccataacg
     3601 gtgaaatctc tgatgaggtt gcagcgactc tctgatgatg cctgctaaac agcaaaacgg
     3661 agacgcgtga tggctattat tctcggcatt gacccgggtt cgcgcgtgac cggctacggc
     3721 gtcatccgcc aggtaggtag gcaactgtcc tacctgggta gcggatgcat acgcaccaaa
     3781 gtggatgatt taccgtctcg tctgaagctc atctatgcgg gcgtgacgga aatcatcacc
     3841 cagttccagc ctgattattt cgccattgaa caagtcttta tggcgaagaa cgctgactca
     3901 gccctgaaac tgggccaggc gcgcggcgtg gcgattgttg cggcggtgaa tcaggaattg
     3961 ccggtatttg aatacgcggc acgtcaggta aagcaaacgg tggtaggtat gggtagtgcc
     4021 gaaaaaagcc aggtgcagca tatggtccgc accttgctga aactgcccgc taatccacag
     4081 gcggatgccg ccgatgcgct ggcgattgcc atcacccact gccacgttag tcagaatgcg
     4141 atgcagatga gcgaatcgcg gctgaacctg acgagagggc gactgcgtta agttataccg
     4201 cctcggtcag ttccggctga ggcgtttcaa ctccctccgc gtgaatcaac accaaacccg
     4261 gatgatgcca caaactggct ggcgtgacgg ttttacgctc ccacggaata gaacctaaga
     4321 accagaattt ccagaaagtg agttttgcat tcggattgct atttaacagt tgtccaaacg
     4381 tctctatttc acccaccgga atacatagcg cctctttata aatatcaaag acaaattttg
     4441 aacagaactg gcgcgaagat tcgtatttaa aacccgtgtg gtaaagtttg cgtagccggg
     4501 agggaacctg ttcaacaatt cgttgttttt gttgttctgt cagcccagcg tctaatcgct
     4561 ttatagcata gcgttgatta gacgaacgtt taataaaacg agatagcgtg gtgatggttg
     4621 aaaggggaac ccggctttct gcaaccagga agtcttcacc gttatgaccg ataatgatcc
     4681 cgacgtgatt actccagcaa tttgatgcag ctgatatttg accaaataag gcagcaccta
     4741 tacatgtaaa gacgatatct ccaatttcat attcagcagg gtaattaata ttcacgttgt
     4801 cattccattg aaatagatac gaatatacgt aatagcataa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccttggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagtgcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag caggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcactttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaagacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 ag
//
LOCUS       DAJVRP010000087        16881 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVRP010000087 DAJVRP010000000
VERSION     DAJVRP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.087"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..176
                     /locus_tag="OQN12_003002"
     CDS             <1..176
                     /locus_tag="OQN12_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCS1655247.1"
                     /translation="KDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIG
                     NLHYIDTGAVFGG"
     gene            complement(201..392)
                     /locus_tag="OQN12_003003"
     CDS             complement(201..392)
                     /locus_tag="OQN12_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCS1655248.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(497..733)
                     /locus_tag="OQN12_003004"
     CDS             complement(497..733)
                     /locus_tag="OQN12_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCS1655249.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(851..2290)
                     /gene="rsmF"
                     /locus_tag="OQN12_003005"
     CDS             complement(851..2290)
                     /gene="rsmF"
                     /locus_tag="OQN12_003005"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS1655250.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2370..4990)
                     /gene="yebT"
                     /locus_tag="OQN12_003006"
                     /pseudo
     CDS             complement(2370..4990)
                     /gene="yebT"
                     /locus_tag="OQN12_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4959..6242)
                     /gene="yebS"
                     /locus_tag="OQN12_003007"
     CDS             complement(4959..6242)
                     /gene="yebS"
                     /locus_tag="OQN12_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS1655251.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6372..6869
                     /gene="msrC"
                     /locus_tag="OQN12_003008"
     CDS             6372..6869
                     /gene="msrC"
                     /locus_tag="OQN12_003008"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1655252.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6966..7664
                     /gene="proQ"
                     /locus_tag="OQN12_003009"
     CDS             6966..7664
                     /gene="proQ"
                     /locus_tag="OQN12_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1655253.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7684..9732
                     /gene="prc"
                     /locus_tag="OQN12_003010"
     CDS             7684..9732
                     /gene="prc"
                     /locus_tag="OQN12_003010"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1655254.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9924..10805
                     /gene="htpX"
                     /locus_tag="OQN12_003011"
     CDS             9924..10805
                     /gene="htpX"
                     /locus_tag="OQN12_003011"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1655255.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10851..12224)
                     /locus_tag="OQN12_003012"
     CDS             complement(10851..12224)
                     /locus_tag="OQN12_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1655256.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12401..13192
                     /gene="kdgR"
                     /locus_tag="OQN12_003013"
     CDS             12401..13192
                     /gene="kdgR"
                     /locus_tag="OQN12_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1655257.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13335..13574)
                     /locus_tag="OQN12_003014"
     CDS             complement(13335..13574)
                     /locus_tag="OQN12_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655258.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13733..13876
                     /gene="mgrB"
                     /locus_tag="OQN12_003015"
     CDS             13733..13876
                     /gene="mgrB"
                     /locus_tag="OQN12_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1655259.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13951..14238
                     /locus_tag="OQN12_003016"
     CDS             13951..14238
                     /locus_tag="OQN12_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1655260.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14817..14960
                     /locus_tag="OQN12_003017"
     CDS             14817..14960
                     /locus_tag="OQN12_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1655261.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14973..15182
                     /gene="cspE"
                     /locus_tag="OQN12_003018"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14973..15182
                     /gene="cspE"
                     /locus_tag="OQN12_003018"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1655262.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15348..16157
                     /gene="rlmA"
                     /locus_tag="OQN12_003019"
     CDS             15348..16157
                     /gene="rlmA"
                     /locus_tag="OQN12_003019"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1655263.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16154..16720)
                     /gene="mntP"
                     /locus_tag="OQN12_003020"
     CDS             complement(16154..16720)
                     /gene="mntP"
                     /locus_tag="OQN12_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1655264.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4426 a         4114 c         4288 g         4053 t
ORIGIN      
        1 aaaaggacgt tgatttgcat caggtcttgt ggagccgctc gcgattaggt gaacgcccaa
       61 aagggcaggg aattacaggt gctgatcatt tctggtttgg tcatacaccg ttgcgacatc
      121 gcgtggatat tggcaacctg cattatattg ataccggtgc tgtctttggg ggctaactga
      181 ctcttgtgca actgcaataa ttaaaaatca ccatactcct gtgctggtcg ccagaaacca
      241 tctataaaat cctcaatcgg aaaacaaccg ccatggcgga tccgttgatc gctcatagaa
      301 taaagacact gctgttccgt gttgtagaca tccacaacaa tatcttcaca accgccatct
      361 aggtagcaaa caaaaagtac cagcgcgaac atttcatccc cgaagtgtgg tgccgtaccg
      421 ttaagtttag gagagatttt acaacggggg aataaccagg acaaataacc cgccatgccg
      481 acgggttctt tttggatcag gcaagacgca taatccaggc atcagattgt tgatcgtaac
      541 gggtacggta aaggacagag tgttcgccat ttagcagggc agggatactg gtttcagcat
      601 cccaggcatc cagttgcata cataaatcgg ggtcagattt acaaggaatg gttaacgttc
      661 gatcacccgg cgattcaccg tgtaattcgc cgtcgtcgat ttcaaaagcg cctatgcgca
      721 cactggtttt catcttcgtg ctctccattt agctgctgat tgtagtacga ccagtccgat
      781 cgcacaattt ttcagcctag ttgaggccgg cgaatgtgcc agtcaaaaag tgcgcttttt
      841 ttgtgcgctg tcatgcgtta ctggtaaaaa gtttcccgtc ccgcaccagt tcacgcgggt
      901 agctgttttt cagtcgcgaa ccaatccgtt tggctaaacc aatcggctga tgctggaaag
      961 taaccaacac gtcatccgcc actggcgcgg cttgcgggta aacatcgcgc ccgcgatacc
     1021 actcctccgc ttcctgcggt gtcagttcaa aagcgtttac attgtcgggg gaggcatggg
     1081 caataaccgc ttcatgctgc cagcgataac ctttgttatg cgtttcggca agtttaatcc
     1141 ctaagcgaga aaatcggact ttaccgatca gggcttcaat gcctaccggg aacaaccaca
     1201 gttctttgtc gcgttgccag agtcgcaggt ttccatccca gtttaagcca acacttgcag
     1261 ccgcctgacg aatttgtccg gcttcgcgat ctttcaccgg gctgaacggg aaattgccca
     1321 ctttgtattt tggggcgggt aaggcgggga tcgcctgagt tttacgcaga cgagcaacga
     1381 agaagccttc gcagtcgtaa atttgtggga aaacatgcaa aaaaccttct tcggtcagcg
     1441 ccttgtttgc accagggaag agatcgccaa gtggtaaaaa ctctacttcg tcggggtaag
     1501 tctctttcag ccaccggcaa acggcttcgt tttcttcccg gtttaaggta caggttgagt
     1561 aaaccagcgt accgccagga cgtaatgcat gaaaggcgct gtcaatcagt tcccgttggg
     1621 tagctgcgat ttcctgattg ctttcgggtg accagttttt tagcgcatcg ggatctttac
     1681 gcaccacgcc ttcgccggag cagggagcgt ccagcaaaat ggcatcaaac atttctggca
     1741 ctgccgcacc aaacacgcgg ccaccaaaat gtgtgagcgc aacattactg atgccacagc
     1801 ggctgatatt ggcatgtaac acttttaccc aactggcgga aaactcattg gcaaggattg
     1861 ccccttcgtt attcatccgc gcggcaattt gcgtcgtttt tgagcctggc gcagcagcga
     1921 catccatcac ccgctgtggt gcattaccgt cagcaaacaa ggcggcgacg ggcaacattg
     1981 aactggcttc ctgaatataa aacaggccac ttaaatgctc ggtggtacta cccaatggca
     2041 atgcatcttc attgtcgcgt tcaatccaga aaccttcttc acaccacgga attggcgtaa
     2101 gcgtccagcc ataaggagcg gttaattgca ggaaatcagc aacggagatt ttcagcgtat
     2161 taacgcgaat gctgcggcgc aacgggcgct gacaggcggc aagaaaatca tcaaatgaga
     2221 gcgtcgaagg catagcttcg cgcatttgtg tcagaaacgc gtccgggaaa taaacggtgt
     2281 gttgggccac gagcatgtac cactggcaaa aaaacaggcg cgcagtttag cataagcgct
     2341 ccggcgcagg cacgccggag cagtgggcat tatttgggaa gcgcagttcc ccattcacgc
     2401 cactctttcg gttcactttc ctgcaacagg aagtgtttgc cttcttgggc tttcggtgca
     2461 agtggcgtac ccggaggcgt ggcgaaggcg atgccgccac ggataaattg gttaaaggtg
     2521 ccggttttca ctacgccgcc cgtcagacca aagtccagac tgtaacccga tgccaaccag
     2581 aagacggaat tgttacgcac caggtgttga tagcgtttac tgatgcgcat cgcaatcatc
     2641 acgcgatctg acaatgtccc cagcgtcatt ccggtaaccg tacctacttc cagaccacgg
     2701 aacagcacag gcgtaccgat gcctaacgaa ccggcttccg gcgcttcaac aataatgctt
     2761 aaaccatcta ggtaacgcga atcagtaatg gtggcctctt ctaattcaaa gtcgcgacga
     2821 ggattgccac ggcctggttc gacgttgata tacggctgga ggatagtatc tagatgctca
     2881 acgccagctg ccgagatttg cggtgtgacc actgagaagc gcgtaccacc gcgagcaaag
     2941 gtctggacat attccggata gagcaccgcc tttgcctgta cttcattgcg tgcggtaatc
     3001 agatccagcg tctggatttg cccgatatca ataccgagat agcgaattgg catcccgacc
     3061 gccagttttc cggcatcgaa agcgtgaagc gtaatctgcc tgccaaccgc acgggccgct
     3121 gtttcggaag catacagaat tcgcttgtcg cctttacgct gactggcgct ggcaccactg
     3181 aggttatcga agctaatggc cccctttaat gctctggaga gcggggatgc ctgtacggtc
     3241 aggccactac cattcagctg aactttcgcc ccgccttctg cccagaacac gctgttgctg
     3301 gtcaaaaggt tgcgatattc cggtttaatg tgcagatcga tatcgaacgc gttagctcgc
     3361 ggacgcacgg taataacttc accaacttca aatttacggt acagcactac cgatcctgcc
     3421 tgcacatccg gcagcgtctt tgcactcaaa ctcacggttg tggtgggtaa atcgctaagg
     3481 ctgttctcca gcgctttttc cagattggca tacagtggat agctggcttt catctcgcct
     3541 ttatcgcccg gcagaatacg tatcccgccg ttaatccatt ctgaggcgct ggcaccgaga
     3601 aactcaacac catccagccc caccttcacg tcgacacggc tgttgacgac aaatttgcta
     3661 tcgcctttta ccagttctcg atgctgaggc tcgatggcga cggtaaaggt gacgcctttg
     3721 ctggtgagtt tacgatcgat aacctggcct acctgcacgc cgtgaagaat gagcggctga
     3781 cccgcatcaa taccgtaact ttccggtgct gtcagggtca gtgtcagaac gtccggctct
     3841 tgcagcaggg ctttttcgcc cggcacaaca acgaactctt tgcgtggctc gccatcgccg
     3901 ggcaccaact caaaggtttt tccggtcagc agggcgctga gattagcatc gctaagggat
     3961 aatttcgggt tgcgtaattc gatgcgggta ttttcacgaa gcagggatca acggtcattt
     4021 ccccagtgac tttaccacca ggatttaaat ccagtttagt cagctgtccg acttccagcc
     4081 cctgatacat taacggcgtc gagtcggcgg ttaatcctgc tccactcggc agttccagtt
     4141 ttattattac tccacgctgg ctgtgggcca gatcttcata cagaccaaag gtatcttccg
     4201 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     4261 ccagactttc cagtttcacc ttcgcgccac tgatactgac gttggcatca acgccggaaa
     4321 cgtttcagaa acggctacct tttttcacca gatcggtaaa acgccgctcg atcaggacat
     4381 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccaccggga
     4441 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagagcgggg gcttgcaggt
     4501 ggatcatcag atcgccattg tccagccgat atttcggttg ggtatcgagc gcgacaaagt
     4561 gatcctgctc tttaccttta cccggcatca tgccgatata gttcccaccg acgagggcgt
     4621 ccagcccgga gacacctgcc aacgatgctt ttggcgtcat cagccagaac tgagtctctt
     4681 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4741 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4801 tacggcccgg aacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4861 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4921 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4981 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     5041 aaaattacgg cagcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     5101 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     5161 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     5221 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     5281 ctggcgataa acacgattcc cgcaaccgca atgttgctac ttgccagcga cataattccg
     5341 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     5401 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     5461 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agagaccacg ctgatcagga
     5521 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     5581 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5641 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5701 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5761 tctttaagtc gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5821 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5881 accattgatc ccgttatcgc atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5941 ttggcgtcaa tacgaatgcc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     6001 ggcataagca acagcatggt gaaggccatt gccgccaggc gcgttagcga ccagtcgcgc
     6061 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tttgatgaga atttatctcg
     6121 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     6181 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     6241 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     6301 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaccaa
     6361 gtgattaagt aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     6421 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     6481 agcgtctcac tgacgttaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     6541 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6601 gcacagcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6661 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6721 agattattgg tgttctcgac atcgatagta ccgtcttcgc tcgctttaca gacgaggacg
     6781 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6841 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6901 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6961 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     7021 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa attggtattt
     7081 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     7141 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     7201 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     7261 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     7321 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcaccg cgccgcgaac
     7381 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     7441 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     7501 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     7561 acgcgatgga tgccaccgta ttagaaatca ccaaagacgg cgtccgcgtc cagctgaatt
     7621 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7681 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7741 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7801 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7861 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7921 tacagccaca atgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7981 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     8041 caaaagcgcc gttttgaacg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     8101 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     8161 gctgagttga acgcgcagtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     8221 acaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     8281 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     8341 cgtgaaatcg atccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     8401 gaaatgagtt tgtcactgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     8461 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     8521 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgatgtgat tggctggcgt
     8581 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8641 ttacctgctg gtaaagggac caagacccgt actgtaacgt tgacccgtga acgtattcgt
     8701 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8761 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8821 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8881 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8941 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     9001 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     9061 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     9121 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     9181 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     9241 caacgtaaag gcgtaacacc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     9301 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca tcgatgccgc gacttatgtg
     9361 aaatcaggag atttaactgc ctttgaaccg gagctgctga aggaacataa tgcgcgtatc
     9421 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgaag
     9481 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     9541 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gagaaggtaa accggagttg
     9601 aagaagctgg atgatctatc gaaagattac caggagccgg atccttatct ggatgagacg
     9661 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9721 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9781 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9841 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9901 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9961 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
    10021 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
    10081 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
    10141 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggcg gggatcgcta
    10201 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
    10261 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
    10321 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
    10381 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
    10441 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
    10501 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
    10561 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10621 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10681 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10741 tcatgactca cccgccgctg gataaacgta ttgaagctct gcgtacgggt gaatacctga
    10801 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccctg
    10861 gctcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10921 gcagccatca gcgagacgtg cgtaccatta tcaccaaact gatttagcat cagcgccacc
    10981 agcgccgcgc cactactctg acccagtaga cgagccgttc ctaacatgcc actggcccca
    11041 ccgctacgtt cgcgcggagc ggaggtaata atggtgtggt tattgggtga ctggaataag
    11101 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    11161 ggcagcagaa ccagggaaaa aagccccgta gccatgatga acaaccctaa agcacccagc
    11221 aatcctgcat gtacgcgttc aatcaaatag cctgccagcg gagccatcac catcgttgct
    11281 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    11341 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    11401 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    11461 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    11521 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    11581 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11641 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11701 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11761 gcagcagaag agacggcaac aataaacgag tttatgccca tccctctccc cagaaaacgt
    11821 tgtggataga tcaggcggat aagtgcggta ttaacgctca tcaacgctgc accgccgaaa
    11881 ccttgtatga cacgcgcaag ggtgagcatt tgcagcgaat cagaaagggc gcagaacagt
    11941 gaagacaaca gaaaaacgac cagaccgcat ttataaatac gtcgatagcc aaacatatcg
    12001 cccagaaacg aaaacgagag cagggagatg acaatggcga tttgataggc gttcactacc
    12061 cagatggaac tggctggtgt ggcatgaagg tccgtggcaa ttgttggcag ggcgacgttg
    12121 gcgattgcgc cgtcaaggac agccatcgaa ataccaatca caatggttaa tatcgcaccg
    12181 tatcgctggg gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt
    12241 tggtgaataa tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa
    12301 ttgtaactaa ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa
    12361 cagttctgat ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga
    12421 taaacagcct gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg
    12481 tgaagagcgc gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac
    12541 cgtttatcgc tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc
    12601 ggagaaatat tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt
    12661 cgatttaatt cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac
    12721 tatccacctc ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat
    12781 gtacaatttg cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat
    12841 tggtaaggta ctgctggcat ggcgcgatcg cgatgaagtg aagcaaattc ttgagggcgt
    12901 ggagtataaa cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct
    12961 ggaccaggtg cgcgagcagg ggtatggaga agataacgaa gagcaggaag aagggctgca
    13021 atgcattgcg gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc
    13081 cttcccgacg ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac
    13141 cgcagcgcgc aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta
    13201 agaacctgtc ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg
    13261 aaggtgcgtt atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg
    13321 gttatttatt acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa
    13381 tctgttatct ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg
    13441 gcagtcagat aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa
    13501 acgccatatc cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga
    13561 atgatgaatc gcattacaat ctcttctctt tttatgttcg cttaatcgta gcggcaatat
    13621 gcgctgaagc aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt
    13681 taactaacgc atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa
    13741 gtttcgatgg gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt
    13801 attcaacatg atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca
    13861 gtttatcccg tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt
    13921 agaatagcgc ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg
    13981 aacattgcgt ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt
    14041 tttatcaatc gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg
    14101 gatcagcaaa aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag
    14161 aaagcagata aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc
    14221 ctggtcgccg aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact
    14281 caattcatcc tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc
    14341 tgacttcaaa taattatcat attatttatt agcgaaacgt ttctctcttg atttttttgc
    14401 tcactcatca atttttctta ttttaaattt acaatccctt tggggattga cttctcttta
    14461 gggtaattga tagccgttaa ctgactgttt tatgagaaaa agtgatataa ctttttattc
    14521 attgcatagc agaaaatgtg atattgcacg cactatgtaa taacttctcc ctctggcctg
    14581 gaacaactga acttattgaa ctatgttaga aactacgcca gtttaagtat ctgcctgaac
    14641 tggcaaggtt aagcacaatg atatatcggc gcgtattccg ttgcataagt gtgcaaaaaa
    14701 agtggaagac gtatcgagat ttgtgcgtct gatcgagaca tgtttaaaaa tggcttgcca
    14761 taattaacgt tgtatgtgat aaaagatttc gggttaaacg aggtacagtt ctgtttatgt
    14821 gtggcatttt cagtaaagaa gtcctgagta aacacgttga cgttgaatac cgcttctctg
    14881 ccgagcctta tattggtgcc tcatgcagta atgtgtcagt tttatctatg ttatgcctgc
    14941 gggcgaagaa aacaatctaa ggaatttttc aaatggcaaa gattaaaggt caggttaagt
    15001 ggttcaacga gtctaaaggt tttggcttca ttactccggc tgatggcagc aaagatgtgt
    15061 tcgtacactt ctccgctatc cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg
    15121 ttgagttcga aattcaggac ggccagaaag gtccggcagc tgttaacgta acagctatct
    15181 gatcgaatcc actgatctga agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga
    15241 atgcgaggct ttttactatg ctttatcttc gctcctggcg ttcggatatt tgcccgccgc
    15301 gtgattcgcg ttacacttgc ggcctttagt atcctgctgg agttgtcatg tcgttttcct
    15361 gtccactttg ccatcagcct ctttcgcgtg aaaaaaacag ctatatctgt ccccaacgac
    15421 atcagtttga tatggcgaaa gaagggtatg tcaatctgct gcccgttcag cataaacggt
    15481 ctcgtgatcc gggcgacagc gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg
    15541 gacattatca gccgctgcgt gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa
    15601 aggccacggc ggtgctggat attggctgtg gtgaagggta ttacacacac gcatttgccg
    15661 atgcgttgcc cgaaatcacc acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg
    15721 cggcgaaacg ctatccgcag gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt
    15781 ccgataccag tatggacgcc ataatacgta tttacgcgcc gtgtaaagca gaagaattag
    15841 cacgagtagt gaagcccggc ggctgggtca ttactgccac gccgggaccg cgacatttga
    15901 tggagctgaa ggggctgatt tacaatgaag tacatcttca tgcacctcat gcagaacaac
    15961 tggaaggttt tacattacag cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg
    16021 atgaagccgt cgcattattg cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct
    16081 ggcaaacact ggcagcaaaa gaagtgttcg actgccagac ggactttaat attcacctct
    16141 ggcagcgttc ttattaaccg tggaagtgcg tccagaggat ctggacgccg atgccgatca
    16201 gcaccagccc gccgagaatt tccgcttttt tcccaataat tgagccgata aagcgaccaa
    16261 ccatcatccc taatgtcgac ataatcaagg ttgcacaacc aatggccaat gcggtcgtga
    16321 taatgttgac ctgaaggaaa gcaagaccaa cacccacagc catggcatcc aggctggtgg
    16381 caatcgcggt ggttaccagt agccagaaac cgtgccgacg gcgcggctct tcatcttcat
    16441 catctgcgcc acgaaaaccc tcaataatca ttcgcccgcc gaggaatatc agcagcacaa
    16501 acgcaatcca gtgattccat tcaaggacaa accggctggc taacatgccc attccccagc
    16561 cgatcagcgg cgtcagggtt tcgacggcac caaaaataag gccggttcgc aatgcttcag
    16621 aaaatttcgg tttatggagg ggggcacctt taccgattga tgcagcaaat gcatccatcg
    16681 acataccaaa cgcaagaaga acagtagcag tgatattcat gacaacgtcc tgaccggggt
    16741 atccataaaa cacatcatcg cccccagcaa acatgcatac atgaacaaca agccgcgatg
    16801 ggttttcgcg ttcatgcagg catagcgatt gatgtgcata tggtctcgcc tgactgaaag
    16861 tcttcagtcc gtacgtgcca c
//
LOCUS       DAJVRP010000088        16389 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVRP010000088 DAJVRP010000000
VERSION     DAJVRP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.088"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OQN12_003021"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OQN12_003021"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1655265.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OQN12_003022"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OQN12_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1655266.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OQN12_003023"
     CDS             2339..2554
                     /locus_tag="OQN12_003023"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655267.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OQN12_003024"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OQN12_003024"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1655268.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="OQN12_003025"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="OQN12_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1655269.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="OQN12_003026"
     CDS             5920..6417
                     /locus_tag="OQN12_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1655270.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="OQN12_003027"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="OQN12_003027"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1655271.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="OQN12_003028"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="OQN12_003028"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1655272.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="OQN12_003029"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="OQN12_003029"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1655273.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="OQN12_003030"
     CDS             9102..9665
                     /locus_tag="OQN12_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1655274.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="OQN12_003031"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="OQN12_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1655275.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="OQN12_003032"
     CDS             complement(10257..11237)
                     /locus_tag="OQN12_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1655276.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="OQN12_003033"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="OQN12_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1655277.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="OQN12_003034"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="OQN12_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1655278.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="OQN12_003035"
     CDS             12540..12830
                     /locus_tag="OQN12_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1655279.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="OQN12_003036"
     CDS             12838..13431
                     /locus_tag="OQN12_003036"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1655280.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="OQN12_003037"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="OQN12_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1655281.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="OQN12_003038"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="OQN12_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="OQN12_003039"
     CDS             15882..16385
                     /locus_tag="OQN12_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCS1655282.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4059 a         4220 c         4127 g         3983 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccaa gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc agggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc ggccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccacctgc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgcc
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgggct
    12061 cattgtgatt tttacaaccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttaca gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccgcgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgga
//
LOCUS       DAJVRP010000089        15584 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVRP010000089 DAJVRP010000000
VERSION     DAJVRP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.089"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..73
                     /locus_tag="OQN12_003040"
     CDS             <1..73
                     /locus_tag="OQN12_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655283.1"
                     /translation="GLRFAIREGGRTVGAGVVAKVLG"
     gene            256..450
                     /gene="bfd"
                     /locus_tag="OQN12_003041"
     CDS             256..450
                     /gene="bfd"
                     /locus_tag="OQN12_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCS1655284.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            523..999
                     /gene="bfr"
                     /locus_tag="OQN12_003042"
     CDS             523..999
                     /gene="bfr"
                     /locus_tag="OQN12_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCS1655285.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(996..1463)
                     /locus_tag="OQN12_003043"
     CDS             complement(996..1463)
                     /locus_tag="OQN12_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1655286.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1844..2155
                     /gene="rpsJ"
                     /locus_tag="OQN12_003044"
     CDS             1844..2155
                     /gene="rpsJ"
                     /locus_tag="OQN12_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1655287.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2188..2817
                     /gene="rplC"
                     /locus_tag="OQN12_003045"
     CDS             2188..2817
                     /gene="rplC"
                     /locus_tag="OQN12_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1655288.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2828..3433
                     /gene="rplD"
                     /locus_tag="OQN12_003046"
     CDS             2828..3433
                     /gene="rplD"
                     /locus_tag="OQN12_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1655289.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3430..3732
                     /gene="rplW"
                     /locus_tag="OQN12_003047"
     CDS             3430..3732
                     /gene="rplW"
                     /locus_tag="OQN12_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1655290.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3750..4571
                     /gene="rplB"
                     /locus_tag="OQN12_003048"
     CDS             3750..4571
                     /gene="rplB"
                     /locus_tag="OQN12_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1655291.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4588..4866
                     /gene="rpsS"
                     /locus_tag="OQN12_003049"
     CDS             4588..4866
                     /gene="rpsS"
                     /locus_tag="OQN12_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1655292.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4881..5213
                     /gene="rplV"
                     /locus_tag="OQN12_003050"
     CDS             4881..5213
                     /gene="rplV"
                     /locus_tag="OQN12_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1655293.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5231..5932
                     /gene="rpsC"
                     /locus_tag="OQN12_003051"
     CDS             5231..5932
                     /gene="rpsC"
                     /locus_tag="OQN12_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1655294.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5945..6355
                     /gene="rplP"
                     /locus_tag="OQN12_003052"
     CDS             5945..6355
                     /gene="rplP"
                     /locus_tag="OQN12_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1655295.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6355..6546
                     /gene="rpmC"
                     /locus_tag="OQN12_003053"
     CDS             6355..6546
                     /gene="rpmC"
                     /locus_tag="OQN12_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1655296.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6546..6800
                     /gene="rpsQ"
                     /locus_tag="OQN12_003054"
     CDS             6546..6800
                     /gene="rpsQ"
                     /locus_tag="OQN12_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1655297.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6965..7336
                     /gene="rplN"
                     /locus_tag="OQN12_003055"
     CDS             6965..7336
                     /gene="rplN"
                     /locus_tag="OQN12_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1655298.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7347..7661
                     /gene="rplX"
                     /locus_tag="OQN12_003056"
     CDS             7347..7661
                     /gene="rplX"
                     /locus_tag="OQN12_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1655299.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7676..8215
                     /gene="rplE"
                     /locus_tag="OQN12_003057"
     CDS             7676..8215
                     /gene="rplE"
                     /locus_tag="OQN12_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1655300.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8230..8535
                     /gene="rpsN"
                     /locus_tag="OQN12_003058"
     CDS             8230..8535
                     /gene="rpsN"
                     /locus_tag="OQN12_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1655301.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8569..8961
                     /gene="rpsH"
                     /locus_tag="OQN12_003059"
     CDS             8569..8961
                     /gene="rpsH"
                     /locus_tag="OQN12_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1655302.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8974..9507
                     /gene="rplF"
                     /locus_tag="OQN12_003060"
     CDS             8974..9507
                     /gene="rplF"
                     /locus_tag="OQN12_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1655303.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9517..9870
                     /gene="rplR"
                     /locus_tag="OQN12_003061"
     CDS             9517..9870
                     /gene="rplR"
                     /locus_tag="OQN12_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1655304.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9885..10388
                     /gene="rpsE"
                     /locus_tag="OQN12_003062"
     CDS             9885..10388
                     /gene="rpsE"
                     /locus_tag="OQN12_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1655305.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10392..10571
                     /gene="rpmD"
                     /locus_tag="OQN12_003063"
     CDS             10392..10571
                     /gene="rpmD"
                     /locus_tag="OQN12_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1655306.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10575..11009
                     /gene="rplO"
                     /locus_tag="OQN12_003064"
     CDS             10575..11009
                     /gene="rplO"
                     /locus_tag="OQN12_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1655307.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11017..12348
                     /gene="secY"
                     /locus_tag="OQN12_003065"
     CDS             11017..12348
                     /gene="secY"
                     /locus_tag="OQN12_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1655308.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12380..12496
                     /gene="rpmJ"
                     /locus_tag="OQN12_003066"
     CDS             12380..12496
                     /gene="rpmJ"
                     /locus_tag="OQN12_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1655309.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12643..12999
                     /gene="rpsM"
                     /locus_tag="OQN12_003067"
     CDS             12643..12999
                     /gene="rpsM"
                     /locus_tag="OQN12_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1655310.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13016..13405
                     /gene="rpsK"
                     /locus_tag="OQN12_003068"
     CDS             13016..13405
                     /gene="rpsK"
                     /locus_tag="OQN12_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1655311.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13439..14059
                     /gene="rpsD"
                     /locus_tag="OQN12_003069"
     CDS             13439..14059
                     /gene="rpsD"
                     /locus_tag="OQN12_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1655312.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14085..15074
                     /gene="rpoA"
                     /locus_tag="OQN12_003070"
     CDS             14085..15074
                     /gene="rpoA"
                     /locus_tag="OQN12_003070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1655313.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15115..15498
                     /gene="rplQ"
                     /locus_tag="OQN12_003071"
     CDS             15115..15498
                     /gene="rplQ"
                     /locus_tag="OQN12_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS1655314.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4013 a         3660 c         4151 g         3760 t
ORIGIN      
        1 cggtctgcgt ttcgcaatcc gtgaaggcgg ccgtaccgtt ggcgcgggcg ttgttgctaa
       61 agttctgggc taattacacg ttaattagtt ttgaattgaa aagggcgctt cggcgccctt
      121 tttgctgccc gtaatttgag ctcgaaatat ttagtcgtaa ttttgctcgc tgaactatgg
      181 cgcaaataaa actgtactat tgtaatcata accattctca tttacacttt gtgcagaatt
      241 tgaacgggag caatcatgta cgtttgtttg tgtaatggtg taagtgacaa aaaaattcgc
      301 caggcagtcc gtcagtttca tccgcaatct ttccaacaat tgcgtaagtt tattcctgtg
      361 ggaaatcaat gcggtaagtg cattcgcgcc gcgcgcgaag tgatgcagga cgagttgatg
      421 cagatgccag aatttaaaga gattgcctga agcccacgct tattttttga catcctgcta
      481 gccccatcta cgctctaata gtggaagcgg agggactata aaatgaaagg tgatgttaaa
      541 atcataaatt atctcaataa actattggga aatgagcttg tcgcaatcaa tcagtatttt
      601 ctccatgccc ggatgtttaa aaactggggc ctgatgcgtc tcaatgacgt cgaataccat
      661 gaatccatcg atgagatgaa acacgctgat aaatatatcg agcgtatttt gtttttagaa
      721 ggaatcccca acttacagga tttaggcaaa ctggggattg gtgaagatgt cgaggagatg
      781 ctgcaatctg acctgcgatt agaactggaa ggcgcgaagg atctgcgtga ggctatcgca
      841 tatgccgaca gcgttcatga ctatgttagc cgcgatatga tgatcgagat ccttgctgat
      901 gaagaaggtc atatcgactg gctcgaaact gaactggatc tcatcggtaa aatcggccta
      961 caaaattacc tgcaatcaca aattaaagtt aaagattaac ggcctatcag caaactatcc
     1021 cagcctataa cgaaacccgc agccgccaga aatggcccaa aaggcagcgg gttttttaat
     1081 gattcttttc ctctcatgag cagaccaata actacggctc cgcaagcgaa tgaggcagca
     1141 aggaaaacca atcgtggtaa gaaggcccat gaatgccagg cgccaagggc ggcgagaaac
     1201 ttcacatcac catagcctaa tccttcttta tgtcgcagta tgcgataccc ccagtaaatg
     1261 acagcgaatg tgccgtaacc aacaattgcc ccccataacg catcggctaa accatcagga
     1321 tgacaaactt gatagaacaa aagacctgac cagagtaacg ggcacgtaaa tctgtcaggt
     1381 aacaagccat gctttgcatc ccagaaaaaa agtagggctg ataaacaagc gtacaaaatt
     1441 agaaagggaa gggtagtgtc cataaaacat cctctgtaat tggagtatca ccatcgaata
     1501 actccggatg ctcagcaaca gagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1561 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1621 ttaatttcta cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1681 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1741 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1801 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1861 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1921 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1981 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     2041 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2101 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2161 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2221 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2281 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2341 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2401 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2461 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2521 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaggac
     2581 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2641 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2701 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2761 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2821 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2881 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2941 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     3001 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3061 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3121 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3181 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3241 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3301 cctggctgcg cgcaacctgc agaaggttga cgtacgcgat gcaactggta tcgacccggt
     3361 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3421 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3481 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgcgaaagac
     3541 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3601 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3661 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3721 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3781 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3841 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3901 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3961 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     4021 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4081 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt aacaccctgc
     4141 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4201 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt gatggtgctt
     4261 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa
     4321 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4381 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4441 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4501 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4561 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4621 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4681 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4741 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4801 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4861 aaataaggta ggaggaagag atggaaacta ttgctaaaca tcgccatgct cgttcttctg
     4921 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4981 atattttgac ctacaccaac aagaaagcgg ctgtacaggt caagaaagtt ctggaatctg
     5041 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5101 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5161 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5221 gagactagca atgggtcaga aagtacatcc taatggtatt cgcttgggta ttgtaaaacc
     5281 atggaactct acttggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5341 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5401 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5461 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5521 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5581 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5641 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5701 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5761 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5821 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5881 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5941 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg
     6001 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6061 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6121 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6181 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6241 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6301 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6361 gcaaaagagc tgcgtgagaa gagcattgaa gagctgaaca ccgagctgct gaacctgctg
     6421 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6481 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6541 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6601 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6661 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6721 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6781 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6841 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6901 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6961 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     7021 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7081 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7141 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7201 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7261 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7321 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7381 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7441 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7501 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7561 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7621 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7681 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7741 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7801 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7861 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7921 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7981 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     8041 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8101 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8161 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8221 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8281 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8341 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8401 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8461 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8521 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8581 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8641 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8701 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8761 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8821 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8881 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8941 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     9001 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9061 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9121 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9181 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9241 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9301 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9361 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9421 gcctaccgtc gtcctgagcc ttacaaaggc aagggtgttc gttacgccga cgaagtcgtg
     9481 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9541 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9601 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9661 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9721 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9781 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9841 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9901 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9961 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
    10021 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10081 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctacaa caccctgtta
    10141 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttccgaaggt accggtatca
    10201 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10261 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10321 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10381 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10441 tgccgaaaca caaggcaacg ctgcttggct tgggtctgcg tcgtattggt cacaccgtag
    10501 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10561 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10621 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10681 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10741 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10801 aagttcgtct atctgacctg gctaaagtag aaggcggtgt tgtagacctg aacacgctga
    10861 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10921 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10981 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    11041 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11101 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11161 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11221 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11281 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11341 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11401 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11461 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11521 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11581 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11641 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11701 gcagtgacgt tctttgttgt gtttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11761 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11821 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11881 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11941 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    12001 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12061 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12121 gtaatgaccc gcctgacctt ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12181 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12241 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12301 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12361 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12421 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12481 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12541 attagccagc caatcttttg tatgtctgta cgtttccatt tgagtatcct gaaaacgggc
    12601 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12661 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12721 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12781 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12841 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12901 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12961 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    13021 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13081 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13141 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13201 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13261 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13321 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13381 tccgccgaaa aaacgtcgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13441 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13501 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13561 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13621 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13681 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13741 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13801 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13861 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13921 ggctgagcag cgtgaaaagc caacttggct ggaagttgat gctggcaaga tggaaggtac
    13981 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    14041 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14101 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14161 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14221 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14281 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14341 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14401 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14461 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14521 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14581 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14641 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14701 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14761 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14821 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14881 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14941 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    15001 tgctggcttc ccgtggactg tctctgggca tgcgcttgga aaactggcca ccggcaagca
    15061 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15121 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15181 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15241 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15301 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15361 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15421 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15481 gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc cgccccggcg ggttttttta
    15541 tacccgtagt atccccactt atctacaata gctgtactct tttt
//
LOCUS       DAJVRP010000090        15398 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVRP010000090 DAJVRP010000000
VERSION     DAJVRP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.090"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..1173)
                     /locus_tag="OQN12_003072"
     CDS             complement(<1..1173)
                     /locus_tag="OQN12_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1655315.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1372..1456)
                     /locus_tag="OQN12_003073"
     tRNA            complement(1372..1456)
                     /locus_tag="OQN12_003073"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Leu,seq:caa)
     gene            1652..2671
                     /gene="ahr"
                     /locus_tag="OQN12_003074"
     CDS             1652..2671
                     /gene="ahr"
                     /locus_tag="OQN12_003074"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1655316.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2800..4302
                     /locus_tag="OQN12_003075"
     CDS             2800..4302
                     /locus_tag="OQN12_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1655317.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="OQN12_003076"
     CDS             complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="OQN12_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1655318.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="OQN12_003077"
     CDS             complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="OQN12_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1655319.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6870..8381
                     /gene="pepA"
                     /locus_tag="OQN12_003078"
     CDS             6870..8381
                     /gene="pepA"
                     /locus_tag="OQN12_003078"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1655320.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8515..8958
                     /gene="holC"
                     /locus_tag="OQN12_003079"
     CDS             8515..8958
                     /gene="holC"
                     /locus_tag="OQN12_003079"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1655321.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8958..11813
                     /gene="valS"
                     /locus_tag="OQN12_003080"
     CDS             8958..11813
                     /gene="valS"
                     /locus_tag="OQN12_003080"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1655322.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11867..13063)
                     /locus_tag="OQN12_003081"
     CDS             complement(11867..13063)
                     /locus_tag="OQN12_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCS1655323.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13256..13759
                     /locus_tag="OQN12_003082"
     CDS             13256..13759
                     /locus_tag="OQN12_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1655324.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="OQN12_003083"
     CDS             complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="OQN12_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1655325.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14383..15396
                     /gene="argF"
                     /locus_tag="OQN12_003084"
     CDS             14383..15396
                     /gene="argF"
                     /locus_tag="OQN12_003084"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS1655326.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3971 a         3930 c         3966 g         3531 t
ORIGIN      
        1 caacctacgc tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg
       61 ctcctggtga ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa
      121 cgaactacaa gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc
      181 cattacgcgc aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc
      241 gcccacaaaa atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag
      301 ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg
      361 cttgactcct tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc
      421 tatttctgac cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag
      481 ttcaaccgcc agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc
      541 aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc
      601 tgcaatctct tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat
      661 cgcggtagta cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc
      721 tttaatgggt acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc
      781 agtaaagaga ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt
      841 ctgattgctg gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg
      901 ttctacctta acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg
      961 agcctcatcc cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc
     1021 cagcatcttc tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg
     1081 gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt
     1141 agccgaacga accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg
     1201 tgggtacaaa cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga
     1261 tgtagattga atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac
     1321 tggattttag gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga
     1381 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1441 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1501 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1561 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1621 ttcccaaaca acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1681 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1741 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1801 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt
     1861 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1921 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     1981 cgagcaaggt gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2041 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2101 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2161 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2221 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2281 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2341 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2401 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2461 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2521 ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga
     2581 actgttcccg atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg
     2641 ctaccgagta gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac
     2701 ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc
     2761 cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt
     2821 gcccgcacgc cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg
     2881 attactggcg caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg
     2941 tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg
     3001 caggcaggaa cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac
     3061 tggcaaccgc atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg
     3121 gtgcgggcga cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac
     3181 gatgtgcaat ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg
     3241 ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc
     3301 ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta
     3361 ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa
     3421 gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa
     3481 ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat
     3541 gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag
     3601 gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata
     3661 aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt
     3721 ccggataatg tgttcgtcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg
     3781 ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt
     3841 gataccgaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat
     3901 gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg
     3961 gggccggtga cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa
     4021 tatgaagatg aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc
     4081 agtaccgagc agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt
     4141 attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa
     4201 gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta
     4261 cgcgggatgt tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgtgc
     4321 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg ttcgaactcg tatttgactt
     4381 tgtcaacaat aataaaggga gctttcgctc ccttgttaga ttacgatttt ctcattaaca
     4441 gccacaggct gattaagaag aagctggcac ttggcaacag tgcgccgatg atcggcggga
     4501 tgccgtaaac caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga
     4561 agctgatacc tgtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa
     4621 acgacagcgc catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca
     4681 tgttgagctg ataacgtccg gcatcctgac cgctcgactt cagatacttc acgtagttgt
     4741 gcaaaccact gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg
     4801 tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg
     4861 tcagatcaga ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg
     4921 cggtagcggc ataacgtacg gattgcagac gacgattctc gttaaaggca taaatactga
     4981 tgccacctaa cacctcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt
     5041 tcgcccataa accttgctgg gtagagagca acgagccgcc gtacatcgcc tgcgcacggt
     5101 agttacgcgc catctgttcg ccctgcggcg cgacccattc gccaatcacc atcgtcagca
     5161 agaccagtgg aatggcggtt ttcatcaccg acagcgctac ctgcatacgg gtaaaaccag
     5221 aagcctgcat caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc
     5281 caagaagagc cgccatcggg aagaagatct gcacatcttt cggcacgctc agcaaggtat
     5341 acattcctgc gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa
     5401 atttgataat gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa
     5461 tagttttacc gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg
     5521 cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc
     5581 caaagccaga taaatcaggt taacggtcca catccacagt gtcgggtcca gcttaccttt
     5641 accgccgttc gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc
     5701 tggcagcatc gacagtacgc gtccctgacg cgggttaacc acgctcaacg gtacaaccat
     5761 aagtgccatc ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc
     5821 acgatcggtg tcagtgttcc acaatgtgcg catgtccatc tggtcggtat cgttcgggtc
     5881 gagcgccacc gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa
     5941 atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga
     6001 gccgtcgcgc agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt
     6061 accttttggt cgaatttgtg cgaggaacac atctttgaaa tcgctgccgt caacgctttc
     6121 gatgaacagc accgagctgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc
     6181 catgccaggg ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg
     6241 tcccgcccac atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc
     6301 cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc attaccgtaa tttcactttc
     6361 ggtatacagt ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag
     6421 ctgcgccatt tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc
     6481 gccgtcaacc gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa
     6541 gatgaagagt atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat
     6601 cactttaaat acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt
     6661 aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga
     6721 ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga
     6781 agtcatcgca acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc
     6841 gttgtcttta agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg
     6901 agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga
     6961 ttgcagaaca gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgccggggcg
     7021 aactggaagg aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg
     7081 agcgaattct ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc
     7141 aggttattca gaaaaccatt aatacgctga atgatactgg ctcgatggaa gcggtctgct
     7201 ttctgactga actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg
     7261 agacagcaaa agagacgctc tacagtttcg atcagttgaa aacgaataag agcgaaccgc
     7321 gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg
     7381 agcgcgcgat ccagcacggt ctggcgattg ccgccgggat aaaagcagcg aaagatctcg
     7441 gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc ctctcaggcg cgccagctgg
     7501 ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc
     7561 tgggaatgca ctcttatctg gcggtcggtc agggttcaca aaacgaatcg ctgatgtcgg
     7621 tgattgagta caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag
     7681 gtttaacctt cgactccggc ggtatctcca tcaagccttc agaaggcatg gatgagatga
     7741 agtacgatat gtgcggcgcg gcggcagttt acggcgtgat gcgtatggtc gcggagctgc
     7801 aactgccgat taacgttatt ggcgtgctgg caggctgtga aaacatgcct ggcgggcgtg
     7861 cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa gtgctgaaca
     7921 ccgatgccga aggccgcctg gtactgtgcg acgtgttaac ttacgttgaa cgttttgagc
     7981 cggaagcagt gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc
     8041 atattactgg tctgatggcg aaccataatc cgctggccca tgaactgatt gccgcgtctg
     8101 aacaatccgg tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacagctgg
     8161 agtccaattt tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag
     8221 gttgcttcct gtcacgcttt acccgtaagt ataactgggc gcacctggat attgcaggaa
     8281 ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac
     8341 agttcctgtt aaaccgcgct gggtttaacg gcgaagagta agtttgcgtt ttaccctcac
     8401 cctaaccctc tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat
     8461 ctttcagggt gaggaatgca gcaatattta aatccaccac aagaagcccc atatatgaaa
     8521 aacgcaacgt tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag
     8581 caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt actcatcgcc
     8641 tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa
     8701 agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcagtgcgcc ggtggagatc
     8761 gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc
     8821 tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct
     8881 ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat
     8941 acggcaacct ggaaataatg gaaaagacat acaacccaca agatatcgaa cagccgcttt
     9001 acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt
     9061 tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc
     9121 agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt
     9181 ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg
     9241 ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct
     9301 gggaatggaa agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact
     9361 ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag
     9421 aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaagcgc ctggtaaact
     9481 gggacccgaa actgcgtacc gctatttctg acctggaagt ggaaaaccgc gaatcgaaag
     9541 gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag
     9601 attatctggt ggttgcgacc actcgcccgg aaaccctgct gggcgatacc ggtgtagctg
     9661 ttaacccgga agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg
     9721 ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct
     9781 gcgtgaagat caccccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc
     9841 tgccgatgat caacatcctg acctttgacg gtgatatctg tgaaagcgcc caggtgttcg
     9901 ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagag ttccagaaac
     9961 tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgatgcactc ggcctgctgg
    10021 aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtggttatcg
    10081 aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg
    10141 aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct
    10201 cctggatgcg cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta
    10261 tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc
    10321 gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca ggacgaagac gttctcgaca
    10381 cctggttctc ttctgcactg tggaccttct ctacccttgg ctggccggaa aataccgacg
    10441 ccctgcgtca gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct
    10501 ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc
    10561 aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggtcagaaga
    10621 tgtccaaatc caagggtaac gttatcgacc cgctggatat ggttgacggt atttcgctgc
    10681 cagaactgct ggaaaaacgc accggcaata tgatgcagcc gcagctggcg gacaaaatcc
    10741 gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc
    10801 gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc
    10861 tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga
    10921 acacagaagg tcaggattgc ggcttcaacg gcggcgaaat gacgctatcg ctggcggacc
    10981 gctggattct ggcggagttc aaccagacta tcaaagcgta ccgcgaagcg ctggacagct
    11041 tccgcttcga tatcgccgca ggcattttgt atgagttcac ctggaaccag ttctgcgact
    11101 ggtatctgga gctgaccaag ccggtaatga acggtggaac agaagccgaa ctgcgcggta
    11161 ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc
    11221 cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca
    11281 ccatcatgct gcagccgttc ccgcagtatg atgcatctca ggttgatgaa gccgcactgg
    11341 ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg taacatccgt gcagaaatga
    11401 acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac
    11461 gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg
    11521 tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa gatcatcgac ggcgcagagc
    11581 tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgc ctggcgaaag
    11641 aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa caagctggca aatgaaggct
    11701 ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg tgagaagctg gaaggttatg
    11761 cagaagcgaa agccaagctg attgaacagc aggctgttat cgccgcactg taatcaacac
    11821 agtgatgaaa acgaaggccg gagcatgctc cggccttttt tatctcttac agaaatggta
    11881 aagaaggcat aactccccga gaaatactgg ctaagaagcc tttatctggt tgttcttctt
    11941 tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt
    12001 caatcatcca tattttcagc agtgggtaag ccagaccacc cgtaatcccg gatatcacca
    12061 ccaacgcaca catgcgataa agcataccgt ggtacgttaa agttgagcga aaacgaatac
    12121 gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac
    12181 tggtgctaac agcaatccca aaataataaa ttaactgcgc gattatcatt ttacgttgca
    12241 tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacccagaa ctatcatact
    12301 catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaagt aataaagcta
    12361 aaaatgcgag gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat
    12421 gtataccgaa actggtattg ctccagagaa aactcattac caggctatat aaagtaccat
    12481 taaaaatacc aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac
    12541 tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa
    12601 tggccattaa aatcggcaag aaaaaggtta cccaccagaa ccctttcaaa gagcaattaa
    12661 aactaaatct tacgccgttg agactggtca ttaaggcctg ataacgcagt ccttttgctg
    12721 ccataaatat aagcaaaacc aacagtaaca aagtcaaaac acagccaaca agcggcatat
    12781 ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa
    12841 aaacattccc accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat
    12901 aaagataacg cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg
    12961 ttaacaacac attcaccata caaataagaa aatatctctc acctgtacca tgaaaaacaa
    13021 aagaatggga cggaacatcc atttcattaa taacttgagc cataaaaatc ccctgaatta
    13081 aaaaatccat tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata
    13141 ctcttatcag taataggctg gcaaaaactt tattgcttta taagacgctt gcgcacgtaa
    13201 gaagagagtg gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa
    13261 taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat
    13321 cgccagcgtc atccgtcagg tttacgcaga gtatggtctt accgccgata aaggctacac
    13381 cgttgccgat ccgaatctcg acgaactgta tcaagtgtac agccagcctg gccatgctta
    13441 ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc gggattgcgc cattaacagg
    13501 tagtgaagcg gacatttgcg aactgcagaa aatgtacttt cttcccgcta ttcgcggtaa
    13561 agggctagcg aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa
    13621 acgctgctat ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt
    13681 gggctttgag catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg
    13741 gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa
    13801 ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt
    13861 cgccgttagg atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg
    13921 ccagcgtcat cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc
    13981 tgaggatgtc gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg
    14041 tcacttcgta accgagttta aacgcttcaa ctgccgcttt ttccagagtt tccagatcgt
    14101 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
    14161 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    14221 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    14281 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataataa tacacataaa
    14341 ttgaatttta attcattgag gcgttagcca caggagggat ctatgtccgg gttttatcat
    14401 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta
    14461 gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag aagccaaact cactggtaaa
    14521 aacatcgcgc tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc
    14581 gcatatgacc agggggctcg cgttacttat cttggcccaa gcggtagtca gattggtcat
    14641 aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat
    14701 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg
    14761 aacggcctga ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag
    14821 gagcatttac ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt
    14881 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
    14941 gtcgcgccac aggcatgctg gccagaagct gcgctggtta cggaatgccg cgccctggca
    15001 caacaaaatg gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct
    15061 gactttatct ataccgatgt atgggtgtcg atgggtgaag caaaagaaaa atgggctgag
    15121 cgcattgcat tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac
    15181 ccggaggtca aattcctcca ttgcctgccc gcgtttcatg acgaccaaac gacgcttggc
    15241 aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc
    15301 gaatctgccg ccagcattgt atttgatcag gcggaaaacc gcatgcatac catcaaagcg
    15361 gtgatggtcg cgacgctcag taaattaaat aattaata
//
LOCUS       DAJVRP010000091        15342 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVRP010000091 DAJVRP010000000
VERSION     DAJVRP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.091"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OQN12_003085"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OQN12_003085"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1655327.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OQN12_003086"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OQN12_003086"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1655328.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OQN12_003087"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OQN12_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCS1655329.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OQN12_003088"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OQN12_003088"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1655330.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OQN12_003089"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OQN12_003089"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1655331.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OQN12_003090"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OQN12_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1655332.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OQN12_003091"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OQN12_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCS1655333.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OQN12_003092"
     CDS             complement(8064..9206)
                     /locus_tag="OQN12_003092"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCS1655334.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OQN12_003093"
     CDS             complement(9215..10228)
                     /locus_tag="OQN12_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCS1655335.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OQN12_003094"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OQN12_003094"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1655336.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OQN12_003095"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OQN12_003095"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1655337.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OQN12_003096"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OQN12_003096"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1655338.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OQN12_003097"
     CDS             complement(12826..13950)
                     /locus_tag="OQN12_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1655339.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OQN12_003098"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OQN12_003098"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1655340.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4378 a         3444 c         3146 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttgg aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctgcc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgacatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgctaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcagca agaattggcg ggtaaattgc tatttaagtc aa
//
LOCUS       DAJVRP010000092        15244 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVRP010000092 DAJVRP010000000
VERSION     DAJVRP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.092"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..63)
                     /locus_tag="OQN12_003099"
     CDS             complement(<1..63)
                     /locus_tag="OQN12_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCS1655341.1"
                     /translation="MTMKKLLIIILPVLLSGCSAF"
     gene            complement(161..1270)
                     /gene="anmK"
                     /locus_tag="OQN12_003100"
     CDS             complement(161..1270)
                     /gene="anmK"
                     /locus_tag="OQN12_003100"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1655342.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1544..2011
                     /gene="slyB"
                     /locus_tag="OQN12_003101"
     CDS             1544..2011
                     /gene="slyB"
                     /locus_tag="OQN12_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1655343.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2058..2492)
                     /gene="slyA"
                     /locus_tag="OQN12_003102"
     CDS             complement(2058..2492)
                     /gene="slyA"
                     /locus_tag="OQN12_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1655344.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2693..2929
                     /locus_tag="OQN12_003103"
     CDS             2693..2929
                     /locus_tag="OQN12_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1655345.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2932..3789
                     /locus_tag="OQN12_003104"
     CDS             2932..3789
                     /locus_tag="OQN12_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1655346.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3789..5786
                     /locus_tag="OQN12_003105"
                     /pseudo
     CDS             3789..5786
                     /locus_tag="OQN12_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5787..6308)
                     /gene="sodC"
                     /locus_tag="OQN12_003106"
     CDS             complement(5787..6308)
                     /gene="sodC"
                     /locus_tag="OQN12_003106"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS1655347.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6389..7285)
                     /locus_tag="OQN12_003107"
     CDS             complement(6389..7285)
                     /locus_tag="OQN12_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1655348.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7334..7573)
                     /locus_tag="OQN12_003108"
     CDS             complement(7334..7573)
                     /locus_tag="OQN12_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1655349.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7676..8275
                     /gene="nemR"
                     /locus_tag="OQN12_003109"
     CDS             7676..8275
                     /gene="nemR"
                     /locus_tag="OQN12_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1655350.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8312..9409
                     /gene="nemA"
                     /locus_tag="OQN12_003110"
     CDS             8312..9409
                     /gene="nemA"
                     /locus_tag="OQN12_003110"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCS1655351.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9490..9897
                     /gene="gloA"
                     /locus_tag="OQN12_003111"
     CDS             9490..9897
                     /gene="gloA"
                     /locus_tag="OQN12_003111"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1655352.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10000..10647
                     /gene="rnt"
                     /locus_tag="OQN12_003112"
     CDS             10000..10647
                     /gene="rnt"
                     /locus_tag="OQN12_003112"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1655353.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10740..15198
                     /locus_tag="OQN12_003113"
                     /pseudo
     CDS             10740..15198
                     /locus_tag="OQN12_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
BASE COUNT         3500 a         4028 c         4013 g         3703 t
ORIGIN      
        1 aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt ttttcatagt
       61 catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct tccaagtgta
      121 acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg gcggggaaaa
      181 tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt cctggtaatc
      241 ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca tcgccactaa
      301 tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc agacgcgcca
      361 tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag ccaccgctca
      421 acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc tgaacatcac
      481 ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag ttaaagtatt
      541 cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc atattttgca
      601 gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg taaggtttac
      661 ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta tcgtagccgc
      721 caaccggctg cccaggaatg agcagtgaca gattggcgat gccgccaata ttgagcacca
      781 ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc agcggtgcgc
      841 cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta attccggtgc
      901 gtgccacaat ttgattgtta tcgccaatct gcaaagtgtg tggtgccacg cccgtcggtt
      961 catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc tgcaggtttt
     1021 gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga gtatcaagct
     1081 gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca gcctgtttca
     1141 gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt tcatcaattg
     1201 tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca ataaagcggc
     1261 ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa acataaactt
     1321 ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca ttatcttcaa
     1381 atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc attatgattt
     1441 gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact ttgaccatga
     1501 gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta aacgcgtatt
     1561 ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca ccctgtcagg
     1621 ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg gcaccatcgt
     1681 taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg gtgcaattgg
     1741 cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg ggcgttctct
     1801 ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac agagtgcaat
     1861 gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata ccatcatggt
     1921 ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac tggccagcaa
     1981 tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca ggttactgac
     2041 cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg ctcaagtttt
     2101 gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa tatttccgcg
     2161 cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt ttccgtcagt
     2221 ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa ccctttttct
     2281 tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc ttttgccagt
     2341 tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac ccaatgggtt
     2401 tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat gcgcaccaac
     2461 cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt agcttgctaa
     2521 gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca aatgtattgc
     2581 tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt tcgcacttaa
     2641 agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact ttgtgaagtt
     2701 tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt ccgtctattt
     2761 tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg tacaccgcct
     2821 gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg atttatcgct
     2881 gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac tatgtcaatt
     2941 aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct tgccggatgg
     3001 tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat acgcgcagaa
     3061 caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat aaaagacaac
     3121 caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt tcagatcgcc
     3181 gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa agccaataac
     3241 gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga actcgatacc
     3301 gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc gacgctgaaa
     3361 cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg atgggtgact
     3421 aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt tgcccttgtc
     3481 gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg ccatatccgc
     3541 gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca gggtcacgtt
     3601 tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag ccttattccg
     3661 gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt ccgctttgcg
     3721 ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag cattgccgta
     3781 ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc agggccacgc
     3841 tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg ctgacggttg
     3901 cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca gtggttagct
     3961 ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc agtttactgg
     4021 gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg ttttttctat
     4081 tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc acgaataacg
     4141 tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt ccgatggtta
     4201 atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc gaggtgatag
     4261 tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc gatgctactg
     4321 cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc agtttactct
     4381 ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt gggcagatac
     4441 tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt cgccagcaaa
     4501 acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc atctccagcc
     4561 tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa attctcgaac
     4621 aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca cgtattatcg
     4681 ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg cagcgactac
     4741 gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg caaagcggtg
     4801 tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc gacaacgccg
     4861 aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc gcatggagta
     4921 ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt agctgcgtac
     4981 tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc acgctggtgt
     5041 tacttttggt caaatttggc ctgatggtcc agattagcga tctgtggcaa tttttactgt
     5101 ttctctttcc actgctggcg acaatgcagc ttcttaaatt gcagatgccc aaatttgccg
     5161 cactgtgggg gcaactgatt gtttttatgg gttcgtttat cgctgtcact aatcccccgg
     5221 tgtatgattt tgctgatttt cttaactata atctggcaaa aatcgttggc gttgcgctgg
     5281 cttggctagc gttcgccatt ctgcgtccag gatcggatgc tcgtaaaagc cgccgccata
     5341 ttcgcgcgct gcgccgggat tttgtcgatc agctaagccg ccatccaaca ctgagtgaaa
     5401 gcgaatttga atcgctcact tatcatcacg tcagtcagtt gagtaacagc caggatgcgc
     5461 tggctcgccg ttggttatta cgctggggtg tagtgctgct gaactgttct catgttgtct
     5521 ggcaattgcg cgactgggaa tcgcgttccg atccgttatc gcgagtacgg gataactgta
     5581 tttcactgtt gcggggagtg atgagtgagc gtggcgttca gcaaaaatca ctggcggcca
     5641 cacttgaaga attacagcgg atttgcgaca gccttgcccg tcatcatcaa cctgccgccc
     5701 gtgagctggc ggcaattgtc tggcggctgt actgctcgct ttcgcaactt gagcaagcac
     5761 caccgcaagg tacgctggcc tcgtaattac ttaattacac cacaggcata gcgttcaccg
     5821 ccaccgccca gcggtttagg ttgatcggac atattatcgc cgccaacgtg gaccatcagc
     5881 gccttgtctt tgatttcatc cagtgatttc agacgaggcg cgatgacggc atcggtagct
     5941 ttgccgtcat tattgacgac cagtgcaggc agatcgccta aatgcccggt accttctggc
     6001 ccttcatgtt taccggtatt ttgtggatca agatgcccgc ctgcggattc cgcggcgctg
     6061 gctttgccat ctttggtggc tggctggcag cttcctttgg catgaatatg gaagccatgt
     6121 tcaccggggg gtaatgcttt cagatcgggt gaaaactcca gacctttatc ggtttcagta
     6181 atggtgacgc taccaattga ctgcccaacc ccttgcgacg tgacgaggtt catctcgact
     6241 ttttcactgg cagcttgtgc gccggtcgca acaaccagcg ccagaatggc cagactaaaa
     6301 cgtttcatag gacctccgtt cattgtgcat tcctgaataa gtgtaaacca gtgacacatt
     6361 tttgaacggc ggctattcct aaaagtgctt acggtacgtc gtaccccagt gcagctttac
     6421 ggatacgaaa ccattgttga cgggtcattt tcagtgtttc tgcttcgaca gctgcccgta
     6481 cgcgctcaat tttacctgag ccgataattg gcagcggctg cgacggtaaa cgtaataccc
     6541 aggcgtaaac cacctgttca atcgagcccg cgtttaactc ctccgccacc acagccagtt
     6601 catcacgcag cggctggaaa taatcatcat taaacagacg accaccacca aggcaggacc
     6661 acgccatcgg acgaacacgc agttgttgta gttggtcgag cgtgccatcc agcagtaacg
     6721 gctgatgcac cggggatatt tccacctgat tagtggcaag ggtaaacggc agacgtgatt
     6781 gcaacagggc aaattgcgca ggcgtaaagt tcgatacgcc aaaatgacgc actttgccgc
     6841 tctgatgcag atgtttgaac gcgtccgcca cttcatcggc atccattaac gggtctggtc
     6901 ggtggattaa cagcagatcc agatgatcgg tcgcgagatt aattagcgac tgttcggcgc
     6961 tcttaatgat gtgattgcgg tcagtgatgt aatgaccaat gacgttttct tcacgcgcgg
     7021 tcgtcgcgat accgcattta ctgacgattt ccatccgttc acgcaggtga ggtgccagtt
     7081 tcagtgcctc gccaaacgcc gcttcgcact gatagccacc ataaatatca gcatggtcca
     7141 cggtggtcac gccgagatcc agatgctctt caataaaact gaccagctgg cgggcggaca
     7201 tattccagtc catcaatcgc cagtagccca tcacaaaacg ggaaaactcc gggccttccg
     7261 gcgcaatagt aatacgctga accataatcg cttcctctta tcagatataa gaggagtata
     7321 cgcaagatta ggttcaaaag agtgatggtt gctccggttc gtctgatgac gctggcttat
     7381 ttgcgcgtaa tttgcgcatt aatcgctgcc gacaaaggcg cagcacctct tgtttttcgc
     7441 catcgctcat tttattccag ttaaaacgct catcccgact acgaaaacag ccgcgacaaa
     7501 acccgcgttc gtcagactgg caaattcccc ggcacgggct ctggacggga aagaactcta
     7561 attgctccgc cacctcgccc tcctcagata agattattac cattattgaa gctgttaatg
     7621 tccaaagtag caactttgct tgcactagac cgactggtct actacactcc aacgcatgaa
     7681 caaacacacc gaacatgata ctcgcgaaca tctcttggcg acgggcgagc aactttgcct
     7741 gcaacgtgga ttcaccggga tggggctaag cgaattacta aaaaccgctg aagtgccgaa
     7801 agggtccttc tatcactact ttcgctctaa agaagcgttt ggcgttgcca tgcttgagcg
     7861 tcattacgcc gcatatcacc agcgactggc tgagttgctg caatccggcg aaggtaacta
     7921 ccgcgaccgc atactggctt attaccagca aacactgaac cagttttgcc aacatggaac
     7981 catcagtggt tgcctgacag taaaactctc tgccgaagtg tgcgatctgt cagaagatat
     8041 gcgcagcgcg atggataaag gtgctcgcgg cgtgatcgcc ctgctctcgc aggctctgga
     8101 aaatggccgt gagaaccatt gtttaacctt ttgtggcgaa ccgctacaac aggcacaagt
     8161 gctttacgca ctatggcttg gcgcgaatct gcaggccaaa atttcgcgca gtttcgagcc
     8221 actggaaaac gcgctggccc atgtaaaaaa cattattgcg acgcctgccg tttagcaggc
     8281 attttttatc accagacgac cgggagcctt tatgtcatct gaaaaactgt attccccact
     8341 gaaagtgggc gcgatcacgg cggcaaaccg tatttttatg gcaccgctga cgcgtctgcg
     8401 cagtattgaa ccgggtgaca ttcctacccc gttgatggcg gaatactatc gccaacgtgc
     8461 cagtgccggt ttgattatta gtgaagccac acaaatttct gcccaggcaa aaggatatgc
     8521 aggtgcgcct ggcatccata gtccggagca aattgccgca tggaaaaaaa tcaccgctgg
     8581 cgttcatgct gaaaatggtc atatggcctt gcagctgtgg cacaccggac gcatttctca
     8641 cgccagcctg caacctggcg gtcaggcacc ggtagcgcct tcagcactta gcgcgggaac
     8701 acgtacttct ctgcgcgatg aaaatggtca ggcgatccgc gttgaaacat ccatgccgcg
     8761 tgcgcttgaa ctggaagaga ttccgggtat cgtcaatgat ttccgtcagg ccattgctaa
     8821 cgcgcgtgaa gccgcttttg atctggtaga gctccactct gctcacggtt atttgctgca
     8881 tcagttcctt tccccttctt caaaccatcg taccgatcag tacggcggca gcgtggaaaa
     8941 tcgcgcacgt ttggtactgg aagtggtcga tgccgggatt gaagaatggg gtgccgatcg
     9001 cattggcatt cgcgtttcgc caatcggtac tttccagaac acagataacg gcccgaatga
     9061 agaagccgat gcactgtatc tgattgaaca actgggtaaa cgcggcattg cttatctgca
     9121 tatgtcagaa ccagattggg cggggggtga accgtatact gatgcgttcc gcgaaaaagt
     9181 acgcgcccgt ttccacggtc cgattatcgg cgcaggtgca tacacagtag aaaaagctga
     9241 aacgctgatc ggcaaagggt taattgatgc ggtggcattt ggtcgtgact ggattgcgaa
     9301 cccggatctg gtcgcccgct tgcagctcaa agctgagctt aacccacagc gtgccgaaag
     9361 tttctacggt ggcggcgcgg aaggctatac cgattacccg acgttgtaat ccaacattgc
     9421 gagcggcgta aagccgccgc tatactaaaa caacattttg aatctgttag ccattttgag
     9481 gataaaaaga tgcgtcttct tcataccatg ctgcgcgttg gcgatttgca acgctccatc
     9541 gatttttata ccaaagtgct gggcatgaaa ctgctgcgta ccagcgaaaa cccggaatac
     9601 aaatactcac tggcgtttgt tggctacggc ccggaaaccg aagaagcggt gattgaactg
     9661 acctacaact ggggcgtgga taaatacgaa ctcggcactg cttatggtca catcgcgctt
     9721 agcgtagata acgccgctga agcgtgcgaa aaaatccgtc aaaacggggg taacgtgacc
     9781 cgtgaagcgg gtccggtaaa aggcggtact acggttatcg cgtttgtgga agatccggac
     9841 ggttacaaaa ttgagttaat cgaagagaaa gacgccggtc gcggtctggg caactaatct
     9901 cctgccgggc gtgaactcat cgcgcccgca tctttactgc atcgacaagt aatatttgtc
     9961 ataatgcgcg ctgcaattta tccgtattaa gagaatcaga tgtccgataa cgctcaactt
    10021 accggtctgt gcgaccgttt tcgtggtttt tatcctgttg tgatcgatgt tgaaacagcc
    10081 ggatttaacg ccaaaaccga tgcgctgctt gagattgccg ccatcaccct gaaaatggat
    10141 gaacaaggct ggctgatgcc ggacaccaca ttacatttcc acgtcgaacc atttgtcggc
    10201 gcaaatttgc aaccagaagc cctcgccttc aacggcattg acccgaacga tcccgatcgc
    10261 ggcgcggtca gcgaatacga ggcgctgcac gaaattttta aagttgtacg taaaggtatt
    10321 aaagcgagcg gctgtaaccg cgccattatg gtggcgcaca acgccaattt tgatcacagc
    10381 tttatgatgg ccgccgcaga acgcgcctcg ctgaaacgta acccgttcca ccctttcgcc
    10441 acttttgaca ctgctgcact ggccgggctg gcactcggac aaaccgtatt gtcaaaggct
    10501 tgccagaccg ctggcatgga cttcgacagc acccaggcgc actccgcgct gtacgacacc
    10561 gaacgcactg ctgtgctgtt ttgtgaaatc gtcaaccgct ggaaacgtct gggaggctgg
    10621 ccgctacctg ccgccgaaga ggtgtaatcg agtcgatgcc tgatgacatg caatgattca
    10681 ggcatctata gtgaggctat tccacgcatc aagcatgata ttcacgggga atagcgttaa
    10741 tggcagataa tccagaccct tcatcgctcc tgccggacgt gttttcaccg gcgacccgcg
    10801 actggtttct tcgcgccttt aaacagccga ccgctgtcca gtcgcaaacc tggcatgtgg
    10861 cggcgcgagg cgaacatgcg ctggtgattg caccgactgg ctccgggaaa acgctggcag
    10921 catttctcta cgccctcgat cggctcttcc gcgaaggcgg cgaagatacc cgcgaggcgc
    10981 ataagcgtaa aacctcacgc atcctctata tttcaccgat aaaagccctg ggcaccgacg
    11041 ttcagcgcaa cttgcagatc ccgttgaagg gtattgccga tgaacggcgg cggcgcggcg
    11101 aaacggtaat tattgatgaa gtccacgcgg tagcgggcag taaacgtggt gcgcatctgg
    11161 cgttaagtct ggagcggctc gatgcgctgc tccacacctc agcacagcga attggccttt
    11221 ctgccactgt gcgctcagcc agcgatgtgg cagcatttct tggtggcgat cgcccggtta
    11281 cggtagtcaa cccgcccgca atgcgccatc cgcagatacg aattgtcgta ccggtcgcca
    11341 atatggatga tgtctcatcg gtcgccagcg gcaccggcga agacagccat gccggccggg
    11401 aaggctccat ctggccatat attgaaacgg gtatccttga tgaagtgttg cgccatcgct
    11461 cgaccattgt ctttactaat tcacgcgggc tggcggaaaa actgacagca cgattaaatg
    11521 agctttacgc cgcacgctta cagcgttccc cgtctatcgc cgttgatgcg gcccatttcg
    11581 agtcgacctc cggcgcaacc tctaaccgtg tacaaagtag cgacgttttt attgcccgct
    11641 cacaccacgg ctccgtctct aaagaacaac gagcaatcac cgaacaggcg ctgaaatcgg
    11701 gtgaattacg ctgcgtggtc gcaacctcca gtcttgaact ggggattgat atgggcgcgg
    11761 tggatctggt gattcaggtg gcaacgccgc tttctgttgc cagtgggtta caacgcattg
    11821 gtcgcgccgg acatcaggtt ggcggtgtat ctaaagggct gtttttcccc cgtacccggc
    11881 gtgatttagt cgattccgca gtcattgtag agtgtatgtt cgcaggcagg ctggaaaacc
    11941 tgacaccacc gcataatcct ctcgacgtcc ttgcgcagca aaccgttgcc gccgcggcga
    12001 tggatgcatt acaggtagac gaatggtact cccgcgtacg ccgtaccgca ccgtggaaag
    12061 atctgccaag acgtgttttt gacgccacgc tggatatgct ttccgggcgc tatccctctg
    12121 gcgatttttc tgcttttcgc cccaaactgg tctggaacag ggagaccggg atattgaccg
    12181 cccgacctgg cgctcaattg ttggcggtta ccagcggcgg caccattccg gatcgtggca
    12241 tgtatagcgt gttattaccc gaaggtgaag aaaaggccgg ttcgcggcgg gtgggtgaac
    12301 tggatgagga gatggtatat gagtcgcggg tgaacgacat tatcactctc ggcgctacct
    12361 catggcggat ccagcaaatc acccgcgatc aggtgattgt gactcctgct ccgggtcgtt
    12421 ctgcccagct ccccttctgg cgtggtgaag gtaacggacg tccggctgaa ttaggcgaga
    12481 tgatcggcga ttttcttcat ttactggcgg atggcgcgtt cttttccggg actattcccc
    12541 cgtggctggc agaagaaaat acgaacgcca atattcaggg gttgattgac gagcaacgca
    12601 acgcgacggg catcgttccg gggagtcgcc atctggtcct cgaacggtgc cgtgatgaaa
    12661 ttggcgactg gcgtattatt ttgcactctc cctatggaag acgggtgcat gaaccctggg
    12721 cgctggcgat tgccgggcga atacatgccc tatggggcgc tgacgcgtct gtggtcgcca
    12781 gtgatgacgg cattgttgcg cgcattcctg acactgatgg taaattgccc gacgccgcga
    12841 tttttttgtt tgaaccagaa aagttgctgc aaattgtccg cgaggcggta ggcagctcgg
    12901 cacttttcgc cgcccgtttt cgcgaatgcg ccgcgcgggc attattaatg ccgggccgca
    12961 cgccgggcca tcgcaccccg ctttggcaac aacggctgcg cgccagtcag ttgctggaaa
    13021 tcgctcaggg atatccggat tttccggtca ttctcgaaac cctacgcgaa tgtctgcaag
    13081 atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg cctgaacggt ggcgaaattc
    13141 aaatatccga tgtaacgacc actacgccct cgcctttcgc cacaagttta ttgttcggct
    13201 atgtcgcgga atttatgtac cagagcgacg ccccgctggc agagcgccgg gcatccgtac
    13261 tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca ggtcgatccg ggggaattac
    13321 tcgacccgca ggtcattcgc caggtggaag aagagttgca acgactggct cctggtagaa
    13381 gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga actggggcca atgaccgttg
    13441 aagacctggc gcaacggcat acaggcagca gtgaagaggt tgcgtcgtat ctggaaaatc
    13501 ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg acaggagcgt cttgcctgta
    13561 tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg gctaccagag tcattgccag
    13621 agatttattt acatagagtc agttacccgc ttcgcgacct ctttctgcgc tatctccggg
    13681 ctcatgctct ggtcacggct gaacaactgg ctcatgagtt tagtctcggt attgccattg
    13741 tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt gatgaatctg caacaaaaca
    13801 tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg ctcgctgcaa gccgccagag
    13861 aagcgacgcg tcccgttgca gccacgacct atgcgcgatt gctgctggaa cgtcagggcg
    13921 tattacccgc caccgatggt agcccggcgc tctttgcctc aacatctcca ggcgtttatg
    13981 agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc cggagtcggt ttacccgcct
    14041 cactctggga aagccagatc ctgcctgccc gcgtacgcga ctattcgcca gaaatgctcg
    14101 atgaattact ggcaaccgga gcggttatct ggtcgggaca aaaaaaagct gggtgaagat
    14161 gacggcctgg tggcactgca tctacagaaa tatgccgcag aatcgttcac tcccgccgaa
    14221 gcggatcagg cgaatcgttc ggcgctgcag caagcgataa tcgctgttct ggctgacgga
    14281 ggagcctggt ttgcacaaca aatcagccaa cggatacgcg ataaaatcgt cgaatcggtt
    14341 gattcctcgg cactgcaaga ggcgttatgg gcgctggtct ggcaaggcgt cattaccagc
    14401 gatatttggg caccgttacg cgccctcacc cgcagcagtt ccaacgcacg cacctcaact
    14461 cgccgcagtc accgggctcg tcgtgggcgt cctgtctatg cacaacccgt ctcgccgcgg
    14521 gtatcttaca acacaccaaa tctggctgga cgctggtcgt tattgcaggt ggagccacta
    14581 aacgataccg aaaggatgct ggcgctggcg gaaaatatgc tcgaccgcta cggcatcatc
    14641 agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat gcaaacactt
    14701 tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga aggtctgggt
    14761 ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc gacacaagcc
    14821 acgcaaacac gccactatac accagtggcg ctctccgcca acgatccggc taatgtgtgg
    14881 ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg taccaacgcg tcgggcgggt
    14941 gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg cggcaaaaaa
    15001 atgctggtct ggcaggaaaa ggaggaatta ctcgccccag agattttcca ggcgctgacc
    15061 accgcactgc gtcgcgaacc aaggctgcgc tttacgctaa cagaagtgaa tgatcttccg
    15121 gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag ttcgccacaa
    15181 gggctggatt ggggatagtt gacatcctcc acgccctgaa agacgtggtt ccctgctacg
    15241 ttca
//
LOCUS       DAJVRP010000093        15100 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVRP010000093 DAJVRP010000000
VERSION     DAJVRP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.093"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..180
                     /gene="yjbD"
                     /locus_tag="OQN12_003114"
     CDS             <1..180
                     /gene="yjbD"
                     /locus_tag="OQN12_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCS1655354.1"
                     /translation="RVLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPE
                     ASFSWSANTSTRGRR"
     gene            complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OQN12_003115"
     CDS             complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OQN12_003115"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1655355.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1533..2480
                     /locus_tag="OQN12_003116"
     CDS             1533..2480
                     /locus_tag="OQN12_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCS1655356.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2550..3356
                     /locus_tag="OQN12_003117"
     CDS             2550..3356
                     /locus_tag="OQN12_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1655357.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3366..3773
                     /locus_tag="OQN12_003118"
                     /pseudo
     CDS             3366..3773
                     /locus_tag="OQN12_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3773..4267
                     /locus_tag="OQN12_003119"
     CDS             3773..4267
                     /locus_tag="OQN12_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCS1655358.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4333..5130
                     /locus_tag="OQN12_003120"
     CDS             4333..5130
                     /locus_tag="OQN12_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1655359.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5141..5929
                     /locus_tag="OQN12_003121"
     CDS             5141..5929
                     /locus_tag="OQN12_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1655360.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5981..7210
                     /gene="sorE"
                     /locus_tag="OQN12_003122"
     CDS             5981..7210
                     /gene="sorE"
                     /locus_tag="OQN12_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCS1655361.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7592..8281
                     /gene="pepE"
                     /locus_tag="OQN12_003123"
     CDS             7592..8281
                     /gene="pepE"
                     /locus_tag="OQN12_003123"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCS1655362.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVLLEAGHRF"
     gene            complement(8372..10003)
                     /locus_tag="OQN12_003124"
     CDS             complement(8372..10003)
                     /locus_tag="OQN12_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1655363.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OQN12_003125"
     CDS             complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OQN12_003125"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1655364.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14106..14930
                     /gene="iclR"
                     /locus_tag="OQN12_003126"
     CDS             14106..14930
                     /gene="iclR"
                     /locus_tag="OQN12_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1655365.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3591 a         3982 c         3937 g         3590 t
ORIGIN      
        1 cgcgtactga ccaactccga aaccaacagc attaagaaag agtacatcga taaattaatg
       61 gtcgagcgtg aggctgaagc gaaaaaagcc cgccaattga agaaaaagca ggcttataaa
      121 ccggatcctg aagcgtcatt tagctggtca gcaaatacgt cgacgcgcgg caggcgctaa
      181 tgaacaatca acgcctgaga ttttcactcg tctgtgtgga cacaatcttc ccctcaccct
      241 aaccctctcc ccgaaggggc gaggggactg tccgagctct ttttggacgt tttcatcagt
      301 ctgaggcgtt aatcagcgtc ccttcttttt acgccccggc gaggtaaaac gcttaccgtt
      361 ggacgccggg cgaccgcctt tttccgccgt tttttccatc ttaacgactg gacgtttgat
      421 gcccgctgtt ttcggtttgg ccttcgcttt aggttttgcc tcggaagagg aattttcgat
      481 gagcttaaag aggtcgatta actcatcgtc ggttaaatag cgccactccc ccagcggaat
      541 gccgcttaag ctgacgttca taatgcgcgt acgttccagc tttttcactt catagccgaa
      601 atgctcgcac atgcgccgga tctgacggtt cagcccctgc actagggtaa tgcggaagac
      661 aaacggcgct tcttttttga ctttgcactt tttcgtcacc gtcccgagga tcggcacccc
      721 cgcccccata ccacgaataa attcatcggt aatcggttta tcgaccgtca ccagatactc
      781 tttctcatga tcgttacccg cacggaggat cttattcacc agatcgccgt gattggtgag
      841 gaaaatcagc ccctgggagt ctttatccag gcggccaatc gggaacacgc gtttgctgtg
      901 gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg gtggtgctga caataccgac
      961 gggcttgttc agggcgataa gtaccaaatc ttcggcttcc cgaggctcaa tcaactgacc
     1021 gtttactttc acaatgtcgc cgggtttcac ctgatcgcca atggtggctc gcttgccatt
     1081 aaggaacaca ttgccttgct cgatatagcg atccgcttcg cggcgtgagc aaattccgct
     1141 ttcgctgatg tatttattta aacggactga tgagtcgggc agcataaatt ctcctgtaaa
     1201 acgcggaata taccgtacct cttagcgggg aaaaacaggg ttatgcggca acgtcgaaat
     1261 taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc cggaagtacg atgcaaattt
     1321 gcctggcaaa ataaggataa tcaacatatt acgaatcgct gcacacctgt gctgatgact
     1381 actacgcaga gaaacaaatg tgcaagagaa agcgctattt ctccccatgc aaagagtgaa
     1441 aaatcgcttt gaaaggccct catgatgggg ttatatcaga tcaagcggtg agaaagcgta
     1501 tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa cagtgacgat atccgtttga
     1561 ttgtgaagat tgcccaactc tattacgaac aggatatgac gcaggcgcaa atcgcgcgcg
     1621 aactgggtat ttaccgcacc accatcagcc gcttgcttaa acgaggccgc gatcagggaa
     1681 tggtcaccat cgccatcaac tatgactaca acgaaaatct ctggctggag cagcaactga
     1741 agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg aaatgatgag gatgaagaga
     1801 ctcaactggc gatgatgggg ttacacggcg cgcaactgct ggatcgcttg ctggagcctg
     1861 gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc actggttgaa aacttgccgc
     1921 aggcggggca atcgcggcag ttaatctgcg taccaattat tggcggaccg tccggtaaac
     1981 tcgaaagccg ctatcatgta aacacattaa cctacagcgc ggcagcgaag ctgaaagggg
     2041 aatcgcatct cgcggatttt ccggctctgc tggataaccc attaattcgt aatgggatca
     2101 tgcagtctca gcactttaaa accatctctg cctactggga taatctggat atcgccctgg
     2161 tgggaattgg ctcaccggcc attcgcgacg gcgctaactg gcatgcgttt tatggtggtg
     2221 aagagagtga cgacctgaat gcccgccagg ttgctggcga tatttgctcg cgcttttttg
     2281 atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa aacgctctct atcgaaatga
     2341 ataaattaaa gcaggcgcga tattccattg gcattgccat gagtgaagaa aaatacagcg
     2401 gaattattgg tgcactgcgt ggaaaatata ttaattgtct ggtaacgaat agcagcacag
     2461 ctgaactatt actgaaataa aaatcttccc gatgtttacc gtaataaaaa ttacggcggg
     2521 tccctattat ctaaaatcag gagtctgtta tgcaaacgtg gttaaatttg caggataaaa
     2581 tcattattgt caccggcggc gcatccggta ttggtctggc gattgtagag gaattattag
     2641 cacaaggcgc gaatgtacag atggtcgata ttcacggtgg cgatggtcaa tatgaaggtc
     2701 ataaaggcta tcagttttgg ccgaccgata tttctagcac aaaagaggta aatcatacgg
     2761 tagcggaaat tatccagcgt tttggtcgca tcgacggtct ggtcaataac gccggggtca
     2821 atttcccgcg tctgctggtc gatgagaaag cgcctgccgg gcagtatgaa ctcaacgaag
     2881 ctgcattcga aaaaatggtc aatatcaacc agaaaggtgt ttttctgatg tcgcaggcgg
     2941 tggcgcgaca gatggtcaaa caacatgatg gcgtgattgt gaatgtttcc tcagaaagtg
     3001 ggctggaagg ctcagaagga caaagctgtt acgccgctac caaagccgcg ctcaatagct
     3061 tcacgcgctc ctggtcgaaa gagctgggta aacacggtat ccgtgtggtc ggtatcgcgc
     3121 ccgggattct ggaaaaaacc gggctacgca cgccggaata tgaagaggcg ctggcgtgga
     3181 cgcgcaatat caccgtcgag cagctgcgtg aaggctatac caaaaacgcc attcctattg
     3241 ggcgcgccgg aagattagca gaagtagctg attttgtttg ttatctgttg tctgaacgcg
     3301 ccagctacat caccggagta accactaaca ttgcgggcgg caaaacgcgc ggctaaggag
     3361 gctttatggt caacgccatt ttttgtgccc acggcaaatt agcttgcgcc atgctggaat
     3421 cggttcagat ggtttacggc aacgccaatg ttgaagccgt tgcatttgtg cccggtgaaa
     3481 acgccggaga cattgcggta aagctggaga agttagtaag cattcacaat caagatgagt
     3541 ggctgattgc cgtggattta cagtgcggca gcccgtagaa tgcggcagca atgcttgcga
     3601 tgcgcaatcc tcgtttgcgg gtaatcagcg gcctttctct gccgctggcg ctcgaactgg
     3661 ttgataacca ggacagcatg aatgttgatg aactgtgtga acacctgacg caaatcgcaa
     3721 agcaaacctg cgttgtctgg aaacaactgg caacgacaga ggaggatttc tgatgaacat
     3781 tacgctcgcc cgcattgatg accgtctgat ccacggccag gtcactaccg tctggtcgaa
     3841 agtggctaac gcccagcgca ttattatctg caatgacgaa gtttataacg atgaagtgcg
     3901 ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag gttaacgtgg tcaatattga
     3961 aaaagccgtc gcggtttatc acaacccgca atatcaggac gaaaccgttt tttatctttt
     4021 cactcgccca caggatgcct tagcgatggt tcgccagggg gtaaaaatag gcaccctgaa
     4081 tattggcggc atggcctggc ggcccggtaa aaaacagtta accaaagcgg tttcattaga
     4141 tgatgatgat attaacgcat ttcatgagtt aaataatctt ggtgtcatac tggatttacg
     4201 cgtcgtagcg tcagatccct caatcaatat tatcgacaaa ataaacgaac agttaatcgc
     4261 aaattaaata atagcgcctg tgtacatatg ctttaacagg catggatgac gacacttaaa
     4321 aggtgacaaa tcatggaaat aagtacccta caaatcattg ctatatttct tttttcctgt
     4381 attgccggaa tgggcagcgt gctggatgaa tttcagactc atcgcccgtt aattgcctgt
     4441 acggtgattg gtttaattct cggtgattta aaaaccggaa taatactcgg cggtacgctg
     4501 gaattgatag ctctctgctg gatgaacgtc ggcgcggcgc aatctccgga ttctgcactc
     4561 gccagcataa tctccgccat tctggttatc gttggtcagc aaagcatcgc caccggaatc
     4621 gccatcgcgt tgcctgtggc tgcggcaggc caggtgctga cggtgtttgc ccgtaccatc
     4681 accgtagtgt tccagcacgc ggcggataaa gcagcagaag aggcgcggtt tcgcactctc
     4741 gatattctgc atgtctccgc gcttggcgtg caggcgctgc gcgtcgccat tccggcactg
     4801 attgtctcac tgttcgtcag cgccgatatg gtcagcaata tgttaaacgc cattcccgaa
     4861 tttgtgaccc gaggactaca gattgctggc ggttttatcg tggtggtcgg ttacgccatg
     4921 gtgctacgta tgatgggcgt gaaatatttg atgcctttct ttttcctcgg tttcctcgca
     4981 ggtggctacc tcgatctcag cctgctggcg ttcggtggcg tcggcgtgat catggccctg
     5041 gtctacatcc agctaaatcc acagtggcgt aaagctgaac ctcaactcca ggccaccgcc
     5101 tccaccgccc ttgaccaact tgacgattaa cggagccatc atggaacaga gaaaaattac
     5161 acgcagcgat ctggtgagca tgtttctgcg ctccaacctg caacaggcgt cctttaactt
     5221 tgaacgtatt cacgggctgg gcttttgcta cgacatgatc cccgccatca agcgacttta
     5281 cccattaaaa gaggatcagg ttgcggcgct caggctacac ctagtgttct tcaataccac
     5341 gccagccgta tgtggcccgg tcatcggcgc ggaaattgat gacggtacca tcaacggcat
     5401 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     5461 gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca ttctcggccc
     5521 gctgctgttc ttctttattt tcaacgcggt gcgtctggcg atgaagtggt atggcctaca
     5581 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg tgctgcaaaa
     5641 actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg tgaccaaatg
     5701 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat gccgccgatg gctccaccgt
     5761 caccatgacc gtgcagaaca ttctcgacca actttgccct ggtttgctgg cgctcggtct
     5821 gacgctacta atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     5881 cctgtttggc ttagggatta tcggcaatgc tctgggcttc ctgtcctgat tcttcgcccc
     5941 ggcacgactg ccgaggccat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg
     6001 tctttatggt aaacgtgatt tacgcctgga aacctttgac cttcctgaaa tgcaggagga
     6061 tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa
     6121 tctgggtgaa aaccataaaa aagtacccga cgatgtgacg accaacccaa tcatcatcgg
     6181 ccacgaattt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc
     6241 gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg attgccccgg
     6301 ctactccttc ccgtgggtag acggcgaagc gacgcatgtg gttattccca acgaggtcat
     6361 ggaacaatat tgcctgctgg catacgacgg cgaaacctat tttgaaggct cactggttga
     6421 accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta
     6481 taaccacacg atggggattc gcccgcaagg gcgcacgctg atcctcggcg gcaccggacc
     6541 aatgggactg ttggcgattg attatgcgct gcatggacca gttaacccgt cgctgctcgt
     6601 cattaccgat accgacaacg acaaattgag ttatgcgcgc aagcactatc catcagaacc
     6661 gcaaacactg attcattatc tcaatgccac cgatgcagca tttgatacgt taatggcgct
     6721 gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac
     6781 tctcgcctct tccttgctgg cgacagatgg ttgcctgaat ttcttcgccg gaccgcagga
     6841 taaacatttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt
     6901 gggcacgtca ggcggcaata ccgacgacat gcgcgcggcg gtcaaactga tcgaagagaa
     6961 aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac
     7021 cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct
     7081 gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcgattc tggcgcgtca
     7141 tcaggggatc tggtccggtg aggcggagca atacctgctc gcccatgcag aggcaatttc
     7201 ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgcccc agcaacttcg
     7261 cgaccggatg tgcggctgat tgctgaagca gcacaatttg cacgagaagg ataaaaaata
     7321 tcagcgcctt aaggggcgct caaattggtg gcaaagtaca cattgtttat gccggatgcg
     7381 gaatgaagcc ttattcggtc tacataattt tgaaaatcca atatattgca gttatctggt
     7441 aggccaggta agcgtagcgc atcaggcaat gctgtgtttg tcatcagtct cagcgcccgg
     7501 tgcaatacgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc
     7561 cttccgtgat ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
     7621 gctgccgggt aaagcctggc tggaacatgc actgccgcta attgctgaac agttgcaggg
     7681 tcgccgctca gcggtgttta tccctttcgc tggcgtaacg cagacctggg atgattacac
     7741 agagaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
     7801 tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac
     7861 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gctccaatta ctgacgtggt
     7921 taaacgtggc gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
     7981 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
     8041 cccgttgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
     8101 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
     8161 gccggaaggt aactggatca cagtgagtaa aggtcacgct acgctgggtg gcccgaacac
     8221 cacttatgtg tttaaggctg gtgaagaagc ggttctgctg gaagctggtc accgttttta
     8281 agtgaattgc cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgcttgac
     8341 gcgtcttatc aggcctacag gattcacaat attaatactc gtcccgtcct tcatcttcat
     8401 ccggctgttc cagcacactg taagccaccg aacaaaacag cgagttcagg cgctgcatat
     8461 ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct ctgtacgttt tgctgatgca
     8521 ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat gcgaaaacga tgtttgctgc
     8581 gacgcaaacg acgagcgctg gtgacatcgc cagagaagaa caccgacatt gccagcttta
     8641 aattactgag caattgctca tagagcgcat ccagttcctt caacccatca agcgaaaatg
     8701 cccgccgtgc tgccagcgat ttatcggcaa tttcgctgcc catgcgctcg acgatatcgg
     8761 aggcctgttc aaggttgagc gacatttcga tgatctccgc ccagcggcgc gactcttcct
     8821 ccgccaactc ttcttttggc atccgcgcca gatacagctt aatggcggtg tagagaacgt
     8881 tgatatcatc tgccagctta cgcagctctt tctcctgccg tggctcgccg tgcatcactt
     8941 tattcagccc ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg
     9001 cgttcgccag agcaagcgtg ggcgtgtcca gcgcgctgac atccagatgt ttaggccgta
     9061 gctgggtatc cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt
     9121 caacaaatgg cagcatgacc aggcaacgta caaggttgta gaagacgtgg aaatagatca
     9181 ccagttccgc ttttggcaat gacagcttcc ccattgtctc tgccagcaaa tggacaaacg
     9241 gcaggataat caggctaccc accagcttaa acagcagact acccagcgcg acacggcggg
     9301 ctgcggcatt ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggttagctc
     9361 caatcaccag acagagcgcc acggggaagg agataatgcc tgcagcggtc agagtcgcgg
     9421 tcagcagtac agcagcaagg ctggagtagc tgataatggc gaacatcgcg ccaatcagcg
     9481 catccagcag aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg
     9541 tgatcggcgt tacggcctgc acaatcaact ccagcgccag caaaatcagc ccaagaccaa
     9601 taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc
     9661 cgataaaaat aagtaacggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta
     9721 gcgccgtccc gacatctgcc cccagcacaa tgaccagagc cggtgcgagg gctaccagat
     9781 cctgagcgac gaacgaggtc accagcatgg tggtggcatt actgctctgt accagtgcgg
     9841 taacgccgat ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag
     9901 tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga
     9961 ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa cacgagttca ggttctcctt
    10021 atcgttatat gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca
    10081 tctttactgt gccttttgac attcgttttt ccggctaaga ttattccagc ctgtttccag
    10141 tataagtgtt tcatattaat aaagagacag ggcactcatt aagcgccctg tttgttgtaa
    10201 ggaaaagtga cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc
    10261 gctcaacatc ggagacgctc atacctttac ggcgggcata gtcttcaacc tgatcgcgct
    10321 gaatttgcgc caccgcgtag tacttgctgt ccggatgact gaaataccaa cccgaaaccg
    10381 atgcaccagg ccacatcgcg aaagattctg tgagtttcat gccagtatgt ttttccactt
    10441 ccagcagctc ccagatggtg gctttttccg tatgttccgg gcaggccgga tagcccggtg
    10501 ccggacggat gccctggtag ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg
    10561 gcgcatagcc ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg
    10621 ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa
    10681 aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat
    10741 ctgctttacc agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaatcccg
    10801 ttttttcggt ctgttgacgc agatggtggc tgacgttgat cacatgggtg cgcgtttcgt
    10861 cacggtagat ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac
    10921 gcggattcag cgttttctcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca
    10981 gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgcggatac ttcccggcca
    11041 gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt acgcagcgtt tcgatgctgg
    11101 cttccacttc ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa
    11161 atgcgaaatc gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgt ggtttcttgc
    11221 gcccgtgctg aatacgcacg gtttcgtact ccttgcaggt acgagcgaca aaatcatcac
    11281 gctgagtatc ggaaagcagc gccgctacca caccaacggt gcgcgaggca ttctgcacat
    11341 acaccgtagg gccgctgtag ttctgctcga ttttcaccgc cgtgtgcgct tttgaggtgg
    11401 tcgcgccgcc aatcagtaac ggaatggcga agccctgacg ctccatctct ttcgccacgt
    11461 taaccatctc gtccagcgac ggcgtgataa gccccgaaag gccaatcaaa tcagcattca
    11521 cttctttggc gatacggaga attttttccg caggcaccat aacgccgaga tcgacaattt
    11581 cgtagttgtt acattgcagc accacaccaa cgatattttt accgatgtcg tggacatcgc
    11641 ctttcacggt ggcgatcacc atcttgccgt tggttttacc ctgctctttg ctggcttcaa
    11701 taaacggttc gagatacgcc actgcctgtt tcatgacgcg cgccgatttg accacctgtg
    11761 gcaggaacat tttgccttcg ccaaacagat cgccgaccac gttcatgccg tccatcaacg
    11821 ggccttcaat cacttcaatc gggcgcgtag cctgctggcg ggcttcttca gtatcctgct
    11881 cgataaactc ggttatgcct ttgaccagcg agtattccag acgtttattc acgtcccacg
    11941 agcgccactc cgcttgctgg gcgttagcag tgtcgtccgc tttactgccg cgatatttct
    12001 cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg attaagaatc acatcttcca
    12061 ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc cagttgcccg gcgttgacga
    12121 tccccatatc catgccattg cgaatagcgt agtagaggaa cactgcgtga atggcttcgc
    12181 gcaccggatc gttgccacgg aatgagaaag aaacgttaga tacgccgccg gaaatcagcg
    12241 cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat aaagtcctgc gcgtagttgt
    12301 tgtgctcttc aataccggtg gcgaccgcga agatgttcgg gtcgaagatg atatcttctg
    12361 gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcgattttcc
    12421 gtgcgcgagt atcggcctgt ccctgttcgt caaaggccat caccaccact gccgcaccgt
    12481 agcgacgcaa caatttcgcg tgatggataa aggcgtcgac gccctctttc atcgagatag
    12541 agttaacaat gcctttgccc tggatacact tcagaccttt ttcaatgaca tcccattttg
    12601 aggagtcgat cataatcggc acgcgagcga tatccggttc accggcaatc agattgagaa
    12661 aacgcaccat cgccgcttcg gcatcgagca tcccttcatc catgttgata tcgataatct
    12721 gcgcgccgtt ttccacctgc tgacgcgcca catccagcgc ctcgctgtat ttctcttctt
    12781 tgatcaggcg cttgaactta gcagagccgg tgacgttggt gcgttcaccc acgttcacaa
    12841 acaggctatc gtcgccaatg ttcagcggct ccaggccaga caaacggcag gctacgggaa
    12901 tttccggcag tttgcgcggc gctaatcctt ctactgcacg actcatcgct gcaatatgtt
    12961 gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa gcccgcttgc gcccattcac
    13021 gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc accaaaggcg ttaggcagcc
    13081 cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg tgatagttcc tgcacgtact
    13141 ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa ggtcagcgct tcggcgtggc
    13201 gcaatgagtt gtaaaatgct tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg
    13261 tgatggtgcc ggagatcata atcggcagct caacgcccag cgcttcaaac tccgttttca
    13321 ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat
    13381 ccgcaccacc ttccaccagc gctttggtgg attcgcgata ggccgccgcc agctggtcaa
    13441 aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga aatagacgcc gtgcggttgg
    13501 ttgggccaag aacaccggca acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcgt
    13561 cagcacaggc gcgcgccagt ttcgccgccg caaagttgat ttccgccgac agggattcca
    13621 tctggtaatc cgccatcgca atggtcgtgg agttgaaggt gttggtttcg atgatatccg
    13681 cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac ttccggttta ctgagtacca
    13741 gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat
    13801 cggcttcgtt cagtcgatag ctctggatca tggtgcccat accgccgtcc aacaccagaa
    13861 tacgttcatt taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca
    13921 cgctcaaccc agatttaata aaaattcaac aaaccatact ggaataaacg catctgtggt
    13981 aaaagcgacc accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc
    14041 aactattgca ttagctaaca ataaaaatga aaataatttc cacgatacag aaaaaggagg
    14101 ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac
    14161 cagcgactgg acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg
    14221 aatccaatgg cagcgtggca ctcacagagc tgtcgcaaca agccgggtta cccaattcca
    14281 cgacccaccg cctgctaacc acgatgcaac agcagggttt cgtgcgtcag gtcggcgaac
    14341 tgggacactg ggcaatcggc gcacatgcct ttatggtcgg cagcagcttt ctccagagcc
    14401 gtaatttgtt agcaattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa
    14461 cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc gattattatc gaccaggtac
    14521 agtgtaccca cctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt
    14581 caggtgcggg taaagccttt ttagcccaac tgagcgaaga acaggtgacg aagctgctgc
    14641 accgcaaagg gttacatgcc tatacccacg caactctggt gtctcctgtg catttaaaag
    14701 aagatctcgc ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg
    14761 ggctacgttg ccttgcagcg tgtattttcg atgagcaccg cgaaccgttt gccgcaattt
    14821 ctatttccgg accgatttca cgtattaccg atgatcgcgt gaccgagttt ggcgcgatgg
    14881 tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg
    14941 cgggcagagg caatattctg cccatcatac ctgagtggca atagaataag ggtgtctgtt
    15001 aatcgcattg acgccaaaat aacttaatgt catacacttc acacactttt attaactcag
    15061 cattattttt aaacatcaaa ccacttaatt attaacaaaa
//
LOCUS       DAJVRP010000094        15031 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVRP010000094 DAJVRP010000000
VERSION     DAJVRP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.094"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..238
                     /locus_tag="OQN12_003127"
     CDS             <1..238
                     /locus_tag="OQN12_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCS1655366.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OQN12_003128"
     CDS             248..886
                     /locus_tag="OQN12_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS1655367.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OQN12_003129"
     CDS             889..2052
                     /locus_tag="OQN12_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1655368.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..3761
                     /locus_tag="OQN12_003130"
     CDS             2136..3761
                     /locus_tag="OQN12_003130"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1655369.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3878..4153)
                     /gene="yjfN"
                     /locus_tag="OQN12_003131"
     CDS             complement(3878..4153)
                     /gene="yjfN"
                     /locus_tag="OQN12_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1655370.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4302..4631)
                     /gene="bsmA"
                     /locus_tag="OQN12_003132"
     CDS             complement(4302..4631)
                     /gene="bsmA"
                     /locus_tag="OQN12_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1655371.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4813..5562
                     /gene="yjfP"
                     /locus_tag="OQN12_003133"
     CDS             4813..5562
                     /gene="yjfP"
                     /locus_tag="OQN12_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1655372.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5559..6314)
                     /gene="ulaR"
                     /locus_tag="OQN12_003134"
     CDS             complement(5559..6314)
                     /gene="ulaR"
                     /locus_tag="OQN12_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1655373.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6422..7486)
                     /gene="ulaG"
                     /locus_tag="OQN12_003135"
     CDS             complement(6422..7486)
                     /gene="ulaG"
                     /locus_tag="OQN12_003135"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1655374.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7841..9238
                     /gene="ulaA"
                     /locus_tag="OQN12_003136"
     CDS             7841..9238
                     /gene="ulaA"
                     /locus_tag="OQN12_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCS1655375.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9254..9559
                     /gene="ulaB"
                     /locus_tag="OQN12_003137"
     CDS             9254..9559
                     /gene="ulaB"
                     /locus_tag="OQN12_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1655376.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9569..10033
                     /gene="ulaC"
                     /locus_tag="OQN12_003138"
     CDS             9569..10033
                     /gene="ulaC"
                     /locus_tag="OQN12_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS1655377.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10047..10697
                     /gene="ulaD"
                     /locus_tag="OQN12_003139"
     CDS             10047..10697
                     /gene="ulaD"
                     /locus_tag="OQN12_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1655378.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10707..11543
                     /gene="ulaE"
                     /locus_tag="OQN12_003140"
     CDS             10707..11543
                     /gene="ulaE"
                     /locus_tag="OQN12_003140"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1655379.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11543..12229
                     /gene="ulaF"
                     /locus_tag="OQN12_003141"
     CDS             11543..12229
                     /gene="ulaF"
                     /locus_tag="OQN12_003141"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1655380.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12358..12633)
                     /locus_tag="OQN12_003142"
     CDS             complement(12358..12633)
                     /locus_tag="OQN12_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1655381.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12960..13355
                     /gene="rpsF"
                     /locus_tag="OQN12_003143"
     CDS             12960..13355
                     /gene="rpsF"
                     /locus_tag="OQN12_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1655382.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13362..13676
                     /gene="priB"
                     /locus_tag="OQN12_003144"
     CDS             13362..13676
                     /gene="priB"
                     /locus_tag="OQN12_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1655383.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13681..13908
                     /gene="rpsR"
                     /locus_tag="OQN12_003145"
     CDS             13681..13908
                     /gene="rpsR"
                     /locus_tag="OQN12_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1655384.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13950..14399
                     /gene="rplI"
                     /locus_tag="OQN12_003146"
     CDS             13950..14399
                     /gene="rplI"
                     /locus_tag="OQN12_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1655385.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14470..>15031)
                     /locus_tag="OQN12_003147"
     CDS             complement(14470..>15031)
                     /locus_tag="OQN12_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1655386.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3512 a         3666 c         4049 g         3804 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaacc gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccaata acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggttccggca
     3421 atattatgtg cctggatgtg ttgcgcgttc tcaataagca agcgggcgta tacgacttat
     3481 tgtcggaagc atttgtggaa gtgaaagggc aggatcgcta ttttgatcgc gcggttcgtc
     3541 gtttacagca gcagctgcgt aagccagctg aagaactggg gcgagagatt actcatcagc
     3601 tattcctgct gggctgcggt gcgcaaatgt tgaaatatgc ttctccgcca atggcgcagg
     3661 cgtggtgtca ggtgatgtta gatacgcgcg gcggcgtacg gttgtcagag cagatccaga
     3721 atgatttatt gctgcgggcg acggggggag tgtgtgtgta agcgtatacg actgatgcga
     3781 cgctggtttc gattaactaa atgaaatatg tgaaaattgt aggccggaca aggcgctcgc
     3841 gccgcatccg gcattgttca tttgctgtag atgacactca tgcatacagt atcgcctgta
     3901 cgcgccagtt atcaggctgc tggtcttcgt acatggtaat aatgcggtac catgacgcac
     3961 cttgttcatc ggcctttaac gccacgatac gttccacgtc ctgcggatcc cctgaaatat
     4021 tgctaactga gatctgacct atttcattca ggcccgtcac gcagtcagca ctggcgaatt
     4081 ctgcagactg tgccgtggcg ctcgcaggat aagtcactaa ttgcagcgag ggtgaggcaa
     4141 gtaattgttt catcgtcagc tccatttttc gtttccccac atgttgcagg gcattccatc
     4201 gcggtaataa acgcccaagc ttccagccgg taatttattg tgcacaggta aacgcatagg
     4261 gacgcaaagc aatgtaaagc tgactatgta agttgtctga tttatttacg gtacaaaatg
     4321 gcttgtgaat accactgtcc tgtcacgatg gtttcgtcca ccatgacgac aacgtaataa
     4381 tcagcttttg cagcgacagc ttttgctctg atttctgcta atgcgtcatc cggagaaccc
     4441 cgaaccatcg tgcttacgct acctattcgc tgtaaccctt gcgtctggtc gcggcgaatc
     4501 tcttgcggat ggtccgttac tggcggtgct ggctgtggcg taccttgcag tgcgctacag
     4561 gcacttaaca tcaacaccaa taataaactg gcaaaccggt aaataacgct attacgtttc
     4621 ctgctaacca tagtgtagtg ccttattaat ttaactttgg gggaatgttc ccgaattgtt
     4681 acctggtacg cgattttcga atgaaaacgt gacgaaagcg taacccacat ctcaacatta
     4741 tgaactaata ccgccagcag aaacttgtgc taaggatttc ttcatgtcat accagacaaa
     4801 aaggagagac agatgattga aatagaatca cgcgagctgg cagatattcc cgttcttcat
     4861 gcttatcctg tcgggcaaaa agataccccg ttaccgtgcg taatttttta tcacggcttt
     4921 acttcatcca gcctggtgta tagctatttt gccgttgcgc tggcgcaggc tggtttgcgg
     4981 gtgatcatgc cggatgcgcc cgatcacggt agccgtttta gtggcgacga agcgcggcgg
     5041 ttaaatcaat tctggcaaat cttgctacaa agtatgcagg aattcactac tttacgtgcg
     5101 gcaatagccg aagaaaactg gctgcttgat gaccgtctgg cagtcggtgg cgcgtcgatg
     5161 ggcgcgatga cggcactggg aattaccgct cgccacccca cggtgaaatg taccgccagc
     5221 atgatgggat cgggctattt tacatcgctc gcccgttcac tgtttccacc gctgatacct
     5281 gaaacggcag cacagcagaa tgaattcaat aaaattgtcg cgccgctggc agagtgggaa
     5341 gcgacaaacc acctggaaca acttggggac agacctctac tgctgtggca tggcctcgac
     5401 gatgacgttg tgcctgccga cgaatcacta cgtttgcagc aggccttaag cgagacggga
     5461 cgggataaac tgctaacctg ttcatggcag ccaggcgtgc gtcaccgtat tacgcctgag
     5521 gcgttagatg ctgccgtgac atttttccgc cagcatcttt aaacacgcag aatgctgacc
     5581 ccttgcgctt ccagttgttg caggatttcc gggttagcat ttttgccggt gatcagcata
     5641 tcgatttgat cggcacggct aaaaagcatt cccgcgcgtt cgccaatctt actgctatca
     5701 accagtacca ccagtttccc taccacgctc agcatcttct gctctgccat tgctgtcagc
     5761 atatcggttt tatacaaccc ttctgcggtc agcccttttc cgctggtaaa catccagtgc
     5821 ccggcataga gactgttttc gctgccctgc gggcttaaag tgatggactg acttttgttg
     5881 tactgtccac ccataatgat cacgctgtca tgttcttgat cgatcaggta atttgccagc
     5941 ggtagataat tagtgatgat ttgcactggc ttgccacaca tttcccgccc aagcagaaac
     6001 gcggtggagc cgcagttgat gactacgctt tcgcccggat taaccagctg cgaggccgct
     6061 ttagcaatac gtactttttc atcgtgattc tgcgcctgat gcagattcat cggcgtccag
     6121 cgcgggcgct gttgggtaat agcttctgcg ccattgcgca cctttttcag tttgccgctt
     6181 tcgccaagtt tattgatatc gcgtcgcgca gtggcaggcg aaattcccag acgctcaacg
     6241 actttctcaa cggtcacaaa gcccaattgt gcgagcattt ccaggaggat ttgatgtctt
     6301 tgtgcttcag tcatgagcta ttccgataaa atttaatttt tttagatgat atttgaaata
     6361 gccaggaaat actacgccgg atagcgcgaa cttctccacg ctatccggta taaacgacag
     6421 attacaggaa tgacttgaac ggcagatccg gttcaatagt gaagcaatca tcgaaaccgc
     6481 gcggatagtg gtactcgaag ttgtctttat ccagcggcca ggtaaatttg ccgccaacct
     6541 gccagataaa cggcttgaag ccatacttca gacgatcttt tttgatctcc cacagcacgc
     6601 ggatctcttg cggatcggcc tggaagtttg accagatgtc gtggtggaac gggataacca
     6661 ctttcgcatt cagggcttca cccatacgca gcatatcggc gctggtcatt ttgtcggtaa
     6721 taccgcgcgg gttctcacca tacgatccta acgccacatc gatctgatgt tcgttaccgt
     6781 gcttcgcata atagttagag tagtgggaat cgccactgtg atatagggaa ccgccagggg
     6841 ttttgaacag gtagtttact gcgcgatcgt ccatgccatc tggcagtacg ccagccgctt
     6901 tttgatcggc aggcagggtg atcagtgcag tacggtcgaa agcgtcaagc gcatgaattt
     6961 caatgtcttt cacttttaca acatcgcccg gtttgaccac gatgcaacgc tctttcggta
     7021 cgccccagcc aatccacaaa tcaacgcagg ttttcggtcc aatgaatggt acgtcatcag
     7081 cacagttctg catcacggca gcagcgacgt taacgtcgat atgatcgttg tgatcgtgag
     7141 tcgccagtac cgcgtcgatc tggcgaatcg caaacggatc aagaacaaac ggggtggtgc
     7201 gtaggtttgg ctgcagtttt ttcacgccag ccatgcgctg catctggtga ccctgtttca
     7261 ttaacgggtt accgtgactt tgtttgccag tgccgcacca gaaatcaacg caaacgttgg
     7321 tgccaccttc tgatttcaac cagatcccgg tgcagcctag ccaccacatc gcaaatgtgc
     7381 caggggcgac ctgctcttgt tcaatttctt cattcagcca gctaccccac tccgggaaag
     7441 tgctcaggat ccaggattct cgggtgatac ttttcacttt actcatcgcc atttaccttc
     7501 atgatagttc aattcgaatc aacatgtgat tggttttgat taatcctgac actattcctt
     7561 caggaaggca atgaccattt tttgactttt tgccagggaa gttgttgttg attttgagtg
     7621 tggaaagatt taatggaatg tgtagttaaa ttaactaaat gaatttaaat aaataattgt
     7681 ttcgttgtgt gaatcacact ctatccgtgt ggaattattt gcgggtcgcg ccacatttaa
     7741 tcataaataa tcttgttgtg attacttttg aaaattagag tgagtacaca acattccggg
     7801 tgtgcggaat acccggttac ctcttcttca ggagatcgtt atggagatcc tctacaacat
     7861 ctttaccgtg ttttttaacc aggtcatgac caatgccccg ttgttgctgg gtattgtgac
     7921 ctgtctgggc tacatcctac tgcgcaaaag tgtcagcgtt attattaaag gcacgattaa
     7981 aaccataatt ggtttcatgt tgttgcaggc agggtccggc atcctcacca gcaccttcaa
     8041 accggtggtg gcgaaaatgt ccgaagtcta cggcattaac ggcgcaattt ccgatactta
     8101 cgcgtcgatg atggcaacca tcgaccgcat gggcgatgcc tatagctggg taggttacgc
     8161 ggtgttgtta gcgctggcgc tgaacatctg ttacgtgctg ctgcgtcgca ttaccggtat
     8221 tcgtaccatc atgctgactg gtcatatcat gttccagcag gcggggctga ttgccgttac
     8281 gttgtttatt ttcggctact ccatgtggac aaccattatc tgcacggcga ttctggtttc
     8341 gctctactgg ggcattacct ccaacatgat gtacaagccg actcaggaag tgacggacgg
     8401 ctgcggtttc tccatcggtc accagcaaca gtttgcatca ttaattgcct ataaagttgc
     8461 gccgttcctc ggcaaaaaag aggaaagcgt tgaagacctc aaactgccag gctggctgaa
     8521 tattttccac gacaacatcg tctccacggc gattgtcatg accatcttct ttggtgccat
     8581 tctgctctcc ttcggtatcg acaccgtgca ggcgatggca ggcaaagtgc actggacggt
     8641 ctatatcctg caaaccggtt tttccttcgc ggtggcgatc ttcatcatca ctcagggtgt
     8701 gcgcatgttt gtggcggaac tctctgaagc gtttaacggt atctcccagc gcctgatccc
     8761 tggtgcggtt ctggcgattg actgtgcggc tatttatagc ttcgctccga acgccgtggt
     8821 ctggggcttt atgtggggca ccatcggtca gctgattgcg gttggcatcc tggtcgcctg
     8881 cggttcctcg atcctgatta tcccaggctt tatcccgatg ttcttctcca acgccaccat
     8941 cggcgtgttc gctaaccact tcggcggctg gcgtgcggcg ctgaagattt gtctggtgat
     9001 ggggatgatc gaaatctttg gttgcgtctg ggtggtgaaa cttaccggta tgagtgcctg
     9061 gatggggatg gcggactggt cgattctggc accgccgatg atgcagggct tcttctccat
     9121 cggtatcgcc tttatggccg tcatcattgt aattgcactg gcttatatgt tcttcgctgg
     9181 ccgcgcactg cgcgcagaag aagatgcaga aaaacaattg gcagaacagt ctgcttaata
     9241 aggagttttg attatgaccg tacgtattct ggctgtgtgt ggcaacggac aaggcagttc
     9301 catgatcatg aagatgaaag tggaccagtt tttaacccaa tcaaacattg accacacggt
     9361 aaatagctgc gcggttggcg aatacaaaag cgagttgagc ggcgcggata tcatcatcgc
     9421 ttctacgcac attgcgggcg aaatcaccgt gaccggcaac aaatacgtgg ttggcgtgcg
     9481 caacatgctc tctcctgccg actttggccc gaaactgctg gaagtgatca aagcgcattt
     9541 cccgcaggat gtgaagtaag gacgcgccat gaaattacat gattcgctgg cggagaataa
     9601 atccatccgc ctgcaggctg aagctgagac atggcaggac gcagtaaaaa tcggcgttga
     9661 cctgctggtg gcggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt
     9721 tgaacagcac ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc
     9781 ggaagagggc gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt
     9841 caaccacgaa gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa
     9901 cacgcaccag gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt
     9961 cgaccgttta cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa
    10021 cgcggcagct taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca
    10081 accagactat ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta
    10141 tcgaagtggg taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag
    10201 cgctctaccc gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc
    10261 tttctcgtat gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata
    10321 tcaacaccgc caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg
    10381 aactgaccgg ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc
    10441 aggtggttta tcaccgcagc cgtgacgcgc aggccgctgg cgtggcgtgg ggtgaagccg
    10501 atatcactgc gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc accggtggcc
    10561 tggcgctgga agatctgccg ctgttcaaag gcattccaat tcatgtgttt atcgccggtc
    10621 gcagcatccg tgatgccgct tctccggttg aagccgcgcg tcagttcaaa cgttccatcg
    10681 ccgaactgtg gggctaagga gcggatatgt tgtccaaaca aatcccgctt ggcatctatg
    10741 aaaaagcgct ccccgccggg gagtgctggc tggaacgcct gcgactggca aaaacgttgg
    10801 gcttcgattt tgtcgagatg tcggtagatg aaactaacga gcgcctgtcg cgcctcgact
    10861 ggagccgcga acagcgtctg gcgctggtga atgctattgt tgaaaccggc gtgcgtgtgc
    10921 cgtccatgtg cctttctgcc catcgtcgtt tcccgttggg cagcgaagat gacgcggtgc
    10981 gggcgcaggg gctggagatt atgcgtaaag ccatccagtt cgcccaggac gttggcattc
    11041 gcgtgatcca gctggcgggc tatgacgttt actatcagga agccaataac gaaacgcgtc
    11101 gtcgtttccg tgacggcctg aaagagagcg ttgagatggc gagccgcgcg caggtgacgc
    11161 tggcgatgga gatcatggat tatccgttga tgaactccat cagcaaggcg ctgggttacg
    11221 cgcactatct caacaacccg tggttccagc tctacccgga tatcggcaac ctgtcggcgt
    11281 gggacaacga tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc gcggtgcatg
    11341 tgaaagacac caaaccgggc gtcttcaaaa acgtgccatt tggcgaaggt gtggtggatt
    11401 tcgaacgttg cttcgaaacg ctcaaacaga gtggctattg cgggccgtac ctgattgaga
    11461 tgtggagcga aacggcggaa gacccggcgg cagaagtggt gaaagcgcgc atggccaaag
    11521 caggcatggt ggaggcggca taatgcaaaa gctaaaacag caggtgtttg aagccaacat
    11581 ggatctgccg cgctatgtgc tggtgacctt tacttggggc aacgtcagcg ctatcgaccg
    11641 cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca tgaaagcaga
    11701 cgatatggtg gtggtcgata tgagcggcaa ggtggtggaa ggggagtatc gcccatcttc
    11761 cgacactgcg acgcatctcg aactctaccg tcgttacccg tcgcttggcg gcattgtcca
    11821 tacccactcc actcatgcca ccgcatgggc gcaggcgggg ctggcgatcc cggcattagg
    11881 caccacgcac gctgactact tctttggtga tatcccgtgt acgcgtggat taagcgaaga
    11941 agaggtgcag ggtgagtatg aactgaacac tggcaaagtg attatcgaaa cgctgggcaa
    12001 cgccgagccg ctgcatacgc cggaaattgt ggtgtatcag cacggaccgt tcgcctgggg
    12061 gaaagatgcc cacgacgcgg tgcataacgc ggtagtgatg gaagaagtgg cgaaaatggc
    12121 gtggattgcg cgcagtatta atccacaact caatcatatc gacagctacc tgatgaataa
    12181 acacttcatg cgtaagcacg gtcctaacgc ttattacggg cagaagtaaa acacgcgctg
    12241 cggaaatttc cttcctcggg agataactgg tctaattccg cagccgtttt tcaaaaaaag
    12301 ccccctgcga agggggcaaa gcaaactatg gcaatgtttc gttggttata cctggtgcta
    12361 gcgataaata tccgcgctgg cgtgcatatt gccgttactc cccggttccc gcatcagaat
    12421 tacgtggtag tacgttgcgc cttgcgcatc tgtttcttca tttaatgcct gacgtgcttc
    12481 gctttcagtg gcgaaattat gattgatata aatcacgcct aagctttgca catcgtccat
    12541 atttctggcc tggtggttat taatttcaat ggctgcccat gtatttgcac ttagcaaaag
    12601 cacagccaga agggctaaaa ctcgactgaa catagatacc tcctcgacgg ctgactttgt
    12661 gtgctctcct ctgtgatgat cttctgattt aattttaatc aatgataaag aagttgatgg
    12721 tgaccatttc tgatgcagtt gttcaaaaaa acactgtgac ggaatgtgat gaacttcaaa
    12781 tcagcggttt agaggttatt tgcgaaaggg gagatttatt tctgctccgc ccttgagttt
    12841 agcggggtat gccagtacta taacgcgtca tttttcagcc gacctttaac acgttccttg
    12901 cctccccggg attcggctga cccagacagg aggctgaata atccgtaagg agcaattcga
    12961 tgcgtcatta cgaaatcgtt tttatggtcc atcctgacca gagcgaacag gttccgggca
    13021 tgatcgagcg ctacactgct gccatcactg gtgcagaagg caagatccac cgtctggaag
    13081 actggggccg ccgtcagctg gcttacccga tcaacaaact gcacaaagca cactacgttc
    13141 tgatgaacgt tgaagctccg caggaagtga tcgatgagct ggaaactacc ttccgcttca
    13201 acgatgccgt tatccgcagc atggttatgc gtaccaagca cgctgttacc gaagcatctc
    13261 cgatggttaa agcgaaagac gagcgccgtg agcgtcgcga tgatttcgca aacgaaaccg
    13321 ctgatgatgc tgaagctggg gattctgaag agtaatttct gatgaccaac cgtctggtgt
    13381 tgtccggcac cgtgtgcagg actccccttc gaaaggtcag tccatcagga attcctcact
    13441 gccagttcgt gcttgagcat cgttctgtgc aggaggaagc cggctttcac cggcaggcat
    13501 ggtgtcaaat gcccgttatt gttagcggac acgaaaacca ggccattact cacagtataa
    13561 cggtcggcag tcgcataacc gttcaggggt tcatttcatg ccacaaggca aagaacggac
    13621 tgagcaaaat ggttttgcat gccgagcaga ttgaattgat agattctgga gactagccat
    13681 atggcacgtt atttccgtcg tcgcaagttc tgccgtttca ccgcggaagg cgttcaagag
    13741 atcgactata aagatatcgc tacgctgaaa aactacatca ccgaaagcgg taagattgtc
    13801 ccaagccgta tcaccggtac ccgtgcaaaa taccagcgtc agctggctcg cgctatcaaa
    13861 cgcgctcgct acctgtccct gctgccgtac actgatcgcc atcagtaatc ggtcacggtc
    13921 cattaatacg actttgagag gataaggtaa tgcaagttat tctgcttgat aaagtagcaa
    13981 acctgggtag cctgggtgat caggtaaacg ttaaagcggg ctatgctcgt aacttcctgg
    14041 taccgcaggg taaagctgtt ccagctacca agaaaaacat tgaattcttc gaagcacgtc
    14101 gcgctgaact ggaagctaaa ctggctgaag ttctggcagc tgctaatgct cgcgctgaga
    14161 aaatcaatgc actggaaact gttaccatcg cgtctaaagc tggcgacgaa ggtaaactgt
    14221 tcggttccat cggtactcgc gacatcgctg acgctgtaac tgcagctggc gttgaagtgg
    14281 ctaagagcga agttcgtctg ccgaacggcg ttctgcgtac cactggcgaa cacgaagtga
    14341 gcttccaggt tcacagcgaa gtattcgcga aagtgatcgt aaacgtagta gctgaataat
    14401 tcgttattca acgagacgta aaaacgccga ccattggtcg gcgttttgct ttctattttt
    14461 cgtcaggcat taatttcgca agtagatcca ttcaccattc tcttgtgtcc ataagtcagg
    14521 atggtttgct ggcgttagat gcatacgatc actccttatg tctcggtcct gcggcactcg
    14581 ctcgaaacct aaacccgagt agattgcggc tgaatttgat aaggaacgta gaccaatttt
    14641 caaatcatgg ggtaaatcag aatacgcgtt tagcaatgca gcaatgatga atccgagggc
    14701 attagaaacg cctgtaacag tgtgaagttg gtcgatataa agatcgattg ttggtagttc
    14761 agatgaccag caattaggca tttgggcagg aagggttaca acgtatccta tcgtttggcc
    14821 ggtatagtct ggagtactat aaccaggtcc caaaacaacc tcgtgtattg tgtatttact
    14881 gttgggttcg aaggattcga catcttgcaa taaatatttc agttctgcgg ttaacctcgc
    14941 acccgcgtaa gctttattgt atttctgggc gtgaatatta tctaatgtag cttcatggtt
    15001 ccatccaaaa gaattatcac cattcattgt t
//
LOCUS       DAJVRP010000095        15007 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVRP010000095 DAJVRP010000000
VERSION     DAJVRP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.095"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..176)
                     /locus_tag="OQN12_003148"
     CDS             complement(<1..176)
                     /locus_tag="OQN12_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655387.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHG"
     gene            complement(592..1533)
                     /gene="fabY"
                     /locus_tag="OQN12_003149"
                     /gene_synonym="yiiD"
     CDS             complement(592..1533)
                     /gene="fabY"
                     /locus_tag="OQN12_003149"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1655388.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="OQN12_003150"
     CDS             complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="OQN12_003150"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1655389.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2012..2884)
                     /locus_tag="OQN12_003151"
     CDS             complement(2012..2884)
                     /locus_tag="OQN12_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCS1655390.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="OQN12_003152"
     CDS             complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="OQN12_003152"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1655391.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3576..4361)
                     /locus_tag="OQN12_003153"
     CDS             complement(3576..4361)
                     /locus_tag="OQN12_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1655392.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="OQN12_003154"
     CDS             complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="OQN12_003154"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCS1655393.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5459..6355
                     /gene="yihU"
                     /locus_tag="OQN12_003155"
     CDS             5459..6355
                     /gene="yihU"
                     /locus_tag="OQN12_003155"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS1655394.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6379..7257
                     /gene="yihT"
                     /locus_tag="OQN12_003156"
     CDS             6379..7257
                     /gene="yihT"
                     /locus_tag="OQN12_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCS1655395.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7274..8515
                     /gene="yihS"
                     /locus_tag="OQN12_003157"
     CDS             7274..8515
                     /gene="yihS"
                     /locus_tag="OQN12_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCS1655396.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8653..9555
                     /locus_tag="OQN12_003158"
                     /pseudo
     CDS             8653..9555
                     /locus_tag="OQN12_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9754..11790
                     /gene="yihQ"
                     /locus_tag="OQN12_003159"
     CDS             9754..11790
                     /gene="yihQ"
                     /locus_tag="OQN12_003159"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCS1655397.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11836..13210
                     /locus_tag="OQN12_003160"
                     /pseudo
     CDS             11836..13210
                     /locus_tag="OQN12_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13253..14656
                     /locus_tag="OQN12_003161"
     CDS             13253..14656
                     /locus_tag="OQN12_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1655398.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14724..>15007
                     /gene="ompL"
                     /locus_tag="OQN12_003162"
     CDS             14724..>15007
                     /gene="ompL"
                     /locus_tag="OQN12_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCS1655399.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGL"
BASE COUNT         3689 a         3869 c         3729 g         3720 t
ORIGIN      
        1 aaaccatgct tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg
       61 caaagtgaaa attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt
      121 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      181 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      241 gtaaatgaat gatactgaca atgtatgcca gcccattatt tttgcataat atttgtgagc
      301 tatagatttt aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa
      361 aaatcgctca aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc
      421 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      481 caacgaagaa taaaaattta taaagttctt attcaatata accggaatag tcagggactg
      541 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      601 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      661 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
      721 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc
      781 atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt
      841 tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc
      901 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg
      961 catggtggtg ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact
     1021 aagcgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1081 aatatcatcc agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1141 tggcgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1201 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1261 catcgccatc agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg
     1321 aatggacgct tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg
     1381 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1441 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1501 ttcttctgtt tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga
     1561 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1621 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1681 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     1741 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     1801 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     1861 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     1921 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     1981 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2041 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2101 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2161 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2221 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2281 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2341 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2401 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2461 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2521 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2581 tcatcttgtt ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2641 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     2701 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
     2761 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     2821 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     2881 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     2941 aatgcctagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3001 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3061 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3121 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3181 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3241 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3301 ctctgcgaac gcttcatcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3361 atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag
     3421 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3481 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3541 gcaaggtatg aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg
     3601 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
     3661 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
     3721 gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta
     3781 gcttcgttta catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt
     3841 accagataat cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg
     3901 ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa
     3961 agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac
     4021 gcccgggcaa catgctcaac agtcgtacca atggtgataa atattgttga accatcagga
     4081 atatagtctg ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc
     4141 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
     4201 atcaggcctt gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc
     4261 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
     4321 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
     4381 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
     4441 agcccggctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
     4501 actgactccg ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca
     4561 tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca
     4621 ccattttcca gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg
     4681 agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga
     4741 cccggttctg aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc
     4801 gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt
     4861 ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt
     4921 tcctccaacc actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc
     4981 tcgcctttgg tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
     5041 gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg
     5101 tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct
     5161 gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc
     5221 tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca
     5281 acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt
     5341 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag
     5401 acaagcaaag catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagggttat
     5461 ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt
     5521 gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt
     5581 agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat
     5641 cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt
     5701 ttgcgaaggc ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca
     5761 aaccgataaa ttgattgccg atatgcaagc caaaggcttc agcatgatgg atgttccggt
     5821 aggccgtact tctgcaaatg ccattaccgg tactctgtta ctgctggctg gcggcaccgc
     5881 tgaacaagtt gaacgtgcca caccgatcct gatggcgatg ggcagtgagt tgatcaacgc
     5941 tggcggtccg ggcatgggga tccgcgttaa gctcatcaac aactacatga gcatcgcgct
     6001 caatgcgctt tcggcagaag ccgccgtttt gtgcgaagcc ctgaatcttc ccttcgatgt
     6061 tgccgtcaaa gtgatgagcg gtaccgccgc cggtaaaggt cacttcacca cttcctggcc
     6121 aaacaaagtc ctcagcggag atctttctcc cgccttcatg atcgatcttg cccataagga
     6181 tcttggcatc gcccttgatg tcgccaacca gctgcatgtg ccaatgccgc tgggggccgc
     6241 ctcacgggag gtttatagcc aggcgcgcgc agcgggtcac ggtcgccagg actggtccgc
     6301 cattctggaa caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa tgtaacgact
     6361 ggataaagga ataaatgaat gaataagtac accatcaacg acattacgcg cgcatcgggc
     6421 ggttttgcca tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct
     6481 ggggcacccg ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa cgctgcaaag
     6541 gccctctcgc cttatgcctc ggcgattctg gtagatcaac aattctgcta ccgccaggtg
     6601 gttgagcaaa acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt
     6661 cctggcaacg gtattccggt cgatagcgtg gttattgacc gcaaaatcaa tccgctacag
     6721 atcaaacaag acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat
     6781 gcgcagcaac gtctggatat ggtgaaagag ttcaacgaac tgtgccactc acacggtctg
     6841 gtaagcatca ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa
     6901 caagcgatca tcgatgccgc caaagagctg ggcgacagtg gcgctgacct ctacaaagtt
     6961 gaaatgccgc tttatggcaa aggtccgcaa caagagcttc tctgtgcttc acaacgtctg
     7021 aatgaccata tcaatatgcc atgggtgatc ctctcttcca gtgtcgacga aaaactgttc
     7081 ccgcgtgccg tacgcgtggc aatgacggcg ggcgcatcgg gattcctggc aggccgtgca
     7141 gtctgggcat cggtcgtcgg tttaccagac aacgagctga tgctccgtga cgtttgcgca
     7201 ccgaaattac aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgctaagat
     7261 gaggataaaa ggaatgaaat ggtttaacac cctaagccac aaccgttggc tggaacagga
     7321 aaccgaccgc atctttgatt ttggtaaaaa ttccgtagtg ccgactggtt ttggctggtt
     7381 aggcaataaa gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat
     7441 gttgcacgtt tattccgttg ctgcggcgat gggtcgacct ggcgcttacg cgttggttga
     7501 tcacggtatt aaagccatga acggcgcact gcgtgataaa aaatatggcg gctggtatgc
     7561 ctgcgtgaat gacgagggcg tggtggatgc ctccaaacag ggctatcaac acttctttgc
     7621 tctgctgggt gccgccagcg ccgtcacaac gggtcacccg gaagcgcgca agctgctcga
     7681 ttacaccatt gagattatcg agaaatattt ctggagcgaa gaagagcaga tgtgcctgga
     7741 gtcctgggac gaagccttca gcaaaaccga agagtaccgc ggcggcaatg ccaatatgca
     7801 cgcggtggaa gctttcttga ttgtttatga cgtcactcac gacaaaaaat ggctggatcg
     7861 cgcgattcgc gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt
     7921 taacgaacac ttcgataccc agtggaatcc gctgccggat tacaacaaag ataacccggc
     7981 gcatcgcttc cgcgcgttcg gaggtacacc aggccactgg atcgaatggg gccgtttaat
     8041 gctgcacatc catgctgccc tggaagcccg ttgcgaacaa ccaccagcat ggctgctaga
     8101 agatgccaaa ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggagcgga
     8161 cgggattgtt tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtacgttg
     8221 gcctatcgtc gaagcaatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca
     8281 gtatgaaacc tggtatcaaa catggtggga gtactgcatt aagtacctga tggactatga
     8341 aaatggttcc tggtggcagg agctggatgc ggacaataag gtcatcacca aagtctggga
     8401 cggcaaacag gatatttatc acctgctgca ttgcctggtg atcccgcgta tcccgttagc
     8461 gcccggcatg gctccagcgg ttgcagcggg tctgctggat attaatgcga aataactgat
     8521 ctgaaaatag ggtgcgggtt ttcccctcac cctaaccctc tccccaaagg ggcgagggga
     8581 ccgtccggtg cagtttttgg gtttgacatc agcctcacgg gaggctgtat gtcgttaatt
     8641 aaggtgcctt gtatgcaaat caccaatatg cattgcagtg gacagaccgt ttcacttgcc
     8701 gcgggggatt atcacgcgac cattgtgacc gtcggtgctg gtctggctga attaaccttt
     8761 cagggttgtc atttagtcat cccgcacaaa cccgaagaga tgccgctggc gcatctgggt
     8821 aaagtgctta ttccgtggcc gaaccggatc gccaacggct gttaccgtta tcaggggcag
     8881 gaatatcagt tacccattaa tgaacatagt tcaaaagccg ctattcacgg gctgcttgcc
     8941 tggcgagact ggcaaattag tgaattaacc accaccagcg ttaccctgac agcttttctg
     9001 ccgcccagtt acggctatcc gtttatgctg gcttcacaag ttgtttattc gctcaacgca
     9061 cacacgggtc tttccgttga gatcgcaagc cagaatatcg gcactgtcgc agcgccttat
     9121 ggcgtgggta ttcatcctta tttaacctgc aacttaacat ctgttgatga atatctgttc
     9181 cagcttccgg caaactaggt ttatgccgtc gatgaacacg taaatccgac aacgcttcac
     9241 cacgttgatg agctggattt aaatttcacg caggcgaaaa aaatagccgc gacaaaaatc
     9301 gaccacacct ttaaaaccgc taatgatttg tgggaaatga ccattaccca tccacagcag
     9361 gcgctttcgg taagcctttg ttccgatcaa ccctgggtgc aggtttatag cggtgaaaaa
     9421 ttacagcgtc agggactggc agttgaacct atgagttgcc cgcctaacgc ctttaattca
     9481 ggtatcgact tattattact ggaaccagga aaaccgcacc gcctgttttt taatatttac
     9541 ggacagagaa aataacttgt attgattagc agtcaaaatc attgaagaac atttcactcg
     9601 ctaaaggcaa cattatgtat cttcaattta ttaagttaaa aacagctcag cttctgtaaa
     9661 gtttttatat atcagtttta tgagaatcat tttacataag cctgtaataa caggaaggca
     9721 acaatctcgt tctcaactct atggagatta gttatggata cgccacgtcc acagttatta
     9781 gattttcaat ttcatcagaa taacgacagt tttaccctac attttcaaca acgtcttatt
     9841 ttaacccata acaaagataa tccttgttta tggattggct caggtatagc ggatatcgat
     9901 atgttccgcg gtaatttcag cattaaagat aaactacagg agaaaattgc gcttaccgac
     9961 gccatcgtca gccagtcacc ggatggttgg ttaattcatt tcagccgtgg ttctgacatt
    10021 agcgccacgc tgaatatctc tgccgacgat cagggccgtt tattgctgga actacaaaac
    10081 gacaacctta accacaaccg tatctggctg cgccttgccg ctcaaccaga ggaccatatc
    10141 tacggctgcg gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt cccgctatgg
    10201 accagtgaac aaggcgttgg tcgcaacaaa caaacctatg tcacctggca ggccgactgc
    10261 aaagaaaatg cgggcggcga ctattactgg actttcttcc cacagcctac gtttgtcagc
    10321 acgcagaagt attactgcca tgttgataac agttgctaca tgaacttcga ctttagcgcc
    10381 ccggaatacc atgaactggc gctgtgggaa gacaaagcta cgctacgttt tgaatgtgct
    10441 gacacataca tctccctgct ggaaaaatta actgccctgc tgggacgcca gccagaactg
    10501 cccgactgga tttatgacgg ggtaacgctc ggcattcagg gcgggacgga agtgtgccag
    10561 aagaaactgg acaccatgcg taacgcgggc gtgaaggtta acggcatctg ggcgcaggac
    10621 tggtctggga tccgtataac ctcttttggc aagcgcgtga tgtggaactg gaagtggaac
    10681 agcgaaaact acccgcaact ggattcacgc attaagcagt ggtatcagga gggcgtgcag
    10741 ttcctggcct atatcaaccc gtatgttgcc agcgataaag atctctgcga agaagcggca
    10801 caacacggct atctagcaaa agatgcctct ggcggtgact atctggtgga gtttggcgag
    10861 ttttacggcg gcgttgtcga tctcactaat ccagaagcct acgcctggtt caaggaagtg
    10921 atcaaaaaga acatgattga actcggctgc ggcggctgga tggctgactt cggcgagtat
    10981 ctgcccaccg acacgtacct gcataacggc gtcagtgccg aaattatgca taacgcctgg
    11041 cctgcgctat gggcgaagtg taactacgaa gcccttgaag aaacgggcaa gctcggcgag
    11101 atcctcttct ttatgcgcgc cggttctacc ggtagccaga aatactccac catgatgtgg
    11161 gcgggcgacc agaacgtgga ctggagtctc gacgatggcc tggcgtccgt agtcccggcg
    11221 gcgctgtcgc tggcaatgac cggacatggt ctgcatcaca gcgacattgg cggttacacc
    11281 accctgtttg agatgaagcg cagcaaagag ctgctgctgc gctggtgcga tttcagcgcc
    11341 ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg gcagtttgac
    11401 ggcgatgcgg aaactatcgc ccattttgcc cgtatgacta ccgttttcac caccctgaaa
    11461 ccttatctga aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt catgcgcccg
    11521 ctgttcctgc attacgaaga cgatgcacat acctattccc tgaaatatca gtacctgtta
    11581 ggccgcgaca ttctggtcgc tccggtacac gaagaaggtc gcagcgactg gacgctctat
    11641 ctgccggagg ataactgggt acatgcctgg acgggtgaag cgttccgggg cggggaagtt
    11701 accgttaatg cgcccatcgg caagccgccg gtcttttatc gcgccgatag cgaatgggcg
    11761 gcactgttcg cgtcgttaaa cagcatctaa tcacatccgc ccgtggggaa actcatgggc
    11821 aaagggagaa catcgatgag tcacatcaca acggaagatc cagcaacttt acgcctgccc
    11881 tttaaagaga aactctctta cggtattggc gacctggcct ctaacatcct gctggatatc
    11941 ggtacgcttt atcttttgaa gttttatacc gacgttctgg ggctgccagg cacctatggc
    12001 ggcattatct ttttgatttc aaaattcttt actgcgttta ccgatatggg taccggcatt
    12061 atgttggatt cccgacgcaa gatcggtcca aaaggtaagt tccgtccttt tattctgtat
    12121 gcgtcattcc cggtcacctt actggcgatc gccaactttg tcggcacacc gtttgatgtc
    12181 accggtaaaa cggtgatggc cactattctg tttatgcttt acggactgtt tttcagcatg
    12241 atgaactgct cctacggcgc aatggtcccg gcaatcacca aaaaccccaa cgaacgcgcc
    12301 tcactggcgg catggcgtca gggcggcgct acgctgggcc tgctgctgtg cacggtggga
    12361 ttcgtgccag ttatgaatct tatcgaaggt aatcagaaac ttggctatat cttcgccgcc
    12421 acgctgtttt cactgttcgg cctgctgttt atgtggatct gctactcagg cgtgaaagag
    12481 cgttatgtcg aaacccaacc aaccaatccg gcgcaaaagc ctggcctgtt gcaatctttc
    12541 cgcgcaattg ccggtaaccg cccactgttc attctgtgta ttgccaacct ctgcacctta
    12601 ggggcgttta acgtcaagct cgccattcag gtctattaca cccagtacgt acttaacgat
    12661 cccatcctgt tgtcgtatat gggatttttc agcatgggct gtattttcat cggcgtgttc
    12721 ctgatgcctg gcgcagtcag acgttttggt aagaagaagg tctatatcgg cggcctgctg
    12781 atttgggtgc tgggcgatct gctcaactat ttcttcggcg gcggctcggt cagctttgtg
    12841 gcgttctcct gcctggcgtt cttcggctca gcgcctgaac tgggcgctgg tttccgacac
    12901 cgtcgagtac ggcgagtggc gcaccggcgt gcgttcggaa ggcacggtct acaccggctt
    12961 caccttcttt cgcaaagtgt ctcaggcgct ggctggtttc ttccccggct ggatgctgac
    13021 gcaaattggc tatgtgccga acgtcgcaca ggctgaccac accattgaag ggttacgcca
    13081 gttgatcttc atctacccaa gcgcactggc ggtagtcacc attgtggcga tgggttgctt
    13141 ctacagcctg aacgagaaga tgtatgtccg cattgtggaa gagatagaag cccgtaaacg
    13201 cacggcgtaa taataataaa cgacgccctg cggggcgtta taaggagtga ttatgtctga
    13261 ccataatcca ctgacattaa aactgaatct gcgggaaaaa atcgcctatg gtatgggcga
    13321 cgtcggttcg aatttaatgc tctgcatcgg tactctgtat ctcctcaaac tttataccga
    13381 tgaattaggg atgcctgctt actatggcgg gattatcttt ctggtcgcga agttttttac
    13441 cgcgtttacc gatatgctca ccggattttt actcgactcg cggaaaaata ttgggcctaa
    13501 aggcaaattt cgtcccttta ttttatatgc agcagttccg gcggcgttaa ttgcaacgct
    13561 ccagtttatc gccaccacct tttgtttgcc ggttaaaacg acgattgcca ccacgctgtt
    13621 tatgatgttt ggactttcat acagtctgat gaactgctcg tatggtgcga tgatcccggc
    13681 aattaccaaa aacccgaatg agcgcgcgca gctcgcggct taccgccagg gtggtgcaac
    13741 catagggcta ttgatttgta ccgtggcgtt tattccgttg cagtcgcttt tttctgactc
    13801 aaccgtcggt tatgcctgtg cggcacttat gttctccatt ggcggcttta tttttatgat
    13861 gctgtgctac agaggcgtca aagagcatta tgtggacaca gcgccaaccg gacataaagc
    13921 cagtattctc aaatcttttt gcgcgatatt tcgtaatcca ccattgctgg ttttatgcat
    13981 tgctaacctg tgtactctgg cggcatttaa tatcaaactg gcgattcagg tctattacac
    14041 ccagtatgtg ctgaatgata ttaatttatt gtcgtggatg ggattcttca gtatgggatg
    14101 catcctcgtc ggcgtattac tggtgccagt aactgtaaaa tgttttggta aaaaacaggt
    14161 ttatttagct ggcatggtgc tatgggcggt gggtgatata ctgaattatt tctggggaag
    14221 taactctttc actttcgtca tgttctcttg tgtcgccttt tttggcacgg cctttgtaaa
    14281 cagcctgaac tgggcactgg taccagatac cgtagattac ggtgaatgga aaaccggtat
    14341 tcgtgccgaa ggttctgttt ataccggtta taccttcttt cgtaaaattt ctgccgcact
    14401 tgctggcttc ttgccaggca ttatgctgac gcaaattggt tatgttccca acatcgcgca
    14461 aagcgatgcg acattacagg gtttgcgtca gctcattttt atctggcctt gtgcactggc
    14521 aattattgct gcattgacga tgggattctt ttacacactc aatgaaaaac ggtttgcatt
    14581 gattattgag gaaatcaacc aacgcaaaaa taaagagatg gcgacagaaa aaaaaacggc
    14641 ttccgtaacg ttataaatgt caatagccgc tatttccatc ctggtgggtg gcggcctccc
    14701 tacgtttaaa aaatggactt attatgaaaa agattaatgc aataatttta ttatcttctc
    14761 tgacctctgc ctcggtattt gctggcgcat acgtcgaaaa cagggaggca tataatcttg
    14821 cctccgatca gggcgaggtt atgctacgtg tgggttataa cttcgatatg ggcgcgggta
    14881 ttatgttaac caatacctac acctttcagc gagaagatga actaaaacat ggctataatg
    14941 aaattgaagg ttggtatcct ttattcaaac caaccgataa attaaccatc cagcccggtg
    15001 gcttaat
//
LOCUS       DAJVRP010000096        14875 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVRP010000096 DAJVRP010000000
VERSION     DAJVRP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.096"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(121..546)
                     /gene="arsC"
                     /locus_tag="OQN12_003163"
     CDS             complement(121..546)
                     /gene="arsC"
                     /locus_tag="OQN12_003163"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS1655400.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(559..1848)
                     /gene="arsB"
                     /locus_tag="OQN12_003164"
     CDS             complement(559..1848)
                     /gene="arsB"
                     /locus_tag="OQN12_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS1655401.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1902..2255)
                     /gene="arsR"
                     /locus_tag="OQN12_003165"
     CDS             complement(1902..2255)
                     /gene="arsR"
                     /locus_tag="OQN12_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1655402.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2998..3081
                     /locus_tag="OQN12_003166"
     CDS             2998..3081
                     /locus_tag="OQN12_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1655403.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3135..4487)
                     /gene="gorA"
                     /locus_tag="OQN12_003167"
     CDS             complement(3135..4487)
                     /gene="gorA"
                     /locus_tag="OQN12_003167"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1655404.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4559..5401)
                     /gene="rlmJ"
                     /locus_tag="OQN12_003168"
     CDS             complement(4559..5401)
                     /gene="rlmJ"
                     /locus_tag="OQN12_003168"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1655405.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5604..7646
                     /gene="prlC"
                     /locus_tag="OQN12_003169"
     CDS             5604..7646
                     /gene="prlC"
                     /locus_tag="OQN12_003169"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1655406.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7654..8406
                     /gene="rsmJ"
                     /locus_tag="OQN12_003170"
     CDS             7654..8406
                     /gene="rsmJ"
                     /locus_tag="OQN12_003170"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1655407.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8455..9924)
                     /gene="dtpB"
                     /locus_tag="OQN12_003171"
                     /pseudo
     CDS             complement(8455..9924)
                     /gene="dtpB"
                     /locus_tag="OQN12_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10242..10676)
                     /gene="uspA"
                     /locus_tag="OQN12_003172"
     CDS             complement(10242..10676)
                     /gene="uspA"
                     /locus_tag="OQN12_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1655408.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11070..11405
                     /gene="uspB"
                     /locus_tag="OQN12_003173"
     CDS             11070..11405
                     /gene="uspB"
                     /locus_tag="OQN12_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1655409.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11476..12975)
                     /gene="pitA"
                     /locus_tag="OQN12_003174"
     CDS             complement(11476..12975)
                     /gene="pitA"
                     /locus_tag="OQN12_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1655410.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13207..14406
                     /locus_tag="OQN12_003175"
     CDS             13207..14406
                     /locus_tag="OQN12_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS1655411.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3706 a         3785 c         3915 g         3469 t
ORIGIN      
        1 agtaagttgg cagcattacc tgaatttgca cggttttgta ggtcggataa gacgcgttag
       61 cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga tatcgccgct
      121 ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct tggagaatgc
      181 gcctttttgc gcatctggca gaatttccag caccacttct gaagggcggc acaggcgagt
      241 tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa gcataaagtc
      301 gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat acggttcgac
      361 gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt tgaccagttc
      421 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
      481 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      541 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      601 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
      661 agtcatattc ttctgcgaaa gtacgtgcag ccagagcagc gttgccaggc taccaatcgg
      721 ggtaattttc ggccccaaat cgcagccaat cacgttggca taaaccatcg cttctttgat
      781 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
      841 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
      901 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
      961 caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc agggggcacc
     1021 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
     1081 tgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccaaga cgaaaaatcc
     1141 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
     1201 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
     1261 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
     1321 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
     1381 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     1441 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     1501 aagcgccgcg ccatcattgg caaacagggc cgcaacggcg gcaccgagca ggacaatcca
     1561 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa acgtgcagcg ctgcccattc
     1621 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcaataa acgccgccgt
     1681 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatgaacca cgcccgtaac
     1741 caacgccagt accgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     1801 gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
     1861 ataaaacaca tgtgcaaatc catatgtgtt tagctaaatt tttaactgca aatgttctta
     1921 ctgtctccgg aacagttttg tctggccagg ttgcggacaa ttgcctgaat cttttcctgt
     1981 tcacatcgcc aggcttcatc aataattttc gccgcccatg ctggaatatg cggcgataag
     2041 cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     2101 gccagatggc gggatatctt gggctgcgac tgatcgaggg cagtgcagag atcgcagacg
     2161 cataactctc ccagttcgct aagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2221 attttgaaca attggatggg taacagaaat gacatattgc gctcctgatt gttgcaggta
     2281 gtgtctctct tcgaagcgga taagtcaaaa acatatatga cttaacgaat gtgtaagtgc
     2341 agaggaaggt aataggtgtg aattttgagt tggctattca tttgaaaggc ggtttgaatc
     2401 aggacgtaaa aaaaccgact ttgcgtcggt tttttactct tcagcctgag ttggtggctc
     2461 tggctggagt gagaatgccc acatctaacg gttcaatact attagccagt ggctaacatt
     2521 gcaagcgtta gcgatttctt cagctggaga agggacagtt tgctatacat ggtggtgaaa
     2581 gaggatttat atactttcat cgggtcgctg ccgtaccaag ccttgtccga atccggacgt
     2641 aaaaaaaccg acttcgcgtc ggttttttta ctcttcagcc cagagttggt ggctctggct
     2701 ggagtgagaa tgccatcatc taacgcatca atgctaatac catactgaaa ctattgcaag
     2761 gttatgctgg ttatttagcc taattacact gtatgattat ccagttagct ctgaggcatt
     2821 ttcactctgg caatgcgcat aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc
     2881 gttaactgat gctctggccg gagtgagatt gcccacatct aacaatgagg catgcgccgt
     2941 gacaggcagt ggatgagtaa gcggatgcat tctcactcca tcgcatggag aaaacgggtg
     3001 attgataaag caatcatcgt tcttggggcg ttaattgcgc tgctggaact gatccgcttt
     3061 ctgcttcagc ttctgaactg atagcggaaa cgtaattaag ggctaagagc acactactct
     3121 tagcccttta acatttaacg catggtcacg aactcttctg ccgccgttgg gtgaatggcg
     3181 acggtattgt cgaagtcttt tttggttgcg cccatcttca gcgccaccgc gaagccctgc
     3241 aacatttcgt ccataccaaa gccaatgccg tgaataccga caatcttctc ttctggccca
     3301 acgcacacca gcttcatgcg gcacggctgg cggtgagtgg tgacggcggt atacatcgcg
     3361 gtgaaagagg atttatacac tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt
     3421 tccgttaaac caacagtacc aatcggcgga tggctgaaga ccacggtcgg aatgttgctg
     3481 taatccagat gctcatccgg cttgttatta aacaggcgtt cagagagacg gcgacccgct
     3541 gcaactgcca ccggtgtcag ttccactgca cccgtgttat cgcccaccgc gtaaatacct
     3601 tcaatattgg tgttttgata tttatcgacg acgatatagc ctttttcgtt ggttttaacg
     3661 ccagcggctt cgaggttgat gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg
     3721 caatccaccg tttcactgcg accatcttcc agctccagcg tcaggctacc atcggcattt
     3781 ttcactaccg ctttcgggat ggcgttggta tgcaactgtg ggccttcggc gttcatcact
     3841 tcgaccagcg tttcagagat catcgggtcg aagctgcgca gcggcgcatg tttacgcaca
     3901 aacagatgcg ttttcgcgcc gaggccgtta atcacgccag ccagctcaac ggcgatgtaa
     3961 cccgcgccaa caaccgccac gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa
     4021 tcaataccgt attccacgcc tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc
     4081 agaatatgat cggccgtgat ggtttcgccg ttaacttcca gcgttttggc atcaacgaag
     4141 cgggcaaagc ctttgattac atcaacgtta tttttaccga gcacgttttc ataggaagta
     4201 tgaatacggt cgatataggc ggtacggctg gcgatcaacg tttcccagtt gaacttgttg
     4261 atggtggtat cgaaaccata atcagggccg tacatatgga tcgcttcacg gatttgcgcc
     4321 gcgtgccaca tcactttttt cggcacgcag ccgacgttca cgcaggtgcc gcccagctct
     4381 ttggcttcaa tcagtgcgca tttctggccg tacatagccg cgcggttgat ggaggcgata
     4441 ccgccgctgc cgccgccgat ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct
     4501 tatcgttgat taccgcgatt gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt
     4561 actccggcac gatccagctt acggtggcgt gcccggtacc tgccggaacc agtttgctgt
     4621 gcagccacga cagcacgtta ttcatctgct gttccagctt ccacggcggg ttaatcacaa
     4681 tcatgccgga agcggtcatg ccacggcgat cgctgtctgg cagcaccgcc agttcaattt
     4741 gcagaatttt gcgaatgccg gtcgcttcca ggtcgtggat catgcgctta atttgctggc
     4801 gcagcaccac cggataccac aatgcgtaag tgccagtggc gaaacgtttg taaccttctg
     4861 ctatcccgct gaccaccgcc tgatagtcgg ttttcatttc atacggcggg tcgataagga
     4921 ttaaaccacg gcgagaaacc ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt
     4981 tttcgacgcg tgcacggctg tctttctgaa atccagaacg cagcaacggg taatcgctcg
     5041 ggtgcagttc ggtcagttgc aggctgtctt gttcgcgcag tagctggcga gcaatcaacg
     5101 gcgaaccggg gtagtaacgc aactggccgc tacggttgaa gtgttttacc acattgatgt
     5161 acgcctccag ttctgcgggc aaatcgtcct gctgccagat acgggcgata ccttcgagat
     5221 attcgccggt acgctcggca tgttcgctac ctaactgata acgcccggcc cctgcgtggg
     5281 tgtcgagata gagaaacggt ttatctttct ctttcagcga ctcgatgatc aggctctgaa
     5341 cggtatgttt aaggacgtcg gcgtggttgc cagcgtgaaa gctgtggcga taactgagca
     5401 tgggtaaagg tgttccggta agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat
     5461 caggacagta taccgaaaag aggccgccgc cgcgaaagcg taacgtttct cattgaaatt
     5521 cactacactt aaccccatgc tacacacatt atgtaaagcg cctgttgagc gcttccttaa
     5581 cctctttaac caggactgcg ctaatgacga atccgttact gactcccttt gaattgcctc
     5641 cgttttctaa aattctcccg gaacatgtcg ttccagccgt gactaaggca ttgaacgact
     5701 gccgcgaaaa tgtggagcgc gtagtagcgc aaggggcacc gtacacctgg gaaaatctct
     5761 gccagccgtt ggcggaagtg gacgatgttt tggggcgtat cttctccccg gtcagccacc
     5821 tgaactcggt gaaaaatagc ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc
     5881 tgtcggaata cagcacctgg gtagggcaac atgaagggct gtataaagcg taccgcgacc
     5941 tgcgcgatgg cgatcattac gccacgctga acacggcgca gaagaaagcg gttgataacg
     6001 ccctgcgtga ttttgaactc tctggcatag gtctgccgaa agagaaacag cagcgttacg
     6061 gcgaaattgc tacccgtctt tctgaactgg gcaaccagta cagcaacaac gtcctcgatg
     6121 cgacgatggg ctggaccaaa ctcgttaccg acgaagcggg gctggcgggg atgccagaaa
     6181 gcgcgctggc tgcggcaaaa gcccaggccg aagcgaaaga gctggaaggc tacctgctga
     6241 cgctggatat cccaagctac ctgccggtaa tgacctactg cgacaaccag gctctgcgcg
     6301 aagagatgta tcgcgcttac agcacccgcg cctccgatca aggcccgaat gccggtaagt
     6361 gggataacag caaggtgatg gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc
     6421 tgggctttga aaactacgcc tttaaatccc ttgccactaa aatggcagaa aacccgcagc
     6481 aggtgctgga tttcttaacc gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc
     6541 tggagcaact gcgtgccttc gctaaagccg aatttggtgt cgatgagttg cagccgtggg
     6601 atatcgctta ctacagcgaa aaacagaaac agcacctcta cagcatcagc gacgagcagc
     6661 tgcgtccgta cttcccggaa aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta
     6721 tttacggcat caccgctaaa gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt
     6781 tcttcgaact gtatgacgaa aataacgaac tgcgcggcag cttctacctc gatctgtatg
     6841 cccgtgaaaa caagcgcggc ggggcgtgga tggatggctg cgtaggccag atgcgtaaag
     6901 ctgatggttc tctgcaaaaa ccggtcgcgt atttgacttg taacttcaac cgcccggtaa
     6961 atggtaaacc ggcgctgttc actcacgacg aagtgatcac cctgttccac gagttcggtc
     7021 acggcctgca ccatatgctg acccgcatcg aaaccgctgg tgtttccggt atcagcggtg
     7081 tgccgtggga tgcggtcgaa ctgccgagtc agtttatgga aaactggtgc tgggagccgg
     7141 aggcgctggc gtttatctct ggtcactatg aaaccggcga accgctgccg aaagagttgc
     7201 tggataaaat gctggcggcg aagaactacc aggcggcgct gtttattctg cgtcagctgg
     7261 agttcggcct gtttgatttc cgccttcatg ccgagttccg cccggatcag ggggcaaaaa
     7321 tcctcgaaac tctggcagaa atcaagaaac tggttgccgt ggtgccatct ccgtcctggg
     7381 gccgtttccc gcacgctttc agccatattt tcgccggtgg ttatgcggca ggttactaca
     7441 gctacctgtg ggctgacgta ctggcggcag atgctttctc gcgctttgag gaagagggca
     7501 ttttcaaccg tgaaaccggg cagtcgttcc tcgacaacat tctgagccgt ggcggttcag
     7561 aagagccgat ggatctgttc aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc
     7621 tggagcatta cggcattaag ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca
     7681 ggcgccggag acggcgcctt atctgttctg gcggcccgct gggggctgga gcacgatgaa
     7741 gacaacctga tggcgctggt gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag
     7801 ccaaaacttg gcggcatctt tgttgatttt gttggcggag cgatggcgca ccgacgcaaa
     7861 ttcggcggtg gtcgcggtga ggcggtggcg aaagcggtgg gcattaaagg cgattatttg
     7921 ccggatgtgg tggacgccac cgcagggctg gggcgcgatg cctttgtact ggcttcagtc
     7981 ggctgccgcg tgcggatgct ggagcgtaat ccagtggttg ccgcgctact cgacgacggc
     8041 ctggcgcgtg gttatgcgga tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta
     8101 attcacgcct ccagcctgac ggcgctaacc gatattaccc cacgcccgca ggtggtctat
     8161 ctcgatccga tgttcccgca taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc
     8221 tttcagtcgc tggtggggcc ggatcttgat gccgatggat tactggagcc tgcacgcttg
     8281 ctggcgacca aacgcgtggt ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc
     8341 gccacgccaa acgcggttgt gactaaaggg catcgctttg atatctatgc aggtacgcca
     8401 gtgtaaaagt aaagcagggt agcgataacg ctaccctgct ttctgcaaga ataattaatg
     8461 gctttccggc gtcgcaatca tgcgtttcag ccacggcacc atcaacagca tcactactgc
     8521 aacgcccagc gtgaccagac caatcttacc aaacacgttg gtatagacgg gcaacgtctc
     8581 aagcggatcg gtaatgttgt ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa
     8641 gaacgcggca gcctgcgtca ggaaccacat cccgagaata aagcccatca aatgctgcgg
     8701 caccagggca gcaatcatcg ccaggccaag ggcgctaata aacaattcac ccaggctcta
     8761 gaataaatac accagcacga taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca
     8821 cattcccgca gcagcagccg tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa
     8881 tttcatcggc atcgagaggt ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag
     8941 tattgggctg gcgagtacca cccagaacgg gttaagcgcc tggaagctga ccgggttgat
     9001 ggaaaaaccg agaatttcat gatgcacgtt gttgatggca aagaagttca gcgatgttgg
     9061 catctgggcg tagagaatgt aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa
     9121 cattttattg cgcccggttt tatccagctt gaatgcctga cgaaagaaga tgatagtgac
     9181 gacgatggag agaacaatca gcaccagatt ggcgacttct acgttgtgca tcagccatgc
     9241 acatacgaag atcatcacca cgctgccaag taatacgtac aacagtttgc tgaagctcat
     9301 cggcttgaag tcgggttcag aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac
     9361 cagtaatgcg ataattaacc ccgccccgca caggttgtag gtgactgaat aaccgaatct
     9421 atcagcgatc acaggggcca gcgataacgc tatcaacgag ccgatattga tcgacatata
     9481 gaacagggtg aatgcgccat caagccgcgg atctttcggc ggatagcact tcgaaagcaa
     9541 gctggctggg ttagctttaa acaggccgtt accgacagcg atagtcccca gggcgatgaa
     9601 aatcaggtca ggcttaagta gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag
     9661 tgctccaaga acaatggtgc gtttggtccc caacaggtgg tcgccgacat agccgccaat
     9721 ggaaatgagg ccatagacca gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg
     9781 cgagaatcca agctgtttaa cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc
     9841 gaatcgctcc cataactcga caaaaaagat catgaaaaat gggcgaggtt gctgcagcat
     9901 ccccatgggt gttgttgtat taatatttat taaccttcca ataccatcct gaaaagtatg
     9961 aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc agagattctt aatattgatg
    10021 cggtaaatag attaatacac tattacaaca gaaaataacc agctgatcac actaatgatg
    10081 gaaaatatga tggtgttaaa ttttgtattg gcaggagatt ttgattgcgt acgcagatcg
    10141 taggccagat aaggggttta cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc
    10201 ccgcatatgc gggcgttcag gaacacgtcg tggagggatg attattcttc ttcgtcgcgt
    10261 agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc
    10321 agtttgctcc agaagtcctg gtggtgacca caaaccacca gatccatatc gtatttcttg
    10381 attgcatcga ccagaacctg gcctaggtcg ccgctgccgc tcagggtttc agtgattgga
    10441 tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc
    10501 tgcatatcac ccagattcac atcaataagc ccggtgtata ggtcagagta gtttacatct
    10561 acgtggatca gagaaacttt cgcattgtag gggcgagcca tagagactgc tttctctacc
    10621 agaactttgc tttccgggga gagatcgact gcgatgagaa tgtgtttata agccatagtg
    10681 ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg ccgttacgtc
    10741 acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag
    10801 accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt acccgttaga
    10861 taaacaaatt ttcgacatga atattatatt gatagtggtt aaccttctgg aaaaaaaaca
    10921 acctgatctc ctacactatc tatagagccg ctcggatgtt ggtcaccaca aaagcggttt
    10981 ctccaggacc tggtgttaac tgattggcag cgtatcggga gcggtagccc cggggtggaa
    11041 ctccgcgggc aggtcgccgg ggaggaggta tgataagcac cgtcgcatta ttttgggctt
    11101 tatgtgtcgt ttgcattgtt aacatggcgc gctatttctc atcactacgc gcgttgttag
    11161 tggtactgcg taactgcgat ccattgctct atcaatatgt tgatggaggg ggctttttta
    11221 cctcacatgg ccaacccaac aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt
    11281 atcgcgatca tcacgatgat gagtttattc gccgctgtga gcgggtgcgt cggcagttta
    11341 ttctgactag cgcattgtgt ggtctggtgg tggtcagcct gattgcattg atgatttggc
    11401 attaagcact tcagcaataa taacgtataa aaaaagcggg ccagtaaact gacccgctcg
    11461 ttttggtgcg tacgattaca ggaactgcaa ggagaaccag tacagcccgc cggaaagtag
    11521 taccgcagcc ggaagggtaa acacccaggc catcagaata ctggttacgg ttttacgctg
    11581 taagccgccg ccatctacca ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt
    11641 ggaaaccggc atcccggtat aactcgccag gccgattgac actgccgccg tcatctgggc
    11701 agacatcccc tgagcgtagg tcatgccttt cttaccgatt ttctcaccga tagtcgttgc
    11761 cacacgacgc cagccaatca tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat
    11821 ccacaccggc gcatactcga tggtgctaag catgtcggac ttcagtttct tcaacaggcg
    11881 ctgatcgtca gcactcacac caggcatctt caccactttg tcgatagtgt cagaaacgca
    11941 cagcataatg cggcgcatct ggctacgttg atcaagcgac agcttgtcgt agctttccac
    12001 atcggtggtc aacatacctt tcaggcggtt gagcgcgtta atggtattcg acggatggca
    12061 gtggaactcc gcaggttgcg ttgcgccagc ttccggagcc ggtactaact gatcagcacc
    12121 ggtagcctgt ttgagcagcg caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc
    12181 atcacgggta cgggtgattt cgtagccagt ggcattcatg ttcaccacga agcctgctgg
    12241 cgcgacgcca atcaatacca acataaccag accaatgcct ttctgaccat cgttcgcgcc
    12301 gtgcgaaaac gccacgccga tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg
    12361 ctttttcttg ccgtctttct tttcacgctc cgctggggtc aggtggatac gggcgcgttt
    12421 cttggtgccg ctccagtagc gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc
    12481 gacaataggg gaaacgatca gagaaccgaa aatacttaat actttcggga tattgagtgc
    12541 atccaccact gacgtcccgg tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc
    12601 aatcagcgta tgagagctgg atgcaggtaa accaaagtac caggtaccca ggttccagat
    12661 aatcgccgcc agcaacatag agaacaccat ggcaaggcca tgagacgatc ccatattaag
    12721 cagcagatcc gtcggcagca tatgcacaat ggcataggca acactcagac cacccagcaa
    12781 aacacccaaa aagttgaaca ccgccgccat aaccacggcg agctgagaac gcatcgcgcg
    12841 ggtatagata acggttgcca cggcgttggc tgtgtcatgg aaaccattga tggcttcgta
    12901 gaacagcaca aaagccagtg caagcaataa taacagcccg gtatgcaaat ccaggccagc
    12961 aaacaaatgt agcataggac gttacgccat tttgaggaca tgaacgcggc gcattatcag
    13021 tgactttcgc ggcgcgggca aagtgaaata tagacttttt ttgatttgcc tcctgtatgg
    13081 atttcactca aaaaataatt atcttatata attcaggcaa atacttcctt ttagtaatat
    13141 tgatgctggt gcgaccactg aggaatcttt acaattcacg cccgtttttt ctaagaggag
    13201 cgcaacgtgg aaaggtttga tgccattatt ataggcgctg gtgcggcggg tatgttctgt
    13261 tctgcgctgg caggtcaggt aggacgccgg gttctgctga tcgataatgg taaaatacta
    13321 gggcgcaaaa tccttatgtc tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa
    13381 ccaggcgctt atctgagcca gaatccgcat ttttgtaagt ctgcactcgc acgttttacc
    13441 cagtgggatt tcattgatct ggtcaataaa cacggtatcg cctggcacga gaaaacgtta
    13501 gggcaactct tctgcgatga ctccgcgcag cagattgtcg acatgctggt ggatgagtgc
    13561 gagaagggca atgtaacctt cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa
    13621 acaggcttca cgcttgatct gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg
    13681 accggtgggc tgtcaatgcc ggggctgggc gcatcgccgt ttggttataa gattgccgaa
    13741 caatttggcc tcaacgtgct gccgacccgc gcgggtctgg tgccattcac tctgcataaa
    13801 ccgttgctcg aagagttaca ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct
    13861 gaaaacggca ccgttttccg tgagaactta ctcttcaccc accgcggctt atctggaccg
    13921 gcggtgttgc agatttcaag ctactggcaa ccgggtgaat ttgtcagtat caatctgcta
    13981 ccggatgtgg acctcgaaac cttcctgaat gagcagcgta acgcacatcc gaatcaaagc
    14041 ctgaaaaaca cactggcggt tcatctaccg aagcggttgg ttgaacgctt acagcaactc
    14101 gggcaaatcc cggatgtttc gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt
    14161 agcacattga ccgactggcg cgtacaaccc aacggcactg aaggctatcg cactgccgaa
    14221 gtgacgctcg gcggcgtgga caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa
    14281 gtgcctgggc tgtacttcat cggcgaagtg atggacgtta ccggctggct ggggggctat
    14341 aacttccagt gggcgtggtc gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg
    14401 aaataaccat gacgttttcc cgtcagccgt ttgtatatgt cgttattaag tcattttggt
    14461 acggttctgc atccgacatt ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat
    14521 tgccgttgtg gttattttat agtgtattta ttctcagaga atatatatta ctgaaataaa
    14581 aaacacaaag aataaccaaa ggaaattatt tgaaacgtaa aaggtatata tttttaatct
    14641 gtaaaattta aaatcacctc ctctggattg aatcaacttg ctctatttaa gacgattgtt
    14701 tttacatttt caccacctgt atcaggtggt gtttttttat tggcggcaat gcctttatct
    14761 tatttttatt acgttgtaaa tatggctatt cctaaatgaa atttgatttg ctcagtttat
    14821 ttgtgatgtc aggggagtgc ttgccatgag agtcgtgata ttattattca tcatt
//
LOCUS       DAJVRP010000097        14566 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVRP010000097 DAJVRP010000000
VERSION     DAJVRP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.097"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..581
                     /locus_tag="OQN12_003176"
     CDS             <1..581
                     /locus_tag="OQN12_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCS1655412.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDYFVVI"
     gene            complement(635..1357)
                     /locus_tag="OQN12_003177"
     CDS             complement(635..1357)
                     /locus_tag="OQN12_003177"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1655413.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1359..2530)
                     /locus_tag="OQN12_003178"
                     /pseudo
     CDS             complement(1359..2530)
                     /locus_tag="OQN12_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="OQN12_003179"
     CDS             complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="OQN12_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCS1655414.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="OQN12_003180"
     CDS             complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="OQN12_003180"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCS1655415.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="OQN12_003181"
     CDS             complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="OQN12_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCS1655416.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="OQN12_003182"
     CDS             complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="OQN12_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCS1655417.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="OQN12_003183"
     CDS             complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="OQN12_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS1655418.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="OQN12_003184"
     CDS             complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="OQN12_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1655419.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="OQN12_003185"
     CDS             complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="OQN12_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1655420.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="OQN12_003186"
     CDS             complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="OQN12_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1655421.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="OQN12_003187"
     CDS             complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="OQN12_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1655422.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13620..>14566)
                     /locus_tag="OQN12_003188"
     CDS             complement(13620..>14566)
                     /locus_tag="OQN12_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1655423.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3757 a         3717 c         3317 g         3775 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gattatttcg tagttattta aataatgaga acaggccgga
      601 gcgtaattca tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag
      661 cggctttatc agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat
      721 cttcggtaac cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga
      781 caattatcag caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt
      841 caggcactaa cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc
      901 cctggatcgg gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac
      961 aaaaatgacc atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca
     1021 gtttctgatc atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga
     1081 tccccgcagg ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt
     1141 tgccgcgaaa gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac
     1201 ctttaaccag ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg
     1261 ccaggttaac acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc
     1321 tcatttcctg gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc
     1381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag gccgggatat
     1441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
     1501 ttgccggtta cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta
     1561 atagctgccg gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta
     1621 acaccactga atgtggccag ccaaagcttt ccagatgatt cattccggaa accagcgcct
     1681 ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc ggcatcttta cggacactaa
     1741 aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat
     1801 tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga ataatgccct
     1861 tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca
     1921 gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt ccggtatcac
     1981 caaaaccgtg atagaaatag agcactggca aaggctcgcc catgccggtg tatcctggcg
     2041 gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa gttatggcga
     2101 tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc agaatcatac
     2161 tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg ctgcgtacac
     2221 catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg cgccacgacc
     2281 agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca
     2341 ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggcat
     2401 tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct ggcatatccg
     2461 ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt
     2521 ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt cagcctgggc
     2581 accgacagca aactgggcgt ctttattgtc ttcgaaggtg aagccatcca gttcgttttc
     2641 cagggctttg atatacgtgg cgtagaaacg aatttccaga cgcgaggtca acatactggt
     2701 attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa atttattact
     2761 attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg
     2821 gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag agtgtgcgcc
     2881 tgtttcgtag ctataaatat tgttaccgaa ggagtaaaca atcgcgttag ccacaataaa
     2941 atgatcgccg atataggctt cgccctgcga ggtcagacga actgctgttc cgccggtgtg
     3001 atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat aacggccaaa
     3061 gttcctggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacctttatc
     3121 aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat aatacccgtt
     3181 attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac
     3241 cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa tagtatgggt
     3301 attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag
     3361 cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac ccgctgcagc
     3421 atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg caccgtgttt
     3481 acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat
     3541 atcggagaac tgcataaagt tctcgccacc ggcattatcg ccaaaccagc cggtagagta
     3601 ctgctgacca acgttaccat ctatcatcac aacggcatca acccgtttgc cgttttcgtt
     3661 gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc
     3721 cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc aaccagaacg
     3781 gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga aatctttcag
     3841 agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat ttttgaccag
     3901 tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat acccttgatc
     3961 attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct cttcatcttt
     4021 atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact caaggcgttg
     4081 ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg ccattaataa
     4141 gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga
     4201 attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttattggtt
     4261 atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg aagcttttct
     4321 tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa ataaagccgt
     4381 agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt
     4441 accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta
     4501 aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttacgc
     4561 ctaatccatt ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca
     4621 gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat
     4681 tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa
     4741 cacaaccagt catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg
     4801 cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc
     4861 cgggatattg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca
     4921 tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca
     4981 tattgccgat tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg
     5041 caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc
     5101 ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg
     5161 ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga
     5221 ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg
     5281 ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa
     5341 acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg
     5401 caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt
     5461 aacggtaata aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt
     5521 ccatttttcc cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt
     5581 ccagccaggc accttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg
     5641 gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac agcggttcac
     5701 ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga
     5761 ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa
     5821 tcagccgatc gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc
     5881 cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact
     5941 caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac
     6001 gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg
     6061 tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc
     6121 cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct
     6181 gacgtttact ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa
     6241 tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct
     6301 gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
     6361 tgatggtggc ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca
     6421 ggttgacgcc atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc
     6481 ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
     6541 cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
     6601 agttattgat acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc
     6661 agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac
     6721 cggcggcaat catttcgctt atccaggttg atagcggccc caccagcaga aaggtgacgg
     6781 gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc
     6841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
     6901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat
     6961 ccgctttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa
     7021 tcaccatggc agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta
     7081 tcgggaagaa cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt
     7141 gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg
     7201 ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
     7261 gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
     7321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac
     7381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
     7441 tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt
     7501 tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc
     7561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
     7621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata
     7681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt
     7741 gaaattttac gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat
     7801 gcttgcgggt aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa
     7861 gcatgttcaa gaatacgcca agataaaaac ttcagatgtg taaccagtcg ctgataactc
     7921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc
     7981 attaactgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
     8041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
     8101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac
     8161 agcaatacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
     8221 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
     8281 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
     8341 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
     8401 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
     8461 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
     8521 ataaacaaaa aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga
     8581 atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa
     8641 ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct
     8701 cgcagttatt aactttatgt aattttagga atttataaag ttatatataa caaatcccaa
     8761 taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag
     8821 tttatccagc tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat
     8881 aaactcacaa atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag
     8941 cgcggtaagt acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg
     9001 aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc
     9061 gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg
     9121 gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc
     9181 agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact
     9241 gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac
     9301 gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg
     9361 cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc
     9421 caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg
     9481 gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa
     9541 cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg
     9601 ttgctgaact caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca
     9661 gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc
     9721 agctcttcaa tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc
     9781 agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca
     9841 gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg
     9901 gtcaatgccc actgcacgcg ctcgtccatg tcggcacagg agagcttctc aaacagacga
     9961 acgccaaagg cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc
    10021 atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca
    10081 tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag
    10141 gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt
    10201 ttggcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa
    10261 ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca
    10321 tcgtacctga tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc
    10381 gcatccggca aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt
    10441 cagcagcagg acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc
    10501 cgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat
    10561 catgtccgtg ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc
    10621 ggcaatacgg gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag
    10681 cgtaatcgcg gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag
    10741 gctgtccggc accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa
    10801 cagcgccagc gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat
    10861 ggtgtagaca aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa
    10921 gcgaatcact tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat
    10981 aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag
    11041 ggcgttcgcc agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc
    11101 cagcgacata ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa
    11161 caggccgaag gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg
    11221 acgcgcctgc attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat
    11281 agccattagc gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg
    11341 aggacgatga aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc
    11401 agaccggatt ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc
    11461 atgtacagcg aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg
    11521 gtttcaccga gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg
    11581 aacggaagaa cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac
    11641 tctttcatca tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga
    11701 ataatcatga tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca
    11761 gagaacagcg cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag
    11821 taaacggcga acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga
    11881 atggctgcca gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc
    11941 tcagtcagga acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac
    12001 gaagtcacca acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc
    12061 cactctttgg tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg
    12121 atcagagaga caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc
    12181 agcacgctga aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca
    12241 gccataagtt actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg
    12301 cgtaaaacac cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt
    12361 taatattggt cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt
    12421 aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca
    12481 cctctgtgcc ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg
    12541 taataggcca tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc
    12601 agtctgcacc ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat
    12661 cagcggagat cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat
    12721 aaccaattgc acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc
    12781 ccagaccgat cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt
    12841 tcactttcgc caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta
    12901 ctacagcaat gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat
    12961 catcccactt cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca
    13021 gttcgccaga cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg
    13081 ggaactggaa cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac
    13141 caaaatcaac ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt
    13201 agttaacttt attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg
    13261 cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca
    13321 tcgataaggt cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc
    13381 ctgggaggat tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt
    13441 gacagttata tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta
    13501 ctgattttaa atgtgaagat gaaaatccca catgccatct ttttgtcatg atgcatttaa
    13561 aagtgataaa gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat
    13621 tagcgcacaa tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga
    13681 gctccagtaa ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac
    13741 actgttgatt tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta
    13801 tttttgtcgt aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc
    13861 gtattgggtt cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg
    13921 ttttcacact caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca
    13981 acagatgaaa aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta
    14041 tttatttcgc aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc
    14101 tgtgagtaat ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt
    14161 tcaggaataa tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta
    14221 gccccactgg atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac
    14281 gggttgttag catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata
    14341 gcaatcccaa catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca
    14401 gcataagtta atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg
    14461 ttaccagtat cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta
    14521 aatgtcgttc caaccttatt ttcgttcgcg gtaataagat atcccg
//
LOCUS       DAJVRP010000098        14515 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVRP010000098 DAJVRP010000000
VERSION     DAJVRP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.098"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(113..379)
                     /locus_tag="OQN12_003189"
     CDS             complement(113..379)
                     /locus_tag="OQN12_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS1655424.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="OQN12_003190"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="OQN12_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1655425.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="OQN12_003191"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="OQN12_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="OQN12_003192"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="OQN12_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1655426.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="OQN12_003193"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="OQN12_003193"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1655427.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OQN12_003194"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="OQN12_003194"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1655428.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OQN12_003195"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="OQN12_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1655429.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="OQN12_003196"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="OQN12_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OQN12_003197"
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="OQN12_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS1655430.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12641..14095)
                     /locus_tag="OQN12_003198"
     CDS             complement(12641..14095)
                     /locus_tag="OQN12_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCS1655431.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14515)
                     /locus_tag="OQN12_003199"
     CDS             complement(14092..>14515)
                     /locus_tag="OQN12_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1655432.1"
                     /translation="FSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGTSLVSLVPVSE
                     LWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATG
                     NFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3873 a         3663 c         3429 g         3550 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaactgctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caagatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgctg
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgcac gaaccacggc agcctgacgg ctgcaaattg aaaac
//
LOCUS       DAJVRP010000099        14475 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVRP010000099 DAJVRP010000000
VERSION     DAJVRP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.099"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(337..582)
                     /locus_tag="OQN12_003200"
     CDS             complement(337..582)
                     /locus_tag="OQN12_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655433.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="OQN12_003201"
     tRNA            complement(1227..1302)
                     /locus_tag="OQN12_003201"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="OQN12_003202"
     CDS             complement(1408..2115)
                     /locus_tag="OQN12_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1655434.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="OQN12_003203"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="OQN12_003203"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1655435.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OQN12_003204"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OQN12_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1655436.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OQN12_003205"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OQN12_003205"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1655437.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="OQN12_003206"
     CDS             complement(7299..7574)
                     /locus_tag="OQN12_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1655438.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OQN12_003207"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OQN12_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1655439.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="OQN12_003208"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="OQN12_003208"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1655440.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="OQN12_003209"
     CDS             9534..9860
                     /locus_tag="OQN12_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1655441.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="OQN12_003210"
     CDS             10044..10763
                     /locus_tag="OQN12_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1655442.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="OQN12_003211"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="OQN12_003211"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1655443.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13063
                     /locus_tag="OQN12_003212"
                     /pseudo
     CDS             12102..13063
                     /locus_tag="OQN12_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13164..>14475)
                     /locus_tag="OQN12_003213"
     CDS             complement(13164..>14475)
                     /locus_tag="OQN12_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1655444.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKL
                     ILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLI
                     VGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCS
                     ISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3795 a         3472 c         3721 g         3487 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct atttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaagtggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaataaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacttggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    13021 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tagaaaaaag ataccgaaca
    13081 actgcggcgc aatggggtga ttaagtaact catgttaagt gcctttgccg gataatataa
    13141 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    13201 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatagcagc
    13261 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    13321 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    13381 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    13441 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    13501 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    13561 tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc
    13621 gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg
    13681 taaatcagca ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg
    13741 gtaaaaatgc cgcccagaat gccgcccaga atgccgccca tcacaatgaa caccagaagc
    13801 aaacttggta aggcatccag aaacgcttta caggcctgag caaaggttgc tctttctgaa
    13861 agcgggtagc cacggcgttt agcaataatg ccgcagacta tcattaccgc aagacccatt
    13921 aagatccctg gcaaatatcc cgccataaac agcgatgcta cagaaacgcc gccagctgtc
    13981 agggaaaaca caattaacac gtttgagggc ggtatcagta atccagtaat acaagatgag
    14041 acgtttacag cggtagaaaa tgccggatca taaccttctt tggtctgaat gggattcagt
    14101 gtgccaccaa cggcagcagc tgccgcaacg gctgaaccag aaatggaacc aaacatcata
    14161 ttcgccagga cgttcacatg accaagcgaa ccaggaacgc ggccaaccat tacctgggcg
    14221 aggttaatca aacgtatcgc aataccgccg ctgttcatca gtgttccggc aaagatgaaa
    14281 aaggggattg ccaacagggc aaagttatcc agtccattag ccagtcgttg cgaaacagtg
    14341 attgccgcga tatcccaggg aaacatcaaa agcattgaag ctacagtggc aataccgatt
    14401 gagaacgaaa tgggcacacc aataaagacc agcacaaaaa aacagccaaa cagtgtaatc
    14461 gcgatataac tatcc
//
LOCUS       DAJVRP010000100        14249 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVRP010000100 DAJVRP010000000
VERSION     DAJVRP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.100"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..104)
                     /locus_tag="OQN12_003214"
     CDS             complement(<1..104)
                     /locus_tag="OQN12_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCS1655445.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="OQN12_003215"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="OQN12_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCS1655446.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1353..2003
                     /gene="csgD"
                     /locus_tag="OQN12_003216"
     CDS             1353..2003
                     /gene="csgD"
                     /locus_tag="OQN12_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1655447.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2008..2397
                     /gene="csgE"
                     /locus_tag="OQN12_003217"
     CDS             2008..2397
                     /gene="csgE"
                     /locus_tag="OQN12_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCS1655448.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2422..2838
                     /gene="csgF"
                     /locus_tag="OQN12_003218"
     CDS             2422..2838
                     /gene="csgF"
                     /locus_tag="OQN12_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCS1655449.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2865..3571
                     /locus_tag="OQN12_003219"
                     /pseudo
     CDS             2865..3571
                     /locus_tag="OQN12_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3704..4195)
                     /locus_tag="OQN12_003220"
     CDS             complement(3704..4195)
                     /locus_tag="OQN12_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1655450.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4297..4851)
                     /gene="ycdY"
                     /locus_tag="OQN12_003221"
     CDS             complement(4297..4851)
                     /gene="ycdY"
                     /locus_tag="OQN12_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1655451.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4875..5612)
                     /gene="ycdX"
                     /locus_tag="OQN12_003222"
     CDS             complement(4875..5612)
                     /gene="ycdX"
                     /locus_tag="OQN12_003222"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1655452.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="OQN12_003223"
                     /pseudo
     CDS             complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="OQN12_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6840..6927
                     /locus_tag="OQN12_003224"
     tRNA            6840..6927
                     /locus_tag="OQN12_003224"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6874..6876,aa:Ser,seq:gga)
     gene            complement(7077..7358)
                     /locus_tag="OQN12_003225"
                     /pseudo
     CDS             complement(7077..7358)
                     /locus_tag="OQN12_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7640..8644)
                     /locus_tag="OQN12_003226"
     CDS             complement(7640..8644)
                     /locus_tag="OQN12_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCS1655453.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8716..9276)
                     /locus_tag="OQN12_003227"
                     /pseudo
     CDS             complement(8716..9276)
                     /locus_tag="OQN12_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9361..9558)
                     /locus_tag="OQN12_003228"
     CDS             complement(9361..9558)
                     /locus_tag="OQN12_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655454.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9570..10061)
                     /locus_tag="OQN12_003229"
     CDS             complement(9570..10061)
                     /locus_tag="OQN12_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655455.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10058..10432)
                     /locus_tag="OQN12_003230"
     CDS             complement(10058..10432)
                     /locus_tag="OQN12_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCS1655456.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10479..10853)
                     /locus_tag="OQN12_003231"
     CDS             complement(10479..10853)
                     /locus_tag="OQN12_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCS1655457.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11016..11237)
                     /locus_tag="OQN12_003232"
     CDS             complement(11016..11237)
                     /locus_tag="OQN12_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCS1655458.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11300..11776)
                     /locus_tag="OQN12_003233"
     CDS             complement(11300..11776)
                     /locus_tag="OQN12_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCS1655459.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11792..12265)
                     /locus_tag="OQN12_003234"
     CDS             complement(11792..12265)
                     /locus_tag="OQN12_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCS1655460.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12607..13428)
                     /locus_tag="OQN12_003235"
     CDS             complement(12607..13428)
                     /locus_tag="OQN12_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCS1655461.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13528..13701)
                     /locus_tag="OQN12_003236"
     CDS             complement(13528..13701)
                     /locus_tag="OQN12_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCS1655462.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13821..>14249)
                     /locus_tag="OQN12_003237"
     CDS             complement(13821..>14249)
                     /locus_tag="OQN12_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655463.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3641 a         3489 c         3412 g         3707 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tatttttata
     1021 tgtattatta gtaagttagc accatttgta tgatttttta aaattgtgca ataaaaacca
     1081 aatgtacaac ttttctatca tttctaaact taataaaacc ttaatgttaa cattttaata
     1141 taatgagtta cattttgtta catgtttaac acttgattta agatttgtaa tggctagatt
     1201 gaaatcagat gtaatccatt agttttatat tttacccatt tagggctgat ttattactac
     1261 acacagcagt gcaacatctg tcagtacttc tggtgcttct attttagagg cagctgtcag
     1321 gtgtgcgatc aataaaaaag tggggtttca tcatgtttaa tgaagtccat agtattcatg
     1381 gtcatacatt attgttgatc actaaacctt ctttgcaggc gacagctctc ttgcagcacc
     1441 ttaaacaatc gctagcaatt acaggaaaat tacataatat tcaacgttct ctggacgata
     1501 tctcttcagg ctctattatt cttctggata tgatggaagc ggataaaaag cttatccatt
     1561 attggcagga tactttgagc aggaaaaaca acaatatcaa aatattgttg ctaaatacgc
     1621 ctgaagatta cccgtaccgc gacattgaaa actggcctca tatcaacggc gttttttatg
     1681 ccatggagga tcaagaacgt gttgtcaatg ggttgcaagg cgtcctgcgt ggcgaatgct
     1741 actttacgca aaagcttgcc agttacctga ttacacattc tggtaactat cgttataaca
     1801 gcacggaatc agccctcctt actcatcggg aaaaagagat cctgaataag ctgcgtatcg
     1861 gcgcgtctaa taacgagatc gctcgttcgt tgttcatcag cgaaaatacg gttaaaacgc
     1921 atctttataa tcttttcaag aagatagccg taaaaaaccg gacacaagcg gtttcctggg
     1981 caaacgataa cctcaggcga taaagccatg aaacgttatt tacgctggat tgtggcggca
     2041 gaatttctgt tcgccgcagg gaatcttcac gccgttgagg tagaagtccc gggattgtta
     2101 actgaccata ctgtttcatc tattggccat gatttttacc gagcctttag tgataaatgg
     2161 gaaagtgact atacgggtaa cttaacgatt aatgaaaggc ccagtgcacg atggggaagc
     2221 tggatcacaa taacggtcaa tcaggatgtt attttccaga cttttttatt cccgttgaaa
     2281 agagacttcg agaaaactgt cgtctttgca ctgattcaaa ctgaagaagc actaaatcgt
     2341 cgccagataa atcaggcgtt attaagtacg gacgatttgg cgcatgatga attctaaata
     2401 aaaaattgtt cggaggctgc aatgcgtgtc aaacatgcag tagttctact catgcttatt
     2461 tcgccattaa gttgggctgg aaccatgact ttccagttcc gtaatccaaa ctttggtggt
     2521 aacccaaata atggcgcttt tttattaaat agcgctcagg cccaaaactc ttataaagat
     2581 ccgagctata acgatgactt tggtattgaa acaccctcag cgttagataa ctttactcag
     2641 gccatccagt cacaaatttt aggtgggcta ctgtcgaata ttaataccgg taaaccgggc
     2701 cgcatggtga ccaacgatta tattgttgat attgctaacc gcgatggtca attgcagttg
     2761 aacgtgacag atcgtaaaac cggacaaacc tcgaccatcc aggtttcggg tttacaaaat
     2821 aactcaaccg atttttaagc cccagcttca taaggaaaat aatcatgcag cgcttatttc
     2881 ttttggttgc cgtcatgtta ctgagcggat gcttaaccgc cccgcctaaa gaagccgcca
     2941 gaccgacatt aatgcctcgt gctcagagct acaaagattt gacccatctg ccagcgccga
     3001 cgggtaagat ctttgtttcg gtatacaaca ttcaggacga aaccgggcaa tttaaaccct
     3061 acccggcaag taacttctcc actgctgttc cgcaaagcgc cagggcttac aaaacctgct
     3121 taacgagcgc aaggttattc gtgcggcaca agaaaacggc acggttgcca ttaataaccg
     3181 aatcccgctg caatctttaa cggcggcaaa tattatggtt gaaggttcga ttatcggtta
     3241 tgaaagcaac gttaaatcgg gcggtgttgg ggcaagatat tttgggatcg gtgcagacac
     3301 gcaataccag ctcgatcaga ttgccgtgaa cctgcgtgtc gtcaatgtga gtaccggtga
     3361 gatcctctcc tcggtgaaca ccagtaagac gatactttcc tatgaagttc aggccggggt
     3421 tttccgcttt attgactacc agcgcttgct ggaaggggaa gtgggttaca cctcaaacga
     3481 acccgttatg ctgtgtctga tgtcggctat cgaaacaggg gtcattttcc tgattaatga
     3541 tggtatcgac cgtggtctgt gggatttgta aaataaagca gaacggcaga atgacattct
     3601 ggtgaaatac cgccatatgt cggttccacc ggaatcctga cagataaaaa tgccggaatt
     3661 aataatccgg catttactgt ttaagcgaaa gcgtgagtgc ccctcacgct ttatttttga
     3721 tttcctgctc acggtgagcc gtctttgcac tacgcgccgc cagccacagg ccgctgtttt
     3781 tcattgcgta accaaatacc agcccaagcg ccagcgaagg taacaccagt ttccagtcac
     3841 cctgcccggc aaacgtcgcg catgcgccta taaacgtccc cggaacaaat gaaagcagca
     3901 gctgtttggc ctgaatacac atcagaaacg cgacaatacc ggttatgaca taaccgagaa
     3961 tttccagatg tggtgccagt gcactaccgt aaataatgac catcgcccac accacgccac
     4021 taagcagggt tgcagcggag atcgccagcc ctttcagccc accttgcggg caagcaaagt
     4081 aggccgtaca gcccaggaag cccgcccagc ttagtaagcc cagggaaaca gccacccatc
     4141 cccagatacc ggagagaatg cctgttgtga ttgcaataga gagaagtatg ttcatgccgc
     4201 gcatgatagc agaaaacgcg gccatgaatg agatcgagca cacattttat gcaacaacaa
     4261 attatctgtt gcagttatga gtgacgatga tcacatttac tcttcagaat cttcttccag
     4321 ctcgtcccac attgcactaa tggcatcacg ggttagcggt gccatggtgc gccagaaagg
     4381 cgtggttgca tgggcctcca ctttgccgag gaacgcacca caccagggta acagatactc
     4441 actgaacagt gtttccagtg cttcgctctc atcttccgtt gactgatctt ccagccagga
     4501 agccgcaagc agcaatgtgc cgatgtgatc ggctggcgta tccgccagtg gcatccctcg
     4561 ttcggaaaga aaagcgcgca cttccgcttc cgttgcgtcc tcaacccatg cgctacgata
     4621 tggcggcacg gcacattcat cgccgataaa caacgcattg taatcggcag agacttgcgt
     4681 catatcacag cttttctgta aacgcgtcag taactcatcc tgctccagtg gccagttcgc
     4741 agccagtttc ccctcacgaa tcagagtaaa cagcggcacc agtaaaggat cttgcggttg
     4801 gcggtaatag agcgaaccca gcacacgaca gaggatagaa aactcgttca ttaatatatt
     4861 tccattacta gtaattaaag gtctgcaaat tccgcaatcg gtgccatacc gcgagattca
     4921 aggaagttca gtaagcggcg cggagaaaca ttcaaaatgc gctctagcgg aaaatctacc
     4981 gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg taaacgcggt gtgagaatcc
     5041 gagcctaatg ccacccaacc tccagcatca cgtaccgctg cagctaccgc acgacagttg
     5101 tcttcactgc ccttacgtga gtgtaaaaat gaggaattat tgatttccag cgccacctga
     5161 tgtttcgcgg ctgcttcagc aacggcttta acatctattt catatttcgg atttccggga
     5221 tggcttatta tatgcacatt gccgctggcg atagtggaga tcatcgcttg tgtattggtc
     5281 gctttgtcat gtggcgcaaa aaccggctca tgaaaaccgg caataatcag atccagcgag
     5341 tcaaacattt taccgctgca gtcaatttca ccatcaacat ttttaatgtt agcttcaatg
     5401 ccgcgcagga tccctacccc atcaaccact cgcggccaga tacgcatgtt aatgaagtgc
     5461 cagtgatgcg gcgcatcttc catatccggg ccatgatcgg tgatcgcaaa aagtttaatg
     5521 cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc tatatgcatg tgtgctggca
     5581 acggtatgca tatgaaggtc gacgggatac ataactctct cctgtattca attcccgcca
     5641 aggatagcag gaatcctgat gctttattag tagccacgtg cgcggtcgac ttgcccgcag
     5701 accctctccc ctttttcgag ctgggcaata gtgcgagaaa tgtactccac agcttcagcg
     5761 ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc gtggatgttg ccagagcgga
     5821 ctttcaggcg gtaagggttc acgattaaaa acatccagca ttgcgccttt aactttcccg
     5881 ctatccagcg ccgcgagcag gtcatcttcc acaacatgaa caccacgcgc caggttgagg
     5941 agatacgcgc catcaggtaa tttttcgagt aattgttgat taataatgcc gacggtttca
     6001 ggggtattcg gtaacaaatt aatcaatacc cgacattggc tcagaaatgc agacagttct
     6061 tcccgtccgg caaagctttg cacgccaggc cacgatttac gggttcgact ccagcaacgc
     6121 agcggaaagc gctaggtttg cagactctga gcaactttac agcccagtac gcctgcgccc
     6181 aaaatgccga tggtaaaatc ttcccgatga tattcaggca gcggttgcca atgcgaacta
     6241 ttttgctgga tgcgataatc gtcaaaacgt cgaaaccaat gcagcacctg actgacagca
     6301 tattcctgca tttgctcgcc cataccggta tcttccaggc gaaaaagtgg aacagaaggg
     6361 ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt
     6421 gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa caggaggatg ccagactaaa
     6481 acataatcag cagagtcatt atctccgctt ttccatgccc tgactcttgc ctgaggaata
     6541 gctttgcgca gtgcctcaat ccaccattgg gtatcgaacg ttgggtgata aaagatgata
     6601 tccatactga ctcccgaaaa gcgttgtgcg aaatttattc gcacttatcg ttatgatcta
     6661 caaaggccac cagcataaca aatccgtggt cggtggcaaa aaaagcagat ttcgcttatt
     6721 aaaaccacac attgattgaa tgtttgaata aacgcgcgat tttttcaaaa agtttgttga
     6781 cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg
     6841 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
     6901 ggggttcgaa tccccccctc accgccagat tataagtgca tgttattgtt taacatgcgc
     6961 ttttttattt ttccttccag acagtatgcc atcgggtatg ccatctgaaa ccggtttata
     7021 tgccccgcga tcatatctta tcggacaatt tccgatcttt gagcatactg gcaaagctat
     7081 actgccagca tatcaggata gtgttttgcg ataatatcat tcagtctgtc aaccagttct
     7141 ggttttctga acgtgatatg cgcggagccc ttcttaaagt atctgatgct gaacatctca
     7201 tcttcatagc tctctttacc ctgaacagac tgaatgtgat catccagacg aagggtgatg
     7261 tttccccggt tgtcggggat tggtttaccg ctgaacaggt gcaacattct ttccagatcg
     7321 gcgagtcggt cagcctgttg tccggtgata agatgaaatc tatggtcacc cccgtttttg
     7381 caacactgat ttttgattaa tggttggctt gcttaaatct atccggcgtc tctgtgggca
     7441 aggatgcccg cgccccgatg agttccgcac ctgataatcc taaaaatccc ctcggcttca
     7501 gtgagccatt tcatctgtta ggtttttaat caggtcgatt tgccgttcat gtcatcaata
     7561 atcagtcttc gcaaaaccag aaaggtaact tgtatcttat tgtttaaaat aaactaattg
     7621 agttatatca gcatctatat caggcctgat attctttttt ctgagtagtg attgcccagg
     7681 caattcgagc cagtttattt gccagagcgc aggtaaccac attcgaatgc ttcttgttga
     7741 gttgctcccg aacccactca gcaagttttc catgcttgcg ttcaagtcgc attatgaatg
     7801 agcgggcaca ctgaaccagt aaccggcgta aatttttatc acctcgctta ctgataccca
     7861 acagagtcga ttttcctcca gtgctatact gccggggaac aagtcctgtg gaagctgcaa
     7921 aatccctgct gcatgagaac tgtttcccgt caccaagttg cgacgatagt aagctggcag
     7981 taatcggacc aacaccagga attgtcataa tgcgctgagc ggtatcatca gcgttgattg
     8041 actgacttaa ttctgattcc agctctgcta tttgctcaac aaggtaaaag tagtgggtat
     8101 gcaacctcac caaaaggcgg ctcagatatt cggggatttc gtgttcagcc aacacgagag
     8161 acaaccgttt tattacagca tcacctatcg gcaggctgat tccaaattcc agcaagaaac
     8221 cgtggatctg gttggttgtt ttcaccttat ctctgatgag cgattctctg acccggtgta
     8281 acgccctcat ggcctgctgg gtctcagttc ttggctggac aaaacgcatt gatggtcgtg
     8341 aagccgcttc gcatatggct tctgcatcaa caaaatcatt cttattactt ttgacaaacg
     8401 ggcgaacgaa ctgtggagaa atcagcttag tttcatgccc aaaatcagag atacagcgag
     8461 ccataaagtg tgcacccgca caggcttcca ttacaacaac tgagggcgga catgtcgcca
     8521 gaaactgcat cagtttagta cgggtaaatt ttttacgcag aagagcctta ccagacttgt
     8581 cttgacaatg aatgtggaag gagtgctttc cgagatcgat accaataagt gtgatgttct
     8641 gcatgatggt ctctccgtta aagggattta ccttaacagc gtactgctgt taaggtgggg
     8701 gtgaccatat cattatcccc accggtccca tctcaccaga ttgttgacga taattttact
     8761 gccaaattta caggggcaat tggttttgta attccagttc agccctctga agacgttgat
     8821 cacccctcgc tcaaacacct catccttatt ctggtgcagt tgttcaaatg tgctcagaat
     8881 gttcgcttcg ctgatttccg gaaaattatc gtactccagt gaccggtacc atgtatcacg
     8941 ggcctgcgta tccattaatg acagcatccc ggaacgttgc atcaggtcac gccagatttc
     9001 gcgatcaaga ttccgggtaa tggctttcat tgctgtttcg ggtttctcca tcagccagca
     9061 gccgcagcga aaatcctgtt tcatggccca gtccggggca gttttacctc cgatgctttc
     9121 cgtcagcacc gagatttcag ccagttgtcg gataagtgtg tcgatttgca acagtgtact
     9181 ctttcgtaca gccagaatgt ggcgaatgct ggttgagcag ataacgtccg tatgcccgtt
     9241 gaccacttcc ggacgggaga ttgtgatatc tgacataatc gatattcctt tttgcagata
     9301 aatgaaaacg ccagccctgt gcaggctggc gtcagaggaa tgaagtctgg tgagtgagct
     9361 tcaccggttc tggtgcagaa gctgcagatg acgcagggta cggacatcct cccgggcctg
     9421 ttcgatacag ggcaacagtg ctgctgaatc cgttttatcc tcatcgttgt caaagataat
     9481 tcccgatccg cagtcgatat tgtcctgcag ccacgcaatc agaatatcca gcgctgtttc
     9541 cgtggttaat gatttcatgt tgttcctggt taccagtcga aagtgggtaa acccggcagg
     9601 cattcagcgc tggcatccag atgaatgaga ctgacgccat aacgccggat gagtgtgacg
     9661 accagacgac ggaacgtttt tgacagcccc aggcgtttaa gccgcagaac cgggtatgac
     9721 cacgcatcgg tacgtaacag atatccgcta cctgtaaaat gaatccattc tgattcaccg
     9781 aagtcactgg tctggtgtga cagcgagtac aaccaggcgt tgtccgtttc cgtgatatgt
     9841 gcggtactgc agcgtatgcc ggtaagggta gcaaatggtg gcgtgcattc tgctgaggtc
     9901 tggtcagatt catcaacaac acgaagggag tagccgttat cgcagaccat gttaatcagt
     9961 tcagcgagct caataccatc gacgtcaacg acaatgcgcc ctatgttcag tgcctgtacg
    10021 ttaacgctgt cggcttccag tgtcagggcc agtttcatca tttcgcctcc ggatacttac
    10081 ccagggtaat gttatttacc gttctgtaat tgtcgcgggt catcaggccg gtcgcccggc
    10141 gagcccggag gatatcaata ctgtttatta actgagagcg gggacaggtg ctgaatcccg
    10201 gctggtcggt acgcaccagc gcgtattttt ccacgagaaa attaactgca tcacacagtg
    10261 aaatgcctgc ctcaatatgc tgctcaatca cacgttcatc gacaaacggt gtgtcattca
    10321 gtgtgaggcc ataatgctgg tccagcagtc ggatgagcag tgtctgccag atggtgacgg
    10381 gagacgggca gcgagatacc tcccgtacat gtgtgtcgga taatgttttc atcttaagtt
    10441 ctctgtaact ggtagtgcta aaggtgagag cttcggggtt atgaggtggt tgcgggtgct
    10501 ggtgtcggat aaacagccag ataaacgtaa ccacaggagc cgagggtgtc ggcttcgcag
    10561 gtcagccctt ttgcatacag cgtgacggta tgctgatatc ggggattcag ttcaccgctg
    10621 gtgagcatga gttccagttg tttcatcagc agcggaaagg cctggtccag atggtatgca
    10681 tctgcgtcgc tgaagcggcc tctgataccg gcgcggtcgg caaggtaata caggcggtta
    10741 ccctcttgca ccagacgagc gccaaaacag ggcctcactg tgcagggaag cccccaccag
    10801 gggcggtcgt gattgtcatc gggatgagtt atcccggaga gtttgtctga cacgataaaa
    10861 tcctctctga aaagtggtta agaatggttt aacgatgaca ggtcagccgg cgcgctgacc
    10921 ggtttcggtg aagatgtatg cattgatgag aatggcctca tccacagcgg cgtctgaggt
    10981 catctggtcg tactcgtttc ccgggtgacg gaacatcagt tcagggtgac actcatcagc
    11041 cggacatacg ggccaaaact gtccttacgg cgttctgcaa acacggcaag aacgccggga
    11101 atatcctgca cttcacgacc agtatacgct tcagcactgc cgtgccagcg atacttaccg
    11161 gtacagaacg gaaaaagacg ggatgcagga tgctgttggt gaatacgcat ggcttcacca
    11221 cgggtgatga ttctcatgat gtgatacctc tgaagacaga agataaaagt gaaaacaggt
    11281 gtgatgtggt tgtgacgggt taaagcagac catgttcggc aaaggagaaa acctggttgc
    11341 caccgactat cagatggtcc ggcacccgga tatccaccag gcccagtgcc tgtaccagac
    11401 gttcggtgat aagccggtct gccttactgg gtgtgacttc accggacggg tgattgtgcg
    11461 ccagcaccac ggcagcggca ttgtggtaca gggcgcgttt aatcacttcc cggggatgaa
    11521 cttccgtacg gttgatggtg ccggtgaaga gggtttcacc ggcaatcagc tgattctggt
    11581 tgttcagata cagcacccgg aactcttccc gctccagtcc cgccatgttc agaatcagcc
    11641 attcccgtgc cgcacgggtg gaggtgaagg ccacgccggg ttcatgaaga tggcggtcca
    11701 gggtttgcag ggcccgcaga atgagactgc gctcgccggg cgtcatgttt ccgggcagaa
    11761 aggaaagctg ctgcattgtc cctgcctcca ttcagtcgat gatgcgcata atggcgctgc
    11821 attccggatg ctgcagggcg taatcccgca gccggtaata atggaccgtc atggcataac
    11881 actccgtacg acaggcatga tgactgtacg tcatcagaca ggcagcaatg ccggcggctt
    11941 ccgggctcat ttcagcgcgg ttaccgttca tggcattgaa cagtacccat gtttcgtcat
    12001 catcgtcatc cggttcgggt gccataaatg ccccgccgtt gttcagggtg tacagattcc
    12061 agataccacc gcagtagtct tcacacagac ggtccatcca gccgaagaca cggggttcca
    12121 gggtgaccca ctgtggaatg aggccaaaat gctgcggcca gaagctgatg cgctgttcat
    12181 cagggacggg agtggcaacc agttgttgca ggccagtctc cggtgcacag gccgaagcgg
    12241 tggtgttctg agacaatgtt ttcatgtatg aaatccttgt aatgtgaata aatgagaggg
    12301 agtaaaggtc gggagctggc aacatcacgt tcatcactga ccgtgatgac ccggtaatat
    12361 cagggatgaa tgatattcgg ctgaaattcc ggacagccgt acgcgtaacc gttccggtca
    12421 cagacggatg gccagatgcc attgcctgta tactcagtct gcacagactg atttctccgg
    12481 gacggtaaag cagacgggta caccaggcgc acaggtcatc atcagaaatg gaacgacacc
    12541 ccggcagcgc ctccggggcg tcgggtgatt ctggtgcctg aatcacgcct tcagggacat
    12601 gctgcctcag cgcaggctct ccagcagcgt ttctgccatt acccacaatg cgcggttgag
    12661 cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg atacgttttc cttttgcact
    12721 gcggccggaa atcccgcctt tcagcatatt ctcctggatg gtctgatatg cgctccacag
    12781 gtctttaccg taatcctccc tgcgtcgtgg tgtcagaatg tcagcggtgg tgactggctg
    12841 atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg
    12901 cggcaggacc agcgactgca tggcatcacg cttctcctct atccggtcaa acacgcccac
    12961 cacctcgtaa gccccttcaa taactttctc caccacattt ccccggtgtg gaacacgcaa
    13021 ttctcccaga gactgaccgc agaccagtcc attggtacaa atggcacgaa aatatccggg
    13081 taacatctgg tagctggatg aaccgtcatg agagttgagc agaataattt cagggacatg
    13141 ctgaccggtt atctgtccgg cccgccgcag acgcagcatg tgttttgtgt attcccggcg
    13201 acccgggtca cgcacacgtg tctggcaggc gaagaatggc tgaaagcctt cccgctgcag
    13261 gctttccagt acggtgatgg tgggagtgta cgcatagcgt ttactgcggg aagtatgccg
    13321 gtcttcaccg aaaatactgg gaacatagtg catcagttct tcgtgtgtca gcggacggtc
    13381 acggcgtatc tggtttgcag cataaccaaa acgactggct agtcgcataa tttgctcctt
    13441 atcggtggtt aagatttact ggtgtaataa atgaaaaagc cacgtctccc ggagaagacg
    13501 cggcctgatg gacgaaatta atgaggttta ttgtctgaga agaccgtcac tggcgaacct
    13561 tccggcgtca gtctctgcgt tgcggataac ccgacggaaa tgagttccct ggtcatcgtc
    13621 gataaacaca ttgcggtaag ctgttgtgat gacaacagcc tgctcgcgtg agaaagggac
    13681 ttcgggcaat aaacgtgaca tacaaccaga cctgtatttg gttccctgaa aatataagtc
    13741 cctgtcatca tggcagagcc gtatttactt ttgatatcaa taaattaata acgcgaagat
    13801 gaaccgcata acttacgcct ttaatttgtt ttgtttacgt agtccctgac caggtacgcg
    13861 aattcgtgaa cgccagtttc accaaaccaa atcaggtaac cagggtcgat gttgtaatag
    13921 taaccaacga ctttatgcgt ttttgggtta tagagtgtta cgatatagtc ccagccgcag
    13981 gcgtgggctt tacaaccgcc accttctacc caggtcgttc cgttaatgtt tacaggatac
    14041 gcaccaccat caggaccatc catatagccg tcttccccaa acattccgtt taacccaaca
    14101 gggtttaatg ctttgtcgat agcagctctg taggtagcgt caccttcgta taactcgtac
    14161 gttgttggac atgcaccagg ctggtcagcc cgggcttttg agtcgcattt aagtgtctga
    14221 tttacagtgg cggcgtttgt gtcaggact
//
LOCUS       DAJVRP010000101        13964 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVRP010000101 DAJVRP010000000
VERSION     DAJVRP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.101"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OQN12_003238"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OQN12_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1655464.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OQN12_003239"
     CDS             2219..2767
                     /locus_tag="OQN12_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1655465.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OQN12_003240"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OQN12_003240"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1655466.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OQN12_003241"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OQN12_003241"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1655467.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OQN12_003242"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OQN12_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1655468.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OQN12_003243"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OQN12_003243"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1655469.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OQN12_003244"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OQN12_003244"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1655470.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="OQN12_003245"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="OQN12_003245"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1655471.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OQN12_003246"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OQN12_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1655472.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OQN12_003247"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="OQN12_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCS1655473.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3552 a         3562 c         3525 g         3325 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgtcg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DAJVRP010000102        13956 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVRP010000102 DAJVRP010000000
VERSION     DAJVRP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.102"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="OQN12_003248"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="OQN12_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1655474.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="OQN12_003249"
     CDS             complement(1604..1777)
                     /locus_tag="OQN12_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1655475.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="OQN12_003250"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="OQN12_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1655476.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="OQN12_003251"
     CDS             3415..3744
                     /locus_tag="OQN12_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1655477.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="OQN12_003252"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="OQN12_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1655478.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="OQN12_003253"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="OQN12_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1655479.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="OQN12_003254"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="OQN12_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1655480.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="OQN12_003255"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="OQN12_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1655481.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="OQN12_003256"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="OQN12_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1655482.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="OQN12_003257"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="OQN12_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1655483.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="OQN12_003258"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="OQN12_003258"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS1655484.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3661 a         3454 c         3244 g         3597 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcgc tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaaagac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacagctgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag cggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cttggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgcttg agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccaag tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggtta accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgagcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga caacgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttaccccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtataacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttttcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaagct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaaacagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcaga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DAJVRP010000103        13797 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVRP010000103 DAJVRP010000000
VERSION     DAJVRP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.103"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OQN12_003259"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OQN12_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS1655485.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OQN12_003260"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OQN12_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS1655486.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OQN12_003261"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OQN12_003261"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS1655487.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OQN12_003262"
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OQN12_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCS1655488.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OQN12_003263"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OQN12_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS1655489.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OQN12_003264"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OQN12_003264"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS1655490.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OQN12_003265"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OQN12_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS1655491.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="OQN12_003266"
     CDS             7489..7638
                     /locus_tag="OQN12_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655492.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="OQN12_003267"
     CDS             7839..9422
                     /locus_tag="OQN12_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1655493.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OQN12_003268"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OQN12_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1655494.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OQN12_003269"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OQN12_003269"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1655495.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OQN12_003270"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OQN12_003270"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1655496.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..>13797
                     /locus_tag="OQN12_003271"
     CDS             13381..>13797
                     /locus_tag="OQN12_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCS1655497.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3192 a         3640 c         3494 g         3471 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagtatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctggcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgagtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgcgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa
    13681 gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat
    13741 ctggctgact ggctgcgtct ggcattcggc agcgccattg ttccgcctct gttgggg
//
LOCUS       DAJVRP010000104        13525 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVRP010000104 DAJVRP010000000
VERSION     DAJVRP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.104"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..830)
                     /locus_tag="OQN12_003272"
     CDS             complement(<1..830)
                     /locus_tag="OQN12_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCS1655498.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            complement(1036..1755)
                     /locus_tag="OQN12_003273"
                     /pseudo
     CDS             complement(1036..1755)
                     /locus_tag="OQN12_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1771..2103)
                     /gene="cusF"
                     /locus_tag="OQN12_003274"
     CDS             complement(1771..2103)
                     /gene="cusF"
                     /locus_tag="OQN12_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCS1655499.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(2127..3509)
                     /gene="cusC"
                     /locus_tag="OQN12_003275"
                     /pseudo
     CDS             complement(2127..3509)
                     /gene="cusC"
                     /locus_tag="OQN12_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            3666..4349
                     /gene="cusR"
                     /locus_tag="OQN12_003276"
     CDS             3666..4349
                     /gene="cusR"
                     /locus_tag="OQN12_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCS1655500.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            4339..5781
                     /gene="cusS"
                     /locus_tag="OQN12_003277"
     CDS             4339..5781
                     /gene="cusS"
                     /locus_tag="OQN12_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCS1655501.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5931..8168
                     /gene="nrfB"
                     /locus_tag="OQN12_003278"
                     /pseudo
     CDS             5931..8168
                     /gene="nrfB"
                     /locus_tag="OQN12_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            8155..11123
                     /gene="nfrA"
                     /locus_tag="OQN12_003279"
                     /pseudo
     CDS             8155..11123
                     /gene="nfrA"
                     /locus_tag="OQN12_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            11124..12014
                     /locus_tag="OQN12_003280"
     CDS             11124..12014
                     /locus_tag="OQN12_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCS1655502.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            12197..12958
                     /gene="envY"
                     /locus_tag="OQN12_003281"
     CDS             12197..12958
                     /gene="envY"
                     /locus_tag="OQN12_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCS1655503.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(13004..13080)
                     /locus_tag="OQN12_003282"
     tRNA            complement(13004..13080)
                     /locus_tag="OQN12_003282"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13044..13046),aa:Arg,seq:tct)
     gene            13323..>13525
                     /locus_tag="OQN12_003283"
     CDS             13323..>13525
                     /locus_tag="OQN12_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCS1655504.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3300 a         3508 c         3426 g         3291 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccaacgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgt
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataagc aacctacctt cctctttcgc gaccacgtta tctttggcga tattcattcc
     1081 cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg taaagcgagt
     1141 ctggattttt tgcgtggcga tcaggtggcg aatatccgcc tccggcattc ccgccagtcg
     1201 cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcaacagtaa
     1261 atactcactc tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg gtgtgccctt
     1321 ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg cagcgcgagc
     1381 ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa
     1441 agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg
     1501 gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat
     1561 atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca tcgggtcgta
     1621 ccagaataag attttacgtt ctgcggtcgg cattttttct gtgtgcggtt ccgcctggtc
     1681 aaaccaggta aaacctgccg ctgaaataat accgcccgcg atcatgctgc cgataataag
     1741 cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat cctgtaataa
     1801 agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt taatttcact
     1861 cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt tgacggcagc
     1921 aatcggatcg tgatggatgg tgattttttt gctttccaga tcaacacctt ttaccacacc
     1981 ggtggcacta atgacctgtg gttgtgcttc gctcatggtt tcatgatgat gttcgttagc
     2041 ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca gtgctttttt
     2101 catttattct ctcctggagt taaaaattat tgctgccaac cgccccctaa ggcggtatac
     2161 aaataaattt cgttaacctg acgggcataa ttcaggtcaa gtaaagtttg tcgggttgca
     2221 aataaagaac gctcggcatc cagcacttcc agataactta ccgcgccgtg ctgatataat
     2281 gcccgcgccc gttgcagggt aatttgcagc gacgccagat aacgctgctg ggcgctgatt
     2341 tgatcgtcca ggctttgacg taatgccagc gcatctgcca cctctttaaa ggcgttctgg
     2401 attttctgtt cataattcac caccgactgc tgctggcgaa tttcggcgat atccagattg
     2461 gcctggttgc gtccggcatt aaaaatgggg atctcaattt taggaataaa attccacatc
     2521 ccgctgctag cgttaaataa tgacgacaga tcgctgctgg cggttgatat tccgctggtc
     2581 aggctgatag acgggaaaaa tgccgcacgc gcagcaccaa tattagcatt agccgccatt
     2641 aacgcgtgtt cagcttccat aatatccggg cgctgcaata agatttgcga cgacaagccc
     2701 gccggtaatt taacgctttg caggctgccg ctgtttactg tctgcgcttg cggtagcttg
     2761 ccgtagcttc ccaataacag ttgcaatgca ttattcgcct gcgccagttc cccctgacgt
     2821 ttagcgatgt cactgcgggt actttctatc accccgcgag cctgttccag cgccagaaca
     2881 ttgctgctac cggtcaacag ttgtttttcg acaaacgcat atgactgctg ataattacgc
     2941 agcgtttctt cggctatttg caattgcgca tacgccagct gctgattgaa atagctttgc
     3001 gcgacattag aaaccagcaa aatatgcacc gcgcgctggg cttcctcagt ggctaaataa
     3061 ttttggcgct cggcttcgct catgttcttt aagcgaccga aaaaatcgag atcaaagctg
     3121 gcgttaaggc cagtcgagaa ctcccgcgtc gtggctgaat cgcctttaag attgccgctc
     3181 cagctgccgc tgccctagcc attgagctgt gggtagcggt cggcatcggt cagacgatat
     3241 tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccggtt attcaccagc
     3301 gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca gcccacgttc
     3361 tggtagttat ccgctgcgtt aaccaggccg ttctggctga gtgagaactg ctgcggtacg
     3421 ggcattaccg gacgctgata atccggtgcc agtgaacaac cagttagcgc aagggccaca
     3481 caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat tttatgtcgc
     3541 caactttact cgccaggctc tgattttccg gtgacaggaa aatgacaaaa ttgtcatttt
     3601 gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca acatgcggag
     3661 gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac ttgaccaaag
     3721 ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggctgaat ggctaccatc
     3781 tggcgatgac cggtgattat gacctgataa tcctcgatat tatgctgcca gacgtgaacg
     3841 gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg gatgccgatt ctgttgctta
     3901 ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggca gatgactacc
     3961 tggtgaaacc gttcgctttt gctgaactgc tggcgcgggt gcgcactctc ctgcggcgcg
     4021 gggcggcggt gattatcgaa agtcagtttc aggttgccga tctgatggtc gatctcgtca
     4081 gccgcaaagt caccctcagc ggcacgcgca tcactctgac cagtaaagaa tttactctgc
     4141 tggagttttt tctccgccat cagggcgaag tgctgccccg ctcgcttatc gcctcgcagg
     4201 tatgggacat gaatttcgac agcgacacta acgccattga tgtggcggtg aagcggctac
     4261 gcggcaaaat cgacaacgac tttgagccga agctgattca aaccgtgcgc ggcgtgggtt
     4321 acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc gctggcaacc
     4381 cgcctgacct tttttatcag cctggccacc atcgcggcgt ttttcgcctt tgcatggatc
     4441 ataatccact cagtaaaagt gcattttgcc gagcaggaca ttaatgattt aaaagagatt
     4501 agcgccaccc ttgaacgggt actaaatcac cctgacgaaa cgcaagcccg acgcttaatg
     4561 acgctggaag atatcgtcag tggttattcc aacgtgttga tttccctggc agatagccac
     4621 ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc gcgagtttgc acgtgatgcc
     4681 atacccgata aagacgcgcg gggtggcgag gtgtatctcc tttccggccc gacgattatg
     4741 atgccaggcc acggtcacgg gcatatggaa cacagcaact ggcggatgat taacttgccg
     4801 gttggcccgt tggtggacgg caaaccgatt tatacgctct acatcgcgct gtcgatcgat
     4861 tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc atcggtaatc
     4921 agcatcctga tcgtctttat cgtgctgttg gcggtacata aaggtcacgc gccgatccgc
     4981 agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc tcgacgttcg ccttgacccg
     5041 cagaccgtgc cgattgagct ggaacagctg gtactgtcgt tcaaccatat gatcgagcgt
     5101 attgaggatg tctttactcg ccagtccaat ttctcagcgg atatcgccca cgaaattcgc
     5161 acaccaatta ctaatctcat tacgcaaacg gaaatcgccc tcagccagtc gcgcagccag
     5221 aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat ggcgaaaatg
     5281 gtcagcgata tgctgtttct cgctcaggcc gataacaacc agctaatccc cgaaaagaaa
     5341 atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc gctagcggaa
     5401 gatcgcggcg tggagttgcg gtttgttggc gacaaacgtc aggttgcggg cgatccgttg
     5461 atgctgcgtc gggcgctaag caacctgctt tctaatgccc ttcgttatac gccaaccaga
     5521 gagacaattg tagtacgctg tcagacggtc gatcaccagg tgcaagtttc cgtcgaaaac
     5581 cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt ctatcgcgtt
     5641 gacccctccc gccagcgaaa aggtgaaggt agcggtattg ggctggcgat agtgaaatcg
     5701 attgttgtcg cgcataaagg cacggttgcg gtaacgtcag atgcgcgggg gacaaggttt
     5761 gttatcacat tacccgctta attattcagc aaacggcaac ttttataacc agtgtaaaaa
     5821 taacgtgccg caatagtcat acatattaat ggtaaaaagc tgtcacaatt cataaaaaac
     5881 cttaatatac gccaccctaa acataaccag cgttaatgta aggtttttgt gtggactggc
     5941 ttcttgatgt ttttgctacc tggctctacg gcttaaaagt aatcgcgata acgttagcgg
     6001 tcatcatgtt catcagcggg ctggacgatt tttttattga tgtcgtctac tgggtacgcc
     6061 gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac cgcgaactgt
     6121 ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa acgggcgtca
     6181 tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat catatctttg
     6241 ttggcaccta ccccaacgac cccgatactc aacgtgatgt tgacgaagtg tgcgctcgct
     6301 ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc caccagcaaa gccgactgcc
     6361 tgaacaacgt gctggacgcc atcacccagt ttgaacgtag cgccaatttc gcttttgctg
     6421 gttttattct gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt ctgttcaact
     6481 atctggtcga gcgtaaagat ctgattcaga tcccagtgta tccgtttgaa cgcgaatgga
     6541 cgcacttcac cagcatgact tacattgatg agttttcaga actgcatggc aaagatgttc
     6601 cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc tgtttcagcc
     6661 gccgcgccgt gaccgcactg ttagctgacg gtaacggtat tgctttcgac gtgcagagtc
     6721 ttactgaaga ttacgacatt ggcttccgcc tgaaagaaaa aggtatgacg gaaatttttg
     6781 tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgtaaattt ttacagcacg
     6841 cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc cgataccttt tcgactgcgg
     6901 ttcgacaaaa atctcgctgg atcatcggca ttgttttcca gggctttaaa acccataaat
     6961 ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg gcaatcagta
     7021 actttgtcag cttcctcgcg atgctggtga tgatccagct tttgctgttg ctggcgtatg
     7081 aaagtttgtg gcccgatgcc tagcatttcc tttctatttt tagcggcagc gcatggttaa
     7141 tgaccctgct gtggctaaac tttggcttga tggttaaccg catcgtgcag cgagtgattt
     7201 tcgtcactgg ctactacggc ctcacgcagg gcctgctttc cgtcctgcgt cttttctggg
     7261 gcaacctgat taactttatg gctaactggc gcgtgctgaa acaggttctt cagcacggcg
     7321 atccgcgtcg cgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtc actggcgata
     7381 cccgctcgtt gcgcccgtta ggtcaaattc tgctggaaaa tcaggtcatc actgaagaac
     7441 aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt tcaatgctga
     7501 tgcaggggct gattagcgcc gagcagctgg cacaggcgct ggcagagcaa aacggcgtgg
     7561 cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct gattgccgaa atgccagcct
     7621 ccgtagcgct gcattatgca gtactgccgc tgcgtctgga aaatgatgag ttaattgtcg
     7681 gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa gtcggacgca
     7741 aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac agggttacgc cactggtatg
     7801 cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag catcagtggc
     7861 tcacggaaca gcagaccggt gaaatctggc ggcaatatgt gccgcatcag ttcctgttcg
     7921 ccgaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg ttgttattgc
     7981 gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc gttatcagcc
     8041 aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg atgcaatcac
     8101 tattactcaa agcaggttta aacacagaac aggttgcgca actggagtcc gaaaatgaag
     8161 gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacgtc tttattgagt
     8221 accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct gagcgattat
     8281 cgccattttg ttatttatcc ccgtctcgat aaggcgctga aggcacagaa aaataacgac
     8341 gaagcaaccg ccatccgcga atttgaatat atacaccaac aggtgccgga taatattccg
     8401 ctgactttat accttgcgga agcctatcgt cattttggtc atgatgaccg ggcgcggctg
     8461 ttgcttgagg atcaactgaa acgtcaccca ggagatgccc gacttgagcg cagtctggcg
     8521 gctattccag ttgaagtgaa aagcgttacg actgttgaag aactgcttgc ccagcaaaaa
     8581 gcgggcgacg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa taccctgcgg
     8641 ctggcacaat tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc tgcatcgccg
     8701 gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa tctacctgaa acaatgatcc
     8761 caggcagata cgctatacaa tgaagcacgc cagcagaaca cattaagcgc ggcagaacgc
     8821 cgtcagtggt ttgacgtgct tcttgccggg cagctggacg atcggatcct ggcactgcaa
     8881 tcacagggga tcttcactga tcctcagtca tatattactt acgcgaccgc gctggcttat
     8941 cgtggcgaaa aagcacgcct ccagcattat ctcattgaaa ataagccgct gtttaccacg
     9001 ggcgcacaag agaaaagttg gctctatctg ttatctaaat acagcgccaa ccccgttcag
     9061 gcattggcga attatacggt gcagtttgcc gataaccgcc agtatgttgt tggcgcgacg
     9121 ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct cgccaccctc
     9181 cccgccaatg aaatgcttga ggagcgttat gctgtcagcg tggcgacccg taacaaggct
     9241 gaatctctgc gtctggcacg attgctgtat cagcaagaac cgacaaatct tccccgcctg
     9301 gatcaactga cctggcaact gatgcagaac gagcagtcac gcgaagctgc cgatttattg
     9361 ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc agactttaat ggcgcgactg
     9421 gcgtctctgc tggaaagtca tccttaccta gcaacgccgg cgaaggtggc gattttatcg
     9481 aaacccttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg tattgcagat
     9541 aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcctacga tgctgccgcc
     9601 tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgtt gtatgcatgg
     9661 cttcaggccg aacaacgaca accgagcgcc tggcaacatc gtgcggtagc ctatcaggcg
     9721 tatcaggttg aggactacgc caccgcactg gcggcctggc agaaaatcag tcttcacgac
     9781 atgagtaatg atgatctgct cgccgccgcc aacaccgctc aggcagcggg aaatggcgcg
     9841 gctcgcgatc gctggcttca acaggcagaa caacgtgagc tgggaaacaa tgctcaatac
     9901 tggtggctgc atgcgcgacg ttatatccca gctcagccag aactcgcact gaacgatctc
     9961 acgcgctcaa tcaatattgc cccttctgcc aactcttacg ttgcgcgggc gacaatttat
    10021 cgccaacgtc ataatgttcc ggccgcggtg agtgatttgc gcgccgcgct ggaactggaa
    10081 ccaaataata gcaacaccca ggcagcgctt ggttacgcct tgtgggatag cggtgatatc
    10141 gcacagtcgc gggaaatgct cgaacaggcg cataaagggc ttccggacga tccggcactg
    10201 atccgacaac tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac gcagcactac
    10261 gcccggctcg tgattgatga cattgataat caggcataac cccactgacc ccagagcaaa
    10321 atcagcaacg cttcaatttc cgccgtttgc atgaggaggt cggtcgccgc tggacgttca
    10381 gtttcgattc ttccatcggc ttgcgttccg gcgcaatgag taccgctaac aataatgtcg
    10441 gcggtgcagc gcctgggaaa agctatcgta gctacggaca actggaagcc gagtaccgca
    10501 tcggacgcaa tatgctgctg gaaggcgacc tgctctcagt ttatagccgc gtctttgccg
    10561 ataccggaga aaacggggtg atgatgccgg tgaaaaatcc gatgtccggc accggtctgc
    10621 gctggaagcc gctgcgcgat cagatctttt tcctcgccgt cgaacagcag ttgccgctga
    10681 acgggcaaaa tggcgcatcc gataccatgc tgcgcgccag cgcctcattc tttaatggcg
    10741 gcaaatacag cgacgaatgg cacccgaacg gttcaggctg gtttgcccaa aacctgtacc
    10801 tcgatgcggc gcaatatatc cgccaggata ttcaggcgtg gacggcagat tatcgcgtca
    10861 gctggcatca gaaggtagct aacggacaga ctattgagcc ttacgctcac gttcaggaca
    10921 acggctatcg tgataaaggc actcagggcg cgcagcttgg cggtgtcggg gtccgctgga
    10981 atatttggac cggcgagacg cactacgacg cctggccgca caaagtcagt ctcggcgtcg
    11041 agtatcaaca tacctttaag gcgattaatc aacgtaacgg agagcgcaac aacgcgtttc
    11101 tcaccattgg agtgcactgg taaatgcgta agttcatttt cgtattgctg acactgcttt
    11161 tggtcagccc tttttccttt gcgatgaaag gtattatctg gcaaccacaa aaccgagata
    11221 gtcaggttac cgatacccag tggcaggggc tgatgagtca gttacgtttt caaggcttcg
    11281 ataccctggt tttgcaatgg acccgttacg gcgatgcatt tacccagcca gaacagcgca
    11341 cgttattgtt taagcgtgcc gcagctgcgc aacagactgg cctgaagctg attgtcgggc
    11401 tgaacgccga tccggaattt tttatgcacc agaaacagtc gtctgcagcg ctggaaagct
    11461 atcttaatcg cctgctggct gccgatctcc agcaagccag attatggagc gccgcgcctg
    11521 gcataacgcc ggatggctgg tacatcagcg cggaaattga cgacctgaac tggcgcagcg
    11581 aagccgcccg tcaacctttg ctaacatggt taaacaacgc gcagcggctg attagcgatg
    11641 tttcagcaaa accggtttat atcagtagtt ttttcgccgg aaacatgtcg cccgatggct
    11701 atcgccaact gctggaacaa gttaaagcaa ccggagttaa tgtctgggta caggatggca
    11761 gcggcgtgga taaacttacc gctgaacagc gtgaacgtta tctacaggcc agcgccgatt
    11821 gccaaagctc cgccccagca agcggcattg tttatgaact gtttgtcgcc ggcaaaggca
    11881 aaacctttac agcgaaaccg aaaccggacg cagaaattgc ctcgctgtta gcgaaacgtt
    11941 cctcttgcgg taaagacatg ctctatttct ctctgcgcta tttgcccgtc gcgcacggca
    12001 ttctcgagta ttaaatcccc tccaggtaag tcgggtacga cctggcttac ctctttcgct
    12061 ctcgtcagaa ttccatcaag attaattgct aaaaagcatg ctaaagaaag aagatcgtta
    12121 tcggcgaacg ggccgatcac tcatgcagga agcatagatc ctttgagcgg agtttctgat
    12181 atctgaggag tgcgaaatgc aattgagcag cagtgaacct tgcgtggtga tcctgaccga
    12241 aaaagaggta gaggtaagcg tcaataacca tgctacgttt acccttccga aaaactacct
    12301 ggccgccttc gcgtgcaaca ataacgtcat tgaactctca acgttaaatc acgtattaat
    12361 cacccacatc aaccgtaaca tcatcaacga ttatctgttg tttttaaata agaaattaac
    12421 ctgtgtaaag ccctggtcgc ggctggcaac cccggttatc gcttgtcata gccgtacacc
    12481 ggaagtgttc cggctagcca ccaaccacag caagcagcaa tccagcaaac cctgcgaagc
    12541 ggagttgacg cgcgcattac tttttaccgt attgtctaac tttcttgagc aatcgcggtt
    12601 tattgcccta ctgatgtata tcttacgcag cagcgtccgc gacagcgtct gccgcattat
    12661 tcaaagcgat attcagcatt actggaatct gcgaattgtc gccagttcgc tatgtttaag
    12721 ccccagcctg ctcaaaaaga aattaaaaaa cgaaaatacc agctatagcc agattgtcac
    12781 agagtgtcgt atgcgttacg ccgtacagat gttattgatg gataacaaaa atatcactca
    12841 ggtggcgcaa ttatgtggct atagcagcac gtcgtacttt atctctgttt ttaaggcgtt
    12901 ttacagcctg acaccgttga attatctcgc caaacagcga gaaaaagtga tgtggtgaag
    12961 ggcaaagcgg aaacggataa gacgggcata aatgaagaag aaatggcgcg ccctgcagga
    13021 ttcgaacctg cggcccacga cttagaaggt cgttgctcta tccaactgag ctaagggcgc
    13081 gttgataccg caatgcggtg taatcgcgtg aattatacgg tcaacccttg ctgagtcaat
    13141 ggcttttgat ctggttgctg aacaagtgaa cgaccgcgtc tgattttctg atttatttcg
    13201 ctatagcggc aaacaaacgc acaccgctgc gcgtctgaat caagaaaacc cgtattttca
    13261 tgtatcaaag tacaatttac cgccctaacg gaaaattgtc cgctcctatg agactggtaa
    13321 ctatgaaacc aacgtcggtg atcattatgg atactcatcc tatcatcaga atgtctattg
    13381 aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat gattaccgca
    13441 taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat atagacttgc
    13501 ccggaacaga cggttttacc ttcct
//
LOCUS       DAJVRP010000105        13186 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVRP010000105 DAJVRP010000000
VERSION     DAJVRP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.105"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..605)
                     /locus_tag="OQN12_003284"
     CDS             complement(<1..605)
                     /locus_tag="OQN12_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1655505.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNT"
     gene            846..1646
                     /locus_tag="OQN12_003285"
     CDS             846..1646
                     /locus_tag="OQN12_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1655506.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1724..2494
                     /locus_tag="OQN12_003286"
     CDS             1724..2494
                     /locus_tag="OQN12_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1655507.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2542..3900)
                     /gene="mltD"
                     /locus_tag="OQN12_003287"
     CDS             complement(2542..3900)
                     /gene="mltD"
                     /locus_tag="OQN12_003287"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1655508.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(3972..4727)
                     /gene="gloB"
                     /locus_tag="OQN12_003288"
     CDS             complement(3972..4727)
                     /gene="gloB"
                     /locus_tag="OQN12_003288"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1655509.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            4761..5483
                     /locus_tag="OQN12_003289"
     CDS             4761..5483
                     /locus_tag="OQN12_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1655510.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5480..5947)
                     /gene="rnhA"
                     /locus_tag="OQN12_003290"
     CDS             complement(5480..5947)
                     /gene="rnhA"
                     /locus_tag="OQN12_003290"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1655511.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6012..6743
                     /gene="dnaQ"
                     /locus_tag="OQN12_003291"
     CDS             6012..6743
                     /gene="dnaQ"
                     /locus_tag="OQN12_003291"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1655512.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            6876..6952
                     /locus_tag="OQN12_003292"
     tRNA            6876..6952
                     /locus_tag="OQN12_003292"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6910..6912,aa:Asp,seq:gtc)
     gene            complement(7083..8129)
                     /locus_tag="OQN12_003293"
     CDS             complement(7083..8129)
                     /locus_tag="OQN12_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCS1655513.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8140..9125)
                     /gene="tssG"
                     /locus_tag="OQN12_003294"
                     /pseudo
     CDS             complement(8140..9125)
                     /gene="tssG"
                     /locus_tag="OQN12_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9122..11005)
                     /gene="tssF"
                     /locus_tag="OQN12_003295"
     CDS             complement(9122..11005)
                     /gene="tssF"
                     /locus_tag="OQN12_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCS1655514.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11021..11515)
                     /gene="tssE"
                     /locus_tag="OQN12_003296"
     CDS             complement(11021..11515)
                     /gene="tssE"
                     /locus_tag="OQN12_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCS1655515.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11512..12342)
                     /locus_tag="OQN12_003297"
                     /pseudo
     CDS             complement(11512..12342)
                     /locus_tag="OQN12_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12329..13180)
                     /gene="tagK"
                     /locus_tag="OQN12_003298"
                     /pseudo
     CDS             complement(12329..13180)
                     /gene="tagK"
                     /locus_tag="OQN12_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596542.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3021 a         3428 c         3538 g         3199 t
ORIGIN      
        1 caggtattga gggaagcggg ttcagtgaat cccaggcaaa catgttgctt taaatcgtcg
       61 atcgtttctg gcttcccgta gcgggaaata taatcggggg aggcgataat ttttcgataa
      121 ctgttaaata acggcctggc acgtaagctg gagtccgtta acgtaccagc gcgtatcgcg
      181 acatccactt ttctttcgat caaattaata atcgtttcgg aggagactag cgataaagtg
      241 acttccggat agcgttcacg gaaaggctta attaacggca tcagaaagtg cagcaccact
      301 ggagttgcgg catcgatccg taacagtcca cgcggtgtat tacgcgtctc cataatttct
      361 gattctgccg ctgccatctc ctgcaaaatt gactgtacgc gacgaaaata acgctcgcct
      421 tcttccgtca ggctaagttg ccgcgtggtc cgattaagca ggctaacgcc aagtttcatt
      481 tccagctttt tcaccgcccg gcttaccgct gagtttgctt gccctaattg ttccgctgcc
      541 cggctaaagc tgccgctttc gacgaccgaa acaaaaatgg cgagttcttc cgacgtggct
      601 ttcatttttg ctcctgttgc aaaatagatg agatattttg aatttatttg tcattaaacc
      661 atcaggatgc gtgatatgtc atccgattta atgttctcaa taatgagcaa atttctgaca
      721 ggtgtaagca cttgcttaca taacaatata caattgctcg ttgaaagagt gagctaaaat
      781 ccctataaca gtagaaccct cccgagtgcg gaagggttga cgtaatagag gtttcaaagt
      841 caaaagtgcg aaaaaacacc tatgccatgc gctatgttgc cggacaacct gcggaaagga
      901 tcttaccgcc ggggtctttt gcgagcatcg gccaggcatt accacctgga gaaccgttaa
      961 gtaccgaaga gcgtattcgg atcctggtgt ggaacatata caaacagcaa cgcgctgaat
     1021 ggttgtcggt attaaagaac tacggcaaag atgcacatct ggtgttattg caggaagcgc
     1081 agacaacgcc agagttagta cagtttgcga ccgctaacta tcttgccgcc gatcaggtgc
     1141 ccgctttcgt gctgccacaa catccttccg gtgtaatgac cctttcggcg gcgcatccag
     1201 tgtattgctg cccgttacgt gaacgagaac ccattttgcg tctggcgaag tcggcactgg
     1261 tgacggtcta tccattacct gacacccgcc tgttgatggt ggttaatata cacgccgtca
     1321 acttcagtct gggcgtggat gtctatagta agcagttact tcctattggt gatcagatag
     1381 ctcaccacag cggcccggtc attatggcgg gagatttcaa tgcctggagc cgtagaagga
     1441 tgaacgcgtt atatcgcttt gcgcgggaaa tgtcgctgcg ccaggtgcgt tttaccgatg
     1501 atcagcgccg tcgggcgttt ggccgcccgc tcgattttgt tttctaccgt ggtctgaacg
     1561 tcagcgaagc ttctgtattg gttacgcgcg cttccgatca caatccgcta ctcgttgaat
     1621 tcagtcccgg caagcctgat aaataaggta tgtcaggtct gccacagggc agaccaacgt
     1681 ttggcgctgt ccttttatac tcaaacaaca aaaggacagc gcaatgacaa cacaatccca
     1741 ccatgaccac gtagaaaagc agtttagctc tcaagcctgc gaatatctga ccagtaccgt
     1801 acatgcatcc ggacgagatt tacagcgcct ggcggtgcgt ctggctgatt atcctggtgc
     1861 aagtgcactt gatatgggct gcggagcagg gcatgccagc tttgttgctg cgcaaaacgt
     1921 gagcgcggtg gtggcgtatg acttatctgc ccaaatgctg gatgtcgtgg cacaagctgc
     1981 cgaagcccgg caactgaaaa atatcaccac ccgccaggga tatgccgaaa gtctgccatt
     2041 tgccgataac gcatttgata ttgttatcag ccgttattct gcccatcact ggcatgatgt
     2101 tggtgcagca ctgcgtgaag tgaaccggat attgaaacct ggcggtagga tgattgtgat
     2161 ggacgtaatg tctccgggtc atccagtgcg cgacatctgg ttacagacgg tagaagcatt
     2221 acgcgatacc tctcacgtac gaaactacgc cagcggtgag tggttgaggt taatcaatga
     2281 agccaatctg atagttgata atttaattac agataagtta ccgctggaat tttgttcatg
     2341 ggtcgcgaga atgcgtacgc cagaagcgtt agtagacgct attcgcattt accaacagag
     2401 cgcatcgaca gaggtgagaa cgtattttgc cttgcagaat gacggctctt tcaccagtga
     2461 tatcatcatg gtagaagcac ataaagcggc ataaataaaa aaggcaccgg gggaatcggt
     2521 acctttttat tatctggttt gtcaggaatc tggcatgttg ttgtttttca caaacaacgt
     2581 cagcttgtcg cctggttgca gattcgcagt gtcgctgttc cagcgcatca catctttgat
     2641 gttcacgccg tggcgtttag caatgcttga aagcgaatcg cctttgcgca cacgatacgt
     2701 aatgctatcg ctgttgtttg ccagtcgctg tgcgctactg cctgcaccaa tcgtcaaact
     2761 ttggcctggc ttcagcttag atccgcgcag tttgttccac tgctgcaaat ctttggtgct
     2821 tacgccgaga cgtgaagcga tgcttgaaag cgtgtcgcca gagcgtacgg tgtaaacacg
     2881 gctgttaagc ggcgtattgt tggcaaccag cgtcgactgt acagcagcaa tttcgcctga
     2941 agcgagagat tcacgcagtt gatctgcatg cttctttggc accatcacgt actgcggccc
     3001 acttgcgccc agcgtggagc ctttcacgcc agcgttgaat gtcttcagct tgctgacgga
     3061 aatccccgcc atatctgcaa ccttcgccat ttcaaccggg ctactcaggt gcacacgcgc
     3121 cagagcacgg ctttcatcgg tcgttggcag acgtacgcca taacgcttgc tgtttttgag
     3181 aatatcactc aatgccagca ttttaggcac gtactgcttc gtttcctgcg gcaacggtaa
     3241 cgaccagaag tccgtggatt tcccacgcgc tttgttcgtt ttaattgcct tcatgacccg
     3301 accttcgccg ctgttataag ccgctacggt cagaagccag tcgtcgtcaa acattttgtt
     3361 cagacgctgc atcatattca gcgcggcagt tgttgaagca acaacatcgc gacgcgcgtc
     3421 ataattgcgg gtctgtttca aaccataatt gcgccccgtg ctcggaatga tctgccagat
     3481 gcctgcggca ttggcgccag acgttgcgtg aggatcaaaa gcgctctcca ctatgggtag
     3541 tagtaccagt tccataggca tgttacgttt tttaacttgc cctgctatcc agtacatata
     3601 cggctctgcc cgtaaagtta catcgtggag atagctctta ttgcgtaaat atttctgttt
     3661 ctgttcgcga atccggtcat tttccggaat tcccatcttt agctcgtcgc caatgaaagc
     3721 ccacaagtca ccatctggcg cgatagacgt cccatcgtcc atccatcgtg cctgacttgt
     3781 aaactttgct gcttcccctt gaccagctgc agaaaggctc tgtgcgtgct gttgaacgtt
     3841 gccggtactc tggcaaccca cgagcaggac agaggcgagt aatatcgctt ttgccttcat
     3901 gtgtgtgtca atagttgctt aaaagacgac cgatcataac ggcgaacgga gccgatgaca
     3961 agaaagtttt atcagaacct atctttcttt gaccttaacc atgcaaaacg ctcttcaggt
     4021 tgttgcaata atgtttcttc attaattcca ttaattaaat caatatcttc cgttcttaaa
     4081 aaaacattaa tttgccgctc atttttcaga attacgggta gtgttatttg attttttgcc
     4141 cgtaactcct taactttacg ataataatca tttatggaca aatcgtgcgg aaaaatactc
     4201 aaagcaaact tcatatttga taacgtatat tcatgagcac aacataccaa tgtatcgtca
     4261 ggtaacgcac ttagcttttt aagtgattga tacatttgta aagctgtccc ttcaaacaac
     4321 cgaccacacc caccagaaaa cagcgtgtcg ccgcaaaata gataaggttt actgaagtaa
     4381 cagatatgtc ctaaagtgtg acccggtgtg gtaattacac taaattcatg ccctaaaacg
     4441 aaggcagttt cgccatcttt gactacctgt gttgttccct tatcttgtgt ctcttgtgga
     4501 ccatacacca caatttgcgg aaacttttcc accagttctt ttacgccgcc aacgtgatcg
     4561 tggtgatggt gggttagaaa tatggcctcc ggttgccagt tattggcggt aatggcgttt
     4621 aatactggct ctgcgtctcc gggatcgaca atcaggcagc gacctgcctc atcattcaaa
     4681 acccagatgt aattgtcatc aaaggcggga atactgttaa gattcataga ttacctctca
     4741 gtgtaaaacg gaaggttgtg atgaaaccgg caagagtccc tcaaactgtc gtggctcctg
     4801 attgctgggg cgatttgccc tggggagagc tttatcgcaa ggcgctggag cgccagctca
     4861 acccgtggtt cactaaaatg tatggttttc atctgcttaa gattggcaat ttaagcgcag
     4921 aaatcaattg cgaagcgtgc gcggtttctc atcaagtgaa tgtttctgcg caaggaatgc
     4981 ccgtccaggt gcaggcggat ccacttcatc ttccttttgc cgataaatcc gttgatattt
     5041 gtctactggc acataccttg ccgtggtgca ccgatccgca tcgtttatta cgtgaagccg
     5101 atcgggtact gattgatgat ggctggctgg tgattagtgg cttcaacccc atcagtttaa
     5161 tgggattacg caaacttgtg ccggtattgc gcaaaacctc gccctataat agccggatgt
     5221 ttactctgat gcggcaactg gactggctct ctttgttgaa ttttgaagtg ctgcacgcca
     5281 gccgtttcca cgttctcccg tggaacaaac acggaggaaa actattgaat gcgcatattc
     5341 ctgcgcttgg ttgcttacaa cttattgttg cccggaaacg gactattcct ttaacgctaa
     5401 atccgatgaa acagagtaaa aacaagccac gaattcgcca ggcggttgga gccacccggc
     5461 aatgtcgtaa accacaggct taaacttcga cttggtagcc cgtatcttcc agtgtgggat
     5521 tcatcgccgc ggcacgagcc agttcatcac agcgttcgtt ttccgggtgt ccggcatggc
     5581 ctttaaccca ttcccatttg atttgatgct gccccaatgc agcatcaaga cgttgccaga
     5641 gatcgacatt ttttactggt tttttgtctg ctgttttcca gccacgtttt ttccagttat
     5701 ggatccactg ggtgataccc tggcggacat actggctgtc ggtactcaaa atgacttcgc
     5761 aatgttcttt taacgcctcc agcgcgacaa tagcggccat caactccata cggttgttgg
     5821 tggtgcgggt gtagcctgcg ctaaaggttt tctcgcgtcc gcgatagcgt aaaatagcgc
     5881 cgtaaccccc aggtccagga ttacccagac acgaaccatc ggtgaaaatt tctacctgtt
     5941 taagcatctc tggtagactt cctgtaattg aatcgaactg taaaacgaca agtctgacat
     6001 aaatgaccgc tatgagcact gcaattacac gccagatcgt tctcgatacc gaaaccaccg
     6061 gtatgaacca gattggtgca cactatgaag gccacaagat cattgagatt ggtgctgttg
     6121 aagtagtgaa ccgtcgcctg acgggcaata acttccatgt ttatctcaaa cccgatcggc
     6181 tggtggatcc ggaagccttt ggcgtacatg gtattgctga tgaatttttg ctcgataagc
     6241 ccacgtttgc cgaagtagcc gatgagttta tggactatat ccgcggcgcg gagttggtga
     6301 tccataacgc agtgttcgat ataggcttta tggactacga gttttcgttg cttaagcgcg
     6361 atattccgaa gaccaatacc ttctgtaagg tcaccgatag ccttgcggtg gcgaggaaaa
     6421 tgtttcccgg taaacgcaac agcctcgatg cgctatgtgc ccgctacgaa atagataaca
     6481 gtaaacgaac gctgcacggg gcattactcg atgcccagat ccttgcggaa gtttatctgg
     6541 cgatgaccgg tggtcaaacg tcgatggctt ttgcgatgga aggagagaca cagcagcaac
     6601 aaggtgaagc aacaattcag cgcattgtac gtcaggcaag taagttacgc gttgtttttg
     6661 cgacagatga agagctggca gcacatgaag cccgtctcga tctggtgcag aagaaaggcg
     6721 gaagctgcct ctggcgggca taaatacatg taaaaggcgc taaaaatagc gacttgggcg
     6781 atttttgcag caaacgattc aaaagatgag aaaaatcgtt gacgaaggtc gaggcaatcc
     6841 gtaatattcg cctcgttccc aacggaacac aacgcggagc ggtagttcag tcggttagaa
     6901 tacctgcctg tcacgcaggg ggtcgcgggt tcgagtcccg tccgttccgc caatattcag
     6961 aaagcccgaa tcagtaatga ttcgggcttt cgtcgttttg gtgaagtgag aatttttcac
     7021 tccgaagtaa aacggatatg atttaaaaaa tggccagaca ttataaattc tggccacttc
     7081 tgttactcca gcgcatccca tttctccagc agttctactg gaatttcatt tattacctcc
     7141 gagacgcgtt ttccgaccag gcgttctgcc tggcgcagca aaggaatggt cgggctgctc
     7201 ggttcgctgt gctcaaacca gcgacggatg acacgcaggc gttccagcgc gtcgtttcgg
     7261 tcgcggatct gccggggttc tgcgccggtc atcggtgata tttcctccgc gatatgcacc
     7321 ggtagcgcaa tctgctctgc ctgcatctgt tcagtatgaa cgatggtggc gttttccggc
     7381 tgctcagcct gtggttgtgg caggatctcc ggcgtggtgc tctgcactgc tcccggtaac
     7441 agggccagca gttgcgtcag acgggagaag tccggtgcct ggtcattcag cgtttcacgc
     7501 gcatggcgtt gcagccgctc agtggtctct gctgcctggc gaaaggcatc cagcggcagt
     7561 gtgccgcgcg cttccaggtc tgccagttgc tggcgtaccg attctggtgc cagcgcatcc
     7621 gccgggcgta atgcagatag cgaacgctcc acatcccgca cctgaaggcg catggcagca
     7681 ttatttgaca gtgtaatgcc acgaatatcc gccattacac cttcgtggtc cagcaacgcc
     7741 gccagcgcgt tactgcgtgc caccgcagca tcttcggcgg tgatatcttc agtcgcgagc
     7801 agttgaggat ggatggcgtc cgggtacgtg gagcacaacg tttccagcag cgtgagcgcc
     7861 tccgacagtc ccctggcacc ggcctgctgg atacggcagc gcagcaggac aaccagaatg
     7921 cggatatctt tgctgcgcat cagcagacga tgggcgtccc gctcaagctc cgcccagtta
     7981 atgttttccg gcgtactgac aaaatcgcca tattgcgctt cttcacgcgg tgcggcgcgg
     8041 gtgaacagca gcaggtattc cggatcgtac tccagatccg ggccgcaggg gcattccgcc
     8101 gaaattgggt cagacaggaa tatttccatt taattatcct cagtgcgcca ggtactgttc
     8161 tggttcgaaa atcatgcccg tcaccgggtt gtcgtgcatg gctttacccg cccatgcagt
     8221 ccaacccagt tggtacggac caccggtaac agccggaggg gcggcgtggg gctttagctg
     8281 tagctcaatt tcccagcagt attcataacc actaaaggcg cggaccagtt ctgtcaggac
     8341 gggcaggtta ttgctgccag gcagaaaacg tcggtaatcc tccagcgtca gcgggccgat
     8401 aaccagccgg aacttatact gcatgtccgg cacggcctgg ccgatgagtg caccattgcc
     8461 aataacggaa gagggggcag gcatccccag ccgggtgatc tcatcgtccg ccacggctat
     8521 ccagtgcagg acatactcct gtatccggaa aggtacaccg aaatagtgcg ccagcgtggt
     8581 ggccagaccg tccggattgc gggactcacg caccagatgg gccgatgccg ccagacgagc
     8641 gtgatctggc aacgggctgt cagcgctctc ccgcaaatcc tgcccgctga ggctggcgac
     8701 ataaaaggca aagcggtcat gttgcggttt atccagaccg cccccagcgg actgggcgct
     8761 gcgccatgcc tggtaaaact gcgtcagcca gcggtgatga aaaagatcag aaaaacgggt
     8821 cagcgtcggc tttcagtccg gttacgcgcc agttcggtgt agtgcagcgg cagtggaccg
     8881 ttcggccccc acagcccgag gctgtacaga ctgatgtgca ggcgcccttc ctgccagccc
     8941 acactggcga tttctcgtgg cgcaaaggtc agcgcgggcg tttgtccgag gcgaaatttt
     9001 tccatccgtg gttgccaggt gttgcccgcc ggaacggtac acaactgcac atccacgcgg
     9061 cgcatcaggt tcagaaaacc gtaacgccag gggggggcct gcgcctgagt gaaatcgttg
     9121 ctcatacgct tccccgctgt cccgccctga caggccaggt cataattttg ccgcgctgca
     9181 tgctgtgcag cgtcatctgc gagaaggtat taatggaaac atggcgggcg atatagtgtt
     9241 ccagcaccag gccgaacaga tacggactga tgccggaaaa accttcttca tccaccgtca
     9301 gttcacaact gacgccacgc ccatagacca gtagtccgga accgggcaga cggcgggtga
     9361 ccggggtcgt tttacaacca atcaggctgc gcacctggcg cgattgcgga ctgtcatgag
     9421 caggaataaa caggttcagc agatctcgca acgcctggcc gccggtacgg tgatccagat
     9481 cggccagcgg cagataatta aaagataact ggcggatcaa ccgccatgcc atctcacgtt
     9541 cagccagcgg gggctgcggc gagcgtggcg ggcggataag ccctacgccc gccaccggga
     9601 tggctgcatc cacggttaga tcgtcccggc cattgcgggc aataagacag ggcagatcgc
     9661 ggttagtgac catggccgtg atggtgatgt ggcgcagatt ttccgggtac ggcgcttcat
     9721 actggtcaac cagcgacagg aacacttccg agcccgtata gggggtacgg gtgccgtaac
     9781 ggcgggcgtt ttctgataag cggcggggct cccggcgcag ggaaaaataa cgtccgtgat
     9841 tgccttcatc attattgcgg gtgtggtaga gcgggcgaaa ggccatcttc cgtgtggtgt
     9901 cggtttccag cccttccact tcctggaccg agaacacctc ataatccagc ggatgggttc
     9961 tgtccaccac cagatgctgt tccgtcgtgc tgtgcgtgac atcgatccgg gcggtgacgc
    10021 ggggaaacag gttgataacc ggggtacaga acaggctgaa ctgtgccgca tttgtctggt
    10081 ggatcagcca gtccggcggc aggcggttca gcaggataac aatctccgcc acgccaccgt
    10141 caattttctg taatccggca gacaggcctg tcggggtgaa aaaatagaag cgttccggac
    10201 aggcaaaata ttcatgcagc aggttatgac cgtgaaacac gttccacgcc agcggcagca
    10261 gcccctgatc cggctccagg ccttcgtaca taacgggctg ttgcagattc acatccagcg
    10321 cgccgtcaaa atgccccgga atgccagcca gggtcgcgat ggcgctggta tgtagaagtt
    10381 ctaacaggtg tgaggccgta cgctcctcgc cgcagagata aaacggcagc ctgtccagtc
    10441 cggcaagctg actgaacgtt agttcaccaa aggtgcgcag ggtaatacgc agcgccccgg
    10501 cgacgtgaat gttggccggc aggtagcggt gcagggcagg catatccggt ggcgcggcgg
    10561 tcagtcgcgc ctcttcgatg gcgagcggcc acagcgttac atcctggctg ctgcgaaact
    10621 ggcaggcagt gctttcgcct tccggaatgg cggagaaaaa ggctgtgtcg cgcgggacgg
    10681 taaatccttt cgccagatcg ccttcttccg tatcgggatg caactgcgcc acgctcattg
    10741 agggcgtggg tgtgacgtaa ttggggctga ccacttccag caggcgctgg gtgaaacggg
    10801 ggaattcagc gtcaattttt agctgcgtgc gggcggtcag gaagctgaag gcctcgacca
    10861 ttcgctctac atacggatcg gcaatatccg ttccctgcat gcccagccgg gcggcgactt
    10921 tgggatgacg ggcggcaaat tcggctcccg tttcccgcag atagctcagt tcgcggttgt
    10981 agtattccag tagccgtgga tccatcggtc tctctctgaa ttaaaaaaac gtaatgcgac
    11041 tctgctccat atccagcgcg ctacgtaccc ggaatgcagt cggatacggc tgcgtgagga
    11101 tctcgccgcg aatttcaaac tgtaatgtgt tatggccact ctggcggcgc tcatcctgaa
    11161 gtgggatgat ttgcagagtg gacgctttca gccggggttc aaaccgggtg atggttcgca
    11221 aaatggccct gcggatatcg tcccacttgt gctcatacag aaagctgccc gccagtggcg
    11281 gcaggccgta gttaagtacg gacgctgccg cgtacggata acgtgcagca tcaatatcat
    11341 cttcatggct gacggtgttg agcagaaatg acaaatcccg gcggatgatc tccttaatct
    11401 gaaccggact gacgctgata ccgtggtcgc gtttctggtt tggggcgtcg tcacacaggc
    11461 ggtcaaacag cgtgggcagc aggtgattgc ctggacgctg gcgggaagcg ctcacgcgtc
    11521 gcttccttca gcggtgttaa aggtacaggt ggacaaatcc agcaggctga tatcgccgtg
    11581 accggtgagc cacaccctct gacccagcgc ctgtaccagt gtctctcccg gaccgtcctg
    11641 ccagaccgtt tcccggcaca gacgcaggga gtcggcagcc ttttcagagc cgctgtagcg
    11701 gggaaacagc taggcactgt gtgtatcgcc attgttcagg gtgacgttga cgggtttcca
    11761 gatcaaatcg atcagatgcg caggccgcgg cgatttcagc gaacagattt gtgaaaacgg
    11821 cagccagata tatgctccgc ccgcgatcag ttcgagtacc ggaccggtac gggagtcgct
    11881 gtcgctaatc cagtcaaatg cgccgccatt ccactgcccg gaagtgtctg ttatggcctc
    11941 aagcgcgcgg ctgcggtgag catcagcttc ctgcgcttca ccacgggcgt tacaggcgag
    12001 ggcggtcagc agatcctcca tccaggcggg cggcgggaga ataacaccgg gccgctgttc
    12061 gccctgaaag caggcgtggc ggtatatctc acagcggatc agctcgccaa atacctgcgc
    12121 ttcgcgagta taatttgcgt ccatccgggc gcatagctgg atctgttgca gtgcccgcga
    12181 ccagtcgcct gccacgcaca ataactgaaa caggctgtgg cggcgggagg cctgcgctgg
    12241 gtgcatcctg acctgttgct ctgccatctg aatgccctcc accacggaat aatcccgcag
    12301 cagcgccgac aaggtaatcg gtagctcgtt attgtttgtc atgatatatc tctccatgtt
    12361 gtgccgggtt tatccggtaa tggctgtcaa tgccaatgat gcgatgctct ttccgtgtca
    12421 gatctggcag tacggtgtta tgcgctgttt ccggggcgta ttccggggaa agtaaatgca
    12481 ggatatcagg gggagtcttt tcgacaggcc agatagtttc atcttcgcca gtggcatcca
    12541 gtacattgag aatagcgtca atgcttaacc caccagtgac catatcctgc aatgtctcgg
    12601 cttcggtgac caggtcacgc aatgaatcca gatcctgcga ggagggctct tcgttaaaag
    12661 gaaaaggatc agcatggcga ttaagatctt gttcgggaga taacaacgcc tcgcggtatt
    12721 cctgataaag tcgggtcagg gcatttcctt tttccgcacc ttctccggtg gaggaggctc
    12781 catgaacagg aatgatatcg aatggatttt gtgatgtcag atgctgctgc ctgaaccaat
    12841 ccagatccag atattcggtt attgcttccg acgtgatggc agtctgcgat atcgttttta
    12901 ctgtgctcag gttcggagat gatggattgc catgtacaag gcactatgaa gataaccccc
    12961 attctatcag gtcgccttca ttcagacgca tacggtgatg aggttccaca atctggtcgt
    13021 ttaccgtaca gcaatattcg gtagactgat tgataatcca ccatgcttct tcgtggcggg
    13081 tcagagtgag ggcaatcttt acatcatcat tcaccttaaa aacaggaatc cagggagagg
    13141 taacggtgaa gataatttgt gttccttcag tcgcgtaaat tgtgtt
//
LOCUS       DAJVRP010000106        12960 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVRP010000106 DAJVRP010000000
VERSION     DAJVRP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.106"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(1..769)
                     /locus_tag="OQN12_003299"
                     /pseudo
     CDS             complement(1..769)
                     /locus_tag="OQN12_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
     gene            1145..2785
                     /gene="trg"
                     /locus_tag="OQN12_003300"
     CDS             1145..2785
                     /gene="trg"
                     /locus_tag="OQN12_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCS1655516.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2823..3746)
                     /locus_tag="OQN12_003301"
     CDS             complement(2823..3746)
                     /locus_tag="OQN12_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1655517.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3963..5306
                     /locus_tag="OQN12_003302"
     CDS             3963..5306
                     /locus_tag="OQN12_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1655518.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5531..7186
                     /gene="opgD"
                     /locus_tag="OQN12_003303"
                     /gene_synonym="mdoD"
     CDS             5531..7186
                     /gene="opgD"
                     /locus_tag="OQN12_003303"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1655519.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            7326..7550
                     /locus_tag="OQN12_003304"
     CDS             7326..7550
                     /locus_tag="OQN12_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1655520.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7613..8149
                     /gene="rimL"
                     /locus_tag="OQN12_003305"
     CDS             7613..8149
                     /gene="rimL"
                     /locus_tag="OQN12_003305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1655521.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(8144..9124)
                     /locus_tag="OQN12_003306"
     CDS             complement(8144..9124)
                     /locus_tag="OQN12_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655522.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            9248..10240
                     /gene="tehA"
                     /locus_tag="OQN12_003307"
     CDS             9248..10240
                     /gene="tehA"
                     /locus_tag="OQN12_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1655523.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            10237..10830
                     /gene="tehB"
                     /locus_tag="OQN12_003308"
     CDS             10237..10830
                     /gene="tehB"
                     /locus_tag="OQN12_003308"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1655524.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            11133..11801
                     /locus_tag="OQN12_003309"
     CDS             11133..11801
                     /locus_tag="OQN12_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCS1655525.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11819..12325)
                     /gene="tnpA"
                     /locus_tag="OQN12_003310"
                     /pseudo
     CDS             complement(11819..12325)
                     /gene="tnpA"
                     /locus_tag="OQN12_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            12333..12960
                     /locus_tag="OQN12_003311"
                     /pseudo
     CDS             12333..12960
                     /locus_tag="OQN12_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3346 a         3040 c         3224 g         3350 t
ORIGIN      
        1 cccatcaggc gctgaacagc tttatggtta atctgtttcc cttctcgatg aagagacagc
       61 gttaccctac ggtatccgta tcggcctcta ttctcgtgat aaatctcacc aatacgcttt
      121 ttaacgtccg catacttgtc aggcttgctg agagccttta gatggtaata aaacgtactg
      181 cgcggtatct ccgcagccct gagaagctca tcaagaggat aaaactgcct tagctcgttg
      241 agtactttca ctttttcgtg ggatgagcta aggctttcag cttttttaga tacataagcc
      301 gcgtttcaag aaatcgaact tgcctttcaa gatcctcaat gcgtcggtct tttgacagct
      361 ccaatgctga tgccgctttt tctggatcaa ctgatattgc aatgtttctt ttggtgccaa
      421 tcttgagcgc gcgtaaacca gcttctccgc gctcttcata gaccttcagc cacctggcta
      481 cagaaccact accagcaagc ataaagtgag cagcagcctg attaagggac atgtgctgct
      541 cgatcacagc tttcacgacc ttaatacgca actctggatc agcactaacg cctttaggtt
      601 tgggaattaa acctttttct ccatgttttt catagagggc aacccatgtc ctgacctggg
      661 ttcgggggac accaaaacgt gccgagatga tcctgtaacc atcatcagta gtgaagtagt
      721 gattcacgac ttcaaggcgc ttttcaaaag ggtattttgg ctttgacata ttaggggcta
      781 ttccatttca tcgtccaaca aaatgggtgc agtacagttg atagaaatat cggtcggggc
      841 cttcgtcttt ctgattcccg gttagcctga aaacagaaag tctcaggcac ccgcaggcat
      901 cctatgaggt ttccttaggg acgaaaataa tcacttcacg aaattgcgtg ctgttttcca
      961 gaatttttcg tcattcgggt tagccagttt agccattcgt tactctcttc attccaatag
     1021 cattaatttt ctctgcaata attgttgtaa aaatgtgacg caaagaggtt tttggtcata
     1081 agtaattacc gtcaagtgcc gatgactttc tatcaggagt aaacctggac gagagacaac
     1141 ggtaatgaat acaactccct cacagcgatt aggttttttg catcacatca ggctggttcc
     1201 gttatttgcc tgcattcttg gcggtatctt agttctattc gcattaagtt ctgctctggc
     1261 tggctatttc ctctggcagg ccgatcgcga tcagcgtgat gttactgcgg agattgagat
     1321 tcggaccggg ttagcgaaca gttcagattt tttgcgttca gcccggatca atatgattca
     1381 ggccggggct gcgagtcgta ttgcggaaat ggaagcaatg aagcgaaata ttgcgcaagc
     1441 cgaatcggag attaaacagt cgcagcaagg ttatcgtgct tatcagaatc gaccggtgaa
     1501 aacacctgct gatgaagcct tcgacactga attaaatcaa cgctttcagg cttatatcac
     1561 gggtatgcaa cctatgttga aatatgccaa aaatggcatg tttgaagcga ttatcaatca
     1621 tgaaagtgag cagatccgac cgctggataa tgcttatacc gatattttga acaaagccgt
     1681 taagatacgt agcaccagag ccaaccaact ggcggaactg gcccatcagc gcacccgcct
     1741 gggtgggatg ttcatgattg gcgcgtttgt gcttgccctg gtcatgacgc tgataacatt
     1801 tatggtgcta cgtcggatcg tcattcgtcc actgcaacat gccgcacaac ggattgaaaa
     1861 aatcgccagt ggcgatctga cgatgaatga tgaaccggcg ggtcgtaatg aaatcggtcg
     1921 cttaagtcgt catttacagc aaatgcagca ttcactgggg atgacagtag ggactgttcg
     1981 acagggcgcg gaagagattt atcgtggcac cagcgaaatt tcagctggca atgcggacct
     2041 gtcatctcgc accgaagaac aagcggcggc tatcgaacaa actgccgcca gcatggagca
     2101 actcactgcg acggtgaaac agaatgcgga taacgcgcat catgccagca aactggcgca
     2161 agaggcttct attaaagcca gcgatggcgg gcagacggtt tccggtgtag taaaaacgat
     2221 gggcgctatc tccacgagtt cgaagaaaat ttctgagatc accgccgtca tcaacagtat
     2281 tgctttccag acgaatattc tggcactgaa tgctgccgtt gaagccgcgc gagcgggtga
     2341 gcaagggcgt ggatttgccg ttgtcgccag cgaagtacgg acactcgcaa gccgcagcgc
     2401 tcaggcagcg aaagagattg aaggcttgat cagtgaatca gtcaggttaa ttgacctggg
     2461 gtcggatgag gtggcaacgg cagggaaaac catgagcact attgttgatg ccgtcgcgag
     2521 tgtcacacat atcatgcagg aaatcgccgc cgcctcggat gaacaaagta gaggcataac
     2581 tcaggttagc caggcgattt ctgaaatgga taaggtgacg caacagaatg cttctctggt
     2641 agaagaggcc tcagcagcgg cggtgtccct tgaagaacag gctgcacgat taactgaggc
     2701 ggtgaacgta ttccgtctga acaaacattc tgtgtcggca gaacctcgcg gagcgggtga
     2761 accagttagt ttcgctacgg tgtgaaaatg ttcaaggagg gatcgacaga tcccttcacc
     2821 tttcagaacg gcattgattt tcgaatagcg ttaatcatca actggcaacc agaagagaac
     2881 gtcgcatcta cgcgggtcag tattccaatc ggttcgcctg caccatgtcc cggaacaggc
     2941 agggccacca gcgtggcatg acgcaggtcg tcttttacag cgccagaagg gacaaaccac
     3001 acgtaatcgt attcaacggt aagttgacga gatagcgaag tagacagcgt ttcgatacaa
     3061 cccgaaggaa ttttacagcc ctggctctgc actaatgcat ctgaatgctg gcgtggcgca
     3121 gtgccttctg gtgatacaac gaccggccat tccagcaccc ggcttagcgt tacgttctcc
     3181 tgaagtagcg ggtgattagg gcggacaacc agcttcaacg attcaagaaa cagcagttcg
     3241 taattaagcc cggtcatcag ttcaggatct gacatccgac caatgccgat atcgatttcc
     3301 ccggttttta aacccgccag aatcataggg ttactcattg tcgcaacttg caaggtcgtc
     3361 tctttttgtt gctgatgaaa ctgacctata accgaaggta atatccccag tgccgcagta
     3421 ggtagtgcac caaccctgac gacatcatta ttaagacctt ctttacgatg aagcgactgt
     3481 ccggcagtgt tgatggcgtc aagaactctg actgcatgcg ttaaaaattg ttcgccgggt
     3541 aaggtaagtt gcgccccctg acgaccacgc tcaaacaagc gagcgcccgt cagctgctcc
     3601 agttcattca atgtattaga gagcgcaggt tgactcaaat taagggtttc agctgcgcgc
     3661 cccaaagttc cttgttgtgc gacagctacg aatgtatgaa ggtggcgcaa acggatgcgc
     3721 tgactaaaca gactattttt ttccataagc gatgttaaaa acgaagcggt gtcgctgaca
     3781 agtgaagttg tttgattatg ataacttgat tgcaaaatat tattaataat taaagcaatt
     3841 atgttacagc aaaatggata atattgatgt tttcgcggcg agatcacagt ttgtaaattc
     3901 ttcccgccag agtgaatgcg gttacctaca ctccagatta ctgaccactg gaggcagaca
     3961 ctatggcgaa cagcatcacg gcggatgaga ttcgggaaca gttttcgcag gcaatgtcag
     4021 ccatgtacca gcaagaagtt ccgcaatatg gcacgctgct ggaactggta gctgatgtga
     4081 atctggctgt gctggaaaac aatcctcaac tgcacgaaaa aatggtaaat gcagacgagc
     4141 tggcgcgact gaatgttgaa cgtcatgggg cgattcgcgt tgggactgca caagagcttg
     4201 ctactcttcg gcggatgttt gccattatgg ggatgtaccc ggtgagctat tacgatctct
     4261 cgcaggcagg ggtgccggta cattcgacag catttcggcc cattgatgat gcttctctgg
     4321 cgcgtaatcc cttccgcgtt tttacatcct tactccgcct tgagcttatc gagaacgaaa
     4381 ttttgcgcca gaaagcggcg gagattctgc gtcagcgcga tatcttcacc ccacgttgtc
     4441 gacaactgtt agaggaatat gagcagcagg gcggttttaa cgaaacacag gcacaggagt
     4501 ttgtgcagga agccctggaa acgtttcgct ggcaccagtt agcaacggta gatgaagaaa
     4561 cctatcgcgc cttgcacaac gaacatcggt tgattgctga tgtggtctgt tttcctggat
     4621 gccatatcaa ccacctgacg ccacgtacgc tggatattga ccgggtgcag tcgatgacgc
     4681 ctgaatgcgg aattgaaccc aaaattctca tcgaggggcc gccgcgccgc gaggtaccga
     4741 ttttactacg ccagaccagc tttaaagcac tggaagagac ggtgttgttt gcggggcaga
     4801 aacagggcac gcataccgcg cgctttggtg aaattgagca gcgtggcgtg gcattaacgc
     4861 cgaaagggcg acaactgtat gatgatcttc tgcgtaacgc tggaaccggg caggataatc
     4921 tcactcacca aatgcattta caggaaacct tccgcacttt tcctgacagt gagtttttaa
     4981 tgcgtcagca agggttggca tggttccggt accgtctgac gccttcgggt gaggcgcatc
     5041 gtcaggcgat tcatcctgga gacgatccac agcccttaat tgaacgtggt tgggtagtgg
     5101 cgcaacccat cacctatgaa gatttcttgc ccgttagcgc ggcggggatc ttccagtcaa
     5161 atctgggtaa tgaaacgcag acacgcagtc acggtaatgc cagtcgcgaa gcatttgagc
     5221 aggcgttggg ttgtccggtt ttggatgagt tccagcttta ccaggaagcg gaagaacgca
     5281 gtaaacgtcg ctgtggtttg ctttaaattc tgaccatccg cctttgcaaa aatttgcctg
     5341 attttacaaa cgaatcaggc tcatcccatc gacataaaaa aaatgccgat ttatgcatat
     5401 tctctcagtt caacaattgg attattaata aatattgtct agagtgagcg gtcataaata
     5461 agcactttct tgccgctgaa aacgaccagc gcgggaccat tcacaacacc agaaggactc
     5521 actttcaggt atggatcgta gacgatttat taaaggttta atggctatgg ccgccgtgtg
     5581 cggtaccagc ggcattgctt ctcttttttc tcaggcagca ttcgcggcag attctgatat
     5641 tgccgacggg caaacccagc gttttgactt ctccattcta cagtcaatgg cgcacgactt
     5701 agcgcaaaca gcgtggcgtg gtgcgcctcg tccgttacct gacacgctgg cgacaatgac
     5761 gccgcaggct tataacagta ttcaatacga cgccgaaaaa tcgctctggc ataacgttga
     5821 gaaccgtcaa ctggacgctc agttcttcca tatgggaatg ggattccgtc gccgcgttcg
     5881 tatgttttct gtagatccag caacacatct ggcgcgtgaa attcactttc gcccggagtt
     5941 gttcaaatac aacgatgcag gtgttgatac caaacaatta gaagggcaaa gcgatctcgg
     6001 ctttgccggt tttcgcgtgt ttaaagcccc cgaactggcg cgccgtgatg tagtatcatt
     6061 tctcggcgcg agttatttcc gcgccgttga tgatacatat caatacggtt tgtcggcccg
     6121 cggcctggcg atcgacactt acaccgacag taaagaagag ttccccgact ttaccgcctt
     6181 ctggtttgat acggtaaaac cgggggcaac tacctttacc gtttatgcgt tgctcgatag
     6241 cgccagcatt actggtgcct ataagttcac tatccattgt gagaaaagtc aggtgattat
     6301 ggatgtggaa aatcacctgt atgcgcgcaa agacattaaa cagctgggca tttcgccgat
     6361 gaccagtatg ttcagctgcg gtactaatga acgtcggatg tgcgatacaa ttcatccgca
     6421 aattcatgac tctgatcgtc tgtccatgtg gcggggcaac ggcgagtgga tttgccgtcc
     6481 gctgaataat ccgcaaaaat tgcagttcaa tgcttacacc gacaacaacc cgaaagggtt
     6541 tggtttattg caactggatc gtgacttctc ccattatcag gacattatgg gctggtataa
     6601 caaacgccca agtctgtggg tggaaccgcg taacaagtgg ggtaagggca ccatcggcct
     6661 gatggaaatc ccaacaacgg gcgaaacgct ggataacatt gtctgcttct ggcagccaga
     6721 aaaagctgta aaagcaggtg atgagtttgc attccagtat cgtctgtact ggagtgcgca
     6781 accgcctgtt cattgcccat tagcgcgcgt tatggcgacg cgtaccggca tgggcggttt
     6841 cccggaaggt tgggcgccag gtgaacacta tcccgaaaaa tgggcgcgtc gttttgccgt
     6901 cgatttcgtt ggtggtgatc tgaaagctgc cgcgccaaaa ggcattgagc cggtgattac
     6961 gctttccagt ggggaagcga agcaaatcga aattctctat attgaaccca tcgatggtta
     7021 tcgtattcag tttgactggt atccgacttc ggactccact gatccggtcg atatgcggat
     7081 gtatctacgt tgtcaggggg acgctatcag tgaaacatgg ctgtatcagt atttcccgcc
     7141 agcgccggat aaacgtcagt atgttgacga ccgcgtgatg agttaatcgt tttttcttcg
     7201 gcaccttctt cgggaggtgc cgtctggtta aacacgatcc cgctcgcatt tttccctaag
     7261 ttaaatgagt aatctgatgg tgtgtatttc agatacaccg tgtcagccac taacagggag
     7321 tgcgtatgtt tccagaatac cgagatttaa tatcccgtct gaaaaacgaa aatcctcgct
     7381 ttatgtcctt gttcgataaa cacaataaac ttgatcatga aattgccaga aaggaaggtt
     7441 ccgacggtcg agggtacaat gcggaagtgg tccgcatgaa aaaacaaaag ctacagttaa
     7501 aagatgagat gctcaaaatc ctgcagcagg agagcgtcaa agaggtgtaa actttcctaa
     7561 gccgccagcc aggcggcttt tttaacaaca gcatggattg actggagata agatgactga
     7621 aacgataaaa gtaagcgaat cacttgaatt acatgctgtt gcggaaagtc acgtcacacc
     7681 tctttatcag ttaatctgta aaaataaaac ctggttacag cagtcgctaa actggccgca
     7741 gttcgttcag agtgaagagg acacgcgaaa aacggtgcag ggtaatgtga tgttgcatca
     7801 acgcggctat gccaaaatgt tcatgatttt caaagaagat gaacttatcg gcgttatctc
     7861 gtttaatcgt attgaaccac tgaataaaac tgctgaaatc ggctactggc tggacgaatc
     7921 tcatcagggg caggggatca tttctcaggc gctgcaggca ttgattcatc attacgctca
     7981 gtctggtgaa cttagacgct tcgtgatcaa atgtcgggtg gacaatccgc aaagcaacca
     8041 ggtcgctttg cgcaatggtt ttatccttga aggttgcctg aaacaggctg agttcctgaa
     8101 tgatgcctat gatgatgtga acttatacgc gcgtattatc gattcataat aaccctgcca
     8161 gcggcgttcg cgtaatgcgc tcctcgccgt taatgacttt tggaccacgg acatgaaccg
     8221 tgtcgccatc aaaggcttca acgacggcgt ggtcggttaa ctccacatgg ttttcaatta
     8281 tcaccgcacc tgtaatacgg ctctcacctt gtattacaac gtgctcgtcg agcagaatcg
     8341 gtccaccacg taccacggca tttccgccaa tcaagacatg atgttttaac acacaattac
     8401 cttccacaat ggcatattcc gccacctgcg aactgtaatg aatcgtcgga atggcatctt
     8461 cttctatgcc agctttcacc tgcgcgtgac cgtagacttt agcgcaatcg catagccaga
     8521 cgttgttctc ttcattacct tcaacactgg caaaatcaaa aacttcggcg cgatgttcaa
     8581 taaaagcata ccggacgacg gcatcgccat aaatttgtgc ctggtggaca atacgcgagg
     8641 cgctaaccct tgcgcgatca taaatttgga gcaggagttg atggtcgggc gttaagcctt
     8701 gtgcggcgac gatcatagaa tgttgatcaa ttaaggcgtg accaaatatt ctacattgca
     8761 cgtaaactaa tgagtcatga atggtgacac tgtcacttat atatgcaccc tggctgattt
     8821 cactattatc gatccagacg ttatccgttg cgtaaacctc tccccacagc acgctggtgc
     8881 cggtgatacg cgtattaccg gaaatcaccg ttccgccgaa cgcaatagca ttctggtcgt
     8941 aaatccagca atttccttcc tgtgctaaca ccgtttcgcg atcgatccag ccgccagcgg
     9001 ttcctgctat cacatcgtta aaatcgctaa tggcgataat ctgccgtaat aacacacttt
     9061 ttttagtgcc atcttcctga taactaaagg ctcgctgttc ttcacttagg cgatatttgc
     9121 gcatagcgtt ttcccacagg tgacttacta taaccgtagc aaattctgcg gctctggcta
     9181 tgctcgagaa aattccataa aatgcatttc aaatatactt tataaattaa acaaaatgag
     9241 taagaagatg cagagcgata aagtgctcaa tttgccggca ggctactttg gtattgtgtt
     9301 ggggacgata gggatgggat ttgcctggcg ctatgccagc caggtttggc aggtcagcca
     9361 ctggttaggg gatgggctgg tgattctggc gatgatcatc tggggattat tgactagcgc
     9421 atttattacc cgactcatac gctttccgca tagcgtgctg gcggaagttc gccatccagt
     9481 gctgagcagt tttgtgagtt tgtttccggc aacgacgatg ctggtggcga ttggttttgt
     9541 tccgtggttt cgcccactgg cggtgtgcat gttcagtttt ggtgtcgtgg ttcagttggc
     9601 ttatgccgcc tggcaaactg cgggattatg gcgcggatct caccctgaag aagctaccac
     9661 gcctggactg tatctgccga cagttgccaa caactttatc agcgcaatgg cctgtggtgc
     9721 gttgggctac accgacgccg gtctggtgtt tttaggcgca ggcgttttct catggctaag
     9781 ccttgaaccg gtgatcttgc agcgtctgcg cagttcggga gaattaccca cggcactgcg
     9841 gacatcactc ggcattcagc tcgctcctgc gctggtggcc tgtagtgcct ggctgagcgt
     9901 caacggcggc gagggtgaca cgctggcgaa aatgcttttc ggttatggac tgctgcaact
     9961 gctgtttatg ctacgtctga tgccatggta tctctcccag ccatttaatg cttcattctg
    10021 gagtttctcg ttcggcgtat ctgcactggc aaccaccggt ttgcatctgg ggagtggcag
    10081 cgataatgga tttttccata cgctggcggt gccgctgttt atctttacca attttattat
    10141 tgcaatactg ctcatccgta cttttgcgct tctgatgcag ggaaaattgt tagtcagaac
    10201 cgagcgcgcc gttttaatga aagcagagga caaagaatga tcattcgtga cgaaaactat
    10261 tttactgata aatatgaatt aacccgcaca cactctgaag tactggaagc ggtgaaagtg
    10321 gttaaaccgg gtaaaacgct ggatctgggc tgtggcaatg gtcgtaacag tctttacctg
    10381 gcagccaatg gttatgatgt tgacgcatgg gataaaaatg ccatgagtat cgccaacgtc
    10441 gagcgcatta aatccattga aaatctggat aatttacaca cccgggtcgt tgatctgaat
    10501 aacctcacat ttgatggaca gtacgatttt attctttcga ctgtggtgct gatgttcctt
    10561 gaggctaaaa ccatcccagg gttgattgcc aatatgcaac gttgcactaa acctggtggt
    10621 tacaacctga ttgtggcggc gatggatacc gctgattatc catgtaccgt cggcttcacg
    10681 tttgccttca aagagggaga attacgtcga tattacgaag gctgggagag ggtgaaatac
    10741 aatgaagacg tcggcgagct gcaccgcacc gacgccaacg gtaatcgtat taaactgcgt
    10801 ttcgccacga tgctggcacg taaaaaatga cccggtaagc acaaaacgcg tgaaaattcc
    10861 ccacgctgag atgatttact gttcttcttt tcggtaagca tattttttat cgaagggatg
    10921 tgaaattaat cacagtagtc gaagtttttt agcagcttaa cttactgaaa tttaactact
    10981 gatgattgac ttagcccctt tttcggcatt gactatgtcg tctgaaaagg ggctgaaaaa
    11041 tttattttca ccaacacttt ttttgccaca acacgaagcg gcgctttttg ctataactta
    11101 gaaagtaata taatcatctc aggaaactat tcatgcgtac cacatcattt gcgaaagttg
    11161 cagctttatg cggcttgttg gctctgtctg gttgtgcctc taaaatcacc cagccagata
    11221 aatattctgg ttttttaaac aattactctg atttaaaaga aacaacctca gcaacaggta
    11281 aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac agcatcgtct
    11341 ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg cagaaagttc
    11401 tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg cagcgtaaac
    11461 cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt accggtgtag
    11521 ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta gtggttgcgg
    11581 ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt gaaggtgagc
    11641 tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc gaaggtaaag
    11701 acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt attgatgaca
    11761 tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg cgccatcgtt
    11821 agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg ggatataagg
    11881 cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg atgacggata
    11941 ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg cctttgtagt
    12001 aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat acgcctttga
    12061 ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac aaatggacgt
    12121 gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat acatttgaaa
    12181 agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg tatctggtga
    12241 caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg atatcggttt
    12301 cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca tttaaattcc
    12361 agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc gcatgtcgtt
    12421 tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca tgaggccggt aataaataca
    12481 tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact gaaacgcaat
    12541 ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt gagcgggctt
    12601 acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg tgacagaatg
    12661 atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt atttttctgc
    12721 cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt gtgaaaaatg
    12781 tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa agtgaagtat
    12841 ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg gctaaactcg
    12901 ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa aaccagaaga
//
LOCUS       DAJVRP010000107        12906 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVRP010000107 DAJVRP010000000
VERSION     DAJVRP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.107"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OQN12_003312"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="OQN12_003312"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS1655526.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3223)
                     /locus_tag="OQN12_003313"
                     /pseudo
     CDS             complement(1806..3223)
                     /locus_tag="OQN12_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(3377..3721)
                     /locus_tag="OQN12_003314"
     CDS             complement(3377..3721)
                     /locus_tag="OQN12_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCS1655527.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3721..4647)
                     /gene="glsB"
                     /locus_tag="OQN12_003315"
     CDS             complement(3721..4647)
                     /gene="glsB"
                     /locus_tag="OQN12_003315"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1655528.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4711..5646)
                     /locus_tag="OQN12_003316"
     CDS             complement(4711..5646)
                     /locus_tag="OQN12_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCS1655529.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5726..6841
                     /gene="yneK"
                     /locus_tag="OQN12_003317"
     CDS             5726..6841
                     /gene="yneK"
                     /locus_tag="OQN12_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCS1655530.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6991..8181
                     /gene="ydeA"
                     /locus_tag="OQN12_003318"
     CDS             6991..8181
                     /gene="ydeA"
                     /locus_tag="OQN12_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1655531.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8206..8871)
                     /gene="marC"
                     /locus_tag="OQN12_003319"
     CDS             complement(8206..8871)
                     /gene="marC"
                     /locus_tag="OQN12_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1655532.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9083..9517
                     /gene="marR"
                     /locus_tag="OQN12_003320"
     CDS             9083..9517
                     /gene="marR"
                     /locus_tag="OQN12_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1655533.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9537..9920
                     /gene="marA"
                     /locus_tag="OQN12_003321"
     CDS             9537..9920
                     /gene="marA"
                     /locus_tag="OQN12_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS1655534.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9952..10170
                     /gene="marB"
                     /locus_tag="OQN12_003322"
     CDS             9952..10170
                     /gene="marB"
                     /locus_tag="OQN12_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1655535.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10201..11100)
                     /gene="eamA"
                     /locus_tag="OQN12_003323"
     CDS             complement(10201..11100)
                     /gene="eamA"
                     /locus_tag="OQN12_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCS1655536.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11364..12482
                     /gene="ydeE"
                     /locus_tag="OQN12_003324"
     CDS             11364..12482
                     /gene="ydeE"
                     /locus_tag="OQN12_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCS1655537.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12609..12704
                     /gene="mgtS"
                     /locus_tag="OQN12_003325"
     CDS             12609..12704
                     /gene="mgtS"
                     /locus_tag="OQN12_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCS1655538.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3399 a         3296 c         2951 g         3260 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaatca tcggtcagcg
     1921 ttcggtacga tgcgcgacca ctaccgacgc caatacttac cgtgagataa agcgtttttt
     1981 gttgccaggt gaatggttgc agttcaacgc ctttacggat tttttccgcc attagcagac
     2041 catcgacagg attcaccgac ggcactgcaa cagcaaattc ttcgccgccc atgcgcgcca
     2101 ccagcccctt atcaccgaca atcttctgaa tatgccgggc aaacacgctt aacactttat
     2161 cgccacattc atgtccatag ttatcgttaa tgcttttgaa atagtcgata tcaagcagca
     2221 tgaccgtcag atgttgtgtc tgtttcagcg atggactttt cagcgcctca taaagaccgg
     2281 accgtgagta aacctgagtc agaaaatcaa agtcggctcg cagcgcaact tgcttcatta
     2341 gcaaattgat cgctgccacg ctaaaagaaa ccataattgg gcatatcgcc atcgtggcaa
     2401 taccgagacg tgcggagaac atttccggaa tggagaacgg cgaaccgacc gagatatcaa
     2461 tcaccgaatt tgccaccagt acgatttcca ccgcaccggt gacaaaggtc aacagacatg
     2521 ttacctgcgg cgtatagcgc actgcacacc agattaatgc aggcagcggg aacgccagac
     2581 tccccgctcc gccaatgact accgaggcaa taaccgacac aataagcgcg atggcgggca
     2641 tcatctgttc tgctttaaag cgcggcagca ctccaggaat agccaacgtc agcatacaag
     2701 gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga
     2761 aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg
     2821 caatcagaca gtaattaaat agccgtaaag cacttactgg ctcatactta tttttcccca
     2881 gacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat
     2941 tgataaccag tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca
     3001 cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca aacacccccg
     3061 ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac
     3121 caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac
     3181 cttcctggaa caaacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac
     3241 aattgcgggt ggcaattatc tcaacgtgat aagtaaattc tcataaagga tagcatgttc
     3301 gctaacagcg gcggcaaata aaggtaccct gggaatgcta ttttatcgtt ttccgcaatt
     3361 ctcatggctg attcatctat acaaccagcc agtgatagat taccgccacg atataacgtt
     3421 gttgttcttc gcttaacgaa acaccctgcg gaatgttatg ccacttagcc agacaaccgc
     3481 gacagcaggt cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt
     3541 gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat
     3601 gttgttcgat gactggcgcg cctttctcca ggcaatactg acgctctttc atgcccagac
     3661 gaaagcgaga gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agatactgca
     3721 ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcga tacccgcaag
     3781 cgagttacct gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac
     3841 aatcgccaca ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa
     3901 ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt gccgcgcctg
     3961 cattggcgtc accacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac
     4021 aaacgtccgg gccagctcta cacagctcat tttcagagcg cagtaatgaa agtagttttg
     4081 cagaacggtt gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc
     4141 cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt atgaaatatc
     4201 agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttagtcg
     4261 cccttgcaac atatcgcaga ccaccagcgc accggtatta atgaacggat tacgcggaat
     4321 accctgttcc atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc
     4381 gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac
     4441 tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc
     4501 gtcaacggta cagatagcaa tccccaatcg ggaaccgtct actgtagcca gcgccggaat
     4561 ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa
     4621 aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc
     4681 tgcgagtata tcagagctga atatgtcgcg tcagatccgg tctttccaca ctgtctggat
     4741 attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac
     4801 gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca
     4861 ttccagacgt gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa tggtcgctga
     4921 aaggccgaac tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaatggt
     4981 gattgcagca acggggccaa acatttcttc ccgaaacgcg gtcatttctg gggtaacatt
     5041 cgccagaacc gttggcggat agtagttacc tgctccagcc atcttttccc cgcccagtaa
     5101 caaacgcgca ccctgcgcca gggttttctc cacctgatga tgcagctcat cacgtaaatc
     5161 aaaacgagcc attggtccga gagcgttctc ttcatcacgg ggatcgccca ttttcaaggc
     5221 tgccgcagct gacacaaaac gttcggtaaa tgccgaagca attccctctt cgataataaa
     5281 gcgttttgcc gctgcacaaa cctgtccggt attctgataa cgtccggcta ccgccgcttt
     5341 aaccgccagt tccagatcgg catcgttaag cacaataaat ggatccgaac cgcccagttc
     5401 cagtacgcat tttttcagtg ccgctccagc acgaacactt ccggtcaccg tgacagcagc
     5461 aatgcgcgag tctttaatca tctgactgac accgtcgttg tcggcattca gccagccata
     5521 tacgccttgc gggattcccg catctttaaa cacttgggca atgagctgag cacagcccat
     5581 cacattcggc gcatgtttaa gtaagtagcc gttacctgca agaatgatgg gaacagcgcc
     5641 acgcatcacc tgcctttgat agttcttgca gctcaaatgc cctatatagt gatgttccga
     5701 tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc
     5761 tcttcctgat gattttcccg ctcgtaatat tgcacctcaa gtaaaagaag ttttaaagga
     5821 ttttattgat gcactcagta ccataatatg tgatgaagag tggcgcacgt ctttaaacac
     5881 caactcagcc acaaaaaaga tatttaacaa ccttgataac ttatcatata ttcagaggac
     5941 ttcttttcgg ggtaacgaca cactatacaa tgaaaaggtt cagtttaaac tcacttatcc
     6001 tgtcaagaat ggaagacaca aagaaaatat tgaatttcag gtagtaataa atttaagccc
     6061 tatttattta gataattttc gccatgatgg agaaattaat attttttgcg ctcccaaccc
     6121 aaagcctgtc actatggggc gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt
     6181 tatgaatgat tttattgaac aatttccaat gatcaacctt ggcgccccca tcaaaagagc
     6241 gcatacacca catattgaac ccctgcctcc ggatcaccat accgctgcag attacttacg
     6301 ccagtttgat ttgcttgtcc tgaattttat ctctcgcggt aattttgtca tactcccccg
     6361 attatggaat aactctgagg ttcacagatg gttcgtcaat aaagatccta acttgatcac
     6421 cgccattctc gacataacgg acagcgaatt aaaagaggat ttattgcaaa gcctgatgga
     6481 ttcattaggt tctaacaaac atgtacaacc cgaagtctgc atctgcttct tatccctttt
     6541 agcagaacaa gaatctcctc attttcagga cttgttttta tttttcgcca atatgttatt
     6601 gcactatcac caatttatga atcccaatga aagtgatctg aatgacgtgt tgatgccagc
     6661 atcattaagt gatgataaaa ttatcaaaca tatggcgcgc aggaccctca aactgtttgt
     6721 aaaaaatgaa acgccgccaa aagttactca cgaagacctg gtgaaaaaca ggcctcgctc
     6781 ccctgtcaga ccacctatac ccgcaaccgc caaaacgcca gacctccctg aacgtcatta
     6841 aaccgtgatg ttaccgactc tctgactcgg aacagaatca gcgtcagaga aacggaaaac
     6901 gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc
     6961 actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg
     7021 gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt
     7081 gtccctgttg gcctgctctc tgacattgcg caaagttttc acatgcaaac cgctcaggtc
     7141 ggcatcatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta
     7201 atgaccagtc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc
     7261 agccacgtac tgtcgttttt atcgtggagc tttaccgttc tggtgatcag tcgcattggt
     7321 gtggcttttg cacatgcgat tttctggtcg attacggcgt ctctggcgat ccgtatggct
     7381 ccggccggga agcgggcaca ggccttgagt ttaattgcca ccggtacagc actggcgatg
     7441 gtcttaggtt taccgctcgg gcgcattgtg gggcagtatt tcggctggcg aatgaccttc
     7501 ttcgcgattg gtattggggc gcttgttacc cttttgtgcc tgattaagtt acttccctta
     7561 ctgcctagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg ccgcccggca
     7621 ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc
     7681 tatatcgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta
     7741 ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat
     7801 caatatgcgt ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg tctggcactg
     7861 ctgctacctg cggcgaacag tgaaatacac ctcggggtgc tgagtatttt ctgggggatc
     7921 gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgct
     7981 accgacgtcg cgatggcgct attctccggc atatttaata ttggaatcgg ggcgggtgcg
     8041 ttggtaggta atcaggtgag tttgcactgg tcaatgtcga tgattggtta tgtgggcgcg
     8101 gtgcctgctt ttgctgcgtt aatatggtca atcattatat ttcgccgctg gccagtgaca
     8161 ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat ggtacgtttt
     8221 aatgatttcc aggatgccgt taataataaa ctgcacaccc atacatacca gcaggaaccc
     8281 catcaggcgg gagatcgctt caatgccacc cttgcccacc agccgcataa ttgcgccgga
     8341 gctgcgtagg cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac
     8401 catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg agctaataat
     8461 catcgctatg gttcccggac cggcggtact tggcattgcc agtggtacaa aggcaatatt
     8521 ggcactgggt tcatcttcca gctcttccga cttgcttttc gcctccggtg aatcaatcgc
     8581 tttctgttgc ggaaaaagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat
     8641 tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccag cgtaatacgc
     8701 caccatcatg atggcaaata cgtacaccga ggccatcaac gactgacgat tacgttcggc
     8761 actgttcatg ttgcctgcca ggccaagaaa tagcgcgaca gttgttaatg ggttagctaa
     8821 cggcagcaac accaccagcc ccaggccaat tgctttaaac aaatctaaca ttggtggttg
     8881 ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaagg ataaagtgtc
     8941 actctttagc taaccttgca tcgcattgac caaaacttga accgatttag caaaacgtgg
     9001 catcggtcaa ttcattcatt tgacttatac ttgcctgggc aatattaccc cctacaacta
     9061 attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc
     9121 attgggtcgc ttaatccata tggttaatca gaagaaagat cgtctgctta acgagtatct
     9181 gtctccgctg gatattaccg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc
     9241 gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag cactgacccg
     9301 tatgctggat cgcctggtct gtaaaggctg ggttgaaagg ttgccgaacc cgaatgataa
     9361 gcgcggcgta ctggtaaaac ttaccaccag cggcgcggaa atatgcgaac aatgccatca
     9421 attagttggc caggacctgc atcaagaatt aacaaaaaac ctgacggcgg acgaagttgc
     9481 aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaagaggt atgacgatgt
     9541 ccagacgcaa tactgacgct attaccattc atagcatttt ggactggatc gaggacaacc
     9601 tggaatcgcc actgtcactg gagaaagtgt cagagcgttc gggttactcc aaatggcacc
     9661 tgcaacggat gtttaaaaaa gaaaccggtc attcattagg ccaatacatc cgcagccgta
     9721 agatgacgga aatcgcgcaa aagctgaagg aaagtaacga gccgatactc tatctggcag
     9781 aacgatatgg cttcgagtcg caacaaactc tgacccgaac cttcaaaaat tactttgatg
     9841 ttccgccgca taaataccgg atgaccaata tgcagggcga atcgcgcttt ttacatccat
     9901 taaatcatta caacagctag ttgataacgt gacaacgtca ctgaggcaat catgaaacca
     9961 ctttcatccg caatagcagc tgcgcttatt ctcttttccg cgcagggcgt tgcggaacaa
    10021 accacgcagc cagttgttac ttcttgtgcc aatgtcgtgg ttgttccccc atcgcaggaa
    10081 caaccaccgt ttgatttaaa tcacatgggt actggcagtg ataagtcgga tgcgctcggc
    10141 gtgccctatt ataatcaaca cgctatgtag tttgttctgg ccccgacatc tcggggctta
    10201 ttaacttccc acctttaccg ctttacgcca ccgcaagcca aatacattga tatacagccc
    10261 ggtcataatg agcaccgcac ctaaaaattg cagacccgtt aagcgttcat ccaacaatag
    10321 tgccgcactt gccagtccga ctacgggcac cagtaacgat aacggtgcaa cccgccaagt
    10381 ttcatagcgt cccagtaacg tcccccagat cccataacca acaattgtcg ccacaaacgc
    10441 cagatacatc agcgacaaga tggtggtcat atcaatagta accagactgt gaatcatgga
    10501 tgcggaacca tcgaaaatca gcgagacaac aaagaaggga atgattggga ttaaagcgct
    10561 ccagattacc agcgacatca ccgccggacg cgttgagtgc gacatgatct ttttattgaa
    10621 gatgttgcca cacgcccaac taaatgctgc agccagggtc aacataaagc cgagcatcgc
    10681 cacatgctga ccgttcagac tatcttcgat taacaccagt acgccaaaaa tcgctaaggc
    10741 gatccccgcc aattgtttgc catgcagtcg ctccccgaaa gtaaacgcgc caagcacgat
    10801 agtaaaaaac gcctgtgcct gtaacaccag cgaagccagt ccagcaggca taccaaagtt
    10861 aatggcgcag aacagaaagg caaactgcgc aaaactgatg gttaatccat accccaagag
    10921 caaattcagt ggtactttcg gtcgtgcaac aaaaaagatg gccggaaaag cgaccagcat
    10981 aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt tgatgaccac
    11041 aaaatttagc ccccatacga ccactaccag tagcgccaac accccatctt ttcgcgacat
    11101 tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt tacttttctt
    11161 taacagttga ttcgttagtc gccggttacg acggcattaa tgcgcaaata agtcgctata
    11221 cttcggattt ttgccatgcg tccgatggtt aataacaacg acgaggcaag aagggcgctg
    11281 gtagagcgtc gtagggataa gttcatttgt ctgtccggca gtgcgtttcg ttatgtttta
    11341 ttttagagat gtaaagaaat agcatggcta cgctgccatt tatgaccatt tacttgagtc
    11401 gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg ctcactattg
    11461 gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag aaacgctata
    11521 tgttactggc aattaccgcc ttcgccagcg gttttattgc cattccttta gtgaataacg
    11581 tgacgctggt tgtgctcttt tttgccctca ttaactgcgc ctattctgtt tttgctactg
    11641 tgctgaaagc ctggtttgcc gacaatcttt cgtccaccag caaaacgaaa atcttctcaa
    11701 tcaactacac catgctaaac attggctgga ccatcggtcc gccgctcggc acgctgttgg
    11761 tgatgcagag catcaatctg cccttttggc tggcagctat ctgttccgcg tttcccatgc
    11821 ttttcattca aatatgggta aagcgcagcg agaaaatcat cgccacggaa acaggcagtg
    11881 tctggtcgcc gaaagtttta ttacaagata aagcactgtt gtggtttacc tgctctggtt
    11941 ttctggcttc ttttgtaagc ggcgcatttg cttcatgcat ttcacaatat gtgatggtga
    12001 ttgctgatgg ggattttgcc gaaaaggtgg tcgcggttgt tcttccggtg aatgctgcca
    12061 tggtggttac gttgcaatat tccgtgggcc gccgacttaa cccagctaac atccgcgcgc
    12121 tgatgacagc aggcaccctc tgtttcgtca tcggactggt cggttttatt ttttccggca
    12181 acaacctgct attgtggggt atgtcagctg cggtatttac tgtcggtgaa atcatttatg
    12241 cgccgggcga gtatatgttg attgaccata ttgcgccgcc aggaatgaaa gccagctatt
    12301 tttccgccca gtctttaggc tggcttggtg ccgcgattaa cccattagtg agtggcgtag
    12361 tgctaaccag cctgccgcct tcctcgctgt ttgtcatctt agcgttggtg atcattgctg
    12421 cgtgggtgct gatgttaaaa gggattcgtg caagaccgtg ggggcagccc gcgctttgtt
    12481 gatttaagtc gaacacaata aaaatttaat tcagccttcg tttaggttat ctctgctaat
    12541 atctttctca ttgagatgaa aattaaggta agcgaggaaa cacaccacac cataaacgga
    12601 ggcaaataat gctgggtaat atgaatgttt ttatggccgt actgggaata attttatttt
    12661 ctggttttct ggccgcgtat ttcagccaca aatgggatga ctaatgaacg gagataatcc
    12721 ctcacctaac cggccccttg ttacagttgt gtacaagggg cctgattttt atgacggcga
    12781 aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg ttttgctttt
    12841 acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc atcagttttc
    12901 tcaatg
//
LOCUS       DAJVRP010000108        12886 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVRP010000108 DAJVRP010000000
VERSION     DAJVRP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.108"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            502..939
                     /locus_tag="OQN12_003326"
                     /pseudo
     CDS             502..939
                     /locus_tag="OQN12_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382442.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1178..1237
                     /locus_tag="OQN12_003327"
     CDS             1178..1237
                     /locus_tag="OQN12_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1655539.1"
                     /translation="MMKLVIILIVLLLASFAAY"
     gene            1554..1613
                     /gene="ibsE"
                     /locus_tag="OQN12_003328"
     CDS             1554..1613
                     /gene="ibsE"
                     /locus_tag="OQN12_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS1655540.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1733..3166)
                     /gene="hldE"
                     /locus_tag="OQN12_003329"
     CDS             complement(1733..3166)
                     /gene="hldE"
                     /locus_tag="OQN12_003329"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1655541.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3214..6054)
                     /gene="glnE"
                     /locus_tag="OQN12_003330"
     CDS             complement(3214..6054)
                     /gene="glnE"
                     /locus_tag="OQN12_003330"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1655542.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6077..7378)
                     /gene="ygiF"
                     /locus_tag="OQN12_003331"
     CDS             complement(6077..7378)
                     /gene="ygiF"
                     /locus_tag="OQN12_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1655543.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7620..8240
                     /locus_tag="OQN12_003332"
     CDS             7620..8240
                     /locus_tag="OQN12_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1655544.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8304..9542
                     /gene="cca"
                     /locus_tag="OQN12_003333"
     CDS             8304..9542
                     /gene="cca"
                     /locus_tag="OQN12_003333"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1655545.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9705..10526)
                     /gene="bacA"
                     /locus_tag="OQN12_003334"
     CDS             complement(9705..10526)
                     /gene="bacA"
                     /locus_tag="OQN12_003334"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1655546.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10617..10985)
                     /gene="folB"
                     /locus_tag="OQN12_003335"
     CDS             complement(10617..10985)
                     /gene="folB"
                     /locus_tag="OQN12_003335"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1655547.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11090..11707
                     /gene="plsY"
                     /locus_tag="OQN12_003336"
     CDS             11090..11707
                     /gene="plsY"
                     /locus_tag="OQN12_003336"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1655548.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11720..12651)
                     /gene="ttdR"
                     /locus_tag="OQN12_003337"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11720..12651)
                     /gene="ttdR"
                     /locus_tag="OQN12_003337"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         3106 a         3627 c         3213 g         2940 t
ORIGIN      
        1 ggagtcatta ccgaacaaca agaagcgttt atgacgcttg agcaggagca gcaggttaaa
       61 acccgtactg ccgaacagaa tgcacgtatt gcggcttttg aagctgaacg tcgtcgtgaa
      121 gcagagcaga cgcgaattct ggctgaacga caaattcagg aaacggagat cgagcgcgag
      181 caggccgtcc gctcaagaaa ggttgaggct gaacgtgaag ttcgtattaa agagatcgaa
      241 cagcagcagg tcaccgaaat cgccaaccag acgaaatcga tcgctattgc cgccaaatcg
      301 gaacaacagt cccaggcaga agcgcgtgct aaccttgcac tcgcagaagc agtaagtgcg
      361 caacaaaacg tagaaaccac tcgccagacc gcagaagccg atcgtgctaa acaagttgcc
      421 ctaatcgctg ccgcgcagga tgcagaaacc aaagcggttg aactgaccgt gcgggcgaaa
      481 gcagaaaaag aagccgcaga gatgcaggcg gcggctatcg ttgagttagc cgaagctaca
      541 cgtaaaaagg gtctggcgga agcagaagca caacgtgcgc tgaacgatgc tatcaacgta
      601 ctttctgatg aacaaaccag ccttaaattc aaactggcct tgttgcaggc gctacctgcg
      661 gtaatagaaa aatccgttga gccgatgaaa tctattgacg gtatcaagat tatttaggtc
      721 gatggtctga atagtggcag cgctgcgggt gatgcaaaca cgggtaatgt ggagggcgga
      781 aacctagcgg aacaagcatt atcagccgct ctctcttacc gcacacaggc tccgctgatt
      841 gactccttgc tcaatgaaat tggcgtttca ggcggttcac tggcggcatt gacttcaccc
      901 ttaatctcaa caactcccgt cgccgaaaac gtaaaataaa ttttcagcta tgaacgtccc
      961 tggcacaaaa agccggggat tttttatatc tgcgttccgc taaaaactgc aaatgatcag
     1021 gtcgttgcaa cgttttgcgt gaccgctcgg ggaaggcaaa actgcctctg ggaaagcgtt
     1081 gcgcgaggtc cggcgctcat caacaatcgg ggagtcagca aagggctgaa acgggaaagc
     1141 ccctcccgag gaaggggcct tacagaagga aagggttatg atgaagctcg tcatcatact
     1201 gattgtgttg ttactcgcaa gtttcgcagc ttattaacag ccaatcagag gggggagaaa
     1261 tcctccctca cccttgttcc tttactttag gttgaaaaaa aatcagcgtc aagaaggttt
     1321 cattaacaca tatccctggc ccaaaaagcc ggggattttt tatatctgcg ttccgctaaa
     1381 aggtgcaaat gctcaggacg ttgcagcgtt ttgcgtgacc gctcggggaa ggcaaaactg
     1441 cttcagggaa agcgttgcgc ggggtccggc gctcatcaac aatcggggag tcagcaaggg
     1501 gctgaaacgg gaaagcccct cccgaggaag gggccttata gaaggaaagg gttatgatga
     1561 agttcgtcat catactggtt gtgttgttac tgttaagttt cccgacttac taacaactga
     1621 tcagaggggg gagaaatcct cccttaccct tgttccttta ctttaggttg aaaaaacaac
     1681 agcgtcaata ggcctgcctg ctacgaagcg agatctgtga accgctttcc ggttagcctt
     1741 ttttatcctg ttggatcttc ttgatgatgt tagtggtcga gcaaccgtct tcgaagttga
     1801 gcaccagcac ttcgccgccg ttggcccaga cttctttact cccggcgatc tcttctggtt
     1861 tatagtcgcc gcccttcacc agtaaatctg gcaggatccc ggcgatcaag cgctgcggcg
     1921 tgtcttcttc aaacgacacc acccagtcga ccgcttccag tgcgcccagc acaatcatac
     1981 gctgttcgag cgggttaacg gggcgagaat cccctttcag ccgtttggtg gaggcatcgc
     2041 tgttcacagc gacaatcaag cggtcaccca gtttgcgggc atttgccaga tagaagacgt
     2101 gtccggcgtg caggatgtca aagacgccat tggtcatcac cactttttca ccgcgtttac
     2161 gcgctgccgc tacggccagc ttcagttcct cttcggtcat cacgccaaag ccggtatctg
     2221 cgcgcccacg cactgcattt tccagctcga tcggcgaaac cgtggaggtt cccagtttgc
     2281 cgaccaccac gccagccgcc gcattggcaa agaagcaggc ttcttccagc gaattacccg
     2341 ctgccagcgt tgccgccagg acgccaatca ccgtgtcgcc cgcaccggta acgtcataca
     2401 cttcctgcgc ctgggttggc atatgcagcg gcgctttacc cggttgcagc agcgacatac
     2461 cctgttcgga acgggtcact aacagagccg agagttcgta atcggcaatc agtttcatgc
     2521 cgcgctcaac aatctcttct tcggtcttac atttaccgac aacagcttca aattccgaga
     2581 gattcggtgt cagcagcgta gcgccgcggt agcgctcaaa atcggtacct tttggatcaa
     2641 tcagcaccgg aacgcccgct ttacgcgcca gttggatcat ctgctgtacg cttgccagcg
     2701 cacctttggc gtagtcagaa agcaccagcg cgccaatcga actcagcgcc tgattaatcc
     2761 gctcgtgcag cggctgcgga tcaacacctt cgaaaccttc ttcaaaatcc agacggatca
     2821 gctgttggtt gcgggaaagt acccgtaatt tggtgatggt cggatgcgtc ggtacagaaa
     2881 cgaagtcgca tttgacgttg acgtcggcca gagatttact cagcgcgcgc gctgcatcgt
     2941 caatgcccgt caacccgacc aggcgtgcat tagcaccgag agaagcgata ttcatcgcca
     3001 cgttagccgc gccgcccgga cgttcttcga tggtattcac tttaaccacg ggcaccggcg
     3061 cttccggcga gatacgactg gtggggccgt accagtaacg atccagcatc acatcaccaa
     3121 ccaccatcac tcctgcacgt tcaaactctg gcagcgttac tttcattcct gtctcctgag
     3181 agattcaaaa tttgcgcgcg ataataccat acttcattct tccaccagcc acttctgcca
     3241 gcttgcccgc accagatcac gctctgcggt gaagcaatcc tccgacacat ggcccggcaa
     3301 ttcctgtaat gccagatgat gaagttcatc gcgcagcgtc gtgtaagcac gggtcagcgc
     3361 catcgcttcc tgctcttcca taatgtcgtt ttgcgccagc agctccagaa tacgcacgtt
     3421 gtctgaccag cgcgttaact tcggtttttc atgagcgtag cgcaacacca gatattgggt
     3481 aataaattcg atatcggtaa ttccgccttc atcggctttg atatcaaagc gattacgatg
     3541 tttattgccg agatgagcgc gcattttctc gcgcatttcc cgcacttccg tttgcagagt
     3601 tttaccttct cgcggcagtg tcataatctc acgacgcacc gcgtcaaagt gcgcagtgag
     3661 ctgcggatcg ccgtacacta cacgcgcacg caccagcgcc tgatgttccc acgtccaggc
     3721 ctcgtttttc tgataatcgg caaatgcttc tgcggatgtc accagcattc ccgccgcccc
     3781 ggacggacgc agtcgagcat ccacttcata caaaatgccg gaagaggtac gcgtactgaa
     3841 cagatgcata atgcgttgcg ccagacgcag ataaaactgc cgcccgtcga tttcccgctc
     3901 gccgtcagtc atcgcatcca ttgggcaatc atggaggaag ataaggtcaa gatcggaact
     3961 gtagcctaac tcccagccgc ccagcttgcc gtagccgacc accgcaaaac cgcgcccttc
     4021 acgttcgttg aggtgatttg gcttaccgta gcgggccacc atttgacccc acgcctgctg
     4081 aacgacggca tctatcatag cttctgccag ccaggttaag tgatcgctca ctttcattac
     4141 cggtagcgta ccggcgatat ccgctgccgc aatgcgtaac agctgcgcct gtttgaactg
     4201 acgcagcgcc tcaagctgtt gttcttcgtc atcttccggc acgcgcagca aatactggcg
     4261 cagttcatca cggtaggcat cggtcgccgt cggctgataa agggtatttg gatcgagcaa
     4321 ttcatccagc aataatggat aacgcgctag ctggctggca atcatcggcg atgcggcaca
     4381 cagagaaatc aaatgtttaa gcgccgcagg gaattcactg agcaattcta aataggttgt
     4441 gcgggtaaca atccccacca gcaaggcggt aatgcgcgac agcgtgacag cagcgtcttc
     4501 acgcgcacag acatcactta gcagatgtgg catcagatgg tcgagcacct gacgtcctcg
     4561 cggcccgata gtacgcttat ccagctcttt gcggaaatca gcaatcagcg ttaggacctg
     4621 tttgcgatca tcctcgctaa gatgcgccag cactggcgta gtgtcatctt cctgcaacgc
     4681 atcctgccac agctcacgcc actgttccga cagcgactct tcctgagttt cactttcgtc
     4741 gtcgccaatc aattcattaa acacccggcg cacattggtc atatgtgcgg tcagcgcccc
     4801 ggtcagttgc ggccagtcag caaagtccat cgcccacgcc agccgcgcac ggttaagctc
     4861 atcagaagga agcgtttggg tttgttcgtc gttaatgctt tgcagcaggt tttccagacg
     4921 ccgcaggaac agatatgcca ctcgcaattg ttcagcatcg ttttcagaaa gcagatgcag
     4981 cgcggcaatg gcgctgagcg ttggcagtaa agagcgcgac tgcagcaacg gttcgcgccc
     5041 accgcgaatg agctggaaca cctgaacgat aaattcaatt tcgcgaatgc cgcctgcgcc
     5101 gagtttgata ttgtcggtca aaccacgtcg acgcacttca cgggcaatca tccctttcat
     5161 gttgcgcagc gactgaatca cgctgaaatc gatgtaacga cggaaaacaa acgggcgcag
     5221 catcgcacgc aactcgttag catagacgcc ttcgctatcg cccataatcc gcgctttgac
     5281 catcgcgtaa cgctcccagt cgcgtccctg ctcctggtaa taatcctcca acgcggcaaa
     5341 gctcagcacc agcgggccac tttcgccaaa cggacgcagt cgcatatcca cgcgatagac
     5401 gaagccatcc tgcgttggtt gatccagcac tttaatcagc cgctgcccca tgcgggtaaa
     5461 aaactgcgcg ttatccagtt cccggcgtcc gccctgcgta caaccatgtt ccggccaggc
     5521 aaaaatcaga tcgatatcag aggagaaatt cagttccccg ccgcccagct tacccatgcc
     5581 taagatcagc agcggttgcg cttcgccctg cgcattgcac ggcgttcccc actcgcggca
     5641 gcaggcgtca tacagccagt cacgcgccgc aacaatcagc gtctccgcca gataactgag
     5701 ctgctgcaat atgctctctt cagtaaccag tgccagcgtt tgcgcccatg cgatgcgcac
     5761 cataatgcgc cgccggaata gccgcagctc gcgcattaac ccggcttcgt cgctcacatt
     5821 actgagcgcc tcctgcaacc acacgctgta atgttgccat tcgtcagcct gtggcggttg
     5881 gctttccagt tccgtcagcc actctggatg cgcaatcacg ctgtcctgca caaaatcact
     5941 gaaagtaagt actgacttcg cctgtgcact aagtgattcc tcggctaaaa gctctggcag
     6001 ccgctcaaca acggtctgcc agtactgctg taacggtgaa gagagcggct tcataagcga
     6061 ttttatcctt gcctgattaa cgttttccac tgtgcaacca gaacggttcc tgattgtttg
     6121 cctcattacg gaaatgttca atttcaatgc gttgcccggt cgcaatagcg tgacgcagcc
     6181 cctgccagtt ctccagccag gcttgcgcga cgacaggatc atagtaaccc gccagcaaca
     6241 gtattgagtc aatatcacgc atcaggcgtg gcaactggtc acggtagcga tcgcctaacg
     6301 gctggcagaa aacgcttttc agttcagcgg catggcggga aagatggata tcggcaaagc
     6361 gtttgaagga gtcgctaatt ttgccctgcg ctttggcatc taaaaacggc tgccatgctt
     6421 tgcttaccag ccattcggtc aacgccagct tcgccattgc cgtttcggta gagtagaccg
     6481 ccgtcacggc agaaaccgca gaagcgatgg tcgcttcgca ttgagtcagc aaatcacgta
     6541 agtgagtact cgctttacgc ggcacaatgc caccgaacag catcagtgta tgacggacta
     6601 ggctaatggc tgccagcacc tgctctttcg ccgcatcgtt gccgcgtacc cacagttctt
     6661 catgatactg ccattgcgct aacaccagct cgagcgccgc ttccagcccc tgttccacat
     6721 cggcttttgc cgcaacatgc aaaatggtgg tcggtttgat ttcacgcgcc ggattgccct
     6781 gcgccagatg atagccacgc gccgctttgc tcaggctgcc ctggcgtaat ccggtttgcg
     6841 ataccagttg gttcgccagt ttcagcaccg cgcgcgtgtc gccgctaagc agctccagtt
     6901 ccagctcgca gataggctca gcaaattcac ccgctttcac ttccccctgg tcgagagcga
     6961 tttcaattcg gctgccattg atctctacta gccatttttc gcgataaaaa tcggtgctaa
     7021 acagcgactg cacgcgggaa gcgagatcgg caggcaattc gccgttcggc cagatttccg
     7081 tcggtaactg cgccaggtcg agcgtcggtt cgctcaacgc cacgttatat tccgggcgct
     7141 ggtgtaagcc gcctgtcact cttcctgcaa ctttcatggt catctcatag cgaccgtttt
     7201 cgccacgaat acgtaagccc atatcgtgcc tacgcagcca gttatccggc gtttcgtagt
     7261 aaatattcag caactgcacg gggtcatggt gctcgccgcc cagcgtattg agatggtcac
     7321 gcaacgcctc aacggcactg tgattaacaa taaactttaa ttcgatttcc tgagccatgg
     7381 cctttactta tcggttatgt cacataagtg acgatgaacg gcgaacttaa tgcgatcttt
     7441 tttgtcagta gatagtattt tgcgccaaat tgccatgcaa cgggcaattt gacgggcgta
     7501 aaagtttgaa gcagtggcaa ggcctgacac agaggattcc gattgatgtc gaatgctttg
     7561 cgtagcgaca ctgatgccac tactatcgtt ccactttcca tgacaataac gacagcctga
     7621 tgccaaaatt acgcctgatc ggattaactt tactcgcact tagcgcgact gccgtctcac
     7681 acgctgaaga aacgcgctat gtttctgacg aactgaatac ctgggtccgt agcggtccgg
     7741 gagatcatta tcgcctcgtg ggcacggtta acgccggcga ggaagtgacc ttattacaaa
     7801 ctgacgccaa caccaattat gcccaggtga aagacagctc tggccgtacc gcctggatcc
     7861 cgttgaaaca acttagcact gagccaagcc tgcgctcccg tgtgccagat ctggaaaatc
     7921 aggtcaaaac cctgaccgat aaactcacca atatcgataa cacctggaat cagcgcaccg
     7981 cagaaatgca gcaaaaagtg gcgcagagcg acagcgtgat caacgggtta aaagaagaaa
     8041 atcagaaact gaaaaacgag ctgattgtcg cgcagaaaaa ggtcgatgcc gccagcgtac
     8101 agctggatga caaacagcgc accatcatca tgcaatggtt tatgtatggc ggtggcgtgc
     8161 tggggcttgg cttgctgctc ggtctggtac tgccgcacct gatcccaagc cgcaaacgca
     8221 aagatcgctg gatgaactaa atcgccttct tcgttgcact gtcttattat taggaattgt
     8281 gtatttgacg aagggatgat ggcgtgaaga tttatctggt cggtggtgct gttcgggatg
     8341 cattgttagg actatcggtc aaagacagag attgggtggt ggtcggcagt acgccacagg
     8401 agatgctcga cgcgggctac cagcaggtag gccgcgattt tcctgtgttt ctgcatccgc
     8461 aaacgcatga agagtatgcg ctggcacgta ccgaacggaa atccggttcc ggttacaccg
     8521 gttttacttg ctatgccgca ccggatgtca cgctggaaga tgatcttaag cgtcgcgatc
     8581 tgaccattaa tgcgctggcc caggacgata acggcgagat tatcgacccg tacaacggtc
     8641 tgggcgatct gcaaaatcgt ctgttgcgcc atgtttcccc cgcttttggc gaagatccgt
     8701 tacgcgtact gcgcgtggcg cgttttgctg cgcgttatgc ccacctcggt tttcgtattg
     8761 ccgatgaaac tctgacgctg atgcgcgaga tgacccatgc gggtgaactg gaacacctga
     8821 cgcctgaacg ggtatggaaa gagacggaaa gcgccctcac cacccgcaat ccccaggtgt
     8881 tcttccaggt actgcgcgat tgcggcgcac ttcgcgtttt attcccggaa attgacgcac
     8941 tgtttggcgt tccggcacct gccaggtggc atccggaaat cgacacgggt attcatacct
     9001 taatgacgct ctcaatggcg gcgatgctga gtccgcaggt tgatgtccgt ttcgcaactt
     9061 tatgccacga tctcggtaaa ggactgacgc cgccagaact ttggccgcgt catcatgggc
     9121 atggcccggc gggtgttaag ttagtggaac aactatgcca gcgtctgcgc gtgccaaatg
     9181 aaattcgcga tttagccaga ctggtggctg agtttcacga tctcatccgc accttcccaa
     9241 tgctgaaccc aaaaaccatc gtcaaattat ttgattccat cgatgcctgg cgtaaaccgc
     9301 agcgtgtcga gcaactggcg ctgaccagcg aggctgacgt gcgcggcaga accggttttg
     9361 aatcagcgga ctacccgcaa ggccgttggt tgcgcgaagg ctgggaagtg gcgcagtcag
     9421 tgccgacaaa agcggtcgtt gaagcgggat ttaaaggcgt ggagattcgc gaggagctga
     9481 cccgacggcg gattgcggcg gtagccagct ggaaggaaca acgttgccca aagcctgact
     9541 gagtataaaa ctaaacgtta gcacgaaccg tcccctcgcc ccttcgggga gagggttagg
     9601 gtgaggggaa cagaccagca ccggtgcgaa caatcacccc caccccggcc ctctccctac
     9661 agagcgaggg agaaaacccg gcctgctccc gaaccttttc tcaatcaaaa gaacacgaca
     9721 tacaccgcag ccgccacaat aaagcgataa atggcgaacg ggataaacga aatgcgctta
     9781 atcaattgca ggaaggtttt aatcgctatc agcgccacca caaaagcggt gataaaccca
     9841 acggcaaaca tcgggatatc gccggttgtc aggaagcccc agcttttgta gagatcgagc
     9901 gccgttgcgc ccatcatcat cggtaccgcc agcaggaacg agaactcgga agcagcgtaa
     9961 cggctcacgc ccatcagcat cccacctgaa atggtcgccc cggaacggga gaaacccggc
    10021 cacagcgcca gacactggaa acagccaatc ataaacgcct gacgataggt catatcatca
    10081 agacccggcg cacgcggctc tttcggcttc aggcattcgg cggcaatcag caacaaaccg
    10141 ccaacgacca gcgcatacat cacatttatc gggttaaaca atgacttaat cgtgtcgtgg
    10201 aataatagcc ccagtaccac cgccggaatc atccccagca aaatgtggat cagcgttaaa
    10261 cgacctttgc tttcaccttc gtgctgcaac gggcggccaa agtggatgcc aatcaggcca
    10321 aacagacgcc gccagaacat cactactacc gccagaattg atcctaactg gatcacaact
    10381 tcaaaggttt tcgccgtgtc gccctcaaac cccaacaagt gaccgacaat aatcatatgg
    10441 cccgtgctgg ataccggcag aaattctgtc aatccttcga ccacacccaa tattgccgct
    10501 atcagcagcg agtgcatatc gctcatcaat aaacccctaa attattaaaa tgtaccgctt
    10561 gtccgaacta ctgcagtatg accaggttat aaccgtttgg tttaacagct gtaaaattaa
    10621 ttattttctt tcagattatt gccacgctca atgattacgc caacattcgc cgcccgcgcc
    10681 actgcgcctg gcttgctgag tttgatacgc acccacggcg agttgaagcg tgctaacagc
    10741 agctccgcca cctcttcagc cacgcgttcc accagcgcaa aacgcgcccc ctcgacgtgg
    10801 ctgaccaccg tttctgcaat gtcagcgtaa ctgaggcaat ccgccacatc atcacttttc
    10861 gccgctttac ggttatccca tgccatttcg atatcgaaca ctaacttctg ttcgatggtc
    10921 tgttcccagt cgtaaacacc aatagtggtg attaccgaaa gttgctctat aaatacaata
    10981 tccatcacgt cctgcctgct ttttggctaa ccggatacca cttccggcga aatgtgcgta
    11041 ttatccacag attcatcgtt gaacacgaat tttcaaaacg gaacagctta tgagtgcaat
    11101 cgcgcctgga atgatcctca tcgcgtacct ctgcggctcc atttccagtg ccattctggt
    11161 ttgccgcttg tgtgggctgc ccgatccgcg aaccagcggc tccggcaatc caggcgcaac
    11221 caatgtgtta cgtatcggtg gcaagggagc agccgtagca gtactgattt tcgacgttct
    11281 gaaaggaatg ttgcctgtct ggggcgcgta tgaattaggt gtcagcccct tctggctagg
    11341 cttaattgcc atcgccgcct gtcttggaca catctggccc gttttcttcg gttttaaagg
    11401 aggaaaaggc gttgctaccg cttttggagc aatcgcacca attagctggg atctcaccgg
    11461 agtaatggcg ggaacctggt tactgaccgt gctattgagc ggatactcgt cgctgggagc
    11521 gattgtcagt gcactgattg ctccgtttta tgtctggtgg tttaagccac aattcacctt
    11581 cccggtttcg atgctctctt gcctgatcct gctgcgtcat catgacaaca tccagcgtct
    11641 gtggcgtcgt caggagacaa aaatctggac gaaattcaaa agaaagcgcg aaaaggatcc
    11701 cgagtgattt ctggtggatc tacatgacct gatagccttc atcgggcttg cccagccgtt
    11761 gctggcacca tgccgccaga aattccgcgc agacacgaat ttcatgctgc gatagagcag
    11821 ctcccggtaa acagcccaga tattggcgct ttgtgcatac tccggcaata cttgcaccag
    11881 tttgccactc tccagaaacg gcaacacatc ccactcggaa cgcagcataa tccctttgcc
    11941 ctccagcgcc cattgcaaca ctatctcgcc gctattggag gaaagatgcc cgctaacctt
    12001 caccgatttt ttctcctgac cgttccccaa ctcccatatt ccgtgggtca tatcgcgctc
    12061 tttggtcacc agacagtcat gacgacttaa ttcctgtaat gattgtggct gcggatattt
    12121 ttgcagatac tcaggtgctg cgcataatat tcttttattt ttcgttaaca gatgcgcaat
    12181 ataataatca ggaatttcgt cattaatacg aatatccaga tcaatattat cctgcactaa
    12241 atcaatttgc cgatcgaaca attcaaaatg cacctgtaac tcaggataat tgcgcataag
    12301 ttcggtaatg gctggcgcaa tatggctgcg cccaaaacca aaactgcaac caatacgaat
    12361 catcccctcc gggcgcgttt tgatttgcgt gacgtcatcg accagtcgct gatattgggt
    12421 gaggatttcc agcgcatgtt cgtaacaacg ttggccactt tcagtcagcg ccacgccgcg
    12481 agcggagcgg ttcagcagcg ttgttgccag cgtattttca aggatttgga tccgcttggt
    12541 gacaaatgca ggcgtttgcc caagcgtcgc cgctgcagca ctaaaactcc cgctgtggac
    12601 gatttctacc agtacctgca agtctttggc taaaggccag ctattcagca tcgttaatta
    12661 tccgcagttg tgataagcgc agtgtatttc gcaaaacatt gccattccct gctctggcgc
    12721 aatattcatt cttattttcg cgttgattca cgaactgtta attacatatt cacggtggca
    12781 aaaaatataa aaccacattt ttagtgttag tttgtggcgg tgaatttttc cagacaaata
    12841 caaaaactgg agttgccatg atgagcgaaa gtaataagca acaggc
//
LOCUS       DAJVRP010000109        12695 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVRP010000109 DAJVRP010000000
VERSION     DAJVRP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.109"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OQN12_003338"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OQN12_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCS1655549.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OQN12_003339"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OQN12_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCS1655550.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OQN12_003340"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OQN12_003340"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCS1655551.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OQN12_003341"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OQN12_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCS1655552.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OQN12_003342"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OQN12_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCS1655553.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OQN12_003343"
     CDS             4025..4615
                     /locus_tag="OQN12_003343"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCS1655554.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OQN12_003344"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OQN12_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCS1655555.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OQN12_003345"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OQN12_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCS1655556.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OQN12_003346"
     CDS             complement(6551..6658)
                     /locus_tag="OQN12_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655557.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OQN12_003347"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OQN12_003347"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1655558.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OQN12_003348"
     CDS             7648..7875
                     /locus_tag="OQN12_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCS1655559.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OQN12_003349"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OQN12_003349"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCS1655560.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OQN12_003350"
     CDS             complement(9447..10703)
                     /locus_tag="OQN12_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCS1655561.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OQN12_003351"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OQN12_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1655562.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OQN12_003352"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OQN12_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1655563.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="OQN12_003353"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="OQN12_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCS1655564.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3034 a         3244 c         3197 g         3220 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcggtc tgctccagtc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatac gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 ggctctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcagtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAJVRP010000110        12622 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVRP010000110 DAJVRP010000000
VERSION     DAJVRP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.110"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..707)
                     /locus_tag="OQN12_003354"
     CDS             complement(<1..707)
                     /locus_tag="OQN12_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1655565.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNV"
     gene            complement(707..1387)
                     /locus_tag="OQN12_003355"
     CDS             complement(707..1387)
                     /locus_tag="OQN12_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCS1655566.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(1384..2583)
                     /locus_tag="OQN12_003356"
     CDS             complement(1384..2583)
                     /locus_tag="OQN12_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1655567.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(2584..2937)
                     /locus_tag="OQN12_003357"
     CDS             complement(2584..2937)
                     /locus_tag="OQN12_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655568.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(2937..3677)
                     /locus_tag="OQN12_003358"
     CDS             complement(2937..3677)
                     /locus_tag="OQN12_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCS1655569.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            3900..4190
                     /locus_tag="OQN12_003359"
     CDS             3900..4190
                     /locus_tag="OQN12_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HCS1655570.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(4214..4558)
                     /locus_tag="OQN12_003360"
     CDS             complement(4214..4558)
                     /locus_tag="OQN12_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655571.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(4571..5635)
                     /locus_tag="OQN12_003361"
     CDS             complement(4571..5635)
                     /locus_tag="OQN12_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655572.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(5638..5943)
                     /locus_tag="OQN12_003362"
     CDS             complement(5638..5943)
                     /locus_tag="OQN12_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655573.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(5945..6547)
                     /locus_tag="OQN12_003363"
     CDS             complement(5945..6547)
                     /locus_tag="OQN12_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655574.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(6547..8562)
                     /locus_tag="OQN12_003364"
     CDS             complement(6547..8562)
                     /locus_tag="OQN12_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCS1655575.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(8740..9165)
                     /locus_tag="OQN12_003365"
     CDS             complement(8740..9165)
                     /locus_tag="OQN12_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655576.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(9169..9609)
                     /locus_tag="OQN12_003366"
     CDS             complement(9169..9609)
                     /locus_tag="OQN12_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HCS1655577.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(9620..10765)
                     /locus_tag="OQN12_003367"
     CDS             complement(9620..10765)
                     /locus_tag="OQN12_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HCS1655578.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(10769..11326)
                     /locus_tag="OQN12_003368"
     CDS             complement(10769..11326)
                     /locus_tag="OQN12_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655579.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(11301..11690)
                     /locus_tag="OQN12_003369"
     CDS             complement(11301..11690)
                     /locus_tag="OQN12_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HCS1655580.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(11677..12231)
                     /locus_tag="OQN12_003370"
     CDS             complement(11677..12231)
                     /locus_tag="OQN12_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655581.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(12228..>12622)
                     /locus_tag="OQN12_003371"
     CDS             complement(12228..>12622)
                     /locus_tag="OQN12_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCS1655582.1"
                     /translation="SLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSENVTGK
                     DLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTSATLNP
                     DAGFWNNTRYGAEFYQLITMFGAGGRQL"
BASE COUNT         2897 a         3508 c         3160 g         3057 t
ORIGIN      
        1 tggacatttg tcgccgatac agtcggctgc cctgacgcgc cagtaacgtt caatgtaact
       61 gtgacagcaa ggactcttga gaatgtcatc gggaacctca tcccgtatgt tgaagataca
      121 ttcggggagc cattcaactg accgcactgt aaaattaatc cagacggaaa tttttgcgca
      181 gcagccgttg cggtattact tgacgcgaac aggctcatat caggtatctg attcgcccct
      241 gtacctacat tccttttagc cgcttctccc aaaccaaggt atgtgagaat gtcagcaata
      301 gtatttttcc caataatgtc acggccaaca gaagttaaat cagtctgcgc tgctgtatca
      361 tttccagtga aatatgggag tttatttgca cctgttgcga gcccagctaa tgccgacagc
      421 gtggcatcaa gcgcctggaa atctttcccg aaagcggtcc ccattttgga tataaacccg
      481 ttcaggtctc catcatcaag cacatccagc ctgcttttgt tggcggtgta ctgcgccaac
      541 gctgccgcga taaagctggc ttgccgaata gctttgttga cctgtgcgct ggatgcttta
      601 cctgccgtaa accctgaaag cagagccgga agtgcttccc agtcagcttg tgaggtgacg
      661 ttagcgttcg gatcaagcgc gaaaggttta aagttgttta ttgccattag agtattgtcc
      721 cccatgctcc aacatcgaac ccgccgatgt attcgttatc catatcaaac ccaaagaatt
      781 tagagccctc ggatggtgct tctaccgaag gcgtttcaac atcaccggcc catacgccag
      841 ctgctttaac ggtgagatag ccctgtttga tagcggcaat cagttcaaga gacacatcag
      901 aaatatcagt ctcggggaat acccaaaccg atatcgtcat gtcctggttg tcgacaattt
      961 gcatcctcag gcctgagcct tcagtcgcag cgtcaaggat gggaggcaga gagtcgttcc
     1021 gaccgtccca gttgttgata gcgattttcg ctttcagaat gatgcggtac gtctcatcgc
     1081 ttagcgtcgt atagccagaa tcaggatcat atggcccttt ccagatgccc tggtcatacc
     1141 caagcccgtc agtgtcccag ctgaaataaa ctccgctaat tggctggctg actatgcgac
     1201 tgcgtccgat ccacagaccg aggatgtcga gctgtacacc gacagcagta tcaatatcga
     1261 aggctgttat aagccctgac atagtgctgg acacatcaat cagcgggcgg gtgctcagat
     1321 ctatatggtc aaaaaagagt ggcttggtag cgtggtagtt agtgatcagt tcggtgtatt
     1381 tgctcatgag gtcaccgtga tactgatatt cgcggtgcta caggacgcag aagcatcata
     1441 ggcaatatca atgtttgatg ccgatacgct gccagacgac ttaccgatca gcaggtcggt
     1501 aatatcgtaa tagcgggcat tcccgccgct cacaacgccg aggtttgccg gggaataaat
     1561 acggctcagc agaacgtcgt cgccaattgt taggccattt atataatcgg caacagcctg
     1621 tttaatctgc tcgccgattt gagaggtata gccggtaaaa actttcaggg taatggctac
     1681 gaaaattggc acatcggtag agcgcgaaaa actgatgacg tgtggattac cgtaagtatc
     1741 cggcaccgtg acagaagttt taccgtaagt tgcggttccc tgccctttac tcccccggat
     1801 tgtttgggct atctcggtaa catcccctcc atcgacgatg gcggaaatag agtgtggcgg
     1861 cagcccgttg ctgtcggttg ccccagtgtc gttctcatat agtttgtgac gtgtcacgcc
     1921 agcaacatta gcgatagcac cgtcgacacc ttcaaacggt gtgatcgatg gtagcgcgac
     1981 gctttgcccc tgccgaatgc gcagctctgc gtcggtttcg gctggtgaac cgacagtagc
     2041 cgcaactgga ttggttaccg acacccaacc tcgggtcggg gtgttgatag tggtaatagt
     2101 cccggccagc gccgcaaccg aaccgctatt cgcacatgtg gccgtcacca gcacagtacc
     2161 atcaacgccg atcgctacac ttgcgggaaa attccagata atgccgtttt tatcccgtgc
     2221 ggagccattc gtgatagtcg tgcctgccgt accggttaac agaaggtcag cagtagagtt
     2281 tgtcgctact tttcgcgtga tcccgttaat tttcacattg ctgctaagcg ctgcggcctg
     2341 cgctgtcgtc ggtgaaaaag agttgtagat cccgatagcg gtgttgttag cgtcatgcac
     2401 ggcaagagcc accagcgcga ccatctgccc gtctttgctg tctggttcga ggtaggcatc
     2461 actaccgtaa atctgcctga aatagctggt cagtgtatct aggattgtct ggtaatcagg
     2521 cgcactaatc ccctgggcgg ttaccgttgc cgatagcccc agcgtgtcga ggttcaaagc
     2581 catttatgcc tcgcttgtta cagtcgtctg gccgtagatt gtgtcaatgg aggaagtgaa
     2641 ggtgacgcga cggctggtgc cgtcataatt ggtatcgaag gaaagaatcg acagaacgcc
     2701 cggtgtatcc tgtatgcgtt cgcgtatagc caggatgtag acatctgatc gctgcttccc
     2761 aagcactgac tgaacatacg gcgtgccttc cgtcagatcg agaaaccact gaccgcgcca
     2821 cagctcgaaa cgggttttta cggcctgggc gacacattcc ggactgtcga taaggaaagt
     2881 atcgtcaccc tggccgaaag tgtaatcgcc ttcagtatct tcgcgacggt atcgcattat
     2941 tgcggccctc cggtagttcc cccgccagtc tgtactccgc catgtttatg cgtggcgaca
     3001 ctgataccag aagctgttac gtcattcgtt accatcaccg gcccaagcat cgtcgcagta
     3061 ccgccgcttt cgcccattcc ctgagacaag ttgccattaa tcgttacgtt gccgttcagc
     3121 gtgatagtcg gggatgtgat tgtcgttcca ccttcagccg tagctgtaag ctggcccggt
     3181 gttttaatgg tgatgttatg tcctgcggcg acttctacaa acgccgcacc atcatcggtt
     3241 cgcagctgcg cggcgctggt actgataccg ctgattttcc gtgcttgcga ctgcgggcca
     3301 acgatggcga acgcatcaga taagtcatgc tggcgcgggt cgacggtctc ctgaacgccg
     3361 ccgctctgcc accaaaaatc gatgcaacgg tcagcaaaaa tcaggaggca ctcgtcgcct
     3421 tcttttaccg gaaaggtcag cgtgcaaccg ccgccgcgcg ggaagatgac cggcacatcc
     3481 accagcggtt ttaattcggt ggatccatcg ccaacaatac cgcgtagcgc cacctctact
     3541 gtgcaggtaa cagtctcagg atcgaacgac tgaatgatgc cgggcatcgc tacgcgcatc
     3601 tgggtagaca ccgaatcggc aatggcctgc gcggtctgct gctcaccgcc gatctgtgat
     3661 tgagttggaa ttggcataaa acccccataa aaaaaccagc cgaagctggt ttggtatcaa
     3721 ttatctctta gtaactaagc tacatcagtg tgaagtaagg cttttccgct gcaatggcac
     3781 acattcacaa ggtgtattag gatatgtttg caacaagtaa gcattgaaaa tcaatttcag
     3841 ccatgaagcc gccatagagt ggacctgcac catcgcatta ttgactacag gagtaacaaa
     3901 tgggatttag atttcgtaaa agaatccgga ttgcgcccgg actcgcgatc aacattagta
     3961 aaagcggagt aagcacttca attggtggta aaggttccac cattaacatc gggaaaaaag
     4021 gcgtaaagat gacaaatggc cttcccggca cggggctgtc gcataccact aacctttact
     4081 ctcccgggaa atcgacagaa aaaaaacaac ttactcataa gcaaaaaata attagaaaca
     4141 tcctgttcgt aattattgtg tttataataa ttaaagctca atatttttga cgcatgcccg
     4201 cctatctggc gggctatttt acttttcggc agtcgtatgt tgcatactga cgcggcgaat
     4261 ccatgctggc ttgcagccac tgtgcgttga gaatggcttt gccgtttcga ttgatgtact
     4321 caagaccaac ccatcttcca ggctgatcgg tagccatacg ccaatccatc ttgatattgt
     4381 tataatcgcc tttgtttttc aggaaggtga ttttttgcat ttctggcttt gcgccattga
     4441 ttctggctaa gccatcatct gcccaatgga ttttaaaatc accacattgc tgatccgcaa
     4501 aagcgggtgc tgatataagc accgcgaaca cggtaacagt gcaaagtaaa tgtctcacgg
     4561 ccccacctca ctaattcgtc gtcccttgca ttgctttcgg gctataaaga tcacgagcgc
     4621 cacgcgcaaa acacatcaaa tccatgtacc acgcctgacc tctggtgtcg ccagtatagt
     4681 cgatagcttt gacgatataa acgccatccg tcgcaatgct ggcagcctgt gacgtcgtgc
     4741 cggtcagcac acggttgccg ttctcttctg tttcggtgat acgcccgggc gactgtgcta
     4801 tttcgctatt gccgagcgtg gcgcggtaca ccgaagcctg atcgagctgg ataagaccat
     4861 taatacggat gtttgggttt atcaggcatc gcacgtttac tccgccaccc atcgtttgtt
     4921 gcggcatacc gatcaggcca gtattggcat tcaacacaat ggcttcgtga atatatttat
     4981 cctccggcac catctggacc tgaccatcca ccagttgcca tgtcgctttg cactgcgcag
     5041 caatattatc catcacgttg cggctggatg agtaaatcgc gcggccacga ggaaacacgg
     5101 tatcaggaaa atcgccggta atgccctgtg tcacgccgaa cgcgttgaaa tcctgcatcg
     5161 tcgcccggtg cagatccgca acggtatatc cagcggcaag cgtggtgatg gtggtcgcat
     5221 agaggaacgc ttcgtgatca ccaatggcct gaatcaacac ccaggaatcc gtaatgttgt
     5281 ccttcccggt gacggtgaag cgaatatcac cgtcaaaaat caggccgtag ttctgaccgt
     5341 tcacctgccc tatctggtct ggtgaaatct cccgggacac accaacctgg ctcgcatcaa
     5401 catccggcgc tataccgtca tacccggcaa tgatgcgaat tttggcaaac tcctgcccaa
     5461 gtatcttgtt cgtggtatcg gtcgaaaggt tgtaaatttt cacgttcgcc acgcgcggcc
     5521 agcgtgtgtc tgcccactcg atctggaacg tgaccttaaa gtcagacagg gaaacgccct
     5581 gcccgttctg gtccaacagt tgcagctcaa aatggcgcat ccagttaaga gacatttcta
     5641 ctcctgtacg aaaatgaggt ggctgtatgt gccgaggttg gttttggtgg gctcgtctgg
     5701 tgcgccctta tcggtcgcca ccaccagcgc gccatcaatg ccaagctgtg gatattgtcg
     5761 taaaaggttt acgccggtca gtagaggtac gccagagaga agcgcggcac cgccgctatc
     5821 catcacgtcc ataatccagc cagccgcatc acgccagatg atcctgagtg tatacgtggt
     5881 attgcccagc aaaactcgga actgctgatt gtcaggagaa agcggtattt cgttaaactg
     5941 catatcatcc cccgaatgct gatgtaacgc ttccgcccag ctggctcagc aaggattcgt
     6001 ttggcggtgt agtggatttc atcccggaat tctgcaccgc cgatgtgctg acgccatcct
     6061 gcatatctga tttatctgca acgctaacgc tctgcgtctg cgacatgatc acttcacgca
     6121 gggtaagcgt gcagttcagc acgttctcgc tggttttatc cgttgtcacc tcgatggctc
     6181 gcaccagcat attgctgtaa acccgctttc cggtcactac atcgaacggc accctagagg
     6241 actggagatc cagtagttgc tgataggtct ctttcgggct aagtccggcg ctgaggccga
     6301 ttgacgatgt atcaatgaag tccagcaacg aaccgccacc tgcgaagccg cattccatcg
     6361 tgacttcgct ggggcgctta tacgcatgat cggcgatgaa acccgacgcg ctattcgttg
     6421 ttggcttctc caccgggtgc tcagtaattt cgagcgcatc agaatgcttt tcagaaacaa
     6481 ccacgctggg gattaatatt ccaattcgcc gggattgctg gcgaaaaatt gctgataaaa
     6541 tatccattat ctcggtcctg cggggagttg ctgggttaac tgtgaattca cgcccttttg
     6601 acggtcaaca gtcaaacggg cagcctcgcg cggatcgaaa acgccgtgga tgttaatatt
     6661 cgtttcctgc tgaatcaccg gggcgctggt gggcatattg ctcattactt tcggaatgta
     6721 gttgcgcgtt tcctgcggca ttagccccat tccataacgc ttaacattcc cgatccccca
     6781 gttatatgat gccagtgctt tgctaaggtc tccgccgttc tgccgcaaca gctggctcag
     6841 gtacttagcg gctgcctgag ctgacttttc cgggtcgaat acatcattcc cacgcaggcc
     6901 catgtcgcgc gccgtgccat ccataaactg aaacagacct ttagcgccag cgcctgaaac
     6961 ggcgaactga ttaccgcccg attccgttat ggccacgctg cgcaataacc cctccggaag
     7021 ccggtagagc tgttccaggt tggtaaacat cggctgcatc catcccagca gttcagagcc
     7081 tgctttggtt ggctgtggcc gcttaactga ctggccgagc tgctcaggct catcatcacc
     7141 gaaccagccg cgcaccgttc ggcccacgct gcgaggatcg aatccccagt gctctttaat
     7201 ccagtcggcg gttccgttgg cgctgtctgt taccatcggc atcgctgacg gattttcgct
     7261 gccctgatta agcatctgtt tgccgatgct ggcggcatca gcccagcggc catctttaat
     7321 ggcgttgagc aggtcggcga tcatgttcag catcttgccg aactcaccca tttgcgaaat
     7381 gaagttgcta aaatcccact ttagggacca tgacttgggg tcaataccga gcaacttagc
     7441 cagcgctttt gtcaggtccg taacagtctt tttcaggtca ccaatcattt tgacggcctg
     7501 atcaatttca gtcttccatt tcccccagtc aatcaggctt ttgccacctt ctttccacgt
     7561 ctggtaatca tccaagagca gaccaagtgc aataataagc gtcgttatca tgcccaaggg
     7621 tgatgacatg aaagcggtat ttagcagccg ccatgcaacc aacagcccac tgaacaatgc
     7681 gataagctgc tgcgtggctg ggttcagctt tttgaaccag gcgatcacac cctcaacagc
     7741 ttgcccagtt cgccataaca cgcgggtaat cgcgtcacct gcccagagaa ttcctttgat
     7801 gatcttcgtg atgaccgctt caatcttcgg ccagttgtcg agtatctgcc tgcggaagtt
     7861 atcaatactg ccagcaaggc caccagctag gttagagcca atcttgtctt tcgcctgtcc
     7921 aagcgtcatc gtcagattac gcatggaggt catgaaaata ttggactgtt tagccgctga
     7981 ctcagcatta aaaccaatac gctttgccgt caacgcgtac tcagaactga gctgccccat
     8041 ccctctgcgc atcgccatca gcgtgttttc atcgatgccg agcatctgcg cgtattgctt
     8101 cgcgcggtaa tacggcatgt tgttgagctt ttggccaacg ccagtaaaga tggcagcagt
     8161 atcacgcatc tttccgctgg catcgcgggt ctggacacca agacggttca ggaacccttc
     8221 cgcccccgga ttgctacgca tgaagccagc caacccttcg agggaggaca tagccgactc
     8281 ggcgctggca ccggtttgcg atgcggcgta tcccagcgct ttgatgccct ggacactggc
     8341 ccccgtccgc tgggatgccc agtaaatttt atccagaccg ttcgcgatct gagtggtaaa
     8401 tccgacaatg ctcagcgctg cgcctttcac caccgcgccg accttcagaa cgttcgcggt
     8461 aacgcctttc agcacggctt caaacttatt agcgccagcc tgatcgatat cgaatcccag
     8521 cgaaacaagg aaatctttaa tcgtatctgc gttaccgctc attggccgct ctccatttat
     8581 ctacccgggc gtcgttatcc tcgcgcatgt cgaggtagtc attgagaagc gcgatgcggc
     8641 aaagatctac cgcaccgctg ttaaggtctt tctggtcaat atggaaggca agcgccgggc
     8701 gaagaataaa atcttcaccg cccggcaggc tgttgaaggt tattccgcta gcggggtggg
     8761 cgtctcgctg gtagggagtc cttgcaaaaa atttcccagc gagtcggcga ccacccgcgc
     8821 caccagttgc agcatggtaa gcaggtcgat atcgtcaaac gccatttcgc catgctggca
     8881 gaccggcacc cagcctttca tgtgctcgcg tgaaacaacg gaaagacagg ggaacaggat
     8941 agcgtccacg tcgccatcac tcagatcgga cacagcattg gcaatctttg gcaggatagt
     9001 agccatcgcg ccttcggtgt ctttgctgct gatcttctcc tgaacgctcc ggaagtccga
     9061 aaccatcccg gccagaaccg gcaacagctt gcgggacacc ttcagctgtt cgaaaacgct
     9121 gagctttgcg gtgcgatatt tcacgccttt aatttcgaat tccatgcgtt aaaactcccc
     9181 gagaagctgg tcaatcttgc cgcagtcgaa tacccaggcg acggttccgc cctctttagc
     9241 gttattgaaa tcaggctgtt tctggaatgc acacgaacgt gcagtagaaa tatcacccga
     9301 tgccgtgttg cgaatgacga tcacgttatt gccccaggtg gcagaggact ggctttgcgc
     9361 gttatacgcc agagacagct ttttattcac cggggaggtt ttgagtagcg tcaccgtaat
     9421 ggtgcctgac ttatcggcgt gcaggctgtg catcacctcg ccatcggcac cgatagtcat
     9481 ggtgtttttg ttgccgccca tggtctgggt gataccttcc tcagagttcg cagaaccctg
     9541 accaagatcg ataacgccgg tcggcccggt gagcgacgcg gttacatcga gaaaagaata
     9601 agttgccatt tatcgctcct tagcgaacca cgttgatctg cacatcggca taatgaactg
     9661 cgccagccag cttacaggcc acctggatta acggtgcttt gcgcgcttca cggtcggcct
     9721 gcgcctgttc ggacagcggt tgcgcataca cgtaatagcc tttggtcagc gtatcgccgg
     9781 aattcagctg tccgataggg ccaccattcc acacgccagc agctaccaga ccgttcgtga
     9841 cggactgatc catggactgt tcaacgttgg aaagcagacg ggtcacaccg gcatcagtct
     9901 gcggaatttt ggtggtgctg gtgtaaagca ggttatagag gttggtctga acgtagttct
     9961 gcaaccagtc gagcccgtgg cgctcgtcga agaagtcacc gttcgccatg acaccctgtt
    10021 gcaggatcgc cgtgtcgttg gcgtagtaca cgaatacatt gcagtttttt gcatcaagtg
    10081 ccgatgcctg gctgactgtc agtgtttcat acccgacacc cggctcctgc ttaaacttga
    10141 gcgtaatcgc ggtattactg ccattgaaat taaccgtgaa tgcccggcca aatgcagata
    10201 acgcagcgta tttattaccc gatgaatatt gaataaaact gcgtgaatat ccggcggttt
    10261 tcagttttga tgccaaatca tcgctggatg cagtctgcag gcatttctca tcgcttgtcg
    10321 taatcgccag aatacggctt acagaagagg attcgatcgc cgcagccact ttcagccagt
    10381 ctgcatccgg aatatcttca tcgtctgcaa tccccagccc ataccatgaa gtataatcga
    10441 gcatggcatt cacagcctgc tccagcgtct caggcgtggc ctgttcgctg tctcccttcg
    10501 ttttcaccca acgaccaaca aaaacctcct gaggtttcgg tgattgtgag aaaaacacct
    10561 gcgcagcctt atattctggt gattccacgc caaaatcttt tccaatatct tccgcggcag
    10621 aataacggcg aatgcgctca cttaccggaa tgattgtgga cgggccgaga atgagtaatg
    10681 caccaaaatt tcgccctgat gctgcacgcg gcgacatgat cacatcaaca ttaacaacgt
    10741 ttgatacagg caagccctgt gccatagctt aatctccgaa aaagatgact ggtgcttcca
    10801 ccagcgattt aataccgtac tcgcgcacaa ccttccggcg cagacgcacc gtcatatcgt
    10861 agcggcggac ccattgctga ttaataagtt cagggaagag acctgtgtaa tcgccaagag
    10921 acagccccag cgcattcagt gctgcgttgt tctgcggtac agatataccg tcacgaaacc
    10981 gggacgcata caccatcccc gccggaccat aaaacgaagc catacactca atcgtttcat
    11041 gccgccagag ctgagagcca tcatcggtct gtctggtgaa tgccggactg tcatcacctg
    11101 accatccgat aaccccaaac gcacaccagt tcgtttcaac cggtagcagt ggcggctgct
    11161 ctttctgcca gcgcgggcga accatcccgg cagacagacc ggaaacgtta cgcatccact
    11221 ggcttaacag cctgtcgagc gcttcgtcat aatccggatc gccactggtt ggtattaacc
    11281 atccgcgctc tgtactggtg ttattgctca accggagttc ccccatcaaa cggcatcaac
    11341 tcacaatgcg cctgaacgaa tccggcccca taagctgtat acgggtcgac gaaggtcaca
    11401 cgataatcac ggccctgata cgtcacgata tcggcatcac ggccagtctg tccctgcgtc
    11461 agtcgctcag tcgtcacaat cagaattgca ccgctgatta cctgccctgc ctgcatacgg
    11521 cggttttcca gagagcgatc aacagttacg actccggcaa actgcttttt aacttcactg
    11581 tcgctgccga tcccgtcctc atccaccgtt tgcactcggc gtgttaccca caaattgaag
    11641 tcgcaaaaat cggggtcaaa aagcacatct gttacatcaa gagtcggcat ctttatccct
    11701 cacaacatgg gtaatagctc tgcgatattg cccggtgtca attaatggtt tcgccagatc
    11761 ggttccggga gattcgccag caacacgccg agcaagttcc agtgttgccc ccttgcgccc
    11821 ccgacgagcc cgggcttcaa cggtgctatc agcaagcggt gtaaagccgg taatggtcat
    11881 gtaacgcctg acgccattcg cggccagcgt tccggcgcgg ttaagcgctc tttccgcacc
    11941 cgccgcattt ccatcaagcg cagcctgcgc cgctgctttg agctgcggca ccgtctgttc
    12001 ctctacggat ttaacgccgg ggatcaggtg cgggcgtggg ggtatgtttt gcgctggtga
    12061 gccgtattcg ttgacgtaac cgatcccggc attaccaaac ggaacatcct cacgctcgct
    12121 gtcttcttcc gggatgccca ccagcacatc ctttttggtt agcgaccgga gcgcatccag
    12181 aatggcctga gcgttatcaa ccctcgttgt tacaccactt ttgaaactca tagctggcga
    12241 ccgcccgcac cgaacatcgt gatcagctga taaaattcag cgccatatcg ggtgttattc
    12301 cagaagcctg cgtcagggtt tagcgtcgcg ctggtgtcat agctgacgct taccttgtca
    12361 acggacttgg aggactgaac accattggtt gaaccgcccg ggccgccaac cagcattgcc
    12421 cgactatctg ccgcccatag cgtcatgtag tgagccacga acaacccggc aaagtacgga
    12481 aacaaatctt tgccggtgac gttttcgctc agcagttcat cggccagatt cagacgaaac
    12541 tggatttgcg cttcgggata tttggcaggg tcagcaaact gcgggaagtc gcggcgaaaa
    12601 tcacttaccg ctggcaggct tt
//
LOCUS       DAJVRP010000111        12414 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVRP010000111 DAJVRP010000000
VERSION     DAJVRP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.111"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..1199
                     /gene="edd"
                     /locus_tag="OQN12_003372"
     CDS             <1..1199
                     /gene="edd"
                     /locus_tag="OQN12_003372"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1655583.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1236..1877
                     /gene="kdgA"
                     /locus_tag="OQN12_003373"
                     /gene_synonym="eda"
     CDS             1236..1877
                     /gene="kdgA"
                     /locus_tag="OQN12_003373"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1655584.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1933..3111)
                     /gene="purT"
                     /locus_tag="OQN12_003374"
     CDS             complement(1933..3111)
                     /gene="purT"
                     /locus_tag="OQN12_003374"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1655585.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3245..3535
                     /gene="yebG"
                     /locus_tag="OQN12_003375"
                     /pseudo
     CDS             3245..3535
                     /gene="yebG"
                     /locus_tag="OQN12_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3602..3958
                     /locus_tag="OQN12_003376"
     CDS             3602..3958
                     /locus_tag="OQN12_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1655586.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4285..4944
                     /locus_tag="OQN12_003377"
     CDS             4285..4944
                     /locus_tag="OQN12_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1655587.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5154..7214
                     /gene="ptrB"
                     /locus_tag="OQN12_003378"
     CDS             5154..7214
                     /gene="ptrB"
                     /locus_tag="OQN12_003378"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS1655588.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="OQN12_003379"
     CDS             complement(7211..7873)
                     /gene="exoX"
                     /locus_tag="OQN12_003379"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1655589.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7897..8553)
                     /locus_tag="OQN12_003380"
                     /pseudo
     CDS             complement(7897..8553)
                     /locus_tag="OQN12_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8655..8885)
                     /gene="holE"
                     /locus_tag="OQN12_003381"
     CDS             complement(8655..8885)
                     /gene="holE"
                     /locus_tag="OQN12_003381"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1655590.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9024..9398
                     /gene="yobA"
                     /locus_tag="OQN12_003382"
     CDS             9024..9398
                     /gene="yobA"
                     /locus_tag="OQN12_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1655591.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9402..10274
                     /gene="copD"
                     /locus_tag="OQN12_003383"
                     /pseudo
     CDS             9402..10274
                     /gene="copD"
                     /locus_tag="OQN12_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10287..10628
                     /locus_tag="OQN12_003384"
     CDS             10287..10628
                     /locus_tag="OQN12_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1655592.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11021..12097)
                     /locus_tag="OQN12_003385"
                     /pseudo
     CDS             complement(11021..12097)
                     /locus_tag="OQN12_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12063..12344)
                     /locus_tag="OQN12_003386"
     CDS             complement(12063..12344)
                     /locus_tag="OQN12_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCS1655593.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3079 a         3032 c         3137 g         3166 t
ORIGIN      
        1 tggccttact ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg
       61 gtactgccaa caccaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt
      121 cttttgttca tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg
      181 ttacacgcat gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga
      241 aagtggtggt gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca
      301 tgcacctggt ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg
      361 acctttctga tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta
      421 accacttcca ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagcaggcc
      481 tgctgcatga agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac
      541 catggctgaa taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca
      601 atgtgatcgc ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg
      661 gtaacctggg ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg
      721 aagcgccagc ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt
      781 tgctggaccg cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc
      841 cagaattaca taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg
      901 cgttagttac cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg
      961 taacaccaga agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc
     1021 gtgtgaatgg acagacaggc gaactgacac tgctggtaga cgaagcggaa ctggctgctc
     1081 gcgaaccgca cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca
     1141 gcgccttgcg tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag
     1201 acgacaaatt tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag
     1261 aatcaatcct gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg
     1321 cggtgccgat ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc
     1381 gtaccgagtg tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg
     1441 tgggtgccgg tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac
     1501 agttcgcaat tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta
     1561 ttcctctgat tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt
     1621 tgaaagagtt caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga
     1681 tcgcgggtcc gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact
     1741 accgtgacta cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg
     1801 cagatgcgct ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag
     1861 aaggcgctaa gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt
     1921 tttttgccgg gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat
     1981 ggcgtcaaca acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat
     2041 ttccggctta ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc
     2101 aaacgtgaca ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata
     2161 ctgacggatc ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga
     2221 gagatcttga gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact
     2281 gaaaatcacc tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag
     2341 tgccagcacc actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg
     2401 ctgtggttgc caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca
     2461 gaaatggacg ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac
     2521 gacgccttca acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca
     2581 tgcctgagca agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat
     2641 caccggtttt acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttt
     2701 gctatcggca aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg
     2761 gataccctcg cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc
     2821 ttcctcttca agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata
     2881 atgtggtttt tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac
     2941 atgggagcga tgcgcgacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac
     3001 ctctacgccg agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa
     3061 taacatcacg cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat
     3121 cccctgatta attccgctcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga
     3181 tgttgatttc tcaaccgaaa agaaatatac tgtataaaat ctcagttatt atgagaggct
     3241 gcaaatggcg gttgaagttt aatacgtagt cattcgtgag ggcgaagaga aaatgtcgtt
     3301 taccagcaaa aaggaagccg atgcttatga caaaatgctt gatacggcgg atctgcttga
     3361 cacctagctg acaaattctc cagtgcaaat ggaagacgag caacgtgaag ccctttcgct
     3421 atggctggca gaacaaaaag aggtgctgag caccattctg aaaaccggca aattaccgtc
     3481 cccacaggtc gtcggtgctg aatccgaaga ggaagatgcc tcacacgcgg cctgaagcaa
     3541 cttgcgctcc tgacaatttg taccatgctt tctcctgaat cattgtgcta tggagaaaaa
     3601 catgaaaaaa agaggggcgt ttttagggcg gttgttggtt tctgcctgcg catcagtttt
     3661 cgctgccaat aatgaaacca gcaagtcggt cactttccca aagtgtgaag gtctggatgc
     3721 tgccggaatt gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc
     3781 agatgatcaa aaaattgtcg gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat
     3841 tcagggtaaa gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat
     3901 tcattaccag gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttaatg
     3961 gcttctgata attcgattga ccgttggcgg atgcggcgcg agtgccttgt ccgtcccaga
     4021 acgcaccaca atcgtaggcc tgagaagacg caacagcgtc gcatcgggca atcagcatca
     4081 aacaatgcca ctcaaaacga caaactactt tcacctaaaa tacccttatc acttgatata
     4141 cagtaaatac cttacctggc gcgaatttct ttgaataacg gcctaccgcc ccgcttattt
     4201 gcgcatcttt tgtctcgata tacattttct gacatcctca tccgctatgc tacaggcact
     4261 aaaaatggtt attgaggagc aaatatggct aactggttaa atcaactgca atccctgctt
     4321 gggcaaagca gttcttctac ctcctcgtct gcggatcagg gattgggcaa actgttagtg
     4381 tcaggcgcat taggcggact ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt
     4441 cttacgaaat atggcaccaa cgcgttactg gttggcggcg gagcggtagc gggtacggtg
     4501 ctgtggaata aatacaaaga taaaattcgc gcggcgcatc aggacgaacc gcagtttggc
     4561 gcgcaaagta cgccgctgga tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc
     4621 gctaaaagtg atggtcatat tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt
     4681 gaagccggcg tggaagagca ggggcgtgta ctcattgagc aggcaatcga acaaccgctg
     4741 gatccacaac gcctggctac cggggtccgc aatgaagaag aggcgctgga aatctatttc
     4801 ctgagctgcg cggctattga tattgaccat tttatggaac gcagttacct gaatgcactg
     4861 ggtgacgcgc tgaaaatccc ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag
     4921 caaaaacgca ctttggcgga ataattccgc tctctggata gcctgaagtt gactatttcg
     4981 ggctatccag aaccgcacag ccatttgaac aggctgattt tctctgtctg cataacattc
     5041 gatatccgcg ccattacctg tcatcatcta agcaatgacg accctgtttc gcttgcatcc
     5101 ccggtgagtt ttgccaccct tataagatgt ttccaaccag aaagaacaat aacatgctac
     5161 caaaagccgc ccgcattccc cacgccatga cgcttcatgg cgatacgcgc atcgataatt
     5221 actactggct gcgggacgat acgcgttctc agccaaaagt cctggactac ctgcaacaag
     5281 aaaatagtta cggtcatcgg gtgatggcct cacaacaagc cttgcaggat cgcatcttaa
     5341 aggaaatcat cgaccgcatt ccgcaaagag aagtttctgc gccctacata aaaaatggct
     5401 accgctatcg gcatatttat gaaccaggct gtgaatatgc tatctaccag cgtcaatcgg
     5461 cattcagcga agagtgggat gagtgggaaa cattgctcga tgccaacaag cgcgcagctc
     5521 atagtgagtt ttattcgatg ggcggaatgg cgattacgcc cgataacacc attatggcgc
     5581 tggcagaaga ttttctttcc cgacgccagt acggcattcg ttttcgtaat ctggaaacag
     5641 gtaactggta cccggaactg ctggataacg ttgaacccag ctttgtctgg gcaaatgact
     5701 cctggacttt ctactatgtt cgcaagcatc cggtgacgct gctgccttat caggtctggc
     5761 gtcacgccat cggtacgcca gcatcgcaag atacactgat ctacgaagaa aaagacgata
     5821 cctattatgt cagcctgcat aaaacgacgt cgaagcacta tgtcgtcatt catctggcca
     5881 gcgccaccac cagtgaagtt cgcctgctgg acgcggaaat ggccgatgcc gagccgtttg
     5941 tttttctgcc gcgccgcaaa gatcacgaat acagccttga tcactaccag catcgttttt
     6001 atctgcgttc caatcgccac ggcaaaaact ttggcttata ccgtacccgt atgcgtgatg
     6061 agcaacagtg ggaagagtta attccgccac gcgaaaacat catgctggaa gggtttacgc
     6121 tgtttaccga ctggctggtg gttgaagagc gtcagcgcgg gttaaccagt ttgcgccaaa
     6181 ttaaccgcaa gacccgggaa gtcattggca ttgcctttga tgatccggcc tatgtgacct
     6241 ggattgccta caatccagaa cctgaaaccg cgcgattgcg ttatggttat tcttccatga
     6301 ccacaccaga cactttgttt gaactggata tggataccgg tgagcgtcgt gtattaaaac
     6361 aaacagaagt gcctggtttt gatgcggcga attaccgcag tgaacacctg tggatagtcg
     6421 cccgtgatgg cgtcgaagtt ccggtttcgc tggtctatca tcgcaaacat tttcgcaaag
     6481 gacacaaccc gttgctggtg tatggctatg gttcttacgg cgcaagtatt gatgccgatt
     6541 tcagttttag ccgcttgagt ttgttagatc gtggctttgt ctacgccatt gtccatgttc
     6601 gcggcggtgg tgagctgggg caacaatggt acgaagacgg taaatttctg aagaagaaaa
     6661 atacgtttaa tgattatctt gatgcctgcg atgcattgtt aaaactgggc tatggctctc
     6721 cttcgctctg ttatgcgatg ggcggaagtg cggggggcat gttgatgggc gttgcaatta
     6781 atcaacgccc ggaattattc cacggcgtta tcgcccaggt accgtttgtt gatgttgtaa
     6841 caacgatgct tgatgaatca attcctctta ccactggtga gtttgaagag tggggaaatc
     6901 cgcaggatcc gcaatattac gagtacatga aaagctacag cccgtatgac aacgtcaccg
     6961 cacaggctta tccgcattta ctggtaacga ccggtttaca cgattctcag gtgcaatatt
     7021 gggaaccggc aaaatgggtc gctaaattgc gcgagctgaa aaccgatgac catcttttat
     7081 tgctctgtac cgacatggac tcaggccatg gcggtaaatc tggccgcttt aaatcgtacg
     7141 aaggcgtagc gatggaatat gcttttctgg tcgcgctggc gcagggaaca ttacccgcta
     7201 cgcctgcgga ttaagtattt tccagataat gtttcagtgt taaacgcagc tccgggctca
     7261 tgctgttcag gttattaaat aaccagcgca gatagcccgg atagcgttcg gcaacgtcgg
     7321 aaaccgcttt gccacggtat ttgccaaagg tgaaggtcgt catcaacgac ggacgtccgg
     7381 tgatatcggc catctgttct gccgtccagc cggaggtgtt cataatatcg ataagcaacg
     7441 cggcggtgat ataacagtca tacaacgcgc ggtgatgatg caggcccggc ggtgtctgta
     7501 cattgagctt gcgtgtttta tataacgcca tattgctgta cttgagccct ggccacaaac
     7561 gacgggccag tttcatagtg caaatccact caccgggcat ctcaggcagt acgcggcggt
     7621 caaagctggc gttgtgcgcg acataccatt cactaccgta atagtgtggg atcacatctt
     7681 caatccacgg tttatcggcg accatggctt cggtgatgcg atgaatcgcc atcgcttgtg
     7741 gactaatagg acgatcgggg cgcaccagga ggctcatggg gttgacgatt tttccgtcaa
     7801 tgacatcaac agaggcaatc tcaacgatcc ctccctgcaa accgcaggtt tctgtatcga
     7861 taatgcgcaa catggcatgc tccaggccga aaaagcctag cgtaatggaa tgatatcgcc
     7921 ttgccaaccc tgtgatgaac gctgaccgac taacaataat gaaccacgat cggcgcgaac
     7981 gatgagacga ccatattcat cccatagtgc actgttacca cgggcatttg ccatcagcac
     8041 ggcaatcgag tactgatgtg aaaaaaattg taagcgacgg gccgacgcca gcaggtcggg
     8101 ttctcccaga cattgccctg tcgtaaacag tgaacaggtc gggtccatat ccataccttg
     8161 cggttgttca tcgaccaccg tgatggttct ggaacgacgg cccagacatg caccatgact
     8221 ctgatggtag ttcccaggcg tttttcgcca cggtgcgaac accgcaattc cacgaataaa
     8281 gcgatcgtta tattcaacgg gaaggccggc aatgatggtc attcgctagg tcgttgcggc
     8341 ataacaaagc gggtctaaca gtgaaagatc gggtggggca ggaagggcac gtcgcgaata
     8401 atcacacccc agtaaagaaa gcgatggaaa aactaacagc tgacattgtt gcctggcggc
     8461 ggccctcaca aactccagat gatgagctac ctgttcggtg agtgaggttt tacgaggctc
     8521 atattgcgct gctgcaactt tccagaacga cataatgact tccttttcaa aatgactgca
     8581 aattatgggg gcggtttaga aaatactcat aatttagcag gcgttatgta agaaagtgta
     8641 actctgtata agttttattt aagtttgggc tcgtaaggta aacgtgacag attgaccgaa
     8701 gccaaacggt gggcaataag ccgctcgcga aaccagctgc gcaaatgttc tggctgttca
     8761 cgttcaaccg cttcagcgat caccggcata ttgtagcgtt ctttaaaagc caccccggcc
     8821 gccgccaaat cgacattcac tttatccatt tctgtttgat ccagtttagc cagattcttc
     8881 agcattctta atctcctcat cattcgcggg caaaagttac taagagtgag cctggatggc
     8941 aagccggaat tgatgatcct cgactcagac attagacaag gttatgctct gtaaaaaagt
     9001 tattacaaaa aggaatagat aatatggctt caactgcacg ctcccttcgc tacgcgctgg
     9061 caatcctgac cacttcgttg gtgacacctt ccgtctgggc acatgcgcat ttaacgcatc
     9121 agtatcctgc ggcaaacgcg caagtgacag ctgccccgca ggcaatcact ttaaacttct
     9181 cggaaggcgt tgaaacagga ttcagtggtg caaaaatcac ggggccaaaa aacgaaaata
     9241 ttaaaacatt accagcgaag cggaatgagc aggaccaaaa gcaactgatt gttccactgg
     9301 ccgattcgct gaaacccgga acctacaccg ttgactggca tgttgtttcg gtggacgggc
     9361 ataaaacgaa aggacactac acctttagcg tgaaataaat catgctggcg tttacctgga
     9421 tcgcgctgcg atttatccat ttcacctcgt tgatgctggt ctttggcttt gcaatgtatg
     9481 gtgcctggct ggccccctta atgattcgtc gcttgctgac gaagcgttct ctgcgtttac
     9541 aacagcacgc tgccgtctgg agtttgatca gtgccactgc aatgctcgcc gttcagggcg
     9601 ggttgatggg aacgggatgg acagatgtat tttcgccaaa catttggcag gctgttttgc
     9661 aaacgcagtt tggtggcgtc tggttatggc aaattgttct cgccctcgta acgttgatcg
     9721 tcgcccttat gcaaccgcgg aatatgccac gtttgctgtt tatgctcact accgcgcaat
     9781 ttattctgct ggcgggagtg gggcatgcga cgctgaatga aggggtaaca gcgaaaatcc
     9841 atcagactaa tcacgcgatt cacctgattt gtgcggcggc gtggtttggg ggattactgc
     9901 cggtgctctg gtgtatgcaa ctcatcaaag gccgttggcg acactaggct attcaggcgc
     9961 tgatgcgttt ttcctggtgc gggcattttg cggtgatcgg cgtactggca agcggcgtgc
    10021 ttaatgcatt gttgattacc ggatttcccc ccacgcttac gacttactgg ggccaactct
    10081 tattgctcaa agccatcctg gtgatgatta tggtggtcat tgcgctggct aatcgttatg
    10141 ttcttgtacc gcgcatgagg caggatgaag atcgtgcggc accctggttt gtgtggatga
    10201 ctaaactcga atgggcgata ggtgcggtag tgctggtgat catcagcctg cttgcgactc
    10261 tcgaaccttt ttgatggatt acgaaaatga tgaaaaaaag tattctggcg tttctgttac
    10321 tcaccagttc tgcagcggcg ctggcggctc cacaggtgat aaccgtgagt cgttttgaag
    10381 tgggtaaaga caaatgggcg tttaatcgcg aagaggtaat gctgacttgc cgaccgggta
    10441 atgctttgta tgtcatcaac ccaagtaccc tcgtgcagta tcctttaaac gatatcgcac
    10501 aaaaggaagt tgccagtggg aagaccaatg cccaacccat ttcggtgatt cagattgatg
    10561 atcctaacaa tcccggcgaa aaaatgagtc tggcaccgtt tatagaacga gctgaaaaac
    10621 tctgttaatt acctaaaata gccttttgat ttccaataaa aaaaccgcct cagttctttc
    10681 accagaacgg gcggttttta acatttaagc tgatgagcac cgcgcttttt attgaccatt
    10741 ttgcacgcaa actggaaaac ctggcgtcgt catctattct taaagggcaa ggcaactaag
    10801 cctgcattaa tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg
    10861 aatacaggaa tcgtattcgg tcttttttta attgtattta aaatcaatta gttgcaaacg
    10921 tctccccgaa attccccgaa atttactcga atttctgtat tccggtcttt tttggttata
    10981 tcacaaccaa aatacattta acaatccatt tacgttaaaa tcagagcagt aagtacgttt
    11041 tttctctctc atcaagatac atttttgttg tcttctccga tgtgtggcca agtagacgct
    11101 gagcaaattc ttctccacat atttctttgt acaatcgtcc agccaggctt ctgatttcgt
    11161 gaaaagttgg tgggttttca ctgaacagga tacctgttaa ttttctggct gcgacaaatt
    11221 tctttgtcag gccgtccggg tgaatgctgc cgtcagggct gttttttcta atcccggcac
    11281 tgattagata atccccccta cttaccatgc ggcactgttc aactactgca ccaagtcgta
    11341 aaccagcgac aggaaggctg agtgacaggg ggatagcaat catcattcct gtcttaattt
    11401 gcctgatgtg gagacgatca tcataaatat cactgaaccg catattcgtt atgtcttcgc
    11461 gacgttgtcc tgttacaagg gctaagtcca tagctaatgg gaaccatgcc ggaagttgat
    11521 ctgctgcctc cctgatgcag ttgtatgtct ttagtttcag ccgttctctt gtaactacta
    11581 ttttcggtgc tcttgttggc gttactggat tttgagatat acgtccttca acaatggctt
    11641 cgcggaacat atcagataac acagaacgca ttgatcctgc catcgtgttt ttccctcctt
    11701 caatctacaa atcaagaaac tcggcgatat ggcgagtggt tatttctgtc agtaaaaccc
    11761 ctcctaattt ttcttttatt gtctccagtt gatttacccg agttttataa gtatttctgg
    11821 acgcttttct ccttataaga atcgttttgt aacgttcaat ccagcctgcc atagtaaatg
    11881 agtcgacccc tttaagcttt tcaattaggg cagcaggaga gtagtttttg tatatataat
    11941 gatttgcttc aattgcctgc gctactgcat ctcttcttga aattttacct agtgtaaatt
    12001 cttctttcgt cagagggttg cgccagtaat atgctttgtc tctccttcga tatgttaagt
    12061 ttctaggcaa attgggatcg tattttttcc gctgcatgtt ttaacttctc cattaacgga
    12121 ctgtctctcc cttgtcgccc attaggctga tggtgtgtta tatcggtatc aaccttattt
    12181 gggttgatat agaaagcctc cggaaccacc ctgtaactcc tcccgtgtag ttcaggtgca
    12241 ggataaatgt ttccattcct tgcccatcgt ctcagcgttg atattgatgg tgggttatcc
    12301 ggatatctga gttttcccca cgttttgagt gtcacaagat tcattgccat acctctcacg
    12361 atatgaccgc cagtaaatat acagaatact ggcgggtgtg gttgattttt aata
//
LOCUS       DAJVRP010000112        12221 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVRP010000112 DAJVRP010000000
VERSION     DAJVRP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.112"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..493)
                     /locus_tag="OQN12_003387"
     CDS             complement(<1..493)
                     /locus_tag="OQN12_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1655594.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OQN12_003388"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OQN12_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1655595.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OQN12_003389"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OQN12_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1655596.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="OQN12_003390"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="OQN12_003390"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1655597.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="OQN12_003391"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="OQN12_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1655598.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="OQN12_003392"
     CDS             3647..4156
                     /locus_tag="OQN12_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1655599.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OQN12_003393"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OQN12_003393"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1655600.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="OQN12_003394"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="OQN12_003394"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1655601.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="OQN12_003395"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="OQN12_003395"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1655602.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="OQN12_003396"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="OQN12_003396"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1655603.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="OQN12_003397"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="OQN12_003397"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1655604.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="OQN12_003398"
     CDS             complement(8798..9691)
                     /locus_tag="OQN12_003398"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1655605.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="OQN12_003399"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="OQN12_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="OQN12_003400"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="OQN12_003400"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCS1655606.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11581..>12221
                     /gene="allE"
                     /locus_tag="OQN12_003401"
     CDS             11581..>12221
                     /gene="allE"
                     /locus_tag="OQN12_003401"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCS1655607.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2970 a         3156 c         3117 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatgcaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 acacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catcgggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgcc
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgtta attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgcctgcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggagc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtaccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcagaaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata a
//
LOCUS       DAJVRP010000113        11923 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVRP010000113 DAJVRP010000000
VERSION     DAJVRP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.113"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..2338)
                     /locus_tag="OQN12_003402"
     CDS             complement(<1..2338)
                     /locus_tag="OQN12_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCS1655608.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNV"
     gene            complement(2272..2895)
                     /locus_tag="OQN12_003403"
     CDS             complement(2272..2895)
                     /locus_tag="OQN12_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCS1655609.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2892..4580)
                     /locus_tag="OQN12_003404"
                     /pseudo
     CDS             complement(2892..4580)
                     /locus_tag="OQN12_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="OQN12_003405"
     CDS             complement(4729..7356)
                     /gene="gyrA"
                     /locus_tag="OQN12_003405"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS1655610.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7503..8225
                     /locus_tag="OQN12_003406"
     CDS             7503..8225
                     /locus_tag="OQN12_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1655611.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="OQN12_003407"
     CDS             complement(8353..10284)
                     /gene="yfaL"
                     /locus_tag="OQN12_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCS1655612.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10297..11892)
                     /locus_tag="OQN12_003408"
     CDS             complement(10297..11892)
                     /locus_tag="OQN12_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCS1655613.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3308 c         2947 g         2911 t
ORIGIN      
        1 cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc cggatatcaa
       61 gctgaggaac ggcaactttg atcccggcgt tctgaaaact gtactgacca ttacgggtat
      121 acagcaccga aaaagtgatg ttaggcgcaa aggaattgct cactggtacc cgggcttcat
      181 acagggtatc gtttaaacgt tgtagcgtta gccagtttgc cggatgcgaa agcagcgact
      241 gctgttccac gcgatcgcgt tccagcgtca ataacgcttc atcaattggc tccgggaaag
      301 taatcagcat cttcgcggtt tcgcctggct ggtacagcgt tttatccgcc acgatatcta
      361 ccgtaccagt atgcgccgtg ctgcccttac cgctgacggc atgacttaac ccagcgagaa
      421 ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc aggtttagcg aaattgacgg
      481 taaaggattt gccgcctgac ggtagctctc cgctatggct cgtgcggtct tcgagacgca
      541 accattcata cgtaacagga acctgttttg aagattccag cgcggcataa cggaacacaa
      601 ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc gccaggccgc
      661 gttcaatgag gatctctttg gtggtggtga cgcgatacgc cgcgccgtca ctggcggaga
      721 cggttaacaa atagcggctc ggtttatcgg cggcggggag atttaacgcc acatgaccgc
      781 tggcgtcgga caccgtttcg ctgccttcca gcgacacggg gaaacgtccg gcataacgca
      841 aatcgttacc gaccattgat aattgctgag cgcgcaaact taactgcacg cgggcatttt
      901 ttaccggctc gccatccggg tagagcagtt gcagtttgcc gctgaccgct tcgccagttt
      961 tgaactcttt tttgtcgaga gctaaaccaa tctcgaaatg tggcttgatg tagtttgcca
     1021 cgcgaaaact gctgctatag acctgattgc ggtaagcaag acgtaactca taacctccgg
     1081 caacggcatt ttctggcagg cggaaacttc cctgcccgcc attgcgcgca tccagcgtga
     1141 catcgacggt ttgcaacaga ctgccgttag cgtccagcac cgaaagcttc gccggggcgc
     1201 tgacgatggg ggatgaatgc aacggatcgc ggaactcgcg gcccatcact ttaacatcga
     1261 cacgatcgcc tgcgcgatat agcgggcgat cggtaaaaat atacaagcgg gtgttgtaga
     1321 tttcgctttc gtagaagaag ttctcggaga caaaaacgcc gccttcagca tccttaccca
     1381 gaatgtatga acgttctggc gatatatgtt gtaactgcaa agtaccgctg tcatcggtca
     1441 ccccgcgggt catcacgcca agaccgtcag tccacaagat ctctgagccg ggcttcgctt
     1501 caccctgttt tttacccgcg gtccatacca gaagctcgtt acctgacact ttgctaagcg
     1561 ccacggtatc ggaaacaaac accaccgtcg tcgcccgata cccacctacc atcgcctcga
     1621 cgaggtacag ccccggctct tgttggccga gaggaatata aatattaccc ggctgcggcg
     1681 agatgaaatt gctggatgcg ccttccagtt ttaccccttg ctgcggctcg accggtttag
     1741 cctgccatag tggataacgg aactgttcca ccaagggata ttttttcagc ggggaaaact
     1801 ggttgttctg tacataacgg gaaggtttaa taatggcatt gccgagatgt aattcgggta
     1861 atgcctgagt cacattctgc cgtgactgag aagagaaagt acgctgcatc acgcggcgag
     1921 atttgccgta ccagttatcc cacagccagg tcagcgtatt gttcagcccg tcgcccagat
     1981 attgcggttg caccacaatg cgatgcaggt ttttctgctg gcgcaaaaat gccatcgggt
     2041 caggaatacg atataggcga acgtccacgc cgccgtactc ttccatctga tagcgccgat
     2101 aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc actgctgcta aaactgctgt
     2161 cagcaagcaa aaagaatgtt cccccggcgg gcggcgcata gttactggaa ggaagcgaat
     2221 catcagcttt agcaagccct gttcccacaa gagacaggga caagcacgct gctatcgcgc
     2281 cagaaaattt aaacgataaa tgccaatgaa attgggattg gaatcgttgg gtatccatcg
     2341 ggtgtccttc catgtcataa gttgttgcag actgactgcg cgcattccgt tgtcagtttt
     2401 cgtggcgctt ccggtgtggt agatgacgta acgccccatc cagaccatta agtgctgggc
     2461 atcgccctga tcgaaaaaaa tcatatcgcc aggcagcgcc tggtttatgt cttggccaat
     2521 aaactggctg ttgtactgaa tcaaattaat cgcggtcacg taggggccgt ttttcccgct
     2581 cccctgattc cagttttgcg ccagttgacg ctgttcaggt gttagcgtca tctctggcgg
     2641 caaatactgg ctagataaac cgttactttt tagccattta ctgtcgtgaa ctttcagcgt
     2701 ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc tgataccagc gtggacttgg
     2761 cccctggcgg agctgttctt gtgcaatgcg tacaaaccag gcgcgaaaca gcctggattg
     2821 ttcgacattc agcatttcgc tatgggcaac aacacaacac agccagcaaa tcagcgccag
     2881 cagcccgtgt ctcatagcgg ctgccaggtt atgggtagcc actgccaggc gacaccgggt
     2941 tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc cagcttcggc
     3001 attaataaag tttgtgcggc gttataaaaa accggttcga gattcttcgg cagactggtc
     3061 aacgtttcgt tacgcagcag tttcgccacg ccttgtggat tgatatagag cggaacgatg
     3121 ccatcagtgg gtattacatc caccattgcc gggcgggttt tattcagtgt ttgcagggcg
     3181 ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat cgccagcgac
     3241 acacggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc tttcggatac
     3301 tgaccgtatc gggaactcac ttcgcgacac cagatttgcg cttcgccctg ctgagtctgg
     3361 cttaccggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac
     3421 agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccgac aaacagcggg
     3481 gtttgcaatt ttgagtcttc ataccagcac agccccgcgg caccgtctaa cgccccattc
     3541 agcttgtcgt tttcctggct gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac
     3601 ggcaccgcca cacagaagct ggctccggca ggcatactgt tccagaccgg cgtaaaatcg
     3661 aaactggcat ctacgctggc ggattcatca tttaacgcca caaaactgtg ccagccgtcg
     3721 ttacccattt cgaagcgtac gccagcaaaa gaaggcatta accgctggta gccaaaaccc
     3781 agcaacctgg cgctgaccac gatgcgcagg cgtactggcg ttttttcggc tgtacgctct
     3841 tccaggccaa agctggcttg ccagcgtttt ttaccgctca acaaatcact tgcgatcgcc
     3901 gtggcttcgg tatcctgctg atcatcttta aacaacatat ccgtactgga aaacaccagc
     3961 atcttgtcct gatatgtcgc gaacatcagg gcgttattgc cgttatagcg caactgataa
     4021 acaggaatag tttcactatt tatcttgatg ctactgattt ccgttttgct taactagctg
     4081 tcgctggtag cggcaaacaa caatggctcc agcagtttgc ttaaaccact gcgctggatc
     4141 agcaccatgt aatgtgaaag atggccttgt ttatcgtgcc atagcgccgc ctgcgcgggc
     4201 tgatctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc atagacaatg
     4261 cggcgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata gaaaacgaaa
     4321 tcttcgctca gaacatcgtg gagaaacgga atggtgagga gatctttggg aagctggctc
     4381 agagagtcgc tgtctagaaa gaggtccggc tcattgagat cgatttgcag attgttgtgc
     4441 accaccagcg gcgacaacgt tttttctggc ccactgccag catattgcaa cgcccagacg
     4501 ccagcggaaa gcagtgctat tgcgccaaaa cctacaagac catagaaccg ccagcctttc
     4561 gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt ttttacgggc
     4621 acagccaaac tttaccgcgc cctaatacga caaaagccca gactttgcag cctggacttt
     4681 tcaattcaaa caagggagat agctcccttt tggcatgaag aagtaaaatt attcttcttc
     4741 tggctcgtcg tcaacgtcca cttccggagc aatttcatca tccccttccg cggcactgcc
     4801 gtcgatggta tccagatctt cctcgtcaac cggttcagca acacgctgca gacccactac
     4861 gttttcatct tccgcagtac ggatgaggat cacgccctgg gtgttacggc ccacgatgct
     4921 gatttctgaa acgcgagtac gtaccagcgt accggcatcg gtgatcatca tgatctggtc
     4981 gcagtcatct acctgcaccg cgccaaccac taaaccgtta cgttcggtaa ccttgatgga
     5041 gataacccct ttcgtcgcac gcgacttggt tgggtattcc gccactgcag tacgtttacc
     5101 gtaaccgttt tgcgttgcgg tgaggattgc gccatcgcca cgaggcacga tcagagagac
     5161 gactttatcg ccttcaccta agcgaatacc gcgcacacca gtggtattgc agcccatcgc
     5221 acggacagaa gactctttaa agcgcaccac tttaccttcg gcggagaaca gcattacttc
     5281 atcttcgcca ctggtcagat caacgccgat cagctcatcg ccgtcaacca gtttgatcgc
     5341 cactttaccg gcggtacgca gacggttgaa ttcggtgagg acggttttct tcacagtacc
     5401 gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac tcggtcaccg gcaggatcgc
     5461 ggtgatacgt tcgtcctgtt ccagcggtag caggttgacg atcggacgac cgcgcgcgcc
     5521 acgagtggct tccggcaact ggtaaacttt catcgaatag acgcgaccac ggctggagaa
     5581 gcacagaata tggtcgtgcg tgttcgccac cagcagtcgg tcgataaagt cttcttcttt
     5641 aatacgtgcg gcagatttac ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg
     5701 ctgatattta acgtagccct ggtgagagag cgtcacgacc acatcttcct gggtgatcag
     5761 atcttccagg ttgatgtctg cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa
     5821 ctgttcacga accagctcca gctcttcgcg gatcacttcc atcagacgat cggcgctacc
     5881 aagaatacgc aacagttccg cgatctgatc cagcagctct ttgtattcgt cgagcagttt
     5941 ttcgtgctca aggccggtca gtttctgcaa acgcagatcc agaatcgcct gagcttgctg
     6001 ttcggtcagg tagtacagac catcacgcac gccgaactca ggctccagcc attccggacg
     6061 cgcagcatcg tcgccagcac gttccaacat ggcggcaacg ttgcccagct gccacggatt
     6121 agcaaccagc gcagttttcg cttctgcagg cgtcggcgca tgacggatca gttcgatgat
     6181 cgggtcaatg ttcgccagcg ccacggctaa tgcttcaagg atatgagcac gatcgcgagc
     6241 tttacgcagt tcgaaaatag tacgacgggt caccacttca cggcggtgac gaacaaacgc
     6301 cgcgatgatg tctttcaggt tcatgatctt cggctgacca tggtgcaatg ccaccatgtt
     6361 gataccgaaa gaaacctgca actgggtctg ggagtagagg ttgttgagca caacttcacc
     6421 gaccgcatcg cgtttcactt caatcacgat gcgcataccg tctttgtcag actcgtcacg
     6481 cagcgcgctg atgccttcca cgcgtttttc ttttaccagt tccgcaatct tctcgatcag
     6541 gcgcgctttg tttacctgat acggaatttc gtggacgata atggtttcac gtccggtttt
     6601 ggcgtcaact tccacttcag cacgggcgcg gatatatacc ttgccgcgac cggtacggta
     6661 agcttcttca ataccgcgac gaccgttaat gattgccgcc gtcgggaagt ccggccccgg
     6721 gatgtgttcc atcagccctt caatgctgat gtcttcatca tcgatatacg ccagacaacc
     6781 gttgatgact tccgtcaggt tgtgcggcgg gatgttggtt gccataccta cggcgatacc
     6841 ggaagaaccg ttcaccagca ggttaggaat tttggttggc atgacgtcgg gaattttttc
     6901 cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc
     6961 atgggcaatt ttcgccagac ggatttccgt ataacgcatt gccgccgcag agtcgccgtc
     7021 gatggaaccg aagttaccct gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc
     7081 catacggacg atcgtgtcat aaaccgccga gtcaccatgg ggatggtatt taccgattac
     7141 gtcaccaacg acacgggcag attttttata ggctttgttc cagtcattgc ctagtacgtt
     7201 catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc
     7261 acggccaaca atgaccgaca tcgcataatc cagataggag ctctttagct cttcctcaat
     7321 gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa ccgctatccc tctactgtat
     7381 cccggattca aaggtcgcaa attataacac agccgcgcag tttgaggtaa acctatacgc
     7441 tttattcaca tccaatgcct gatatactcg tttgtcttgc caattacgga gtagaagtgc
     7501 caatgaatgc cgaaaaatcg ccggaaaacc ataacgtaga ccacgaagag atcgctaaat
     7561 ttgaagccgt cgcctcccgc tggtgggatc ttgaaggtga gttcaaaccg ttgcaccgca
     7621 ttaacccgct gcgtctgggc tatattgccg agcgtgctgg cggtttattt ggcaaaaagg
     7681 tgctcgatgt cggttgtggc ggcggcattc tggccgagag tatggcgcgc gaaggcgcga
     7741 cggtgaccgg tctggatatg ggctttgagc cattgcaggt ggcaaagctg cacgcgctgg
     7801 aaagcggcat tcaggtggat tacgtgcagg aaaccgtgga agagcacgcg gcaaaacatg
     7861 ccgggcagta tgatgtggtg acctgcatgg agatgctgga gcacgtaccc gatccgcagt
     7921 cagtggtcag agcctgtgcg caactggtga aaccaggcgg cgatgtcttt ttctcgacgc
     7981 ttaaccgcaa cggcaagtca tggctgatgg cggtggttgg cgcggaatat attttgcgca
     8041 tggtgcccaa aggtacgcat gatgtgaaga agtttattaa accggcagag ctgcttggct
     8101 gggtggatca gaccagttta aaagagcgac atataaccgg gctacattac aacccgatca
     8161 ctaattcctt taagctcggc cccggcgtgg atgtgaacta tatgctgcac acgcagaata
     8221 agtgaggttg atgtttggcc gcgccaatgc ctgatgcgac gcttgcagcg tcttatcagg
     8281 cctacaaatg ctctccgtag gccagataag gcgtttacgc cgcatccggc aattgtgatg
     8341 gtcagacagc gattaccatt tcaccgtcat cgacaaaaac cctgccgtct gggcaaaatc
     8401 atcactccct ttctgccacg ccacgctgcc gcgcagggac actcgctgac tgatattgcc
     8461 cgtgactccc acttttattt caccccgtag cttcaccgca tcgtcactga tagtgctgcc
     8521 atcttcttca atttccgtcg catggggatc gtgatagtaa ttcagatcta atgttggtgt
     8581 gacaccaacc gcggtacgcc attcgctgtg taaacccagc cgcgtctgaa tatcatcccc
     8641 ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa tcatcctgct gcacgccctg
     8701 ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc cccggtaacc actgataccc
     8761 gacttccagc gaggcgataa tccccgacga atggtaacga tccacgccat cttcatgttc
     8821 ggaaacatca ttgttaaacc acgcgtattg cagccagcta tccagccagg ccccctgctt
     8881 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgt ggttctggtt
     8941 atcggcgcga gttccggtca tattcgagcg gctgtcgccc tgattatcac tgtagccacc
     9001 aaccgcccca agcatccact cgccatccgt gccccagcgc ccgctaaaca agccgccgct
     9061 aagctgcacc gtagaagtgt cttcctgctg agccagttgc cctgccgccg tgtaatgata
     9121 acgtccgccg ataacacgta aattcagcgt ctgaccatcg ccaccagcgt gatcgcgtcg
     9181 ctccatcata aacgcctgat ttgccgcccg caggttattg agataaccgc caactttggc
     9241 attcagcacc ggctggtaag caggcgtagg ggccgggtcg ggggttgggt caggatccgg
     9301 cgtgggatca ggatcgggag tcgggtctgg ctcaggtggc gatggcggcg ttacttcttg
     9361 cgatcgcaga taccagtcgt tgttatcttc cactagcgtg tagtcatacg ctcccatgtt
     9421 gacgtagcca ctgccagcca gactgaactg tgcattgttt tgaaactgcg tgggatcagc
     9481 tgcgaaatca accactttaa tgcctgtcga tgtcggctca ccaatccctg taatggagtt
     9541 aaccaccaca gttgtgttgc cagcagtatt accgttcatc accaattgat cgcttaccga
     9601 gtcatcgccg tttaattcgc tgtcgaatag tagcgtaccg ccgccggtat aatcaccgtt
     9661 taccgtcagc gtatcgccag cgacgccatt ttgcaggcta accgcaccgg acgtattggt
     9721 cagatcacca ttaaccgtca cgtcagcaag ataggtcagg ctgtcagttt caaggttggt
     9781 attaagctcg cctgtcaacg tcacgccatc ggtagcattt accagcgtcc catcgccgtt
     9841 aaatagtgag gcattaaggg cgacggaagt atcctgcccg gtcagacgca aaaccgtacc
     9901 atcgctgatg tcgatagtgc cgctggaagt agcatcaatt gactgaatat cctgatccgc
     9961 gccagtaacg aacgttgccc catcaccaac ccacagtgac gaagcataag gaaagatatc
    10021 cgcaacatcg cctttcaggg tgccttcttc gacacgtacc gccgactgcg ttgtaccgct
    10081 ggcggtcagc tccagcgtac ccgcccccgt tttagtaaat gtgctaccct catcctgatg
    10141 ctgcccgctg ctgtcagcca tcagtgcgcc ccactgcgtg tctacccccg catcaactgc
    10201 cacttcaccg tcggcgcgca tttcaatatc acgcccatgc tcagtcgtcg tagagcgagt
    10261 atcgacattg gcagtaatct ccatcacgct ttcttgttac ttatcggtaa agataacctg
    10321 gcggttatag tgggtatctc caagctgcga gttgtcgctc accatcagcg taccggaggc
    10381 gatttgtgtt gtgcccaaat aactattatt gtttgccaga cttacctgcc cgtcgctcgc
    10441 atcgaccacc acgtagacgc catctttccc gtcgccgccg atatgcaaat tgtcgccacc
    10501 tgcaagatct accacatcct gactgccgat aaccgttccg ctgctgccac tgacttcaag
    10561 gccatcattg tatgatgtgc cgctctgcca agtggagaaa tcagagagat cgagcacacc
    10621 gccgtttaac acgatcgact gcggatcgtc ctggagagcg gtaagatctg ccgcgtcgcc
    10681 ttccagcgaa agcaccgcac catcatcaac gactatatcg ccggttagcg ccatcgactg
    10741 cgcccctgcc agcacgtagc tgccgttttg cgcaatggtg agctgaccag caccttcgat
    10801 gatgccagca aaactgccct gattaacagt aacgttgcca ccagcatcga tatttaaagt
    10861 gccattctga aagcccgtca atgcgtgcac aaaagtttgt tgggtcgagc caacgtttag
    10921 ctcagcctga ttctgatact gatcaatact ccctatcgtc agaccgtagc agtcttgcgg
    10981 atcgtcctgg caatgcgtat cgccgacatt catcagggag ttgctgcgac ccagggtaac
    11041 ttcaccgttt tcaatctgca tctcaccagt aaagtcattg ttatctgcat taagtaccag
    11101 atcgccggaa cctgttttgg tgattaaccc ggtaccagca atagagtcaa cagctccgtc
    11161 attctctgta ttgccgataa ccagcgtttt tccgtcggca atatcaaagg taacttcact
    11221 taagccgaga tacataaagc cacccgccgc agtggaggga ccatctccat agcctgctgc
    11281 gctattgttc tcatcgacta acacgccgcc gttctggctg tagctgtcat caacagaaat
    11341 atcaataaga tagggagccg tcgcgctatt ggtatatatc gccccgccat aaccttcggc
    11401 tgtgttattt gtaaaggcgg tgttatttat tatcgtataa cctgaaggat gcttgctgtc
    11461 gctattatta tcggtaacat cgattgcccc gccatcgcca tcactgtaac ttgttgatgt
    11521 atatgcctgg ttattgttaa aaacatcatc acttaaatag acgtcattat taatggtata
    11581 aattgcgcca cctttgccat cattagcgat attattgatg gcgttagtga cacgtaaatc
    11641 gacggcacca gtatcattag taccagaaga atagattgcg ccaccatagc cgcctgcgac
    11701 gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag caaatatggc
    11761 cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca ttccagtttc
    11821 attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc caccgctggc
    11881 attattggtc atatccgaaa aaaccagcca ttgcccatcg gcg
//
LOCUS       DAJVRP010000114        11706 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVRP010000114 DAJVRP010000000
VERSION     DAJVRP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.114"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            118..987
                     /locus_tag="OQN12_003409"
     CDS             118..987
                     /locus_tag="OQN12_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCS1655614.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1190..1543)
                     /locus_tag="OQN12_003410"
     CDS             complement(1190..1543)
                     /locus_tag="OQN12_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1655615.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1681..3327)
                     /gene="groL"
                     /locus_tag="OQN12_003411"
     CDS             complement(1681..3327)
                     /gene="groL"
                     /locus_tag="OQN12_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1655616.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3371..3664)
                     /locus_tag="OQN12_003412"
     CDS             complement(3371..3664)
                     /locus_tag="OQN12_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1655617.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3940..5196
                     /gene="yjeH"
                     /locus_tag="OQN12_003413"
     CDS             3940..5196
                     /gene="yjeH"
                     /locus_tag="OQN12_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1655618.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5212..5688)
                     /gene="fxsA"
                     /locus_tag="OQN12_003414"
     CDS             complement(5212..5688)
                     /gene="fxsA"
                     /locus_tag="OQN12_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1655619.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6025..7461
                     /gene="aspA"
                     /locus_tag="OQN12_003415"
     CDS             6025..7461
                     /gene="aspA"
                     /locus_tag="OQN12_003415"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1655620.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7579..8880
                     /gene="dcuA"
                     /locus_tag="OQN12_003416"
     CDS             7579..8880
                     /gene="dcuA"
                     /locus_tag="OQN12_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1655621.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8996..9334
                     /gene="cutA"
                     /locus_tag="OQN12_003417"
     CDS             8996..9334
                     /gene="cutA"
                     /locus_tag="OQN12_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1655622.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9310..11007
                     /gene="dsbD"
                     /locus_tag="OQN12_003418"
     CDS             9310..11007
                     /gene="dsbD"
                     /locus_tag="OQN12_003418"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1655623.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11044..11619
                     /locus_tag="OQN12_003419"
     CDS             11044..11619
                     /locus_tag="OQN12_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1655624.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2813 a         3022 c         2881 g         2990 t
ORIGIN      
        1 atatagagtg agcacggcta tttatgttat tttaacaact attactacca agcccattta
       61 tctggcttat aaattaaaat taattattta attgagaaat ggttagggag aacctacatg
      121 gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa tttcattgca
      181 ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttttttat gtctgtcatg
      241 gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca taaactggat
      301 gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa aatggcagaa
      361 aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg tcgggttaac
      421 acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac gctacatgac
      481 ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc gcgagcaatc
      541 attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac ctcactgtta
      601 gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga atatgacacc
      661 tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt gctttaccgc
      721 tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac gcgtgaagca
      781 acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag cccacgactg
      841 aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc gaataacgcc
      901 caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt acaatccacc
      961 agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc tggagccatc
     1021 cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt aaacgcctta
     1081 tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag gcctgataag
     1141 cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat cagttcgggc
     1201 acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac aacattccct
     1261 gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc tggttgcgca
     1321 gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg gaaagccagt
     1381 tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc tgctcgtcca
     1441 caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa cccgccatca
     1501 gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc cccataatca
     1561 atgagctgga cgccactcgc gtcatccgtg tctgaatctt atactagaaa gatgaccaaa
     1621 agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag tgcagggcaa
     1681 ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc ctaagtcagc
     1741 tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca ggccagccac
     1801 agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga tacccatgtc
     1861 gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt cgccgccttt
     1921 aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct gacgcagcgg
     1981 agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt tctgaccacg
     2041 caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag caaccacgcc
     2101 ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt ttttctcttt
     2161 catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg ccagtttcgc
     2221 tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa tctgctgacg
     2281 gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga tgatagtggt
     2341 ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg ttgctttttc
     2401 cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg cgatatcctg
     2461 cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa ctttcacgat
     2521 gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat cttcagcgat
     2581 gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca tttcgcggat
     2641 gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta ctgcgccagt
     2701 ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca taccttcaac
     2761 cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc cttctttacc
     2821 gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt tagcggagat
     2881 ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg ctttcagttc
     2941 ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg ggttcatgcc
     3001 cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg ttgcagtggt
     3061 ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca tctgcgcacc
     3121 catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac catctttggt
     3181 gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg gaccgagggt
     3241 aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac gagcgtcgtt
     3301 accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta tgttcagtgt
     3361 cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg atcaacactt
     3421 cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata acgatgtcgc
     3481 caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca ttgccgacag
     3541 ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg atgccgccag
     3601 cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat ggacgaatat
     3661 tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc ggagcttacg
     3721 tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc aagggggaaa
     3781 aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac ctcacatttc
     3841 atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc gaaaatctac
     3901 gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact caaacaagaa
     3961 ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac tggcgtgttt
     4021 gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc gtggcccgtt
     4081 ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg ccactatccc
     4141 agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct tgagcgagtc
     4201 accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact acaaattgcc
     4261 gccggattcg gccaagcaat gtttggctgg catagctggc aactgttgtt ggcagaactc
     4321 ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag tgctaatcta
     4381 caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg ggcgggcgat
     4441 atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac cgggttattt
     4501 gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc ccatcttgcc
     4561 tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg tctgctgctg
     4621 gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc ctatggtgaa
     4681 caaatggcgg cggcagcatc gcttccaaaa attgtagtac agttgttcgg tgtaggagcg
     4741 ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa catttatata
     4801 cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca ctacctggca
     4861 cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg ctgctgcgtg
     4921 gtgagcacgt tggtaattca tgccttagag atcaatctgg acgctcttat tatttatgcc
     4981 aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa attattgcaa
     5041 ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt actggcaatg
     5101 gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct gtttctgcca
     5161 aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac attaatcctg
     5221 gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat actcaccatc
     5281 aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc gagaaaagcg
     5341 caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata aaagcagaag
     5401 accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa taatcagcga
     5461 aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt gctgcatcag
     5521 cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg acgtgaatat
     5581 aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa agattgaaat
     5641 ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag gtttctcctg
     5701 taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcctt cagcttatat
     5761 tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt aatcccaaag
     5821 cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta aagtgatcca
     5881 gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga tgcaggggat
     5941 aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa gcaaatcatt
     6001 ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga agatctgttg
     6061 ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag agcgattgaa
     6121 aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg cggtatggta
     6181 atggttaaaa aagccgcagc catggcaaac aaagagctgc aaaccattcc taaaagtgta
     6241 gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg catggatcag
     6301 ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa caccaacgaa
     6361 gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata tcagtacctg
     6421 aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc gaccggtttc
     6481 cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca actgcgtgaa
     6541 ggctttgaac gtaaagctgt cgaattccag gacatcctga aaatgggtcg tacccagctg
     6601 caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat cctgctgaaa
     6661 gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa ccttggcgca
     6721 acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc agtgaaaaaa
     6781 ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga agcgacctct
     6841 gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt gaagatgtcc
     6901 aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct gaacgagatc
     6961 aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa cccggttgtt
     7021 ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac tgttaccatg
     7081 gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg ccaggccatg
     7141 ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa atgcattaac
     7201 ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat cggtatcgtt
     7261 acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa aatctgtgcc
     7321 gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac tgaagcggaa
     7381 cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc aaaacgctat
     7441 actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc acgtcagatg
     7501 acgtgccttt tttcttgtga gcagtaactt aaaaataaca atctaatatc aacttgttaa
     7561 aaaacaagga aggctaatat gctagttgta gagctcatca tagttttgct ggcgatcttc
     7621 ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg ggtgctggtt
     7681 cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc cattatcatg
     7741 gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta tctggttcat
     7801 cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc accgatcgtg
     7861 acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac actgccagtt
     7921 atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc tactgcagtg
     7981 gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt ttacatgtct
     8041 tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt catcccgtcc
     8101 accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa ctccaaactc
     8161 tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact gcgcggtgaa
     8221 aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct gctgggcgta
     8281 gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt tgaaaaaccg
     8341 ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac tctgaccacc
     8401 gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa agcaggtatg
     8461 agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt ttccaacaac
     8521 atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg gctgctggcc
     8581 gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc aaaagcactg
     8641 atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc tttcgctgcg
     8701 gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt acagatggat
     8761 gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt catcccgggt
     8821 actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt catgctgtaa
     8881 tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca tccttccccc
     8941 gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg tgtttatgct
     9001 tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac cggatgaagc
     9061 gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct gcgcgacctt
     9121 gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag aatacgaagt
     9181 gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat gcctgaagtc
     9241 tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg gagacacaga
     9301 ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag cacttccgtt
     9361 tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga tcaagccttt
     9421 gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat caaagacggt
     9481 tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat tgccgacgag
     9541 cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga gatttaccgc
     9601 gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac gttaactgtc
     9661 acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa aaccgttccg
     9721 ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc gcagcaagag
     9781 cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg tattggtatc
     9841 gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt gctgggtggt
     9901 aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt gcaggggatg
     9961 gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca gttccaggcg
    10021 gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt gctggcgatg
    10081 tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg tctcacgttg
    10141 atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg ggcgattgcc
    10201 ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct gtatatcgcc
    10261 caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt gggcatgggc
    10321 ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag cggcccgtgg
    10381 atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt cttcctgctg
    10441 gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg tgtcgcattc
    10501 tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg cgtggtgcaa
    10561 ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg ggcatttggt
    10621 gcgacgcata ccgcgcaaac tcaaacgcat ctcaacttta cacaaatcaa aacggtagat
    10681 gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga tctttatgcc
    10741 gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc gcaggtgcaa
    10801 aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga cgcacaagat
    10861 gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt ttttgacgga
    10921 caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga aaccttcagc
    10981 gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac ggaggaaaat
    11041 agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt acaagggatt
    11101 gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga cgagctacgc
    11161 cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct tagccaacag
    11221 atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga acaaaagctg
    11281 ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc gggctgtcta
    11341 tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca actggccgat
    11401 cagcaaaaaa gcgctgccta cgatttcacc cacgaactgt taaccacgct ggaagttgac
    11461 gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt aagccgaatg
    11521 ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga agatatcctg
    11581 cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag aaaagaagcg
    11641 atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga cgaaatgtgc
    11701 agatta
//
LOCUS       DAJVRP010000115        11659 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVRP010000115 DAJVRP010000000
VERSION     DAJVRP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.115"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            597..794
                     /locus_tag="OQN12_003420"
     CDS             597..794
                     /locus_tag="OQN12_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655625.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OQN12_003421"
     CDS             complement(744..1937)
                     /locus_tag="OQN12_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1655626.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OQN12_003422"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OQN12_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1655627.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OQN12_003423"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OQN12_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1655628.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OQN12_003424"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OQN12_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1655629.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OQN12_003425"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OQN12_003425"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1655630.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OQN12_003426"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OQN12_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1655631.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OQN12_003427"
     CDS             complement(10573..10716)
                     /locus_tag="OQN12_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655632.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11659
                     /locus_tag="OQN12_003428"
     CDS             10955..>11659
                     /locus_tag="OQN12_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1655633.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIRGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDS"
BASE COUNT         2957 a         2898 c         2992 g         2812 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaataaga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tgggactca
//
LOCUS       DAJVRP010000116        11631 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVRP010000116 DAJVRP010000000
VERSION     DAJVRP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.116"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(282..357)
                     /locus_tag="OQN12_003429"
     tRNA            complement(282..357)
                     /locus_tag="OQN12_003429"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="OQN12_003430"
     CDS             916..3168
                     /locus_tag="OQN12_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655634.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4481
                     /gene="csiD"
                     /locus_tag="OQN12_003431"
     CDS             3504..4481
                     /gene="csiD"
                     /locus_tag="OQN12_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS1655635.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4501..5769
                     /gene="lhgO"
                     /locus_tag="OQN12_003432"
     CDS             4501..5769
                     /gene="lhgO"
                     /locus_tag="OQN12_003432"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS1655636.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5792..7240
                     /gene="gabD"
                     /locus_tag="OQN12_003433"
                     /pseudo
     CDS             5792..7240
                     /gene="gabD"
                     /locus_tag="OQN12_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7248..8534
                     /gene="gabT"
                     /locus_tag="OQN12_003434"
     CDS             7248..8534
                     /gene="gabT"
                     /locus_tag="OQN12_003434"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCS1655637.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8772..10172
                     /gene="gabP"
                     /locus_tag="OQN12_003435"
     CDS             8772..10172
                     /gene="gabP"
                     /locus_tag="OQN12_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS1655638.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10193..10855
                     /gene="csiR"
                     /locus_tag="OQN12_003436"
     CDS             10193..10855
                     /gene="csiR"
                     /locus_tag="OQN12_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS1655639.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="OQN12_003437"
     CDS             complement(10856..11305)
                     /gene="kbp"
                     /locus_tag="OQN12_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1655640.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11389..11547)
                     /locus_tag="OQN12_003438"
     CDS             complement(11389..11547)
                     /locus_tag="OQN12_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS1655641.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2992 a         2855 c         2900 g         2884 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 ggggggagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg tccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gagcaaaaca tgcagggtgg aaattcgttg ctgctgcatc
     4081 tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt cgcccgatgc
     4141 gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca gtgttcgacg
     4201 ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag ccaaaagatt
     4261 ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc aaaggcattc
     4321 tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc tggctgcacg
     4381 gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt cagcgtggct
     4441 atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg aattgagcgg
     4501 atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc catgcaactg
     4561 attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg cccggcctgt
     4621 caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac gcccggcagc
     4681 ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg cgatcaaaac
     4741 ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct cgaaatggaa
     4801 cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga gtggataaac
     4861 gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat ttttgtgccg
     4921 tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat cttccaggcc
     4981 agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca taaaaacggc
     5041 gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat tagctgttcc
     5101 gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt tattatctgc
     5161 ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt taaccacctg
     5221 atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac ccgcatgatc
     5281 gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga aggctatcgc
     5341 aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg gattcgccgg
     5401 gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct gtgcaaaagc
     5461 ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga tctccagccc
     5521 tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat tgacgatttt
     5581 ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc ggcagcgaca
     5641 tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt ggcaagccag
     5701 agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca cgccgcattt
     5761 aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta acttattccg
     5821 ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag tcatcgacgt
     5881 caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg cagatgaaac
     5941 ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc tcaccgccaa
     6001 agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc aggacgattt
     6061 agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag gcgaaatcag
     6121 ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt atggctacac
     6181 cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga ttggcgtcac
     6241 cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag ccggtccggc
     6301 gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt tctctgcgct
     6361 ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta acgtggtcac
     6421 cggttcggca ggcgcggtcg gtaatgaact gaccagcaac ccgctggtac gcaaactgtc
     6481 gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga aagacatcaa
     6541 aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg atgccgatct
     6601 cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc aaacctgcgt
     6661 ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg aaaaattaca
     6721 gcaggcggtg agcaaactgc acatcggcga cgggctggag aaaggcgtca ccatcgggcc
     6781 gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg cgctggagaa
     6841 aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact tcttccagcc
     6901 aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga cgttcggccc
     6961 cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag ccaatgacac
     7021 cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct tccgcgtcgg
     7081 cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca atgaagtggc
     7141 cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt atggcatcga
     7201 agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg cgaaggaaca
     7261 gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt gggcaaattc
     7321 acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgttgaa ggccgtgagt
     7381 atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat ccgaaggtgg
     7441 tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag gtgctggctt
     7501 acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc gatttcgcca
     7561 agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta aaaatcgccc
     7621 gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac gggcgcacgc
     7681 attacaccct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg gggctgatgc
     7741 cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc gaggatgacg
     7801 ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat atcgccgcca
     7861 tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca gcctttatgc
     7921 agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat gaagtgcaga
     7981 gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg gcgccggatc
     8041 ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc accgggcgcg
     8101 cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc ggtaacccga
     8161 ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat ctgctgcaaa
     8221 aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc gaaaaacacc
     8281 cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg tttgaagatg
     8341 gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc gcccgcgata
     8401 agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc cttgtaccac
     8461 tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag tgttttgatg
     8521 aggcgaagca gtagcaccgc ccccatgcag gaggcgacgc tgcgcgtctt gtccggccta
     8581 cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac gtgaacagga
     8641 ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa tcaataacaa
     8701 ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga
     8761 gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg ctgaaatcac
     8821 gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca
     8881 gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc gccggattac
     8941 tggtggttat gattatgcgg atgctggcgg aaatggcagt tgctacgccc gataccggtt
     9001 cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc atcggctggt
     9061 tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc gccatgatcc
     9121 tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct
     9181 taactggcag taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc
     9241 tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt
     9301 tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc
     9361 ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg
     9421 cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat attgtccgcg
     9481 ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtcg
     9541 tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat cgctcggttc
     9601 tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata ttactttccg
     9661 taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac tccttaagcc
     9721 gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc ccgtacgtgg
     9781 cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat tacgcgcctg
     9841 cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg gtttatttag
     9901 tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc
     9961 gcttgcgaat gtggcttgat ccctggctca cctggctggt catcagcttt attacctttg
    10021 tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat
    10081 tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt
    10141 ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat
    10201 tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc
    10261 ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg ttgggccgtt
    10321 gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg
    10381 gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa
    10441 tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga
    10501 cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat
    10561 gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg
    10621 ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gttatcgatt
    10681 tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac acgatcagca
    10741 ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg agttaatgcg
    10801 ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact
    10861 cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt
    10921 tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg
    10981 cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg
    11041 ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt
    11101 tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt
    11161 gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct
    11221 tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt tctccggcat
    11281 ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag
    11341 tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt
    11401 ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt
    11461 aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg gcagaatgat
    11521 ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt
    11581 aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag t
//
LOCUS       DAJVRP010000117        10903 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVRP010000117 DAJVRP010000000
VERSION     DAJVRP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.117"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="OQN12_003439"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="OQN12_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS1655642.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="OQN12_003440"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="OQN12_003440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1655643.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="OQN12_003441"
     CDS             complement(2271..3686)
                     /locus_tag="OQN12_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1655644.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="OQN12_003442"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="OQN12_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1655645.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="OQN12_003443"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="OQN12_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10903)
                     /gene="mdtA"
                     /locus_tag="OQN12_003444"
     CDS             complement(9875..>10903)
                     /gene="mdtA"
                     /locus_tag="OQN12_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCS1655646.1"
                     /translation="RYLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAE
                     IDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSET
                     EGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHP
                     IDLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIK
                     VKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSK
                     HLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSRE
                     YAKKGARS"
BASE COUNT         2511 a         3070 c         3004 g         2318 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcaccaagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggtgaggta acg
//
LOCUS       DAJVRP010000118        10849 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVRP010000118 DAJVRP010000000
VERSION     DAJVRP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.118"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            77..427
                     /locus_tag="OQN12_003445"
     CDS             77..427
                     /locus_tag="OQN12_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCS1655647.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            480..875
                     /locus_tag="OQN12_003446"
     CDS             480..875
                     /locus_tag="OQN12_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS1655648.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            916..1659
                     /gene="cmoA"
                     /locus_tag="OQN12_003447"
     CDS             916..1659
                     /gene="cmoA"
                     /locus_tag="OQN12_003447"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1655649.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1656..2627
                     /gene="cmoB"
                     /locus_tag="OQN12_003448"
     CDS             1656..2627
                     /gene="cmoB"
                     /locus_tag="OQN12_003448"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1655650.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2792..5221)
                     /gene="torZ"
                     /locus_tag="OQN12_003449"
     CDS             complement(2792..5221)
                     /gene="torZ"
                     /locus_tag="OQN12_003449"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCS1655651.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5246..6346)
                     /locus_tag="OQN12_003450"
     CDS             complement(5246..6346)
                     /locus_tag="OQN12_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1655652.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6734..7480)
                     /gene="cutC"
                     /locus_tag="OQN12_003451"
     CDS             complement(6734..7480)
                     /gene="cutC"
                     /locus_tag="OQN12_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1655653.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7494..8060)
                     /locus_tag="OQN12_003452"
     CDS             complement(7494..8060)
                     /locus_tag="OQN12_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1655654.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8276..10009
                     /gene="argS"
                     /locus_tag="OQN12_003453"
     CDS             8276..10009
                     /gene="argS"
                     /locus_tag="OQN12_003453"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1655655.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10186..10674
                     /locus_tag="OQN12_003454"
     CDS             10186..10674
                     /locus_tag="OQN12_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCS1655656.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2649 a         2769 c         2594 g         2837 t
ORIGIN      
        1 aatcttacat ttctgtctat cataaccact agcatcacct ttaggaacca tctgaaacgg
       61 gaggtgtatt tagaccttgc taatcttaga cagccgcccg gttcatgcat cacgtccaca
      121 cagtgaagct attcgcgacg ctcaacgaaa aaaacctaaa gttccggtac atgctgtact
      181 gacggcgaca aatccgctga tccgttttat cggtagtgat gatatgacgc aaaaccggga
      241 attatttcag gtctggttac aaaaattagc gcagtggcat cagaccacta cgccttatct
      301 ttttttacat acgccagata ttgcccaggc cccggaactg gtacataccc tgtgggaaga
      361 cttacgtaaa acgcttccag agatcggagc agttccggct attccacagc aatcttctct
      421 tttctgaatt tgccacctat catagacagg tgccatcggc cattttaaag ggagtttgta
      481 tggtaagcgc gctatatgcc gttttaagtg cgttgttatt aatgaagttc tcttttgatg
      541 tcgttcgcct gcgaatgcag taccgcgttg cctatggtga cggtggtttt agcgaactgc
      601 aaagcgctat tcgcattcat ggtaacgcgg tggaatatat tcccatcgcg attgtgctga
      661 tgctgtttat ggaaatgaat ggcgcagaaa cctggatggt gcatatttgc ggcatcgttt
      721 tgcttgctgg tcgtctgatg cattattacg gttttcatca ccgtctgttt cgctggcgac
      781 gttccggcat gagcgccacc tggtgtgcgc tgttgctgat ggtgctggcg aatctttggt
      841 atatgccctg ggagttggtt ttctccctgc gttagcgcac aatacgccac tttctttttc
      901 ccggattttt acgttatgtc tcaccgcgac acgctatttt ctgcccctat cgccagactg
      961 ggcgactgga cctttgatga acgggtcgct gaagtcttcc cggatatgat ccagcgttcc
     1021 gttcccggct attccaatat tatttccatg attggtatgt tagccgagcg cttcgttcaa
     1081 cctggtacgc aggtttacga tctgggttgt tctctgggcg cggcgacgct ctcggtgcgt
     1141 cgcaacattc atcatgataa ttgcaaaatt attgccatcg acaactcccc ggcgatgatt
     1201 gaacgctgcc gtcgtcatat tgacgcctat aaagccccta cgccagtaga cgtcattgaa
     1261 ggtgatattc gcgatatcgc cattgaaaac gcatcgatgg tggtgctgaa ttttaccctg
     1321 caattcctgg aaccttccga gcgccaggca ttactggata aaatttatca aggactgaac
     1381 ccgggcggcg cgctggtgct ttcggaaaaa ttcagtttcg aagatgccaa agttggtgaa
     1441 ctgttgttca acatgcacca cgactttaaa cgtgccaacg gttacagcga actggagatc
     1501 agccagaagc gcagcatgct ggaaaacgtg atgctgaccg attccgtgga aacccataaa
     1561 gcacgcctgc ataaagccgg ttttgagcat agcgagctgt ggttccagtg ctttaacttt
     1621 ggttcactgg tggcattaaa agcagaggac gctgcatgat cgactttggt aacttttatt
     1681 ctctgattgc caaaaatcat ctttcacact ggctcgaaac gctgcccgcg cagattgcta
     1741 actggcagcg cgagcagcag cacgggctgt ttaagcagtg gtccaacacg gtggaatttc
     1801 tgcctgaaat taaaccgtat cgtctggatt tattgcatag cgtaaccgcc gaaagcgaag
     1861 agccactgag caccgggcaa attaaacgca ttgaaacgct tatgcggaac ctgatgccat
     1921 ggcgcaaagg gccgttctca ctgtatggcg tcaacatcga taccgaatgg cgttccgact
     1981 ggaaatggga tcgtgttctg ccccatcttt ctgatttaac cggacgcacc attctggatg
     2041 tcggctgcgg cagcggttat cacatgtggc gcatgattgg cgcaggcgcg cacctcgcgg
     2101 tggggatcga ccctacgcaa ctgtttctgt gccagtttga agcggtgcgt aaactactgg
     2161 gtaacgatca gcgcgcacat ttgttaccgt taggtattga acagcttccg gcactgaaag
     2221 cctttgatac cgtcttttcg atgggcgtgc tttaccatcg ccgttcgccg ctggagcatc
     2281 tctggcagtt gaaagatcaa ctggtgaatg agggtgaact ggtgctggaa acgctggtta
     2341 ttgatggcga tgagaacacg gtgctggtac caggcgatcg ttacgcgcaa atgcgtaatg
     2401 tctatttcat cccttccgcg ctggcgctga aaaactggct gaagaagtgt ggttttgtag
     2461 atatccgcat tgtggatgtg tgcgttacca ctactgaaga gcagcgacgc actgaatgga
     2521 tggttaccga atctctgtcc gatttcctcg acccacatga tccgagtaag acagttgaag
     2581 gttatcctgc gcctaaacgc gcggtgctga ttgcgcgcaa gccgtaaagg tctggtatta
     2641 ctgccggatg cggcgtgaac gccttatccg gcctacaaac ccttgctaat tcaatctatt
     2701 gcagggacta tgtaggcctg ataagcatag cgcatcaggc agttttacgt ttgcataacc
     2761 tcagcgcccg tttccgggcg ctattcactt cttacgcctg aacagcaggc ttatcaaatg
     2821 ccgttaactt cggtgcgtta cccgtatatt tttcgatata caccagcgca gagttaccgg
     2881 cacaggcatt tgcccgctgc gagctgggga tatccgccgt taacacgttc gcactgccgt
     2941 ttttacacaa gccgttttcc agatctggcc atgcaccttc atgcacgcat accacgcctt
     3001 ttttgatccc gtcagtcacc accgcgcctg tcaaaatctg accacgtttg ttccagacac
     3061 gcaccagatc gccattcgca ataccaaatc gagcagcatc ttcggtgtga atagtgatcg
     3121 gttcacgatc ggcaaccgca tattttttac gcagttccgc atagttaagc tgactgtgta
     3181 aacggtgtgc cggatgtgcg gtcagaagct gcaactgctt ctcgtcggcg gtacccttcc
     3241 actcatcagg cgcaagccag gttgggtgtg ccgggcaatc cttatagcca aatttttcca
     3301 gcgttttgga gtaaatctca attttgccgc ttggcgtacc cagcgcattt ttcaccggat
     3361 cggcgcggaa atcaccataa cgaacgtact gttcgttctt ctcgctgcgg cgcatttcga
     3421 tcagtttatt ttgctgccag aaggcattaa acattggcat agtgacgcgt tgcgcacggg
     3481 cccctttctg agcggcatca tagaaaaatt tcagccacgc catttcatct ttaccttcgg
     3541 tatagatctc ttttccgcca ggtttgagta attccgcaag atcggcaaac acgtcaaaat
     3601 cgttacgcgc ttcaaattgc ggagcaacag cctgcttcat cggcacaata tgctggttgc
     3661 tgtaatcacc ggtcatcgtc aagtcattgc gctcaaacga tgtggtgatc ggtaatacga
     3721 tatctgcgtg tttggctgct gcggtccagt agcattcaga aacgacgatc atctccggtt
     3781 tctgccaggc tttgatcaga cgattagtat cctggtgatg ggtaaagtta ccgccacccg
     3841 cccaccagat cattttgata ttcggatacg tttgttcttt accgttatgc tgatatttgc
     3901 cgccaggatt ttccagcgca tcgacgatac gggcaacagg aatcgccgtc attccgccat
     3961 catcagcggc ctcgctggcc tgtccggcaa tcgccgcaga catttccggc agcacgccgc
     4021 caacacgcgt cgggttgcca ccattggagt aatgataaga gaaaccaaaa ccgccgcccg
     4081 gtgtaccaat ctgccctaac atcgccgcca gcgtcaccag catccagtgt ttctgttcac
     4141 catattgctg gcgctgaatt ccccagcctg ccatcagcat tgtgcgattc gccgccatca
     4201 gttcagcgag tttgactatc tgcgcttccg gtacgccagt aatttccgcc gtccagaccg
     4261 cgctcttcgg cgtattatcg ctcttacctg tcagatactc ttcaaactgc ggatacccgg
     4321 tagtgtattt ttcaaggaac actttatcgt gtttgccttg tgtcatcagg gtatgcgcaa
     4381 tccctaacat cagtgccacg tcggtgccca tattcggcgc gatccaggtg gcattatcgc
     4441 caaagaattc gatggtttcg gagcggatag gatcaatggc aatcactggt ttgccagatt
     4501 ttttcagctg atggaagtat tccagccctt gctcatcggt actgctccag gcaattttta
     4561 aggtattcag cgggttcatt ccccacagca ccacaacctg gctgttttcc agaatcagcg
     4621 gccaggaggc ctgctgttca tacacctcta cagaaccgac cacatgcggc atgatcacct
     4681 gtgctgcccc ggttgaataa tcgccgctat gcccggaata accgcccgcc aggttcatat
     4741 aacgttgcag taaggtttgc gctttatgca acacgccaga agagcgccag ccgtaagatc
     4801 cggcaaaaat ggccgatggt ccgttagctt tacgaatacg ctcatgttgc tcatgaatca
     4861 gttttaatgc ctgttcccag ctcacctgta cataggtatc ttcgccacga cctttcgccg
     4921 gttgcagtgg attgtcgaga tagctttttc tcaccatcgg atgctgaata cgcgccgtgg
     4981 tgtgtacctg atccgccgcc gtagactgta aggagttcgg tatggttttc gccagcgcgc
     5041 ctgtcgaaga aacaatcttg ccgtccttca cttctacgct catcgctccc caacgtcccg
     5101 cggtgaggat tttaccgccc ttctcttctg cccatgcggg gagcggtgct gccgatgtca
     5161 ccaccagcgc tccagcggca ataccgctgt gtttaataaa ttcacgtctt gttaatgcca
     5221 taacttcctc cctgatcaac gaggatcact gtttctcggt aatatctttg gcgttgtact
     5281 ggaaataccg cgttaaaatg tccggttcgt tttcgctcat gctggttcgt gcccccattc
     5341 ctttggcaat ggacggccac gcattgacgg tgtaatggtc ggcggcaata gaggcatgac
     5401 aaccagcgca ataggtatcg tcaagttttt cagcgtattg ccatagcggt ttacggtccg
     5461 ctaatgcggg atcggtaagc gcaccctgta aagacgcctg acgccattga ttgccgtatt
     5521 cgtcagcctg ccattccccg tttaccgtga gcgccttgat accttcttca cttaatgtgg
     5581 ctagcgccag ccgttgacct gccgccaggt agagcgtgtt ttcactgccc tgcatttgat
     5641 aaccctgcaa cagaacgatc ggctgtttgc cactggcatc aacgacggtg agatcggtac
     5701 caggattcac ggtagccagc tcgcctatgc gagaagtttt gaaaggataa atatgtgcgc
     5761 cattagtcac tgaagtagcg gcctgacttt ccagctcatg cgccgcgttg tcatccattt
     5821 ttatttctgg cggaaaatgg gcaatgcctt tatgacaatc gatacaggtt tcgccgccct
     5881 tttgtgcttt gttatgcatt ttctgcgcag attcactttg cgaggcaata tccatggcat
     5941 caaaagaatg gcaactacgg cacgttgcag agtcagttgc ttttaattct ttccatactg
     6001 tttcggccat ttcctggcga tgagtttcga acttatcgtc actgtctatt ttgccgctaa
     6061 caaattcatg ataaatatct ttagatgctt ttaatttagc aaataaataa tccatccctg
     6121 actttggaat atggcaatcg gcacattccg cacgtatccc tttctggttc gaaaagtgga
     6181 cagttccctg atattcctca aaaggtttac tcatcgagtg gcaggaaagg caaaatgctg
     6241 tatccgacgt tttatgtaag gctttttgcg ccagcaataa cccgccgcca ccaaccacaa
     6301 ccgctatcag caaaaacaat aacccaatgc gttttttccc tcgtatagat tcctctaaat
     6361 aaattccaga acaccgaaat cattgtataa ttaaatacac aacgaattaa ccgcttgcat
     6421 aactcggcac aacactgcct gaaataatcg ataaagaata tgatttatta caatgtaatc
     6481 attaattgct aaggaataac ccagttgcta tttagaatat atatttatca gttttagcaa
     6541 tttaaatccc ataatcaatg tgaatataaa aaatattttt agcacaacgc tatgcgcact
     6601 gtcacccgct caacagatca cggttatcgt tcgtttttta tactgctcag ggataaaaaa
     6661 aggcccctgt tgaaattgca ggggcctggt acgagcaagc atcatattgg gcgacatgat
     6721 gcaacggtaa aaatcatttg gcctgatggc gttcaatgat tcctttcatt tcagcaaccg
     6781 ccgccccgtc tacgatataa cgcgaatact cgtccgcgtg ttcatctgat gacatggaca
     6841 atccttgatt acgataacgc atcggtgagg cttgccacgc tcccgcggag ctatggactt
     6901 ccagcactcc ggcatcgagg aagtggtgca ggttttctgc acggactcct gctccagcca
     6961 taatgattgg agcatcacga tgggcaataa gttccataat ttttgataaa ccttgcagtg
     7021 cgtctgattt ttgccctgat gtcagtaccc gggcaatgcc caattccgca agattattga
     7081 gtgtatataa agggttagcg cacatatcga aggcgcgatg aaaagtcact gccagcggac
     7141 cggcagcagc cattattttt tccattcgtg gcatatcgac attcccgtca acatcgagaa
     7201 cgcccgtcac cagtccagga aaacctaatt cgcggaccgt gcgcacatcc tcaagaatgg
     7261 cggcaaactc accgtcgctg tagcaaaaat caccaccgcg tgggcgaatt atcggatgca
     7321 cagggatcgt cacccgctgg cgcacggatt tcagtacccc caacgacggc gttaagcccc
     7381 cctcttttgg ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc gccgttagtg
     7441 cacattccat gctgtaacag caaatttcca gtaatgccat ttttgctcct taattacgct
     7501 gactgctcgc tggcaacgat ctcttcaatg gaccacggat gaaacttaat agttgttttg
     7561 ccatcggtga ccgccagcgt cggattcggt aatcgctcat gctcaccttt tggtgaactg
     7621 gttttaactg agatccccgg taggctgagt ccttcatcag aaagcaatgc cagcgcacgg
     7681 gcattcagcg tttctggatc acccggcaga acgatttcaa tatgttccca accttcgtgt
     7741 gggtaacgtt tttccccggg ccacggtagc tccacattgg aaaactgcca gtgcgcaacc
     7801 tgtaccggtt catgcaattt aaacagacaa atcggtctgc cattgatcat tttttctgac
     7861 aaaagctcgc cacactgttc aaaaccgcga cgccagcgct cagcggtgac gttttgatgg
     7921 caacgcaaag aaatgtgatc ggcagtcagc ggagtgatat ccaaccccag acggcgggaa
     7981 agttcatcta atgcgtgggt aaatcgcggt aaatccgatg caatatcctg cagctcgtcg
     8041 atagattgcc agttcgccat aatcactctt cgtctttcag taaaagcgtt aatttaccct
     8101 gttgccctgt gccaaccaac cgctgatttc acgccgcttc tgatgcaata gtgaaaacgg
     8161 caatacgcca cgcgcacgtt gctgacgaaa acagccattt gcagtatact cccgccctaa
     8221 tttctttaac tggtgcgggc aatttttgct cgcttcatca atgtaaggta ttccggtgaa
     8281 tattcaggct cttctctcag aaaaagtccg tcaggccatg attgcggcag gcgcgcctgc
     8341 ggattgcgaa ccgcagattc gtcagtcagc aaaagttcag ttcggcgact atcaggctaa
     8401 cggcatgatg gcagttgcta aaaaactggg tatggcaccg cgacaattag cagagcaggt
     8461 gctgactcat ctggatctta acggtatcgc cagcaaagtt gagatcgccg gtccaggctt
     8521 tatcaacatt ttccttgatc cggcattcct ggctgaacat gttcagcagg cgctggcgtc
     8581 cgatcgtctc ggtgttgcta cgccagaaaa acagaccatt gtggttgact actctgcgcc
     8641 aaacgtggcg aaagagatgc acgtcggtca cctgcgctct accattattg gtgacacagc
     8701 agtgcgtact ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg tcggcgactg
     8761 gggcactcag ttcggtatgc tgattgcatg gctggaaaag cagcagcagg aaaacgccgg
     8821 tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga
     8881 agatgaagag tttgccgagc gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga
     8941 atatttccgc gagatgtggc gcaaactggt cgacatcacc atgacgcaga accagatcac
     9001 ttacgatcgt ctcaacgtga cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa
     9061 cccaatgctg ccaggaattg tggcggatct caaagccaaa ggtctggcag tagaaagcga
     9121 aggggcgacc gtcgtattcc ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt
     9181 gatcattcag aagaaagatg gcggctatct ctacaccacc actgatatcg cctgtgcgaa
     9241 atatcgttat gaaacactgc atgccgatcg cgtgctgtat tacatcgact cccgtcagca
     9301 tcaacacctg atgcaggcat gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt
     9361 accgctggaa caccacatgt tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac
     9421 ccgcgcgggt ggtaccgtga aactggcaga cctgctggat gaagccctgg agcgtgcacg
     9481 tcgtctggtg gcagaaaaga acccggatat gccagctgac gagctggaaa aactggctaa
     9541 cgcggttggt attggtgcgg tgaaatatgc ggatctctcc aaaaaccgca ccacggacta
     9601 catcttcgac tgggacaaca tgctggcgtt tgagggtaat accgcgccat acatgcagta
     9661 tgcttacacg cgtgtattgt ccgtgttccg taaagcagaa attaacgaag agcaactggc
     9721 tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca
     9781 gtttgaagaa accctcaccg tggttgcccg tgaaggcacg ccgcatgtaa tgtgtgctta
     9841 cctgtacgat ctggccggtc tgttctctgg cttctacgag cactgcccga tcctcagcgc
     9901 agaaaacgaa gaagtgcgta acagccgtct gaaactggca caactgacag cgaagacgct
     9961 gaagctgggt ctggatacgc tgggtattga gactgtagag cgtatgtaat cgatttttcg
    10021 tgagagtgaa gcctgatcag ggttagccga tcaggctttt ttattgccat ctaaatgtat
    10081 tctgaaaacg gacatgccat tgttttctca ctgttgagta agaggccaga agcgtaatat
    10141 ccaggcccag gaaaacgata acggttcaat ttaaggaata ccgaagtgtt taaatttctt
    10201 gtattaacat taggcattat ctcttgccag gcttacgcag aagatacggt tatagtaaac
    10261 gaccatgaca cttcagccat caaagattgt tggcaaaaaa attcagatga tgatactgac
    10321 attaacgtga tcaaatcatg cctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat
    10381 aaagcctatg gtgaagctta tcgttatata gaacaagtgc cacgcacagg tgcaaaaaaa
    10441 cttgatactg aacaacttaa cttgcttaaa aaatcacagc gagcctggct ggattttagg
    10501 gacaaagaat gtgaattaat cctttcaaat gaggatgttc aggatttaag tgacccctat
    10561 tctgaatcag aatggctatc atgtatgatc atacagacca atacgcgaac tcgccagttg
    10621 cagctatacc gtaactctga agatttttat ccaagccctt tgacaagagg ataattcaca
    10681 tctttttggc atgttttgtt gcaagctatt cctgataaat gattgcaaca aaacatccag
    10741 ccttttgact gagttattaa acatactcgc gagcgcgtaa ttttttgtct ttcagcgata
    10801 attcacaatc acttcattcc gttgtacctt tagcggtgga attaaccgc
//
LOCUS       DAJVRP010000119        10753 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVRP010000119 DAJVRP010000000
VERSION     DAJVRP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.119"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(24..1052)
                     /gene="malI"
                     /locus_tag="OQN12_003455"
     CDS             complement(24..1052)
                     /gene="malI"
                     /locus_tag="OQN12_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1655657.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1227..2819
                     /gene="malX"
                     /locus_tag="OQN12_003456"
     CDS             1227..2819
                     /gene="malX"
                     /locus_tag="OQN12_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1655658.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2829..4001
                     /gene="malY"
                     /locus_tag="OQN12_003457"
     CDS             2829..4001
                     /gene="malY"
                     /locus_tag="OQN12_003457"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1655659.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4105..5106
                     /gene="add"
                     /locus_tag="OQN12_003458"
     CDS             4105..5106
                     /gene="add"
                     /locus_tag="OQN12_003458"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1655660.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5142..6182)
                     /locus_tag="OQN12_003459"
     CDS             complement(5142..6182)
                     /locus_tag="OQN12_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1655661.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6425..6550
                     /gene="blr"
                     /locus_tag="OQN12_003460"
     CDS             6425..6550
                     /gene="blr"
                     /locus_tag="OQN12_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1655662.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6823..7038
                     /gene="ydgT"
                     /locus_tag="OQN12_003461"
     CDS             6823..7038
                     /gene="ydgT"
                     /locus_tag="OQN12_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1655663.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7124..7564
                     /locus_tag="OQN12_003462"
     CDS             7124..7564
                     /locus_tag="OQN12_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1655664.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7641..8222
                     /gene="rsxA"
                     /locus_tag="OQN12_003463"
     CDS             7641..8222
                     /gene="rsxA"
                     /locus_tag="OQN12_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1655665.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8222..8800
                     /gene="rsxB"
                     /locus_tag="OQN12_003464"
     CDS             8222..8800
                     /gene="rsxB"
                     /locus_tag="OQN12_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1655666.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8793..>10753
                     /gene="rsxC"
                     /locus_tag="OQN12_003465"
     CDS             8793..>10753
                     /gene="rsxC"
                     /locus_tag="OQN12_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1655667.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAA"
BASE COUNT         2486 a         2790 c         2747 g         2730 t
ORIGIN      
        1 aagtatagca gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga
       61 ttgcgtgaat gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtgata
      121 ccaagttccc gcgctggcgt gctggcccag gtaacaggta tatcatcaag tgtggttggt
      181 gtcgcatcgg taaatgccgc cagcgaaacc tgttgctcaa agtaacgatc gactccgctt
      241 tccccgcttt gccgccctgc tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg
      301 ttatagcaaa ccacggcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc
      361 gcggcttgct tctggctgga agtgcactcc aacacccaat cgctgtgaaa cggcaggcca
      421 aattttagta gagttgcaca atagcccccc acccgttctg cacgggttaa tgaggaactt
      481 tgccctccca gccaggcgat ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac
      541 tgtgcagcct gcatgttgtc cgggcgaacc gtatcaacat catcaagata actggcacgg
      601 gaagcgaaaa tcaccgggat agctttttct tctgccatcc gccgcaggtc atcgctgctt
      661 cctgcagccc cggcaattac cacaccatcg acaccctgat tcagtaacag tgaaaaccgc
      721 tgtgccagct gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgtgct
      781 tccagagctt ccgtcaatcc ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg
      841 acgatcaaac caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg tcgattgcgc
      901 acaaatccca gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc
      961 cctttgccat taagcaccag cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca
     1021 tcatgaatgg ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga
     1081 cagataaaac gttttacctt ttatttatct tatacccgct attatcgttg cgtaatgtga
     1141 tttatgactc actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca
     1201 gattgttctc tacgaggagt cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt
     1261 gggagttctt ccagcagtta ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct
     1321 gcggcattat gctcggcatt ggtagttctc ttagcagcca tgatgtcata accctgatcc
     1381 cggtcctggg caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt
     1441 ttgcttttag tttcctgcct gtcatgttct gtatcgccat cccgctgggc ctggcacgcg
     1501 aaaataaagg cgtagcggca ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg
     1561 taaacttctg gttgaccaat aaaggcattc tgccaaccac agatgccgcc gttctgaaag
     1621 ccaataacat ccagagcatt cttgggatcc agtcgatcga taccgggatc ctcggtgcgg
     1681 tgatcgccgg tattatcgtc tggatgctgc atgagcgttt ccataatatc cgcctgccgg
     1741 atgcgctggc attcttcggc ggtacgcgct tcgtaccaat tatctcctcg ctggtgatgg
     1801 gccttgtcgg cctggtgatt ccattagtct ggccgatttt cgccatgggt attagcggct
     1861 tgggccatat gataaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac
     1921 gtctgctgtt gccgtttggt ctgcatcaca ttctggtggc attaattcgc tttaccgacg
     1981 caggcggcac gcaggaggtc tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg
     2041 cgcaattgag ttgcccgacc actcacggtt tttctgaaag cgtcacgcgt ttcctttcgc
     2101 aaggtaaaat gcctgcgttt ctcggcggtc taccaggtgc agcgttagct atgtatcact
     2161 gcgcgcgccc ggaaaatcgc cataaaatta aaggtctgct gatttctggc ctgatcgcct
     2221 gcgtcgttgg cggcactacc gaaccgctgg aattcctgtt cctgttcgta gcgccagttc
     2281 tgtatgtcat ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg
     2341 tcaccatcgg taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg
     2401 gtctgtcaac caagtggtac atggtgccag tggtggcggc aatctggttt gtcgtttact
     2461 acgtcatctt ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg
     2521 aagttgccag ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg
     2581 ttcctgcaat cctcgaagca ttaggcggtg ccgacaatat tgtcagcctc gataactgca
     2641 ttacccgtct gcgtttgtct gtggaagata tgtcgcttgt taatgtgcag gcactgaagg
     2701 acaatcgggc aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc
     2761 cacaagttca gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtc caggcataag
     2821 gataagatat gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt
     2881 gggattatgt cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca
     2941 tggattttgc cactgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg
     3001 tatttggcta cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt
     3061 ccacccagca ttacaccgca attgatacgc agacggtggt gtatggccct tctgtcatct
     3121 atatggtttc agaactgatt cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca
     3181 cgcccgccta tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg
     3241 ttgctttaga gaagcaggct gacggctggt tttgcgatat gggcaagctg gaagccgttt
     3301 tggcgaaacc agaatgtaaa attatgctcc tgtgtaaccc acagaatcct accgggaaag
     3361 tgtggacgtg cgatgagctg gagatcatgg ctgacctgtg cgagcgtcat gatgtgcggg
     3421 ttatttccga tgaaatccat atggatatgg tctggggcga gcagccgcat attccctgga
     3481 gtaatgtggc gcggggagac tgggcgttgc tgacatcggg atcgaaaagt ttcaatattc
     3541 ccgcgctgac cggtgcttac gggattatag aaaatagcag cagccgtgat gcctatttat
     3601 cggcactgaa aggacgtgat gggctttctt ccccttcggt actggcgtta actgcccata
     3661 tcgccgccta tcagcaaggt gcaccgtggc tggatgcctt acgcgtctat ctgaaagata
     3721 acctgacgta tatctcagat aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc
     3781 cgcaatccac ttatctggcc tggcttgatc tacgtccgtt gaatattgac gacaacgcgt
     3841 tgcaaaaagc gcttatcgag caagaaaaag tcgcgatcat gccggggtat acctatggtg
     3901 aagaaggtcg tggttttgtc cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag
     3961 gtgtggctgg attaataaac gccatccgcg ctgttcgcta accccaattg cgcaacgtaa
     4021 aaaatcgttg cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt
     4081 tatatccaga aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat
     4141 cgccaccttg atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat
     4201 atctcgcttc ctgcacaatc ccttgaaaca ctgattcccc acgttcaggt cattgccaac
     4261 gaacccgatc tggtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct
     4321 cttgatgcct gccgccgcgt ggcatttgaa aacattgaag atgcagcccg taacggcctg
     4381 cgctatgtcg agctgcgttt ttcaccaggc tacatggcaa tggcacataa gctgcctgta
     4441 gcgggtgttg tcgaagcggt gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg
     4501 caggcgaagc ttatcggcat tatgagccga accttcggcg aagccgcctg tcagcaagag
     4561 ctggaggcct ttttagccca ccgtgaccag attaccgcac ttgatttagc tggtgatgaa
     4621 cttggattcc cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg
     4681 catattaccg tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt
     4741 gaactgggtg cggagcgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg
     4801 gattttctcg ccgagcaaca aattggtatt gaatcctgtc tgacctccaa tattcagacc
     4861 agcaccgtag cagagctggc tgcacatccg ctgaaaacgt tccttgagca tggcattcgt
     4921 gccagcatta acactgacga tcccggcgta cagggagtgg atatcattca cgaatatacc
     4981 gttgccgcgc cagctgctgg gttatcccgt gagcaaatcc gccaggcaca gattaatggt
     5041 ctggaaatgg ctttcctcag cgcagaggaa aaacgcgcac tgcgagaaaa agtcgccgcg
     5101 aagtaacaaa aatggatggt gcaaattgca ccatccattt ttcacgcaag gcacaacgtc
     5161 gcgcgatgtt tggcggattc gatgcccagc tcaatcaact ccattacctg gattgcctgg
     5221 cttgccggaa ccggattttc accatcgcca tttaacgcat cacgaatcgc cgcatagtaa
     5281 gccggataat tcccaggcac cgtcaacagc gtttcttcga cacgttcctc accttccacg
     5341 cgggtaagta cgccatcacg catatcgtag ccccagtctt cctgcggcag acgctcgcca
     5401 tttttcagac gttcttcctg tggatcgagg ccatatttca cataactgcc tcgggatcca
     5461 tgcacgatat accgtgctga ctcagcggct gccagcatgg taccgtgtaa aatgactcgc
     5521 cgctgtggat aggacaagat ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac
     5581 tgtgccaaat ctaccgtcat gctgaccggt aaaccaaata gcgtaatggc ctgatcaaga
     5641 agatgcggtg ctaaatcgta ccagataccg ctgcctgggc cgccctgttc acgccaacga
     5701 tcgcgcacct gcggacggaa gcggtcaaaa tgagactcaa agtaagcaac ttcacccagc
     5761 acgccttccg cgagcaaacc ttttagcgtc aagaaatcgc tatcccaacg acggttatgg
     5821 aatacagaca gcacacgccc caggcttttt gccagcgcat ccagctctcg cgcttgtgac
     5881 agtgtcacgg taaagggttt atcaacgacc acatatttac ccgcctcaag cgccgctttg
     5941 gctaacggga aatgggtatc gttgggtgta ggaatgacaa tcaggtctat gttgggatcg
     6001 ttaaacagat gcttcggctc agagacaacc gtaaccgttg gccagtcggc ttttactttt
     6061 gtttcatcac tgctggagat taccgccagt tccagcccgg gcgtgcccgc aatcaggggc
     6121 gcatggaagg ttttgctcgc ataaccatac ccaatcaacc caacacggat gttgtcgctc
     6181 atgtcattgc ctctcattga agtatgatgg ctatttgaca ctatccttta cccacgctca
     6241 acagtttaat aacctgccag caataaggga tgttgtttaa cttaagtcaa aaaaatagcg
     6301 aattttccaa cgacaaaagc taaatatcgc aaaaacctca gtaaaaatct tgctggagct
     6361 attattgcta agtaacattt accccctgaa gttaatggat caatcaagag agatgtgggc
     6421 tgtaatgaat cgtcttattg aattaacagg ttggatcgtt cttgtcgttt cagtcattct
     6481 tcttggcgtg gcgagtcaca ttgacaacta tcagccacct gaacagagtg cttcggtaca
     6541 acacaagtaa gctctgcact tgtggagcga catgctgccc gtccgggtgc atgttttcac
     6601 ttgtcggata ttaaaccagg aatttattat cttgttcgat gttgttggtg attgtcaggg
     6661 atagtaaagc gcgttactct tttcagagca gtttttcatc cctgaatatt tgccatcagc
     6721 gcattcttat tattgtaata acacaagcgt tcacttttgg tgaaatttta tcatcactaa
     6781 taatacctac ccctttggta ttaattattt tatggacctt ttatgactgt tcaggactac
     6841 ttattaaaat ttcgcaaaat cagttcactc gaaagtctgg aaaaactcta cgaccatctt
     6901 aattacaccc tgacggacga tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt
     6961 cgcgcagagc tggtttctgg cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc
     7021 tggcactatg tccaataaag aaagtagcga tcggttataa aagccttata ataacgcccc
     7081 taaaattaga tttgcctgtt tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc
     7141 acaacgtatt ggaggctggt tgcttggccc gttggcctgg cttttagtcg ctctgttgag
     7201 tacgacgctg gcgttgttgc tgtataccgc tgcgttatct tctccgcaaa catttcaaac
     7261 gctcggcgga caagcactga cgacacaaat cttatggggc gtttctttta ttaccgctat
     7321 cgccatgtgg tattacacac tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt
     7381 tcctaaacac tatattatct ggctgcttat ttccgtattg ctggcagtaa aagcattcgc
     7441 tttttcaccc gtggaagttg gcatagccgt tcgccagttg ctgtttactt tgttggcaac
     7501 cgcattgatc gtcccctatt tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc
     7561 gtaataacct tacagttaac ctgttgtcgc ctgctctgga ttaacggata ataggcggct
     7621 tttttatttc aggccgaaaa atgactgact acctgttact gtttgtcgga actgtactgg
     7681 tcaataactt tgtactggtc aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa
     7741 aactggaaac cgcgatgggc atggggctgg caacaacgtt tgtgatgacg ctgacgtcta
     7801 tttgcgcctg gcttatcgat acgtggattt tgatcccact taatctgatt tacctgcgca
     7861 ccctggcatt tattctggtg attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca
     7921 aaaccagccc ggtgctttac cgtctgctgg ggattttttt gccgcttatc accaccaact
     7981 gtgcagtgct cggcgtggcg ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg
     8041 cgctgtacgg tttttccgcc gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca
     8101 tccgcgaacg ccttgctgtg gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt
     8161 taattaccgc aggtcttatg tctctggcct ttatgggctt tagtggtttg gtgaagttgt
     8221 aatgaatgct atctggattg ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc
     8281 cattctgggt tatgcctccc gccgttttgc ggtggaagac gatccggtcg ttgagaaaat
     8341 tgacgaaatc ttaccgcaga gccagtgtgg tcagtgcggt tatcccggct gtcgccccta
     8401 cgcggaagcc atcagctgta acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc
     8461 tgtgatgcta aaaattgccg agttgcttaa tgtcgagccg cagccgctgg atggcgaagc
     8521 gcaagagcta acgcctgcgc ggatggtggc ggttattgat gaaaataact gtattggctg
     8581 cactaaatgt attcaggctt gtccggtaga cgccatcgtt ggcgctaccc gtgtcatgca
     8641 tacggtaatg agtgatctct gtacgggctg caatttatgt gttgatccgt gcccgacgca
     8701 ctgcatctcg ttgcaaccgg tcgcagaaac acctgactcc tggaaatggg atctgaacac
     8761 cattcccgtg cgtatcattc ccgtggaaca ccatgcttaa gttattctct gcattcagaa
     8821 aaaataaaat ctgggatttc aacggcggca tccatccacc ggagatgaaa acccagacca
     8881 acggtacccc cctgcgccag gtactactgg cgcagcattt tgttattcca ctgaaacagc
     8941 atattggcgc tgaaggtgag ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc
     9001 cgcttacccg tggtcgcggc aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta
     9061 cggctattgc gccccactct acggctcatc cttcagcttt agctgaatta agcgtgatta
     9121 ttgatgccga tggtgaagac tgctggatcc cgcgcgacgg ctgggtcgat tatcgctctc
     9181 gcagtcgcga agagttaatc gagcgcatac atcagtttgg tgttgccggg ctgggcggtg
     9241 ccggattccc gacaggcgtt aaattgcagg gtggtggaga taagattgaa acgttgatta
     9301 tcaacgctgc tgagtgcgag ccgtacatta ccgccgatga ccgtttgatg caggattgcg
     9361 cggctcaggt cgtagagggt attcgcattc ttgcgcatat tctgcagcca cgcgaaattc
     9421 ttatcggcat tgaagataac aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg
     9481 actctaacga tatttctctg cgggtgattc caaccaaata tccttctggc ggtgctaaac
     9541 aattaaccta cattctgacc gggaagcagg ttccacatgg cgggcgttca tccgatatcg
     9601 gcgtattaat gcaaaacgtc ggcactgctt atgcagtgaa acgtgccgtt attgatggcg
     9661 agccgattac cgagcgtgtt gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg
     9721 tctgggcacg gctggggacg ccagtgcgtc atttattgaa tgatgccgga ttctgcccct
     9781 ctgccgatca aatggtgatt atgggtggcc cgctaatggg ctttaccttg ccatggctgg
     9841 atgtcccggt cgtaaagatt accaactgtc tgttggctcc ctctgccaat gaacttggcg
     9901 aaccacagga agaacaaagc tgcatccggt gtagcgcctg tgctgacgcc tgccctgtgg
     9961 atcttttgcc gcaacagttg tactggttca gcaaaggtca gcaacacgat aaagctacca
    10021 cgcataacat tgccgactgc attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata
    10081 ttcccttggt gcaatatttc cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag
    10141 aaaagcgcgc cgcagaagcc aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg
    10201 aaaaagcgac tcgccttgaa cgacataaga gcgcagccgt tcaacctgca gccaaagata
    10261 aagatgcgat tgctgccgct ctggcgcggg tgaaagagaa acaggcccag gctacacagc
    10321 ctattgtgat taaagcgggc gaacgcccgg ataacagtgc aattattgca gaacgggaag
    10381 cccgtaaagc gcaagccaga gcgaaacagg cagaactgga gcaaactaac gacgcagcaa
    10441 ccgttgctga accacgtaaa actgccgttg aagcagctat cgcccgctcc aaagcgcgca
    10501 agctggaaca gcaacaggct aatgcggaac cagaagaaca ggtcgatccg cgcaaagccg
    10561 ccgtcgaagc cgctattgcc cgtgccaaag cgcgcaagct ggaacagcaa caggctaatg
    10621 cggaaccaga agaacaggtc gatccgcgca aagccgccgt cgaagccgct attgcccgcg
    10681 ccaaagcacg caagctggaa cagcaacagg ccaatgcgga gccagaagaa caggttgatc
    10741 cgcgcaaagc tgc
//
LOCUS       DAJVRP010000120        10665 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVRP010000120 DAJVRP010000000
VERSION     DAJVRP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.120"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(51..1400)
                     /gene="lysC"
                     /locus_tag="OQN12_003466"
     CDS             complement(51..1400)
                     /gene="lysC"
                     /locus_tag="OQN12_003466"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1655668.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            1925..3574
                     /gene="pgi"
                     /locus_tag="OQN12_003467"
     CDS             1925..3574
                     /gene="pgi"
                     /locus_tag="OQN12_003467"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1655669.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4036..4278
                     /locus_tag="OQN12_003468"
     CDS             4036..4278
                     /locus_tag="OQN12_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655670.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4392..5030
                     /locus_tag="OQN12_003469"
     CDS             4392..5030
                     /locus_tag="OQN12_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1655671.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5027..5764
                     /locus_tag="OQN12_003470"
     CDS             5027..5764
                     /locus_tag="OQN12_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1655672.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5764..7860
                     /locus_tag="OQN12_003471"
     CDS             5764..7860
                     /locus_tag="OQN12_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1655673.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7907..8185)
                     /locus_tag="OQN12_003472"
     CDS             complement(7907..8185)
                     /locus_tag="OQN12_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655674.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8186..8329
                     /locus_tag="OQN12_003473"
     CDS             8186..8329
                     /locus_tag="OQN12_003473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655675.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8455..8865
                     /gene="psiE"
                     /locus_tag="OQN12_003474"
     CDS             8455..8865
                     /gene="psiE"
                     /locus_tag="OQN12_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS1655676.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8909..10384)
                     /gene="xylE"
                     /locus_tag="OQN12_003475"
     CDS             complement(8909..10384)
                     /gene="xylE"
                     /locus_tag="OQN12_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCS1655677.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2710 a         2722 c         2763 g         2470 t
ORIGIN      
        1 gacaagaaaa tcaatacggc ccgaaatata gcttccaggc catacagtat ttactcaaac
       61 aaattactat gcagtttttg caccacctgc tcggcatctt cgccgggcac caggaagcac
      121 aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg
      181 aatacctctt tgccaacgcc gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc
      241 gccagacctt cttccacctc cacccggcac agtgcggaaa gctccatcag cagagattgc
      301 gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat caagggttaa tgccacgctc
      361 acttctgacg tggtgattaa gtctaccgaa atattatgcc gcgcgaggat gccgaaaact
      421 tccgcgagga agccgcgaga atgcagcata ttcaggctgt gcaaagtgag cagagtctga
      481 ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt cagttttatt gcacaccagc
      541 gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga ccgggatatc gctgcgtact
      601 gcgggtagca acgttgccgg atgcagtact tttgcaccaa aagttgccat ctctgccgct
      661 tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg
      721 tagatgcccg ggacgtcggt ccagatatca acacgagatg cgtgtaaagc ctccgccagc
      781 aaggctgccg tataatcgct gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg
      841 ctaccgataa atccctgggt gatcactaag ccttcattga ggcgtgggag cagctgcagc
      901 gcggccagtt ccgccagcgc ggctacatct ggctctgcac gaccaaatcg gtcgttggta
      961 cgcatcactt tacgtacatc aaaccactgt gcctgaacat cgcgttcgcg caggatttcg
     1021 acaaacagca gggtcgacat cagctcgccg tggctgacca gctcatctgt cagcgccgga
     1081 gacgttgcca gcgccgccgc ttctgccaga acagtaatgt tctccagcag acgttcaatc
     1141 tcttcacgga taacgttcgg gtaacgcaga cgttccagaa tggcaaactg gatgttgcga
     1201 atagcgtcga gtttttcgaa tcgctcgcca agttccagcc cttccgctaa agcgaccagc
     1261 agattagtga taccagcaga agccgagagg actactaaac gcacgttggc atcagaaagc
     1321 acaatatcag cgctgcggtt catggcgtca aaatcagcta cgctggtacc gccaaatttg
     1381 gagacaacaa tttcagacat aactacctcg tgtcagggga tccattttca gccttggcac
     1441 aagggaagag cggaagacgg gtgggcgcag agcgatactt cgctactatt ttcacccaga
     1501 agtgctccac cacttgcgaa acgcccgact gcgaacgctt ctggtgacaa cccaggggat
     1561 tcagcccctg tagccgatga tgaacgtggc cagccgttca atcacctcgg cgatgcaccc
     1621 cctcaggtgt tatcacagga ctggctcctc caacaccgtt acttgggcaa cgcgcctctt
     1681 ctggcctgcg ctagcgcagg tagtacattt ataaataaag ggtgagcggg gcggttgtca
     1741 acgacggggt catgcggatt tttcatccac tcctggcggt cagtagttca gctaataaat
     1801 gcttcactgc gctaagggtt tacactcaac attacactaa cggcactaaa accatcacat
     1861 ttttctgtga ctggcgctac aatcttccaa agtcacaatt ctcaaaatca gaagagtatt
     1921 gctaatgaaa aacatcaatc caacgcagac cgctgcctgg caggcactac agaaacactt
     1981 cgatgaaatg aaagacgtta cgatcgccga tctttttgct aaagacggcg atcgtttttc
     2041 taagttctcc gcaaccttcg acgatcagat gctggtggat tactccaaaa accgcatcac
     2101 tgaagagacg ctggcgaaat tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat
     2161 taagtcgatg ttctctggcg agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt
     2221 agcgctgcgt aatcgtagca ataccccgat tctggttgat ggcaaagatg ttatgccaga
     2281 agtcaacgcg gtgctggaga agatgaaaac cttctcggaa gcgattatct ccggtgagtg
     2341 gaagggttat accggcaaag caatcactga cgtagtgaac atcgggatcg gcggttctga
     2401 cctcggcccg tacatggtga ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca
     2461 ctttgtttct aacgtcgatg ggactcacat ctcggaagtg ctgaaaaaag taaacccgga
     2521 aaccacgctg ttcctggtag catctaaaac cttcaccact caggaaacca tgaccaacgc
     2581 ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca
     2641 ttttgcggcg ctctcaacca atgccaaagc cgttggcgag tttggtattg atactgccaa
     2701 catgttcgag ttctgggact gggtcggcgg ccgttactct ttgtggtcag cgattggcct
     2761 gtcgattgtt ctctccatcg gctttgataa cttcgttgaa ctgctttctg gcgcacacgc
     2821 aatggacaag catttctcca ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct
     2881 gattggcatc tggtacaaca atttctttgg tgcggaaact gaagcgattc tgccgtatga
     2941 ccagtatatg caccgtttcg cggcgtactt ccagcagggc aatatggagt ccaacggtaa
     3001 gtatgttgac cgtaacggta acgttgtgga ttaccagact ggcccgatta tctggggtga
     3061 accaggcact aacggtcagc acgcgttcta ccagctgatc caccagggaa ccaaaatggt
     3121 accgtgcgat ttcatcgccc cggctatcac ccataacccg ctctctgatc accaccagaa
     3181 actgctgtct aacttcttcg cccagaccga agcgctggcg tttggtaaat cccgcgaagt
     3241 ggttgagcag gaatatcgcg atcagggtaa agatccggca acgcttgact acgtggtgcc
     3301 gttcaaagtg ttcgaaggta accgcccgac caactccatc ctgctgcgcg aaatcactcc
     3361 gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa atctttactc agggcgtgat
     3421 cctgaacatc ttcaccttcg accagtgggg cgtggagctg ggtaaacaac tggcaaaccg
     3481 tattctgcca gagctgaaag atgataaaga aatcagcagc cacgatagct cgaccaatgg
     3541 tctgattaac cgctataaag cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat
     3601 gtaggccgga taaggcgttt acgccgcatc cggcaaccgg agcctgatgc caatatcgac
     3661 attaatgcat agtatgagga gttaaataac cttaaaaaca atgccgacaa taagtcggca
     3721 ttgttttatc agataaatcc ccttgtctgt gatttaacgg aaatcatacc gtgaggttaa
     3781 tcctaaaata gatttttaat cgttgtttat ttcggagaat acgcagatta attacttttg
     3841 tttttatttt aagcttatga tttttattgt tatttaaata taagttgaaa cttatatttg
     3901 atattcattc caattatcct aaaactccat cgctaattcc ccgcgccgta attcgcatgc
     3961 tttagttgtg tatactcgat cccgcccgaa atgtttttgg gtaaatctcc attcattcaa
     4021 tgaagggaaa ttgttatgaa aaaagttctg tatggcattt ttgccatatc tgcgcttgcg
     4081 gcgacttctg cgtgggctgc acctgtacag gtgggcgaag cggcagagtc ggcagcaacg
     4141 tcggtttcgg cggggagttc ctccgcgacc agcgtcagca ccgtaagctc ggcggtgggt
     4201 gtcgcgctcg cggcaaccgg tggcggtgat ggttctaata ccgggaccat cacaaccacg
     4261 accaccagta cccagtaata aagtatgtat ccccaaaata atttgagtca ttgcatctgt
     4321 ggcttgaagt atgaagggat ttaccataac cacactccgg tgtggttatt ctgcccctct
     4381 ggagaagagt cgtgaagcga cctgcactca ttcttatctg cctgctatta caggcctgtt
     4441 cagccacgac taaagagctg ggcaattcac tgtgggacag tctgttcggc acgccaggcg
     4501 tacagctgac ggacgatgat attcaaaata tgccctacgc cagccagtac atgcagctta
     4561 atggcgggcc gcaattgttt gtggtgctgg ccttcgctga agacggacaa caaaaatggg
     4621 tcactcagga tcaagccact cttgtcacac aacatggtcg tctggtgaag actttgcttg
     4681 gcggcgacaa cctgattgaa gtgaataacc ttgccgccga cccgctgatt aaacccgcac
     4741 aaattgttga tggtgcaaca tggacgcgca cgatgggctg gaccgagtac cagcaggtac
     4801 gctacgccac cgcacgctca gtcttcaaat gggatggcac cgataccgtc aaggtcggca
     4861 gcgatgaaac cctggttcgc gtgctggacg aagaagtctc caccgaccag gcgcgctggc
     4921 ataaccgcta ctggatcgac agcgaagggc aaattcgcca gtcggaacag tatctcggcg
     4981 cggattattt cccggtgaaa accactctca tcaaggcggc aaaacaatga ttaaacaaac
     5041 tattgtcgcg ttgcttttga gtgtgggagc gtcatcggtc ttcgcggcag gaaccgtcaa
     5101 ggtgttcagc aatggcagca gtgaggccaa aacgctaacg ggcgcagagc atttaatcga
     5161 tctggtaggg caacctcgac tggcaaacag ctggtggcct ggtgcggtga ttagcgaaga
     5221 gctggcaacg gcggcagcat tgcgtcagca gcaggcgttg ctgacacggc tggcagaaca
     5281 gggcgcagat tccagcaccg acgatgccgc cgccattaac gccttacgcc agcaaatcca
     5341 ggcattgaaa gtgacaggca ggcaaaaaat caatcttgat cccgatatcg tccgcgttgc
     5401 cgaacgcggt aacccgccgt tgcagggcaa ctacacgctg tgggtcggac caccgccgtc
     5461 cacggtcacg ttgttcgggc ttatcagcca tcctggcaat cagccattca ctccaggtcg
     5521 cgacgtggcg agctatctct ctgaccagag cctgctcagc ggtgcggatc gcagctacgc
     5581 gtgggtcgtt tacccggacg gacgcacgca aaaagcgccg gtggcttact ggaacaagcg
     5641 tcacgtagag ccgatgcccg gcagcattat ttatgttggc ctcgcagatt ccgtctggag
     5701 tgagacgcct gatgccctta acgccgacat tcttcagact ctgacgcagc ggatacccca
     5761 ataatgaaaa aaagacatct gcttagctta ctggcgctgg gcattagcac agcttgctac
     5821 ggcgaaacat atcctgcgcc cattggtccg tcgcagtcgg atttcggtgg cgtaggatta
     5881 ttacaaactc ccaccgcgcg tatggcacgg gaaggggagt tgagtctgaa ctatcgcgat
     5941 aacgatcagt accgttatta ctcagcttca gtgcaactct tcccgtggct ggaaacaacg
     6001 ctgcgctaca ccgacgtgcg cacccggcag tacagcagcg tcgaagcgtt ctctggcgat
     6061 caaacgtata aagataaagc cttcgatctc aaactgcgtt tgtgggaaga gagttactgg
     6121 ctgccgcaag tggcggttgg tgcgcgggat atcggcggta cggggctgtt tgatgcggaa
     6181 tatcttgttg ccagcaaagc ctgggggccg ttcgatttta cgctcggcct gggctgggga
     6241 tatctgggca ccagcggtaa tgtaaaaaat ccgctctgtt cagccagtga taaatattgc
     6301 tatcgcgata acagctacaa acaggcggga tctatcgacg gtagccagat gttccacggt
     6361 cctgcctcac tgtttggcgg cgtggaatac cagacgccct ggcaaccgct gcgtctgaaa
     6421 ctggagtatg aaggtaataa ttatcagcag gattttgccg ggaagctgga gcaaaaaagt
     6481 aagtttaacg tcggggcgat ttatcgcgtt accgattggg ccgacgttaa ccttagctat
     6541 gaacgtggca acacctttat gtttggcgtc acgctgcgca ccaactttaa cgatctgcgc
     6601 ccgtcctaca tcgataacgc ccgcccgcaa tatcaaccgc agccgcagga tgccattttg
     6661 cagcattcgg tagtggcgaa tcagttaacg ttgctgaaat acaacgccgg acttgccgat
     6721 ccgcagatcc aggcgaaagg cgatacgctg tatgttaccg gcgagcaggt gaaatatcgt
     6781 gattcgcgcg aagggatcat ccgtgctaat cggatcgtga tgaacgatct gccggatggg
     6841 atcaaaacga tccgcattac ggaaaatcgc cttaacatgc cgcaagtgac gacggaaacc
     6901 gatgtcgcca gcctgaaaaa tcatctcgcc ggagagccgt tgggccacga aacgacgctg
     6961 gcgcacaaac gcgtcgagcc agtggttccg aagtccaccg agcagggctg gtatatcgac
     7021 aaatcacgct ttgatttcca tatcgatccg gtgctgaacc agtcggtcgg tggcccggaa
     7081 aacttttaca tgtatcagct gggcgtgatg ggaacggcag atttgtggct gacggaccat
     7141 ctactgacca ccggcagcct gtttgcaaat cttgccaaca actacgacaa gtttaactac
     7201 accaatccac cgcaggactc gcacttaccg cgcgttcgta cccatgtgcg cgagtatgtg
     7261 cagaacgatg tctatgtgaa taacctgcaa gccaactact tccagcatct gggcaacggc
     7321 ttctacggtc aggtctatgg aggttatctc gaaaccatgt ttggcggtgc gggggccgaa
     7381 gtgttgtatc gtccgctgga cagtaactgg gcgtttggtc tggatgccaa ctacgttaaa
     7441 cagcgcgact ggcgtagtgc aaaagatatg atgaaattca ccgactacag cgtgaaaacc
     7501 ggacatctga ccgcctactg gacgccatct ttcgcccagg acgtgttggt gaaagccagc
     7561 gtcgggcagt atctggcagg ggataaaggc ggcacgctgg agatcgctaa acgctttgac
     7621 agcggcgtgg tggtgggtgg ctatgccacg atcaccaacg tttcgaaaga ggagtacggc
     7681 gaaggggact tcaccaaagg cgtgtatgtc tcggtaccgt tggatctctt ctcgtctggc
     7741 ccgacacgca gccgtgcggc gattggctgg acgccgctga cgcgtgacgg tggtcagcaa
     7801 cttgggcgta agttccagtt gtatgacatg accagcgacc gtagcgtaaa tttccgctaa
     7861 gtcatgggaa aggtgccagt tttcgcactc actggcaccc tcattcttaa tgacctgttg
     7921 agccatcagg aacgcagttc acccccggtt tacgatctgg tgtgtaaagg cgaaaacatt
     7981 ctgtcttcct gtccgtcgat atcggttgca ggtctggttg cctgagcgac tgggccatca
     8041 ttgggctgga gacattgctt tgcggtaggt cggctttatc agcagtaccg agcggaccag
     8101 cataagcagg aagaacagag actgataaca tcaaagcagc aaaataaggc ttcattttta
     8161 ccacctttat caggttacgt ttcatttgtt ccagaggaac attgtcgatt tttcgcgcat
     8221 tgctggtggc tgggaatcac ctgaatgggt gatttttgaa ttaccggctt tggtgcggtt
     8281 tgtcttgccg gatgcgccgc caggcgcggc ttatccggcc tacgggtagg tatatccggc
     8341 tttggtggag gcgcgctcca aatccaggtt gaacaaaaca tacacaaaaa atatagatct
     8401 ccgtcacatt tttgcgttat acaggaagct cgccactgtg aaggaggtac tgctatgacg
     8461 tcactctctc gtccgcgcgt ggagtttatc tccaccattt tgcagaccgt actcaatctt
     8521 ggcctgctgt gcctcggcct gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg
     8581 gctgatgtgc tgttcgcgcc agaacaaacc agcaaatatg agctggtaga aggactggtg
     8641 gtttactttc tctatttcga atttatcgcg ctgattgtga agtactttca gtccggtttt
     8701 cacttcccgc tacgttactt tgtctatatt gggatcaccg ccattgtgcg cttgatcatc
     8761 gtcgatcata aatcgccact ggatgtgttg atctactcgg ctgcgatcct gttactggtg
     8821 atcaccctgt ggctgtgtaa ttcgaaacgg ctgaagcggg agtaaaaaga cagcacgccg
     8881 aaatggcgcg gcgtgctgga caggaagatt acagcgtagc agtttgttgt gttttcttcg
     8941 tttccggttc ccagagcgct tccagctcct caagggtttt acctttggtt tccgggacaa
     9001 atttccacat aaacagtgct gccagaacgc ccatacaacc gtaaatccag taggagaaac
     9061 cgttgtggaa atgggccacc agccaggagt ttttgtccat catcgggaag gtccaggaga
     9121 cgaagtagtt cgccagccac tgggccgcca ccgcgattgc cagcgcttta ccacgaatag
     9181 cattcgggaa gatttccgac agcagtaccc agcataccgg accccaggac atggcaaagg
     9241 cggcaacata gaacagcatc gacagtagcg ccacaatacc cgatgcctga gtgtaaaacg
     9301 cggtaccgag gctaaacata ccgattgcca ttccgagtgc gccgataatt tgcagtggct
     9361 tacgaccaaa tttatccaca gtcataattg ccagtacggt aaaggtgagg ttgataactc
     9421 cgacaataat ggtctgcaac agcgcgatat ccgtgctggc ccccagcgtt ttgaacactt
     9481 ccggcgcgta gtacagcacc acattgatgc cgacaaattg ctggaagatg gagagcataa
     9541 cgccgattac aatcacgccc acgccaaaca tcagcagacg accaccggtt ttgcggccat
     9601 gatccaggga gtgtttaatt tcctgtactg cctgagttgc aagcgtgttg cccataattt
     9661 tgcgcaggat actttccgtc tgttcttgct tgccgcgtga catcagccag cgaggacttt
     9721 ctggcacggt atacagcagc attaagaaca gcagtgcagg gatacattcc gaggcaaaca
     9781 tataacgcct gccgtcagta ttcagccagc tggcatcacc ggaacgggca ataaaatagt
     9841 ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa ctggttaaat gaaaccagtt
     9901 ttccgcgaat atgagctgga gccagttccg caatatacat tggtgaaagc attgaggcta
     9961 aaccaacgcc aataccgcca ataatacgat aaataacaaa ttccgggaca taacctgcca
    10021 gataaatagg tactgtgttg tccgggttta tagaggtaaa accaagttct ggccaggcag
    10081 aacctacacc agaaataaaa aacaggacag cagaaatctt aagtgaatca cgacggccga
    10141 agcggttact gcaataacca ccgagggcac cgccgatgat gcaaccaatc agagcgctgg
    10201 ctacgcaaaa tcctaacagg gagttggcag cggattcact taagttttgt ggagcaacaa
    10261 agacggtatt gagtgactca acagtaccgg aaataacggc ggtgtcgtag ccaaataata
    10321 aaccacctaa tgtagcgact aaggtaatcg aaaatatata actggaatta tactgggtat
    10381 tcattcagac ctgccttaga ccattctgat cttttatcag tgcattgatg ttggaattgt
    10441 caggcagtta aggcaattat cattttttac aatgcgttta cgtaatgtct tttctgtgat
    10501 cttaattgtg ataattatca aaaattgatg aaaacaatac tattgccgtg actcagagca
    10561 cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa aaataataag ttctatgatg
    10621 ggaatattaa ccggcgaacg attcagattg cagacgaaag aaaaa
//
LOCUS       DAJVRP010000121        10628 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVRP010000121 DAJVRP010000000
VERSION     DAJVRP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.121"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            50..1540
                     /locus_tag="OQN12_003476"
     CDS             50..1540
                     /locus_tag="OQN12_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1655678.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1592..2749
                     /locus_tag="OQN12_003477"
     CDS             1592..2749
                     /locus_tag="OQN12_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1655679.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2715..2837)
                     /locus_tag="OQN12_003478"
     CDS             complement(2715..2837)
                     /locus_tag="OQN12_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655680.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3040..4725
                     /locus_tag="OQN12_003479"
                     /pseudo
     CDS             3040..4725
                     /locus_tag="OQN12_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4763..7123
                     /locus_tag="OQN12_003480"
     CDS             4763..7123
                     /locus_tag="OQN12_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1655681.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQETRKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7180..9963
                     /locus_tag="OQN12_003481"
     CDS             7180..9963
                     /locus_tag="OQN12_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1655682.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10324..>10628
                     /locus_tag="OQN12_003482"
     CDS             10324..>10628
                     /locus_tag="OQN12_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1655683.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         3151 a         2342 c         2296 g         2839 t
ORIGIN      
        1 gccgactgaa tacagtacca aaggaaagcc aaatattatc gtactaacca tggatgatct
       61 tggttatgga caacttcctt ttgataaggg atcttttgac ccaaaaacaa tggaaaatcg
      121 tgaagttgtc gatacctaca aaatagggat agataaagcc attgaagctg cacaaaaatc
      181 aacgccgacg ctcctttcat taatggatga aggcgtacgt tttactaacg gctatgtggc
      241 acacggtgtt tccggcccct cccgtgccgc aataatgacc ggtcgagctc ccgcccgctt
      301 tggtgtctat tccaataccg atgctcagga tggtattccg ctaacagaaa ctttcttgcc
      361 tgaattattc cagaatcatg gttattacac tgcagcagta ggtaaatggc acttgtcaaa
      421 aatcagtaat gtgccggtac cggaagataa acaaacgcgt gactatcatg acaacttcac
      481 cacattttct gcggaagaat ggcaacctca aaaccgtggc tttgattact ttatgggatt
      541 ccacgctgca ggaacggcat attacaactc cccttcactg ttcaaaaatc gtgaacgtgt
      601 ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag gcaattggcg ttgttgatcg
      661 tgccaaaaca cttgaccagc cttttatgct ttacctggct tataatgctc cgcacctacc
      721 aaatgataat cctgcaccgg atcaatatca gaagcaattt aataccggta gtcaaacagc
      781 agataactac tacgcttccg tttattctgt tgatcagggt gtaaaacgca ttctcgaaca
      841 actgaagaaa aacggacagt atgacaatac aattattctc tttacctccg ataatggtgc
      901 ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga gtcagaccta
      961 tcctggcggt actcacaccc caatgtttat gtggtggaaa ggaaaacttc aacccggtaa
     1021 ttatgacaag ctgatttccg caatggattt ctacccaaca gctcttgatg cagccgatat
     1081 cagcattcca aaagacctta agctggatgg tgttcccttg ctgccctggt tgcaagataa
     1141 gaaacaaggc gagccacata aaaatctgac ctggataacc tcttattctc actggtttga
     1201 tgaggaaaat attccattct gggataatta ccacaaattt gttcgccatc agtcagacga
     1261 ttacccgcat aaccccaaca ctgaggactt aagccaattc tcttatacgg tgagaaataa
     1321 cgattattcg cttgtctata cagtagaaaa caatcagtta ggtctctaca aactgacgga
     1381 tctacagcaa aaagataacc ttgccgccgc caatccgcag gtcgttaaag agatgcaagg
     1441 cgtggtaaga gagtttatcg acagcagcca gccaccgctt agcgaggtaa atcaggagaa
     1501 gtttaacaat atcaagaaag cactaagcga agcgaaataa ctaaaccttc atgcggcgga
     1561 tttttccgcc gccttattga gcgagatagc gatgcacgtt acagccaagc cctccagttt
     1621 tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc agtttactca
     1681 tgaaaaatgg atggatgaca gcactctgaa agagttcatc aaacaatata tcgaagcgtc
     1741 tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt
     1801 tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatgc
     1861 attacaaacg aatggcattt tattgaataa tgaatggtgt gcctttctca aagaacatga
     1921 atttctggtt ggtatctcga tcgatggccc ccaggagtta catgaccgtt acagacgcag
     1981 taattcaggt aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata
     2041 tcaagtagag tttaatacgt taaccgtcat taataacgtt aatgtccatt atcctcttga
     2101 ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctaga
     2161 aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga
     2221 tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta tgcaatgggt
     2281 taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca gccgtttttt
     2341 ggggaatggg catacaagtt gtattttcca ggagtcctgc aaggataatc tggttgttga
     2401 aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca aaattggaaa
     2461 cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg
     2521 gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgtcctaa
     2581 gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag gttataaaat
     2641 ccttttttca accatggtac cttatatgaa cgccatggtt gagttagcta agaacagagt
     2701 accgctttac cacattatgg atgttgcaaa acaaatggag aataattaat aacgctctct
     2761 tttaactcca ttcttagcct ccttcttatg accttaacaa ggcacccgac ataaattcct
     2821 gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ctggcttacg tggtatgcgt
     2881 cagggcatta gcaataccct tgattatcaa tagcataacc ataaccactg aaaattaaaa
     2941 atttctatat atttcatatt gttaaatgat gcttaaataa atttcatcag taaatcattg
     3001 ccatagataa tcattggatg gcacaatcaa ttttcgctaa tgataaccat tcccattacg
     3061 ttttgtatgt tgatagcaaa gtatctctgt ctgctgaagc ccttttggct gcgtaagaat
     3121 aataaaactt cagtgttgtt aatcattatt atcctcgcaa tgatccttgg cgtggttaaa
     3181 attcaggtct ggttaaacga ctggaataat gattttttca atgcgctaag ccagaaagaa
     3241 accgataagc tttggcagct cgttctttgg ttccctgccc tgctggggat ctttgtatta
     3301 atatccgtaa acaaaacatg gttaattaaa ctactgacta ttcgctggcg cgaatggtta
     3361 acagattact accttaatcg ctggtttgca gataaaaact actacttcac gcagatctac
     3421 ggcgagcata aaaatacaga taaccctgac caacgtatcg ctgaagacat tctcttactg
     3481 atcaacaaaa cgctcagtct ttcatttggc ttcatccagt cacttagcat gctgatcacc
     3541 tttaccgtta ttctttggca aagcgcgggt acgctctcat tcactgttgg tggaacagaa
     3601 tggaatatcc aggggtatat ggtctatacc gtcgtgctca ttgtgatcgg tggaacttta
     3661 tttacccata aggtgggtaa acgtattcgt ccgcttaatg tggaaaaaca acgtagcgaa
     3721 gcgacatttc gaactaatct tgtgcagcat aataagcagg cagaactgat tgcgctaagc
     3781 aatgctgagt ctctacaacg ccaggagttg cgcgacaatt ttcataccat taaagagaac
     3841 tggcatcgtt taatgaatcg tcagcggtgg cttgattact ggcaaaatat ttattcgcgc
     3901 tcgcttagcg ttcttcccta ctttctgtta ttgccgcagt ttattagcgg gcagatcaat
     3961 ctgggcggac tgatgaaatc gcgccaggca tttatgctag tatcgaacaa tttaagctgg
     4021 tttatttata aatatgacga acttgctgaa ctggctgcgg ttatcgatcg cttgtatgag
     4081 ttccatcaac tcactgaata gcgccctacg aataagccta aaaattgcca acatgcggta
     4141 caagtggcta atgcgagtat tcgtacgcct gataataaga tcatattaga gaacttgaac
     4201 tttcatgttt cgccaggcaa atggctatta ctgaaaggct actctggtgc gggaaaaacc
     4261 acactgctta aaacattatc ccactgctgg ccgtggttta aaggtgatat ttcttctcct
     4321 gctgacagtt ggtatgtgtc acaaacaccg ttaatcaaaa gcggcttact gaaagagatt
     4381 atttgtaaag cacttcccct gcccgtagac gataaatcgt tgagcgaagt actgcatcag
     4441 gttggtctgg gaaaattggc tgcgcgtatt catgaccacg atcgctgggg agatattctt
     4501 tccagcggcg aaaaacaacg tatcgccctg gcacgattaa ttttacgacg tccgaaatgg
     4561 atatttcttg acgaaactac ctctcatctt gaggaacaag aggctatacg cttactgcgt
     4621 ttagtgcgtg aaaaactacc cacaagcggc gtcattatgg ttacacatca acccggtgtc
     4681 tggaacctgg ctgatgatat ttgtgacatt agcacggttt tataacaaca ataaaagccc
     4741 tgctgtacgg agatataaat aaatgaagcg agttcttatt cctggcgtca ttttatgtgg
     4801 cgctgatgtg gcgcaggccg tcgatgacaa aaacatgtac atgtattttt ttgaagagat
     4861 gacggtctat gctcctgtcc ctgtacccgt aaacggcaac acgcattaca ccagtgaaag
     4921 catcgagcgt ttaccgaccg ggaatggcaa tatcagcgat ctgctgagaa ccaaccctgc
     4981 ggtacgcatg gattcaacgc aaagtacctc gttgaaccag ggagatattc gccctgagaa
     5041 aatctctatt cacggtgcgt cgccctacca gaatgcctat ttgattgacg gtattagtgc
     5101 aactaataac ctgaacccag cgaatgagtc cgatgccagt agtgcaacca atattagcgg
     5161 gatgtcacag gggtattatc ttgatgtcag cttactggac aatgtgacgc tttatgacag
     5221 ttttgtgccg gttgaatttg gtcgcttcaa tggcggggta attgatgcaa agatcaaacg
     5281 cttcaacgct gatgatagca aggtgaaatt gggttatcgc actacgcgtt tggactggtt
     5341 aacatcgcat atcgatgaga ataacaagag cgcatttaat caaggttctt caggaagtac
     5401 ctatttctct ccagatttta aaaagaactt ttataccttg tcgtttaatc aggagctggc
     5461 tgataacttt ggcgttaccg ccggtttatc gcgccgccag tctgatatca cccgcgcgga
     5521 ttatgtttcg aatgacggca ttgtcgccgg tcgggcacag tataaaaacg ttatcgatac
     5581 tgcattgagc aaatttacct ggtttgccag cgaccgcttt acccacgatt taaccttaaa
     5641 atataccggc tccagccgtg attataatac cagcaccttc ccgcagtctg atcgcgaaat
     5701 gggtaataaa tcctatggtc tggcatggga tatggatacg caactcgcat gggccaaact
     5761 acgtaccacc gttggttggg atcatattag tgattatacc cgtcacgatc atgacatctg
     5821 gtacaccgaa ctttcatgta catatggtga tattacaggg cgttgtaccc gtggcggatt
     5881 aggacacatt tcccaggctg tagataatta caccttcaaa acacgcctgg actggcaaaa
     5941 attcgccgtg ggtgatgttt cgcatcaacc ctacttcggc gcggaataca tctattccga
     6001 tgcatggact gaacgccata accagtctga atcctatgtg attaatgctg caggaaagaa
     6061 aactaaccat accatttacc ataaaggtaa aggcagcctg ggaattgaca actacacgct
     6121 gtatatggcg gatcacatta gctggcggaa tgtgtcgtta atgcccggtg tgcgttatga
     6181 ctatgacaac tatctgtcaa accacaatat ctccccgcgc tttatgacgg aatgggatat
     6241 ttttgctgat caaacctcaa tgattaccgc cggttataac cgttactatg gcgggaatat
     6301 tcttgatatg ggattacgtg atatccgcaa tagctggacg gaatcggtat caggtaataa
     6361 aaccctgacg cgttatcaga atttgaaaac gccttataac gatgaactgg caatgggatt
     6421 gcagcaaaaa atcgataaga acgttattgc acgcgcaagt gaagcgcatg atcaaatcag
     6481 caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg
     6541 caaaaccaaa acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg
     6601 ccaggtagat attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc
     6661 gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa
     6721 tctggtctct tacgatagcg ttccagtggc agattttaat aacccattaa agatctcctt
     6781 aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca
     6841 agaaacgcgt aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata
     6901 ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg
     6961 cttgtcctgg acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct
     7021 caatgtactg gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc
     7081 cagtggccgt actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt
     7141 aaccgggtaa attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta
     7201 ctgacgttag tggcaactct gttgctcccc gggcggctga ttgccgccgc cttaccgcag
     7261 gatgaaaagt taattaccgg gcaactggac aatgacttgc gatatatgat ttatccgcat
     7321 gctcatccaa aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa
     7381 gaagacaatg agctcggcgt ggctcatttt gtagaacata tgatgtttaa cggcacaaaa
     7441 acatggccgg gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtcgc
     7501 gatgttaatg cctatatcag ctatgacgaa acggtgtatc aggtgagttt gccgactacg
     7561 cagaaacaaa atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc
     7621 tttgaaaaac tcgaagtaga cgctgaacgt ggcgtaatta ctgaggaatg gcgtgcccat
     7681 caggatgcga aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt
     7741 aatttagacc gtgaacctat cggcctgatg gatactgtcg ccacggtcac gccggcacaa
     7801 ttgcgccaat tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc
     7861 gatatcgaca gtaaagaagc gctggcgctg ataaaggata atttaagtaa gcttccggct
     7921 aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat
     7981 atcattaatg ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg
     8041 gtacaagtga acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag
     8101 ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc
     8161 ggcactgcgc gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat
     8221 gcacgagacg ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt
     8281 gatcagcatg gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg
     8341 ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg
     8401 gcatccagct cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg
     8461 aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga
     8521 aagaaccagg acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca
     8581 ttgtctcctg cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct
     8641 tacatcttcc caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt
     8701 agcagcaaag aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag
     8761 gttattctgg caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat
     8821 aaaggcgatt taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca
     8881 gttagcggaa gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa
     8941 aactcggtaa ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg
     9001 cggactaata accctgaacc tggtttccag ttgattaacc agcgaatcac ccacagcacg
     9061 attaacgaca atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc
     9121 gaccagcgtc cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac
     9181 cgcacgaaat tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct
     9241 gccgatcgcc agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc
     9301 gcagaagaca aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat
     9361 tcgccattag ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta
     9421 aaagaacaaa atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca
     9481 cctgttaatc tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat
     9541 ctacgcgtta atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg
     9601 gttgatcctc aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga
     9661 catgatgaac agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc
     9721 agtgagcaag aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag
     9781 cgtagcgttc aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct
     9841 gcagcatgga ctgagcagga gcaattgttg aaacaaatga cggtagagaa tgttaacact
     9901 gccgttaaac aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa
     9961 taataacagc cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat
    10021 gtaataattt tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg
    10081 cctgattttt acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa
    10141 cacctttcca ccacccaaga attctctatt acattgtttt taatgaaaaa atatcattcc
    10201 aacaatatct cacgataaat aacattagga ttttgttatt taaacacgag tcctttgcac
    10261 ttgcttactt tatcgataaa tcctactttt ttaatgcgat ccaatcattt taaggagttt
    10321 aaaatggata agaagcaagt aacggattta aggtcggaac tactcgattc acgttttggt
    10381 gcgaagtcta tttccactat cgcagaatca aaacgttttc cgctgcacga aatgcgcgac
    10441 gatgtcgcat ttcagattat caatgatgaa ttatatcttg atggcaacgc tcgtcagaac
    10501 ctggccactt tctgccagac ctgggacgac gaaaacgtcc acaagttgat ggatttatcc
    10561 attaacaaaa actggatcga caaagaagaa tatccgcaat ccgcagccat cgacctgcgt
    10621 tgcgtaaa
//
LOCUS       DAJVRP010000122         9951 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVRP010000122 DAJVRP010000000
VERSION     DAJVRP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.122"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OQN12_003483"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OQN12_003483"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1655684.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OQN12_003484"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OQN12_003484"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1655685.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OQN12_003485"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OQN12_003485"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1655686.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OQN12_003486"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OQN12_003486"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS1655687.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="OQN12_003487"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="OQN12_003487"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCS1655688.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="OQN12_003488"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="OQN12_003488"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCS1655689.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="OQN12_003489"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="OQN12_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="OQN12_003490"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="OQN12_003490"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1655690.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="OQN12_003491"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="OQN12_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1655691.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..>9951)
                     /locus_tag="OQN12_003492"
     CDS             complement(8974..>9951)
                     /locus_tag="OQN12_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1655692.1"
                     /translation="IACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2349 a         2677 c         2506 g         2419 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa t
//
LOCUS       DAJVRP010000123         9928 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVRP010000123 DAJVRP010000000
VERSION     DAJVRP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.123"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..339
                     /locus_tag="OQN12_003493"
     CDS             <1..339
                     /locus_tag="OQN12_003493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655693.1"
                     /translation="LSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            527..1642
                     /locus_tag="OQN12_003494"
     CDS             527..1642
                     /locus_tag="OQN12_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655694.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2321..2539
                     /locus_tag="OQN12_003495"
     CDS             2321..2539
                     /locus_tag="OQN12_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCS1655695.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2757..2999
                     /locus_tag="OQN12_003496"
     CDS             2757..2999
                     /locus_tag="OQN12_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655696.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="OQN12_003497"
     CDS             complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="OQN12_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1655697.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="OQN12_003498"
     CDS             complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="OQN12_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1655698.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="OQN12_003499"
     CDS             complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="OQN12_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1655699.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="OQN12_003500"
     CDS             complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="OQN12_003500"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8265..8267),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1655700.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9046..9879
                     /gene="fdhD"
                     /locus_tag="OQN12_003501"
     CDS             9046..9879
                     /gene="fdhD"
                     /locus_tag="OQN12_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1655701.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2396 c         2543 g         2499 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
      541 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
      601 tccgtcgatc accaataaat actcagcaaa agatgtaggt ccagatatac ttgcattgcc
      661 cgtcaacgta tatcatgcgg attttaaaat taaaatcaat aatgcagtgg agaaggcttc
      721 atcaattgtg ttatgtgctg tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg
      781 gataatagga aatgtgccaa aaaataataa accaaaaaca attatttttg atttgcgttt
      841 tcttagtgca attaagcaga aagttttttt gggcagtgaa aaaccgttgt ttgtaaagca
      901 taccgcaatg atgagggagg catgtaagga attaccgcag tcagtggaat atgttccttt
      961 agcctgtgaa gcgcatagcc atcgcagcgt cgcccttgcc aaaaccgtgg ctgcgcccat
     1021 catggcgaca ggtgtgggct tggcattaat gtatgcaaca tcatcaggac cgtattatgc
     1081 gcaaagtctt tctggttcgc cggatatcga gatgataaaa aatcgcatgg ctgaattgtt
     1141 taaccagtcg gatatttctg tgcgcaacaa atatagcccg gcttttgata aatggaatga
     1201 actggtagat aaattaaatc acgaaagaaa tacagttccg tttgcgtgtt tgaccaccat
     1261 tttagcgacg gcgataaatg aaactcttga gggggatacg aatgttgccg ttgtgatggg
     1321 gtgtaaatct gcgaaagatc gcacgatatc cattgtcctt ggcaactcga tgttgcaaac
     1381 actccttgaa aaacgcctcg ccgatagtgg agaaatagaa aatctttttg atcaacaggg
     1441 atattttagt tgcgattcat taacacccga agaattaatg atgttaaaag acctttttga
     1501 tattcgagtt cttcatgtct ctaacaaatt caatgttgga ttgcaaggta atatcaacac
     1561 tgatgtttta caagattcgt tttttaaaaa tgtcgatttc attcatgaat ataactcgta
     1621 taccgtaggg atgggagtct gacgagtaaa actcattagg attatcagaa ttcggacaat
     1681 gcattgcaaa aatgacatac aaagagagat gtgtgcaggt acttagccgc gaggggcatg
     1741 gcgaagccat acagaaagta ccagtattca gaactccagt gaaagcggca tagtggccgc
     1801 ctgctcagga gttttgctat gacccgtaat cgtgctgaac gtcgccaccc tatgatgcga
     1861 ttaaaaaaac tcatcgtcgc gctgatgatt gacgtcaatg ctatcggatg atgccggggg
     1921 tgttccggaa ggaattcctt gtttcccgaa ctgttaccaa caaaattata acgatgagca
     1981 agtaaagacc ggttggtggc agtactgacc ggtctgtgat gaaagattaa tttcacctta
     2041 aatcctggtg ctgttaggat gctgacgttt ttcggctaca ccattcagat tatgcaaata
     2101 aatatccctg aatataatat attcatagca cccataactc atcctgggcg ataactaaac
     2161 catttttgag taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg
     2221 caaaaacaaa atgacatgca aaaatccgca tttttttaaa tgcttttccg gcgtaatttt
     2281 cgcacataca ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt
     2341 tcttcattta tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt
     2401 ttcacgatct gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc
     2461 ggtgcgtaaa gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg
     2521 taaggagcat aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt
     2581 attctggtat taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc
     2641 gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga
     2701 ttcggcaagg gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga
     2761 actctctggc aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta
     2821 agcaacttga tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag
     2881 cggtagatca atacctgata aatcaatcgc aaacagcaag aaccagtgtt cctggcatct
     2941 ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat
     3001 gggaaaaaat gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg
     3061 cgtgaacgcc ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt
     3121 aggcctgata agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata
     3181 accttatcga tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga
     3241 atgggtaacg cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg
     3301 acctgcgcca cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt
     3361 taatggaact acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga
     3421 ggtcatctgc cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag
     3481 tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt
     3541 aatgcagttt gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg
     3601 tttcacacag gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc
     3661 tggacaccgg catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc
     3721 gggctttgcc ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga
     3781 taaacggcgc tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca
     3841 tatcttccag ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc
     3901 cgctcatttc aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat
     3961 cacgcggcag aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc
     4021 gcgcggtcag atccatctcc agcggatggt cgtacagcac cactttctgg gcgtgggcga
     4081 taagcgcagc aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac
     4141 gctcggcgcg gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat
     4201 ccgccgtacg tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc
     4261 agctttcttt tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc
     4321 ccaggcgctg gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc
     4381 ggcgtagata tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat
     4441 taacgcgaat cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat
     4501 cacgccgctc accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg
     4561 accgaagtta taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt
     4621 cttcgcccaa aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat
     4681 gatgatgaac gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg
     4741 cggtgtgccc atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct
     4801 caccgccgcc aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt
     4861 gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga
     4921 ttattctctt cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc
     4981 gcaaaggtag ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc
     5041 acggtttcgc tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca
     5101 tggtgcagca cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca
     5161 ttgtcgtaac cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac
     5221 tctttcgtgc cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc
     5281 ccaaccacca cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg
     5341 aggcgcggaa tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg
     5401 gactggaagt cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct
     5461 ttcaggcagc ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt
     5521 ttgtcgttct gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca
     5581 ttggggttgt cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct
     5641 gaacaagcca cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc
     5701 acttcttcct ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga
     5761 cgaatgatat cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc
     5821 acaaggaagg acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa
     5881 gtattggcaa taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg
     5941 atggtatcga tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct
     6001 ttggctttga tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca
     6061 agtttattcg ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc
     6121 aacagcgcgt gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca
     6181 tacgggaact tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagct
     6241 gggttcgaga taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg
     6301 tagtgttccg ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct
     6361 tcctgctgca tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga
     6421 atatcccagc cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac
     6481 gggttaccct gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat
     6541 gcccatgccc agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc
     6601 atctggttgc cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta
     6661 ccgtcatcgc gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca
     6721 ccggttgccg gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct
     6781 tccgacttcg gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg
     6841 ttcgcgccac cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc
     6901 tcaccgtcag tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag
     6961 cgacctgagt taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac
     7021 acttcggtct ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag
     7081 ttagaggttt cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa
     7141 ccgatcactt tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag
     7201 ccattgacct tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc
     7261 cacttcggca accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag
     7321 gccttcatca tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc
     7381 agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt
     7441 ggcagcgtca tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga
     7501 atattggagt gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc
     7561 agcagctgga tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag
     7621 ccgagggcat acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg
     7681 cagactttca ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg
     7741 tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta
     7801 tcgcgtttgg cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg
     7861 cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg
     7921 atcaggctgg cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc
     7981 aggtacagca atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag
     8041 tagtcagcta ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg
     8101 ttgtgaattt tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta
     8161 ccgcccatca ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca
     8221 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg
     8281 ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg
     8341 ttactcgacg cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct
     8401 tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga
     8461 tcaaacgcct cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc
     8521 ggaaacttca gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa
     8581 agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg
     8641 gcgttttttg ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag
     8701 gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt
     8761 gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag
     8821 atcttaaaga actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac
     8881 gtcccattta cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa
     8941 ggggccgcaa aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag
     9001 aataccataa tgttggtgtg tatgttctta tctggttaag agaaagtgaa aaaaacacag
     9061 cgaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg
     9121 cgtgatgatt tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg
     9181 gtctacaacg gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt
     9241 gcgctcggtt tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg
     9301 gatgtcgttc cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt
     9361 atggggttga aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc
     9421 gtggagcaac ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt
     9481 gatctcaaca aactggatga tgcattacgt catctcaacg attttcagcc agtggggcga
     9541 ctgactggtt gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg
     9601 catgaagacg tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa
     9661 ggggaaagct ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg
     9721 caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt
     9781 gctgtagaag tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg
     9841 gcaacggttt atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat
     9901 atattaaatt gcaggactta ttcatttc
//
LOCUS       DAJVRP010000124         9895 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVRP010000124 DAJVRP010000000
VERSION     DAJVRP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.124"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(150..890)
                     /gene="pflA"
                     /locus_tag="OQN12_003502"
     CDS             complement(150..890)
                     /gene="pflA"
                     /locus_tag="OQN12_003502"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS1655702.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OQN12_003503"
     CDS             complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OQN12_003503"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1655703.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OQN12_003504"
     CDS             complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OQN12_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1655704.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4682..6442)
                     /locus_tag="OQN12_003505"
     CDS             complement(4682..6442)
                     /locus_tag="OQN12_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCS1655705.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6572..7198
                     /locus_tag="OQN12_003506"
                     /pseudo
     CDS             6572..7198
                     /locus_tag="OQN12_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7397..8485
                     /gene="serC"
                     /locus_tag="OQN12_003507"
     CDS             7397..8485
                     /gene="serC"
                     /locus_tag="OQN12_003507"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1655706.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8556..9839
                     /gene="aroA"
                     /locus_tag="OQN12_003508"
     CDS             8556..9839
                     /gene="aroA"
                     /locus_tag="OQN12_003508"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1655707.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2519 a         2367 c         2477 g         2532 t
ORIGIN      
        1 aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat atggtagatg
       61 cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa aaagcggcaa
      121 ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc gtactgctca
      181 agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc atcgagtttg
      241 tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg gagaagttcg
      301 attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc cgaatcgtca
      361 tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt tttgttcgcc
      421 agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt ttggtggatc
      481 tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag cagttcatca
      541 atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg aatgccttct
      601 tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc ttcaccgccg
      661 gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac cacttccttc
      721 atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc gcggttatga
      781 caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat acctgggccg
      841 tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat tgcggtgttt
      901 ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct
      961 gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt gctaaaaaag
     1021 gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta
     1081 attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg
     1141 agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat ttttccgggt
     1201 tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg tgctgaccac
     1261 cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg ttggtcttac
     1321 gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag gagataccat
     1381 ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca gcacctttct
     1441 ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt
     1501 ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc agaacagact
     1561 gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg aaacgttcta
     1621 ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc gggtattcgc
     1681 cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta actttcgcat
     1741 atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca cacgccatgg
     1801 tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt
     1861 gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag tccatgaagt
     1921 gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc ggttcagact
     1981 tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg tacagcatgg
     2041 ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg atcatcgggc
     2101 ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt
     2161 tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc ttgaagttca
     2221 gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc cccatagtgt
     2281 acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca tcgaggccca
     2341 taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca tcgtattccg
     2401 gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc atttcctgcg
     2461 cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca tccaggaagg
     2521 tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca gccaggtagc
     2581 cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag atgtcgtagc
     2641 cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca gcgatttctt
     2701 cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc tgtagagagg
     2761 tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca acgcgacggt
     2821 agtcaccgat gatacggcca cggccataag catctggcag accggtcaga acaccggatt
     2881 tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg tgagttttac
     2941 ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac gctttgcagg
     3001 aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc ggagcttcag
     3061 tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg tgagaggtga
     3121 tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt tccagtttaa
     3181 caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca gccaggaagg
     3241 actcgtcacc ctcgtacggc gtgtagtttt tctgaatgaa gtcacggacg tttacttcat
     3301 tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca ttaagctcgg
     3361 acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc gacaaccatt
     3421 aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc aacaaaccac
     3481 cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag ttcatcacgg
     3541 tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc ggagatgcga
     3601 agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg tgctcaaaac
     3661 cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc atcaggctgc
     3721 ggccagaata actcatccat actgccagac ataccatcag gtttgccagg ataccaagac
     3781 agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg acgtttagtc
     3841 cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt acaaacagca
     3901 gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac tgacaccagg
     3961 tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag agatcggctc
     4021 cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc agttttgcca
     4081 tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg aatgcgattg
     4141 agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt ttcgttgctt
     4201 tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa agatcaaaag
     4261 ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat actaacaaag
     4321 cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc ccgtaactcg
     4381 catggttttt atgcaaatac ggagtaacta tttgattatc caaataaaaa taaattttaa
     4441 aaattaacaa atgagttgaa ttttttccgc atcctccgct aaaacagtta attaaaaggg
     4501 agcatcaggc gaataaagta acaatatcga tcgtatttat taaatacaaa ttaccgatat
     4561 ttaaccttat aattacaatt attttattaa tgcaaatata tgtaaagcgg ggcattaaaa
     4621 aaacgccccg taatataact cagactaatc attaagccta cattgcgtag gctatttgat
     4681 tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacaacg
     4741 actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg taaaacgccg
     4801 cttcgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac atgcgaacaa
     4861 acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat aacaacgttt
     4921 gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta aactccatcg
     4981 tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt
     5041 ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc agacccaaca
     5101 gctcacgcac gcgggtaaag tcatcaaaat cttcttcatc cagttgctcg atgaggttca
     5161 ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca cgtaaatggc
     5221 tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg gacatgccag
     5281 gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata taaacttctt
     5341 tatcttcttt gttgaagata gccatcagcg tagcaaactc ttcttccgtg gtgccggaga
     5401 aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac
     5461 cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct tcatcatcga
     5521 aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc agctcggtca
     5581 cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca aagcaggtgc
     5641 cgtttgcagg attaaacagt accacgcaaa tcaccggata ctggccgcca agggaaccat
     5701 catatgcgaa gatcgggaaa ccttctgttt ccagtgtttc gatcgcttca accaccgctg
     5761 ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca gcaataatgc
     5821 ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttgcggg
     5881 tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc atcggaatat
     5941 aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca cgatcttcgt
     6001 taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga tcgtaaaatg
     6061 cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcgctc agtgggaacc
     6121 atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc agccagaaat
     6181 cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca gaagccagcg
     6241 ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa
     6301 tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca atctgaaagc
     6361 cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg gcatctttgc
     6421 cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata
     6481 cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt gactatgctt
     6541 ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc accgtatggc
     6601 atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc tttatacctt
     6661 tgtactggtt ttttgttcct caaaatgacc ggaagacgcg gtgtgcgaca gatgtcgctg
     6721 tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc gttttatgat
     6781 gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt ataccgcctg
     6841 gtaatatgat ggcgagctgg cctggtcgaa actcaataac tccaacatga cggaatttga
     6901 gttctttatg gagctacgat tgcgtggcgt ggagcagctg gggcaggtac gtctggcgat
     6961 tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa gatgacaagg tgaaaccggg
     7021 tttacttatt ttacccagtg attgtacgca gcgttacaaa gtggtgccgg agtcggcgga
     7081 ctatgcctgc attcgttgta gtgaaatcat tcatatgaac gcgggggaaa aacaattatg
     7141 tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg gcaaaacggg tgacctgaca
     7201 gtaaaaacat cggctttttg ctaataatcc gagagattct tttgtgtgat gcaagccaca
     7261 tttttgccct caacggtttt actcattgcg atgtgtgtca ctgaatgata aaaccgatag
     7321 ccacaggaat aatgtattac ctgtggtcgc aatcgattga ccgcgggtta atagcaacgc
     7381 aacgtggtga ggggaaatgg ctcaaatctt caattttagt tctggtccgg caatgctacc
     7441 ggcagaggtg cttaaacagg ctcaacagga actgcgcgac tggaacggtc ttggtacgtc
     7501 ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag gttgcagagg aagccgagaa
     7561 ggattttcgc gatcttctta atgtcccctc caactacaag gtattattct gccatggcgg
     7621 tggtcgcggt cagtttgctg cggtaccgct gaatattctc ggtgataaaa ccaccgcaga
     7681 ttatgttgat gccggttact gggcggcaag tgccattaaa gaagcgaaaa aatactgcac
     7741 gcctaatgtc tttgacgcca aagtgactgt tgatggtctg cgcgcggtta agccaatgcg
     7801 tgaatggcaa ctctctgata atgcggctta tatgcattac tgcccgaatg aaaccatcga
     7861 cggtatcgcc atcgacgaga cgccagactt cggcaaagat gtggtggtcg ccgccgactt
     7921 ctcttcaacc attctttccc gtccgattga cgtcagccgt tatggtgtaa tttacgctgg
     7981 cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc atcgttcgtg aagatttgct
     8041 gggcaaagcg aatatcgcgt gtccgtcgat tctggattat tccatcctca acgataacgg
     8101 ctccatgttt aacacgccgc cgacatttgc ctggtatcta tctggtctgg tctttaaatg
     8161 gctgaaagcg aacggcggtg tagctgaaat ggataaaatc aatcagcaaa aagcagaact
     8221 gctatatggg gtgattgata acagcgattt ctaccgcaat gacgtggcga aagctaaccg
     8281 ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg cttgacaaat tgttccttga
     8341 agagtctttt gctgcaggcc ttcatgcact gaaaggacac cgtgtggtcg gcggaatgcg
     8401 cgcgtctatt tataatgcca tgccgctgga aggcgttaaa gcgctgacag acttcatggt
     8461 tgagttcgaa cgccgtcacg gttaatgccg aaattttgct taatccccac agccagcctg
     8521 tggggttttt atttctgttg tagagagttg agttcatgga atccctgacg ttacaaccca
     8581 tcgctcgtgt cgatggcact attaatctgc ccggttccaa gagcgtttct aaccgcgctt
     8641 tattgcttgc ggcattagca cacggcaaaa cagtattaac caatctgctg gatagcgacg
     8701 acgtgcgcca tatgctgaat gcattaacag cgttagggtt aagctatacg ctttcagccg
     8761 atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt acacgcagaa ggtgccctgg
     8821 agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct ggcggcagct ctttgtctgg
     8881 atagcaatga tattgtgctg accggtgagc cgcgtatgaa agagcgcccg attggtcatc
     8941 tggtggatgc gctgcgcctg ggcggggcga agatcactta cctggaacaa gaaaattatc
     9001 cgccgttgcg tttacagggc ggctttactg gcggcaacgt tgacgttgat ggctccgttt
     9061 ccagccaatt cctcaccgca ctgttaatga ctgcgcctct tgcgccggaa gatacggtga
     9121 ttcgtattaa aggcgatctg gtttctaaac cttatatcga catcacactc aatctgatga
     9181 agacgtttgg tgttgaaatt gaaaatcagc actatcaaca atttgtcgta aaaggcgggc
     9241 agtcttatca gtctccgggt acttatttgg tcgaaggcga tgcatcttcg gcttcttact
     9301 tcctggcagc agcagcaatc aaaggcggca ctgtaaaagt gaccggtatt ggacgtaaca
     9361 gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa aatgggcgcg accatttgct
     9421 ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa cgctattgat atggatatga
     9481 accatattcc tgatgcggcg atgaccattg ccacggcggc gttatttgca aaaggcacca
     9541 ccacgctgcg caatatctat aactggcgtg ttaaagagac cgatcgcctg tttgcgatgg
     9601 caacagaact gcgtaaagtc ggcgcggaag tggaagaggg gcacgattac attcgtatca
     9661 ctcctccgga aaaactgaac tttgccgaga tcgcgacata caatgatcac cggatggcga
     9721 tgtgtttctc gctggtggcg ttgtcagata caccagtgac gattcttgat cccaaatgca
     9781 cggccaaaac atttccggat tatttcgagc agctggcgcg gattagccag gcagcctgaa
     9841 tgaacaacgg gcaataaata gccaaatctt tctttatcaa aacgtcggca cattg
//
LOCUS       DAJVRP010000125         9865 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVRP010000125 DAJVRP010000000
VERSION     DAJVRP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.125"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            73..204
                     /locus_tag="OQN12_003509"
     CDS             73..204
                     /locus_tag="OQN12_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCS1655708.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            464..1459
                     /gene="pgl"
                     /locus_tag="OQN12_003510"
     CDS             464..1459
                     /gene="pgl"
                     /locus_tag="OQN12_003510"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1655709.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1500..2453)
                     /locus_tag="OQN12_003511"
                     /pseudo
     CDS             complement(1500..2453)
                     /locus_tag="OQN12_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2637..3689
                     /locus_tag="OQN12_003512"
     CDS             2637..3689
                     /locus_tag="OQN12_003512"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCS1655710.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3765..5198
                     /locus_tag="OQN12_003513"
     CDS             3765..5198
                     /locus_tag="OQN12_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCS1655711.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5381..7561
                     /locus_tag="OQN12_003514"
     CDS             5381..7561
                     /locus_tag="OQN12_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1655712.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7702..8985)
                     /locus_tag="OQN12_003515"
     CDS             complement(7702..8985)
                     /locus_tag="OQN12_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1655713.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9120..9812)
                     /locus_tag="OQN12_003516"
     CDS             complement(9120..9812)
                     /locus_tag="OQN12_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1655714.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAATRA
                     AKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         2396 a         2322 c         2506 g         2641 t
ORIGIN      
        1 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
       61 gggggattga ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
      121 ctacaacgta atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt
      181 tggccagaag ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg
      241 gagagtcata ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg
      301 tgactaatat tttagtaata tgccattcga aatggagtgc taacttattg atttaattga
      361 tgtgcgatat tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta
      421 tatgtatgaa agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta
      481 tatcgccagc cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact
      541 gacgctgaca caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga
      601 caaacgttat ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc
      661 cccggacgat ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca
      721 tatttccacc gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt
      781 gagcgtaacg cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct
      841 ggacggttgc cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt
      901 aaagcaggat cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga
      961 ccctgcggaa gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa
     1021 cgaacaatat gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa
     1081 agatccgcac ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc
     1141 cgacacccgt tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg
     1201 cgaccgtacc gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag
     1261 taaagaaggc ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg
     1321 caagtatctg attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg
     1381 cgagcagggg ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt
     1441 ggtggttaac gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat
     1501 taacttatct cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct
     1561 ttatcgtcag tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc
     1621 acggcatctt tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg
     1681 ataccaataa tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc
     1741 cccagtcctg cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag
     1801 tccagcttcc agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag
     1861 gaacgtacgc caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg
     1921 gccaatggat ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa
     1981 tcaatggatg aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc
     2041 accgattcca tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac
     2101 agctcattaa atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac
     2161 gctaatgtca ccatatcacg atgataagca ttcatatcac gaatattaaa aagcgtttca
     2221 tcgaatttct ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac
     2281 gtaaccttgc gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt
     2341 attcttcgcg ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa
     2401 ctggactctg ccagtatgac aaatgccttc atacttgata attcatgctt cattattact
     2461 ccggaaaatg gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag
     2521 ccaacttaaa ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt
     2581 tgtccttaat gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga
     2641 aaaaaatacc ctgcgtgatg atgcgaggtg gaacctcgag ggccgcgttc ctgttagcgg
     2701 aacatttacc cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg
     2761 gtaacgatct ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca
     2821 ttattagccg ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg
     2881 tccatgagca acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg
     2941 cattcgccat tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc
     3001 gcaacgtcaa tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg
     3061 agtacgaggg tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca
     3121 ctttcctgaa tgccgctgga acgaaaaccg gaaaagtttt cccgactgat aatcagattg
     3181 attattttga cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc
     3241 cggctgaata tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag
     3301 cattattagc ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg
     3361 atgtcagtaa tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg
     3421 caattaatgt gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg
     3481 ctattgctat ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt
     3541 ctgtaggata tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa
     3601 gtaatgaagg tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa
     3661 tattttccgg tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa
     3721 taaaggactt ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt
     3781 tatggaagct aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg
     3841 caggattaag cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc
     3901 tggttatcaa gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg
     3961 tggtggtcgg taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact
     4021 cttcaggtac tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca
     4081 ccggtttagg taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg
     4141 gtctgggtta cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca
     4201 ccgcgcgtgc gggcggcatt gtgttaccga tcatcaacag cgtggcgatg gctttggggt
     4261 ccgaaccgga aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg
     4321 tcaccaaaac caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga
     4381 aaatgatcaa cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg
     4441 gattgccggg catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac
     4501 cagaaattaa aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac
     4561 cgatgaaaat ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga
     4621 ttttcagtaa gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca
     4681 tgctgctgct gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata
     4741 ccttaatctg gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt
     4801 tcgaatggtt agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg
     4861 ctttcttcgt tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg
     4921 cctatatcgt tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt
     4981 taatgttaac cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact
     5041 atggcggcgc ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt
     5101 ggttggtcgg tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt
     5161 ggtggtggaa tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt
     5221 tgcatgtgct gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga
     5281 ttcactcact tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct
     5341 ttctgcgcac ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa
     5401 aggcgtgttt ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat
     5461 taaaaaagaa gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac
     5521 gtccggaaat atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac
     5581 ctttgttggt attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta
     5641 tgtgctgacc aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga
     5701 ccatgttttt ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat
     5761 tgcggtcatc catcaatata tgcgtgagat gatggctagc gggggcaaaa tgatcctcgg
     5821 gtcagacagc cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg
     5881 tgagttggta aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc
     5941 ggtgcatctg accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat
     6001 cattggcgcg gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc
     6061 gggcgttagc gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac
     6121 cacctgttta agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca
     6181 cggtcgcggc caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg
     6241 catcagcgtt gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa
     6301 cgtgtatgaa atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat
     6361 tgagtccgaa cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa
     6421 tggtcgcctg aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa
     6481 cgtcatcgcg gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct
     6541 ggcggtttac ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc
     6601 agatttgatt ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc
     6661 gggcgatacg ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa
     6721 ccgcgaaggc tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg
     6781 ttctatcgct gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg
     6841 ctgggacaac gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca
     6901 gggctttgtg aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg
     6961 gccggaattg ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga
     7021 cgaagtcacc accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa
     7081 tccgattggt ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca
     7141 gattgctggt caggagcata ttgatccgct gcaaactgaa attggcagca tggtctatgc
     7201 ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg
     7261 cggtctggcg aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa
     7321 ctgggggatg ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat
     7381 ttacatccct ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt
     7441 gatccatgaa gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga
     7501 agagcgcgag attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta
     7561 aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc
     7621 atcttatccg acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat
     7681 ccggcaaata gttaatagct cttacttctt cgcctctgca accactttgc tgcccacgcc
     7741 gcggttattg tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc
     7801 gtcgttatca tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc
     7861 ggcccacggt ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg
     7921 accgttggta ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga
     7981 agcattgaaa cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg
     8041 tgcaaacaca tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc
     8101 gaacaccact gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt
     8161 caccagcgta cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac
     8221 aaagaaggtg ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc
     8281 agtacgcagt gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc
     8341 gatggtcaga ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag
     8401 aacaccgata ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt
     8461 acctggcata tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc
     8521 cccatcaagg gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt
     8581 gattccacct ggagcagcag ggacatacac cgttccctga tactccccag gcatcacggc
     8641 aatatactgg cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt
     8701 ggtatgcgtt acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag
     8761 ggttatcggg gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg
     8821 cgcatcgaaa ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc
     8881 agtttgatca gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc
     8941 aaaagccaat gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct
     9001 atgaaataga aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa
     9061 aaagcattgc ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat
     9121 tatttgattt caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg
     9181 gtgtccgact tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag
     9241 agtcttgcag acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga
     9301 cctgatgctt ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca
     9361 cgacgagtag atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt
     9421 tccttcattg atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg
     9481 cctgttttgc tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg
     9541 cataaatcac caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa
     9601 catggtgatg attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat
     9661 ctccttacct ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct
     9721 tcagctattg cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgatgcc
     9781 gccttgccct cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct
     9841 tcaagtggag catcaggcag ccccc
//
LOCUS       DAJVRP010000126         9626 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVRP010000126 DAJVRP010000000
VERSION     DAJVRP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.126"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="OQN12_003517"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="OQN12_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS1655715.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OQN12_003518"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OQN12_003518"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1655716.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="OQN12_003519"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="OQN12_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OQN12_003520"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OQN12_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCS1655717.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OQN12_003521"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OQN12_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1655718.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="OQN12_003522"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="OQN12_003522"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1655719.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="OQN12_003523"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="OQN12_003523"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS1655720.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="OQN12_003524"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="OQN12_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="OQN12_003525"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="OQN12_003525"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1655721.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2489 g         2270 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaatt
//
LOCUS       DAJVRP010000127         9597 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVRP010000127 DAJVRP010000000
VERSION     DAJVRP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.127"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(8..328)
                     /gene="psiF"
                     /locus_tag="OQN12_003526"
     CDS             complement(8..328)
                     /gene="psiF"
                     /locus_tag="OQN12_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1655722.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(447..1862)
                     /gene="phoA"
                     /locus_tag="OQN12_003527"
     CDS             complement(447..1862)
                     /gene="phoA"
                     /locus_tag="OQN12_003527"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1655723.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="OQN12_003528"
     CDS             complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="OQN12_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1655724.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2405..2608)
                     /locus_tag="OQN12_003529"
     CDS             complement(2405..2608)
                     /locus_tag="OQN12_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655725.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2686..3780
                     /gene="ddlA"
                     /locus_tag="OQN12_003530"
     CDS             2686..3780
                     /gene="ddlA"
                     /locus_tag="OQN12_003530"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1655726.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3804..4016)
                     /locus_tag="OQN12_003531"
     CDS             complement(3804..4016)
                     /locus_tag="OQN12_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1655727.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4276..4584
                     /locus_tag="OQN12_003532"
     CDS             4276..4584
                     /locus_tag="OQN12_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1655728.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4643..5737)
                     /gene="yaiW"
                     /locus_tag="OQN12_003533"
     CDS             complement(4643..5737)
                     /gene="yaiW"
                     /locus_tag="OQN12_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1655729.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5750..6970)
                     /gene="sbmA"
                     /locus_tag="OQN12_003534"
     CDS             complement(5750..6970)
                     /gene="sbmA"
                     /locus_tag="OQN12_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1655730.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7322..8479
                     /gene="ampH"
                     /locus_tag="OQN12_003535"
     CDS             7322..8479
                     /gene="ampH"
                     /locus_tag="OQN12_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1655731.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8480..8983)
                     /locus_tag="OQN12_003536"
     CDS             complement(8480..8983)
                     /locus_tag="OQN12_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1655732.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9071..>9597)
                     /locus_tag="OQN12_003537"
     CDS             complement(9071..>9597)
                     /locus_tag="OQN12_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1655733.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2382 a         2448 c         2283 g         2484 t
ORIGIN      
        1 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcacctt
       61 tcagagcttg ttgcgttgct tgattattac attcacgcat cttttgctgc tgtggcgtca
      121 aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac
      181 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt
      241 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa
      301 gcaaggtaac cagtaatgtt attttcatag caccatccct cttcatgttt taaccatgag
      361 cgtttgcgcc cgtgatctgc cattaagtct ggttgctaac agcaaaaaaa ccacccggca
      421 gcgaaaattc actgccgggc gcagttttat ttcagcccca gagcggcttt catggtgtag
      481 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
      541 atacgcaact gactgccggt atgttcttgt gaatcctctt cggaattccc gtaactcatc
      601 accatcactg cgccatcttt agtatttagc gcctgagtga ggcccggcgc tttggtatcc
      661 ggcgcaacaa cctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc
      721 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
      781 atttgcccac aagggttcgc agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc
      841 aggaaaaatc ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgtgcc
      901 agggttggta cactgtcatt acgttgcgga ttaggcgtat aggtgactgc gggcttatcg
      961 atattgccgt ggtacgttgc tttcggccct agccagcgca ctggcatatt gccgtcagca
     1021 aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca
     1081 tcgttcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat
     1141 tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca
     1201 cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc
     1261 ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaagtcac atgtgccacc
     1321 agcgcagcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct
     1381 gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc
     1441 gcgccgttat aggttttgac accggttgac caggcggttg ctgatgcagc cgagtcggtg
     1501 acgtagtccg gtttgccagt ttttttattc agcgcatagt gagtgtattg cccggtaagc
     1561 ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca
     1621 gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta
     1681 tcgctaagag aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg
     1741 ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg
     1801 gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc
     1861 actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc
     1921 ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac aaggatgctt
     1981 caataacttc tttacgtaat cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc
     2041 gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg
     2101 cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag
     2161 ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt
     2221 catgtctgta tttccttatc caaagtatgg agaagttaac attatgtttc acaacttcat
     2281 agtgtctgga agtattattt ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt
     2341 gattaccagc gaaacgtttc tcttatttaa ataattaccc acacaaaata taggctttta
     2401 attatcaacc tttattataa ataaaagcta tgaaatatat tctcttcgtt aacttataat
     2461 taagagaaaa aaactcaccg cattgccatt taagccagga ataaattagc ttcccgttca
     2521 tcatttttgt gatcttgcgc acatctttga gataacgcac tttcttcacc agtacgttgc
     2581 acgctttgtc agcagaagat tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt
     2641 cgtggtaatg tatgcacttt gtgttagacg ggataggttt ttaagatgga aaaactgcgg
     2701 gtaggaatcg tttttggtgg taaatcagcg gaacatgaag tgtctctgca atcggcaaaa
     2761 aacattgtcg atgccattga taaaagtcgc ttcgacgttg tgctgctggg cattgataaa
     2821 caagggcaat ggcacgtcag cgatgccagc aattatctgc taaatgcaga cgatcctgcc
     2881 catattgcgt tgcgcccttc ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat
     2941 cagcttatcg acgcgcaaaa cggtcagccg ttgccgacgg tggatgtcat tttcccgatt
     3001 gtccacggta cgctgggcga agatggttcc ttgcagggaa tgctgcgggt cgccaattta
     3061 ccgtttgtag gttctgatgt tctggcttca gcagcctgta tggataaaga tgtcaccaaa
     3121 cgtctgctac gcgatgccgg gctgaacatt gcgccattta ttaccctgac gcgcgctaat
     3181 cgtcacaaca tcagttttgc cgaagtggag tctaaactgg ggttaccact gtttgtaaaa
     3241 ccggctaatc agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca
     3301 attgccgtcg atctggcgtt cgagttcgat cataaagtga tcgttgagca agggatcaaa
     3361 ggtcgtgaga tcgaatgcgc agttctgggc aacgacaatc cgcaagccag cacctgtggc
     3421 gagatcgtac tcaccagcga tttctatgcc tacgacacca agtacattga cgaagatggc
     3481 gcgaaagtgg tagttccggc agccattgca ccagagatca acgataagat ccgggcgatt
     3541 gccgttcagg cttatcagac gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta
     3601 accccagaga acgaagtggt gatcaacgag atcaacacac tgcctggctt caccaacatc
     3661 agtatgtatc cgaagctgtg gcaagccagc ggtctgggtt acaccgatct gatcacacgt
     3721 ttgattgagc tggcgctgga gcgtcacgct gcggataacg cattgaaaac cacaatgtaa
     3781 tattttaacg cccgataata ttcttattgg gcgttttcag tttcttcatc gcgacgacga
     3841 ataatcaacc ccgccagcca gaaactaatc acccacgtca ccagaccgac ggcataggtc
     3901 tgccaacctt ttgcttcaaa tcccagtacc cccaccacgc cattaagaat gaatataagg
     3961 ccaatggcga acgcatagta gtgccagtca cggcggattt ttacaggcag attcatggca
     4021 gctccgttaa cgaaaaacaa catccttgca tattctgtaa caaccgtctg tggtgtagac
     4081 aacattttcg taattattaa taaatttaac ttaaggttgc cattttgtga ctcacaacga
     4141 agattcacgc ttcgggaata ttcccaacat caatttacca tgattctgat attgacaatc
     4201 taaatgacct gtcagacttg ccataagccg ctggtatcac accagaagag aagcagatga
     4261 cttccggagg tttttatggc tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat
     4321 cgcaatgcct cttcaacgcc tggcaacatt gcctatgcgt tgtttgtgct gttttgcttt
     4381 tgggctgggg cgcaattgct gaacctgtta gtgcatgcgc ccggcgtcta tgagcgttta
     4441 atgcaggtcc aggaaacagg tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt
     4501 ttcgggctga tcccgttttt agcaggctgc ctcatttttg cagtggtggc gctatggctg
     4561 cactggcgac atcgccgcca gtaaaattta cgttgccaga tgcggcctga aacgccttat
     4621 ctggcctgac attcctgttt gatcactgtt tcatacaacg cgcccgccgt tcgtctacgc
     4681 gcttcgcaaa ccaggccgta gtcaggttgc gggtgatttt cgggctttcc agttgaatgc
     4741 caggcaacat ttcgcggggt agtgatttac ccgttttcgc ttcggcaagt tgataaactt
     4801 tcttgtacag cgccgtctcc tcaaaagaga agctgtcacc tttctctaac tgacggcgaa
     4861 tttcgctgtc gctcatcccc agttttcccg ccagtttgcg cgttgccagt tcggttttcc
     4921 tgggttcttt actgtcgtaa cgaattaaat caccgtccag tgccagcttc acgccgctgg
     4981 ccttactgac tgcgttctga aacgcggcat tacggctggc gtaccagcca gcgttaaaat
     5041 cagcaaagcg gtatatcggt gcgctatagc tggcgggata attcagtaaa tggtaagtac
     5101 caaaccacaa cccgccgcgg cgactgaaga cttcctgacg aactgtaccg tccattttcc
     5161 acggataccc tttggtatgc tgttcggcaa aagcaatgct gacctgcatc ggcccaccgg
     5221 tgcgcaccgg attaagcgaa ccaaacagcg tctgccccat tggtaccatg ctgatcagat
     5281 cgtcaaaaat cgcgctcaat tgcttttctg tacgcaccga atctaaccgt tcgctataac
     5341 ttttgccgtt tggcgatttg atttttagcg cggtatgaac cagaaaagca ggaatgtgca
     5401 tccgttcagc acgacggtca atctcttgcc aggcgatctt gcttaagccc ggaacggccg
     5461 gatctgcctg ataatttgac tcctgctgcg ccaccgccag caccgagtag acgttttcca
     5521 gcgtcggtgc cagcccctga ctttcaaagg tggtggcgag atctttcgcc caggcatcgc
     5581 ggtctttcac gctcgcaggc atcttctgcc gcacgacgct cgctacatct atcgcttttt
     5641 cgcccttttt caacggttgc ggagcctgac tactacaccc cgctaaaacc agaaccgcca
     5701 acaatgacag cgaagataga tgattgacgc gcgacattac ttctcctttt tagctcaagg
     5761 tatgggttac ttcctgaatt ttgtcaccat ccagctcatg ttcaaagctg cgcagacgtt
     5821 tgtagataga catcaactca accagtgtgg tccatgagtt aatcaggtac tggaaagcac
     5881 cgcgaacctg accaaaaacg ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg
     5941 caacaattga cggaaacagc aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa
     6001 tgcgggcgat gttgaaatac atatagtgaa aatagaggcg aaaatagttt ttccgtacgg
     6061 cgctaaacag ctcgcgtacc gtaggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa
     6121 ccagctcttt acggtaggcg gcctctactc gctggttttt aaactccagc cccggcagtt
     6181 tgatccctac cactgccagc aatccggtcc ccatcagcga ccagacgata gcggcaatca
     6241 ccagaccata cggaatgtgc ccgataatcg gcagctccgg cacatgcgcg gagagcgtta
     6301 ccagcaccgg caggaaggcg atcaacgtca ttatggcgtt gataaaactg acgcccatat
     6361 tctccagcgt tgaagcaaaa cgcatggtgt cttcctgcac acgctgtgcg gcaccttcga
     6421 tatgacgcag ttgttgccag ttcgccatgt aatattcgtt catcgccgta cgccagcgga
     6481 acacgtagtg actgacaaag aagttgttca gtacactgat caccactgcg atcagcgcaa
     6541 tccccagaaa gacgcccact tcgcggtaaa attgttcgat agtgacttta tgcggcgaac
     6601 ttagcgcggt ttgaatcaga tcatagaacg gcgcatacca ggcgttgacg gcgaccccga
     6661 cttccaccaa aaaccaagtg acgaagataa tcagcgcagt accgagtatt gaccagtatt
     6721 gccaacggtg cgggctgtag ataaaccaga aaaatgcaaa aagtcctacg caaacaatgt
     6781 agtaagcgta aaaaatcagg aaatccaacg accagaaacg cgcggcgcta atcgggatct
     6841 gcccggaagc gccggtgata tgtgccaccc agtcaccccc accggcttgc cagaagataa
     6901 cggcaatcaa tgcccaaaca aaggccgaga gaaaaaacgt tcccggcttt gggaaaaaag
     6961 acttaaacat atgcactcct gctaacttct tatcgtttta acaactgctg tgtattaacc
     7021 gcatgaccgc tacaaagcgt cagccaaatg aaaccgccca ttgtgaccca tagcaaggcg
     7081 cttagttcgc cagcctgacc ataagaattg tttcgacgga tttcacaaac caaagattgt
     7141 ttacataagc ggattaacac gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa
     7201 gatcgtgttt acgcgccgtt acgtttttaa cactattatt gcactaattc gccctttgca
     7261 atccatcaat gagtagtata aatacgctca gttaccttca ttcaatctat ggacaccacc
     7321 gttgaaacgt agtctgcttt tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca
     7381 tgcggcgcag ccgatcactg aaccggagtt tgcctctgat attgtcgatc gttatgccga
     7441 tcatattttt tacggcagcg gtgccacggg gatggcgctg gttgttatcg acggtaacca
     7501 gcgcgtcttt cgtagttatg gcgaaacgcg acctggtaat aacgttcgcc cgcagctgga
     7561 ttcggtcgtg cgtattgctt ccctcaccaa actaatgacc agtgaaatgc tggtgaaatt
     7621 gctcgaccag gggaccgtaa agttaaacga tccgctaagt aaatatgctc cgccaggcgc
     7681 acgcgtgccg acctacaatg ggacgcccat taccctagtg aatctggcaa cccataccag
     7741 cgccctgccc cgtgaacagc ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac
     7801 gcgcgagcaa cgctggaaat acctttctac ggcgaagctg aaagccgcgc caggcagtca
     7861 ggcggcgtac tctaaccttg cgttcgacct gctggcagat gcactggcga atgcgtccgg
     7921 caagccttat acccagttgt ttgaagagca gattacccat ccattgggga tgaaagacac
     7981 cacctacacc ccctcaccgg atcagtgccg ccgtttaatg gttgctgagc gcggtgccag
     8041 tccgtgcaat aacacgctgg cggcaattgg cagcggcggc gtctattcca cgcctggcga
     8101 tatgatgcgc tggatgcagc agtatttgtc atctgatttt tatcagcgca gtaaccaggc
     8161 agatcgcatg cagacgttga tttatcagcg cgcgcagttt acgaaagtga ttggcatgga
     8221 tgtccccggc aaagcggatg cgcttggttt aggctgggta tatatggccc cgaaagaggg
     8281 tcgtccgagg attattcaga agacaggcgg tggtggcggt ttcattacct atatggcgat
     8341 gatcccgcag aagaatattg gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt
     8401 taaaaatatg agcgatggca tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt
     8461 ggttatcccc gcatcctgat taatactctg aaggcaaacg gttgatagcg accagtttgc
     8521 ctttattcat ttcgatatag ccgcctttac gcaaagcagc gagaacttct gcgacgacag
     8581 aacgcgatat gcgtgtacgt tgatggatat aattcatcac accaatacgt gatcgcaatt
     8641 cttcattcca gtcaatcatt gataataatg ttgcgcgggt ttgtttggct ggtagatgat
     8701 atcccgtaca atttccttct gatattaatt tgtatggtat atcgtttttc attaaaccat
     8761 cagccagccc cataatataa ggagcctggg taataccgat aagtacgttt tcttctctac
     8821 gcagagagat aactccttct aaaataacga aagtatcttc gttattgaca tcactggaaa
     8881 atataacttg cttttcatta ttaaactcga agcgcgtacc gtatctggac aaacatttat
     8941 cgagcttacc aaattcctga agaggtttaa ctacagataa catttgcgcg tcctttgcag
     9001 taatgcccgt caaatccttg acgggcatta tttagattaa attaccagta tttcttcgga
     9061 gtgaagaata ttaccaagta tatttaacac ccacgttcgc ggaccagtct tgatctacgt
     9121 caccaccacc gaggtagtta gcatcggtat aggcgctgaa gttcttggtg aagctgaact
     9181 gagtacccag accaacacgt accgcagacc cttcagtacc gttatcgatg gaatcaccgt
     9241 tcacatcgtt atcgttgtta gagtcgtcgt agacgtaagc cagtttgaag tacggagtca
     9301 gagcctgatc ttcgctgtag gtgaaggtat aacctgcatc tacacccagt tcataacgca
     9361 tgctgtcgta aggctgaccg tcaactttca tgtcgttgct cagctggtag tcatcaccag
     9421 actggaacag accagaaatg ctgccgtaag gagtcacgta accagcatca cccagtttga
     9481 agtcgtaacc ggttttcaaa ccgaagcccc aagcgtcgga gttggtgcta ccgtcaacgt
     9541 aagtaccgtt gctcatggtt gcagacaggt cgttgttgaa gtgagagtag ctcaagc
//
LOCUS       DAJVRP010000128         9484 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVRP010000128 DAJVRP010000000
VERSION     DAJVRP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.128"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..180)
                     /locus_tag="OQN12_003538"
     CDS             complement(<1..180)
                     /locus_tag="OQN12_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655734.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLG"
     gene            700..1395
                     /gene="minC"
                     /locus_tag="OQN12_003539"
     CDS             700..1395
                     /gene="minC"
                     /locus_tag="OQN12_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1655735.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1419..2231
                     /gene="minD"
                     /locus_tag="OQN12_003540"
     CDS             1419..2231
                     /gene="minD"
                     /locus_tag="OQN12_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1655736.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2235..2501
                     /gene="minE"
                     /locus_tag="OQN12_003541"
     CDS             2235..2501
                     /gene="minE"
                     /locus_tag="OQN12_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1655737.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2873..3217)
                     /locus_tag="OQN12_003542"
                     /pseudo
     CDS             complement(2873..3217)
                     /locus_tag="OQN12_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3252..3371)
                     /locus_tag="OQN12_003543"
     CDS             complement(3252..3371)
                     /locus_tag="OQN12_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1655738.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3332..3517)
                     /locus_tag="OQN12_003544"
     CDS             complement(3332..3517)
                     /locus_tag="OQN12_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655739.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3618..3791
                     /locus_tag="OQN12_003545"
     CDS             3618..3791
                     /locus_tag="OQN12_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655740.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3793..4137
                     /locus_tag="OQN12_003546"
     CDS             3793..4137
                     /locus_tag="OQN12_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS1655741.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4141..4476
                     /locus_tag="OQN12_003547"
     CDS             4141..4476
                     /locus_tag="OQN12_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1655742.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4533..6854)
                     /locus_tag="OQN12_003548"
     CDS             complement(4533..6854)
                     /locus_tag="OQN12_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1655743.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6971..7180)
                     /locus_tag="OQN12_003549"
     CDS             complement(6971..7180)
                     /locus_tag="OQN12_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655744.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7931..9454)
                     /locus_tag="OQN12_003550"
     CDS             complement(7931..9454)
                     /locus_tag="OQN12_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1655745.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2660 a         2200 c         2078 g         2546 t
ORIGIN      
        1 gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac aaatatacgg
       61 gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc cagacgccat
      121 tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca tcatattcat
      181 agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc aatccgtaat
      241 caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac gtcttctgcg
      301 gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct gttctttaat
      361 gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata cacttgcctc
      421 aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg gaaaaaggaa
      481 gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag ggtatttttt
      541 aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata aatgggaggg
      601 tgacttgcct caatataatc cagactataa catgccttat agtcttcgga acatcatcgc
      661 gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac tccaatcgag
      721 cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga acctaaggtt
      781 atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa acatgccccc
      841 gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat gcataaggcg
      901 gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc gcaacttaaa
      961 gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa agcgccacgt
     1021 ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa aacgcgttta
     1081 atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga tctgattgtt
     1141 acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca tgtctatggc
     1201 atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca aatattttgt
     1261 acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag tgatcaaatc
     1321 ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc tttgaccgtt
     1381 caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt attgttgtta
     1441 cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc actggtttgg
     1501 cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt aatctcgacc
     1561 tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt cagggcgatg
     1621 caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat attcttccgg
     1681 catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa gttcttgatg
     1741 atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg attgaaaccg
     1801 gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac ccggaagtct
     1861 cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca cgccgcgcag
     1921 aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac ccaggccgcg
     1981 taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc atcaaactcg
     2041 tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt gaaccggtca
     2101 ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa cgtctgttgg
     2161 gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc aaacgcttgt
     2221 tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag aacacagcca
     2281 acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc gatgcagaac
     2341 cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa tacgtacaaa
     2401 ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt tctattcttg
     2461 agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt aaaaacgcat
     2521 ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat ccacgttgtg
     2581 gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc cccctttaca
     2641 ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt aaataaccat
     2701 gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt cattgttctc
     2761 ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga attttctctt
     2821 tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat ggtcagaacg
     2881 accagttcac accagccacc gcgttccacg gggactccac accggcacca tggctatacc
     2941 ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct gcctggtata
     3001 ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact ttcacccgat
     3061 tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt tccataccgt
     3121 tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata ctgcgggtat
     3181 cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt gacctaagct
     3241 ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag agacagcgca
     3301 ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata tcccgcataa
     3361 gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa atcactcttt
     3421 tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa aataaaatga
     3481 ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac aataatattg
     3541 ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca cttcttgaat
     3601 aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt cacgtaaaaa
     3661 acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat agcgagcgtt
     3721 ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact tacttaatac
     3781 cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc attgttctgc
     3841 tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct aggcgcaggc
     3901 gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc tgttggcgct
     3961 ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa aggtgcaaaa
     4021 gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct cgaaggggcg
     4081 attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat gaaataagga
     4141 atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc aatgacctgg
     4201 gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac ttgcgcagaa
     4261 gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc ggtaatgggt
     4321 aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact gagcgccaaa
     4381 gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt gatcacggca
     4441 attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac cgcgtcgatg
     4501 aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag gcccaccatc
     4561 actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc ccagacgctg
     4621 gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt actcacccca
     4681 tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt atggatccag
     4741 ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt ctcctgagta
     4801 cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc attctcaata
     4861 cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat agcctgtgcc
     4921 tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt attcagggca
     4981 aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga ttcataggct
     5041 aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga gtcaatgtat
     5101 gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc gctatagcct
     5161 ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt gtagtcgcgg
     5221 gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc accggttaac
     5281 agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg tccgttgctg
     5341 tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga acgctcctgt
     5401 tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg cataacaaaa
     5461 agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg caggacgggg
     5521 gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc gctcgttgcc
     5581 tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg ggttaatgaa
     5641 tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt tgattccccc
     5701 tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc tgcattggta
     5761 actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc cgtagctgaa
     5821 ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac gtaattttca
     5881 gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat atagaccgta
     5941 ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca ggtactatta
     6001 tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc tgaacccgtt
     6061 atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat aaaaacatct
     6121 gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt tttatggtag
     6181 aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt tgcttcctga
     6241 ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc agatgtattt
     6301 ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg acccgttgta
     6361 taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc atgagcattg
     6421 tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt ataatcaagc
     6481 gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc tgttccgtgg
     6541 gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc attacttcct
     6601 gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct tccgggagat
     6661 gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt atcatctccg
     6721 tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt gtcagtattc
     6781 aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt gacaatcgtg
     6841 tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct agctgttaat
     6901 gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac aatgacgcca
     6961 ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt ccattacccg
     7021 ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt gccacagcat
     7081 gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc gccagagcaa
     7141 taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta tccattcatt
     7201 ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa acggagggta
     7261 aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata tataaatgat
     7321 aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac tttggggtga
     7381 ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc catggtaatt
     7441 atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta tttgcgcaat
     7501 aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag ccgcatactg
     7561 acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt tctcttaaat
     7621 cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat cgtcacaagc
     7681 acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt gatcagcata
     7741 aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact attattcatg
     7801 ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa taatgaacaa
     7861 tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact atggccttac
     7921 tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata attttgacca
     7981 ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg atattatttt
     8041 ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata ctgatcgaaa
     8101 gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg tctgcgttta
     8161 cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca tgaagataag
     8221 aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca
     8281 gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc tcggtgactt
     8341 ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga
     8401 caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga aaaccttccg
     8461 gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg ctttgtgtaa
     8521 gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt gaaatatatc
     8581 caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac
     8641 catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc cgtcgcagga
     8701 tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca gcggcgcagg
     8761 cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg tagaagctaa
     8821 agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga accttctcgg
     8881 tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata atgtcatccg
     8941 ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg cgaatatgtt
     9001 gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag agcaggatag
     9061 gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc gggacaacag
     9121 gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact attccctgtt
     9181 tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca agctgatatt
     9241 gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc gcctgtgacg
     9301 cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc aggtactctg
     9361 cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata aacaagcata
     9421 tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca aagggtttaa
     9481 taca
//
LOCUS       DAJVRP010000129         9469 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVRP010000129 DAJVRP010000000
VERSION     DAJVRP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.129"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OQN12_003551"
     CDS             complement(383..1360)
                     /gene="epmA"
                     /locus_tag="OQN12_003551"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1655746.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1685..3493
                     /gene="frdA"
                     /locus_tag="OQN12_003552"
     CDS             1685..3493
                     /gene="frdA"
                     /locus_tag="OQN12_003552"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1655747.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3486..4220
                     /gene="frdB"
                     /locus_tag="OQN12_003553"
     CDS             3486..4220
                     /gene="frdB"
                     /locus_tag="OQN12_003553"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1655748.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4231..4626
                     /gene="frdC"
                     /locus_tag="OQN12_003554"
     CDS             4231..4626
                     /gene="frdC"
                     /locus_tag="OQN12_003554"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1655749.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4637..4996
                     /gene="frdD"
                     /locus_tag="OQN12_003555"
     CDS             4637..4996
                     /gene="frdD"
                     /locus_tag="OQN12_003555"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1655750.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5059..6192
                     /locus_tag="OQN12_003556"
     CDS             5059..6192
                     /locus_tag="OQN12_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1655751.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6281..6814
                     /gene="blc"
                     /locus_tag="OQN12_003557"
     CDS             6281..6814
                     /gene="blc"
                     /locus_tag="OQN12_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1655752.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OQN12_003558"
     CDS             complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="OQN12_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1655753.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="OQN12_003559"
     CDS             complement(7304..7450)
                     /gene="ecnB"
                     /locus_tag="OQN12_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1655754.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="OQN12_003560"
     CDS             complement(7561..7686)
                     /gene="ecnA"
                     /locus_tag="OQN12_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1655755.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7738..8304)
                     /gene="efp"
                     /locus_tag="OQN12_003561"
     CDS             complement(7738..8304)
                     /gene="efp"
                     /locus_tag="OQN12_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1655756.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8346..9374
                     /gene="epmB"
                     /locus_tag="OQN12_003562"
     CDS             8346..9374
                     /gene="epmB"
                     /locus_tag="OQN12_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1655757.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2398 a         2435 c         2431 g         2205 t
ORIGIN      
        1 aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa
       61 cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc
      121 aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt
      181 tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt
      241 aaaaaccaat acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc
      301 tttaacggaa aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg
      361 ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca
      421 gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac
      481 agtcaggcat accaactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca
      541 aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat
      601 ccgttaattc atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt
      661 cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt
      721 ggtacacaaa ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca
      781 attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg
      841 ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca
      901 agaaagcttg ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga
      961 gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca
     1021 tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct
     1081 ggaataccgg cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca
     1141 ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga
     1201 ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc
     1261 cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag
     1321 gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg
     1381 acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa
     1441 cagcggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat
     1501 cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac
     1561 ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc
     1621 gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa
     1681 tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg
     1741 tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt
     1801 atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga
     1861 tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga
     1921 gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact
     1981 gtggggatgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg
     2041 catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac
     2101 gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct
     2161 ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg
     2221 cacgttggtg cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt
     2281 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
     2341 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg
     2401 ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa
     2461 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
     2521 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
     2581 gcgtaaaggc aacaccatcc ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca
     2641 cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
     2701 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat
     2761 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
     2821 tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga
     2881 actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
     2941 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
     3001 tctagttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc
     3061 aatggaagaa ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
     3121 gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt
     3181 caacaccgac ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat
     3241 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg
     3301 ttgcaccgag cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga
     3361 tggcacgact cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg
     3421 cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa
     3481 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
     3541 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
     3601 atgcgctggg ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc
     3661 gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
     3721 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
     3781 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
     3841 cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc
     3901 agatggcaaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
     3961 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
     4021 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
     4081 agaacggtgt atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg
     4141 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
     4201 cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
     4261 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
     4321 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg
     4381 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
     4441 atcattaacc tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg
     4501 gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
     4561 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
     4621 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
     4681 gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct
     4741 ggtgggtatt ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt
     4801 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
     4861 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc
     4921 tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg
     4981 tgtcgttaca atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt
     5041 gtatggaaac cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct
     5101 tactccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg
     5161 cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct
     5221 tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg
     5281 ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt
     5341 gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct
     5401 aaacagtgga atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca
     5461 ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg
     5521 cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc
     5581 ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc
     5641 ttccagccac tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat
     5701 tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct
     5761 gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta
     5821 cccccccttg atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc
     5881 tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc
     5941 aatcctgaca gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta
     6001 aaagcgatta agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg
     6061 accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg
     6121 ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac
     6181 gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc
     6241 tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
     6301 tgccgcagcg acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg
     6361 cgtgaccgta gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc
     6421 ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg
     6481 tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca
     6541 gcagagtgaa gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc
     6601 attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca
     6661 tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat
     6721 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
     6781 taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat
     6841 ccccaatacg attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag
     6901 cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt
     6961 ccctaccggt aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt
     7021 cacggtaata acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc
     7081 ccataccact tccagcagac cagcaataac taagataatc caggacatat caggctccgg
     7141 aaatttgggg ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg
     7201 ttgaacggat attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc
     7261 agaaaaaaca aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg
     7321 ttgctgcgcc agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg
     7381 tgttgcaggc agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct
     7441 tcttcaccat aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa
     7501 taatagacaa catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa
     7561 ttagctggca gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca
     7621 agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt
     7681 catcatctct gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta
     7741 cttcacgcga gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg
     7801 tacgaacagc ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt
     7861 acctgcggta tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt
     7921 cggcggagta acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc
     7981 cagcagccat ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt
     8041 ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc
     8101 gacaacatca gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc
     8161 ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa
     8221 ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa
     8281 atcgttgcta tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc
     8341 acaaaatggc gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac
     8401 ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg
     8461 aaaaactgtt ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat
     8521 ttatcgatcg catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct
     8581 cgcaagatga gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca
     8641 gcgtagtgcc tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg
     8701 gctgcgcggt aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg
     8761 gcaacaagcg taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg
     8821 agatgatttt ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc
     8881 tcacacaact ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga
     8941 ttgtgatccc ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc
     9001 aaatcttgct ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg
     9061 cgatggctaa attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg
     9121 gtgtgaacga taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg
     9181 tgatgccgta ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga
     9241 gtgatgacga agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg
     9301 tgccgaaact ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc
     9361 tacgccagca gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac
     9421 gcttatcagg cctacatcgt gcattgaaat tgaatggatt tgcaatgaa
//
LOCUS       DAJVRP010000130         9255 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVRP010000130 DAJVRP010000000
VERSION     DAJVRP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.130"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OQN12_003563"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OQN12_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1655758.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OQN12_003564"
     CDS             1828..3147
                     /locus_tag="OQN12_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1655759.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OQN12_003565"
     CDS             3445..3486
                     /locus_tag="OQN12_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655760.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OQN12_003566"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OQN12_003566"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCS1655761.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OQN12_003567"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OQN12_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OQN12_003568"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OQN12_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OQN12_003569"
     CDS             5815..6711
                     /locus_tag="OQN12_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1655762.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7694
                     /locus_tag="OQN12_003570"
     CDS             6708..7694
                     /locus_tag="OQN12_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1655763.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7687..8613
                     /locus_tag="OQN12_003571"
                     /pseudo
     CDS             7687..8613
                     /locus_tag="OQN12_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OQN12_003572"
     CDS             complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="OQN12_003572"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1655764.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2264 a         2260 c         2319 g         2412 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 aaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctgccgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctaggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gctggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
     7621 cgctgacagc gtgtggtgac aacaaccagc actgcgcctg ttggtatccg cagcaggagg
     7681 tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt tccggcccgt
     7741 aaaaactggc ttggtaaatc tacggaacat gttcatgcca ttaatggtat tgatttacag
     7801 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
     7861 gcacagcttt taatgggcat gctgcaaccg agccacgggc agtacatccg ttcaggctca
     7921 caacgcatta tgcagatggt gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg
     7981 gtgtggcgca tcatcacaga atcgctctgg atagctaagc gtagtagtga acaacagcgg
     8041 cgagcgttgg cagaggagtt ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
     8101 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
     8161 tcgcagcctg acgtgattgt gcttgatgag ccaacctcgg cgctggatat ctccgtgcag
     8221 gcgcagatcc tcaatttgct ggtaacgcta caggaaaatc acgggctgac ttatgtgctg
     8281 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
     8341 gggcagattg tagaactggg cgacgcgcaa caggtgttga ctgcttcggc acatccatac
     8401 acccgattat tgctggattc cctccccgcc attgataaac cgctggagga agaatgagca
     8461 ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt tttctacgaa
     8521 cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattagcaat aagggaggac
     8581 ggacgtgagc tccggtgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
     8641 cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
     8701 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
     8761 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
     8821 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatt gatgttggcg
     8881 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
     8941 cgccaatcag ttcttcaggg ttggttcctt tttcgccttc aaaacgcgtg ttaaatccat
     9001 acggctgttg gttcagcacg ccgctctcgg tggatactgt tcccttcccg cgtttgatat
     9061 cgccttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc ctgtgggctt
     9121 aaaaaggaaa ttatagacta cgtgagaaac agcacgagga taagcaggat attccgaata
     9181 aaatacggcg aaaatattac ttctgcactt aaattaaatt cccacaatag ataaccggga
     9241 atatatatct caaga
//
LOCUS       DAJVRP010000131         9153 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVRP010000131 DAJVRP010000000
VERSION     DAJVRP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.131"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..923
                     /locus_tag="OQN12_003573"
     CDS             <1..923
                     /locus_tag="OQN12_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1655765.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="OQN12_003574"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="OQN12_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1655766.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OQN12_003575"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OQN12_003575"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1655767.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="OQN12_003576"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="OQN12_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1655768.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="OQN12_003577"
     CDS             4446..4805
                     /locus_tag="OQN12_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1655769.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="OQN12_003578"
     CDS             complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="OQN12_003578"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1655770.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6047..6237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6302..8146
                     /gene="btuB"
                     /locus_tag="OQN12_003579"
     CDS             6302..8146
                     /gene="btuB"
                     /locus_tag="OQN12_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCS1655771.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8091..8948
                     /gene="murI"
                     /locus_tag="OQN12_003580"
     CDS             8091..8948
                     /gene="murI"
                     /locus_tag="OQN12_003580"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1655772.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2217 c         2469 g         2373 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg agaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5581 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5641 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5701 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5761 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5821 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5881 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5941 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     6001 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     6061 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     6121 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6181 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6241 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6301 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6361 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6421 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6481 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6541 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6601 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6661 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6721 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6781 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6841 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6901 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6961 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     7021 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     7081 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     7141 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7201 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7261 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7321 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7381 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7441 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7501 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa atctggggca
     7561 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7621 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7681 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7741 gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7801 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7861 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7921 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7981 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     8041 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     8101 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     8161 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8221 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8281 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8341 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8401 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8461 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8521 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8581 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8641 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8701 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8761 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8821 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8881 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8941 taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag
     9001 gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgac
     9061 gggtcagcgg ggttctcctg agaattccga cagagaaagc aaaaataaat gcttgactct
     9121 gtagcgggaa agcgtattat gcacaccccg cgc
//
LOCUS       DAJVRP010000132         9015 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVRP010000132 DAJVRP010000000
VERSION     DAJVRP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.132"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(98..1474)
                     /gene="trkA"
                     /locus_tag="OQN12_003581"
     CDS             complement(98..1474)
                     /gene="trkA"
                     /locus_tag="OQN12_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1655773.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1496..2785)
                     /gene="rsmB"
                     /locus_tag="OQN12_003582"
     CDS             complement(1496..2785)
                     /gene="rsmB"
                     /locus_tag="OQN12_003582"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1655774.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2831..3778)
                     /gene="fmt"
                     /locus_tag="OQN12_003583"
     CDS             complement(2831..3778)
                     /gene="fmt"
                     /locus_tag="OQN12_003583"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1655775.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3793..4302)
                     /gene="def"
                     /locus_tag="OQN12_003584"
     CDS             complement(3793..4302)
                     /gene="def"
                     /locus_tag="OQN12_003584"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1655776.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4450..5574
                     /gene="dprA"
                     /locus_tag="OQN12_003585"
     CDS             4450..5574
                     /gene="dprA"
                     /locus_tag="OQN12_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1655777.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5546..6019
                     /locus_tag="OQN12_003586"
     CDS             5546..6019
                     /locus_tag="OQN12_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1655778.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6048..6590
                     /locus_tag="OQN12_003587"
     CDS             6048..6590
                     /locus_tag="OQN12_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCS1655779.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6595..7167
                     /gene="tsaC"
                     /locus_tag="OQN12_003588"
     CDS             6595..7167
                     /gene="tsaC"
                     /locus_tag="OQN12_003588"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1655780.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7172..7990
                     /gene="aroE"
                     /locus_tag="OQN12_003589"
     CDS             7172..7990
                     /gene="aroE"
                     /locus_tag="OQN12_003589"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1655781.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7987..8244
                     /locus_tag="OQN12_003590"
     CDS             7987..8244
                     /locus_tag="OQN12_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1655782.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8220..8774)
                     /locus_tag="OQN12_003591"
     CDS             complement(8220..8774)
                     /locus_tag="OQN12_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1655783.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2257 a         2188 c         2247 g         2323 t
ORIGIN      
        1 tctaacaaat gttaagccat tttcctgcca ggaatgaatt taattaatcc ttgataatca
       61 aatggaaata ataaggcgtc attagacgcc ttattaatta caagaagaaa gggcttggct
      121 ggaagagtct ttcgacgtcg gtaataaact ttttatctgt gaggaacata attacgtgat
      181 cgccttgctc aatgcgcaga ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg
      241 ctccaataat cgttcctggc ggtagcttga tttcgtcaat gactctgccg acaacgcgtg
      301 aggtgctttc atcaccgtga gcaacggctt caatagcttc tgctacgccg cggcgcaatg
      361 aggaaacacc aacaatatct gcttttcgca catggctaag caacgcagaa atagttgctt
      421 gttgtggtga aattgcaata tcgatcacgc tcccctgaac cagatccaca taagcgcgac
      481 gctggatcaa taccatcacc tttttcgcac ccatacgttt ggcaagcatg gcggacatga
      541 tattggcctc gtcatcgttg gtgacggcaa taaacagatc aacttgatcg atatgttctt
      601 cggccagcag ttcttgatcc gacgcatcac caaaaaagac gatcgtattc tgcaactttt
      661 ccgccagttc ggcagcgcgc tgctgattac gttcgatgag tttaacgctg taatcttttt
      721 ccagacgacg cgccagccct gcaccgatat taccgccacc aaccagcatg atccgtttat
      781 acggtttttc cagtcgctgt aattcactca tcaccgcgcg gatatgctgt gaagcggcaa
      841 taaagaacac ttcatcacca gcttcaacaa tggtcgaacc ttgtggacga atggggcgat
      901 cgtggcggaa aattgctgcc acacgagtat cgatatgtgg catatgttcg cgcatggtcg
      961 aaagtgcatt accaatcagc gggccgccat aataggcttt aactacagcg aggctgactt
     1021 taccctcagc gaagttcacc acctgcaatg cgcctggata ctcaatcagt cggtaaatat
     1081 tatcgataac caactgctct ggtgcgatca gatgatcaat cggtacagca tctgaatgaa
     1141 atagcttatc ggcatcgcgt acgtagtctg gtgagcggat gcgagcgatg cgattagggg
     1201 tgttgaaaag tgagtaggct acctggcagg caaccatatt ggtttcatct gaactggtta
     1261 cagcaaccag catatcggcg tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc
     1321 catgcccctg cacgactcgc aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt
     1381 tggtatcgac aacagtaata tcgttgttct cgccaaccag gttttccgcc agtgtgccgc
     1441 caacctggcc ggcacccaga atgataattt tcatcagtcg cgacccgtta tctcatcact
     1501 ttttgattag cttagcgtaa aagaagccgt cgccctcttc ggcaccaggc tgattttgtt
     1561 tacccggttg ctctggtgtt cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa
     1621 aggctttaat ctgcaggcta ttctcttccg gtaacaccga acaggtggca tagaccaggg
     1681 ttccaccgga ttttaaatgc ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga
     1741 gttccgggat atcgcgatcg cgacgtaacc atttaatatc tggatggcga cgaatcaccc
     1801 ccgttgctga acaaggcgca tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt
     1861 gggaagggta acggccatca ccttgtttca cggttgcctt cataccaagg cgttttaaat
     1921 tgtcgtaaat gcgagagagg cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg
     1981 gtgccacctc aaggatatgc gttgttttac cgccgggggc ggcacaaaga tccaagatgt
     2041 gttcaccgtt ttgtggcgca agccaggtca tgcaaccttg tgctgatgca tcctgaacgg
     2101 taacccatcc gtcttcaaaa ccaggtagcg catgaacagg tgcaggtgtt tccagccgta
     2161 cagcatcagg gtaatccgca tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa
     2221 gccagctgtc gcgggaatga tgcgtacgat taatacgcag ccacattggc ggacgctggt
     2281 tattggcttc ggcgatggat tgccactgct ctggatacgc tttttgcaga cgcttcagca
     2341 accaggaagg atgcagataa cgtgcatcac gggtattaaa ctcggctaat aactcttctt
     2401 gccgacgctg gaactggcgt aatacaccgt taatcaaccc tttaagttgc ggacgcttaa
     2461 ttgcgatagc gccttcaacc gtttcagcca gcgcagcatg aggtggaatg cgggtataaa
     2521 gcagttgata caaaccaacc ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg
     2581 gacgggccat taacttatta atcagccagt ctaactgcga aagcgtacgc agtacgccaa
     2641 agcacaactc ttgaagaagt gctttgtctt tatcggaaac tttttgctgg agcggtggca
     2701 gaatgttgct taatgattgc ccttgctcga cgacttgttc aacggcctgg gccgccatgc
     2761 tacgtaaatt acgttgtttt ttcataagta taaaaatgct cggcaagacc gggcttagaa
     2821 gagtggacta tcagaccaga cggttgcccg gaacaaacca ttcccgacga gagttcagga
     2881 ggtcttgcgc gctcatcgct ttcttacccg caggttgtaa cgagagcagg ttcaggatgc
     2941 catcaccagt cgcaacctga atgccttgtt tgttggcttc aaggatcgtt cctggtgcag
     3001 cgttggttgc cgtatcaatg accgacgctt tccagacttt aaccggctgt ccttcaattt
     3061 ccagccagct cattggccat gggttgaaag cgcgaatgca gcgttcaagc tgtgctgccg
     3121 aaagtgacca gtcaatacgc gcttcttctt tactcaactt ctcggcgtaa gtgacaagag
     3181 tttcgtcctg aacttctggt ttcgccgtgc cgtctgccaa ttgtttcaac gtggtgataa
     3241 gcccttgtgg gccaagctct gccagcttgt cgtacagcgt accactggta tcttctgcag
     3301 taatcgggca ggagagctta tagagcatgt caccggtgtc taaaccaaca tccatttgca
     3361 taatggttac accagtttct gcatcacccg cccatagtga gcgttggatt ggtgcagcac
     3421 cgcgccagcg tggcagcagt gaaccatgaa tgttgataca gccaagacgc ggcatctcca
     3481 gcacagcttt aggcagaatt aaaccatagg cgacgacgac cataacatca gcctgcaaat
     3541 cggcgaccag ttgctggttt tcttgtggac gcagggaaac aggttgaaaa acgggcagac
     3601 cttcttcctc agccagaact ttaatcgggc tgggcatcag ttttttaccg cgtcctgccg
     3661 gtcggtctgg ctgggtgaac acgccaacga cgttatgacc agaagacaac agcgcgtcga
     3721 gatgacgcgc tgcaaagtca ggtgtacccg caaaaataat acgtagtgat tctgacacgt
     3781 tagttcttat ccttaagccc gggctttcag acgatccagt ttttcaactt tctgacgaat
     3841 tcgttgttgt ttcagcggtg acagataatc cataaacagt ttgccaacca ggtgatccat
     3901 ctcatgctga atacagatag ctaacagacc ctctgcttcc agttcaaatg gtttaccgtc
     3961 gcggtcaagg gcgcgaattt taactttctc tgcgcgcggc actaaagcac gttgttcagg
     4021 gatcgacagg caaccttctt caatgcctgt ttcgccgctt ttttctaaaa gctccggatt
     4081 gattaacacc agccgttcgt cacggttttc cgaaacatca ataacaatga tacgttgatg
     4141 gatatcaacc tgggttgccg ccaggccaat accttcttct gcgtacatcg tctcgaacat
     4201 atcatcgacg atacgctgaa tttctgcatt cacttcttct accggtttag caactttgcg
     4261 aagccgctcg tccggaatat gtaacacttg caaaactgac ataaatctcc agagatgtgt
     4321 tcaggagtta gaaagattat ttcttctatt ctagacaaat ccccctctga ttgacagcat
     4381 cacttgattg acagcatcac tgaccaatcg caaagattgc taaggcttgc ttatggcagg
     4441 gagataagga tggtcgatac agaaatttgg ctgcgtttaa tgagcatcag cagcttgtac
     4501 ggcgatgata tggtccgtat cgctcactgg ctggcaaaac agtcgcatat tgatgcggtt
     4561 gtattgcagc aaacagggct tacattgcgg caggcacaac gctttctttc atttccacga
     4621 aagagtatcg aaagctcact ttgttggttg gagcaaccca accatcattt aatccctgcg
     4681 gacagcgaat tttatcctcc tcaacttcag gcgacgacag attaccccgg cgcactgttt
     4741 gttgaaggag aactgcacgc gctgcattca tttcagcttg ccgtagtggg gagtcgggcg
     4801 cattcatggt atggcgagcg atgggggcga ttattttgcg aaactctggc gacgtgtgga
     4861 gtgacaatta cgagtggact ggcgcgtgga atcgatggtg tggtgcataa agcggcctta
     4921 caggtaaatg gcgtcagcat tgctgtattg gggaatggac ttaataccat tcatccgcgt
     4981 cgccatgccc gactggctgc cagtctgttt gaacagggtg gcgctctcgt ctcggaattt
     5041 cccctcgatg ttccacccct tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt
     5101 ctaagtaaag gtgtactggt ggtggaagcg gctttgcgca gtggttcgct ggtgacagca
     5161 cgttgtgcgc ttgagcaggg gcgtgaagtt tttgccttgc caggtccaat agggaatccg
     5221 ggaagcgaag ggcctcactg gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa
     5281 gaaattctgg aaaacttgca atttggattg cactggttgc cagacgcccc tgaaaattca
     5341 ttttattcac cagatcagca agacgtggca ttgccatttc ctgagctcct ggctaacgta
     5401 ggagatgagg taacacctgt tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag
     5461 gtagttactc aactactcga actggagtta gcaggatgga tcgcagctgt acccggcggc
     5521 tatgtccgat tgaggagggc atgccatgtt cgacgtacta atgtatttgt ttgaaaccta
     5581 tattcacact gaagctgagt tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga
     5641 cgcaggtttt gagcgagaag atatctacaa tgccctgcta tggctggaaa aacttgctga
     5701 ttatcaggaa gggttggcag aaccgatgca actggcctcg gatcctctct ccatgcgtat
     5761 ttatacaccg gaagagtgtg aacgactgga tgccagctgc cgtgggtttc tgcttttcct
     5821 tgagcagatt caggtgctca accttgaaac tcgtgaaatg gtgatagagc gagtgctcgc
     5881 gctggataac gcagagttcg aactggatga tctgaaatgg gtgatcctga tggtgttgtt
     5941 caacatcccg ggctgcgaaa atgcgtacca gcaaatggaa gaattactct ttgaagtgaa
     6001 tgaaggtatg ctgcattaat catttcttta attcagcata agttgttatg gcaaaatcag
     6061 cactgttcac ggtgcgtaat aatgagtcct gcccaaagtg cggggctgaa ctggttattc
     6121 gatccgggaa acacggtccg tttcttggat gctcacagta tccggcgtgt gactacgtcc
     6181 gtcctctgaa atcttcagcg gatggacata ttgtcaaagt tctggagggg caggtttgcc
     6241 ccgtatgtgg cgcaaatctg gtattacgcc agggacgctt tggtatgttt attggttgca
     6301 gtaactaccc tgaatgcgaa cataccgaac ttatcgataa accggacgaa acagcaatta
     6361 catgccccca atgtcggacg ggccatctgg tccagcgccg ctcccgttat ggcaaaacat
     6421 ttcactcctg tgatcgctac ccggagtgtc aatttgccat taacttcaaa ccaatagctg
     6481 gagaatgccc tgagtgtcat tatccgctac tcatcgaaaa gaaaaccgcg cagggtgtaa
     6541 aacacttttg tgccagtaaa caatgtggaa agccggtttc ggcggaataa taacgtgaat
     6601 aataacctgc aaggagacgc tatcgcagct gcgatagatg tcctcaatga aaaacgtgtc
     6661 atcgcctatc caacggaagc cgttttcggt gtcgggtgcg atcctgatag cgaaacagca
     6721 gtgatgagac tgttggagtt aaaacagcgt ccggttgata aggggctgat tttaatcgcg
     6781 gcaaattacg agcagcttaa accctatatt gatgacacca tgttgactga cgtgcagcgt
     6841 gaaaccattt tttcctgctg gccaggtcct gtcacctttg tctttcccgc gcctgcgaca
     6901 acaccgcgct ggttgacggg ccgctttgat tcgcttgctg tacgagtcac cgaccatccg
     6961 ttggtggttg ctttgtgcca ggcttatgat aaaccgctgg tttctaccag tgccaacttg
     7021 agtggattgc caccttgtcg aacagtagac gaagttcgcg cacaatttgg cgcggcgttc
     7081 ccggttgtgc ctggtgaaac gggggggcgt ttaaatcctt cagaaatccg cgatgccctg
     7141 acgggtgaac tgtttcgaca ggggtaacat aatggaaacc tatgctgttt ttggtaatcc
     7201 gatagcccac agcaaatcgc cattcattca tcagcaattt gctcagcaac tgaatattga
     7261 acatccctat gggcgcgtgt tggcacccat caatgatttc atcaacacac tgaacgtttt
     7321 ctttcgtgct ggtggtaaag gtgcgaatgt gacggtgcct tttaaagaag aggcttttgc
     7381 cagagcggat gagcttactg aacgggcagc gttggctggt gcagttaata ccctcaagcg
     7441 gttagaagat ggacgcctgc tgggtgacaa taccgatggt gtaggcttgt taagcgatct
     7501 ggaacgtctg tcttttatcc gccctggttt acgtattctg cttatcggcg ctggtggagc
     7561 atctcgcggc gtactactgc cacttctttc cctggactgt gcggtgacaa tcactaatcg
     7621 gacggtatcc cgcgcggaag agttggctaa attgtttgcg cacactggca gtattcaggc
     7681 gttgggtatg gacgaactgg aaggtcatga gtttgatctc attattaatg caacatccag
     7741 tggcatcagt ggtgatattc cggcgatccc gtcatcgctc attcatccag gcatttattg
     7801 ctatgacatg ttctatcaga aaggaaaaac tccttttctg gcatggtgtg agcagcgagg
     7861 ctcaaagcgt aatgctgatg gtttaggaat gctggtggca caggcggctc atgcctttct
     7921 tctctggcac ggtgttctgc ctgacgtaga accagttata aagcaattgc aggaggaatt
     7981 gtccgcgtga atcaggccat ccagtttccg gacagggaag agtgggacga gaataaaaaa
     8041 tgtgtatgtt ttcccgctct cgtgaatggt atgcaactga catgcgcgat ctctggcgag
     8101 agtctggcgt atcgctttac tggagatacg ccagaacagt ggttagcgag ttttcgtcag
     8161 catcgctggg acctggaaga agaagcggaa aacttaattc aggaacaaag tgaagatgat
     8221 caaggctggg tctggttacc ctgatccaga tattcgtcct tccatttcac gtaattattc
     8281 gcggaatagc gtaacccagc cttctcttca tcacttaacg ggcggatctg tttgacgggg
     8341 ctaccgagat acagatatcc gctctccagc cgtttatttt gtgggaccag actacccgca
     8401 ccaatcatca catcatcttc tactattgcg ccatcaagta aaattgagcc catcccaacc
     8461 aaaactcgat tgccaatggt gcagccgtgg agcatcacct tgtgaccaac agtgacatct
     8521 tcgccaatgg ttaatgggtt gccatctggg ttgtacgagg atttatgagt gacatgcaac
     8581 atactgccat cctggatatt ggtgcgtgct ccgatctgta cataatgtac atctccacga
     8641 atcacaacga gcggccagat ccccacatca tcagccagac gaacgtcacc aatcacgaca
     8701 ctgctatcgt cgatcattac gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt
     8761 aaaacatcag acatacttac ctcagcaata aatgatttac taatgacttt gggggcatta
     8821 ttggccttgt gcaagtcttt tagtatgcaa aaaagcaccg ttttgtgtgc gattgcagca
     8881 aaaagggtga aaaaacaaca aacagaaaaa aagatcaaaa aaatacttgt gcaaaatatt
     8941 gggatcccta taatgcgcct ccgttgagac gacaacgtga aacacttcac aggatggtcg
     9001 gaacaacgaa gagaa
//
LOCUS       DAJVRP010000133         8989 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVRP010000133 DAJVRP010000000
VERSION     DAJVRP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.133"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            146..2330
                     /gene="espL4"
                     /locus_tag="OQN12_003592"
                     /pseudo
     CDS             146..2330
                     /gene="espL4"
                     /locus_tag="OQN12_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="OQN12_003593"
                     /pseudo
     CDS             complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="OQN12_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="OQN12_003594"
                     /pseudo
     CDS             complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="OQN12_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="OQN12_003595"
                     /pseudo
     CDS             complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="OQN12_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7423..8352)
                     /gene="metA"
                     /locus_tag="OQN12_003596"
     CDS             complement(7423..8352)
                     /gene="metA"
                     /locus_tag="OQN12_003596"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1655784.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8509..8952
                     /locus_tag="OQN12_003597"
     CDS             8509..8952
                     /locus_tag="OQN12_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCS1655785.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2357 a         2168 c         2166 g         2298 t
ORIGIN      
        1 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
       61 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
      121 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
      181 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
      241 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
      301 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
      361 ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata aaaacgaaaa
      421 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
      481 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
      541 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
      601 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
      661 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      721 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      781 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      841 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      901 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      961 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
     1021 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
     1081 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
     1141 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
     1201 aatcaccaac tcacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
     1261 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
     1321 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
     1381 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
     1441 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
     1501 ttacctaaac tggccacaac acatcattta gataatgaac aggtgtataa attcctgagt
     1561 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1621 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1681 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1741 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
     1801 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1861 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1921 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
     1981 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
     2041 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
     2101 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
     2161 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
     2221 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
     2281 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
     2341 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
     2401 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
     2461 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
     2521 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
     2581 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
     2641 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
     2701 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
     2761 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
     2821 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
     2881 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     2941 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     3001 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     3061 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     3121 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     3181 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     3241 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     3301 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     3361 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     3421 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     3481 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     3541 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     3601 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     3661 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3721 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     3781 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     3841 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     3901 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     3961 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     4021 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     4081 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     4141 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
     4201 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
     4261 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
     4321 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
     4381 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
     4441 tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg tagcccatat
     4501 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
     4561 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
     4621 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
     4681 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
     4741 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
     4801 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
     4861 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
     4921 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
     4981 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
     5041 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
     5101 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
     5161 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
     5221 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
     5281 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg tagctttttt
     5341 cgactcaccg tgcagcagac gccacatttt cgctgcgccc agttgcgcca gcgtgcattc
     5401 aggattgact gaaccgcgta atttcaccac atcttccgca ctgtatgggc gagtaatgcc
     5461 ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg tacgggtttt
     5521 catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta gcctggcagg
     5581 gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg
     5641 tcaaaacggc cctgggagaa acgttcttcg cccagttcgc tggcaatgac tttcatctct
     5701 tcgcccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct caacgttttt
     5761 tgatgatgga tccactgcca gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc
     5821 atcaggccat aaatcggcac gcagccgttg ccggagatcc acgcttcgat gtactgcaca
     5881 gccacgcgaa tgttagcgcg cataccttct tcggtgcgtt caccatcaca aggtgccagc
     5941 agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg
     6001 gagccgagaa tgtcgttgaa taccgccatt gccgtgtccg caaggcctgg gtgagcgatc
     6061 catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac tttgttgagc
     6121 acctggttat tacgctcttc atctttgctc ggaataaacg cggccatgcc gcccatcgca
     6181 aaagcaccgc gtttatggca ggttttaatc agcaggcgtg agtaagcatt caggaatggt
     6241 ttatccatcg tcacggcctg tctgtctggc aggacacgat cgggatagtt tttcaacgtt
     6301 ttgatatagc tgaagatgta atcccagcga ccgcagttca gaccaacaat atggtcgcgc
     6361 agcgcgtgaa ggatttcgtc catctggaac acggcgggca gcgtttcaat cagcaacgtc
     6421 gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa gacttcgctc
     6481 caccaggccg cttcctgcta ggactgggtt ttcggcagat agaaataggg accgctgccc
     6541 tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa caggctaccg
     6601 gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc gcgtacccga
     6661 caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc attggtatag
     6721 ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag
     6781 tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc gttcagtgcg
     6841 ttgatcacca tcttgcgctc taccgggcca gttatctcta cgcggcggtc ttctaagtcc
     6901 gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg aagccatttc cgaaataaaa
     6961 tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta
     7021 ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc ttcggcagta
     7081 agaatttgct tctcctgctc gccatacggc cttgtgaaag ccagttcatc ggttgttgtt
     7141 gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc tctgtgaacg
     7201 aaggaacgtt gtgcgctttt cgttcagcac aactaagact actcatttaa aatgcaaaat
     7261 caaaaacaat ttccatttta attaaaaata cttgataact tattgataac aatatgaata
     7321 aaatttattc acttgctgag gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga
     7381 ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca gcgttggatt
     7441 catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc agttggtaaa
     7501 cagcaaatta ccgtgactac gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg
     7561 gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgta aatattcctg
     7621 cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc gcttatcttt
     7681 gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca gatcggtgta
     7741 atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc
     7801 atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt aaacgccaga
     7861 gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg cggcctgtac
     7921 cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca gcacctgttt
     7981 gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg caccggttac
     8041 aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga agttgttcag
     8101 atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct gaatatcgac
     8161 ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg
     8221 catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg cacgagaagt
     8281 tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct cgtccggcac
     8341 acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga catccagata
     8401 gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt
     8461 caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat ggttattagt
     8521 attcgccgct cacggcatga ggaaggggag gaactcgttg cgatttggtg tcgatctgtc
     8581 gatgccaccc acgatttttt atcagcagag tatcgggccg agctggaaga gctggtgcgt
     8641 tcctttctgc cggaagcacc gttgtgggtc gcggttaatg aacgggagca gccggttggg
     8701 tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga tgtgtgcggc
     8761 tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga actgacaacc
     8821 aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg ttttaaggtt
     8881 acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat
     8941 gtgggggaat aagccgcccc tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAJVRP010000134         8897 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVRP010000134 DAJVRP010000000
VERSION     DAJVRP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.134"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..2887
                     /locus_tag="OQN12_003598"
                     /pseudo
     CDS             1..2887
                     /locus_tag="OQN12_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OQN12_003599"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="OQN12_003599"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS1655786.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="OQN12_003600"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="OQN12_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OQN12_003601"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="OQN12_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="OQN12_003602"
     CDS             complement(7280..8041)
                     /locus_tag="OQN12_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCS1655787.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="OQN12_003603"
     CDS             complement(8038..8697)
                     /locus_tag="OQN12_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1655788.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2319 g         2047 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggtt
//
LOCUS       DAJVRP010000135         8647 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVRP010000135 DAJVRP010000000
VERSION     DAJVRP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.135"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(769..1965)
                     /gene="hemY"
                     /locus_tag="OQN12_003604"
     CDS             complement(769..1965)
                     /gene="hemY"
                     /locus_tag="OQN12_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1655789.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1968..3185)
                     /gene="hemX"
                     /locus_tag="OQN12_003605"
     CDS             complement(1968..3185)
                     /gene="hemX"
                     /locus_tag="OQN12_003605"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1655790.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(3207..3947)
                     /gene="hemD"
                     /locus_tag="OQN12_003606"
     CDS             complement(3207..3947)
                     /gene="hemD"
                     /locus_tag="OQN12_003606"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1655791.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3944..4885)
                     /gene="hemC"
                     /locus_tag="OQN12_003607"
     CDS             complement(3944..4885)
                     /gene="hemC"
                     /locus_tag="OQN12_003607"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1655792.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5272..7818
                     /gene="cyaA"
                     /locus_tag="OQN12_003608"
     CDS             5272..7818
                     /gene="cyaA"
                     /locus_tag="OQN12_003608"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1655793.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7858..8178)
                     /gene="cyaY"
                     /locus_tag="OQN12_003609"
     CDS             complement(7858..8178)
                     /gene="cyaY"
                     /locus_tag="OQN12_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1655794.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8226..>8647)
                     /locus_tag="OQN12_003610"
     CDS             complement(8226..>8647)
                     /locus_tag="OQN12_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCS1655795.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1968 a         2235 c         2225 g         2219 t
ORIGIN      
        1 agttgacggg gtatctgctg cgctggccat gagaggaaga gcggcggcga cagcaacaac
       61 aagacgtttg ggtgaaaacg aaaattccat gcaaaatgct ccggtttcat gtcatcaaaa
      121 tgatgacgta attaagcatt gataattgag atccctctcc ctgacaggat gattacataa
      181 ataatagtga caaaaataaa ttatttattt atccagaaaa tgaattggaa aatcaggaga
      241 gcgttttcaa tcctacctct ggcgcagttg atatgtaagg caggtttatt atatcgcgtt
      301 gattattgat gctgttttta gttttaacgg caattaatat atgtgttatt aattgaatga
      361 attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat tcactcagga
      421 agttattact caggaagcaa agaggattac agaattatct cataacaagt gttaagggat
      481 gttatttccc gattctctgt ggcataataa acgagtagat gctcattcca tctcttatgt
      541 tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg cttatcgtcc
      601 actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga gtgaagcacc
      661 cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca ggcgtttttt
      721 tatgcatcag aaaggtcccg gaggtgcttt cctccgggtg agaaggaact actgtggcgg
      781 gttattctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt cttccggctt
      841 gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt
      901 cagcgccgcg cggaaggcga gcgatgcttc ctgccattca ccgtgcttca tcagtgactg
      961 acccaatgtg ctccacaaca gcgggcgatc accgacgttt ttgatttgct ggcgtagcac
     1021 tttttccagc tgttccgggt tattggtttt cagacgcggg atcggcagca gtaggcgatc
     1081 gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg gcagtatcat gatcgtcaca
     1141 ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg ttttccggct
     1201 ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg cctgatccat
     1261 caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac caacatgggc
     1321 tttcgccatt gatgggatga tatccagcag agaactccat gcccctgtgc ggatatacgc
     1381 ctgttccgcc agacgtaata cttcaggatg gcgtggcgta acttccagca gcttatccac
     1441 gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac gggtgatttc
     1501 taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt ggttggcgcg
     1561 tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca ccaccggttg
     1621 ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat caccttccgc
     1681 cagtttcagc agcgcctgtt cggtctgctt acgcgcacgg cgacgcttac gtccgacaaa
     1741 ccatccacgg gtgtgcgcgc cagtgcggaa gatccgccgc agtagccact caatggcaaa
     1801 cagcactacc atcgccagaa tcaatatgat cgccaggccc gtgacgctgg tttcgatatt
     1861 gtagttgtcg gtctggatca gcacataacc ctgatgaccg gcaatcatcg ggccaaccac
     1921 tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta ttctccttgc
     1981 ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc aggtgcaggt
     2041 gcaggtgcag gtgcaggttt agcttccgtt gtccccgctg ccggttgtgc cagcaggtta
     2101 cgcacgcgag tctgcatcag tttttccagc atcgcctggc tttgcagggt ttccggaaga
     2161 tccatcgaga tattttgctg gcttaactgg tccacctcgt cgaggaacgc tttggtggtg
     2221 gcatcatcag tatcgtagta agcacgtacc caggtggaga cgttctccag cgcctggcga
     2281 taagtctctt cctgatgacg cggtacagct tgtgctgcga ccagcaggcg agagcgaata
     2341 ttttcgcgca gatagatatc ctgatttggc gctaacagcg gtacggcggt gtcatcacga
     2401 cggcgaatcg taatgaagtt gtccataaag ttctgccagc ttttttgcag attgatacgc
     2461 cattcgctga tggaactgga aagctcttca ccgtctgaat ccatcggcga accatcgctg
     2521 tcattatcgg ccagacgcag gttatctacc tgatttgaaa gctgattaag cttaaggatg
     2581 atgccgtcat aatccacctg cgatactgca gaaaggctgg cgatatcatc ggtaattgcc
     2641 cgacgaacgg taatcagact cgggtcattc atatccgcca ggctggcgtc tgcacttttc
     2701 agcaacgctg cagaggtcgt gacgtcctga tcgctccaca gcttccgtcc ggcgagtttc
     2761 accagaaaat cggcctgagc cagcagccag gttttagcat cgctgccgga aatggtggcg
     2821 atcttttgtt ggacttcatc caactgtttt gccagcgttt cttgctgacg attcgcctgc
     2881 ttaagctgcg cagcttgttg cttaataatg ccttccagct cggctttttg gctctcctgg
     2941 gctttttgca atgccgtcag ttggttagcc agggcatcgc tggtggcggt ctgattgacg
     3001 gcctgttgtt taccccagcc atacaaaccg acacccgccg ccagagcaat agcgatagcc
     3061 accgcgctga gaatcaatgc agtattgttc ttactctttt tttctgttgc gacaggttgt
     3121 gacgtggtgt ccacggcctc cctggtctct tcaaccacgg cggaggtttt ttcttgttcc
     3181 gtcattatgg cttcctgtta tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca
     3241 gcgttatcgg cgaccttaat gtcttgccag cccagttccc gggcgagttt cgccaaacgc
     3301 tcactgacga ccaatagtcg acagtgtagt aaccagtgct cacgatacca ttgtgggatc
     3361 agcgaccaga gttgctgcaa catttcaccg ctggtaacaa cgaccatcgt cacctcgcgg
     3421 gattgccagc gcatcgcttc ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat
     3481 tcacaaaaag tgacctcagc accgcgcacc gtcagggtat ccccaattag ctcacgaccg
     3541 ccattgccac gtaatatcag cgcacgtttg cccgcaatat tttgtaattc aggtaattgt
     3601 agcaagactt cgctgatttc ccgatcctgt gggtagagaa tcttctgtcc gcttacggta
     3661 tgtagcgcca gtgcggtggt gcgtccaatg gcgaaataag taggtagttg gggccattta
     3721 cgatcttgct gatgcagctg tgattgggca aaagcaaccg cgtgttgcga gagggcaaac
     3781 aacagatcgc tctcccccag cgctgccagt tgatcagcaa gttgcggtaa ttgtcgaccc
     3841 ggagaaaact caatcagcgg aaaatgccag gccacctgcc ccagtgtgcg cagacggctc
     3901 actaactctt ctccagcggg agacgggcgg gtgaccagga tactcatgcc ggggcctctc
     3961 cgttatagac ttcagcgagg atctcgcgcg cgccgttatt cagtagctct tctgccagcg
     4021 aaatccccat ttgttcggca tcttgcggcg caccgcggcg ttcaccgcga ataatctgcg
     4081 aaccgtccgg cgcgccgacc agcgcacata gccagatttc gccatcaata agctcggcgt
     4141 agctaccaat tggcacctga catccgcctt cgagacgggt attcatggcg cgttctgcgg
     4201 taacgcgcag tgcagtttcg tggtgattca gcgcggcaag cagctcgcga gtgcgtgtat
     4261 catcaaggcg gcattcaata ccgactgcac cttgtcctac cgccggaaga gaaatctcgg
     4321 gtggtaacgc ggcgcgaatg cgggactcca gacctaaacg ttttagtccg gctacggcaa
     4381 gaatgatggc atcgtattcg ccgttatcta gtttgctcag gcgagtgccg acgttgccgc
     4441 gcagggagcg gataatcaga tccgggcggc gttcagccag ttggcactgg cgacgtaaac
     4501 tggacgtccc gacgatactg cctgctggta acgcatccag actgtcatag ttattggaca
     4561 caaaggcatc gcgaggatct tcacgctcgc aaatagtgac cagccccaga ccttgcggga
     4621 attcaaccgg cacatctttc attgaatgta cggcgatatc agcgcgattc tccaggagcg
     4681 cgacttccag ctcttttaca aataagcctt ttccgcctac tttcgccagc ggcgtatcaa
     4741 gaatcacatc gccgcgcgtc accatcggta ccagttcaac gaccaggccc ggatggctcg
     4801 ccatcaactt gtctttgaca tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg
     4861 caattcttaa aacattgtct aacatgcttg ttaccgtcat tatcatccgt ggtccatcct
     4921 aacatccttg ccagagtgat gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt
     4981 aaaatgaggt ctggcagtgg atcctgacag gcgtttcacg ccgtcgtaat aaggaattta
     5041 cagagaataa acggtgctac acttgtatgt agcgcatctt tctttacggt caatcagcaa
     5101 ggtgttaaat tgatcacgtt ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg
     5161 cgaaaagtgg taacggttac ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt
     5221 tgatgttggc ggaatcacag tcatgacggg tagcaaatca ggcgatacgt cttgtacctc
     5281 tatattgaga ctctgaaaca gagactggat gccataaatc aattgcgtgt ggatcgcgcg
     5341 cttgctgcta tggggcctgc attccaacag gtctacagtc tactgccgac attgttgcac
     5401 tatcaccatc cgctaatgcc gggttacctt gatggtaacg ttcccaaagg catttgcctt
     5461 tacacgcctg atgaaactca acgccactac ctgaacgagc ttgaactgta tcgtggaatg
     5521 tcagtacagg atccaccgaa aggtgagctt ccaattactg gtgtatacac catgggcagc
     5581 acctcgtccg tagggcaaag ttgttcctct gacctggata tctgggtctg tcatcaatcc
     5641 tggctcgata gcgaagagcg ccagttacta caacgtaaat gcagcctgct ggaaagctgg
     5701 gccgcctcgc tgggtgtgga agtcagcttc ttcctgattg atgaaaaccg cttccgtcat
     5761 aatgaaagcg gcagcctggg gggcgaagat tgtggctcca cccagcatat actgctgctt
     5821 gacgaatttt atcgtactgc cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg
     5881 ccgtgcgacg aagaagagca ttacgacgac tatgtgatga cgctttacgc gcagggcgtg
     5941 ctgacgccaa atgaatggct ggatctcggt ggcttaagct cgctttctgc tgaagagtac
     6001 tttggtgcca gcctttggca gctctacaag agtatcgatt ccccatacaa agcggtgctg
     6061 aaaacactgc tgctggaagc ctattcctgg gaatacccga acccacgtct gctggcgaaa
     6121 gatatcaaac agcgtttgca cgacggcgag attgtatcgt ttggcctcga tccatactgc
     6181 atgatgctgg agcgtgttac tgaatacctg acggcgattg aagatttcac ccgtctggat
     6241 ttagtacgtc gctgcttcta tttaaaagtg tgcgaaaaac tcagccgtga acgcgcctgt
     6301 gtaggctggc gtcgcgcagt gttgagccag ttagtgagcg agtggggttg ggacgaagct
     6361 cgtctggcaa tgctcgataa ccgtgctaac tggaagattg atcaggtgcg tgaggcgcac
     6421 aacgagctgc tcgacgcgat gatgcagagc taccgtaatc tgatccgctt tgcgcgtcgc
     6481 aataacctta gcgtctccgc cagtccgcag gatatcggcg tactgacgcg taaactgtat
     6541 gccgcgtttg aagcattgcc aggtaaagtg acgctggtaa acccgcagat ttcacccgat
     6601 ctctcggaac cgaatctgac ctttatttat gtgccgccgg gccgggctaa ccgttcaggt
     6661 tggtatctgt ataaccgcgc gccaaatatt gagtcgatca tcagccatca gccgctggaa
     6721 tataaccgtt acctgaataa actggtggcg tgggcatggt ttaacggcct gctgacctcg
     6781 cgcacccgtt tgtatattaa aggtaacggc attgtcgatt tgcctaagtt gcaggagatg
     6841 gtcgccgacg tgtcgcacca tttcccgctg cgcttacctg caccgacacc gaaggcgctc
     6901 tacagcccgt gtgagattcg ccatctggcg attatcgtta acctggaata tgacccgaca
     6961 gctgcgttcc gcaatcaggt ggtgcatttt gatttccgta agctggatgt cttcagcttt
     7021 ggcgagaatc aaaattgcct ggtaggtagc gttgacctgc tgtaccgcaa ctcgtggaac
     7081 gaagtgcgta cgctgcactt caacggcgag caatcgatga tcgaagccct gaaaactatt
     7141 ctcggcaaaa tgcatcagga cgccgcaccg ccagatagcg tggaagtctt ctgttatagc
     7201 cagcatctgc gcggcttaat ccgtactcgc gtgcagcaac tggtttctga gtgtattgaa
     7261 ctgcgccttt ccagcacccg ccaggaaacc gggcgtttca aggcgctgcg cgtttctggt
     7321 caaacctggg gcttgttctt cgaacgcctg aatgtatcgg tacagaagct ggagaacgcc
     7381 atcgagtttt atggcgctat ttcgcataac aaactgcacg gcttgtcagt gcaggttgaa
     7441 accaatcacg tcaaattacc ggcggtggtg gacggctttg ccagcgaagg gatcatccag
     7501 ttctttttcg aagaaacgca agacgagaat ggctttaata tctacattct cgacgaaagc
     7561 aaccgggttg aggtgtatca ccactgcgaa ggcagcaaag aggagctggt acgtgacgtc
     7621 agtcgcttct actcgtcatc gcacgaccgc ttcacctacg gctcaagctt catcaacttc
     7681 aacctgccgc agttctatca gattgtgaag gttgatggtc gcgaacaggt gattccgttc
     7741 cgaacaaaat cgattggcaa cctgccgccc gccaatcagg atcacgatac gccgctatta
     7801 cagcagtatt tttcgtgatg agcgtgccgg aaagcgaggc ttatccggca tgcaatctta
     7861 gcggaaactg actgtttcac ccgcctgttg cgtcgccgcc tgttccagca aatcccagaa
     7921 ggtttcgccg ctgcgatcgc aaatccattc atcgcctttc aggtcaaaat ggtagccgcc
     7981 ctgtttggtt gccagccaca cctggtgcag cggctcctgg cggttaataa tgattttgct
     8041 gccgttttca aaggtaatgg tcagtacgcc gccgttgatt tcgcagtcga tatcgctgtc
     8101 gccatccctg tcgtccagac gctcttcaat ggtcagccac agttgatcag ccaggcgatg
     8161 aaattcactg tcgttcattg ttgtatcctg tttttaagtg atggcggcag tatagcggca
     8221 tggggtcagg gcttcaaagt ttgcacctct gctgctgcgt tccggcatga ttcatccgtc
     8281 accggaataa tgatgtctct gttttgaact tcacgtacgt tctcctctct gacacgagtt
     8341 ttcgccgggc agggggaggg cgtaatcctt acctctgtat cggtccatgt ataaacattg
     8401 cgatcgtcat aagcgaagcg atcgttttta gttagcgaac cgttaactgg aataatttgc
     8461 aagctctttg accgtaattg gcggataaag taagcatgct gtttgccgct attaaatgag
     8521 cgatcaatga ccaggtctgg cgtatccagc gggattatct caagctggtc ttcaccaaat
     8581 gtcgataatt ttgttacata cagccgatct ttatattgcg caatattgcc agtaatcaga
     8641 tggaatt
//
LOCUS       DAJVRP010000136         8567 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVRP010000136 DAJVRP010000000
VERSION     DAJVRP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.136"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            94..1386
                     /locus_tag="OQN12_003611"
     CDS             94..1386
                     /locus_tag="OQN12_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1655796.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1540..3189)
                     /locus_tag="OQN12_003612"
     CDS             complement(1540..3189)
                     /locus_tag="OQN12_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1655797.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(3340..4689)
                     /gene="ghxP"
                     /locus_tag="OQN12_003613"
                     /gene_synonym="yjcD"
     CDS             complement(3340..4689)
                     /gene="ghxP"
                     /locus_tag="OQN12_003613"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1655798.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(5395..5859)
                     /gene="soxR"
                     /locus_tag="OQN12_003614"
     CDS             complement(5395..5859)
                     /gene="soxR"
                     /locus_tag="OQN12_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1655799.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5945..6268
                     /gene="soxS"
                     /locus_tag="OQN12_003615"
     CDS             5945..6268
                     /gene="soxS"
                     /locus_tag="OQN12_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS1655800.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(6271..7848)
                     /gene="pdeC"
                     /locus_tag="OQN12_003616"
                     /pseudo
     CDS             complement(6271..7848)
                     /gene="pdeC"
                     /locus_tag="OQN12_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            8277..8558
                     /locus_tag="OQN12_003617"
     CDS             8277..8558
                     /locus_tag="OQN12_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCS1655801.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2441 a         2147 c         1919 g         2060 t
ORIGIN      
        1 aactcattaa aaaatgagcg atttttgaca gtcgtagaaa atgataatgc agagaatatg
       61 ccttttcttt cttgttaatt ataaggatat tttatgcgct ataatggttt aaataatatg
      121 tttttccctc tttgccagat taacgataac cactctatca caagttcatc acatacaaag
      181 aaaacaaaat cagataatta cagcaaacat cataaaaaca cgttaattga caataaagcc
      241 ctctctcttt tcaaaataga tgatcatgaa aaagtgatag gcttgattca gaaaatgaaa
      301 agaatttatg atagtttacc atcaggaaaa atcacgaaag aaacggacag gaaaatacat
      361 aaatatttta tagatatagc gttatatgca aataataaat gtgacgatag aattacgaga
      421 agagtttacc ttagtaaaga aaaggatgta tccattaagg tggtatattt tataaataat
      481 gtcgccgtcc ataataatac tatcgaaatt ccacagatag taaatggtgg ttacgatttt
      541 tcacacctta gcctgaaggg tatcgtgatt aaagatgaag atttatccaa ttcgaatttt
      601 gcaggttgca gactacaaaa cgctattttc caggactgta atatgtataa aacgaatttt
      661 tattacgcca taatggaaaa aatacttttt gataattgta ttctcgatga ctcaaatttc
      721 gctcagataa aaatggccga cggaactcta aatgcatgct ccgctatgca tgttcaattc
      781 tacaatgcag caatgaatag agccaatatt aaaaatacct tccttgacta ttcaaatttt
      841 tatatggcgt acatggctga ggtaaatctt tataaagtaa tagcgccata tgttaattta
      901 tttaaagccg accttagttt ctctaaactc gatttaatta actttgaaca tgctgatctg
      961 tctcgcgtca atctgaacaa agcaatcctc cagaatataa acttaattga tagcaaactc
     1021 ttttgtacgt ggctgacaaa tacattcctc gaaatggtta tatgtaccgg ctctaatatg
     1081 gctaatgtta attttaataa tgccaattta agcaactgcc atttcaactg ttctatttta
     1141 acaaaagcct gtatgtttaa tacccgtctc tatcgggtta atttcgatga ggctagcgtc
     1201 cagggaatgg gcatttccat tctccgtggg gaggaaaata tccccattga tagtgatacc
     1261 ctggtaacac tacagaaatt ctttgaagaa gattgtacct ctcatactgg catgtcacaa
     1321 actgaggata atattaatgc agtcgctatg aagattactg cagatattat gcaacacgca
     1381 gattgaggtt aagtcgaaga acagccaatg aattgacttt acaagagaga taacttattt
     1441 gttttagaat catgaatgtt cgcccgagct aacctgttaa aggtgcaaaa gattgcacct
     1501 ttttcagatt acctcgggcc tggttacgtc gctttcggct tactgatttt cctcaattag
     1561 caacgcttca agcaaatcga gatcgtgcag taatttttgc agcgtttcgt tgctgatctc
     1621 ccgcgtggcg cgcaggtggt acagttcagc acgttcagaa cgcaatgccg ccagacggaa
     1681 gcgacgctca aggttctctt cctgcacgga actttcaacg tcgttacgtc catcggcgcg
     1741 acgacgcagg ttaccaatga cgcgagaact gacctccgta agcaactggt tatcgatatt
     1801 ctcttcggta tccgccgcca gacgctcctc cattttctgg atcgccacaa tcgccacttc
     1861 tgccgttgcc gctcgcgcaa tacgttcttc tttcaattgt tgcgaatggt ccgcgacttc
     1921 aatgtgttgt agcagaatag gcaacatcac cacgccgaca aacagcgaga agagaatgac
     1981 gccagccgcc aggaacacca gctcatagcg cgccgggaag acgttaccat ccggcaagag
     2041 cagcgggatt gagagcacac cggccagagt gatcgcccca cgcaccccag cgaaagacgc
     2101 gatcaggatt tctcgtgtgg tccacgaacc aaactccatc ggcttcttct tcaggaagcg
     2161 gttgctgaac tttttcatcg tccacagcca gccgaaacgg accagcatca gcgccgcata
     2221 tatcagaata atatcggtaa acagcatcca gatttcgacg ttagggtcga tttctgccgc
     2281 catcagcgac gtctccagaa tacccggcag ttgcagacct aacagcagga acaccatgcc
     2341 gttaaagaca aattccagca tcgcccaggt gctgtttgcg cgcaggcgca ttgccagtgg
     2401 cgcacggcgc atcacaccgg agcgggtgat ggtcatcccg gcggcaaccg cagcgaggat
     2461 gcccgaaacg ccaatatgtt cggcaatcag ataagaagcg aatggcagca agaacagcag
     2521 aacgatctgc gtcgcgggtt catcaccgcc ccagcggctg aggaatcgca gcgagcgacc
     2581 atacagccag ctcaccacaa aaccggcaag aataccgcca atggctactt tcataaattc
     2641 gaccgtcgca ccgccgacag tgaagatcat cgtccccatc gccactgcca cggcaaactt
     2701 caacgacacc aggccggaag cgtcattcat caacgcttcg ccctgcaaaa tgcccatgat
     2761 ttttttcggg atgcgccctt cgccaacaat cccggagagc gccacagcat cggtcggaga
     2821 aagtaccgcc gccagcgcaa aggcggggat cagcggaatg cccggcacca cccagtaaat
     2881 aaggaagcca atgccgacca cggtgaccac caccagcgcc agcgcgaggc cgaaaatctc
     2941 tcgaccatgt tcgagaaatt cacgggtcgg cgttttccag ccatcagcga acagcaacgg
     3001 cgggataaac aagactaaaa agagttcagg atcaaactcc acatgcaaac caaacgtcgg
     3061 ccacgccagt agcgcaccga tggcgatttg cataagcgga agcgggatct gaaagggcat
     3121 gacacgagtg accaccccgg acagcgagac cacgagggtc attatcagta tggtgaagaa
     3181 gatttccatg cgttccctgt ttgcgatgtt ttttatgatc tgagaaagac gttatgcctg
     3241 aattctcatt ttttcagaat atcgcaaaag ctcacacaat gtgcggagaa aataaaaacg
     3301 ggcagccatt gccacccgtt ttcagaatcc gatgaaagat tagatagccc agccacccgc
     3361 atagaaggtc accagcgcca cggcgataac gaccgtaccg atgttcaact tgcgccattc
     3421 accggaaacc agacgaccaa tcaccagagt cgcgaagccg atcatgatgc ctgttacgat
     3481 gttacaggtc agcacgatga ataccgccgt aaccagaccc gccatcgcat caacaaaatc
     3541 agcaaagtcg attttcgcca cgttgctcag catcagcagg ccaacgtaca tcagcgccgg
     3601 agccgttgca taccccggaa cgaggtaaga aagcggagag aggaacagaa tcagcaggaa
     3661 cagcacgcca acggtgatag ccgtcaaccc ggttttaccg cccgccgccg tacccgccgc
     3721 agactcgatg tataccgctg ccggagctgc acccaccagg ccagagaaaa cgctgctcat
     3781 ggagtcagtg gtcagtgctt tcccgccgtc gatgatctgc ccatctttat ccagcaggtt
     3841 cgcctggccg gcgacagcgc ggatagttcc ggtggcatca aatactgccg tcatcaccag
     3901 cgccagaacg cttggcagga ctacaggatt cagcgcgccc ataatgtcca ggctgccaat
     3961 cagtgaattg ccgttttcat cgctcaatga aggcatggcg aaaacgccgg agaaatggac
     4021 gttaggatcg aagatcaaac cgacaattga gataccgata atggtcagca gaatgccacc
     4081 agggactttc agtttttcca agccgatgat caccgccaga cctaccagtg acataatcac
     4141 cgggaaggtc gcgaaatcac ccagcgcaac gggcagacca tcaagcgggt ttttaatcac
     4201 cagaccgaca ccattagcgg caatgagcag caggaacaga ccgataccga tccccgtgcc
     4261 gtgcgccaca ccgtgcggca agttgcgcaa aatccagcta cggatacccg tggcagaaat
     4321 taccgtaaac agcacaccca tcaggaacac ggcacccagc gcgacaggta cgctaatatg
     4381 ttgccccagc accaggctga atgcggtaaa cgccgtcagg gagatggcgc aaccaatcgc
     4441 caacggcaga ttagcccaca gacccatcac gatagaaccg agtccggcaa ccagacaggt
     4501 tgcaacgaaa actgccgcag gcgggaagcc tgctttaccc aacatacctg gaacgacgat
     4561 gaccgagtag accatcgcca gaaacgttgt taacccggca accacttcct gacggacagt
     4621 gcttccacgt tgtgaaattt taaaccaggc gtcgagtgaa ccgccggtac gcgctgatgg
     4681 cgtagacata agaaaacatc ccctgagagt ttaattttcg tcaagatgcg tggcggacaa
     4741 tcccctgcca gcgaaacgtt atcttcctgc tccaggtttg cggcaatttg gtgcgtcgca
     4801 aaaaagcaaa cgtttagctc cgcgcataca aaacgggtgg taaaaaaaag caaacgatta
     4861 tccggcgatc atagcggctt tttcaaccaa agtttgctgc ttttttctct tttattgtct
     4921 tgacgatgaa aaaccaggca attgatgcgc aaacgttatc gtcgatgtgc aagattgcag
     4981 cgttatcgag ccgtataccg ttctggattg cgataaaaaa tcgtctgaga tgacaaaaat
     5041 attgcggcta aaaagtatac ttttacactg gcgaagaaac gtcggtgaca tacaattaaa
     5101 gcatcaacac caaccggaac ctccaccacg tgctcgaatg aggtgtgttg acgtcggggg
     5161 aaaccctcct gtgttccagc gggatagaga gaaagacaaa gaccggaaaa caaactaaag
     5221 cgcccttgtg gcgctttagt tttgtgtttg cggataattc caaaagagct cgatcagtcc
     5281 cctcgcccct tcggggagag ggttagggtt aggggaacag gcagcaccag tgcaaatatt
     5341 taccctcacc ccggccccat agttaacgat gagtcagctt gtccttgtgg cgctttagtt
     5401 ttgttcatct tccagcaagc gtgcgccggt accttcttct cctaagcggt cgcccgggtt
     5461 acgcaacggg caatcactgc gcgaaaggca gccacaacca atacatccgt ccagttcgtc
     5521 acgcagcgcc actaaggtat gaatgcgccg atccaactct tctcgccatt gggacgaaag
     5581 ctgtttccac tctttcgcac ttaacgtatg cccttctggc aacacgccaa acgcttcacc
     5641 aatggtcgcc agcggaatgc caatacgctg agcgattttg ataattgcga catatcgcaa
     5701 cacatcacgt ttatatcgcc gctgattgcc gctgttacgg atactagtaa tcaacccttt
     5761 gctttcatag aaatgcagcg ccgataccgc cacaccgctg cgtttcgcca cttcgccagg
     5821 ggttagcagc gctttaatgc ggggtaattt cttttccata aatcgcttta cctcaagtta
     5881 acttgaggaa ttatactccc caacagatga attaacgaac tgaacactga aaagaggcag
     5941 atttatgtcc catcagaaaa ttattcagga tcttatcgca tggattgacg tgcatattga
     6001 ccagccgctt aacattgatg tcgtcgcaaa aaaatcaggc tattcaaagt ggtacttgca
     6061 acggatgttc cgcacggtga cgcatcagac gcttggcgat tacattcgcc aacgccgcct
     6121 gttactggcc gccgttgagt tgcgcaccac cgagcgtccg atttttgata tcgcaatgga
     6181 cctgggttat gtctcgcagc agaccttctc ccgcgttttc cgtcggcagt ttgatcgcac
     6241 tcccagcgat tatcgccacc gcctgtagtt ttattgcccg cgcgttaact cccgcgcggg
     6301 taattgctcc atccattgca taaacacctg cggcggcatc gccttcgcaa aaaaccatcc
     6361 ctggcaatag cgcacgccgc gtttgcgcag ccagttaacc tgctcctcag tttcgacgcc
     6421 ttcagcgatc gtttttaacc ccaggctgtg cgccagctcg atgatgtgtt ccgcaatcaa
     6481 atgactggtt ttgtgggtgg tcagcgtttc aacaaacgat ttatcgattt tcaaaatatc
     6541 gacattcaat gatttaaggt tatgcaagtt agagtagcca ataccaaaat catcaattgc
     6601 cacttcgtaa ccagcctggc ggaaagccag aattaatcgg cgtcattttt tcgacatcaa
     6661 gaaatgcatg ctcagtcact tcaaatttaa tttgctgcgg acgcaccgcg tattgctctg
     6721 ttttctgatt gattcgcgct atcaaccgtg acgtatgaaa atcggaggcc gacaggttaa
     6781 tagaaacata gcgatcggca tgtgttgcca ggtacgcgcc cagatcgcgg aagacattat
     6841 caataacata atcagttatc tgttctatca tcccctcctt ttctgccagc ggaataaact
     6901 ctgccggatt cattatttgc ccctgctcac caggccaacg taacaacgct tcagcgccga
     6961 tacatttttc tgttttgata tcgattattg gctggtaata aagacaaagt tgatgttttt
     7021 cgagggcgcg ttgcaattta cgtttgggag ataaatagtt ttgtcgaata cgtagccaga
     7081 gtaatagcag aacaagactc cccaaaatac cggcgggcaa cgcaaaaata agatgattat
     7141 aaaaatgggt tataagacgt tgatatgaag tcgcaacaat ggctgcaatt gggcgttttg
     7201 tcgaataaat tgtcgcatat aaatagccat ttctttgtac ggttaaatcc ttcaaatgaa
     7261 tcagcggaga aaacgttgct gccgaggcct ctttgcttaa cgagaaaaag gttttcgtca
     7321 ccgtatcata cacaccccat tgcaatgtcg ggtcatcaga catcacttca ctccagaaga
     7381 gagggttgat aaccgccaca taatttcccc gctgcatata ggtcatttta tagccagaga
     7441 aaaaaggcgt atcgcggtaa taatagatag aaacgttagg ttcacgctta taatcggccg
     7501 gtgcaatcgt atagccgttt acaggcgcta tcagcgatga gcataaaaaa tggttatcac
     7561 gaattcatta atataaagat agccacgaat aatattcaac attcgctttt gatgggctgg
     7621 agtgcaaact tgcccctgat agcgttcagc tgcatcgctc actaaatctg cctggcgaat
     7681 gaccagctcg gatttatcca gtgccagttg agcaaaggtt cggagctgat tattcacttc
     7741 tgattttgcc caaaggaagg caatccatag cgaaagaatg atgggaaaga gaactaaaaa
     7801 gataatgccc ggcaacgcca gtaattggtg tcgtgcacga tgactcattg ctatcaattt
     7861 cctgcctgtt tatacccagc cattgcaagg tgaaaccctg gctaccgacg tctttagata
     7921 aatcttacgg tataaatagc acttttgcct gatttgaaat tgataccttt ttaatcgtca
     7981 cctggatgaa aataattcat tgatattcaa aattaatttt ctacaggaat acagaggcgg
     8041 cgggaagata tgcccttaat gccactgaaa aaccacataa tttgtacagc aataaaagtc
     8101 acggcctaat atggctaaca aacaatcaat tgtgatatag ttcacaaaat taatgaaaca
     8161 aacagagtgt ttcatttttg tgttctgcaa gccccgcgat agccagagtg taccacgcct
     8221 cccgtgaaca acgccgcgct gtccagggca tcggctcttt tcagaggaat tgtttgatgg
     8281 ctaccctcac cactggcgtg gttcttcttc gctggcaact tcttagtgcc gtaatgatgt
     8341 ttctggccag cacactcaac atccgttttc gtcggtctga ttatgtcggg cttgcggtga
     8401 tcagcagcgg tctgggcgtt gtttccgcct gttggttcgc aatggggttg cttggcatca
     8461 caatggcgga catcaccgct atctggcaca acatcgagtc ggtgatgata gaagagatga
     8521 atcagacacc gccacaatgg ccaatgattt tgacctgata cgtagaa
//
LOCUS       DAJVRP010000137         8412 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVRP010000137 DAJVRP010000000
VERSION     DAJVRP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.137"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OQN12_003618"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OQN12_003618"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1655802.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OQN12_003619"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OQN12_003619"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCS1655803.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OQN12_003620"
     CDS             3115..3399
                     /locus_tag="OQN12_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1655804.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OQN12_003621"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OQN12_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1655805.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OQN12_003622"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OQN12_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1655806.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OQN12_003623"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OQN12_003623"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1655807.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2019 a         2253 c         2139 g         2001 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gc
//
LOCUS       DAJVRP010000138         8384 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVRP010000138 DAJVRP010000000
VERSION     DAJVRP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.138"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..805
                     /gene="ttdA"
                     /locus_tag="OQN12_003624"
                     /pseudo
     CDS             1..805
                     /gene="ttdA"
                     /locus_tag="OQN12_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            802..1407
                     /gene="ttdB"
                     /locus_tag="OQN12_003625"
     CDS             802..1407
                     /gene="ttdB"
                     /locus_tag="OQN12_003625"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1655808.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1455..2918
                     /gene="ttdT"
                     /locus_tag="OQN12_003626"
     CDS             1455..2918
                     /gene="ttdT"
                     /locus_tag="OQN12_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1655809.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2961..3974)
                     /gene="tsaD"
                     /locus_tag="OQN12_003627"
     CDS             complement(2961..3974)
                     /gene="tsaD"
                     /locus_tag="OQN12_003627"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1655810.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4212..4427
                     /gene="rpsU"
                     /locus_tag="OQN12_003628"
     CDS             4212..4427
                     /gene="rpsU"
                     /locus_tag="OQN12_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1655811.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4538..6283
                     /gene="dnaG"
                     /locus_tag="OQN12_003629"
     CDS             4538..6283
                     /gene="dnaG"
                     /locus_tag="OQN12_003629"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1655812.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6478..8319
                     /gene="rpoD"
                     /locus_tag="OQN12_003630"
     CDS             6478..8319
                     /gene="rpoD"
                     /locus_tag="OQN12_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1655813.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2022 a         2252 c         2249 g         1861 t
ORIGIN      
        1 ttgacagaga ttgtcgctaa ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg
       61 gtggataaac taaaacagct aaaggatgcc gaaacgtcgt cgatggggaa aattatctac
      121 catacgatgt tcgacaacat gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac
      181 accggggaga ttatgttctt cgttaaagtc ggttcccgct tcccactgct tggcgagctg
      241 caaagcatac tcaaacaagc cgtggaagag gcaaccgtca aagcgccact acgtcacaat
      301 gcggtagaaa tttttgacga agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg
      361 gtcacctggg acatcatccc cgacaatgac gatgcggaaa tcgaagttta catggcaggc
      421 ggcggctgca cgctacctgg ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc
      481 gtggtgaaat tcgtcttcga aaatatctcc accctcgccg taaacgcctg tccaccggta
      541 ctggtgggcg tgggcatcgc cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc
      601 attttgcgcc cgattggcag ccgccatccc aatccaaaag cggcagaact ggagctacgc
      661 ctggaagaag gactcaaccg tctggggatt ggtccacaag ggctgaccgg caacagttca
      721 gtgatgggcg catcgtcgcg gcacgctgct ggttcatgcc gatctcacct ttgaaaatct
      781 gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat
      841 ctgcaagata ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgc
      901 gacgtttgtc accgccgttt gatcgaactg aaacgtccga tcccttacga tctcaacggc
      961 aaagcgattt tccacgctgg ccccatcgtg cgcaaaaacg gtgacaaatg ggagatggtc
     1021 tccgtcggcc cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tattgagcag
     1081 accggcgtaa aactggtggt tggcaaaggt ggtatggggc cgctgaccga agaaggctgc
     1141 cagaaattca aggcgttaca tgtgattttc ccagcaggct gcgcggtgct ggcggcaacc
     1201 caggtagaag agattgaaga agtgcactgg acagaactcg ggatgccgga gtcactgtgg
     1261 gtctgccggg tcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaac
     1321 ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc
     1381 tgcgagcacg tccattacat caaataaccc tcccggagag gctcacccct ctcctttttc
     1441 gcaggcataa cacgatgaaa ccttccactg aatggtggcg atatcttgcg ccgctggcgg
     1501 tcatcgccat tattgctcta cttccggttc ccgcagggct ggagaaccat acctggctct
     1561 actttgccgt ttttactggc gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg
     1621 tggtggcgat ggtgggtatc tccatcatcg ccatactctc tccctggctg ctgttcagcc
     1681 ccgaacagct cgcccagcca ggctttaagt tcaccgccaa atccctctcc tgggcagttt
     1741 ctggtttttc taattcggtt atctggctca ttttcgccgc ctttatgttt ggcacaggct
     1801 atgaaaaaac cgggcttgga cgccgcattg cgctgattct ggtgaaaaag atggggcatc
     1861 gcacgctgtt tctcggctat gcggtgatgt tctccgagct gatcctggca cctgtaacac
     1921 cgtccaactc ggcgcgtggt gcgggtatta tctatcccat catccgtaac ctgccaccgc
     1981 tctatcaatc acaaccaaac gacagcagtt cgcgcagcat tggctcgtac atcatgtgga
     2041 tggggattgt tgccgactgc gtgaccagcg ccattttcct gacggcgatg gcacctaact
     2101 tgctgttaat tggactgatg aaaagcgcat ctcacgccac gctgagttgg ggcgactggt
     2161 tcctcgggat gttgccgctc agcattttac tggttctgct ggttccctgg ctggcttacg
     2221 tgctgtaccc gccggtactg aagtctggtg atcaggtgcc gcgctgggca gagacggaac
     2281 tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg
     2341 cgctggtgct gtggattttc ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg
     2401 tggtggcgct gatgctgctt ctgcgcatta tcagttggga cgacattgtc agtaataaag
     2461 cggcatggaa cgttttcttc tggctggcct cgcttatcac cctcgcgacc ggactcaaca
     2521 ataccggttt tattagctgg tttggcaaac tgttagcagg cagcttaagt ggttattcgc
     2581 cgacgatggt gatggtgacg ttgattgtgg tgttttatct actgcgctac tttttcgcca
     2641 gcgccacggc gtatacctgc gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc
     2701 cggaaatccc gctgccggtg ttctgcctga tggttggcgc ggcaattggt ctggggagca
     2761 ttcttacacc atacgccacc ggacccagtc cgatttacta cggtagtggt tatctgccaa
     2821 cggcggatta ctggcgactg ggggcgattt ttgggctgat attcctcgtg ttgctggtga
     2881 ttaccggctt actgtggatg cccgtggtgt tgctttaagt gaggacggtg ccggaaattg
     2941 ctctccggca cccaatagtt ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc
     3001 acgctaacac cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg
     3061 atcatcgcgc cgttatcagt acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt
     3121 tttttcatca tttcagccag cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc
     3181 atgaccagtc gcttaaagcc cgtctgatcc aacgctcgct tacacttaat catcaacgta
     3241 tcgaccaccg catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg
     3301 ttgtcacgaa tcgtgttcgc cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc
     3361 ggacggtcgg tcatcggacg cgggaaaaca aagcgcccgg cagtaccctg agccgccatt
     3421 ttcgacagta acggtccgcc aggataatcc agccccagca gcttcgcggt tttatcaaac
     3481 gcttcaccgg cggcatcatc gatagactcg ccgagcagct cgtactgacc aatgccagtc
     3541 acgctgatta actgcgtatg accgccggac accagcagcg cgacaaacgg aaattccggc
     3601 gggttatctt ccagcatcgg cgctaacaga tgcccttcca tatggtgtac cgggattgcc
     3661 ggaacgttcc aggcaaacgc cagagaacgc cccacggttg cgccaaccag cagtgcgccg
     3721 actaatccag ggcctgcggt ataggccaca gcatcaatgt cttttgccgt taaaccagac
     3781 tcctttagcg ccgcctggat caacggtacg gttttacgca catgatcgcg ggaggccagt
     3841 tcaggcacga cgccgccgta gtcagcgtgc aatttcacct gactatacaa ttggttggct
     3901 aacaaacctt tttcatcgtc gtaaatggcg atgccggttt catcgcagga agtttcaata
     3961 cccagtacac gcatgacttg ttttacctcg ctttattacc gcgcagtgta ggaccaatgc
     4021 gggttgatgt aaaactttgt tcgcccctgg agaaagcctc gtgtatactc ctcaccctta
     4081 taaaagtccc tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa
     4141 ttccgcacca ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag
     4201 gtgagaggca catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc
     4261 gtcgcttcaa gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt
     4321 tctatgaaaa accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg
     4381 cgaagaaact ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg
     4441 ttgctctccg atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg
     4501 cttattttcg tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt
     4561 attcattaat gatctgctgg cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa
     4621 gctgaaaaag cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc
     4681 gtcattcacc gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg
     4741 caacgcgatc gacttcctga tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga
     4801 gctggcagca atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat
     4861 cgagcgccat cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca
     4921 acaatcttta caacaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt
     4981 aagccacgag gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt
     5041 cctgaagcgg tttggcggca atccagaaaa tcgccagtca ttgattgatg cggggatgtt
     5101 ggtcactaac gatcagggac gcagttacga tcgtttccgc gaacgggtga tgttccccat
     5161 tcgcgataaa cgcggtcggg tgattggttt tggcgggcgc gtgctgggca acgatacccc
     5221 caaatacctg aactcgccgg aaacagacat tttccataaa ggccgccagc tttacggtct
     5281 ttatgaagcg cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat
     5341 ggacgtggtg gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc
     5401 aaccaccgcc gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta
     5461 tgacggcgac cgtgcaggcc gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta
     5521 catgacagac ggccgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac
     5581 gctggtacga aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccact
     5641 ctccgcattt ctgtttaaca gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg
     5701 cgcacgtttg agtacgctgg cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg
     5761 aatatatctt cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga
     5821 acgattaatg ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg
     5881 cacgaccatg cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt
     5941 cccgccgctt gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact
     6001 ggtcaacact tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg
     6061 tggtacaaat aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa
     6121 gaatattgct gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga
     6181 actgcgccag gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg
     6241 cctggagctc tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc
     6301 cgaagagcga tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt
     6361 acgccctcgt aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa
     6421 ggacatcggg tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg
     6481 gagcaaaacc cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat
     6541 ctgacctatg ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc
     6601 gaagacatca tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat
     6661 gccgatgatc tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg
     6721 caggtgcttt ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac
     6781 atgcgtgaaa tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag
     6841 cgtattgaag acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc
     6901 acctatctgc tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg
     6961 atcactggct ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc
     7021 ggttctgagc tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc
     7081 gatgacgaca gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt
     7141 gcggaactac gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt
     7201 cacgctgccg ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg
     7261 gtgccgaagc agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt
     7321 acgcaagaac gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac
     7381 ttcattaccc tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgcg
     7441 atgaacaagc cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg
     7501 caaaaactgc agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc
     7561 aaccgtcgta tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa
     7621 gcgaacttac gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc
     7681 cttgacctga ttcaggaagg taacatcggc ctgatgaaag cggttgataa attcgaatac
     7741 cgccgtggtt acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgt
     7801 tctatcgcgg atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac
     7861 aaactcaacc gtatttctcg ccagatgctg caagagatgg gccgtgaacc gacgccggaa
     7921 gaactggctg aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc
     7981 aaagagccaa tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat
     8041 ttcatcgagg ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt
     8101 gcggcaacgc acgacgtgct ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg
     8161 cgtttcggta tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac
     8221 gttacccgcg aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg
     8281 agccgttctg aagtgttgcg tagcttcctg gacgattaat cggtaggccg gatcaggcgt
     8341 tacgccgcac ccggcactag tctctctgca caaacgccac cttt
//
LOCUS       DAJVRP010000139         8364 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVRP010000139 DAJVRP010000000
VERSION     DAJVRP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.139"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            24..353
                     /locus_tag="OQN12_003631"
     CDS             24..353
                     /locus_tag="OQN12_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS1655814.1"
                     /translation="MGYSRLDDRYIEDDIFRALFHQEMIKRVSEYHSDNFQYTIKIDE
                     VYRSDLAAYRAYGNADLRWVFRVLVGHESEMEEMPAGTTLTLPDVAWLRNKIRDYASA
                     EPEIENA"
     gene            346..1542
                     /locus_tag="OQN12_003632"
     CDS             346..1542
                     /locus_tag="OQN12_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1655815.1"
                     /translation="MPDFLKKQDGRYITDGLSSKDFTRLFEIIRKEQTRKRRQAHRTL
                     TPGRLRNKSAEDILKLGKKKGGTFFTRDDLKGFEKLRSKTREKYDSKTAGITYAQLVA
                     SSQAIDIKRANNAVDDGSGIKRATPVSLRHNVINIRVEASDISVHQHHIVRIRFEEWD
                     QMVDDIAEDDKSALKITKSLCAGRVSFDCDCGRHQYWYRYIATAGNFALAPPKEYAYP
                     KVRNPKLQGVACKHVIHSMTRLQSASWQMSIARALQKAATQIAFGDDRRRTTKHFSKE
                     DEKEFNRNRSSKTNVEAAKREWRLYQKRQAALSTKLAKDNGKIDKLRDQLTRARKLSD
                     AQKKRAAAKEAALQREKQKNKELQQRLADQFALKKQAFIDALVMAGTSPEQAEKMFME
                     YVKKGS"
     gene            complement(1577..1936)
                     /locus_tag="OQN12_003633"
     CDS             complement(1577..1936)
                     /locus_tag="OQN12_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1655816.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEMNTPEGDFFEIMCLLI
                     EEYEKKHYPIEPPSPIEAIKFRMEQQGLTVKDLEPAIGKSNRVYEVLNGTRNLTLPMI
                     RRLHSQFGIPLESLVGV"
     gene            complement(1940..2242)
                     /locus_tag="OQN12_003634"
     CDS             complement(1940..2242)
                     /locus_tag="OQN12_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCS1655817.1"
                     /translation="MKIISIKTLRDFWAKNPDAEQPLKAWVDEATKADWKNPAEIKEQ
                     YRTASILKNRRVVFNIKGNDYRLIVAIAYQRGWMFVKFIGTHKEYDAIEADTIELE"
     gene            2388..3179
                     /locus_tag="OQN12_003635"
     CDS             2388..3179
                     /locus_tag="OQN12_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1655818.1"
                     /translation="MGRFDEWFADDANLASKESEHDSTQRETPATVEADAGSAPEHGN
                     AGPDSFSILEEQSTAEADSHDNFADSNGGLTPDAGESDIAILPPSLAGREPTPQLKAR
                     YNGHKAFNDQIRADWMLIIESSPDAFQALLYRPDVGTYGVVSDETGEESFTELDNNQR
                     ELTYQEPEIVYVLDNPDGRDSFHAIDADGEQDGLTDDVLILRIAANNVPVGSILEWNE
                     EMVNGVARRWWYVHRIFSYGTQHVGSLYYCIPARNFDTTQNGVIE"
     gene            3176..3943
                     /locus_tag="OQN12_003636"
     CDS             3176..3943
                     /locus_tag="OQN12_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HCS1655819.1"
                     /translation="MTSNKTLLARTGEWQTLRTGKFQTTGFENVDNAFATLISNIFSD
                     ILLVAPAPEEKRFASFLNRPPAERVYMARFDNAIEFLKAVRRANAGQGRKPENQNINR
                     DALPLVNISRTMDINYINDDQQIDRKKVASFCEPDTGTPLAELEYTQAILTYDVTLMA
                     TDKATMSLMCNSLGARLRLMTGTQFEATTHLVRVPVPLICSIQDAKEVGFTDVSAPIG
                     EERIYATQAPISVIADVITAWELDAKRIITETSISMG"
     gene            3947..4927
                     /locus_tag="OQN12_003637"
     CDS             3947..4927
                     /locus_tag="OQN12_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail length tape measure protein"
                     /protein_id="HCS1655820.1"
                     /translation="MAQELQQYFLQSVLINDNKVPRDWIFTAVYVEKTSLKAPLLKLE
                     IHDATGTVIDDWKAKYGASLVAEMGDPNGNAGTFKTDFFVTSAMLAGDVVTVIAVSED
                     VRRFKIPSPRTNLHTNKTPDAIFKAYSGKLKITSSVLKRAVTYHLNAGDKPSKMLSEI
                     ARDKGALCWVCRGEFNFYTLADLMKQTPSFTYEGNNPKAEYTLSKMRLLQQEHATTAK
                     NQYRFVGYSMTDGYVEYGDSSLPVRYISDSDMETLRNMQLSLVPKMDIEVAGNPDIKP
                     GMVIEIIVYRYDQENRIDESMPRKLIVKNVAHFEDRVGYTTRMILGVPNK"
     gene            4924..5577
                     /locus_tag="OQN12_003638"
     CDS             4924..5577
                     /locus_tag="OQN12_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS1655821.1"
                     /translation="MKRRAQIVGTVHPAGLMRAQVRVLPDWNGVPDDDLPWAEYLLPI
                     GNAFVPTVKGDLVWVEFPYLDVNGRIDTRRPMIVGAAQDAPGGVPNVAPEASGNGSGW
                     TPPEVDGAPPRPQISATKDFVIHRNNILEVRTAGGGYEIANTAAGSRIGMNESGQIYI
                     IGPADVIVNAGGSVNVKSANNINVNGENIAVTANGDIAFKAGGTFQATAGNFDFKKG"
     gene            5637..6542
                     /gene="rdgC"
                     /locus_tag="OQN12_003639"
     CDS             5637..6542
                     /gene="rdgC"
                     /locus_tag="OQN12_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009444180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1655822.1"
                     /translation="MRSVAFKNIFIYRLSREVNWDTAEVNAALSKFVFTPCGSQDMAK
                     AGWTPILGDNLTHEYQGFLLMQHKREEKILPSQVLKEELQKKILKLEEEQDRKLKKTE
                     KDSLKDEVLHSLLPRAFTRKSLAKILIDRSNHLVFVEASSAKKAEDQLALLRKSLGSL
                     PVIPFTPREPLEITMTEWFKNSFPAGFTAGEDATLKGLLDNGGAVRCNKVDLQSDEIM
                     SHIEAGKVATTVAINWMDRASFRVNDDMSIKALTFCDDLYDQNDDIDREDVAQRFDAD
                     FVLFTGELSALFNALVEAIGGEAER"
     gene            6526..7206
                     /locus_tag="OQN12_003640"
     CDS             6526..7206
                     /locus_tag="OQN12_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655823.1"
                     /translation="MKQNDNNRPLVVSLCDFTGAMVAPWLEYGVDAAIVDPQHLSTSD
                     ERMQSGAVLTRISAIIDSDEVYAFLRKNLQRIVFLAGFPPCTDLAVSGARWFSDKANK
                     DPVFQFKAMQVVWQCYDIAKMIGCPYMIENPVSKISTFWRKPDHIFHPYFFTAYCQED
                     NYTKKTCLWSGQGFVMPDALMDESLGKPDNRIHAAPPGPDRANFRSATPHGFAKAVFE
                     ANKGVLYE"
     gene            7199..8104
                     /locus_tag="OQN12_003641"
     CDS             7199..8104
                     /locus_tag="OQN12_003641"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000045489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCS1655824.1"
                     /translation="MSKSIASIAIEKQDTMAEISYMRTIRTPDEYERPIFKWVGGKFS
                     ELPTVLEHLPHGKRLIEPFVGGGSVFTNAGFRHNLLNDINGDLINFYQTLQREGHSLV
                     TLSYSFFQNYNNADGYLEVREAFNRGKYDQLHHAAAFLYLNRHCFNGVTRYNQNGEFN
                     VGYGKYKAPYFPHAEMEAFLADDVLKNTSFVSGDFAGVIEAAGEGDVIFCDPPYEPLP
                     DTEGFTSYSGNSFRFDEQKRLVSLLVEAHQRGAKVVITNSGAPSIRELYEGNGFKVHH
                     MAARRSVSCKASTRVVANDIIAIMK"
     gene            complement(8153..>8364)
                     /locus_tag="OQN12_003642"
     CDS             complement(8153..>8364)
                     /locus_tag="OQN12_003642"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1655825.1"
                     /translation="ADDFLSVINPESLVIKQGFAEPSLKDAVAGKAFQFEREGYFCLD
                     SRHSTAEKPVFNRTVGLRDTWAKLG"
BASE COUNT         2347 a         1959 c         2035 g         2023 t
ORIGIN      
        1 ggttatgact gagaggcttt gttatgggct atagcagact cgacgatagg tacattgaag
       61 acgatatttt tcgtgcgctg tttcaccagg aaatgattaa gcgggtatcg gagtatcact
      121 ctgataattt ccagtacacg ataaagattg atgaagtata tcgttcagac cttgcagcct
      181 acagggcgta tggcaatgca gatttgcgct gggtattccg ggtgctggtg ggccatgagt
      241 cagaaatgga agaaatgccc gccgggacca cgttaactct tcctgatgtg gcatggctga
      301 ggaacaagat ccgtgattac gcgagcgcgg aaccggagat agaaaatgcc tgatttcctg
      361 aaaaaacagg acgggcgcta tatcactgac ggcctgtcct ctaaggactt cacgcgttta
      421 ttcgagatta tcaggaaaga acaaacccgt aagcgccgac aagctcaccg gacgctgacg
      481 ccaggtagac tgaggaacaa atccgccgaa gatattctca agttagggaa gaaaaaaggc
      541 ggcacgttct tcacgcgaga cgacctgaaa ggtttcgaaa agctacggag taaaacgcgc
      601 gaaaaatatg acagcaagac ggctggcatc acatacgccc agctggtggc atccagccag
      661 gcaatcgata ttaagcgtgc aaataacgcc gtggatgacg gttctggtat caaaagagct
      721 acccccgtat ctcttcgcca caatgtgatt aatatccgcg tagaagcatc ggatatatcc
      781 gtccaccagc accatatcgt ccggatacgc tttgaagaat gggatcagat ggttgatgac
      841 atcgcagaag acgataaatc agctctcaaa atcactaaat cactgtgcgc cgggcgagtg
      901 tctttcgatt gtgactgtgg tcgtcatcaa tactggtatc gttacatcgc cactgcgggt
      961 aactttgccc tggcaccgcc aaaagaatac gcctatccaa aagttcgcaa cccgaagctg
     1021 caaggcgtcg cctgtaaaca cgtgatccac tcaatgacgc ggttacagtc cgccagttgg
     1081 caaatgagta ttgctcgtgc gctacaaaag gctgcaacgc aaattgcatt tggtgacgat
     1141 cgccgccgta caactaaaca cttctcaaaa gaagacgaga aggagtttaa tcgcaatcgt
     1201 agcagtaaaa cgaacgttga agccgccaaa cgcgaatgga ggctctatca gaagcgccag
     1261 gccgctttaa gtacaaaact ggcaaaggac aacgggaaga ttgacaagct acgtgaccag
     1321 ctgaccaggg ccagaaagtt gtcagacgca cagaaaaaac gggcggctgc aaaagaagcg
     1381 gccttgcaac gtgagaaaca gaaaaataag gaacttcagc aacgccttgc cgatcagttc
     1441 gcactgaaga agcaggcgtt cattgatgca ctggtcatgg caggaacgtc acctgagcaa
     1501 gctgaaaaaa tgtttatgga gtacgtgaag aaaggttctt gattgacaac aaaggcggtc
     1561 atacagaccg ccaaactcat acaccaacca ggctttcaag aggaatgcca aactgggaat
     1621 gtaaacgacg aatcataggt aacgttaaat ttcgtgtccc atttagcact tcgtaaacac
     1681 gatttgactt accaatcgcc ggttccagat ccttcacggt cagcccttgc tgttccatac
     1741 ggaatttaat ggcttcaata ggtgatggtg gctcaattgg gtaatgcttt ttctcgtact
     1801 cttcaatgag caggcacatt atttcaaaga aatcgccttc cggtgtgttc atttccggtt
     1861 cgttatcgaa catcggttcg accgcacgca gtgcggcctc ataatcttgt tctgtacgga
     1921 taggtttaat gttcatgatt tactccagtt ctattgtatc cgcttcaatg gcatcgtatt
     1981 ccttatgtgt tccaataaac ttaacgaaca tccatcctcg ctgataagca attgcgacaa
     2041 ttaacctgta atcatttccc ttaatattga acaccacgcg cctgttttta aggatgctgg
     2101 ctgtccggta ctgttctttg atttcagccg gatttttcca gtcagccttt gttgcttcat
     2161 caacccatgc ctttaatggc tgttctgcat cgggattctt cgcccagaaa tcccttagtg
     2221 tttttatcga aattattttc atcacagatt ccttttctct gtaagcagag tagtcccaat
     2281 atgggacaat gtaaagcact ttagtcccat gctgggatat gaattgaaat cacaatctcc
     2341 agataatgca ccctcgatac ctgataaatc gacctttgag gatgcttatg ggccgttttg
     2401 acgaatggtt tgctgacgat gcaaacctgg caagtaagga gtcagaacat gacagcacgc
     2461 agagagaaac gccagcgacg gttgaagcgg atgcaggaag cgcgccggaa catggcaatg
     2521 ccggaccgga ttcattcagc attctggaag agcaatccac cgccgaagct gatagccatg
     2581 ataattttgc tgatagcaac ggtggcctta ctccggacgc tggcgaatca gatatagcca
     2641 tacttccacc ttccctggct ggcagagaac cgactccaca gttaaaagca cgctataacg
     2701 ggcataaagc ctttaatgac caaatccgcg cagactggat gctgattatt gaatccagcc
     2761 cagacgcgtt tcaggctctc ttatatcgac cagatgttgg cacatatggg gtagtcagcg
     2821 acgaaacagg agaagagtca ttcactgaac tggataacaa ccaacgcgaa ctgacttacc
     2881 aggaacctga aatcgtttat gtgctggata accctgacgg gcgtgactct ttccatgcaa
     2941 ttgacgcaga cggtgagcag gatggcttaa ccgatgatgt tctgattctg cgtattgcag
     3001 caaataacgt ccccgtcggc tcaattcttg aatggaatga agagatggta aacggcgtag
     3061 cccgccgctg gtggtacgtg caccgtattt ttagctacgg cacacagcat gttggttcgc
     3121 tttactactg catacctgcc aggaattttg atacgactca aaacggggtg atcgaatgac
     3181 ttcaaataaa accctcctgg cgcgaaccgg tgaatggcaa accttacgca caggaaaatt
     3241 ccagaccacg ggctttgaaa acgtggataa cgcgtttgcg acgctaatca gcaacatttt
     3301 ttctgatatc ttactggtgg cccccgcgcc ggaagaaaaa cgctttgcgt cattcctgaa
     3361 tcgtccacca gcagagcgtg tctatatggc ccgtttcgac aatgcgatcg agtttcttaa
     3421 agcagttcgt cgcgcaaatg ccgggcaagg gagaaaacct gaaaaccaga acattaaccg
     3481 ggatgctctc cccctggtca atatctcacg cactatggat atcaattata tcaacgatga
     3541 tcagcagatt gaccgcaaaa aagtagccag tttttgcgaa ccagataccg gaacgccttt
     3601 agcagaactg gaatacaccc aggccattct gacgtatgac gttacgttaa tggcaactga
     3661 taaagcgacc atgagtctga tgtgtaattc gctgggcgca cggcttcgcc tgatgacagg
     3721 tacacaattt gaagcaacca ctcaccttgt tcgtgtcccg gtcccgctga tttgctcgat
     3781 acaggatgcc aaagaagtcg gctttacgga tgtttcagca ccaattggag aagagcgtat
     3841 ttatgccaca caagcgccga taagtgtgat tgcagacgtg atcacagcat gggagttgga
     3901 cgcaaaacgt attattaccg aaacctcgat atctatgggg tgataagtgg cccaggagtt
     3961 acaacaatat tttctacagt cagtgctcat taacgataac aaagtgccac gagactggat
     4021 ttttaccgca gtatatgtag aaaaaaccag cctcaaagcc cctttgctaa aactggaaat
     4081 tcatgacgct accggcaccg taattgatga ctggaaagcc aaatacggtg catcgctggt
     4141 ggctgaaatg ggcgatccaa acggtaatgc tggcactttt aaaacagatt tctttgttac
     4201 gtctgcaatg ctggctggtg atgttgttac cgttattgct gtcagtgaag acgtgcgcag
     4261 gtttaagatc ccctccccgc gcacaaattt acataccaac aaaacaccag acgctatatt
     4321 caaggcatat tccggcaagc ttaaaattac cagcagcgtg ctaaaacgcg cagttacata
     4381 tcatctgaat gccggtgaca aaccgtcaaa aatgctttcg gagatagcgc gagacaaagg
     4441 tgcattgtgt tgggtatgtc ggggggaatt taacttttac accctggctg atctgatgaa
     4501 gcaaacgcca tcatttacct acgaggggaa taaccctaaa gcagaataca ctttgtccaa
     4561 aatgcgcctg ctccaacagg aacatgcgac aacagcaaag aatcaatatc gttttgttgg
     4621 gtattccatg accgatggct acgtcgaata tggcgatagc tcactcccag tgcgttatat
     4681 atccgactct gatatggaaa ctcttcgcaa tatgcagctg tctctcgtcc ccaaaatgga
     4741 tatagaagtc gcaggcaatc ctgatataaa accggggatg gtaatagaga ttatcgtata
     4801 ccgctatgac caggaaaacc gcattgatga atcaatgccc cgtaagctga tagtaaaaaa
     4861 tgttgcgcac tttgaggacc gcgtaggcta cacaacacga atgatattgg gagtgccgaa
     4921 caaatgaagc gtagagcgca aattgttgga actgtgcacc cggcagggct tatgcgtgct
     4981 caagtccgtg ttttacctga ctggaatggc gttcctgatg acgatctacc ctgggcggaa
     5041 tacctgttac ccatcgggaa cgcttttgta cctacagtca aaggtgacct tgtctgggta
     5101 gagtttcctt atttagatgt taacggtcga atagacacca gacgcccaat gatagttggc
     5161 gcggctcagg atgcgcctgg cggagttcca aacgtcgcgc cggaggcatc aggcaatggt
     5221 agcggctgga cgccgccgga agtagatgga gcacctcccc gcccccaaat ctcagcaacg
     5281 aaagacttcg ttattcaccg caacaatatt cttgaagtac gaactgctgg tggcggctat
     5341 gaaattgcca atacggcagc aggctcaagg attggcatga atgagtccgg gcagatatat
     5401 atcattggtc cggctgatgt aatcgtgaat gcaggaggga gtgttaacgt caaatcagcc
     5461 aacaacatca atgttaatgg ggaaaatatt gctgtcaccg ctaacgggga catagctttc
     5521 aaggcagggg gaacctttca ggccactgct ggcaacttcg atttcaagaa aggatagaaa
     5581 atgcacccat ttgtgttaga taattaacgc atattttcta acatttatgg tgagaaatga
     5641 gatcagtcgc tttcaaaaat atcttcatct atcgtctttc acgcgaagtt aactgggata
     5701 ccgcagaagt aaatgcagcc cttagcaagt ttgtgtttac cccatgcggt tctcaggata
     5761 tggctaaagc tggttggacc ccaattcttg gcgacaacct cacccatgaa taccagggtt
     5821 ttcttctgat gcagcataaa cgagaggaaa aaatcctgcc ttctcaagta ctcaaagaag
     5881 agttacagaa aaaaatcctg aaacttgaag aagagcaaga cagaaagctg aagaagaccg
     5941 aaaaagattc gctgaaggat gaagtgcttc actctcttct tcctcgtgct ttcacgagaa
     6001 aatcactcgc aaaaatcctg atcgaccgca gcaaccatct ggtattcgtt gaggcaagca
     6061 gcgccaaaaa ggcagaggac cagctggctt tattgcgaaa atcgctcggc agcctgcctg
     6121 tcattccatt cacaccacgc gaaccgctgg aaattacaat gaccgagtgg tttaaaaaca
     6181 gcttccctgc cggatttaca gcaggtgaag acgccacatt aaagggtctg ctcgataatg
     6241 gtggtgccgt tcgctgcaac aaagttgatc tacagtccga cgagattatg tctcacattg
     6301 aggcggggaa agtagctacg accgttgcaa ttaactggat ggaccgcgcg tcgttcagag
     6361 tcaacgatga tatgagtatc aaagccctga cattctgtga tgatctctat gaccagaacg
     6421 acgatatcga ccgggaggat gtagcacaac gatttgatgc tgatttcgtg ctctttacgg
     6481 gcgaattatc tgcactgttt aacgcgctgg tggaggctat tggcggtgaa gcagaacgat
     6541 aataaccgac cgcttgttgt gagtctgtgt gattttacgg gggcaatggt tgccccctgg
     6601 ctggaatacg gtgtcgatgc cgctatcgtc gatccacagc acttatcgac cagtgacgag
     6661 cggatgcaat caggtgccgt tctgacgcgt attagtgcga tcatcgatag cgatgaagta
     6721 tacgcttttc tccgtaagaa tttgcagcgc atcgtgtttc tggccgggtt cccaccgtgt
     6781 actgacctgg ctgtttccgg cgcgcgctgg ttttcggaca aggctaataa agatccggtt
     6841 ttccagttca aagcaatgca ggttgtctgg cagtgttatg acattgcaaa gatgatcggt
     6901 tgtccgtaca tgattgaaaa tccagtcagc aaaatatcga cattctggcg taagccagat
     6961 cacatctttc acccttactt cttcactgca tattgtcagg aagataacta caccaaaaaa
     7021 acatgcctct ggagcggcca gggctttgtc atgcctgatg ccctaatgga cgaatcatta
     7081 ggcaagccag ataacagaat ccatgccgcg ccaccagggc ctgacagggc taattttcga
     7141 agtgctactc cacatggatt cgcaaaggca gttttcgaag cgaataaggg ggtgctctat
     7201 gagtaaatct atagcaagca tagccataga aaaacaggat acgatggctg aaattagcta
     7261 tatgcgcacc attcggactc cggatgaata tgagcgtccg atcttcaaat gggtaggcgg
     7321 taaattctca gagttgccta cagtgcttga gcatctacca cacggcaagc ggttaataga
     7381 accgttcgtt ggtggcggtt ctgtatttac gaatgcagga tttcgccaca acctgcttaa
     7441 tgatattaac ggtgacctga ttaacttcta tcagacattg cagcgagaag gacattcgct
     7501 cgtcacgctg tcatatagtt ttttccaaaa ttacaacaac gctgacggtt acctcgaagt
     7561 gcgtgaggca tttaacagag gaaagtatga ccagctacat catgctgccg cctttttgta
     7621 ccttaatcgg cactgtttta atggcgtaac gcggtacaac caaaatggcg agttcaacgt
     7681 ggggtatggc aaatacaaag cgccctactt cccacatgca gagatggagg catttttggc
     7741 tgatgacgta ctcaaaaaca cgtcctttgt atcaggtgac tttgctggcg tcatcgaggc
     7801 ggctggtgaa ggcgatgtga ttttttgcga tccgccgtat gaaccgctgc cggatacaga
     7861 agggtttacc agttattcag gaaatagctt ccgtttcgac gaacagaaac ggctggtatc
     7921 tctattggtg gaagcccacc agcgcggcgc taaggtagtg attacaaata gtggtgcgcc
     7981 aagcatccgt gaactatatg aaggaaacgg gtttaaagta catcatatgg cagccagacg
     8041 gtcggtttcc tgcaaggcgt caacacgtgt agttgctaac gatataattg caataatgaa
     8101 gtaaaaaacg ccgcagtagc ggcgtttact tttttatgtg gcgaaatacc tattaaccta
     8161 acttcgccca ggtatcacgc agcccaacgg tgcggttaaa taccggtttt tccgccgtgg
     8221 aatggcggct gtcgaggcag aaataacctt cacgctcaaa ctggaatgct ttacccgcaa
     8281 ccgcatcttt cagcgacggt tcagcaaagc cctgtttgat caccagcgat tccgggttaa
     8341 tcaccgacag gaaatcatcc gcag
//
LOCUS       DAJVRP010000140         8260 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVRP010000140 DAJVRP010000000
VERSION     DAJVRP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.140"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            68..316
                     /locus_tag="OQN12_003643"
     CDS             68..316
                     /locus_tag="OQN12_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCS1655826.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            381..749
                     /locus_tag="OQN12_003644"
     CDS             381..749
                     /locus_tag="OQN12_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1655827.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            843..1496
                     /gene="nfsB"
                     /locus_tag="OQN12_003645"
     CDS             843..1496
                     /gene="nfsB"
                     /locus_tag="OQN12_003645"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS1655828.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1604..2851
                     /gene="ybdG"
                     /locus_tag="OQN12_003646"
     CDS             1604..2851
                     /gene="ybdG"
                     /locus_tag="OQN12_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1655829.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="OQN12_003647"
     CDS             complement(2919..4295)
                     /gene="pheP"
                     /locus_tag="OQN12_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1655830.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="OQN12_003648"
     CDS             complement(4396..7539)
                     /gene="cusA"
                     /locus_tag="OQN12_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCS1655831.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="OQN12_003649"
                     /pseudo
     CDS             complement(7551..8069)
                     /gene="cusB"
                     /locus_tag="OQN12_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2015 a         2306 c         2012 g         1927 t
ORIGIN      
        1 aagccgggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
       61 caatgttatg aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc
      121 tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact
      181 ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt tttgtctgtg
      241 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
      301 ctcgctggcg gattaaacgt cgattatcgc cgaacttcgc ggcatacttt gcttattctc
      361 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctag
      421 ctcttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
      481 aaattggcgg caagattttt atgctgtcgt cggagctacg cggcgtcccc tttatcaatc
      541 tgaagtccga tccacaaaaa tccctgttaa atcagcaaat atacccaagc attaagccag
      601 ggtatcacat gaataaaaag cactggattt cggtgtattc cggcgaggaa atctccgaag
      661 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
      721 gcgatcaaaa aagagtgcgt ccaggctaaa gcgggaatct atagcgcatt tttctcgctt
      781 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attacttaca catggagtct
      841 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagca
      901 aaaaacttac cccggaacag gccgagcaga tcaaaacgct actgcaatac agcccatcca
      961 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
     1021 ttgccaaatc cgctgccggt aattatgtgt tcaacaaacg taaaatactt gatgcctcgc
     1081 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
     1141 accaggaaga tgccgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
     1201 gtcgcaagtt cttcgccgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
     1261 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg
     1321 acgcggtgcc catcgaaggt tttgacgccg caatcctcga tgcagaattt ggtctgaaag
     1381 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
     1441 acgctacgct gccgaaatct cgtctgccgc aaaacaccac cttaaccgaa gtgtaattct
     1501 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
     1561 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
     1621 ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
     1681 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
     1741 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
     1801 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
     1861 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
     1921 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
     1981 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
     2041 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
     2101 gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc
     2161 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
     2221 ctggctggaa atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac
     2281 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
     2341 tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag
     2401 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
     2461 taaagcgcat ttgttaaagc cttatttaac cagccgccat caggaaatta atgagtggaa
     2521 tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc cgaatgacca atattggaac
     2581 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
     2641 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
     2701 tactaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
     2761 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat
     2821 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
     2881 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
     2941 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
     3001 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg cgatgcaaag atagttgccg
     3061 aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat cgccgcgcga
     3121 aaacgcagat gcgccaggca gatcataatc cagttcaaca gcaacgttgc taccaccagc
     3181 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa
     3241 gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct gacgcgagtc
     3301 aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
     3361 accccgctgt tatacaccga cagcgatgcc actagaatga cgaagttcag cgcagaagct
     3421 accacattgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
     3481 tccacccacg gatagagtgc cagtaaaacc accagtgaac cgatataaaa cagcaggatg
     3541 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
     3601 gctgtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
     3661 atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg
     3721 gctttctcgc cgccgtaacc agaaaacagc agccacaggc caaagccgat cataccgatg
     3781 atcgccagca ctttaatcag cgcaaaccag aactcggttt cgccatataa gcgcacgttc
     3841 accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
     3901 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
     3961 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
     4021 taggcaaagt gggcaaatga accggatacc ggctcttcga ccaccatctc gccaagctgg
     4081 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
     4141 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
     4201 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac
     4261 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
     4321 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
     4381 acgaggtatc ctgctttaat tccgtacccg atgtcggtgc agccacatca gcttatacgc
     4441 cgccgggata ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc
     4501 ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc
     4561 ggcgataatc accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata
     4621 caacgcctca tccagcttct gctcgctgaa tgtttgtgga ttattcaacg acggctcggc
     4681 ctctatggcg tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc
     4741 aagggcaata aagccggtac ccgtcgccac agaaagatga aagcccatcc accacagtaa
     4801 ccagatgccg cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac
     4861 ccgacggaat gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag
     4921 tttcagctta tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt
     4981 gcccggtttt aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac
     5041 catgtcacga tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt
     5101 cagcatcgac ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg
     5161 cttcatcggc gtcaggatcg gcaactggcg cagtgcctgc ggactatcgc gccagctttg
     5221 cggataacgc agattaattg gataacgggc aatcccttcc accgtttcgc caaccatcgc
     5281 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgtgc
     5341 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagcc gctcagcaag
     5401 cgcagaagct acgcctggca ccgttcgcgc cacttcttca atttgctcag ccatcgcgtc
     5461 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
     5521 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagacg
     5581 cacggtgtta tccagttcct caatgatttt gtccattgtc atgcctggcc gccactgatc
     5641 ctgtggctta agctggatgg tcgtttctac catctccagc ggagcagaat cggtggcggt
     5701 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
     5761 gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
     5821 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
     5881 cggccagaga accgtcagca ccgaaagcgc cgccaccagc agcgtggttt tcggccagtg
     5941 cagtactttc agcaacagcg gatgataaac acgaatcaaa aagcgattga gcggattact
     6001 gctttccggc ggaattttgc cacggatcca gtaacccatc aggataggga tcactacgat
     6061 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccaacg ggccaaacag
     6121 acgcccttcc tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag
     6181 actgataaac agcgccggcc ccacttcaac agacgcatcg gtgatcacct gccagcgcgt
     6241 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
     6301 attctcgatc atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccacc
     6361 cagcgacata atattggcat tcagcccctg gaagggcatg acaataaaag caatacacaa
     6421 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
     6481 gcagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
     6541 ccggtcaatg agctggctgc ggtcgtatgt tgtaactatc tccacgcctt ccggcagact
     6601 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttgcc
     6661 ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgtttagtt cggcaatgcc
     6721 ccggcgcatc ttcgggccaa tctggacctt cgcaacatcg cgcagataaa cgggcacgcc
     6781 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgta gatagccgct
     6841 ggcgcgcacc atatattctg cttccgccag ttcgattgac gaaccgcccg cttcctggtt
     6901 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
     6961 aggatcgata accacctgat actctttcac cacaccgcct accgacgcca cttccgcaac
     7021 gtcaggaatg gttttcagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc
     7081 aagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
     7141 ggcatctggt cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
     7201 gtactccagc acccgcgaac gcgcccagta cggatcggtg ccatcttcga aaatgacata
     7261 cacataagag tcgccaaact gcgagaaacc acgcacagtc ttcgcgccag gcaccgacaa
     7321 catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg cctggccggg
     7381 atagctggtt ttaataatca cctgcacatc ggagagatcc ggcaacgcat ccactggcgt
     7441 attaatgatg gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa
     7501 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
     7561 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
     7621 atcaggaaca ggccgccgga aaccaccttt tcaccttccg ccagaccaga gcgtaatgcg
     7681 gtgacgcctt gcgatgcctg gaaaacagca acgcgtttcg gtacaaagcg cccgtcggca
     7741 tcaacggtaa tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc
     7801 agcatcggtt cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc
     7861 tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca
     7921 ggtagcagcg tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagg
     7981 gtaaactgcg aggcatcttt caccagccag gcgatggact ccgggatcgc agcagtgacc
     8041 cacaccgggt ccataccctg aattcgcgat tacgcgcaat ccccctgaga accgccatga
     8101 acaagggttt catgatttgt gattttagtt tggtacgcaa tttggtacac aacactattt
     8161 tcacttcagc gggtagtcat caaactcacc ataacgagca tcgttgatga tgtatgtgat
     8221 caccacaaat atcacatctg gactagttcc gtcttcatgc
//
LOCUS       DAJVRP010000141         7916 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVRP010000141 DAJVRP010000000
VERSION     DAJVRP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.141"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            237..740
                     /gene="dps"
                     /locus_tag="OQN12_003650"
                     /gene_synonym="pexB"
     CDS             237..740
                     /gene="dps"
                     /locus_tag="OQN12_003650"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1655832.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1144..1890
                     /gene="glnH"
                     /locus_tag="OQN12_003651"
     CDS             1144..1890
                     /gene="glnH"
                     /locus_tag="OQN12_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1655833.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2029..2688
                     /gene="glnP"
                     /locus_tag="OQN12_003652"
     CDS             2029..2688
                     /gene="glnP"
                     /locus_tag="OQN12_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1655834.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2685..3407
                     /gene="glnQ"
                     /locus_tag="OQN12_003653"
     CDS             2685..3407
                     /gene="glnQ"
                     /locus_tag="OQN12_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1655835.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3668..5892
                     /gene="ybiO"
                     /locus_tag="OQN12_003654"
                     /pseudo
     CDS             3668..5892
                     /gene="ybiO"
                     /locus_tag="OQN12_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="OQN12_003655"
     CDS             complement(5889..6815)
                     /gene="rlmF"
                     /locus_tag="OQN12_003655"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1655836.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7091..7351
                     /locus_tag="OQN12_003656"
     CDS             7091..7351
                     /locus_tag="OQN12_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1655837.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7616..>7916
                     /locus_tag="OQN12_003657"
     CDS             7616..>7916
                     /locus_tag="OQN12_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1655838.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1934 a         2025 c         1978 g         1979 t
ORIGIN      
        1 cgtatttata tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga
       61 taaataagaa ttgttcttat caatatatct aactcattga atctttatta gttttgtttt
      121 tcacgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg
      181 gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga
      241 gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct
      301 ccgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg
      361 atctttcttt gattaccaaa caagcgcact ggaacatgcg cggcgctaac ttcattgccg
      421 tacatgaaat gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag
      481 aacgtgccgt gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa
      541 ccccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg
      601 ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg
      661 acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta
      721 tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa
      781 gcggcttcct gacaggaggc cgttttgttt tgcagcccac ctcaacgcac ttatttagtg
      841 catccatctg ctatccccag ctgattaagt aaattttttg tatccacatc atcacacaat
      901 cgttacataa agattgtttt ttcatcaggt tttacgctaa ataatcactg tgttgagtgc
      961 acaattttag cgcaccagat tggtgcccca gaatggtgca tcttcagggt attgccctat
     1021 aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa tgagttgcaa aattggcacg
     1081 attttttcat atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat
     1141 gctatgaagt ctgtattaaa agtttcactg gctgcactga ccctggcttt tgcggtttct
     1201 tctcatgccg cggataaaaa attagttgtc gcgacggata ccgccttcgt tccgtttgaa
     1261 tttaaacagg gcgataaata tgtgggcttt gacgttgatc tgtgggctgc catcgctaaa
     1321 gagctgaagc tggattacga actgaagccg atggatttca gtgggatcat tccggcactg
     1381 caaaccaaaa acgtcgatct ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa
     1441 gcgatcgatt tctctgacgg ctactacaaa agcggcctgt tagtgatggt gaaagctaac
     1501 aataacgatg tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt gaagagcggt
     1561 actggctccg ttgattacgc gaaagcaaac atcaaaacta aagatctgcg tcagttcccg
     1621 aacatcgata acgcctatat ggaactgggc accaaccgcg cagacgccgt tctgcacgat
     1681 acgccaaaca ttctgtactt catcaaaacc gccggtaacg gtcagttcaa agcggtaggt
     1741 gactctctgg aagcgcagca atacggtatt gcgttcccga aaggtagcga cgagctgcgt
     1801 gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac
     1861 aaaaaatggt tcagtactga accgaaataa taacgctaca cctgtaaagc gcactggcag
     1921 ttccctctcc cccctgggga gaggattagg gtgaggggcg caaacccgct ccggggccat
     1981 taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat gcagtttgac
     2041 tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat gaccctgtgg
     2101 atttcggtcc tcggtctggc tggcggtctg gtaatcggat tgctggcagg ttttgcacgc
     2161 accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaagt gatccgcggc
     2221 acacctatcg tcgtccaggt gatgtttatc tatttcgccc tgccgatggc gtttaacgac
     2281 ttacgcatcg acccatttac tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac
     2341 attgcggaaa tcacgcgtgg tgcggtgctg tctatccaca aaggttttcg tgaagcagga
     2401 ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc gctggcactg
     2461 cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gcattaaaga cacctcgctg
     2521 tttattgtta tcggcgtggc ggaactgacc cgtcaggggc aagaaattat tgccggtaac
     2581 ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat taccctggtg
     2641 ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa
     2701 acgtctccaa gcactttggc ccaacccagg tgctgcacaa tatcgatttg aacattgccc
     2761 agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc ctgctgcgct
     2821 gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc ctgaaggtca
     2881 acgatccgaa agttgacgag cgcctgattc gccaggaagc aggtatggtg ttccagcagt
     2941 tttacctctt cccgcatctg acggcgctgg gaaacgtcat gtttggtccg ctacgcgtgc
     3001 gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc
     3061 tggcagaacg tgcacatcac tacccttccg aactttctgg tggtcaacag cagcgtgtgg
     3121 cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa ccgacttccg
     3181 ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg gctgaagaag
     3241 ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta gcttcgcggc
     3301 tgatctttat cgacaaaggc cggattgcgg aagatggcaa tccgcaggtg ttgatcaaga
     3361 acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaataa gacacattgc
     3421 ctgatgcgct acgcttatca ggcctacagg atatctggca acttattaaa attgcatgaa
     3481 cttgtaggac ggataaggcg ttcacgccgc atccggcaaa aaagcccgca cgttgtcagc
     3541 aacctgctta atatcccttc ctccctttca cccgaaaggg aggcacacca gattcctctc
     3601 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
     3661 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
     3721 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
     3781 ggaaccggat atcgaacaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
     3841 gacacctcgc gtaaagagtt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
     3901 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
     3961 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
     4021 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
     4081 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
     4141 gcactgccgc tatatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
     4201 aactggttgc agcttccggc gatgattatt ggggcgttta ttatcgacct gctgttactg
     4261 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
     4321 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
     4381 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
     4441 gagagcgccc gttactgggg tcgtcgcctg agctggttaa gcagcctgat tggctatggc
     4501 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
     4561 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt ccgaaataaa
     4621 aaagagatta cccagcatat gctcaacttt gcggagcatt cgctggcttt tttcagcctg
     4681 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcatgctg
     4741 tttttctttt cgttgttcga tccgggcaac agtctgaaat ttatgatggg tgcaacggtg
     4801 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
     4861 gcgaaaacca tcaccctctc gccacatact cagcgtaact atccggagct acaaaaacgg
     4921 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggca
     4981 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
     5041 gcggggcaga aaaccgtaga tatcctgatc cgcatcgcac tcattctttt cttctcggcg
     5101 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
     5161 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
     5221 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
     5281 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
     5341 gtaaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa cactggagat
     5401 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
     5461 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
     5521 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
     5581 gctgataaag ccaatcaggc actgaaagat gcggtaacgg aattgatgga aaacgaagaa
     5641 attcgcgggc tgattattgg tgaaccgaac tttgccggga ttgtcggctt aagcaatacc
     5701 gcgtttacac tgcatgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
     5761 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
     5821 acttatcagg tgctgcctgc tccgggcgcg accccggctg aaccgctgcc gccgggggag
     5881 ccaacgcttt aacgttggcg attaacaaaa cggcggcgct gctcgtcgtt cataaaggtc
     5941 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
     6001 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
     6061 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
     6121 aagttgactt caccgccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
     6181 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgccgcagcg
     6241 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttgtggatg
     6301 atgccgttaa aaatcgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
     6361 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
     6421 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
     6481 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
     6541 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
     6601 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
     6661 accgccagcg gattggcaaa gtctacgctt tgctcccctg cgggtgtaag cgtgaggaat
     6721 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
     6781 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
     6841 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
     6901 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattga
     6961 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag catatctctg tcaatttttt
     7021 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
     7081 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
     7141 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
     7201 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
     7261 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
     7321 gatgttaggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
     7381 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca atgtaaataa
     7441 atgtatttct ttttcgcgca atgggtgata gaaaatcgca tcaagtgata atgcttatca
     7501 aaattactat cacttgcacg aacactatca cgggattaac agtggcatcg catccgcaga
     7561 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
     7621 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg ccggtctttg
     7681 tattggtatt acgcctgtgg cacaggcact cgccgccgaa gggcaagcta acgcggatga
     7741 cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat ctgccgatcc
     7801 gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt ctgaacaagt
     7861 gattaaagat cagggcgcaa ccaaccttac cgacgcgctc aaaaacgtcc ccggcg
//
LOCUS       DAJVRP010000142         7910 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVRP010000142 DAJVRP010000000
VERSION     DAJVRP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.142"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(123..953)
                     /locus_tag="OQN12_003658"
     CDS             complement(123..953)
                     /locus_tag="OQN12_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCS1655839.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OQN12_003659"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OQN12_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1655840.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OQN12_003660"
     CDS             2379..2729
                     /locus_tag="OQN12_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCS1655841.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OQN12_003661"
     CDS             2855..3415
                     /locus_tag="OQN12_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1655842.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OQN12_003662"
     CDS             3418..6384
                     /locus_tag="OQN12_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCS1655843.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OQN12_003663"
     CDS             6451..6828
                     /locus_tag="OQN12_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1655844.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OQN12_003664"
     CDS             complement(7029..7688)
                     /locus_tag="OQN12_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCS1655845.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2241 c         2133 g         1783 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatatgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
//
LOCUS       DAJVRP010000143         7838 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVRP010000143 DAJVRP010000000
VERSION     DAJVRP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.143"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            49..1388
                     /gene="yjeM"
                     /locus_tag="OQN12_003665"
                     /pseudo
     CDS             49..1388
                     /gene="yjeM"
                     /locus_tag="OQN12_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="OQN12_003666"
     CDS             complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="OQN12_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1655846.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4872..5840)
                     /gene="psd"
                     /locus_tag="OQN12_003667"
     CDS             complement(4872..5840)
                     /gene="psd"
                     /locus_tag="OQN12_003667"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1655847.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="OQN12_003668"
     CDS             complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="OQN12_003668"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1655848.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7084..7629
                     /gene="orn"
                     /locus_tag="OQN12_003669"
     CDS             7084..7629
                     /gene="orn"
                     /locus_tag="OQN12_003669"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1655849.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1782 a         2038 c         2110 g         1908 t
ORIGIN      
        1 aagcacctta aaatcaccat catacactaa atcagtaagt tggcagcatt acccgcctta
       61 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
      121 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      181 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      241 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      301 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      361 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      421 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      481 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      541 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      601 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      661 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      721 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      781 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      841 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      901 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      961 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
     1021 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1081 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1141 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1201 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1261 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1321 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1381 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1441 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1501 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1561 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1621 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1681 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1741 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1801 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1861 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga gagcgaccag
     1921 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1981 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     2041 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2101 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2161 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2221 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2281 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2341 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2401 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2461 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2521 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2581 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2641 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2701 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2761 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2821 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2881 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2941 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     3001 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3061 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3121 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3181 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3241 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3301 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3361 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3421 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3481 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3541 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3601 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3661 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3721 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3781 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3841 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3901 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3961 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     4021 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4081 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4141 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4201 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4261 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4321 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4381 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4441 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4501 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4561 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4621 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4681 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4741 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4801 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4861 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4921 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4981 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     5041 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5101 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5161 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5221 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5281 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5341 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5401 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5461 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5521 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5581 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5641 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5701 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5761 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5821 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5881 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5941 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     6001 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6061 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6121 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6181 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6241 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6301 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6361 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6421 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6481 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6541 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6601 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6661 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6721 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6781 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6841 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6901 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6961 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     7021 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7081 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7141 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7201 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7261 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7321 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7381 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7441 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7501 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7561 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7621 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7681 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7741 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7801 cagaaatgcg aaaattacga aagcaaaatt aagtagta
//
LOCUS       DAJVRP010000144         7783 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVRP010000144 DAJVRP010000000
VERSION     DAJVRP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.144"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            53..586
                     /gene="ymdB"
                     /locus_tag="OQN12_003670"
     CDS             53..586
                     /gene="ymdB"
                     /locus_tag="OQN12_003670"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1655850.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            528..2009
                     /gene="clsC"
                     /locus_tag="OQN12_003671"
     CDS             528..2009
                     /gene="clsC"
                     /locus_tag="OQN12_003671"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS1655851.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2017..3174)
                     /gene="mdoC"
                     /locus_tag="OQN12_003672"
     CDS             complement(2017..3174)
                     /gene="mdoC"
                     /locus_tag="OQN12_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1655852.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3568..5103
                     /gene="mdoG"
                     /locus_tag="OQN12_003673"
     CDS             3568..5103
                     /gene="mdoG"
                     /locus_tag="OQN12_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1655853.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5096..7639
                     /gene="mdoH"
                     /locus_tag="OQN12_003674"
     CDS             5096..7639
                     /gene="mdoH"
                     /locus_tag="OQN12_003674"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1655854.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1927 a         1864 c         2059 g         1933 t
ORIGIN      
        1 aaccaaaaag cctgctgtac acttaagaaa caagaaggtg taaggaggcg ttatgaaaac
       61 gcgtattcat gttgtgcagg gtgatattac caaactggcc gttgatgtga ttgtgaatgc
      121 ggctaatccg tcattaatgg gaggcggcgg cgtcgatggg gccattcatc gcgcagcggg
      181 tccggccctg ctggatgcct gtttaaaagt caggcaacag cagggcgatt gccctacggg
      241 gcatgccgtt attacgcttg caggcgatct tcccgctaaa gccgtagtac acaccgtcgg
      301 gccagtctgg cgtggtgggg aacaaaacga agaccagctt ttgcaggatg cctatctgaa
      361 tagcctacga ctggtggcgg caaacagcta tacgtcagtg gcttttcctg caatcagtac
      421 tggggtttat agttaccctc gtgcggcagc ggctgaaatc gcagttaaaa ccgtttcaga
      481 atttattacc cgtcacgctt tacccgaaca ggtatacttt gtctgttatg atgaagaaaa
      541 cgcccacctc tacgaaagac tccttaccca acaaggagat gaatgatttg ccccggctgg
      601 cgagcgcggt gctgccactg tgttcgcaac atcccggtca gtgtggtctt ttccctctgg
      661 agaaaagtct ggatgcgttt gccgcccggt atcgtctggc cgaaatggca gagcatacgc
      721 tggatgttca gtattacatc tggcaggacg atatgtcggg gcggttactg ttttccgccc
      781 tgttagccgc agcaaagcgt ggcgttcgcg tccgtttgtt gctggacgac aacaatacgc
      841 ccggacttga cgacattttg cgcttgcttg acagtcatcc acgcattgaa gtccggcttt
      901 ttaatccttt ctcgtttcgc ttgctacgtc cgcttggata tatcaccgac ttttcccgtc
      961 tcaatcgccg gatgcacaat aaaagtttca ctgtcgatgg cgtggttacc ctagtgggag
     1021 gacgaaatat tggtgatgcc tattttggtg caggggaaga gccacttttt tcggatttag
     1081 atgtcatggc aataggcccc gtggtagagg atgttgccga tgatttcgcc cgctactggt
     1141 attgcaaatc ggtttcaccc ttacagcagg tgctggatgt cccggaaggt gaaatggcgg
     1201 atcgcatcga gttacccgcc tcctggcata acgatgccat gacgcatcgt tatttacgca
     1261 aaatggaatc cagtccattt ataaatcatc tggttgatgg aacgttgccg cttatctggg
     1321 cgaagacacg tttattaagt gatgatccgg cgaaagggga gggcaaggca aaacggcatt
     1381 cactgttacc gcagcgcctg ttcgatatca tgggctcacc cagtgaacgc atcgatatta
     1441 tctcttccta ttttgtaccg acacgcgcag gtgtggcgca actcttacgg atggtgagaa
     1501 aaggggtaaa gattgcgatc ctaaccaatt ctcttgccgc taacgatgtt gctgtcgtcc
     1561 atgccggata cgcgcgctgg cgcaaaaaat tgctccgcta tggcgtggaa ttatatgaac
     1621 tcaagccgac gcgtgaacaa agcagtacgt tacacgatcg cggcataacc ggtaattccg
     1681 gtgccagcct gcatgctaaa acttttagca tcgatggtaa aacggtgttt atcggctctt
     1741 tcaatttcga tccgcgttca acattgctca atactgaaat gggcttcgtg atagagagcg
     1801 aaacgctggc acagttaatt gataaacgct ttattcagag ccagtacgat gcggcctggc
     1861 agctccgtct ggacaggtgg ggacggatca actgggttga tcgtcatgca aagaaagagg
     1921 ttattctcaa aaaagaaccc gccaccagtt tctggaagcg ggttatggtc agactggcgt
     1981 cgatattgcc cgtggaatgg ttattgtaaa tgtggcttaa cgggctggtg ctttatcgtt
     2041 ctcacgctta acaaccggtt taccagagaa caaaaacttc agtaacggga tgcgtaaatg
     2101 aatttcatac agaattatcg caatccctac tacgaatatc aggccacaga gaaaaccaag
     2161 ccagttggag gtgatgtgcg gtgtaatgta tgcgccgaaa aacagcgtta acgggtggtg
     2221 aaccagatag ataaacagcg atgcgttaac aaaataagtc acccgcgctg actggaagtt
     2281 aagcaaacgg tggccaaagg agaagaccac attcaccatc cacaggccga ggaccatggt
     2341 gatcaccgac tcggtttcgt acatccaggc atcgccactg ccatagcgct ggttgagtaa
     2401 ataagcgaca aacgccaatg ctgcagcgag ggtacagcca cgagacggcg tggtaaacaa
     2461 ggctttaaga tgagggaaaa tgaaagccag tgcgccgagg ataaagaacg gtaaataaaa
     2521 tagcgtttgc atgacaataa aattgaacat gccattactc agaatgggtg gatacacaat
     2581 aaaaatcgtt cttcttatta ccgcatagcc gatgccgagg cataaaaaaa tcaccgatag
     2641 ttttaccatc gagaattttt tattcgtttt atcagaattt tctaaattat ttctgatgcg
     2701 cttaaatatc catacacaca gcgtcgtcat gactactaac accagtaaaa accacaggtg
     2761 tgatattaat tcccaggcca acgtattata tttgtcatac aatgatagcc caggccaact
     2821 ttccgctttc cctttgacat attgcagcat aataaattgc ggtaatgtca gtagggggat
     2881 ggctgttaac atcgggatac ctacacgttc gacacgtact ttccaccatt ttttcaaggg
     2941 atagcgtaaa aaaagcatgt aggaaaagta tccggatata acgaaaaata cctgcatgcg
     3001 gaacgagtgg atgaagtcat taaaaagggt tagccacaat gacggttcgg cgctattcac
     3061 atgccatgta tggctcgaat agattaaaga aatatgaaaa gggatcccta acaacatcag
     3121 ccaggcgcgg atggagtcga ggaaatattc acgttgcgcg ggtactggat tcatatatgg
     3181 ttaactaatc tcggattttt cgtcttatcc ctgtcgggtt atgcctttag gcttgttgcc
     3241 atagcgacac cgacctgacc gcgccaggcg caggcttcaa ggtttttatg catagcatca
     3301 tcgctaccac taaccagaat ggaagcgtct gtaagacggt tgataaataa atttgctggc
     3361 aaaccctaca cgaagtcgat gcttctgtct ttaggagaag cacggaaagt gaaaacggtt
     3421 gcaatcaggt gcttaatcca tgagccagcg tgctgaacga taccgggatt cagttgtcgg
     3481 aatggctggt tatccattaa aatagatcgg atcgatataa gcacacaaag ggggaagtgc
     3541 ttactaatta tgaaacataa actacaaatg atgaaaatgc gttggttgag tgctgcagta
     3601 atgttaaccc tgtatacatc ttcaagctgg gctttcagta ttgatgatgt cgcaaagcaa
     3661 gctcaatcct tagccgggaa aggctatgag gcgcctaaaa gcaacttgcc ctccgttttc
     3721 cgcgacatga aatacgcgga ctatcagcag atccagttta atcatgacaa agcgtactgg
     3781 aacaatctga agaccccatt caaactcgag ttctaccatc agggtatgta cttcgatacc
     3841 ccggtcaaaa taaatgaagt gactgccacc gcagtcaaac gaatcaaata cagcccggat
     3901 tatttcactt tcggcgatgt tcagcatgac aaagacacgg taaaagacct tggttttgcc
     3961 ggttttaaag tgctttaccc gatcaacagc aaagataaaa acgatgaaat cgtcagcatg
     4021 ctcggggcca gctatttccg cgtgattggt gcaggtcagg tttatggcct ttctgcccgc
     4081 ggcctagcaa ttgataccgc tttgccatcg ggtgaagaat ttccacgctt caaagagttc
     4141 tggatcgagc gtccaaaacc gactgataaa cgtttaacca tttatgcatt gcttgactcg
     4201 ccgcgtgcga caggtgctta caaattcgtg gttatgccag ggcgtgacac ggttgtggat
     4261 gtgcagtcga aaatctatct gcgcgataaa gtcggcaaac tgggggttgc accgttaacc
     4321 agtatgttcc tgtttgggcc gaaccaaccg tcgcctgcaa ataactatcg tccggagttg
     4381 cacgactcta acggtctgtc tatccatgct ggtaatggcg aatggatctg gcgtccgttg
     4441 aataacccga aacatttagc ggtcagcagc ttctccatgg aaaacccgcg aggctttggt
     4501 ctgttgcaac gaggtcgtga tttctcccgc tttgaagatc tcgatgatcg ttacgatctc
     4561 cgtccaagcg catgggtgac tccgaaaggg gagtggggca aaggcagcgt tgagctggtg
     4621 gaaattccaa ccaacgatga aaccaacgat aacatcgtcg cttactggac gccggatcag
     4681 ctgccggagc cgggtaaaga gatgaacttt aaatacacca tcaccttcag ccgtgatgaa
     4741 gacaaactgc atgcgccaga taacgcatgg gtgcaacaaa cgcgtcgttc aacgggggat
     4801 gtgaagcagt cgaacctgat tcgccagcct gacggtacta tcgcctttgt ggtcgatttt
     4861 accggcgcag agatgaaaaa actgccagag gataccccgg tcacagcgca aaccagcatt
     4921 ggtgataatg gtgagatagt tgaaagcacg gtgcgctata acccggttac caaaggctgg
     4981 cgtctggtga tgcgtgtgaa agtgaaagat gccaagaaaa tcactgaaat gcgtgctgcg
     5041 ctggtgaatg ccgatcagac gttgagtgaa acctggagct accagttacc tgccaatgaa
     5101 taagacaact gagtacattg acgcaatgcc catcgccgca agcgagaaag cagcattgcc
     5161 gaagactgat atccgcgccg ttcatcaggc gctggatgcc gaacaccgca cctgggcgcg
     5221 ggaggatgat tccccgcaag gctcggtaaa ggcgcgtctg gaacaagcct ggccagattc
     5281 acttgctgat ggacagttaa ttaaagacga cgaagggcgc gatcagctta aggcgatgcc
     5341 agaagcaaaa cgctcctcga tgtttcccga cccgtggcgt accaacccgg taggccgttt
     5401 ctgggatcgc ctgcgtggac gcgatgtcac gccgcgctat ctggctcgtt tgaccaaaga
     5461 agagcaggag agcgagcaaa agtggcgtac cgttggtacc atccgccgtt acattctgtt
     5521 gatcctgacg ctcgcgcaaa ctgttgtcgc gacctggtat atgaagacca ttcttcctta
     5581 tcagggttgg gcgctgatta atcctatgga tatggttggt caggatttgt gggtttcctt
     5641 tatgcagctt ctgccttata tgctgcaaac cggtatcctg atcctttttg cggtactgtt
     5701 ctgttgggtg tccgccggat tctggacggc gttgatgggc ttcctgcaac tgcttattgg
     5761 tcgcgataaa tacagtatat ctgcgtcaac agttggcgat gaaccattaa acccggagca
     5821 tcgcacggcg ttgatcatgc ctatctgtaa cgaagacgtg aaccgtgttt ttgctggctt
     5881 gcgtgcaacg tgggaatcag taaaagccac cgggaatgcc aaacactttg atgtctacat
     5941 tctgagtgac agttataacc cggatatctg cgtcgcagag caaaaagcct ggatggagct
     6001 tatcgctgaa gtcggtggcg aaggtcagat tttctatcgc cgccgccgtc gccgcgtgaa
     6061 gcgtaaaagc ggtaatatcg atgacttctg ccgtcgctgg ggcagccagt acagctacat
     6121 ggtggtgctg gatgctgact cggtaatgac cggtgattgt ttatgcggcc tggtgcgctt
     6181 gatggaagcc aacccgaacg ccgggatcat tcagtcgtcg ccgaaagcgt ccggtatgga
     6241 tacgctgtat gcgcgctgtc agcagttcgc gacccgcgtg tatgggccac tgtttacagc
     6301 cggtttgcac ttctggcaac ttggcgagtc gcactactgg ggacataacg cgattatccg
     6361 cgtgaaacca tttatcgagc actgcgcact ggctccgctg ccgggcgaag gttcctttgc
     6421 cggttcaatc ctgtcacatg acttcgtaga agcggcgttg atgcgccgtg caggttgggg
     6481 ggtctggatt gcttacgatc tcccgggttc ttatgaagaa ttgccgccta acttgcttga
     6541 tgagctaaaa cgtgaccgcc gctggtgcca cggtaacctg atgaacttcc gtctgttcct
     6601 ggttaagggt atgcacccgg ttcaccgtgc ggtgttcctg acgggcgtga tgtcttatct
     6661 ctccgctccg ctgtggttta tgttcctcgc gctctctact gcattgcagg tagtgcatgc
     6721 gttgaccgaa ccgcaatact tcctgcaacc acggcagttg ttcccggtgt ggccgcagtg
     6781 gcgtcctgag ctggcgattg cactttttgc ttcgaccatg gtgctgttgt tcctgccgaa
     6841 gttattgagc attttgctta tctggtgcaa aggaacgaaa gaatacggcg gcttctggcg
     6901 cgttacatta tcgttgctgc tggaagtgct gttttccgtg ctgctggctc cggtacgcat
     6961 gctgttccat acggtcttcg ttgtcagcgc gttccttggc tgggaagtgg tgtggaattc
     7021 accgcagcgt gatgatgact ccacttcatg gggcgaagcg ttcaaacgcc acggctcaca
     7081 gctgctgttg gggttagtgt gggctgttgg gatggcgtgg ctggatctgc gtttcctgtt
     7141 ctggctggca ccgattgtct tctcgttgat cctgtcaccg tttgtttcgg tgatttccag
     7201 ccgtgccacc gttggtctgc gcaccaaacg ctggaaactg ttcctgatcc cggaagagta
     7261 ttcaccgccg caggtgctgg ttgataccga tcggttcctt gagatgaatc gccaacgctc
     7321 ccttgacgat ggctttatgc acgcagtgtt taacccgtca tttaacgctc tggcaaccgc
     7381 aatggcgaca gcgcgtcacc gcgccagtaa ggtgctggaa atcgcccgtg accgccacgt
     7441 tgaacaggcg cttaacgaga cgccagagaa gctgaatcgc gatcgtcgcc tggtgctgct
     7501 aagcgatccg gtgacgatgg cccgtctgca tttccgcgtc tggaattccc ctgaaagata
     7561 ttcttcatgg gtgagttatt acgaagggat aaagctcaat ccactggcat tgcgtaaacc
     7621 ggatgcggct tcgcaataaa aacgtagttg cctgatgcgc tacgcttatc aggcctacat
     7681 cgttcctgca atttattgat tttgcaagat tttgtaggtc ggataaggcg ttcacgccac
     7741 atccggcaaa aacaacgagc caataaaaat accggcgtta tgc
//
LOCUS       DAJVRP010000145         7744 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVRP010000145 DAJVRP010000000
VERSION     DAJVRP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.145"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..1014
                     /gene="ydfJ"
                     /locus_tag="OQN12_003675"
     CDS             <1..1014
                     /gene="ydfJ"
                     /locus_tag="OQN12_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCS1655855.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1103..2563
                     /locus_tag="OQN12_003676"
     CDS             1103..2563
                     /locus_tag="OQN12_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1655856.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="OQN12_003677"
     CDS             complement(2599..2802)
                     /gene="ydfZ"
                     /locus_tag="OQN12_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1655857.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2980..3666)
                     /gene="ydfH"
                     /locus_tag="OQN12_003678"
     CDS             complement(2980..3666)
                     /gene="ydfH"
                     /locus_tag="OQN12_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCS1655858.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="OQN12_003679"
     CDS             complement(3755..4501)
                     /gene="ydfG"
                     /locus_tag="OQN12_003679"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1655859.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4638..6683
                     /gene="dcp"
                     /locus_tag="OQN12_003680"
     CDS             4638..6683
                     /gene="dcp"
                     /locus_tag="OQN12_003680"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1655860.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6728..7246)
                     /locus_tag="OQN12_003681"
     CDS             complement(6728..7246)
                     /locus_tag="OQN12_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCS1655861.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7522..>7744
                     /gene="ydeI"
                     /locus_tag="OQN12_003682"
     CDS             7522..>7744
                     /gene="ydeI"
                     /locus_tag="OQN12_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCS1655862.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1942 a         1812 c         1954 g         2036 t
ORIGIN      
        1 atcttgctgg tgacgctgcg tattattcag ggattgggtg caggtgcgga aatttccggt
       61 gccggtacga tgctggcgga atatgcgcca aaaggtaagc gcggaattat ctcctcattt
      121 gtggctatgg gaactaactg cggcacctta agcgcaacgg caatctgggc ctttatgttc
      181 ttcattctca gtaaagagga actgctggcg tggggatggc gtataccgtt cctagcgagc
      241 gttgtcgtga tggtctttgc tatctggttg cgtatgaatc tgaaagaaag cccggtcttt
      301 gagaaggtta acgacagcaa ccaaccgaca gcaaaacctg cacctgctgg tagcatgttc
      361 cagagcaaat ccttctggct ggcaacaggg ctgcgttttg gtcaggcggg taactccggt
      421 ttaattcaga ctttccttgc aggctattta gtacagacgt tattgtttaa caaagcaatt
      481 ccaacagatg cattgatgat cagttcgatt ctcggcttta tgaccattcc gttccttggt
      541 tggttatccg ataaaattgg tcgccggatc ccgtatatta ttatgaatac ctctgcgatt
      601 gtgctggcgt ggccaatgct ttctatcatc gtagataaaa gctatgcccc gagcaccatt
      661 atggttgcac tgattgtgat tcataactgt gcggtgctgg gattatttgc tctggaaaac
      721 attaccatgg cagaaatgtt cggctgtaaa aaccgcttca cccggatggc tatttcaaaa
      781 gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc tggcgggtat tttctgcacc
      841 atgacggaat cctggtatcc gatcgccatt atgatcatgg catattcagt gattggttta
      901 atctctgcgc tgaaaatgcc agaggtgaaa gaccgtgatt taagtgcgct ggaagacgct
      961 gcggaagatc aaccgcgtgt tgtaagagct gcgcaacctt ccagaagtct gtaaaccctc
     1021 aatcccttct cttaccggag aggggatttt tattcatata acaaaacata tagcttgcca
     1081 tatttaaatt taaggaattg ttatgggaaa taatttgtta tcagcaaaag cgacactccc
     1141 tgtttatgat cgtaataacc tggccccaag aattgttcat ttaggctttg gtgcatttca
     1201 ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca gaacatttca gtgactgggg
     1261 atattatgag gtcaacttaa tcggcggcga acagcaaatt gccgatttac aacagcaaga
     1321 taatctttat accgttgcgg aaatgtcggc cgatgcgtgg acggctcgcg tcgttggcgt
     1381 cgttaaaaaa gccttgcacg tacagatgga tggcttagaa accgtgttgg ctgcgatgtg
     1441 tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa aaagggtatt tccactctcc
     1501 ggcgaccgga cagttaatgc tcgagcaccc gatggtcgct gccgacgtgc aaaatcccca
     1561 ccagccgaaa acagcaacag gggtgattgt tgaggcgctg gctcgccgta aagcggcagg
     1621 acttcccgca tttaccgtca tgtcatgtga caacatgcca gaaaacggtc atgttatgcg
     1681 tgacgttgtc acttcctacg cgcaagccgt tgatgtaaaa ctggcacaat ggatcgaaga
     1741 taacgtgact ttcccatcaa caatggtgga ccgtattgtg cccgcagtga cagaggatac
     1801 gctggcgaaa atcgaacaac ttaccggtgt gcgcgatcct gctggcgttg cctgtgaacc
     1861 tttccgccag tgggttatcg aagataactt tgtagccgga cgtccggaat gggaaaaagc
     1921 gggagccgaa ctggttagtg atgtgctgcc ttatgaagag atgaagttgc gcatgctcaa
     1981 cggcagtcat tcattcctgg catatctggg ttatcttgct ggatatcagc acattaatga
     2041 ctgtatggaa gatgaacatt atcgtcatgc ggcgtatggc atgatgttgc aggaacaagc
     2101 gccgacgctg aaagtgcagg gcgttgattt gcaagattac gctaaccgat taattgcacg
     2161 ctatagcaac ccggcgttac gtcatcgaac ctggcagatt gcgatggatg gcagccagaa
     2221 attgccacag cggatgttgg attctgttcg ctggcatctg gcgcatgaca gcaagttcga
     2281 tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc ggtggtgttg atgaacaggg
     2341 aaatccgata gaaatcagtg acccactgtt acctgttatt cagaaggctg tacaaagtag
     2401 tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt aaggcaatct ttggtgatga
     2461 tttgccagac aatagcttgt ttactgcaaa agtgacggaa gcgtacttgt ctttattagc
     2521 gcatggcgcg aaagcgaccg tggcgaaata ttccgtgaag taaaaaaagc aatgccgcca
     2581 tcggcggcat tcacacactt agttcatgcc gagacgaatc aggtccagag gtgccagttt
     2641 ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag cggtcagacc
     2701 ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca taacacggct
     2761 gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt tctcctttct
     2821 gatttacagt cgaagcccac gtaacgggcg caatataaat actcctgtcg agtgaaatat
     2881 tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccgt aaggcggggg
     2941 gaaaagcctc tcatcccgga tgggatgaga gtaaatgaat tactcttcgc taaaccagtc
     3001 gctgttttcc tggcggatct ggcgcacgga ttcgctgatt tcctgcaaat gttgcgtcat
     3061 tgcacgttct accgcatcgc catcacgttt ttgcagggca gagaaaatat caagatgctg
     3121 gcgtaacagc atttctggtg gagaaacatg gtcgaaactc atatagcgca cgcgatcaac
     3181 ggtcgctttc aggttctcaa tggtatccca cgccagttga cagtcggcaa tctgcgtcag
     3241 gagttgatgg aagttgtcat caagttcaaa aaaatcatcc agttgcttgc gctcaatggc
     3301 aatgcgttgc tggtgaagat tttgttccag ttggtagcac tggctttcgg taatcatgct
     3361 cgccgcccga cgcgccaccg cgcactcaat ggcctgacgg ataaaactgc cgttgcgcac
     3421 ctgggccatg gaaattttgt tgacgtagct gccacgctgc ggacgaattt gaatcaggcc
     3481 gttttccgcc agtttaataa aggcttcacg aaccggctgg cgtgacacat tgaaacgaac
     3541 agaaacttct ttttccgaca acggtgtgcc tggtgcaatc aggcaatgga caatgtcgcg
     3601 acgaagaata cgataaatct gctgattgac aggctgtgtg ggattaagtt gcgtttcgac
     3661 ggtcattcgt tcttgcttgt gagtgagtta actgcatgag tctaccactt ttttgtgaca
     3721 agcaataacc cggcagttac gccgggtata aaaattactg acggtggaca ttcagtccgg
     3781 catagctttg ggtaaccggc atcatttcca gggtattgat attgacgtga gcaggcagcg
     3841 ttgacaccca ccagacggct tcgctgacat cttctggcgt caatgcaacg gtattttgat
     3901 aggttttttc cgctttaccg tcatcgcctt taaagcggac attggaaaac tcggtgccac
     3961 ccaccagacc cggttcgatg tcggtgacgc gcaccgccgt accatgcaga tccgtacgca
     4021 gattcaggct aaactgacga acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat
     4081 acggccagct gcctgccgtt gagccaatgt taataatatg accatgatta cgttcaacca
     4141 tacccggtaa gacggcgcgc gtcatatata ctaggccttt gttgttggta tcaatcatcg
     4201 tttcccagtc ttcaacgctg gctttatgcg caggctccat gcccaacgcc aggccggcat
     4261 tatttaccag gatatcaata ttgctccact cggcagggag cgatgccagc atctcttcaa
     4321 tagcggcgcg gttgcgaacg tccagttggg cgatatacag attatctccc agttcgtctg
     4381 ttaactcctg caaccgctcc tggcgacggc cagtggcgat aactttatgc ccttgttgaa
     4441 taaaacgacg agtaatgcat tcaccaaaac ctgccgttgc tccagttact aaaacgatca
     4501 tctcactgtt cctcaacgct tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg
     4561 gtaatactgg tttgcaagat tgcactccgt tatcagtaag cctacgctat ggaacacctc
     4621 gcgttcagga gaagaaaatg acaacaatga atcctttcct tgtgcaaagc acactgccgt
     4681 atctggctcc tcattttgat caaattgcca atcatcacta tcgcccggca ttcgatgagg
     4741 gaatgcaaca aaagcgggca gaaattgctg ccatcgcgct taacccgcaa acgcctgatt
     4801 tcaacaatac tattctggca ctggaacaaa gcggagaatt aattacccgc gttaccagcg
     4861 tcttttttgc gatgactgcg gcgcatacca atgatgaatt acagcgtctt gatgaacagt
     4921 tttccgctga actggcggaa ctggctaatg atatctatct gaacggtgaa ttattcgcgc
     4981 gggtagatgc tgtctggcag ctccgtgaat ccctggggct tgatagtgaa tccatccgcc
     5041 tggtggaggt tattcatcaa cgttttgtcc ttgccggagc caaacttgca caagctgata
     5101 aagcaaaatt aaaagtactg aatacagaag ctgcgaccct gaccagccag tttaaccagc
     5161 ggttacttgc agcaaataaa tccggcggtc tggttgtgaa cgatatcgcg cagctggcag
     5221 gaatgagtga gcaagagatt gcgctggcgg cagaggcggc tcgcgagaaa ggtctggata
     5281 acaaatggct gattccgctg ctgaatacca cccaacaacc ggcgcttgcc gaaatgcgcg
     5341 atcgtgcgac gcgtgaaaaa ctgtttattg cgggctggac gcgagcggaa aaaaatgatg
     5401 ccaatgatac ccgcgctatc attcaacgtc tggtagagat tcgcgcacag caggcgaaac
     5461 tgcttggttt tcctcattat gccgcatgga aaatcgccga tcagatggca aaaacgccag
     5521 aagcagcact caactttatg cgggaaattg ttccagcggc gcgtcaacgt gcgagcgatg
     5581 aattagcctc catacaggcg gttatcgata agcagcaggg cgggtttagc gcgcagccgt
     5641 gggactgggc attttatgcg gaacaggtac ggcgggagaa atttgatctt gatgaggcgc
     5701 agctcaagcc atattttgaa ttaaacacgg tgttaaatga aggtgtattc tggaccgcga
     5761 atcagctctt cggtattaag tttgtcgaac gttttgatat tccggtctac catcctgacg
     5821 ttcgtgtgtg ggaaattttt gatcataatg gcgtgggact ggcgttattt tacggtgatt
     5881 tcttcgcccg tgattcaaaa agcggcggtg catggatggg caattttgtt gagcaatcaa
     5941 cgcttaatga aacgcatccg gtaatttata acgtctgcaa ttatcagaaa cccgctgccg
     6001 gtgagcctgc gttgttactc tgggatgatg taataacctt attccatgaa tttggtcata
     6061 cgctgcacgg cctttttgcc cgccagcgtt atgccacgct ttccggcacc aacacgccgc
     6121 gtgattttgt cgaatttccg tcgcaaatca acgaacactg ggcaacgcat ccgcaggtat
     6181 tcgctcgcta cgcccggcat tatcagagcg gggcagcaat gcctgacgaa ctgcaacaga
     6241 aaatgcgtaa tgccagcctg ttcaacaaag ggtatgagat gagcgaactg cttagcgccg
     6301 cacttctcga tatgcgctgg cattgcctgg aagaaaacga agcaatgcag gatgtcgatg
     6361 attttgaatt gcgggcgctg gtggcggaaa atatggatct tcctgctata ccgccacgct
     6421 atcgcagcag ttatttcgcc catatttttg gtggcggata tgctgcaggt tattacgctt
     6481 atctgtggac gcaaatgttg gccgatgatg gttaccagtg gtttgttgag cagggcggat
     6541 taacgcgtga aaatgggcag cgttttcgcg aggcgatcct ttccagaggt aacagcgaag
     6601 atctggaacg cctgcatcga caatggcgtg gtaaggctcc tcagattatg ccgatgctgc
     6661 aacatcgtgg cttgaacata taaacaccac caatggctgg cctggagata tcgccaggcc
     6721 agcctggtta tagcagtaat tgcagcagct gggcgagggc aaacctcacc gctaaagcta
     6781 ataccgtttc gcagtcgcca gcaaaatgca taaccgcagt gtagttctgg cctttaatat
     6841 gccacgcgaa ccagacggta cccgctggcg taccatcttc accgccctcc ggtccgccgt
     6901 agccggtaat ggcaatactg acatcagcat ccgcacgctc tatggcacct gttgccattt
     6961 ctgctgccac tttctcactc accgcagaat atcgttcaag agactgctgg cttacgctga
     7021 ggattttcat ctttgcctga tcggtgaaag taacaaagcc tgcaccgtaa aatttgggtg
     7081 tatcttcagc tgcacacagg gcgctagcca acttaccgcc ggtgcatgat tcggcggtag
     7141 ttaagcgtaa ttgatccgcg ataagtcttt gactcaacgc ggatgtcagg ttttcaatag
     7201 tgtcggtatc tgcgagttgt acaattttat ctttattaat gttcactgtt ttttgtccac
     7261 gctattatat gtaaaggtga tttgcttttt ctgtatgctc gtccaatata ataattttca
     7321 aacatataaa tccgattatt ctgaaaacat gtttaagact cttcaatctt cttaacttta
     7381 gtgagtcaaa ttctctgaat atttgacagc tagtgggctg attttaaatg ccttataaca
     7441 attggcagta ttcttatttg gcaattataa aaatagcaac cagacttaat aagtcaaacc
     7501 aaacgcaatt aaggagttag tatgaaattt cagtccattg tattagcaag ttttcttgtc
     7561 atgccttatg cgttggcaga tgaccagggc ggtttaaaac aggatgcagc gccaccgccg
     7621 cctcatgcaa tagaagatgg ctatcgcgga accgatgatg caaaaaaaat gaccgttgat
     7681 ttcgcaaaaa acatgcacga tggcgcttcg gtttcactac gcggtaattt gatttctcac
     7741 aaag
//
LOCUS       DAJVRP010000146         7555 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVRP010000146 DAJVRP010000000
VERSION     DAJVRP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.146"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..1159
                     /gene="rbsA"
                     /locus_tag="OQN12_003683"
                     /pseudo
     CDS             1..1159
                     /gene="rbsA"
                     /locus_tag="OQN12_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1164..2129
                     /gene="rbsC"
                     /locus_tag="OQN12_003684"
     CDS             1164..2129
                     /gene="rbsC"
                     /locus_tag="OQN12_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1655863.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2154..3044
                     /gene="rbsB"
                     /locus_tag="OQN12_003685"
                     /pseudo
     CDS             2154..3044
                     /gene="rbsB"
                     /locus_tag="OQN12_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3170..4099
                     /gene="rbsK"
                     /locus_tag="OQN12_003686"
     CDS             3170..4099
                     /gene="rbsK"
                     /locus_tag="OQN12_003686"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1655864.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4103..5095
                     /gene="rbsR"
                     /locus_tag="OQN12_003687"
     CDS             4103..5095
                     /gene="rbsR"
                     /locus_tag="OQN12_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1655865.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OQN12_003688"
     CDS             complement(5061..6488)
                     /gene="mdtD"
                     /locus_tag="OQN12_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1655866.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6511..7203)
                     /locus_tag="OQN12_003689"
     CDS             complement(6511..7203)
                     /locus_tag="OQN12_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1655867.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1923 a         1828 c         1987 g         1817 t
ORIGIN      
        1 aaaccatgta tgtcgaagcg gataaattgc tggctaaact taacctgcgc tttaaaagcg
       61 acaagttagt gggcgatctt tccatcggtg accagcaaat ggttgaaatc gccaaagtgc
      121 tgagctttga gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg accgataccg
      181 aaaccgaatc cctgttccgc gtcatccgcg agctgaaatc gcaaggccac tgtattgtct
      241 atatctccca ccgcatgaaa gaaatcttcg agatttgcga tgacgttacc gtttttcgtg
      301 atgggcaatt tattgctgag cgcgaagtgg catcactgac cgaagattcg ctgattgaga
      361 tgatggtggg ccgcaagctg gaagatcaat atccacacct ggacaaagcg ccaggagata
      421 tccgcctgaa agtcgataat ctctgcggac ctggcgttaa cgatgtctct tttactttac
      481 gcaaaggcga aattcttggc gtctctggtt tgatgggcgc aggtcgtacc gaactgatga
      541 aagtgctcta cggcgcactg ccgcgcacca gcggttacgt caccctggat gggcatgaag
      601 tcgttacccg ttcaccgcag gatggcctgg caaacggcat tgtgtatatc tccgaagacc
      661 gtaaacgtga cggtttagtg ctgggcatgt cagtaaaaga gaacatgtcg ctgacagcgc
      721 tgcgctactt cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag caggctgtga
      781 gtgatttcat tcgtctgttt aatgtgaaaa cgccgtcgat ggaacaggca attggtctgc
      841 tttccggtgg caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca cccccaaagt
      901 attgatcctt gatgagccta cccgtggcgt agatgtcggc gcgaaaaaag agatctatca
      961 actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat cggagatgcc
     1021 agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc tcagcgggga
     1081 atttactcgt gagcaggcca cccaggaagt gttaatggct gtcgctgtgg gcaagcttaa
     1141 tcgcgtgaat caggagtaaa aaaatgataa cccagactgt ctctggtcgc cgttatttca
     1201 cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg atcgcgattg
     1261 tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt ctccagcaaa
     1321 cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg tcgggcatag
     1381 acttgtcggt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca
     1441 ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc gcaattggtg
     1501 cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct acgctggtta
     1561 tgatgctttt actgcgcggc gtgactatgg tttataccaa cggtagccca gtgaataccg
     1621 gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac
     1681 cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg ctgcatcaca
     1741 cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg cgtctttctg
     1801 gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg ctggcatcgc
     1861 tggccgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg gggactggct
     1921 atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg ggcggaaaag
     1981 gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat aatggattga
     2041 atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg attttgctgg
     2101 cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg aatatgaaca
     2161 tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg
     2221 cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg ttctttgtat
     2281 cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg gtggtgctgg
     2341 actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc gttcgcggca
     2401 ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct gtgaagatgg
     2461 ctaactaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg
     2521 tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat tacatcgcga
     2581 agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt acatccgcag
     2641 cccgtgaacg tggcgaaggc ttccagctgg ccgttgctgc tcacaagttt aatgttcttg
     2701 ccagccagcc agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga
     2761 ccgctcatcc ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc
     2821 tgcgcgcact gcaaactgcc ggtaaatcgg atgtgatggt cgtcggattt gacggtacac
     2881 cggatggcga aaaagcggtg aatgatggca aactagcagc gactatcgct cagctacccg
     2941 atcagattgg cgcgaaaggc gttgaaaccg cagataaagt gctgaaaggc gagaaagttc
     3001 aggctaagta tctggttgat ctgaaactgg ttgttaagca gtagttttaa tcaggttgta
     3061 tgacctgatg gtgacataaa tacgtcatcg acagatgaac gtgtaatata aagaaaagca
     3121 gggcacgcgc caccctaaca cggtggcgca ttttatggac atcccgaata tgcaaaacgc
     3181 aggcagcctc gttgttcttg gcagcattaa tgctgaccac attcttaatc ttcaatcttt
     3241 tcctactcca ggcgaaaccg taaccggtaa ccactatcag gttgcatttg gcggcaaagg
     3301 cgcgaatcag gctgtggctg ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac
     3361 tggcgatgac agcattggcg agagcgttcg ccagcagctc gccactgata acatcgatat
     3421 tactccggtc agcgtgatca aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg
     3481 cgaaggtgag aatgtcatcg gtattcatgc cggcgctaat gctgctcttt ccccatcgct
     3541 ggtggaagcg caacgtgagc gtattgccaa cgcgtcagcg ttattaatgc agctggaatc
     3601 accactcgaa agtgtgatgg cagcggcgaa aatcgcccat caaaataaga ctatcgttgc
     3661 gcttaacccg gctccggctc gcgaacttcc tgacgaactg ctggcgctgg tggacattat
     3721 tacgccaaac gaaacggaag cagaaaagct caccggtatt cgtgttgaaa atgatgaaga
     3781 tgcagcgaag gcggcgcagg tactgcatga aaagggtatc cgtactgtac tgattacttt
     3841 aggaagtcgt ggtgtatggg ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg
     3901 ggtgcaggct gtcgatacca ttgctgccgg agataccttt aacggcgcgt taatcacggc
     3961 gttgctggaa gaaaaaccat tgccagaggc gattcggttt gcccatgctg ccgctgcgat
     4021 tgccgtaaca cgtaaaggcg cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt
     4081 tttagacagg cagaggtgac gcttggctac aatgaaagat gttgcccgtt tggcgggcgt
     4141 ttctacctca acagtttctc acgttatcaa taaagatcgc ttcgtcagtg aagcgattac
     4201 cgccaaagtt gaagcggcga ttaaagaact caattacgcg ccatcagctc tggcgcgtag
     4261 cctcaaactc aatcaaacac ataccattgg catgttgatc actgccagta ccaatccttt
     4321 ctattcagaa ctggtgcgtg gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt
     4381 cctttgcaat accgaaggcg atgaacagcg gatgaatcgc aatctggaaa cgctgatgca
     4441 aaaacgcgtt gatggcttac tgttactgtg taccgaaacg catcagcctt cgcgtgaaat
     4501 catgcaacgt tatccgacag tgcctaccgt gatgatggac tgggctccgt tcgatggcga
     4561 cagcgatctt attcaggata actcgttgct gggcggagac ttagcaacgc aatatctgat
     4621 cgataaaggt catacccgta tcgcctgtat taccggtccg ctggataaaa ctccggcgcg
     4681 cctgcggttg gaaggttatc gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg
     4741 ctatgaagtc actggtgatt ttgaatttaa cggcgggttt gacgccatgc gccaactgtt
     4801 atcacatccg ctgcgtcctc aggccgtctt taccggaaat gacgctatgg ctgttggcgt
     4861 ttaccaggcg ttatatcagg cagagttaca ggttccgcag gatatcgcgg tgattggcta
     4921 tgacgatatc gaactggcaa gctttatgac gccaccatta accactatcc accaaccgaa
     4981 agatgaactg ggggagctgg cgattgatgt actcatccat cggataaccc agccgaccct
     5041 tcagcaacaa cgattacaac ttactccgat tctgatggaa cgcggttcgg catagattta
     5101 cgctgtcttt tgatcaaatt attaccatcg gttgttttca gaagcatgaa cattgctgct
     5161 gaagcaacag taataatgcc catcgtgata aacgtatagt ggaattgttc gacagtcgtt
     5221 gtgccttcca tcccttcata aatgcgaagg acggccgcac ttacagcaac gcctaaacta
     5281 atcgacagtt gctgcgtgac cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc
     5341 agatcggcaa gtgtgatggt attcatcgcg gtaaactgcg tcgacatagc catccctaat
     5401 ataaacaacg gcaagatcag catccatatc gccattgccg gtgattgcaa agagaactga
     5461 gcgatcatta gcccaataat caccgtgatc cccactaatg tatggcgata gcccagacga
     5521 cgtaagactt gggtaaccat cgattttgca ataatggaac ctaacgctgt cggtgccatc
     5581 atacagccgg caataaacgc ctgataacca aatcctacct gtaacatcaa tggcataagg
     5641 aacggtacac agccggtccc cagacgggtt gcaatattgc ctacgatacc gatcgagaaa
     5701 gtgcgggttt taaataaatc taatgaaatt aatgggtttg gcgtacgtcg cgcatggaga
     5761 atatagagaa gcagtaaccc gatgctggtg acaattaccg tcaaggcaat ccagctggcg
     5821 acaatctttt ccccgaatag ctctattccg cttgaaaaga gaacaaggct gaggccaaac
     5881 agcaaaaagc cagtgatatc gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg
     5941 cgcgcgtaaa gaaggcccgc aatacctatg gggatattaa ttaaaaatat ccagtgccag
     6001 gttgcccagg tgaccagcac gccgccaaga acggggccta aaattggccc caccagaccc
     6061 ggcatggcga caaaattcaa taccggaaga agttcattac gaggataagc acgcagtaag
     6121 gccggccgag caacaggcat catcattgcg tcgcctatcc cttgaataac ccggaagaca
     6181 accagctgtg gtagcgaatt agaaagtgcg taggccagag aacccaacgt gaacagactc
     6241 acggcaaggg taaaaatgcg acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga
     6301 ataagcatcg ccaccgtcag cgtataactg atgatggctg attgcatcgc gagaggagaa
     6361 cgattaaggc tatgagcgat tgcgggtaag gcggtattca gaatagtggc atcaagtgcc
     6421 tgcatgaaga aggccatcgc cgcgatccac ggcaaacccg acatactacg cttcttttta
     6481 tcgctcattc aatgtcctgt tatcgggtta tcacttatca ggtgagcgta gcagcgcctg
     6541 acaagcttta aatgccgcgt cgccatcgct ttggataatc gcatcgacaa tcgcctggtg
     6601 cagatccagc tttatcactg tgtcgctggt aattgacgtg aagtaagtgt gataaaccga
     6661 atggaatagc gaggcgaatg aggtcaaaaa cggattggcg ctcatttcat agatatgctc
     6721 atgccaggcc atatcgactt cgatccagcg ttcacggcga aagttctctt ttaatgccgc
     6781 catttcggcc attaacgtat tgagatgcgc cttctgttcc gcggtgccaa ccgttgctgc
     6841 cagtaggcag gcttgcggct ccagacagat acgcataacc agaaagtgat cgatgacctg
     6901 atgaaagttc tcttctgtta tccaccaagt aagcaattcc tgatcaagaa aattccagtt
     6961 cgattgtggc atgacccgag taccaattcg cggtcgcggt aaaaccatcc cttttgccgt
     7021 taacgttttg accgcttcgc gtaccgctgt acgactcact ccaaattgct cgcccagctc
     7081 aatctcaccg ggcaaaatgg tgccgggttc atattcacct tttaagatcc gttgcgccag
     7141 cttctcagcc agaacatacg aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg
     7201 cattacttat cttccttttt ctttttattc ctccttagta tgccaccagg aagtgtgatt
     7261 acggttgcaa aaacggcaaa ttgcttgttt tatggcacat taacggggta ttttgctgaa
     7321 aaaatgcgcg gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta
     7381 taatacgcca ccactgacac ggaacaacgg caaatacgcc gccgggtcag cggggttctc
     7441 ctgagaattc cgacagagaa agcaaaaata aatgattgac tctgtagcgg gaaagcgtat
     7501 tatgcacacc cccgcgccgc tgagaaaaag cgaagcgcac tgctctttaa caatt
//
LOCUS       DAJVRP010000147         7250 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVRP010000147 DAJVRP010000000
VERSION     DAJVRP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.147"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(220..600)
                     /locus_tag="OQN12_003690"
     CDS             complement(220..600)
                     /locus_tag="OQN12_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1655868.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(593..793)
                     /locus_tag="OQN12_003691"
     CDS             complement(593..793)
                     /locus_tag="OQN12_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCS1655869.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(888..1145)
                     /locus_tag="OQN12_003692"
     CDS             complement(888..1145)
                     /locus_tag="OQN12_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655870.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(1142..2533)
                     /locus_tag="OQN12_003693"
     CDS             complement(1142..2533)
                     /locus_tag="OQN12_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1655871.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPLMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(2530..3408)
                     /locus_tag="OQN12_003694"
     CDS             complement(2530..3408)
                     /locus_tag="OQN12_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1655872.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(3419..4327)
                     /locus_tag="OQN12_003695"
     CDS             complement(3419..4327)
                     /locus_tag="OQN12_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1655873.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(4314..4547)
                     /locus_tag="OQN12_003696"
     CDS             complement(4314..4547)
                     /locus_tag="OQN12_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655874.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(4544..5560)
                     /locus_tag="OQN12_003697"
                     /pseudo
     CDS             complement(4544..5560)
                     /locus_tag="OQN12_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            5492..5755
                     /locus_tag="OQN12_003698"
     CDS             5492..5755
                     /locus_tag="OQN12_003698"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1655875.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            5745..6053
                     /locus_tag="OQN12_003699"
     CDS             5745..6053
                     /locus_tag="OQN12_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1655876.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(6095..6271)
                     /locus_tag="OQN12_003700"
     CDS             complement(6095..6271)
                     /locus_tag="OQN12_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCS1655877.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(6268..6678)
                     /locus_tag="OQN12_003701"
     CDS             complement(6268..6678)
                     /locus_tag="OQN12_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCS1655878.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(6675..6953)
                     /locus_tag="OQN12_003702"
     CDS             complement(6675..6953)
                     /locus_tag="OQN12_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655879.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(7028..>7250)
                     /locus_tag="OQN12_003703"
     CDS             complement(7028..>7250)
                     /locus_tag="OQN12_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655880.1"
                     /translation="SGGQIQCATSPASVLMLEKQRNGEGWEGGVPLFLDEQSHQFLQL
                     ESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1738 a         1960 c         1659 g         1893 t
ORIGIN      
        1 accaactgag ctaaagggcc ggaggcagaa taataaccat atgtcatcac atctgcaaac
       61 tcatctgacc accagcgtgt ttaacgtcct gtaccgtttt tcaggcataa aaaacccgcc
      121 cggaggcggg tttaagctgt gtggcgaagc aaccactctt aacagaatat cccgtttttt
      181 acgtacgtaa aatattttct agaaagtcgc cccttaccat cagggatgtt caatatattt
      241 gtccatttct aaccggacac ccaacatcat caacattcct tccaccaccc cctcggcttt
      301 ttgtagcttc tttcctatat aaccatctga gcatccatgc ttccgcgcca gcgtcatgaa
      361 tgtcatccca aacacgtagt agtcaaccag caagtcatgt agatcgttgt tgttccggtt
      421 aaggcgggcc atacacccac atataaccat cgcgtcatcg tcacagcatt gcgggcggga
      481 ttttactttt gaagggatta atcccttaaa accggcagca atggacgacc aggtcacatc
      541 ctcatgatta ttagccaccc acgcgcccca gcgctcaaga acctgctgaa tatcacgcat
      601 cactgtcttt acccctgtcc catccacgat gaacaatcag aacaccatca acaatggcgt
      661 gccctttgcc ctctttatct tcagcatatt ttcttaccgt ggcgcgatta cagttcagca
      721 ttcgtgaaac ttcggtcata ttacctcgtg tctggataag cagttccggt atcgtttgaa
      781 ttttgacgct catcaaatac tctccggttc ggtgattttt atcccgggct ttccaccagg
      841 gacataatga tcgcgcacaa gccagggcaa aacaaactca tggcgcatca gcgcagcgcc
      901 tcctgcacca gtttttcaaa ctttccgact ctggtttcca gctctgccac acagtccacc
      961 agctcatcta ctgctttctg tgcgcgatgc ttcgcctgca tcagttcccg aagcgcgggt
     1021 accatatctt tacggatagc atcttttgtt atgcccgttt tttcgagttg ttcagcatga
     1081 cgcagcattt cctgcgcgtg tttacgcaat tgttcagggg taaaagtcat tgtctggttg
     1141 ttcaaaagaa acgctccatc ttactgctgt cagttcgttt attactgtat ctgcgcggat
     1201 tgccgggctt catgggagtg gaaagcaccc gtgcactttc ctggtccaca ggcagaaaat
     1261 gtccgttata aaaacgccgg taaatcgttc ccagagaacc gttacgttgt ttcgtgatat
     1321 tgatttctgc gatgcccctg gcctgcgtat ccgggttgta cacttcatcc ctgtaaagca
     1381 tcagaatgat gtctgcatcc gcctctattt ctccggaatt tttcaggtct gagttcatgg
     1441 gacgtttatt gggtctggac tccacaccgc gggagagctg gctcagcgca atcaacggaa
     1501 aaccaccgga ttttgccagg cctttaagcc cctttgagat ttcacccacg gcaaggtcat
     1561 gacgccccgt ggttcgggtt tttatcagcc cgagataatc aaccaccacc agcgccgttt
     1621 ccggatattt aatcaggtgg tgtttcgttg ttgcgcatat ctcatcaatg gccaggttcg
     1681 cctggtccac catccagata ttgcgcccgg tcatccgccc caccccttgt gagaaacgcg
     1741 tccagtcttc atcttcaaag tgagccacag atttcaggcg tgatactggc atccctccag
     1801 ccgcagacac catacgttca ccaatctgga tgttcgccat ctccatggtg aacagaagca
     1861 caccatgccc ctgctcagtc accttgtcga tgatgtccag cgcaagttcg gttttcccca
     1921 tcaaaggacg ggcggcaatg aataccaggt ctccgggctc cataccgcct gtttttgcgt
     1981 ccagttcatc aataccggtc atcaacgtcc tggatttctc cagcccctga ttccggcatt
     2041 caacacgctc aaccacttcc ggaagcacat catcaatatg taccggctga atgacgccct
     2101 ttcctgtcga cagtgtgacc atcatgttct gcgcatcctt cagggcatct tcagctgctt
     2161 cacaggtatg cgcatcacgt aatttctgca gcgcctcatt cagtgttttt tctgcatcgc
     2221 gcagtgcggc attgcgccgc aacgctgcaa catagtgctc cagtgaagac ttcacccagg
     2281 ttttacgccc ggtatcagta atcaccgggg caagttccgg catctcatta cacaacagca
     2341 cggggtcaat cactcctgaa acacgggcct gtctgcagat gcctgtatag atatcctgat
     2401 acgctcgtac agaaaaaacg tccgctggca gtgtggccag aatatccatc acttcaggat
     2461 ctgctccgcg cagaaagaac gcgccgatga cagcgccttc aaggtcatcg ttacgccata
     2521 ctggagttgt catgcagcca cacctctgat acgagaacgg taactgggcc agttaaacga
     2581 caaccagttg cgtcccccgt ctgtgatcct gtcggcaatt cggggactga tgaacgccca
     2641 caactcttcc ggtgagaggt tactgataag aatggtgggc aagatacttt cgtacctggc
     2701 gttgataatt tcctgcaaaa tagccatttc agccgcgctg ccaaactgaa cgccaacttc
     2761 gtcgatgatc agcaaatcca ttgacgcata acgctcaata acttcatccg ctgttttttc
     2821 gctgtcattc cgccagcaat ttttcacagc acgggtaagg cgcatcacgt cggtgatctc
     2881 cacactggcc agatagttac ggatgatgtg ttttgccata gccacagcca gatgattttt
     2941 tccggtacca caactgccgg tcataacaag actggtaccg ttctccagca tatctggcca
     3001 gttctccgca tagcggcgac aggccgcaag atttctggct gcgccaggat taacctccag
     3061 ataattatca aactcgcagt cccgaaaacg cagggcaatt ccggcgttat cagtcagctc
     3121 ttccgccttg atggacgaca gctccatggt caaatcgttg gcctcagcga ttaagcagtc
     3181 agggcagcat gaaatttctt ctctgtcctc gccattacga tcgctccaca ccagtatatg
     3241 cgtgtgatat tcgccatgtt tttcgcaata tccgcgacct tcacgcatca ggcaggaacg
     3301 ataaggccat ggcttttcgc ccttctgagc aaatgcaatc tctgcccgta actcatccat
     3361 tcgcgcctgt agtctttttt gttgttcacg caggttaaac gtcatcatcg ctgtcacctc
     3421 agaatgtcag tttgtcactg gatttaccga atttgtcaga catggctccc aggccagcca
     3481 ggacatcgac ctgtcgctgt cgcccacctc tgggagcggc tggctgttgc cagaagtctt
     3541 cgaagtgacg atcgggtcca aagaacgtcg cagcctgctt cacgaactgt gtgccggtat
     3601 ttcctgtggc acgtacccag gcagcatagc gtttcacgcc atcaagcatg gcttcaggtt
     3661 ttattccctc cctgagccgg gctttccagg ctttgaaggc tgcggatttt gagttaccac
     3721 cagcacgttt tggatattcc tgccaggcct gttcaaattc cggtgaatat tcctgtcggg
     3781 aagaacgcgc tggtgcagac gcgtcagcgg atgcgccaat agtgttttta ctctctgtag
     3841 tattctctga agtaatctct gttgtattct ctgtaagatc gaaattggtt ttcccttctc
     3901 cgcggcgagg ggtttcccgt gtccgcggtg aaggctttcc ctcctccgcg aaattgggtt
     3961 ttacagtttc ccgaaaacgg gtttccccat ttcgggaaaa ctgattgttt tcattgataa
     4021 tttcattaag gcgctcacaa tctatacggt agaacatttt gtgctcaaga cgcttgttgg
     4081 tttcaaccaa aatgcctctg gacacaagat gcttacgcgc tacagcctgt tgttcaaatg
     4141 taagtccggt ttcgtgttgt atctcttcac gcgttttatg tacgccttcc gctgcatgtg
     4201 ctttatcctg ccagtaaaaa atctgaccaa agaaaataac agcgtgcgga cttcccatgt
     4261 atttaacgag cccagggtaa taagcaaccg gatgtccaaa atcgagcaga agatcagacg
     4321 gacgcatagc cacctcccag gcgtttaaac atttttccgg gctgaaacgc caccagcgga
     4381 taactcaggg tatgagtacg tccctgaacc tggcagacaa ccttctggct ttcggtactg
     4441 accaggcaaa cccgcagaac gtagccgttg ctggtggtga accactgccc cacacggggg
     4501 caatggttgt atcggtgata caggaaatta acgatgtggc ggatcatggg cgcacctcct
     4561 tgtcagaacc attcagcctg gaatcaacaa gtgcagcacc aaaaacagca tcacctacac
     4621 ggtcgtacag tttgccagcc agcggagatt caacggcctt aagcattggg taaagctggc
     4681 ttgtccagat ttgatggatt tcacgcaaat gcaggtatac gcctctggcg ttttgtgcga
     4741 cagatgacat gtcagccgca ccagctcctg ataagctcct ctccatctgg ttaaaggcat
     4801 tgatgtatgc ctctttgaac cgggcggcac gtttaccagt gaagcccata gcaaggaagg
     4861 cgaagccgcc gcgggtaatt tggtagcagg gaagtttgcg agtaccgccg ttgggctggc
     4921 gtaccaaaat tgatgtctcc gcaaaattgc gggcacaaaa ctctggagaa caatccaaaa
     4981 tgcggatctt tttcagaaca tcgtcatgac gtttagagaa gaagttggca acagccaggg
     5041 atgaagtaac agcctaacca tcaacgatgg caatttcagg ttgagagagg gttgggagag
     5101 tagtcatggt gacagcccct atgttgaatt caaagaactc accacatggg acgccaatca
     5161 cagaggtggt gagacgtaca gggttggcgt aaccggtcaa catagaaccc ggcgcatctt
     5221 gcgatgtccc tgcacgcccc accataattt gggcgtagca atgctcatga cacgaaaaaa
     5281 ccgcatgagc gcggttatgc tctatattga atttcaggac gccaatcccg gcccccgctt
     5341 tataaggtgc agagacagtg taacgtcccg aaattgcaga atcaatatat tgtgcagata
     5401 taacgatttt gttatgtttt gctgtatgaa ctacactatc gaatactaca gtgaagaggt
     5461 ccgactggag gtcgatcagc ttccattgag tatgcgtgcc agataccagc atcttgttga
     5521 acgcatgaag gtatacggca gcaatctcgg agaacctcac accagcgcct ttggtgacgg
     5581 gcttttcgaa ctcagaatta aaggcaacga tgggatcgcg cgtgtttttt actgcactct
     5641 gacagggaaa cgcatcatca tgctgcatag ttttgtaaag aaagcgcaga aaacaccgcc
     5701 agccgaacgc aagaaagctg aaaccagaat gaaggaggtt aagcatgact ggtaaacgca
     5761 ctctccccac catgacacac gacgaaatgg cagccaaatg gatggaagac ccggctttta
     5821 aagcagaata cgacgctatc gctgacgaat tcgcactgct tgatgaaatg ctggcagcac
     5881 gcaaagaagc tggcttaact caggctgaag ttgccgagcg aatgggaaca aaagcgaccg
     5941 cgatcaccag aatggaaagt aatctcgcat caggtatcag cggcccatca tttgccacac
     6001 taaaaaaatt cgcccgcgct actggaaaaa aactccagat ccgcttcgtt taaccacacc
     6061 gcgccgtcat tctggcggcg caccggataa atagtcattt cctcgcacga tgtcttagcc
     6121 accggatatc ccacaggtga gtcgtgtagt tgaaggtttt tacgtcagat tcttttggga
     6181 ttggcttgcg tttatttctg gagcgtttcg ttggaaggta tttgcagttt tcgcagatga
     6241 tgtcggtgaa acttcgtcga tgtcgcctca ttcatacctc ctgtcggtaa atctgacacc
     6301 ctgaccaata gcccaggctg ttgtgtactc gatcagactt gccatgcgct tcacgctcat
     6361 ctgcgcgctg ctttcgcgaa tgttgacgca ttctccttca aggccgggca aaacatcagc
     6421 ttcctgtttt gttgccactg catgaccgct gatcaacaaa accttccatt gttctggttt
     6481 taaccatttg ccgcaccatt gaacctgacg agcgatatcc gccagcatcg cgtgaaattt
     6541 tgcgttctgg tcaagatttc gcttgtagtc agtaatgcgg atggtgactg gcttgtcttt
     6601 atcgagagga gttgagagga tggcatttat tgcggcttgc tgttgttgct tagttcggag
     6661 gaagattgtt tgcttcatcg aaaattcttc tctttaattc cagcggctct gatagctttc
     6721 attactgcaa ttaccgtttt gtccttccca tcctcatgcc ccatcgcata agcaccttct
     6781 tcaccatctt tccaaaagtc gtcattcgat tcgggccagt cgatatccag ttcaatagct
     6841 gctcgcgatg cctgccatat cacccaggca aactctttta attcatcgtc tcctgtgaac
     6901 tggcttttgt cttttgacca ccagttttca aactgtcggt agctatcgtt cacttccctc
     6961 tcccccaaat aaaaaggcct gcgattacca gcaggcctgc tattagctca gtgatgtaga
     7021 tggtcattta atactccgtc acgttttcct gtcgccacgc ctcgtcatat tccgatttcg
     7081 gcatattggc gatgtagcta tatggcgatc ctgattcaag ttgcaggaac tggtgcgatt
     7141 gctcgtcaag gaacaacgga acaccacctt cccaaccttc gccgttacgt tgtttttcaa
     7201 gcatcaaaac agatgccggt gaagtggcac actgaatttg gccacctgaa
//
LOCUS       DAJVRP010000148         6857 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVRP010000148 DAJVRP010000000
VERSION     DAJVRP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.148"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(271..888)
                     /gene="tdk"
                     /locus_tag="OQN12_003704"
     CDS             complement(271..888)
                     /gene="tdk"
                     /locus_tag="OQN12_003704"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1655881.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1493..1906
                     /gene="hns"
                     /locus_tag="OQN12_003705"
     CDS             1493..1906
                     /gene="hns"
                     /locus_tag="OQN12_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1655882.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2050..2958)
                     /gene="galU"
                     /locus_tag="OQN12_003706"
     CDS             complement(2050..2958)
                     /gene="galU"
                     /locus_tag="OQN12_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1655883.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3160..4173)
                     /gene="rssB"
                     /locus_tag="OQN12_003707"
     CDS             complement(3160..4173)
                     /gene="rssB"
                     /locus_tag="OQN12_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1655884.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4265..5170)
                     /gene="rssA"
                     /locus_tag="OQN12_003708"
     CDS             complement(4265..5170)
                     /gene="rssA"
                     /locus_tag="OQN12_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1655885.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5283..5741
                     /locus_tag="OQN12_003709"
     CDS             5283..5741
                     /locus_tag="OQN12_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1655886.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5791..6633
                     /gene="purU"
                     /locus_tag="OQN12_003710"
     CDS             5791..6633
                     /gene="purU"
                     /locus_tag="OQN12_003710"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1655887.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         1866 a         1675 c         1499 g         1817 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaactttt ccataaaccg acagcattag
       61 ccttcatcat atttgcgacg atgtataacg cctaaacaca gggatattgt actttacagg
      121 tcacaagtca acgtcggtgc ttaagagccc tgtgaggcgt atagcggcgt taaaaaactg
      181 ccgagaaggg tatatagccc ggaagaagtg cgtaaaacga actgacagga taaaagtgcc
      241 ctgctcaccc tgtcagtaaa gaaattctta ttaatcgtgg cgatgccttt cctgaatagc
      301 cgttaatgag ccgacttgta acgcctcttt atagtgttta cggcatacag aaacgtatcg
      361 ttcattacca ccaattacca cctgctcacc ttcgttataa ggtctgcctg cttgatcaag
      421 acgcagcacc atgcttgctt tacggccaca aaaacagatg gtttttaatt caaccagttt
      481 gtcggaccat gccagtaagt attggctgcc aataaataat tcacctcgaa aatcggtacg
      541 taaaccataa caaagtacgg gtatatcgag ttgatcgaca acctccgata attcatatac
      601 ttgttgtctg gttaaaaact ggcattcatc aaccagtacg caatgaattg cctgctgttc
      661 atgttccgca cgaatctcat caaataatga tgaattttgg ttaaataatt ttgcaggcga
      721 tgacaaacct atacgcgaac tgactttccc ggcaccaaag cgatcatcaa tttctgccgt
      781 atatacgaca gtgcgcatgc cgcgttcctg gtaattgtat gaagattgca acaatgctgt
      841 agacttaccc gcattcattg cggaatagta gaaatatagc tgtgccatca gccacaggcc
      901 ctcaatgata tgaaataaag ttggctggag tttatcataa ttcgtcagtt tttcagctca
      961 acagtatgca cagaagtggg tatatgcgtt ctcccttacg aagccttgca taatccttct
     1021 gagctatcat tacaactgcc tcgcttgtta taagcgggta aatgactgct ggtaactatt
     1081 cacaatcttt aacctgttgc gcaagtaata gccctctgtt gacctccagg agatagtgca
     1141 atactaagtc catgctctta ttgcgactgt tctacttttc atcattcgct taatagggaa
     1201 ttctcgtaaa cacaactaat acagaagact gaaaggtcgt cagcctacga taatctcccc
     1261 ataaaatgtg acatgaatca ggaagtttta acctcacgtg ctgcgaaatc atcggtgtaa
     1321 atagggctat atgccgcgtc ttttctggct aattttatga aaagatattt attggcggca
     1381 caaaataaag aacaattttg aattccttac attcctggct attgcacaac tgaatttaag
     1441 gctctattat tacctcaaca aaccacccca atataagttt gagattacta caatgagcga
     1501 agcacttaaa attctgaaca acatccgtac tcttcgtgcg caggcaagag aatgtacact
     1561 tgaaacgctg gaagaaatgc tggaaaaatt agaagttgtt gttaacgaac gtcgcgaaga
     1621 agaaagcgcg gctgctgctg aagttgaaga gcgcactcgt aagctgcagc aatatcgcga
     1681 aatgctgatc gctgacggta ttgacccgaa cgaactgctg aatagccttg ctgccgttaa
     1741 atctggcacc aaagctaaac gtgctcagcg tccggcaaaa tatagctacg ttgacgaaaa
     1801 cggcgaaact aaaacctgga ctggccaagg ccgtactcca gctgtaatca aaaaagcaat
     1861 ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag caataatctt ttgtagattg
     1921 cacttgctta aaatcccgcc agcggcggga ttttttattg tccggtttaa gacaatttaa
     1981 taagaataag ctataaaaaa acggcgtcga ttgctcaacg ccgtttcgtg gataacaccg
     2041 atacggatgt tacttcttaa tgcccatctc ttcttcaagc caggctttaa attccgtgcc
     2101 aagggtgtta tgacgaatac cgtattcaac gaaggcctgc atgtaaccta atttattacc
     2161 gcagtcatgg ctcttccctt tcatatgata ggcttccacc gtttcttttt cgatcagcat
     2221 atcaattgcg tcggtgagct gaatttcatc accagctccc ggaggggttt ttgccagcaa
     2281 cggccaaata tccgcgctaa gtacgtaacg acccacaata gcgagattag acggcgcaac
     2341 atccgctttc ggtttttcta ccacaccaac catcggtacg ctttcacccg gcgctaattc
     2401 aacgcctttg caatccacaa cgccatatgc ggtcacatca gcaaccggtt caaccatgat
     2461 ctggctatga cccgtttcat caaagcggcg gatcatctct gccaggttat cctgtgacaa
     2521 atcggattca tattcatcca gaataacatc aggcaaaata acagctaccg gttcatcacc
     2581 cactaccggg tgagcacaca ataccgcgtg tcccaggcct ttcgccagac cctgacgaac
     2641 ttgcataata gtcacgtgcg gtggacaaat agactgcact tcatcaagca gttgacgttt
     2701 tacacgtttt tccagcattg cttccagttc aaaactggta tcaaagtggt tttcaataga
     2761 gtttttagat gagtgtgtaa ccagcacaat ttcagtaatg ccagccgcaa tacattcatt
     2821 cacgacgtat tgaattaatg gcttatcgac aagtggcagc atctctttcg ggatggcttt
     2881 cgtcgccggc aacatcctgg ttcctaatcc cgcaacgggg ataacggctt ttttgacttt
     2941 cgtattaatg gcagccattt aaattctcct ggactgttcg tgttttgaac gtgttcatat
     3001 ttctgtatcg catcccagta tatcagcacc ccgaaggggc taagttctga aaaggaggcg
     3061 agatagcata attaagacaa ttacttatac gaatggcgac gatagtacca ccagccgaca
     3121 ttagcaggta atgcaaattt agcccgcgtt atcgtttgct cattctgcag acaacatcaa
     3181 gcgcagtcga ccaccggttc cccatatttg gcattgccag gcatcgcatc gctggctcaa
     3241 ttgattcaaa taagcgttac ctaaagtgcc taacggaaca ccattactga tttgcacctg
     3301 gtgttcgccg gtatttaacg tcgcattcag acccgcagaa accagaatga gatttttcag
     3361 ttcgcgatga taatagccaa ctaatagcgg aaactgcccc ggcagattgg cctgacgaag
     3421 taaatggttt acctgcttca ataacgcgcc caactctggc aaccgttgat tttggtgtgc
     3481 aagctgttcc tgtaataatc cgttaaacaa tgcgcgtaat aacaaggcag caagtacgcc
     3541 attatgtcca gctcgggtga catcaaggca ataaaatgcc agatcgtttt ccgaaagtgc
     3601 ggcaatatca agcaccaggc cgggtttgtc cgcggcaacc aattgacggt aattaacccg
     3661 gcaatgggaa atcacctgct gaaccggcgg ttgtagttcc tgtaataatt tcgccgctgc
     3721 ggcagggtta tcaaccattg catcccagtc gcgaaaaagc ctttcctctt cctcaacgcg
     3781 cgaattaaac atgctgggat agagacaggc aaaaaccatc tcgcgcaagc gattcagatc
     3841 tttaactggt ttcagcaaaa catcttcaac gcccagacgt aacgctttgg caatatctgc
     3901 catattttca gtggcagata tcaccagaac tggggtctgg tcgcctctgt tacgtatatg
     3961 ctccagcagt ttaagcccgt tcattcgtgg catcgcgata tcacatatca tcaggtctgg
     4021 agtgaaacct cccagcaact caagggcatc caccccatca gccgccagta ccgttgtcgc
     4081 tcccaatgag gaaaaccatg aatccagaag cgagcgaaat acctgctcat cttcaacaat
     4141 gagaatctgt tttccgacca atggctgcgt catgttctct cccctgactg gctttactca
     4201 atagtggcat gctattgcca cctgcgcctg tcagaattaa cttaagtgta gataaaaatt
     4261 ctggtcaaat gttggtgcgt accaacggca aaagttcgtc cattttcctt tccactgcca
     4321 gctgtccggc cgcaatggca gcgtgcgcgc gatggaaatc aagcgtagat atttgcgggc
     4381 aaacaggttg aatcagaata tcgggcggat cacctgccat gcggttcctt ttaaggcggt
     4441 tctccaacac ctggatagac gtggtcataa tctctgttgc cgttggcgct gtcacagccc
     4501 gacgtgtcgt tatgctgcct aacctttctt tcagacgcgc atgccacggt agagaatcac
     4561 cattctcgct attttcttca ctgacattaa aggagagcag atcttgttgc atcaaatgag
     4621 catcgtgctg caggtcgacc gctatcacaa tatcagcccc caatgcacgc gtaagagaaa
     4681 taggaattgg gttaactact gccccatcaa ccagccagta gccgttatgt gcaacgggtg
     4741 ccatgagtcc tggaatactg catgatgcgc gaatagcaag atggagatcg ccttcagtaa
     4801 accataattc acgccccgta cttaaattgg tggcaacagc cgcaaagcga cgggaacaat
     4861 tttcgatctc tgtttccggc attatttcgc gatattgatt gaagacacgc tcgccgcgca
     4921 gtaacccacc gcgctgccag gagaggtcca tcaggcgtaa aacgtcccaa taactgaaag
     4981 aggttaccca atcttccagc gcagataatc gatcgcatgc ataggccgcg cccaccagcg
     5041 aaccaattga acatcctgca acgatatcaa tttcaatacc cacttttttt agcgcattaa
     5101 taacgccaat atgcgaccaa cctctcgccg cgccagatcc cagcgccagc cctattttta
     5161 tctttctcat tattcccgca agattcccct ggaatgcaat cgtagccaca tcgctactgc
     5221 tcggttaaca tagtgctact caggcggtta atacgccgtt attgtttcca gggagattat
     5281 ttgtgtctca gctttgtccc tgtggtagtg ctgtcgagta tagcctatgt tgccaccctt
     5341 atgtgtctgg tgaaaaggtt gcacctgatc cagaacatct catgcgttcg cgttactgcg
     5401 cttttgtgat gcaagacgct gattatttaa taaagacctg gcatccctct tgtggagcag
     5461 cggcgttacg tgccgaattg atgaccggat ttgcgcatac tgaatggttg ggattgaccg
     5521 tatttgaaca ttgttggcag gacgccgaca atatcggatt tgtcagtttt gttgctcgat
     5581 ttaccgaagg tggcaaaaca ggtgcaataa ttgaacgttc gcgatttttg aaagaaaacg
     5641 gtcaatggta ctatattgac ggtacacgtc cgcagtttgg tcgcaacgat ccctgccctt
     5701 gtggttcagg taaaaaattt aaaaagtgct gcggccaata atggttgacg gtacggttta
     5761 gcaaacactc tcaacaaggt ttttccagca atgcattcac tccaacgtaa agttctgcgt
     5821 actatttgtc cggaccaaaa aggtctgatc gcacgtatta ccaatatttg ctacaagcac
     5881 gagttaaata tcgtacagaa caatgaattt gttgatcacc gtaccgggcg cttttttatg
     5941 cgcacggaac tggaagggat ttttaatgat tccaccctgc tggcggatct cgatagcgca
     6001 ttgccagaag gctccgtgcg tgagctgaat cctgccggtc gtcgccggat agtgattctg
     6061 gtcactaaag aagcgcattg ccttggcgat ttgttgatga aagccaatta cggcggtctg
     6121 gatgtcgaaa tcgcggcagt tattggtaac cacgatactt tacgttctct ggttgagcgt
     6181 tttgatattc cgtttgagct ggtaagccat gaagggttaa gccgcaacga gcacgatcaa
     6241 aagatggcgg acgccattga tgcttatcaa cctgactatg tggtgctggc gaagtatatg
     6301 cgggtattaa cgccggaatt tgtggcacgc ttcccgaata aaatcatcaa tattcaccat
     6361 tcattcctgc ctgcgtttat cggcgcacgt ccttatcacc aggcctatga acgtggtgtg
     6421 aagattattg gcgcaaccgc tcactatgtg aatgacaatc tggacgaagg cccaatcatc
     6481 atgcaggacg ttattcatgt cgatcatacc tacacagctg aagatatgat gcgcgcaggt
     6541 cgtgacgtcg agaaaaacgt cttaagtcgt gcgctataca aagtactggc gcaacgcgtc
     6601 tttgtttacg gtaatcggac gattattctt taatcgccag caaaaataac tggttacctt
     6661 taatccgtta cggatgaaaa ttacgcaacc agttcatttt tctcaacgta acactttaca
     6721 gcggcacgtc atttgatatg atgcgccccg cttcccgata agggagcagg ctagtaaaaa
     6781 gcattacccc gtggtggggt tcccgagcgg ccaaagggag cagactgtaa atctgccgtc
     6841 atcgacttcg aaggttc
//
LOCUS       DAJVRP010000149         6798 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVRP010000149 DAJVRP010000000
VERSION     DAJVRP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.149"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(94..1721)
                     /gene="yeeO"
                     /locus_tag="OQN12_003711"
                     /pseudo
     CDS             complement(94..1721)
                     /gene="yeeO"
                     /locus_tag="OQN12_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1726..1801
                     /locus_tag="OQN12_003712"
     tRNA            1726..1801
                     /locus_tag="OQN12_003712"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1759..1761,aa:Asn,seq:gtt)
     gene            complement(1839..2789)
                     /gene="cbl"
                     /locus_tag="OQN12_003713"
     CDS             complement(1839..2789)
                     /gene="cbl"
                     /locus_tag="OQN12_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCS1655888.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2891..3808)
                     /gene="nac"
                     /locus_tag="OQN12_003714"
     CDS             complement(2891..3808)
                     /gene="nac"
                     /locus_tag="OQN12_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCS1655889.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4136..4211
                     /locus_tag="OQN12_003715"
     tRNA            4136..4211
                     /locus_tag="OQN12_003715"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4169..4171,aa:Asn,seq:gtt)
     gene            complement(4267..5202)
                     /gene="ldtA"
                     /locus_tag="OQN12_003716"
     CDS             complement(4267..5202)
                     /gene="ldtA"
                     /locus_tag="OQN12_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1655890.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5264..6343)
                     /gene="cobT"
                     /locus_tag="OQN12_003717"
     CDS             complement(5264..6343)
                     /gene="cobT"
                     /locus_tag="OQN12_003717"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1655891.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6355..>6798)
                     /gene="cobS"
                     /locus_tag="OQN12_003718"
     CDS             complement(6355..>6798)
                     /gene="cobS"
                     /locus_tag="OQN12_003718"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1655892.1"
                     /translation="LEIMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAA
                     ACAVSRGTAALLMYRHRYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGV
                     AAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGELVFLLALL"
BASE COUNT         1872 a         1826 c         1476 g         1624 t
ORIGIN      
        1 gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgtttt ttaaatttga
       61 aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg gctttttttc
      121 acacttttgc ggctcggatc gagggtattt ccacagccaa cgtccagtaa ccattcgcca
      181 gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga caccaaccac
      241 accccagcca agcatgattc cctgcacata accgactaca acccggcaac cccacatgct
      301 caacatagaa acccacatgg cgtaacgggc atcacgagca cccttaaatc cagcgggtag
      361 cacccatgag gcggaccaaa taggcataaa taaagcattt agctaaatca gaatcacaac
      421 gacgtgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg caaagggagc
      481 cgttagccag gcgatggccg ttaatccaag agtggacagc cagaacacat gccgcaactg
      541 aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga tcgtagaagc
      601 agaaccgagc gcacttccgg gtaagttgat aagagccgca attgagaacg cgataaaatt
      661 tccggcaata acgctggtcc ccatcccggc aacgaacatt tgggttaata accgaccact
      721 ggtaaataac actgattcga cactcgctgg aataccaatc cccatgactt cccagataat
      781 gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag gattaaaacc
      841 aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg ttaaacccaa
      901 cccagccccg accaggagaa aaggccgtaa atcaatatgc cgctaataat aatattaaga
      961 atattcaggc taccgttaat cagtagcggt attttcgtat tccctgcacc acgaagtgcc
     1021 ccgctaccaa taagagtgat ggcagcggct ggataactga gtacggtcag ctccagataa
     1081 gtcaacgcca gtgctttaac ttctgtcgtg gcatcacccg cgacgaaatc aataatttgt
     1141 tcgccaaaat gatgaattag cgttgccaac agtacggcaa acaacgtcat gatcaccaat
     1201 gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct taccgagact aaatgctaca
     1261 acgacagtag taccaagatc gatagctgca aaaaaagcca taatgaccat attgaagctg
     1321 tccgccaacc ccacgccggc catcgcatct tttcccagcc aactgaccag aaaagtgctc
     1381 aggcccccca tcaacaggac acaggcgttc tccatgaaga taggaacagc aagcggggtt
     1441 atctcgcgct agaacaacac tttgtagctc ttgcgtttag agtgccagcg agtgccgtga
     1501 acaacctggc gtaaagcaga ggagatattc aaagccgacc ttaattgcag aaagtgaaac
     1561 cacatttcaa atgatgaggg agaatcagca aagctgcaaa gattttcgcc aacaaattgt
     1621 ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt tgggaaattt aaaaatcggg
     1681 tttgacaaaa tatttttcgc cgttaagatg tgcctcaaca gcgattcctc tgtagttcag
     1741 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     1801 aaatttctga aaagcccgct tttatagcgg gctttttgct atatctgata atcaatttcc
     1861 tcttcactgt tttccatcac ctggcgcttg atatcctcaa ctgacagtcc tgcattacaa
     1921 aattccagaa agcgccaaac atagttacgc tgaagttgtc ctcgcttcag tcccaaccag
     1981 acagtattag catcgaaaag atgccgcgta tccaggcgga ttaaattctc ttcctcttgt
     2041 tcgccgctgg attgttcggc aactaatccg atcccaagcc caagagcaac ataggtttta
     2101 atgacatcag aatcctgtgc acttaataca atatctgccg gcaaaccttt gcgggtaaat
     2161 gcgtcatcag tgcgtgagcg ccccgtaatt ccctgtcggt aagtaattaa cggccacttc
     2221 gctattgatt ccagcgtcaa tggtgtaatt tgcgttaagg gatgatcgag tggaacaagc
     2281 aaactatggt gccaacaaaa ccacgggaag gcgacgagct gcgggtcatt actcaaacgc
     2341 tcgctggcga taccaatatc agcttcgcca ttttgcaaca acgtcgcaat ttcctgtggc
     2401 gaaccctgga ttagctcgag ccgaacctcc gggaaaagtt cgcgaaaagc tttaatgacc
     2461 tctggcaagc tataacgtgc ctgagtatgc gtcgttgcaa tagtgagaac accagacgta
     2521 tcgttggtaa acaggtctgc aagccgacga acattactgg cttcattcag aatacgttct
     2581 gcaatgacca gtaatgcttt gcccggttca gtcatcccca gcagtcgctt acctcgtcga
     2641 acaaatattt cgatgccaaa ttcatcctcc agttcccgaa tatgacggct aacgcctgac
     2701 tgtgaggtaa aaagcatatt cgcaacctct gtcaggttgt aatcctgacg tgcagcctcg
     2761 cggattatct ttagttgttg gaaattcacg gtaaactccg ggcagttcag atatcccgtt
     2821 attgttaaag tctaatgccc ggcataacaa ataataaaaa cccgcatctt attccatccc
     2881 gatataacgc ttagctcacc aattgccact gccttttttc catcactgga gaactaatca
     2941 ctgacattaa caactctttc actgcctgtg cctgtggtga caagtttgct ctggcgggta
     3001 aatttaagga caaagagaga ctcattgaag gagtggtaat gcgtgacatc cacccattta
     3061 ctgcgccaca taacgaacgc gcagccgatt caggtaatac tgcaacgccc atgccgctgg
     3121 caatcgctgc ggtaagcgtg gcaatagact caatttcacc aatgactttt gccgtgagcc
     3181 gccgtaggga aaaagcctca tcaacacgaa gtctaacagc gctgtaatca ctggggagaa
     3241 aaaggttcat ttgcgcaata gcattaacat caacgttttg ccccgggcaa tcttgagttc
     3301 ctaccaggaa aagatcttct ttcagcaaag cctgactgga taccccagcc acaggggaat
     3361 gctcataaat caccgccata tcgagttggt gatttatcag tttttcgtta agcactgcac
     3421 cactattttc atgaagatag ataacgacct ccgggaattc agcgcgaacc gcctgtaata
     3481 agggcatggt gatggatgac gcagcggttc ctggcgcaaa gccaatcgag acttgccccg
     3541 ataatgactg accaacgtta tgcaccgcca gttgggcctg ctcacactga cgtaaaatgg
     3601 cccgtgcatg ggtatagata atttttccgg cgtctgttgg cgtaacgccc cgttttgtgc
     3661 gaatcaaaag ctgttgattt aactcacctt ccagtatggc aacctgctgg ctgagcgctg
     3721 gttgtgcgat atgcaatact tcagcagcct gggtcaggct accaatatct acaattttta
     3781 cgaagtattt caggcgtctg aagttcatgt tgcctccggt ttttaagaat cggcccaagt
     3841 gccgccatta cttacaacca gtatgcaaga tgcctgccag ttctattttg gcgttgatgc
     3901 gcaagctaac caactgtcaa ataagagatt atgatagatt cgtcattggc cctttttatc
     3961 agcagtcgca ttcacctgcc ctataaaagg agggtatgca ccacgatggt tcattaccca
     4021 ataagattga aagctcacca ctttgttgaa attggcagca aacaaacaaa aaaaatgcat
     4081 ttcacccttt gacatcacca tgcactgcca ttaatatgcg ccccgttcac acgattcctc
     4141 tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt
     4201 cagaggagcc aaattcaaaa aagcctgctt tcgagcaggc tttttacttt ctaattacca
     4261 acgctcttaa aacatctgtc ttgaaccaga actaatttgc acaggcattc ccgatcgatg
     4321 ttgcaacgca gcatttgcgc gatttacatc aacttcttgc ccgttgataa acgcccgcaa
     4381 agatggggtt accggcaatg gcacttttcg gtcagactca tattctgcac gattgcgcga
     4441 cagcggctca tgaacttcca gccactttga accatccggt tccgttgtgt atttcactgg
     4501 ctgatcaata atttgtacac gagtgcctac aggaacatta tcaaacagat atttgatatc
     4561 gtcattacgc agacgaatac agccctgact tacacggagc ccaataccaa aattggcatt
     4621 ggtaccgtgg atggcataca acctgccaat ataaatcgcg tacagcccca tggggttatc
     4681 gggccccgca ggaacaaatg cgggcaaact ctcccctcgt ttcgcatatt cgcgccgggt
     4741 gttcggcgtt ggcgtccagg ttggtgcttc ttgtttacgt tcaacggtag tcacccagtt
     4801 acgcggggtt tctcgcccag cctggccgat accaatagga aagacttcca ctgtattact
     4861 gtctggcgga tagtaataaa gacgcatttc agcgacgtta accacaatcc ctttacgaac
     4921 agtggcgggc aaaatcagtt gctgcggaat ggtgagttgt gagccaggct tcggcaaaaa
     4981 aacatcagcg cccggattcg cttccagcat attacttaac ccttgcccgt attgtgcggc
     5041 aaaagtctcc agcggctgag tattgtgatc aggaacagtt acagtaagcg actgccccac
     5101 taaacggcta ccctctggag gtaatggata agttaccgcc aggctatttt ggctggcaaa
     5161 aagcagagca aatgagcaaa gaatatttac acgacgcatc atgtcccttt cctaagtcgc
     5221 gaatactatc cgataagtat agcttttatc agacttttcg tttttagctg ttcaaatcag
     5281 aagtcgtatt ccccggtaga acaatattac tggcagcaag ttcgcccatg ttgttgtata
     5341 tcgcacaagc agcttcgatg atggacatcg ccagagctgc accactcccc tcacctaaac
     5401 gcatatccat attgagataa ggctccagcc ccaaatgcga gagcgctata cgcgcgcctt
     5461 tttctgccga caagtgagaa ggaatgagat acggtttgat tgcaggagac atctggcagg
     5521 ctgcgagcgc agcagcataa gaaagaaatc catccagcaa cacgggtaaa ccgcaggaag
     5581 cagcacctaa catcactcca gctattccga ccaaatcaaa tccacccact tttgccagga
     5641 catcaacacc atcctgagga tttggttgat tcaacgtaat cgcccgacgc acaacatcaa
     5701 ttttattagc cagtttatct gtcggcaggt ttgcgccaat cccaaccact tcttcaggat
     5761 cccggccagt gattgtgctg actattgccg ctgccggtgt cgtgtttgcc atccccagtt
     5821 cacctacacc aaacagcgtg acaccgtttt tcgccagctc ccgcgtataa catatgacgt
     5881 ccaaaagcaa cttttcagcc tgacggcgac tcattgccgg agctgaagca atattgccgc
     5941 tacctcgtgc gacacgcatg ttgataagcc cggggatagg ctcagcagta tcaataccaa
     6001 catcaattac gtggacgtta gcgcccgctt gtgctgccag cacacacacg ccggttgttc
     6061 cacgggtcat attttcagcc tgtatggctg tcacttcttt tggagaaata gcgacccctt
     6121 cctcccagac gccgtgatcg gcacacataa ccagtaccgc ttttttgccc acatgcggta
     6181 tgccattcac ccccggcatt cctgccagtt gtatggcaag cgcctccagc tttcctaggc
     6241 taccaacagg tttgaataac ccgtcaacat gccgttgtgc acgcgacata gcggcagaat
     6301 tgatggctgg gatcgtattc agtaaatcgg caattatttg catgtcacgt ttctttatag
     6361 cagtgccagc aggaagacca gttcaccaag ctctatggct gctcccagcg tatcgcccgt
     6421 ttgcccgccc agcgtacgtt tgagaagctg accgaggatg aaaatcgcca ccatcgtcac
     6481 caccatagca gccacaccat gcatacccag caataatacg gcagcaaaaa tagcggctaa
     6541 gccgagtgtg acgcaggttt gtcgcccatc aattttgcca atgaacacat tgccaagccc
     6601 ttcttcccgc gcataacgat gacgatacat caataatgcg gcagtcccgc ggctgaccgc
     6661 acatgctgcc gccagcgatg caaggatcgg ttcgccacgc agagccaact cgcttaacac
     6721 cagaatcttt gccagtacca caaaaatcaa tgccagaccg ccgtgggtgc ctaaacggct
     6781 atcacgcatg atttccaa
//
LOCUS       DAJVRP010000150         6749 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVRP010000150 DAJVRP010000000
VERSION     DAJVRP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.150"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            42..1466
                     /gene="sbcB"
                     /locus_tag="OQN12_003719"
     CDS             42..1466
                     /gene="sbcB"
                     /locus_tag="OQN12_003719"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1655893.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1509..1736)
                     /locus_tag="OQN12_003720"
     CDS             complement(1509..1736)
                     /locus_tag="OQN12_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1655894.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1750..2808)
                     /gene="yeeE"
                     /locus_tag="OQN12_003721"
     CDS             complement(1750..2808)
                     /gene="yeeE"
                     /locus_tag="OQN12_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS1655895.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(2987..4345)
                     /gene="plaP"
                     /locus_tag="OQN12_003722"
     CDS             complement(2987..4345)
                     /gene="plaP"
                     /locus_tag="OQN12_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1655896.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4335..4397)
                     /gene="yoeI"
                     /locus_tag="OQN12_003723"
     CDS             complement(4335..4397)
                     /gene="yoeI"
                     /locus_tag="OQN12_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1655897.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4558..5541)
                     /locus_tag="OQN12_003724"
     CDS             complement(4558..5541)
                     /locus_tag="OQN12_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1655898.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5587..6411)
                     /locus_tag="OQN12_003725"
     CDS             complement(5587..6411)
                     /locus_tag="OQN12_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1655899.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6494..6748)
                     /gene="yoeB"
                     /locus_tag="OQN12_003726"
     CDS             complement(6494..6748)
                     /gene="yoeB"
                     /locus_tag="OQN12_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCS1655900.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1823 a         1774 c         1582 g         1570 t
ORIGIN      
        1 ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac acagataaac
       61 aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg ttagatcgcc
      121 ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc gaacccgaag
      181 tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta ttaattaccg
      241 gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt gccgcccgta
      301 ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat gtgcgtttcg
      361 acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac gcctggagct
      421 ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt tatgccctgc
      481 gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt cgccttgagc
      541 atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg gctgatgtgt
      601 acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg tttgattatc
      661 tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg cagatgaaac
      721 ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc tgggtggcac
      781 cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg gcaggagaca
      841 tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat accgcaaaag
      901 ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat aaatgtccgg
      961 tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga attaatcgtc
     1021 agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg
     1081 tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat gcacagcttt
     1141 ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg gaaaccgagc
     1201 cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa aagttgttgt
     1261 tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag caacgctggc
     1321 tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct gatgaattgc
     1381 agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta aaagcacttt
     1441 ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg gcggaggaca
     1501 atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat ctgctgataa
     1561 tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg
     1621 caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc tgcttttgct
     1681 tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat cgccatcttt
     1741 acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata
     1801 cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa tccagccctg
     1861 ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac ctgcaatact
     1921 ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt gtgcatcagc
     1981 tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca cgaagatccc
     2041 taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca gaaattgaag
     2101 aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg cttcactcag
     2161 gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg gatgccagcg
     2221 tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag
     2281 ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca aaacggtaac
     2341 caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag tagtgtagtg
     2401 ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac tcatcaccat
     2461 ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat accaggtccc
     2521 ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac ctataacagt
     2581 accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa
     2641 aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc gattattttt
     2701 cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct gcatgacaaa
     2761 tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa attaattaac
     2821 cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca ataaccaaat
     2881 tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa taaaaaaacc
     2941 ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat gctacgtctt
     3001 cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa atcagaccga
     3061 tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac
     3121 caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct
     3181 tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca aaggtgaacg
     3241 ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag ttgattgcca
     3301 gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat ttcgggtgta
     3361 cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac atcagacgcg
     3421 ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg
     3481 cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg
     3541 gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga
     3601 agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg cgctctgcat
     3661 ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga
     3721 ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac ggacgcgtgc
     3781 tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca
     3841 ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct acggatttca
     3901 gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg
     3961 gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg
     4021 cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg
     4081 atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc agcttcccgt
     4141 agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac
     4201 catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc tgcatatagg
     4261 ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg
     4321 tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac cgtcgcgtaa
     4381 gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga tttttagtaa
     4441 atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga agcaaaaaat
     4501 aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc attgtgccta
     4561 aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc tttgaatggc
     4621 tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg ctaaaatttc
     4681 atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa tccagacagg
     4741 ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat cgagaaaacg
     4801 ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca gctccagttt
     4861 ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg aggccgtaga
     4921 agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa tgatacgacg
     4981 gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa agacttgttc
     5041 aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat gcatcgcgcc
     5101 aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga gtacgccatt
     5161 gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga gaggcgcaag
     5221 cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc agccttcatg
     5281 cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc gccctttctc
     5341 taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat caagcaattg
     5401 aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta
     5461 gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga ggatcatcaa
     5521 cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc ctgctggcgt
     5581 gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa atcccagttc
     5641 attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac tatttctgaa
     5701 ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat tacgcgcagg
     5761 gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta acaacagagt
     5821 gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac catcaggggc
     5881 ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc ccgcaagacg
     5941 cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt cttctaatac
     6001 tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc cttgcgcgtc
     6061 gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg ccagcgcgct
     6121 atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc tacgacgtgc
     6181 cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt cagaatcgca
     6241 aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc gggccgcttc
     6301 gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg aaagtgacat
     6361 cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca tccgttatct
     6421 cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca attcattcta
     6481 tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa tcatcagtaa
     6541 ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct gacaaattat
     6601 gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta tctttgataa
     6661 gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga taatcatccc
     6721 atgattcctc agaccagatt agtttcact
//
LOCUS       DAJVRP010000151         6617 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVRP010000151 DAJVRP010000000
VERSION     DAJVRP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.151"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            62..2494
                     /locus_tag="OQN12_003727"
     CDS             62..2494
                     /locus_tag="OQN12_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCS1655901.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2561..4030
                     /locus_tag="OQN12_003728"
     CDS             2561..4030
                     /locus_tag="OQN12_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1655902.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4030..5691
                     /locus_tag="OQN12_003729"
     CDS             4030..5691
                     /locus_tag="OQN12_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCS1655903.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5912..6253
                     /gene="symE"
                     /locus_tag="OQN12_003730"
     CDS             5912..6253
                     /gene="symE"
                     /locus_tag="OQN12_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCS1655904.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6300..>6617)
                     /locus_tag="OQN12_003731"
     CDS             complement(6300..>6617)
                     /locus_tag="OQN12_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655905.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1950 a         1580 c         1526 g         1561 t
ORIGIN      
        1 atagccaggc tattgatttt atgtacaatc agaatcgtcc tgataaaagc gagaaccccc
       61 catggcggaa ctgaacctaa gtaacctgac ggaagcagac atcattacca aatgcgttat
      121 gccagccatt ctcaatgcag gctgggacaa cacaacgcaa atcagacagg aggtcaaact
      181 ccgggacggt aaagtcatcg tacgtggtaa agttgcggca cgcagaacgg taaaatctgc
      241 tgacatcgtg ctgtaccaca agccaggcat tcccttagcc gtgattgaag caaaagccaa
      301 caaacatgaa attggcaaag ggatgcaaca gggcattgaa tatgcgcgcc tgctggacgt
      361 tccttttgtt tttgccacca acggtgatgg ctttatcttc cgcgatgcca ccgcagccga
      421 aggtgaatgc cttgaaaagc aaataacgct ggatgacttc ccctcccccg ctgaactctg
      481 gcaaaaattc tgcctctgga aaggatacac acaagctcag cttccggtga ttactcagga
      541 ctattacgac gatggcagcg gcaaatcgcc tcgttattat caacttcagg caatcaacaa
      601 aaccattgaa gccgtctcca acgggcaaaa ccgcgttctg ctggtcatgg cgaccggaac
      661 agggaaaacc tataccgcat tccagatcat ctggcgcctg tggaaatcaa aaaataaaaa
      721 acgcattttg ttccttgccg atcgcaacat tctggtcgac caaaccaaaa ataatgattt
      781 ccagccattt ggtacggcaa tgaccaaagt cagcggacgc accattgatc ccgcttatga
      841 aattcacctc gcgctctatc aggctataac tggcccggag gaagaccaaa aagcgtttaa
      901 acaagtcgca ccagatttct tcgatctgat cgtgatcgac gaatgccatc gcggcagcgc
      961 atccgaagac agcgcctggc gagagatcct tgattatttc agttccgcca cccaaattgg
     1021 cttaaccgcc acgccaaaag agacgcatga agtctccagc acggattact tcggcgatcc
     1081 ggtttacgtc tactcactaa aagaagggat cgaagacggc ttcctcgctc cttataaagt
     1141 tgtccgtgtt gatattgatg tcgacctgca aggctggcgc ccaaccaaag ggcaaactga
     1201 cttaaacggc gaagtgattg acgatcgtat ctataaccag aaagatttcg atcgcacgat
     1261 ggtgatcgac gaacgcacag aactggttgc cagaaccatt accgactacc tcaagcgcac
     1321 caatccgatg gataaaacca tcgtcttctg taacgacatc gatcatgcag aacgtatgcg
     1381 ccgcgccctg gttaatctca acccgcagca ggtgaaaaag aacgacaaat acgtcatgaa
     1441 aatcaccggc gatgatgaaa ttggcaaagc tcagttggat aacttcatca acccgaaaaa
     1501 accgtacccg gttatcgcga ccacttcaga gctgatgacc actggtgtgg atgctaaaac
     1561 ctgcaaactg gtagtactag accagaacat ccagtcgatg accaaattca agcaaattat
     1621 cggtcgtggt acacgcatcg acgaacgtta cggcaaactc tggtttacca tcctcgactt
     1681 taaaaaagcc accgaactgt ttgccgatga gcgtttcgat ggcattcccg aaaaagtcat
     1741 ggataccaca ccagaggata tcgccgatcc agaatctgat tttgaagaga aactcgaaga
     1801 aatcagcgaa catgacgacg aacaggtaac aggcgttgat gaaccgcctg cgccaccata
     1861 ccaggttacc gataccgatg atgtcggccc atttccggaa gaagacgaga agaaaatccg
     1921 caagtttcac gtcaacggtg tagcggtggg cgttattgca cagcgcgttc agtattacga
     1981 cgccgacggt aaactggtta ccgaatcctt taaagattac acccgcaaaa cactgctcaa
     2041 agaatatgcc tcgctggatg actttacccg caagtggcag gacgccgatc gcaaagaagc
     2101 gatcattcac gagctggagc aacaggggat catctgggaa gtactggcag aagaagtcgg
     2161 taaagatctc gacccgttcg acatgctttg ccacgtagtg tatggtcagc cgccgttaac
     2221 ccgcaaagag cgcgccgaga acgtgcacaa gcggaactac ttcacaaaat actctgaagc
     2281 agcgcaagcc gtgctcgata atctgctgga taaatacgcc gatgcgggcg tacaggagat
     2341 cgaaagtatt caggtgctga aacttaaacc attcgacagc atgggcacct taccggagat
     2401 tattaaaacc ggatttggcg accgtaacgg gtataatcag gcgctcagcg agctggaaaa
     2461 cgaaatctac caattaccgc cccgctctgc ttaacaactc tcttttgcgc cttctgcaac
     2521 catcatgttg cagaaggcct tgtttttcat ggaatacaac atgtctatca gctcagtaat
     2581 caaatcatta caagatatta tgcgcaaaga tgccggtgtg gacggcgatg cgcagcgtct
     2641 cggtcagctc tcctggctgc tgtttttgaa aatcttcgat gcccaggaag aggcgctgga
     2701 actggagcag gataactatc aatatccgat cccacagcgt tatttgtggc gcagttgggc
     2761 cgccaacgcg cagggcatta ccggtgattc cctgctggaa ttcgttaatg atgatctgtt
     2821 tccggcgttg aaaaacctca ctgcgcctat cgataaaaac ccacgcggct acgtggtgaa
     2881 gcaggcgttc agcgatgcct ataactatat gaaaaacggt acgctactgc gccaggtgat
     2941 caacaagctg aacgaaattg actttaccag cgccagcgaa cgccatctgt ttggtgatat
     3001 ttacgaacag atccttaaag atctgcaatc tgcgggcaat gcgggcgaat tctatactcc
     3061 acgcgccgtc actcgcttta tggtggatcg cgttgatccg aaactcggcg aatccattat
     3121 ggacccggcc tgcggtacgg gcggttttct tgcctgcgcg ttcgatcatg taaagaacaa
     3181 atacgtgaag agcgtcgccg atcatcagac actgcaacaa cagatccacg gtgttgagaa
     3241 aaaacagctt ccgcacctgc tggcgaccac caatatgctg ctgcacggca ttgaagtacc
     3301 ggtacaaatt cgtcacgaca acaccctgaa taaaccgctt tcctcctggg atgagcaact
     3361 ggatgtcatt gttaccaacc cgccgtttgg tggcacggaa gaagacggta ttgagaagaa
     3421 ctttccggca gagatgcaaa cccgcgaaac ggcggatctg tttctgcaac tgattgtgga
     3481 agtactggcg aaaaacggtc gtgcggcggt ggtattgccg gatggcacac tatttggcga
     3541 aggtgttaaa accaaaatca aaaagctact taccgaagag tgcaacctgc ataccatcgt
     3601 gcgtttaccg aatggtgtgt ttaatcccta taccggcatt aaaaccaacc tgttgttctt
     3661 taccaaaggt cagccaacca aagagatttg gttctatgaa catccgtatc cggcgggcgt
     3721 gaaaaactac agcaaaacca agccgatgaa gtttgaagag tttcaggcgg agatcgactg
     3781 gtggggtaac gaggccgatg gttttgccag ccgcgtcgag aatgagcaag cgtggaaagt
     3841 cagcattgat gacgtgattg cgcgcaactt caatctggat attaaaaacc cacatcaggc
     3901 ggaaaccgtc agccatgatc cggacgaact gttagcgcag tatgcaaaac agcaggcgga
     3961 gatccagacg ctgcgtaatc aactgcgcga tattcttggc gctgcgctgt ctggcaagga
     4021 ggttaactaa tgagtgtgga aaagctgatc gttgatcata tggaaacctg gacctcggcg
     4081 ttgcaaaccc gttccaccgc cgggcgcggc agctccggta agattgattt gtatggtatt
     4141 aagaaattgc gtgagctgat tctggaactg gcggtgcgcg gtaaactggt gccgcaggat
     4201 ccgaatgatg aaccggcgtc ggacctgctg aagcgtattg cggcggaaaa agcagagctg
     4261 gtgaagcaag gcaaaattaa aaagcaaaaa ccactgccag aaattagtga ggaagagaag
     4321 ccgtttgaat tgccggaggg atgggagtgg gtgcacctcc ctgatattta ttgctcaatt
     4381 tctgagtcat ctagaaaaat aaaatcctcc gaaatactac ctgagggaaa atatcctgtt
     4441 atcgagcaaa gccaagaatt tatttctggt tattgcaaca atgaatgtct gttaataaag
     4501 ttaaataatc cagttatagt ttttggtgat cacacaagga atattaaatt tattgatttt
     4561 gattttgttg tcggcgctga cggtgtgaag attttatcac ctatattaat ttgtgagcgt
     4621 ttcttttttt ggcagttacg atcatttaaa ttagatgtaa gaggatacgc gagacatttt
     4681 aaagtattaa atagctgctt atttgcatta ccccccattg cagaacaaga gcgaatagtt
     4741 gaaaaagtta gctctttgat gtccctctgc gaccaactag aacagcaatc cctgaccagt
     4801 ctggacgcac atcagcaact ggttgaaacc ctgttgggaa cgcttacaga cagccaaaac
     4861 accgcggaac tggctgaaaa ctgggcgcgt attagcgagc atttcgacac cctatttacc
     4921 accgaagcca gcgtggatgc gttaaaacag accattctgc aactggccgt aatgggcaaa
     4981 cttgtgccgc aggatccgaa tgacgaaccc gcctctgaac tgctcaaacg tattgcgcag
     5041 gaaaaagctc aattggtgaa agaagggaaa ataaaaaaac aaaaaccact gccgccaatt
     5101 agcgatgagg aaaaaccgtt tgaacttccg gaagggtggg aatggtgtcg tattggtaat
     5161 atagttaata ttaaatctga attagtatca ccaaaagatt atttaaattt atatcaagta
     5221 gcacctgata ttattgaaaa gggcactggg agagtgatat caaagagaac agtgaaagaa
     5281 tcaggagtca aagggccaaa tagtcgcttt tataaaggac aaattgttta ttctaaaatc
     5341 cgcccgtcat tatccaaagt ttttttagca gagtataatg gtctttgcag tgctgatatg
     5401 tatccattag actgttacat taatccaaat tacttattga aatatatcct ttccattcca
     5461 tttcttatgc aagtcaaaaa agcagagaac agaataaaaa tgccaaaact taattctgac
     5521 agtttttaca atattattgt tgcgattccc ccctataatg aacaacaagc aatatttgat
     5581 aagataaatt ctattgaagc agtatgcaat ggtcttattt cttatattgg gatttatcat
     5641 aaaacccaac tgcatcttgc agacgcactc actgacgcag cgataaacta attccctcgc
     5701 cctcacaaaa ccaataaagc ggctaaatag ccgctttatt cacatcacca aaatttatat
     5761 ttcacgctaa ttttcatctt attttcatta atttttagct ggactttccc tgtatttact
     5821 gatgatgtat ccaggtattt agcacggtgc ggatgtgcgc caacacactc gcaccgctaa
     5881 tcacaatcac tatttataga gaatagtaat catgactgac acgcattcta ttgcacaacc
     5941 gttagaagca gaagtctccc cggcaaataa tcgtcaatta accgtgagtt atgcgagtcg
     6001 ttacccggat tacagccgta ttcccgccat caccctgaaa ggtcagtggc tggaagccgc
     6061 cggttttgcc accggcacgg cggtagatgt caaagtgatg gaaggctgta ttgtcctcac
     6121 cgcccaaccc gccgccgccg aagagagcga actgatgcag tcactacgca aggtgtgtaa
     6181 gctgtcggcg cgtaaacaaa ggcaggtaca ggaatttatt ggggtaatta caggtaaaca
     6241 gaaagtcgcc tgatacattg acccggtagc gccagtgcta ccgggtatta ttcgccgcgt
     6301 tataccaacg cgctctccat ctgtttttca aatgcctgac gaattaatcc cataatgtaa
     6361 ggaagttgtg cgaggtcgct gaaaccaatt tctacatcgc cattacccca gcgcccaaca
     6421 tttgtcacat ctttggcaat acctttcgga tcgaccagtt catgaaactg catattaagc
     6481 gacaatcgca gtcggctttt ttgcggcaca acatcaacaa aattcgtttc cgctttaaac
     6541 gcaatatata gcttcaatac ttcctgcgtg atccctgcgt ccaggcgcat aacttcatcg
     6601 cgaagatgat cgaataa
//
LOCUS       DAJVRP010000152         6538 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVRP010000152 DAJVRP010000000
VERSION     DAJVRP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.152"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(152..2155)
                     /locus_tag="OQN12_003732"
     CDS             complement(152..2155)
                     /locus_tag="OQN12_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655906.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2152..3288)
                     /locus_tag="OQN12_003733"
     CDS             complement(2152..3288)
                     /locus_tag="OQN12_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1655907.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3281..5560)
                     /locus_tag="OQN12_003734"
     CDS             complement(3281..5560)
                     /locus_tag="OQN12_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655908.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5571..>6538)
                     /locus_tag="OQN12_003735"
     CDS             complement(5571..>6538)
                     /locus_tag="OQN12_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCS1655909.1"
                     /translation="AARAFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEP
                     GTAKSLLSELLATSISGDAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPL
                     YQGMRDGKIVRFEEITRTPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIAT
                     ANTRDRGVNEMSAALKRRFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAV
                     LELLVRTFRDLRANGEKKTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLV
                     DCIAGTIVKDNEEDRARLRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1498 a         1803 c         1755 g         1482 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat taccaactgg
       61 tgactctcac caggggctat ataggatatg cctaataccg tggcgtgagc agtccggaac
      121 tggagtagaa ctcttagtaa aaagcactat ttcatccttg ttgctgaagc atggggaata
      181 attgttcgca aagcaaaaca ccgttattca ttgcttctac ccgtgcctcg ctttctgtat
      241 tacgaaattg tgccaacaca tgtgccagcc gataaaaacc caccgcggtg aggtcattcg
      301 ccagcaactc tgcctgatta atagcgctct gttcctgata gcgccagccg ttatggagca
      361 gttgaataag taacgcctgg cagcgcatca gcaactgatg agcagtagac ggcacaggca
      421 gaacgctggc agaagatagt gataccacca caggcgcagt ttctgcgtcc agcgcccagg
      481 cacgagtctt tgtcatcatt acctgtggtt ccagtgtcaa ttgcccatca acaaaactga
      541 caaagccaga aaccagacac acggggtcgt ctgtttgttg caaaagcgcc gccatgcgtt
      601 caatggcata aggcgcgctg gctgaggctg gcagtgataa cgtcagcaga ttatcttcac
      661 cttcgccgct gattacctgc gcatccagcg tctggcggct gctatcccaa ccgagcgaaa
      721 tacactcagc gaccggcaga ataaataagt tatcgacctg attaagaggc cgtatgcagg
      781 cggggggacg ctggcgtaaa tattcccgca aagccacaat gcccggctgg cgtaacggcg
      841 cgctcaacat ttgccaggca tcaggcgaca gcggtacaac gctgcttaag cggttgcggg
      901 tagctaacag cagctcgcca tcggcactgc gttttgctgc ttgtgaaaca atttgcccac
      961 ccgccagtgc gccagcctga aaactaaaca gccgacgcgt agctgccggt gagtcttcct
     1021 gttcacttcg cggccaactg cgcgaaaggt gcaaaatact gccggtgtcg ggatcggtaa
     1081 accagatgcg taaaccataa tgctcaatat cctgccagca acgcatacct aaagacacca
     1141 gccgcagatg atcaagcttt gcttctccgg caatgccaga gccaacgacc gtgcgccacg
     1201 gcacaggagg aacttcacca acactgtcgc tccgggccat ctcttgtgcg caatttaatc
     1261 gactgtttaa tgccgcaagc tgacgtaagc attctccggc atgatagtgg ctggcgcggg
     1321 cgtggaaggc atcaacgctg gcgcgcagtt gccgtagcga ttcactcacc catcgccagc
     1381 tgcagcgttc cgctgcttgc tgcgcgcggc tgaaagcggc ctcgtagtga ataagcggct
     1441 ggctgatgcc gctcagccat aatgcctggc ttagttgttg aacatattga cgacacgttt
     1501 tgccttcttc gctggcaaac ggatcgtcag atgatgtgac gtgttcgctg cgcatctgcc
     1561 agattaaatg agtcaattcc gcttgctgcg cttttgcctg gacaaaggcc tgtactgcca
     1621 gtacgacatg ttcgcaaagt gtgccttcaa tacaatcaca acgggcgaaa cgaatactgc
     1681 tgcgggaata aaaacgcaca tcgctcatcg gtaagcgggc agagggaatt tcacccggcg
     1741 cacagaacaa ctcaatggtg atgcttttag cgaccagcgc ctgtgcgcgt ttgcgggtgg
     1801 catcgggaag ggtagccagt tcttccagcc agattgccgg atcccattcc tcttcttttc
     1861 ccgtaggctg ggcggtagca caaagtcgtt gataacttaa caccagcatc acgcgatgac
     1921 ggcacatgcc gctggcccca taggtgcact gagcctcttt cagtgcctgg ccgttcgcca
     1981 gctgggtacg gacaccgtca ctgaaggtgg cgattaaagc gctgttctca tggctgattt
     2041 ccgggacgtt gccattttcc agttccttaa ggctgcgctt aacaaaaccg gcattgctta
     2101 acgccgtcag tgcctgcggt gtcagttcta ataattccgg acgtagtgaa ttcatgactg
     2161 aagattctcc gcaagccatg atgccagctc gcccggtgtc atggcggcta tttgtgcgcc
     2221 gacattaatc agcgcctggg ccgtatcgcg gtcatagcaa ggtgttgctg tgctatcgag
     2281 cgctgccagt cccagcactt tgatgccgct ctggacacac tttttcactt ggtgcgtcag
     2341 caatgatgat gaaccccctt cataaaaatc gctcacgagg ataatgacgc tttttgcagg
     2401 ttgttcaata agttgccgac catactccat ggcactggcg atattggttc cgccgcctaa
     2461 ttgcactttc attaataact ctaccggatc ggcaacgtct gccgtgagat caacgacgct
     2521 tgtgtcaaac gccaccagat gggtacgaat gccgggtaac tgccataaac aggccgccat
     2581 caccgcagag tggatcaccg aatcgaccat cgatccgctt tgatcaacca gtaagaccag
     2641 ttgccattgc tcgctttggc gtttaatgcg gctgttaaag cgggggaatt cgatatacaa
     2701 cttgccgtgt tgcgggtgcc agtgttgcag gttggcgcgc agagtacttt tgaaatcaaa
     2761 gtttcgcgcc agtggaataa atgagcggcg acgacgatcg cggacaccag aaaaagcctg
     2821 acgaacttcc tttgccagtc gcgccataat ttcttccaca acctggcgca caatcctgcg
     2881 ggcggcagcc agtacttcgg ggttcatcag atgtttggtg tgcaaaacgg cgcgtagcag
     2941 gctttcggaa ggctgcatac gttccagcac gtcgagattt gtcaccacat cttcaatgcc
     3001 gtagcgcagt acggcatcgc tttccagccg ctcaatcacc tgttgcggaa acagcgtgtg
     3061 aatactgttg atccactcag gagtggtgag atttgagcca cctaatccac tagagcgttc
     3121 accacgctgg agccgttcag gatcgcgccc atacagccac tccagcgcgt gatctatctg
     3181 ccgggcgttg tcatccagcc cacaaagcgt cgtttctgcc gcttcgccaa gaattaatcg
     3241 ccagcgttgt agctcacggg tggtcagaag atcgttcagt tcagacatgg aaaactcccc
     3301 agtgttgcag cgatgccagt gcctgctgtt cgagttgttg atgatgtgca atggcttgcg
     3361 gtggacaatg caacggcatt tgcagtgccg aaacgggaag ttgcgccagt tgataatgct
     3421 caagcacctg atgcgccagc gtcccgcgtt ctcgtggcgg tagccaggcc attgccgcgc
     3481 gtaaatcggg cagggcattg gtaaaatcgg catcacttag ttgatttaaa tgactgctga
     3541 aaccggcgat aaatgccggc tgacaggcca gttgatgacg ggctaatgcc agcaaaccgt
     3601 gcagcgcctc gccggattgt gccggggata actgcgccag catcgtcagc gccgcttcgg
     3661 cactagcgtt gggatgttcc agacgcatca gcgcaccaag agtcgcgccg cgatccagtg
     3721 ccaggacatg aatcgcctga ctgtgtcgct ccagcaaggc taccgccgca gaaagcgaaa
     3781 cgccggggag ttgcacaccg ctatgtagat cgcgcaggat atgacacaac gcttgccagc
     3841 tatgcaagtg agcgtgaaac tccttttcat ccggtctgcc gttggattca cacagccaca
     3901 gcgtgcgatc gatagccgcg cataacgtcg tttgtaatat ctgcgcgcct tgcataccgc
     3961 taatttcatc cagccgccat aaggcatata gcacttccag cgcctggccc atttcggcaa
     4021 attgattttc ctgggcgatt aataatgtga gttgctccag taattgttga ctgaaggacg
     4081 ccagacccgc tgacgccgct tggctaagac atgtggtaat gctgccgatt ccacccgcgt
     4141 ccagcatatc ggcttctaat ttatggcgtg cggcttcctg gagtgtggca ccaaagcagg
     4201 cggcctcaat caatgccgca tgctggctaa gcgggcgggt taatttccag cgttcttcac
     4261 cgttgcctgc aagtgtcagt gtgcttccct gctggcgtac aatccctggg atctccagta
     4321 ttgccagccg atgtaacacc tgactttgcg ctagtccatt ggggttaaag cgatttaagg
     4381 taagctccgc cggaagagag attgcggtac gttccagttc gcaagtgaca tcttttggca
     4441 gaggcggttg tggcgtagaa ggggcaagtt taccgaatcc gtcacccgct aatgtgtcta
     4501 tcaacgttag cagaatcgga tcggtatcgg gatgaataac gccgcgatag ctccacggca
     4561 acggcgcatt cagggcttct ttaatcagcg atcctgctat ggcatccagc cagtcagtgc
     4621 gtaacggtag tgtatgaccg cgcaactgtg ccagtgccat cgcatgcaga tgagcggcag
     4681 ccatatccgc ggtcgaagca ggcaacttgt gctggcgcaa acgggtgaga accgttttta
     4741 gcagttgttc tcccgcctgc tgtaagcccc actgccagca ccagttttgc cagaccgggg
     4801 caggcattcc tgaaaggtat cctgccagca catcaaggcg cttttcactg tagggggtga
     4861 gataacaacc tgtaacggca tctgccagcg acggcaattc tggcgtgtta attacctgcg
     4921 gacattcgcg ccacattttt gccagtgccg gagcgtgcca gccaccgcag acaactaaca
     4981 cgtcgccatt attttgctgc atcgcccatc caatccagcg ggccataaag gcttcgcgct
     5041 gacgatttag agcatcgccg ggggagtcgc cccgcagttg ggcaaaatag tgcgccagtg
     5101 cagagggtaa tgcagtttgc tggctttcat cttcgaacaa gtgatcccac agggtgtcgc
     5161 tgttatccat gcgggtggca cgcagcagca aggtctggct ttcctcttgc gtatcaggcg
     5221 aatcgtcctc ttcttcactc tgcgcccagc aaggcaaatc gatgaagtaa gtttgtgcct
     5281 gaatacgacg atcggcttgt agcgcctgcc actccggcga aaattcagca aatggcgtcc
     5341 aggcaccacg cccgggggct gcaccgtcct gatactggca ataactgtaa atagctaccg
     5401 gaagctggtg ggctaaaaac agttcgtcta cccggtcatt aaaatcagca gggccttcaa
     5461 tcaacacgta tcgtggccgc tggctttcga ttaacgattt caccagccgg gcgcaggccg
     5521 gactatgatg ccggatgccg acaattaacg gctcgctcat gcatctttcc tcacggcagg
     5581 cggtggcggg cttgataata agcctgccag tgagcttctt tatgtgtggc gacacgctgt
     5641 tcgaagtaac ggcgcagacg agcgcgatct tcttcgttat ctttgacgat ggttccggca
     5701 atgcagtcca ccaggtcggc tggctcacct gcgcgattcg ctaaaaacca ggcgcggacg
     5761 cctacggcgt gggccacgtt cacggcttca gcggtggaca taatcgctgt tagtgtatcc
     5821 attgacgttt tcttttcgcc gttggcacgc agatcgcgga aggtgcggac cagtaactcc
     5881 agtaccgcgt caggcacttt atgcggaata ccgctatggg ccagcaaacg cgccgaagca
     5941 ctggcgacaa gctccagctc ctgggcgaaa tccataatcg gaaagacagt ttcaaaatcg
     6001 aagcgacgct ttagcgcagc actcatttca ttgacgccgc gatcgcgggt attggcagtg
     6061 gcaataatat taaagccttc tcgcgcatag agctgactgg cttcaccagt gagttccggc
     6121 accgtcatca ccctgtcaga aagcatacct aacagacagt cttgcacttc cagcggtgta
     6181 cgcgttatct cttcaaaacg gacgattttg ccgtcgcgca ttccctggta aagcggggca
     6241 gggaccagcg cttccgttga tggaccatga ttgataagca gggcgtagtt ccagccgtac
     6301 ttgatttgat cttcggtagt agatgcgccg ccctggatgg ttaacccggc atcgccgcta
     6361 atagaggttg ccagtaattc agacaggaga gattttgcgg tgccaggttc ccccaccaac
     6421 atcaaaccgc gtccggtggc aagagtgact aacatacgtt cgacagcgga gggactaatc
     6481 accactttac ggctaatatt ttgcgcgcta tcgccaagga taaaagcacg ggccgcag
//
LOCUS       DAJVRP010000153         6506 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVRP010000153 DAJVRP010000000
VERSION     DAJVRP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.153"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(296..1198)
                     /locus_tag="OQN12_003736"
     CDS             complement(296..1198)
                     /locus_tag="OQN12_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCS1655910.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OQN12_003737"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OQN12_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCS1655911.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OQN12_003738"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OQN12_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCS1655912.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OQN12_003739"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OQN12_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCS1655913.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OQN12_003740"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OQN12_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCS1655914.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..6506
                     /locus_tag="OQN12_003741"
                     /pseudo
     CDS             6040..6506
                     /locus_tag="OQN12_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
BASE COUNT         1464 a         1662 c         1706 g         1674 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgacttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc cactctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgctctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcg
//
LOCUS       DAJVRP010000154         6404 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVRP010000154 DAJVRP010000000
VERSION     DAJVRP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.154"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OQN12_003742"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OQN12_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCS1655915.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OQN12_003743"
     CDS             complement(1433..1759)
                     /locus_tag="OQN12_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCS1655916.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OQN12_003744"
     CDS             1894..2235
                     /locus_tag="OQN12_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCS1655917.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OQN12_003745"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OQN12_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OQN12_003746"
     CDS             complement(2833..3543)
                     /locus_tag="OQN12_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCS1655918.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OQN12_003747"
     CDS             3651..3956
                     /locus_tag="OQN12_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCS1655919.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4478
                     /locus_tag="OQN12_003748"
                     /pseudo
     CDS             4155..4478
                     /locus_tag="OQN12_003748"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5034..5613
                     /locus_tag="OQN12_003749"
                     /pseudo
     CDS             5034..5613
                     /locus_tag="OQN12_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1706 a         1629 c         1501 g         1568 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tccagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaacttcaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc agcccccccg gtggcctcgg
     5701 tctggacttg aggtgggcct atggggggaa gtctgctggc ttaatgtatt agatggtcat
     5761 tcacattttt ctgtcagaaa ccatcggtcc cactgtgagt ccaaacctaa ggacctagaa
     5821 gtccacccac agccttagac agagtgtcga acacatccac accgagtcga ccagagagga
     5881 ccatgtagag acccacagag aggaacacga gtttatagac cagagtcagg agggacttca
     5941 ggtctgcgga agtcatagac ataaagcccc tatctgttat gttatattat tatattaaca
     6001 acaggtccag accattggtc cagaccatag ttagttaatc acagtgatag cccaccacct
     6061 gaagtcctgc tgatattcag tccagactta cctctgctca tggtcttacc tccctcggtc
     6121 tcctgacctt gggtatcgac tacagcttgc tgagcgtcat ctggaggagc tacaggaggc
     6181 gggaccgaag ccccaccttg agtcccacca ccacctgtca ggcccttggc gatggacctg
     6241 aagcctttac taatgctctt acccattggt cttacgcttc ggtcttggtg aacttcaggc
     6301 gtttgactac aggcaggacg gcagtagctg agccagtgcc catcttgacg gtcgtagcag
     6361 cttcagcgac aacgaacact ttaccgagga tttccaagtc gtgg
//
LOCUS       DAJVRP010000155         6374 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVRP010000155 DAJVRP010000000
VERSION     DAJVRP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.155"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            58..1384
                     /gene="pgaC"
                     /locus_tag="OQN12_003750"
                     /pseudo
     CDS             58..1384
                     /gene="pgaC"
                     /locus_tag="OQN12_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1386..1799
                     /gene="pgaD"
                     /locus_tag="OQN12_003751"
                     /pseudo
     CDS             1386..1799
                     /gene="pgaD"
                     /locus_tag="OQN12_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="OQN12_003752"
     CDS             complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="OQN12_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS1655920.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="OQN12_003753"
     CDS             complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="OQN12_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS1655921.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="OQN12_003754"
     CDS             complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="OQN12_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS1655922.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5701..>6374)
                     /locus_tag="OQN12_003755"
     CDS             complement(5701..>6374)
                     /locus_tag="OQN12_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCS1655923.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1534 a         1680 c         1533 g         1627 t
ORIGIN      
        1 accaacctga aatagacctt attcgtcctg agttttcaac agcctggtat ccgaaaaatg
       61 attaatcgca tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc cctatgcgta
      121 gcgtactttc actctggtga actgatgatg aggttcgttt tcttctggcc gttttttatg
      181 tccattatgt ggattgttgg cggcgtctat ttctgggtct atcgtgaacg ccactggccg
      241 tggggagaaa acgcaccagc tccccagttg aaagataatc cgtctatctc cattatcatt
      301 ccctgtttta atgaggagaa aaacgttgag gaaaccatac acgccgcttt agcacagcgt
      361 tatgagaaca ttgaagttat tgccgtaaat gacggttcaa cagataaaac ccgtgccatc
      421 ctggatcgca tggctgcaca aattccccat ttgcgggtca ttcatctggc gcaaaaacag
      481 gggaaagcca ttgcgcttaa aaccggagct gccgcggcga aaagtgaata tctggtgtgc
      541 attgatggcg atgcgttatt agaccgcgat gcggcggcat atattgtgga accgatgttg
      601 tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg ttctaccctg
      661 gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg gtttgatcaa gcgaacccag
      721 cgtatctatg gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc
      781 ctggcagaag tgggttactg gaatgacgat atgatcaccg aagatattga tattagctgg
      841 aagctgcagt tgaatcagtg gacgattttt ttacgagcca cgggcgctgt gctggatatt
      901 aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg cgctgggctc agggcggtgc
      961 agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc
     1021 gctgtttttt gaatactgcc tgacgacaat atgggccttc acctgcctgg tcggtttcat
     1081 tatttacgca gtccaacttg ccggtgtacc gttaaatatt gaattgacac atatcgctgc
     1141 gacacatact gccggaatat tattgtgtac gttatgttta ctgcaattta ttgtcagcct
     1201 gatgatcgag aatcgctatg agcataatct gacttcatcg cttttctgga ttatttggtt
     1261 cccggttatt ttctggatgc tgagcctggc aacgacattg gtatcattta cacgagtcat
     1321 gttgatgcct aaaaagcaac gcgcccgttg ggtaagtcct gatcgcggga ttctgagagg
     1381 ttaatatgaa caatttaatt attacgaccc gacaatcacc agtacgttta ctggttgatt
     1441 atgttgccac aaccatcttg tagacattat ttgctttgtt catattctta ttcgccatgg
     1501 atctgctgac gggttattac tggcaaagcg aggccagaag ccgacttcag ttctattttt
     1561 tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt
     1621 ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc gcaagaatat gcagagagct
     1681 tagcaatacc tgatgagctc tatcagcaac tacaaaaaag ccacaggatg agcgtacact
     1741 tcaccagcca ggggcaaata aaaatggttg tttcagaaaa agcgctagtc cgggcataaa
     1801 cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc
     1861 atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc tcttttccga accggacgat
     1921 cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
     1981 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
     2041 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
     2101 aactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcggttt ccaccttccc
     2161 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcaagtc
     2221 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tcaccaggta agaagccaag
     2281 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
     2341 cagggcctct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
     2401 ggcgaatatc agctgcttac tctcaatagc ctgcagatag tgcaattgcg cttcatttcg
     2461 cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata ccaatggctt ctacgccgct
     2521 catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
     2581 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
     2641 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
     2701 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
     2761 acggctacgc acaccgtttg cggcgtcggt aacacgagta aagaaaggaa gtgagaaaaa
     2821 tacagagtga cgaagttgct accggaggag aaatctccgc gagtggtgga gcgttgctta
     2881 acaaaacatt gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca
     2941 ctgccgggaa cttccgctgc tacttaataa gtacaacaga actcgcattc attgcaacaa
     3001 tatattcact tatatttaac gataaacacc catttcggtg acattaattt ctactggaaa
     3061 gatgacagag tgatgacagt gatggaaaaa gctgtgtgct ttccgcagga ttcgcagggc
     3121 tggtaaaatg tgcctcgtgt ttgccggatt cggcgcgaac gccttacccg acctacaagt
     3181 gattaataat ttgtgcacaa aactcatgcc cgataaacct gcgcatcggg catatttgcg
     3241 tcgttcaaac ctgcaatagc gcccttccca gataatcgtt cgcgtccttc actcccggca
     3301 gcgaaaaaaa ataaccgccg ccgataggtt taacgtattc ctccagcgct tcgccattga
     3361 gccttttttg tactgtcagg aagccttttt ccagatcgtg ttggtagcag acaaacagca
     3421 accccatatc cagttgtccg gagttggtga cccccaacga ataactgtag ccgcgacgca
     3481 gcatcaggct ggactcactc tccgccgtgc ggggattcgc cagccggata tggctgtcca
     3541 gcgcgatcac cttcccttcc gggtcgctgg cgtaatcagg cacatcatgc tcatgctgca
     3601 ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat cgtctgctgt tctttcagcg
     3661 gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg tactgcctga tagctgccac
     3721 cgattgtcca cgcaggctcc tgctgatctg ccgttaccca caccactttt tgcatcaact
     3781 tatcattctg gctatcggga ttggcagtgc cgtctttgaa acccagcaaa ttaatcggcg
     3841 tctctttgcc tttactacgc gccgcgtgat cggaaataaa cccttcccgc ttccagcgca
     3901 cactgagcaa atccggcgtg tgtttgatga tatcgcgcag cgcatggttc accgtgtcct
     3961 gggtgttggc gcaaatctgt agcaacacat caccatgaca taacgccgca tccagcgagt
     4021 cgttggggaa acgcgtcatc ttctgcagct tttttggcat ctgtggcgca aggccaaagc
     4081 gctcatcaaa caatgagtga cccaccgata acgtgatggt gagattatcg ggcgcaatgt
     4141 agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg atttggcgtt tctggtgctg
     4201 ctccgccctg actcagaaaa gcaaaacgct gagtcaacaa acgaaacaac cgctcaagat
     4261 cggctttatc gctggcaagc acatcaaacg ccaccagcat cattgcggcc tgttgtggcg
     4321 tcaggatccc tgcctgatgc tcaccataaa acggctgttt ctcattgcgt gcatccggtg
     4381 aaagcgtgcc cggtgcactt tgcgtttttt gtgcatgagc gaccggacaa cttcccgcca
     4441 gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg ttttcatctt
     4501 tatactgcat aacgcttaat ccagtcccag cacaccgcga agttgcgcca gatcttccgc
     4561 cagcgcagta atcggccctt tcagcgcatt ccggtcggca tcggtcaatt tgtcgtaggt
     4621 ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgacctttt taaagttggc
     4681 atcgactttt gccaacagtt ccgggttggc tttttgcagt tgtggacgca gcagatcgac
     4741 aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt ggctgtagcg
     4801 atcttcttca ccgctgattt tgctggctgc cacttcctca atcagtccgg ctgcgccgcc
     4861 gaccactttt gaaggtggga aagccagttc actgatgcgt ttttgcaaat cgaccacatc
     4921 ggtataaagc tgctcagcgt actgatccat ccctttggtg gtgttgtcgc caaacaatgc
     4981 tttttccaga cggtggaaac cagtgaattt tgggtcggcg gctttttgct cgtaatcatc
     5041 ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg gttcaatacg
     5101 ctcatagtgc tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat cgcctgcttt
     5161 aatcgcgtcg gtaaaggctt tggtgtcggt caccagctgc gtggtttccg ccatgacata
     5221 cgctttatat gcagtaattg caccaccaag acttaacagc gcatcacttt gcgccgcatc
     5281 cgccgttgcc tcacctttga cgatcaactt ccctttcggg ttagtcagca gaccgcaggt
     5341 catatcgtat tcgccaggct gtaaattcgc cgtcattttc tggctaaaac caggggcgat
     5401 attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca gcgccttctg
     5461 gctatggttc tgaataatga actgtgtttt cccggcgtta accgtaatgg tcatcggttc
     5521 gcactgctta tccgtcacgg tcactttgac ctgcggcaca tcagcggcgt tagccataaa
     5581 agcagaagaa aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga agttaatggt
     5641 catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc gtcgtatttg
     5701 ttacgcggag cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg ccaccagcgc
     5761 cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggtgctt cctgatagcc
     5821 aaaaatccct tccatcagcg tgccaaacag cgagtgagtt gagaggaccg cgctcatatc
     5881 gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc gaatggcacc
     5941 agccgccagc cctgcggcga cgaagagaat aaacaggctg gtccatttaa aaaatgcacc
     6001 aagattgagg cgaataccgc cccagtagag caggaagccg agcaccacgg cagtagcaag
     6061 accgagcatt gcacccagcg gcggccagat cccgacatct tgttgaaatg ccgccagcag
     6121 gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca tcaccagcgc
     6181 ccagccatga tgatttccac gctgcaatgc gctatcgact gcctgttcca gttgcacttt
     6241 gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca ccacggcgat
     6301 caccgccacg ataccttcaa acagttcctg ctctttttgc ggaaattcgc cggtggtttc
     6361 gttaatgaag atcc
//
LOCUS       DAJVRP010000156         6335 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVRP010000156 DAJVRP010000000
VERSION     DAJVRP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.156"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            254..1642
                     /gene="narU"
                     /locus_tag="OQN12_003756"
     CDS             254..1642
                     /gene="narU"
                     /locus_tag="OQN12_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCS1655924.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINDGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1724..5464
                     /gene="narZ"
                     /locus_tag="OQN12_003757"
                     /pseudo
     CDS             1724..5464
                     /gene="narZ"
                     /locus_tag="OQN12_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5461..>6335
                     /gene="narH"
                     /locus_tag="OQN12_003758"
     CDS             5461..>6335
                     /gene="narH"
                     /locus_tag="OQN12_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1655925.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1493 a         1685 c         1662 g         1495 t
ORIGIN      
        1 attcacgatc tgaagggttg aaacatgaca tacgttcaaa gattaacccc cgtttgaagg
       61 ttagcttgtt agttacattt agtaacacat atctggctac ttaatcccca ttctgccccc
      121 gcggttacgc tttcttatct attctttcaa tcagcacttc acccaattca tccttaattc
      181 taataactgg ctattgcgcc ttacctcctg ttttgtcttt tttcattacc aatgtgtgca
      241 tatgaggaac aatatggcac tgcaaaatga gaaaaatagt cgttatcttt tacgcgactg
      301 gaaaccagaa aatccggcct tctgggaaaa taaaggaaaa catattgctc gaagaaacct
      361 ctggatatca gtcagttgtc tacttcttgc cttctgtgtc tggatgctat ttagcgcagt
      421 taccgttaat ctcaataaaa tcggttttaa ttttactacc gatcaactct ttttattaac
      481 cgcattaccc tccgtttctg gcgcattatt gcgtgttccc tactccttta tggtgcctat
      541 attcggtgga cgccgatggc cggtttttag tactgcaatc ctgattattc cttgcgtctg
      601 gctcggaatt gccgtgcaaa atccgaatac tccttttggg atatttatcg ttatcgcttt
      661 gctatgcggt tttgcaggtg caaactttgc ttcgagcatg ggcaatatca gtttcttctt
      721 tccaaaagcc aaacaaggga gcgctcttgg gattaatgac ggattaggaa acttaggtgt
      781 aagtgtaatg cagctggttg caccgctggt catttttgta cctgtatttg cctttctcgg
      841 cgtcaatggc gtaccgcagg ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat
      901 ttgggtaccg ctactggcga ttgccacgat cgccgcctgg tcagggatga atgatatcgc
      961 cagttcacgc gcctcaattg ccgaccagct ccctgtctta caacgcctgc atctctggct
     1021 gctgagcctg ctttaccttg ccaccttcgg ttcgtttatc ggtttttctg cgggttttgc
     1081 catgctggca aaaacccagt tcccggatgt gaatattctg cgcctggcgt tctttggccc
     1141 atttattggt gccatcgcgc ggtcggttgg tggtgctatt tccgataaat tcggcggcgt
     1201 gcgggtgacg ttgatcaact ttatttttat ggcgattttc agtgccctgc tgttccttac
     1261 cttaccgggc acaggctccg gtaatttcat cgccttttac gccgtattta tggggctgtt
     1321 tctgaccgcg ggtctgggaa gtggttctac tttccagatg atcgccgtca tctttcgcca
     1381 gataaccatt tatcgggtga agatgaaagg cggtagtgat gagcaagctc agagagaagc
     1441 cgtcaccgaa acggcggcaa cgctgggctt tatctcagcc attggcgcag tgggcggctt
     1501 ttttattccg caggcatttg gcatgtcgct caatatgacc ggctctccgg tcggcgcgat
     1561 gaaagtgttt ttaatcttct acatcgtttg tgtgctgctg acctggctgg tttatggtcg
     1621 gcggaagttc agccaaaaat aagagatcga tgcctgatgt acaaccatgt cgcatccgac
     1681 aaggcatcag atcccaattc attcctggag caggagtcat gtcatgagta aacttttgga
     1741 tcgctttcgc tacttcaaac aaaagggcga aacctttgcc gatggtcacg gacaggtgat
     1801 gcatagcaac cgcgactggg aggacagcta tcgccagcgt tggcagttcg acaaaatcgt
     1861 gcgttccacc cacggcgtta actgtacagg ctcctgtagc tggaaaatct acgttaaaaa
     1921 tggtctggtg acctgggaaa tccaacagac cgactacccg cgcactcgcc ctgacctgcc
     1981 caatcatgaa cctcgcggct gcccgcgtgg cgcaagttac tcctggtatc tttacagcgc
     2041 taaccgcctg aaatacccgc tcattcgtaa acgactgatt gaactgtggc gcgaagccct
     2101 caaacaacac agcgatccgg tactggcgtg ggcatcgatt atgaacgatc cgcaaaagtg
     2161 cctgagctac aaacaagtgc gtgggcgcgg cgggtttatc cgctccaact ggcaggaact
     2221 aaaccagctg attgccgccg ctaacgtctg gaccatcaaa acctacggcc cggatcgcgt
     2281 tgccggtttc tcgccgatcc cggcgatgtc gatggtttct tacgccgccg gaacgcgtta
     2341 tctgtcgctg cttggcggca cctgttaaag tttctacgac tggtattgcg acctgccgcc
     2401 cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg gaatctgccg actggtataa
     2461 ctccagctac atcatcgctt gggggtctaa cgtaccgcag acacgtacgc cggacgccca
     2521 cttctttacc gaagtacgct acaaaggcac taaaaccatc gccattaccc ctgactactc
     2581 tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa caaggcactg atagcgccct
     2641 ggcgatggca atggggcatg tgattttaaa agagtttcat ctcgataatc ccagcgacta
     2701 ctttatcaac tactgccgcc gctacagcga catgccgatg ctggtaatgc tggagcctcg
     2761 cgacgatggt agctacgttc ccgggcgcat gatccgcgca tctgacctgg tggatggact
     2821 gggcgaaagc aacaatccgc agtggaaaac cgtagcagtt aataccgcag gtgaattggt
     2881 agtgccgaac ggttcgattg gtttccgctg gggagaaaaa ggcaaatgga atctggaatc
     2941 cattgccgcc ggtacggaaa ccgaattgtc gttaaccctg ctcggtcaac atgacgctgt
     3001 tgcaggcgtg gccttcccct actttggcgg cattgaaaat ccgcattttc gcagcgtaaa
     3061 acacaatccg gtgctggtgc gccaattgcc cgttaaaaac ctgacgttag tcgatggcaa
     3121 cacctgtccg gtggtcagcg tttatgattt ggtactggcg aattacggcc tcgatcgcgg
     3181 gctggaagat gaaaacagcg cgaaagatta cgctgaaatc aaaccgtaca ccccagcctg
     3241 gggtgagcaa attaccggcg taccgcgcca gtatattgaa accatcgccc gcgaatttgc
     3301 cgataccgcc cataaaacgc atgggcgctc aatgattatc ctcggcgcag gtgttaacca
     3361 ctggtatcac atggacatga actaccgcgg gatgatcaat atgctgatct tctgcggttg
     3421 tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggc caggaaaaac tgcgcccaca
     3481 aaccggctgg ttgccactgg cctttgcgct cgactggaac cgaccaccgc gccagatgaa
     3541 cagcacctcg tttttctaca atcattccag ccaatggcgc tatgaaaaag tctctgctca
     3601 ggagttactt tcaccgctcg ccgatgccag taagtacagc ggtcatctga ttgatttcaa
     3661 cgttcgcgcc gaacgtatgg gctggctacc ttctgcgccg cagctggggc gtaacccgct
     3721 cgggattaaa gctgaagccg acaaggccgg attatccccc acagaattta ccgcccaggc
     3781 gctgaaatcg ggcgatttac gtatggcctg cgaacaacca gatagcggca gcaatcatcc
     3841 gcgtaatttg tttgtctggc gctctaacct gcttggctcc tccggcaaag gccacgagta
     3901 tatgcagaag tatctgctgg ggaccgaaag cgggattcag ggcgaggaac tcggtgccag
     3961 cgacgggatc aaaccggaag aagtcgagtg gcaaactgcg gcgattgaag gcaagctcga
     4021 cctgctggtg acgctcgact tccgcatgtc cagtacctgc ctgttctccg atatcgttct
     4081 gcccaccgcc acctggtatg aaaaagacga tatgaacacc tcggatatgc atccatttat
     4141 tcatccgctt tctgcggcgg tcgatccggc ctgggaatca cgcagcgact gggaaatcta
     4201 caaaggtatt gccaaagcat tttcgcaagt gtgcgtgggc catcttggca aagaaaccga
     4261 cgtggtatta caaccactgc tgcatgactc tccggcagag ctctcacagc cgtgtgaagt
     4321 gctcgactgg cgcaaaggcg aatgcgatct gatcccgggt aaaaccgcgc cgaatattgt
     4381 ggcggtggag cgcgactacc ctgctacgta tgaacgcttt acctcgctcg ggccattgat
     4441 ggacaaactt ggcaatggcg gtaaagggat ttcgtggaat acccaggatg aaatcgattt
     4501 cctcggcaaa ctcaattaca ccaagcgtga tggcccggca caggggcgtc cgctgattga
     4561 caccgccatt gacgcttcag aagtgattct ggcactggca ccggaaacca acggtcatgt
     4621 tgcagtcaaa gcgtggcagg cgctgggcga gatcaccggg cgcgaacata cccatctggc
     4681 gctgcacaaa gaggacgaga agattcgctt tcgcgatatt caggcgcagc cgcgtaaaat
     4741 tatctccagc cccacatggt ccggtctgga aagcgatcac gtatcctata acgcgggata
     4801 caccaacgtt catgagttaa ttccgtggcg cacgctgtcg ggacgccagc agctctatca
     4861 ggatcatccg tggatgcgtg cttttggtga aagcctggtg gcttatcgcc cgcctatcga
     4921 cacccgtagc gtcagtgaga tgcgccagat accgccaaac ggcttccagg aaaaagcact
     4981 taacttcctg acgccgcacc agaaatgggg cattcactca acctacagtg aaaacctgct
     5041 aatgctgacg ctctctcgcg gtggaccgat tgtctggatc agcgaaacag atgcccgaga
     5101 actgaccatt gtcgataacg actgggtgga agtatttaac gccaacggcg cgctgactgc
     5161 ccgcgcggtg gtcagccaac gtgtaccgcc gggcatgacc atgatgtatc acgcccagga
     5221 acgcattatg aatattcctg gttcggaagt aactggcatg cgcggcggca ttcataactc
     5281 ggtcacccgc gtttgcccga aaccgacgca tatgattggc ggttacgcgc agctggcctg
     5341 gggctttaac tactacggca ccgtcggctc gaaccgcgac gaattcatca tgatccgcaa
     5401 gatgaagaac gttaactggc tggatgatga aggtcgcgat caggtacagg aggcgaaaaa
     5461 atgaaaatac gttcgcaagt cggcatggtg cttaacctcg ataaatgtat cggctgccat
     5521 acctgttcgg tgacctgtaa caacgtctgg accggacgcg aaggcatgga gtacgcatgg
     5581 tttaacaacg tcgaaaccaa accgggcatt ggttatccga aaaactggga agatcaggaa
     5641 gagtggcaag gcggctgggt ccgcgatgtg aatggcaaga tccgtccgcg tctgggcagc
     5701 aagatgggcg taataaccaa aatcttcgcc aacccggtgg tgccgcagca gattgatgat
     5761 tactacgaac ctttcacctt cgactacgaa catttgcata gcacaccgga aggcaaacat
     5821 atccctactg ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa agtgatctgg
     5881 ggcccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5941 ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa acaccttcat gatgtacctg
     6001 ccgcgcctgt gcgaacactg cctcaatccc agctgcgtgg cgacctgccc aagcggcgct
     6061 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     6121 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     6181 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     6241 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatc
     6301 gaggaagcgt cgagcaccga gcgcgaagtt gacct
//
LOCUS       DAJVRP010000157         6335 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVRP010000157 DAJVRP010000000
VERSION     DAJVRP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.157"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(53..532)
                     /gene="xdhC"
                     /locus_tag="OQN12_003759"
     CDS             complement(53..532)
                     /gene="xdhC"
                     /locus_tag="OQN12_003759"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS1655926.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="OQN12_003760"
     CDS             complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="OQN12_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS1655927.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="OQN12_003761"
     CDS             complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="OQN12_003761"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS1655928.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4130..4885
                     /locus_tag="OQN12_003762"
     CDS             4130..4885
                     /locus_tag="OQN12_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1655929.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4964..5037
                     /locus_tag="OQN12_003763"
     tRNA            4964..5037
                     /locus_tag="OQN12_003763"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4996..4998,aa:Gly,seq:ccc)
     gene            5152..>6335
                     /locus_tag="OQN12_003764"
     CDS             5152..>6335
                     /locus_tag="OQN12_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1655930.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DS"
BASE COUNT         1584 a         1586 c         1639 g         1526 t
ORIGIN      
        1 gttaaacaca taacctcagt gaatccggcc tgaattcagg ccggatatcc ttttacttcg
       61 ttttctcgca atccagaact gtatttacaa tcatctgata ccccgtgcag cgacaaagat
      121 ttcccgccag tccgcgacga atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca
      181 gcattgccgt ggtagccata atcaggccag gcgtacaaaa cccgcactgc actgtgccgg
      241 atttcgcata agcctgctga acatgagaaa gttttccgcc tttcgcttca ccttccagcg
      301 tgcggatctc ttttccttca gcccaggcgg caaggtataa gcaactgtct attgctgtgc
      361 cgtcgaccaa caccgtacag gcaccacatt cacccacgca gcacccttgt ttgacactta
      421 gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc tggtgcggcg tgaagctgaa
      481 aaggcatccc gttaatggtg cattcggtgg taattgtttc gctgtgattc attgcaattt
      541 tccccccgcc gcggcgacgg cttcgctaat cacttttttg gtcatcgtct ggatgagatg
      601 cagacgaaac tctttactgg cccgccatga agaacgcggg gcgacatctt gcaggacaga
      661 ttcgctgata gcttccagcg tttgcaggtt taatggcgca ttttgtgcag tctgttcggc
      721 atgttggcag cgaatcggcg ttggcgcggc aacaccaaat gccaggcgta attcgctgaa
      781 attgccgtta tccagtcggc aatgtgcggc gcagccaatc gttgaaatat ccattgcgtc
      841 gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct ttcggctgtg gcggaaaatg
      901 aaaggcgacg aggatttcgt catgctcaag agacactttg cccggcccgg tgtgaaagcc
      961 attaatcggg acgaaacgaa caccgcgcgg ggagtggatc tccagtttcg cgtcataaat
     1021 tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg caaatatttc caccgtaggt
     1081 agcgacgtta cggatctgcg gtccagcaat gaacgtggcc gcagcacata acgccgggag
     1141 atgacgttga gttataggat cttctattag ctgggtaaat gtcgttgcag agccgatacg
     1201 tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc gccagattat gaatatcaac
     1261 aatatggcga taacggtcat tgtgatggtg gagctgaatc agtacgtcag tgccaccggc
     1321 gagcagtttg gcctgcgggt tgtcagccag caggttgatg gcatcggcaa gggttgctgc
     1381 gcgatggtaa gaagcaaaat caaacatgat gttatcctca aatcaatcct gccagatgga
     1441 actcttcata taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca
     1501 tcttcaccgc gttacgaata gcagcggcaa caggaattat gggtggctca cccagtgact
     1561 tatgtccgta tgcggattgc ggctcattga tttcgacgaa cgcgctttcc agttgtggca
     1621 gatccggcat ggtcggcatt ttgtaatcca gcagattggg gttacggacc acgccgcttt
     1681 tagcatcgat gatcatctct tcaaatagcg cccagtcaat gcccattccc attccgccgt
     1741 gtacctgacc ttctgccagc agtggattaa gaatatgccc tgaatcatga acgttgagga
     1801 tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc
     1861 caaacgccgg tgggttagtg gtggttttga tggagctttc agcagagagc tgcccgccgc
     1921 gttcagggtg gtagaaagcg tccatcgcca aatcttttaa cgacattaac ggctcttccg
     1981 gtcgttcaac cagcacgata tggcctttta tcagggtcaa attcatcgct gactgatgta
     2041 gcatgactgc ggcgtgagcg atgattttct cttttaataa tagtgccgca ctgcgcagcg
     2101 caggcgcggc aacatagctc tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt
     2161 cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg aacccccacg gtttctgcca
     2221 ccatttgcga gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt
     2281 tgatggttcc atcctgattc atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc
     2341 aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa ttgccttgct
     2401 ggttctggca ttctgcacgg cgtttttccc attcaaagat tttccggcct ttttcaagac
     2461 actccggcaa ccctgcgctg caaatacgtt tgcccgtgag cggattagca tctccttcgc
     2521 gggcggcgtt gcgtaaacga atttcaacag gatcaatacc taacgctgtc gcggcgtcat
     2581 caagcataga ctcaacggca aatacgactt gtggcgcgcc ataaccacgc atcgcaccag
     2641 ccgaggggag gttggtatag caggtctttg aactgtaagc gtaggcacaa cgaggataaa
     2701 ggtaagcgac tttattcccc cccgcagaag cgatggagtg cccgtgagat gcataagcgc
     2761 cggtgttaga cagaacatcc agactataac ctttcaaggt tccgtcgcgg ttcacgccca
     2821 tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac tcttcacggc
     2881 taagggaaac tttcaccgga atgccgccaa gcttgctggt caggaatgcc gccattggct
     2941 cttccagtac atcctgttta ttaccaaaac cgccaccgac aaacggtttg atgactcgta
     3001 cgcatgacca gggaatatcc agcgcctgac caaccacgcg gcgaacaatg tgcgggatct
     3061 gggtgctgga aacgatggta attcgcgagt catcctccat ccacgccagc gatgttacgc
     3121 tttccatgtg acaatgttga ataacgggag tctgatagtg cccctgtacc tggtagtcgg
     3181 cggcatcgat tgtttgttgg acattgcccg tcgacatcgt gctttgtttc agtaaattgc
     3241 cgccgttatg gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg gtgataacgg
     3301 gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc
     3361 gggccacgac gatggcaacg gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc
     3421 gatcggcggt atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct gtagcgaatg
     3481 gaatatcagg cacatcctcc caagtaaaaa tcgccagtac gcccggtaaa cttctggctt
     3541 gttcatcatt aatacttacg gcataaccat gtgcgatagg gctacgtaca tatttcgcgt
     3601 aacacatgcc cgccataaca taatcgtcag tatatcgtgc ccgcccggtg accttagcaa
     3661 tggcatcgac gcgcatgcat gattcacccg tagcggttgc ttcccgcgct tccatagaaa
     3721 tcccctcgat ttgaacattt ggttcttatg gcaaagattt agtctaagca ggcaagattt
     3781 acgccagaaa cgacgataag cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca
     3841 cagatatgat acgaaacagg actctgaata gggacagtga tacgtatcaa tatgagtggc
     3901 gataaaatca atgtgataaa tcaggattat tacgcatcag gatgggaaat agggtaaaaa
     3961 gtcaggaact tcgatacctc tacagcgaat attttgctga tgatacttcc ctttgccaaa
     4021 tgaaattaac ctctttctct attaccacgt ttttccagaa gcaagagatt gggtatcaag
     4081 aggctggctg ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg
     4141 acgcctgaat aaaaaatctc tgggtatcgt gatgttttta tcggttggac tgctattggc
     4201 gggctgttcg ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt
     4261 gaaacggggg gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct
     4321 ggcgcgactg aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact
     4381 gggtggggcg aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgactaaaac
     4441 cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg
     4501 ttgttggtta tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg
     4561 caataaaggg attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg
     4621 aaaggtggtg tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca
     4681 cagtgaagat tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca
     4741 aagcgtgaag gctgggcaaa aaatcgccac tatggggagc acggatgcgg catctgttcg
     4801 cctgcatttc cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc
     4861 gcagggcagc aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt
     4921 gctaaggaga gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg
     4981 gtagaacgag agcttcccaa gctctatacg agggttcgat tcccttcgtc cgctccatga
     5041 cctttcttaa agtactccaa cctactgaat cctaagggat atggctttaa gcctccttgc
     5101 agagttactt actgtaaccc actgtgtaac cctctcaggg catacagaca tatgattcag
     5161 ctatccagta gtcacaaact tccagcagtc tactatctct accagcgtaa cggcgtctac
     5221 tacttccgtc ttcgggtcag acaatcaaac aatgatagaa tgacctcagt atcattacgg
     5281 accaaggata gacgcaccgc tatggctcac tcaaggcaca tcaaggcagc actcaaggca
     5341 atacacgcag acagacccaa cgctacctat gaggagatga gggagcacct taaggacatc
     5401 gctgagtgtg aactgtctat gggccgttca gatctctttg agcctgacat gagggatatc
     5461 taccgggacc aatacggaga acttggtgag agccttaccg atgctctggc tagtgagccg
     5521 ctaagtattg accagcatcg ttacatcaat gaggccctga aggtgctaaa agcctgtatg
     5581 aggcgtatcg aagcgggaga tagtcagcct ctgatagatt acgtcgatcg ttttaatgat
     5641 attgatcggc aagatgacca agcagactct gtgtccctat ctgttagtgc tccagaagta
     5701 aaaccagaag ttacgcctag cattaccata gcgagcctct ttgagcaata cgaagcggag
     5761 aactctcaga actggaagcc tgcaacctta agggaaaaca aagcgtcaca cgctgcgtta
     5821 atcgaaatat tcgaccatct gggtctcaat gcagacgcta acagggctga catgcttaag
     5881 gtccgtgatg tgctccagca gttacctaag aaccgtaagc agcgctttaa ggatgcccca
     5941 cttgctgacc tgttgagccg tgaggataag acagattgtc tggatgtcgt taccatcaac
     6001 aacaagtatc ttattaagat ggctgctgtc tttaggtggg ctgtgcgcaa tgacttgatt
     6061 aaaaagaaca tgactgaggg tctggagctt aaagtccctc agcgtaaagc ctcaggggcc
     6121 cgtaacgcct tctctacgga gcaggtgggg caactactgg tcgcagctaa ggcatactct
     6181 cagaagacct ctggtaagcc ctaccactat tacgttacgg ctttggctgc tatcactggg
     6241 gcgagactca atgaaatagc ccaactacag gtcaaagacg tcaggaccac tgaggcggga
     6301 accgtttata ttcatatcaa tgaagacgat agtag
//
LOCUS       DAJVRP010000158         6331 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVRP010000158 DAJVRP010000000
VERSION     DAJVRP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.158"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            5..436
                     /locus_tag="OQN12_003765"
     CDS             5..436
                     /locus_tag="OQN12_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCS1655931.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OQN12_003766"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="OQN12_003766"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1655932.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OQN12_003767"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="OQN12_003767"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1655933.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OQN12_003768"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="OQN12_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1655934.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="OQN12_003769"
     CDS             complement(4438..5145)
                     /locus_tag="OQN12_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1655935.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OQN12_003770"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="OQN12_003770"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1655936.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="OQN12_003771"
     CDS             complement(6003..6206)
                     /locus_tag="OQN12_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1655937.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1405 a         1810 c         1648 g         1468 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc caccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct t
//
LOCUS       DAJVRP010000159         6290 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVRP010000159 DAJVRP010000000
VERSION     DAJVRP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.159"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..1017
                     /locus_tag="OQN12_003772"
     CDS             <1..1017
                     /locus_tag="OQN12_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1655938.1"
                     /translation="SQIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGV
                     PVMLIGTPKAREIFEADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQ
                     LLQRKDALLSDEVRDVWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQD
                     ELKPVHPMLEALRSGIPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQEL
                     DTEDQRHLYLMLKEDYDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSEL
                     EKPSKSKKVSAIKVVKPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLF
                     KQAG"
     gene            1020..2546
                     /locus_tag="OQN12_003773"
     CDS             1020..2546
                     /locus_tag="OQN12_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCS1655939.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            2547..4163
                     /locus_tag="OQN12_003774"
     CDS             2547..4163
                     /locus_tag="OQN12_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCS1655940.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(4219..4371)
                     /locus_tag="OQN12_003775"
                     /pseudo
     CDS             complement(4219..4371)
                     /locus_tag="OQN12_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4394..4771)
                     /locus_tag="OQN12_003776"
     CDS             complement(4394..4771)
                     /locus_tag="OQN12_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655941.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(5181..5552)
                     /locus_tag="OQN12_003777"
     CDS             complement(5181..5552)
                     /locus_tag="OQN12_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655942.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(5613..6110)
                     /locus_tag="OQN12_003778"
     CDS             complement(5613..6110)
                     /locus_tag="OQN12_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655943.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(6186..>6290)
                     /locus_tag="OQN12_003779"
     CDS             complement(6186..>6290)
                     /locus_tag="OQN12_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'') family aminoglycoside
                     nucleotidyltransferase"
                     /protein_id="HCS1655944.1"
                     /translation="QAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1736 a         1310 c         1551 g         1693 t
ORIGIN      
        1 tcgcaaatag ccaatgcaca tgctttaggg ttgttggtta ttgatgaaat tcagcattta
       61 agccgctctc gttcgggtgg atctcaagag atgctgaact tttttgtgac gatggtgaat
      121 attattggcg taccagtgat gttgattggt acccctaaag cacgagagat ttttgaggct
      181 gatttgcggt ctgcacgtag aggggcaggg tttggagcta tattctggga tcctatacaa
      241 caaacgcaac gtggaaagcc caatcaagag tggatcgctt ttacggataa tctttggcaa
      301 ttacagcttt tacaacgcaa agatgcgctg ttatcggatg aggtccgtga tgtgtggtat
      361 gagctaagcc aaggagtgat ggacattgta gtaaaacttt ttgtactcgc tcagctccgt
      421 gcgctagctt taggcaatga gcgtattacc gctggtttat tgcggcaagt gtatcaagat
      481 gagttaaagc ctgtgcaccc catgctagag gcattacgct cgggtatccc agaacgcatt
      541 gctcgttatt ctgatctagt cgttcccgag attgataaac ggttaatcca acttcagcta
      601 gatatcgcag cgatacaaga acaaacacca gaagaaaaag cccttcaaga gttagatacc
      661 gaagatcagc gtcatttata tctgatgctg aaagaggatt acgattcaag cctgttaatt
      721 cccactatta aaaaagcgtt tagccagaat ccaacgatga caagacaaaa gttactgcct
      781 cttgttttgc agtggttgat ggaaggcgaa acggtagtgt cagaactaga aaagccctcc
      841 aagagtaaaa aggtttcggc tataaaggta gtcaagccca gcgactggga tagcttgcct
      901 gatacggatt tacgttatat ctattcacaa cgccaacctg aaaaaaccat gcatgaacgg
      961 ttaaaaggga aaggggtaat agtggatatg gcgagcttat ttaaacaagc aggttagcca
     1021 tgagaaactt tcctgttccg tactcgaatg agctgattta tagcactatt gcacgggcag
     1081 gcgtttatca agggattgtt agtcctaagc agctgttgga tgaggtgtat ggcaaccgca
     1141 aggtggtcgc taccttaggt ctgccctcgc atttaggtgt gatagcaaga catctacatc
     1201 aaacaggacg ttacgctgtt cagcagctta tttatgagca taccttattc cctttatatg
     1261 ctccgtttgt aggcaaggag cgccgagacg aagctattcg gttaatggag taccaagcgc
     1321 aaggtgcggt gcatttaatg ctaggagtcg ctgcttctag agttaagagc gataaccgct
     1381 ttagatactg ccctgattgc gttgctcttc agctaaatag gtatggggaa gccttttggc
     1441 aacgagattg gtatttgccc gctttgccat attgtccaaa acacggtgct ttagtcttct
     1501 ttgatagagc tgtagatgat caccgacatc aattttgggc tttgggtcat actgagctgc
     1561 tttcagacta ccccaaagac tccctatctc aattaacagc actagctgct tatatagccc
     1621 ctctgttaga tgctccacga gcgcaagagc tttccccaag ccttgagcag tggacgctgt
     1681 tttatcagcg cttagcgcag gatctagggc taaccaaaag caagcacatt cgtcatgact
     1741 tggtggcgga gagagtgagg cagactttta gtgatgaggc actagagaaa ctggatttaa
     1801 agttggcaga gaacaaggac acgtgttggc tgaaaagtat attccgtaag catagaaaag
     1861 cctttagtta tttacagcat agtattgtgt ggcaagcctt attgccaaaa ctaacggtta
     1921 tagaagcgct acagcaggca agtgctctta ctgagcactc tataacgaca agacctgtta
     1981 gccagtctgt gcaacctaac tctgaagatt tatctgttaa gcataaagac tggcagcaac
     2041 tagtgcataa ataccaagga attaaggcgg caagacagtc tttagagggt ggggtgctat
     2101 acgcttggct ttaccgacat gacagggatt ggctagttca ctggaatcaa cagcatcaac
     2161 aagagcgtct ggcacccgcc cctagagttg attggaacca aagagatcga attgctgtac
     2221 gacaactatt aagaatcata aagcgtctag atagtagcct tgatcaccca agagcgacat
     2281 cgagctggct gttaaagcaa actcctaacg gaacctctct tgcaaaaaat ctacagaaac
     2341 tgcctttggt agcgctttgc ttaaagcgtt actcagagag tgtggaagat tatcaaatta
     2401 gacggattag ccaagctttt attaagctta aacaggaaga tgttgagctt aggcgctggc
     2461 gattattaag aagtgcaacg ttatctaaag agcggataac tgaggaagca caaagattct
     2521 tggaaatggt ttatggggaa gagtgagtgg ttaggctagc tacatttaat gacaatgtgc
     2581 aggttgtaca tattggtcat ttattccgta actcgggtca taaggagtgg cgtatttttg
     2641 tttggtttaa tccaatgcaa gaacggaaat ggactcgatt tactcatttg cctttattaa
     2701 gtcgagctaa ggtggttaac agtacaacaa agcaaataaa taaggcggat cgtgtgattg
     2761 agtttgaagc atcggatctt caacgagcca aaataatcga ttttcctaat ctctcgtcct
     2821 ttgcttccgt acgcaacaag gatggagcgc agagttcatt tatttacgaa gctgaaacac
     2881 catatagcaa gactcgttat cacatcccac agttagagct agctcggtca ttatttttaa
     2941 ttaactccta tttctgtcga agctgtttga gcagtaccgc tttacagcaa gagttcgacg
     3001 ttcagtatga ggttgagcga gatcatttag agataaggat cttacccagt tcatcgtttc
     3061 ctaaaggggc gttagagcag tcggccgtag tgcagctttt ggtttggttg ttttcggatc
     3121 aagatgttat ggattcgtat gaaagtattt ttaggcacta tcaacaaaat agagaaatta
     3181 agaacggcgt tgaaagctgg tgctttagct ttgaccctcc gcccatgcag ggttggaaat
     3241 tacatgtaaa aggacgttct tctaacgagg ataaggatta tttagttgag gaaatagtag
     3301 gtttagaaat caacgctatg cttcctagca caacagctat tagccatgcc tcttttcagg
     3361 aaaaggaggc aggtgatggt agtacgcagc acatagcggt ttcaacagag tcagttgttg
     3421 atgatgagca tctacagttg gacgatgagg aaacagccaa tatagacaca gacacacgag
     3481 tcatagaggc tgagccgaca tggataagtt ttagtagacc tagtcgaatt gaaaaatctc
     3541 gcagggcaag aaaaagtagc caaactattt tagaaaaaga agaagcaaca acaagtgaaa
     3601 atagtaattt ggttagtact gatgagccac acttaggtgg tgtcctagca gcggcagatg
     3661 tgggtgggaa gcaggatgca accaattaca actctatttt tgctaatcga tttgctgctt
     3721 ttgatgagct actttcaatt ctaaaaacta aatttgcatg tcgggtgctt tttgaagaaa
     3781 ccttggtttt gccaaaagtt gggcgtagcc gattacatct gtgtaaagat ggctcaccaa
     3841 gagtgattaa agccgttggg gtgcaacgta atggcagtga atttgtattg ctagaggtgg
     3901 atgcatcgga tggggtgaaa atgctttcta ccaaagtgtt gagtggcgtt gatagcgaaa
     3961 catggcggaa tgattttgaa aagatacggc gtggagtggt gaagagctca ttgaattggc
     4021 caaatagttt gtttgatcaa ttatatggac aagacgggca tagaggggtg aatcatccaa
     4081 aggggttggg ggagctgcaa gtatcgagag aggatatgga agggtgggct gagagagtgg
     4141 ttagagagca atttacgcat taaaggaatg actgaaagag cctgtaaacc cttttgtgta
     4201 agtgcttttg ccggtcagtt aaaggtggcc atttaaacgg tcaccaaatt cgatcataaa
     4261 acggttcatg gccggcttcc agttgcggat cggcatcgtc catttcttgg tcgccgcctg
     4321 gatagccagg tacaccacct tcatcgctga ttcgtccgta gggaacacct tgagttcata
     4381 ttaatggaat tttctacaaa tagcctccgt ggttttgagg ggggattaca gacgatccat
     4441 agtagtaatc caatgagttc ttgagcgcgg cgacatgttt ggacgccttg gcaaaaatta
     4501 gagcctgctt gaagtgcagg cgagcacgtg cttggacgat tgatccattc gcggtcaaaa
     4561 actcaatctt ggatgacagc gtgtcaggaa atccaatatc gaagtcccgc cccgtgtcaa
     4621 agtagggcat ttcatgatca aaggacgaag cttcccaggc cacttcccta atattccagc
     4681 cgctagaaac aagatttcgt acgacttcat caactttttt gctttccaac ttgtcacaaa
     4741 gtgcataggc aagagctttg gcggcagaca cgtgcgtttc ctcttaaaca taggtcgaac
     4801 ggatacccga ggcaaagcgg agcattagtc ccaaagcatc tccggtagat ccggaggctc
     4861 catactgaag gcatggcatc ttgggcattg atatttgaaa cttctctcta gatagcaggg
     4921 atttcttggc cttgcctcat gctgatagct tgtaaatatc acacgatcct tagccccaca
     4981 aagtgagcat actccgtagc tttcatttat agaaatcgtc gttagattgt cgcagcatgt
     5041 gcacgttccg actatttgct caggaagcat cgccatacct tggcctaggt aaagaagatc
     5101 agactcgtag ccacatcggt ggcaactgag cttgtacgca atgcccatct ggttgtctca
     5161 cctgaatcgt aacgtcagag ctattcatcc atccattcgg catcaatgta tgccactaag
     5221 ccgaccagct gaccgtttgg actgaagtat gttgcctgca ccccacttga catctcatca
     5281 ataccaccgc actgcgctag gcgccggtcc cacttgccca cagaagcgtc aggatcggct
     5341 gagttcccca agacccttgt gagccatgcc tcctttaact caacctctcg aatgattgcg
     5401 tcggccagct cctccgcgtt atctagttcg acatagtctg gaattcggac gtacgttctg
     5461 aacacagctg gatacttact tgggcgattg tcatacatga catcaacaat gtacccgttt
     5521 gtgtaaccgt ctcttggagg ttcgtatgac actagtggtt cccctcagct tgcgactaga
     5581 tgttgaggcc taacatttta ttagagagca ggctagttgc ttagatacat gatcttcagg
     5641 ccgttatctg tcagggcaag cgaaaattgg ccatttatga cgaccaatgc cccgcagaag
     5701 ctcccatctt tgccgccata gacgccgcgc cccccttttg gggtgtagaa catccttttg
     5761 ccagatgtgg aaaagaagtt cgttgtccca ttgttggcaa tgacgtagta gccggcgaaa
     5821 gtgcgagacc catttgcgct atatataagc ctacgatttc cgttgcgact attgtcgtaa
     5881 ttggatgaac tattatcgta gttgctctca gagttgtcgt aatttgatgg actattgtcg
     5941 taattgctta tggagttgtc gtagttgctt ggagaaatgt cgtagttgga tggggagtag
     6001 tcatagggaa gacgagcttc atccactaaa acaattggca ggtcagcaag tgcctgcccc
     6061 gatgccatcg caagtacgag gcttagaacc accttcaaca gatcgcgcat agtcttcccc
     6121 agctctctaa cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga acgaattgtt
     6181 agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtgaac aaattcttcc
     6241 aactgatctg cgcgcgaggc caagcgatct tcttgtccaa gataagcctg
//
LOCUS       DAJVRP010000160         6259 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVRP010000160 DAJVRP010000000
VERSION     DAJVRP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.160"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..667)
                     /locus_tag="OQN12_003780"
     CDS             complement(<1..667)
                     /locus_tag="OQN12_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCS1655945.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(657..2414)
                     /locus_tag="OQN12_003781"
     CDS             complement(657..2414)
                     /locus_tag="OQN12_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCS1655946.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2430..3314)
                     /locus_tag="OQN12_003782"
     CDS             complement(2430..3314)
                     /locus_tag="OQN12_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1655947.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3325..3930)
                     /locus_tag="OQN12_003783"
     CDS             complement(3325..3930)
                     /locus_tag="OQN12_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1655948.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4101..>6259)
                     /locus_tag="OQN12_003784"
     CDS             complement(4101..>6259)
                     /locus_tag="OQN12_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCS1655949.1"
                     /translation="EQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGN
                     LTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIK
                     GSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLIND
                     EDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIA
                     TFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPV
                     VLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYK
                     DAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMK
                     HHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYF
                     CCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKA
                     DSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVLK
                     ELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWS
                     TLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLL
                     ALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1836 a         1567 c         1314 g         1542 t
ORIGIN      
        1 atggcagtgc tattttgatt cgacgttgat caagtttctg ataattccaa cgacggtcga
       61 cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat
      121 gatgctgcca tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccacagactt
      181 tacgccacct cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa
      241 tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc
      301 atcctgtcac cccactcact tcaggacgga taaaactaaa cttcagcggg tagagaaaaa
      361 aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg cgttgttcga
      421 atgtcgagct acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa
      481 caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctggggatat
      541 catggctgtt aaggtcctga accgcggtga actgattaag agatccatag gtgaagaaga
      601 aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc cgacgttcag
      661 ttatcacgac gcaccgccat cgaaaccgta aaaatgcccc acacatcaat ccgttgagtg
      721 catttatcaa atccggcatt acgcacgagc gaatccatct ccccttgcga acgcacgcgc
      781 attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt
      841 gggtgccacg gctgcccggt gtagatgaga atgccacccg gttcgatggc attggctaga
      901 cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatatag gccagagaca
      961 atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc
     1021 tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca
     1081 acatttaact cactgtaatc acgtaacaaa atatcgctta cggcaggctc gtttgccagc
     1141 gcatccagta catagcgtcc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct
     1201 ttggcgtgga gatcggcaac ggcttgttta atcaatattt gcaaatgggt tttgcgctgg
     1261 cgaataccgc gccatccaac actgttcagg tagattttgt cgattaaacg cccgaatgcg
     1321 ttactgcctt gcggttgatt ttgatagaca taatccaggg aactgcctga atcaaagccg
     1381 gtactcattc cgagatgcag gcccgcagag tgcgcaccga acaatttcat cgcctttcgc
     1441 ataaagcgat acgctaaatc aaccggcgat aatggcacgg gtccaccaga aaggagccgc
     1501 catcgatccg ctgatggtcc tgtgcggtct tcatgctgat aatcaaattt ttgcgatttg
     1561 ttagcatata agcggcagat aaagctttgc attttttcaa atgcctgcgc cctgttctct
     1621 tcacccaatg tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt
     1681 aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc acctgatatc
     1741 agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc agacgtttta
     1801 tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa
     1861 ctcgccaccc gttgccgatc gtgggtcaaa tagcgccctt tcacatagga attaataaaa
     1921 aatagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg cacatacaat
     1981 ttaaccttaa aggccggaga agccagcacc agcccacgaa ttgcaggtgc ataatcatga
     2041 acccatgtgg caacaagcac tgcgccgacg ctttgcgcga tcactaccac ctcttccagt
     2101 ccgacctggc tgtcgctgac agcaaaacgg acaaattcat cgacatcctg cactgagcgc
     2161 ataagagacg ggctataacc acgcggcccc gaattttttc catgcccccg ggcatcccag
     2221 gcataaaaag cagtatcagg catcgccagt tcatcaacga gatgttgtag acgaccagaa
     2281 tgttcatgcc cgcgatgaaa taagacgatg acctttttcg cccctggctg taaagtgggc
     2341 cagtgccgat aatacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagggatg
     2401 cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat aaaataaaaa
     2461 aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc atgtcctagt
     2521 aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat cactacatcg
     2581 ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa tgtatttagc
     2641 ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgattac gccccccagc
     2701 agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat
     2761 ttcccccata aatactgtgc aatatcgttt gactcggtta acgccaaaag aaataacacc
     2821 agtaacgccc cgccctggat atttatggtt ggcaacatta atagccaggc ggcatgactc
     2881 aaggcaaaaa cggtcattat ccatccccaa aaaatggcgc tcacggtatg ctgaaaaccg
     2941 gaggggtcac ccacaaaaac tcgccaggtg gctaatatca gaaacccagt caaaggtata
     3001 aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc
     3061 caatataacc aacgcgaaaa atgtacggat ataagcgtac aatattcttt caatgccaga
     3121 aaacttatca gcgtaaaaaa cgtcaacgtc atccagcgtg ggcctgaaat caccagtgaa
     3181 aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacttcatc cccttttctc
     3241 tcaggtaacc gccatagcag aaaacggttt atcagcttgc taaaattaac aacgcgaaaa
     3301 gtgttgccag agatttttcc agcattatct ttcagcgctc ataagaagca cactccgaca
     3361 acgattaatc gcagtacaga taaacagaat tgaagcaatg ctccataaaa gattattcca
     3421 ttgcatccac tgtggataaa tggcaacggc cagaccccac agaccaaata ttaacgcgcg
     3481 atcacttttg ccaaatggac cggcataact gcgaacaccg ttaatcgtct gcgcgagtaa
     3541 accgcaaaac tcggtcaata tggtgcaaaa taacatgagt atcacgagtg atgcgttact
     3601 ttccggtaaa aataaaaacg gtaaatagag cgcaatatcg gaaataacat cgccagtctc
     3661 attcaaaatc gcccccagac gtgtttgctg attgcactca cgcgccaaca tgccatccag
     3721 cgcattgagc gccatacgga taaaaagcac gatgggcaat agcaaaaaga ggatgggttg
     3781 tgccaccaac accaacagca atccggtaaa aagagaaagc gccagtgccg taagagtgat
     3841 gtgattcgct gtaacgtggt gcttataaag ccaaaacatc gtcggcctta acagcgactg
     3901 aaagagcggt tttatctggt agagcgtcat tatcaatcct tgataattcg gtttgcgcta
     3961 tggtgcatca attcccctga cggagatatc gcaacacgcc gcttcattaa aaattcagaa
     4021 tatatcaagg tattgggggc tcactgtcag gaatgattat ccccaataaa tcaagtagtt
     4081 aaaaattatt acgctgttat tcaatacaac tcatcaagtt tcctcatcgc ttgttcccgt
     4141 attgcgcgcg tcttaccgtt aagcgccagc tggcggacta aatccagttc agggttaaca
     4201 agctctaacg ccagcaaagg atcacgtttc agcctcgcct tcaatacatc aactggaaag
     4261 cggggattat tcatcgccac aatccaccac tcgggatcaa tttctgccgc cagtgccgca
     4321 gagggggtgt ggcggttgct aagtgcgccg aacttgaaaa atgtgagatt gcttttcgcc
     4381 agtgtactcc accatatacg atccggttgt tttgccagta catccagttg tgctaacgca
     4441 tcggggtcga gtgcataata aacacgtaat gcttcatcaa cctgcgcacg ggagctattt
     4501 tcctgcctca gaaaaaactt cgcccaaata tcattgcgtg gcgtgttttg ctgtaattcc
     4561 ttgagaacaa cagcgggtaa attatcccaa ctaactaatg cactctctaa aaagtcccag
     4621 tcttccaaac gtatcagggc ggcggcattt aatagcatct ggtcatcaac ttcgccgtgg
     4681 ctaatcagct cagacaaaat catatcagcg ctggtttcat atgcatctga atctgctttc
     4741 ggattatttt tccgatgcga taaagcacgt tgccaaattt tttgtttcat cgacgtgctc
     4801 agatactgat ttttcgccag attactccac ccctcaggat cgttgaaacg tgtcaataac
     4861 cgttgtaaag cagcgtcaga gtgaattacc tgcatatccc gacagcaaaa gtaggcaggg
     4921 gattttttct ccatcctttt aacaaaatta aactgtaagt tatctggcaa tgctaaaaat
     4981 aacacacgta ttagatcatc gtcattctcg aatttttggt ataaataggc tgcaactttg
     5041 tcactctctt gtggcgtcat ccgcaataat tgaccatggt gtttcatctc tgccagcttg
     5101 aacgctaagc ttgtgacaac attgtttgat gaatcgttcg ccaactgcat tgcctgattg
     5161 taagtgagtt gtcctccagc accgacctga cgaatagagg aatcatcact gcggatatag
     5221 ccatcaataa tggattcggg caataaccac gcatctttat aaagctcctg aatgatcata
     5281 tcccaatctc ttttcgccag agtcatgacg atatccagag ggagattttc acgtagcgca
     5341 agattcaacg gaccgacacc atcaggtaga cgactcattc gcgtcattat ttcgacagag
     5401 agcggttcac ggtcgagcaa ccccagaaca acaggctggt aacccaattg ctgatcggtc
     5461 aactccagaa tctcattgtc aggcatatat ctcgccatac tttcaatcgt ttgttcatca
     5521 ttgctattaa taagttgttt gcgtaggtta gctgaaattc ctttaagcct tgggagagtt
     5581 cgcattatgg caagatcaaa tgtcgcaata tctcgtcctt caaatgcagc aacgttgcct
     5641 tcatccagca tttgcaattc ctgcactaac tcaggagaag gcttagggcg aaacagcgct
     5701 tcttttttca tctgattaca gacattccct gccatgcgaa tattgcttcg cttaaaaatt
     5761 tctttcagag catcttcatc atttattaga ccccaataat atgcatcagg atttattatc
     5821 atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac catgaattca
     5881 ctatttgtcg aagacgcaac aacaggtgca attatgtcat tgataaatac cccacgcgca
     5941 ttaatactcc ccttaatcgt agacggatta tcgtaatatg agtcgataaa taccagtcca
     6001 cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt cgttgccgtc
     6061 acgttaccga gaataaccca tggattagaa agagagaaag tggaaatatt acctttgact
     6121 gtcaaattgc caagaacaat caatttttga ttactctcta tattaccttc taccactaaa
     6181 tcgccacgat gcactttcca gtccgatgca gataaaccga aggggacaag attcacgcta
     6241 ctgttgatag cttgttcaa
//
LOCUS       DAJVRP010000161         6254 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVRP010000161 DAJVRP010000000
VERSION     DAJVRP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.161"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OQN12_003785"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OQN12_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1655950.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OQN12_003786"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OQN12_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1655951.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OQN12_003787"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OQN12_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="OQN12_003788"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="OQN12_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1657 a         1456 c         1477 g         1664 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atga
//
LOCUS       DAJVRP010000162         6212 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVRP010000162 DAJVRP010000000
VERSION     DAJVRP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.162"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            73..378
                     /locus_tag="OQN12_003789"
     CDS             73..378
                     /locus_tag="OQN12_003789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1655952.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="OQN12_003790"
     CDS             complement(454..918)
                     /locus_tag="OQN12_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1655953.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OQN12_003791"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OQN12_003791"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1655954.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="OQN12_003792"
     CDS             complement(1787..2476)
                     /locus_tag="OQN12_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1655955.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OQN12_003793"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OQN12_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1655956.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OQN12_003794"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OQN12_003794"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1655957.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OQN12_003795"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OQN12_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1655958.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1575 a         1688 c         1548 g         1401 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct caccgacaat taaaaacacg ac
//
LOCUS       DAJVRP010000163         6200 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVRP010000163 DAJVRP010000000
VERSION     DAJVRP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.163"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OQN12_003796"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OQN12_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1655959.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OQN12_003797"
     CDS             2089..2238
                     /locus_tag="OQN12_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655960.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OQN12_003798"
     CDS             complement(2983..3423)
                     /locus_tag="OQN12_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1655961.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OQN12_003799"
     CDS             3641..4030
                     /locus_tag="OQN12_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655962.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OQN12_003800"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OQN12_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OQN12_003801"
     CDS             complement(4585..5421)
                     /locus_tag="OQN12_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1655963.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="OQN12_003802"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OQN12_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1655964.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1431 a         1618 c         1642 g         1509 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aacaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJVRP010000164         6057 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVRP010000164 DAJVRP010000000
VERSION     DAJVRP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.164"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            381..1409
                     /gene="torT"
                     /locus_tag="OQN12_003803"
     CDS             381..1409
                     /gene="torT"
                     /locus_tag="OQN12_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS1655965.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1382..2074)
                     /gene="torR"
                     /locus_tag="OQN12_003804"
     CDS             complement(1382..2074)
                     /gene="torR"
                     /locus_tag="OQN12_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS1655966.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2161..3333
                     /gene="torC"
                     /locus_tag="OQN12_003805"
     CDS             2161..3333
                     /gene="torC"
                     /locus_tag="OQN12_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS1655967.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3333..5873
                     /gene="torA"
                     /locus_tag="OQN12_003806"
     CDS             3333..5873
                     /gene="torA"
                     /locus_tag="OQN12_003806"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCS1655968.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5870..>6057
                     /gene="torD"
                     /locus_tag="OQN12_003807"
     CDS             5870..>6057
                     /gene="torD"
                     /locus_tag="OQN12_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCS1655969.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1472 a         1516 c         1678 g         1391 t
ORIGIN      
        1 ccatttcggt cccagttagg acgctttctc atcaagccag tccgcccacc actgcatcat
       61 ctccctacgc ttatcgaaat actgagcatg gttgtaaatt caattaaccc agtgcgttga
      121 caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta atgctctgtt acaggtcact
      181 aataccatct aagtggttga ttcatagtga ctggatatgt tgtgttttgt cgcgtttttc
      241 agtctattat ttaatcatta agtaatctaa tgctatgatt tttaaattat ttatgtgatt
      301 ttgtcatcct ttaggtgaaa aaggttgagt cgcaaagtgg aatgcatcta gcataaagcc
      361 ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc cttttcatgt
      421 tgtcggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat ttcacggtgc
      481 aagcctctat gccgcttaaa gccaaacgcg tatggaaact gtgcgcgctt tatcccagcc
      541 tgaaagattc atactggtta tcgttgaact atggtatgca ggaggctgct cgccgctacg
      601 gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc cagcaagcac
      661 aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt agcacgacgt
      721 catttcccga cctgcaaaag caggtagcaa atctgccggt gatcgaactg gtaaatgctc
      781 ttgatgatcc ccaggtgaaa agccgcgttg gtgtaccctg gtttcagatg ggctatcaac
      841 ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact gaacgtgctg ctgatgcccg
      901 gacccgataa cgccgggggc agtaaggaga tggtcgaggg ttttcgcgca gccattgccg
      961 gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa atccagcgca
     1021 acctgttgca ggagatgctg gaacgccacc cagaaatcga cgtcgttgcc ggaacggcca
     1081 ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt accgtggtgt
     1141 cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg attatggctg
     1201 ccagcgatca aatggtctgg cagggggaac tggcagttga gcaggccatc aggcaattac
     1261 aggggcaatc ggtgtctgat aatgtcagcc caccgatttt agttctgacg ccgaaaaatg
     1321 ccgaccgaga acatatccgc cgctcgctgt caccgggggg atttcgtccg gtctattttt
     1381 atcagcacac atcagcggct aagaaataac cttcaccatg ttgcgtcacc agtaaatccg
     1441 cgctgagttt atgacgtaaa cgacgaatta acacatcgac ggtgcgcagg tcagggtttt
     1501 ccacccgacg cgcagaaagc atacgcagca gacgctcacg gctgagaatt tcgcccggat
     1561 tagtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc ggctcgccat
     1621 cccgctccag cgtatggcgc gacacattca ggcaataacc cgcaaagcga tagcagtagt
     1681 cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga tttttcaccc
     1741 gtactaccag ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg cccatttcca
     1801 gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc cccaccgttg
     1861 agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggtaag ttgatatcca
     1921 gcagaattaa atctaccggc tgattctgca taatttcccg tagcccggca ccgctcgccg
     1981 taacggaaac ggtatacccc tcctgagtga agtaggattg taatcgcgcc tgggtaaccg
     2041 gcttatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca ttctgttcat
     2101 atctgttcat atttgctcat taagatcgct tcactaaacc ataattctac aggggttatt
     2161 atgcggaaac tctggaacgc gctacgccga cccagtgctc gttggtcggt actggcgctg
     2221 gtcgcaattg ggattgtgat tggcattgcg ctgattgtat tgccacacgt tgggatcaaa
     2281 gtcaccagca caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt gtatgaagaa
     2341 tataaacagt cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg ccatgactgt
     2401 catatcccgc cggatattcc aggcatggtg aagcgcaaac tggaagcgag caacgacatt
     2461 taccagacct ttattgccca ctccattgat acacctgaaa aattcgaagc caaacgcgcg
     2521 gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca actcggcaac ctgtcgctcc
     2581 tgccataact acgatgcgat ggatcatgcc aggcagcatc ctgaagcggc acgtcagatg
     2641 aaggtggcag cgaaagataa tcaatcctgc atcgactgtc ataaaggtat tgcccaccag
     2701 ttaccggata tgagtagcgg cttccgtaag cagttcgatg agctgcgcgc cagtgctaat
     2761 gacagtggtg acacgctgta ctctattgat attaagccaa tttatgcggc gaaaggcgat
     2821 aaagaagcct ctggttctct gctgcctgct tcggaagtga aagtccttaa acgtgacggc
     2881 gactggctgc aaattgaaat taccggctgg acggaaagcg ccggacgtca gcgtgtactc
     2941 acccaattcc caggtaaacg catctttgtt gcctcgattc gtggtgatgt gcagcagcag
     3001 gtgaaaacgc tggagaaaac caccgttgcc gacaccaata ccgagtggag caagttgcag
     3061 gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata tcaaaccgat ctgggcctat
     3121 gcggattcgt tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga aatcgcccac
     3181 tttgacgcta acggttggat cggcacgctc aacggcatga ttggctttac cagtctcgat
     3241 aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga atgcgtctga caccgcaggt
     3301 aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa taacgatctc tttcaggcat
     3361 cacgtcggcg ttttctggca caacttggcg gcttaaccgt cgccgggatg ctggggccgt
     3421 cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc gactgaggct gtcatctcga
     3481 aagagggcat tcttaccggg tcgcactggg gggctatccg cgcgatggtg aaggatggtc
     3541 gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg atcgccggat
     3601 tgccggatca cgtacacaac gcggcacgta ttcgttatcc gatggtacgc gtggactggc
     3661 tgcgtaagcg ccatctgagc gacacctccc agcgcggtga taaccgtttt gtgcgcgtga
     3721 gctgggatga agccctcgac atgttctatg aagaactgga acgcgtacag aaaactcacg
     3781 ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac ggggatgttc cataacgctt
     3841 cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg ggcggagatt
     3901 actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt cggttcaatg gaagtgtatg
     3961 aacagcaaac ctcctggccg ctggtattgc agaacagcaa aaccattgtg ctgtggggct
     4021 ccgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat gtttatgaat
     4081 attacgagca gctaaaagcg aaagtcgccg ccggtgaagt tgaggtcatc agcatcgatc
     4141 cggttgtcac atccacccat gagtatctgg ggcgcgagca tgtgaagcac attgcggtta
     4201 acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca tacgctgtac agtgaaaacc
     4261 tgtacgacaa aaacttcctc gctaactact gtgtgggttt tgagcagttc ctgccgtatc
     4321 tgctgggtga gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa ctgaccggca
     4381 ttgatgccga aaccattcgt gggctggcgc ggcagatggc ggcgaacaga acgcaaatta
     4441 ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca gtgggcgtgg atgattgtgg
     4501 ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggttttggc tggcactaca
     4561 acggcgcagg cacgccgggg cgtaaaggcg ttattctgag tggtttctcc ggctctacgt
     4621 cgattccgcc tgttcacgac aacagtgact acaaaggcta cagcagcact attccgattg
     4681 cccgttttat cgatgcgatc ctcgaaccgg ggaaagtgat caactggaac ggtaaatcgg
     4741 taaaactgcc gccgctgaaa atgtgtattt ttgccggaac taacccattc catcgccatc
     4801 agcagatcaa ccgcattatt gaaggctggc gcaagctgga aacggttatc gccatagata
     4861 accagtggac ctcaacctgc cgctttgccg atatcgtgct gcctgcgacc acgcagtttg
     4921 agcgtaacga tctcgaccag tacggcaacc actccaaccg tggcattatc gccatgaaac
     4981 aggtggtgcc gccgcagttc gaggcgcgca acgactttga tattttccgc gagctgtgcc
     5041 gtcgctttaa tcgtgaagaa gcctttaccg aagggctgga cgaaatgggc tggctgaaac
     5101 gcatctggca ggaaggtgta caacaaggca aaggacgcgg cgttcatctg ccagcgtttg
     5161 atgacttctg gaataacaaa gagtacgtcg agtttgacca tccgcagatg tttgttcgcc
     5221 accaggcatt ccgcgaagat ccggatctcg aaccgctggg cacgccgagt ggcctgattg
     5281 agatctactc gaaaaccatc gccgatatga actacgacga ttgtcagggg catccgatgt
     5341 ggtttgagaa aatcgaacgc tcccacggtg ggcccggctc gcagaagtat ccgttgcatc
     5401 tgcaatctgt gcatccggat ttccgacttc actcgcagct atgtgagtcg gaaacgctgc
     5461 gtcagcaata tacggtagcg ggtaaagagc cagtgttcat taacccgcag gatgccagcg
     5521 cgcgcggtat tcgtaacggt gatgtggtac gcgtctttaa cgctcgcggt caggtgttgg
     5581 caggagcagt agtttctgac cgctatgcac ccggcgtggc gcgaattcac gaaggggcat
     5641 ggtacgatcc agataaaggc ggcgagcctg gtgcgctgtg caaatacggt aaccccaacg
     5701 tgctgaccat cgacatcggg acttcgcagc tggcgcaggc ggccagtgcg cacactacgc
     5761 tggtggaaat tgagaagtac aatggaacag tggagcaggt gacggcgttt aacggccccg
     5821 tggagatggt ggcgcagtgc gaatatgttc ccgcgtcgca ggtgaaatca tgaccacgct
     5881 gacagcacaa cagattgcct gtgtttacgc ctggctggcg cagttgttct cccgtgaact
     5941 ggacgatgaa caactgacgc aaatcgccag tgcgcagatg gctgaatggt tttcgttgct
     6001 gaaaagcgaa ccgccgctca ctgcggcggt gaatgatctg gaaaacagta ttgccac
//
LOCUS       DAJVRP010000165         6053 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVRP010000165 DAJVRP010000000
VERSION     DAJVRP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.165"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            431..1339
                     /locus_tag="OQN12_003808"
     CDS             431..1339
                     /locus_tag="OQN12_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCS1655970.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1385..3376)
                     /gene="tkt"
                     /locus_tag="OQN12_003809"
     CDS             complement(1385..3376)
                     /gene="tkt"
                     /locus_tag="OQN12_003809"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1655971.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3654..4412
                     /gene="loiP"
                     /locus_tag="OQN12_003810"
                     /gene_synonym="yggG"
     CDS             3654..4412
                     /gene="loiP"
                     /locus_tag="OQN12_003810"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1655972.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4618..5538)
                     /gene="speB"
                     /locus_tag="OQN12_003811"
     CDS             complement(4618..5538)
                     /gene="speB"
                     /locus_tag="OQN12_003811"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1655973.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5674..>6053)
                     /locus_tag="OQN12_003812"
     CDS             complement(5674..>6053)
                     /locus_tag="OQN12_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655974.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1490 a         1575 c         1563 g         1425 t
ORIGIN      
        1 gctgaatgta taactgagat tgatgattct ggaaaataat cactaagccg cataaattat
       61 ccttatttta tttttagggt accgtccggc ggtaaaaata aaatactcgt gcggtttaat
      121 gaaagcaatt gggtacgcga gaagaaaaac cccacaaacc aaaataataa aaccttaaat
      181 aacaacaata tgaaattgat atttttaaag tcaactttgt atttgttatg ttcatcacaa
      241 aatcaacatt ttgtttgtta ttgtatttgt gatgcatatc tctatttatt tattgaatgt
      301 tcgaaaacat acttttctaa tgcaatgttt tttcgctata cccctttata gattctgaat
      361 tctcacctgt cggctgtttt ctcctcatct catgtgacat attcccatac tttactcaag
      421 gaagattgtc atgaaagcac ctgtttcatt aacctcattt cgcccacaaa aatctcttgc
      481 tatcgccatt ggcgtgcttg ccgtggttgt gctgccattt ctctcgtatt acaccgttaa
      541 tgaaggtgaa cgcggcattt tgctgcgcta cggaaaaatc gtaaaagttg ccgaaccggg
      601 gctgggtttt aagatccctt ttatggaaag cgtggagaaa atttccacgc gtaatcaggc
      661 tgtggtctat cagggattac aggcctacag ccgcgaccag caaccggcac aaatgacagt
      721 atcagtcagc ttccacatta aaccttcaga agcaggcgcg gtttatacca cctacaacac
      781 tattgaagca ttaaaagatc gtctaattgt ccgtcagttg ccaactcagc tggagaacat
      841 tttcggccaa tataccgcca tcagcgccgt acaggatcgt acaaaattgg ttcaggatct
      901 gcaaaatgcc atgcgcaaag ccgttgttgg gccagtggta attgacgggg tccagattga
      961 aaatattgat ttttctgacg cttatgaaaa gtcgattgaa aaccgaatga aagcagaagt
     1021 cgccattgcc acgcgtaagc aaaatctgga aacagagaaa atccaggcgc aaatagcggt
     1081 aactcaggcc caggcagaag cagacagtaa actggcggca gccaaagtag aagcggaaac
     1141 tattcgcgta agaggcgcgg cagaggctga aactattcgt ttaaaaagtg cggctgaagc
     1201 agaagcaatc cgcctgcgcg gtgaagctct gcgggataat cccggacttg tagccctcac
     1261 cactgccgaa cgttgggacg gtaaactgcc cgataccatg atccccggca gcacagttcc
     1321 gtttatctct actaagtaaa aaaagggccg ctttcgcggt ccttctcaca aaacgtctta
     1381 ctgattacag cagcgctttt gctttcgcaa caacgttatc aacagtgaag ccgaactctt
     1441 caaacagcag ctctgccgga gcagattcac cgaaggtggt catgccgacg atagcgccgt
     1501 tcaggccaac atacttgtac cagtagtcag caatacccgc ttctaccgca acgcgtgcag
     1561 taaccgcttt cggcagtacg gattcacggt aagccgcatc ctgcttgtca aatgcgtcgg
     1621 tagacggcat ggacaccacg cgcgctttca cgccttcggc agtcagtttt tcgtaggcag
     1681 caaccgccag ttcaacttca gaaccggtgg cgatgaagat cagttccggc tgaccagcac
     1741 agtctttcag cacataacca ccgcgcgcga tgtttgccag ctgctcttca gtacgttcct
     1801 gctgcgccag gttctgacgg gagaggataa gcgcagtcgg gccgtcctga cgctcaacgc
     1861 cgtatttcca cgcgaccgcg gattcaacct ggtcacacgg acgccatgta gacatgttcg
     1921 gggtcacgcg cagagaagcg acctgctcta ccggctggtg agtcgggcca tcttcgccca
     1981 gaccgatgga gtcgtgggtg taaaccatca cctggcgctg tttcatcagc gcagccatac
     2041 gtacggcgtt acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac ggcaggaagc
     2101 caccgtgcag ggagataccg ttagcaatcg cggtcatacc gaactcgcga acaccgtagt
     2161 ggatgtagtt acctgcagca tcttcgttga ttgctttaga accagaccac agggtcaggt
     2221 tagacggtgc caggtcagca gagccgccga ggaattcagg caacagcggg ccgaacgctt
     2281 cgatagcatt ctgcgacgct ttacggctgg cgattttcgc cggattagcc tgcagtttag
     2341 cgataaactc tttcgctttg gcgtcgaagt cagacggcat ttcgcctttc atacggcggg
     2401 taaattcagc cgcttcctgc ggataagctt tcgcgtaagc cgcaaacttc tcattccatg
     2461 cagattcttt cgcctggcct gcttctttcg catcccactg agcatagatt tcagacggga
     2521 tttcgaacgg tgcatatttc cagcccagct gttcgcgggt cagggcaatt tcagcgtcgc
     2581 ccagcggcgc accgtgggag tcgtgagtac cggctttgtt cggggaaccg aaaccgatga
     2641 tggttttgca catcagcagg gacggtttgt cagtcactgc gcgcgcttct tcaactgcgc
     2701 gtttgatgga tgccgcgtca tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt
     2761 cgaaacgcat tgctgtgtcg tcggtgaacc agccttcaac gtgaccatcg atagaaatac
     2821 cgttgtcatc gtagaacgca atcagtttac ccagcttcag cgtacccgcc agagagcaaa
     2881 cttcgtggga gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt
     2941 cgacaatgtc gtggcccgga cggttaaact gcgccgccag cgttttttct gcaatcgcca
     3001 taccgactgc gttggcaata ccctgaccca gcggaccggt ggtggtttcc acaccagcgg
     3061 tataacctac ttccgggtgg cccggagttt tagagtgcag ctgacggaag tttttcagtt
     3121 cttccatcgg cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc atggagccgt
     3181 ggccgttgga cagcacgaag cggtcacggt cagcccagga cggattctgc gggttgtgtt
     3241 tcaggaaatc acgccacagg acttcggcaa tgtcagccat acccataggg gcacccgggt
     3301 gaccggattt ggctttctgt actgcgtcca tgctcagcgc acgaatagca ttggcaagct
     3361 ctttacgtga ggacattttg actccagatc ggatgatgaa gggcacgccc ttaacgactt
     3421 gacgacagcg cgttttgggc tacgccggaa aatttgccaa caatttaccg caagccgcgc
     3481 gtcatgtaca tggaacatcc ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt
     3541 gcgcaatctg ctcgcccgtc cgctgcgctt ttccttatac tgagactgag cgtcgattca
     3601 cctgcaaacg gcgcattttt agaataatcc tgaccttgtg cggaagagaa aacatgaaaa
     3661 ttcgcgcctt attggtagca atgagcgtgg caacggtact gaccggttgc cagaatatgg
     3721 actccaacgg actgctctca tcaggagcgg aagcttttca ggcttacagt ttgagtgatg
     3781 cgcaggtgaa aaccctgagc gatcaggcat gtcaggagat ggacagcaag gcgacgattg
     3841 cgccagccaa tagcgaatac gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca
     3901 atatcaacgg tcagccggtg aattacaaag tgtatatggc gaaggatgtg aacgcctttg
     3961 caatggctaa cggctgtatc cgcgtctata gcgggctgat ggatatgatg acagataacg
     4021 aagtcgaagc ggtgatcggt cacgagatgg ggcacgtggc gttaggccat gtgaagaaag
     4081 ggatgcaggt ggcgcttggt acaaatgccg tgcgagtagc tgcggcctct gcgggcggga
     4141 ttgtcggcag tttatctcaa tcacaacttg gtgatctggg cgagaaatta gtcaattcgc
     4201 aattctccca gcgtcaggaa gcagaagccg atgattattc ttacgatctt ctgcaccaac
     4261 gcggcatcag cccggcaggt cttgccacca gctttgaaaa actggcgaag ctggaagaag
     4321 gccgtcaaag ctcaatgttt gacgaccatc ctgcatccgc cgaacgtgcc cagcatattc
     4381 gcgatcgcat cagcgcggat gggattaagt aaagcctagt ggtgtcgaac gtaggtcaga
     4441 taaggcgttc acgccgcatc tgacattaat ggcacatttg ttaccttgtg cgcattgccg
     4501 gatgcgatgc tggcgcatct tatccggcct acgggtgccg aacgtaggtc ggataaggcg
     4561 ttcacgccgc atccgacatt aatggcacgt tttacccgtg cgcatcgcat ctggtgctta
     4621 ctcgcccttt ttcgccgcct gaatatacag catctccagc gccagcgtcg ccgcagccag
     4681 agcggtgatt tccgactgat cgtatgccgg agccacttcc actacgtcca tcccaacgat
     4741 gttgagatct ttcaggccgc gtaccagttt aatggcgcga tcggaggtca ggccgccaat
     4801 cactggcgta ccggttcccg gcgcgaaagc aggatccagg cagtcgatat caaaagtcag
     4861 gtaaaccggc atatcaccca caatctgttt cacctgggcg ataacgtcat ccacgctgcg
     4921 atcgttcacc tggcaggcgt ccagcacggt aaagccgttg tctttatcaa actcggtacg
     4981 aataccaatc tgcacggaat gattcgggtc gatcagacct tctttcggcg cggtatagaa
     5041 catagtgccg tggtcaaatt cacaaccgtt cgcataggta tcggtgtggg cgtcaaagtg
     5101 taccagcgcc attttgccga aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa
     5161 gtggtcacca ccgaaggaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc
     5221 ctgcagcttt tcgctcatct cacgggcatc gccaaaggca tataccagat cgccgcagtc
     5281 cacgacgttc agacgctcac gcatgtcgaa attccacggg aagcggttgt gttcccaggc
     5341 cagattcgtc gaaacctgac ggatcgctgc cggaccgtgg cgaccacccg cacgaccaga
     5401 agtggccata tcgaacggca cgccagtaat cacccagtct gcatcgctgt catacggctg
     5461 gaagttcatc ggcaggcgta aaaaaccaaa ggcattggaa accagtgagt tatcgtattg
     5521 atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc
     5581 gcgtcgttag gcccgacgag gaagggttgg atttgcgaaa cacaagtttg tgctaattat
     5641 cgccttaaat gggcgaggca tcaagcgagg ttgctatttc cgacacggtg gcggcgcata
     5701 gccattgtta tctgccggaa aggcttgtcc agagcacggc ggcagcgcgt tttgcgccgg
     5761 gccttgtaat tcaaaggcgg caaagccgat taccccggta ttacgcacat cgccttgcgc
     5821 cgaattggct gcgtaggaat cagcaacgtt ggcgaactga aaagccgctt cagtatgttt
     5881 atctttgcgg aatcctttaa tcactaacga atctccggca ttcacaatat agccgttgtt
     5941 attcagcgaa ccttgcttgc cgttcagcac atccaggcca tcagccgtag caacgatctc
     6001 gtagttggtg ttacgactgt aattacggac atataattga taattcattc cga
//
LOCUS       DAJVRP010000166         6028 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVRP010000166 DAJVRP010000000
VERSION     DAJVRP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.166"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="OQN12_003813"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="OQN12_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS1655975.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="OQN12_003814"
     CDS             1207..1869
                     /locus_tag="OQN12_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1655976.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="OQN12_003815"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="OQN12_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1655977.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="OQN12_003816"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="OQN12_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="OQN12_003817"
     CDS             2795..3100
                     /locus_tag="OQN12_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1655978.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="OQN12_003818"
     CDS             3103..3507
                     /locus_tag="OQN12_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1655979.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="OQN12_003819"
     CDS             3497..3796
                     /locus_tag="OQN12_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1655980.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="OQN12_003820"
     CDS             3982..4278
                     /locus_tag="OQN12_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1655981.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="OQN12_003821"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="OQN12_003821"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCS1655982.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="OQN12_003822"
     CDS             5627..5995
                     /locus_tag="OQN12_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1655983.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1524 a         1470 c         1519 g         1515 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaatt
//
LOCUS       DAJVRP010000167         5983 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVRP010000167 DAJVRP010000000
VERSION     DAJVRP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.167"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..262)
                     /locus_tag="OQN12_003823"
     CDS             complement(<1..262)
                     /locus_tag="OQN12_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCS1655984.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            483..911
                     /gene="uspG"
                     /locus_tag="OQN12_003824"
     CDS             483..911
                     /gene="uspG"
                     /locus_tag="OQN12_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1655985.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="OQN12_003825"
     CDS             complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="OQN12_003825"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1655986.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="OQN12_003826"
     CDS             complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="OQN12_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1655987.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3777..4523
                     /gene="dsbG"
                     /locus_tag="OQN12_003827"
     CDS             3777..4523
                     /gene="dsbG"
                     /locus_tag="OQN12_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1655988.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4733..5635
                     /locus_tag="OQN12_003828"
     CDS             4733..5635
                     /locus_tag="OQN12_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1655989.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5782..5983
                     /locus_tag="OQN12_003829"
                     /pseudo
     CDS             5782..5983
                     /locus_tag="OQN12_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1605 a         1441 c         1348 g         1589 t
ORIGIN      
        1 tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt
       61 caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt
      121 tgcctcgata aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa
      181 tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc
      241 cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt
      301 aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt
      361 gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata
      421 tcagggataa atattataat catggagaat gatttgttca tgattaacag ggagaaaggg
      481 ttatgtataa ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag
      541 ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac
      601 tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag
      661 agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg
      721 atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt
      781 tggcagaaga actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga
      841 cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg
      901 tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt
      961 tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat
     1021 cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct
     1081 ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc
     1141 gctgcgaaca cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg
     1201 cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca
     1261 aaaataccgg ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga
     1321 ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg
     1381 acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca
     1441 aattccagca gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg
     1501 gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcggtcc gtcgcagtgc
     1561 gggcagtagg tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc
     1621 cattttgcac ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt
     1681 tcaatctggt gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg
     1741 tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc
     1801 ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca
     1861 ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc
     1921 gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc
     1981 tcttctgccg cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc
     2041 atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg
     2101 cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg
     2161 ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc
     2221 gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac
     2281 tgcgcttctt tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac
     2341 tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc
     2401 aggaaagacg gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac
     2461 agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc
     2521 gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc
     2581 atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct
     2641 gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac
     2701 ccgaagaatt aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga
     2761 atccccggga gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat
     2821 ggcgtgaaag acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt
     2881 tgcttcacct tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg
     2941 agctgctttg attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac
     3001 ttcgattgcc tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc
     3061 ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat
     3121 cgcatatttg attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg
     3181 agtatcggta gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc
     3241 agcaacgtca cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa
     3301 gacgctccag cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa
     3361 tgcctggttt ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt
     3421 gttttcgatg agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat
     3481 caaatcgata agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc
     3541 agcggcgata aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct
     3601 ttggtatctt cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac
     3661 atggattaca acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga
     3721 cagtgatagg ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt
     3781 taaaaaagat acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt
     3841 ccccagtaaa agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg
     3901 gaggaatgaa aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc
     3961 ccgatggtaa gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta
     4021 acacacttat cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg
     4081 aacaatccca ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct
     4141 ccgatccgtt ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt
     4201 ctggcaaagt gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga
     4261 cagcagcggc aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct
     4321 ctggttgcaa gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt
     4381 taagtgacaa tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt
     4441 acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta
     4501 atatcattat gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt
     4561 ttcaaccatc ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta
     4621 gaaaaactta atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa
     4681 acattgacta agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa
     4741 tctctacgac ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt gtatttacca
     4801 gcatttaagc attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca
     4861 gtcgttacaa cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg
     4921 tatcgcgccg acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg
     4981 ccttgaacaa acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta
     5041 cggccctcag ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga
     5101 tgcctctgtc gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact
     5161 gttggttcac cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt
     5221 catttgcatg ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg
     5281 aattactgac aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatagc
     5341 caaatcagca ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa
     5401 aatttcattt cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt
     5461 actgggaata gtcccctatg aactttataa ctaccaccgt gactttctta atttaaaaga
     5521 gataaaacta gaacatccgt taccttcaat aaagctttac atttcctata ataaatcttc
     5581 tttaaataat ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat
     5641 aactatagac agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa
     5701 cactctgctt tacagtgttt actttataat taacagtctt atctcactgt tcaccctgta
     5761 acgttcagag agcgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg
     5821 gaagctgcac gagagcgtat tacctagaca ctcaacaccc taccccgcgt ttgtgtctca
     5881 ttttctggcg gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa
     5941 atgggcaaaa aaatctgcgt tttgtttatc aactgggagg cgc
//
LOCUS       DAJVRP010000168         5818 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVRP010000168 DAJVRP010000000
VERSION     DAJVRP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.169"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..369
                     /locus_tag="OQN12_003830"
     CDS             <1..369
                     /locus_tag="OQN12_003830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1655990.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OQN12_003831"
     CDS             complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OQN12_003831"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS1655991.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OQN12_003832"
     CDS             complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OQN12_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS1655992.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OQN12_003833"
     CDS             complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OQN12_003833"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCS1655993.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OQN12_003834"
     CDS             complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OQN12_003834"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS1655994.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OQN12_003835"
     CDS             complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OQN12_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCS1655995.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5094..>5818)
                     /locus_tag="OQN12_003836"
     CDS             complement(5094..>5818)
                     /locus_tag="OQN12_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCS1655996.1"
                     /translation="VKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSK
                     KIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEI
                     KALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVAL
                     AVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPA
                     SEQPPAQPRLRITEQDPNWETF"
BASE COUNT         1312 a         1693 c         1385 g         1428 t
ORIGIN      
        1 actacgctaa tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt
       61 aaagataatt acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac
      121 cccaatccaa gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag
      181 agtttagggc cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa
      241 gtatggcatc actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt
      301 aatgcattga taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attatcaaac
      361 tacaaataac catcctccac gtactccctg aacgataccc gccaccctgc tatcggcttg
      421 gcatcatcgc gcaggccgaa gttcaggcaa tacaaatcaa aatccaagac tatccaacaa
      481 atcgtccacc tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc
      541 attaagcaaa ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa
      601 cagcaccatc agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac
      661 ctgcccggtg agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt
      721 aaagctggta tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg
      781 ggcgtcggca aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt
      841 tgctgatttc tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg
      901 ctccgcagcc tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat
      961 ggcttccgcc gcttcggcaa tggcctgatc cagccccagt tcccgcaaac tgtcgcgcag
     1021 catatgcgtc aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg
     1081 tttgattgat ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt
     1141 tgagtttttc ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg
     1201 cttgcgccgc agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca
     1261 atgccgacat cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg
     1321 gcatgttcca gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt
     1381 cgacaccatc ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca
     1441 ctatgcgtcg catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca
     1501 tttcacactc ctgatttaaa tacgtatcgc ctgtccggca ctaattgttg ccaacatttg
     1561 ctggcttacc tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg
     1621 cggcatgccg aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg
     1681 acgcatcgcc aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac
     1741 cgcattacgc cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg
     1801 acggttaacc gccgggccgt cgtgaatttt gatttgataa tttgcgccac tacgcgccag
     1861 ttccatatgc cgatcgcccg gcgcaatata ggcatgcccc ggcaggacac gttctccgtc
     1921 ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt
     1981 gaaaccgggc ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg
     2041 cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc
     2101 agaactcaac aacggccctg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc
     2161 aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat
     2221 tccttcgcga atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc
     2281 gcgcagcgtg acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg
     2341 acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc
     2401 cagcgtcagc acatcgggat tgaatttctt aatcaagtca cgcgcaacca gcggatcagg
     2461 cgcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg
     2521 catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt
     2581 agcgcataca ccgtctgacc acgcagcgtg aagcggcgct cgaggtggct aaagttttca
     2641 gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc
     2701 tcctgctggg tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac
     2761 ggccccggca cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc
     2821 gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac
     2881 cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgatagat accgcttctg
     2941 gctttttcca gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcacg
     3001 gtgcccaatg tgtcagccag agtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc
     3061 gccgcgctcc atacgcgatc ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg
     3121 aaatgatgag cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc
     3181 tgccactcac cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc
     3241 agtcccagcg aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca
     3301 gccagaacga tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg
     3361 gaaagcgcca ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat
     3421 gaagtcatag cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc
     3481 gactcatgtc gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga
     3541 tggtcggcct gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggag
     3601 ttctgctgag tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc
     3661 cgttgttctt ccgacgccga agcaatttct cccataatgt cgttcacgcg ggtcaccgaa
     3721 ctgacaatat cggtcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca
     3781 cgattgactg actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg
     3841 gcaagattgc gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga
     3901 gccgcttcca ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata
     3961 acgctgataa tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc
     4021 atggtactga cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc
     4081 gacgcctgtc gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg
     4141 gcggccgttt gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg
     4201 cctgcgacaa tctccgcgat cccaatgtgc atctcatggc ttcccttacg cacatcactc
     4261 accgtcccac gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca
     4321 ttacgaccat ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg
     4381 ctaccgataa tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg
     4441 atgtagatcg ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta
     4501 cgctggcttt gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca
     4561 aagttaacgt caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt
     4621 tgattacttt caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt
     4681 ttctccgtct ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa
     4741 cttttaaaca gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta
     4801 atgtcatccg ccggatagct aagcgctgtc agagtgcctg ctttgttcag cgcggtactg
     4861 gcctgtaaca ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg
     4921 ccgtgattca gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca
     4981 atctgcaaga tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga
     5041 ttaaacatca acgctcccca tcaggcggca atgaccgcgt tagtaaaaac tcgtcaaaat
     5101 gtttcccagt ttggatcttg ttcagtaatt cgcagtcgtg gctgtgccgg tggttgctca
     5161 ctggcaggac gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac
     5221 gcggaaaccg cttgcgttaa acgactcgcc tgttcttcca gcgcagctgc ggcggcagct
     5281 gattcctgca ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat
     5341 gcgacttgat cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg
     5401 tcagtcacgc gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc
     5461 agcaccgaac cggtatcaat gcgcgagacg gagtcttcaa tgagggcttt gatctctttt
     5521 gccgcctggg cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca
     5581 cggccctgtt cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc
     5641 tggaaggcaa taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg
     5701 atctcatgca tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg
     5761 gaggcacttt gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactg
//
LOCUS       DAJVRP010000169         5784 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVRP010000169 DAJVRP010000000
VERSION     DAJVRP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.170"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..941)
                     /gene="hprS"
                     /locus_tag="OQN12_003837"
     CDS             complement(<1..941)
                     /gene="hprS"
                     /locus_tag="OQN12_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1655997.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(941..1612)
                     /gene="hprR"
                     /locus_tag="OQN12_003838"
     CDS             complement(941..1612)
                     /gene="hprR"
                     /locus_tag="OQN12_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1655998.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1745..2158
                     /gene="hiuH"
                     /locus_tag="OQN12_003839"
     CDS             1745..2158
                     /gene="hiuH"
                     /locus_tag="OQN12_003839"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1655999.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2267..3271
                     /gene="msrP"
                     /locus_tag="OQN12_003840"
                     /pseudo
     CDS             2267..3271
                     /gene="msrP"
                     /locus_tag="OQN12_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3272..3907
                     /gene="msrQ"
                     /locus_tag="OQN12_003841"
     CDS             3272..3907
                     /gene="msrQ"
                     /locus_tag="OQN12_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS1656000.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4165..4815
                     /gene="zinT"
                     /locus_tag="OQN12_003842"
     CDS             4165..4815
                     /gene="zinT"
                     /locus_tag="OQN12_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1656001.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1643 a         1304 c         1196 g         1641 t
ORIGIN      
        1 ttattgagag aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga
       61 aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt
      121 gttttttgat attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt
      181 aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc
      241 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      301 tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc
      361 gttacgccac tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat
      421 actgagcaca gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca
      481 agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      541 tcatcaatgt taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg
      601 cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
      661 gtccggttaa caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca
      721 tccatcatcc ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac
      781 tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
      841 gccaggccat tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata
      901 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
      961 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1021 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1081 cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta
     1141 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac
     1201 tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat
     1261 gttgccttaa ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca
     1321 gataatcatt tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag
     1381 taaggcaaat aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat
     1441 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1501 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1561 gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa
     1621 tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct
     1681 gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat
     1741 aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc
     1801 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg
     1861 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca
     1921 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     1981 tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2041 tcttgaaagt ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca
     2101 ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg
     2161 cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa
     2221 cagtaaacag ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca
     2281 atttttaaaa gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt
     2341 gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga
     2401 tctgcttagc tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga
     2461 gttcagcaag cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc
     2521 cggttataac aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg
     2581 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     2641 cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     2701 ctgcgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact
     2761 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc
     2821 ggaacagatg cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     2881 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta
     2941 tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata
     3001 tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac
     3061 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3121 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga
     3181 tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3241 tcgtggcctg gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg
     3301 gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3361 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3421 tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca
     3481 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3541 gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa
     3601 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     3661 tttagcgttc acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt
     3721 gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     3781 gattatttca ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg
     3841 gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc
     3901 tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc
     3961 cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca
     4021 ccagaataat ttattttatt tcaataagat agttcaatac acctgagaat accatgctct
     4081 catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt
     4141 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
     4201 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc
     4261 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
     4321 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
     4381 aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
     4441 attaaagatt attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac
     4501 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
     4561 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
     4621 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
     4681 aaatcatctc attttcacat ttttatgggt aatgattccc agcaattttt attgaatgaa
     4741 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
     4801 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
     4861 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
     4921 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata
     4981 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
     5041 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
     5101 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     5161 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     5221 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     5281 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     5341 cctgtcagct ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga
     5401 cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag
     5461 tcgggagata agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt
     5521 tgcccctact ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa
     5581 tttttcttgg aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt
     5641 aaagaagaat attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg
     5701 tgatttgccc cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt
     5761 gttgaataat tccactgcag ttat
//
LOCUS       DAJVRP010000170         5682 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVRP010000170 DAJVRP010000000
VERSION     DAJVRP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.171"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="OQN12_003843"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="OQN12_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1656002.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="OQN12_003844"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="OQN12_003844"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1656003.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="OQN12_003845"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="OQN12_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1656004.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="OQN12_003846"
     CDS             complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="OQN12_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCS1656005.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1456 a         1487 c         1349 g         1390 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcata gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cagaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4981 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     5041 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     5101 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     5161 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     5221 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     5281 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     5341 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5401 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5461 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5521 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5581 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5641 actaatagtg cgcccagcag ataaaatgtg aatttaatca gt
//
LOCUS       DAJVRP010000171         5539 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVRP010000171 DAJVRP010000000
VERSION     DAJVRP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.172"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..642
                     /locus_tag="OQN12_003847"
     CDS             <1..642
                     /locus_tag="OQN12_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCS1656006.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="OQN12_003848"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="OQN12_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1656007.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="OQN12_003849"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="OQN12_003849"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1656008.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1371 a         1354 c         1539 g         1275 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagata
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagctat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttt
//
LOCUS       DAJVRP010000172         5404 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVRP010000172 DAJVRP010000000
VERSION     DAJVRP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.173"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..501
                     /locus_tag="OQN12_003850"
     CDS             <1..501
                     /locus_tag="OQN12_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCS1656009.1"
                     /translation="YNEIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRL
                     HLIANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAF
                     EKLAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMS
                     HAESAH"
     gene            523..2514
                     /gene="cyoB"
                     /locus_tag="OQN12_003851"
                     /pseudo
     CDS             523..2514
                     /gene="cyoB"
                     /locus_tag="OQN12_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2504..3118
                     /locus_tag="OQN12_003852"
     CDS             2504..3118
                     /locus_tag="OQN12_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS1656010.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3118..3447
                     /locus_tag="OQN12_003853"
     CDS             3118..3447
                     /locus_tag="OQN12_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS1656011.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3459..4349
                     /gene="cyoE"
                     /locus_tag="OQN12_003854"
     CDS             3459..4349
                     /gene="cyoE"
                     /locus_tag="OQN12_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS1656012.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4534..4845
                     /locus_tag="OQN12_003855"
     CDS             4534..4845
                     /locus_tag="OQN12_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1656013.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4980..5378
                     /locus_tag="OQN12_003856"
     CDS             4980..5378
                     /locus_tag="OQN12_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1656014.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1183 a         1343 c         1424 g         1454 t
ORIGIN      
        1 tacaatgaaa tcgctttccc ggcgaacact ccggtgtact tcaaagtgac ctccaactcc
       61 gtgatgaact ccttcttcat tccgcgtctg ggtagccaga tttatgccat ggccggtatg
      121 cagactcgcc tgcatctgat cgccaacgaa cccggtactt atgacggtat ctccgccagc
      181 tacagcgggc cgggcttctc aggcatgaag ttcaaagcta ttgcaacacc ggatcgcgcc
      241 gaattcgacc aatgggtcgc aaaagcgaaa cagtcgccga ataccatgtc tgacatggct
      301 gcgttcgaaa aactggccgc gcctagcgaa tacaaccagg tggaatattt ctccaacgtg
      361 aaaccagact tgtttgccga tgtgattaac aagtttatgg ctcacggtaa gagcatggac
      421 atgacccagc cagaaggtga gcacagtgca cacgaaggta tggaaggcat ggacatgagc
      481 cacgcggaat ccgcccatta aaggggttga ggaagaataa agatgttcgg aaaattatca
      541 cttgatgcag tcccgttcca tgaaactatc gtcatggtta cgatcgctgg cattattttg
      601 ggaggtctgg cgctcgttgg cctgatcact tacttcggta agtggaccta cctgtggaaa
      661 gagtggctga cctccgtcga ccataaacgc ctcggtatca tgtatatcat cgtggcgatt
      721 gtgatgttgc tgcgtggttt tgctgacgcc attatgatgc gtagccagca ggctcttgcc
      781 tcggcgggtg aagcgggctt cctgccacct caccactacg atcagatctt caccgcgcac
      841 ggcgtgatta tgatcttctt cgtggcgatg cctttcgtta tcggtctgat gaacctggtg
      901 gttccgctgc agatcggcgc gcgtgacgtt gcgttcccgt tcctcaacaa cttaagcttc
      961 tggtttactg ttgttggcgt gattctggtt aacgtttctc tcggcgtggg cgaatttgcg
     1021 cagaccggct ggctggccta tccaccgcta tcgggaatag agtacagtcc gggagtcggt
     1081 gttgattact ggatatggag tctccagcta tccggtatag gtacgacgct taccggtatc
     1141 aacttcttcg ttaccattct gaagatgcgc gcaccgggca tgaccatgtt caagatacca
     1201 gtatttacct aggcatcact gtgcgcgaac gtactgatta ttgcttcctt cccaattctg
     1261 acggttaccg tcgcgttgtt gaccctggat cgctatctgg gcacccattt ctttaccaac
     1321 gatatgggtg gcaacatgat gatgtacatc aacctgattt gggcctgggg ccacccggaa
     1381 gtttacatcc tgatcctgcc tgttttcggt gtgttctccg aaattgcggc aaccttctcg
     1441 cgtaaacgtc tgtttggtta tacctcgctg gtatgggcaa ccgtctgcat caccgtgctg
     1501 tcgttcatcg tttggctgca ccacttcttt acgatgggtg cgggcgcgaa cgtaaacgcc
     1561 ttctttggta tcaccacaat gattatcgcc atcccgaccg gggtgaagat cttcaactgg
     1621 ctgttcacca tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg gactatcggt
     1681 tttatcgtca ccttctcggt gggcggtatg actggcgtgc tgctggcagt accgggcgcg
     1741 gacttcgttc tgcataacag cctgttcctg attgcgcact tccataacgt gatcatcggc
     1801 ggcgtggtct tcggctgctt cgcagggatg acctactggt ggcctaaagc gttcggtttc
     1861 aaactgaacg aaacctgggg taaacgcgcg ttctggttct ggatcatcgg cttcttcgtt
     1921 gcctttatgc cgctgtatgc gctgggcttc atgggcatga cccgtcgttt gagccagcag
     1981 attgacccgc agttccacac catgctgatg attgcagcca gcggtgcagt actgattgcg
     2041 ctgggtattc tctgcctcgt tattcagatg tacgtttcta ttcgcgaccg cgaccagaac
     2101 cgtgacctga ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac ctcttccccg
     2161 cctccgttct ataactttgc cgtagtgccg cacgttcacg aacgtgatgc attctgggaa
     2221 atgaaagaga aaggcgaagc gtataaaaag cctgaccact atgaagaaat tcatatgccg
     2281 aaaaacagcg gtgcaggtat cgtcattgca gctttctcca ccatcttcgg tttcgccatg
     2341 atctggcata tctggtggct ggcgattgtt ggcttcgcag gcatgatcat cacctggatc
     2401 gtgaaaagct tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg
     2461 gaaaaccagc atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt
     2521 gacgcacgcg actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat
     2581 cttcggattt tggatctacc tgatgagcga ctgcattctg ttctcaatct tgtttgctac
     2641 ctatgccgtt ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact
     2701 gccgttcgtt ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc
     2761 ggctatcgcc atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg
     2821 gttgtttggt gccggattta tcgggatgga aatctatgaa ttccatcacc tgattgttaa
     2881 cggcatgggg ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca
     2941 cggtctgcac gtcacttctg gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg
     3001 tcgcggcctg accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt
     3061 cctggatgtg gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg
     3121 agtcattcta ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc
     3181 tttatcctgt cgatcattct gacggtgatt ccgttctgga tggtgatgac aggggctgcc
     3241 tctccggccg taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat
     3301 ctggtgtgct tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt
     3361 gtcttcaccg tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac
     3421 ctcaactaca acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc
     3481 aagtaacgaa accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc
     3541 tggcctcaaa gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac
     3601 tggttgtgac gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga
     3661 tggaaaggac gaagaatcgg gtgctggtta aaggcctgat ctctcctgct gtctcgctgg
     3721 tgtacgccac cttgctgggt attgctggct ttatgctgct gtggtttggc gcgaatccgc
     3781 tggcctgctg gctgggggtg atgggctttg tggtttatgt cggcgtctat agcctgtaca
     3841 tgaaacgcca ctcagtctac ggcacattga ttggttcgct ctccggcgct gcgccgtcgg
     3901 tgattggcta ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc ctgctggcta
     3961 tcttcagcct gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt
     4021 accaggcggc aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc
     4081 acatcacgct gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt
     4141 acgctgggta taaatatctg gtggtcgccg cggcggttag cgtctggtgg ttaggtatgg
     4201 ctctgcgcgg ttataaagtt gctgatgaca gaatctgggc gcgcaagctg ttcggcttct
     4261 ctatcatcgc catcactgcc ctctcggtga tgatgtccgt tgattttatg gtaccggact
     4321 cgcatacgct gctggctgct gtgtggtaac aaaacctctc tattaaaaag gtgctacggc
     4381 acctttttta ttgtctgaga tcagagatac ggctaataac cgtatctctt gacagagtga
     4441 gcagatgcac ataaattaat caaacggttg atagccgtat tatgtatgag atcgactccc
     4501 tacgatttca ttcagttaat caaagaagtc ataatgcctg gaaaaacagc cgctctatac
     4561 gatgtcgata aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc cgacgccaaa
     4621 aacaaacttc gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt tattttgagc
     4681 gctgctatgg aacgcgccga aagtgtgctt gataaccaac gtcgccgtga gctttcgaat
     4741 caaagctggg aactgatgaa ccaactcatc gctgaacctg ctcaaccgac gctcgccctc
     4801 aaggcgttaa tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga ttactctgcc
     4861 catagtcctc tcctgtaatt atcagtctga tattacttat cctgggcaaa aacagtttga
     4921 ttgcggtaac cctgttatcg ataaatttgt acgcgctacg cgcatcgcta aagaaaagtg
     4981 tgcgtaatag cgactgtgcg gctaaagcac ttattgacag acaaagtggt gaactgattg
     5041 gcatctgtac ttttacggca tattcgctgg aaagacaacg cgtttctggc gtccttcagg
     5101 gttcacaacc ttcagaaatt ggtgttgtca gattagtcat gttgggggga gcgcggaagt
     5161 atcaaaagcg gggctttggt caggacctac tatgtgattt ttttgaacat gtaaaaaaaa
     5221 ttcacccggc attaccaatt aaaggggttt atcttgatgc tgaccctgcc gccattaact
     5281 tttatgctcg tctcggcttt gttcagcttt cagcgacacc aaatgctttt ggtgctgtac
     5341 ctatgttttt ggcgattcag catattctcg cggcttagat tattatcgcc acccgccagg
     5401 gcaa
//
LOCUS       DAJVRP010000173         5388 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVRP010000173 DAJVRP010000000
VERSION     DAJVRP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.174"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..945
                     /gene="tauA"
                     /locus_tag="OQN12_003857"
     CDS             <1..945
                     /gene="tauA"
                     /locus_tag="OQN12_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1656015.1"
                     /translation="NTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESG
                     ASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEAL
                     VVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQ
                     RGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAF
                     AKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAEL
                     TGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            958..1725
                     /gene="tauB"
                     /locus_tag="OQN12_003858"
     CDS             958..1725
                     /gene="tauB"
                     /locus_tag="OQN12_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS1656016.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1722..2549
                     /gene="tauC"
                     /locus_tag="OQN12_003859"
     CDS             1722..2549
                     /gene="tauC"
                     /locus_tag="OQN12_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1656017.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2546..3397
                     /gene="tauD"
                     /locus_tag="OQN12_003860"
     CDS             2546..3397
                     /gene="tauD"
                     /locus_tag="OQN12_003860"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS1656018.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3503..4477)
                     /gene="hemB"
                     /locus_tag="OQN12_003861"
     CDS             complement(3503..4477)
                     /gene="hemB"
                     /locus_tag="OQN12_003861"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1656019.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5001..5369
                     /locus_tag="OQN12_003862"
                     /pseudo
     CDS             5001..5369
                     /locus_tag="OQN12_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1252 a         1358 c         1490 g         1288 t
ORIGIN      
        1 aacacacttc ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc
       61 gtggcgtatc aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct
      121 aaagagagcg gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg
      181 gcgctggctt ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca
      241 gccagccaac aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa
      301 gcgctggtgg taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc
      361 gtaccgttta tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt
      421 aaaccagggc tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag
      481 cggggtgata ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac
      541 ggcaaggtgc tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc
      601 tgggtggtgc gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa
      661 agcgccatcg atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg
      721 gaaaacatca gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg
      781 gtgaagggaa atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg
      841 aacaaagcaa tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta
      901 gcgaatgatt acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg
      961 ctgcaaatct ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac
     1021 ctgacgctgg aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc
     1081 accctgctga atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg
     1141 ggtaagcgta ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg
     1201 ctaccgtggc gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag
     1261 aaaatgcagc gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca
     1321 gaaaaacgct atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc
     1381 gcgctggcgg cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc
     1441 ttcacccgcg accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag
     1501 gtgctgttga ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg
     1561 ctttcatccg gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt
     1621 gttgcgggag agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa
     1681 tatgttttaa gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg
     1741 aaaaactgca ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa
     1801 gcattggcac gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga
     1861 ttagcccgct gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg
     1921 gaccgcaggg atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca
     1981 ttgtgctggc gctactcgcg gcggtggtga tcggtattcc ggtcgggatc gccatggggc
     2041 taagcccgac ggtgcgcggc attctggatc cggtaatcga actttatcgt ccggtgccac
     2101 cgctggctta tttgccgctg atggtgatct ggtttggtat tggtgaaacc tcgaagatct
     2161 tactgatcta tttagcgatt tttgcgccgg tggcgatgtc ggcgctggca ggggtgaaaa
     2221 gcgcgcagca ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt gcgcaggtgc
     2281 tgtggtttgt cattttgccc ggcgcgctgc cggaaatcct caccggatta cgcattggtc
     2341 tgggtgtggg ctggtctacg ctggtagcgg cggagctgat tgccgcgaca cgcggtttag
     2401 gatttatggt tcagtcagcg ggtgaatttc tcgcgactga cgtggtactg gcggggatcg
     2461 cggtgattgc gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgtc
     2521 tgacgccctg gcatggagaa gtacaatgag tgaacgtctg agcattaccc cgctggggcc
     2581 gtatatcggc gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt
     2641 tgaacagctt taccatgcgg tgctgcgcca tcaggtggta tttctgcgcg atcaagctat
     2701 tacgccgcag cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt
     2761 ttaccctcat gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc
     2821 gccggataac gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc
     2881 gattctggca gctaaagagt taccttcgac cggcggtgat acgctctgga ccagcggtat
     2941 tgcggcctat gaggcgctct ctgttccctt ccgccagctg ctgagctggc tgcgtgcgga
     3001 gcatgatttc cgtaaatcat tcccggaata caaataccgc aaaaccgagg aggaacatca
     3061 acgctggcgc gaggcggtcg cgaaaaatcc tccgttgcta catccggtgg tgcgaacgca
     3121 tccggtgagc ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgtcga
     3181 tgtcagcgag aaagagagcg aagccttgtt aggcttttta ttcgcccata tcactaaacc
     3241 ggagtttcag gtgcgttggc gctggcaacc gaatgatatt gcgatttggg ataaccgcgt
     3301 gactcagcac tatgccaatg ccgattacct gccacagcgg cggattatgc atcgggcgac
     3361 gatccttggg gataaaccgt tttatcgggc ggggtgatac gagtgcggac ggttccctcg
     3421 cccccttggg gagagggtta gggtgagggg gcgttcactg tactttcaac aggttaactc
     3481 cccctttctg agaggaaaaa aattaacgca gaatcttctt ctcagccaaa tccagcgcaa
     3541 agtagctgaa aatcagatcc gcacccgcac gcttaattga acctaagctt tcgagcacga
     3601 ctttctcttc atctatagca cccgccagcg cggcgaactt aatcatcgcg tactcaccgc
     3661 tcacctgata cgcgccaatc ggcaattcag tacgttcacg cagctcacgc acgatgtcga
     3721 ggtacgctcc agcaggttta accatcaggc agtctgcgcc ctgggcttca tccagcagtg
     3781 actcacgaat cgcctcgcga cggttcattg ggttcatctg atagcttttg cggtcgcctt
     3841 ttaatgcgct tcctgcagct tcacggaacg ggccataaaa ggaagaggcg aacttggtcg
     3901 aatacgacat aatcgccgta tctttaaagc ctgcagcgtc cagcgcctga cgaatcgcct
     3961 gtacctggcc gtccatcgcg gcggaagggg cgatgaagtc tgcgcctgca gcagctgcaa
     4021 ccacggcttg cttgcctaaa ttttccagag tcgcgtcgtt gtcgacgcca tgctcgcaca
     4081 gcacaccgca gtgaccgtga gaagtgtatt cacagaaaca agtgtctgac atgacgatca
     4141 tttctggcac agtctgcttg cagatgcgcg acatacgcgc caccagtcca tcttcccgcc
     4201 aggcgtcgct gccggtttca tcggtatggt gagagatgcc aaaagtcatt acggaacgga
     4261 taccggcgtt ggcgatgcgt tcaatttcgc gtgccagatg tttctctgga atgcgcatca
     4321 cgcctggcat ggcttcaacg gctttgtagt cgtcaatttc ttcttcaaca aagatcggca
     4381 acaccaggtc gttaaggcta agtgttgtct cttcaaacat agcgcgcagc gcaggagatt
     4441 tgcgcaggcg acgagggcgt tggattaagt ctgtcatggt ctgcctgatg tttgtggaat
     4501 taaggtgcac agtatacctg aagcggggca ggagtgtttt acgaaagttg tcgcgatgtt
     4561 gacaagaaga gaatgaaaga gagaccagga catctttgta atagaaatag caataatttc
     4621 atattcactg aaaatatttt taatctttat ttatagctct tggttattat tttttggcgt
     4681 ttggttgtgc tgctataaat tgaataattc aaactttgtt gcatattgat aagatattta
     4741 tctggtttgt gtttttgata tttataacgc gtacgcatca aaaataacat tatctgcaac
     4801 atactgaaat atcgtccttt tatcaatttg atgcgtgttt ttctccataa attatatatg
     4861 tccacattcg gacttagggg aaagaataat tgaaccattc gtctgtaacg cagcataatc
     4921 gttagcgcga aacataatat gttttctatg cattgataat tgatggatca acttattacg
     4981 tccctgagga gggatgacaa atgcactcct ggaaaaagaa acttgtagta tcacaattag
     5041 cattggcttg cactctggct atcacctctc aggctaatgc agcgaccaac gatatttctg
     5101 gtcaaactta caatactttc catcactaca acgacgccac ctatgctgat gacgtttact
     5161 atgatggtta tgtaggctgg aacaactatg ccgctgatag ctattacaac ggcgatatct
     5221 acccggtcat taataacgct accgttaacg gcgtgatttc tacctactat ctggacgacg
     5281 gtatttctac caataccaac gccaatagtc tgacaatcaa aaacagcact attcacggta
     5341 tgatttattc cgagtgcatg acaactgatt agactggccc cctgaatc
//
LOCUS       DAJVRP010000174         5387 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVRP010000174 DAJVRP010000000
VERSION     DAJVRP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.175"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..219)
                     /locus_tag="OQN12_003863"
     CDS             complement(<1..219)
                     /locus_tag="OQN12_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCS1656020.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="OQN12_003864"
     CDS             complement(216..1124)
                     /locus_tag="OQN12_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1656021.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /locus_tag="OQN12_003865"
                     /pseudo
     CDS             1320..2087
                     /locus_tag="OQN12_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="OQN12_003866"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="OQN12_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..>5387)
                     /gene="mutS"
                     /locus_tag="OQN12_003867"
     CDS             complement(2896..>5387)
                     /gene="mutS"
                     /locus_tag="OQN12_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1656022.1"
                     /translation="PEILLFYRMGDFYELFYDDAKRASQLLDISLTKRGASAGEPIPM
                     AGIPYHAVENYLAKLVNQGESVAICEQIGDPATSKGPVERKVVRIVTPGTISDEALLQ
                     ERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEPADRETMAAELQRSNPAELLYAEDF
                     AEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFGTRDLVGFGVENAPRGLCAAGCLLQ
                     YAKDTQRTTLPHIRSITMEREQDSIIMDAATRRNLEITQNLAGGAENTLASVLDCTVT
                     PMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDFTAELQPVLRQVGDLERILARLALR
                     TARPRDLARMRHAFQQLPELRAQLETVDSAPVQALREKMGEFAELRDLLERAIIDTPP
                     VLVRDGGVIASGYNEELDEWRALADGATDYLERLEVRERERTGLDTLKVGFNAVHGYY
                     IQISRGQSHLAPINYMRRQTLKNAERYIIPELKEYEDKVLTSKGKALALEKQLYEELF
                     DLLLPHLEALQQSASALAELDVLVNLAERAYTLNYTCPTFIDKPGIRITEGRHPVVEQ
                     VLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYMRQTALIALMAYIGSYVPAQKVEIG
                     PIDRIFTRVGAADDLASGRSTFMVEMTETANILHNATEYSLVLMDEIGRGTSTYDGLS
                     LAWACAENLANKIKALTLFATHYFELTQLPEKMEGVANVHLDALEHGDTIAFMHSVQD
                     GAASKSYGLAVAALAGVPKEVIKRARQKLRELESISPNAAATQVDGTQMSLLSVPEET
                     SPAVEALENLDPDSLTPRQALEWIYRLKSLV"
BASE COUNT         1268 a         1424 c         1385 g         1310 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggat
//
LOCUS       DAJVRP010000175         5364 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVRP010000175 DAJVRP010000000
VERSION     DAJVRP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.176"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..262)
                     /locus_tag="OQN12_003868"
     CDS             complement(<1..262)
                     /locus_tag="OQN12_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1656023.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPK"
     gene            complement(411..1124)
                     /gene="msyB"
                     /locus_tag="OQN12_003869"
     CDS             complement(411..1124)
                     /gene="msyB"
                     /locus_tag="OQN12_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1656024.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1150..1554)
                     /locus_tag="OQN12_003870"
     CDS             complement(1150..1554)
                     /locus_tag="OQN12_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS1656025.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            1931..3847
                     /gene="dnaK"
                     /locus_tag="OQN12_003871"
     CDS             1931..3847
                     /gene="dnaK"
                     /locus_tag="OQN12_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1656026.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3936..5066
                     /gene="dnaJ"
                     /locus_tag="OQN12_003872"
     CDS             3936..5066
                     /gene="dnaJ"
                     /locus_tag="OQN12_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1656027.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5170..5322)
                     /gene="mokC"
                     /locus_tag="OQN12_003873"
     CDS             complement(5170..5322)
                     /gene="mokC"
                     /locus_tag="OQN12_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1656028.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1400 a         1317 c         1407 g         1240 t
ORIGIN      
        1 gtttcggcat cagcagaatc gcaaccagcg tcagccagaa agaaccgtaa gaggtgaatg
       61 cggttaaacc gaaagtgttg ccttttttgt actccagcag accagcaaaa atttgcgcga
      121 tgccgccgta gaaaatgccc atggcaagaa taataccgtc cagagcgaaa taacccacgt
      181 tgtgcaggtt aagcagaatg gtggtcatgc cgaagcccat caggcccagc ggtgccggat
      241 tagccaactt agtgttgccc ataattcctc aaaaatcatc atcgaatgaa tggtgaaata
      301 atttccctga ataactgtag tgttttcagg gcgcggcata ataatcagcc agtggggcag
      361 tgtctacgat cttttgaggg gaaaatgaaa attttccccg gtttccggta tcagacctga
      421 gtggcgctaa ccatccggcg caggcaggcg atttgcagta cggctggaat cgtcacgcga
      481 taggtgctgc cgctgaccgc tttaacccca tttaatgctg cgcccacagg gcctcccagc
      541 cccgcgccgc gcagcaaacc atgcccaagt acgctcattg ctgcgtgggt gcgtaaaatg
      601 cgggtcagtt ggctggaaag caaatgcgac acaccttttg ccaataattt gtctttcatc
      661 agcagcggca gcagctcttc cagctcattc accctggcat cgaccgcgtg cagaaactcc
      721 tgcttatgtt cctcgtccat tttcttccag gtattacgca gaaattgttc cagtaactgt
      781 tgctcaattt caaacgtaga catctctttg tcggctttca gcttcaatcg ctttgaaaca
      841 tcgagcaaaa tggcccgata caatttaccg tgtccgcgca gtttgttggc gatactatcg
      901 ccaccaaaat gctgtaattc tccggcaatc agctgccagt tgcggcgatg ttgctcggga
      961 tgcccttcca tcgatttaaa cagttcgttg cgcatcagta tgctggagag gcgagttttg
     1021 cctttttcat tatgggtgag caatcgggcg aaatttgcca actgttcctc actacaatgc
     1081 tgaagaaaat ccagatctga atcattcagg taattaacat tcattttttg tggcttctat
     1141 attctggcgt tagtcgtcgc cgataatttt cagcgtggcc atatccgatg agttcaccgt
     1201 atgaccggaa aaggtgattt ttgagacgca gcgtttattg tcgttatcgc tgttaatgtt
     1261 gatccagtca gtggtttgcc cttcttttat ttctgaagga atattcaggc tctgactggc
     1321 gctacgggcg gctttgaaat aaaccgatgc accacttaac tgtaaatcgc catggtcggc
     1381 agagagttgt atgcgtttca caatgcgaca aacaggaagt ttcagcgcca gatcgttggt
     1441 ttcgttacgc ggcattgcaa tgacgccgag gagtttatgg tcgtttgcct gcgccgtgca
     1501 gcacagcatc aggctaatcg ccaggctggc ggaaatcgta aaaacggatt tcataaggat
     1561 tctcttagtg ggaagaggta gggggatgaa tacccactag tttactgctg ataaagagaa
     1621 gattcaggca cgtaatcttt tctttttatt acaatttttt gatgaacgcc ttggctgcga
     1681 ttcattcttt atatgaataa aattgctgtc aattttacgt cttgtcctgc catatcgcga
     1741 aatttctgcg caaaagcaca aaaaattttt gcatctcccc cttgatgacg tgggttacga
     1801 ccccatttag tagtcaaccg cagtgagtga gtctgcaaaa aaatgaaatt gggcagttga
     1861 aaccagacgt ttcgccccta ttacagactc acaaccacat gatgaccgaa tatatagtgg
     1921 agacgtttag atgggtaaaa taattggtat cgacctgggt actaccaact cttgtgtagc
     1981 gattatggat ggcaccattc ctcgcgtgct ggagaacgcc gaaggcgatc gcaccacgcc
     2041 ttctatcatt gcctataccc aggatggtga aactctggtt ggtcagccgg ctaaacgtca
     2101 ggcagtgacg aacccgcaaa acaccctgtt tgcgattaaa cgcctgattg gccgccgctt
     2161 ccaggacgaa gaagtacagc gtgatgtttc catcatgccg ttcaaaatta ttgctgctga
     2221 taacggcgac gcatgggtcg aagttaaagg ccagaaaatg gcaccgccgc agatttctgc
     2281 tgaagtgctg aaaaaaatga agaaaaccgc tgaagattac ctgggtgaac cggtaactga
     2341 agctgttatc accgtaccgg catactttaa cgatgctcag cgtcaggcaa ccaaagacgc
     2401 aggccgtatc gctggtctgg aagtaaaacg tatcatcaac gaaccgaccg cagctgcgtt
     2461 ggcttacggt ctggacaaag gcactggcaa ccgtactatc gcggtttatg acctgggggg
     2521 tggtactttc gatatttcta ttatcgaaat cgacgaagtt gacggcgaaa aaaccttcga
     2581 agttctggca accaacggtg atacccacct gggtggtgaa gacttcgaca gccgtctgat
     2641 caactatctg gttgaagaat tcaagaaaga tcagggcatt gacctgcgca acgatccgct
     2701 ggcaatgcag cgcctgaaag aagcggcaga aaaagcgaaa atcgaactgt cttccgctca
     2761 gcagaccgac gttaacctgc catacatcac tgcagacgcg accggtccga aacacatgaa
     2821 catcaaagtg actcgtgcga aactggaaag cctggttgaa gatctggtaa accgttccat
     2881 tgagccgctg aaagttgcac tgcaggatgc tggcctgtcc gtatctgata tcgacgacgt
     2941 tatcctcgtt ggtggtcaga ctcgtatgcc aatggttcag aagaaagttg ctgagttctt
     3001 tggtaaagag ccgcgtaaag acgttaaccc ggacgaagct gtagcaatcg gtgctgctgt
     3061 tcagggtggt gttctgactg gcgacgtaaa agacgtactg ctgctggacg ttaccccgct
     3121 gtctctgggt atcgaaacca tgggcggtgt gatgacgacg ctgatcgcga aaaacaccac
     3181 tatcccgacc aagcacagcc aggtgttctc taccgctgaa gacaaccagt ctgcggtaac
     3241 catccatgtg ctgcagggtg aacgtaaacg tgcggctgat aacaaatctc tgggtcagtt
     3301 caacctagat ggtatcaacc cggcaccgcg cggcatgccg cagatcgaag ttaccttcga
     3361 tatcgatgct gacggtatcc tgcacgtttc cgcgaaagat aaaaacagcg gtaaagagca
     3421 gaagatcacc atcaaggctt cttctggtct gaacgaagat gaaatccaga aaatggtacg
     3481 cgacgcagaa gctaacgccg aagctgaccg taagtttgaa gagctggtac agactcgcaa
     3541 ccagggcgac catctgctgc acagcacccg taagcaggtt gaagaagcag gcgacaaact
     3601 gccggctgac gacaaaactg ctatcgagtc tgcgctgact gcactggaaa ctgctctgaa
     3661 aggtgaagac aaagccgcta tcgaagcgaa aatgcaggaa ctggcacagg tttcccagaa
     3721 actgatggaa atcgcccagc agcaacatgc ccagcagcag actgccggtg ctgatgcttc
     3781 tgcaaacaac gcgaaagatg acgatgttgt cgacgctgaa tttgaagaag tcaaagacaa
     3841 aaaataatcg ccctataaac gggtaattat actgacacgg gcgaagggga atttcctctc
     3901 cgcccgtgca ttcatctagg ggcaatttaa aaaagatggc taagcaagat tattacgaga
     3961 ttttaggcgt ttccaaaaca gcggaagagc gtgaaatcag aaaggcctac aaacgcctgg
     4021 ccatgaaata ccacccggac cgtaaccagg gtgacaaaga ggccgaggcg aaatttaaag
     4081 agatcaagga agcttatgaa gttctgaccg actcgcaaaa acgtgcggca tacgatcagt
     4141 atggtcatgc tgcgtttgag caaggaggca tgggcggcgg tggttttggc ggcggcgcag
     4201 acttcagcga tatttttggt gacgttttcg gcgatatttt tggcggcgga cgtggtcgtc
     4261 aacgtgcggc gcgcggtgct gatttacgct ataacatgga gctcaccctc gaagaagctg
     4321 tacgtggcgt gaccaaagag atccgcattc cgactctgga agagtgtgac gtttgccacg
     4381 gtagcggtgc aaaaccaggt acacagccgc agacctgtcc gacctgtcat ggttctggtc
     4441 aggtgcagat gcgccaggga ttcttcgctg tacagcagac ctgtccacac tgtcagggcc
     4501 gcggtacgct gatcaaagat ccgtgcaaca aatgtcatgg tcatggtcgt gttgagcgca
     4561 gcaaaacgct gtccgttaaa atcccggcag gggtggacac tggagaccgc atccgtcttg
     4621 cgggcgaagg tgaagcgggc gagcatggcg caccggcagg cgatctgtac gttcaggttc
     4681 aggttaaaca gcacccgatt ttcgagcgtg aaggcaacaa cctgtattgc gaagtcccga
     4741 tcaacttcgc tatggcggcg ctgggtggcg aaatcgaagt accgaccctt gatggtcgcg
     4801 tcaaactgaa agtgcctggc gaaacccaga ccggtaagct attccgtatg cgcggtaaag
     4861 gcgtcaagtc tgtccgcggt ggcgcacagg gtgatttgct gtgccgcgtt gtcgtcgaaa
     4921 caccggtagg cctgaacgaa aagcagaaac agctgctgca agagctgcaa gaaagcttcg
     4981 gtggcccaac cggcgagcac aacagcccgc gctcaaagag cttctttgat ggtgtgaaga
     5041 agttttttga cgacctgacc cgctaacctc cccaaaagcc tgcccgtggg caggcctggg
     5101 taaaaatagg gtgcgttgaa gatatgcgag cacctgtaaa gtggcgggga tcactccccg
     5161 ccgttgctct tactcggatt cgtaagccgt gaaaacagca acctccgtct ggccagttcg
     5221 gatgtgaacc tcacagaggt cttttctcgt taccagcgcc gccactacgg cggtgataca
     5281 gatgacgatc agggcgacaa tcatcgcctt atgctgcttc attgctctct tctccttgac
     5341 cttacggtca gtaagaggca ctct
//
LOCUS       DAJVRP010000176         5350 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVRP010000176 DAJVRP010000000
VERSION     DAJVRP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.177"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(75..557)
                     /locus_tag="OQN12_003874"
     CDS             complement(75..557)
                     /locus_tag="OQN12_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656029.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="OQN12_003875"
     CDS             complement(625..2574)
                     /locus_tag="OQN12_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1656030.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="OQN12_003876"
     CDS             complement(2659..2994)
                     /locus_tag="OQN12_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1656031.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="OQN12_003877"
     CDS             complement(2994..3350)
                     /locus_tag="OQN12_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS1656032.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="OQN12_003878"
     CDS             complement(3350..4846)
                     /locus_tag="OQN12_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS1656033.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="OQN12_003879"
     CDS             complement(4843..5004)
                     /locus_tag="OQN12_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCS1656034.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="OQN12_003880"
     CDS             complement(5014..>5350)
                     /locus_tag="OQN12_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656035.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1276 a         1561 c         1150 g         1363 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DAJVRP010000177         5338 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVRP010000177 DAJVRP010000000
VERSION     DAJVRP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.178"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OQN12_003881"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OQN12_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1656036.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OQN12_003882"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OQN12_003882"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1656037.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OQN12_003883"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OQN12_003883"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1656038.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OQN12_003884"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OQN12_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS1656039.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1311 a         1289 c         1456 g         1282 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca acgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgttcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcgggg
//
LOCUS       DAJVRP010000178         5309 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVRP010000178 DAJVRP010000000
VERSION     DAJVRP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.179"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..146
                     /locus_tag="OQN12_003885"
     CDS             <1..146
                     /locus_tag="OQN12_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656040.1"
                     /translation="AKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVV
                     NED"
     gene            147..1205
                     /gene="rsxD"
                     /locus_tag="OQN12_003886"
     CDS             147..1205
                     /gene="rsxD"
                     /locus_tag="OQN12_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1656041.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1209..1829
                     /gene="rsxG"
                     /locus_tag="OQN12_003887"
     CDS             1209..1829
                     /gene="rsxG"
                     /locus_tag="OQN12_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1656042.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1833..2528
                     /gene="rsxE"
                     /locus_tag="OQN12_003888"
     CDS             1833..2528
                     /gene="rsxE"
                     /locus_tag="OQN12_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1656043.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            2528..3163
                     /gene="nth"
                     /locus_tag="OQN12_003889"
     CDS             2528..3163
                     /gene="nth"
                     /locus_tag="OQN12_003889"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1656044.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            3774..5276
                     /gene="dtpA"
                     /locus_tag="OQN12_003890"
     CDS             3774..5276
                     /gene="dtpA"
                     /locus_tag="OQN12_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1656045.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
BASE COUNT         1263 a         1302 c         1357 g         1387 t
ORIGIN      
        1 gcgccaaagc acgcaagctg gaacagcaac aggctaatgc ggaaccagaa gaacaggtcg
       61 atccgcgcaa agccgcagtt gccgcggcta ttgcccgcgt tcaggccaaa aaagccgccc
      121 agcaaaaggt tgtaaacgag gactaaatgg tattcagaat agctagctcc ccttataccc
      181 ataaccagcg ccagacatcg cgcattatgc tgttggtgtt gctcgcagcc gtgccaggaa
      241 tcgcagcgca actgtggttt tttggttggg gtactctcgt tcagatcctg ttggcgtcgg
      301 tcagtgctct gttagccgaa gctctcgtac tcaaactacg caagcagtcg gtagccgcaa
      361 cgttgaaaga taactcagca ttgctgacag gcttattgct ggcggtaagt attccccccc
      421 tagcgccatg gtggatggtc gtactgggta cggtgtttgc ggtgatcatc gctaaacagt
      481 tgtatggcgg tctggggcaa aacccgttta atccggcaat gattggttat gtggtcttac
      541 tgatctcctt cccggtgcag atgaccagct ggttaccgcc acatgaaatt gcggtcaaca
      601 tccctggttt tatcgacgcc atacaggtta ttttcagcgg tcataccgcc agtggtggtg
      661 atatgaatac gctacgctta ggtattgatg gcattagtca ggcgacaccg ctggatacat
      721 tcaaaacctc tgtccgcgca ggtcattcgg ttgaacagat tatgcaatat ccgatctaca
      781 gcggtattct ggcgggcgct ggttggcaat gggtaaatct cgcctggctg gctggtggcg
      841 tgtggttgct atggcagaaa gcgattcgct ggcatattcc cctcagcttc ttagtaacgc
      901 tggcgttatg cgcaacgttg ggctggttgt tctcaccaga aacactggca gcaccgcaaa
      961 ttcatctgct gtctggtgcg actatgctcg gcgcattctt tattttgact gacccagtta
     1021 ctgcttctac gaccaatcgt ggtcggctga tgttcggcgc gctggcgggc ttattagtct
     1081 ggttgatccg cagtttcggc ggctatcctg acggcgtggc ttttgccgtc ctgctggcga
     1141 acatcacggt tcctctgatc gattactaca cgcgtccgcg cgtctacggc catcgcaaag
     1201 ggtaaaccat gctgaaaact atccgaaaac acggcattac actggcacta tttgcagcgg
     1261 gttctacagg gttaactgcg gccatcaacc agatgaccaa aacgacaatt gctgaacagg
     1321 ccagtctgca acaaaagacg ttatttgatc aggtgctgcc agccgaacgc tataacaatg
     1381 cgctcgcaca gagttgctat ctggtaactg cgccagagtt aggtaaaggt gagcatcggg
     1441 tttacatcgc caaacaggat gacaaaccgg tagccgccgt tctggaagca accgcgccag
     1501 atggctattc cggtgcgatt cagctgctgg tgggagccga ttttaatggc acggtacttg
     1561 gcacgcgcgt gacagagcac cacgaaacgc cagggcttgg cgataaaatc gaattgcgcc
     1621 tttctgactg gctcacccat tttgcgggaa aaaaaatcag tggtgcggat gatgcgcact
     1681 gggcggtgaa gaaagatggt ggtgatttcg accagttcac cggcgcgacg attactcccc
     1741 gcgcggtggt taatgcggta aaacgcgccg gattgtacgc tcagacgtta ccggcacaac
     1801 tttctcaact tcctgcctgt ggagaataaa gcgtgagcga aattaaagac gttattgttc
     1861 aggggttgtg gaaaaacaac tctgcactgg tccaactgct cggcctttgt ccgctattgg
     1921 cggtcacatc cactgccact aacgctctgg gtttaggact tgcgactacg ctggtactga
     1981 cgctgaccaa cctgaccatt tcgacgctgc gtcactggac gccagccgag atccgcattc
     2041 ccatttacgt gatgatcatc gcctcggtgg tcagcgctgt acagatgctg atcaacgcct
     2101 acgcctttgg cctgtatcaa tcattaggga tttttattcc gctgattgtc actaactgta
     2161 tcgttgtggg ccgcgctgaa gccttcgccg ccaaaaaagg tccggcgctt tcggcactgg
     2221 acggcttttc aattggtacg ggcgcaacct gcgccatgtt cgtgctgggt tcactacgcg
     2281 aaattatcgg caatggcacg ttgtttgacg gtgcagatgc gctgttaggt agctgggcaa
     2341 aagtattacg cgtggagatt ttccacaccg actccccttt cctgctggcg atgctgccac
     2401 caggtgcatt tattggcttg ggactgatgc tggcaggaaa atacctgatt gatgaaagaa
     2461 tgaaaaagcg ccgtgctgaa gcagctgccg aacgtgcatt gccaaacggt gaaacaggga
     2521 atgtctgatg aacaaagcaa aacgtctgga gatcctcact cgcctgcgtg agaacaatcc
     2581 tcatcccacc accgagctta atttcagttc gccttttgaa ttgctgattg ccgtactgct
     2641 ttccgctcag gcgaccgata tcagtgttaa taaggcgacg gcgaaactct acccggtggc
     2701 gaatacgcct gcagcgatgc ttgaactagg cgttgaaggg gtgaaaacct atatcaaaac
     2761 gattggcctt tataacagca aagcagaaaa tatcatcaaa acctgccgta tattactgga
     2821 acaacataat ggcgaggttc cggaagatcg tgctgcgctt gaagccctgc ccggcgtagg
     2881 tcgtaaaaca gccaacgtcg tattaaacac tgcattcggc tggccgacta ttgctgtcga
     2941 cacgcacatt ttccgcgttt gtaatcgtac tcaatttgcg ccggggaaaa acgtcgaaca
     3001 ggtagaagaa aagctactga aagtggttcc ggcagagttt aaagtcgact gccaccattg
     3061 gttgatcctg cacgggcgtt atacctgcat tgcccgcaag ccccgctgtg gctcttgtat
     3121 tattgaagat ctttgtgaat acaaagagaa agttgacatc tgaagaaaag gggtaacacc
     3181 gattacccca ttgataacct ttctttatcc tcttttaaaa catcttttta acgtcaatga
     3241 tgccattgct tagcgttatc atcaggtaat ccgtttgcgg ataaccaaaa atgcaggtta
     3301 attgtttttt taatagcgaa attttctatt cattcgtgat caagatcacg atgctgtggc
     3361 aatgtaaaaa cattgcaaga atcaggttaa aatgatttaa atttacacac gcaacaaata
     3421 ttgacctgca aaacattaca ctggctattt ttcagaaact ggactatctc actaggaaaa
     3481 acgctaaata gcagaacata tcgccgccaa gccatcatcc ccctctcaat gcagtgaaaa
     3541 aatctgccgt tacgtttttt gaaaaattta acgctggata acatttcccg gaatggttga
     3601 attccccgcc tcagttatat gtaacagatt attacaaagg acttgtctga aagtgcaaga
     3661 tagtgaacat tacctgccgt ttcccctccc actataacaa ttgcgcgtat gttttttaga
     3721 cataacgcga gaaagcaccc ccgttaatat gggatgtaaa aaaagaggta aaagtgtcca
     3781 ctgcaaacca aaaaccaact gaaagcgtca gtttgaacgc tttcaaacaa ccgaaggcgt
     3841 tctatctcat cttctcgatt gagttatggg aacgttttgg ttattacggc ctacaaggaa
     3901 ttatggctgt ttacctggtt aaacaactgg gtatgtctga agcggattca atcacccttt
     3961 tctcttcctt tagtgccctg gtttatggtc tggtcgctat cggcggctgg ttaggtgaca
     4021 aggtactggg tactaaacgc gtaattatgc tcggcgctat tgtgctggcg attggttatg
     4081 ctctggttgc ctggtctggt cacgacgccg gtattgttta tatgggtatg gcggctattg
     4141 cggtcggtaa cggcctgttt aaagctaacc cgtcttctct gctttctaca tgctatgaga
     4201 aaaacgaccc acgtctggac ggtgcattca ccatgtacta catgtccgtc aacatcggct
     4261 ctttcttctc tatgatagcc accccgtggc tggccgcgaa atacggctgg agtgttgcgt
     4321 ttgcgttgag cgttgtaggc ctgctgatca ctatcgttaa cttcgccttc tgccaacgct
     4381 gggttaaaca gtacggttca aaaccagact ttgaaccagt caactaccgt aacctgttgc
     4441 tgaccattat tggtgttgtg gcgctgatcg ctgttgccac ctggctactg cacaatcagg
     4501 aagttgcgcg tatggcgctg ggcgttgttg ccttcggtat tgtggttatc ttcggtaaag
     4561 aagctttcgc gatgaaaggt gctgcgcgtc gtaaaatgat cgttgccttc atcctgatgc
     4621 tcgaagcaat catcttcttc gtgctgtaca gccagatgcc aacgtcactg aacttctttg
     4681 cgattcgtaa cgttgagcac tccattctgg gtctggccgt agaacctgag cagtatcagg
     4741 cactgaaccc gttctggatc atcatcggta gtccgattct ggccgctatc tataacaaga
     4801 tgggtgatac cctgccgatg ccaaccaagt ttgcaatcgg catggtgatg tgttctggtg
     4861 cgttcctgat tctgccgctg ggtgcgaaat tcgcgtctga cgcaggtatc gtgtctgtca
     4921 gctggctggt cgcaagctat ggcctgcaga gcatcgggga actgatgatc tctggtctgg
     4981 gtctggcaat ggttgcacaa ctcgttccgc agcgtctgat gggcttcatt atgggtagct
     5041 ggttcctgac cactgccggt gcaaacctga ttggtggtta tgttgcgggt atgatggctg
     5101 tgccggataa cgttaccgac ccgctgatgt cactggaagt ctatggtcga gtattcttgc
     5161 agattggtgt cgctactgcc gttattgcag tactgatgct gctgaccgcg cctaaactgc
     5221 accgcatgac gcaggatgac gctgcagaca aagcggcgaa agctactgta gcgtaaaatt
     5281 cagggaaact cttttacaag ccgctaact
//
LOCUS       DAJVRP010000179         5293 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVRP010000179 DAJVRP010000000
VERSION     DAJVRP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.180"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..331
                     /locus_tag="OQN12_003891"
     CDS             <1..331
                     /locus_tag="OQN12_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCS1656046.1"
                     /translation="KTLGNKGIGLEGGGIFTVGDECHAHFLVEQGKEAKIALEQAGLL
                     VLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYSDHANQLILITDNDSMAASV
                     TLPWAIK"
     gene            339..1025
                     /locus_tag="OQN12_003892"
     CDS             339..1025
                     /locus_tag="OQN12_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1656047.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1003..1626)
                     /gene="tetR(B)"
                     /locus_tag="OQN12_003893"
     CDS             complement(1003..1626)
                     /gene="tetR(B)"
                     /locus_tag="OQN12_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1656048.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1708..2913
                     /gene="tet(B)"
                     /locus_tag="OQN12_003894"
     CDS             1708..2913
                     /gene="tet(B)"
                     /locus_tag="OQN12_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1656049.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3026..3619)
                     /gene="tetC"
                     /locus_tag="OQN12_003895"
     CDS             complement(3026..3619)
                     /gene="tetC"
                     /locus_tag="OQN12_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCS1656050.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3752..4123
                     /locus_tag="OQN12_003896"
     CDS             3752..4123
                     /locus_tag="OQN12_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1656051.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4133..>5293)
                     /locus_tag="OQN12_003897"
     CDS             complement(4133..>5293)
                     /locus_tag="OQN12_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCS1656052.1"
                     /translation="ELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKR
                     IDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHG
                     RSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLG
                     WYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKS
                     RSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIE
                     ETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQA
                     NTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1441 a          990 c         1159 g         1703 t
ORIGIN      
        1 aaaaacatta ggtaataaag gtattggatt ggaaggggga gggatattta cggttggtga
       61 tgaatgccat gctcattttc ttgttgaaca aggaaaggaa gctaaaatag cgctagagca
      121 agctggactg ttagtacttg cgatccggac accattaatt cgtaagttaa aacaggaaaa
      181 accgggggaa cttggcgaaa tagcacgagt attggcggag aataacatta atattttagt
      241 gcaatacagt gaccatgcta accaactgat attaataacg gacaatgata gtatggctgc
      301 atctgttacg ctcccttggg caataaagtg aacttgcgat ggctaattta atacgaaaag
      361 aggttacctt tgagtcctca atagccgcga taggggcggc tatgtctgac atttcacgag
      421 ttaaaatact cagtgctttg atggatgggc gagcttggac ggccactgag ctaagttctg
      481 tggcgaatat atcagcttca acggcgagca gtcatttatc taaattatta gattgccagc
      541 taatcacagt agtagctcaa ggcaagcatc gttattttcg gctagcagga aaagatattg
      601 ctgaattgat ggaaagtatg atggggatct ccttaaacca tggcgtacat gccaaagttt
      661 ccacgccagt gcatttacga aaagcacgta cttgctatga tcatttagct ggcgaagttg
      721 ccgttaagat ctatgattcc ctttgtcaac agcaatggat cactgaaaat ggttcaatga
      781 tcacattaag tggtattcaa tattttcatg aaatgggaat tgacgttcct tccaaacatt
      841 cacgtaaaat ctgttgtgcg tgtttagatt ggagtgaacg ccgtttccat ttaggtgggt
      901 acgttggagc cgcattattt tcgctttatg aatctaaagg gtggttaact cgacatcttg
      961 gttaccgtga agttaccatc acggaaaaag gttatgctgc ttttaagacc cactttcaca
     1021 tttaagttgt ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc
     1081 tgcaccttgg tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt
     1141 agtaggtgtt tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc
     1201 taaagtaaaa tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg
     1261 ttggcataaa aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt
     1321 acctaaatgt acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc
     1381 gttattacgt aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg
     1441 cctatctaac atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca
     1501 atacaatgta ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc
     1561 gattccgacc tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct
     1621 agacatcatt aattcctaat ttttgttgac actctatcat tgatagagtt attttaccac
     1681 tccctatcag tgatagagaa aagtgaaatg aatagttcga caaagatcgc attggtaatt
     1741 acgttactcg atgccatggg gattggcctt atcatgccag tcttgccaac gttattacgt
     1801 gaatttattg cttcggaaga tatcgctaac cactttggcg tattgcttgc actttatgcg
     1861 ttaatgcagg ttatctttgc tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc
     1921 ccagtgctgt tgttgtcatt aataggcgca tcgctggatt acttattgct ggctttttca
     1981 agtgcgcttt ggatgctgta tttaggccgt ttgctttcag ggatcacagg agctactggg
     2041 gctgtcgcgg catcggtcat tgccgatacc acctcagctt ctcaacgcgt gaagtggttc
     2101 ggttggttag gggcaagttt tgggcttggt ttaatagcgg ggcctattat tggtggtttt
     2161 gcaggagaga tttcaccgca tagtcccttt tttatcgctg cgttgctaaa tattgtcgct
     2221 ttccttgtgg ttatgttttg gttccgtgaa accaaaaata cacgtgataa tacagatacc
     2281 gaagtagggg ttgagacgca atcgaattcg gtatacatca ctttatttaa aacgatgccc
     2341 attttgttga ttatttattt ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg
     2401 gtgctattta ccgaaaatcg ttttggatgg aatagcatga tggttggctt ttcattagcg
     2461 ggtcttggtc ttttacactc agtattccaa gcctttgtgg caggaagaat agccactaaa
     2521 tggggcgaaa aaacggcagt actgctcgga tttattgcag atagtagtgc atttgccttt
     2581 ttagcgttta tatctgaagg ttggttagtt ttccctgttt taattttatt ggctggtggt
     2641 gggatcgctt tacctgcatt acagggagtg atgtctatcc aaacaaagag tcatcagcaa
     2701 ggtgctttac agggattatt ggtgagcctt accaatgcaa ccggtgttat tggcccatta
     2761 ctgtttgctg ttatttataa tcattcacta ccaatttggg atggctggat ttggattatt
     2821 ggtttagcgt tttactgtat tattatcctg ctatcgatga ccttcatgtt aacccctcaa
     2881 gctcagggga gtaaacagga gacaagtgct tagttatttc gtcaccaaat gatgttattc
     2941 cgcgaaatat aatgaccctc ttgataaccc aagagggcat tttttacgat aaagaagatt
     3001 tagcttcaaa taaaacctat ctattttatt tatctttcaa gctcaataaa aagccgcggt
     3061 aaatagcaat aaattggcct tttttatcgg caagctcttt taggtttttc gcatgtattg
     3121 cgatatgcat aaaccagcca ttgagtaagt ttttaagcac atcatcatca taagctttaa
     3181 gttggttctc ttggatcaat ttgctgacaa tggcgtttac cttaccagta atgtattcaa
     3241 ggctaatttt ttcaagttca ttccaaccaa tgataggcat cacttcttgg atagggataa
     3301 ggtttttatt attatcaata atataatcaa gataatgttc aaatatactt tctaaggcag
     3361 accaaccatt tgttaaatca gtttttgttg tgatgtaggc atcaatcata attaattgct
     3421 gcttataaca ggcactgagt aattgttttt tatttttaaa gtgatgataa aaggcacctt
     3481 tggtcaccaa cgcttttccc gagatctcat ctattgaaac agcttgatag cctttttcaa
     3541 caaacaatat tcgtgctgag ttaaccagtg attgataggt actcttaaaa ttttcttgtt
     3601 gatgattttt attttccatg atagatttaa aataacatac cgtcagtatg tttatggtat
     3661 catgatgatg tggtcgtgac aatcttaaga acatttaggt tattttatgt atattgaaca
     3721 gcattctcgc tatcaaaata aagctaataa catccaatta agatatgatg ataagcagtt
     3781 tcatacaacg gttatcaaag atgttctatt atggattgaa cataatttag atcagtcttt
     3841 actgcttgat gatgtggcga ataaagcggg ttataccaag tggtattttc agcggctgtt
     3901 caaaaaagta acaggggtca cactggctag ctatattcgt gctcgtcgtt tgacgaaagc
     3961 ggctgttgag ttgaggttga cgaaaaaaac tatccttgag atcgcattaa aatatcaatt
     4021 tgattcccaa caatctttta cacgtcgatt taagtacatt tttaaggtta caccaagtta
     4081 ttatcggcgt aataaattat gggaattgga ggcaatgcac tgagagatcc cctcataatt
     4141 tccccaaagc gtaaccatgt gtgaataaat tttgagctag tagggttgca gccacgagta
     4201 agtcttccct tgttattgtg tagccagaat gccgcaaaac ttccatgcct aagcgaactg
     4261 ttgagagtac gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt
     4321 tctgagcatg aacgcccgca agccaacatg ttagttgaag catcagggcg attagcagca
     4381 tgatatcaaa acgctctgag ctgctcgttc ggctatggcg taggcctagt ccgtaggcag
     4441 gacttttcaa gtctcggaag gtttcttcaa tctgcattcg cttcgaatag atattaacaa
     4501 gttgtttggg tgttcgaatt tcaacaggta agttagttgc tagaacccat ggctcctttg
     4561 ccgacgctga gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat
     4621 tttttcggcc tttagagcga gatttataca atagaatttg gcatgagatt ggattgcttt
     4681 tagtcagcct cttatagcct aaagtctttg agtgactaga tgacatatca tgtaagttgc
     4741 tgataggttt ccagttttcc gctcctaggt ctgcatattg tacttttcct cttactcgac
     4801 ttaaccagta ccaacccagc ttctcaacgg atttatacca tggcacttta aagccagcat
     4861 cactgacaat gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt
     4921 ggtcatgagc tttctttgaa cattgctctg aaagcgggaa cgctttctca taaagagtaa
     4981 cagaacgacc gtgtagtgcg actgaagctc gcaataccat aagtcgtttt tgctcacgaa
     5041 tatcagacca gtcaacaagt acaatgggca tcgtattgcc cgaacagata aagctagcat
     5101 gccaacggta tacagcgagt cgctctttgt ggaggtgacg attacctaac aatcggtcga
     5161 ttcgtttgat gttatgtttt gttctcgctt tggttggcag gttacggcca agttcggtaa
     5221 gagtgagagt tttacagtca agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt
     5281 ttaagtgtaa ttc
//
LOCUS       DAJVRP010000180         5163 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVRP010000180 DAJVRP010000000
VERSION     DAJVRP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.181"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..221
                     /locus_tag="OQN12_003898"
     CDS             <1..221
                     /locus_tag="OQN12_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1656053.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(393..722)
                     /locus_tag="OQN12_003899"
     CDS             complement(393..722)
                     /locus_tag="OQN12_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCS1656054.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(727..912)
                     /locus_tag="OQN12_003900"
     CDS             complement(727..912)
                     /locus_tag="OQN12_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1656055.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(909..3548)
                     /locus_tag="OQN12_003901"
     CDS             complement(909..3548)
                     /locus_tag="OQN12_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1656056.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3756..4745
                     /gene="ldhA"
                     /locus_tag="OQN12_003902"
     CDS             3756..4745
                     /gene="ldhA"
                     /locus_tag="OQN12_003902"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1656057.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4856..>5163
                     /gene="hslJ"
                     /locus_tag="OQN12_003903"
     CDS             4856..>5163
                     /gene="hslJ"
                     /locus_tag="OQN12_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCS1656058.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1305 a         1322 c         1205 g         1331 t
ORIGIN      
        1 gtttgtaccg aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc
       61 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
      121 tcggctttca ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct
      181 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta
      241 ctgcaacgcc aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
      301 tttgacagga gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc
      361 tgtagcgtta tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt
      421 atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc
      481 gcgatatcat ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt
      541 tcggcattaa tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga
      601 taaccataaa atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
      661 attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc
      721 atcgcctcag aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt
      781 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc
      841 agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag
      901 taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga
      961 cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
     1021 gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg
     1081 cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt
     1141 tttgtcgatg accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc
     1201 atattgtctt tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattggcccg
     1261 ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat
     1321 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
     1381 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
     1441 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct
     1501 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     1561 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     1621 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     1681 gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca
     1741 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
     1801 accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga
     1861 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
     1921 ttaaagagga aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt
     1981 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
     2041 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     2101 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     2161 atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta
     2221 aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     2281 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     2341 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     2401 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
     2461 tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat
     2521 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc
     2581 atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg
     2641 gtcagcacat tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg
     2701 actttgacac ccagtcggcc actcaaagga aaacctgcat acagccagct ccagcgacca
     2761 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
     2821 ttatcccgtg ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc
     2881 accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt
     2941 ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     3001 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     3061 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
     3121 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
     3181 ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
     3241 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     3301 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     3361 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     3421 cgtgtcccga gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc
     3481 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
     3541 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     3601 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
     3661 tttttcaata tcgccatggc tttcaattat atttgaaatt ttgtaaaata tttttagtag
     3721 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
     3781 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
     3841 tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat
     3901 gtattttcgt aaacgatgac ggcagccacc cggtgctgga agagctgaaa aagcacggcg
     3961 ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag
     4021 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg
     4081 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
     4141 acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg
     4201 ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
     4261 gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg
     4321 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
     4381 cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga
     4441 tcgtcaatac cagtcgcggt gcattgattg attctcaggc ggcaattgaa gcgctgaaaa
     4501 atcagaaaat tggttcgttg ggtatggacg tctatgagaa cgaacgcgat ctgttctttg
     4561 aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct gcctgccaca
     4621 acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc
     4681 agactacgct gcaaaactta agcaatctgg aaaaaggtga aacctgcccg aacgaactgg
     4741 tttaatcttg cagctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct
     4801 ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa
     4861 gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
     4921 aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg
     4981 taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat
     5041 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
     5101 agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
     5161 taa
//
LOCUS       DAJVRP010000181         5156 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVRP010000181 DAJVRP010000000
VERSION     DAJVRP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.182"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            188..445
                     /locus_tag="OQN12_003904"
                     /pseudo
     CDS             188..445
                     /locus_tag="OQN12_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="OQN12_003905"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="OQN12_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1656059.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="OQN12_003906"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="OQN12_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1656060.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="OQN12_003907"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="OQN12_003907"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1656061.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="OQN12_003908"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="OQN12_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3603..4418)
                     /locus_tag="OQN12_003909"
     CDS             complement(3603..4418)
                     /locus_tag="OQN12_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1656062.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5156
                     /locus_tag="OQN12_003910"
     CDS             4515..>5156
                     /locus_tag="OQN12_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1656063.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVH"
BASE COUNT         1376 a         1175 c         1226 g         1379 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccctt gttcac
//
LOCUS       DAJVRP010000182         5023 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVRP010000182 DAJVRP010000000
VERSION     DAJVRP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.184"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            47..121
                     /gene="tnaC"
                     /locus_tag="OQN12_003911"
     CDS             47..121
                     /gene="tnaC"
                     /locus_tag="OQN12_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCS1656064.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            342..1757
                     /gene="tnaA"
                     /locus_tag="OQN12_003912"
     CDS             342..1757
                     /gene="tnaA"
                     /locus_tag="OQN12_003912"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCS1656065.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1848..3095
                     /gene="tnaB"
                     /locus_tag="OQN12_003913"
     CDS             1848..3095
                     /gene="tnaB"
                     /locus_tag="OQN12_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCS1656066.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3227..4402
                     /gene="mdtL"
                     /locus_tag="OQN12_003914"
     CDS             3227..4402
                     /gene="mdtL"
                     /locus_tag="OQN12_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1656067.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4377..>5023
                     /gene="yidZ"
                     /locus_tag="OQN12_003915"
     CDS             4377..>5023
                     /gene="yidZ"
                     /locus_tag="OQN12_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS1656068.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1145 a         1209 c         1255 g         1414 t
ORIGIN      
        1 ctgagtgtaa taatgtagcg gcgtgtcttt cgaggataag tgcattatga atatcttaca
       61 tgtatgtgtg acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta
      121 atttaccctt ctgtagccat caccagagcc aaaccgatta gattcaatgt gatcgattag
      181 tttgctatat cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta
      241 gtaaatgatg gtgcttgtat atatacatgg tgaattaatg gatattgcag atgtaatatt
      301 cacagggatc actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc
      361 tccctgaacc gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc
      421 gtaaagaagc aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt
      481 ttatcgattt actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga
      541 tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag
      601 tgaaaaatat ctttggttat caatatacta ttccgactca ccagggccgt ggcgcagagc
      661 aaatctatat tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca
      721 aaatggtggc attctctaac tatttctttg ataccacgca gggccatagc cagattaacg
      781 gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg cgttacgact
      841 ttaaaggcaa ctttgacctc gaaggattag aacgcggtat tgaagaagtt ggcccgaata
      901 acgtgccgta tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac
      961 tggcaaactt aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg
     1021 actccgcacg ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag
     1081 actggaccat cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt
     1141 ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct
     1201 tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat
     1261 atggcggcct ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga
     1321 atctcgactg gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag
     1381 agattggcgt tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac
     1441 tgctgccgca tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata
     1501 aagtcgccgg tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa
     1561 ccggtaaaca actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat
     1621 atactcaaac acatatggac ttcattattg aagcctttaa acatgtaaaa gagaacgcgg
     1681 cgaatattaa aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa
     1741 aactgaaaga agtttaatta atactacaga gtggctataa ggatgttagc cactctctta
     1801 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag
     1861 ctgaaaaaaa gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg
     1921 gaggtatgtt tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta
     1981 tccttatcat tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt
     2041 taaattatcc tgtcggctcc agttttaaca ccatcaccaa agatttaata ggtaacacct
     2101 ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
     2161 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
     2221 atccacgtat tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt
     2281 cgttagccgc cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga
     2341 tcgtgtttgg ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca
     2401 ccactgcggg aacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2461 catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata
     2521 agttaatcaa aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc
     2581 tctattgcac gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg
     2641 gcaacattga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt
     2701 tttgcctgct ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2761 ggttgttcga ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca
     2821 aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct
     2881 ttatttacgg gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg
     2941 cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
     3001 gcaatcttat tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg
     3061 gcaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc
     3121 tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct tatcctcctt
     3181 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
     3241 gatttgtagt tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg
     3301 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
     3361 cgtatatctg gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg ccgatcgttc
     3421 agggagaaag ccggtcgcca tacccggcgc ggcgctattt attattgcct cggtgttctg
     3481 ttcactggct gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc
     3541 aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
     3601 ggctaaagtg ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt
     3661 gctcggacat ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat
     3721 gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc
     3781 ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc gttttttcct
     3841 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc
     3901 gccggtattg ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca ccattatggc
     3961 gctgaccgct ggcgtcagca tgaccttttc attctcgacg ccatttgcgc tgggaatttt
     4021 taagccacgt acgttgatga tcacctcgca ggtgttattc cttgcggcgg ggatcaccct
     4081 tgccgtttca ccttcccatg cggtttctct gtttggtatc acgctgattt gcgccggttt
     4141 ctcggtaggt tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg
     4201 cgtagccagc tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct
     4261 ggccgcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
     4321 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga
     4381 agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg
     4441 agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt
     4501 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
     4561 tgtcgcccac acagctaatg gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga
     4621 gcaatctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagttgg
     4681 cggcggaatc gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
     4741 gttacccgca ggcgaccatc aaactacgta actgggatta cgattcctta gatgccatta
     4801 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc
     4861 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtaccctgcg
     4921 tctggttacg caaagatcat ccggcactgc atgaaacgtg gaatctggac acctttttac
     4981 gttatccgca tatcagcatt tgctgggaac agagcgacac ctg
//
LOCUS       DAJVRP010000183         5010 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVRP010000183 DAJVRP010000000
VERSION     DAJVRP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.185"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(684..1100)
                     /locus_tag="OQN12_003916"
     CDS             complement(684..1100)
                     /locus_tag="OQN12_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656069.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1127..1267
                     /locus_tag="OQN12_003917"
                     /pseudo
     CDS             1127..1267
                     /locus_tag="OQN12_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1267..2307
                     /locus_tag="OQN12_003918"
     CDS             1267..2307
                     /locus_tag="OQN12_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1656070.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2335..2424)
                     /locus_tag="OQN12_003919"
     tRNA            complement(2335..2424)
                     /locus_tag="OQN12_003919"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2388..2390),aa:Ser,seq:cga)
     gene            2518..3315
                     /gene="mtfA"
                     /locus_tag="OQN12_003920"
     CDS             2518..3315
                     /gene="mtfA"
                     /locus_tag="OQN12_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1656071.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3416..3491
                     /locus_tag="OQN12_003921"
     tRNA            3416..3491
                     /locus_tag="OQN12_003921"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3449..3451,aa:Asn,seq:gtt)
     gene            3653..4915
                     /locus_tag="OQN12_003922"
                     /pseudo
     CDS             3653..4915
                     /locus_tag="OQN12_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1383 a         1179 c         1123 g         1325 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
       61 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      121 tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
      241 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
      301 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
      361 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
      421 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
      481 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
      541 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
      601 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
      661 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
      721 ttaatctggt tgccggatgc tggtggattt tcgcgtttta attgttcata aaagtgcaca
      781 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
      841 tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc
      901 cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc
      961 ggctctgctt ccagcgatgc ccgtgcaatt ttgaataact cgccctctac tcgcgccatc
     1021 cctgaattgg ggtggcattt cgtaatcgct atttttaatt tggcttcttc gattaattgt
     1081 tcttttgtta attcagtcat tttcattacc gccctggtaa tgctgccacc agcgaaaatg
     1141 gttaatggaa aacgggcttc cgttctatac caatcgtagt ggcaaaccga ttgtcagccg
     1201 ggatctattt acctgcaata aaactttacc accacgcgag gtagagccga attttggtgc
     1261 gatctgatgg gaagacgaag gaaaaatcct gaacacgaaa aattacctcc aaatgtatac
     1321 ccaaataaat atagttatgt atggaaacca acatccagag aatctgtaac actaaccgcc
     1381 atcaaggatg gtttagctgc tttatggaaa aagtatgagg aaactgtaaa taatcgcgat
     1441 cgtgcaatga cattcggtcg cttgtgggaa aaattcctcg ccagcgccta ttacagtgac
     1501 ctcagtccaa gaacacaaaa agattatctg caacatcaaa aaaagttgct tgccgtattc
     1561 ggtaaggtgc cggcagattc cataaaacca gaacacatcc gtcgatacat ggacaagaga
     1621 ggggaacaga gtaaaacgca agccaaccat gaaaaaagca gtatgtcccg cgtttacagt
     1681 tgggggtatg agcgagggta cgtgaaggct aacccatgtg caggtgtaag taaattcaag
     1741 gccaaaaacc gcgaacgata tgtaaccgac aaagaatacc aggcagtatt aagcgttgca
     1801 cctcttcctg tttttatcgc aatggaaatt gcctatctgt gtgcagcgag ggtttccgat
     1861 gtgttatcgc tgaaatggga gcagattgga aacgacggga tctttatcca gcaagggaaa
     1921 acaggaaaaa aacagataaa agcatggagt ccacgattac aggcggcgat cgaaaaagca
     1981 aaacagttac caacatccgc ctatgtaatc agcaatcaat acggcaaccg atatatgtac
     2041 aaaggcttta acgaaatgtg ggtagaagca agaaatcgcg caggcaaaat ttcaggtatt
     2101 ttaaccgact tcacctttca tgatctgaag gcgaaaggaa tttcagacta tgaaggaagc
     2161 agtcgggata agcaactttt ctctggtcac aaaaccgagg ggcaagtgct aatctatgac
     2221 aggaaggtta aagtttcacc gacacttgat gtcccgttac ctgaaaatat tccaagaaaa
     2281 tattccaagt gtgatttttg tcactgactt aatgatgtat aagtgattga attttggcgg
     2341 agagaggggg atttgaaccc ccggtggagt tgcccccact ccggttttcg agaccggtcc
     2401 gttcagccgc tccggcatct ctccgttcag atggttgtca tgatgccagg aaatttggca
     2461 ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag caaaacgatg
     2521 attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa
     2581 gcactatcga tccccctttt aacgtgtctg acagaacagg aacaaagcaa attggtcgct
     2641 cttgccgaac gttttttaca gcaaaaacgg cttgttcctt tacagggctt tgagctgaat
     2701 tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga gttaggactg
     2761 gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt ggtcgatgat
     2821 gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc aggtcagagc
     2881 tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt tgatgcttct
     2941 ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa cggcgatcgc
     3001 gccagcggag ttccctttat ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat
     3061 gctgcaatga acaacattca ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt
     3121 gatgcttatg ttgccagtga tcctgctgaa tgttttgccg tactttctga atatttcttt
     3181 agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt
     3241 tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc gttttcagcg
     3301 acgaatgttc attaattaac aactttgaag attaattaac caattgaaat ggcttatgaa
     3361 atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc acgattcctc
     3421 tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt
     3481 cagaggagcc aattttctgt tttcatgcat ccttgcgaat ctttatctga tgttgattca
     3541 acaggttagt gtgaaaactc ttctcggtgc attttgattt taccctacgc atcgggaaaa
     3601 attggtggtc aaatctggga tcaggttagt tcgataatgg agtgaccccc atatatccct
     3661 taccgacgca aaaatccgca ccctcaagcc ttctgataaa ccctttaaag tctccgattc
     3721 tcacggtctg tatctgctgg tcaagccggg tggctcccgc cactggtatc tcaaataccg
     3781 tattagcggt aaagaatccc gcattgcgct gggtgcctat ccagccatct ccctgtctga
     3841 tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg ctgaatatca acccggtaca
     3901 gcagcgggct gctgaacgtg gctcacgaac accggagaaa gtttttaaaa acatggcgct
     3961 ggcgtgacat aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag
     4021 cctgaacaat cacatctttc cggtcatcgg gaacctacct gtatcagaac ttaaaccccg
     4081 tcatttcatt gacctgctga aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg
     4141 cacacggcag cacctgagta acataatgcg ccatgcggtc catcaggagt taatcgatac
     4201 gaaccctgca gcaaaccttg gcggcgtgac cacacctcct gtcagacggc actatcctgc
     4261 cctgccgctg gagcggctgc ctgaactgct tgaacgtatt ggggcatatc atcagggccg
     4321 tgaactgacc cggcatgccg ttctgctgat gctgcatgtg ttcattcgct ccagtgaact
     4381 gcgtttcgcc cgctggtcag agattgattt cacaaaccga gtctggacga tacccgcgac
     4441 gcgagaaccc attattggcg tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca
     4501 tatcgtcccc ctctcagaac agtccatcgc cattctgaaa cagattaagg atatcaccgg
     4561 taataatgaa ctgatcttcc ccggcgacca taacccgtat aagccaatgt gtgaaaacac
     4621 ggtcaataag gcactgcggg tgatgggtta cgacacgaaa aaggatatct gcggtcacgg
     4681 cttccgggca atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt
     4741 agaacgccag atgagtcatc aggagcgcaa taccgtgcgc atggcttata ttcataaggc
     4801 agagcacctg gaagcccgca aagcgatgat gcagtggtgg tcggattatc tggaagcatg
     4861 ccgagaatct tatgcaccgc cttatacaat tggtaaaaat aagtttatcc catagtatac
     4921 taacgaatcc cacactctgg caaattacca ccacctctac aggaggtggt tctatataga
     4981 cttttatcaa cttcatgacg aaaaacacct
//
LOCUS       DAJVRP010000184         4872 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVRP010000184 DAJVRP010000000
VERSION     DAJVRP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.186"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OQN12_003923"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OQN12_003923"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCS1656072.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="OQN12_003924"
     CDS             1020..1703
                     /locus_tag="OQN12_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCS1656073.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="OQN12_003925"
     CDS             1700..2161
                     /locus_tag="OQN12_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCS1656074.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="OQN12_003926"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="OQN12_003926"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1656075.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="OQN12_003927"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="OQN12_003927"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1656076.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OQN12_003928"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="OQN12_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCS1656077.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OQN12_003929"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="OQN12_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCS1656078.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1131 a         1287 c         1228 g         1226 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ctgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt atccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc ta
//
LOCUS       DAJVRP010000185         4867 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVRP010000185 DAJVRP010000000
VERSION     DAJVRP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.187"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..970
                     /locus_tag="OQN12_003930"
     CDS             <1..970
                     /locus_tag="OQN12_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1656079.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1551..2774
                     /locus_tag="OQN12_003931"
     CDS             1551..2774
                     /locus_tag="OQN12_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656080.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3021..3587
                     /gene="slp"
                     /locus_tag="OQN12_003932"
     CDS             3021..3587
                     /gene="slp"
                     /locus_tag="OQN12_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1656081.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3743..4273
                     /locus_tag="OQN12_003933"
     CDS             3743..4273
                     /locus_tag="OQN12_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1656082.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4315..4867)
                     /locus_tag="OQN12_003934"
                     /pseudo
     CDS             complement(4315..4867)
                     /locus_tag="OQN12_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1556 a          999 c          905 g         1407 t
ORIGIN      
        1 cgcagagatg tttgtgtttc tcgccgtcga gctttcgttg ctgtttatag tgattagtgc
       61 cggtgtcagc ctgataagac aaaaggtgcc agaccataaa atccagcaga tgatgggggc
      121 cagaaaaggg agaggctatc tcctggctgc tctgttggga gccgttaccc cgttctgtag
      181 ttgctcgaca atacccatgt tacgtggatt gttgtcagcg aaagccgggt ttggtccgac
      241 tctcactttt ttatttgttt caccattact taatcccatt atcgtcggac taatgtggat
      301 gacctttggt tggaaagtca ccttgttgta cgcgattatc gcagccggcg tctccgttct
      361 tgccagtatt atcctggatt ctctggggtt tgaacgtcat atcattgcca gtaaaagctc
      421 atcagcaaat tgttgtgctc cagcaaaaac ttcgccggga acgacatata cgcccataaa
      481 agtgagttgt tgtagtccag cggctaaagc cattgagaac cccgtagtta cctgttgcag
      541 taccaaagct gtagtaagta ttaatcccat aaaactagcc accaaagatg cgttgcaaca
      601 gtttaaagat gtactgccat atcttttgtt aggagtatta ataggatctt ttatttatgg
      661 ctttattccc tcagcgtgga ttgccgctca tgcaggagca gataatccct tcgccatccc
      721 attaagcgcc gttgtcggta ttccgctgta tatccgggct gaggcggtta tccctctggc
      781 atctgttctg atgacaaaag gaatgggtct gggagcatta atggcattaa tcatcggcag
      841 tgccggcgca agcctgacgg aagtgatatt acttaaatca atgttcagaa tgccgatgat
      901 agctgcattc ctgacggtta tattaggtat ggctatcttg atgggctatt tgactcaatt
      961 gctattttaa atagatgcca gcagtaaact ataagatgca tagttagtgc catatccttc
     1021 taagtagaaa atcatagaca ggataatcct taaaaatcac aaatgattta actaatgcat
     1081 tgagaatgat gacggctcgg tcaacaagtc atcaacagac aataaccacg taatcatgct
     1141 tctctgacat tttatcggag aagcaatgac tctttgtctc agtaccctcc tcctttttaa
     1201 aaggcagatt tcagtcgcta atacaacaca tttttaaata cactttaacc tggttcatta
     1261 ttacacatct aaaacatcac aaaaatatta cttcacacac tatcggagaa cataataact
     1321 ttttaaaatg agcgtttttt gatagttgtt aacgttttcc tttacgatac tatcactgcc
     1381 ctttttttat acacaaatat taccgggtgg aaatgaaagg gaatttaaag gtaattatat
     1441 caggaagata taacgcatta tatttattgt gtgtagaaga aaatccattc ctgttctaat
     1501 tatccaattt aaaaacatta gcataaaaca gatgatgaat aaggaatgtt atgtcaattg
     1561 actttacccc aggtataata aatacatatc acggcgatat ttataactgc acaacaaata
     1621 ccgataatgt taaaacgcca gacacaccga agtggccttg tgataattgg gaagaacaac
     1681 aacccattaa ttccacattc tctggagaag gatacaaccc tgaacaattt gatttagcgc
     1741 aacaacaact ccaacaaatt aatgcatgcc acacgaacac cacatataca aatgcagact
     1801 actcaaaagt tgtggttcaa cttgtaagtc ttattaataa cattgaaacg ataagttcga
     1861 caccacttac gcaacagaca caatcaatac ttaatcagat tcataacatt agatacgaaa
     1921 aggataagga ttctgtatgt caaatcattg ttgtcgttaa tccagaaccc aacaatccaa
     1981 tcataactaa aattctcgta gaagaaggaa tacccgaaag attcagtgtc caaaccgttt
     2041 cttccgataa taaaaatttt gctgggcaac gagctgactt accaactgat ataagagaca
     2101 ttcaaagctt gtacttaaaa atggcgaagc tgtatattga gcacagtaaa aatgaaaata
     2161 ggatggaagc ccttgctgga tgcgacttta ttgatttcaa catgacaggg caagatatgt
     2221 ccaagctagt cctaacctta tcaaaatttt attttgaaga tctacttaat ataaatttca
     2281 ctgacgcaaa tttatttaac actatttttt cacacaagga aaatcccatc cccaaactac
     2341 ataaatatga acaacatctt gacaaacaaa tcaatggttt gttttctaca ttgttaacta
     2401 ttaatgacaa ctctttacgc gcgaaagcgg aaattgcttc gagaataatt gatttcttag
     2461 aggcaaaggt agtgaatctt tcatttgatg acatattaaa atataagcaa gagttcaaaa
     2521 aaatatgtta taaacaactt caagagttta caacaccctc tctatacaat aaaattcaaa
     2581 aatgggcaac aatgtctaaa aatgaattca tagcatttca ttacgagaca cttcaacccg
     2641 aaaagatttc ataccccttt tatttaaaga gagatctgcc taatgaaaaa gatataaatt
     2701 atggggtgga aatagaaata ccatctggca aaagaattcg cttatcaaat cattatcaga
     2761 atataatacc ataatattga ttattagcac tttataatca ttgaataaaa atagatttta
     2821 tgtaatttta aaacaaaggg ttatattatt gggcgatgga tactcatacc acgccccaaa
     2881 ataacggaat tgaactaaac agaagggata taaacatcag acaggtttac gttactatca
     2941 ggcatatcac ctcagaatca gatgaaaact ataaagaaat atctattatg gttttaatat
     3001 ttgttgataa ggatagtaac atgaacatga caaaaggtgc actcatcctc agcctttcat
     3061 ttttgcttgc cgcatgtagt tcaattccgc aaaatatcaa aggcaataac caacctgata
     3121 ttcaaaaaag ttttgttgct gttcataacc agccggggtt atatgttggt caacaagcgc
     3181 gctttggtgg gaaggttatc aacgttatca atggcaaaac ggatacgttg ttagaaatcg
     3241 ctgtattacc gttggatagc tatgcgaagc ctgatattga agccaactat cagggccgac
     3301 tgctcgccag acaaagcggc ttccttgatc cagtgaacta tcgtaatcac tttgttacca
     3361 tcctcggtac cattcagggt gaacaaccag gctttatcaa taaagtcccg tataacttcc
     3421 tggaagtgaa tatgcagggc atccaggtgt ggcatttgag agaagtggtt aataccacct
     3481 ataacctgtg ggattacggc tatggtgcat tctggccgga accgggctgg ggtgcgcctt
     3541 actacaccaa tgcggtgagt caggtaacac ctgagctggt caaataacac caccgaaaga
     3601 tgcagatgct ttctcagcat ctgcatcatg tattacataa aattaatata cagtaagcta
     3661 actattatta ttataagccc tgtcctgtta attacctttg gcaaactgat tataaagtta
     3721 atgtccgcac caggagtcgg ttatgtttct tataattacc agggatacga tgttcttcac
     3781 cgcgatgaaa aacattctga gtaaaggtaa tgtcgttcat atacagaacg aagaagagat
     3841 cgacgtaatg ttgcatcaga atgccttcgt cattattgat acattaatga ataatgtatt
     3901 tcattctaat tttctcactc aaattgaacg attaaaacct gtccatgtca ttgttttctc
     3961 cccctttaat attaaacgct gtctggggaa agtaccggtg acctttgttc cgcggactat
     4021 cactatcatt gattttgtcg cactcatcaa tggcagttac tgctctgtgc ctgaagcgaa
     4081 tgtgtcactt tcgcgcaagc aacatcaggt tctgagctgc attgcgaatc aaatgacaac
     4141 tgaagatatt ctggagaaac tgaaaatatc gctaaaaacg ttctactgcc ataaacacaa
     4201 tatcatgatg atcctcaatc ttaagcggat caatgagctg gtacgccatc agcatattga
     4261 ttatctggtg tgaatttcag gcttacggtg agtctggcta cgctgccaca cagattagct
     4321 aattgaaacg cctttcaccc ctgccatacc ttttaataat cgcaacaggt cttctatcaa
     4381 cgttgtggca tgaagctgga tatcaatggc gaccacttca tgatcttcat tttcctgcaa
     4441 cgaaactaaa tcagttttta ttttttgctg cttaaaccag tcgagcattg ataccgtatt
     4501 accgttcact aatgtcagct gtaaatggta atttttgttc atcaggcgga aggttaattg
     4561 atggaagact tccagcacca acaaggtcat cactgaacca taaataccca gttcgtacat
     4621 accgctgcca ataaccatac ctatgcggcg gtcacccaga tatccgctgc cgtcgtcaga
     4681 cctacaatat tttggttacg aacgagaatg ttacctgcac cgataaaccc gacgcccgtc
     4741 accacctgag cagcaatacg gctggggtcg agtccgacgt gatctaaaga cagcacgtca
     4801 gcaaaaccat atttcgaaac aatcataaac agggcgcttc ccatgccaat taatacatgt
     4861 gtgcgta
//
LOCUS       DAJVRP010000186         4791 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVRP010000186 DAJVRP010000000
VERSION     DAJVRP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.188"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..157)
                     /locus_tag="OQN12_003935"
     CDS             complement(<1..157)
                     /locus_tag="OQN12_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1656083.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTY"
     gene            complement(307..468)
                     /locus_tag="OQN12_003936"
     CDS             complement(307..468)
                     /locus_tag="OQN12_003936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656084.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(465..653)
                     /locus_tag="OQN12_003937"
     CDS             complement(465..653)
                     /locus_tag="OQN12_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1656085.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            913..1248
                     /gene="iraM"
                     /locus_tag="OQN12_003938"
     CDS             913..1248
                     /gene="iraM"
                     /locus_tag="OQN12_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1656086.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1529..1637)
                     /locus_tag="OQN12_003939"
                     /pseudo
     CDS             complement(1529..1637)
                     /locus_tag="OQN12_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2160..2236)
                     /locus_tag="OQN12_003940"
     tRNA            complement(2160..2236)
                     /locus_tag="OQN12_003940"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Arg,seq:tct)
     gene            complement(2338..2413)
                     /locus_tag="OQN12_003941"
     tRNA            complement(2338..2413)
                     /locus_tag="OQN12_003941"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2378..2380),aa:Ile,seq:cat)
     gene            complement(2558..3379)
                     /locus_tag="OQN12_003942"
     CDS             complement(2558..3379)
                     /locus_tag="OQN12_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1656087.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3394..3750)
                     /locus_tag="OQN12_003943"
     CDS             complement(3394..3750)
                     /locus_tag="OQN12_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1656088.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3763..4740)
                     /locus_tag="OQN12_003944"
     CDS             complement(3763..4740)
                     /locus_tag="OQN12_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1656089.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVS
                     IAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTK
                     SHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1298 a         1307 c          983 g         1203 t
ORIGIN      
        1 gatatgtcag cagcccaaac agcagactcc ccagcacgcc tattgccgcc cactgagacg
       61 gggaaaccct gtccagcaac tgcaggaacc agtagcccgt tcccaccgct gacgtggtgt
      121 atgacacacc tgttgtgatt ttttccatct ggtccatacc ccgtctcccg ttatccggaa
      181 gctgacaaca ataaaaaagc caccagttaa ctactgatgg ctctgataac tcatgcaagc
      241 gtctcagacg acccactgac actaccggtg agtttaacga taccttccat ttgactggct
      301 cactttttat gatgatgccg gtgcatttat ctccagcacc agactttcta tctcaacgcc
      361 atacgttgca tttttggtaa tatccgtcag cgtcagtgca tttagtccca ctgccagact
      421 gtcttttatg gcctggaatg ccgggccagt acgatgacgt agtatcactc cggctcagtt
      481 gcaccactga ccaccacatc accttctgct gcaatcgcct gcatcagggt ataaggggtt
      541 atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt cagcttttcc
      601 gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc catggttctt
      661 tccaacatct gaaaaataat tctttaaaat acctgacatg taatacagaa aaaacacaaa
      721 accatacctt aaataaaaac ctgattatca agcagatatg catggataaa ctacaagacg
      781 agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa taattctgct
      841 ctatggttta attcaaaaat atcatttata cttttcagaa catcaccagc aaggcataaa
      901 caaggaaact aaatgaagtg gattgtgatt gatacagtta tccagccatc atgcggaata
      961 tctttttcag tcatatggag taaaataaaa ttaataatct ggtatcaatc ggatgctttc
     1021 ttacctcctg aaagtatatt tacactgact cacacaggca tcatgctcaa taacaaagtg
     1081 ctacctgtaa ccatttacaa cgtagtacca ttcaataaaa cattctggaa tttaatcaaa
     1141 aacagccagg aatgccctac aaatacagat aacgtattga atgaatgctt taataaccgt
     1201 tgcactctgc aaatatgtcc ttatggacta aaacaacaaa gtccataagg agtttactca
     1261 catctgacaa aatcaatata aacagcccct ccggagaggg gctggagagt ggcgctatgt
     1321 gccattccat ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat
     1381 tatcccatat acctactcgc tgattgcccc tccgcacagg gggattcacc atgccagttt
     1441 cttttaacaa actccccgca aaccagacaa cagtcaaccg cctgaattgt gaagtattta
     1501 aaaatttctc ccgctaactg atacccggct aacagtctgg cgttttcttt ttcagcaacg
     1561 ggaaagcaac aaccaccaca cccgccacca gcacaccgtc agccggcact gacattatcc
     1621 ggctgctgca atgccattaa caaaaacagt aagcaatcac tttttaccgt aacaggtgat
     1681 aatccagata tgtatctacc ccagatgagt aatccgaagt tcatccatac cacaggtcct
     1741 ggctattctg ttgtactcct gaacaagagc aaataattct gaattagcaa ccatgaactc
     1801 atcgcaaacc ctctgtatag catcactatt cagaataata acgtctcttc ccgaaagacg
     1861 atcaggagta cagaacaaaa ctgtcaaacg gctgaaggcc tttgctcgtg ctgcattgac
     1921 tatatcaata cgctgcctaa ggatgaaaca ccccgacgcc tcatcaatat tcactctacc
     1981 cacaccatat gaatgataaa tacttaatgc tgaaaaaacc attagaccgt ataacaaata
     2041 ctcaatcaat acttaacaga acttttattt ttgacaaata tataatattt tcaacaatat
     2101 cctgagccag gtatatttca gtataaggct ctgccggaag gaatctggaa gaatgaatat
     2161 ggcgcgctgt actggattcg aaccagtgac cgattgctta gaaggcaatt gctctgtccg
     2221 gctgagctaa caacgctgaa taccgataat ggaccgccat cggggacccg ccccccgcac
     2281 caacaaccct gttatcgtgt cgtctgctct tcctgataag ctaatggcgg tttgtgatgg
     2341 tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact
     2401 gagctaaagg gccgggagca gaataataac ggtccgtaat taatttcgca ataaaaaaac
     2461 ccgctcagcg gcgggttgta gaaactcttc taacgccagg cataaaacgc ccatcattat
     2521 gacgaattta ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact
     2581 gccgctcagc ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct
     2641 taactgtttt ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa
     2701 atgcctccgt tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga
     2761 taacaggcat accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc
     2821 aatccttaca ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta
     2881 ccacctccct gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact
     2941 ttgcccatac cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt
     3001 caataaattt ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat
     3061 aatcctcaaa agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa
     3121 gttttctcaa cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg
     3181 ttgcccgcgc cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca
     3241 tctcagcccg attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat
     3301 cttttgacgt tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg
     3361 gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac
     3421 ccccagacgt ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc
     3481 atcgtccatt aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc
     3541 caggtcgcga cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga
     3601 caggcttaat ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt
     3661 tccctcctcc gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg
     3721 cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt
     3781 tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc
     3841 gaacgccaga ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa
     3901 aatatcgtga gatttggtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc
     3961 gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc
     4021 gttaacccag cggcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac
     4081 ggcaatgctg accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag
     4141 cggcgcaaga ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccggtac
     4201 gatgtcgctt tcacggtaca ttgagcggat tttttccgca cgcaacccca gcgaacgacg
     4261 taataccgct tccggtagcg cgtccgccac ctgattgcgg accgcccacc aggataattc
     4321 agctagagat aattcacgct cctgcgtacc gcttattgcg tgaccgatga cgtcaatcat
     4381 ccatgctgac aggttttgat gagcaagttg ctcgagtgat tcggatgtct ggtcacgcag
     4441 ctggttgtcg cagtgccagc acaataccat tgcgccggta ccataacggt gaatgacggt
     4501 ttcgctgtga tgataatcgc cgtgtggcca ctggcaggat ttaatatggc gcaacagcca
     4561 gtcagacaat gcgccagcac caccagcagc acgaatcacc cgtgcgttac tgaaaaacgg
     4621 cagcaatgtt ttgtcttcca ctagcggctg gcgaacggca ggaacgaccc cggacggcag
     4681 attacgcatg cttttcggtt ccggctccac cagtacccgg gtattgtgga ataccggcat
     4741 ggattcacga ccgggcttaa ggaccaccag cccgagttcc ggaaccagaa c
//
LOCUS       DAJVRP010000187         4687 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVRP010000187 DAJVRP010000000
VERSION     DAJVRP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.189"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..142)
                     /locus_tag="OQN12_003945"
     CDS             complement(<1..142)
                     /locus_tag="OQN12_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HCS1656090.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLR"
     gene            complement(287..790)
                     /gene="cheW"
                     /locus_tag="OQN12_003946"
     CDS             complement(287..790)
                     /gene="cheW"
                     /locus_tag="OQN12_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCS1656091.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(811..2775)
                     /gene="cheA"
                     /locus_tag="OQN12_003947"
     CDS             complement(811..2775)
                     /gene="cheA"
                     /locus_tag="OQN12_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCS1656092.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(2780..3706)
                     /gene="motB"
                     /locus_tag="OQN12_003948"
     CDS             complement(2780..3706)
                     /gene="motB"
                     /locus_tag="OQN12_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCS1656093.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3703..4590)
                     /gene="motA"
                     /locus_tag="OQN12_003949"
     CDS             complement(3703..4590)
                     /gene="motA"
                     /locus_tag="OQN12_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCS1656094.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
BASE COUNT         1091 a         1297 c         1146 g         1153 t
ORIGIN      
        1 cccgtaattg attggaaacc acaaagctct tctggctatg gtgaagggaa gaaaaaaaca
       61 aactgccgga aataagctgt aacagtgcga atacccccag caccattacc aacagcgtga
      121 ctacgcggat acggttaatc ataaggcacc ttcctgaaaa caagttgatc tcgttatcgg
      181 caagaagggg ggaaacttta ttgctgatgc cacccgccgc gaaattgaaa taaaaaaccc
      241 gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg ggagaattac gccacttctg
      301 acgccgcgct atctaacagc gccatctctt cgctgttcag cagtttttcg atgttcacca
      361 gaatcaacat acggtcgccc agtgcgccca gtccagtgag atattctgtt gaaagcgtca
      421 cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa tgaaagcacg tctgaaacgc
      481 cgtcaaccac gatgccgacc acccgctgtc cgagattcag gacgataact accgtgttgt
      541 cgttatagtc cacatccacc tggctgaact taattcgtaa gtcaacaatc ggcacaataa
      601 cgccgcgcag attcgtgacg cctttgataa acgctggcgt gttcgcaatc cgtgttacct
      661 gatcgtagcc acggatctcc tgcactttca ggatatcaat accgtactct tcatcaccaa
      721 gggtaaatac cagaaattcc tggcctgacg gctcgctggc cagctttgtt acattcgtca
      781 taccggtcat attgttacct ttttactcat tcaggcggcg gtgttcgcca tacgttgttc
      841 gcggtttatc gcctgcaagg cggagacatc aacaatcagt gccacgctgc cgtcgccaag
      901 aatggtcgca gcagaaatgc cggggacttt gcgatagtta ctttcaaggt ttttaaccac
      961 aacctggtgt tgaccaatta attgatccac cagcaaggcg tagcggcgac cgccactttg
     1021 taagatcacc acaattccct gggtggcttc ggttttcgcg cccgcgacgt tgaacacttt
     1081 ccacagttcg acgatgggca gatattcacc ccgcacttcc agcacccgct cgccgccggc
     1141 cagtggatgg agatcggctt cacggggttg cagtgattcc ataacagcat tcagcggcag
     1201 aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg aggatggcca gcgtcagcgg
     1261 cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac tggatttcga catgaccgcc
     1321 catctcctgg atattacgtt taacgacgtc catgccgacg ccgcgcccgg agacgtcggt
     1381 gacctgctct gccgtggaga agcctggtgc aaatatcagc attgcaactt cgtcgtcgct
     1441 catgttttcg ctgacagtca aaccttgcga ggccgctttt gccagaattc gctcacggtt
     1501 tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg ccgccctgat gttcggcaga
     1561 cagaattaaa tttccgacgc tgtttttacc tgcggcgagc cgtttttctg gcagttcaat
     1621 accgtgatcg aggctattgc gtaccaggtg ggtcagcggg tcgataatgc gttctatcag
     1681 gctcttgtcg agctcggtgg aactgcccac cagcgtcagt tctacctgct tgccgagttt
     1741 tcccgccaga tcacgcacca gccggggata gcgactaaag acatattcca tcggcatcat
     1801 gcgaatcgac atcactgatt cctgcaaatc acgggcgtta cgttgtaact gccccatgct
     1861 ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg gaacgctggg caagcatgga
     1921 ctgggtgata accagctcgc cgacgaggtt aattaattga tcaacctttt ctaccgctac
     1981 acggatgctg gtggattcac tgctgcgcgt cgttttttcc cgctccacgc ggccggttgg
     2041 cgcttgttcg gctgccagtt taagcacagg tggggtagct atttttggcg aaacttctac
     2101 tgtttcaaag gtaatctgat cggcttcaat cacaaaacag agtaccgctg tgatgtcatc
     2161 ttcggcgata tcgcccggta atattgccga gagagaatcc gcccctttca ccacgtcatt
     2221 taacgttgtc agatgcccca gctcttcttc cagcaggtcg acttcgccgg ccttcaggcg
     2281 cgaaaggata attcgtcgcg gcaactgact gcgactttgc tcatcttgcg gttcactttt
     2341 ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg cctttcgctt ctaatgccag
     2401 ttgacgcaag gcctggcaga tataatcgta gctggcggca tccggctctt gcgactgttt
     2461 ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc aaaaacagat tgataatgtc
     2521 ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc aggttttcca tcagatgcgt
     2581 ggtttcctgc aaaacgctga agccaaaagt tcctgcccct cctttgatcg agtgggcagc
     2641 ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc ggctgcaaaa ccagcagatg
     2701 ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa aatgtctgat aaaaatcgct
     2761 tatatcgatg ctcacgctgt cacctcggtt cggctgatgg cattgtggga acactgacct
     2821 gtggtgcaac ctcaggtttt tctagggcgc ttactggctc attctggctt tcggcgtttt
     2881 catgcaaaat ggcctgttcg gcttgtttgt tcagtaccaa caggctgatg cgacggttga
     2941 cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc cgccatgccg acgacacgta
     3001 acactttgcc gctatccaac cctccgacca tcagttcgcg gcgggatgca ttggcccgat
     3061 cggcagaaag ctcccagttg ctgtaacctt tctcaccgct ggcgtaggga aaatcatcgg
     3121 tatgacctga aaggctaata cggttgggaa taccgttcag cacaggcgcg atggcgcgca
     3181 gaatgtcgcg catatagggt tcgacatcgg cactgccggt tctaaacatc gggcgattct
     3241 ggctatcgat gatctgaata cgtagacctt cctggaccag atcgattttg agatggagac
     3301 gtaacgcccg cagtttcgga tcggactcta tcaactggtc gagatcaccc cgcaatttcc
     3361 gcaatcgact ttgctccatg cgttttttca gctcttcgat gttcggctgc ttattcactt
     3421 ccccctggct ctgggtgtaa tcatcaccac cgccgggaat tgggctttca ctattggaaa
     3481 tgcgatcgcc gcccgtaacc gctgtcgcca gtggagtccg gaagtactcc gcaatctgaa
     3541 tcaactcttt tgggctggag atggagatca gccacatcac caaaaaaaag gccatcatcg
     3601 cagtcataaa gtcggcataa gcaatcttcc acgatccatg tgctgccccg tggcttttgg
     3661 ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt cttcatgctt cctcggttgt
     3721 cgtctgttgt tgcggatttt tcaccgcacg cacatgctct tccagttcaa taaacgacgg
     3781 acgttcgctg gaatagagcg ttttgcgacc aaactcaacg gcgataggcg gtgcgtaacc
     3841 gttcagatta gaaagcagag tgactttgac gcactgcatc attttgctgg tttcggcgct
     3901 tttctgacgt aaaacactcg ctagtgggga aataaatccg taagccaata aaataccgag
     3961 gaaagtcccc accatcgcat gtgcgataag cacccccagc tcggcggcag gacgatcggc
     4021 tgaacctaac gcgtgaacgc cccccattac agccgcgaca ataccaaacg ccggaagtga
     4081 gtccccgacc agcgccagac tgttcgccgg gacttctgcc tcgctttcgt gcgtctcaat
     4141 ctcttcatcc atcagggctt cgatttcgaa ggtgttcatg tgaccgctga taatcaggcg
     4201 cagataatcg acgataaaat caagcatgac gctatccgcg aggatgcgtg ggtagctggc
     4261 gaagatctcg ctctcacggg gattttcaat atcacgttcc agcgaaaaca tccccatctg
     4321 ccgcgatttc gccatcaacc gataaagcag agccagcaga tccatataca ttgctttggt
     4381 gtatttggag cgacgaaaca gcaacggcag cgctttcagc gtgcctttaa tcgctttgcc
     4441 attattgccg acgataaacg acccaatccc tgcaccggca ataatcacca gttcagcggg
     4501 ttgatagagt gctccaaggc ttccaccggt catcaaataa ccgccgaaaa ctgtaccgag
     4561 aacaaccagg taacctaata agataagcac gacatcatcc ttccactgtt gaccatgaca
     4621 ggatgttcag tcgtcaggcg ttaacgcgcg attggggaaa aaaaaagcag cggtacgtcg
     4681 ttaccgc
//
LOCUS       DAJVRP010000188         4648 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVRP010000188 DAJVRP010000000
VERSION     DAJVRP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.190"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(138..1079)
                     /locus_tag="OQN12_003950"
     CDS             complement(138..1079)
                     /locus_tag="OQN12_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCS1656095.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1091..1594)
                     /locus_tag="OQN12_003951"
     CDS             complement(1091..1594)
                     /locus_tag="OQN12_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656096.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(1599..2819)
                     /locus_tag="OQN12_003952"
     CDS             complement(1599..2819)
                     /locus_tag="OQN12_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS1656097.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLAS
                     ADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            complement(2834..3571)
                     /locus_tag="OQN12_003953"
     CDS             complement(2834..3571)
                     /locus_tag="OQN12_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCS1656098.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(3459..>4648)
                     /locus_tag="OQN12_003954"
     CDS             complement(3459..>4648)
                     /locus_tag="OQN12_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCS1656099.1"
                     /translation="QLDELQIWDALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETI
                     GEGKFKGILPLDRWMINPVLTRRIKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIR
                     FDGVTLPFQQKMTENEWGMSVVERIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRE
                     LIALGGPAFEALLKNIDLIRQFQSNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQI
                     SGAVGIPLVRLFGQSPKGFSTGDADLANYYDRVSSLQERRLRLPMRRILDIMHRSELG
                     KPLPDDFTFEFNPLWQMSDVDRSTVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVT
                     GIGASITDEDIENAEDEAPPGIGELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALR
                     WFK"
BASE COUNT          954 a         1333 c         1238 g         1123 t
ORIGIN      
        1 gcatgacggc ctttaacaaa ctcgcgccgc gaaccgtcgg gaagcgtgag cacaaacggg
       61 gtatgtacgt gtattactgc attatttttt gccatcgggt catccttaat ggccccgcca
      121 ggggggccat gtggctgtta aatgccatca acgtacgaaa tggtttcttt gtacactggc
      181 tcaaccgcac ccagcttgcc gtagtaggtc gcaatctgat acagaccgcg atactggata
      241 ggaacgctct gcaacggcac cagcggatag cggacgtatt ttttatcgtt ggtgtacgca
      301 accatgcgat ccttattccc cacaccacgg cctttcagcc atttaaccgc gcggatattc
      361 agcggaacac cgttctggtg atagctgatg gtgttggtct gaaggtacgt caacagggac
      421 tggttacccg cagatgaaac gatgatgctg gacaacagag caaactgctc tggcgggatc
      481 agcaaatcac gcggaaccac agagtaaccg gaagcggccc acgcatcaga cagcacctgg
      541 ttaatgcttg cgcggatttc gtccggtgtt gagattgccc acgttttggc agcgttgttg
      601 acaggcacgc cgtccagggt aacaaggcct ttcaggttta atgcggaatc gccaacatac
      661 acctgctcgt cgttgtccat ctgccatttg agctgcatcc cgtcgtactt ctgggtgtca
      721 atcgggcgac ctacctgctg agctgctgcc agctctacaa cggtccagcc cagttccatg
      781 ccccagagat tcagcgggtt accggatttg ccgatatcca cgtttacgcc agcaatagcg
      841 gttgagtctt tgcctaccca gtttttgcca ttcggatttg caccagtacc cgcagcggcg
      901 aagctggtat tcgtccagct ggaaatgtca tctgcgatag agacatcttc acgcaactga
      961 atatcgcggg tccaggtgta ccccgccagt ggcaggttca gagtctggtc gagtcgctcc
     1021 agctctccga tgagaaaggc accagagctg tcaacggttg cctgatcaaa agtaatcatt
     1081 cgtctgttcc ttaaatcttc caggaaattt ctgcattgcc gttagcatca ccggcacctg
     1141 tgaattcagc gttggtcagc accacatttt tgccactgac tgacgtggac atgaatccac
     1201 ccagcggcac tttgatggat tcatcagtgg agacgacaac gtataccggg tcgccttttt
     1261 tgatggtgct ggcatcaaaa tcagaaccga gattaacggt cacgtagcca cgcttcatgg
     1321 cgtcgcccgg gaagttcttg cctgttccca cctggcgaac catgtccggc tgcgaagtgg
     1381 tcggataagg gcgcacgtag atccccttca ccttgtctgc ggtatcgcca tctgccagcg
     1441 gcacgaaaaa accgtcagca tcgtatttac cagccagccc ataggcagca aaggcgttat
     1501 cggatttaag gaccaccggt tcgacggtta agtcctgcgg gcgagagata gccccggcaa
     1561 tgccaacagg catccggtac agatatgcag tcattggatt atcctttgcg gttagaccag
     1621 aattcagcgt tttgtttgtt cagggaagcg atgctggtca tgcccatgtt tggacgttgt
     1681 gcatcgcccg tggtgctgcg ggtgtttcgc cctttggcaa tctcagacac agcgttaaac
     1741 gccatatcga ccgattgctt aggcaatttg cggatatccg catcaccgac aacctggcga
     1801 accagcgttt tgtcagcaga agccagaacc tcgcgtttga acacggtcgg tttcatctta
     1861 cggctcagat cgatacccgg aacgataact tcggcacgcc aggctgagtc accagtaatc
     1921 gtggtttcct cttcatcgtc ctcgccgtca ccggtcggat tatcgtcagg tttattatcg
     1981 ttatcgcccg tggcatttcc ttccagctta gccagcaggg ctttcagtaa tgttttgagg
     2041 tcatcatcac tgtcgccggt tggacctccg cccatctctg gtactttgtc cggtagcggt
     2101 tgctgcgggg acaggttgat gttgagatta acgccctgcg gcaaatcccc ctcatctcct
     2161 gtaaccgatg cgggagccga ctccaccagt tcgttcatgg tgtcagcgtc acccgttttg
     2221 atggccgtgc gcatgcgggt ccaccagctt ttcttttgat ttgccattgt gtctctgtct
     2281 ccaattgcac aacgatttcc ggctctgcct ttggggacaa gagccacatg gtttccggta
     2341 atatcgacct gctcggcttt acctggctcg gtctgctcgt actccgcgtc atagccgcac
     2401 gacacttcac gcaggccatc ttcgataagc tgaatggcgt tttcgtcttt gacgataagg
     2461 tcagccagca tcaaatcaga gtggtcaccc gtcccgcgcc gcacattctg aagatgcccg
     2521 acagcaagct ctttccagtt ctcgggattt accagccgca cattcccgtt ttcatcttca
     2581 ggatgcagga tcgtgatgct catcccttcg aatgaggcga gcgtggccgg atggaatacc
     2641 tgctcaggag agcgcgttac gactatctca ccgagcttgt cgggtttgag gtttggcaga
     2701 tcggcagcgc cgtagagctg tttacccgtt cgacctatcg gcacgtcttt gcacagcagc
     2761 gagccgtcag ccagctgata gcgggtttcc cccagccggg tattgaaaaa atatttcatg
     2821 gtttacctgc gattcaggcg agataagaat gagggttggg aaaaacgatt tctttataac
     2881 agcgacaatt cgggagctcg ccagcgtgac cggtcatgcc atcaagcgtt ggaggtttgc
     2941 cccattcgac aaacttacct tccatctccc gatgagaatg ccggacgtcg ccatcttcgg
     3001 ctgtacgcca gatataacca ttcgagccga ttgacagcgc acgcgcctga tcgagcgcgc
     3061 cggttgcacg tccaagctcg gtacgggcga taaggttcgc tcgtgagcgt gacacgtcac
     3121 cggacgctgc tatctctttc gcgaatggtt cagcgcggcc accagccaca acggcctcga
     3181 tggccttgtt ctgaatgtca tacacccgat cggcggcctc aagaggcagt gacttgatat
     3241 acttaatttg ctcggcgacg atggatttca tcacctggcc taccggggcg cggtcgacca
     3301 tgttgcgcag ctctgcgctg atgttccggc tgtgctgacg ccactgcttt tcattctggc
     3361 gtgcaatgtc ggcggtaaag ttctcagcaa ccttcgtcgc ccagggggtg atgatttcgc
     3421 tgtagcgctc cagcgcatcc attatttcgg taacgctatc atttgaacca tcgtagcgcc
     3481 catttacgat atccccgacc gcccgcgcta tctgccgtag gctcgttcga tatcggatct
     3541 ccgcctggcg gctctggcgg tttgtcgcca agttcgccga tgcctggcgg cgcttcgtct
     3601 tcggcattct cgatatcctc gtcggtaatg gatgccccga tgccggtgac gtcagagttt
     3661 tcgcgcaggt cggtcatcgc cgccttacgc gtcatcaatc cgtcgcccag cgcggtacta
     3721 atcgcgttgg tggtgtttac ggccaccgtt gatcggtcaa cgtctgacat ttgccatagc
     3781 gggttaaact caaacgtgaa atcgtccggc agcggctttc cgagttccga gcggtgcata
     3841 atgtccagta tccggcgcat cggcagccgt aagcggcgct cctgcaatga gctcacccgg
     3901 tcgtaatagt tggcgaggtc tgcatcacca gtagagaagc ctttcgggga ttgaccgaac
     3961 aaccgcacca gcgggatacc aacggcaccg ctaatctgct cagcaaactg cgaaagaatg
     4021 tcatccagac cactaaagct gtactggtgg gtttcgaact tatcccgcga gtccatgagc
     4081 gtcatacctt cattgctctg gaactggcgg atcaggtcga tgttcttcag caacgcctcg
     4141 aacgccgggc ctccaagcgc gataagctcg cgcaacttct ccacgctata ggtacgcaga
     4201 tgtgctttgt agaccagctg cgccgcgccg acagtggcgc tgtcgaacgc agtaagccga
     4261 tcccagatac gctctacaac cgacattccc cattcgtttt cggtcatctt ctgctggaat
     4321 ggcagcgtca ccccgtcgaa gcgaatcagg cggctgtgat ggatgcgcca ggccgggatg
     4381 cccgttgcag tggtcaccac gtcgtaaaac tcaggtttgc cgagatctgg ccccatctct
     4441 ttaatgcggc gggtcaggac cgggttaatc atccagcggt cgagcgggag aatgccctta
     4501 aacttgcctt ctccaatggt ttcgagccgc agcggggtca ttggtgcctg cccctcgatc
     4561 atgatgaagc cgaccgcgcc gccgtagagg cgagaccatt tcagcacgtc gttcagcgca
     4621 tcccagattt gcaactcatc cagttgtg
//
LOCUS       DAJVRP010000189         4642 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVRP010000189 DAJVRP010000000
VERSION     DAJVRP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.191"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..312
                     /locus_tag="OQN12_003955"
     CDS             <1..312
                     /locus_tag="OQN12_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1656100.1"
                     /translation="ALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYC
                     QYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASM
                     A"
     gene            469..1218
                     /locus_tag="OQN12_003956"
     CDS             469..1218
                     /locus_tag="OQN12_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1656101.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1240..1806
                     /gene="yieF"
                     /locus_tag="OQN12_003957"
                     /gene_synonym="chrR"
     CDS             1240..1806
                     /gene="yieF"
                     /locus_tag="OQN12_003957"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCS1656102.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="OQN12_003958"
     CDS             complement(1860..3197)
                     /gene="adeP"
                     /locus_tag="OQN12_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1656103.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3362..4018
                     /gene="yieH"
                     /locus_tag="OQN12_003959"
     CDS             3362..4018
                     /gene="yieH"
                     /locus_tag="OQN12_003959"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS1656104.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4085..4558
                     /locus_tag="OQN12_003960"
     CDS             4085..4558
                     /locus_tag="OQN12_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCS1656105.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1241 a         1163 c         1139 g         1099 t
ORIGIN      
        1 gcgctggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat gagcctgccg
       61 gaattcgagc agtcactgtt tatggcagcg caacccgaca atctgctact ggcgaccgcg
      121 ccgcgctact gtcagtacta caatcaactc catcaactgc cgttggttgc tcttcctctc
      181 ccgtttgacg aaagccagca aaaaaagctg gaagttcctt ttaccctgct gtggcataaa
      241 cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg
      301 tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag
      361 ttgtaataag cttgtctgaa tcgaactttt agccgcttta gtctgtccat cattccagta
      421 aatgattact cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac
      481 tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt
      541 catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt
      601 ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc
      661 ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc cttcgttttc tatttcctat
      721 gccgggaata tggttggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa
      781 ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca caccttttcc
      841 agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc
      901 attacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc
      961 gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa
     1021 gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa
     1081 aagctcagtg tctgggagtt agatggcaaa cacagctgga aaagcctgcc ggatattcac
     1141 agccgcgcca acaatcctac cagccggatg atgcgttttg cccagctctc taccgtgaag
     1201 tctttttcgc caaattgata gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg
     1261 gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg
     1321 ccgaaaattg ctccggcgag catggaagtc aatgcgttac cttccattgc cgacattccc
     1381 ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag caacggttga agctctggcg
     1441 gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctactcggta
     1501 ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggcc
     1561 ggtaaaccgg tgttgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag
     1621 tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccggaattt
     1681 atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gagaagtgat tgatcagggt
     1741 acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag
     1801 atctaaataa aaaacccgcc agtaatcatg catggcgggt ttttaatgcg ctatcgattt
     1861 taatgagcgt cgataaatac aatcttcagg ataaacagca gcgcaacgat gattacgcac
     1921 gggctaaggt cacgcagacg cccggtacca atcttcatca cgcagtagga gataaagccc
     1981 agcgcaatac cttcggtaat cgaaaagctg aacggcatca tcacggcggt aataaacgcc
     2041 ggaacagatt cagtaagatc ctgccagttc acgcgagcaa gacttgaggt catcaacacg
     2101 ccaacgtaga tcaacgcgcc agctgcagcg tagcctggca ccattcccgc cagcggcgac
     2161 agaaagataa ccagcaggaa cagcagacca acaaccactg ccgtcagacc ggtacgaccg
     2221 ccaaccgata cgccggaaga ggactcaata taagccgtaa cggaagaagt accgataaac
     2281 gaaccggtca cggaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaatttc
     2341 cccttctcat ccgccagacc tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca
     2401 aacaagttga ccaacatgaa agagaaaatc acccctgcca gcccgaggtt aaacgacccg
     2461 gctaaatcta catgacccac aactgtcatt acgctcggcg gcgcagaaac gatgccattg
     2521 tagtgcacat cacccagcat ccagcccagc agcgtcgtca ccacgataga aaccagcacc
     2581 gctgcgtgaa tgttgcgcga ggccagaata gcaatgatga aaaagccgag gatacccaga
     2641 agtacgctgt gagaagtcag attaccgatg ctcatcagcg tttccgagtt agcgacaatc
     2701 acacctgcgt ttttcagccc catcatgccg ataaacaggc cgataccgct ggtaataccc
     2761 acacgcagac ttaccggaat gttagcaatc atccagtaac gaacgcggaa aatggtcaaa
     2821 agaagcagtc cgacagcgcc ccagaagatt gcgcccatac cgatctgcca cggcagcccc
     2881 attgcctgga caaccacaaa agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc
     2941 accggaaggt ttgcaaacaa tcccatcata atgctaccga aagcagcaat cagacaggtt
     3001 gttacgaaga cggcactggt gtccatgcca gcaacgccca gaatttgtgg gttaacaaat
     3061 acgatgtaga ccatcgtcag gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta
     3121 gtaccatgtt cgcgcagttt aaaaacacgt tccagcatcc tctgaccaga agtctgggtg
     3181 gtatgttgat gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat
     3241 accaaaatgc gacaataggc gcgtttgtga gagacttttt tattggattt acttatacgg
     3301 caacgattgc gttgcgcaaa tcggctctac gaaaacgtta aactgattaa aaaggaaagg
     3361 catgtcccgg atagaagcgg tatttttcga ctgcgacggt acgctggtcg acagtgaagt
     3421 catttgctcc cgcgcatatg taacgatgtt tcaggaattt ggtattacgc tcgatcctga
     3481 agaggtattc aaacgtttca aaggtgtaaa actgtacgaa attatcgata ttgtttccct
     3541 tgaacatggt gttacgttag cgaaaacaga agctgaacac gtttaccgtg cagaagtcgc
     3601 tcggctgttc gattcagcca tcgaaggggc tggagcgctc ctgtcagcga tcactgcgcc
     3661 aatgtgtgtg gtatctaacg gcccaaataa caaaatgcag cattctatgg gcaagctgaa
     3721 tatgttgcac tacttcccgg ataaactgtt cagcggctac gatattcagc gctggaagcc
     3781 agacccggcg ttaatgttcc atgcggcaaa agcgatgaat gtaaatgtag aaaactgcat
     3841 tctggttgat gactcagttg ccggtgcgca atctggtatc gacgcaggta tggaagtgtt
     3901 ctacttctgc gccgacccgc acaataagcc gatcgttcac ccgaaagtca ccacctttac
     3961 ccatctttcg cagttaccgg aactgtggaa agcgcgtggt tgggatatta cggcatagtt
     4021 cttcacactc ccttcactta ccccgcttaa attggcgctc aaaggtaagt aaagggagtt
     4081 tgatatgtct gtttcacgtc gggtaataca tcacggactt tattttgcgg ttttaggacc
     4141 gttaattggt gttctgtttc ttgtcctcta catattcttc gcaaaagaac cgctggttct
     4201 tttggtaata atacaggtac ttccattgtt tctcttattg tcgattacta cgggagctat
     4261 tcctgcattg ttaaccggcg tgatggtcgc ctgtctgccg gaaaagatcg ggtcacagaa
     4321 aaattatcgt tgtctggctg gtggcatagg tggcgtcgtt atcaccgaga tctattgtgc
     4381 agttattgta catattaagg gcatggcttc ctcggagttg tttgaaaaca ttctttctgg
     4441 tgacagtctc gttgtccgca tcattcctgc attgctggca ggtgtggtga tgagcagaat
     4501 cattacccgt ctgcccggat tggatatttc atgtcctgaa acagactctt taagttaagc
     4561 gggatacttt atctttgggc tactcaaaag cagacaggat gttactatga ctcaaaatat
     4621 caggccgtta ccccaattca aa
//
LOCUS       DAJVRP010000190         4611 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVRP010000190 DAJVRP010000000
VERSION     DAJVRP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.192"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            224..2647
                     /gene="pgaA"
                     /locus_tag="OQN12_003961"
                     /pseudo
     CDS             224..2647
                     /gene="pgaA"
                     /locus_tag="OQN12_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2656..4149
                     /gene="pgaB"
                     /locus_tag="OQN12_003962"
     CDS             2656..4149
                     /gene="pgaB"
                     /locus_tag="OQN12_003962"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCS1656106.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            4153..4611
                     /gene="pgaB"
                     /locus_tag="OQN12_003963"
                     /pseudo
     CDS             4153..4611
                     /gene="pgaB"
                     /locus_tag="OQN12_003963"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT         1317 a         1034 c         1061 g         1199 t
ORIGIN      
        1 tttatgccgt attcctgaag atcctcatca ttggaatgga ttttcgggcg agaaaaggat
       61 tttatatgga cactctgctc atcatttctt cttctcatca tcaacaattc acgtctctct
      121 tccgcgttta ataacggatt atgaggtgca aaaatatctt tcttttcagt tacctgtaat
      181 tagatacaga gagagatttt ggcaatacat ggagtaatac aggatgtatt caagtagcag
      241 aaaaaggtgc ccgaaaacca aatgggcttt gaaacttctt actgccgcat ttttagcagc
      301 gagtcccgcg gcgaagagtg ctgttaataa cgcctatgat gcattgatta ttgaagctcg
      361 caagggtaat actcagccag ctttgtcatg gtttgcacta aaatcagcac tcagcaataa
      421 ccaaattgct gactggttac agattgcctt atgggccggg caagataaac aggttattac
      481 cgtttacaac cgctaccgtc atcagcaatt accagcgcgt ggttatgcag ctgtcgccgt
      541 cgcttatcgt aacctgcaac aatggcaaaa ctcgcttaca ctgtggcaaa aggcgctctc
      601 tctggagccg caaaataagg attatcaacg gggacaaatt ttaaccctgg cagatgcggg
      661 tcactatgat actgcgctgg ttaaacttaa gcagcttaac tctggagcac cggacaaagc
      721 caatttactc acagaagcct atatctataa actggcgggg cgtcatcagg atgaattacg
      781 ggcgatgaca gagtcattac ctgaaaatgc atctacgcaa caatatccca cagaatacgt
      841 gcaggcatta cgtaataatc aacttgctgc cgcgattgac gatgccaatt taacgccaga
      901 tattcgcgct gatattcatg ccgaactggt cagactgtcg tttatgccta cgcgcagtga
      961 aagtgaacgt tatgccattg ccaatcgcgc cctcgcccaa tacgctgcat tagaaattct
     1021 gtggcacgat aacccagacc gcactgccca gtaccagcgt attcaggttg atcatcttgg
     1081 cgcgttatta actcgcgatc gttataaaga cgtcatttct cactatcagc gattaaaaaa
     1141 gacggggcaa attattccgc cctaggggca atattgggtt gcatcggctt atctcaaaga
     1201 tcatcagccg aaaaaagcac agtcaataat gaccgagctc ttttatcaca aggagaccat
     1261 tgccccggat ttatccgatg aagaacttgc ggatctcttt tacagccacc tagagagtga
     1321 aaattatccg ggcgcgctaa ctgtcaccca acataccatt aatacttcgc cgcctttcct
     1381 tcggttaatg ggcacgccta cgagcatccc gaatgatacc tagttacagg ggcattcgtt
     1441 tctctcaacc gtagcaaaat atagtaatga tcttcctcag gctgaaatga cagccagaga
     1501 gcttgcttat aacgcaccag gaaatcaggg actgcgcatt gattacgcga gtgtgttaca
     1561 agcccgcggt tggcctcgtg cagcagaaaa tgaattaaaa aaagcagaag tgatcgagcc
     1621 acgtaatatt aatctggagg ttgaacaagc ctagacagca ttaacgttac aagaatggca
     1681 gcaggcagct gtcttaacgc acgatgttgt cgaacgtgaa ccgcaagatc ccggcgttgt
     1741 acgtttaaaa cgtgcggttg atgtacataa tcttgcagag cttcgtatag ctggttcaac
     1801 aggaattgat gccgaaggcc cggatagtgg taaacatgat gtcgacttaa ccaccatcgt
     1861 ttattcacca ctgctgaagg ataactggcg cggttttgct ggattcggtt atgccgatgg
     1921 acaatttagc gaaggaaaag ggattgttcg cgactggctg gcgggtgttg agtggcggtc
     1981 acgtaatatc tggctcgagg cggagtacgc tgaacacttt ttcaatcatg agcataaacc
     2041 cggcgcgcgc ctgtctggct ggtatgattt taatgataac tggcgtattg gttcgcaact
     2101 ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa aatggtgtta caggcaacag
     2161 tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt aagtacggtg tctcctgggc
     2221 tttcactgat ttttccgaca gtaaccagcg tcatgaagtc tcaattgagg gtcaggaacg
     2281 catctggtct tcaccatatt tgattgtcga tttcctaccc agtctgtatt acgaacaaaa
     2341 tacagaacac gataccccat actacaaccc tataaaaacg ttcgatattg ttccggcatt
     2401 tgaggcaagt catttgttat ggcgaagcta tgaaaatagc tgggagcaaa tcttcagcgc
     2461 aggtgttggt gcctcctggc aaaaacatta tggcacggat gtcgtcaccc aactcggcta
     2521 cgggcaacgc attagctgga atgacgtgat tgatgctggc gcaacgctac gctgggaaaa
     2581 acgaccttat gacggtgaca gagaacacaa cttatacgtt gaattcgata tgacattcag
     2641 attttaagga taaatatgtt acgtaatgga aataaatatc tcctgatgct ggtgagtata
     2701 attatgctca ccgcgtgcat cagccagtca agaacatcat ttataccgcc acaggatcgc
     2761 gaatctttac tcgccgagca accgtggccg cataatggtt ttgtggcgat ttcatggcat
     2821 aacgttgaag acgaagctgc cgaccagcgt tttatgtcag tgcggacatc agcactgcgt
     2881 gaacaatttg cctggctgcg cgagaacggt tatcaaccgg tcagtattgc tcaaattcgt
     2941 gaagcacatc gaggaggaaa accgctaccg gaaaaagcag tagtgctgac ttttgatgac
     3001 ggctaccaga gtttttatac ccgcgtcttc ccaattcttc aggccttcca gtggcctgct
     3061 gtatgggccc ccgtcggcag ttgggtcgat acgccagtgg atgaacaagt aaaatttggc
     3121 gatgagatgg tcgatcgaga atattttgcc acgtggcaac aagtgcgaga agttgcgcgt
     3181 tcccggctcg ttgaggtcgc ttctcataca tggaattctc actacggtat tcaggctaat
     3241 tccaccggca gcttaatgcc tgtatatgta aatcgtgcat attttactga ccacgcacgg
     3301 tatgaaaccg cagcagaata ccgggaaaga attcgtctgg atgctgtaaa aatgacggaa
     3361 tacctgcgta caaaggctga ggtaaatcca cacgtttttg tttggcctta tggcgaagcg
     3421 aatggcatag cgatagagga attaaaaaaa ctcggttatg acatgttttt cacccttgaa
     3481 tcaggtttgg caaatgcgtc acaattggat tccattccgc gagtattaat cgccaataat
     3541 ccctcattaa aagagtttgc ccagaaaatt attaccgtac aggaaaaatc accacaacgg
     3601 ataatgcata tcgatcttga ttacgtttat gacgaaaacc gccagcaaat ggatcgcaat
     3661 attgatgtgc taattcagcg ggtgaaagat atgcaaatat caaccgtgta tttgcaggca
     3721 tttgctgatc ccgatggtga tgggctggtc aaagaggtct ggtttccaaa tcatttgcta
     3781 ccaatgaaag cagatatttt tagtcgggtt gcctggcaat tacgtacccg ctcaggtgta
     3841 aacatctatg cgtggatgcc ggtattaagc tgggatttag atcccacatt aacgcgagta
     3901 aaatacttac caacagggga gaaaaaagca caaattcatc ctgaacaata tcaccgtctc
     3961 tctcctttcg atgacagagt cagagcacaa gttggcatgt tatatgaaga tcttgccgga
     4021 catgctgctt ttgatggcat attgttccac gatgatgctt tgctttcaga ttatgaagat
     4081 gccagtgcac cggctatcac ggcttatcag caagcaggct ttagcgggag tctgagcgaa
     4141 attcgataaa acccggagca atttaaacag tgggcccgct ttaaaagtcg tgcgttaact
     4201 gacttcactt tagaacttag tgcgcgcgta aaagccattc gcggtccaca tattaaaact
     4261 gcacgaaata tttttgcact tccggtaata caacctgaaa gtgaagcctg gtttgcacag
     4321 aattatgctg atttcctaaa aagctatgac tggaccgcta ttatggctat gccttatatg
     4381 gaaggtgtcg cagaaaaatc ggctgaccaa tggttaatac aattgaccaa tcaaattaaa
     4441 aacatccctc aggctaaaga caaatctatt ttagaattac aggcacaaaa ctggcagaaa
     4501 aatggtcagc atcaggctat tccttcgcaa caactcgctc actggatgag cctattacaa
     4561 ctgaatggag tgaaaaacta tggttattat cccgacaatt ttctgcataa c
//
LOCUS       DAJVRP010000191         4564 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVRP010000191 DAJVRP010000000
VERSION     DAJVRP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.193"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="OQN12_003964"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="OQN12_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1656107.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="OQN12_003965"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="OQN12_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1656108.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="OQN12_003966"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="OQN12_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1656109.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="OQN12_003967"
     CDS             complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="OQN12_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1656110.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4189..>4564)
                     /locus_tag="OQN12_003968"
     CDS             complement(4189..>4564)
                     /locus_tag="OQN12_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1656111.1"
                     /translation="YKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYY
                     SVSSLGTRWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYE
                     IYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1230 a         1103 c         1090 g         1141 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atatcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa cgcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaacgctgta taaggggcaa
     3961 aaaaggggca aaaaaggggc aaaaaagagt gggttatcgt agcttattgt tgtcgctgat
     4021 aatgtttaac atattgaaaa ataaataaaa tacttatgag tcagatagtt gtgatttttg
     4081 cccttacttg ttcaggtcgt attgttcttt ctttgtaatt tgttgatttt cttgcattat
     4141 ttcagttctc tggtactaaa tggggcaaat tgggggcaaa ctttgcaact acgataaccg
     4201 cgcattcaac atggctatct gttcgtcgtt catgtcatca atccacatac cgtaaatttc
     4261 atacaccatt tgcgcagttt catgccccat ctggctggct ataaatgccg ggttcgctcc
     4321 tgccgtcaac agccagcagg caaaagtatg tcgcgtgtgg tacggattac ggcggcgaat
     4381 accagcacgt tttactgctg cattccatct tgtacccaaa ctgcttaccg agtaataagg
     4441 tttctgtttt tcgttacaca ccctgggcat gaaaacaaaa tgcagttttt gtttttcagt
     4501 tctgccgtac tcccgatgat aaaaagtgat ttcgcttttg cgatgatgcc cggtcagttt
     4561 gtat
//
LOCUS       DAJVRP010000192         4562 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVRP010000192 DAJVRP010000000
VERSION     DAJVRP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.194"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(247..1149)
                     /locus_tag="OQN12_003969"
                     /pseudo
     CDS             complement(247..1149)
                     /locus_tag="OQN12_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1209..1487
                     /locus_tag="OQN12_003970"
     CDS             1209..1487
                     /locus_tag="OQN12_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656112.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1488..2015)
                     /gene="gadE"
                     /locus_tag="OQN12_003971"
     CDS             complement(1488..2015)
                     /gene="gadE"
                     /locus_tag="OQN12_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1656113.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2814..3386)
                     /gene="hdeD"
                     /locus_tag="OQN12_003972"
     CDS             complement(2814..3386)
                     /gene="hdeD"
                     /locus_tag="OQN12_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1656114.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3641..3973
                     /gene="hdeA"
                     /locus_tag="OQN12_003973"
     CDS             3641..3973
                     /gene="hdeA"
                     /locus_tag="OQN12_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1656115.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            4089..4415
                     /gene="hdeB"
                     /locus_tag="OQN12_003974"
     CDS             4089..4415
                     /gene="hdeB"
                     /locus_tag="OQN12_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1656116.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNENDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4479..>4562
                     /locus_tag="OQN12_003975"
     CDS             4479..>4562
                     /locus_tag="OQN12_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCS1656117.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGK"
BASE COUNT         1343 a          971 c          958 g         1290 t
ORIGIN      
        1 cattgacctg cctttagggt gcctttaaaa ttcgttttta aaggctacga tgacattttt
       61 gaagagtctt aaaagaatta aaatacacgg cgcgcctatc ttgaatgttt tatcgatatc
      121 gaagcattga gcatttttaa ctcgttttat cttgaatgca atcggcgcat aacttattga
      181 atcccatcaa atcccgtctt tttttaccca ttcccggtta tcttgaataa attacctagt
      241 cataaataca ttctggcggc taccttcatc cactaatgcc gtgacataca tgccaggcag
      301 caagtcacca tttgggttgg ggaaaatcgc ccgtaacgtc acggagcccg tggtttcatc
      361 aaccgtcggg tcggagaatt tcagcgtgcc ggtctggctg taacgtttac cattttccag
      421 attgagctgt actggcgtac tgccctgaac ctgtttgatt tgcccactgg cgacctcttc
      481 tttcatgcgt aagaaatctt gcaccgactg cgtgagatcg acataaatcg ggtccagacg
      541 ttgtacggta accagcgaat ctgcctgatt agcggtaacg agtgcgccga cggtcaccga
      601 cgatttcccg ctgacgcccg taatcggcga ggtgacattc gcgtattgca gattgatcgt
      661 cgcctgttca acagccgctt tggcgacggt gacattggct tctgcttcat tcagctgggt
      721 gcgcgcggtg tcgtaatcct gacggctaac gtagttggtc ttcagcaacg atgcctggcg
      781 gttaaaggtg atgcgggcat tgctggcggt agagagcgct ttcgccagcg agcctttggc
      841 ggagtttagc tcggcctgta aaggtgcagg atcaatctga tacagcgaat cgccctggtt
      901 cactttatcg ccttcgataa agttgcgttt aatgataata ccgcccacct ggggacgtat
      961 ctcggcaact tcataaggaa cggttctacc gggcaattcg ctcaacacat tgaccgaacc
     1021 aggggagagt gtgacgacac cgacctcagg cgtcatggcg gcggcgtttt ccgccgattt
     1081 gtcatcgcag gcggtgagca tcgcgccgca gaataacaac ggtattaaca gctttcttct
     1141 tctgttcatt ttagtccctg aaaattcttg agaaataacg gttctttaaa ttgcgcgcga
     1201 ggagagatat gagggattta cagaccagcg gcatagtggg gttgtcagcc agtaaggtgg
     1261 gatacaggca cagtgatcga catggtgagg tcaacgacaa aaaaaatgtt gccgttctgc
     1321 caacagttcc tgccagcatt cgggcaacga agggttctac tggtggatac acataccagg
     1381 ggaatcaagt cgttttagcc tctcctttag taatcaccgg gggtaatgaa atcagcatct
     1441 gcatccctcg tcatgccagc catcaatttc agttgcttat gtcctgacta aaaataagat
     1501 gtgataccca gggtgacgat gtcgctcata cgtcttagtt tcagcttgta cataatgttg
     1561 aatttctgaa tctttaatgt ttgttgcgaa atattgagcg tcgacgtgat attgctttca
     1621 tttttatttt tcagaatcag ttgcataatt ttgaactgat tatgtgaaaa acataattgt
     1681 gaattcttat ggggcaagtg tttaccataa gtaatctcaa gaagattttt gatactttct
     1741 ttgcggctaa caaaataaat aggcgcttta gcttttagtt tactgatgtg ataaggtgcc
     1801 aaaacaataa ttctctcggc atctaatttc tccagaaatt taatcgcttc ttcatcaagg
     1861 atatgattgt gataggtatc aatgataacc ttgaagggtt tattgcctac ttttttgatc
     1921 tctgacaggg aattgatttt tatcatttcg tgattatctt tcaactgcca aaagccctgt
     1981 aaaagaaaag aatctttcgt catgagaaaa atcataactt gctccttagc cgttatcgtt
     2041 tcgaacatgt catccttttc attatttaca tccttgtccg aatcgttgtt caatatagta
     2101 aacgcctata agaactttgc ccttcgtgaa ctaatacgtc tattaacact aatcaatact
     2161 tcagatttat ccatcacgca ctccatatat tgatcctaag taagattcct ggttgttatc
     2221 agcttgtaaa tagtaaacaa aaagttaacg tttgcggatt actgcccaag aataagttat
     2281 caataaccag tttgtgatct ctgaagaata ttactaaagt taaaatgtaa tccgatttaa
     2341 atatcgagtc tccttgtttc gacttaagct ggcaattgga ttgccagctt tcttttaacg
     2401 cattgaaatt acctgagtaa ataacgcatt catgtcttca ggtttttata tagcgtctgg
     2461 agacacggat atttatgcaa tgtaatcaac gccagttgca aaaaaatgaa cccggattac
     2521 ttatttaacc gcccatagca tcaacgctta cggaactctt tgttcacttt cccacagcac
     2581 ttttaaaaca ttaaacctga ctacggaaaa tatcagccat gataatgttt gaaatggcta
     2641 atttgccatg gagtgaaaaa aattagatga aattcagtag gttgaaataa tcactagcag
     2701 gtaattattt caatgatagt gcgcaattga tctacaacac tgcgtagcgg agagagtatt
     2761 aatcggatca tagtcacatc aagtgactat gatccgggtg acaaccgggg taattattgc
     2821 tgcttaacga acaaactggc gaagctgaac aggctggcgg cgctaaatat cagttcaatt
     2881 cccaccagtg tggaaaccag cgttacagac accatcggcg ttgcaccaag gaatatccag
     2941 gcaatgacga tatccagcac accaataacg agctgtagcc agctgccttt cattgaacgc
     3001 tgacgatacc aactcatcag gcgaataacc cctgcaacac agaacaaacc ggcaataaat
     3061 gccgcaatgg caaaaatgcc cagctccggt gcacggatga agaaatagcc gatcaataaa
     3121 taggcgactg cgacgaggaa accggataat accggccaga aattatgact gcggttgctg
     3181 aataacccga caataagcgc aatacccgag cagattaata atgcacccac tactgtgctt
     3241 aaaatatcgc cagagacgaa cgggaaactg atacacagca acccgacgat aaacagcagc
     3301 acggcaataa actggattgc tctgcgatgc tttttcagca tctccagatc aaacttcaaa
     3361 attgttgcct tatctatata taacatagaa ccaccctata aaattaagaa gaaaatcccc
     3421 tgctatcaat ctatgccaaa aacgcgtcta agaatgcagt cgatttaata aaaatttcct
     3481 aattgcagta tctgatgcat ctgtaactca ttgtattgaa ataaaaatat ctgattttga
     3541 tattttccat caacatgaca tatacagaaa accaggttat aacctcagtg tcgaaattga
     3601 ttcgtgacgg ctctttcact ttatagttga ggatattacg atgaaaaaag tattaggcgt
     3661 tattcttggt ggtctgcttc ttctgccagt tgtgagcaat gcagcggatg cgcaaaaagc
     3721 agctgataac aaaaaaccgg tcaactcatg gacctgtgaa gatttcctgg ctgtggacga
     3781 atccttccag ccaactgcag ttggttttgc tgaagcgctg aacaacaaag ataaaccaga
     3841 agatgcggtt ttagatgttc agggtattgc aaccgtaacc ccagctatcg ttcaggcttg
     3901 tactcaggat aaacaagcca actttaaaga taaagttaaa ggcgaatggg acaaaattaa
     3961 gaaagatatg taattccggg aatgcgttac atcgtacttc cttgcatatt gaacaggccg
     4021 gaatatcttc tttaaaagca gctattcctc ctgttcatat ataatctcta tattgaatgg
     4081 gttacaaaat gaatatttca tctctccgta aagcgtttat ttttatgggc gctgtagcgg
     4141 ctttgtcact ggtgaacgca caatctgcgc tggcagccaa tgaatctgct aaagatatga
     4201 cctgccagga atttattgat ctgaatccaa aagcaatgac cccggttgca tggtggatgc
     4261 tgcatgaaga aacagtatat aaaggtggcg ataccgttac tttaaatgaa aacgatctca
     4321 ctcaaattcc taaagtgatc gaatactgta agaaaaaccc gcagaaaaat ttgtatacct
     4381 tcaaaaatca agcatctaat gacttgccga attaatgagg tacaagtaaa aaggagtagc
     4441 aagttgagcc atcttgctgc tcctttttgc atttttatat gacagcagaa tttattatac
     4501 gtcttatact cgcggcaatt gcctgtggcg ctattggcat ggaaaggcaa atgcgcggca
     4561 aa
//
LOCUS       DAJVRP010000193         4551 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVRP010000193 DAJVRP010000000
VERSION     DAJVRP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.195"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..1224
                     /locus_tag="OQN12_003976"
     CDS             <1..1224
                     /locus_tag="OQN12_003976"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS1656118.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1898
                     /locus_tag="OQN12_003977"
     CDS             1224..1898
                     /locus_tag="OQN12_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1656119.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2426..3187
                     /locus_tag="OQN12_003978"
     CDS             2426..3187
                     /locus_tag="OQN12_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1656120.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="OQN12_003979"
     CDS             3231..4310
                     /locus_tag="OQN12_003979"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1656121.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4551
                     /locus_tag="OQN12_003980"
     CDS             4307..>4551
                     /locus_tag="OQN12_003980"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1656122.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          864 c         1119 g         1319 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgtaggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat t
//
LOCUS       DAJVRP010000194         4501 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVRP010000194 DAJVRP010000000
VERSION     DAJVRP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.196"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            399..2678
                     /gene="ydeP"
                     /locus_tag="OQN12_003981"
     CDS             399..2678
                     /gene="ydeP"
                     /locus_tag="OQN12_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1656123.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2926..3129
                     /gene="safA"
                     /locus_tag="OQN12_003982"
     CDS             2926..3129
                     /gene="safA"
                     /locus_tag="OQN12_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1656124.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3197..3958
                     /gene="ydeO"
                     /locus_tag="OQN12_003983"
     CDS             3197..3958
                     /gene="ydeO"
                     /locus_tag="OQN12_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1656125.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4360..>4501
                     /locus_tag="OQN12_003984"
     CDS             4360..>4501
                     /locus_tag="OQN12_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1656126.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1283 a          956 c          995 g         1267 t
ORIGIN      
        1 caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg
       61 aataattgtg attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt
      121 attttgtgaa ggctattatc ctacacctgt aagaaaatcc gcgcacaatt gaagccagag
      181 tcttattttt tatattgaaa aatatgttaa tttaaacact atgagagatg atgcttaact
      241 tatcgtattt aatctattgt ttaacgataa tagattaaaa atagaaagag agtagtccct
      301 tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat catcgctatt
      361 acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa attgaatcct
      421 accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc
      481 agatggatat acgccatgat gttattgcca tgtttgacat gaataagcca gagggctttg
      541 actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac atttgtgaaa
      601 acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc tctttctttg
      661 ctgagaatac ggttcaatca ttacttacct ggggagatca cgagcttgag gctgcggggc
      721 gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc
      781 aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg
      841 aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc
      901 gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa ccgacaagcg
      961 tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga
     1021 agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct cgcatgctga
     1081 cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg
     1141 aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg
     1201 agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg gcgttgctca
     1261 aggggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca ggtcggccct
     1321 cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg
     1381 acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt cagacacaaa
     1441 tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg
     1501 ggatcactca gcacgaacat ggtacccaga acgtgcagca actggtcaat ttgctgttga
     1561 tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt cactctaatg
     1621 tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc
     1681 tgggtgagcg ctatggcttc cccccacctc atgcacctgg acatgctgca attgccagca
     1741 tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc aactttgcac
     1801 tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat ttggcggtac
     1861 acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc
     1921 cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg gtaaccgttg
     1981 aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc
     2041 tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg
     2101 tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc
     2161 tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa
     2221 atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagca
     2281 aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac
     2341 gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat
     2401 tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa
     2461 acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc tcacgccgca
     2521 tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc
     2581 cagaatcgaa tcacatgcta acacttgata accacgatcc attaagtggc attcctggct
     2641 ataaaagtat tccggttgaa ttagaaccat caaattaatg cctcttctca tttcttctgc
     2701 tgtcatccgc acagcagaag aattcctcat tgactattat tgcgcaattt gctcacatgg
     2761 attaaattaa actacatact ataagatata aacttctgcc tacagctgta agaaactccg
     2821 ctcagtactg aagcaccagt cctatttctt cttttctcca gcctgttata ttaagcatac
     2881 tgattaacga cttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca
     2941 gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgc aattgtggtt
     3001 gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca gctgagtatt
     3061 tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag tttgcaagac
     3121 tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata aaactttatt
     3181 gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc atgctttcaa
     3241 cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga tggtagataa
     3301 cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga
     3361 aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc
     3421 tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta
     3481 tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct
     3541 gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt
     3601 ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga
     3661 tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca
     3721 aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg
     3781 cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt
     3841 tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg
     3901 ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag ctatttgatt
     3961 atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg cagccattcc
     4021 ctgccccgcg ttgtttttag gcgatatatt tattgaaata aataagtgac atccatcaca
     4081 tatttatgca cttgcataac ctgttgcatg attatttatg atctcaattc ggcattttgt
     4141 cagtaaaatg caataattta ttaaatatca ataaattagt tgtttatcgg cgagaaatta
     4201 cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct ttttttagct
     4261 aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc tcttcgtaaa
     4321 attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc attaaagaaa
     4381 agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc atttgctgct
     4441 catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt ctcagacttt
     4501 a
//
LOCUS       DAJVRP010000195         4242 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVRP010000195 DAJVRP010000000
VERSION     DAJVRP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.197"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            29..910
                     /gene="yddG"
                     /locus_tag="OQN12_003985"
     CDS             29..910
                     /gene="yddG"
                     /locus_tag="OQN12_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1656127.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1165..2265
                     /gene="ompC"
                     /locus_tag="OQN12_003986"
     CDS             1165..2265
                     /gene="ompC"
                     /locus_tag="OQN12_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1656128.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2429..2914
                     /locus_tag="OQN12_003987"
     CDS             2429..2914
                     /locus_tag="OQN12_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1656129.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3024..4013
                     /locus_tag="OQN12_003988"
     CDS             3024..4013
                     /locus_tag="OQN12_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1656130.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1316 a          875 c          805 g         1246 t
ORIGIN      
        1 taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct
       61 aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct
      121 cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac
      181 ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt
      241 attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca
      301 ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat
      361 tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt
      421 cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa
      481 tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc
      541 ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct
      601 gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt
      661 tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatac
      721 tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt
      781 tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc
      841 gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg
      901 tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa
      961 tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc
     1021 acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc attctatttt
     1081 aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa
     1141 cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt
     1201 ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg taataaactc
     1261 gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg tgacgatggt
     1321 gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt
     1381 ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa cggctcgcgc
     1441 ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg ctctctggac
     1501 tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat gttgccagaa
     1561 tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac caccggtcta
     1621 gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt tgccgcgcaa
     1681 tatcaaggca aaaatgaacg tactgacaac agtcatcttt atggtgctga ctacacgcgt
     1741 gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt tggtatcggt
     1801 gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc taatcctctc
     1861 aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga tgccaacaac
     1921 atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg ggatggttat
     1981 atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga cttcggcttg
     2041 cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta cgccgaccag
     2101 gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc
     2161 tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc cgtagatatt
     2221 cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc tctcctgacc
     2281 agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt aagtaaaaat
     2341 gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat atcctccggg
     2401 gacatcttac tataatattt attgatgagt gattatgacc aatattaata cagcttgtgt
     2461 aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa ccatctccag
     2521 caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg gtgaagaaag
     2581 agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat ctcttgattt
     2641 gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata cgttaaatct
     2701 ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg ttaatatcaa
     2761 cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac tggaaaacat
     2821 ttatattgat cataacaatc tggaaagttt agatttaaaa aatcagcatt cattggttaa
     2881 tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat aaactgaaat
     2941 ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa gaatctctga
     3001 ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat catcccagga
     3061 tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa aacactacaa
     3121 aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac attgatcaac
     3181 taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat gatccacttg
     3241 cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat gaagtgacca
     3301 ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac ttcacctcta
     3361 tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat agactcgtaa
     3421 agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc agaaatgtaa
     3481 gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata gataggcttt
     3541 catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa ttagaatctt
     3601 gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt gttgcaggta
     3661 ataaaaatga actcttactg ctggatctat cccataataa actaacaagt ttacacaatg
     3721 tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct gaaattaaaa
     3781 tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac cagttgaaat
     3841 atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga ctggacaata
     3901 ataaaataac ccacattgat actaataata catccgatat tggaacttta ttccccataa
     3961 taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat tgacctacta
     4021 tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact ggcctggcaa
     4081 ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcggat aacgaattgt
     4141 ttcactatac tattaaaaaa gcatataccc catataatat acacacttac aagacagagg
     4201 aagttgtaaa ccagaggaat ataaaagtta aaaacatgac ct
//
LOCUS       DAJVRP010000196         4183 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVRP010000196 DAJVRP010000000
VERSION     DAJVRP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.198"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQN12_003989"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQN12_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1656131.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OQN12_003990"
     tRNA            complement(324..399)
                     /locus_tag="OQN12_003990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OQN12_003991"
     tRNA            complement(406..480)
                     /locus_tag="OQN12_003991"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OQN12_003992"
     tRNA            complement(597..681)
                     /locus_tag="OQN12_003992"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OQN12_003993"
     tRNA            complement(690..765)
                     /locus_tag="OQN12_003993"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OQN12_003994"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OQN12_003994"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1656132.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQN12_003995"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQN12_003995"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1656133.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQN12_003996"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQN12_003996"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1656134.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1081 a         1039 c          929 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcat ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DAJVRP010000197         4110 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVRP010000197 DAJVRP010000000
VERSION     DAJVRP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.199"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(954..1145)
                     /locus_tag="OQN12_003997"
     CDS             complement(954..1145)
                     /locus_tag="OQN12_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1656135.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="OQN12_003998"
     CDS             1209..1532
                     /locus_tag="OQN12_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCS1656136.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="OQN12_003999"
     CDS             1522..1833
                     /locus_tag="OQN12_003999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656137.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2230
                     /locus_tag="OQN12_004000"
                     /pseudo
     CDS             1808..2230
                     /locus_tag="OQN12_004000"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2211..2741
                     /locus_tag="OQN12_004001"
                     /pseudo
     CDS             2211..2741
                     /locus_tag="OQN12_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2748..2981
                     /locus_tag="OQN12_004002"
     CDS             2748..2981
                     /locus_tag="OQN12_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1656138.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3527..3994)
                     /locus_tag="OQN12_004003"
     CDS             complement(3527..3994)
                     /locus_tag="OQN12_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656139.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT         1001 a          938 c         1194 g          977 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcactatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc gggatagacg cgctttacca tgccggagag ataaaaggcc gtcaggatgt
     2101 gattcaggct ctcacagagg cagggattga cgtggttagg gtgacgcgaa gcagtatcag
     2161 cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcgtttt atgagcaatc
     2221 ttttacagac gggtgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2281 aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aaacagggaa ctgacctcaa
     2341 acgaatcttt aatcaggaac gctatcaacg accatacatc agccctgaaa gaacttctgg
     2401 agaggtatca gaaaacgacc gtcgatacga tggacgcaca ctggaaaacc gtactgaaaa
     2461 tgagcgtgaa acgctggctg tggctgatta tcgtctcagt actgatgttt gccacgaccg
     2521 ggagcctgct gtggtaccag ggcatgaaga taaacgcgaa catgaatatt ctccgcgagt
     2581 agaaagagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgaggaca
     2641 gcaacggacg ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg
     2701 aaaacgggaa acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg
     2761 aaagacattt gctgaacgca ttagaacaac tgcaacagga ctatatgcag cggctgaacg
     2821 aatgggagag cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg
     2881 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     2941 cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca
     3001 ggaatgcgaa catgcgttat ccctggagcg tgagcaccag ctggaaatac aggaacggac
     3061 actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt
     3121 tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag
     3181 accttgctga aaacatagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt
     3241 ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttcttttttc
     3301 ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc
     3361 tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt
     3421 taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     3481 ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagatattac gttaaattgt
     3541 gagggaatgg tccccatata ctgactttat catagggata gggttggtag tgggtcgatt
     3601 gggtttcggc attgtttccg ttaatgccaa ttgcattttt gtatttgatg caacagaagg
     3661 cgtgagattc tcctgggcct ggaaaggata agtgtttaga attgatggca aaatatcttc
     3721 catccgattt ttgaagtagt tgttgtttgg ctgtctccca aagggtgttg ttacaaactt
     3781 cttgaatttg tagtttttgc aggactctta ttactgcgtt atcatgttcc aattctttag
     3841 gggagtgtat tattctctct tttaataagc tgtttaccac agggtttagt tgttgtctaa
     3901 agcacgcaga taatgccatt gctacacaat tttgttcccg aggaagcatg tttggcgggg
     3961 tatgccgtgt ttgatgtgat atgtctattc tcatagaagg tctctggctt atagcatcaa
     4021 caaatacggc tataaaacaa aattttcata gagtgattca agatcttctt gagatccttt
     4081 tttgcggcgc gtaatctgct gcctgcaaac
//
LOCUS       DAJVRP010000198         3997 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVRP010000198 DAJVRP010000000
VERSION     DAJVRP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.200"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            38..643
                     /gene="gstA"
                     /locus_tag="OQN12_004004"
     CDS             38..643
                     /gene="gstA"
                     /locus_tag="OQN12_004004"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1656140.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(687..1550)
                     /gene="pdxY"
                     /locus_tag="OQN12_004005"
     CDS             complement(687..1550)
                     /gene="pdxY"
                     /locus_tag="OQN12_004005"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1656141.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1609..2883)
                     /gene="tyrS"
                     /locus_tag="OQN12_004006"
     CDS             complement(1609..2883)
                     /gene="tyrS"
                     /locus_tag="OQN12_004006"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1656142.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(3012..3668)
                     /gene="pdxH"
                     /locus_tag="OQN12_004007"
     CDS             complement(3012..3668)
                     /gene="pdxH"
                     /locus_tag="OQN12_004007"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1656143.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3727..>3997)
                     /gene="mliC"
                     /locus_tag="OQN12_004008"
     CDS             complement(3727..>3997)
                     /gene="mliC"
                     /locus_tag="OQN12_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCS1656144.1"
                     /translation="GQIQVERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHL
                     KQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
BASE COUNT          971 a         1063 c          975 g          988 t
ORIGIN      
        1 ctaccatact ttaaaccaca gacaaaagga gttaccgatg aaattgttct acaaaccggg
       61 cgcctgctct ctcgcttccc atatcaccct gcgtgagagc ggaaaggatt ttacgctcgt
      121 cagtgtggat ttaatgaaaa aacgtctcga aaacggtgac gattactttt ccgttaaccc
      181 taaggggcag gtgcctgcat tgctgctgga cgatggtact ttgctgacgg aaggcgttgc
      241 gattatgcag tatcttgccg acagcgtccc cgaccgccag ttgctggcac cggtaaacag
      301 tatttcccgc tataaaacca tcgaatggct gaattacatc gccaccgagc tgcataaagg
      361 tttcacacct ctgtttcgcc cagatacacc ggaagagtac aaaccgacag ttcgcgcgca
      421 gctggataag aagctgcaat atgtgaacga ggcactgaag gatgagcact ggatctgcgg
      481 gcaaagattt acaattgctg atgcctatct gtttacggtt ctgcgctggg catacgcggt
      541 gaaactgaat ctggaagggt tagagcacat tgcggcattt atgcaacgta tggctgaacg
      601 tccggaagta caagacgcgc tgtcagcgga aggcttaaag taatagccat cggcccacag
      661 gtcgttgtgg gccgaaatga gatatttcag agctttgttg cgctgaagta atgttctggt
      721 ttggcaatac gatcctgagc agccaccact tgcagctcat attcctgcat tgctttggtg
      781 gtcaccatga tttcgtagac tgcagcggtc acatgttcca gggcctcctg cagcgttgcc
      841 ccctgaagca gtttcaccag cagtaaaccg ctcgtcacat caccaacacc taccggctgg
      901 cgcataccaa aatccaccag tggacggctg atatgccaga cttcatcggc ggtgaccagc
      961 agcatttcaa aacggtcacg gctgtagcca gctcgcgcca ggtgtttaac caacacaatt
     1021 tgtggccctt gctcaatgag ttcgcgcgct gccagaaccg cttcttcgac gttatttacc
     1081 gcatgctcac agagtatttc cagctcaacc agattcggcg caatgatatc gctggcaggc
     1141 aaaccgtgcc gcacatgaaa ctctgcgaca cccggtgcaa cgatacagcc tttttccgga
     1201 tgacccatta ccggatcgca aaaatatttc gcctgcggat tcgctgcttt cacctgacgg
     1261 acgataccga ggatatgttc accctgctcc gccgatccca gatagccact taatacggca
     1321 tcacaggtgt gtaatttatc aatggcggca atgccttgca caatttcggt taaatggctg
     1381 ggcggcatca cgcagccagt ccatttgccg tattgggtgt gattagaaaa ttgaacggtg
     1441 ttcagcggcc agacgttcgc gcccaggcgg cgcatcggaa actctgccgc actgttaccc
     1501 gcatgaccat aaacaacgtg agactggata gcgagaatat ttttcatcat gttccctgta
     1561 taaaaaccag ggggagtgat ttctcactcc ccctttccac ttaatgcatt atttccagca
     1621 aatcagacag taattctttt tgccgcgacg cagtaaggta aaacgaccaa acagacgatc
     1681 ttcttcttta aagaagtatt caggatcgga ctgtttttca ccgttaatgg tgatggcatt
     1741 ggaggcgata gttttacgcg cctgaccacg ggaaggttgc agttcagaat cgaccagcgc
     1801 ctgcatcagg tctgcgccct tttccatctc aaccatcggt acgccgtcct gcgccagctg
     1861 ttcgaagtcc gcttcactca gcgcactcaa agaaccgctg aacaggcatt cggtaatacg
     1921 ttttgccgcc tgtaaacctt cttcaccgtg aaccagacga gtcacctgct ccgccagtac
     1981 atactgggcg cgcggtgctt taccgctgtt tttatcttct tcttctaggg cgttgatctc
     2041 ttcaatgctc ataaaggtga agaacttcag gaagcggtaa acgtcggcat ccgcagtgtt
     2101 gatccagaac tggtagaatt tgtacgggct ggttttcttc ggatccaacc agactgcgcc
     2161 gccttcagtt ttaccaaatt tggtgccgtc tgctttggtg atcagcggaa cggtcaggcc
     2221 aaacacctga ttctgatgca gacgacgggt caggtcgata ccagaagtga tgttacccca
     2281 ctggtcagaa ccaccaattt gcagcaccac atcgtactgt ttgttcagac aggcgaagtc
     2341 ataaccctgc aacaggttgt aggaaaactc agtgaacgaa atcccctgat cttcacggtt
     2401 gagacgctgc ttaaccgctt ctttgttgat catctggtta acggagaagt gtttgccaat
     2461 atcgcgcagg aaggtcagca cattcatatt gccgaaccag tcatagttgt tcgccgcgat
     2521 agcagagttt tctccacagt cgaaatcgag gaacggggca acctgcttac ggattttgtc
     2581 cacccactcc tgaacagttt cttcggtgtt cagcttacgc tcggcagctt tgaagctcgg
     2641 gtcgccaatc agacccgtcg cgccgcctac cagcgcaacc ggcttgtggc ccgcctgctg
     2701 gaagcgtttc aggcataaca atggaacaag atgccccaaa tgcaagctgt cagcggtagg
     2761 atcgaagccg caatagagcg cgatcgggcc ttgcgccagt cgctctgcta acgcttcctc
     2821 gtccgtcacc tgggctacca gcccccgctc ttgcaattgt ttaatcaagt tactgcttgc
     2881 catcaaaatc tccatgtata taacgactgc acctttgccg gtacacgact tttcgccaga
     2941 tgcgaaagag acatagaata aagtgccaga atcaggagta ccagcgatta aagcaagatt
     3001 tttgcatctt ttcagggtgc aagacgatca atcttccacg catcattttc acgctggtac
     3061 aaaaagcggt catgcaggcg atgctcaccg ccctgccaga actcaatctg ttcaaggctg
     3121 acgcgaaaac cgccccaaaa gctcggcaat ggcacttcgc cctgttgaaa cttctgcttc
     3181 agctccagga atttactttc aaggataccg cgggcagaaa tgcgactgga ctgcttcgaa
     3241 acccatgcac caatctggct atcacgcggg cggctatgaa aatatttcat cacttcgaga
     3301 gtcgaaagtc gttctgcttt accgatcacc atcacctggc gttcaagggt atgccacggg
     3361 aacagcaggc taacgcgcgg attattttca atttgatgtg ctttacggct accgaggttg
     3421 gtgtaaaaca ccatgccttt ttcgtcgtaa tgtttgagta aaacgatgcg ctgataaggc
     3481 tgaccatgtt catccacggt agcgaccacc atcgcggtag ggtccgccag tttggcttca
     3541 caagcctgag agagccaacg ttcaaaaagg gttaatggat cggcgggaag atcgcggcgg
     3601 cgtaacccgc ctttggtgta ttcacggcgc agatgcgcga tttgctgcaa ttcgtcgtta
     3661 tcagacatgg ttttctttac ggattgtcag tgggtgacgc tattgtgcgc cgcccctgga
     3721 aaaatctcaa cgctgtggat tttgtaactg acagttattc aagacgatgc ggtcgcgttt
     3781 atagacagtc gcttcctcac ctttcgacca gaaaacatag atcccgtcgg agtaacgcgc
     3841 gccagaggct gaaaggccct gtttgagatg cagtagttga ttatcgtaaa caaaactgac
     3901 ttcctggcgc ggattattca gtttgaccgt caacggtttt tcatcacact ggtattccag
     3961 cgtatcggtt tgcattcgct caacctggat ttgcccc
//
LOCUS       DAJVRP010000199         3945 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVRP010000199 DAJVRP010000000
VERSION     DAJVRP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.201"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..357
                     /locus_tag="OQN12_004009"
     CDS             <1..357
                     /locus_tag="OQN12_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1656145.1"
                     /translation="VFVHTKPAYRSDGIKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="OQN12_004010"
     CDS             619..981
                     /locus_tag="OQN12_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCS1656146.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1898
                     /locus_tag="OQN12_004011"
     CDS             978..1898
                     /locus_tag="OQN12_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1656147.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1901..2852
                     /locus_tag="OQN12_004012"
                     /pseudo
     CDS             1901..2852
                     /locus_tag="OQN12_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2879..3119
                     /locus_tag="OQN12_004013"
                     /pseudo
     CDS             2879..3119
                     /locus_tag="OQN12_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3116..3421
                     /locus_tag="OQN12_004014"
     CDS             3116..3421
                     /locus_tag="OQN12_004014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656148.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3655..>3945)
                     /locus_tag="OQN12_004015"
     CDS             complement(3655..>3945)
                     /locus_tag="OQN12_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1656149.1"
                     /translation="SQSVTKMITCGENGHPVIVDRPSPTPEQLALINNEKKSALIAEA
                     TNVIAPLQDAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
BASE COUNT         1143 a          772 c          765 g         1265 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa taaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tctcaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct ataccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatc gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaga
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtcagac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggtgaat atataggtag aatttactta gaaacaaaat
     1861 caagacctaa gtatattttg aagaaaggta gaaaataacc atgagcatat gtattaaaca
     1921 atcagcatta aaaatattat tggctttatc agcactccta ataacttggt tgactaccag
     1981 atactttcca gttgaaccgg atgttgctaa cagcccaatt gtatggaggc atattttaga
     2041 gaatggtata tcatcaatcc acgactggaa accaaccgtg gataattggt atttcaccgt
     2101 ttacccaatt aactttttat tttatatgct gttaggtgat gatggcttag tagcattaag
     2161 attatcaact gcagttttct ctattgctat agtaatagca gcgatgttaa cgctacgtaa
     2221 ggcattcggg tttacccctg ctttattttc tattatactt ctatctctaa taccatattt
     2281 ttcatacact tatggttttg tatcacatcc tttttctcat aattcaacaa acgctttcgg
     2341 ctttttatgc ttgcttatat ctgttttcaa catacagtat aaaaatatat ttatcactct
     2401 cctcttaagt ttgacagcat tattttccag cgtatccgat ccgtggttta cagctgcatt
     2461 ttttattcca ctattaatat cttacttttt attctctgta tgggataaaa aattatttaa
     2521 gcatactgca ttaatactat ttgcatgttt aatctctctt tccaatgtat tgcaaaatct
     2581 acttaacata cctccccacc aatttgaaat tgtatcactc aatgatatga ttttaaatgc
     2641 aaaatggtgc attctgttaa taggaaaaag tcttaattta ttagttgtcg acaataacgc
     2701 aacatcttac gcctcatttg tcatttggtt cattgccatt attacatctg cgtggtttgt
     2761 tttatcggac aataaaaaaa acacataccg tatttatatt gtgttatttt cattattatc
     2821 tatcgcaggt attgtctcat cattcatact gagctataaa tcccctgatt atattagcat
     2881 gcgtttcttt atgaatgtta cctgtttcgc actgatattg tgctgtatcg gtacatcaac
     2941 aaaagcaaag atattatttt atctaatcgc atttttattt tcgatcagtt caattaaatc
     3001 ttacacaaat aatgcttcac cattacacga tcaggagaag attgttaaat catacattga
     3061 ttttttaaaa aaaaataatc tccattatgg gtacggctct ttttgggatt tgtcaatgac
     3121 tgtaaattgg ttatctggtg gggacataca gataactcct gttttcttta atgctgattc
     3181 ggggaaaatt aactttactg gcgtgagaca acagacactg gcttcttggc atagcaagga
     3241 ggcgttcaat tctgctcctg agcgacagtt tatagcagtt tccatagcta acgaacctga
     3301 acgatgtaaa gaaatgacat cctgccttgc aggtattcaa gaacaattag gtaagcctga
     3361 cgaagttttg aactttgaag gaagagtaat attagtattt aacaaaaaac ttaacctata
     3421 gtaaattgcc ccctaccata taaaccacac taacgaatga ataacccgtc tttttcgacg
     3481 ggttatatcc ctatcaggaa taaaaatgga gtggttgccg tatacaacaa accctcattt
     3541 agagctatat tctagagtat atgagatact ataaagttga aggagctatc attagaaaaa
     3601 ccatatgctg ttataattag atgtctcgct tttcatccaa taacgaattt tctcttatag
     3661 attgctctct ggttttttcg gccactctgt attttttata tcaacacgca tcaatagtgc
     3721 gcgatatttt ttccatgcca gtaacagttt cgtttcatca tctgttgcca tatctaaatc
     3781 aaccgcatcc tgaagcggcg ctattacatt cgttgcctct gctatcagtg cagatttctt
     3841 ttcattattt attaaggcta attgttctgg tgttggagaa ggacggtcaa caatgacagg
     3901 gtgaccattc tcaccacagg taatcatttt tgttacactc tgcga
//
LOCUS       DAJVRP010000200         3865 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVRP010000200 DAJVRP010000000
VERSION     DAJVRP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.204"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..471)
                     /gene="cybB"
                     /locus_tag="OQN12_004016"
     CDS             complement(<1..471)
                     /gene="cybB"
                     /locus_tag="OQN12_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS1656150.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALA"
     gene            660..1661
                     /gene="gap"
                     /locus_tag="OQN12_004017"
     CDS             660..1661
                     /gene="gap"
                     /locus_tag="OQN12_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1656151.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(1703..3142)
                     /gene="aldA"
                     /locus_tag="OQN12_004018"
     CDS             complement(1703..3142)
                     /gene="aldA"
                     /locus_tag="OQN12_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCS1656152.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3339..>3865)
                     /locus_tag="OQN12_004019"
     CDS             complement(3339..>3865)
                     /locus_tag="OQN12_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCS1656153.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
BASE COUNT         1017 a          991 c          892 g          965 t
ORIGIN      
        1 tgccagtgca gccgcagcgt gcaacccgat gacaaaatat cccagattcg ccagcgtctc
       61 atgccacgac tttaagctat caacccgttc gaaattggcc tctgaagcgt aaggcatcgt
      121 caaaccaaac gcaaaccacg ggttgccccg gttatacatc atcaccaaac caatcactgg
      181 cagcgcaata aacaggagat aaatcaccaa atgtcccaaa tgcgccagtc ccgtcatcat
      241 cggtttcggc ttaggtataa tcggcggggt tgggtatttc agtcttaaca aaagacgaac
      301 caccatcagc acgagaattg agatgccaca ggaaacatga atcatgttga tgagcggccg
      361 atcgctacgt gggaagaaac cacgaaactc cattgcgcaa tacgctgcga taaccagcaa
      421 aaagaccagc cagtgaatgc tgatttgtaa ccttgagtat ttattttcca taacatttcc
      481 tgctttaaca taattttccg ttaacataac gggcttttct caaaatttca ttaaatattg
      541 ttcacccgtt ttcagtacgt tacgtttcac tgtaagaatg gagattgccc atcccacaat
      601 cctggtctaa gcctggaaag gatcaatttt catccgaacg ttcctgacag gagaaaacca
      661 tgagtaaagt tggtattaac ggttttggtc gtatcggtcg actggtgttg cgtcgtttac
      721 ttgaagtcaa aagcaacata gacgttgtcg ctattaatga tcttacttcc ccaaaaattc
      781 tcgcctatct gctgaaacat gattcaaact atggtccgtt cccctggagc gttgatttta
      841 cggaagattc acttatcgtt gatgggaaaa gtatcgcggt ttacgccgaa aaagaggcta
      901 aaaatattcc gtggaaagcg aaaggggcag aaatcattgt cgaatgtact ggcttttata
      961 cctccgccga taaatcgcag gcgcatcttg atgctggtgc gaagaaggtg ttgatttccg
     1021 ctcctgccgg tgaaatgaaa accatcgttt ataacgttaa tgacgacact ctggatggca
     1081 acgacaccat tgtttccgtg gcgtcatgca ccactaactg ccttgcgccg atggccaaag
     1141 ccttgcatga cagtttcggg atagaagtcg gcacgatgac gaccattcat gcctatactg
     1201 gcacccagtc actggtggat ggcccgcgtg gtaaagattt acgtgcttca cgcgcagcgg
     1261 cagaaaatat cattccccac actacggggg cggcaaaagc cattggtctg gtgatcccgg
     1321 aactgagcgg caaactgaaa ggtcatgcgc aacgcgtgcc ggtgaaaaca ggttcggtca
     1381 ctgagctggt gtccattctc ggaaaaaaag tgactgccga agaggtgaat aacgcactta
     1441 aacaagcaac caccaataac gagtcatttg gttataccga tgaagaaata gtctcttccg
     1501 atatcattgg cagccatttc ggttcagtgt ttgatgccac gcaaacggaa attaccgccg
     1561 tgggcgattt acaactggtg aaaacggtcg cctggtacga taacgaatat ggcttcgtca
     1621 cgcagcttat tcgcaccctc gaaaaattcg ctaaactctg acgcgcacag gcggaggaaa
     1681 aaacctccgc ctctttcact cattaagact gtaaataaac cacctgggtc tgcagatatt
     1741 catgcaggcc atgtttacca tctgcgccgc caataccgga tttacgccat ccggcgtgga
     1801 aaccttgcat agcttcgaag ttttcacggt tgatgtaagt ttcaccaaac ttcagccctt
     1861 taatggcttt catcgcgacg ttcagatttt gggtatagat tgatgaggtc aggccgtaat
     1921 cactgtcatt agccattgag atagcatctt ccagcgtgtc aaatgcgaca actggcagca
     1981 ccgggccaaa ggtttcctca tgcataatcg acatttcctg gcgaacatcc agcagcaatg
     2041 tcggcggata ataatatcct ttcccctcta ccgctttgcc acccaacgcc actctcgccc
     2101 cttcttctac tgcgcgcgcc actttttgct caaccctttc cagcgccgcg gcgttaatca
     2161 acggccccat cgcaatgtcg ttgcgttcag cggggttacc aaattgaacc gcctgcaacg
     2221 cttcacccag cagattgacg aactgatcat aaatgccttt ctgtacataa acacgttctg
     2281 cacagttaca cacttgccca ctattaatga cgcgtgaatc aacgatggct ttgactgcca
     2341 gttcaagatc ggcatcgtcc attacgatag ccggtgcttt accacccaat tccagacaca
     2401 ctttggtgat gtttttcgcc gcagtcgcca tgatcttctc acctgcagag acgctgcctg
     2461 tcatactgac cattgcgacc tttgggttac ccgccagttc ttgcccaacg gtttcaccac
     2521 gccccagtac aaggttaaac acgccgcgcg gaaggcctat ttcatcgacg attttggcga
     2581 atgcaatcgc attgtttggc gtaaattcac tgggtttaat gacgatggta ttaccggtca
     2641 aaagagcggg agccattttg cgggcaatga ggaagaacgg aaagttccac ggcagaatgc
     2701 cggtagtcac accaagcgca cgtttaaaca gaagaatatt ttctcccgga cgatcgcttt
     2761 gaataatctc gccctcgtaa cgccgtgccc actccgccat gtaatcgata tagtcggcag
     2821 taaaagccac ttcgacttca gctagctgct ggatcttgcc cccttcttca acaatcagcg
     2881 cactgatttc actggcgcgt tcgcggatcc cggcggagat tttgcacaac caactggcgc
     2941 gttcaatagc aggcaacgct tcccattctg gttgtgcacg ttctgctgca tcgattgcct
     3001 tacgggcatc ctcggcctga ccatcgggta tgcgggaaat gacagcctct gtagcagggt
     3061 ttaccacatc aatccatgcg tctccacgcc aggtaacaaa ctgtccatcg atatacatag
     3121 gatgttgaac gggtactgac atgggcgact cttgtgattt atatgtttgt tttcggtgaa
     3181 tatattgtta attaacatca atcatcagag agacggcatc gggaaaggac aagtctgtgg
     3241 agggcttcat aaaaacaagt gtaaaaggaa taaaaggcag aaataaaagt caggagtcat
     3301 gcccgtttgc aaacaagtgg ttcacaaacg ggcaattttt aacgtaacga gcgactttcc
     3361 atcgcctcga tgagcaccgc atcatgtttc agcgtttgcc atgcatggat gacttctgcc
     3421 ggaaaatcaa cgtgaacgat aaaatctcgc ccgcggggac cgtagccatc gctatcatcg
     3481 tgtaaacgcg gcagctcgcc agtgagtagt gagagataac gctcaaccgg ccataatcct
     3541 tgtaactgat taataaagat tacactgtct gcgttatttc ctaactgagg aacgaatccg
     3601 ggataactta accagcgtgg tgcatcggga ttgtccccgg tcatacggcg gaacgttgcc
     3661 atcgtgcgcc gctgcatggt ggggtcctgt acaacgatag ccgtatgaac tcgttctacg
     3721 gcctgattca atagcgtgat gctaaagcgt gcgttttcac cgcagtttgt tgactggtct
     3781 tcaatccaga ttttttcatg cggaatatgc cagaactgat gagcgatatc caccaggatg
     3841 gtcgcttctg ctctgccagt ggtgc
//
LOCUS       DAJVRP010000201         3858 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVRP010000201 DAJVRP010000000
VERSION     DAJVRP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.205"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..328)
                     /gene="flhe"
                     /locus_tag="OQN12_004020"
     CDS             complement(<1..328)
                     /gene="flhe"
                     /locus_tag="OQN12_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCS1656154.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVP"
     gene            complement(328..2405)
                     /gene="flhA"
                     /locus_tag="OQN12_004021"
                     /pseudo
     CDS             complement(328..2405)
                     /gene="flhA"
                     /locus_tag="OQN12_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2398..3545)
                     /gene="flhB"
                     /locus_tag="OQN12_004022"
                     /pseudo
     CDS             complement(2398..3545)
                     /gene="flhB"
                     /locus_tag="OQN12_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          958 a         1130 c          941 g          829 t
ORIGIN      
        1 ctggcacttc ccagataaat cgcaacggtt ctgctgccgc tataccggaa aaggccacgg
       61 tggtaccgct ctgcccctct aattcgacac aacgagattg cgaacacaag cgaacccgca
      121 gtcctgcagg tgtcgggccg ataagctgat aacgccacgc caccagcgtc atcaatcctg
      181 aagcaggttg tcgcgcagaa agaggtgcag acgacatcga ctcaccgcga tgatttagcg
      241 taattccgac actacttgcc tgccacatcc cctccccggc ggcttgcacc agcagcggaa
      301 acaataatat cgctaataag gttctcatca tttaccgcca attgtcgccg tcatgcggat
      361 atgtcggtta tcagacagtt ccagattcga caggaccact aactgcggca agctgcggcg
      421 caggaagcga gacaataatg gtcgcagcgc gtggttcacc aacaatactg gcggcgcacc
      481 cagcatctcc tgacgggata gagcttcctg agtttgcgcc agtaaacgat ccgccagccc
      541 tggctccagt cctcccccgc cctgcagcgc ctgtagtaac aaacgttcca gcggtgtatc
      601 gaggccaata acatggactt catctttgcc aggaaaccac tgctgggtaa tcgcccgtcc
      661 caacgccacg cgcacgacgg cggttaattc atgtggatcg ctttggatgg gcgcatgttc
      721 cgccagcgtt tcgagaatgg tgcgcatatc gcgaatcggt actttttcat cgaggagatt
      781 ttgcagcact ttatgcagtg tggtgagcgt gacgacgcca ggaacgagat cttccgtcag
      841 ctttggcatc tcctgggcga cgcgatccaa cagctgttgc gcctcctgac gaccaaacag
      901 ctctgcggca tgctggctaa tgaggtggtt aagatgcgtt gctaccaccg tgctggcctc
      961 aaccactgtg tacccctgaa tctgcgcctg ttcttttagc gcactttcaa tccagatagc
     1021 attcaggcca aatgccggat cgacggtcgc ctcaccaggt aacgtcccgg cagcggttcc
     1081 agggttaatc gccagccagc gccccggata agcatcacca ctgccaatct ccacgccttt
     1141 catcaaaatg cgatagcggg caggttgcag atccatattg tcgcgaatgt gcaccactgg
     1201 cggcagaaat cccatctcct gggcaaattt cttgcggata ctgcgtatac ggcccaacaa
     1261 ctcaccatcc tgctggaaat cgaccatcgg gatcagtcga taacccactt ccattcccag
     1321 agaatcttcc agttgtacat cgttccacgt cgcttcgaca acggtattat tctctgccat
     1381 ttttaccggt ttgggttcgg caggcgcttt ttgttcgcgt ccgcgtatcc accaggccag
     1441 ccccagcaat ccggcagtga acagcaaaaa taccaggttc ggcattccag gcaccaggcc
     1501 gagtaaaccg agcacggcgg cgcttaacaa cataacgctt gggttactga aaagctgatt
     1561 caccatctgc tcgccaacat cctgatcggt gctgacacgc gtaacgatgc cccccgcggc
     1621 ggtagaaatc accagcgccg gaatttgtgc caccagaccg tcgccaatgg tcaatagcgt
     1681 ataactttcc gccgcgtgtc ccatgctcat gccagttgca gcacgccgac cagcaacccg
     1741 ccgacaacgt taatgaccat gatgaggatc ccggcaatgg catcgccgcg aacaaactta
     1801 cttgccccgt ccattgagcc gtaaaaatcg gcttcctgag tcacttcgga gcggcgtttt
     1861 ttcgcctcat cttcaccaat caatccagcg ttaaggtcgg cgtcaatcgc catctgctta
     1921 cccggcatac catcgagaac aaagcgcgca cccacttctg ctatacgccc ggcacctttg
     1981 gtaatgacca taaagttgat gatcacgaga atgacaaaca ccacgatacc gatagcgaaa
     2041 ttgccaccaa cgaggaagtg accgaacgct tcgaccacct tcccagccgc cgccgcgccg
     2101 gtatgccctt ccatcaaaat gatacgggtt gaagccacgt taagtgccag acgcaacaac
     2161 gtagtaaaca acagaatggt cggaaacgca gcaaactcaa gcgtgcgctg ggtaaacatc
     2221 gccaccagca acaccatgat cgacaaggca atattgaagg taaacaacag gtcgagtatg
     2281 aatgcgggca gtggcagcac catcatcgac aagatcaaca ggatcaaaat cggtccggca
     2341 aggatctgcc attatgtcga tttcaggttt gcgggcaggc gcagcatcgc ggccagatta
     2401 ctcatgggtc ggtttctcgt taataaaatc cagggctgcc ggcaccggaa gatgagtagg
     2461 tgtacagggc gctgtccacc agccagacgc cagcgtttca gttgccagac ccaggccagc
     2521 acttccgcca ccgcggcgta cagttgaccc gggatttgtt gaccaatctc cgcatgtcga
     2581 tacagcgctc gcgccagcgg cggcgcttca agcgtcggga cgttattttc agcgccaatt
     2641 tcacgaatgc gcagcgcgac cagccctgca cctttagcga ccactttcgg tgcgctcatt
     2701 ttgttttcgt catactgcaa cgctaccgaa tagtgggtcg ggttattgac aatgacatcc
     2761 gctttcggca catcggccat catccgacgc cgtgcggcag ctcgctgcat ctgacagatc
     2821 cgtcctttaa catgggggtc gccttcgcct tgtttgaact catcacgaat atcctgccgc
     2881 gacattcgca gctttttcag gtggctgaag atttggaaaa agacgtcaaa tcccaccatt
     2941 ggaatgacac caagcaccac cagcagtgcg catagcccta ccaaatccat cgcattaccc
     3001 atggcggtaa tcggtgactc ggccattaag cgcatcatct gcggccagtg atgccagaga
     3061 aaaaaccccg tcacgctgcc aaccaggatg gttttcaaaa ttgccttaag caactccgcg
     3121 ctagtctgtg ccgagaatat ccgtttaatg cccggtagcg ggttgagttt ggaaaacttc
     3181 ggctgcaagg atttgccgct aaataccagc ccccccagca taaccggaga aatgagcgcc
     3241 accagcacca cgccgctaat cagcggtagc agcgccagca tggcttctct gatcagcaga
     3301 ataatttgcc cgaggatcag attcgggtct ttgataatac tgtgatcaaa atgcagccca
     3361 gcggagagca tgcccgacaa tcgacgggcc agcgacacac cgccaaacca gataacacta
     3421 acgcccacca gcaaaatcag cagtgaggtc agttcacggg aacgcgggat ttgcccctct
     3481 tcccgcgctt tttctagtcg gtgaggtgtg ggggcttctg ttttgtcgtc gctctcgtca
     3541 gacacgtcgc caatcctgga aagagtatta agcgtaaatg atgccagagc gcaaagcgtt
     3601 caatggttta agtaaggggc aaaacaggcg ggatttaggg cgtttatgca aaaataggtt
     3661 cagttgcaga acgttttgcg aaccattttc cagttctgat atttagccat ttagcctata
     3721 gaatcgcact ccgcctctct gttagtttgc taataattaa attattacaa aggtgatcaa
     3781 atggaatggc aacaattgct taactgcaac cgtcggaaag ataatctaaa aaaaaccaac
     3841 atagcatttt tacatccc
//
LOCUS       DAJVRP010000202         3590 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVRP010000202 DAJVRP010000000
VERSION     DAJVRP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.207"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            32..493
                     /locus_tag="OQN12_004023"
     CDS             32..493
                     /locus_tag="OQN12_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCS1656155.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="OQN12_004024"
     CDS             complement(534..1004)
                     /locus_tag="OQN12_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS1656156.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="OQN12_004025"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="OQN12_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS1656157.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="OQN12_004026"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="OQN12_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1656158.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          953 a          926 c          817 g          894 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag
//
LOCUS       DAJVRP010000203         3540 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVRP010000203 DAJVRP010000000
VERSION     DAJVRP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.208"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..455
                     /gene="pgrR"
                     /locus_tag="OQN12_004027"
     CDS             <1..455
                     /gene="pgrR"
                     /locus_tag="OQN12_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1656159.1"
                     /translation="EMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAI
                     NLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVER
                     AIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            792..2405
                     /gene="mppA"
                     /locus_tag="OQN12_004028"
     CDS             792..2405
                     /gene="mppA"
                     /locus_tag="OQN12_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1656160.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2456..3487)
                     /gene="ynaI"
                     /locus_tag="OQN12_004029"
     CDS             complement(2456..3487)
                     /gene="ynaI"
                     /locus_tag="OQN12_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS1656161.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          771 c          816 g          947 t
ORIGIN      
        1 gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca atggctattg
       61 ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca caattaatag
      121 atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc tggagattaa
      181 tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg aatacgatag
      241 acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct tatgatcagg
      301 ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc actccagatt
      361 tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca ttctcattat
      421 ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata cattaattta
      481 attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt tattcttcgt
      541 acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc gaagcccgaa
      601 cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt tacactaaat
      661 ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt ttttgatgga
      721 agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta atgtcggtta
      781 gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg gtcagcagca
      841 tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag aagcaggagc
      901 tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc gtgggcctgc
      961 cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac gaaaaagggg
     1021 agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc tggactttta
     1081 ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa gattttgtct
     1141 acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg tttgccgcgc
     1201 tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct gatcagcttg
     1261 gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg ttgccgtggt
     1321 ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac gtagaaagcg
     1381 gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt cttaaagagc
     1441 gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat aacgccaaaa
     1501 cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc actaagcgtt
     1561 acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat cagaagctgt
     1621 tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat tattatgcct
     1681 ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta agtatgacga
     1741 tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca gcgtggcatt
     1801 ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa caaatgagtc
     1861 aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat ggtccgcaaa
     1921 aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa attgcgattg
     1981 ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa aatcaggaat
     2041 ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc gcctcatggg
     2101 tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg cattcaggaa
     2161 atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca tcgacggaaa
     2221 ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc atggagcaag
     2281 caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg tggctgaaag
     2341 gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat attgtgaagc
     2401 attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt ctgatttagc
     2461 gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg aaggcaaagt
     2521 ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc tgtgcagcaa
     2581 gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc aatgacgagt
     2641 cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc gggtgatttt
     2701 tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc tcataacgta
     2761 aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct acgctgatcg
     2821 acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta attttggtaa
     2881 ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa cggatccagt
     2941 cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa ttactcagaa
     3001 tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc agcaagccag
     3061 aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata atggtgattt
     3121 tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta atctgctgtt
     3181 tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca atcagggacg
     3241 tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt tctataacat
     3301 aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat tgcaccgcat
     3361 ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc atcagaatca
     3421 atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta aacagttcag
     3481 cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa cccctggcgg
//
LOCUS       DAJVRP010000204         3474 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVRP010000204 DAJVRP010000000
VERSION     DAJVRP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.209"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(1..515)
                     /locus_tag="OQN12_004030"
                     /pseudo
     CDS             complement(1..515)
                     /locus_tag="OQN12_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(719..925)
                     /locus_tag="OQN12_004031"
     CDS             complement(719..925)
                     /locus_tag="OQN12_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCS1656162.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1032..1595)
                     /locus_tag="OQN12_004032"
     CDS             complement(1032..1595)
                     /locus_tag="OQN12_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCS1656163.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(2970..>3474)
                     /locus_tag="OQN12_004033"
     CDS             complement(2970..>3474)
                     /locus_tag="OQN12_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656164.1"
                     /translation="PLIDPVYKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPP
                     YITGLLDDVEKVLDKINILIDKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSK
                     KYTLLQCVEKLGVLVHYVKSPVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIR
                     NNPPELS"
BASE COUNT         1114 a          766 c          624 g          970 t
ORIGIN      
        1 gagcttcgtc aaataatcca gacgtaccaa taagcgctgg tactgctctg ggaaattagg
       61 atcgttaata tccaggatgt aacgtccatt agcagggaaa tgaactaatc cctggtgatc
      121 ttccagttcc aaccctaagg cactgtacca ggctccggta attaaccctc tcagggtgtc
      181 agagcgctca tagtttccca gatggtaata ggcgtctttg ttaaaaaaag gcagacgtga
      241 taatgacatt taaaggactg cgtatattct ttcgcccaga taatacaaac cgacgttgcg
      301 taggtgcgtt tgccttgttg ttttttacgg tgttgatctg cattaatttt cgcattcagc
      361 gagttacgga aacgagatat ctctccgggg atcaggttag ggaaacaggt aatgatatcc
      421 ccgttgtcaa tcacaggagg gtaatggaga tccccccgta ttgctgttat tctggaagtg
      481 tttttaatat tcgtattgat agttctcaga gatatatcac actctagata ttttaattaa
      541 gtatcagttc gatatcggca tattgtacac tacctcaggt agatgatgag atcagataca
      601 gggatcaact gacggtgagc gcagtcccca cattttatcc agggcttact gcatattccc
      661 aacagccatt cgttagccag gtagacgaat accctgattt gccgctggtt ttgctttcct
      721 atcgaagggc ccatacatca tcacgcccac gaaacaaccg acgaaataac gcaactttct
      781 catccgtgga taatacgaaa acgcactgca cagggctctg cggtttacgt cgccattcaa
      841 tcccatgagc ttcaagtaat gagaccagcc gggtattttc tgcccgcagt actgctagtt
      901 catcgctgtc agatttataa accatatgcg ttatcacttg tacctgtctc ccggcggcac
      961 cacgactgaa attcgtgtga aggcaaatcc acgcgataac ttacgatagt tcggacataa
     1021 gcccccataa ttcagttatg tcgtggtcga ccccggttac ttttttcggg aagcctgcta
     1081 cggcccagaa taataccgtg ttctttctga tcctgtttag ccagatagct aagggcgtaa
     1141 cgtaagtcgt ctaccgcaga tttatcacta tagtgaatca cgtgatcgat acgcaccggg
     1201 tatttgtctt tagcccggca cagatgaaaa taaccctccc cttccgtgat ccgcctccag
     1261 atatctccca gttgcctgga tatctgataa gagttcttgt gacgttgtcc atccaggtag
     1321 ccgataaaat gaatatgaag ccctttgtct ggtgtatatt ccattaccca gtaatatccc
     1381 gccaacatcg tctgggtttc gctaagtaaa cggtatattt ccatacacat actgtgctta
     1441 caggaatgcc cgaaactggg cgtgtctttc ctgtaagcaa aatcaattct gaaaggtaac
     1501 agtttagaaa aacgttgaaa cataccatcc atgtgttcat tcacgtcttt cagaatcatg
     1561 aaatccattt tgtaattggg attggcatta tacattttat agtccttact ttataaaact
     1621 cacaggaaga gagaattacg taaatactga gagtagagaa acgccgtacc acggcattaa
     1681 taaacgaagt aaaccagcag cacagacaac ctgataaaac tgttttgtat taccgcatta
     1741 ctgtgatgag gtaatacttc agaatacagt gagactctct taaactgaga accagtacga
     1801 attacaatgg aaaaacacac ctcatagaat tgagtttcag gaagcaccac taaattcttt
     1861 acaggaagta atctgacatt cacaaaactc agtatcggct agctacaggc aacatcgcta
     1921 ttaatattgt cgaaccatta agtaaagccg agagagacca cgggtattta atcattagca
     1981 taaaagaata atggtataca gaacataccc acacaccata agaagattgt tccatacaga
     2041 aaagatcata tttacctata cctataaact gtaacggagt cataacacgc tctaatagtg
     2101 ttccgatacg taaccagtgt taagtacgta caggacaaca ttacacacat aattcaccat
     2161 acagcttccc ccatatgctt cactgcgtag tgtatggaga tatatgcatt acacacatac
     2221 agaatactta gcataaagag gagtgacatt accacgtaaa gagataagtt atacaacaaa
     2281 catacaaaca atacggcaca gacacacaat accataactc caactaataa tactaattaa
     2341 ccttaattat ttatggtata tgaaataaat gtgattctcc cggaactaac taacctaacc
     2401 agacagatga tcactgtata aaaacttcag ttaatactca tttgttttgt cgactcagta
     2461 tataatgagt gaccagtaat caggataatc acacttacat aacctgtaaa aaagaacaag
     2521 aagaataagc cactaacata atgcaaaatg gcatctgaat cgatcgcagt ttggcattcg
     2581 aatcgatcat gatttggccc aatccgtgat cgaatagagc ggaatacgca acataattca
     2641 ccagacaaga gcttacaccg aattacagat tatgtattaa tgtcaaaact ggcatatttt
     2701 acagatacaa tgaacatgca acccatcaat ctaatagggg cggtatgtac atctgcccat
     2761 gaatgggatg gcgctaagaa tacaaaacaa ttagacatag atcacaaaaa cacaccattc
     2821 taattgaaaa acaaaacaaa caaataaatc atactattat agaattttct cttgccagac
     2881 atagataata cccctgctaa ttttgctaat ggttagcaaa attagcaggg tatataaaga
     2941 ttattgaccc aaaacacact cagatgaatt cacgacagct caggagggtt attcctgatg
     3001 tcatcttcac aaataatggt taacgcatca tatatcattc tgcgtctgtt cctgttttcg
     3061 gccttatcgc catacaacat catcacattt ttaatttcat cgaccggtga tttcacatag
     3121 tgaactaata caccaagctt ctcaacacat tgcagtaaag tatatttctt actgcctgtg
     3181 aaaagcccta tcatagtttc tttacgttcg cgactggata atactttgtt ttccataatg
     3241 acttctgcca gcgtcttacc atcataaaca tactctttat ctatcagaat atttatctta
     3301 tccagcacct tctctacgtc atcaagcaaa cctgtaatat atgggggcgc aatatatggt
     3361 ttcgtactga tcttttcaag catacaatta tacatcttaa taacacgcat cagggtaaaa
     3421 ttgttcgcat catactgtat ttcaccctcc ttatatacag gatcaataag tggt
//
LOCUS       DAJVRP010000205         3335 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVRP010000205 DAJVRP010000000
VERSION     DAJVRP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.210"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(144..347)
                     /locus_tag="OQN12_004034"
     CDS             complement(144..347)
                     /locus_tag="OQN12_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656165.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(349..504)
                     /locus_tag="OQN12_004035"
     CDS             complement(349..504)
                     /locus_tag="OQN12_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCS1656166.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(707..1114)
                     /locus_tag="OQN12_004036"
     CDS             complement(707..1114)
                     /locus_tag="OQN12_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1656167.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1191..1418
                     /locus_tag="OQN12_004037"
     CDS             1191..1418
                     /locus_tag="OQN12_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1656168.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1402..1824
                     /locus_tag="OQN12_004038"
     CDS             1402..1824
                     /locus_tag="OQN12_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656169.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1902..2691
                     /locus_tag="OQN12_004039"
                     /pseudo
     CDS             1902..2691
                     /locus_tag="OQN12_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2698..3335
                     /locus_tag="OQN12_004040"
                     /pseudo
     CDS             2698..3335
                     /locus_tag="OQN12_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          860 a          733 c          901 g          841 t
ORIGIN      
        1 atcaactccc ggacggaact tatgtttccg aaaagggcgg tgatatttat caaattcgca
       61 gccagatatc tgactatgca gaccgactat ccgggtatcg cgcgtctgtt tttgtttgcg
      121 aattcagtca atgcgcatgg tatttatacc ccgccaagac ccggtgaata tcctgcacgg
      181 ccttctgctt tgtagaaggc ttcttcgcta tcataatcgc cagattccag cgcttcttta
      241 gccagccaga tgcgtgcccc agtgcctgca tttggctcca gttgctggag gcgttttgca
      301 tcttctagga gtagagcgat aacgtgtttt aattctgtct cgttcatttt actcacctga
      361 atgtcttccc aaccaacgac gtgcgccagc ttcggtttta aacgttttgc ttttggtata
      421 cgttatggcg gtgaatgtgc cgtcctggtt ggggaacacg ccgtacaccg gagattcgtt
      481 gttgccaaga tcgatagtat tcatgttgac cccatttccc cttaacgccg gggtagcgga
      541 actgtttgct gagaacaccg tgcggtgtct tgatgaataa aatttagaat aacctaagga
      601 gggtgatcaa gatttttatg tagaaaaacc taagtttttt gatgtaaaaa acacaagtgt
      661 ttgaaagttt gtgcttttta ttacagggtg tggagaaaaa aggggattat ttgtttgcgc
      721 ttcttttgcg agctttgagt agttcttcaa aaagtttgtt gaaattttca actcgagcac
      781 gcatctctga caacagggcc ttttgctctg actcaggcag tgcgtcgaac agttgaagta
      841 actctttttg atcttctgtc agaatggctg gctgattatc cgggatcggt tcgcctggtt
      901 gtttatcttc atccccaaaa agaagccaag tcggtgagca ctgaagcgct tggctcagtg
      961 cgaataatct tttccccgcc ggctgtgttt catctctttc ccattgagaa attgttacgt
     1021 gagccacttt gaccagctta cctaatgcgg cctgagacag ttttaatttt ttacgcctat
     1081 gtaagaggcg agcaccgaag gtttcgtttt tcatattagg gaattctaat ttttcttgac
     1141 ttaggtttct ctacggcctg gtttccttag gaaaatctaa ggagctcgat atgttgaaaa
     1201 ttgatgctat agcgtttttt ggcagcaaaa caaagcttgc caatgccgca ggagttaggt
     1261 tggcaagcat tgctgcatgg ggggaactgg ttcctgaagg tcgcgcgatg cgcctgcaag
     1321 aggcatctgg cggggaactt cagtacgacc ccaaagttta tgacgaatat cgtaaggcaa
     1381 aacgacctgg gaaggtgatt catgaaaatc aggcatgagc acatcgaatc agtgctgtta
     1441 gccttggcag ccgaaaaagg gcaggcgtgg gtcgctaacg caattactga agaatatctg
     1501 cggcaggggg gcggcgaatt gtctctggta ccaggcaagg actggaacaa tcagcagaat
     1561 atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa gggaaaaaat tcagaaactg
     1621 atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc accgactctg tatcttcgat
     1681 accctggaac gccgtgcatt actggcggcg caggaagcgt tgagtacggc aattgatgcg
     1741 catgatgatg ctgtccaggc cgtttaccgg aaagcacatt tcagcggtgg tgggtcgccc
     1801 ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc cggattgttt tgttcggtat
     1861 cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca aatggcttca aactggataa
     1921 agctcgaggt tattacgccg gataagccgg aaatattcag gcttgctgag attctgaata
     1981 ttgatccaga tgccgcatta gggaaggtta ttcgcttctg ggcatgggcg gatcaacaaa
     2041 tgatagacgg taatgcagat tgtaacgctc gcggcgttac aaaaagtgca atagatcgca
     2101 tcacttttat gtctggtttt gctgatgcgt taattcaggt tggatggctg gtcgaaaatg
     2161 acggtgggct ttctctacct aactttgaac gtcataacgg aaaaagctct aaaaaacggg
     2221 cggttacaaa cgagcgagta acaaaaatac gcgaactgaa acgaaaaggt aacgctgcca
     2281 gcgttacaca aacggatcaa aaagcgttac cagtggaaga ggaagaggaa gaggaagagg
     2341 aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg tctaaaaaat
     2401 tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg catgagtggg
     2461 tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat acgggaactg
     2521 gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag catcgccaat
     2581 gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct ccgacaggtt
     2641 aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta acggaccatg
     2701 aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc gcaacatgta
     2761 accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga aggccagaaa
     2821 cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat tctgaatcgc
     2881 tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt gcagaacgac
     2941 ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat gaccgggtgt
     3001 acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc ggcagctatc
     3061 gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc tgatgttatg
     3121 agcgccctgc acgccagcta tgacgatggg cagtcaggcg aaaaattttt gcgggagctg
     3181 tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac gaaaaacgag
     3241 caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag cgtggggatg
     3301 ctgacaaacc tgaactatga ggtgatgaaa acatt
//
LOCUS       DAJVRP010000206         3267 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVRP010000206 DAJVRP010000000
VERSION     DAJVRP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.211"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..203
                     /locus_tag="OQN12_004041"
     CDS             <1..203
                     /locus_tag="OQN12_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656170.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="OQN12_004042"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="OQN12_004042"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS1656171.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="OQN12_004043"
     CDS             2102..2818
                     /locus_tag="OQN12_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1656172.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          922 a          706 c          705 g          934 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaact
//
LOCUS       DAJVRP010000207         3193 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVRP010000207 DAJVRP010000000
VERSION     DAJVRP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.214"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            102..3005
                     /locus_tag="OQN12_004044"
     rRNA            102..3005
                     /locus_tag="OQN12_004044"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3193
                     /gene="rrf"
                     /locus_tag="OQN12_004045"
     rRNA            3101..>3193
                     /gene="rrf"
                     /locus_tag="OQN12_004045"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          849 a          700 c          988 g          656 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttattacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3181 ggggtctccc cat
//
LOCUS       DAJVRP010000208         3177 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVRP010000208 DAJVRP010000000
VERSION     DAJVRP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.215"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            847..1041
                     /locus_tag="OQN12_004046"
                     /pseudo
     CDS             847..1041
                     /locus_tag="OQN12_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
     gene            complement(2066..3175)
                     /gene="fepE"
                     /locus_tag="OQN12_004047"
     CDS             complement(2066..3175)
                     /gene="fepE"
                     /locus_tag="OQN12_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1656173.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          945 a          775 c          658 g          799 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg gcgtcctgta
      361 aaaaaacaca ccgcgccgct agcagcaccc ctaatataag atgatgtttt tttatgaaaa
      421 tagtcagtac cacccctaaa aagcggtgtt ggcgcagcta caacaccgcg ccgacaccgc
      481 ttttttaaat atcacaaaga gagcaagaag aactaatttg tcataactct ctatttataa
      541 agaaaaatca gcaaaaactt gtttttgcgt ggggtgtggg catttcaggc atgagaacag
      601 actcgcgttt ttccggagta tcagcggaat tattgattaa gaaacactcg acgcagagcg
      661 aaattttatg caacagcggc tgcactgcac aggaattatt tatcagttga tatgctgtgc
      721 catgatacgt cctaaagaaa caggacagga tgtgcaattg taataccatc gcccgcaacg
      781 tcttacaatt accacctgct cagggtcccc cccccgacac ccggaactga cggagaacaa
      841 cggaaggtga acaaataaca acaaactgcg ctgaatatgg cgcgatgtat caaaagccag
      901 agcctgacac tgcttgaaaa actggatgct ctggatgccg acgaacaggc ggccatgtgc
      961 aaacgactgc acgaactcgc agaagaactc cagaacagca tccagattcg ctttgaggcc
     1021 gaaagtgaaa caggaacata acgaagctga cggaggcggt cacggctttc ccgtgggcgt
     1081 tttcgtcagc agacaagccc gtcagggcgc gtcagggggt tttagcgggt gtcggggcgc
     1141 agccatgacc cagtcacgta gcgataacgg agtgtatact ggcttaatta tgaggcggca
     1201 gagcagatgg tgagagaaca gcgacatatg aggtgtgaaa tgctgcacag atacgtaagg
     1261 agaaaatgca gcttccggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1321 gttcggctgc ggcgagcggt gtctgctcac gcaaagacgg atatacggtt atccacagaa
     1381 tcaggggata acaccagaaa aaacatgtga gcaaaagaga ggccccgaaa aaaggccgcg
     1441 ccggtggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1501 agtcagaggt ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtggct
     1561 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     1621 cgcgttcgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     1681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1741 cttgagtcca acccggtaag acacgactta acgccattgg cagcggccac tggtaaacag
     1801 gatgtgcgag agatgtattg agagttcttg aagtggcagc cttactgagg ctacactaga
     1861 aggacagatt tggtgacatg tctcgcacaa gactgttacc acggttcaga agttccccaa
     1921 cttactgaac cttcgaaaaa ccacctcccc aggtggtttt ttcgttttct agagtagaag
     1981 actacggctg taagggaatg atctcaagaa gatcctttga actttttata aaagttgctt
     2041 ataaaatgat gcatcagcac aacttttact caatcacact catctttctg gcctccatgg
     2101 catgacgtaa aagtatatag ccacatgcaa gcatccctcc aatgacagaa gaaagaatta
     2161 caattatggc attacttggc ccatcctttc tgaccggtaa cgttgggctc aattgatact
     2221 gaaatggcat aaaatcaaca tcccccactt tcgccacaac caattgattc acaacatatt
     2281 gacgatttcg caactcccca ctcaattccg aaacatcctt gattgatttt ttgatgttca
     2341 atttagttgc tataccatca gaaccgagag ccacaggaaa atctgggtca tccttcataa
     2401 tctgaccatt gctgtataca ggttttttta ttccagccgc attagcaacc tccagtgaat
     2461 aattgaggcg tcgaatatct gcattcagct ggtttctaat ttttatctca tccagtgcaa
     2521 gaatttcttt ttcaacatta gttttaactt ctagtttatt tcttatatct tccatcaaat
     2581 cccttacaac aagggaagaa acataattaa tatacccaga caacacatcg tgagcttctt
     2641 tacttgttgg agcagtaaaa ctcagagtcc atgatacata caatgatgac ttgtcgtttt
     2701 tcttactggc attatcatca atcgacttca tatttttact caactcaaca attgcacgat
     2761 gcagttcgag agcgctaatg ttattctttt taataatctc cataacataa ggagatgatc
     2821 tcatatactc attcaacgaa ttaacggact gaaatttctt aataaacaga gcaaatgcgc
     2881 tatcacgatt gatatcaaaa cccactccca gaacatgaag cttggaaagc tccttattta
     2941 attcactcca ttgaacagac tcagcaggag taaccacagc agaactagtc catttttgtg
     3001 gcaaaacaaa agaaacaaga aagccaatac aagcaaaaaa tagaatcacc ccaacaattt
     3061 ttatttttgc tgcccataaa acgctaatca agccaaaaag gtcaatttct tcattaccat
     3121 tcggcaacat gttgtaatta gaaaaatgtt cgtcagagac ctttgagatt ttcattg
//
LOCUS       DAJVRP010000209         3087 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVRP010000209 DAJVRP010000000
VERSION     DAJVRP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.216"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..357)
                     /locus_tag="OQN12_004048"
     CDS             complement(<1..357)
                     /locus_tag="OQN12_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1656174.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFVYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLK"
     gene            complement(396..683)
                     /locus_tag="OQN12_004049"
     CDS             complement(396..683)
                     /locus_tag="OQN12_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656175.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(933..1088)
                     /gene="hokD"
                     /locus_tag="OQN12_004050"
     CDS             complement(933..1088)
                     /gene="hokD"
                     /locus_tag="OQN12_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1656176.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1316..1981)
                     /locus_tag="OQN12_004051"
     CDS             complement(1316..1981)
                     /locus_tag="OQN12_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1656177.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2150..2506)
                     /locus_tag="OQN12_004052"
     CDS             complement(2150..2506)
                     /locus_tag="OQN12_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656178.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(2564..2986)
                     /locus_tag="OQN12_004053"
     CDS             complement(2564..2986)
                     /locus_tag="OQN12_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1656179.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            complement(3001..>3087)
                     /locus_tag="OQN12_004054"
     CDS             complement(3001..>3087)
                     /locus_tag="OQN12_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1656180.1"
                     /translation="RMVMNGGRWVNFNWESWRPNVSHSRVVK"
BASE COUNT          767 a          816 c          672 g          832 t
ORIGIN      
        1 ctttagcaat gccgtatcac gggttctgcg tgtcgcaact gtattcagat attgctctgc
       61 cgcttcgctc agagctggcg tatgttcccg gcctactgat tcacacaggt aatcaacgaa
      121 accggacacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag tccagtaaac
      181 gaaacccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac gcacacgctt
      241 aaagtctgcg tgtatccact cacctatttt gatttgatgc agaaaatcgc aactctccgg
      301 cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat gtgccatcgg
      361 ctttctccgg tggcacggtg ttacacagca ggagttcaat cctgctcaag attgtagatg
      421 agtttattct tcagcaaaag cagaaaacca gccttaaaac caatcttttt caaaacccgt
      481 aatgatgtga caaattcgtc ctcacgcaaa ataaaaccgt ccgtcagaag tccattacaa
      541 aaataaaata acacagcacc gctcttcctt tgttgagatt gcaaacatct aatgcggcaa
      601 tggctgacaa tcgctccatt ctcaacgcgc acagcataga ggccattttc actaaaaatt
      661 tcacgcaatt cttcgatttt catcttcaga atccttccag ataaatagct ctcccctgtt
      721 cggggtccat ccctcttctc cctgcgcgct acttaagtga gtcgattcta tctgcgaagg
      781 tgcgcgaatc aaattcaccg gaaataaaca acaaaaaacc cgccgaaacg ctgagagatc
      841 acagcgatgg gcgggttaag tgcgggtgcg ttgaggatgc ctgacacatc agaggtgggc
      901 ggggatggga tcagctcccc acccggtcac tcttacttcc tggattcgta gtctacgaag
      961 acagcgacct ccgtctggcc ggttcggatt cgtacctcac agaggtcttt cctcgttacc
     1021 agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat cgccttttgc
     1081 tgcttcatag cctgcttctc cttgcctttc ggcatgtaag aggctaacct acatgtgcaa
     1141 agcatgaaat tggcctcaga ttaatgttaa gcgtcttgcc ggacgcgtaa tgttaactgg
     1201 ggcttttctc tatctgcctt ttggtgttca tgcctgaagc agatagcctc aagcacccgc
     1261 agtcattcta cttacctcgc caatatgaaa tcaatcagaa aggtgcccca taaaatcact
     1321 ccttctcttc tttaccgtag tggagttgac caattttgat aagagggcgt ccctgagatt
     1381 tgcggtgtag attggtatcg cgcagagaat acacacagcc acaatattcc tgctgataga
     1441 atttttcgcg cttgctgatt tcaatcatac gggacgagcc gccctgcttg cgccagttat
     1501 aatcccagta caccatgccc ggataatgcg cgacggctcg ccgcccacag tcgttaacct
     1561 gctgcatatt tttccagcgt gaaatgccca gtgaactgct gatcacactg aaaccatttt
     1621 cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa acacatggtg caacggatcc
     1681 ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa ccagttgtct gtgtcgtaat
     1741 cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg aatattctca tccttacgaa
     1801 ttaaatactc tttctgagga tgaatgttcg ggttgtagaa aaagatggtg tagtcgattc
     1861 ccgaggcctg aagcgcctcc atcacctcac cggaacatgg agcacagcaa gagtgcagta
     1921 gtagtttgtt tgccccgttt gggagctcca atttgggccg tttgaaatca gcaactgtca
     1981 taaatatgtt tattgaggtc ataaaaatat caaagagtgt agcattagaa cggggctatc
     2041 ggaaacagat gtgtgactgc tccccgccct ttcgggcggt ctcctgatga tttgagggtg
     2101 cagaaatccc tccggttaag gattaaattt ttaacagtgc taaatttaat tattcagttc
     2161 tggattttgt cgccctgcgt atccgcgctt tcgcgttacg ctcaatctgt atcagctttt
     2221 ctatatttct ccgcctttcc tgttcctcct ggcgcaatag ccttacatca tctgccagtc
     2281 tggtttctct ttttgccaca gagagcatcc agtcaaacgg ctccacaacc gcaccgcaga
     2341 ttttacagcg gacctgacgc tctttttcgt caacccgtac agaggcgtga tggcaatatg
     2401 gtctttccga tggcttataa agaaaattaa cctgattacg agggtcatcc tcttttatcg
     2461 gaaataaaac gatattgctt aactcatctt ctggttttat ttccatgctc ctctcctttg
     2521 atgcgaatgc cagcggtaat tgaagcctga tagctaattt cattcacagt atcgcctcct
     2581 gaaaattacc ctgatagaaa gccagtacac gctgcatagc ttcgctcttc cggcactcgc
     2641 gacagattat attcaggcgc ctgtcgtagc ggcgtatttc tccgtcaggt aatgaccaga
     2701 taaggtccgg atcaacaaca accggtttcc tcacctttac tctcgagagt tttttgcggg
     2761 cgctttgcca gtccttgcga gcctgttcag acgggaataa gccgtagcca gaattatata
     2821 catcaccact tgcgaccagt tctatgcata agcgaccgac agacgcacga ctgatacctg
     2881 tttcatcgga caactgccga atcgtgaccc gaccgtcctg atgcacgagt tccacaattc
     2941 gcgtcttcag ttcttcccgc tgttcgggag taaaaggttt cgccataaat cctcctgaaa
     3001 ctacttaaca accctcgaat ggctaacatt cggacgccag ctctcccagt taaaattaac
     3061 ccagcgcccg ccgttcatga ccatgcg
//
LOCUS       DAJVRP010000210         3056 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVRP010000210 DAJVRP010000000
VERSION     DAJVRP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.217"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            532..1698
                     /gene="nhaA"
                     /locus_tag="OQN12_004055"
     CDS             532..1698
                     /gene="nhaA"
                     /locus_tag="OQN12_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1656181.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1764..2663
                     /gene="nhaR"
                     /locus_tag="OQN12_004056"
     CDS             1764..2663
                     /gene="nhaR"
                     /locus_tag="OQN12_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1656182.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAAQRICNTDYSALFSPAVR"
     gene            complement(2701..>3056)
                     /locus_tag="OQN12_004057"
     CDS             complement(2701..>3056)
                     /locus_tag="OQN12_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCS1656183.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          719 a          678 c          750 g          909 t
ORIGIN      
        1 catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc tatctgccgt
       61 tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc gctctcttta
      121 acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt ggcgtaattt
      181 tttaatgatt taattattta actttaattt atctcttcat cgcaattatt gacgacaagc
      241 tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa attaattatt
      301 acttttccta attaatccct caggaatcct caccttaagc tatgattatc tgggcttagg
      361 gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg cgcaaattct
      421 tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa cgatgattcg
      481 tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa agtgaaacat
      541 ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat tgccgctatc
      601 ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga ctttctggag
      661 acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct gttatggata
      721 aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa acgtgaactg
      781 atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc cgctattggt
      841 gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc gattacccgc
      901 gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt actggcgctg
      961 ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc tattatcgac
     1021 gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc gatggcctct
     1081 cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg tggtgtacgc
     1141 cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt gaaatcgggg
     1201 gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa agagaagcat
     1261 gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc gtatctgatt
     1321 ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac gctggatggc
     1381 ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa accgctgggg
     1441 attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc tgagggaacg
     1501 acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac tatgtctatc
     1561 tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg ggcgaaactc
     1621 ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt acgcgttcgt
     1681 ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct cccttttcat
     1741 tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg tattacttct
     1801 ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat ttaactccac
     1861 aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa ttatttaaac
     1921 gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc tatgccgata
     1981 aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa gaatccaatt
     2041 tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt agcgtactta
     2101 acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc acccacgaaa
     2161 tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac tgtccgatag
     2221 actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc gtgagtttct
     2281 ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa cggcgacttt
     2341 tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt aactcccagg
     2401 gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa gcttttggtg
     2461 cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt tatgccgata
     2521 aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat gctatttttg
     2581 ctgagcggat gattcagcac ccggcggcac agcgaatctg caatacggat tattctgcgc
     2641 tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg gggagataga
     2701 ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg agacattgtt
     2761 tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat ggggatgctg
     2821 aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat tggagagttg
     2881 aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta atatgctggt
     2941 tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg gttcaaaaac
     3001 taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact gataat
//
LOCUS       DAJVRP010000211         2995 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVRP010000211 DAJVRP010000000
VERSION     DAJVRP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.218"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            173..1276
                     /gene="proB"
                     /locus_tag="OQN12_004058"
     CDS             173..1276
                     /gene="proB"
                     /locus_tag="OQN12_004058"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1656184.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1288..2541
                     /gene="proA"
                     /locus_tag="OQN12_004059"
     CDS             1288..2541
                     /gene="proA"
                     /locus_tag="OQN12_004059"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1656185.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2656..2731
                     /locus_tag="OQN12_004060"
     tRNA            2656..2731
                     /locus_tag="OQN12_004060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2689..2691,aa:Thr,seq:cgt)
     gene            complement(2746..>2995)
                     /locus_tag="OQN12_004061"
     CDS             complement(2746..>2995)
                     /locus_tag="OQN12_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1656186.1"
                     /translation="AGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRY
                     AHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          721 a          753 c          838 g          683 t
ORIGIN      
        1 aaatgtggta atttattaaa tctgtaataa aagcgtaaac aactgccgct acgcttgctg
       61 atcccgcgca acaaaacgcc atgctttgct cgcagatggt tggcaaccga cgacagtcct
      121 gctaaaacgt tcgtttgata tcatttttcc taaaattgaa tggcagagaa tcatgagtga
      181 cagccagacg ctggtggtaa aactcggcac cagtgtgcta acaggcggat cgcgccgtct
      241 gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg cagttacatg ccgccgggca
      301 tcggattgtt attgtgacgt cgggcgcgat cgccgccgga cgtgagcacc tgggttaccc
      361 ggaactgcca gcgactatcg cctcgaaaca actgctggcg gcggtagggc agagtcgact
      421 gattcaactg tgggaacagc tgttttcgat ttatggcatt cacgtcgggc aaatgctgct
      481 gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc
      541 gttgctcgat aacaatatcg ttccggtaat caatgagaac gatgctgtcg ctacggcaga
      601 gattaaggtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg cgggtgccga
      661 taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgatccgc gcagaaatcc
      721 gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg cgattgccgg
      781 tgacagcgtt tcaggcctcg gaactggcgg catgagtact aaattgcagg ccgctgacgt
      841 ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg gcgttattgg
      901 tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga ctccgcttga
      961 aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg
     1021 ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt
     1081 gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag gtcgcgatat
     1141 cgcccacggt gtcagtcgtt acaacagcga tgcattacgc cgtattgccg gacaccactc
     1201 gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc accgtgatga
     1261 catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc cgcgaagcaa
     1321 gcctcgtata aattagcgca actctccagc cgcgaaaaaa atcgcgtgct ggaaaaaatc
     1381 gccgatgaac tggaagcaca aagcgaaatc atcctcaacg ctaacgccca ggatgttgct
     1441 gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc gtctggcact gacgcccgca
     1501 cggctgaaag gcattgccga cgatgtacgt caggtgtgca acctcgccga tccggtgggg
     1561 caggtaatcg atggcggcgt actggacagc ggcctgcgtc ttgagcgtcg tcgcgtaccg
     1621 ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg tgacggttga tgtcgcttcg
     1681 ctgtgcctga aaaccggtaa tgcggtgatc ctgcgtggtg gtaaagaaac ctgtcgcact
     1741 aacgcggcaa cggtggcggt gattcaggac gccctgaaat cctgtggctt accggcgggt
     1801 gccgtgcagg cgattgataa tcctgaccgt gcgctggtca gtgaaatgct gcgtatggat
     1861 aaatacatcg acatgctgat cccgcgtggt ggcgctggtt tgcataaact gtgccgtgaa
     1921 cagtcgacaa tcccggtgat cacaggtggt ataggcgtat gccatattta cgttgatgaa
     1981 agtgcagaga tcgctgaagc attaaaagtg atcgtcaacg cgaaaactca gcgtccgagc
     2041 acatgtaata cggttgaaac gttgctggtg aataaaaaca tcgccgatag cttcctgccc
     2101 gcattaagca aacaaatggc ggaaagcggc gtgacattac acgcagatgc agctgcgctg
     2161 acacagttgc aggcaggccc tgcgaaggtg gtggcggtta aagccgaaga gtatgacgat
     2221 gagtttctgt cattagattt gaacgtcaaa atcgtcagcg atcttgacga tgccatcgcc
     2281 catattcgtg aacacggcac acaacactcc gatgcgatcc tgacccgcga tatgcgcaac
     2341 gcccagcgtt ttgttaacga agtggattcg tccgctgttt acgttaacgc ctctacgcgt
     2401 tttaccgacg gcggccagtt tggtctgggt gcggaagtgg cggtaagcac acaaaaactc
     2461 cacgcgcgtg gcccaatggg gctggaagca ctgaccactt acaagtggat cggcattggt
     2521 gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa aagtagccat ttgattcaca
     2581 aggccattga cgcatcgccc ggttagtttt aaccttgtcc accgtgattc acgttcgtga
     2641 acatgtcctt tcagggccga tatagctcag ttggtagagc agcgcattcg taatgcgaag
     2701 gtcgtaggtt cgactcctat tatcggcacc attaaaatca ataagttaca catcattagt
     2761 accttcctta ttttttgact gggacaaatt tgggaccgat gggttcagga tcgagtctat
     2821 ttgccgtgcg tgttcggtaa ggtgattagg tgcaaggtga gcatatcgac gaaccatttc
     2881 gatagactcc cagcctccca tttcctgtaa cactgacaac gggactccgg cttgaaccag
     2941 ccaacttgcc caggtgtgtc tcaagtcgtg aaatctgaaa tcatcaatac cagcc
//
LOCUS       DAJVRP010000212         2926 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVRP010000212 DAJVRP010000000
VERSION     DAJVRP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.219"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="OQN12_004062"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="OQN12_004062"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCS1656187.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="OQN12_004063"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="OQN12_004063"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCS1656188.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1134..1892
                     /gene="tam"
                     /locus_tag="OQN12_004064"
     CDS             1134..1892
                     /gene="tam"
                     /locus_tag="OQN12_004064"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCS1656189.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1896..2810)
                     /locus_tag="OQN12_004065"
     CDS             complement(1896..2810)
                     /locus_tag="OQN12_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCS1656190.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          771 a          678 c          781 g          696 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact accggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg
     1141 actggaaccc ctctttatat ctgcactttt ccgctgaacg atcgcgtccg gcggtggagc
     1201 tgcttgccag agtgccgctg gagaatgtcg aatatgttgc cgatcttggc tgtggcccag
     1261 gtaacagcac cgccctgctg catcaacgtt ggcctgcggc caggataaca ggcatcgact
     1321 cgtctccggc gatgattgct gaagctcgca gtgctttgcc agactgccag ttcgtggaag
     1381 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     1441 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
     1501 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     1561 gcgaagttgc ttgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
     1621 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     1681 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     1741 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     1801 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     1861 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     1921 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     1981 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg ttcttccagt
     2041 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     2101 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     2161 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     2221 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct
     2281 gaaagctgtc cattgcggcg ctgaaccgcc aaccattctc ccattattaa caagatacgg
     2341 ttagctggag agttcgaagc cagtacacgc tgaagatctc cagtcttgag ataatgagcc
     2401 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     2461 tgcagcctgg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctctcgc
     2521 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     2581 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     2641 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaatataaca
     2701 gcaatagaaa agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagccg
     2761 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     2821 aggcgtggaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     2881 caaattacct agaaccacgg cagcctgacg gctgcaaatt gaaaac
//
LOCUS       DAJVRP010000213         2908 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVRP010000213 DAJVRP010000000
VERSION     DAJVRP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.220"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..921)
                     /locus_tag="OQN12_004066"
     CDS             complement(<1..921)
                     /locus_tag="OQN12_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCS1656191.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
     gene            complement(1048..2697)
                     /locus_tag="OQN12_004067"
     CDS             complement(1048..2697)
                     /locus_tag="OQN12_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1656192.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          656 a          779 c          771 g          702 t
ORIGIN      
        1 tcctccgggg aagcgcatgg cgtccgtcat cgacgtcagc agatggcaga cttttccttt
       61 gcgcgtcacg gtcagcaggc gggctgtcac ttcatttccc agtcccggcc acttttttcg
      121 tgcctgcggg ctggttttca gcttcaccag atgatcgcct ttacccagtt ttctgagctc
      181 ttcatattgc gctccctttc tgagaggtat catccagtgg cggtgttctc ccgccaggct
      241 ccagccattt aacagtccca gtgagtaata acctttatcc attaacgtca gagtgttatc
      301 gccggtttgt tctataagtt gctcagcaag ctcattttcg ctgttcttca tcgtgccgaa
      361 ggctgcagcc gtcagcagat ggctggtcag ttccatctgg cagaccattt tgacctgcgg
      421 gtagagcgcc gggttcccgg catgtgtctg gcgggggaag gctgcatcgt tctctggtgt
      481 atccggtgtg cgccagaaca caccatcgat ggccagcagg gtcaggccgc accagtgcgg
      541 atgcggcgtg gcgttatgcc agagctgcgc tgttttcgtg aacacgcggc ggacagcctc
      601 acttcccagg cgctggcggg cctgaataac ggcactgggg gcaacgaagg ggcgattgcc
      661 cggcagcatg atgtccaggc gattcacaat ctggtgaaga ggttctttac gctcaagcgc
      721 catgccaaca atacaccaga ccatcatttc gaggggaaga cggcgcttgc gtagcgttac
      781 agtacctgat tcggcaaggc aacgagagat gagttcgggg tcgaggtaat cccccagaga
      841 agtcagtggg ttacgcagag aatcgtaacg ggataccaga tcaagagcct gtccaatgtg
      901 cataaaaaaa tccggaaaca agtgagcgtt tccggattct tacacagcca ctggatcggt
      961 caactgatcc ttaactgatc ggcattactc ctcctgaaga ggataatttt acagccagca
     1021 ggaagttagc ggctggctgt aaaaatatta tggttcgccg tacaaaccaa ttaaacggcg
     1081 taccacaccg ttagtccagc caaacccatc ctgcaacgga tactcgccac cgccgccttc
     1141 gcggggaaca ccatcggcaa tatggtattt ctcgatcatt ttgtgctgtt ccagatagaa
     1201 ctgattcacc gttttcagcc agctgcgcgc gatttcatca cccagaaggt catcgccata
     1261 cattttaaat ccctgaattg ccatccattg taacggtgcc cagccgttgg gtttatccca
     1321 ctgttcaccg gtttcgtact cgcttgccaa aatcccgcca ggtgtcagta accggctgcg
     1381 tacggcgttt gccagacgat cggcctgttc atggttcgcc atgccgacat agagcggcac
     1441 aatggcggca gcggaaaaca gcgccagttg ttcgcgtcgc cagtcgtaat cgcggtagat
     1501 gccgttttca tcatcccaga ggtaacggtt taccgcatcg cgacgggcac tggccttctg
     1561 gcggaacagc gcttctgtct ctttctcgcc tttcagcgcc gagatgttgg cgatggcgct
     1621 ctccagttta aacaggaagg cattcagatc gatggggatg aactgggtgg tacgaatgct
     1681 cgccagacga ccagtatcac gcagccaacg ggaagagtaa tcccaaccgg aggccgctcc
     1741 cgcgcgtaaa tcgcggtaca cctcgttggg cgggcgacca gaatgtttcg cggtttcaac
     1801 gtcctcaagc caggattcgt cacgcggcgt gtcgcgatcg tcccagtaac ggttgagcag
     1861 cgatccgtcc ggcatccgca caacatggcg ataggcctga ttagggatca acgattctgc
     1921 accgtccatc cagaaggcat attccatttt aaggtggtcg agatagcggc gcgcaccgcg
     1981 tacaccatct tcttcaaaca actccaccgt cagcgcaaaa accggtggtt gcgagcggct
     2041 caaataatag gtgcggttgc cgttggggat gtgaccgtag ttttcgatca tccaggcgaa
     2101 gttatcggcc atgcatttca gcaaatcttc ccgaccactt tccgccagtc ccagcatggt
     2161 gaaataggaa tcccaatagt aggtttcgct aaaacggccg cccgggacaa tatatgactg
     2221 cggtagcgcc agcagagaag accacggaat gtgatcctgt ggttcgcggg ttagcaccgg
     2281 ccacagctgg tcgatatgct ctttcaggga attttgcggg tccgatacat actcgctgga
     2341 gtagacctcc ggcagccaga agtgattttc aacaaacttg cgcaagtcaa aatcacgatg
     2401 acggcgcact ttacggtagc ggattaaaat atccagcggg tccattttgg gtgcgcagtc
     2461 gggaaaggtt ttactgtcgg ggaaaatttt cgccgactga acatgctcga acagttcgag
     2521 atagcgatcg gcaggcgtca gcgcatcaga ggcgggcagc ccttcaatca tctcgggttc
     2581 cggttctgcc tcgatcatct catccagttt taattcatac gggtccagtt catagcagag
     2641 atcgacctcg atcatcagtt cgtctggatt agggttttga attttctgat tgagcataag
     2701 gatccaacct ccgaaattcg tcgctaatta tcgggctttt ctttaagggt agtcattcgc
     2761 tccgcctggt gggcgacaaa gcgtgcggtg gatcacgttt attaacttgt caaaaaatat
     2821 aacgcactaa tatcaatgga atttataaaa atacggattt ggtgttgttt ttcattttca
     2881 gaacattcaa tcctgctctc gccgcaat
//
LOCUS       DAJVRP010000214         2783 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVRP010000214 DAJVRP010000000
VERSION     DAJVRP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.221"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            87..1001
                     /locus_tag="OQN12_004068"
     CDS             87..1001
                     /locus_tag="OQN12_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1656193.1"
                     /translation="MQKNQNGADIPGKDTFTKNIGACRAFHSSISTGAGNWTTAQLIE
                     WLDSQGAFNHPYWMCKCSWSYANNKIITDTGCGTIHLAGCVIEVMGNKGAMTIRVTTP
                     STSTGGGTTNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSTVGNDKAVGWNS
                     ISGVYNANIGGATTLILHFYMGAGSCPAVQFRVNYKNGGIYYRSARDGYGFEADWSEF
                     YTTTRKPSAGDVGAYTKAECDSRFITGIRLGTKSSVQTWNGPGWNDKSGYVVTASINS
                     NKDELIDTTQARPVQYCINGTWYNAGSI"
     gene            1004..1537
                     /locus_tag="OQN12_004069"
     CDS             1004..1537
                     /locus_tag="OQN12_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1656194.1"
                     /translation="MQHLKNIRSGNPKTVKQYELTRQFGVVWLYSEDGKNWYEEQKNF
                     QDDTIKIAYTSDGVIVAINKDVSTINPEGLSVVEVADITANRRADISGKWMFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTAWMLYAQKV
                     ESTDTSILPVTFPEQPE"
     gene            complement(1565..2092)
                     /locus_tag="OQN12_004070"
     CDS             complement(1565..2092)
                     /locus_tag="OQN12_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1656195.1"
                     /translation="MMHLKNIKAGNAKTLEQYKLTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVSITKDASTLNPEGFSVVEVPDITANRRADESGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(2094..>2783)
                     /locus_tag="OQN12_004071"
     CDS             complement(2094..>2783)
                     /locus_tag="OQN12_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1656196.1"
                     /translation="RIFNSSFGAIFRRSEECLHIIPTRENEGENGDIGPLRPFTLNLR
                     TGRISMGHGLDVTGDIFANRFAINSNNGMWIQMRDNNAIFGRNIVKTDSAQALLRQDH
                     ADRKFMIGGLGNRQFGIYMINNSRTDNGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSR
                     YVKDVRLGSQQYYGVNNWQTWNFQCPSGHVLSGINVQDTGSNSADNIAGVYYRPVQKY
                     INGTWYNVASV"
BASE COUNT          795 a          588 c          611 g          789 t
ORIGIN      
        1 gggtgtcgtt tgatggttct aaaaatattg agctaacggc ggaagattta aatcttgagc
       61 aaactgtaga attagccgca ggagcattgc agaaaaacca gaacggcgca gatattccgg
      121 gaaaagatac cttcactaaa aatattggtg catgtcgcgc ttttcacagt tctattagta
      181 caggtgcagg gaactggaca acggcacaat tgattgaatg gctggattct caaggggcat
      241 tcaatcaccc atactggatg tgcaaatgtt catggtcata cgccaataat aaaattatta
      301 ccgatactgg ctgtggaact attcatcttg caggttgcgt tattgaggtt atgggtaata
      361 aaggtgccat gaccatccgt gtaacaacac caagcacttc caccggaggc ggcaccacta
      421 acgctcaatt cacttatatt aatcatggtg atgcttatgc tcctggctgg cgaagagact
      481 acaacacgaa aaaccagcag cctgcatttg ctttagggca gacaggaagc acagttggaa
      541 atgacaaagc tgttggatgg aactcgatta gcggggttta taacgctaat attggtggag
      601 caactacgtt gattctccat ttttatatgg gagctggtag ttgtccggct gttcagttcc
      661 gcgtgaatta taagaacggc ggtatttatt atcgttctgc gcgtgacggt tatggttttg
      721 aagccgactg gtcggaattt tatactacaa cacgtaaacc atctgcggga gatgttgggg
      781 catataccaa agcagaatgt gattcacgat ttattacagg tattcgatta ggtacaaaat
      841 cgtctgtgca aacatggaat ggtcctggat ggaacgataa atctggttat gttgttactg
      901 cctcaattaa ctcaaataag gacgaactta ttgatacaac tcaggcaagg ccggtgcagt
      961 attgtatcaa tggtacatgg tataacgcgg gaagtattta attatgcagc atttaaaaaa
     1021 catcagatca ggtaatccaa aaactgttaa acaatatgaa ttgacgcgac aatttggtgt
     1081 tgtctggttg tattctgaag acggtaaaaa ctggtatgag gaacaaaaga acttccagga
     1141 tgacactata aaaattgctt acacatcaga tggagttatt gtggctataa ataaagacgt
     1201 atctacaata aatccagaag gtttaagtgt ggttgaggtt gctgacatta ccgcaaatcg
     1261 ccgtgctgac atttctggta aatggatgtt caaagatggc gtagtgataa agcgaactta
     1321 taccgaggaa gaacagaggc aacaagctga aaatgaaaaa caaagcctat tgcaacttgt
     1381 cagggataaa acccaactgt gggactcaca gctacggctg ggcatcattt ccgatgagaa
     1441 taaacagaaa ttaaccgcgt ggatgctata tgcgcagaaa gtagaatcta cagatacctc
     1501 catcctgcca gtaacgtttc ccgaacaacc agaatgagaa aaggcccgtt atcgggcctt
     1561 atttttactc aggtttttgt ggccattctg gctttgccgt atccacgcgg cttacaagaa
     1621 cgctgtagcg ttcccatgct tccagtcgtg tgcgctcctc gtctgttgcc atattcagcc
     1681 tgacagcgcg ttccagcggc tggatgactg attcagcttc ggaaagcaat gcggcctttt
     1741 gtgattcggc ctgttgttgt tgctcgtctg ccgtataaat ccgtttaact acagctccat
     1801 ccttaaacat ccactttcct gattcatcag cgcggcggtt ggctgtaata tctggaacct
     1861 caacgacgct aaaaccttca gggttaagcg tggaggcatc tttagtgatg gagacaataa
     1921 tattatttgc atcgtaaaca atctttattg tgtctggctg aaagtttttc acttcctcat
     1981 accagttttt tccgtcctca gagtaaagcc agataactcc gtgtttcttt gttaacttat
     2041 actgttccag tgttttagcg ttacccgctt ttatgttctt taagtgcatc atattaaacg
     2101 ctcgctacat tataccaggt gccatttata tacttttgaa cgggtctgta ataaacgccc
     2161 gctatattat cggcagagtt ggaccctgtg tcctgaacat taataccaga caatacatga
     2221 cctgacgggc actggaaatt ccatgtttgc cagttgttca ctccataata ttgctgtgaa
     2281 ccaagtcgaa catctttcac atatctggaa tcaaaattgc catagttgcc gggaataact
     2341 tgcgagccgc aaagccaatt cccgttatta tccatgtacg cctgaccatc ggtgccattg
     2401 tctgtccttg agttattaat catgtagatg ccaaattgcc tattccccag tccaccaatc
     2461 ataaatttgc ggtcagcatg gtcctgacgt agcaatgcct gggcgctatc agttttaact
     2521 atatttctcc caaagatagc gttgttatcg cgcatctgaa tccacatacc attattgctg
     2581 ttaatagcaa aacggtttgc aaatatatcc cctgtaacat caagaccatg ccccatgctt
     2641 atccgaccag ttctaagatt aagcgtaaag gggcgtagtg gccctatatc accattttct
     2701 ccctcgttct ctcgtgtagg aatgatatgc aggcattctt cagaacggcg aaaaatggca
     2761 ccaaaagatg aattaaatat cct
//
LOCUS       DAJVRP010000215         2690 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVRP010000215 DAJVRP010000000
VERSION     DAJVRP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.222"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            230..769
                     /locus_tag="OQN12_004072"
     CDS             230..769
                     /locus_tag="OQN12_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS1656197.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="OQN12_004073"
     CDS             complement(995..1318)
                     /locus_tag="OQN12_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCS1656198.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="OQN12_004074"
     CDS             1604..1798
                     /locus_tag="OQN12_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1656199.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAJVRP010000216         2685 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJVRP010000216 DAJVRP010000000
VERSION     DAJVRP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.223"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..191)
                     /locus_tag="OQN12_004075"
     CDS             complement(<1..191)
                     /locus_tag="OQN12_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656200.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEA"
     gene            complement(191..1813)
                     /locus_tag="OQN12_004076"
     CDS             complement(191..1813)
                     /locus_tag="OQN12_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HCS1656201.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(1816..2388)
                     /locus_tag="OQN12_004077"
     CDS             complement(1816..2388)
                     /locus_tag="OQN12_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCS1656202.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(2372..>2685)
                     /locus_tag="OQN12_004078"
     CDS             complement(2372..>2685)
                     /locus_tag="OQN12_004078"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1656203.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT          603 a          746 c          636 g          700 t
ORIGIN      
        1 gcttcgccga taatccagga cgaacgatag gaccattcca ccagcatgcg gttacgactg
       61 gtgaaattag cccggtaggt ggatgctgag tgctggttag gtgtctgcat ccccacgcgg
      121 gcaacaaagt tctcatagcc atcagcggtg gcctgtgccg ttcgccgcgt ggcttgtttg
      181 tttcgtgcca tcaggcctgt ctccctagca gctcccagat gttcagggct gaattcattg
      241 gcgcgtagct gatcatcacc gagtcggcaa ggtttggcga ccgggttcca tcaggctgtt
      301 tatcaataac gatttttccc acaccattaa tggaataggt cggctgcgaa agctcgatga
      361 tgagtttatc tttgagtgcc atgctactgc tgattgagat gatttcgtcc gggttgtaat
      421 ccataccttc aaccacggcg cgccaggtat tctgaaaaag tttacgtaac cgccaccagc
      481 tctgggcttt ggcgttagcg aagaagtcct tgttcaggcg ggcggcctgt ccgttgtcac
      541 cgcgcaccgc ttcgtcgtcc ggatcaaata ccgcgccact acctcgaaac ggtgtggcga
      601 gtattgacgg tcggcgcgca gcgttacgca gttcgttgat agcgcgtgca tcgccgcgaa
      661 cgccagcgcc caacccgtcc tcgtcaaagc gaaactcttc gaggttgtcc tgttcgcaaa
      721 agccgaaaac cttctcgacg gactgataaa tgtcgctgcc cacaccggac cattcccgca
      781 cattctccag gaggaagcca tgacgggtgg aaaaggaatt tttgtccctg ccttcgtcgg
      841 cgacgtccat cgcgcccagt cgcttgcctg ttggctggat gcccagcttg atatgcgcgt
      901 cgacagcagc ctgtacccat tcggatggaa tcaggacgcc ttccgctgat gcactgtagt
      961 tcaggtcaag ttcctgtgcc accaccaccg gattgtcgat tttctcgcat tccctgcgat
     1021 accactcttc atccttgcga ggatcatccc gccagtggaa tgtgaatacc ggtatcttcc
     1081 cgccatgacg cttctgagcg aacgggttcg ccatgccgtt aactgaactc aggtcaatac
     1141 ggcaacgcgt cgtttgtgac aacgccgcat caatcagcag aggacgctga aggaatgcag
     1201 actcatcaac cagataaagc gtggtacggt caccacggcc aatattatcg ccagcctcac
     1261 ctttgataac ggcaccagtt tcaggaaact caacgcgcat gtatggtgcg tgcttctttt
     1321 cgtcccacga acctcgaaac tctacaggta gtgtttccac gaacttgcgc gccttccaga
     1381 acaatgcttt cgggtcaccg gtgctgtcga cgtattcctc tttacgggag ccgaaaccga
     1441 taaccatttc tttgttgaag agacaaagcg agcatgccag cccgatcgcg gtccaactga
     1501 gccccatttc acgggatttt tcggtaatac cattctcccg attaccccag cgttccataa
     1561 tccagtggat ccactcctcc tgcttaggga agagtaaaaa cggaatggtc accggcaggc
     1621 cataatcaat attacgcggg tccgttgtca tgccccagtc gatgatgaac tgagccggat
     1681 tggttcggta aaactgtttt agtgcaggca atatttcagg attctggcga atgcgctgta
     1741 ggcgttccat ccgccattca aaaaccatct ggtaatcagg atgtttaaaa tcgaagggga
     1801 atggtaacgg catacttagc ccatcatttt tctatacgcc tctgcagcct gctccggcgt
     1861 taagttggta atttctgttc tgactggtcc tccgtcagca ccagtcactt catttttgac
     1921 attgtcttta aacgcctgaa cagaaacatg acgcccgagc aactcaaggt ttttaacctt
     1981 atcaggccat ttgattttct tcaggagagc agcactatct gctgatgcca tctcgacgac
     2041 atccattcct gatagcgttg tacgccacac cttaggccag tctttaatct gcttcagttc
     2101 gccattggca agcagaatgt caagcacgtc catctgatcg atttcagtca gccgtcgcaa
     2161 tacataggcg gcgtctatac ctacctcttc attgcgttca gcctttagct cagcaatgcg
     2221 atctgctatg tcaggttttg acaggttctc gctaccagtg gcgcgagcag ttttctcgct
     2281 gtaacccgcc ctgatagctg cctgagtggc gtttaaatct tttaaatatt ccctgcagaa
     2341 cagttcctgc ttaggtgtga gctgtttcaa atcacacctc cgaaacattg ctccaatttc
     2401 tatgattaac gatgtcagca atcatggtag tggatacgcc atatctcttt gctaaagacc
     2461 taagcccatt acctacactc ccgcgcacat actcgcggcg aatgcagatg acatcgcact
     2521 cttttaattt cgccattccg ttcttttcac ctgaacggtc agggtatgag tttctgcctc
     2581 gccttgacct gtcacgatta ttgtcagcga cagtacctat aatcagatgg tcaggattca
     2641 cgcatgatgg attatcgcat ttatgcctaa caacaaggtc cttga
//
LOCUS       DAJVRP010000217         2656 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVRP010000217 DAJVRP010000000
VERSION     DAJVRP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.224"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(68..592)
                     /gene="sat2"
                     /locus_tag="OQN12_004079"
     CDS             complement(68..592)
                     /gene="sat2"
                     /locus_tag="OQN12_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1656204.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(687..1160)
                     /locus_tag="OQN12_004080"
     CDS             complement(687..1160)
                     /locus_tag="OQN12_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1656205.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1492..2028
                     /locus_tag="OQN12_004081"
     CDS             1492..2028
                     /locus_tag="OQN12_004081"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCS1656206.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2143..2469
                     /locus_tag="OQN12_004082"
     CDS             2143..2469
                     /locus_tag="OQN12_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1656207.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
BASE COUNT          730 a          603 c          559 g          764 t
ORIGIN      
        1 cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg tcggcttgaa
       61 tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
      121 tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga gtaaagccac
      181 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
      241 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
      301 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
      361 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
      421 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
      481 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
      541 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc atatgacgcc
      601 taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggtacaaaa ggcgtgacag
      661 gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta actataattt
      721 atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat ttcaggaaag
      781 taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc tacttgatcg
      841 atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt tattttcttt
      901 aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt gtcagatgta
      961 aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat tgattcaaaa
     1021 gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag gagctgttca
     1081 cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt cgatatagct
     1141 accattagtg atagtttcac aattcttcct cagaggttaa cattttatta gtaagcatgc
     1201 tcgttttgtc acccgctgat gcttaccgtt aattaatata atcacttgta ttaaatagac
     1261 tttctttaaa aatacaagac actctgttat tacaaatcgt gcatgccgtc tatcctgctt
     1321 gcacgatgca cattatctca cccaaagaac tcataacatt ttaatttatt tagcattaaa
     1381 ttaaaactct accggtaaac aagcatctct aggcgtaaaa aacaggcact tttttaaatc
     1441 ccagctagaa taggctgtat aggcagacag ttgcaagaca aggagcgttt tatgtctaac
     1501 agtccatttt taaattctat acgcacggat atgcgacaaa aaggttatgc gctgaaaact
     1561 gaaaaaactt acctgcactg gattaagcgt tttattctgt ttcacaaaaa acgtcatcct
     1621 cagaccatgg gcagtgaaga ggtcaggctg tttttatcca gcttagcaaa cagcagacat
     1681 gtagccataa acacgcagaa aatcgcttta aatgccctag cttttttgta caacaggttt
     1741 ttacaacagc cgttgggcga tattgattat atccctgcaa gcaagcctag acggctaccc
     1801 tctgttatct ctgcaaatga agtgcaacgc attttgcagg ttatggatac tcgcaaccaa
     1861 gttattttta cgctgctgta tggtgcaggt ttgcgcatta atgaatgctt gcgtttgcgg
     1921 gttaaagatt ttgattttga taatggctgc atcactgtgc atgacggtaa gggtgggaaa
     1981 agcagaaaca gcctactgcc cacgcgccta atcccagcaa taaaataact cattgagcaa
     2041 gcgcggctta ttcagcaaga cgacaactta caaggcgtag ggccatcgct gccttttgct
     2101 ttagatcaca aatacccttc tgcttatcga caagcggcgt ggatgtttgt ctttccctcc
     2161 agcacgctct gcaaccaccc gtataacggc aaattatgcc gccatcatct gcatgactcc
     2221 gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag gcatcgttag caagcgtgtc
     2281 acttgtcata catttcgtca ctcgtttgct acgcatctat tacaagcggg gcgtgatatt
     2341 cgcactgtgc aagaactctt agggcataac gatgttaaga ccacgcaaat ctatacgcat
     2401 gtgttgggtc agcattttgc cggcaccacc agtcctgcgg atggactgat gctacttatc
     2461 aatcagtaaa agtagataaa tatctttatg tagccctgag tagcccctga tactttttat
     2521 ggtgaaaaag ataaacaatc atagagggga taaatatgag ccagttctac cattgttcag
     2581 ataagtttaa gcaagatact gtcaatcaca gtgaccattc atggtcatta cacttcctcc
     2641 agttgccaga caatgc
//
LOCUS       DAJVRP010000218         2573 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVRP010000218 DAJVRP010000000
VERSION     DAJVRP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.225"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(64..822)
                     /locus_tag="OQN12_004083"
     CDS             complement(64..822)
                     /locus_tag="OQN12_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1656208.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OQN12_004084"
     CDS             complement(830..1933)
                     /locus_tag="OQN12_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1656209.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2573)
                     /locus_tag="OQN12_004085"
     CDS             complement(1943..>2573)
                     /locus_tag="OQN12_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1656210.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          710 a          683 c          602 g          578 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggtggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aac
//
LOCUS       DAJVRP010000219         2564 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVRP010000219 DAJVRP010000000
VERSION     DAJVRP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.226"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..775)
                     /locus_tag="OQN12_004086"
     CDS             complement(<1..775)
                     /locus_tag="OQN12_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCS1656211.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSD"
     gene            complement(779..1522)
                     /locus_tag="OQN12_004087"
     CDS             complement(779..1522)
                     /locus_tag="OQN12_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCS1656212.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(1413..>2564)
                     /locus_tag="OQN12_004088"
     CDS             complement(1413..>2564)
                     /locus_tag="OQN12_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCS1656213.1"
                     /translation="DVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLD
                     RWMVDPALTRRIKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKM
                     TENEWGMSVVERIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEAL
                     LKNIDLIRQFQSNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLF
                     GQSPKGFSTGDADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFN
                     PLWQMSDVDRSTVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIE
                     NAEDEAPPGIGEPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
BASE COUNT          529 a          753 c          648 g          634 t
ORIGIN      
        1 catcactgtc gccggttgga tctccgccca tctctggtgc tttgtccggt agtggttgct
       61 gcggggacag gttgatgttg agattaacgc cctgcggcaa atccccctca tctcctgtaa
      121 ccgatgcggg agccgactcc accagttcgt tcatggtgtc ggcatctcct gtcttgatgg
      181 ccgcacgcat gcggttccac cagtttttct tttgatttgc cattgtgtct ctgtctccaa
      241 ttgcacaacg atttccggct ctgcctttag ggacaagagc cacatggttt ccggtaatat
      301 cgacctgctc ggctttacat ggctcggtct gctcgtactc cgcgtcatag ccgcacgaca
      361 cttcgcgcag gccatcttcg ataagctgaa tggcgctttc gtctttgacg ataaggtcag
      421 ccagcatcaa atcagactgc tcacccgtcc cgcgccggac attctggagg tgcccgacag
      481 caagcacttt ccagttctcg ggatttacca gccgcacatt cccgttttca tcttcaggat
      541 gcaggatcgt gatgctcatc ccttcgaatg aggcaagcgt ggccggatgg aatacctgct
      601 caggagaacg cgtgacgact atttcaacga acttatcggg tttcagtttt ggcaggtcat
      661 cagcaccata gagctgctta cctgttcgtc ctatcggcac gtctttgcac agcaacgagc
      721 cgtcagccag gcggtatcgg gtttccccga gcctggtctc aaaaaagtat ttcatgggtt
      781 atgctgccct tttcattgcg aatggaacgc gaacaaacac gatctcttta tagcagcggc
      841 agtttggcag ctcgcccgcg tggccggtca tgccgtccaa agttggaggt gaatcccagc
      901 ggacgaattt acctttcatg tgatcgtggg aatctcggac atcgccatca tcggctgtac
      961 gccaaatata accatccgag ccaatagcta tcgctcgggc catatccagc gcgcccgttg
     1021 ctcttcccag ttccgttcgg gcaatcagat cggccctaga cttagcaaca tcgccggtag
     1081 atgcaatctc tttagcaaac tgctccgccc gaccaccggt gacaacagcc tctatcgctt
     1141 tgttctggat gtcgtacacc ctgtctgcgg cctcgagagg cagagatttg atgtacttga
     1201 cctgttcggc gatgatggat tgcatcacct ggcccacagg agcgctttcc acaagattgc
     1261 ggagctcgcg actgatgttc ttgctgtgtt gccgccaaac tttctcgttc tgccgggtta
     1321 ggtccgcagt aaagttttcc gcgacctttg tcgcccaggg ggtgatgatt tcactgtaac
     1381 gctccagcgc cgcaataatt tccgtgatac tgtcatttga accatcgtag cgaccattta
     1441 cgatgtctcc gaccgccctc gctatcctgc gtaggctggt tcgataccgg atttccgcct
     1501 gacggctcct gcggttcgtc atcaggttcg ccgatgccgg gcggcgcttc atcttcggca
     1561 ttctcgatgt cctcgttggt aatggatgcc cctatgccgg ttacgtcaga attttctcgc
     1621 aaatcagtca tagcggcttt cagtgtcatc agaccatcac ccagcgccgt actgattgcg
     1681 ttggtggtgt ttaacgccac cgttgagcga tcgacatcag acatttgcca gagcgggtta
     1741 aactcaaacg tgaaatcgtc cggcagcggc ttgccaagtt ccgaacgatg catgatgtcc
     1801 agtatccgcc gcaccggaag acgtaaacgc ctctcctgca acgaactgat gcgatcgtaa
     1861 tagttggcaa ggtctgcatc accggtagaa aatcctttcg gggactgtcc gaacaaccgc
     1921 accagtggga taccaacagc gccactaatc tgttctgcaa actgcgaaag gatgtcatcc
     1981 agaccactga agctgtactg atgggtttca aacttatccc gcgagtccat gagcgtcatg
     2041 ccttcattac tctggaactg tcgaatcagg tcgatattct tcagcaacgc ttcatacgca
     2101 ggaccaccaa gtgcgataag ctcgcgtagc ttctccacgc tgtaggtacg caaatgcgcc
     2161 ttgtagacca gctgcgccgc gccgacagta gcgctgtcga acgcggtaag acgatcccag
     2221 atacgctcta caaccgacat tccccattcg ttctcggtca tcttctgctg aaatggcaac
     2281 gtgacgccat caaagcgaat caggcgactg tgatgaatgc gccaggcagg aattcccgtt
     2341 gctgtggtca ccacatcgta aaactcaggt ttacccaggt ccggccccat atctttaatg
     2401 cggcgggtca gtgccgggtc gaccatccag cggtcgagcg ggagaatccc cttaaacttg
     2461 cccttaccga tggtttcggg tcgcagcggg gtcattggtg cctgcccctc aatcatgatg
     2521 aaacccaccg cgccgccgta gaggcgcgac catttcagca cgtc
//
LOCUS       DAJVRP010000220         2442 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVRP010000220 DAJVRP010000000
VERSION     DAJVRP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.227"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            84..707
                     /locus_tag="OQN12_004089"
     CDS             84..707
                     /locus_tag="OQN12_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1656214.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
     gene            complement(761..1933)
                     /locus_tag="OQN12_004090"
     CDS             complement(761..1933)
                     /locus_tag="OQN12_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1656215.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2282..>2442)
                     /locus_tag="OQN12_004091"
     CDS             complement(2282..>2442)
                     /locus_tag="OQN12_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1656216.1"
                     /translation="EMGGWESIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSR
                     NKEGTNDV"
BASE COUNT          738 a          445 c          504 g          755 t
ORIGIN      
        1 cttgtgaagg aaatggtact cagggggatt ccagtacagg tggtgcgcaa ttagctataa
       61 atggccttaa cggttcgtgc gctttggtat taaacaaccc atactttgag gctaatgccg
      121 ggggggcaga ccttgctata gataatactg ggacgcgccc tgtaactgtg gttataaacg
      181 gtggaaattt tcatcgcgtg agcagtgcaa gatacacgca cacgaatatt caagtaacaa
      241 gctcaggtgg gggaaaagtt acagtcatac tcaacggcac aacgttccag agcgcagggg
      301 attatcagcc aagtgcagaa cgaccatact ggataacagg agctaattgc gaggtagttg
      361 acattgggtg tgtttttaca gaaacaacca gtaaggtaac atcggcttct gcattgtcag
      421 tcacacgctc gggaaaaatt aacgccaatg gtagtatcga cgttgctact ggtgtttcat
      481 cggttaacgt ggtttctaca ggtgtgtatg atgttagctt ttctcatccg ctcgcagcag
      541 ttgctagcgg ttatattgtg cagataacac ctatatctgc gcctgattca gttagttgtg
      601 atgttactta tattggcgta gatacattta gggtgaccct gcgcaatacg cttagcgggg
      661 caggaatctc cagttcgttt gcatttagca ttaccagact aatataaata cattcgccgc
      721 cccatttgga catatatgta gggcggctta tgtttattgt ttattgattg ttgtttgtta
      781 atgtgggtat taattctttt actaaagttg tggtttgttt tgttaatttg atgaatggcg
      841 tctctattaa tttaaaagtt aggcatgatg tgaacgtaat aagaaatgca gttgtgctta
      901 ccagtatgat aaatgtattt tcagtgtaat tattaggaat aatccacgtc attaggcaat
      961 aaaggaatat cccatgcaga aggtaaacgc tgtaggtagt ttctcctagc tttctggtta
     1021 tatttaatct taatattcca tacaaatcac aacctgatgc gataatgata aatgttattc
     1081 cacaaagaat cagtggtagc ggtgcgtaag ttgttttgaa atacgtcatt tcaaaaacca
     1141 ttatcgcagt tattataatt ggtgttactt ttgctttggc aattccattg actatctttg
     1201 atttatttag caaaaatgcc aggagcccaa aaagaaatga tgctatgtgt attttactga
     1261 aaaataaaat gaaaacaaat agcgatattg cgctaatcat aaccatgtaa atactgactt
     1321 ttctttttat gagcgcggaa attaccggaa gagaaaaata aaagaaccat tcatatacaa
     1381 gtgtccatgt tacagcggca ttgatagtaa atgaatcttt tacgtcatta atgttcggca
     1441 tacctagcgc agtgaatggc aaccacttca ttattgacac aaaaagctct tctgtggata
     1501 cctgcattct ccatccagac ttaaaaccta caatgataaa aatgagacat aggctaacta
     1561 tgaacattgg ggttaatcgt agtaatcttg atacataaag ccttgtccag tcctgatctc
     1621 cagagataat ctttgaaaag aacagataac cagtaatcat aaaaaagaat gacacaccaa
     1681 cttggcctaa atttgctaac agatttgatg aaggtgcttc ccatactcca gatgacaagt
     1741 atccattcca aattgctgcg tgatgaataa gcacgaaaat tgctaagaac ccccgcattc
     1801 catctatttg attattcctg ccgtgctcaa caagatttat tggggaaatt ttatcaaata
     1861 accccacagc taataacgac atcaatatca cggatgttat taataataaa ctgttaattt
     1921 caatcatatg catgctctat tacgcaatta aaatttctcc aagtatactg acttttagtt
     1981 aatgatcata tagttatgtg taactgatga aacaaaacgg agacacacca agctttgcac
     2041 tggattgcaa ggctttgtgc tcttctgtga atgtgtggct atatgtttga agatcgttgt
     2101 gccgtatttg tgacatatat atggcaacat catgcatcaa ctttctgttt gtgtcatcaa
     2161 ctatagctta gtgaatgcgg ttaatgcttg ctaaaacaga tagttatgat tggtgctaca
     2221 gattcgtaat gcgaaggtcg taggttcgac tgctattatc ggcaccattt aaatcaataa
     2281 gttacacatc attagtacct tccttatttc ttgactggga caaatttggg accgatgggt
     2341 tcagaatcaa gtctatttgc cgtgcgtgtt cggtaaggtg attaggtgcg aggtgagcat
     2401 atcgacgaac catttcgata gactcccagc ctcccatttc ct
//
LOCUS       DAJVRP010000221         2395 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVRP010000221 DAJVRP010000000
VERSION     DAJVRP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.228"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            65..1543
                     /locus_tag="OQN12_004092"
                     /pseudo
     CDS             65..1543
                     /locus_tag="OQN12_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1540..>2395
                     /locus_tag="OQN12_004093"
     CDS             1540..>2395
                     /locus_tag="OQN12_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCS1656217.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQW"
BASE COUNT          616 a          570 c          594 g          615 t
ORIGIN      
        1 tactcgtgag gatttatgtt ctgctggcag tttcgtgatc aacacctcgc gccaggatgg
       61 ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt tcattgtttt tatcgtcggc
      121 atggggatgt cggctttccg cacctgaaca taatatttac cagaaacagg gaaaaggcgt
      181 ggtgtacctg cgcccttata aagaaacaaa tctttctcta cctgacgtga attacaagag
      241 tttgcgtcgg ttacccaatt tattaataga cccgacaaca ctagacgaat gggataaaga
      301 accgcctctg actgatttga caaccgacta cctctatgag ggcgcacagg cctggtatcc
      361 tcattattca tggcatagcg atggtcgcaa tatcctctac gcgggtgaag ttgtgcaaaa
      421 ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag gcatggggtg attttgccgc
      481 agataaacac agtctctatt ttgaaggcaa gcgcaccgat gacaatggtg gtggaaatag
      541 cttggatatc aaaacgctgc atcaggtgga attccggcct ccctgggatc ctgacttact
      601 ggggctgata ttacgtgacg ctaatttctt gtatatcaat gggcatcgcc tcgctgaccc
      661 cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag cgaggaaagt tttccaccac
      721 gtttaacccc tgcattgccg taccatttgg cccctgggat accctggctc gtacacggac
      781 caaaatcctg ctcaacggcg aacagcttga tgccgacccg gacacctttt ccgtcgtgcg
      841 ctggatgccc ggctcactct tgacctggcg tgataaaaac gggctgcagc gtaaagtcct
      901 cgacaaggaa aatctggcgt gggatgaaga tttaacaaag cactgtctgg atttttctct
      961 gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt aaacaggaag aattacccgg
     1021 actcgatccg gagcagtttc aacccatcag tgatgctgtc gcccagcatc aggactcgct
     1081 ttataccatc atcgaaacag agtctggtga ccgtaagctg gagatcgtga aacttgatga
     1141 tcccaatctt attatcaaca aacgtttcaa cgccgggaaa cgccacggct atttacttac
     1201 gcgtgccgaa gggtagccat accattccgg tttacacgtg tttgaatctg acggaccgct
     1261 gatcttactg gataaccgtt ctccggatga acgcgaagcc catcttaatg accatccctt
     1321 tttgcgcaga tggtatgccc gcgataaccg ctacatttac agctttgatg gcgcgcagct
     1381 ctggcgatac cgcaccgctg atccgaaaca agttcgctta atctggaagg aacaacattc
     1441 gggatatgtc tatggcgtaa attacaaaac gggatatctg gacggaaaaa ttactgatga
     1501 cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa tgaagggtca aataatcaca
     1561 gttgcactca tcctgttggg acacacattt ctgacgcctg ctgtacaggc catagggttc
     1621 gactattaca atgatcacgg tgttatgtct tacggtaaag gttattgggg ggataagaaa
     1681 atcattgccc agtttccgga gatgagcagg gccggtttgc gccttgtgaa aaatatctct
     1741 gggaaaacgg gttatatacc ttccgatgaa ataagcatag aaaatgagga ataccactgg
     1801 gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg ttagcaatcc accgggaaca
     1861 ctcactgtcg atattgccag ctttcaggct atgggccgct tcgcggtcga taaatatagc
     1921 ctctattttg acggacagcg caccgaaagt aatagcggcg cgtcacgtgt tgagctggcg
     1981 acactaaaag ctatcgaagg taactctacc acgctggtgg atagtaaaaa tctctacttg
     2041 tccggtcgac gtcaggggag cagcagtgat gttactgtat tagaaaaaag atggtggggt
     2101 attaatccac gtcttatgag tgtaaacaga aattcatatt ccaatgatct gcttatccgc
     2161 agtgggcaga atatttattt aaatggcgtt caccttacgg cgaatgcaga ctcatttgag
     2221 ataattcgct ggatacctca ttcactgctg gtttttcgcg acaataaggg tctgcatcgt
     2281 tatccctttg gtcaattatc aggcaaagcg atacccgtag atgatgacgt ctcttttgaa
     2341 gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgc ccgaccgtca gtgga
//
LOCUS       DAJVRP010000222         2236 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVRP010000222 DAJVRP010000000
VERSION     DAJVRP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.229"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            54..2236
                     /locus_tag="OQN12_004094"
                     /pseudo
     CDS             54..2236
                     /locus_tag="OQN12_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          599 a          497 c          562 g          578 t
ORIGIN      
        1 tcagtgaccc gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga
       61 cgaccagaat agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga
      121 tggccgaggt cagtgtattc aatccggcgc ttctggaaat caaccatcaa tccggagtcg
      181 atattcgcca gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt
      241 ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg
      301 ctgatctgca cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag
      361 ttgatgcgct caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac
      421 gtattgaagg ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc
      481 accaaatcta catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg
      541 ggattaatgc gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt
      601 atggttcaca agagacagat accagttatc tcaatctgcg caatggactg aatattggac
      661 cgtggcggct acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact
      721 ccattagtac ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg
      781 atacgtggac ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata
      841 ctgataacga tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg
      901 caaagtccaa cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtcagccg
      961 ttccacaagg tgcttttgag atcaccgatc tcaacaccgc aagtacaggt ggcgatttgg
     1021 acgtaaccat caaagaagaa gacggtagcg aacaacgatt cacccaacct tatgcttcat
     1081 tggcgattct taaacgtgaa ggtctgacag atgttgatgt cagcgtgggt gaattgcgcg
     1141 atgaagacgg atttacaccg gacgtccttc aggcgcaaat acttcatggt ttttcccacg
     1201 ggatcacttt atatggaggt atgcaggctg ctgaaaatta tggttctgca gctctgggtg
     1261 tcggtaaaga tcttggcgct ttgggcgcaa tttctttcga tgtgacacat gctcgtgcga
     1321 attttagcca tgatgataca gaaacgggtc agtcatatcg ctttctctat tcaaaactat
     1381 ttgacgacac agacactagc ttgcgcctgg ttggctatcg ttactccacc gagggctact
     1441 ataccactca atgagtgggc atcgcggcgc aacagccctg aagacttttg ggaaacaggt
     1501 aaccgacgta gtcgcgtgga gggaacgcta acgcagtcgt tggggagaga ttatggcaat
     1561 ttatacctga cattaagccg gcaacaatac tggcataccg atgatgtcga acgattaatg
     1621 caatttggct acagcagtag ctggaagcgt ctctcgtgga acgtctcctg gagttattcc
     1681 aatactgcca gacaggggac ggggaacaac catgccagtg ataacaccag tgagcagatc
     1741 tacatgctct ctttatctgt tcctttatcg ggctggtggg gtaatagtta cgccacctat
     1801 tctgtttcgc aaaacgataa ttccggtagc tcacatcaac tcggactcag cggtacggcg
     1861 ctggaaagaa ataacctttc atggaattta atgcagtcct ataacagtca tgatgatgag
     1921 gttggcggta atatgtccct gacctatgat ggctcttatg gcacggtgaa cggcagctat
     1981 aactacagcc aaaattccca gaggctgaat tatggtatca gagggggaat tctggcacac
     2041 agcgaagggg taacgttaag tcaggagtta ggtgaaacta ttgctcttgt taaagcacct
     2101 ggggccgccg ggttagaaat agataatatg cgcggtgctg cgacggactg gcgcggctat
     2161 acggtcaaga cacagctaaa cccttatgat gaaaatcggg tagcaatcag cgataactat
     2221 ttctcgaagt cgaata
//
LOCUS       DAJVRP010000223         2228 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVRP010000223 DAJVRP010000000
VERSION     DAJVRP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.230"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..898
                     /gene="ssuA"
                     /locus_tag="OQN12_004095"
     CDS             <1..898
                     /gene="ssuA"
                     /locus_tag="OQN12_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCS1656218.1"
                     /translation="AESSPEALRIGYQKGSIGMVLAKSHQLLEKRYPQSKISWVEFPA
                     GPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVGVEPPKPKAEVILVAENSPIKT
                     VADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQPTYLTPADARAAFQQGNVDAWA
                     IWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPYAEKNGAFIQGVLATFSEADAL
                     TRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKPVNAEVAALQQQTADLFYENRL
                     VPKKVDIRQRIWQPTQLEGKQL"
     gene            895..2040
                     /gene="ssuD"
                     /locus_tag="OQN12_004096"
     CDS             895..2040
                     /gene="ssuD"
                     /locus_tag="OQN12_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCS1656219.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2049..>2228
                     /locus_tag="OQN12_004097"
     CDS             2049..>2228
                     /locus_tag="OQN12_004097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCS1656220.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          497 a          607 c          633 g          491 t
ORIGIN      
        1 tgcagaatcc tcgcctgaag cgttacgtat aggctatcag aaaggcagta ttggtatggt
       61 gctggcaaaa agccaccagt tactggaaaa acgctatccg caatcaaaaa tctcctgggt
      121 ggagttcccc gcgggtccgc aaatgttgga agcgttaaac gttggcagta ttgatctcgg
      181 cagtaccggg gatattccgc caatctttgc ccaggctgcc ggggctgatt tggtatatgt
      241 gggcgtcgag ccaccgaagc ccaaagccga agtgattctg gtggcagaaa acagcccgat
      301 caaaaccgta gccgatctta aaggtcacaa agttgccttt cagaaaggtt ccagttcaca
      361 caacctttta ctgcgtgcac tgcgtcaggc cggacttaaa ttcaccgata tccagcccac
      421 ttacctgacg cctgccgatg cccgcgccgc gttccagcaa ggtaacgttg acgcctgggc
      481 tatctgggat ccctactact ccgctgcgtt attacagggc ggcgtgcggg tgctgaaaga
      541 cggcaccgat ctcaatcaaa ctggatcgtt ttatctggca gctcgtccct atgcagaaaa
      601 aaacggcgct tttattcagg gcgtactggc aacctttagt gaggccgatg cgttaacccg
      661 cagccagcgc gaacaaagca tcgctttact ggcaaaaacg atgggcttac cggcaccggt
      721 gattgcctct tacttagatc atcgccctcc taccaccatc aaaccggtta acgccgaggt
      781 tgccgcctta cagcagcaaa cggcagatct gttttatgaa aaccgtctgg tgccgaaaaa
      841 agtcgatatt cgccagcgca tctggcagcc cactcaactg gaaggaaaac aattatgagt
      901 ctgaatatgt tctggttttt accgacccac ggtgacgggc actatctggg aacggaagaa
      961 ggttcacgcc cggttgatca cggttatctg caacaaattg cgcaagcggc ggatcgtctg
     1021 ggctataccg gtgtgctgat cccaacgggg cgatcgtgtg aagatgcgtg gctggttgca
     1081 gcgtcgatga tcccggtgac gcagcggctg aagtttcttg tcgccctgcg tcccagcgta
     1141 acctcaccta ctgttgccgc ccgccaggcc gccacgcttg accgtctctc aaatggtcgt
     1201 gcgttgttta atctggtcac aggcagcgat ccacaagagc tggcaggcga cggcgtgttc
     1261 cttgatcata gcgagcgcta cgaagcctcg gcggaattta cccaggtctg gcggcgctta
     1321 ttgctggggg aaaccgtcaa tttcaacggt aaacatattc atgtgcgcgg agcaaaactg
     1381 ctcttcccgc cgattcaaca gccttatcct ccgctttact ttggcggatc gtcagatgtc
     1441 gcccaggagc tggcggcaga acaagttgat ctctacctca cctggggcga accgccggaa
     1501 cttgttaaag agaaaatcga acaagtgcgg gcgaaagctg ccgcgtatgg gcgcaaaatt
     1561 cgtttcggta ttcgtctgca tgtgattgtt cgtgaaacta acgacgaagc gtggcaggcc
     1621 gccgagcggt taatctcgca tcttgatgat gagactatcg ccaaagcaca agccgcattc
     1681 gcccggacgg attccgtagg acaacagcga atggcggcgc tacataacgg caagcgcgac
     1741 aatctggaga tcagccccaa tttatgggcg ggcgttggct tagtgcgcgg cggtgccgga
     1801 acggcgctgg tgggcgatgg tcctacggtc gccgcgcgaa tcaacgaata cgccgcgctt
     1861 ggcatcgaca gctttgtgct ttcgggctac ccgcatctgg aagaagcgta tcgggttggc
     1921 gagttgctgt tcccacatct ggatgtcgcc attccggaaa ttccccagcc gcagccgctg
     1981 aatccgcaag gcgaagcggt ggcaaatgat tttatccccc gtaaagtcgc gcaaagctaa
     2041 ggaacacgat gatggcaacg ccagtgaaga agtggttatt gcgcgttgcc ccctggtttt
     2101 taccggtggg catcgtggcg gtgtggcaac tggcctcctc ggttggctgg ctgtcgacgc
     2161 gtattttgcc ttcaccggaa ggagtggtga cggcgttctg gacgctctcc gccagcggcg
     2221 aactgtgg
//
LOCUS       DAJVRP010000224         2190 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVRP010000224 DAJVRP010000000
VERSION     DAJVRP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.231"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..699
                     /locus_tag="OQN12_004098"
     CDS             <1..699
                     /locus_tag="OQN12_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656221.1"
                     /translation="ARAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVE
                     NVVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNG
                     NTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAF
                     NGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIP
                     VLDVSDISVKLQEA"
     gene            701..1306
                     /locus_tag="OQN12_004099"
     CDS             701..1306
                     /locus_tag="OQN12_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCS1656222.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1293..>2190
                     /locus_tag="OQN12_004100"
     CDS             1293..>2190
                     /locus_tag="OQN12_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1656223.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          532 a          509 c          611 g          538 t
ORIGIN      
        1 gcccgtgccg gtgtgccggg cagcgatgag gaaacccgtg cggactttga aaaccgccga
       61 cgtaattcag ttgcccgtaa tgcccggaat attctggaag ccatccgggg tgaaatactc
      121 tccacggtag aaaacgtggt ggatgtttac gtcacccata atccgaaaaa aacagaacaa
      181 aaagccggag tcagtcagta tccattaaca cccggttcgc tttatgttgg cgtgtacggc
      241 ggcagcccgg cagatatcgc ggcggccatc tggcgtaagg ctccgccggg tattgatatg
      301 aatggcaaca caacgttcac tgttgcagat aaggagtacg atccgccgta tcctgaatac
      361 gtgatcagct ggcagacact caaacccgtc agtctgcatg tcagtgtgac gctgaaaaaa
      421 agtgactacc tgccttcaga tattacccga caggtacagc aatccgtgct ggacgcattt
      481 aatggtacag atggtggtct gcgggcaaga gttgcctctg ttgtctctgc cgggcgctac
      541 tatgccggca tttacaaaac cgatccggaa catattgata ttctgggcct tactgtgagc
      601 cgtgatggtt cgtcatggac aacggctgtc actttcggga tagatgagat tccggttctg
      661 gatgtgtcgg atatcagtgt gaaactccag gaggcataac gtgcagaatg tggctgccac
      721 tgtgcttgcg cagtatgctg ccagcccccg actcaatgcc ctcattaaca gctttaacgc
      781 agcgctttcc cccgacagtt ttatcagtga tttttatggt cttatctgga acatcgatac
      841 cgcagaaaag tatggtcttg atgtctgggg aaagattgtg ggtgtcagtt gctggctgac
      901 ggtaaaggac gattttaatt acctgggctt cagcgagtcc aggatggaca ccccggtaat
      961 ggatgatccc tgtccgttta atcaggcacc gttttacaac ggaaaatcgg atacccggac
     1021 tgttgacctg tctgatgctg tataccggcg gctgatactg atgaaagcca tgtcgaacat
     1081 tactgactgt tccgttccgg atattaacct gatgctgaga tttatgttcg gaaaaaaacg
     1141 ccgggcttat gttctgaata atggcgggct gaggatgagt tacgtctttg agtccgcgct
     1201 ctcgttggca gaactggcga ttatccagtc gtcgggtgca ctgccatccc cgccgggtgt
     1261 ttatgtttca gtagttttaa aggagtcccg taatgaaggc cagtgataaa ccccgccagc
     1321 tggcggtccc ctttgcgagt accggagata aaaaccgtat cccggacaag gcgacacagc
     1381 agaccagaga gagcggtaat gctgcgtatg attcaggttt tcctccggtg accatgacag
     1441 cggtctcagc gggaggtata ccgccacacg gcaaggattt taacggtctg atgtacgata
     1501 ttaccgcagc aatacggttc gcccaggctg gcggtttgta cacgtataat gccggttttg
     1561 cgggggccat tggtggatat gcaaaaggag ccattctcgc cggagtcgca acaacagcgg
     1621 tctggctgaa taccacggac gataacctga ccgatcctga aggctccgac agtgcgggct
     1681 gggtaaatct tcttgaggat ccgaaaagga tattcctgcg gcagaagaac aatctgtcag
     1741 accttcagaa taaagggacg gcacgggata atcttcaggt ttacagtaaa gagcagtcag
     1801 atcaacgcta tgttcatcgg gaaggcgata aaataaccgg agagctgaaa atccgtggtg
     1861 ttaatgcgct gaggattttc aacgaagctt ttggcctgat ttttcgtcgt tcggaagagt
     1921 gcctgcacct tattcccacc agtgaaggtc agggggaaaa tggcgatatt ggtcccctgc
     1981 gcccgttcac cattaatttg cggacgggtg aaatatccat gtcgcataaa gtgtctgttg
     2041 gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt tcagaacgcc ctggggggga
     2101 attcaattgt tcttggtgat aatgacaccg gatttaaaca gaatggagac ggtattctgg
     2161 atgtttatgc taatagtcag cgtgtattcc
//
LOCUS       DAJVRP010000225         2142 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVRP010000225 DAJVRP010000000
VERSION     DAJVRP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.232"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(134..2002)
                     /gene="kup"
                     /locus_tag="OQN12_004101"
     CDS             complement(134..2002)
                     /gene="kup"
                     /locus_tag="OQN12_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS1656224.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
BASE COUNT          621 a          578 c          509 g          434 t
ORIGIN      
        1 cgcagcttaa tcctgactga gttgggaaaa ccatcgtgac gttacggaaa tgtgatcaac
       61 ctcgaaacgt tttacatggt gattaaccat gaaaacaaaa acgccccctt atgaagaaag
      121 gaggcgtctg gcgttagatt tcgacctgag tacccagttc gataaccctg tttggcggga
      181 tttcaaattg atctggtgca cgcagcgcat tacgttgcag cagcaagtac agcttgccgc
      241 gcagacgcaa ataccacggg cgtttgccga ggatcaacga ctcatgcgac ataaagaagg
      301 aggtttccat catccggcaa cttaatcctt ccagaccgca gcggtggaaa acttcttcca
      361 cgtttggcgt ttctcgccaa ccataacttg ccaccacgcg ccagaaagtg ggcgacagtt
      421 gttcaatctg tacccgacgg acgttatgga catatggcgc gtcttcggtg cgcagagtta
      481 acagaatcac ccgctcatgc aataccttgt tatgtttaag gttatgcatc agcgcaaagg
      541 gaatgacgtt gattgcacgc gacatataca ccgcggtccc gggcacgcga acaggcggtg
      601 atttctccag cgaagcaatc atcgcttcca gagagttacc atgttcatgc atccgccgca
      661 gcaagcggaa acgctcgctc ttccaggtgg tcatcacgat aaacatcaca gtaccgaggc
      721 tcaatggcaa ccagccgccg gagagcagtt tatcgaggtt agcggtgaac aatggaatat
      781 cgacacaaag gaaagcaatc aggatcagcg caacaaaata cttattccag tgccagttct
      841 gacgtgccac ggtagtcgag agaatagacg tcagtaccat ggttccggtc accgcaatcc
      901 cgtacgccgc cgccaggttg ctggagtgct caaagctgac aatcacaatc acgaccgcga
      961 catagagcat ccagttcaca aacggaatat agatttgccc tgactccatt tcggaggtgt
     1021 gaataatgcg catcggcgac aaatatccca gacgtaccgc ctgacgcgtc aatgagaaga
     1081 cgccagagat aaccgcctgc gaggcaatta ccgtcgccag tgcggcgatg atcagcagtg
     1141 ggatcagcgc ccagtccggt gccaacagga agaacgggtt cttaatcgct tccgggttct
     1201 ttaacaacaa cgctccctga ccgaagtaat taagggtcaa ggaaggtaat acgacggtga
     1261 accacgccag gcgaataggg aacttaccaa agtgccccat atcagcatac agcacctcgc
     1321 cccccgtaat cgacagcacc actgccccta atgcaataaa agaaaccgtt ttgtattcaa
     1381 ggaagaaatg caccgcccac atcggattca gcgcatgcag cacttccggg ttagcaataa
     1441 tgctacgtaa ccccagtcct gccagaatca aaaaccaggt cagcatgatc ggcgcaaaca
     1501 gcttaccgac catagccgtg ccatgctttt gaatcataaa taataaagtg agaacgataa
     1561 ttgagagggg aactatccag gtatccagct gcggggcgac gatttccaga ccttcaatgg
     1621 ctgacatcac cgaaatagcg ggtgttatga cgacttcacc atagaaaaag ctgccgccga
     1681 ttagccccat aatcaccagc attgatgtgg ttcgcgccga cgtattacgc ccggcaagcg
     1741 acatcaacgt caggatcccc ccctcaccgg cgttatctgc ccgcatcacg aaggtgagat
     1801 atttaatgga aaccacaaag attagcagcc agaagatcag cgataaaaag ccaaacacgg
     1861 catcgcgttc aacgccaaaa ccaaactggc cggacaaaca ttcacgaagt gtatataacg
     1921 ggctggtacc aatatcgccg tagacaactc caatcgccgc gagggtaatc gcgggcaatg
     1981 attgcttatt atcagtgctc atagactagt ctttcgttga aatatgaaat gtgtgcttag
     2041 tcccttggcc cacaaaaaag cgcacagtat gcacgattaa cggcaaaatc gtactcctaa
     2101 atgcggccac attaacctgg ctcaaagaaa aagccccccc tt
//
LOCUS       DAJVRP010000226         2124 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVRP010000226 DAJVRP010000000
VERSION     DAJVRP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.233"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            42..221
                     /locus_tag="OQN12_004102"
     CDS             42..221
                     /locus_tag="OQN12_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656225.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            273..467
                     /locus_tag="OQN12_004103"
     CDS             273..467
                     /locus_tag="OQN12_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656226.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1266..1602)
                     /locus_tag="OQN12_004104"
                     /pseudo
     CDS             complement(1266..1602)
                     /locus_tag="OQN12_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          682 a          437 c          436 g          569 t
ORIGIN      
        1 agcgtaaggc actacagcgc attgctttac gagtaataac aatggattta tcaccagaag
       61 acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag agaagccatt atcgaacagc
      121 aaacgggcat gagcgccata gacccattgc gatacaaacg ggcctcatct cctttagttg
      181 acgacaattc caatgcggat aaaacacgtc caccacactg attacatttt tcattagcac
      241 agtggtgtga tgcagcatac gaggaaaacc ggatgaatga gaacaaaata aaaaggctgg
      301 agcaactgtt acaggcacgc caacgggagt ttgccactaa atggggacgt gccgcgctgc
      361 cacatgaacg cctaacctat tttccacttg aagacctcat taaaaataat ggcaaaaaaa
      421 tcaggtctga taagaaacac ttcttagaat gcaatgaaga caaataacaa caatctgcat
      481 attgccacca accacagatg tggcatcgtt atcagcgcac ttgattttct ttatattcac
      541 tgacgagctt gccggggcac ctttgtttgc cggtcattct ggtatcatgc agatatttac
      601 ggcgtacaag cccactagca tgacattgta ataaggcctg agcctaacgc tttaatgtgt
      661 gtcatacact cagtggcgta atcgttgaca atatggtgca tgtaatcgta agcgtccagc
      721 gaaatccatc tggtcataat ctgaagcatc cgacaaagtg gtgtccacca aataagtgat
      781 ctgcgcccaa tatcctgagc cggaactttg acaaaggcca acgaaaaatg ggagcggatg
      841 ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg ccagttctcc gatctgttta
      901 accgggaaat tatctcctac agcttgtcag aaaggtcggt gatggagcat cgttaatacg
      961 atgctatacg atgcattcac agtgcccggc cagaggatgc cccgtcgctg catatggatc
     1021 agggttggca atatcgaatc gcaggctata ggcaaagtta aagccccatg gagtagcaca
     1081 aaatatgccg cacaaaggaa acggtctgaa taacgcagtg atgaagaact tcttcagcac
     1141 actgctaaaa cgcatagtga tcgagcgctg attctggcga acaactgaac taatacatca
     1201 gaatatgcat tatgttaaat aaatatgaaa agatggttta aataccccgt tacttttgac
     1261 ttacactata cggtatcgca tcgtttaata ttcgcaccgg ccagattttt atttctatta
     1321 gttgtcacaa tactgaatgc gtacgaccac agtattctgg ctcctgtgtg gttatgcttt
     1381 aattctgcgt tccgggcaga taagcagttg cttgcaggaa tccttcttgt gttaatgtca
     1441 gttccccttt ttaccagtgc tgatttccac attccgtcca acagagctta tagcctttcc
     1501 ctggattata gcattgtccg gctgaagttc tttttgaata ataatagaag cactgctggc
     1561 agatccagtc cgtttttcat aacccactgt actgataacc ataatctaat cagtagaaat
     1621 tgagtcgaaa ataagcacta ctccatacag gataattaga ggtcagtttg attattcaca
     1681 attcatcatc agcattttct atttctgacg aaatcaacat gaaaataacc atatatgata
     1741 attattataa taacggcttt aattggaata catatattac aacgtattat atataattgg
     1801 tattctggga actatattct caaaatatag tagaaacgag gtatgtttct ggtggaaagg
     1861 acagtgggat taaaaagtaa gaattgataa aaaaaacgcc agcaacacat agtgccggcg
     1921 gagggaatac cccatggaga aaatgtgatg cctagagcat catgaatata taattaaaaa
     1981 tagttagcgt tgtcaatact ttcacaaaaa taattttcgt agtatagaaa gatattttta
     2041 tgcatgacct acctgaattt gctccgggta gaggttataa ataaaaattg aatcacgaca
     2101 aacacaatat tcatagtatg gcga
//
LOCUS       DAJVRP010000227         2052 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVRP010000227 DAJVRP010000000
VERSION     DAJVRP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.234"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(61..330)
                     /locus_tag="OQN12_004105"
     CDS             complement(61..330)
                     /locus_tag="OQN12_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1656227.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            387..1055
                     /locus_tag="OQN12_004106"
     CDS             387..1055
                     /locus_tag="OQN12_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1656228.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1371..>2052
                     /locus_tag="OQN12_004107"
     CDS             1371..>2052
                     /locus_tag="OQN12_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1656229.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLL"
BASE COUNT          616 a          449 c          382 g          605 t
ORIGIN      
        1 gtttaccagc caaccgcagc acgtcctgca tacgtcgtgt ctgcggtttt tctttttcgc
       61 ttacattgtg tctggttctt ccggccactc aatatcaggt gcagttgatg tatcaacacg
      121 gttcagcaac acccgatact tcttccaggc ttccagcaac gagttttctt cctccgttgc
      181 gatctccaga tctacagcat cctgaagtgg cgcaatatgc tcactgaatc cctggatgta
      241 gaactgtgtg gtgacggtct tccagccatt cggctcctgc tgtatcgaag cataccaggc
      301 tatttcaata tcgctatgct gcggcagcat ttaacccctt gtaattcatc gccataattg
      361 atttaattca caaataaaac tataatatgg tgaaattaat gaaaaaaaac acagatgatg
      421 gggctaaaat ttacacacca cttaccctaa agctttatga ctggtgggtt ttgggagtat
      481 caaatcggct tgcatgggga tgtcctacaa aggaacacct tcttccacac tttctggaac
      541 atttaggtaa caaccatctg gatattggtg ttggaactgg gttttacctt actcacgtac
      601 ctgagagtag tctgatatct ttaatggatt tgaacgaagc tagcctgaac gcggcatcta
      661 caagggctgg ggaatcaaaa attaaacata aaattagcca tgatgttttt gaaccttatc
      721 ctgcggcgtt acatggtcaa tttgattcca tttccatgtt ttaccttctt cactgcctgc
      781 ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc ggcgcaggcc ttaactgatg
      841 atggaactct atacggagcc acaattcttg gcgatggagt tgtgcacaat agcttcggtc
      901 aaaaactgat gcgcatttac aatcagaaag gcatcttttc aaacacaaaa gattccgaag
      961 aaggcttaac acatatactc tcagagcatt tcgagaatgt taaaaccaag gttcaaggta
     1021 ctgtagtaat gttttccgct tcagggaaaa aatagcatcc aaccgcagca cgttcttgct
     1081 taagacgtgc tacggtttcc tttacgattt ggggttgcga ctttacctct attgataatg
     1141 cattccggca gaacgttcaa atatgaacgt acgatattta actaaccgaa aaacaaaata
     1201 tagcacaggc gagacattta ccatctaaga aaatagtatc gtttttttac agttaaatca
     1261 acatcacttc cttaaaatga aaacaataaa tttcaatcct gaaacatttt atttcagctt
     1321 attattcccc agtatcacat tttatcagga tatccttctg cgaggttatt atgcttcctg
     1381 taaataatcc ccccctatcc actggaaacg tctcttttta cagaactaca tcaatcgaca
     1441 atgttcacaa taattatctc tccgaatggg ttgaatggac taaaaacagc atttccggag
     1501 aaaacaggga aactgctttt acccggctcc aattatgtct ggagaacagt gaaacatcgt
     1561 tggacttatc ttgtttaggt ctcagatctc taccacgatt gcctgacaat cttgatgaaa
     1621 ttaatgtaag caataaccaa ctatcaatgc tccccgagct accaagggca ttgaaagagc
     1681 tgaatgcaag cagtaatcaa ttatctgcac ttcctgaatt accagtgtcg ctggaatata
     1741 taaatgtgag tgataaccat ttgttcgcac ttcctgaatt acctgcgtca ctagaatata
     1801 ttaatgtaag tgacaatcac ctgtctgtac ttccgaggtt accaatgtca ttggaattac
     1861 ttgatgcagc cagaaatgct ttggaagtaa taccagattt tccagaaaga gatgatcata
     1921 ttataagaat attctggctt aatcagaacc ggatcacggc aattccggaa agcatacttg
     1981 gcctcagttc tgatagcgtt gtcaatctta gagaaaatca actatctccc agaataatgc
     2041 aaactttgtt ac
//
LOCUS       DAJVRP010000228         2045 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVRP010000228 DAJVRP010000000
VERSION     DAJVRP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.235"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            6..224
                     /locus_tag="OQN12_004108"
     CDS             6..224
                     /locus_tag="OQN12_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCS1656230.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OQN12_004109"
     CDS             226..591
                     /locus_tag="OQN12_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1656231.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="OQN12_004110"
     CDS             588..1253
                     /locus_tag="OQN12_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656232.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OQN12_004111"
     CDS             1253..1618
                     /locus_tag="OQN12_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS1656233.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          559 a          424 c          542 g          520 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttac
//
LOCUS       DAJVRP010000229         2035 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVRP010000229 DAJVRP010000000
VERSION     DAJVRP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.236"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..363)
                     /locus_tag="OQN12_004112"
     CDS             complement(<1..363)
                     /locus_tag="OQN12_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1656234.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="OQN12_004113"
     CDS             complement(430..1644)
                     /locus_tag="OQN12_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCS1656235.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          493 c          413 g          495 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggttacca gaatg
//
LOCUS       DAJVRP010000230         2035 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVRP010000230 DAJVRP010000000
VERSION     DAJVRP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.237"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>2035
                     /gene="sat"
                     /locus_tag="OQN12_004114"
     CDS             <1..>2035
                     /gene="sat"
                     /locus_tag="OQN12_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCS1656236.1"
                     /translation="SYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHG
                     IASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQ
                     EKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKES
                     IVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGK
                     VILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVL
                     AINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQG
                     HATEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDW
                     KTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGF
                     DFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVI
                     AQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASV
                     TGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNN
                     AIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          746 a          359 c          401 g          529 t
ORIGIN      
        1 tcatacggca tgatgattta taaaaatgat aaaacatttc gtaatcttga aatattcgga
       61 gacagcggct ctggagcata cttatatgat aacaaactag aaaaatgggt attagtcgga
      121 acaacccatg gtattgccag cgttaatggt gaccaactga catggataac aaaatacaat
      181 gataaactgg ttagtgagtt aaaagatacc tatagtcata aaataaatct gaatggcaat
      241 aatgtaacca ttaaaaacac agatataaca ttacaccaaa acaatgcaga taccactggt
      301 actcaagaaa aaataactaa agacaaagat attgtgttca caaatggggg aaatgttctg
      361 tttaaggata atttggattt tggtagcggt ggtattatct ttgacgaagg ccatgaatat
      421 aacataaacg gtcagggatt tacatttaaa ggagcaggaa ttgatatcgg aaaagaaagc
      481 attgtaaact ggaatgcatt gtattccagt gatgatgttt tacacaaaat aggccccggt
      541 actctgaatg ttcaaaaaaa acagggggca aatataaaga taggtgaagg aaatgttatt
      601 cttaatgaag aaggaacatt taacaatata taccttgcaa gcggaaatgg taaggtaata
      661 ctaaataaag ataattccct tggcaatgat caatatgcgg ggatattttt tactaaacgt
      721 ggtggcacgc tagatttaaa tggacacaat cagactttta ctagaattgc cgccactgac
      781 gatggaacaa caataactaa ctcagataca acgaaagaag ccgttctggc aatcaataac
      841 gaagactcct acatatatca tgggaacata aatggcaata taaaactaac gcacaatatt
      901 aattctcagg ataagaaaac taatgcaaaa ttaattctgg atggtagtgt caacacaaaa
      961 aatgatgttg aagtcagtaa tgccagtctt accatgcaag gccatgcaac agagcatgca
     1021 atattcagaa gcacagcgag tcattgctcc ctggtatttc tttgtggaac ggactgggtc
     1081 accgttttga aagaaacaga gagttcatat aataaaaagt tcaattctga ttacaaaagt
     1141 aataatcagc agacctcatt tgatcagcct gactggaaaa ccggggtgtt taaatttgat
     1201 acattacacc tgaacaatgc tgacttttca atatcacgca atgccaatgt tgaaggaaat
     1261 atatcagcaa ataaatcagc tatcacaatc ggcgataaaa atgcttacat tgataatctt
     1321 gcagggaaaa atattactaa taatggtttt gacttcaaac aaactatcag tactaatcta
     1381 tccataggag aaactaaatt tacaggtggc atcactgcac ataacagcca aatagccata
     1441 ggtgatcaag ctgtagttac acttaatggt gcaacctttc tgaataatac tcctataagt
     1501 atagataaag gagcaaaagt tatagcacaa aattccatgt tcacaacaaa aggtattgat
     1561 atctccggtg aactgactat gatgggaatc cctgaacaga atagtaaaac tgtaacgccg
     1621 ggtctccact acgctgctga tggattcagg ctgagtggtg gaaatgcaaa tttcattgcc
     1681 agaaatatgg catctgtcac cggaaatatt tatgctgatg atgcagcaac cattactctg
     1741 ggacagcctg aaactgaaac accgactata tcgtctgctt atcaggcatg ggcagagact
     1801 cttttgtatg gctttgatac cgcctatcga ggcgcaataa cagcccccaa agctacagtt
     1861 agcatgaata atgcgatctg gcatctaaat agccagtcat caattaatcg tctagaaaca
     1921 aaagacagta tggtgcgttt tactggtgac aatgggaagt ttacaaccct tacagtggac
     1981 aaccttacta tagatgacag tgcatttgtg ctgcgtgcaa atctggccca agcag
//
LOCUS       DAJVRP010000231         1937 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVRP010000231 DAJVRP010000000
VERSION     DAJVRP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.238"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..546)
                     /locus_tag="OQN12_004115"
     CDS             complement(<1..546)
                     /locus_tag="OQN12_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656237.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1201..>1937)
                     /locus_tag="OQN12_004116"
     CDS             complement(1201..>1937)
                     /locus_tag="OQN12_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656238.1"
                     /translation="NGAFNPAKDAPSTTVIKGASVTVTPVLNPETNKTGILCSAATHV
                     SDLDMMGGWTEGALIQTVCDMQVQYCRQMFAAVVDVLKDTSDLQIIEAAPLSGKPSDQ
                     AEDLLDTLALNLPVELGNTLSDYAVLVPEKLEAILDRAAQRAGHEGISELLGCTVCGY
                     AGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVVDVLATGSVK
                     VKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          545 a          441 c          417 g          534 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggaacctag gtatcgaggg tatcgaagat aatcagccgc taaccatttg tttccttaaa
      841 ggatgatgaa tccttgtggt tacagttatg gttacacatt aatttatctc tggtcaagtc
      901 ccgaagtcct aactttaaga cagtggcccc aatcgtccag acttgaaagg ggcctatggg
      961 ggaagtcgct gacctttatc tattaaatac ctacagagat ttttgtgaga ttttttaatc
     1021 caaggtcata acctactgcc cccacaggcc gcaaagtgaa gacctgtaag caccagagca
     1081 atagcgagta tctatcactc cctgtagagt atctggtaga ccaagaggac acagaggacg
     1141 accatataca actcctgtaa gctcatggat gtactcctta tgttcactca gggtttatag
     1201 ttactctgcg gcagtaaaac gaataacatg gaccaacggg aaagaggcgt cttcctcaac
     1261 gtcaaactca cctgctttca ccttaacgga accagtagcc aacacatcga ccacactgat
     1321 taactcaagg tcatacccag cagtgttgct atttcgggtc actttgacat ctaccgtctt
     1381 agcgtcttta gttgacctga aagagataga agcgaaccgc ttaggcagga gatagacacc
     1441 tgtatcatcg cccgcatagc cgcatacagt gcagcccaat agctcactaa taccttcatg
     1501 acctgcgcgt tgagcggctc ggtccaagat tgcctcaagt ttctcaggga ccaatacagc
     1561 gtaatcgctc agtgtatttc ccaactcgac cggaagattc aacgctaatg tatccagaag
     1621 atcttcagct tgatcagacg gtttaccact tagtggggct gcctcaatga tttgaaggtc
     1681 tgacgtatct ttcaggacat cgaccacagc ggcgaacatc tggcggcagt attgaacctg
     1741 catatcgcat acagtctgga ttaatgcccc ctcggtccaa ccgcccatca tatcgaggtc
     1801 agacacatga gtagcagcag aacacaggat gcccgtctta ttggtctcag ggttcaacac
     1861 tggggtgaca gttacgcttg cccctttgat gactgtggta gacggagcat ccttagctgg
     1921 attaaacgcg ccattat
//
LOCUS       DAJVRP010000232         1926 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVRP010000232 DAJVRP010000000
VERSION     DAJVRP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.239"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OQN12_004117"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="OQN12_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCS1656239.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1926
                     /locus_tag="OQN12_004118"
     CDS             1494..>1926
                     /locus_tag="OQN12_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCS1656240.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHAD"
BASE COUNT          548 a          335 c          464 g          579 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgatt
//
LOCUS       DAJVRP010000233         1876 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVRP010000233 DAJVRP010000000
VERSION     DAJVRP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.240"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..224)
                     /locus_tag="OQN12_004119"
     CDS             complement(<1..224)
                     /locus_tag="OQN12_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1656241.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADA"
     gene            complement(221..754)
                     /locus_tag="OQN12_004120"
     CDS             complement(221..754)
                     /locus_tag="OQN12_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1656242.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(820..1449)
                     /locus_tag="OQN12_004121"
     CDS             complement(820..1449)
                     /locus_tag="OQN12_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656243.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1453..1668)
                     /locus_tag="OQN12_004122"
     CDS             complement(1453..1668)
                     /locus_tag="OQN12_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1656244.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          482 a          499 c          407 g          488 t
ORIGIN      
        1 gcatcagcta actccttcgt gtatttagca tcgagtgcat cagcatcacg ctggcgctgc
       61 tgcatgtcag taatggttgc gttcgccagc ttcagttctc cggcgttttt gtcgcgctgc
      121 tctttgtagg taatggcgtt atcacggtaa tgattaacag cccatgacag gcagacgatg
      181 atgcagataa ccagagcgga gataatcgcg gtgactctgc tcattgatct atcccccaac
      241 aggctaatgc gctttcctgg tcacgacgaa taacctgtcc atagcagtta tttgaacgta
      301 tgcggcaatc gcgcccaaca tcttttatcc accagcgaat cgcctcgcat gcgcccttac
      361 gatcaccggc attcagccgc ttataaaacg tcgacgggaa acacttaccg gggccaatgt
      421 tataggggca aaatgacgct atacccgctt tctggggttc ggtcagaggt actttaatat
      481 tgcgctccac ccatgccagc gccttatcac gttcaatagc gttaacctgg tcacattttt
      541 ccttcgacag cttcattccc ggtatgacag gcttaccatc caccattgtg gcaccacgac
      601 agatggtcca tatgccagaa ccatcacggt atgccgttgt gtgattaccc tctttttcgt
      661 tcagaaactg gtcaagtatc tggggagcag acgcgcctgc ggcaatcagc gccagaacag
      721 cagccgacag gccgtatttg atttttgcgc tcatggatat ttatcaggat ttatcggcaa
      781 caaataacga gccaacttat atacgccttt taagataagt cagccccggc tggatccagt
      841 catcaggctc tttcttaaag ggggagtatt gaaatcacga agaagagcct cccgcaccgc
      901 agcatccatg tctgcaccac tggcaagtgc ttcaatctct gccgccacct gcaaataccc
      961 catgcaacgg ccaatgcgtt tcataactct ggtgttttcg tctttcaggc atctgatagc
     1021 gagattaata attttcaggc agtgatgatg cgatgcccgg acaggttttc cgttctcata
     1081 aacgacgaac tcaccctctt cacaggaaac cctgaaaggc cgatggggtt cctggtcttt
     1141 atccggtgca gacgcaataa aatcactttt atgctcttcc tctccggcac cgttaaccgt
     1201 aatgacgtac tcaggccgca ggtaagccgc ctctttttct gacaatgcgg ccggtaaaaa
     1261 cttttcctgc aaaaacttca taaaagtctc gtgagcgact ttgtggcagt aatcgtaaat
     1321 cgccgcataa tgctcatcgc ggcgtttgtt ttcatcttca gaactcacca acgccgacaa
     1381 ttttttatta agctcagtga tttcattttc caggtgattg aatcgctgat tcatttcttc
     1441 atgcttcatt atttactctc cccgtgcagc cttacgcttg tcttctctta ttttgaaata
     1501 cagattcgtc agataagtca gaagccccag aagcagactt cccagcacac caatcgcagc
     1561 ccactgtgat ggcctgacct gatccaacca ctgcaaaaac cagtagccgg cactgccggc
     1621 ggaggtgccg taggcaatgc ccgttgaaat tttgtccatg gatttcatag cctcacctcc
     1681 gcaaataacg gatggcgtag ttttacactg agaaatgaaa gggatttgaa aagaaaaaaa
     1741 cgcaaaagcg ggcgaaacga tatatacagt aaggaaagca ctctatccaa caaaccaccc
     1801 acagttaatc ggaataaaag cagagtgctt atgaatgatc gcacgcccga aggttagtat
     1861 ttctgcacag caattt
//
LOCUS       DAJVRP010000234         1812 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVRP010000234 DAJVRP010000000
VERSION     DAJVRP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.241"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            177..1202
                     /locus_tag="OQN12_004123"
     CDS             177..1202
                     /locus_tag="OQN12_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1656245.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADANGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1270..1812
                     /locus_tag="OQN12_004124"
                     /pseudo
     CDS             1270..1812
                     /locus_tag="OQN12_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          422 a          421 c          462 g          507 t
ORIGIN      
        1 ttcgcaccat tgcggggcgc tgtttttatt atttcctgtc tacactcagc ttattgcgac
       61 gcgaaatcgt gcgcaaaagc tggcactact tttgcttata agaggatggc cacagacagg
      121 taaaagacgt ttcccaaacg tcctataacg ataattttcg cctcacagga agcattatga
      181 aaaagatgat gatagccaca ctggctgccg ccagcgtgct gcttgccgtt gcaaatcagg
      241 cgcatgctgg cgcgacgctt gatgccgttc agaaaaaagg ttttgtgcaa tgcgggatca
      301 gtgatggatt acctaggttc tcttatgccg atgcaaacgg taagttttca ggtattgatg
      361 ttgatgtttg tcgtggtgtt gccgctgctg tatttggtga cgacacgaaa gtgaaatata
      421 ccccactcac agcaaaagaa cgcttcaccg ctttacagtc aggggaagtg gatttgctct
      481 cccgtaatac gacctggact tcatctcgcg atgccgggat gggaatggca tttaccggcg
      541 tcacttatta cgacggcatt ggcttcctga cgcacgataa agcggggcta aaaagcgcga
      601 aagaactgga tggcgctacc gtctgtattc aggcgggtac tgataccgaa ctcaacgtcg
      661 ccgactattt caaggcaaac aatatgaagt acacaccagt gactttcgat cgctctgacg
      721 aatcagcgaa ggcactggaa tctggtcgct gcgatacgct agcttcggat cagtcgcaac
      781 tgtatgcctt gcgcatcaaa ttaagcaacc cggctgaatg gattgtctta ccggaggtta
      841 tctctaaaga accgcttggg ccggtagttc gtcgtggcga tgatgaatgg ttctcgattg
      901 tacgctggac gcttttcgcc atgctaaatg ctgaagagat gggcatcaat tcccagaacg
      961 tcgatgaaaa agcggctaat ccagcaacgc ctgatatggc acatctgttg ggtaaagaag
     1021 gcgattacgg caaggatctg aagttggata ataaatgggc ctataacatc atcaaacagg
     1081 tgggtaacta ctcggaaatt tttgagcgta acgtaggttc agaaagcccg ctgaaaatta
     1141 aacgtgggca aaataatctc tggaataacg gcggtattca gtacgcgccg cccgtgcgtt
     1201 aagtcggtgt gaggtaacga gcgtcgctac ggcggcgctc tcttcagcgt tatgctttcc
     1261 gaggtttcta tgtctcatcg ccgctcaacc gttaaaggct cactctcctt tgccaaccct
     1321 acggttcgtg cctggttatt ccagatcctt gccgttgttg ctgttgtcgg cattgttggt
     1381 tggttatttc aaaacactgt aacgaatctc aataatcgtg gcattacttc aggttttgcc
     1441 tttctggatc gcggcgctgg cttcggtatt gtccagcatt tgatcgatta ccagcagggc
     1501 gacacctacg gacgcgtttt tattgtcggc ttactcaata cgctactggt ttctgcattg
     1561 tgtattgtgt tcgcttctgt tttgggcttc tttatcggtc tggcgagact ttcggataac
     1621 tggctgctac gaaagctttc cacaatttat attgagatct tccgtaatat tgcaaatctt
     1681 cttctggtac tttgccgtgt tgcgcaattt gcccggacca cgccaagctg tgagcgcgtt
     1741 tgatctggcc tttttgagca atcgtgggct ttatattccg tcaccgcagc tgggagacgg
     1801 atttattgcg tt
//
LOCUS       DAJVRP010000235         1810 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVRP010000235 DAJVRP010000000
VERSION     DAJVRP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.242"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..570
                     /locus_tag="OQN12_004125"
     CDS             <1..570
                     /locus_tag="OQN12_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1656246.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="OQN12_004126"
     CDS             571..936
                     /locus_tag="OQN12_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1656247.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="OQN12_004127"
     CDS             933..1616
                     /locus_tag="OQN12_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1656248.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          504 a          384 c          500 g          422 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgcatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgtgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc
//
LOCUS       DAJVRP010000236         1794 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVRP010000236 DAJVRP010000000
VERSION     DAJVRP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.243"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(110..577)
                     /locus_tag="OQN12_004128"
     CDS             complement(110..577)
                     /locus_tag="OQN12_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1656249.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OQN12_004129"
     CDS             complement(643..882)
                     /locus_tag="OQN12_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCS1656250.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OQN12_004130"
     CDS             complement(1017..1622)
                     /locus_tag="OQN12_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1656251.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
     gene            complement(1737..>1794)
                     /locus_tag="OQN12_004131"
     CDS             complement(1737..>1794)
                     /locus_tag="OQN12_004131"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656252.1"
                     /translation="VELHDKVIGHYLNIKHYQ"
BASE COUNT          569 a          397 c          317 g          511 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tggttttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata ttttttggat
      961 gtggctataa ttatccattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagcaatt aggtaatgac tccaacttac
     1741 tgatagtgtt ttatgttcag ataatgcccg atgaccttgt catgcagctc cacc
//
LOCUS       DAJVRP010000237         1726 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVRP010000237 DAJVRP010000000
VERSION     DAJVRP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.244"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..1241
                     /gene="ccp"
                     /locus_tag="OQN12_004132"
     CDS             <1..1241
                     /gene="ccp"
                     /locus_tag="OQN12_004132"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1656253.1"
                     /translation="LREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEA
                     VRAALLADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQR
                     AEYYASNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTL
                     NAGGVDGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEM
                     ASKSWDEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLR
                     GDENALTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRM
                     NVTKEERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDD
                     IVAFLHSLNGVYTPYMQDKQ"
     gene            1452..>1726
                     /locus_tag="OQN12_004133"
     CDS             1452..>1726
                     /locus_tag="OQN12_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1656254.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYP"
BASE COUNT          479 a          355 c          435 g          457 t
ORIGIN      
        1 ttctccgcga aaaaggatgc gactattgcc acacgccttc ggcagaatta cccgcctatt
       61 attatattcc tggcgcgaaa cagttgatgg attacgacat taagcttgga tataaatctt
      121 ttaacctcga ggccgtgcgt gcggcactgc tggctgataa acccgtttcg caaagcgatc
      181 tgaataagat tgaatgggtg atgcagtatg aaactatgcc accaacgcgt tataccgcgc
      241 tacactgggc gggtaaggtg agtgatgaag agcgggcgga aatactggcc tggattgcaa
      301 aacagcgcgc ggaatattac gccagcaatg atactgctcc ggagcatcgc aatgaaccgg
      361 tgcagcccat cccgcaaaaa ctgcctaccg atgcgcaaaa agtggcgttg ggctttgcgc
      421 tgtatcacga tccccgttta tcggctgata gcaccatttc atgcgctcat tgccatacgt
      481 tgaatgcggg gggcgtcgat ggcagaaaaa catcgattgg tgttggtggc gcagttgggc
      541 cgattaatgc gccgacggta tttaactcag tatttaacgt tgagcagttc tgggatggtc
      601 gtgcggcaac attgcaggat caggcaggtg gaccgccgtt gaacccgatt gaaatggcgt
      661 cgaaatcctg ggacgaaatt attgctaagc tggaaaaaga tccgcagctt aaagcgcagt
      721 tcctcgaagt ttatccgcaa ggtttcagtg gcgaaaatat tactgatgcc attgctgaat
      781 ttgagaaaac attaattacg ccggattccc catttgataa atggttgcgt ggagatgaaa
      841 atgcgctgac ggcgcaacag aaaaaaggct atcaattatt taaagataat aaatgtgcaa
      901 cttgtcatgg tggtattatt ctcggcggac gttcctttga accgttgggg ctgaaaaaag
      961 actttaactt tggggaaatt acggcggcgg atattggtcg tatgaatgtg actaaagaag
     1021 agcgtgataa attgcgtcag aaagtacccg gtttacgtaa cgttgcttta acggcaccgt
     1081 acttccatcg cggtgacgtg ccgacgctgg acggggcggt gaaactgatg ctgcgctatc
     1141 aggtaggcaa agagctgccg caggaggatg tggatgatat cgtagctttc ctgcacagtc
     1201 tgaacggggt gtacacgccg tatatgcagg ataaacaata attaatttga tcgcccgaac
     1261 aggaatgttt gggcgatttt tattacgata ataaagtctg tttttaatat tatcatgtta
     1321 aatgtttata ttataaaaag tcgtttttct gcttaggatt ttgttattta aattaagcct
     1381 gtaatgcctt gcttccattg cggataaatc ctactttttt attgccttca aataaattta
     1441 aggagttcga aatggaccag aagctgttaa cggatttccg ctcagaacta ctcgattcac
     1501 gttttggcgc aaaggccatt tctactatcg cggagtcaaa acgatttccg ctgcacgaaa
     1561 tgcgcgatga tgtcgcattc cagattatca atgatgaatt atatcttgat ggcaacgctc
     1621 gtcagaacct agccactttc tgccagacct gggacgacga aaacgtccat aaattgatgg
     1681 atttgtcgat caataaaaac tggatcgaca aagaagaata tccgca
//
LOCUS       DAJVRP010000238         1638 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVRP010000238 DAJVRP010000000
VERSION     DAJVRP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.245"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..285
                     /locus_tag="OQN12_004134"
     CDS             <1..285
                     /locus_tag="OQN12_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1656255.1"
                     /translation="SQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEA
                     TNVIAPLQDAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(576..1577)
                     /locus_tag="OQN12_004135"
     CDS             complement(576..1577)
                     /locus_tag="OQN12_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1656256.1"
                     /translation="MHKSNCFDTARLVAAMMVLISHHYALSGQLEPYIFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSVLTYFLFGWMLNNFSTE
                     YFSHDIIRKTISSILMSQAPDADITSHLIHPGINGSLWTLPLEFMCYIITGVAVALLK
                     SGKTFIVILLIFVSLSLVESVSENKDVIFSIPVWLYPLRGMAFFFGATMAMYEKSWNV
                     SNVKISVISLLAMYAYASYGKGIDYTMACYILVSFSTIAICTSFGDPLVKGRFDYSYG
                     VYIYAFPVQQVIINTLHIGFYPSMLLSAIVVLFLAHLSWNLIEKRFLTRSSPKLSLN"
BASE COUNT          549 a          324 c          319 g          446 t
ORIGIN      
        1 tcacagagtg taacaaaaat gattacctgt ggtgagaacg gtcaccctgt cattgttgac
       61 cgtccttctc caacaccaga acaattagcc ttaataaatg atgaaaagaa atctgcactg
      121 atagcagagg caacgaatgt aatagcgccg cttcaggatg cggttgattt aggtatggca
      181 acagatgatg aaacgaaact gttactggca tggaaaaaat atcgcgtact gcttatgcgt
      241 gttaatgtag taaaacccga gtggcctatg catccaaata aatagacttg ttaatctagg
      301 gtaattgttg gaataatacg catttttcaa caggtccaga taacctggat ataatgctca
      361 cttctacaat ggcctcaact tattttattt aattgatatt acggaagtta actcatacat
      421 aaacaatatg actcagtact gtactaaata gctgcgtgga aaagaactcc accttcgctc
      481 gtccttgcag gacgaggcat cagaacggtg tccactgcca ggcagtgtta tatctggctt
      541 tgatttattc aggggctcga tggccccttt attattcaat tcagggataa ttttggcgag
      601 cttcgtgtaa ggaatctttt ttcaattaga ttccaagaaa gatgagccaa gaaaagaacg
      661 acaatggcac ttaacaacat cgaagggtaa aagccaatat gcaatgtatt tataataacc
      721 tgctgaacgg ggaatgcata aatgtataca ccataagagt aatcaaacct acccttaacc
      781 agtggatctc caaaagatgt gcatatagct attgttgaga aagaaaccag aatatagcaa
      841 gccattgtat agtcaatgcc tttgccatag ctcgcgtatg cgtacatagc aagcaaagaa
      901 atcacggaaa ttttcacatt acttacattc caagatttct catacatcgc catagtggca
      961 ccaaaaaaga aagccatccc ccgcaaagga tacagccata ctggaatgga gaatattaca
     1021 tccttgttct cagatacact ttcaactaat gaaagtgaaa caaaaatcaa tagtataact
     1081 atgaacgttt ttccgctctt tagtagagca acagccactc cagttattat gtaacacata
     1141 aactccagtg gtagtgtcca caagctgccg ttaataccgg gatgaattaa atgtgatgta
     1201 atgtcggcat ctggagcctg actcattaaa atagagctta tggtcttccg gataatgtca
     1261 tgactaaaat attctgtact gaagttgtta agcatccacc caaataaaaa atacgtcagt
     1321 acagagcaag ggacaagagc agggaatatt cttcttgctc ttttagccat gaaatcaata
     1381 aaggaatcac ttctgattgc tgattttgaa atcaggtatc ctgatatgga aaagaatatg
     1441 ataacagcaa tgccgccagc actttcaaag ccgaatatat acggctcgag ttgtccagag
     1501 agcgcgtagt ggtgactgat tagaaccatc atagctgcca caagccttgc cgtatcaaag
     1561 cagttgctct tatgcattaa aataaaatcc ctaagttttt gtgaggaaaa tatcagatct
     1621 ttatgtcata gtccattt
//
LOCUS       DAJVRP010000239         1606 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVRP010000239 DAJVRP010000000
VERSION     DAJVRP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.246"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            63..251
                     /locus_tag="OQN12_004136"
     CDS             63..251
                     /locus_tag="OQN12_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656257.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            261..1459
                     /locus_tag="OQN12_004137"
     CDS             join(261..1077,1077..1459)
                     /locus_tag="OQN12_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1656258.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTQWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDIS
                     LLRYMPA"
BASE COUNT          378 a          408 c          501 g          319 t
ORIGIN      
        1 gccgcacgct ccgcagaata tgtatgctct gaggacctgt tttattaagg actcatcctc
       61 ttatgttgac ccgcaaaagt atagataccg ttctgctctc tgttggtgct gagaagctct
      121 cacaacgtga atgggactgg atgaagatgc tcaaacccat ggacccacca cctgcgatgg
      181 tcgcggcctc catcctggag cgccgtggcg acacagcagc ccttacccgg ctacaggata
      241 ccgggggtta atccgtcctc atgttgtccg cttttactcc ccgcccgctg aaacgtctgt
      301 tcacggccaa ccagtgctgg acatccttcc tggatgcggg cggtctgcgc gatatcgagg
      361 ttgaggctgt cacaaaaatg ctggcctgcg gcacgcggat actgggcgtg aaggaataca
      421 tctgcgataa acctgagtgc ccccacgtca gatacgtcac taactcatgc ggcagccgtg
      481 cctgcccgtc ctgcggaaaa aaggccacag acctgtggat agcgacacag ctgaatcgtc
      541 ttcctgactg cgactgggta cacctggtct tcaccctgcc ggacacgcag tggccggtgt
      601 tcgaaagcaa ccggtggctg ctgaatgacg tgtgccgtct ggcggtggag aatctgctgt
      661 atgccgcccg gaaacggggg caggaacccg gtatcttctg cgccatccac acgtatggcc
      721 gtcgtctcaa ctggcatccg catgtacatg tgtctgtaac ctgtggaggt ctgaataagc
      781 atggtcagtg gaaaaagctg agcttcctga aagacgcgat gcgttcgcgg tggatgtgga
      841 atatgcggca acggcttctg aaagcgtggt cagaggggct ggcgatgccg gagtcgttgt
      901 cacatatcac gacggaatca cagaggagaa gcctggtgct gaaagccgga ggaaaatact
      961 ggcatgtgta catgtcgaaa aaaacggccg gcgggaggaa tacggcgcgc tacctgggtc
     1021 gttatctgaa gaagccgccg atagcggcct cccggctggc acattacaac ggagggggag
     1081 cctgagcttc cgttacctgg accacaaaac gggagaaacg gcgacggaaa cgctgacaca
     1141 gcgtgagctg gtcgcgaggc tgaaacagca catcccggag aagtttttta agatggtgag
     1201 gtacttcggg ttccttgcca accgggtgtg tggagagaag ctgccgcagg tgtaccgtgc
     1261 tctggggatg gataaaccgg aaccagtggc gaaagtgtgc tatgcacaaa tggtgaaaca
     1321 gttcctgagt cgtgacccgt tcgaatgcgt gctgtgtggc ggccggatgg tataccgccg
     1381 ggccatcgcg ggactgaatg tggaggggct gaagaaaaac gcgcgggata tcagtctgct
     1441 gaggtatatg ccggcctgac acaggctaag tgtgcccggg aatcggtgca agtataaaaa
     1501 acagcctgtt gcggtataag aatcgaccat gcagagagag aaaacaccgg agtggaggga
     1561 aaagcagaaa agctcacgtg gcataaggcg tggacagcgg tatcgt
//
LOCUS       DAJVRP010000240         1598 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVRP010000240 DAJVRP010000000
VERSION     DAJVRP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.247"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            154..477
                     /locus_tag="OQN12_004138"
     CDS             154..477
                     /locus_tag="OQN12_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCS1656259.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="OQN12_004139"
     CDS             474..890
                     /locus_tag="OQN12_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1656260.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..1380
                     /locus_tag="OQN12_004140"
     CDS             856..1380
                     /locus_tag="OQN12_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656261.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            1377..>1598
                     /locus_tag="OQN12_004141"
     CDS             1377..>1598
                     /locus_tag="OQN12_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656262.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLS"
BASE COUNT          389 a          379 c          456 g          374 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga ccgggcgtct ggcccgctcc attggttatt acgttcccaa aaaaacgacg
     1081 cgtcgccccg gactgatggt gaaaatttcc cctgaccaga aaaacgggca ggggaatcgc
     1141 cgttttcctg aaggtgctcc ttattatccg gcgtttctgt attacggcgt tcgccattca
     1201 gcgtatggaa tggataaaaa ggataagcgg cagaagaagc accattcttc gactttccgg
     1261 ctggccccgc gtaataactt tatggctgac gttattgagc gtcggcgtca ctggacgcag
     1321 gaactgttgt cccgtgagct gcagcgttcg ttacgtccgg taaaaaggaa gcataaatga
     1381 aactgacaac gataattgcg gcactgcgtg agcgatgccc gcgttttgag gagcgtgttg
     1441 gtggcgcggc gcagtttaag gcgatccctg atgccggaaa acttcgcctg cctgctgcct
     1501 atgtggttcc ctctgacgat gcgccggggg aacaaaaatc acagaccgat tactggcagg
     1561 atttgactga aggcttctcc gtgattgtgg tgctcagc
//
LOCUS       DAJVRP010000241         1597 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVRP010000241 DAJVRP010000000
VERSION     DAJVRP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.248"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..636)
                     /locus_tag="OQN12_004142"
     CDS             complement(<1..636)
                     /locus_tag="OQN12_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1656263.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="OQN12_004143"
     CDS             complement(750..1322)
                     /locus_tag="OQN12_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1656264.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          475 a          336 c          318 g          468 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJVRP010000242         1538 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVRP010000242 DAJVRP010000000
VERSION     DAJVRP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.249"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(910..1386)
                     /locus_tag="OQN12_004144"
     CDS             complement(910..1386)
                     /locus_tag="OQN12_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1656265.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          347 a          410 c          385 g          396 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tcctggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DAJVRP010000243         1533 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVRP010000243 DAJVRP010000000
VERSION     DAJVRP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.250"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..785)
                     /locus_tag="OQN12_004145"
     CDS             complement(<1..785)
                     /locus_tag="OQN12_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCS1656266.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVA"
     gene            complement(797..1286)
                     /locus_tag="OQN12_004146"
                     /pseudo
     CDS             complement(797..1286)
                     /locus_tag="OQN12_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1290..>1533)
                     /locus_tag="OQN12_004147"
     CDS             complement(1290..>1533)
                     /locus_tag="OQN12_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656267.1"
                     /translation="FKREVLASADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKG
                     RNTRATTGDAQRLNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          340 a          427 c          391 g          375 t
ORIGIN      
        1 tacgcaacca tgcgatcctt attccccaca ccacggcctt tcagccattt aaccgcgcgg
       61 atattcagcg gaacaccgtt ctggtgatag ctgatggtgt tggtctgaag gttcgtcaac
      121 agggactggt tacctgcaga tgaaacgatg atgctggaca acagagcaaa ctgctcaggc
      181 gggatcagca aatcacgcgg aaccacagag taaccagaag cggcccacgc atcagacagc
      241 acctggttaa tgcttgcgcg gatttcgtcc ggtgttgagg ttgcccacgt tttggcagcg
      301 ttgttgacag gcacgccgtc cagggtaaca aggcctttca ggtttaatgc ggaatcgcca
      361 acatatacct gttcatcgtt atccatctgc catttcagtt gcataccgtc atacttctgc
      421 gtatcaatcg ggcggccgac ctgctgagca gcctgcaatt ctatgaccgt ccagccaagt
      481 tccatccccc acaggttcag cgggttaccg gatttgccga tatccacgtt cacgccagca
      541 atagcggttg agtctttgcc tacccagttt tttccattcg gatttgcgcc agtacccgca
      601 gcggcgaagc tggtattcgt ccagctggaa atgtcatctg cgatggaggc gtcttcacgc
      661 agttgaatat cgcggctcca ggtgtacccc accagtggca ggttcagcgt ctggtcgagt
      721 ctctccagct ccccgatgag aaaggcacca gagctgtcaa cggttgcctg atcaaaagta
      781 atcattcgtc tgttccttaa atcttccagg aaatttctgc attgccgtca gcatcaccgg
      841 cacctgtgaa ttcagcgttg gtcagcacca cgtttttgcc actgactgac gtggacatga
      901 atccacccag aggcactttg atggattcat cagtggagat cgcctttttt gatagtgctg
      961 gcatcaaaat cagaaccgag attaacggtc acgtagccac gcttcatggc gtcgcccggg
     1021 aagttcttgc ctgttcccac ctggcgaacc atgtccggct gcgacgtggt cggataaggg
     1081 cgcacgtaga tccccttcac cttgtctgcg gtatcaccat ctgccagcgg cacgaaaaaa
     1141 ccgtcatcat cgtatttacc agccaggcca taggcagcga aggcgttatc ggatttaagg
     1201 accaccggtt cgacggttaa gtcctgcggg cgagagacag ccccggcaat gccaacaggc
     1261 atccggtaca gaaatacatt attcattttt taccctttac ggtttgccca gaattcagcg
     1321 ttttgtttgt tcagggaagc gatactggtc atgcccatgt ttaggcgctg tgcatcgccg
     1381 gtggtggcgc gggtgtttcg ccctttggca acctcagaca cggcattaaa cgccatgtcg
     1441 accgattgtt tcggtaattt gcggatatcc gcatcaccga ctatctggcg aaccagcgtt
     1501 ttgtcagcag aagccagaac ctcgcgtttg aac
//
LOCUS       DAJVRP010000244         1530 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVRP010000244 DAJVRP010000000
VERSION     DAJVRP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.251"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(334..480)
                     /locus_tag="OQN12_004148"
                     /pseudo
     CDS             complement(334..480)
                     /locus_tag="OQN12_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            534..716
                     /locus_tag="OQN12_004149"
     CDS             534..716
                     /locus_tag="OQN12_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCS1656268.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(817..1146)
                     /locus_tag="OQN12_004150"
     CDS             complement(817..1146)
                     /locus_tag="OQN12_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCS1656269.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1318..>1530)
                     /locus_tag="OQN12_004151"
     CDS             complement(1318..>1530)
                     /locus_tag="OQN12_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656270.1"
                     /translation="VHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIY
                     GYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          416 a          354 c          359 g          401 t
ORIGIN      
        1 tagcaatgtc tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt
       61 ccctgaaggc gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga
      121 acaaagctga ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg
      181 gtatccaacc cgcgaatatc agcatgattc accgtcggat ttactgctac gtcgccctca
      241 gggaattaaa cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt
      301 catatcagcg tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca
      361 tggcaggagt tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg
      421 cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag
      481 caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc
      541 acccggtcat tggagtagta accaaagcag atttagccag catggaacaa atatctttgg
      601 tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata
      661 acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc
      721 gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga
      781 gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat
      841 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
      901 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc
      961 ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag
     1021 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     1081 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     1141 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     1201 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc
     1261 atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt tcagaaatta
     1321 tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg ccgtttccat
     1381 gttcagccac acataaccat agattggtgt ttccacgact ttcaggtcat ggtggttatc
     1441 gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg
     1501 ctgcggatta ccttcgtaca gcgcatgaac
//
LOCUS       DAJVRP010000245         1523 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVRP010000245 DAJVRP010000000
VERSION     DAJVRP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.252"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..425
                     /locus_tag="OQN12_004152"
     CDS             <1..425
                     /locus_tag="OQN12_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1656271.1"
                     /translation="VYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQY
                     LNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDETE
                     FQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            425..715
                     /locus_tag="OQN12_004153"
     CDS             425..715
                     /locus_tag="OQN12_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCS1656272.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            712..1266
                     /locus_tag="OQN12_004154"
     CDS             712..1266
                     /locus_tag="OQN12_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS1656273.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            1407..>1523
                     /locus_tag="OQN12_004155"
     CDS             1407..>1523
                     /locus_tag="OQN12_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCS1656274.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQM"
BASE COUNT          384 a          341 c          435 g          363 t
ORIGIN      
        1 tcgtttactg gactccggtc ggtggggcga tcacgcctcg cgaacgaaaa ctggtatcag
       61 gcttcgttga ttacctgtgc gaatcagtag gtcgggaaca cactccagcc ctgagtgatg
      121 ccgcagagca ataccttaat acagttgcga catgcagaac ccgggatacg gcattgctaa
      181 agtcgtttga cgctttccgc gagtgggtaa ccattcaggc cggattttac accgagcata
      241 tttatcctga tggtagtcgt gggcgcaggg caaaatctat cgcatttgcg aacatggacg
      301 aaaccgagtt tcagcaggtt tataaatctg tactgaatgt gctgtggaac tggatcctgt
      361 tccgtaaatt ttcctctccg gaggaagtcg aaaatgtggc cgcgcagtta ctggagtttg
      421 cgtaatggtg gatttacgta aagcggcgcg ggggcagatg tgcaccgtca gaattcctgg
      481 ctactgcaat cacaatcccg aaacttctgt gctggcgcat tacaggctgg cggggacgtg
      541 cggaacagcg acaaaaccac acgatatgca ggcagcgatt gcctgtagct catgccacga
      601 tttaatcgac gggcgggtaa aaaccagcga ttacaccaaa gaagaattac gcctgatgca
      661 tgcagaaggt gtttttcgca cacaagaaat ctggagaaag gaaggttatt tatgatttac
      721 ccaacaaata caggcaaaag cggggaacac cttcgtctca ccacgctgga aagtgtctgg
      781 attcagggaa aactgcgcat gtgggggcgc tggtcgtata ttggcggcgg taagacggga
      841 aatatgttta accagttgct ggcatccaaa aaattgacga aaacagccgt caatgaagcc
      901 ctgcgcagga tgaaaaaagc gggaatagag aaacctgagc tggaagcgtt tttgcgagag
      961 atgatcaatg gcaagcaaaa gacctggctg gcgcattgta ctgatgcaga ggcgttatgt
     1021 attgatagag tcataagtga ggtgctggca gagcatcctg gattgattag tgtcctccgg
     1081 caacggtatg aagggcgggg gatgactaag cgtaaaatgg ctgaattgct gaatgacgca
     1141 caccctgagt ggtgtttccg gacgtgctgc agtcgggtag atgtatggct aaatcttgct
     1201 gaatatatgc tctatctacc gatgcgtgat gcattctctt ccggggatct aaaaaccgtt
     1261 tgttgactca atctgttatc cggggctata ttcctcacgc gccagcaaaa tctggcgtcg
     1321 ggattggcgt cccggatgtt tacggagcga tatgagacgc gcccgcgtct tttttcatat
     1381 cgtttgcaca gtcacattcg cgatttatgg cgggctgtgt gggggagccg aaaggctcgc
     1441 cggtttccgt acccggttac gccaaccctg cacagttcgc caccagttcg attggcgtcg
     1501 tcggtggcga taatttccaa atg
//
LOCUS       DAJVRP010000246         1494 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVRP010000246 DAJVRP010000000
VERSION     DAJVRP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.253"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(108..620)
                     /locus_tag="OQN12_004156"
     CDS             complement(108..620)
                     /locus_tag="OQN12_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCS1656275.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            815..>1494
                     /locus_tag="OQN12_004157"
     CDS             815..>1494
                     /locus_tag="OQN12_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656276.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDA"
BASE COUNT          449 a          325 c          374 g          346 t
ORIGIN      
        1 ccaatgcctt ggggggtagc ggtaatttta ctcattatcc ttcaaatcag attctgttgt
       61 cagaggaatg ggggaagctg gattcgcaag agctttttat gggcagttta gctaaaaaaa
      121 ggcaacacga agctaaaact accaacataa agtgctgacc agctagcagc tgaaatcaga
      181 ctcactgtat acactttcac tggatgaagt tgcaacattc ctgccaccaa aggagcaatg
      241 taacgcagca cggcgataaa gcgcgaagtg aaaagtattg acacagagtt attttgcaac
      301 tgaaggcgaa cccgttctat tgtattggaa cggctcgata ttatacatgc aagttgaggg
      361 atatgcccaa ttattatacc aagatggtaa ttaacgatag taccgcacca tgcgccactc
      421 atgaccgtga ttccagcttc ccatggtgaa agagtcgtct tgctgactgt aattacagtg
      481 gccatcataa ttgatgctgg tggcaatatc gccgaaatga gtaaagtgga ttttgtgaac
      541 gctattataa aaagaagccc ccataggcga tatggatgaa gtacaaaata gttcatcaaa
      601 gcatttatcc actccatcaa tgtctttcct tctgtgaatt tttctttgct atttaaccgt
      661 gatacgactt aatttaggct taaacacctt acatttacaa ggtactcctg tggggggccc
      721 tgccacggag cgtcgcagga gtgagtgatg aggaagaatt aaagcaacct attgtgacag
      781 gcagcgataa cagtaaatca caggaagcat aatcatggca aaaccggact gggaggccat
      841 cgaatcggca taccgggccg gagtccttag cctccgtgat ataggcgaga aatacggcgt
      901 tacagaaggg gctatcagga agagggccaa aaagcttgat tgggcacgca gtggcggtac
      961 gcaggtttgc aaaaatggta cgcaaaaaag gaaagtgcgt accagcagaa agcctgccat
     1021 tactggcctt acacaaaaaa gtacgcaact aaaaacagaa tctacaccgg atacgaaacc
     1081 gatacgcgga atgcgtaccg atcccccgac taacccattc caacccggta accagcaggc
     1141 attaaaacac ggtggttatg cccgtcgcct tctgctcaaa gatgaggtga tagaggacgc
     1201 taaagcgttg acgctcgagg acgaattatt tcgccttcgt gctaacaacc ttgtcgctgc
     1261 agagaatatt ggtcggtggt tggtgtcgct ggaagatgct aatggggacc aggaaaggaa
     1321 gatgctgatt gaaaatatca gcgccgccga gaaagcaatg atgcgcaata cagttcgtat
     1381 tgagtccatc gttggcacgc ttgcgacggt aggaaaaata tttgctgata cagcctaccg
     1441 caaggccgcc actgataagg tgtctctgga ggctgatcgt cttcgccgtg atgc
//
LOCUS       DAJVRP010000247         1368 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVRP010000247 DAJVRP010000000
VERSION     DAJVRP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.254"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>1368
                     /locus_tag="OQN12_004158"
     rRNA            <1..>1368
                     /locus_tag="OQN12_004158"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          341 a          318 c          432 g          277 t
ORIGIN      
        1 gctaataccg cataacgtcg caagaccaaa gagggggacc ttcgggcctc ttgccatcgg
       61 atgtgcccag atgggattag ctagtaggtg gggtaacggc tcacctaggc gacgatccct
      121 agctggtctg agaggatgac cagccacact ggaactgaga cacggtccag actcctacgg
      181 gaggcagcag tggggaatat tgcacaatgg gcgcaagcct gatgcagcca tgccgcgtgt
      241 atgaagaagg ccttcgggtt gtaaagtact ttcagcgggg aggaagggag taaagttaat
      301 acctttgctc attgacgtta cccgcagaag aagcaccggc taactccgtg ccagcagccg
      361 cggtaatacg gagggtgcaa gcgttaatcg gaattactgg gcgtaaagcg cacgcaggcg
      421 gtttgttaag tcagatgtga aatccccggg ctcaacctgg gaactgcatc tgatactggc
      481 aagcttgagt ctcgtagagg ggggtagaat tccaggtgta gcggtgaaat gcgtagagat
      541 ctggaggaat accggtggcg aaggcggccc cctggacgaa gactgacgct caggtgcgaa
      601 agcgtgggga gcaaacagga ttagataccc tggtagtcca cgctgtaaac gatgtcgact
      661 tggaggttgt gcccttgagg tgtggcttcc ggagctaacg cgttaagtcg accgcctggg
      721 gagtacggcc gcaaggttaa aactcaaatg aattgacggg ggcccgcaca agcggtggag
      781 catgtggttt aattcgatgc aacgcgaaga accttacctg gtcttgacat ccacggaagt
      841 tttcagagat gagaatgtgc cttcgggaac cgtgagacag gtgctgcatg gctgtcgtca
      901 gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta tcctttgttg
      961 ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga taaactggag gaaggtgggg
     1021 atgacgtcaa gtcatcatgg cccttacgac cagggctaca cacgtgctac aatggcgcat
     1081 acaaagagaa gcgacctcgc gagagcaagc ggacctcata aagtgcgtcg tagtccggat
     1141 tggagtctgc aactcgactc catgaagtcg gaatcgctag taatcgtgga tcagaatgcc
     1201 acggtgaata cgttcccggg ccttgtacac accgcccgtc acaccatggg agtgggttgc
     1261 aaaagaagta ggtagcttaa ccttcgggag ggcgcttacc actttgtgat tcatgactgg
     1321 ggtgaagtcg taacaaggta accgtagggg aacctgcggt tggatcac
//
LOCUS       DAJVRP010000248         1326 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVRP010000248 DAJVRP010000000
VERSION     DAJVRP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.255"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            116..913
                     /locus_tag="OQN12_004159"
                     /pseudo
     CDS             116..913
                     /locus_tag="OQN12_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          401 a          239 c          307 g          379 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga tggtttatct ggtgggttaa ttgatggtct
      901 gaagatgctt gctgtggctg ttactgctca aaaggctgca aatgtagcag gaggcttgtt
      961 aaaccttgca aacgctgtta cttcattcgg tgacgctgtt aataaagcta aagctggcaa
     1021 ggctggttct tcaggtggtg cgctaaataa agtcggtaga gttgtccttt atgctgagtt
     1081 ggtaaatttt gctcttgata atttaaaccc tgaagcagct aagaatccga acttacatca
     1141 atcaggtatt ggcacagaaa caaacttcaa ccctgaatac aaaggtgatt catctaagcc
     1201 aatagttcta tcgctgactg gtttaatggt gcaactaata aaattgctaa cttccaagaa
     1261 ccgatgaata acttcgtaaa taacacacaa tcaactccat cagctatcac aggtgacatg
     1321 gttaat
//
LOCUS       DAJVRP010000249         1272 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVRP010000249 DAJVRP010000000
VERSION     DAJVRP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.256"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            483..>1272
                     /gene="uacT"
                     /locus_tag="OQN12_004160"
     CDS             483..>1272
                     /gene="uacT"
                     /locus_tag="OQN12_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1656277.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGLMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAV"
BASE COUNT          294 a          292 c          279 g          407 t
ORIGIN      
        1 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
       61 atttaatgag ttatgtcttc tcaaatttcg cgatgcaata aacgggcata tattaggtag
      121 catgaccgtt ttaattataa ttttgcgact gagttcaaat tattgcccct caatctatgc
      181 cgctttcatt atttcaacat tgccgatcaa aagagcgcta tccagataaa aacgattaaa
      241 tgcgagagtg cgacatgcca gaatgattaa tgaaatatca atatgagaaa attacagata
      301 attccattgt caggttctct tgtcattttc gtggtttaag cacgattatg tgattgcctc
      361 cgcaattcct gtctctaact ccccttcctc gcaaaaactg gcactccacg agcatgtgtt
      421 tagacagttt cattaacgta aacggttgct ttttattctg gcgggcgaaa ggagaaacac
      481 tgatgagcgc catagattcc caacttccct catcttctgg gcaagaccgc ccaactgatg
      541 aggttgaccg catattatca ccaggaaagc tgatcatact cggtctgcaa cacgtccttg
      601 tcatgtacgc aggtgcagtc gctgttcctc ttatgattgg tgaccgactg ggcctctcaa
      661 aagatgctat tgcgatgctc attagctcgg atctcttttg ctgcgggatc gtcacattat
      721 tgcaatgtat cggtatcggc cgctttatgg ggatccgcct gccggtgatt atgtcggtga
      781 cctttgctgc tgtaacacca atgatagcca ttgggatgaa cccggatatc ggcctgctgg
      841 ggatatttgg tgccactatc gccgcgggtt ttatcaccac attattagcg ccacttattg
      901 gtcgcttgat gcctttattc ccgccactgg ttaccggtgt ggttattact tctattgggc
      961 ttagcatcat tcaggtgggt attgactggg ccgctggagg taaagggaat ccgcaatatg
     1021 gtaatcccgt ttatttaggt atctcctttg ccgtcttaat ttttatcttg ctcattactc
     1081 gctatgcgaa aggattaatg tccaacgtcg ccgtattact ggggattgta tttggctttt
     1141 tactttcgtg gatgatgaat gaagtcaatt tatccgggct acatgatgcc tcgtggtttg
     1201 cgattgtcac cccgatgtcg tttggtatgc cgattttcga tcccgtttcc attctgacca
     1261 tgactgccgt gt
//
LOCUS       DAJVRP010000250         1259 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVRP010000250 DAJVRP010000000
VERSION     DAJVRP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.257"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            34..435
                     /locus_tag="OQN12_004161"
     CDS             34..435
                     /locus_tag="OQN12_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1656278.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OQN12_004162"
     CDS             447..818
                     /locus_tag="OQN12_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1656279.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1259
                     /locus_tag="OQN12_004163"
     CDS             805..>1259
                     /locus_tag="OQN12_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1656280.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPV"
BASE COUNT          299 a          281 c          390 g          289 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatcttgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccggga tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtgga
//
LOCUS       DAJVRP010000251         1155 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVRP010000251 DAJVRP010000000
VERSION     DAJVRP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.260"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            28..876
                     /locus_tag="OQN12_004164"
     CDS             28..876
                     /locus_tag="OQN12_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1656281.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
BASE COUNT          328 a          283 c          266 g          278 t
ORIGIN      
        1 cacttcggtg atgaaaggaa agagaaaatg ttgaatgtag ctattgaaaa ccagaacggg
       61 tggaattata gtgcacctgc acctcataaa acgggtgccg ggattgctac cccgacgatg
      121 actactgcgc ataaccgcgc tcaggcggtt tttttatgcg tcaagcacag ccacattcag
      181 attatggtgg ggcgtgcagg gcagccgcaa ggctggccgg tttcggtagt caccggttgt
      241 agcaaccctg tgcgtctcac cacccatgag attgctacct ctggtggtga gtcatttaaa
      301 ctgactatcg aggctgccat catggctact atccttaccc tttctcaccc tgacgcaacc
      361 atcgaaaatg gtcgcgctgt cactacgtct gttgcggttg cggagttctt ccggaaaatg
      421 cacaaaaacg ttattcagaa gatcgaaact ctcgaatgct cgccggagtt taaccggctt
      481 aattttaagc ccgtcaccta taccgacgcc aaaggcgaaa aacgcccaat gtaccaaatc
      541 accaaaaacg gcttcgtttt cctggtgatg ggcttcactg gcaaaaaagc cgccgcattc
      601 aaggaagcct acattgctga gttcgaccgc atggaggccg aactgcgcca gaataacacc
      661 ccacctgctg acaagatgat tccgggtgat ggtcgcactc tggttgttca cttcgacaaa
      721 ttcggcaatg tcgaattcac cgaaaccgtt cctgatggcg cactggtctg taccctggag
      781 actttccggt tttatctgga aaaacaggga tggactcttg ttaaccgggg cgcaattaaa
      841 aatatgactg tggaacaatt actttcttta aaataaaaat ggagctacgg atatgaaccc
      901 tatgcttgat tattgtttta cctctgcacc tcagcagacc ataacacaca ccggaatcaa
      961 aaatttgtta tggataatca aaaaaaacct ccagcagaaa ggtgatcccg atttaattcc
     1021 ctgtgcttat ggcctgtgtg acagacaagt aagccctgat aaggcttaca ctgctgaact
     1081 tatttacatg cacggaacca tgatgaggaa aaagaaacgc aaatattgta gtgaaatctg
     1141 cgccgaaaaa gacca
//
LOCUS       DAJVRP010000252         1155 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVRP010000252 DAJVRP010000000
VERSION     DAJVRP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.261"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            2..385
                     /locus_tag="OQN12_004165"
     CDS             2..385
                     /locus_tag="OQN12_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1656282.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..1030)
                     /locus_tag="OQN12_004166"
     CDS             complement(386..1030)
                     /locus_tag="OQN12_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1656283.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          296 a          262 c          277 g          320 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcgcgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcattgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaa
//
LOCUS       DAJVRP010000253         1111 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVRP010000253 DAJVRP010000000
VERSION     DAJVRP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.262"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..1019
                     /locus_tag="OQN12_004167"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OQN12_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          272 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag c
//
LOCUS       DAJVRP010000254         1083 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVRP010000254 DAJVRP010000000
VERSION     DAJVRP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.263"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            36..>1083
                     /gene="cadB"
                     /locus_tag="OQN12_004168"
     CDS             36..>1083
                     /gene="cadB"
                     /locus_tag="OQN12_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCS1656284.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASAILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASD"
BASE COUNT          227 a          243 c          283 g          330 t
ORIGIN      
        1 gactccaaat tcaaaaatga aattaggaga agagcatgag ttctgccaag aagatcgggc
       61 tatttgcctg taccggtgtt gttgccggta atatgatggg gagcggtatt gcattattac
      121 ctgcgaacct agcaagtatc ggtggtattg ctatctgggg ttggattatc tctattattg
      181 gtgcaatgtc gctggcgtat gtatatgccc gactggcaac aaaaaacccg caacaaggtg
      241 gcccaattgc ttatgccgga gaaatttccc ctgcatttgg ttttcagaca ggtgttcttt
      301 attaccatgc taactggatt ggtaacctgg cgattggtat taccgctgta tcttatcttt
      361 ccaccttctt cccagtatta aatgatcctg ttccggcggg tatcgcctgt attgctatcg
      421 tctgggtatt tacctttgta aatatgctcg gcggtacctg ggtaagccgt ttaaccacta
      481 ttggtctggt gctggttctt attcctgtgg tgatgactgc tattgttggc tggcattggt
      541 ttgatgcggc aacttatgca gctaactgga atactgcgga taccactgat ggtcatgcga
      601 tcattaaaag tattctgctc tgcctgtggg ccttcgtggg tgttgaatcc gcagcagtaa
      661 gtactggtat ggttaaaaac ccgaaacgta ccgttccgct ggcaaccatg ctgggtactg
      721 gtttagcagg tattgtttat atcgctgcga ctcaggtgct ttccggtatg tatccgtctt
      781 ctgtaatggc ggcttccggt gctccgtttg caatcagtgc ttcagctatc ctcggtaact
      841 gggctgcgcc gctggtttct gcattcaccg cctttgcgtg cctgacttct ctgggctcct
      901 ggatgatgtt ggtaggccag gcaggtgtac gtgccgctaa cgacggtaac ttcccgaaag
      961 tttatggtga agtcgacagc aacggtattc cgaaaaaagg tctgctgctg gctgcagtga
     1021 aaatgactgc cctgatgatc ctcatcactc tgatgaactc tgccggtggt aaagcatctg
     1081 acc
//
LOCUS       DAJVRP010000255         1040 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVRP010000255 DAJVRP010000000
VERSION     DAJVRP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.264"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..769
                     /locus_tag="OQN12_004169"
     CDS             <1..769
                     /locus_tag="OQN12_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1656285.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKPG"
     gene            790..>1040
                     /locus_tag="OQN12_004170"
     CDS             790..>1040
                     /locus_tag="OQN12_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCS1656286.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
BASE COUNT          337 a          231 c          234 g          238 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagacac agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc
      601 aggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt
      661 caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag
      721 aagactatct gaaagtgtcc catgcatgcc ccataagaaa ccggggtaaa ataaaaactt
      781 gcaaaatcaa ttggtttacc tttaatccct gtcacgttac gcgcgtggca gaggcgttac
      841 ggattgctga aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt
      901 gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa
      961 atactgctca gtaatgaaaa tgttgatgtg cagaacgtct ggcgcgatca gcaagaaaca
     1021 ttactgactt acctgcaaag
//
LOCUS       DAJVRP010000256         1029 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVRP010000256 DAJVRP010000000
VERSION     DAJVRP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.265"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            207..>1029
                     /locus_tag="OQN12_004171"
     CDS             207..>1029
                     /locus_tag="OQN12_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1656287.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
BASE COUNT          345 a          248 c          149 g          287 t
ORIGIN      
        1 tttttttaca ttaaaccgat ccagtttagg atagtgtaaa gcagaagttc ttcaatatat
       61 aacatatgga gttaatatgc ttacacaaac tatatatccg cctcctccag aagttcctgt
      121 ctactgctga agggaaccaa taacattgca aagcagataa aaaagaatcc aacacctctc
      181 aacataaaga atacgccctg atatttatgc tcccgacaaa taacaatcac agattaattt
      241 caaattcgtt ctccacttat tcaatcgaca ctagccgcgc atatgaaaat tacctaaccc
      301 attggactga atggaaaaat aaccgcatac aagaagaaca acgagacatc gcttttcagc
      361 gactagtatc atgtctacaa aaccaagaga cgaacctgga cttgtctgaa ttaggcctga
      421 caacattacc tgaaatcccc ccgggaatta aatcaattaa tataagtaaa aataatttaa
      481 gcttaatctc cccattacct gcgtccctta cacaacttaa tgtcagctat aacagactta
      541 ttgaactgcc tgctttgcct caaggactta aattattgaa tgcgtcccac aatcaactaa
      601 tcacactacc cacactcccc atatctttga aggagcttca tgtctcaaat aatcaattat
      661 gttctcttcc tgttttacca gaactactgg aaacattaga tgtatcatgt aatgggctgg
      721 cagttttacc acctttacca ttttctttac aagagattag cgcaataggg aatcttctta
      781 gtgaactccc ccctctacct cacaacattc actccatatg ggcaatcgac aatatgttaa
      841 ccgatattcc atacctgccg gaaaatttaa ggaacggtta ttttgacata aatcagataa
      901 gtcatatccc ggaaagcatt cttaatctga ggaatgaatg ttcaatagat attagtgata
      961 acccattgtc atcccatgct ctgcaatccc tgcaaagatt aacatcttcg ccggactacc
     1021 acggcccgc
//
LOCUS       DAJVRP010000257         1021 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVRP010000257 DAJVRP010000000
VERSION     DAJVRP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.266"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>1021
                     /locus_tag="OQN12_004172"
     CDS             <1..>1021
                     /locus_tag="OQN12_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1656288.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGL"
BASE COUNT          316 a          192 c          265 g          248 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag cctgagttat agtacggtat ggagaggcat
      241 aacattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac atttggtcaa aatgtggatg ggacattagc
      661 ccttatagag gcacccggag caacgggggt taacgttaat ggctggccgg gactgagtac
      721 tgattttcgt gggtatgcaa tactgccagt gcagccatac cgaagagacg atgttattct
      781 ggatgaaaaa acgataggta agaattacga cttaccacaa actagtcaat tggttgtacc
      841 gacagctgga gccgttgtgc ctgcgacatt ggcagtgaag agcggggata aaggattagt
      901 aaccttaaaa cagaaagagg gtaaacccgt ccccttcggc gctgtgatta gttatagcaa
      961 agacactgaa aatatggccg gcattgtagg tgaagatggt attgcttatg tttctggctt
     1021 g
//
LOCUS       DAJVRP010000258         1010 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVRP010000258 DAJVRP010000000
VERSION     DAJVRP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.267"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(50..190)
                     /locus_tag="OQN12_004173"
     CDS             complement(50..190)
                     /locus_tag="OQN12_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656289.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="OQN12_004174"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="OQN12_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS1656290.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          292 a          205 c          235 g          278 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt
//
LOCUS       DAJVRP010000259         1008 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVRP010000259 DAJVRP010000000
VERSION     DAJVRP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.268"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..228
                     /locus_tag="OQN12_004175"
                     /pseudo
     CDS             1..228
                     /locus_tag="OQN12_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            374..>1008
                     /locus_tag="OQN12_004176"
     CDS             374..>1008
                     /locus_tag="OQN12_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1656291.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          251 a          242 c          305 g          210 t
ORIGIN      
        1 tgatatcgca aggcacaccg agcagggaat tctggttatc ggtggggctg ttcgccagaa
       61 aaaatttgac ccgttgtcag ttgatctgcc cgactggttg ccagaaacac tctggcatga
      121 gtgggtccag ttcaggcagg cattgcgaaa accgattcga acggagctgg gcgctaacgg
      181 ggcgatacgg gaactggaaa aattccgtca gcagggtttt acacctgagc aggtgattcg
      241 acacagcatc gccaatgaat accagggtct gttcgcgccg aaaggtgttc ggcctgagac
      301 gttgctccga caggttaaca ccgtctcgtt gccggacagt gcgatcccgc taggcttcag
      361 ggggtaacag accatgaaaa atattgcgac aggaggcgtt ctggagcgta tccgcagact
      421 gaccccacca catgtaaccg ccccattcag aacggttgcg gagtggcgcg agtggcaact
      481 tgctgaaggc cagaaacgtt gcgaggagat caaccgcctg aatcgtcagt tgcgggtgga
      541 aaaaatcctc cgctccggca tccagccgtt gcaccggaag tgttcgtttg cgaattaccg
      601 ggcgcagaac gacggtcagc gacatgcact gagtcaggcg aaatccatag ctgacgaact
      661 gatgacaggc tgtacgaatt ttgtgttcag cggtaagccc ggcaccggaa aaaatcatct
      721 tgcagccgcg attggcaacc ggctgatggc gaaggggcgt agcgtgatta tcgtcaccgt
      781 gtccgatgtc atgagcgtgt tgcatgagag ctacgacaac ggcaaatccg gtgaaaaatt
      841 tttacaggag ctttgtggtg ttgacctgct ggtcctggat gaaattggca tgcagcggga
      901 tacgaaaaac gagcaggtgg tactgaacca gattgttgat cgccggacgg catcgttacg
      961 cggtgtgggg atgctgacaa atattaacca tgcagcgatg aatacact
//
LOCUS       DAJVRP010000260         1000 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVRP010000260 DAJVRP010000000
VERSION     DAJVRP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.269"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..1000
                     /locus_tag="OQN12_004177"
                     /pseudo
     CDS             1..1000
                     /locus_tag="OQN12_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          266 a          248 c          289 g          197 t
ORIGIN      
        1 gttgttgacc agaaatacac ggtggcagat gccgccaaag ctatggatgt tggcctttcc
       61 acaatgacaa gatgggtcaa acaactgcgt gatgagcgtc agggcaaaac accaaaagcc
      121 tcccccatta ccccggaaca aattgaaatc cgtgagctca ggaaaaagct acaacgcatt
      181 gaaatggaga atgaaatatt aaaaaaggct accgcgctct tgatgtcaga ctccctgaac
      241 agttctcgat aatcgggaaa ctcagagcgc attatcctgt ggtcacactc tgccatgtgt
      301 tcggggttca tcgcagcagc tacagatact ggaaaaaccg tcctgaaaaa ccagacggca
      361 gacgggctgt attacgcagt caggtacttg agctacatgg catcagccac ggttcggccg
      421 gagcaagaag catcgccaca atggcaaccc ggagaggcta ccagatggga cgctggcttg
      481 ctggcaggct catgaaagag ctggggctgg tcagctgtca gcagccgact caccggtata
      541 aacgtggtgg tcatgaacat gttgctatcc ctaactacct tgaaaggcag ttcgccgtga
      601 ccgagccaaa tcaggtgtgg tgcggtgatg tgacctatat ctggacgggt aagcgctggg
      661 cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt
      721 tctcgccgga cagcaggctc accatgaaag cgctggaaat ggcatgggaa acccgtggta
      781 agcccggcgg ggtgatgttc cacagcgatc agggcagtca ttatacgagc aggcagttcc
      841 ggcagttatt gtggcgatac cagatcagac agagtatgag ccggcgcgga aactgctggg
      901 ataacagccc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg ccggtggtgg
      961 gttacgtaag cttcagcgag gcagctcacg ccataacgga
//
LOCUS       DAJVRP010000261          986 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVRP010000261 DAJVRP010000000
VERSION     DAJVRP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.270"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            284..457
                     /locus_tag="OQN12_004178"
     CDS             284..457
                     /locus_tag="OQN12_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656292.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(529..678)
                     /gene="hokB"
                     /locus_tag="OQN12_004179"
     CDS             complement(529..678)
                     /gene="hokB"
                     /locus_tag="OQN12_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS1656293.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(779..919)
                     /locus_tag="OQN12_004180"
                     /pseudo
     CDS             complement(779..919)
                     /locus_tag="OQN12_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          276 a          222 c          213 g          275 t
ORIGIN      
        1 aacacacttc tacgcatgat gccgcgtaaa cgttcctgaa ggatatttaa agaaaacgcc
       61 tgtactaaaa ccgacccgtg gtacaggcga agaatacggg tctacatcgg aagcgcctat
      121 tatatttatt tgtatgacaa ataaaaccat aatccttgcc catacgtcca tctggcttat
      181 ttttaatcaa ttcacccgat ctttgatctc atcaacggta tcaaaataaa ccagcgtata
      241 acgttcaaat cgcacataag attcgataaa ccatggaggt tatatgaaaa aactggcact
      301 tattttgttt atgggaacgc ttgtttcctt ttatgccgat gccgggcgca aaccctgttc
      361 tggttcgaaa ggggggatct cacactgtac ggcaggcggc aaatttgtct gtaatgatgg
      421 ttctattagt gcatcgaaaa aaacatgcac taactgaagt gtaaaagggg tgccatgaga
      481 aaatgaatct gctagtcaga tgcgcgggga aaatccccgc gcttgccctt acctggacgt
      541 gcaggccatg agcgcagcaa cctccttatc accgtcccgg aaccgcagtt cgtagagcgt
      601 ttgtcgggtc aggagtgtga atgccagaat cgtaatgcag ataatgagca gacacaccac
      661 cagagggttg cgcttcatag cctttctcct tgccggatgg cgggtaagag gctaacaatc
      721 tgaattgctt ggttcattcg ttggcctcgg tttgtactga ccccaaaaag ttggacagtt
      781 aaacacgagg catataggtc tgattccgat attcatttgg agtcagacct tttaatttca
      841 ggctaattct tctgctgttg tagtattcaa tatattccgt aacagcatcc ttcagttcgc
      901 ttatattact gaactcatta gactggcccc ctgaatctcc agacaaccag tatcacttat
      961 ttaagtgata gtcttaatac tagttt
//
LOCUS       DAJVRP010000262          983 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVRP010000262 DAJVRP010000000
VERSION     DAJVRP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.271"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OQN12_004181"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OQN12_004181"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCS1656294.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAJVRP010000263          958 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVRP010000263 DAJVRP010000000
VERSION     DAJVRP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.272"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>958
                     /locus_tag="OQN12_004182"
     CDS             <1..>958
                     /locus_tag="OQN12_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1656295.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRAFSAFLDR
                     LSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTVAADAT"
BASE COUNT          285 a          235 c          157 g          281 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat tctccgcgtt ccttgaccgc ctttccgata
      841 ccgtctctgc acgcaatacc tccggattcc gtgaacaggt cgctgcatgg ctggaaaaac
      901 tcagtgcctc tgcggagctt cgacagcagt ctttcactgt tgctgctgat gccactga
//
LOCUS       DAJVRP010000264          931 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJVRP010000264 DAJVRP010000000
VERSION     DAJVRP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.273"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..269
                     /locus_tag="OQN12_004183"
     CDS             <1..269
                     /locus_tag="OQN12_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1656296.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..616
                     /gene="tnpB"
                     /locus_tag="OQN12_004184"
     CDS             266..616
                     /gene="tnpB"
                     /locus_tag="OQN12_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1656297.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            613..>931
                     /locus_tag="OQN12_004185"
     CDS             613..>931
                     /locus_tag="OQN12_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1656298.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          253 a          238 c          258 g          182 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcacca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gcaacatccc aaacggacag aacggcctgg catccggata taacccgtga
      601 taaaacaagg gaatgaacaa tgaactcccc gatgatattg agctgcttaa agccatgttg
      661 cgtaagcaac agagtcggct tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa
      721 attgaacggc tgaaagcgca actcgacagg ttgcgtcgta tgttgttcgg ccagagttca
      781 gagaaaaagc gtcataagct tgaaaatcag atccgacagg cagaaaaacg actgtcggaa
      841 ctggaaaacc ggctgaacac agccagaaat cttctggaag atgcatcgtc agtcacagat
      901 tcacctgaca ccagtccccc gtcagaaaac c
//
LOCUS       DAJVRP010000265          921 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVRP010000265 DAJVRP010000000
VERSION     DAJVRP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.274"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            31..510
                     /locus_tag="OQN12_004186"
     CDS             31..510
                     /locus_tag="OQN12_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656299.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
     gene            498..731
                     /locus_tag="OQN12_004187"
     CDS             498..731
                     /locus_tag="OQN12_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCS1656300.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            731..>921
                     /locus_tag="OQN12_004188"
     CDS             731..>921
                     /locus_tag="OQN12_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656301.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEA"
BASE COUNT          252 a          215 c          245 g          209 t
ORIGIN      
        1 atcatcaaac tgtcttggat agaagccgca gtggatgcgc acaaaacgtt aaatttcgaa
       61 cccagtggaa gaaagcgtat tggctttgac gtggctgaca gtggtacaga taagtgcgct
      121 aacgtttacc gtcacggatc cgttgttttc tgggccgacg aatggaaggc caaagaagat
      181 gaattactga agagctgcca gcgtacttat caggcggcgc tggagcgtga agcagatatt
      241 gtttacgact ctatcggtgt tggtgcgtct gccggtgcta aattctctga aattaacgct
      301 gaccggaaga gcgagaacgc atacgcgcga cgtgtgaatt accagaggtt taacgccggt
      361 gctggtgtgc atgagccaga tgacgagtac aacggcatcc ccaacaaaga ctttttcgca
      421 aatcttaagg ctcaggcatg gtggctgacc gtttcagaaa tacgtttaac gccattaaca
      481 acggagaaca gtatcctgtg gatgagctga tcagcataga ttctcgttgt ccgttgcttg
      541 aaaagctgaa actggaactg acaacacctc atcgtgattt cgaccgtaac ggacgtgtga
      601 tggtcgaaag taaaaaagac ctcgcaaaac gcgagatacc gtcaccaaac gttgctgatg
      661 cattcattat ggccttcgcg ccaatcgata catcgctgga tatctgggaa cagctgggga
      721 gacaggcctg atggcacgaa acaaacaagc cctgcggcga actgtgcagg ccacagctga
      781 tggttatgag aattttattg cccgagtagg gatgcagaca cctaaccagc actcagcatc
      841 cacctaccgg gctaatttca ccagtcgtaa ccgcatgctg gtggaatggt cctatcgttc
      901 atcctggatc atcggcgaag c
//
LOCUS       DAJVRP010000266          871 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVRP010000266 DAJVRP010000000
VERSION     DAJVRP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.275"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..550
                     /locus_tag="OQN12_004189"
     CDS             <1..550
                     /locus_tag="OQN12_004189"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656302.1"
                     /translation="DAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQA
                     IQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLT
                     GSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEV
                     ETALFNARALALKIYSMSGWNL"
BASE COUNT          249 a          161 c          213 g          248 t
ORIGIN      
        1 cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa ctcagcatga
       61 caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg gtgtgacaga
      121 taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct ccggcgggga
      181 gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca ctttgcttat
      241 tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag caaaaagcgg
      301 acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc gttatctgag
      361 aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca ttacagcaga
      421 agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg tgaatgaagt
      481 tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca tgtcaggctg
      541 gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt gtcagctcct
      601 gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg attcagcctc
      661 aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta gacagtgaga
      721 ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat ttgcatatgg
      781 aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat gtaaacggag
      841 gattttcttt ctcaaactca tggatagcag c
//
LOCUS       DAJVRP010000267          867 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVRP010000267 DAJVRP010000000
VERSION     DAJVRP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.276"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(196..>867)
                     /locus_tag="OQN12_004190"
     CDS             complement(196..>867)
                     /locus_tag="OQN12_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1656303.1"
                     /translation="QFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRRKKIRYR
                     DEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYKAWLLWC
                     YSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAELFGGEG
                     YEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKAAFSKQS
                     IAKVD"
BASE COUNT          217 a          237 c          168 g          245 t
ORIGIN      
        1 accactgagc taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact
       61 catctgacca ccagcacgtt taacgtcctg tgccattttt caggcataaa aaaaccgctc
      121 ggcggcgggt tttgcttact ttaccatcgc gtacaaaatc ggcaaagtat cagatttgca
      181 tgagatatat gccttttaat ctacttttgc aatactttgc tttgaaaatg ccgctttttg
      241 ttctgaacgt gttctcatca caaacaataa agcctcacta tccagtcggt gaaaaatgtg
      301 tttcattaca acccagtgac cagtaaatgt tttggaccag tttttggttg ttactcccgc
      361 cagtaatgcc agctcctgat attcataacc ttccccacca aaaagttctg cttttaccgc
      421 ctgcgccgcc agccagatta attttttcag gcgttcctgt gttttctctg caattttcct
      481 agtgccagaa tgagccttaa actcttccca cgcccagtgg gttatggtga tctgatactc
      541 ccagcaaata cttccgctgt aacaccacaa caaccaggct ttatagtgct cttcaagaga
      601 cagaacagcc cgccgccacg atgatgtcga aaacgcaacc tgactgacca gaggaattga
      661 cgcccccttc gccagtgatt gttttcccgg gattggtgga ttatcccgcg ttatcatttt
      721 tccggtcacc tcatcgcggt accggatttt tttacgcctg taacgccctg tatcgaacat
      781 ggcattctcc tgccaggctt caagctgacc ttttgtcgcc ccactcaaat cagcggtggc
      841 gataatgagc tgctcacgaa caaactg
//
LOCUS       DAJVRP010000268          863 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVRP010000268 DAJVRP010000000
VERSION     DAJVRP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.277"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..256)
                     /locus_tag="OQN12_004191"
     CDS             complement(<1..256)
                     /locus_tag="OQN12_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656304.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSM"
     gene            281..520
                     /gene="relB"
                     /locus_tag="OQN12_004192"
     CDS             281..520
                     /gene="relB"
                     /locus_tag="OQN12_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCS1656305.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            520..807
                     /gene="relE"
                     /locus_tag="OQN12_004193"
     CDS             520..807
                     /gene="relE"
                     /locus_tag="OQN12_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCS1656306.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          240 a          175 c          219 g          229 t
ORIGIN      
        1 acatgctaaa gcaaaaaata gatgaataag ttgagttgtg catatgtagc ctgaccgtca
       61 caaagtatat ggtgtctgta ccagtaagat gatggccgga ctctttaaaa aagagctgac
      121 ctgcacaata caggatggac ttagcaatgg ctgctcctgg cacaaagcgg acagtgatca
      181 ccgttcttac gactactttc tgacttcctt cgtgacttgc cctaagcatg ttgtagtgcg
      241 atacttgtaa tgacatttgt aattacaaga ggtgtaagac atgggtagca ttaacctgcg
      301 tattgacgat gaacttaaag cgcgttctta cgccgcgctt gaaaaaatgg gtgtaactcc
      361 ttctgaagcg cttcgtctca tgctcgagta tatcgctgac aatgaacgct tgccgttcaa
      421 acagacactc ctgagtgatg aagatgctga acttgtggag atagtgaaag aacggcttcg
      481 taatcctaag ccagtacgtg tgacgctgga tgaactctga tggcgtattt tctggatttt
      541 gacgagcggg cactaaagga atggcgaaag ctgggctcga cggtacgtga acagttgaaa
      601 aagaagctgg ttgaagtact tgagtcaccc cggattgaag caaacaagct ccgtggtatg
      661 cctgattgtt acaagattaa gctccggtct tcaggctatc gccttgtata ccaggttata
      721 gacgagaaag ttgtcgtttt cgtgatttct gttgggaaaa gagaacgctc ggaagtatat
      781 agcgaggcgg tcaaacgcat tctctgaacc aaagcatgac atctctgttt cgcaccgaag
      841 gtgacacttc tgctttgcgt tga
//
LOCUS       DAJVRP010000269          831 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVRP010000269 DAJVRP010000000
VERSION     DAJVRP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.278"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..326
                     /locus_tag="OQN12_004194"
     CDS             <1..326
                     /locus_tag="OQN12_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCS1656307.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="OQN12_004195"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="OQN12_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCS1656308.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          154 g          241 t
ORIGIN      
        1 ttagtgaaac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAJVRP010000270          802 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVRP010000270 DAJVRP010000000
VERSION     DAJVRP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.279"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            206..421
                     /locus_tag="OQN12_004196"
     CDS             206..421
                     /locus_tag="OQN12_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656309.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            524..802
                     /locus_tag="OQN12_004197"
                     /pseudo
     CDS             524..802
                     /locus_tag="OQN12_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          215 a          189 c          193 g          205 t
ORIGIN      
        1 gtgagaaatc cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc
       61 cgcttcggcg ggtttttgtt tttattttca acgcatttga agttctggat ggtgccggaa
      121 tagaatcaaa aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga
      181 gtgctactta tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg
      241 tgaagatggc ctttatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat
      301 tgtgtgtttg caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga
      361 cggatttatc ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatctg
      421 aataaaagct ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg
      481 gctgaactcc cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa
      541 atattacaat catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc
      601 atttaagtca gactggtacc agcatccctc atgcactgaa gaacaggccg aatggcttat
      661 tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca
      721 ctagataatc tccgtcaggc tcccttactc cgaacagcca ccgcgtccgt cccgcacatt
      781 ccagcaacgg atctggaggt aa
//
LOCUS       DAJVRP010000271          802 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVRP010000271 DAJVRP010000000
VERSION     DAJVRP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.280"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            69..341
                     /locus_tag="OQN12_004198"
     CDS             69..341
                     /locus_tag="OQN12_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656310.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            341..646
                     /locus_tag="OQN12_004199"
     CDS             341..646
                     /locus_tag="OQN12_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656311.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            646..>802
                     /locus_tag="OQN12_004200"
     CDS             646..>802
                     /locus_tag="OQN12_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1656312.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQAC"
BASE COUNT          260 a          178 c          192 g          172 t
ORIGIN      
        1 tcacagataa aacactctcc aggaaacccg gggcggttca gaaagtaaat gaaacagatg
       61 ctgaatacct ggtgcgtaaa ttcgatgagc ttgaagctaa atgtgcagca caggaaaaca
      121 aagtaatacc agtgtcaact gaactgccac cagcaaatga aagtgttttg ttattcgatg
      181 ctaacggaga aggctggcta attggctggc gttctctctg gtacacctgg ggacaaaaag
      241 aaaccggaga atggcagtgg acatttcagg tcggggacct tgaaaacgtc aatatcactc
      301 actgggcagt aatgccaaaa gcaccggagg ctggagcata atgaccactt ttaccgacaa
      361 agaactgatt aaagaaatta aagagcgtat cagcagcctt gacgtgcgag acgatattga
      421 gcgccgtgct tatgaaatcg cactcctatc tctggaagta gaaccagatg aacgcgaagc
      481 ttatgaatta ttcatggaaa agcgttttgg tgacttagta gatcgtcgga gagcaaaaaa
      541 cggcgataac gaatacatgg catgggatat gactctcggt tggatcgtct ggcagcaacg
      601 agctggtatc catttctcaa caatgtcaca gcaagaggtg aaataatgga gccatacagc
      661 ctcacactcg atgaggcctg tcattttctc aagatatcca gaccgactgc cattaactgg
      721 atacgcacag ggcgtcttca ggcaacacgc aaagatccca ctaagaataa atctccttac
      781 ctcacaacac gacaagcctg ca
//
LOCUS       DAJVRP010000272          801 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVRP010000272 DAJVRP010000000
VERSION     DAJVRP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.281"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..347
                     /locus_tag="OQN12_004201"
     CDS             <1..347
                     /locus_tag="OQN12_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1656313.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
     gene            702..>801
                     /locus_tag="OQN12_004202"
     CDS             702..>801
                     /locus_tag="OQN12_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein DinI"
                     /protein_id="HCS1656314.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISK"
BASE COUNT          235 a          191 c          177 g          198 t
ORIGIN      
        1 cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta ggtgcaactg
       61 acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt
      121 tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg accaatatcg
      181 gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag
      241 acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc
      301 gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct
      361 accgcaatgg tcacatccta aatgggtgcg gttggggatc accagccttg aagagtatgc
      421 ccgccacttt aactgcgtga tacgggattt ttaaaaatca ctaaagaacg cccaagagca
      481 tgtgttttct ttagaataat caaagcgttg gattgccatg tcatatgaaa atcggcaaag
      541 ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa atatttttgc
      601 cccatgcatg ccccaaaaaa tcactttccc tcactcttga cactgtttat ccatacagtt
      661 aaaaataata ctgtataaaa acacagtgca gagggatttt tatgcgtttt gaaatctgca
      721 tagccaaaga gaaaatgact aaaatgccaa ccggtgctgt ggatgcgtta aaggaagaat
      781 taacccgacg catcagtaaa c
//
LOCUS       DAJVRP010000273          793 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVRP010000273 DAJVRP010000000
VERSION     DAJVRP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.282"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            18..437
                     /gene="rbsD"
                     /locus_tag="OQN12_004203"
     CDS             18..437
                     /gene="rbsD"
                     /locus_tag="OQN12_004203"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS1656315.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            445..>793
                     /locus_tag="OQN12_004204"
     CDS             445..>793
                     /locus_tag="OQN12_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1656316.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          219 a          190 c          197 g          187 t
ORIGIN      
        1 cgctgatgga gaaaaaaatg aaaaaaggca ccgttcttaa ttctgatatt tcatcggtga
       61 tctcccgtct gggacatacc gatacgctgg tggtgtgtga tgctggttta cccatcccca
      121 aaagtacaac gcgtatcgat atggcattaa cccaaggtgt accttctttt atgcaggtgc
      181 tgggcgtcgt cacaaatgaa atgcaggtcg aggctgccat tatcgcggaa gagatcaaac
      241 aacataatcc gcaactccac gaaacgttgc tcactcacct tgagcagctg caaaaacacc
      301 agggaaatac cattgaaatt cgttacacca cgcatgaaca attcaaacaa caaaccgcag
      361 aaagtcaggc ggtaattcgc agcggagaat gttctccgta tgcgaatatc attctctgtg
      421 ctggcgtgac gttctgaggc cgtcatggaa gcattacttc agcttaaagg catcgataaa
      481 gccttcccgg gcgtaaaagc cctctcgggc gcagcgttaa atgtctatcc gggccgtgtg
      541 atggcgctgg tgggcgaaaa cggcgcgggt aaatccacca tgatgaaagt gcttactggt
      601 atctatactc gcgatgccgg cacgctttta tggctgggga aagaaacgac atttaccggg
      661 ccgaaatctt cccaggaagc cgggattggg attatccatc aggaactgaa cctgatcccg
      721 cagttgacca ttgccgaaaa cattttcctc ggtcgtgagt ttgttaatcg ctttggcaaa
      781 attgactgga aaa
//
LOCUS       DAJVRP010000274          768 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVRP010000274 DAJVRP010000000
VERSION     DAJVRP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.283"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..201
                     /locus_tag="OQN12_004205"
     CDS             <1..201
                     /locus_tag="OQN12_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS1656317.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
     gene            204..>768
                     /gene="flhC"
                     /locus_tag="OQN12_004206"
     CDS             204..>768
                     /gene="flhC"
                     /locus_tag="OQN12_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS1656318.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQR"
BASE COUNT          207 a          193 c          186 g          182 t
ORIGIN      
        1 cttccgcaaa tggttaagct ggcagaaacc aatcagctgg tttgtcactt ccgttttgac
       61 agccaccaga cgattactca gttgacgcaa gattcccgcg ttgacgatct ccagcaaatt
      121 cataccggca tcatgctctc aacacgcttg ctgaatgatg ttaatcagcc tgaagaagcg
      181 ctgcgcaaga aaagggcctg atcatgagtg aaaaaagcat tgttcaggaa gcgcgggata
      241 ttcagctggc aatggaattg atcaccctgg gcgctcgttt gcagatgctg gaaagcgaaa
      301 cacagttaag tcgcggacgc ctgataaaac tttataaaga actgcgcgga agcccaccgc
      361 cgaaaggcat gctgccattc tcaaccgact ggtttatgac ctgggaacaa aacgttcatg
      421 cttcgatgtt ctgtaatgca tggcagtttt tactgaaaac cggtttgtgt aatggcgtcg
      481 atgcggtgat caaagcctac cgtttatacc ttgaacagtg cccacaagca gaagaaggac
      541 cactgctggc attaacccgt gccaggacat tggtgcggtt tgttgaaagt ggattactgc
      601 aactttccag ctgcaactgc tgcggcggca attttattac ccacgctcac cagcctgttg
      661 gcagctttgc ctgcagctta tgtcaaccgc catcccgggc agtaaaaaga cgtaaacttt
      721 cccagaatcc tgccgatatt atcccacaac tgctggatga acagagag
//
LOCUS       DAJVRP010000275          755 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVRP010000275 DAJVRP010000000
VERSION     DAJVRP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.284"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..755
                     /locus_tag="OQN12_004207"
                     /pseudo
     CDS             1..755
                     /locus_tag="OQN12_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          186 a          193 c          210 g          166 t
ORIGIN      
        1 ggtccggatt cttccactaa taaagaacag tcagatgctc aggccgagat ccgccgtctc
       61 cagaaagagc tgaaacgggt taccgacgaa cgggacatat taaaaaaagc cgcggcgtac
      121 ttcgcaaagc tgtccgactg aggtacgcct ttatccgtga caactcctgt tgctggcctg
      181 ttcgcctgct ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc tggcttcagc
      241 agccgcattc acaacgccat caggcagacc tgagactgac aggacagatt aaacagttct
      301 ggctggaatc gggatgcgtc tatggttatc gcaaaatcca tctggatctg cgtgacagcg
      361 ggcaacagtg cggagtaaac agagtctgga gactgatgaa acgtgtcgga ataaaggctc
      421 aggtcggata ccgaagcccg cgggcacgta aaggcgaggc cagtatcgtg tcacccaaca
      481 ggctccagcg acagttcaat ccggatgctc ctgatgagcg ttgggtaacg gacataacct
      541 acatcaggac ccacgaaggc tggctgtatc ttgccgttgt tgttgatctg ttctcacgca
      601 aaattatcgg ctggtccatg caatcccgga tgacaaagga cattgtcctg aacgcactgc
      661 tgatggctgt atggcggcgt aatcccgaaa aacaggtgct ggttcattcg gatcagggca
      721 gtcagtacac aagccatgag tggcagtcgt tcctg
//
LOCUS       DAJVRP010000276          750 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVRP010000276 DAJVRP010000000
VERSION     DAJVRP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.285"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
BASE COUNT          208 a          144 c          166 g          232 t
ORIGIN      
        1 tttttcgcgc tggcgctcaa gcatggataa tgcgattgcc tgtagttctt cgttagtcat
       61 tgtctgcacc tggaaagcaa tttaccggag tacctcacac tacggcaaaa atgttttatt
      121 gttgcaactt gcgtaattat tcaggtcagt ttggaacgca ctgtataaac ataaataacc
      181 tgatactgtt gtgtgttttc aaagaaaaca caggtatttt tcacataaac cttccccttt
      241 tcatattcga ccacttaatc aaaaaacggt tttatacttg ttgcccccac tggggttaaa
      301 tgtgagacat gtcgcacttt gtgccatgtg tcctcgctga ttttaggcgc ggttcattca
      361 ttctttctgt tggcataaag tatggtatcg gttgggcgaa tgaaagggac tcgggtcatg
      421 gtcagacaat acctgaggcg ttaattcgtt gaaagagatt gtgaatgggg gaataactga
      481 gtcttaactg ctgtgcgata gcccggacag atagttgctc gtcgaatctg agacgtaatg
      541 attctggcaa ataagtgcga aagccggtga tatctagtgt tgtttgagag ttcatcgttc
      601 gcgtccatta atacagatgg acgcgattgt tgccagacag gataattttc acaagacatc
      661 gcagatagag tacttacgat aattttttat tttatacaac agatgcccac gaaaagaatt
      721 attttcaata gacacctgca ttacacaaag
//
LOCUS       DAJVRP010000277          747 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVRP010000277 DAJVRP010000000
VERSION     DAJVRP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.286"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>747
                     /locus_tag="OQN12_004208"
     CDS             <1..>747
                     /locus_tag="OQN12_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCS1656319.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     LMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT          180 a          177 c          232 g          158 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggctcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgcgctgg gttatcgctc gccacgggaa tatctgcggc agcgggcttg
      721 taatgggtta agtgataaca gatgtct
//
LOCUS       DAJVRP010000278          734 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVRP010000278 DAJVRP010000000
VERSION     DAJVRP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.287"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..475
                     /gene="cobU"
                     /locus_tag="OQN12_004209"
     CDS             <1..475
                     /gene="cobU"
                     /locus_tag="OQN12_004209"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCS1656320.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
     gene            472..>734
                     /locus_tag="OQN12_004210"
     CDS             472..>734
                     /locus_tag="OQN12_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1656321.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADT"
BASE COUNT          160 a          155 c          216 g          203 t
ORIGIN      
        1 ttcacaggtt ctgtatatcg ctacctcgca aatccttgat gatgagatgg ctgcacggat
       61 agaacatcat cggcaaagcc gcccggagca ctggcgcaca gtggagcgct ggcaacatct
      121 tgatgaatta attcatgcag acattaaccc gaatgaggct gtgttgctgg aatgcgttac
      181 cacaatggtg actaatctgt tgtttgatta tggcggcgat aaagaccctg atgaatggga
      241 ttatcaggcg atggaacagg cgattaatgc tgagattcag tcgttgattg ctgcctgcca
      301 acgttgcccc gcaaaggttg tattagtgac taacgaagtg ggaatgggga ttgtgccgga
      361 gagtcgtctg gcacgacatt ttcgtgatat tgccgggcgg gtaaatcagc agttggccgc
      421 tgcggcaaat gaagtatggc tggtggtttc gggtattgga gtaaaaatca aatgagcaaa
      481 ttattttggg cgatgctctc atttattacg cgtctgcctg taccgcgtcg ctggtcccag
      541 ggactggatt tcgagcatta ttctcgcggt attattactt ttcctttgat tggattattg
      601 cttggcgcga ttagcgggct ggtcttcatg gtgctgcagg catggtgtgg cgcaccactg
      661 gcggcactgt ttagcgtact tgtgctggcg ctgatgaccg gggaattcca tctggacggc
      721 ctcgctgata cctg
//
LOCUS       DAJVRP010000279          726 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVRP010000279 DAJVRP010000000
VERSION     DAJVRP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.288"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..264
                     /locus_tag="OQN12_004211"
     CDS             <1..264
                     /locus_tag="OQN12_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1656322.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>726
                     /locus_tag="OQN12_004212"
     CDS             288..>726
                     /locus_tag="OQN12_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1656323.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKN"
BASE COUNT          194 a          167 c          210 g          155 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat attattctga
      361 aatcccgggt aaacaccccg gaagtggtga tgaacactgt tgctgatgtg atgacaggaa
      421 acgatggtga atactgtttc catgcgcgga ctggaaaata tggtgtgtat ctgaaacagg
      481 actggcgcaa cgagtacaac gttggcgaca ttgctgtata tgaggactca aagcccggca
      541 cgctgaatga ctttctgatt gctcctgatg agggcgacct gaaaccggat gtcgtcaaac
      601 gctttgagga aatggtggcg caggcgcagc agagcgccgg ggccgcagcc ggaaacgcac
      661 agcagacggc gcaggatgtg gcggcagccg caggttatgc ccgcgcagca gagcaggcca
      721 aaaatg
//
LOCUS       DAJVRP010000280          711 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVRP010000280 DAJVRP010000000
VERSION     DAJVRP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.289"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            42..>711
                     /locus_tag="OQN12_004213"
     CDS             42..>711
                     /locus_tag="OQN12_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656324.1"
                     /translation="MQGGASLINAISQGLGGPKVLDDVYRPVDRPTDPYAQAGESIGG
                     YLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAGLSIATHGLINGVTRGVRGASNI
                     ISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAAEARVAQGVPMTPATKNPEEVVR
                     TVAAQKRPNLASSLDELDINPQAEVLESAERLNVDSLLPSHFSGNEQYKAVEQAIKSR
                     AGSAL"
BASE COUNT          191 a          168 c          188 g          164 t
ORIGIN      
        1 aacaggctgg tcgtggtctg gtaaacatac catttgacgt attgcagggt ggcgcaagtc
       61 tgattaatgc aatcagtcag gggcttgggg ggccaaaagt attggatgat gtttatcgtc
      121 cagtagacag accgacagac ccctacgcgc aagctggaga gtcaataggc ggttatcttg
      181 ttcctggagc aggtgtagct ggaaacatgg tcattggttc tctcgctgac gcggcgaatc
      241 aacggggtga ttttgccgaa aatgccgcta ttaatgccgg acttagcatt gctacgcatg
      301 gcctgataaa tggcgttacc cgtggtgttc gtggtgcatc aaatataatt agtggcaata
      361 aaacatctgc acagagagcg accactgcgc caacagaaac atcaccattc tccggtgatg
      421 ccgctgcagc aacaaatcct gcggttcatg ccgcagaggc aagagtagca caaggtgtac
      481 caatgacgcc tgcgacgaag aacccagagg aagtcgttcg cacagtagca gcacaaaaaa
      541 ggccaaatct cgcttcatcg cttgatgaac tagatatcaa tcctcaggct gaagttctgg
      601 agtctgctga aaggcttaat gttgattcat tactcccttc acacttttcc gggaacgagc
      661 aatacaaggc agttgagcaa gcaatcaagt cccgtgcggg ttctgctcta c
//
LOCUS       DAJVRP010000281          636 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVRP010000281 DAJVRP010000000
VERSION     DAJVRP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.290"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            88..153
                     /locus_tag="OQN12_004214"
                     /pseudo
     CDS             88..153
                     /locus_tag="OQN12_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="OQN12_004215"
     CDS             381..629
                     /locus_tag="OQN12_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1656325.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DAJVRP010000282          630 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVRP010000282 DAJVRP010000000
VERSION     DAJVRP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.291"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>630
                     /locus_tag="OQN12_004216"
     CDS             <1..>630
                     /locus_tag="OQN12_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCS1656326.1"
                     /translation="QLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
                     VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
                     LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQY"
BASE COUNT          147 a          160 c          178 g          145 t
ORIGIN      
        1 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
       61 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      121 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      181 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      241 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      301 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      361 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      421 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
      481 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
      541 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
      601 ggcaatggag cgcctgccgg cccagtatca
//
LOCUS       DAJVRP010000283          598 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVRP010000283 DAJVRP010000000
VERSION     DAJVRP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.292"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..477
                     /locus_tag="OQN12_004217"
     CDS             <1..477
                     /locus_tag="OQN12_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1656327.1"
                     /translation="PGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAIIAPDANALP
                     AAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISVYVADALLK
                     KGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKENIGKYDF"
     gene            504..>598
                     /locus_tag="OQN12_004218"
     CDS             504..>598
                     /locus_tag="OQN12_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCS1656328.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGAL"
BASE COUNT          181 a          128 c          154 g          135 t
ORIGIN      
        1 ccaggctggg aaattgtcac tacgcagttt ggctataacg atgccactaa atcgttacaa
       61 accgcagaag gaatattaaa agcgtatagc gatctcgacg ccattatcgc ccccgatgcc
      121 aacgccctgc ccgctgccgc acaagccgca gaaaacctga aaaatgacaa agtagcgatt
      181 gtcggattca gtacgccaaa tgtgatgcgc ccgtatgtag agcgcggcac ggtgaaagaa
      241 tttggactgt gggatgtggt tcagcaaggc aaaatttcag tgtatgtcgc ggatgcatta
      301 ttgaaaaaag gatcaatgaa aacgggcgac aagctggata tccagggcgt aggtcaggtt
      361 gaagtctcgc caaacagcgt tcagggctat gactacgaag cggatggtaa tggcatcgta
      421 ctgttaccgg agcgcgtgat attcaacaaa gagaatatcg gcaaatacga tttctgatgt
      481 gcattactta accggagtaa gttatggcag atttagacga tattaaagat ggtaaagatt
      541 ttcgtaccga tcaaccgcaa caaaatatcc cttttaccct gaaaggttgc ggtgcgct
//
LOCUS       DAJVRP010000284          596 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVRP010000284 DAJVRP010000000
VERSION     DAJVRP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.293"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..596
                     /locus_tag="OQN12_004219"
                     /pseudo
     CDS             1..596
                     /locus_tag="OQN12_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          159 a          166 c          159 g          112 t
ORIGIN      
        1 gacaaaaaca gtatcaacca gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag
       61 tgaagccctg aagcttgctg aacgcatcgg tgttactgcc gcagcccgtg aactcagcct
      121 gtatgaatca cagctctaca actggcgcag taaacagcaa aatcagcaga cgtcttctga
      181 acgtgaactg gagatgtcta ccgagattgc acgtctcaaa cgccagctgg cagaacggga
      241 tgaagagctg gctatcctcc aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag
      301 tatgtcttta ttgaaaaaca tcaggctgag ttcagcatca aagcaatgtg ccgcgtgctc
      361 cgggtggccc gcagcggctg gtatacgtgg tgtcagcggc ggacaaggat aagcacgcgt
      421 cagcagttcc gccaacactg cgacagcgtt gtcctcgcgg cttttacccg gtcaaaacag
      481 cgttacggtg ccccacgcct gacggatgaa ctgcgtgctc agggttaccc ctttaacgta
      541 aaaaccgtgg cggcaagcct gcgccgtcag ggactgaggg caaaggcctc ccggaa
//
LOCUS       DAJVRP010000285          561 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVRP010000285 DAJVRP010000000
VERSION     DAJVRP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.294"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>561
                     /locus_tag="OQN12_004220"
     CDS             <1..>561
                     /locus_tag="OQN12_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656329.1"
                     /translation="QFLLTTSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQ
                     DAMNICENVVRTINARMVDVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTEPSF
                     GSEVSDYGIIAHESQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKR
                     FTALSFGCFRHDGENITVVLSRDGDSQ"
BASE COUNT          124 a          146 c          149 g          142 t
ORIGIN      
        1 cagtttttgt tgaccacttc aggtcaggag attgtcgtcg aaccgattga acaggctatt
       61 ctcatctctg agcagactaa gctgtgggac gccaagtcat ccaccggatt cactcagggc
      121 acacttcagc aggacgctat gaacatctgt gagaatgtgg tccgtaccat caacgctcgc
      181 atggtcgacg ttctggaatc ctctaagctg ctgaagaccg tagagcttcc tgtcctgact
      241 ggtagcctga ccgctaaggc agacgctatc atggacgctc tgtatgagaa caccgagcct
      301 tctttcggtt ctgaggtgtc tgactacggt attatcgccc atgagagcca actgaaagca
      361 ctgtcccgtc tggctgctaa acagggcttc tctggtgaag acgctatcgt ggatatgctg
      421 ggtaccgaca tcgcgtatta caacggtgag gacaaaggtg tgttcatgtt ggctaaacgc
      481 ttcacggccc tgagcttcgg ctgcttccgt cacgacggtg agaatattac ggtcgtcctt
      541 tcacgtgatg gcgactctca g
//
LOCUS       DAJVRP010000286          559 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVRP010000286 DAJVRP010000000
VERSION     DAJVRP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.295"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(1..279)
                     /locus_tag="OQN12_004221"
     CDS             complement(1..279)
                     /locus_tag="OQN12_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656330.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OQN12_004222"
     CDS             220..405
                     /locus_tag="OQN12_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656331.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..>559)
                     /gene="hokD"
                     /locus_tag="OQN12_004223"
     CDS             complement(447..>559)
                     /gene="hokD"
                     /locus_tag="OQN12_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1656332.1"
                     /translation="LTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE"
BASE COUNT          116 a          142 c          166 g          135 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaac
//
LOCUS       DAJVRP010000287          548 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVRP010000287 DAJVRP010000000
VERSION     DAJVRP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.296"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(187..366)
                     /locus_tag="OQN12_004224"
                     /pseudo
     CDS             complement(187..366)
                     /locus_tag="OQN12_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            415..>548
                     /locus_tag="OQN12_004225"
     CDS             415..>548
                     /locus_tag="OQN12_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1656333.1"
                     /translation="MIVLILVFRLVIGEQMIDVLGPEKRRRRTTQEKIAIVQQSFEPG
                     "
BASE COUNT          156 a          101 c          140 g          151 t
ORIGIN      
        1 ttgaagagta gctggtagat gttattgtgc cagcaggggc aggggaatgt gccgtatcga
       61 tatgctttgg caattgcgtg tgccgcagat ggagatgaaa tgccaataaa aaagcctgag
      121 ttcgatgagg gacgaactca ggctggtgtt atgttttaca gtaacacgcc tcggcgcgtt
      181 ggctgattag aactggtaga cgataccaac tgccagttgg tcgtcggtgt tcagtccatg
      241 agcgagaaca taatcactct tatcaagcag gttgatctga taagcggtat atatgttcat
      301 attcttattg aagtaatacc aggtaccgat ttcaatatag ttcagacgat cagaatcttt
      361 gtaacctgta gactggcccc ctgaatctcc agacaaccaa tatcacttat ttaagtgata
      421 gtcttaatac tagtttttag actagtcatt ggagagcaga tgattgatgt cttaggaccg
      481 gagaaacgca gacggcgtac tacacaggaa aagatcgcta ttgttcagca gagttttgaa
      541 ccgggaat
//
LOCUS       DAJVRP010000288          545 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVRP010000288 DAJVRP010000000
VERSION     DAJVRP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.297"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
BASE COUNT          180 a          107 c           95 g          163 t
ORIGIN      
        1 cttatcaagg gataattaat acgactcact atagggagaa gggacacaaa aagttttctc
       61 ttctaggaca actaaccgta acttatcgca tcctttcagg ggcgggatga ccaattacag
      121 acaccaagcg ttctacttaa agtgaatgct tcctgtgtgc tctgggtcag tcgataagtg
      181 aatagcaata agacacaatc atcttaatta tggtctctag tccctacagg ggcttaactt
      241 aaagttaact taaagacaca attaaagatt aacttatagt attacttaaa gttaacttaa
      301 agacacaatt agagattaac tttaagtatt aaataaaaga tttaattaaa gaccaattaa
      361 agattaactc taagggaata ccttaagggg tgatgatatt tataataatc aatcaccaaa
      421 cctataggtc ttctctaggg tctctctata ggtctctcta gggtctcctc taggcattac
      481 ccccagaagc taactctatg gccctctatg gtcctctatg gccctgtacg tcattcttta
      541 ggatg
//
LOCUS       DAJVRP010000289          535 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVRP010000289 DAJVRP010000000
VERSION     DAJVRP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.298"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
BASE COUNT          174 a           78 c          107 g          176 t
ORIGIN      
        1 ttatatgaat atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa
       61 atagccctgc tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg
      121 tatctgtcag cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag
      181 caatgtatct tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc
      241 ttacatcacg actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc
      301 acaccgtaat gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc
      361 tcttattttc cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga
      421 tctcatttcg agtattgaga aatttgtgat ttttaaattt ttcaataagc cctgtaaggg
      481 attggggttt tatgagaaat aaagagacgt attgtctttg tttaaacggt acgta
//
LOCUS       DAJVRP010000290          531 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVRP010000290 DAJVRP010000000
VERSION     DAJVRP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.299"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..272
                     /locus_tag="OQN12_004226"
     CDS             <1..272
                     /locus_tag="OQN12_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCS1656334.1"
                     /translation="KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSR
                     YSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(380..>531)
                     /locus_tag="OQN12_004227"
     CDS             complement(380..>531)
                     /locus_tag="OQN12_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656335.1"
                     /translation="DPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTTTKML
                     FMCRE"
BASE COUNT          175 a          123 c           95 g          138 t
ORIGIN      
        1 taaagggata cgattacgat acatatacct tctatgccaa aaaaggtcag aaagtgcatg
       61 tgagtatctc taatgaaggc gctgatactt atctcttcgg gccaggaatc gatgattctg
      121 ttgatttatc cagatattcc ccagaactcg atagtcacgg acaatactca ctccctgctt
      181 ctggcaaata tgagctgcga gtgctgcaaa ctcgaaacga cgcccgtaaa aacaaaacta
      241 aaaaatataa cgtcgatatt caaattaagt aaatgtcaac cgacggggac tatccccgtc
      301 atagacgtta acgccccact cctctctgtc gctcaagagg ttaatgacga atagcagaga
      361 acataatgta aacaaggcat cactcacgac acataaataa catctttgtg gtggtttgat
      421 tgatttttcc actccgctta ccatctacac caaaataacg atcatcacga cataattttt
      481 ctttaacatc gcaaaataca ccgttagcga atgtaaattc gctgggatcg t
//
LOCUS       DAJVRP010000291          524 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVRP010000291 DAJVRP010000000
VERSION     DAJVRP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.300"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..119)
                     /gene="csgC"
                     /locus_tag="OQN12_004228"
     CDS             complement(<1..119)
                     /gene="csgC"
                     /locus_tag="OQN12_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCS1656336.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLC"
     gene            complement(178..>524)
                     /gene="csgA"
                     /locus_tag="OQN12_004229"
     CDS             complement(178..>524)
                     /gene="csgA"
                     /locus_tag="OQN12_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCS1656337.1"
                     /translation="NSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADV
                     GQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQ
                     VGFGNNATAHQY"
BASE COUNT          139 a          122 c          126 g          137 t
ORIGIN      
        1 ctgcacagac aagattgagt aagagtaact tcaggaataa tggtgtacat atccccttgc
       61 tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa cgcattcata
      121 ttcttctccc gaaaaaaaac agggcttgcg ccctgtttct gtaatacaaa tgatgtatta
      181 atactgatga gcggtcgcgt tgttaccaaa gccaacctga gtcacgttga cggaggagtt
      241 cgatgcagtc tggtcaactg cagcaccgtt gccaccaccg aactgtttaa cagtcatttc
      301 agaatttttg ccgttccact gatcaagagt ggcgctgtta ccgaagccac gttgggtcag
      361 atcgattgag ctgtcatctg agccctgacc aacatctgca ccattaccac cgccatgctg
      421 ggtaatagtc aagtcagagt tacgggcatc agtttgcaga gcaagtgcag agttaccgcc
      481 accgtactgg taaatgttca gctctgaatt tgggccgcta ttat
//
LOCUS       DAJVRP010000292          524 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVRP010000292 DAJVRP010000000
VERSION     DAJVRP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.301"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>524
                     /locus_tag="OQN12_004230"
     CDS             <1..>524
                     /locus_tag="OQN12_004230"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1656338.1"
                     /translation="VESIKNDVEWLGFHWSGNVRYSSDYFDQLHAYAVELINKGLAYV
                     DELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEKMRAGGFEEGKACLRAKIDMA
                     SPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNR
                     RLYDWVLDNITIPV"
BASE COUNT          124 a          140 c          138 g          122 t
ORIGIN      
        1 gtcgagtcga ttaaaaacga cgttgaatgg ttaggttttc actggtctgg taacgtccgt
       61 tactcctccg attattttga tcagctccac gcctatgctg ttgaactgat caacaaaggc
      121 ctggcgtatg ttgacgaact gacgccggaa cagatccgcg aataccgcgg caccctgacg
      181 cagccgggta aaaacagccc gtaccgcgat cgcagcgttg aagagaacct ggcgctgttc
      241 gaaaaaatgc gtgccggtgg ttttgaagaa ggtaaagcct gcctgcgtgc gaaaatcgac
      301 atggcgtcgc cgtttatcgt gatgcgcgat ccggtgctgt accgcattaa gtttgctgaa
      361 caccaccaga ctggcaacaa gtggtgcatc tacccgatgt acgacttcac ccactgcatc
      421 agcgatgcgc tggaaggtat tacgcactct ctgtgtacgc ttgagttcca ggacaaccgt
      481 cgcctgtacg actgggtgct ggacaacatc accattcctg ttca
//
LOCUS       DAJVRP010000293          520 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVRP010000293 DAJVRP010000000
VERSION     DAJVRP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.302"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(103..>520)
                     /locus_tag="OQN12_004231"
     CDS             complement(103..>520)
                     /locus_tag="OQN12_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1656339.1"
                     /translation="PGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAM
                     VFDGLRLMKNGYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          147 a          150 c          100 g          123 t
ORIGIN      
        1 agtgagtcgt attaattatc ccttgacaag tagagaaaac catgagtcaa aaacatgacc
       61 acacaggtct gtagtaagtg tcatcctaga ctatggtctc ctctaggtct ccttataaac
      121 atcagccatc acattgacca gcctatcaac ctcgacctct ttagcataac ggtcgtacgt
      181 aatagaccct gaactatgcc ccatgatcgc ctgtgcatac gccaactcga ccccatgttg
      241 ttttaactgg gtcgcaactg tatgtctaaa cgagtgaaac gctaagccgc tacggtctac
      301 gaggacttta ggcaacaacg tccgattaaa ccatttactc acctgctcac cgtaaccgtt
      361 cttcatgaga cgcagaccat cgaacaccat agcgtcatca ccgtcagcgc ctctacgggt
      421 ctctacaagc gccataaagt cagccaatat aaaaccatag gccccatcga ccagaggcac
      481 acagcggtca ctatgagcgt tcttgatgct cttaccgggt
//
LOCUS       DAJVRP010000294          520 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVRP010000294 DAJVRP010000000
VERSION     DAJVRP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.303"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>520
                     /locus_tag="OQN12_004232"
     CDS             <1..>520
                     /locus_tag="OQN12_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1656340.1"
                     /translation="HDKDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKAR
                     SVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENA
                     LRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQ
                     VHELLPWNFSSVK"
BASE COUNT          150 a          111 c          159 g          100 t
ORIGIN      
        1 ccatgacaag gatgtgcgcc gtccgacaga aatgactcag gaagcgctca gacggatagc
       61 agagttatac gacatagaag cggagatacg tggcagtccg gcagaggaac ggcttgcagt
      121 cagaaaagcc agaagcgtcc agttgatgca gtcgttgtac gactggatac agttgcagag
      181 gaaaacgctg tcgaaacatg cggagatggc gaaggcgttc gactatatcc tgaatcactg
      241 gaatgcgctg aacgagttct gtcgtgacgg ctgggtggaa atagacaaca acatcggtga
      301 aaacgcgtta cgatcggtgg cggttggaag aaaaaattat ctctttttcg gctcagacaa
      361 gggaggagaa agtgcggcga tcatctacag tctgctggtc acctgcaaac agaacgaagt
      421 ggagccggag gactggttgc gcgaagtgat cgagaagctc aatgactggc cgtcgaacca
      481 agtgcatgaa ctgctgccct ggaacttctc gtctgtaaaa
//
LOCUS       DAJVRP010000295          518 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVRP010000295 DAJVRP010000000
VERSION     DAJVRP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.304"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            108..>518
                     /locus_tag="OQN12_004233"
     CDS             108..>518
                     /locus_tag="OQN12_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCS1656341.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQA"
BASE COUNT          105 a          143 c          155 g          115 t
ORIGIN      
        1 acggagcggg aatttgcgac cattcttgcc ggtatcagcg ccggcggtat gtactgcccg
       61 gagtgtggca cggttcactg gccggacgga gtcgccccgc ccttctgatg ctcccccgtt
      121 ttgccgatat ttttcagcag ggaaaccgct ggcttaactg gctggagaaa caaccggaag
      181 gggcagtccg tccggtggta attgaatcag tgacaaaaat catggcctgc gggaccacac
      241 tgatgggcta cacgcagtgg tgttgttcat caccggactg ttgccataca aaaaaggtct
      301 gcttccggtg taaaagccgc tcctgcccgc actgcggggt gaaggctggc gcacagtgga
      361 tacagtatct gctgagcctg gtccccgact gcccgtggca gcatattgtg ttcacacttc
      421 cctgccagta ctggtccctg gtgttccaca accggtggtt actggcagag atgagccgca
      481 ttgcagcgga tgtgatactg gaaatctgcc atcaggca
//
LOCUS       DAJVRP010000296          514 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVRP010000296 DAJVRP010000000
VERSION     DAJVRP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.305"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>514
                     /locus_tag="OQN12_004234"
     CDS             <1..>514
                     /locus_tag="OQN12_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1656342.1"
                     /translation="LEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAA
                     DIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLT
                     SMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKAL
                     KRALFLSAFAA"
BASE COUNT           97 a          173 c          140 g          104 t
ORIGIN      
        1 acttgagcac ccggcggtac tcgatcttct ccagcgatat ccctcaccag aaaaactcgc
       61 ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc gtctgggtaa
      121 acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg ttcccggcac
      181 gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc tgcgtaagca
      241 aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc tttacccggt
      301 cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca ccgaggtcgc
      361 ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg cgccggtaac
      421 tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta ataaagctct
      481 caaacgggcg ttgttcctgt cggccttcgc cgcg
//
LOCUS       DAJVRP010000297          511 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVRP010000297 DAJVRP010000000
VERSION     DAJVRP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.306"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..>511)
                     /gene="ipaH9.8"
                     /locus_tag="OQN12_004235"
     CDS             complement(<1..>511)
                     /gene="ipaH9.8"
                     /locus_tag="OQN12_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1656343.1"
                     /translation="DNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQ
                     TMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVL
                     KRTEADRWAQAEEQKYEMLENEYPQRVADRLKASGLSDDADAEREAGAQVMRETEQQI
                     YRQLTDEVLA"
BASE COUNT          104 a          160 c          119 g          128 t
ORIGIN      
        1 gccagtacct cgtcagtcag ctgacggtaa atctgctgtt cagtctcacg catcacctgt
       61 gctcctgctt ccctctccgc atccgcatca tcgctcagac cggatgcttt cagccggtca
      121 gccaccctct gagggtactc attctccagc atctcatact tctgctcttc tgcctgcgcc
      181 cagcggtcag cttccgtacg cttcagtaca gcatgccatg gtccccagag ggagaaccag
      241 tccgtaaatt cattctcttc acggcttctg accatggctt cggcagtgcg gaggtcattt
      301 gctgtcactc ccgacacgcc atagaaacgc atttccttca cggcagtgga gagctgaagt
      361 ttctctgcga gcatggtctg gaaggccagg tagacttcta tctcatccac aaaatggaga
      421 gttctgactt tatcccgggc aatatcctcc agaatttcga ggcggaacat ttccctgccc
      481 agggagagca gagcgccggt atcattatcg a
//
LOCUS       DAJVRP010000298          500 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVRP010000298 DAJVRP010000000
VERSION     DAJVRP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.307"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>500
                     /locus_tag="OQN12_004236"
     CDS             <1..>500
                     /locus_tag="OQN12_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCS1656344.1"
                     /translation="RDAGTSASEAATNATDAAGSARAASTSAGSAASSASSASASKDE
                     ATKQASAAKGSATTASSKATESAGSATAAAQSKSAAESAAMRAETAAKRAEDIADVVS
                     LEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAPLNSPALTGTPTTPT
                     APKGTN"
BASE COUNT          138 a          139 c          157 g           66 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata
//
LOCUS       DAJVRP010000299          492 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVRP010000299 DAJVRP010000000
VERSION     DAJVRP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.308"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..201
                     /locus_tag="OQN12_004237"
                     /pseudo
     CDS             1..201
                     /locus_tag="OQN12_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            301..>492
                     /locus_tag="OQN12_004238"
     CDS             301..>492
                     /locus_tag="OQN12_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656345.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDL"
BASE COUNT          170 a           99 c           97 g          126 t
ORIGIN      
        1 tattggctac gtaattaaaa cagaaaataa agaaacccca ttcactgtag ttgatatcga
       61 cggtctatca ggaaacttta aaactcttgt tgaaggtgcc acaaaaatgt gcctagttta
      121 catcggaaat aatctgcccg cagaaaaaaa gccgaatttc tggcaaatct gattgcaatg
      181 aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca gtgtgcaggc ctgagtgaag
      241 aacctgggac atttattcat cactcgcagt aattttaatc tgagttgagg ttaaaaaaca
      301 atgagcacaa aaccactctt cctgttacgg aaagcgaaaa aatcatccgg tgaacctgac
      361 gtcgtcctgt gggcaagcga cgattttgaa tcgaccagca ccactctgga ctatctgctc
      421 gttaagtcag gtaaaaaact gagcaactat tttaaagctg ttgccacaaa ttttcctgtc
      481 gttaatgacc tt
//
LOCUS       DAJVRP010000300          480 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVRP010000300 DAJVRP010000000
VERSION     DAJVRP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.309"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(52..480)
                     /locus_tag="OQN12_004239"
                     /pseudo
     CDS             complement(52..480)
                     /locus_tag="OQN12_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="The RecE (also known as exonuclease VIII) pathway
                     is an alternative pathway for the initiation of homologous
                     recombination in Escherichia coli. The RecE pathway is
                     activated in recB, recC sbcA (suppressors of recB and
                     recC) mutants. Activation of the RecE pathway results in
                     the production of at least two phage proteins relevant for
                     recombinational repair. RecE is a duplex DNA-specific
                     exonuclease which produces 3' overhangs in duplex DNA by
                     preferentially degrading the 5'-ending strands.
                     Recombinational repair of E.coli chromosomal DNA involves
                     as well other proteins: RecA, RecF, RecJ, RecO, RecR, and
                     RuvC.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
BASE COUNT           99 a          122 c          124 g          135 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagccttaat
       61 tgcatcagtc aggcggagca gggcggcatt atccagagtt gtcggttctg ccatgccgcg
      121 tatggccaac agcagattct ggacatagct gttttcctga tccatctcaa gagcagtaat
      181 gtgtttgcgt tgttcacggg tggcatgatg caggtatttc cgatccccgg cagcatacgt
      241 aaaaatgtgc agaagacgct gtgtgaaccg caaagtggct acagagactt cgcaatcctg
      301 gcaatcctcg tgggcgtctg cctgcgcgtt ttcttcctgg cctcccgcca gttctttggt
      361 ttcttgagca ttatcctggt ggtgaacgtc gtctggcgct gctcccggtt ttagttccca
      421 tgtcatggag tctttgctga gttgatagcg ttcactccag gtaaaatcga tctcaccttc
//
LOCUS       DAJVRP010000301          479 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVRP010000301 DAJVRP010000000
VERSION     DAJVRP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.310"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
BASE COUNT          135 a           98 c           89 g          157 t
ORIGIN      
        1 aacaggatgc tctgactcgc aattcatcca gcagtttatc atttcccata atgtcactta
       61 ccctcatcgg taaaaaaatt gcctttcgac cattacgata cattattgat tttggggttt
      121 cagggaagta atccatttcg actataactg acaggtcatc acgacgtatg actgcgtatt
      181 tgctactaaa tagtttcttt attttttcca cgatgccccc aggtttataa gtacaaacgg
      241 ttatatccac atagagacaa aaatattaat ctgataaata tttatttcac gccgtatatt
      301 tgattgttta atgtgcaagt acaatgactt ttattttttg ttgtgtatat aatcaaatat
      361 atggttattt ttcaccctgc gtattcagca cgcaacaaaa aacccgccga agcgggttta
      421 gtgcgggtgc gttgaggatg cctgacacat gagaggtggc gagggatttc tcccccgcc
//
LOCUS       DAJVRP010000302          479 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVRP010000302 DAJVRP010000000
VERSION     DAJVRP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.311"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            209..>479
                     /locus_tag="OQN12_004240"
     CDS             209..>479
                     /locus_tag="OQN12_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1656346.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPG"
BASE COUNT          109 a          126 c          130 g          114 t
ORIGIN      
        1 ttataatggg gtgttagcca gccagacccg gcatgattac tgcccccagt cgtccatgat
       61 ccgggggggg atgtcaccgg gtctggtggg gcgctggtaa ccgctaatag gggtcaggtc
      121 aggcactttt gccgggaccg tctgtaacgt ggatgccggt acctgctccc cgtggttatc
      181 tggttaaccc atatacaagg gagacagaat gaccgaatcc agcgattacg aatccgtcca
      241 ggtctttatc ggcgttgatg tcggtaaaga tacgcatcac gctgttgcca ttaatcgttc
      301 aggtaaacgc ctgttcgata aagcattacc caacgacgaa aacaaactca ggtcgctaat
      361 atctgacctg aaacaacatg gtcagatact gctggttgtt gatcagccag ctaccatcgg
      421 tgcgttacct gtcgccgttg cccgctcaga aggagtcctt gtcggatacc tccctggac
//
LOCUS       DAJVRP010000303          460 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVRP010000303 DAJVRP010000000
VERSION     DAJVRP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.312"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(47..460)
                     /locus_tag="OQN12_004241"
                     /pseudo
     CDS             complement(47..460)
                     /locus_tag="OQN12_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          132 a          113 c           89 g          126 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcaggtc tgcatagcat
       61 tatcaacatt cggatgtgca actacagcta atgcaacatc atcccagctt gcaatagtgc
      121 tatcagcaac accatctccg tcagtatcta cggtgacaaa gttagattta ccactataat
      181 cgctggcatt acctgtatta ccgaaccaac cagtagtacc ttcatcagtc catgaaccag
      241 aggttacggt agaatcttta acagtgatgg tgttattaaa cacttcgctg ctgtttacgc
      301 cagtgctgac agtgttgtcg tcaatatctt cccactcata accctgagtc aacgtaatac
      361 ccgcaacgtg agagttatta gtgatggaca gatcaacttc ctgatccaga gtaatagccg
      421 taccaatgtt gtaaacgtct acaacatgag tatcagcggc
//
LOCUS       DAJVRP010000304          457 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVRP010000304 DAJVRP010000000
VERSION     DAJVRP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.313"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..397
                     /locus_tag="OQN12_004242"
     CDS             <1..397
                     /locus_tag="OQN12_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656347.1"
                     /translation="NAAFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQV
                     IPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTG
                     RNSADNVDGVYYRPLQKLINGTWYNVASI"
BASE COUNT          132 a           92 c          117 g          116 t
ORIGIN      
        1 aaatgcagcg ttttatttgt ggggtaatcc atccaggcct gttgttgcag agcttggtga
       61 tgattcaggc tggcattttt tcagccagag aaacccggat aacagcatag tgttcactgt
      121 taacggacag gtaattccgt taaattacgg aaacttcgat gcccgctata aatatcgaac
      181 agagggggta caggatgtac ggtatggcca tgaaatgtat tacagccccg gcagtaacac
      241 cgtttcgtgg agattttgcg caccttcggg acacgggctg tcagggatgg cgatatcgga
      301 taccggccgt aactcagcgg ataacgttga cggtgtgtat taccgaccac tgcaaaaact
      361 gattaatggc acctggtata acgtagcgag tatttaacaa tgttgcattt aaaaaatatt
      421 actgcgggta atccgaaaac agcagaacaa tatcaga
//
LOCUS       DAJVRP010000305          451 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVRP010000305 DAJVRP010000000
VERSION     DAJVRP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.314"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..90
                     /locus_tag="OQN12_004243"
     CDS             <1..90
                     /locus_tag="OQN12_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1656348.1"
                     /translation="RTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          141 a          114 c           90 g          106 t
ORIGIN      
        1 cgcaccgccg ataaagattc agctaaaact tttgttcagg aaactctgaa agatacctgg
       61 gagtctgctg acgagtggtt tgttcactaa ttagcacgta aaatcagtaa cggctggaaa
      121 tcattcaata ctcgcactat cggaagttca ccagccagcc gcagcacgtt cttgcatacg
      181 acgtgtctgc ggtctcaact ccgcccgccc ccgccaatca tgattgggca gtaccagtac
      241 agcactagca aagacaggaa attagcagtc tcagcaaaac accgaccaga cggtgagaag
      301 acaaaaagat acgcaaagat gccgcggttc cagaagaata aaaaaccgct aatggacagc
      361 ctccttaaca cagtttttta tatacatgat tcactgatac cagtggcatt aatagctatt
      421 atatagcttc agctcctttt ttcatacctt a
//
LOCUS       DAJVRP010000306          444 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVRP010000306 DAJVRP010000000
VERSION     DAJVRP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.315"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..>444)
                     /locus_tag="OQN12_004244"
     CDS             complement(<1..>444)
                     /locus_tag="OQN12_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HCS1656349.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          101 a          113 c           86 g          144 t
ORIGIN      
        1 catgttcgaa caccactcca acatctgttt gaacacctgt taaatttctc tcttgttttg
       61 tttgaacatc tgcttccttt ctgcttcttc tggcctgaac agatgctttt cctgcggctg
      121 attttttggt taatttttct ctgacagatg ccagatcttc ctcaatccga agatgcatcc
      181 attcgtcgcc gttatcacaa aaaaactccc gcaaggatgg ttcaacatca acccatcgct
      241 cgttagtcag acgggcaatt tttgccaacc tgtttttggg tattggcttt cctgtttgcc
      301 agtaattgaa catcagcaac aaatacgcgc cgtgctcctc tgctgacaaa tgcatggtgt
      361 cagccaggta atcagctatg tacagttgca tgtatggtaa tgcggccata attgccccgt
      421 atgatgctac ccggttgctt agaa
//
LOCUS       DAJVRP010000307          434 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVRP010000307 DAJVRP010000000
VERSION     DAJVRP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.316"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..373)
                     /locus_tag="OQN12_004245"
     CDS             complement(<1..373)
                     /locus_tag="OQN12_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656350.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSL"
BASE COUNT          133 a          104 c           91 g          106 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctga
//
LOCUS       DAJVRP010000308          434 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVRP010000308 DAJVRP010000000
VERSION     DAJVRP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.317"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>434
                     /locus_tag="OQN12_004246"
     CDS             <1..>434
                     /locus_tag="OQN12_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCS1656351.1"
                     /translation="FMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVGNAANLLI"
BASE COUNT          115 a          104 c          112 g          103 t
ORIGIN      
        1 gctttatgtg gaaagagccg ggaacaagct gcatccatct ggaaaaaaca catctgatta
       61 tcaagctgtt acggtcgatt attgatgacg tagcaccagg tacagtgatc attaccgaaa
      121 ccaatgttcc gcacaaagac aacattgctt actttggcaa cggtgatgac gaagcgcata
      181 tggtgtacca gttctcgctg ccgccgctgg tgttacatgc ggtgcaaaaa cagaacgtcg
      241 aggcgctgtg ccagtgggcg caaagtctga gcttgccctc cagtaaaacg acctggttta
      301 acttcctcgc ctctcacgat ggcatcgggt tgaacccact gcgtggcttg cttccagaaa
      361 gcgaaatatt agcgctggtc gaggcattac agcaggaagg tgcattagta ggtaatgctg
      421 ccaacttact gatt
//
LOCUS       DAJVRP010000309          424 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVRP010000309 DAJVRP010000000
VERSION     DAJVRP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.318"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..402)
                     /locus_tag="OQN12_004247"
     CDS             complement(<1..402)
                     /locus_tag="OQN12_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCS1656352.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEY"
BASE COUNT          115 a          105 c           92 g          112 t
ORIGIN      
        1 gtattccgtc agtgcgtggg cttcttccag ccacaacata tcgatcccct ccagtgactt
       61 aatttcttcg atgttgcgcc ataatccata aaaaacaaat tccgagccag taacccggtt
      121 aatgattttg ttgttcagaa tacggaaacg gtgccgcaga ccaaacctgt ctatctgaat
      181 tttgagcagg gtatacaccg actcttcgat tttattctgg atctgacggg cacaacaaaa
      241 acgcagggtg tatttattcg acagaaatat ggcaatgcca gcggcatccc atgattttga
      301 cgatgaccga ccaccataaa gcactttgtt acgtgcccgc atagtccaga aactacgtaa
      361 agccggattc agcgtcggtt tggatgtcag agtagaagtc attgaggtca cgctctccat
      421 tgcc
//
LOCUS       DAJVRP010000310          412 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVRP010000310 DAJVRP010000000
VERSION     DAJVRP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.319"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..412
                     /locus_tag="OQN12_004248"
                     /pseudo
     CDS             1..412
                     /locus_tag="OQN12_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          105 a          106 c          120 g           81 t
ORIGIN      
        1 gccggagaaa cgcagacggc gtaccacaca ggaaaagatc gcaattgttc agcagagctt
       61 tgaaccgggg atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagccagtt
      121 atttctctgg cgtaagcaat accaggaagg aagtcttact gctgtggccg ccggagaaca
      181 ggttgttcct gcctctgaac ttgctgccgc catgaagcag attaaagaac tccagcgcct
      241 gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat atggacgggc
      301 aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag cttagtcagc
      361 cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac cg
//
LOCUS       DAJVRP010000311          411 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVRP010000311 DAJVRP010000000
VERSION     DAJVRP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.320"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(196..>411)
                     /locus_tag="OQN12_004249"
     CDS             complement(196..>411)
                     /locus_tag="OQN12_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1656353.1"
                     /translation="LEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMR
                     FTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT          101 a          130 c           79 g          101 t
ORIGIN      
        1 actctcgatc gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg
       61 tttcttcaac accacttttg ctccatatga acggaaccga cgattaaact ggatggctct
      121 gattgattca gggtatgaat ggcggttttt tgctccgttt ccctcaaaat ggacgcaact
      181 tcccctctgc ggctctcagc cgcaccaccg catccgggcc agcggctcat gcatcaggac
      241 ctgctctgcc agacggtagc cccgcttcag ccccgtaaaa cgcatctgac tcccgcacag
      301 cacgcacttc agcgggtcaa ccttcagtaa cctctgatac atccctctcc aggtgatttg
      361 catcgccgtt tttctcactg tctccgttat gatgtacacc acttcttcca g
//
LOCUS       DAJVRP010000312          402 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVRP010000312 DAJVRP010000000
VERSION     DAJVRP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.321"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..334
                     /locus_tag="OQN12_004250"
                     /pseudo
     CDS             1..334
                     /locus_tag="OQN12_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          133 a           89 c           98 g           82 t
ORIGIN      
        1 aaaagggaaa cctgttccag aatacacgcc aaaaccagcg tccgttgcaa cgaaggctac
       61 gcatctttca tttataaagg ccctactaag agccgcagag cgtgaatgga aaatgctgga
      121 taaggcacca attattaaag tgcctcaacc aaagaataaa cggatccgct ggctggagcc
      181 ccatgaagca caaaggctga ttgatgaatg tccggagcca ttaaagtctg ttgttgaatt
      241 tgcactggca acaggcttaa gacgctcgaa catcatcaac cttgaatggc aacaaataga
      301 tatgcagcgc cgggtggcat ggataaaccc ggaagtgagg tagcctgagt ttaacggaca
      361 ctccttcctg aaatagaatg gcatcagaag gagctaataa tg
//
LOCUS       DAJVRP010000313          396 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVRP010000313 DAJVRP010000000
VERSION     DAJVRP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.322"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..178)
                     /locus_tag="OQN12_004251"
     CDS             complement(<1..178)
                     /locus_tag="OQN12_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1656354.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
BASE COUNT           95 a           95 c           95 g          111 t
ORIGIN      
        1 ccctgggtac tttcttcatg aaactgcggt gttcgagttt gcgatatttc ttacagggga
       61 acgcaagccc ctccgcctcc gctttggatt tccgatcagc attaatgtaa tccaccatct
      121 caagactgct catggtcggg tattcgccag aaggaagaac ggttaatgat ttgttcatgt
      181 cggtatttcc tttagaaagt tgagcctgtt cgcacagaaa agccgtcccc gagaggtcct
      241 cacctatacg gcagttctca ggctcagctt tctgaaagac tcgggattgt tacgcgctgc
      301 gatgcgcggt ttactgcgga catataaaag ccccgcaagt gcgaggctca ttaaatggac
      361 tttgtgattt gcaaaaaaat ttatttcagg cactga
//
LOCUS       DAJVRP010000314          383 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVRP010000314 DAJVRP010000000
VERSION     DAJVRP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.323"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..322)
                     /locus_tag="OQN12_004252"
     CDS             complement(<1..322)
                     /locus_tag="OQN12_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1656355.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FKVRL"
BASE COUNT          102 a           79 c           99 g          103 t
ORIGIN      
        1 ccagacggac cttaaattca cggctatagc tggttcgccg ttcttttcgc catgagcctt
       61 ctctgatttg aggctctgtt aattccttct ttctgttggc ataaaggatg gcgtcaagtt
      121 gagcgaatga aactgaatcg ggcaatggcc atgcgatacc ggatgcaaga aatcgctgaa
      181 aaagcgtatg tattgtggaa tgactgagac ccagacgctg agcgatggcc cggatggtca
      241 gtttatcttc aaatcttaaa cgcagggcat caggcaaata agaacggaag cagggaatat
      301 cttttgttgt ctgggaattc atcgttcgtg tccatcaata tagatgggcg cgattgttgc
      361 cagacaggac aattttcaca aga
//
LOCUS       DAJVRP010000315          375 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVRP010000315 DAJVRP010000000
VERSION     DAJVRP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.324"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..269
                     /locus_tag="OQN12_004253"
     CDS             <1..269
                     /locus_tag="OQN12_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1656356.1"
                     /translation="LLPVHLTVTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDF
                     QLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
BASE COUNT           94 a           90 c           97 g           94 t
ORIGIN      
        1 cgctattgcc agtacacctc accgtgacca taccgcgtgg gcagctgaaa aacctgaagg
       61 cgagctacac gttaacggaa ccgcagctta ccgcaccgct gaaaaaaggt caggttgtcg
      121 ggaccattga tttccagctt aacggtaaat ccattgagca gcgtccgctg atcgtgatgg
      181 aaaatgtgga agagggcgga ttctttggta ggatgtggga tttcgtgatg atgaaattcc
      241 atcagtggtt cggcagctgg ttctcttaat cttctgataa ccggatggcg cgaaacgtca
      301 tccggttata cgtcattaat acatcaactt aatacggtgc gtctgcgcgt acttcacata
      361 ctcatcttcc gggca
//
LOCUS       DAJVRP010000316          370 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVRP010000316 DAJVRP010000000
VERSION     DAJVRP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.325"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            210..>370
                     /locus_tag="OQN12_004254"
     CDS             210..>370
                     /locus_tag="OQN12_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1656357.1"
                     /translation="MSQQPQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLR
                     DIRKMLEEL"
BASE COUNT          107 a           96 c           80 g           87 t
ORIGIN      
        1 tatagcctga tgcctagaga gaccctagag tggctcctag agagaccctc ccagtagctc
       61 cgcacagcta catcgtcatc atcatagtat tcaatctcaa agtcattctg tttgcatcct
      121 gttgtcctgc tggtctccct tcgcttgggg actttgagta ggtcagtggg ggtgcgactt
      181 cagggtgcaa acaaaaagga aatcaagcga tgtctcaaca accacaacaa ccacaacaac
      241 cacaacaaga aatcagcatc attaacctcg accagcttgt gtccatgacc tcagttgaaa
      301 ttgctgagct gacaggcaag gagcaccgaa atgtccttcg tgatattcgt aagatgctcg
      361 aagaacttaa
//
LOCUS       DAJVRP010000317          365 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVRP010000317 DAJVRP010000000
VERSION     DAJVRP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.326"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>365
                     /locus_tag="OQN12_004255"
     CDS             <1..>365
                     /locus_tag="OQN12_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656358.1"
                     /translation="ENDSILAWIRNTDWAKIGFKNDSDADTDSYMWFETGDNGNEYFK
                     WRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIGTTNALGGNSIAFGDNDTGLKQN
                     GDGLLDVYANGQHVFRFQNG"
BASE COUNT          102 a           68 c           97 g           98 t
ORIGIN      
        1 gaaaacgact caatccttgc ctggattcgg aatactgact gggcgaagat tggatttaaa
       61 aatgattcgg atgcagacac cgattcctac atgtggtttg aaacaggtga caacggcaat
      121 gagtatttca aatggagaca ccgcctcgct ggcggccagc tcaaagagct gatgaatctt
      181 aaatgggatt cactaaatat tctggttaat gccgtcatta atggttgcct tggcattggt
      241 acgacgaatg cgttaggtgg aaattcaatt gctttcgggg ataacgatac cggcctgaaa
      301 cagaacggcg atggtctgct ggatgtttat gcgaatggac agcatgtatt tcgttttcag
      361 aatgg
//
LOCUS       DAJVRP010000318          357 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVRP010000318 DAJVRP010000000
VERSION     DAJVRP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.327"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
BASE COUNT          106 a           97 c           66 g           88 t
ORIGIN      
        1 cccacccaaa ccaataagga ctaacatgac ttttaactgt gccgcgtgcg catagccgca
       61 ttcaccgcat cacaaaattc actttaaaaa gggcggacat cagccagcaa ttaaaccgat
      121 gccgccaact ggtacttcac acagcaatgt cgttatttac aaccggaagc gcactcccac
      181 catttaaatt tcacagacaa gaccgactct ttatggatac cggaaatgcg ccttcgtgtt
      241 gtgcccggtt ttatttcacc acttccgggc tttggtggta tctttactga agttctcaca
      301 caaccagtaa ggaaatgaat atgccaacgt atctcgccag agtagaacta tataatg
//
LOCUS       DAJVRP010000319          355 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVRP010000319 DAJVRP010000000
VERSION     DAJVRP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.328"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            1..336
                     /locus_tag="OQN12_004256"
                     /pseudo
     CDS             1..336
                     /locus_tag="OQN12_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          128 a           75 c           82 g           70 t
ORIGIN      
        1 tgaattattt gccaacgacg gtacaccgac atggctaaaa gaaaagcgtg caaaagaaat
       61 cgcacttgat gaaattaact tgatgaaatt atcgagttgc tcaacaagca ccatgggggc
      121 accagtaacg acgccaaacg cgcaaaagct gatttgctgg aaaaaacatt cacctcccgc
      181 tcatgggaaa gaatcaaagg tatggactgg ccgacaatca aagctggtcg taatgcacta
      241 tggattgaac tggaaggcgt tgaatatgca ttcccggatc cacaaacaca aaacgaaacg
      301 caacaagcag gatacgatga gaatattccg gtgtaaaacc aagtgcattt tggga
//
LOCUS       DAJVRP010000320          352 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVRP010000320 DAJVRP010000000
VERSION     DAJVRP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.329"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
BASE COUNT          103 a           76 c           67 g          106 t
ORIGIN      
        1 ataactgtgg cttaacacct gtacaagcga aaaaaatcaa tcacctgtcc ggaaatacgg
       61 gatcactgta aagtgttggt gaggcagtct aggaggggga tttaatttgg gatactgatg
      121 aaaggattat gatcagttgg atataggatc gtactaagct gagcggcagg ttagctagat
      181 tagttaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat
      241 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
      301 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac ag
//
LOCUS       DAJVRP010000321          351 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVRP010000321 DAJVRP010000000
VERSION     DAJVRP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.330"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..239)
                     /locus_tag="OQN12_004257"
     CDS             complement(<1..239)
                     /locus_tag="OQN12_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCS1656359.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT           95 a           64 c           83 g          109 t
ORIGIN      
        1 taacataaaa acgcaaaagc ccaaaagtca gagcttaggt gttctaggcg ttttctaagc
       61 gctgaagaaa tacgtcttac cgtagattgc gtgcgtttct gaagcacatt ttccttatac
      121 atggctttat cccaggcagc ttcatcaagc tcttggagta acaagccagt aataatgcga
      181 cactctcggg tttgtagcga gccgctggtg agatcggagt tatagcggaa aggggtcatt
      241 tgtaattatt tgtgggtgga ggatagttaa cttattattg tagttggaag aggctgcttt
      301 ccaaacatta gcacctcgta atgcttgtta ctgtatatta atttatgctc t
//
LOCUS       DAJVRP010000322          346 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVRP010000322 DAJVRP010000000
VERSION     DAJVRP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.331"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>346
                     /locus_tag="OQN12_004258"
     CDS             <1..>346
                     /locus_tag="OQN12_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483257.1"
                     /note="serine sensor receptor; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCS1656360.1"
                     /translation="TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD
                     IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLA
                     NTLTIDGRSEMG"
BASE COUNT           83 a           78 c           94 g           91 t
ORIGIN      
        1 taactgaact gattgattat cttgattatg gcaatactgg agcttatttc gctcagccaa
       61 cccagggaat gcaaaatgca atgggcgaag cgtttgctca gtacgccctc agcagtgaaa
      121 aactgtatcg cgatatcgtc actgacaacg cagatgatta ccgatttgcc cagtggcaac
      181 tggcggttat cgcgctggtg gtggtattga ttctgctggt ggcgtggtac ggcattcgcc
      241 gtatgttgct tactccgctg gcaaaaatta ttgctcacat ttgcgaaatc gccggtggta
      301 acctggcgaa taccctgacc attgacgggc gcagtgaaat gggtga
//
LOCUS       DAJVRP010000323          344 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVRP010000323 DAJVRP010000000
VERSION     DAJVRP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.332"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OQN12_004259"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OQN12_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1656361.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJVRP010000324          340 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVRP010000324 DAJVRP010000000
VERSION     DAJVRP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.333"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..>340)
                     /locus_tag="OQN12_004260"
     CDS             complement(<1..>340)
                     /locus_tag="OQN12_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1656362.1"
                     /translation="KSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT           76 a           97 c           88 g           79 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc
//
LOCUS       DAJVRP010000325          338 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVRP010000325 DAJVRP010000000
VERSION     DAJVRP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.334"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>338
                     /locus_tag="OQN12_004261"
     CDS             <1..>338
                     /locus_tag="OQN12_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1656363.1"
                     /translation="NFKQKNEALDTWYVGMNDFARIAGGQNSRSNILSPRAFLEFLAK
                     IFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKFYMNNPEGERADLSKVKI
                     EITLASASTK"
BASE COUNT          111 a           52 c           76 g           99 t
ORIGIN      
        1 acaattttaa acaaaaaaat gaagccttag atacctggta tgtgggaatg aatgattttg
       61 cccgaattgc cggggggcag aatagcagaa gcaatattct ttctcctaga gcatttttgg
      121 agtttttggc aaagatattt accctgggtt atgtggattt tagcaaacgc tccaacgaag
      181 cgggtagaaa tatgatggct catattgagt tctcatctta ttctaaagat actgatggca
      241 atgaaaaaat gaagttttac atgaataatc ctgaagggga gcgagcggat ttatctaagg
      301 tgaagataga aattacactt gcctcagctt ctactaaa
//
LOCUS       DAJVRP010000326          323 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVRP010000326 DAJVRP010000000
VERSION     DAJVRP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.335"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
BASE COUNT          107 a           79 c           51 g           86 t
ORIGIN      
        1 agaaatattt gcaaaaatag tgcatgaaaa aagcttttcc ctaccaatcc gggatataaa
       61 taatcatcag taccacaatt attccacagc aataaatctc ttcccgccgc tccggacctc
      121 tgataatggt ctaaaatcat tgaggccact tgcgacgaca ttgctcaatg aggaaagttt
      181 ggcgtgaata atattcaaca ccagtgcagc atcatcatta catacttatg cccgccaact
      241 accgctttcg atatattgac tcaatttttc agcctggtta aaaatagcga ccagctcagt
      301 cgcaaatata gtgactaccc taa
//
LOCUS       DAJVRP010000327          312 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVRP010000327 DAJVRP010000000
VERSION     DAJVRP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.336"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
BASE COUNT           78 a           64 c           79 g           91 t
ORIGIN      
        1 tgttagtctt tgcatgagca agcggactga cacatttctc tttgttctca ttcagaaaat
       61 ctcatcagtc ggcgttcaga ccggtgttgt gctttatgga tacgttacgc tcctgatgac
      121 gtcatttgac gttcaacagc atcacggggc cgcacgacat ttcacgtcag ttagtgctat
      181 agctcaggaa caaattttcc caaattggga tatgctcgca aattgctggt gatgtgggga
      241 gaatctggtt gagttcggta gaattgattt ggaggcagaa cgcttaaatc gtggcgtcct
      301 gaaacgaaaa ac
//
LOCUS       DAJVRP010000328          303 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVRP010000328 DAJVRP010000000
VERSION     DAJVRP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.337"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            69..>303
                     /locus_tag="OQN12_004262"
     CDS             69..>303
                     /locus_tag="OQN12_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1656364.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDD"
BASE COUNT           98 a           61 c           84 g           60 t
ORIGIN      
        1 gcggctcttc agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag
       61 gaaagaaaat gtcactcttc cgcagaggtg aaatatggta cgccagtttc acattgccga
      121 acggtaaaag atttaaacag tctcttggaa caaaggacaa aaggcaggcg acagaactcc
      181 atgacaagct aaaggctgaa gcatggcggg tcagcaaact tggtgaaata cctgatataa
      241 cgttcgagga agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa tcactggacg
      301 atg
//
LOCUS       DAJVRP010000329          302 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVRP010000329 DAJVRP010000000
VERSION     DAJVRP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.338"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
BASE COUNT           86 a           73 c           59 g           84 t
ORIGIN      
        1 ccccccgaca gacctgtgct atcggaaaga aaaaaggcca tcagcaaaac tctgatgacc
       61 tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg actaattgac aataattttc
      121 atttccatta aaaaagatcg cgtagcattc ttaattcatg aggaacttta cccgccagca
      181 atctgagtag cgaaagctgt tcatccccaa tgtttttgct ggcgggtcct tttttctacg
      241 gtcctcctcc ccggaggggg gcatttcatt atttttctaa tcgttactgg tgagaaggcg
      301 ga
//
LOCUS       DAJVRP010000330          299 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVRP010000330 DAJVRP010000000
VERSION     DAJVRP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.339"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            <1..>299
                     /locus_tag="OQN12_004263"
     CDS             <1..>299
                     /locus_tag="OQN12_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1656365.1"
                     /translation="NPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPL
                     GVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGEN"
BASE COUNT           86 a           67 c           70 g           76 t
ORIGIN      
        1 aaatccgcgt aattttacgt ggaatattat ttcaaataac agtgttgtta tgccaacagg
       61 cggctgcacc gttgatagtc gtaatgtcac cgtggatctc ccggactttc ctggaagcgc
      121 agaaataccc cttggcgtat attgctcaag cgaacaaaag ctctcatttt atctttcggg
      181 tgccaccact gacagtagcc gacaagtgtt tgctaacaca gcacctgatg caacaaaagc
      241 atcgggagtg ggtgtcactc taatgcgtaa tggaaaaata ctggctacgg gagaaaatg
//
LOCUS       DAJVRP010000331          295 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVRP010000331 DAJVRP010000000
VERSION     DAJVRP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.340"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..>295)
                     /locus_tag="OQN12_004264"
     CDS             complement(<1..>295)
                     /locus_tag="OQN12_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656366.1"
                     /translation="VDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTA
                     LAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQETVNKA"
BASE COUNT           59 a           89 c           79 g           68 t
ORIGIN      
        1 cagccttgtt taccgtttcc tgtaaaccga ggtattcgat aacggcggca acggtcgatt
       61 tcgcaagaat atcccgcccg acttttgtca gggttgccag gctggcgaca tcattccccg
      121 taaaatacgg aaacctgtct gccgcagtag caagccccgc cagcgccgtc agagtggcat
      181 ctttcggttg cttacccgca agcgcgttag tcatggtggt cgcaaaattc gggtcgttgc
      241 ccagcgccgc cgccagttca ttcagcgtgt tcagtgcatc aggcgacgag tccac
//
LOCUS       DAJVRP010000332          295 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVRP010000332 DAJVRP010000000
VERSION     DAJVRP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.denovo.341"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(23..>295)
                     /locus_tag="OQN12_004265"
     CDS             complement(23..>295)
                     /locus_tag="OQN12_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1656367.1"
                     /translation="LGVYLGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGN
                     IAGNAAIIHFAGWIGLICGASAIYLAMGEVLNEQFGRTVLPIGESH"
BASE COUNT           89 a           84 c           79 g           43 t
ORIGIN      
        1 gagcgggggg atctcaagac aattagtggg attcaccaat cggcagaacg gtgcgaccaa
       61 actgctcgtt cagcacttca cccatcgcca gatagattgc gctggcaccg cagatcagac
      121 caatccagcc ggcaaagtgg atgatcgcgg cattaccggc aatgttaccg atcgccagca
      181 gggcaaacag cacggtcagg ctaaagaaaa cgaattgcag gacgcgcgcg cctttcagag
      241 tgccgaagaa cataaacagc gtaaatacgc cccacagacc caggtagaca ccaag
//
LOCUS       DAJVRP010000333         5962 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.guided.168, whole genome shotgun sequence.
ACCESSION   DAJVRP010000333 DAJVRP010000000
VERSION     DAJVRP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.guided.168"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OQN12_004266"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OQN12_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1656368.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OQN12_004267"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OQN12_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCS1656369.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3235
                     /locus_tag="OQN12_004268"
     CDS             2399..3235
                     /locus_tag="OQN12_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1656370.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3342..3746)
                     /locus_tag="OQN12_004269"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OQN12_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OQN12_004270"
     CDS             complement(3790..4179)
                     /locus_tag="OQN12_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656371.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OQN12_004271"
     CDS             4397..4837
                     /locus_tag="OQN12_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1656372.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5582..5731)
                     /locus_tag="OQN12_004272"
     CDS             complement(5582..5731)
                     /locus_tag="OQN12_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1656373.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1451 a         1571 c         1553 g         1387 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggtttgttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5281 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5341 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     5401 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     5461 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     5521 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     5581 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt
     5641 cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg
     5701 aatccagttg ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt
     5761 tagcgggttt cggggcgcag ccctgaacca gtcatgtagc gctagcggag tgtatactgg
     5821 cttactatgt tgccacgatt gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa
     5881 ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga tattccgctt cctcgctcac
     5941 tgactcgcta cgctcggtcg tt
//
LOCUS       DAJVRP010000334         5055 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.guided.183, whole genome shotgun sequence.
ACCESSION   DAJVRP010000334 DAJVRP010000000
VERSION     DAJVRP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.guided.183"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            18..1826
                     /gene="frdA"
                     /locus_tag="OQN12_004273"
     CDS             18..1826
                     /gene="frdA"
                     /locus_tag="OQN12_004273"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1656374.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            1819..2553
                     /gene="frdB"
                     /locus_tag="OQN12_004274"
     CDS             1819..2553
                     /gene="frdB"
                     /locus_tag="OQN12_004274"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1656375.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2564..2959
                     /gene="frdC"
                     /locus_tag="OQN12_004275"
     CDS             2564..2959
                     /gene="frdC"
                     /locus_tag="OQN12_004275"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1656376.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2970..3329
                     /gene="frdD"
                     /locus_tag="OQN12_004276"
     CDS             2970..3329
                     /gene="frdD"
                     /locus_tag="OQN12_004276"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1656377.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            3392..4525
                     /locus_tag="OQN12_004277"
     CDS             3392..4525
                     /locus_tag="OQN12_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1656378.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4614..>5055
                     /gene="blc"
                     /locus_tag="OQN12_004278"
     CDS             4614..>5055
                     /gene="blc"
                     /locus_tag="OQN12_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1656379.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTI"
BASE COUNT         1214 a         1330 c         1362 g         1149 t
ORIGIN      
        1 caatctggag gaatgtcgtg caaacctttc aagccgatct tgccattgta ggcgccggtg
       61 gcgcgggatt acgtgctgca attgctgccg cgcaggcaaa tccaaatgca aaaatcgcac
      121 taatctcaaa agtatacccg atgcgtagcc ataccgttgc tgcagaaggg ggctccgccg
      181 ctgtcgcgca ggatcatgac agcttcgaat atcactttca cgatacagta gcgggtggcg
      241 actggttgtg tgagcaggat gtcgtggatt atttcgtcca ccactgccca accgaaatga
      301 cccaactgga actgtgggga tgcccatgga gccgtcgccc ggatggtagc gtcaacgtac
      361 gtcgcttcgg cggcatgaaa atcgagcgca cctggttcgc cgccgataag accggcttcc
      421 atatgctgca cacgctgttc cagacctctc tgcaattccc gcagatccag cgttttgacg
      481 aacatttcgt gctggatatt ctggttgatg atggtcatgt tcgcggcctg gtagcaataa
      541 acatgatgga aggcacgttg gtgcagatcc gtgctaacgc ggtcgttatg gctaccggcg
      601 gtgcgggtcg cgtttatcgt tacaacacca acggcggcat cgttaccggt gacggtatgg
      661 gtatggcgct aagccacggc gttccgctgc gtgacatgga attcgttcag tatcacccaa
      721 ccggtctgcc aggttccggt atcctgatga ccgaaggctg ccgtggtgaa ggcggtattc
      781 tggtcaacaa aaatggctac cgttatctgc aagattacgg catgggcccg gaaactccgc
      841 tgggcgagcc gaaaaacaaa tatatggaac tgggtccacg cgacaaagtt tctcaggcct
      901 tctggcacga atggcgtaaa ggcaacacca tccccacgcc gcgtggtgat gtggtttatc
      961 tcgacctgcg tcacctcggc gagaaaaaac tgcatgaacg tctgccgttc atctgcgaac
     1021 tggcgaaagc gtacgttggc gtcgatccgg ttaaagaacc gattccggta cgtccgactg
     1081 cacactacac catgggcggt atcgaaaccg atcagaactg tgaaacccgc attaaaggtc
     1141 tgttcgccgt gggtgaatgt tcctctgttg gtctgcacgg tgcaaaccgt ctgggctcca
     1201 actccctggc ggaactggtg gtcttcggcc gtctggccgg tgaacaagcg acagagcgtg
     1261 cagcaactgc cggtaatggc aacgaagcgg caattgaagc gcaggcagct ggcgttgaac
     1321 aacgtctgaa agatctagtt aaccaggatg gcggcgaaaa ctgggcgaag atccgcgacg
     1381 aaatgggcct ggcaatggaa gaaggttgcg gtatctaccg tacgccggaa ctgatgcaga
     1441 aaaccatcga caagctggca gagctgcagg aacgcttcaa gcgcgtgcgc atcaccgaca
     1501 cctccagcgt gttcaacacc gacctgctct acaccattga actgagccac ggtctgaacg
     1561 ttgctgaatg tatggcgcac tccgcaatgg cacgtaaaga gtcccgcggc gcacaccagc
     1621 gtctggacga aggttgcacc gagcgtgacg acgtcaactt tctcaaacac accctcgcct
     1681 tccgcgatgc tgatggcacg actcgcctgg agtacagcga cgtgaagatt actacgctgc
     1741 cgccagctaa acgcgtttac ggtggcgaag cggatgcagc cgataaggcg gaagcagcca
     1801 ataataagga gaaggcgaat ggctgagatg aaaaacctga aaattgaggt ggtgcgctat
     1861 aacccggaag tcgataccgc accgcatagc gcattctatg aagtgcctta tgacgcaact
     1921 acctcattac tggatgcgct gggctacatc aaagacaacc tggcaccgga cctgagttac
     1981 cgctggtcct gccgtatggc gatttgtggt tcctgcggca tgatggttaa caacgtgcca
     2041 aaactggcat gtaaaacctt cctgcgtgat tacaccgacg gtatgaaggt tgaagcgtta
     2101 gctaacttcc cgattgaacg cgatctggtg gtcgatatga cccacttcat cgaaagtctg
     2161 gaagcgatca aaccgtacat catcggcaac tcccgcaccg cggatcaggg tactaacatc
     2221 cagaccccgg cgcagatggc aaagtatcac cagttctccg gttgcatcaa ctgtggtctg
     2281 tgctacgccg cgtgcccgca gtttggcctg aacccagagt tcatcggtcc ggctgccatt
     2341 acgctggcgc atcgttataa cgaagatagc cgcgaccacg gtaagaagga gcgtatggcg
     2401 cagttgaaca gccagaacgg tgtatggagc tgtactttcg tgggctattg ctccgaagtc
     2461 tgcccgaaac acgtcgatcc ggctgcggcc attcagcagg gcaaagtaga aagttcgaaa
     2521 gactttctta tcgcgaccct gaaaccacgc taaggagtgc aacatgacga ctaaacgtaa
     2581 accgtatgta cggccaatga cgtccacctg gtggaaaaaa ttgccgtttt atcgctttta
     2641 catgctgcgc gaaggcacgg cggttccggc tgtgtggttc agcattgaac tgattttcgg
     2701 gctgtttgcc ctgaaaaatg gcccggaagc ctgggcggga ttcgtcgact ttttacaaaa
     2761 cccggttatc gtgatcatta acctgatcac tctgacggca gctctgctgc acaccaaaac
     2821 ctggtttgaa ctggcaccga aagcggccaa tatcattgta aaagacgaaa aaatgggacc
     2881 agagccaatt atcaaaagtc tctgggcggt aactgtggtt gccaccatcg taatcctgtt
     2941 tgttgccctg tactggtaag gagcctgaga tgattaatcc aaatccaaag cgttctgacg
     3001 aaccggtatt ctggggcctc ttcggggccg gtggtatgtg gagcgcaatt attgcgccgg
     3061 tgatgatcct gctggtgggt attctgctgc cactggggct gtttccgggt gatgcgctga
     3121 gctacgagcg cgttctggcg ttcgcgcaga gcttcattgg tcgcgtattc ctgttcctga
     3181 tgatcgttct gccgctgtgg tgtggtttac accgtatgca ccacgcgatg cacgatctga
     3241 aaatccacgt gcctgcgggc aaatgggttt tctacggtct ggctgctatc ctgacagttg
     3301 tcacgctgat tggtgtcgtt acaatctaac gcatcgccaa tgtaaatccg gcccgcctat
     3361 ggcgggccgt tttgtatgga aaccagacct tatgttcaaa acgacgctct gcgccttatt
     3421 aattaccgcc tcttactcca catttgctgc ccctcaacaa atcaacgata ttgtgcatcg
     3481 cacaattacc ccgcttatag agcaacaaaa gatccccggt atggcggtgg cggtaattta
     3541 tcagggtaaa ccttattact ttacctgggg ctatgcggac atcgccaaaa agcagcccgt
     3601 cacacagcaa acgttgtttg agttaggttc ggtcagcaaa acatttacgg gcgtgcttgg
     3661 tggcgacgct attgctcgag gggaaatcaa gttaagcgat cccgcaacaa aatactggcc
     3721 tgaacttacc gctaaacagt ggaatgggat cacactatta catcttgcga cctacaccgc
     3781 tggcggcctg ccattgcagg tgccggatga agtgaaatcc tcaagcgact tgctgcgctt
     3841 ctatcaaaac tggcagcctg catgggctcc aggaacacaa cgtctgtatg ccaactccag
     3901 tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt ttgagttttg agcaggcgat
     3961 gcaaactcgt gtcttccagc cactcaaact caaccatacg tggattaatg tacctccccc
     4021 agaagaaaag aattacgcct ggggatatcg cgaaggtaag gcagtgcatg tttcgccagg
     4081 ggcgttagat gctgaagctt atggtgtgaa gtcgaccatt gaagatatgg cccgctgggt
     4141 gcaaagcaat ttaccccccc ttgatatcaa tgagaaaacg cttcaacaag ggatacaact
     4201 ggcacaatct cgctactggc aaaccggcga tatgtatcag ggcctgggct gggaaatgct
     4261 ggactggccg gtcaatcctg acagcatcat taacggcagt gacaataaaa ttgcactggc
     4321 agcacgcccg gtaaaagcga ttaagccccc aactcctgca gtacgcgcat catgggtaca
     4381 taaaacgggg gcgaccggcg gatttggtag ctatgtcgcg tttattccag aaaaagagct
     4441 gggtatcgtg atgctggcta acaaaaacta tcccaatcca gcgagagtcg ccgccgcctg
     4501 tcagattctc aacgctctac agtaaaattt catcgggtcc gaattttcgg accttttctg
     4561 cgcttttcct tgctgtcatc tacacttaga aaaaaaccag taaggaaaca tttatgcgcc
     4621 tgctccctct cgttgccgca gcgacagctg catttctggt cgttgcctgt agttttccta
     4681 cgccgccgcg tggcgtgacc gtagtgaata atttcgacgc caaacgttat ctcggtacct
     4741 ggtatgagat tgcccgtttt gatcaccgtt ttgaacgtgg actggaaaaa gtcaccgcaa
     4801 catacagcct gcgtgatgac ggcggcctga atgtcattaa taaaggctat aaccctgaca
     4861 gaggaatgtg gcagcagagt gaagggaaag cgtactttac cggcgcacca actcgcgctg
     4921 cgctgaaagt gtcattcttt ggtcctttct atggcggtta taacgttatt gcactcgatc
     4981 gggaataccg ccatgcgctg gtttgcggcc cggaccgcga ctacctgtgg atactctccc
     5041 gcacgccaac cattt
//
LOCUS       DAJVRP010000335         3892 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.guided.202, whole genome shotgun sequence.
ACCESSION   DAJVRP010000335 DAJVRP010000000
VERSION     DAJVRP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.guided.202"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..196)
                     /locus_tag="OQN12_004279"
     CDS             complement(<1..196)
                     /locus_tag="OQN12_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1656380.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDR"
     gene            complement(322..672)
                     /locus_tag="OQN12_004280"
     CDS             complement(322..672)
                     /locus_tag="OQN12_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCS1656381.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(875..1888)
                     /gene="intI1"
                     /locus_tag="OQN12_004281"
     CDS             complement(875..1888)
                     /gene="intI1"
                     /locus_tag="OQN12_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1656382.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2098..2928
                     /locus_tag="OQN12_004282"
     CDS             2098..2928
                     /locus_tag="OQN12_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCS1656383.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            3041..3832
                     /gene="aadA1"
                     /locus_tag="OQN12_004283"
     CDS             3041..3832
                     /gene="aadA1"
                     /locus_tag="OQN12_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1656384.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1011 a         1024 c         1007 g          850 t
ORIGIN      
        1 ggcgatccat gctatgcacc accacggtgt cgccggtgcg ggcgaagctt atcagcgctt
       61 ccagttgcgg acgcttgaca tccttgccgg atgccttgtc gctaaaagcg cgatcaacct
      121 tgacgccttc cagttgccgt tccgggttct ggtcgaaggt gctgaccctg atatacccaa
      181 tgcgctgtcc agtcatggaa ttccctgcaa aatgtcaggg aagactctat gaccttcaac
      241 gagatatgtc aataaattca aaattcaatc ctatcctgac gcaatttaca catggcatct
      301 gacatcaggt tagggtatgc ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac
      361 gctggtttcg gtcgcagtgc tggcccgcgc ctggaagcgc tgatagcact ccagcccgca
      421 gaaatgctcc acgtactcgg ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt
      481 gcagcacacg cagcaactgg tgctcggttc attggcgttc atggtggtgt tcctccattg
      541 gttgacgaag ccgacgaagg ccgccgccgg catcggcctg gcgaacagga aaccctgcac
      601 cgtgtcgcaa cccgcctgcc gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc
      661 taccacctcc attgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
      721 tatagcagcg gaggggttgg atccatcagg caacgacggg ctgctgccgg ccatcagcgg
      781 acgcagggag gactttccgc aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg
      841 ggtagtgaat ccgccaggat tgacttgcgc tgccctacct ctcactagtg aggggcggca
      901 gcgcatcaag cggtgagcgc actccggcac cgccaacttt cagcacatgc gtgtaaatca
      961 tcgtcgtaga gacgtcggaa tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc
     1021 tgcggagcaa ggccgtcgcg aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga
     1081 tgcctgcttg ttctacggca cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat
     1141 ggcgacgcac gacaccgctc cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga
     1201 accacggcca ggaatgcccg gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa
     1261 cgccgctgcg gccctcggcc tggtccttca gccaccatgc ccgtgcacgc gacagctgct
     1321 cgcgcaggct gggtgccaag ctctcgggta acatcaaggc ccgatccttg gagcccttgc
     1381 cctcccgcac gatgatcgtg ccgtgatcga aatccagatc cttgacccgc agttgcaaac
     1441 cctcactgat ccgcatgccc gttccataca gaagctgggc gaacaaacga tgctcgcctt
     1501 ccagaaaacc gaggatgcga accacttcat ccggggtcag caccaccggc aagcgccgcg
     1561 acggccgagg tcttccgatc tcctgaagcc agggcagatc cgtgcacagc accttgccgt
     1621 agaagaacag caaggccgcc aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt
     1681 tcgccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca
     1741 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     1801 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1861 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     1921 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     1981 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagttg ggcgaacccg
     2041 gagcctcatt aattgttagc cgttaaaatt aagcccttta ccaaaccaat acttattatg
     2101 aaaaacacaa tacatatcaa cttcgctatt tttttaataa ttgcaaatat tatctacagc
     2161 agcgccagtg catcaacaga tatctctact gttgcatctc cattatttga aggaactgaa
     2221 ggttgttttt tactttacga tgcatccaca aacgctgaaa ttgctcaatt caataaagca
     2281 aagtgtgcaa cgcaaatggc accagattca actttcaaga tcgcattatc acttatggca
     2341 tttgatgcgg aaataataga tcagaaaacc atattcaaat gggataaaac ccccaaagga
     2401 atggagatct ggaacagcaa tcatacacca aagacgtgga tgcaattttc tgttgtttgg
     2461 gtttcgcaag aaataaccca aaaaattgga ttaaataaaa tcaagaatta tctcaaagat
     2521 tttgattatg gaaatcaaga cttctctgga gataaagaaa gaaacaacgg attaacagaa
     2581 gcatggctcg aaagtagctt aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa
     2641 attattaatc acaatctccc agttaaaaac tcagccatag aaaacaccat agagaacatg
     2701 tatctacaag atctggataa tagtacaaaa ctgtatggga aaactggtgc aggattcaca
     2761 gcaaatagaa ccttacaaaa cggatggttt gaagggttta ttataagcaa atcaggacat
     2821 aaatatgttt ttgtgtccgc acttacagga aacttggggt cgaatttaac atcaagcata
     2881 aaagccaaga aaaatgcgat caccattcta aacacactaa atttataaaa aatctaatgg
     2941 caaaatcgcc caacccttca atcaagtcgg gacggccaaa agcaagcttt tggctcccct
     3001 cgctggcgct cggcgcccct tatttcaaac gttaaacatc atgagggaag tggtgatcgc
     3061 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     3121 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     3181 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     3241 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     3301 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     3361 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     3421 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     3481 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     3541 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     3601 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     3661 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     3721 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     3781 agaatttgtt cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
     3841 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAJVRP010000336         3889 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.guided.203, whole genome shotgun sequence.
ACCESSION   DAJVRP010000336 DAJVRP010000000
VERSION     DAJVRP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.guided.203"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..196)
                     /locus_tag="OQN12_004284"
     CDS             complement(<1..196)
                     /locus_tag="OQN12_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1656385.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDR"
     gene            complement(322..672)
                     /locus_tag="OQN12_004285"
     CDS             complement(322..672)
                     /locus_tag="OQN12_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCS1656386.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(875..1888)
                     /gene="intI1"
                     /locus_tag="OQN12_004286"
     CDS             complement(875..1888)
                     /gene="intI1"
                     /locus_tag="OQN12_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1656387.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2098..2928
                     /locus_tag="OQN12_004287"
     CDS             2098..2928
                     /locus_tag="OQN12_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCS1656388.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            3041..3829
                     /gene="aadA1"
                     /locus_tag="OQN12_004288"
     CDS             3041..3829
                     /gene="aadA1"
                     /locus_tag="OQN12_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1656389.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1008 a         1024 c         1007 g          850 t
ORIGIN      
        1 ggcgatccat gctatgcacc accacggtgt cgccggtgcg ggcgaagctt atcagcgctt
       61 ccagttgcgg acgcttgaca tccttgccgg atgccttgtc gctaaaagcg cgatcaacct
      121 tgacgccttc cagttgccgt tccgggttct ggtcgaaggt gctgaccctg atatacccaa
      181 tgcgctgtcc agtcatggaa ttccctgcaa aatgtcaggg aagactctat gaccttcaac
      241 gagatatgtc aataaattca aaattcaatc ctatcctgac gcaatttaca catggcatct
      301 gacatcaggt tagggtatgc ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac
      361 gctggtttcg gtcgcagtgc tggcccgcgc ctggaagcgc tgatagcact ccagcccgca
      421 gaaatgctcc acgtactcgg ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt
      481 gcagcacacg cagcaactgg tgctcggttc attggcgttc atggtggtgt tcctccattg
      541 gttgacgaag ccgacgaagg ccgccgccgg catcggcctg gcgaacagga aaccctgcac
      601 cgtgtcgcaa cccgcctgcc gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc
      661 taccacctcc attgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
      721 tatagcagcg gaggggttgg atccatcagg caacgacggg ctgctgccgg ccatcagcgg
      781 acgcagggag gactttccgc aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg
      841 ggtagtgaat ccgccaggat tgacttgcgc tgccctacct ctcactagtg aggggcggca
      901 gcgcatcaag cggtgagcgc actccggcac cgccaacttt cagcacatgc gtgtaaatca
      961 tcgtcgtaga gacgtcggaa tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc
     1021 tgcggagcaa ggccgtcgcg aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga
     1081 tgcctgcttg ttctacggca cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat
     1141 ggcgacgcac gacaccgctc cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga
     1201 accacggcca ggaatgcccg gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa
     1261 cgccgctgcg gccctcggcc tggtccttca gccaccatgc ccgtgcacgc gacagctgct
     1321 cgcgcaggct gggtgccaag ctctcgggta acatcaaggc ccgatccttg gagcccttgc
     1381 cctcccgcac gatgatcgtg ccgtgatcga aatccagatc cttgacccgc agttgcaaac
     1441 cctcactgat ccgcatgccc gttccataca gaagctgggc gaacaaacga tgctcgcctt
     1501 ccagaaaacc gaggatgcga accacttcat ccggggtcag caccaccggc aagcgccgcg
     1561 acggccgagg tcttccgatc tcctgaagcc agggcagatc cgtgcacagc accttgccgt
     1621 agaagaacag caaggccgcc aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt
     1681 tcgccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca
     1741 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     1801 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1861 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     1921 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     1981 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagttg ggcgaacccg
     2041 gagcctcatt aattgttagc cgttaaaatt aagcccttta ccaaaccaat acttattatg
     2101 aaaaacacaa tacatatcaa cttcgctatt tttttaataa ttgcaaatat tatctacagc
     2161 agcgccagtg catcaacaga tatctctact gttgcatctc cattatttga aggaactgaa
     2221 ggttgttttt tactttacga tgcatccaca aacgctgaaa ttgctcaatt caataaagca
     2281 aagtgtgcaa cgcaaatggc accagattca actttcaaga tcgcattatc acttatggca
     2341 tttgatgcgg aaataataga tcagaaaacc atattcaaat gggataaaac ccccaaagga
     2401 atggagatct ggaacagcaa tcatacacca aagacgtgga tgcaattttc tgttgtttgg
     2461 gtttcgcaag aaataaccca aaaaattgga ttaaataaaa tcaagaatta tctcaaagat
     2521 tttgattatg gaaatcaaga cttctctgga gataaagaaa gaaacaacgg attaacagaa
     2581 gcatggctcg aaagtagctt aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa
     2641 attattaatc acaatctccc agttaaaaac tcagccatag aaaacaccat agagaacatg
     2701 tatctacaag atctggataa tagtacaaaa ctgtatggga aaactggtgc aggattcaca
     2761 gcaaatagaa ccttacaaaa cggatggttt gaagggttta ttataagcaa atcaggacat
     2821 aaatatgttt ttgtgtccgc acttacagga aacttggggt cgaatttaac atcaagcata
     2881 aaagccaaga aaaatgcgat caccattcta aacacactaa atttataaaa aatctaatgg
     2941 caaaatcgcc caacccttca atcaagtcgg gacggccaaa agcaagcttt tggctcccct
     3001 cgctggcgct cggcgcccct tatttcaaac gttaaacatc atgagggaag tggtgatcgc
     3061 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     3121 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     3181 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     3241 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     3301 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     3361 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     3421 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     3481 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     3541 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     3601 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     3661 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     3721 taggcaggct tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     3781 atttgttcac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat
     3841 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttaga
//
LOCUS       DAJVRP010000337         3692 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.guided.206, whole genome shotgun sequence.
ACCESSION   DAJVRP010000337 DAJVRP010000000
VERSION     DAJVRP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.guided.206"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            68..1273
                     /gene="tet(B)"
                     /locus_tag="OQN12_004289"
     CDS             68..1273
                     /gene="tet(B)"
                     /locus_tag="OQN12_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1656390.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1386..1979)
                     /gene="tetC"
                     /locus_tag="OQN12_004290"
     CDS             complement(1386..1979)
                     /gene="tetC"
                     /locus_tag="OQN12_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCS1656391.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2112..2483
                     /locus_tag="OQN12_004291"
     CDS             2112..2483
                     /locus_tag="OQN12_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1656392.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(2493..>3692)
                     /locus_tag="OQN12_004292"
     CDS             complement(2493..>3692)
                     /locus_tag="OQN12_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCS1656393.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT          976 a          681 c          812 g         1223 t
ORIGIN      
        1 ttttgttgac actctatcat tgatagagtt attttaccac tccctatcag tgatagagaa
       61 aagtgaaatg aatagttcga caaagatcgc attggtaatt acgttactcg atgccatggg
      121 gattggcctt atcatgccag tcttgccaac gttattacgt gaatttattg cttcggaaga
      181 tatcgctaac cactttggcg tattgcttgc actttatgcg ttaatgcagg ttatctttgc
      241 tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt
      301 aataggcgca tcgctggatt acttattgct ggctttttca agtgcgcttt ggatgctgta
      361 tttaggccgt ttgctttcag ggatcacagg agctactggg gctgtcgcgg catcggtcat
      421 tgccgatacc acctcagctt ctcaacgcgt gaagtggttc ggttggttag gggcaagttt
      481 tgggcttggt ttaatagcgg ggcctattat tggtggtttt gcaggagaga tttcaccgca
      541 tagtcccttt tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg ttatgttttg
      601 gttccgtgaa accaaaaata cacgtgataa tacagatacc gaagtagggg ttgagacgca
      661 atcgaattcg gtatacatca ctttatttaa aacgatgccc attttgttga ttatttattt
      721 ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg
      781 ttttggatgg aatagcatga tggttggctt ttcattagcg ggtcttggtc ttttacactc
      841 agtattccaa gcctttgtgg caggaagaat agccactaaa tggggcgaaa aaacggcagt
      901 actgctcgga tttattgcag atagtagtgc atttgccttt ttagcgttta tatctgaagg
      961 ttggttagtt ttccctgttt taattttatt ggctggtggt gggatcgctt tacctgcatt
     1021 acagggagtg atgtctatcc aaacaaagag tcatcagcaa ggtgctttac agggattatt
     1081 ggtgagcctt accaatgcaa ccggtgttat tggcccatta ctgtttgctg ttatttataa
     1141 tcattcacta ccaatttggg atggctggat ttggattatt ggtttagcgt tttactgtat
     1201 tattatcctg ctatcgatga ccttcatgtt aacccctcaa gctcagggga gtaaacagga
     1261 gacaagtgct tagttatttc gtcaccaaat gatgttattc cgcgaaatat aatgaccctc
     1321 ttgataaccc aagagggcat tttttacgat aaagaagatt tagcttcaaa taaaacctat
     1381 ctattttatt tatctttcaa gctcaataaa aagccgcggt aaatagcaat aaattggcct
     1441 tttttatcgg caagctcttt taggtttttc gcatgtattg cgatatgcat aaaccagcca
     1501 ttgagtaagt ttttaagcac atcatcatca taagctttaa gttggttctc ttggatcaat
     1561 ttgctgacaa tggcgtttac cttaccagta atgtattcaa ggctaatttt ttcaagttca
     1621 ttccaaccaa tgataggcat cacttcttgg atagggataa ggtttttatt attatcaata
     1681 atataatcaa gataatgttc aaatatactt tctaaggcag accaaccatt tgttaaatca
     1741 gtttttgttg tgatgtaggc atcaatcata attaattgct gcttataaca ggcactgagt
     1801 aattgttttt tatttttaaa gtgatgataa aaggcacctt tggtcaccaa cgcttttccc
     1861 gagatctcat ctattgaaac agcttgatag cctttttcaa caaacaatat tcgtgctgag
     1921 ttaaccagtg attgataggt actcttaaaa ttttcttgtt gatgattttt attttccatg
     1981 atagatttaa aataacatac cgtcagtatg tttatggtat catgatgatg tggtcgtgac
     2041 aatcttaaga acatttaggt tattttatgt atattgaaca gcattctcgc tatcaaaata
     2101 aagctaataa catccaatta agatatgatg ataagcagtt tcatacaacg gttatcaaag
     2161 atgttctatt atggattgaa cataatttag atcagtcttt actgcttgat gatgtggcga
     2221 ataaagcggg ttataccaag tggtattttc agcggctgtt caaaaaagta acaggggtca
     2281 cactggctag ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag ttgaggttga
     2341 cgaaaaaaac tatccttgag atcgcattaa aatatcaatt tgattcccaa caatctttta
     2401 cacgtcgatt taagtacatt tttaaggtta caccaagtta ttatcggcgt aataaattat
     2461 gggaattgga ggcaatgcac tgagagatcc cctcataatt tccccaaagc gtaaccatgt
     2521 gtgaataaat tttgagctag tagggttgca gccacgagta agtcttccct tgttattgtg
     2581 tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac gtttcgattt
     2641 ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg aacgcccgca
     2701 agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa acgctctgag
     2761 ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa gtctcggaag
     2821 gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg tgttcgaatt
     2881 tcaacaggta agttagttgc tagaacccat ggctcctttg ccgacgctga gtagatttta
     2941 ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc tttagagcga
     3001 gatttataca atagaatttg gcatgagatt ggattgcttt tagtcagcct cttatagcct
     3061 aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt ccagttttcc
     3121 gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta ccaacccagc
     3181 ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat gagcggtgtg
     3241 gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc tttctttgaa
     3301 cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc gtgtagtgcg
     3361 actgaagctc gcaataccat aagtcgtttt tgctcacgaa tatcagacca gtcaacaagt
     3421 acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta tacagcgagt
     3481 cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat gttatgtttt
     3541 gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt tttacagtca
     3601 agtaatgcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa ttcggggtaa
     3661 tgactccaac ttactgatag tgttttatgt tc
//
LOCUS       DAJVRP010000338         3206 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.guided.212, whole genome shotgun sequence.
ACCESSION   DAJVRP010000338 DAJVRP010000000
VERSION     DAJVRP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.guided.212"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..222)
                     /locus_tag="OQN12_004293"
     CDS             complement(<1..222)
                     /locus_tag="OQN12_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1656394.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLG"
     gene            complement(337..873)
                     /locus_tag="OQN12_004294"
     CDS             complement(337..873)
                     /locus_tag="OQN12_004294"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCS1656395.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            1205..1678
                     /locus_tag="OQN12_004295"
     CDS             1205..1678
                     /locus_tag="OQN12_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1656396.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1773..2297
                     /gene="sat2"
                     /locus_tag="OQN12_004296"
     CDS             1773..2297
                     /gene="sat2"
                     /locus_tag="OQN12_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1656397.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2355..3146
                     /gene="aadA1"
                     /locus_tag="OQN12_004297"
     CDS             2355..3146
                     /gene="aadA1"
                     /locus_tag="OQN12_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1656398.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          887 a          714 c          776 g          829 t
ORIGIN      
        1 ccctaagagt tcttgcacag tgcgaatatc acgccccgct tgtaatagat gcgtagcaaa
       61 cgagtgacga aatgtatgac aagtgacacg cttgctaacg atgcctgctt tttgtacggc
      121 tgccttcaat gcctttcgcg caacggagtc atgcagatga tggcggcata atttgccgtt
      181 atacgggtgg ttgcagagcg tgctggaggg aaagacaaac atccacgccg cttgtcgata
      241 agcagaaggg tatttgtgat ctaaagcaaa aggcagcgat ggccctacgc cttgtaagtt
      301 gtcgtcttgc tgaataagcc gcgcttgctc aatgagttat tttattgctg ggattaggcg
      361 cgtgggcagt aggctgtttc tgcttttccc acccttaccg tcatgcacag tgatgcagcc
      421 attatcaaaa tcaaaatctt taacccgcaa acgcaagcat tcattaatgc gcaaacctgc
      481 accatacagc agcgtaaaaa taacttggtt gcgagtatcc ataacctgca aaatgcgttg
      541 cacttcattt gcagagataa cagagggtag ccgtctaggc ttgcttgcag ggatataatc
      601 aatatcgccc aacggctgtt gtaaaaacct gttgtacaaa aaagctaggg catttaaagc
      661 gattttctgc gtgtttatgg ctacatgtct gctgtttgct aagctggata aaaacagcct
      721 gacctcttca ctgcccatgg tctgaggatg acgttttttg tgaaacagaa taaaacgctt
      781 aatccagtgc aggtaagttt tttcagtttt cagcgcataa cctttttgtc gcatatccgt
      841 gcgtatagaa tttaaaaatg gactgttaga cataaaacgc tccttgtctt gcaactgtct
      901 gcctatacag cctattctag ctgggattta aaaaagtgcc tgttttttac gcctagagat
      961 gcttgtttac cggtagagtt ttaatttaat gctaaataaa ttaaaatgtt atgagttctt
     1021 tgggtgagat aatgtgcatc gtgcaagcag gatagacggc atgcacgatt tgtaataaca
     1081 gagtgtcttg tatttttaaa gaaagtctat ttaatacaag tgattatatt aattaacggt
     1141 aagcatcagc gggtgacaaa acgagcatgc ttactaataa aatgttaacc tctgaggaag
     1201 aattgtgaaa ctatcactaa tggtagctat atcgaagaat ggagttatcg ggaatggccc
     1261 tgatattcca tggagtgcca aaggtgaaca gctcctgttt aaagctatta cctataacca
     1321 atggctgttg gttggacgca agacttttga atcaatggga gcattaccca accgaaagta
     1381 tgcggtcgta acacgttcaa gttttacatc tgacaatgag aacgtattga tctttccatc
     1441 aattaaagat gctttaacca acctaaagaa aataacggat catgtcattg tttcaggtgg
     1501 tggggagata tacaaaagcc tgatcgatca agtagataca ctacatatat ctacaataga
     1561 catcgagccg gaaggtgatg tttactttcc tgaaatcccc agcaatttta ggccagtttt
     1621 tacccaagac ttcgcctcta acataaatta tagttaccaa atctggcaaa agggttaaca
     1681 agtggcagca acggattcgc aaacctgtca cgccttttgt accaaaagcc gcgccaggtt
     1741 tgcgatccgc tgtgccaggc gttaggcgtc atatgaagat ttcggtgatc cctgagcagg
     1801 tggcggaaac attggatgct gagaaccatt tcattgttcg tgaagtgttc gatgtgcacc
     1861 tatccgacca aggctttgaa ctatctacca gaagtgtgag cccctaccgg aaggattaca
     1921 tctcggatga tgactctgat gaagactctg cttgctatgg cgcattcatc gaccaagagc
     1981 ttgtcgggaa gattgaactc aactcaacat ggaacgatct agcctctatc gaacacattg
     2041 ttgtgtcgca cacgcaccga ggcaaaggag tcgcgcacag tctcatcgaa tttgcgaaaa
     2101 agtgggcact aagcagacag ctccttggca tacgattaga gacacaaacg aacaatgtac
     2161 ctgcctgcaa tttgtacgca aaatgtggct ttactctcgg cggcattgac ctgttcacgt
     2221 ataaaactag acctcaagtc tcgaacgaaa cagcgatgta ctggtactgg ttctcgggag
     2281 cacaggatga cgcctaacaa ttcattcaag ccgacaccgc ttcgcggcgc ggcttaattc
     2341 aggagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2401 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2461 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2521 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2581 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2641 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2701 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2761 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2821 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2881 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2941 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3001 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3061 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac gtgaaaggcg
     3121 agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg
     3181 cggcgcggct taactcaagc gttaga
//
LOCUS       DAJVRP010000339         3203 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.guided.213, whole genome shotgun sequence.
ACCESSION   DAJVRP010000339 DAJVRP010000000
VERSION     DAJVRP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.guided.213"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..222)
                     /locus_tag="OQN12_004298"
     CDS             complement(<1..222)
                     /locus_tag="OQN12_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1656399.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLG"
     gene            complement(337..873)
                     /locus_tag="OQN12_004299"
     CDS             complement(337..873)
                     /locus_tag="OQN12_004299"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCS1656400.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            1205..1678
                     /locus_tag="OQN12_004300"
     CDS             1205..1678
                     /locus_tag="OQN12_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1656401.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1773..2297
                     /gene="sat2"
                     /locus_tag="OQN12_004301"
     CDS             1773..2297
                     /gene="sat2"
                     /locus_tag="OQN12_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCS1656402.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2355..3143
                     /gene="aadA1"
                     /locus_tag="OQN12_004302"
     CDS             2355..3143
                     /gene="aadA1"
                     /locus_tag="OQN12_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1656403.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          884 a          714 c          776 g          829 t
ORIGIN      
        1 ccctaagagt tcttgcacag tgcgaatatc acgccccgct tgtaatagat gcgtagcaaa
       61 cgagtgacga aatgtatgac aagtgacacg cttgctaacg atgcctgctt tttgtacggc
      121 tgccttcaat gcctttcgcg caacggagtc atgcagatga tggcggcata atttgccgtt
      181 atacgggtgg ttgcagagcg tgctggaggg aaagacaaac atccacgccg cttgtcgata
      241 agcagaaggg tatttgtgat ctaaagcaaa aggcagcgat ggccctacgc cttgtaagtt
      301 gtcgtcttgc tgaataagcc gcgcttgctc aatgagttat tttattgctg ggattaggcg
      361 cgtgggcagt aggctgtttc tgcttttccc acccttaccg tcatgcacag tgatgcagcc
      421 attatcaaaa tcaaaatctt taacccgcaa acgcaagcat tcattaatgc gcaaacctgc
      481 accatacagc agcgtaaaaa taacttggtt gcgagtatcc ataacctgca aaatgcgttg
      541 cacttcattt gcagagataa cagagggtag ccgtctaggc ttgcttgcag ggatataatc
      601 aatatcgccc aacggctgtt gtaaaaacct gttgtacaaa aaagctaggg catttaaagc
      661 gattttctgc gtgtttatgg ctacatgtct gctgtttgct aagctggata aaaacagcct
      721 gacctcttca ctgcccatgg tctgaggatg acgttttttg tgaaacagaa taaaacgctt
      781 aatccagtgc aggtaagttt tttcagtttt cagcgcataa cctttttgtc gcatatccgt
      841 gcgtatagaa tttaaaaatg gactgttaga cataaaacgc tccttgtctt gcaactgtct
      901 gcctatacag cctattctag ctgggattta aaaaagtgcc tgttttttac gcctagagat
      961 gcttgtttac cggtagagtt ttaatttaat gctaaataaa ttaaaatgtt atgagttctt
     1021 tgggtgagat aatgtgcatc gtgcaagcag gatagacggc atgcacgatt tgtaataaca
     1081 gagtgtcttg tatttttaaa gaaagtctat ttaatacaag tgattatatt aattaacggt
     1141 aagcatcagc gggtgacaaa acgagcatgc ttactaataa aatgttaacc tctgaggaag
     1201 aattgtgaaa ctatcactaa tggtagctat atcgaagaat ggagttatcg ggaatggccc
     1261 tgatattcca tggagtgcca aaggtgaaca gctcctgttt aaagctatta cctataacca
     1321 atggctgttg gttggacgca agacttttga atcaatggga gcattaccca accgaaagta
     1381 tgcggtcgta acacgttcaa gttttacatc tgacaatgag aacgtattga tctttccatc
     1441 aattaaagat gctttaacca acctaaagaa aataacggat catgtcattg tttcaggtgg
     1501 tggggagata tacaaaagcc tgatcgatca agtagataca ctacatatat ctacaataga
     1561 catcgagccg gaaggtgatg tttactttcc tgaaatcccc agcaatttta ggccagtttt
     1621 tacccaagac ttcgcctcta acataaatta tagttaccaa atctggcaaa agggttaaca
     1681 agtggcagca acggattcgc aaacctgtca cgccttttgt accaaaagcc gcgccaggtt
     1741 tgcgatccgc tgtgccaggc gttaggcgtc atatgaagat ttcggtgatc cctgagcagg
     1801 tggcggaaac attggatgct gagaaccatt tcattgttcg tgaagtgttc gatgtgcacc
     1861 tatccgacca aggctttgaa ctatctacca gaagtgtgag cccctaccgg aaggattaca
     1921 tctcggatga tgactctgat gaagactctg cttgctatgg cgcattcatc gaccaagagc
     1981 ttgtcgggaa gattgaactc aactcaacat ggaacgatct agcctctatc gaacacattg
     2041 ttgtgtcgca cacgcaccga ggcaaaggag tcgcgcacag tctcatcgaa tttgcgaaaa
     2101 agtgggcact aagcagacag ctccttggca tacgattaga gacacaaacg aacaatgtac
     2161 ctgcctgcaa tttgtacgca aaatgtggct ttactctcgg cggcattgac ctgttcacgt
     2221 ataaaactag acctcaagtc tcgaacgaaa cagcgatgta ctggtactgg ttctcgggag
     2281 cacaggatga cgcctaacaa ttcattcaag ccgacaccgc ttcgcggcgc ggcttaattc
     2341 aggagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2401 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2461 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2521 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2581 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2641 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2701 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2761 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2821 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2881 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2941 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3001 tgccggccca gtatcagccc gtcatacttg aagctaggca ggcttatctt ggacaagaag
     3061 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg aaaggcgaga
     3121 tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg
     3181 cgcggcttaa ctcaagcgtt aga
//
LOCUS       DAJVRP010000340         1189 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.guided.258, whole genome shotgun sequence.
ACCESSION   DAJVRP010000340 DAJVRP010000000
VERSION     DAJVRP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.guided.258"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            complement(<1..226)
                     /locus_tag="OQN12_004303"
     CDS             complement(<1..226)
                     /locus_tag="OQN12_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1656404.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQA"
     gene            488..946
                     /gene="gpt"
                     /locus_tag="OQN12_004304"
     CDS             488..946
                     /gene="gpt"
                     /locus_tag="OQN12_004304"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1656405.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1038..>1189
                     /locus_tag="OQN12_004305"
     CDS             1038..>1189
                     /locus_tag="OQN12_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1656406.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRM"
BASE COUNT          302 a          294 c          291 g          302 t
ORIGIN      
        1 gggcctgtaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt ttacgaatca
       61 ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa
      121 tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa
      181 aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg
      241 tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca
      301 agacaggctt gcgagatatg tattgagaat accactttat cccgcgtcag ggagaggcag
      361 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
      421 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
      481 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
      541 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
      601 cggtctggta ccgggggcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
      661 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
      721 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgctgt
      781 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
      841 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga tcgaacagcc
      901 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
      961 ggcattgccg ggcgttgttc tttttaactt caggcaggtt acaatagttt ccagtaagta
     1021 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
     1081 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
     1141 tgcagtcggc tcttgatggt aaaaccatcc ctcactggta tcgcatgat
//
LOCUS       DAJVRP010000341         1171 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 702033
            SAMEA8900929-rid15857173.guided.259, whole genome shotgun sequence.
ACCESSION   DAJVRP010000341 DAJVRP010000000
VERSION     DAJVRP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900929
            Sequence Read Archive: ERR6004908
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477822.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477822.1
            Genome Coverage       :: 38x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 02:53:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 149 of 321
            Pseudo Genes (incomplete)         :: 62 of 321
            Pseudo Genes (internal stop)      :: 138 of 321
            Pseudo Genes (multiple problems)  :: 27 of 321
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900929-rid15857173.guided.259"
                     /serovar="S. flexneri 1b"
                     /isolate="702033"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Pakistan"
                     /collection_date="2009-02-14"
     gene            264..923
                     /locus_tag="OQN12_004306"
     CDS             264..923
                     /locus_tag="OQN12_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCS1656407.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          315 a          255 c          276 g          325 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat a
//
